LOCUS       DATROD010000001        83736 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.001, whole genome shotgun sequence.
ACCESSION   DATROD010000001 DATROD010000000
VERSION     DATROD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 83736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83736
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.001"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(440..2050)
                     /gene="ftsY"
                     /locus_tag="VBC74_000001"
     CDS             complement(440..2050)
                     /gene="ftsY"
                     /locus_tag="VBC74_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEO6940990.1"
                     /translation="MGLFDRLFGHKKKDKELEIEASESVVLENEDSVIDKEESSNFSK
                     ESTLNRTSEVPVAEDDSFLELERDTALSESHQPVTSEIHPLESEDTSEIPVKEDDSFL
                     ELEDRAKTKVADTSEVGNVVPDSTTLSDNVSAKSEASFSDEEQSSDSQASEQFSETPL
                     QEEMSSGKTEVQTESEDTSAADAFLADYYAKRKAIEKEISSNSLSTDESELSEAQEVL
                     SQSQADTIKAESQEEKYNRSLKKTRTGFSARLNAFLSNFRRVDEEFFEELEEMLILSD
                     VGVNVATQLTEDLRYEAKLENAKKSEDLKRVIVEKLVEIYEKDGIYNEAINFQEGLTV
                     MLFVGVNGVGKTTSIGKLAHQYKSQGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVVT
                     GEKKADPASVVFDGMEKAVAQGVDVLLIDTAGRLQNKENLMAELEKIGRIIKRVVPDA
                     PHETLLALDASTGQNALSQAKEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVK
                     FIGFGEKIDDIGEFNSEDFMRGLLEGIL"
     gene            complement(2050..2874)
                     /locus_tag="VBC74_000002"
     CDS             complement(2050..2874)
                     /locus_tag="VBC74_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6940991.1"
                     /translation="MLDIKILALDLDGTLFTTDKKVSEENKVALKAAREKGIKVVITT
                     GRPLKAIGNLLEDLELVSDEDYSITFNGGLVQQNTGKILAKTAMTRQEVEDIHEELYQ
                     VGLPTDILSEGTVYSIANKGHHSQYHLANPLLEFIEVDDLEQVPKDVVYNKIVSVIDA
                     TYLDQQIAKLPDRLKVDYEMFKSRDIILELMPKGVHKAVGLELLTKHLGLDSSQVMAM
                     GDEANDLSMLEWAGLGVAMANGIPEAKATAKATTICNNDESGVAEAVGKYILSEEN"
     gene            complement(2874..3671)
                     /locus_tag="VBC74_000003"
     CDS             complement(2874..3671)
                     /locus_tag="VBC74_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6940992.1"
                     /translation="MIKLVATDMDGTFLDENGTYDKKRLANVLKKFKEQGIVFTAASG
                     RSLLSLEQLFADFRDQMAFIAENGSAAVLFNRLAYEQHLSREQYLDIIDHLSKSPYME
                     NNEYVLSGKDGAYILSDANPDYIEFITHYYDNLQKVSHFEDVDDIVFKVTANFTEETV
                     RQAEEWVNQAIPYATAVTTGFKSIDIILSSVNKRNGLEHLCEQYGIRAEEVLSFGDNI
                     NDLEMLEWSGKAIATENARPEVKEIADCIIGHHNDQAVMAYLESMVD"
     gene            complement(3762..7301)
                     /gene="smc"
                     /locus_tag="VBC74_000004"
     CDS             complement(3762..7301)
                     /gene="smc"
                     /locus_tag="VBC74_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEO6940993.1"
                     /translation="MFLKEIEMQGFKSFADKTKVEFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSAKSLRGGKMPDVIFAGTENRKPLNYAQVSVTLDNSDHFIENIADEVRVER
                     RIFRNGDSEYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEAIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQSKLEQTQGNLDRLEDIIYELDMQVQPLEKQASIAKRFLVL
                     DEERQGLHLSILIEDILQHQSDLTTVEEKLLTVREELATYYQQRQSLEDENQSLKQKR
                     HHLSEEIEVKQLALLDVTKLKSDLERQIDLIRLESSQKAEKKEEAGQRLAELEVKAKD
                     CNDQITQKNIELTTLSEKIAQIRSEIVSIESSLERFSTNPDQVIEKLREDFVTLMQEE
                     ADTSNALTALLADIENQKQASQAKSQEIQEVSKNLEVLKSNAKVALERFEAAKKNVRQ
                     LLSHYQDLGQTLQNLEGEYKNQQSILFDHLDEIKSKQARISSLESILKNHSNFYAGVK
                     SVLQAKDQLGGIIGAVSEHLSFDKHYQTALEIALGGSSQHIIVEDESAAKRSIAFLKK
                     NRQGRATFLPLTTIKPRELAQHYLSKLQSSQGFLGIASELVTYDQRLSNIFKNNLGLT
                     AIFDTVDNANVAARQLNYQVRLVTLDGTELRPGGSYSGGANRQNNTVFIKPELDNLKK
                     ELKQAQSKQLIQEKEVAALLEQLKEKQETLAQLKNDGEQARLEEQRADIEYQQLSEKL
                     ADLNKLYNGLQLSSGALEQTTSENEKNRLEKELEQFAIKKEELTTSIAQIKEDKDSIQ
                     EKVNNLTTLLSEAQLEERDLLNEQKFERANCTRLEITLSEIKRDISNLQTLLSHQDSQ
                     LDKEELPRIEKQLLQANKRRENDEEKLVSLRFELEDCEAALDDLAASLAKEGQKNESL
                     IRQQAQLESQCEQLSQQLMIFSRQLSEDYQMTLDEAKVKANVLEDILMAREQLKSLQA
                     KIKALGPVNIDAIAQFEEVHERLTFLNTQRDDLVHAKNLLLETITDMDDEVKTRFKST
                     FEAIRHSFKETFVQMFGGGSANLILTEGDLLSAGVDISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKSSQFIVVTHRKGT
                     MSAADSIYGVTMQESGVSKIVSVKLKEAQEMTN"
     gene            complement(7309..7995)
                     /locus_tag="VBC74_000005"
     CDS             complement(7309..7995)
                     /locus_tag="VBC74_000005"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEO6940994.1"
                     /translation="MKELRSKLEKDYGIVFANQELLDTAFTHTSYANEHRLLNISHNE
                     RLEFLGDAVLQLLISQYLFTKYPQKAEGDLSKLRSMIVREESLAGFSRLCGFDHYIKL
                     GKGEEKSGGRNRDTILGDLFEAFLGALLLDKGVEVVHAFVNKVMIPHVEKGTYERVKD
                     YKTSLQELLQSHGDVKIDYQVTNESGPAHAKEFEVTVSVNQENLSQGIGRSKKAAEQD
                     AAKNALATLQ"
     gene            complement(8170..8538)
                     /locus_tag="VBC74_000006"
     CDS             complement(8170..8538)
                     /locus_tag="VBC74_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEO6940995.1"
                     /translation="MKKIFYISLGFISLGIGIAGIVLPVVPTTPLVLLSAFCFSRSSE
                     KFDIWLRQTKVYKYYAADFVESRSIAPARKKSMIWQIYILMGISIYFAPLMWLKLGLL
                     IGTIVGTYVLFYVVPDKIDE"
     gene            complement(8541..9350)
                     /locus_tag="VBC74_000007"
     CDS             complement(8541..9350)
                     /locus_tag="VBC74_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO6940996.1"
                     /translation="MSEIGFKYSILASGSTGNCFYIETPQKRLLIDAGLTGKKVTSLL
                     AEINRKPEDLDAILVTHEHSDHIKGVGVLARKYHLDIYANEQTWKVMDERNMLGKVDV
                     SQKHVFGRGKTLTFGDLDIESFGVSHDAVDPQFYRMMKDDKSFVMLTDTGYVSDRMAS
                     LIENADGYLIESNHDIEILRSGSYPWTLKQRILSDKGHLSNEDGSETMIRTIGNRTKH
                     IYLGHLSKENNIKELAHMTMENNLMRADFGVGTDFSVHDTSPDSATPLTRI"
     gene            complement(9354..10703)
                     /gene="vicK"
                     /locus_tag="VBC74_000008"
     CDS             complement(9354..10703)
                     /gene="vicK"
                     /locus_tag="VBC74_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEO6940997.1"
                     /translation="MNNSAANIRSFELALLFLLVFVAVYFVYLAVRDFKMSKDIRLLN
                     WKVRDLIAGNYSDSILIQGDADLVELGESLNDLSDVFRMAHDNLEQEKNRLASILTYM
                     TDGVLATDRSGKIVMINETAQQQFNLAYDEALSMNIVDMLGSGSPYSFQDLVSKTPEV
                     VLNRRDENGEFVTLRIRFALNRRESGFISGLVAVSHDATEQEKEERERRLFVSNVSHE
                     LRTPLTSVKSYLEALDEGALNEEVAPSFIKVSLDETNRMMRMISDLLSLSRIDNEVTH
                     LDVEMTNFTAFMTSILNRFDQIRNQKTVTGKVYEIVRDYPLKSIWVEIDTDKMTQVID
                     NILNNAVKYSPDGGKITVNLRTTKTQMILSISDQGLGIPKKDLPLIFDRFYRVDKARS
                     RQQGGTGLGLSIAKEIVKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVTYEEWEDVED"
     gene            complement(10696..11406)
                     /locus_tag="VBC74_000009"
     CDS             complement(10696..11406)
                     /locus_tag="VBC74_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6940998.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYETATAFDGREALVQYAEF
                     QPDLIILDLMLPELDGLEVAKEVRKTSHIPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTENIETAVAEESAQNASSDITIGELQILPDAFIAKKRGEEIE
                     LTHREFELLHHLATHIGQVMTREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPGR
                     PEYILTRRGVGYYMKSYE"
     gene            11746..12501
                     /locus_tag="VBC74_000010"
     CDS             11746..12501
                     /locus_tag="VBC74_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6940999.1"
                     /translation="MSLISYKNVNKYYGDYHALRQINLEIEPGQVVVLLGPSGSGKST
                     LIRTMNALESIDDGSLVVNGHELANISSKELVNLRKEVGMVFQHFNLYPHKTVLENIT
                     LAPIKVLKQSKKEAMEIAEKYLKFVNMWERKDSYPSMLSGGQKQRIAIARGLAMHPKL
                     LLFDEPTSALDPETIGDVLSVMQKLANDGMNMVVVTHEMGFAREVADRIIFMADGEIL
                     VDTTDVQDFFDNPTEPRAKQFLRNIINHTSTDL"
     gene            12513..13313
                     /locus_tag="VBC74_000011"
     CDS             12513..13313
                     /locus_tag="VBC74_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6941000.1"
                     /translation="MLRRKRLTLYLLSCIFIFLLFYPNSTSANQLSEIKKSGVLKVGV
                     KQDVPNFGYYNAETNQYEGMEIDIAKKIAKSLGVKPVFVPTTAQTREPLMDNGQIDIL
                     IATYTITPERKANYNISKAYYHDEIGFLVRKNSHIKTIKELDGKHIGVAQGATTKVNL
                     EKYAKEHKLKFSYAQLGSFPELAISLYANRIDAFSVDKSILSGYLSPHTTILKEGFNT
                     QEYGIATSKQDKVLIPYVNKLLVSWEKDGSLKHIYQKFKLKPAKVKKE"
     gene            13326..14021
                     /locus_tag="VBC74_000012"
     CDS             13326..14021
                     /locus_tag="VBC74_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6941001.1"
                     /translation="MTLLNISPFAISRWGAFFNHFDLFFKGFLYTLGISFGALLLALI
                     LGILSGGLSTSKSKVGKLISRIYVEVFQNTPLLVQMVFVYYGLAIISNGHIMISAFFT
                     AVLCVGLYHGAYISEVIRSGIEAVPKGQTEAALAQGFTANQTMQLIILPQAIRTILPP
                     MTNQVVNLIKNTSTVAIISGADIMFLAKAWAYDTTNYIPAFAGAAIFYFVICFPLASW
                     ARKQEELNKKTYS"
     gene            14033..14683
                     /locus_tag="VBC74_000013"
     CDS             14033..14683
                     /locus_tag="VBC74_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6941002.1"
                     /translation="MSSVLTLTNILFLLQGFGLTLYISFISILLSMFFGTLLAIMRNS
                     KNPIWKLIASIYIEFVRNVPNLLWIFIIFLVFQMKSVSAGITSFTIFTSAALAEIIRG
                     GLNGVDKGQTEAGLSQGFTYLQVFIIIIFPQAFRKMLPAIISQFVTVIKDTSLLYSVI
                     AIQEIFGKSQILMGRYFEAGQVFTLYAIITAVYFITNFIISSFSRKLSKRWEQATE"
     gene            complement(14901..16094)
                     /locus_tag="VBC74_000014"
     CDS             complement(14901..16094)
                     /locus_tag="VBC74_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6941003.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKTSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNEQRANVKKST
                     MKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKEKGFSYNTINNHKRSLKASFY
                     IAIQDDCVRKNPFDFKLSEVLENDTKEKVALTEEQEQALLSFIKTDNVYHKYYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKNGTRQVPLSR
                     ETIQAFQRVMKKRPKAEPFVIDGRSNFLFVNHKGKPKVAIDYNALFVRMVKKYNKQHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(16173..16379)
                     /locus_tag="VBC74_000015"
     CDS             complement(16173..16379)
                     /locus_tag="VBC74_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO6941004.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16372..17514)
                     /locus_tag="VBC74_000016"
     CDS             complement(16372..17514)
                     /locus_tag="VBC74_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO6941005.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYKNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            18026..18661
                     /locus_tag="VBC74_000017"
     CDS             18026..18661
                     /locus_tag="VBC74_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6941006.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18815..18892
                     /locus_tag="VBC74_000018"
                     /pseudo
     CDS             18815..18892
                     /locus_tag="VBC74_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            19137..19253
                     /locus_tag="VBC74_000019"
     CDS             19137..19253
                     /locus_tag="VBC74_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEO6941007.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            19269..21188
                     /gene="tet(M)"
                     /locus_tag="VBC74_000020"
     CDS             19269..21188
                     /gene="tet(M)"
                     /locus_tag="VBC74_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO6941008.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            21307..21474
                     /locus_tag="VBC74_000021"
     CDS             21307..21474
                     /locus_tag="VBC74_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO6941009.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(21534..21887)
                     /locus_tag="VBC74_000022"
     CDS             complement(21534..21887)
                     /locus_tag="VBC74_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6941010.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            22392..22715
                     /locus_tag="VBC74_000023"
     CDS             22392..22715
                     /locus_tag="VBC74_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO6941011.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYLW
                     EFRYF"
     gene            22694..23023
                     /locus_tag="VBC74_000024"
     CDS             22694..23023
                     /locus_tag="VBC74_000024"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941012.1"
                     /translation="MGISLFLELPAIVSYWVVTVMCFVAMGVSTMFVVQIYTLVQTQT
                     PPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVASIASIMISIYSKR
                     IFQKLEI"
     gene            23250..23669
                     /locus_tag="VBC74_000025"
     CDS             23250..23669
                     /locus_tag="VBC74_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6941013.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDRKISELYHVSRSAICSRRTRGLRKLKMLLSEGK"
     gene            23674..23913
                     /locus_tag="VBC74_000026"
     CDS             23674..23913
                     /locus_tag="VBC74_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6941014.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(24098..24664)
                     /locus_tag="VBC74_000027"
     CDS             complement(24098..24664)
                     /locus_tag="VBC74_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO6941015.1"
                     /translation="MIKKNKVFLGVLLVLVVILGGGVLFYQSQFQKTTNQALAIAYKD
                     AKVAKKDVIHQKIDKEFENFRGSYEIEFNTKSAEYSYHVDAKTGQILERDMDNNGFSK
                     STSQSSSSSSQKSHKISQEEAKKIAFKDANIEESEVSNLKIKEEIENGKSVYDIDFVD
                     LKNKNEVDYQIDAETGKIIERSRDHMND"
     gene            complement(24831..25850)
                     /locus_tag="VBC74_000028"
     CDS             complement(24831..25850)
                     /locus_tag="VBC74_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEO6941016.1"
                     /translation="MAKTALNIPFTVEGIENYYQNIKMILSLADKWSIPKDDLLLFLK
                     GLKAIDDIKVGSPIFKYFWTSLSLQAPLKALEFVLEQAKMPTELSGELSETQYLVAQF
                     SDELAPHDDFWIALSQVIYDSFPGNSLAEDTVLNRKLHQFRYLISSQQAQYVRRYFKD
                     VGMTDRDALVNYLSCLREPDSIAYYESARLHNKRRRNGEIFGFPDDEPVINSKLLISF
                     HTEFIIDDKGNFLNEIDAEVITRNGIINGASFNYAFKNNTRHKELDVDPVKLDPKFRN
                     DMTRGYRSPNLSRRKWFFFKEEDYDCSYFNKKGYYAFGRRSAKQSVDKQVKYLKKAVQ
                     KMRIN"
     gene            complement(25948..26652)
                     /locus_tag="VBC74_000029"
     CDS             complement(25948..26652)
                     /locus_tag="VBC74_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6941017.1"
                     /translation="MRTVLVVQGDDETIELLRSYLEGALYKVVMASDGEEAFSLFQQH
                     QIDLAIIDITLPKIDGYELTRLIRQDSQIPIIMLAAKTTDMDRILGLNIGADDFITKP
                     FNSLEVLARINSQLRRYYEFNSLAKPKNQFIKIGELELDEEHVELTKNGKHIKLTATE
                     FKILHILMSSPGKIYTKTQLYEKINGRYLEGDETTIMVHISNIRDKIEDDSKYPKYIK
                     TLRGVGYKIEKPQYLV"
     gene            complement(26873..28816)
                     /gene="thrS"
                     /locus_tag="VBC74_000030"
     CDS             complement(26873..28816)
                     /gene="thrS"
                     /locus_tag="VBC74_000030"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEO6941018.1"
                     /translation="MIKITFPDGAIREFESGITTFEIAQSISNSLAKKALAGKFNGQL
                     IDTTRAIEEDGSIEIVTPDHEDALGVLRHSAAHLFAQAAKRLFPDLCLGVGPAIHDGF
                     YYDTDNKSGQISNDDLPRIEEEMKKIVKENHPCIREEISKEEALELFKDDPYKVELIS
                     EHAEDGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNAMMQRVYGT
                     AWFDKKDLKAYLKRREEAKERDHRKLGKELDLFMVNPEVGQGLPFWLPNGATIRRELE
                     RYIVDKEIASGYQHVYTPPMASVEFYKTSGHWDHYREDMFPTMDMGDGEEFVLRPMNC
                     PHHIEVYKHHVHSYRELPIRIAELGMMHRYEKSGALTGLQRVREMTLNDAHIFVTPEQ
                     IKDEFLKALNLIAEIYEDFNLTDYRFRLSYRDPEDKHKYYDNDEMWENAQAMLKEAMD
                     DFGLDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPIMIHRGGISTMERFTAILIETYKGAFPTWLAPQQVSVIPISNEAHIDYAWEVARA
                     LKDRGIRAEVDDRNEKMQYKIRAAQTQKIPYQLIVGDKEMEEKAVNVRRYGSKATETK
                     SIEEFVESILADIARKSRPDEVK"
     gene            complement(29273..30607)
                     /locus_tag="VBC74_000031"
     CDS             complement(29273..30607)
                     /locus_tag="VBC74_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO6941019.1"
                     /translation="MRIGLFTDTYFPQVSGVSTSIRTLKEGLEKEGHEVYIFTTTDRN
                     VKRFEDPTIIRLPSVPFISFTDRRVVYRGLISAYRIAKDYELDIIHTQTEFSLGLLGK
                     LVAKALRIPVVHTYHTQYEDYVGYIAKGKLIKPSMVKYIMRTYLSDLDGVICPSRIVL
                     NLLDGYGVKIPKQVIPTGIPVENYRREDISEETIKNLRTELGLADNDTMLLSLSRVSF
                     EKNIQAILMHLSAVVDENPHVKLVIVGDGPYLSDLKELVHSLELENSVIFTGMVEHSQ
                     VAIYYKACDFFISASTSETQGLTYIESLASGRPIIAQSNPYLDDVISDKMFGTLYKKE
                     SDLADAILDAIAETPKMTQEAYEQKLYEISAENFSKSVYAFYLDFLISQKASVKEKVS
                     LTIGNKDSHSTLRFVRKAVYLPKKVFTFTGRASKKVVKAPKRRISSIRDFLD"
     gene            complement(30609..31607)
                     /locus_tag="VBC74_000032"
     CDS             complement(30609..31607)
                     /locus_tag="VBC74_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6941020.1"
                     /translation="MKVLLYLEAEEYLKKSGIGRAIKHQEKALQIAGIDYTTNPTDDF
                     DLVHMNTYGIRSWLLMSKAKKTGKKVIMHGHSTEEDFRNSFIGSNLVSPLFKWYLCRF
                     YQKADAIITPTDYSKQLIKAYGIKKPIFVLSNGIDLSRYQRSEKKEAAFRHYFHLSKD
                     DKVVMGAGLYFMRKGIDQFVEVAAKMPDIRFIWFGETNKWVIPRKVRQIVTKQHPSNV
                     TFAGYIKGDVYEGAMSASDAFFFPSREETEGIVVLEALASHQHVVLRDIPVYHGWVTE
                     DSVELATDVDGFVEKLDKVLSGKSDKIKEGYRVAESRSIERIAHELASVYQKVMEL"
     gene            complement(31652..33118)
                     /locus_tag="VBC74_000033"
     CDS             complement(31652..33118)
                     /locus_tag="VBC74_000033"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEO6941021.1"
                     /translation="MTNELIMQAFEWYLPSDGNHWKKLEESISDLKKLGISKIWLPPA
                     FKGTSSDDVGYGVYDLFDLGEFDQNGTIRTKYGRKEEYLKLIKSLKANGIKPFADIVL
                     NHKANGDHKEKFQVIKVNPENRQEALSEPYEIEGWTGFDFPGRQGEYNDFKWHWYHFT
                     GLDYDAKNNETDIFMIVGDNKGWADDDLIDDENGNFDYLMYNDIDFKHPEVIKNLQDW
                     AKWFIETTGIEGFRLDAVKHIDSYFIQTFINDIRTKIKPDLEVFGEYWKSDQTSMKDY
                     LEATQFQFSLVDVTLHMNFFDASHQNRDFDMRTIFDDSLVIDNPEYAVTFVENHDTQS
                     GQALESRVEDWFKPLAYGLILLRQQGTPCLFYGDYYGIQGEFGQPSFKEVIDKMAELR
                     QNYVFGEQVDYFTHSNCIGWTCLGDEEHNSCLAVVLTNGDQGWKHMEVGEIYAGKTFV
                     DYLGNCEQEVVIGDDGWGDFLVASASISAWVPKIEEAN"
     gene            complement(33250..34254)
                     /gene="ccpA"
                     /locus_tag="VBC74_000034"
     CDS             complement(33250..34254)
                     /gene="ccpA"
                     /locus_tag="VBC74_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEO6941022.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILARGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPIVLAGTVDLEHQLPS
                     VNIDYKAAAVDVIDILAGNHKDIAFVSGPLIDDINGKVRLAGYKEGLKKNGLNFKEGL
                     VFEANYRYAEGFALAQRVINAGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPIAQYTRPNLTSISQPVYDLGAVSMRMLTKIMHKEELEEKEIVLNHGIVKRGTTNN
                     "
     gene            34464..35549
                     /locus_tag="VBC74_000035"
     CDS             34464..35549
                     /locus_tag="VBC74_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO6941023.1"
                     /translation="MSKLNRIRHHLHSVQAELAVFSDPVTVNYLTGFFCDPHERQMFL
                     FVYEDRDPILFVPALEVSRAKQSVPFPVFGYIDSENPWQKIASNLPSFSVSKVLAEFD
                     NLNVTKFQGLQTVFDGHFENLTPYIQNMRLIKSRDEIEKMLVAGEFADKAVQVGFDNI
                     SLNNTETDIIAQIEFEMKKQGINKMSFDTMVLTGNNAANPHGIPGTNKIENNALLLFD
                     LGVETLGYTSDMTRTVAVGKPDQFKKDIYHLCLEAHQAAIDFIKPGVLASEVDAAARN
                     VIEKAGYGQYFNHRLGHGLGMDVHEFPSIMAGNDMEIQEGMCFSVEPGIYIPDKVGVR
                     IEDCGYVTKTGFEVFTKTPKELLYFEG"
     gene            complement(35607..37397)
                     /locus_tag="VBC74_000036"
     CDS             complement(35607..37397)
                     /locus_tag="VBC74_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEO6941024.1"
                     /translation="MTHLVDLTKKPFNLNQEAIEWIEKTINEMTLDEKIGQLFFNMGA
                     SRSEEYLTDVLDRYHIAAVRYNRGSSSEIYDQNLILQTKSKLPMLIAANTEAGGDGAV
                     TDGTKVGDEIKVAATNDPKYAYEMGRIAGMEASAVGCNASFSPIVDLTRNWRNPIIAS
                     RNWGANVDQIISLSKEYMKGIMQYNIVPFAKHFPGDGIDERDHHLSFASNPMSKEEWM
                     STFGRIYGELADAGLPGVMAGHIHLPNVEKEMHPERDLDDMLPASLNKTLLDELLRGE
                     LGYNGAIVTDASHMVGMTASMPRRDLLPTAIEAGCDLFLFFNDPDEDIQWMKEGYEKG
                     ILTEERLHDALRRTLGLKAKLGLHNYEGRRQELFMPKDKAMALINTLESQKIADEVAD
                     KAVTLVKDKQKDIFPVNPERYRHILLVNVEGYKGGFGAMIAGNKQRASDYMKELLEAR
                     GHEVTVWESTEERIMKLPQEERAAAIANVYAQKQPIANLTEHYDLIINLVDVNAGGTT
                     QRIIWPAAKGTPDQPFYVHEIPSIVISVQHAFALADMPQVGTYINAYDGLPSTISAVV
                     AKLAGESEFTGVSPVDAYCGLIDTHLWRQC"
     gene            complement(37413..38225)
                     /locus_tag="VBC74_000037"
     CDS             complement(37413..38225)
                     /locus_tag="VBC74_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6941025.1"
                     /translation="MVDTNHKIAFCIDSDGCAMDTMTYKHQLFFGPIAADIFGISEQE
                     RTSFLKEWDRVNLYSRTRGVNRFVGLVMGLEFAGVEHIDKLKEWVETTDSLSNNSLEL
                     ALANDFSRDLEKALEWSNEVNRQIKNYEGDSLAFSGVLESLERLSQFGNVYVVSSANK
                     EAVWQEWEEQGLLKYVDDIYCQDRGKKEDVIATLIASGYCRETILMVGDSLGDLAAAE
                     QNGIAFYPVLVGKEVKSWEILREDIGEAFAKGQFEQQRQKESINTFWANLDN"
     gene            complement(38378..39217)
                     /locus_tag="VBC74_000038"
     CDS             complement(38378..39217)
                     /locus_tag="VBC74_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO6941026.1"
                     /translation="MSKTITFTNKVVVITGAGGVLCGYMAKEFAKAGAKVALLDLNQE
                     AAQTFADEIVEEGGIAKAYKANVLSKENLEEVHQAVLEDLGPTDILVNGAGGNNPKAT
                     TDNEFHELDLPSETKTFFELDEAGISFVFNLNYLGTLLPTQVFAQDMVGREGANIINI
                     SSMNAFTPLTKIPAYSGAKAAISNFTQWLAVHFSKVGIRCNAIAPGFLVTNQNRSLLF
                     TEDGQPTARAEKILNNTPMGRFGEASELIGGLFFLADEKSSSFVNGVVLPIDGGFAAY
                     SGV"
     gene            complement(39341..40387)
                     /gene="uxuA"
                     /locus_tag="VBC74_000039"
     CDS             complement(39341..40387)
                     /gene="uxuA"
                     /locus_tag="VBC74_000039"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEO6941027.1"
                     /translation="MEMSFRWYGEDDPVTLENIGQIPTMKGIVTAIYDVPVGEVWSRE
                     RIQQLKEKVEAAGLKISVIESVPVHEDIKLGRPTRDLLIDNYIQTVKNLAAEGIDTIC
                     YNFMPVFDWTRTDLAYQYPDGSTALIFDETVSKKMDPVNGELSLPGWDASYSKEEMKA
                     IMDAYAEIDEEKLWENLTYFIKRIIPEAEAVGVKMAIHPDDPPYSIFGLPRIITGLEA
                     IERFVKLYDSKSNGITLCVGSYASDPQNDVLEISRRAFELDRVNFVHARNIKLGDGKS
                     FKESAHPSEYGSIDMYEVIKLCHEFGFEGAIRPDHGRMIWGETGRPGYGLYDRALGAT
                     YVSGLYEAVIKGSK"
     gene            complement(40405..41805)
                     /gene="uxaC"
                     /locus_tag="VBC74_000040"
     CDS             complement(40405..41805)
                     /gene="uxaC"
                     /locus_tag="VBC74_000040"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEO6941028.1"
                     /translation="MAFNTETFMLKNQAAIQLYEEVKRQPIFDYHCHLDPKDIFEDHI
                     FDNIVDLWLGGDHYKWRLMRANGISEAEITGPASNLEKFKAFARTLERAYGNPVYHWS
                     AMELKNIFGVNEILTESNAEEIYHRLNHFLKEHKISPRRLIADSKVMFIGTTDHPLDT
                     LEWHKKLAADESFKTVVAPTFRPDEAFIEHRHFVDFITKLGDITQKEITDFSTFIAAM
                     EERIAYFAQNGCRASDISFTEIVFEQTDELELNDLFNKVCEGYIPNQSEISKWQTAVF
                     MELCRLYKKYGFVTQVHFGALRNNHSTIFEKLGADVGVDSLGDQVALTVNMNRLLDSL
                     VKKDSLPKMIWYNLNPAYNIAVANTLANFQANELGVRSYLQFGAGWWFADTKLGMISQ
                     MNALAEQGMLANFIGMLTDSRSFLSYQRHDYFRRILCTYLGEWIEEGEVPEDYQALGS
                     MAKDIAYQNAVNYFKN"
     gene            complement(41822..42439)
                     /gene="eda"
                     /locus_tag="VBC74_000041"
     CDS             complement(41822..42439)
                     /gene="eda"
                     /locus_tag="VBC74_000041"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO6941029.1"
                     /translation="MLNQLKNNYFFAVIRGKSSEDALEIAKHAILGGIRNIEVTFSTP
                     EASKVIKQLSDDFKNNKEIIVGAGTVMTTELAKEAIDAGAKFLVSPHFDSDIANLANE
                     NKVYYFPGCATATEIVVARKYKCQIIKLFPGGVVGSGFIKDIHGPIPDVDLMPSGGVS
                     VSNVVEWRKAGAVAVGVGSALSSKVATEGYDSVTKIAKQFVSALD"
     gene            complement(42556..43227)
                     /locus_tag="VBC74_000042"
     CDS             complement(42556..43227)
                     /locus_tag="VBC74_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEO6941030.1"
                     /translation="MARPLVEQTADRLLHLILEREYPVGAKLPNEYELAEDLDVGRST
                     IREAVRSLATRNILEVRQGSGTYISSKKGVSEDPLGFSLIKDTDRLTSDLFELRLLLE
                     PRIAELVAYRITDDQLQLLEKLVGDIEDAVHAGDPKHLLLDVEFHSMLAKYSGNIAMD
                     SLLPVINQSIHLINANYTNRQMKSDSLEAHREIIKAIREKNPVAAHDAMLMHIMSVRR
                     SALKA"
     gene            complement(43256..45055)
                     /gene="uidA"
                     /locus_tag="VBC74_000043"
     CDS             complement(43256..45055)
                     /gene="uidA"
                     /locus_tag="VBC74_000043"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEO6941031.1"
                     /translation="MLYPLLTKTRNTYDLGGIWNFKLGEHNPNELLPSDEVMVIPTSF
                     NDLMVSKEKRDYIGDFWYEKVIEVPKVSEDEEMVLRFGSVTHQAKIYVDGVLVGEHKG
                     GFTPFEVLVPECKYNNEKIKVSICANNVLDYTTLPVGNYSEIIQEDGSIKKKVRENFD
                     FFNYAGVHRPLKLMIRPKNHIFDITITSRLSDDLQSADLHFLVETNQKVDEVRISVFD
                     EDNKLVGETKDSRLFLSDVHLWEVLNAYLYTARVEIFVDNQLQDVYEENFGLREIEVT
                     NGQFLLNRKPIYFKGFGKHEDTFINGRGLNEAANLMDLNLLKDMGANSFRTSHYPYSE
                     EMMRLADRMGVLVIDEVPAVGLFQNFNASLDLSPKDNGTWNLMQTKAAHEQAIQELVK
                     RDKNHPSVVMWVVANEPASHEAGAHDYFEPLVKLYKDLDPQKRPVTLVNILMATPDRD
                     QVMDLVDVVCLNRYYGWYVDHGDLTNAEVGIRKELLEWQDKFPDKPIIITEYGADTLP
                     GLHSTWNIPYTEEFQCDFYEMSHRVFDGIPNLVGEQVWNFADFETNLMILRVQGNHKG
                     LFSRNRQPKQVVKEFKKRWMTIPHYHNKKNSVK"
     gene            complement(45072..46097)
                     /locus_tag="VBC74_000044"
     CDS             complement(45072..46097)
                     /locus_tag="VBC74_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO6941032.1"
                     /translation="MAKIISLGEVLLRLSPPQYHTLMQANHLKCQFGGSELNVLASLA
                     QLGYHVGLVSALPDNDLGKMASQFILSQQISPAAIIKKEGRLGIYYYEQGFSVRTNKV
                     IYDRNYSSFWESTLSDYDFTSIFKGVDWFHVSGITPALTKDLYEVTRFLMTKAKEGGV
                     KVSIDLNFRESLWSSFQEAREQLSPLLGLLDVCFGLEPIYLAGESEDLKDELGLSRPY
                     LDIELLEKITQKIVQEYGLDYIAFTQREMEYTNQYMLKSYLYHNNMLYQTDKTGVEVL
                     DRVGTGDAFAAGLIHALLEKETPQRALEIAMATFKYKHTIQGDINIMTRDDIAYLIEK
                     ETNDIKR"
     gene            complement(46164..47714)
                     /locus_tag="VBC74_000045"
     CDS             complement(46164..47714)
                     /locus_tag="VBC74_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEO6941033.1"
                     /translation="MEKTMDMEQREEVVSYNRAKVWQLVMFAMNNASTNIYLFTFMFV
                     TYFSTGVLGSAAIFVSQIMGYIRIFDGFIDPAIGIMIDKTDTKFGKYRPILIIGNVIT
                     ALSLIFLLALRGVDENIRFPLFILVLIIHKIGYSMQQTITKAGQTALTNDPKQRPIFN
                     IVDAVMTTSLMTGGQFVVSVFLVPKFGNFTPQFFNVLIFGTILISAILAIVAIIGIWA
                     KDRKEFFGLGENTQKTALKDYWKVLKGNKPLQILSIAAALVKFAIQFFGDSVVMVLLF
                     GILFGNYALSGQFSLLFIVPGVIINILFSTIARKKGLRFSYVRAIQIGMIGLLAFGAV
                     LYVGKPGDLSLTSLNLYTILFIVTNIIARYASQAPASLVLTMGADISDYETSESGRYV
                     SGMIGTIFSLTDSIASSFAPMVVGFVLAGIGFSKSFPTIETPLPPDLKMAAISILVAI
                     PFIALSIALLLMKFYKLDKEEMVRIQEKIQVMKAATTKERAKDIARNVPLTDMDYVDV
                     TKYPIDKD"
     gene            complement(47921..48913)
                     /locus_tag="VBC74_000046"
     CDS             complement(47921..48913)
                     /locus_tag="VBC74_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEO6941034.1"
                     /translation="MKLKVFNVREEEATLAQDWANRNHVELSMSEGPLTLETVNEVEG
                     FDGIANAQIEPLDDAIYPLLKEMGIKQIAQRSAGVDMYNLELAKQHGIIISNVPSYSP
                     ESIAEFTVTIALNLIRKVELIRANVREQNFSWTLPIRGRVLGNMTVAIIGTGRIGLAT
                     AKIFKGFGCRVIGYDIYHNPMADGILEYVNSVEEAVEEADLVSLHMPPTAENTHLFNL
                     DMFKQFKKGAILMNMARGALVETKDLLEALDQGLLEGAGIDTYEFEGPYIPKNCQGQD
                     ISDKDFLRLINHPKVIYTPHAAYYTDEAVKNLVEGALNACVEVIETGTTTTKVN"
     gene            complement(48927..49985)
                     /locus_tag="VBC74_000047"
     CDS             complement(48927..49985)
                     /locus_tag="VBC74_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO6941035.1"
                     /translation="MSDVVEKQTTKSFIMNVLNGLALGTVIVLIPGAILGELMKALLP
                     MWSGFATLIAATAVATSMMGLVIGIMVGLNFKFNPIQSASLGLAVMFAGGAATFLKGA
                     IMLKGTGDIINMGITAALGVLLIQFLSDKTKSFTLIVIPTVTLLLVGGVGHVLLPYVK
                     MITTMIGQGIASLLGLQPVLMSILIAMIFCFLIVSPITTVGIALAISLSGIGSGAANL
                     GICAASFGLCMAGWSVNSKGTALAHVLGSPKISMANVLAKPKIMLPMISSAAILGILG
                     ALFNIQGTPASAGFGISGLIGPINALNLAKGGWSVMNMLLIIIIFVAAPIILNFIFNY
                     LFIKVLKIIDPMDYKLDI"
     gene            50178..51062
                     /locus_tag="VBC74_000048"
     CDS             50178..51062
                     /locus_tag="VBC74_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6941036.1"
                     /translation="MDIRQLTYFIAVAEAKNYSRAAKSLFVTQPTLSQSIKKLEAELN
                     TILFLQNGRQLALTEAGEILYEKGQLLMTNVNQMVTEIQQLNQEKKEGIRVGLTSLFA
                     IQFMKQISTFMATHSNVEVSLIQDGSRKLQELLAKGKIDIGLLSFPSTRNDITIEPLQ
                     TSTKGYKVSIVMPKSHPLATLPEIELNDLRDYKVASLNEHYMLGEMLPRKCRSLGFDP
                     HIVFKHNDWEVLIHSLQDLNAVTILPSEFESISQVQDLCWVPLKDKNNFYPIGIAYRN
                     DTSFSPMIEEFLSLLKTN"
     gene            complement(51099..51917)
                     /locus_tag="VBC74_000049"
     CDS             complement(51099..51917)
                     /locus_tag="VBC74_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATPase"
                     /protein_id="HEO6941037.1"
                     /translation="MILKICRAAYSLQWGGVYQLALLDYPRIKAFELERIGAFIAYEK
                     QYKRKTEIQCDDKHLLAKIVHFLKYNSFTFPYIPKYREAAATFNEDGISLTSDFLSHT
                     CTIETAKLIFKEGKILSAVKAFNKPAEVLVKDKRNAAGDPKDYFDYVMLNWSNTNSGY
                     RLVMERLLGKAPSEQELTVGFKPGVSFHFTYQDIINHPDSIFDGYHPAKIKNQLSLAE
                     HLVACVIPKHYQEDYQSLVPNDLKHRVYYLDYCNETLYEWNQKVYDFLCHLENK"
     gene            complement(52081..52854)
                     /gene="fetB"
                     /locus_tag="VBC74_000050"
     CDS             complement(52081..52854)
                     /gene="fetB"
                     /locus_tag="VBC74_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEO6941038.1"
                     /translation="MSGANSISLMSLLMASSLVLITLFFSYWQKLNLEKEVIISAIRA
                     VIQLLAVGFLLDYIFGYQNPIFTALLMLFMIINASYNAAKRGKGINKGFVISFIAIGS
                     GTIITLSVLIFSGILKFVPNQMIPVGGMIISNSMVAIGLCYKQLLSEFRSKQEEVETK
                     LALGADILPASIDIIRDVIKTGMVPTIDSAKTLGIVSLPGMMTGLILAGTSPIQAVKY
                     QMMVTFMLLATTSIASFVATYLAYKIFFNNRKQLVVTKD"
     gene            complement(52851..53507)
                     /locus_tag="VBC74_000051"
     CDS             complement(52851..53507)
                     /locus_tag="VBC74_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6941039.1"
                     /translation="MSYLTFKHVDFKTDDKLVLNDINFAIDEGDFVSIVGPSGSGKST
                     VLKLASGLMSPTAGHIFFDGKDLNQLEPIESRKMISYCFQTPHLFGNTVEDNISFPYH
                     IRHEKVDYRRVDDLFQRFEMDQSYLKQDVKKLSGGEKQRIALIRQLLFEPKVLLLDEV
                     TSALDNHNKAIVEKVIKSLHDKGITILWITHDEEQSRRFANKVLKVVNGSIEAMEVIK
                     "
     gene            53724..54362
                     /locus_tag="VBC74_000052"
     CDS             53724..54362
                     /locus_tag="VBC74_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEO6941040.1"
                     /translation="MFLIDFILHIDTHIYAMANTVGNWTYLLLFLVIFVETGAVIFPF
                     LPGDSLLFAAGALAANPKMSFNIVTFLIIFFIAAFIGDSCNFLIGRTFGYRFIKHPFF
                     RRFIKEKNIRDAELYFEKKGTAAIILGRYIPIIRTFVPFVAGISQLPPKVFIKRAFIA
                     ALSWSVIATGSGFLFGNIPFVKQHFSLIILGIVFVTLIPVLISGVKSYRNTK"
     gene            complement(54440..55225)
                     /locus_tag="VBC74_000053"
     CDS             complement(54440..55225)
                     /locus_tag="VBC74_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6941041.1"
                     /translation="MKRLHKLFITVIATLGMLGVMTFGLPTQPQNVTPIVHADVNSSV
                     DTSQEFQNNLKNAIGNLPFQYVNGIYELNNNQTNLNADVNVKAYVQNTIDNQQRLSTA
                     NAMLDRTIRQYQNRRDTTLPDANWKPLGWHQVATNDHYGHAVDKGHLIAYALAGNFKG
                     WDASVSNPQNVVTQTAHSNQSNQKINRGQNYYESLVRKAVDQNKRVRYRVTPLYRNDT
                     DLVPFAMHLEAKSQDGTLEFNVAIPNTQASYTMDYATGEITLN"
     gene            55486..56931
                     /locus_tag="VBC74_000054"
     CDS             55486..56931
                     /locus_tag="VBC74_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEO6941042.1"
                     /translation="MKKTIGIIGMGVSGLAVLLALSRKKEKELKQLAIFCFDDTVHFG
                     RGIPFQEDVDSALINSPINDISFDYRHMSDFMDWLEENYYDTNLTYVSRTLYGQYLSE
                     RTKLLLDKLPVTVIKTRVDKLNYLSDEQQWQVYIQDKLFPVTFDELHLACGALPVIEP
                     YHLEGKTDYIKDPYPIKYLAQENWKGKTVAIIGTGLAAIDVIKWLLLNTQSSVKAFSR
                     TNYFPSVRIVNDLPLTWHFFTNDNLKAFLSHPSKTFTIADFNDLMSGELEHLGFKNWK
                     NTTETFLSPGIDGLKLAFKYPKQLYLLQQLASRITVWLTDLWPLMSLSDKKEYKALYG
                     KAIINLRNPMPDESAKVILKAAQKGTFSLINNVKTITKNDEGFSIEKEKQNHISVDRV
                     INATGYQLNTKNWTKANPLLKSLINQEFCQIDDQGGITILTENAQVLSPKYGPMPGLY
                     AHGALVNGVVYQNNSTIKIQQMAERAFSDNK"
     gene            complement(56988..57659)
                     /locus_tag="VBC74_000055"
     CDS             complement(56988..57659)
                     /locus_tag="VBC74_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6941043.1"
                     /translation="MLELKNIAYRYKGNDNKTLENINYSFQSGVFYTILGNSGSGKTT
                     LLSLMAGLDSPTEGQVLFNKKDIKEAGYAQHRKKNIALVFQNYNLLDYLTPLENVQLV
                     KPTADKQLLLDLGLKEDMLTRNILRLSGGQQQRVAIARALVVGTPAILLDEPTGNLDF
                     DIARDITMRLKDFAHKEKRCVIMVTHSREIAHMADTALQLIGDNLKELSKESIAQSIL
                     QQRNL"
     gene            complement(57756..58076)
                     /locus_tag="VBC74_000056"
     CDS             complement(57756..58076)
                     /locus_tag="VBC74_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO6941044.1"
                     /translation="MIAIPAFALSVLISQFIGQKLADSLVYQASRTVRADFMKGFGNM
                     QLGADSDTDMFMQTIKTLNVQVKPEEVWLVILVGLAIVLFAIITTSLIIARYKPRGIL
                     SKLS"
     gene            complement(58085..58213)
                     /locus_tag="VBC74_000057"
     CDS             complement(58085..58213)
                     /locus_tag="VBC74_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000962057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO6941045.1"
                     /translation="MLVVSIILVMMALLFWIGGRTHETGILLAIGKSKAIIIVSTQ"
     gene            complement(58314..59108)
                     /locus_tag="VBC74_000058"
     CDS             complement(58314..59108)
                     /locus_tag="VBC74_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6941046.1"
                     /translation="MNLFKRSFLYVSRKKRKSITLFVCLWLVASTLISGIAVKNAGLT
                     AKKTFSRQTGSILHISSDSTDLVGDGYGSGEIPEKAIVNIASNPNVKRVNNNLMAYAG
                     LTSEKMVTRPNDKEQYKEQVLQVHGNSYSDTDPKYTAGMISLKGGRHITPKDQRVIIV
                     HQAFAKTNHLKVGDKLMFSKDPVRNSKDKTLVEATIVGIYKGKTVQKSNYPYEMLENT
                     AFSDTQLIKDLYGYSKGKAFYRQTTVYPKKNADIETLKSFIKKQSY"
     gene            complement(59118..60347)
                     /locus_tag="VBC74_000059"
     CDS             complement(59118..60347)
                     /locus_tag="VBC74_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6941047.1"
                     /translation="MNLWTLSLAYLKRQKMKTVTLFLVFLTIGTCLISLMSIQHSLEK
                     NILTKQGKSIYLTSKEKAYWPEQAYEALKKAKMVESVEASLSIDVGSRLKSVSPYNSS
                     KEENQVTLAGYQSTEDLRAFQTKALVLKKGSHLAADNTKQVLVPLKLAQKNHLSVGNK
                     LRLGKENVTIAGIYDANSAKSKNTFNPNIDNTLIAQATLVRKISKQKGYQTVAVRLSD
                     KRLVDTVIQNIKQWPLDFGKLDVQTAKEFYGDSYRNIETLHRLVGRIILIVSLVAMAI
                     LVVMLTFWINNRIKETGILLAIGKTKFEIIGHYLIEVLLVAGAAFTLSIIGGVFLGKT
                     FAAGLLSQVNGGVSSQIVQNSSLIIDRIDNLAVSVGVMDVFRLYAQGALICLFAVVLS
                     SYSILKLQPKQILSRMS"
     gene            complement(60471..61532)
                     /locus_tag="VBC74_000060"
     CDS             complement(60471..61532)
                     /locus_tag="VBC74_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6941048.1"
                     /translation="MNKITTLSTIALTLMLCVGCSANKDNQKTKTEDPKQENVTANPK
                     EDKATAAKRMKLTKELEKYNKGTESFISNKQYQKYIKTPIKEYGQKVEVKGKKMNVYT
                     VGEGKVPIVFIPGQGTVTAKHQYHNLISNLSKTHKVVVVEPFGSGLSDVIDQPRNLAN
                     ITSDIHEALQKVGITGKYVIASHSIGGVYALKYISTYPKEVLGLIGLDTSTPGMEGGK
                     QVDFAAPVLKELPKIPKVSDDINAQFFAIGHKILNNSNMKEEAKNSSNMINESANYKI
                     PKGIPAMYLLARESDLGLQLRSLDIPIKGSWQEQHKELSEDPKEVSIHTLDGTHQIYD
                     TNLKEVIDYTNQFMKTFEK"
     gene            complement(61567..62586)
                     /locus_tag="VBC74_000061"
     CDS             complement(61567..62586)
                     /locus_tag="VBC74_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO6941049.1"
                     /translation="MNKITKLSTVALALMLCVGCSANKDNQKTKTEQPKQEKSIAKQK
                     QEREAEKAMIAKGAIDLTKYKAKPIEDYGQKIDVGDGKKMNIYTMGQGKIPLVVFPGQ
                     AEISPRYAYKNLLDRLGKKYKIYTVEPLGYGLSDMTDKPRTIENITKEIHTALEKVGV
                     KNFYLMAHSLGGIYGLNYAKTYSNDVKGFIGMDTSTPNMEGDGTTGESSASTLKWAES
                     IPEVDKKTNEQYLSIGAKKSGNKTERDEDRRVSDNLHKMEKVKFPKGMPAKYFLANES
                     AEDIKMRKKIFPKLKSWEQQHIDLSEDPKDVSVETLDASHLIYHTQYKHMAKAIDEWI
                     QSHEK"
     gene            complement(62621..63652)
                     /locus_tag="VBC74_000062"
     CDS             complement(62621..63652)
                     /locus_tag="VBC74_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6941050.1"
                     /translation="MKNITKLSTVALSLLLCTACAASNTSTSKTQSHHPKQTKLTDKQ
                     KEEPKNKEAADQEMHPQGAVDLTKYKAKPVKDYGKKIDVGDGKKMNIYETGQGKIPIV
                     FIPGQAEISPRYAYKNLIERLSKKYKIYTVEPLGYGLSDIPTKPRTLENITKEIHTGL
                     NKIGVKNFYLAAHSLGGMYSLNYAKNYPEEVRGFIGMDTSTPWMEGEQKTKYDPESAK
                     WAMKLPDVDDKTNEQYLSIAKKINGNKSQRDEDERLGDNLGKLKNVKFPKGMPTKYFL
                     ADQSVEGIKLRQADFKGLKNWDQQHIDLSEDPKDASVEILKGSHNIFQTQYEHMAKAI
                     DGFIQSHQK"
     gene            complement(63684..63794)
                     /locus_tag="VBC74_000063"
     CDS             complement(63684..63794)
                     /locus_tag="VBC74_000063"
                     /inference="COORDINATES: protein motif:HMM:NF013590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941051.1"
                     /translation="MRANIPVRNFQEFYDAFGVKKGDSMYLKPEKRLTLW"
     gene            complement(63791..64609)
                     /locus_tag="VBC74_000064"
     CDS             complement(63791..64609)
                     /locus_tag="VBC74_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEO6941052.1"
                     /translation="MGDYYGKKYFGEAAKKDVEHMAKKIINVYKTRLKNNTWLSENTK
                     AMAIKKLDNMRLMIGYPEDYPDLYRQYQFDSKASFFENNDNYRKLSNKKTFEEFNQSN
                     QREHWQMSANAVNAYNDPNTNSIVFPAAIFQSPLYDKTKTVSQNYGAIGAIIGHEISH
                     SFDINGMKYDEKGNLHDWWTKEDLKHYKKKTQAMIDQWDGLKADGGKVDGKLTLAENI
                     ADNGGVMASLEALKTEKIQTIKNFLNHGQVFGVKKQPKNKVSPQFSQMFMHHMN"
     gene            complement(64630..65715)
                     /locus_tag="VBC74_000065"
     CDS             complement(64630..65715)
                     /locus_tag="VBC74_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M13"
                     /protein_id="HEO6941053.1"
                     /translation="MQRKSYLKSMSVLTLTACLISGYVVKDIAMLHAVSASEKKANNV
                     SPRENLYRAVNDNWLANTKLKQGQTSVNSFSEIEDKLKQLLVSDMAKMASGKIETTND
                     EQKKMVAYYKQGMDFKTRDKNGLKPLKPVLQKLEAVSSMKDFQSLAHDFVMSGFVLPF
                     GLTVETNARDNSQKQLVLRQAPALLESPDQYKKGNKEGEAKLSAYRTSAMALLKQAGK
                     SNIEDRKLVKQAIAFDRLLSEKTQVDQSKITAESETAAGRYNPESMETVHNYAKEFDF
                     KELIEKLVGPTNKAVNVEDKTYFKQVNDVINSKQLANMKAWMMISMLVDQSDFLGEQN
                     RQAASAFKNVASGLTQIESKEKMLTPN"
     gene            complement(65899..69216)
                     /locus_tag="VBC74_000066"
                     /pseudo
     CDS             complement(65899..69216)
                     /locus_tag="VBC74_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060267.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(69262..71919)
                     /locus_tag="VBC74_000067"
     CDS             complement(69262..71919)
                     /locus_tag="VBC74_000067"
                     /inference="COORDINATES: protein
                     motif:HMM:NF017709.1,HMM:NF018035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO6941054.1"
                     /translation="MTKPQGAVAEKATPAVPKPQKVRVIVEFNKESILDYATEQKKTV
                     AQLNQADVEKKLQSIKQEQDKVLKNIEKSVHFDSSKVKRYDAIINGVALDIQAQEIEK
                     LKTIADVRRVYVSQEYVQTKPLLSSSGQLIGLPEVWNNSQYKGEGTVVAVIDSGVDFK
                     HQALKIKEPNRAKYNKTSIEKLIHEKNLKGKFYSEKVPYGYNYYDYNDNLKDSYGVMH
                     GMHVTGIVGANDDNQKLYGVAPNAQILAMKVFSDDQQNPTTFTDVWLKALDDAILLKA
                     DVVNMSLGTPAGFVHEGKDYPELEVIARACKAGIVIAVAAGNEGNITDGNTYGVKPLA
                     ENYDTALIANPALDDNTLAVASMENLKKHAHVLKFKDKKSGTEVTEVINLHVAPNASK
                     TIIGLAVDLGAEAPSELSKHFDLSGKIAMLEIPEDNKSNGFLEKVQAITKLNPAAILL
                     YNNAKVKDDLGSQLLVESEAAKFNIARITRSTYNNIKNNSNKIITILTERQAIDNSLA
                     GQLSSYSSWGPTPDLRLKPEITAPGGHIFSTVEDNQYADKSGTSMAAPQVAGAAAVLK
                     QYITDKKIPVDNAADFIKLLLMNTAQPIINKQSKDGKTPYFVRQQGSGAMNLAKALVT
                     TVVATVTGTNDNNADGKLELRELKEKKFKARILLRNFGKTNKTYIISSEAIADPVDEK
                     GFRTQNSEHLVSKKADAVTRKVTVEAGKTLAVDLDVDYSDAEALTRNNFLEGYLNLKD
                     TEGVADLHLPFLGFYGSWTEQKAIDAFEGISEIGNGDKKRRVQFYVNKETNKTSSTFT
                     TNGMLSLPIYNNTVFFSPNSPFYDKAGVRIAALRNMEYVQYSIIDPDTNKEVRVLGRS
                     HDVRKLYRLDYRNSFAMMPDSIWDGKIKD"
     gene            complement(71971..72486)
                     /locus_tag="VBC74_000068"
     CDS             complement(71971..72486)
                     /locus_tag="VBC74_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941055.1"
                     /translation="MTFYKKTDKFGFRKSKVCRSLCGALLGTVAVVSLATASTEIHAD
                     EATTSPTTVTKVPQPVQADTTALNTSKTHSTQATTTPVEAKENKVVKSETVQSESRVM
                     PRDKVVERPETVKASVNSDVSQPITTTPPTINEKTVEIPNLAQDTKKVAPKVTVTPEV
                     VNQPKQLPKYE"
     gene            complement(72520..72861)
                     /locus_tag="VBC74_000069"
     CDS             complement(72520..72861)
                     /locus_tag="VBC74_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941056.1"
                     /translation="MSEFLSAKPIGHNDSSNTILRIIENTKYIHHYDGKVVVEIPPQK
                     EPIKVFFMDSRLHYLEFSDICRYVTVVLKNSTVDNITNLGLDNHIVYDNGDDMVDPLP
                     SIQHGLFLNDD"
     gene            complement(73204..73779)
                     /locus_tag="VBC74_000070"
     CDS             complement(73204..73779)
                     /locus_tag="VBC74_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylK protein"
                     /protein_id="HEO6941057.1"
                     /translation="MNPLSEKDKVNYIMIYKNRQSTLENNLSGDRLMRYQQANRQVSK
                     DKLLTGSYFVYQCLKKLGFTNKICQESFSSVSSYLIGLPKGKISYSNSGDYHILTYAP
                     SGSTGVDIEKYKDRSEQTYQNYLGESVSSDMDSKLLFYKAWLQKEISYKCGKSIDITY
                     QTMIDGYIYGYAFDNTFKVEAVYLKELLPDN"
     gene            complement(73748..74959)
                     /locus_tag="VBC74_000071"
     CDS             complement(73748..74959)
                     /locus_tag="VBC74_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylJ protein"
                     /protein_id="HEO6941058.1"
                     /translation="MSKFLFAPFMMNLGESQRLSKLANCLYEQGHDIHILGDNYYPFL
                     FNNDAYRYHHCVEDKSVYNSKRYDAFFSLSTDFNFLSEEEIERICSIERDLLRQEKFD
                     AVLTGYRLSIVTSCRLESIPLIWIISGATHISEIVENSEGILPNGKISKASKPQTKDF
                     IKRVITTYSTNVKTWNNYIKKYGGKPFNNALELFTGDLNLVTDYSLFYEFDKDSSYKT
                     IGPILIDNVGFSKCSQINQDNKTVLLSFGTSFKRDWVESFLKTLPRHYHYLLTSCGEA
                     INIPGSYIEIVDFIDFKTIDKEIYFAIIHGGQGTVYAMAAQGIPFIGIPFFNEQFWNI
                     KKFSKEKCAYLVKKPDVREIRKAISCLERDYDIYKSYMIRLSQGIKEEAEASLQKAEA
                     EIESFIRKRQS"
     gene            complement(74964..77159)
                     /locus_tag="VBC74_000072"
     CDS             complement(74964..77159)
                     /locus_tag="VBC74_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEO6941059.1"
                     /translation="MSVYVSGIGIISSLGKNYSEHKQHLFDLKEGISKHLYKNHDSIL
                     ESYTGSITSDPEVPEQYKDETRNFKFAFTAFEEALASSGVNLKAYHNIAVCLGTSLGG
                     KSAGQNALYQFEEGERQVDASLLEKASVYHIADELMAYHDIVGASYVISTACSASNNA
                     VILGTQLLQDGDCDLAICGGCDELSDISLAGFTSLGAINTEMACQPYSSGKGINLGEG
                     AGFVVLVKDQSLAKYGKIIGGLITSDGYHITAPKPTGEGAAQIAKQLVTQAGIDYSEI
                     DYINGHGTGTQANDKMEKNMYGKFFPTTTLISSTKGQTGHTLGAAGIIELINCLAAIE
                     EQTVPATKNEIGIEGFPENFVYHQKREYPIRNALNFSFAFGGNNSGVLLSSLDSPLET
                     LPARENLKMAILSSVASISKNESLSITYEKVASNFNDFEALRFKGARPPKTVNPAQFR
                     KMDDFSKMVAVTTAQALIESNINLKKQDTSKVGIVFTTLSGPVEVVEGIEKQITTEGY
                     AHVSASRFPFTVMNAAAGMLSIIFKITGPLSVISTNSGALDGIQYAKEMMRNDNLDYV
                     ILVSANQWTDMSFMWWQQLNYDSQMFVGSDYCSAQVLSRQALDNSPIILGSKQLKYSH
                     KTFTDVMTIFDAALQNLLSDLGLTIKDIKGFVWNERKKAVSSDYDFLANLSEYYNMPN
                     LASGQFGFSSNGAGEELDYTVNESIEKGYYLVLSYSIFGGISFAIIEKR"
     gene            complement(77156..78109)
                     /locus_tag="VBC74_000073"
     CDS             complement(77156..78109)
                     /locus_tag="VBC74_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEO6941060.1"
                     /translation="MNTIYNTLRTDKGYKVYEGYLYEITGEECEEALDLVIPKNIVFA
                     DTDTCGYTFLLNEDGTVYDDVTFYKFDDKYWLASHKALDSYLDNINFDYTVTDISDEY
                     KMLQIEGRYSGEIAQSFYEYDISTLNFRTLIEMTYKGEKGYLARFGFSGEFGYQFFLP
                     SSIFATFVSDVCEGIAEYGDELDRYLRFEVGQPITDIYQQEEYSLYEIGYSWNLDFTK
                     EEFRGRDSLLEHIRSATVKSVGFSTKEKLASGTPVLFDDQIVGKIFWIADEKDSSENY
                     LGLMIVNQTYAHSGVTFVTEDGQILKTQSSPYCIPESWNKE"
     gene            complement(78106..80109)
                     /locus_tag="VBC74_000074"
     CDS             complement(78106..80109)
                     /locus_tag="VBC74_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylE protein"
                     /protein_id="HEO6941061.1"
                     /translation="MKDDNKLKISEASLEDYSEVVHLFNRNHVYQFPDGRPLTVDDLD
                     LTLKVKEVTHLFLLKNHGVLIGTSAFFKFITYGCLDWNSSFSGFLLIDSKSRSGQAIT
                     YLYKTILKKITKLKFSNIYTEISNYNKPSLALSKLNGFKEYDKTYEDILHCRSLRSHL
                     PKILNTFRISNYYGKTYDISTFQIMEEIENPLEEETEIRTKVSDEEILFKAEDSASLP
                     YYLKMSLFQMEIAKLDNRYVLQVDFLSEQVKRVRVKTGKYHLANLTRAHPSLTLSRFA
                     NYYYIQATVETLYGNIDVQLERRKKHYRDATICLKRTFQGYDLLISPNGSLIFEKQKR
                     KILEDSFLIFSQPLDKKLVVKEEENHITIKCFYQGALIEKIMTFTSDEEITCVYKCNQ
                     KAKEMFPKLLKQTFKLHCQEQLIRDSEGYLVNVPGSYPIEHDDFLRADKFEDRQFHYY
                     LPNEDKMISYSPPGKASNQMQFRPLCLIDTDSLSFPLTYHFRISQASSEEALINLKKQ
                     PIWDSNYQASATDLLKHISNLTLEEEKDYGIKRMIANRKHYPSHKLVLAYNQIVLPKN
                     EIPRDSELYSISFDYRIRGKFVQIRQGEHVKYDNKSYVLENSQQLVLYVASDDKYILI
                     SAKNGIFYSYKENNHLKIRCMFKRNSPYATNVSITEYRKCEEK"
     gene            complement(80106..80984)
                     /locus_tag="VBC74_000075"
     CDS             complement(80106..80984)
                     /locus_tag="VBC74_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6941062.1"
                     /translation="MFELISRNRKVYTRDRLAFFMSFLSVIILILVYQIFLGKIQLDA
                     IKAALRSNTVSSDTIHMVNYWLIAGLTTIISMTSTLGAFGVMVSDKEKKLNEDFKVSP
                     ISNIKIELSYAIFAVLFGIVMTMFSCIFAIGIFNGFNSLLEYSAMDYLTILGIVSLGT
                     VLSAAMILPILAFIHTSSAFTTLSTIVGTFIGFISGVYLSIGSVGKALQQVMTWFPLT
                     QINSLLKQVLMKGSIAKVFDKANEATVSNYKESYGVVLRNADGERLSNHFMLIYIIAL
                     ILILLAIHFIIKKVKK"
     gene            complement(80977..81906)
                     /locus_tag="VBC74_000076"
     CDS             complement(80977..81906)
                     /locus_tag="VBC74_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6941063.1"
                     /translation="MEIKLKNIGKKYGSFEALRDINLIFEEGKFYGLLGPNGAGKTTL
                     FNLLIQNFKQTSGDIKWEVDGKPLSIKDFYRHIGIVFQSNRLDDNLTVEENLISRGAL
                     YGLSKSQVRNRLKDLQTYLDITAIKKQKYGSLSGGQKRKVDIARALLPQPSLLLLDEP
                     TTGLDPQSRRDLWDAIAQLNQQSQMTVVLITHYLEEMSACDVLNVLIEGNIYYSGDIK
                     SFIEQHSTTNLNVVLKPEKSLDQLSIADFVNKCQVLSEREIVFKDISVEEMMQVISDN
                     QDKSIIETFNVEYSNLEAAYLNLLREKERKNNV"
     gene            complement(81896..82372)
                     /gene="fabZ"
                     /locus_tag="VBC74_000077"
     CDS             complement(81896..82372)
                     /gene="fabZ"
                     /locus_tag="VBC74_000077"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO6941064.1"
                     /translation="MTKLSSEEIIKMLPHRYPFLMVDRVQSYKKGIIECRKNITINEP
                     YFQGHFPQRPIVPGVLMIEMAAQSAALLYILDALDGSLPSLDELTAEKIAEKVGYLAS
                     VKNFKFKKIVTPGDQLNITCKSQSKLGHLLEIQVVIRDENKKEVASGRMLVSENGN"
     gene            complement(82356..82661)
                     /locus_tag="VBC74_000078"
     CDS             complement(82356..82661)
                     /locus_tag="VBC74_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO6941065.1"
                     /translation="MINWAEERKIIKTRQEVVDEIKGILIDSLMLDIDKDLIMNDQPL
                     FGRGLELDSIDALELSIGISTTFGVELNDDDISILSSVNRLADFVIENSEDIDDQAK"
     gene            complement(82654..83376)
                     /gene="fabG"
                     /locus_tag="VBC74_000079"
     CDS             complement(82654..83376)
                     /gene="fabG"
                     /locus_tag="VBC74_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEO6941066.1"
                     /translation="MKVAIVTGGTRGIGRAITKELYKEGYKVIAIYNSNDAKARALQE
                     ELPKLDVYKCNISDAKAVQKLVTKIFREYGGIDCLVNNAGIVRDGFFLMMSKEKWMDV
                     ININIMGLVNMSKAVLKIMKAKRIQGKVINISSTSGIAGQIGQANYSATKGAIISITK
                     TLAKEFASDGITINCVSPGFIETDMTNELQNKEELKEHLIPLKRFGQPEEVAWLVSFL
                     ASEKANYITGKNIVIDGGMIND"
     gene            complement(83373..>83736)
                     /locus_tag="VBC74_000080"
     CDS             complement(83373..>83736)
                     /locus_tag="VBC74_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CylD"
                     /protein_id="HEO6941067.1"
                     /translation="AMDQVNRSFFDDVMTNKGYFYKLKQLGVKAPYHTDFLNESKEQY
                     KQLIESLDISQNDDYDYIFHCDQLVDEILYQWTNLFNWRAIKETIIEKDAEIIDISPN
                     RFISSQLQKMKKRGRKQL"
BASE COUNT        25866 a        16353 c        13177 g        28340 t
ORIGIN      
        1 acaagtgaag taggattact tcgattagtt gttcacccct tcgttccatt tccattacag
       61 aaacttcatc actactatgg gttcggctga cttcttgtcg ttcgttgtta ctaggtttta
      121 cccttgacag gacctcacgg gataagtcgg acatctttcc tcgtttactc tcgtgattta
      181 cgtatatagg ttacgactac ctatttggac tttagagttt atagccccct catccgctat
      241 atacgccttc atatcacgtt tctgttcgta gagccatgat ttcgctatcc cttcctctcc
      301 ccgctacctc acgatagtca agcttgggag tcgctattgg gttcaccggt agcgggtgcc
      361 cacagaggac ttgcacctca gatgtacgac atgcccgtcg tactataaaa agaactttct
      421 aaatgaaagt tcttttttct tataaaattc cttccagaag gccacgcatg aaatcttctg
      481 aattaaattc accaatatca tcgatttttt cgccgaaacc aatgaattta acaggaatat
      541 ctagttcttg acgaatagcc agtaccacac cacccttagc agtaccatca attttagtca
      601 gaataaggcc agttaatggt gtaattttcg agaactcttt agcttgactt aaagcatttt
      661 gtcctgtaga tgcatctaaa gcaagcaaag tttcgtgggg agcatctggt acgacacgct
      721 taataatacg tccaattttt tccaactctg ccataaggtt ttctttattt tgcaaacgtc
      781 ctgctgtatc aattaacaag acatcaacgc cttgagcaac ggctttttcc atgccatcaa
      841 agacaacgct agccggatct gctttttttt ctccagtgac aactggtaca tcaacacggc
      901 gtccccattc tactagttga gccacagcac ctgcacggaa agtatcagct gctactaaca
      961 taactttttt cccttgagac ttgtattgat gagctaactt acctatagat gtcgtttttc
     1021 caacaccatt aacacctaca aaaagcatta cggttaaacc ttcttggaaa ttgatagcct
     1081 cattataaat accatctttt tcgtagattt caactaattt ttcaacgata acacgtttta
     1141 aatcctctga tttcttggca ttctctaatt ttgcttcata acgaaggtct tcagttaact
     1201 gtgttgcaac atttacccca acgtcagata agattaacat ttcttctaat tcttcaaaga
     1261 actcttcgtc aacacgacgg aagttagaaa ggaaagcatt aaggcgtgca ctaaaacctg
     1321 tgcgtgtttt tttcaggcta cgattatatt tttcctcttg actctcagct tttatagtgt
     1381 ctgcctgtga ttgcgataaa acttcctgtg cctcagaaag ttctgactca tctgttgaca
     1441 gcgaattaga cgagatttct ttctcaattg ctttacgttt agcatagtaa tctgctaaaa
     1501 aagcatcagc cgcagatgta tcctctgact ctgtttgtac ctcagttttt ccactagaca
     1561 tctcttcttg aaggggagtt tctgaaaact gctctgaagc ctgactatca cttgattgct
     1621 cctcatcact aaaagatgcc tcagatttag cagaaacatt gtcagaaaga gtcgtggaat
     1681 caggaactac atttcctacc tcagaagtat ctgccacctt tgtcttagcc ctatcttcta
     1741 gttctaaaaa actatcgtct tcttttacag gaatttcaga ggtatcttct gactctaaag
     1801 gatgaatttc agaggtaact ggttgatgtg attctgacaa tgctgtatct ctttctaatt
     1861 ctaagaaaga atcatcttct gcgacaggga cttcagaggt tcgattcaaa gttgactcct
     1921 tcgaaaagtt gctgctttcc tctttatcaa taactgaatc ttcattttct aaaacaacag
     1981 attctgatgc ttcaatttct agttctttat cttttttctt atgtccaaat aggcggtcaa
     2041 ataatcccat taattttcct cacttaaaat atatttccca actgcttcgg caactccaga
     2101 ctcatcgtta ttacaaatag ttgtcgcttt tgctgttgcc ttagcttctg gaataccatt
     2161 agccattgca actcctaagc ctgcccactc tagcattgat aaatcatttg cttcatctcc
     2221 cattgccata acttgactac tatctaaccc aaggtgtttt gtcagtagtt ctaagccgac
     2281 tgctttatga acacctttag gcatcaattc taaaatgata tcacgtgatt taaacatttc
     2341 atagtcaact ttaagtctat caggaagttt agcaatttgt tgatcaagat atgtagcatc
     2401 aataacacta acaattttat tataaactac atcctttggc acttgctcta aatcatcaac
     2461 ttctataaat tctaagagtg ggttggctaa atgatattga gaatggtgtc ccttgttagc
     2521 aatactatat accgttccct cacttaagat atctgttggc aaacccactt gatacaactc
     2581 ctcatggata tcttccactt cttgtcgtgt cattgcagtt ttagctaaga ttttccctgt
     2641 attttgttga actaaaccac cgttaaaagt aatgctgtaa tcttcatcag atactaactc
     2701 taggtcctct aacaaattac caattgcttt taaaggacgc cctgttgtaa taacaacttt
     2761 tatacctttt tctctagctg cttttaaggc cactttattt tcttctgaaa ctttcttatc
     2821 tgttgtaaat aaagtgccat ctaaatctaa ggcaagtatt ttaatatcca acattaatcc
     2881 accatacttt ctaaataagc cattacagct tgatcattgt gatgacctat aatacaatct
     2941 gctatctctt taacttctgg tctagcattt tctgttgcga tagcttttcc tgaccactct
     3001 agcatttcta aatcattaat attgtcacca aaagataaga cttcttctgc tctaataccg
     3061 tattgttcac agaggtgctc taaaccattt cgtttattaa ctgagcttag aataatatca
     3121 atggatttaa aacctgttgt cactgctgtg gcatacggta tagcttgatt tacccactcc
     3181 tcagcttgtc taacagtctc ttcagtaaag ttagccgtta ctttgaaaac aatatcatca
     3241 acatcttcaa agtgagaaac tttttgcaaa ttatcataat aatgagtgat gaattcaata
     3301 taatctggat tggcatcact tagaatatat gcaccatcct tgccagataa gacatattca
     3361 ttattctcca tataagggct cttactcaaa tggtcaataa tatctaagta ttgttctcga
     3421 ctcaagtgct gttcatatgc caagcgatta aataagactg ctgcacttcc gttttctgct
     3481 ataaaagcca tctgatcacg aaagtcagca aataattgtt caagtgataa taaggaacgc
     3541 ccacttgctg ccgtaaagac aattccttgt tctttaaatt tttttagcac atttgctaaa
     3601 cgttttttat cataggtacc gttttcatct aagaatgtac cgtccatatc ggttgctact
     3661 aattttatca tggaatctcc taactaaagg gtttacattt actattgtaa cataaaataa
     3721 ggctgaaaat ccaaaaaaca gaagggatcc ccttctgttt tctaattggt catttcttga
     3781 gcttctttta attttacaga aacaattttg gaaacgcctg attcctgcat cgttacccca
     3841 tagatactat cagcagcaga catagtccct tttcggtgag taacaacgat aaattgactt
     3901 gatttatcaa atcgattaag ataatcgcca aatcgcttaa catttgcttc atctaaagcg
     3961 gcctcaactt catccaagat gacaaatgga attgtcttaa cacggataat ggcaaataat
     4021 aatgctaacg ctgataatgc tttttcacca cctgacatca agtttaaaga ctggattttt
     4081 ttaccaggag gttgaaccga aatatcaacc ccagctgata ataaatcacc ttctgtcaga
     4141 ataaggttcg cagaacctcc accaaacatc tgtacaaagg tttctttgaa actatgacga
     4201 atggcttcaa atgtcgattt aaaacgagtc ttaacttcat catccatatc tgtaatcgtc
     4261 tctagtaata aatttttggc atgaactaag tcgtctcgct gtgtgttaag gaaggtcaat
     4321 cgttcatgaa cttcttcaaa ttgagcaatt gcatcaatat taacaggtcc taaagcttta
     4381 attttagctt gtagagattt caattgttca cgcgccatca aaatatcttc caagacattt
     4441 gcttttacct tcgcttcatc caatgtcatc tgatagtctt ctgataattg acgtgagaat
     4501 atcatcaatt gttgagataa ttgttcacat tgtgactcta attgagcttg ctgacgaata
     4561 aggctttcat ttttttgtcc ttctttagcc agagaagcag ctaaatcatc taaagcagct
     4621 tcacaatcct ccaattcaaa acggagactc acaagctttt cttcgtcatt ttctcttctc
     4681 ttatttgctt gtagcaactg tttttctata cgtggaagct cttctttgtc taattgactg
     4741 tcctgatggc ttagtaacgt ttgtaaatta ctaatgtcac gttttatttc agacaaggta
     4801 atctctagac gtgtacagtt ggcacgttca aatttctgct cgtttagcaa atcacgttct
     4861 tctagctgtg cctctgacaa taatgttgtt agattattga ctttttcttg aattgaatct
     4921 ttatcttcct taatttgagc aattgaagtt gtcaactctt cttttttaat agcaaattgt
     4981 tcaagttctt tttctaaacg atttttttca ttttcagaag tagtttgttc caacgccccg
     5041 ctgcttaatt gcaagccatt atatagttta tttaaatcag caagcttttc tgatagttgt
     5101 tgatactcta tatcagctct ttgttcctca agacgagctt gttctccatc attcttaagt
     5161 tgtgctaagg tttcttgttt ctctttgagt tgctctaaca atgcggcaac ttctttttct
     5221 tgaattaact gtttactttg tgcctgtttc aattcttttt ttagattatc caattcaggc
     5281 ttgataaaaa cagtattatt ttgacgattt gctccccctg agtacgaccc tccagggcgt
     5341 aactctgttc catctaaggt tactaaacgt acttgataat tcaattgtct tgcagctaca
     5401 ttagcattat caaccgtatc aaaaatagca gttaacccta agttattttt aaaaatgttt
     5461 gacaagcgtt gatcataggt taccaattca ctagctattc ctaaaaaccc ttgagaactt
     5521 tgtaattttg agagataatg ttgtgctaat tctcttggtt ttattgtagt taaaggcaga
     5581 aatgtcgctc taccttggcg gtttttcttt aaaaaagcaa ttgaacgttt ggcagcactc
     5641 tcgtcttcaa caatgatgtg ttggctgcta cctcccaaag ctatctccaa tgctgtttgg
     5701 taatgtttgt caaagctaag atgctcacta acagcaccta tgatacctcc aagctggtcc
     5761 ttagcctgta gaactgactt cactccagca tagaaattac tatgattttt caaaatagat
     5821 tctaaactcg aaatacgtgc ttgctttgat ttgatttcat ctaaatgatc aaataaaatt
     5881 gactgctgat tcttatactc accttctaga ttttgcaagg tttgacctaa atcttggtag
     5941 tgagataata actgacgaac attcttcttt gcagcctcaa atcgttccaa tgctaccttt
     6001 gcgttacttt ttaatacttc taaatttttg ctaacttctt gtatttcttg agatttagct
     6061 tgagaagctt gtttttgatt ctctatatca gctagtaaag ctgttaaagc gtttgaagtg
     6121 tcggcttctt cttgcatcaa ggtaacaaag tcttctcgaa gtttttcaat aacctgatcc
     6181 ggattagttg aaaaacgctc taaggaactt tctatactaa caatttctga tcttatttgg
     6241 gctatttttt ccgacagagt agttaactct atattttttt gggtaatctg gtcattacaa
     6301 tctttagctt taacttctaa ctctgctaag cgttgacctg cctcttcttt cttttcagcc
     6361 ttttggcttg actctaaacg aatcagatca atttggcgtt ccaaatctga ctttaacttt
     6421 gtaacgtcta aaagagctaa ctgtttaacc tcaatttctt cagataaatg gtgacgtttc
     6481 tgttttaacg attgattttc atcttcaagg gactgacgtt gttggtaata agttgctaac
     6541 tcttctctta ctgtcaatag cttttcctca acagtagtta aatctgattg atgttgtaaa
     6601 atatcttcaa tcaaaataga taagtggagt ccttgtcgtt cctcatctaa tactaaaaaa
     6661 cgcttagcaa tacttgcttg tttttctaat ggttggactt gcatgtccaa ttcataaata
     6721 atatcctcta agcgatctaa gttcccttga gtttgttcta atttggattg tgtttctttc
     6781 ttacgtgttt tgtatttcag aacaccagcg gcttcttcaa atatggcacg gcgctcttct
     6841 ggtttactat taaaaatagc ttcaacgcgt ccttgggaaa taatagaaaa agaatctctc
     6901 cctaatccag tatccataaa taaatcatga atatcacgta agcgaacttt gcgtccatct
     6961 attaaatatt cactatctcc gtttcgaaaa atacgacgtt caactcgaac ttcgtcagct
     7021 atattttcga taaagtggtc gctgttatct aaagtaacac tcacttgcgc ataattaagg
     7081 ggcttacgat tttctgtacc agcaaatatg acgtctggca tcttacctcc tcgtaaactt
     7141 tttgcgcttg attctcccaa tgcccaacgt aaactctctg tgatattact tttacctgat
     7201 ccatttggac ctacaacagc agtaactcct tgatcaaatt ctaccttagt tttatccgca
     7261 aaggacttaa agccttgcat ttcaatttct tttaaaaaca tgatttcctc attgtaacgt
     7321 tgctaaagca tttttggcag cgtcttgttc agcagctttc tttgatcgtc caattccttg
     7381 gctcaaattt tcttggttga cagatacagt cacctcaaat tcctttgcat gtgctggacc
     7441 actttcatta gtaacttgat aatctatttt tacatcccca tgagattgaa gtaattcttg
     7501 caagcttgtc ttgtaatctt taacacgctc ataggtccct ttttcaacgt gagggatcat
     7561 taccttgtta acaaaggcat gaaccacttc aacaccttta tccaataata aagctcctaa
     7621 aaaagcctca aataaatctc ctaaaatagt gtcacgatta cgtccaccag atttttcttc
     7681 tccttttcca agtttaatat agtgatcaaa gccacataga cgtgaaaaac ctgcaagact
     7741 ttcctcacga acaatcattg aacgtaattt tgataaatca ccttctgcct tttgagggta
     7801 ctttgtaaaa agatactgtg aaataagtaa ttgtagaaca gcgtctccta aaaattccaa
     7861 acgttcatta tgtgaaatgt ttaggaggcg atgctcattt gcgtatgatg tatgggtaaa
     7921 agctgtatct aataattctt gattagcaaa aacaatacca tagtcttttt ccagctttga
     7981 tcttaattct ttcatttttt ctccttgatt tcaatcatag ataaaatatt aaacaaatac
     8041 cattatacca cagttgacaa ataatcaaac attttggaag ttaaacttat cttccaaaga
     8101 aaacgtttcc ttaattacat atttgaatat tcaacaaaag actaggatac acacctagtc
     8161 tcttgttaac tattcatcaa ttttatcagg tactacataa aaaagcacat aagttcctac
     8221 aatagttcca atcaataatc ccagtttgag ccacattaga ggagcaaaat atattgaaat
     8281 ccccatcaag atataaattt gccaaatcat tgacttttta cgcgctgggg caatacttct
     8341 gctttcaaca aaatctgcag cgtagtattt ataaacttta gtttgacgca accaaatatc
     8401 aaatttttca gagcttctcg aaaagcaaaa agctgaaagc aatactaatg gagtcgttgg
     8461 aactactggc aagacaatac ctgcaatgcc tatccctaaa ctaataaatc ctaaactaat
     8521 gtaaaaaatc tttttcaatc ttaaatcctc gtcagtgggg tagctgaatc aggtgaagta
     8581 tcgtgaacag aaaaatctgt gcccacacca aagtcagctc tcattaaatt gttttccata
     8641 gtcatgtgag caagttcctt aatattattt tccttactca agtgtcctaa atagatgtgt
     8701 ttagtacggt tacctatagt gcgaatcatg gtttctgaac catcttcatt agataaatgt
     8761 cctttatctg ataaaatacg ttgcttaagt gtccaaggat aagaacccga acgtagaatc
     8821 tcaatgtcgt gatttgactc gatcaaataa ccatctgcat tctcaatcaa gcttgccatt
     8881 cggtcgctaa catatcctgt atctgtcaac atcacaaagc tcttatcatc tttcatcata
     8941 cggtaaaatt ggggatccac agcatcatga cttacaccaa agctctcaat atctaaatcc
     9001 ccgaaagtta aggttttacc gcgtccaaaa acatgctttt gactaacatc aactttgcct
     9061 aacatgttac gctcatccat aactttccac gtctgctcat tggcataaat atcaaggtga
     9121 tatttcctag ccaatacgcc aacaccttta atgtggtcag aatgttcatg ggtaactagt
     9181 atagcatcta aatcttctgg tttacgatta atttctgcaa gaaggctagt cactttcttg
     9241 cctgttaaac cagcgtcaat taaaagacgt ttttggggtg tctcaatata aaaacaattt
     9301 ccagttgaac ctgaagctaa gatactatat ttaaatccta tttctgacat gttttaatct
     9361 tcaacgtcct cccattcttc ataagttaca gcatctttat cataaggcaa gacgattgta
     9421 aaagtagacc ctttaccata ctcactctta gcccaaataa atcccttatg ctgcttaaca
     9481 atttcttttg caattgacaa accaagtcca gtcccaccct gttgacgact tctcgcctta
     9541 tcaacacgat aaaaacgatc aaaaatgaga ggtaaatctt ttttgggaat acctaagcct
     9601 tggtctgata ttgataaaat catctgcgtt ttagttgtgc gtagattaac tgtaatctta
     9661 ccaccatctg gtgaatactt gactgcatta tttaaaatgt tatcaatcac ttgagtcatc
     9721 ttatctgtat caatttccac ccaaattgac ttaagaggat aatctctgac aatttcataa
     9781 acttttcctg tgactgtttt ttgatttcta atctgatcaa atcgattcaa aattgaggtc
     9841 atgaaagctg taaaattcgt catttcaaca tctaaatgcg ttacttcatt atcaatgcgc
     9901 gataaactta ataaatctga tatcatacgc atcatacgat ttgtttcatc tagagatacc
     9961 ttaatgaagc ttggagcaac ttcttcattg agcgctccct catctaaagc ttctaaataa
    10021 gattttacag atgtcaaagg tgtacgaagt tcatgagaaa cattagatac aaaaagtctt
    10081 ctttcacgct cttctttctc ttgctcagta gcatcgtgtg atactgcaac taaaccggaa
    10141 ataaaaccac tttcacgacg gttcaaagca aatcgaatcc gtaaggtaac aaattcacca
    10201 ttttcatcac gtctattaag aactacctct ggcgtcttag agactaaatc ttggaacgaa
    10261 tatggacttc ccgaccctaa catatcaaca atattcattg ataaagcttc atcatatgct
    10321 agattgaact gctgttgggc tgtctcatta atcataacga tttttcctga acgatctgta
    10381 gccagcacac cgtcagtcat ataagttaag atactcgcta agcgattctt ttcttgttct
    10441 aagttatcat gtgccattcg gaagacatca gataaatcat ttagactttc ccctaattct
    10501 accaaatctg catcaccttg aattagaata ctatctgaat aatttcctgc tatcaaatct
    10561 ctgactttcc aattcaaaag ccggatatct ttagacattt taaaatctct aaccgctaga
    10621 taaacaaaat agacagcaac aaaaactagt aaaaaaagta aggctagctc aaacgaccta
    10681 atatttgcag cactattatt cataagactt catgtaataa cctacaccac gtcgagtcaa
    10741 aatatattct ggacgaccag gagtatcttc aatcttttca cgtaaacgtc tcaccgtcac
    10801 atcaactgta cggacatctc caaaataatc atatccccaa accgtttcca ataaatgttc
    10861 gcgtgtcatg acttgtccta tatgtgtcgc taaatggtga agtaattcaa attctcggtg
    10921 cgttaattca atttcttcgc cacgtttctt agcaataaag gcgtctggta aaatttgcaa
    10981 ctcaccaata gtaatatctg aagaagcatt ttgagcactt tcttctgcta cagctgtttc
    11041 aatattttca gtacgtcgta aatgtgcttt gacccgtgcc aaaagttcac gatttgaaaa
    11101 aggtttagta acataatcat ctgcaccgat ttctaaacca atgactttgt caaactcact
    11161 gtcttttgcg gaaagcataa taataggaat atggctggtt ttacgaactt ccttagccac
    11221 ttctaaacca tctagttcag ggagcattaa atccaaaatg attaaatctg gttgaaattc
    11281 tgcatattgt actaaagctt cacggccatc gaaagctgtc gccgtttcat atccttcttt
    11341 ggttaaatta aatttgataa tatctgaaat tggtttttca tcatcaacga taagtatttt
    11401 tttcatattt tacctctatt actttctggt ctcccacttc catccataaa ctgaaaaata
    11461 aaagtgaccg aaaatatgat aatatgggcc taaagcacca ctattatcac taattataac
    11521 agatttccta gttaattgga aactttcaat acatcctatt aaactgaaaa ataaatgata
    11581 cgagaacgta atatttttac tagaaatacc gctctaaaaa tagcaatgtt taaatcaatc
    11641 ctactcttta atattctgaa tttattgaca agctcacaaa caggtgatac acttatctat
    11701 aatgttaggt aacctaacaa taaactgtta ttttaggagg ttcttatgtc actcatatct
    11761 tataagaatg ttaacaagta ttatggagat tatcacgccc ttcgtcagat taatttggaa
    11821 atagagccag ggcaagtggt tgttctatta ggaccttctg gatcaggtaa atcaacactc
    11881 attagaacaa tgaacgctct cgaatctatt gacgatggta gtttggtcgt gaatggacat
    11941 gaattagcaa atatttcatc taaagaattg gtcaacctcc gtaaagaagt tggtatggtt
    12001 tttcaacatt ttaaccttta tccccacaaa accgtgttag aaaatattac attagctcct
    12061 attaaagtat taaaacaatc taaaaaagaa gctatggaaa ttgctgaaaa atatttaaaa
    12121 tttgtcaata tgtgggaaag aaaagattcc tacccatcta tgctatcagg tggtcaaaaa
    12181 caaagaattg ctattgctag agggcttgcc atgcatccaa aactcctcct ctttgatgag
    12241 cctacctctg cccttgatcc cgaaaccatc ggagacgttc tttcagtgat gcaaaaattg
    12301 gcaaatgacg gaatgaatat ggtcgttgta actcatgaaa tgggattcgc acgagaagtt
    12361 gctgatcgta ttatttttat ggctgacggg gaaattttag tagatactac ggatgtgcaa
    12421 gacttttttg ataatcccac agaaccacgt gccaaacaat ttttaagaaa cattatcaac
    12481 cataccagta ctgaccttta ggaggacgaa atatgctgag acgaaaaaga ttaacgctct
    12541 atctattatc ctgtattttc attttcctgt tattttatcc caactcaacg tcagctaatc
    12601 aattatcaga aatcaaaaaa tcgggtgtcc ttaaagttgg agtcaagcaa gatgtgccta
    12661 actttggtta ttataatgct gagacgaatc aatacgaagg aatggaaatt gatattgcca
    12721 aaaaaattgc caaatcctta ggcgtcaaac cagtttttgt accgacaact gcacaaacca
    12781 gagagcctct gatggacaat gggcaaattg atattctaat cgcaacatac actatcacgc
    12841 ccgaaagaaa agctaactat aatatttcta aagcttatta tcacgatgaa attggatttt
    12901 tggtgcgtaa aaatagtcat atcaaaacta taaaagaatt ggatggtaaa cacattggtg
    12961 tggctcaagg tgccactaca aaagtcaatt tagaaaaata tgctaaagaa cacaagctaa
    13021 aatttagcta tgctcaatta gggagtttcc cagaattagc gatatcgctg tacgctaatc
    13081 gtattgatgc tttttcagtt gataaatcta tccttagcgg ctaccttagc cctcatacaa
    13141 ccatattaaa agaagggttc aatacgcaag aatacggtat tgcaaccagt aaacaggaca
    13201 aagtcttgat tccctacgtt aataagttac tggtatcatg ggaaaaagat ggtagtttaa
    13261 aacacattta tcaaaaattt aagcttaaac ccgctaaagt aaaaaaagaa tagggaggat
    13321 tttttatgac tttattaaac atcagtcctt ttgccatttc aaggtggggc gctttcttta
    13381 accattttga cctattcttt aaaggcttct tatatactct aggtatttcg tttggtgcac
    13441 tcctacttgc tctgatatta ggaattctct ctggtggttt atcaacttct aaaagtaaag
    13501 ttggtaagtt gatatcacgt atctatgttg aagtttttca aaacacacct cttttggttc
    13561 aaatggtttt tgtctactat ggactagcaa tcattagtaa tggtcatatt atgattagtg
    13621 cttttttcac cgctgttttg tgcgttggcc tataccatgg tgcctatatt tctgaagtta
    13681 ttcgctcagg aattgaagct gtccctaaag gacaaacaga ggctgcttta gctcaaggat
    13741 tcacagctaa tcaaaccatg caacttatca ttttgcctca agctatcaga acaattttac
    13801 caccaatgac caaccaagtt gttaacctaa tcaagaatac ctctaccgta gcaatcattt
    13861 ctggagccga tatcatgttt ttagctaagg cctgggctta tgatacgact aattatatcc
    13921 cagcttttgc aggagctgct atcttctatt ttgttatttg tttcccacta gcaagctggg
    13981 caagaaaaca agaagaatta aataagaaaa cttattctta aggaggtttg atatgtcatc
    14041 agtcttaaca ctaacaaata tactatttct ccttcaaggg ttcggtttaa ccttatatat
    14101 atcttttatt tccattttac tatcaatgtt ttttggaact ctattagcaa ttatgagaaa
    14161 tagcaaaaat cctatctgga aattaatagc tagtatctat atcgaatttg tcagaaatgt
    14221 tccaaatcta ctttggattt ttatcatttt cttagtattc cagatgaaat cagtcagcgc
    14281 tggtattact tcgtttacaa tttttacctc cgctgcttta gcagaaatta ttcggggagg
    14341 tttaaatgga gttgataaag gacagacaga agctggactt tctcaaggat tcacttattt
    14401 gcaagttttc attattatta tttttccaca agcttttcgc aaaatgttgc cagccattat
    14461 ttctcagttt gtaacggtta taaaagatac atcactactt tattctgtta tagctattca
    14521 agaaatcttc ggaaaaagtc aaattctaat gggaagatat ttcgaagctg gacaagtttt
    14581 tacactctac gctattataa ctgctgtcta tttcattaca aactttatca tctcaagttt
    14641 ttcacgtaaa ctttcaaaac gttgggagca agctactgag taattaaaac gaaggccttt
    14701 tagaccttcg tttttttata tcttatttaa attaaattat atatgccttt ttgactatct
    14761 ttttatatct tctcaaatgt ccgcaaagcc ttattttatc tgctctacgg gcattttgct
    14821 tttgtggtaa acctcacata tctaggtcta tctttttata ttttcgctat caaacgtggt
    14881 taaaatcgtg gtaaatactt ctatgcgatt agacgctgaa cctctgattt tgcggtatct
    14941 atggacgcat gagcatacca gttcattgtg atactgatgt ttgaatgtcc catgatatac
    15001 tgtaaatctt tcgggttcat gttcttgctt gccagccttg tgcagaacgt atggcgtagc
    15061 gtatgcggtg tgatatgtgg caagggattg tccttgtgtt gcttgttgta tttctttacc
    15121 atacggacaa ataaggcatt gtaatcaatc gcaactttgg gcttgccttt atgattgaca
    15181 aacagaaaat tgctccgtcc gtctatcaca aatggttctg cctttgggcg ttttttcata
    15241 acccgttgaa atgcctgtat tgtttctctg cttaatggca cttgccttgt tccgttcttt
    15301 gttttaggcg tttcaatata atagccctgt tccttgctct ttaataactg gtggtcgata
    15361 atcacaactt cattcttgaa atcaatatcg gctactgtca gtccgcacag ttccgagata
    15421 cgaagtccag tctttaacag tatcagcaca tcatcataat acttatgata cacgttgtcc
    15481 gtcttaataa atgacagtag ggcttgttct tgttcctctg tcaatgccac tttctctttg
    15541 gtatcatttt ctaggacttc acttaacttg aaatcaaaag ggttcttcct tacacaatcg
    15601 tcttgtatgg cgatatagaa tgacgctttt aacgagcgtt tatggttgtt aatagtattg
    15661 taggaaaagc ctttctcttt catgcgtaac gcccattcct tagcgtcaga gggttttatc
    15721 gtatcaatgc tcctagcacc caacttgtcc tctttcaata accgcatgag ctgttcccgt
    15781 tgtttcattg tgcttttctt cacgtttgcc ctctgttcgt tctgtttggc gtagagttgg
    15841 caaagcgtca ttttcttgcc tgtgctgtcg ataccgtcct caatatcccg tcttatctgc
    15901 tgttccagtt cacgaagtga ggttttttcc cgttttccct ttggtgtcgg gtctgtgggt
    15961 gtcaatctcc aagcatacac atattttgta tttccaaatg cgtctacata tttatacaag
    16021 tattttccgt ctgttcgttg gctctctcca gtatgcagga tacgtccttt gttatcccgt
    16081 cttttttctt tcatggtgtc tgctcctttc cttgttggaa agagccttga tatgacttgt
    16141 gttcatcata acacatacaa ggctcatttg cattagattg cgtccaattt atcaataacc
    16201 tgttcaaact gtcgtcgttt aatctgtatg cggttgccat tcataatgag ccagccagcg
    16261 tccttgtttt cctctgccaa gcgtcttaac ttgttttctc cgatacggaa atactttgac
    16321 gcttcttcaa tggtaagggt gtatttttcc catacgggaa tatcctgtgc attactcata
    16381 aaacaccccc tcactttcaa ttacgtctgc tatgtccgta cacatttgtt ccacaagcat
    16441 ttcctgtttt tcagaaaggt gggtacattc atcaatcatt ttcatgtagt ttgagcctgt
    16501 cagcctgtcg atttcctcta tcatcacgat tgacggcatg acctgtcttt ccagccattg
    16561 cagagtgcgt tccatattga caggtacggg gttcatttca aaaggtaaac tatcatggga
    16621 aatgaatatc tcccacagcg gatagtcggg aaattgcaca aaatcagtaa taaggtaaaa
    16681 caccagccca tgagggttat acgtcaataa cagttcctca actgcctgta ctgccttttt
    16741 gtcacgcaga cgaatttcaa aacggttgat aatatccgta tgtttctttt tagttgcctg
    16801 ttccttttgt ttttcataca ggcagaaata ttttgtgctt gcttttgagc cgatataaag
    16861 ggtgcttgct aaattgcggt tttttccgct tagtttaccg ccttgcacat tttcatagtt
    16921 tttacagcga caatccgcac cgccattttt gtatttttca gataaaacgg gaatatccag
    16981 cagaccgcac atatcgttga ttgccaagtc aaaacgtcgg ataacaccgc cacagtcaag
    17041 acagcggttc aagaatgaat accaatccct gttctgtgcc tgtaaaacat attccatatt
    17101 gcgtgagcct gcccctttta attccaaaaa tacgcccatg tgttcatcat cagaagccat
    17161 aaccttgatt tctccgtagg cgtaatgctc tttatagcca taatatccat agtcataatg
    17221 gataaaataa tctgctttca tgccaagcac ctttcggata atttccaatg cgtcagtcgt
    17281 tggaaaacgg acagacagat aatcaacgag cagataaaaa ggcttatcgc agatatagta
    17341 attcagacaa cgcaggataa tattttgtgt ttccttgctg gcgttggctt ttccgttctc
    17401 aaagcggttg atactatatc ttgatacatg ggtaagactt gcaagttctc cttgtgaaag
    17461 accgcttgca agacgtttat tcttcatttc aagtgcaaat tctaaattgt tcattgtatt
    17521 cctccaatcc cacaatagcc ttgaaatacc tgtaaaccac tattcaaaag aatgtgaaat
    17581 tactatcttc ttaaaactcc tcattactcc ttacagcttt ggggttgtat ggtttttgac
    17641 ttgtcttttg tgaaatagtg cccccctgtt agataacggg ggcacagaca aggctcgcag
    17701 gctcgccagc cgacaaagca tttcttcccc ttgacacaag gtgtcatggc ggtaagcctg
    17761 agaggcttcc gccaccgtgt cagaaatact ttgtcagcaa gttctgtttt gccttttcta
    17821 acaagttcca tttcaaccga tattcatatc tacggattta atcagatagg tatttctttt
    17881 tgaatgtttt tatatttcat tctattgtgc ttattcgatt acaagaacat agtaattctt
    17941 atatgcttat ttgtcaatat ctttttttgc ctttacaaat catattaagc atgatagact
    18001 tataatagcg aagatataac gagatatgga gggttatgca atgaaaataa gccatttagg
    18061 aaataatata cagaccataa gaaaatttag gggaatgaaa caacaggaac ttgcggacaa
    18121 aatcggtatc aatatgcaga gcctttccaa gattgaaaga gggttgaact atcctgccta
    18181 tgaaacgctg gaaaagataa tggaagtgct ggacgttacg cccaatgaat tgttgtcggg
    18241 agaatggaag tatgtcaatc aatctgaaaa ggaagtctgt cagtttttaa gagccgaaga
    18301 acgtctaaat gcagaactga aacatggaca ctatgataac ttctttgaca gcgaggaaga
    18361 atggctggaa tatgagttag aaaagttacg ggaatatatt acagactata tcaacgggaa
    18421 aagcattgaa gcgtccgacc tttacccaat caaagaattt atccagcatt tgaagtttca
    18481 aaaattatta gaccgctatg atgatttata cagtatggat atgtttgggg aaagcataga
    18541 gggacataag tataagacac cttatcaagt cgtgaaaatg ataaacccga actcaaaaga
    18601 agatatggaa ctgttacgag aagtattaag gaataacaat tttgatgatg aggacgagta
    18661 acttttttcc tcttgcgaga agatacagca aacaaaaagc ccgatcaaga gtgcaaagca
    18721 ccaccaatgg tggcaaagct cttgacaggg ctttttcttt tctgttgtgg gcaatcaaga
    18781 ggaagaaagg gataaaatct gatttgtggt aaatcaggca aaggcaacgc aggtatctca
    18841 atttgatttg gtacttgaaa agaacgggag taattggaag attatagaat aacaaatatt
    18901 ggtacattat tacagctatt ttgtaatcac gtactctctt tgataaaaaa ttggagattc
    18961 ctttacaaat atgctcttac gtgctattat ttaagtatct atttaaaagg agttaataaa
    19021 tatgcggcaa ggtattctta aataaactgt caatttgata gtgggaacaa ataattggat
    19081 gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt tatcatgtga
    19141 ttctaaagta tccggagaat atctgtatgc tttgtatgcc tatggttatg cataaaaatc
    19201 ccagtgataa gagtatttat cactgggatt tttatgccct tttgggcttt tgaatggagg
    19261 aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgcg ggaaaaacta
    19321 ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga agcgtggaca
    19381 aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt acaattcaga
    19441 caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac acgccaggac
    19501 atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg gcaattctac
    19561 tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat gcacttagga
    19621 aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga attgatttat
    19681 caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc aaacagaagg
    19741 tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa tgggatacgg
    19801 taatagaggg aaacgatgac cttttagaga aatatacgtc tgggaaatta ttggaagcat
    19861 tagaactcga acaagaggaa agcataagat ttcataattg ttccctgttc cctgtttatc
    19921 acggaagtgc aaaaaacaat atagggattg ataaccttat agaagtgatt acgaataaat
    19981 tttattcatc aacacatcga ggtcagtctg aactttgcgg aaaagttttc aaaattgagt
    20041 attcggaaaa aagacagcgt cttgcatata tacgtcttta tagtggcgta ctgcatttgc
    20101 gagattcggt tagaatatcg gaaaaggaaa aaataaaaat tacagaaatg tatacttcaa
    20161 taaatggtga attatgtaaa atcgataagg cttattccgg ggaaattgtt attttgcaaa
    20221 atgagttttt gaagttaaat agtgttcttg gagatacaaa actattgcca cagagaaaaa
    20281 agattgaaaa tccgcaccct ctactacaaa caactgttga accgagtaaa cctgaacaga
    20341 gagaaatgtt gcttgatgcc cttttggaaa tctcagatag tgatccgctt ctacgatatt
    20401 acgtggattc tacgacacat gaaattatac tttctttctt agggaaagta caaatggaag
    20461 tgattagtgc actgttgcaa gaaaagtatc atgtggagat agaaataaaa gagcctacag
    20521 tcatttatat ggaaagaccg ttaaaaaaag cagagtatac cattcacatc gaagttccac
    20581 cgaatccttt ctgggcttcc attggtctat ctgtagcaca gcttccatta gggagcggag
    20641 tacagtatga gagctcggtt tctcttggat acttaaatca atcgtttcaa aatgcagtta
    20701 tggaggggat acgctatggc tgtgaacaag gattgtatgg ttggaatgtg acggactgta
    20761 aaatctgttt taagtatggc ttatactata gccctgttag taccccagca gattttcgga
    20821 tgcttgctcc tattgtattg gaacaagtct taaaaaaagc tggaacagaa ttgttagagc
    20881 catatcttag ttttaaaatt tatgcgccac aggaatatct ttcacgagca tacaacgatg
    20941 ctcctaaata ttgtgcgaac atcgtagaca ctcaattgaa aaataatgag gtcattctta
    21001 gtggagaaat ccctgctcgg tgtattcaag aatatcgtag tgatttaact ttctttacaa
    21061 atggacgtag tgtttgttta acagagttaa aagggtacca tgttactacc ggtgaacctg
    21121 tttgccagcc ccgtcgtcca aatagtcgga tagataaagt acgatatatg ttcaataaaa
    21181 taacttagtg tattttatgt tgttatataa atatggtttc ttgttaaata agatgaaata
    21241 ttttttaata aagatttgaa ttaaagtgta aaggaggaga tagttattat aaactacaag
    21301 tggatattgt gtcctgtatg tggaaataaa acacgattaa agataaggga agatactgaa
    21361 ttaaaaaaat tccccctcta ttgtccgaaa tgcagacaag aaaatttaat tgaaataaag
    21421 cagttcaaag taactgtgat tacagagcca gacgcaaaga cgcagagccg ataaaatgag
    21481 attaatacaa tctcatttta tcggctcttt ccgttatgta tggattcttt taattagtct
    21541 tcgatgtttc ttgcttcgtt gataccgctg gctaaagatt ccattaagga tagttctttg
    21601 tctgtaaagc tatccatgta tttctctatc tgtaatcgtc gggtgctttt taccaagtta
    21661 ttagcaggta agaaaaattc atcaacggaa acatgaagta acgatacaag gtcataaaga
    21721 acttgtatgc tggggtgttg ccctttattt tcaatattag ttaagtaccg tgggtcaatt
    21781 tcaatcaatg ctcccacttg ttcacgagtt aaacctcgtt tcaatcgagc ttctttaatg
    21841 gctaaaccaa aggctctaaa atcatattta tcttcttttt tacgcatagt agaccacctc
    21901 tatacatttt attgttccta ctgaattaaa aacaggtata gaaaaacgtg ttatatggtt
    21961 tataggttta tatttaataa aaagcactac taaacgccaa taaaaaaaac cgttatatgg
    22021 tagtgctatt tacgctgtta aaatattgta tattacttcc aaatggcggt ttgttggagg
    22081 tcaacgtcgc catgaagtac atcatataca ataaatttcc ttacattggg ttcttgtcaa
    22141 aaaaagtcgt ctatctgcaa tagataagta cgtccaccaa tgtggtttta taaatcatat
    22201 agatagaata acagaagcat gtaaacagag aaataaatct gtttatatgc ttttttggct
    22261 attcagaact tttttacaaa gtttatttat cagtaatgca acaaatcccc ctttcacatt
    22321 gggactaaga gtgaaaggag ataaacgagc aaggctcact tcctttccta gacagaaagg
    22381 gggtgagaaa catgaaacca tcttcttttc agaccacaat agaaaatcag tttgactata
    22441 tctgtaaacg tgctatggaa gacgagcgaa agaattatat gctttatctt tcaaggattg
    22501 caaagcgtga ggtgtccttt tcggatgttg gcgattatct tgttagccag tttgcgacaa
    22561 cagataacta ttcaactgac tttcagattt ttacactcaa tgggttatca gtaggcgttg
    22621 aaaatgattt gttgagtgaa gcattacgtg agttgccaga caagaaacgt gaaattctac
    22681 tgctgtttta cttatgggaa tttcgctatt tttagaattg cctgcaatag tttcctactg
    22741 ggtagttaca gtgatgtgct ttgtggctat gggtgtatct actatgtttg ttgttcaaat
    22801 ttacactctt gttcagacac aaacaccacc gcaattagta ggaaagataa tggctacgct
    22861 ggtagcagtt gctatgtgcg gtcaacctat tggacaaatg gtttacggta ttttattcga
    22921 tgtatttata caagaaacat gggttgttat gtttgtagct tctattgctt caatcatgat
    22981 ttcaatatat tcaaaaagaa tttttcaaaa gttagagata taatacaagg ggttaaatag
    23041 tatgttaggc taaaaacaat atataaaaga aaatttgaaa tctgctaaaa ggatagtgtt
    23101 ataaaagaaa gagtaatatt gcagtattta aggctcgcac aaatatatgt tttgtgtggg
    23161 cttttttctt accatttttc tgtatttcta aagggtataa acggtattcc gtttatatat
    23221 ttcagcatta gcagaaaggg ggtgagatta tgaggacatc ttctttcgag catatcgtca
    23281 gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa agtcgaaaca
    23341 gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc gaaacgaaac
    23401 aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt aaagtcttgc
    23461 actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga ctgtcagaaa
    23521 aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga aaaatttcag
    23581 aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga ctaaggaagt
    23641 taaaaatgct tttgagcgaa gggaagtaaa gaaatgagta aagattatgg ctatccctct
    23701 tttgaattga tttgcaaagc gtcaagcgga gatgaaatgg cagttaaaga gattttgaag
    23761 ttttatgacg gttatatttc taaattatgc ttgcgtcctt tttaccactc tgaaagtggt
    23821 aaaatcatta tgcaggttga tgaagaacta aaaggagaaa ttcatacaga catgatgaaa
    23881 gcaatattga aatttgaaat cggagtgaag taatcaactg tataaaaaaa tcgggagata
    23941 cacagtttcg tgctatctcc cttagtagtt ttacggtcat atcaaggctc attcaatcgt
    24001 ggtaaattcg tggtaagatg agtgtttttc tatacttcaa aatgcttgaa tgtatagtgt
    24061 ttatgcggat aattgaaaat tagatataaa atttctatta atcattcata tgatctctag
    24121 aacgttcaat gatttttcca gtttctgcat caatttgata atcaacttca tttttatttt
    24181 tcaggtccac aaagtcaata tcataaactg attttccatt ttcaatttct tctttgatct
    24241 taagattact gacttctgat tcctctatat tagcatcttt aaatgcaatc tttttggctt
    24301 cttcttgact tattttatga gatttttgac ttgacgatga acttgactga gaagtcgatt
    24361 ttgaaaagcc attattatcc atgtcccgct caaggatttg tccagtctta gcatctacat
    24421 gatagctgta ttctgcgctt ttggtattga attcaatttc gtagctccct ctaaagtttt
    24481 caaattcttt gtcaattttt tgatgtatga catccttctt agccacttta gcatctttat
    24541 aagcaatagc taacgcttga ttagttgtct tttggaattg tgattggtaa aataatacac
    24601 cacctccaag gataacaaca aggactaaaa gaactcctaa aaaaacttta tttttcttaa
    24661 tcatacagca ccttctttct attattcaat tgtattttaa cataagctag acactttttc
    24721 aattttgacc actcgtttat aaaaatatgt ggtcaataaa aaccatttga cattaatacc
    24781 gcaaattaaa agataccata gtatcatggt atcttttaat agtatatgta tcagttgatg
    24841 cgcattttct gcacagcctt cttcaagtat ttgacttgtt tatcaaccga ttgcttagca
    24901 ctacgccttc caaatgcgta atatcctttt ttattgaaat aactacaatc gtaatcctct
    24961 tctttaaaga aaaaccattt ccttctagat agatttggcg atctataacc tctagtcata
    25021 tcgtttcgaa attttgggtc taactttaca ggatcaacat ctaattcttt atgacgtgta
    25081 ttatttttaa aagcataatt aaagctagca ccgttgataa tgccattacg tgtgattact
    25141 tctgcgtcaa tctcgtttaa aaagtttcct ttgtcatcaa taataaattc cgtatggaat
    25201 gaaattaaaa gcttactatt aataaccggc tcatcatctg ggaaaccaaa gatttcacca
    25261 tttctccgac gtttattatg caagcgagca gattcatagt aggcgatact atctggttct
    25321 cttaaacatg ataggtagtt tactaaagca tctctatcag tcataccaac atctttaaag
    25381 tatcgtcgaa catactgtgc ttgctgacta gaaatcaaat agcggaattg atggagtttc
    25441 ctatttaata cagtatcttc ggctagacta ttcccaggaa aagaatcata aataacttgt
    25501 gataaggcta tccagaaatc atcatgcgga gctaactcat ctgaaaactg tgcgaccaag
    25561 tattgtgttt ctgataattc tcctgacaat tcagttggca ttttagcctg ctctaacacg
    25621 aattctaaag ctttgagggg tgcttgtaaa cttaaagaag tccaaaaata cttaaaaata
    25681 ggagagccaa cttttatatc atcaatagcc ttgagtccct ttaaaaagag taatagatca
    25741 tctttaggta tgctccattt atctgctaaa gacagtatca tttttatatt ttgataataa
    25801 ttttctatcc cttcaactgt aaaaggaata ttaagagcgg tctttgccat atctctcccc
    25861 tccaatttta cttattatag caaaaaggaa agcttaaaac caagaaatat cttcactaaa
    25921 aaatggctct cactagccat tatgttttta aacaagatac tgcggttttt cgattttata
    25981 ccctactcct ctaagtgttt ttatatattt aggatattta ctatcatctt cgattttgtc
    26041 acggatatta gaaatatgca ccataatcgt tgtctcatca ccttctaaat acctaccatt
    26101 aatcttctca tagagttgtg ttttagtata aatctttcct ggtgatgaca ttaagatatg
    26161 aagaatctta aattctgtag cagtcaactt gatatgttta ccatttttag ttagttcaac
    26221 atgttcctca tctagttcca attcccctat ctttataaat tgatttttag gtttggcaag
    26281 actattgaat tcatagtaac gacgcagttg cgaattaatt cgagctaaaa cctccaaaga
    26341 attaaacggt ttcgtgataa aatcatctgc tccaatattc aatcctaata ttctgtccat
    26401 atctgttgtc ttagctgcta gcataataat aggaatctga ctatcttgac gaatgagtcg
    26461 cgtcaactca tatccatcta ttttaggtag cgtaatatct atgattgcta aatcaatctg
    26521 atgttgctga aataaagaaa aagcctcctc accatcacta gccatcacaa ccttataaag
    26581 agctccctct aaatacgacc ttaataactc tattgtttcg tcatcacctt gcactactaa
    26641 tactgttcgc aatgctccat cccccttttt attaatacta ttttccttta tgtgtcaatc
    26701 atattaatta taccaaaaaa tctaattttg tgaatgttat atgagattat ttatctataa
    26761 ttagcttata attaaacatc aagaaaaaat tagcatctaa aaaactgatt ctgcatcata
    26821 cagtaatcag ttcacatcat ttattaatga tttttataat gactaataaa atttatttaa
    26881 cttcgtcagg tctagatttt cttgcaatat cagctaaaat actttctaca aattcctcta
    26941 tagattttgt ttcagtcgct ttacttccat agcgacgcac atttacggct ttttcttcca
    27001 tttccttatc accaacaatc aattgatatg gaattttttg ggtttgcgcc gcacgaatct
    27061 tatattgcat cttttcgtta cggtcatcca cttcagcacg aataccacga tctttaaggg
    27121 cacgagccac ttcccaagca taatcaatgt gggcttcatt tgaaattgga ataacagaaa
    27181 cttgttgtgg agcaagccaa gttggaaagg cacctttgta agtttcaatt aaaatagctg
    27241 taaatcgctc cattgttgaa ataccaccgc ggtgaatcat aattggacga tgttcttcac
    27301 catctgcacc gatgtattta agatcaaagc gttctggaag aaggaaatct agttggatag
    27361 tagataatgt ttcttcatta ccaagagcag ttttcacctg gatatccaat ttaggaccgt
    27421 aaaaggctgc ttcgccttct gcttcaaaat aatctaatcc aaaatcatcc atagcctctt
    27481 ttagcatagc ttgtgcattt tcccacatct catcgttatc ataatactta tgtttatctt
    27541 ctgggtcacg ataagataag cggaaacggt aatctgttaa gttaaaatct tcataaattt
    27601 ctgctatgag attaagagct ttaagaaatt catctttaat ttgctcaggt gtaacgaaaa
    27661 tatgggcatc gttgagtgtc atttcgcgta cacgttgcaa tcctgtgagg gcccctgatt
    27721 tttcataacg gtgcatcata ccaagttcag cgatacgaat tggcaattca cgatatgagt
    27781 gaacatgatg tttataaact tcaatatggt gtgggcagtt cattggacga agaacgaatt
    27841 cctccccatc tcccatatcc attgttggga acatatcttc acgatagtgg tcccagtgcc
    27901 cagaagtctt gtaaaattca acagatgcca tcggaggtgt gtaaacgtgt tgataacctg
    27961 aagcaatctc cttatcaaca atgtaacgtt ccaattcgcg gcgaatagtt gctccatttg
    28021 gcaaccagaa tggcaatcct tgacctactt caggattaac cataaataaa tcaagttctt
    28081 tacctaattt gcggtggtca cgttccttag cttcttcacg gcgtttaaga tatgctttca
    28141 aatctttttt atcaaaccaa gcagtaccat agacacgctg catcatagca ttatcactgt
    28201 taccacgcca gtaagcccca gcgacattta gaagatgaaa gacttgaata cgtcctgttg
    28261 atgggacatg aggtccacga caaaggtcta caaattcacc ttgacgataa acagttaaac
    28321 catcttcagc atgttcacta attaattcaa ctttataagg gtcatcttta aaaagttcta
    28381 acgcttcttc tttactgatt tcttcacgaa tacaagggtg attttcctta acaatttttt
    28441 tcatttcttc ttcaatacga ggaagatcat catttgaaat ttgacctgat ttattatcag
    28501 tatcgtagta aaaaccatcg tgaatagctg gacctacacc taaacataag tctggaaaaa
    28561 ggcgttttgc agcttgagca aataaatgcg ctgctgagtg acgaagaact cctaaagcat
    28621 cttcgtggtc aggtgttaca atctcaatac taccatcttc ttcgatagca cgtgttgtat
    28681 caataagttg accattaaat ttaccagcta aagctttttt agctaaagag ttagaaattg
    28741 attgtgcaat ttcaaatgtt gtgatgccag attcaaattc acgaattgca ccatctggga
    28801 aagtgatttt aatcatcttg ttctcctttt cttaataact gttagggtga taccaattcc
    28861 taaacacatt aattcactaa taaacaaagg gattgacctt tgattgaatt ccacattatc
    28921 ctagataggg ataataagta taacattaga aaagcaatct gcctctttta tgcaaccact
    28981 tcttttataa aaaaagagcc tttacatcca aaaggacgta aagactacgt ggttccacct
    29041 caatttatcc aacacaaaaa ctcgagtata cttgtgtcag acctctagtg gcgataaggg
    29101 aaccaccctt ttatgtttac ataaaatcaa ggaagtagta ctaagttctc taattgcact
    29161 ctttccacca accagtgctc gctcttgaaa tctattagaa cctaacatgt cttcattttt
    29221 tattataaca atatttttta taatttcaag acatttacta attttattcg tctcaatcta
    29281 aaaaatctcg aatagatgaa atacgacgtt taggtgcttt taccactttt ttagaggctc
    29341 ttcctgtaaa ggtaaaaact ttcttaggta aatataccgc ttttctgaca aaacgaagag
    29401 tagaatggct atccttattt cctatcgtca gagagacttt ttctttcaca ctagcttttt
    29461 gtgaaatcag aaagtctagg taaaaagcat atacagactt gctgaaattc tcagcagaaa
    29521 tctcatataa tttctgttca taagcttctt gagtcatttt gggtgtctct gctattgcat
    29581 ctaggatagc atctgccaaa tcactttcct tcttgtacag agttccaaac attttatctg
    29641 aaataacatc atctagataa ggattgcttt gcgcaatgat tggacgtcca ctcgccagac
    29701 tctcaatgta agttaatcct tgcgtttcac ttgttgaagc actaataaag aaatcacatg
    29761 ctttatagta gatagccact tggctatgct caaccatacc tgtaaaaata actgaatttt
    29821 caagttctaa agagtgaaca agttctttta gatctgaaag ataaggacca tctccaacta
    29881 tcactaattt tacgtgagga ttttcatcaa cgacagctga taaatgcatc aagattgctt
    29941 gaatattttt ttcaaaagat acccgtgata aactcaatag cattgtatca ttatccgcaa
    30001 ggccaagctc cgttcgtaga ttcttgattg tttcttcaga gatatcctct cgacgatagt
    30061 tctcaacagg aatacctgtc ggaataactt gttttggaat tttgacaccg taaccatcta
    30121 gaagattgag aacaattcga gaaggacata tgacaccatc caaatcactt aggtaggtac
    30181 gcatgatata tttgaccata cttggcttaa tcaatttgcc ttttgcgata taaccaacgt
    30241 aatcctcata ttgagtatga taagtatgca caacaggtat tcttaaagct tttgcaacta
    30301 attttcctaa aagtcctaaa ctgaattctg tttgagtatg aataatatca agttcgtagt
    30361 ctttagctat cctataagca gaaattaaac ctctataaac tactcgacga tctgtaaaag
    30421 aaataaaagg gacacttggt aaacgaataa ttgtagggtc ttcaaaacgt ttgacattac
    30481 ggtcggtagt tgtgaaaata taaacttcgt gtccttcctt ctccaaccct tcctttaaag
    30541 ttcggatact tgttgaaact cccgaaactt ggggaaagta tgtatctgta aatagaccta
    30601 tacgcatctt acaactccat tactttttga taaacacttg ccaattcatg ggcaattcgt
    30661 tcgatacttc gactctcagc cacacgataa ccttctttaa tcttatcact cttaccagat
    30721 aagactttat ccaacttttc aacaaaacca tcaacatcag tcgctaattc aacactgtct
    30781 tcggtcaccc aaccgtgata cacagggata tctcttaaaa ccacatgctg atgactagct
    30841 aaagcctcta aaactactat cccttctgtc tcttcacgac tcggaaagaa gaaagcatcg
    30901 ctagcactca tggcaccttc ataaacatca ccttttatgt aaccagcaaa agtaacattt
    30961 gagggatgtt gcttggtaac gatttgccta acctttcgag gaataaccca tttatttgtt
    31021 tctccaaacc aaataaaacg aatatcaggc atttttgcag ctacttcaac aaattggtca
    31081 attcccttac gcatgaaata aagaccggcc cccataacaa ctttatcatc cttacttaag
    31141 tgaaaatagt gacgaaatgc agcttctttc ttttctgacc tctgatagcg acttagatca
    31201 atcccgttag ataaaacaaa aattggtttc ttgatgccat atgcttttat tagttgttta
    31261 gaataatcag ttggggttat aatggcatct gctttttgat aaaaacggca aaggtaccat
    31321 ttaaaaagcg gggatactaa atttgagcca ataaaagaat ttctaaagtc ctcttcagtg
    31381 gaatggccat gcataattac ttttttccca gtttttttag ctttactcat caaaagccaa
    31441 cttctaatac cataggtatt catatgaaca aggtcaaagt catctgtagg attagtggta
    31501 taatctatcc cagcaatttg aagagctttc tcttgatgtt tgatagcacg accgatacct
    31561 gactttttca agtattcttc agcctctaaa tacagtaaaa ctttcatagg tcttattata
    31621 tctcatttta cctttttttt cacctgattt atcagttagc ttcctctatt ttagggaccc
    31681 aagccgagat agacgcagat gcaactaaaa aatcacccca accatcatct cctataacaa
    31741 cctcttgttc acaattgcct aaataatcaa caaaagtttt gcctgcataa atttccccta
    31801 cttccatatg tttccatccc tgatcaccat ttgttaggac cacagctaaa caagaattat
    31861 gttcctcatc cccaagacaa gtccaaccaa tacaatttga atgcgtaaag taatcgactt
    31921 gttcgccaaa tacatagttt tggcgtagct cagccatttt atcaataacc tctttaaagg
    31981 atggttggcc aaattctcct tgaatgccat agtaatcacc ataaaaaaga catggagtcc
    32041 cttgttggcg taaaagtatc aagccataag ctaaaggttt aaaccaatct tccactctac
    32101 tttctagagc ttgtccactt tgcgtatcgt gattttctac gaatgtaaca gcatactcag
    32161 gattatcaat aacaagacta tcatcaaaaa ttgttcgcat atcaaaatca cggttttggt
    32221 gactggcatc aaaaaaattc atatgcaagg tgacatcaac aagagaaaat tgaaattgtg
    32281 ttgcctctaa atagtccttc attgatgttt ggtcagattt ccaatattcg ccaaagacct
    32341 ctaaatcggg ttttattttt gtacgaatat cattaatgaa agtttgaata aagtaagaat
    32401 cgatatgttt cacagcatcc aaacgaaaac cttcaatgcc tgtagtttca ataaaccact
    32461 tagcccaatc ttgtaagttt tttataactt caggatgctt aaaatcaatg tcattgtaca
    32521 tgagataatc gaaattacca ttttcatcat caatcaaatc atcgtctgcc caacctttat
    32581 tatcgcctac aatcataaag atgtctgttt cattattttt agcatcgtaa tctaaaccag
    32641 taaagtggta ccagtgccat ttaaaatcat tgtactcacc ctgtctacct gggaaatcaa
    32701 atcccgtcca tccttcaatc tcatagggtt cacttaatgc ttcttgacga ttttcaggat
    32761 tgactttgat gacttgaaat ttttctttat gatcaccatt ggctttatgg ttaagaacga
    32821 tatctgcaaa cggtttaatg ccatttgcct ttaacgactt aataagcttt agatactctt
    32881 ctttcctacc atattttgtt ctaattgttc cattctggtc aaattctcct aaatcaaaga
    32941 gatcataaac accatatcct acatcatcac tgctagttcc cttaaatgct ggtggtaacc
    33001 agattttact aattccaagt tttttaaggt ctgatataga ctcctctaat ttcttccagt
    33061 gattcccatc actaggtaaa taccactcaa aagcttgcat tatcaattca tttgtcatat
    33121 tacacctcta aaaaaagatt aggaagctaa gctaccctaa tcctctcaat atatcatgtt
    33181 aagtatcaga aagttaagcg ctaagctact accattctac cactattttt caaataacat
    33241 actctaacac taattatttg ttgtgccacg tttaacaatc ccgtgattaa gcactatttc
    33301 tttttcttcc aactcttctt tgtgcataat ttttgttagc atacgcatgc taacagcacc
    33361 caaatcatag accggttgac tgatagaagt taagttagga cgtgtatatt gtgcgattgg
    33421 cgagtcattg cttgtgataa tttcaaaatc ttcaggaaca cgtttacctg cctcaaaaag
    33481 accatttaac aaacctgccg ctaactcatc ctcagcaaca tatgctgctg ttgctccagc
    33541 attaatcaca cgttgagcta atgcaaagcc ttcagcataa cggtagttgg cttcaaaaac
    33601 caacccctct ttaaagttta agccattttt cttgagacct tctttgtatc ctgccaaacg
    33661 aaccttgcca ttaatatcgt caataagtgg tcctgataca aaagcaatgt ctttatgatt
    33721 gccagctaaa atatcaataa catcaactgc tgctgctttg taatcgatat taacacttgg
    33781 taattgatgc tcaaggtcca cagtacctgc taaaacaatc ggtgtgcgtg aacgagagaa
    33841 ctctgcacga atcttttcag ttaagtggtg tcccataaaa ataataccgt ctacttgctt
    33901 agcaaacaaa gtattaacaa cattaacctc tttatcatcg tcttcatcac ttgaagcaag
    33961 gacaatgtta tatttgtaca ttgctgcaat atcatcaata ccacgcgcta aaattgaaaa
    34021 gtagctattt gcgatattag gaatgacaac accaacagtt gtcgtttttt tacttgctaa
    34081 accacgcgcc acagcatttg gacgatagtc aagacgatca ataacttcaa ggactttctt
    34141 acgtgtattt tccttgacat ttttatttcc atttaccaca cgactaacag ttgccatcga
    34201 aacacctgct tcacgggcaa catcataaat cgtaatcgta tcatctgtat tcatattgat
    34261 ttaacttcct ttctatttga aaatgacgct ttcactgata ttttatcacc ttttggaaac
    34321 actttcaagc ttttataaag cttttttatg aaaaactttc aaaatactaa ctaaacttga
    34381 ttttttccta tttttttgga aaaataatag gcataagaaa ctagcctaac tagactaagt
    34441 tccttttagg aagaagggtt attatgtcaa aacttaatcg tatacgccat cacttacatt
    34501 cagtccaagc tgaacttgct gtcttttctg atcctgtgac agtcaactat ttaacaggtt
    34561 tcttttgcga tccccatgaa agacaaatgt ttttatttgt ctatgaagat cgcgatccta
    34621 ttttatttgt accagcacta gaagtatcgc gtgcaaaaca aagtgtacca tttcccgtct
    34681 ttggttacat agattcagaa aatccttggc aaaaaatagc tagcaactta ccatcctttt
    34741 ctgtttctaa agttctagcc gaatttgata atcttaatgt tactaaattc caaggtctcc
    34801 aaaccgtttt tgatggtcat tttgaaaacc taacacctta tattcaaaac atgcgcctaa
    34861 ttaaatcacg agatgaaatt gagaaaatgt tagtcgctgg agaatttgct gataaagctg
    34921 ttcaagttgg atttgacaat atctcgctaa acaacactga aaccgatatt atcgctcaaa
    34981 tcgaatttga aatgaaaaaa caaggtatca ataaaatgag tttcgacact atggttttga
    35041 ctggcaataa tgcagcaaat ccacatggta tcccaggaac taataaaatc gaaaataatg
    35101 ctttgttatt atttgattta ggtgtagaaa cactaggtta tacgtcagat atgacccgta
    35161 cagtagcagt ggggaaacct gatcaattca aaaaagatat ttaccattta tgtcttgaag
    35221 cacatcaagc ggctattgat tttattaagc caggagtcct tgcttctgaa gttgacgcag
    35281 cagcaagaaa tgttattgaa aaggctggtt atggacaata ctttaaccac cgccttggtc
    35341 atggattagg tatggatgtc catgaatttc catctattat ggctggtaac gatatggaga
    35401 ttcaagaagg catgtgcttc tctgttgaac caggtattta tataccagat aaagttggtg
    35461 tgcgaattga agactgcggt tatgtgacta aaaccggctt tgaagtattt accaaaacac
    35521 ccaaggaact actctatttt gaaggataaa aaaagagctt taagctcttt ttttatgtta
    35581 cataaaagta catcatcttg tttctattag cattggcgcc ataaatgtgt atcaattaaa
    35641 ccacagtaag catctactgg tgaaactcct gtaaattctg attctccagc caatttcgca
    35701 actactgcac ttatcgtgct aggaagccca tcataagcat taatataagt ccctacctgt
    35761 ggcatatctg ctaaagcaaa tgcatgttgt actgaaatca caatagatgg tatttcatga
    35821 acgtaaaaag gctgatcagg ggtgccttta gctgctggcc aaataatacg ttgtgttgtt
    35881 cctccagcat tgacatctac caagttaata atcaagtcat agtgttctgt tagatttgcg
    35941 attggctgtt tttgtgcgta aacattagcg atagctgctg cccgctcctc ttgtggcaat
    36001 ttcataatac gctcttcggt agactcccag acagttactt catggccacg tgcctcaagt
    36061 aactctttca tgtaatccga tgcacgttgc ttgtttccag caatcatcgc accaaaacct
    36121 cccttatatc cttcaacgtt aacaagtaag atatgacgat aacgttctgg atttactgga
    36181 aagatatctt tttgtttatc ttttaccaga gttactgctt tatcagcaac ttcatcagct
    36241 attttttgcg actctaatgt gttgattaaa gccatagcct tatctttagg cataaagagt
    36301 tcttggcgac gtccctcata gttatgcaag cctaatttcg cttttagacc gagagtacga
    36361 cgtaaagcgt catggagacg ttcctctgtt agaatacctt tttcgtaacc ttctttcatc
    36421 cattgaatgt cttcatcagg gtcattaaag aataagaata aatcacaacc tgcttcaatc
    36481 gccgttggca acaaatctct ccttggcatt gaagcagtca ttccaaccat atgagaagcg
    36541 tccgttacaa tagctccatt ataacctaac tctcctctca ataactcatc taaaagtgtt
    36601 ttattcaatg aagctggtaa catatcatca aggtctcgct ctggatgcat ttctttttca
    36661 acgtttggta aatgtatgtg acctgccatg actcctggta aacctgcgtc agccaattca
    36721 ccataaatgc ggccaaatgt cgacatccac tcctctttac tcataggatt ggaagcaaat
    36781 gacaaatgat gatcacgctc atcaatgcca tcccctggaa aatgtttagc gaaaggtaca
    36841 atattatatt gcatgattcc tttcatatac tcttttgaaa gtgaaataat ttgatcaacg
    36901 ttagcacccc aatttcgact agctatgata ggattacgcc aattacgtgt caaatctaca
    36961 attggagaaa atgaagcatt acaacccaca gcagatgcct ccatccctgc gattcttccc
    37021 atttcataag catatttcgg atcatttgta gcagccactt ttatttcatc gccaacttta
    37081 gttccatccg tgacagcacc gtctccacca gcttctgtat ttgcagcaat caacatcggc
    37141 aatttcgact ttgtctgtaa aattaaattc tgatcataga tttcacttga actacctcta
    37201 ttatagcgaa cagcagcaat atgataccga tccaaaacat ctgttaaata ttcttctgaa
    37261 cgactagctc ccatattgaa aaaaagttgc ccaattttct catccaatgt catctcatta
    37321 attgtttttt caatccactc aattgcttct tgatttagat tgaagggttt ctttgttaag
    37381 tcaactaaat gtgtcatagc ctatcctttc tattagttat ctaaattagc ccaaaaggta
    37441 ttaatagatt ctttttgcct ttgctgttcg aattgacctt ttgcaaaagc ttcaccaata
    37501 tcttctctaa gaatttccca tgatttgact tccttaccaa caagtactgg gtaaaatgca
    37561 ataccatttt gttctgctgc agccagatca cccagggaat ctcctaccat caaaatagtt
    37621 tcacggcagt aaccacttgc aattaaagtg gcgataacat cttctttttt acctctatct
    37681 tgacaataga tatcatcaac atactttagc aatccctgtt cttcccactc ttgccaaaca
    37741 gcttccttgt tagcagaact cacgacataa acattaccaa attgacttaa tcgttctaaa
    37801 ctttctaaca cacctgaaaa cgctaaagaa tctccctcat aatttttgat ttggcgattc
    37861 acttcattgg accattctaa agccttctct aaatctctac taaaatcgtt ggcaagcgct
    37921 aactctaagg aattattgga caatgagtct gttgtttcga cccattcttt taatttatca
    37981 atatgttcta ctccggcaaa ctcaaggccc ataaccaatc ccacaaaccg attaactcca
    38041 cgagttcttg aataaagatt gactctatcc cactctttta aaaaacttgt tctttcttgt
    38101 tctgatatcc caaatatatc tgcagcaatt ggaccaaaaa agagttgatg cttatatgtc
    38161 attgtatcca ttgcgcaacc atcagaatct atgcaaaaag cgattttgtg atttgtatct
    38221 accatattat ctccagtttc taaatttatc taacttatga ggattttcca attagcaacc
    38281 tacccatcat ttagtggtaa gatcattaaa caaatttcct taaaaaacca ctcaactcaa
    38341 taatctctta tgtgttaagt ggctattatc ttgtaaatta aacaccagag taagcagcaa
    38401 atccaccatc aatcggaagg acaacgccat taacaaaact tgatgatttt tcatctgcta
    38461 aaaagaacaa tccgccaatc agctcacttg cttctccaaa tcttcccatt ggtgtattat
    38521 taagaatttt ctcagcacgc gcagttggct gaccgtcctc agtaaataac aagcttcggt
    38581 tctgatttgt taccaagaaa ccaggtgcaa tagcattaca acggatacca acttttgaga
    38641 aatgaacagc tagccattgt gtgaagttac taatagctgc tttagctcca gaataagctg
    38701 gtattttggt cagaggggtg aaagcattca ttgaagagat attaataata ttcgctcctt
    38761 cacgtccaac catatcttgg gcaaatactt gcgttggcaa taatgttcct aaataattta
    38821 agttaaaaac aaagctaata ccagcttcat ctaactcaaa gaaggttttt gtttctgaag
    38881 gtaagtctaa ttcgtgaaat tcattatcag ttgtagcttt aggattgttg cctcctgcac
    38941 cattaactaa aatatcagtt ggtccaagat cctctaaaac ggcttgatgc acttcttcaa
    39001 ggttttcttt agacaaaacg ttagctttat aagccttagc gataccacct tcttctacaa
    39061 tttcatcagc aaatgtttga gcagcttctt gattcaaatc taataatgcc actttagcgc
    39121 ctgctttcgc aaattcttta gccatataac cacagagaac accgcctgca cctgttatga
    39181 cgacaacttt attggtaaat gtaattgttt ttgacatctt attcctctca ttaacttcta
    39241 taagtattct actcttagct aagtacaatc atattatgat aataactttc caaatttctc
    39301 tttcaggaga ttggaacagg agaaatttgg aagcatcgcc ttattttgaa cctttaatca
    39361 cagcttcgta taaaccagaa acgtaagtgg ctcctaaagc tctatcgtat aggccatatc
    39421 ctggacgacc tgtttctccc caaatcatac gcccgtggtc aggtcgaata gcaccctcaa
    39481 acccaaattc atggcataac ttaataactt catacatgtc tattgacccg tattctgatg
    39541 gatgagccga ttctttaaat gactttccat cacctaattt aatattgcga gcatgaacaa
    39601 agtttacacg atctaactca aaagcacgtc gcgaaatctc taaaacatca ttctggggat
    39661 cagaagcgta agaacccaca cataaagtga tgccattact tttactatcg tacaatttca
    39721 caaaacgctc aatagcctct aacccagtaa taatgcgagg taaaccaaaa atagaatatg
    39781 gtgggtcatc cggatgaatg gccattttaa cgcctacggc ttccgcttca ggaataatac
    39841 gtttaataaa ataagtgaga ttttcccaaa gtttttcctc atcaatttca gcataggcat
    39901 ccatgatagc cttcatttct tctttactat agctggcatc ccatcctggc aaagataatt
    39961 caccgtttac tggatccatt tttttagaaa ccgtctcatc aaatatcaaa gctgtggaac
    40021 catctggata ttgatatgct aaatcagtgc gtgtccagtc aaatactggc ataaaattat
    40081 aacaaatagt atcaatacct tccgcagcca aatttttgac ggtttgaata taattatcaa
    40141 tcaagagatc acgtgttgga cgtcccaatt tgatatcttc atgaacaggt actgattcga
    40201 taaccgaaat tttaagccct gctgcctcta ccttttcttt cagctgttga atgcgctcac
    40261 gtgaccaaac ttctcctact ggtacatcgt atatcgctgt aacaatccct ttcattgttg
    40321 gaatttgacc aatattttct aaagtaacgg ggtcgtcttc accataccac ctaaatgaca
    40381 tttccatgat ttcactcttc ctttttagtt cttaaaataa ttaactgcat tttgataagc
    40441 gatgtcttta gccatacttc ctaaagcttg ataatcttct ggaacttctc cttcttcaat
    40501 ccattctcct aagtaggtac ataaaatacg tcgaaaatag tcatgacgtt gataagatag
    40561 aaaacttcta gaatctgtta acataccaat aaaattcgcc aacatgcctt gctctgctaa
    40621 ggcattcatt tgactaatca tccctaattt agtatctgca aaccaccaac ctgcaccaaa
    40681 ttgaaggtaa gatctcactc caagttcgtt agcttgaaaa tttgccaaag tgttagctac
    40741 tgctatattg taagctggat tgagattata ccaaatcatt ttaggcagac tatctttttt
    40801 aaccaaggaa tccaataaac ggttcatatt aacagttaaa gccacttggt caccaagtga
    40861 atccacaccc acgtcagctc ccaatttttc aaaaatagta gaatgattat ttctcaaagc
    40921 tccaaaatgc acctgtgtta caaaaccata ttttttatat aatcggcaaa gttccataaa
    40981 aacagctgtt tgccatttgc tgatttctga ttgatttggg atatagcctt cacaaacttt
    41041 attaaataaa tcgttcaact ccaattcatc agtttgttca aagacgattt ccgtaaaact
    41101 gatatcgcta gccctacaac cattttgagc aaaatacgca atgcgttctt ccattgctgc
    41161 tataaatgtt gaaaaatctg taatctcttt ttgagtaatg tcacccagtt tagtaataaa
    41221 atcaacaaaa tgtcgatgtt caataaaagc ttcatctggc ctaaatgtcg gagcaacaac
    41281 tgttttaaaa ctctcatcag cagccaactt tttatgccac tcgagagtat caagagggtg
    41341 gtctgtagta ccaataaaca taactttgct atctgcaatt aaacgtctag gacttatttt
    41401 atgctctttc aagaaatgat ttaaacgatg ataaatttcc tcagcatttg attctgtcaa
    41461 aatctcatta acaccaaaga tatttttcaa ttccatagca gaccaatgat aaacaggatt
    41521 accgtatgct ctttctagtg ttctagcaaa tgctttaaat ttctcaaggt tggatgctgg
    41581 acctgtaatt tctgcttcag aaataccatt agccctcata agacgccatt tatagtggtc
    41641 accaccaagc cacaaatcca caatattatc aaaaatatga tcttcaaaaa tgtcctttgg
    41701 atctaagtgg caatgataat caaaaatagg ttgtctcttg acctcttcat acagctgtat
    41761 agctgcttga ttttttaaca taaatgtttc agtattaaac gccatattct tctcttactc
    41821 actaatcaag tgcagaaaca aactgtttag ctattttagt aacactatca tagccttctg
    41881 tggctacttt actagataat gctgagccaa caccaaccgc aactgctcct gcttttctcc
    41941 attcaactac atttgaaaca gagacgccac ctgatggcat taaatcaaca tctggaatag
    42001 gaccatgaat atctttgata aaacctgaac ccacaactcc acctggaaaa agctttataa
    42061 tctgacattt atactttcta gctaccacga tttctgttgc cgttgcacag cctgggaaat
    42121 aataaacctt gttttcatta gctaaattag caatatctga atcaaagtgt gggctaacca
    42181 aaaatttagc tccagcatca atagcctctt tagcaagttc agttgtcatg accgttccgg
    42241 cccccacaat aatttcttta ttatttttaa agtcatctga taattgtttg atgactttgc
    42301 ttgcttcagg agtcgaaaaa gttacttcaa tattccgaat accacccaaa atagcatgct
    42361 ttgcgatttc gagcgcatcc tcagatgatt tccctcgaat aacagcaaag aaatagttgt
    42421 ttttcaattg atttaacata atacctccca aaatgtaacc gttatcttta actatattat
    42481 ataagtcatc tgatgaattg tcaagaagaa tactaaaaaa atagctattc aagaatagct
    42541 atttttcttc ttatattaag ctttcaacgc tgaacgtcta acactcatga tatgcataag
    42601 catagcatca tgtgcagcta ctggattttt ttctcggatt gctttgataa tttctctatg
    42661 tgcttctaaa ctatccgact tcatctgacg atttgtataa ttcgcattta tcagatgaat
    42721 tgactggttg atgactggca aaaggctatc cattgcaata ttaccactgt atttagctag
    42781 catggaatga aattccacgt ctaaaagtaa atgtttcgga tcgcctgcgt gaacagcatc
    42841 ttcaatatct ccaacaagct tttcgagtaa ttgtaactga tcatctgtaa ttctataagc
    42901 taccagctct gctattcgag gttccaataa aaggcgtagt tcaaagagat ctgatgtcaa
    42961 cctatctgtg tcctttatta gagagaaccc taatggatcc tcagaaacac cctttttaga
    43021 actaatatat gttccagagc cctgtctgac ttctaaaata ttacgcgttg ctaaacttct
    43081 gactgcttct cggattgtgc tgcgaccaac atctagatct tctgctaatt catattcatt
    43141 ggggagctta gcaccaacag ggtattctct ttctaaaatt aaatgcaata gacgatctgc
    43201 tgtttgttct actaagggtc tagccatatc atcacctctt ctaaaataac tacccctatt
    43261 ttacactatt ttttttatta tggtaatgag gaatagtcat ccagcgtttt ttaaattctt
    43321 tgacgacttg tttcggctgg cgattccttg aaaagagacc tttgtgattc ccctgtacac
    43381 gaagtatcat cagattagtt tcaaagtctg caaaattcca gacttgctca ccaactaaat
    43441 taggaatacc atcaaaaaca cgatgactca tttcataaaa atcacattga aattcttctg
    43501 tatatggaat attccaagta gaatgtaatc ccggtaacgt atcagcgcca tactccgtta
    43561 tgataattgg tttgtcagga aatttatctt gccattctaa taactctttt cttataccta
    43621 cttctgcatt tgttaaatca ccgtggtcaa cgtaccaacc atagtaacga ttaaggcaga
    43681 caacatcaac caggtccatc acttggtctc tatctggggt tgccattaaa atattaacca
    43741 aggtgacagg acgtttttga gggtctaaat ctttataaag ttttactaat ggctcaaaat
    43801 aatcatgtgc tcccgcttca tgactagccg gttcgttagc gactacccac atcacgacgc
    43861 taggatgatt tttatcacgc ttcactaatt cttggatagc ttgttcatgc gccgcttttg
    43921 tttgcatcaa attccacgta ccattatctt ttggtgacaa atctaaggaa gcgttaaaat
    43981 tttgaaataa ccctactgct ggaacctcat ctatcactaa cactcccatt cgatctgcta
    44041 aacgcatcat ttcttcagaa taaggataat gggatgttct aaaagagttt gcccccatat
    44101 ccttcaaaag attcaaatcc attaagttag cagcttcatt caaacctctg ccattaatga
    44161 acgtatcttc atgtttacca aatcctttaa aataaatagg tttgcgattc aataggaatt
    44221 gaccatttgt cacttctatt tctctaagac caaaattttc ctcatagaca tcttggagtt
    44281 gattatcaac aaaaatttca acacgcgctg tgtacagata ggcatttaaa acttcccaaa
    44341 gatggacatc actaagaaat aatctgctat ccttcgtttc acccactagt ttgttatctt
    44401 catcaaaaac gctaattcta acttcgtcaa ccttttgatt agtctccact aaaaaatgta
    44461 aatctgctga ctgtaaatca tctgatagac gacttgtaat tgtaatatca aaaatatgat
    44521 ttttaggacg tatcatcaac ttaagaggac gatgcacacc tgcataattg aagaaatcaa
    44581 aattctcacg tactttcttc ttaatgctac catcttcttg aatgatttca ctataatttc
    44641 cgaccggaag cgtggtatag tctaaaacgt tattagcaca aattgatacc ttaattttct
    44701 cattattata cttacattca ggaacaagaa cttcaaaagg agtaaaacct cccttatgct
    44761 ctcctaccaa tacaccgtca acataaattt tagcttgatg tgtcactgag ccaaaacgca
    44821 gcaccatctc ttcatcctct gatactttag gtacttcaat gactttttca taccaaaaat
    44881 cacctatata atcacgtttt tctttactta ccatcaaatc attaaatgaa gtcgggataa
    44941 ccatgacttc atctgaaggt agtaattcat ttggattatg ttctcctaat ttaaagttcc
    45001 aaataccgcc taaatcataa gtatttcttg tttttgtcaa taatggatat aacatacact
    45061 ctccttttac actatcgttt aatatcattt gtttcttttt caatgaggta agcaatatca
    45121 tctcgtgtca tgatattgat atctccttga atggtatgtt tgtatttaaa tgtagccata
    45181 gctatttcta aggccctttg aggagtttct ttttccagaa gcgcatgaat taaacctgca
    45241 gcaaaggcat ctcctgtccc cacacgatct aatacttcaa cgccagtttt atcagtttga
    45301 tataacatat tattatgata caaatatgat tttaacatat actgattagt atattccatt
    45361 tccctttgag taaaggcaat atagtctaac ccatattctt ggacaatctt ttgggttatc
    45421 ttttcaagta attcaatatc aaggtaaggt ctggataagc ccaactcatc ttttaaatct
    45481 tcactttcgc cagctaaata aatcggctct aagccaaagc aaacatctaa taatcctaat
    45541 agtggcgata attgctcacg tgcctcttga aaagatgacc ataaactctc acgaaaattg
    45601 agatcaatcg atacttttac acctccttct ttagcttttg tcataaggaa ccgagtcacc
    45661 tcatataagt ccttggttag tgctggagta attccagaaa catgaaacca atccactccc
    45721 ttaaaaatag aagtaaaatc ataatcactc aaggtacttt cccaaaaaga tgaatagtta
    45781 cgatcgtaga taaccttatt tgttcttacc gaaaatcctt gttcatagta ataaattcct
    45841 agtcgtcctt cttttttaat tatagcagct ggactaattt gctgtgacaa tataaattga
    45901 cttgccatct tgcccaaatc attatcaggc aaagctgaga ccaatcctac atggtaacct
    45961 agttgagcaa ggcttgctaa gacattaagt tctgaaccac cgaattggca ttttaggtga
    46021 ttagcctgca tcagtgtgtg atattgaggt ggagataatc gcaaaagaac ctcacctaat
    46081 gaaataattt ttgccataag aatcctccaa tggtagctct tatttaacaa aatagctgct
    46141 ctattgaaga gcagctaaac tttttaatct ttatcaatag gatattttgt tacatcaaca
    46201 taatccatat ctgtcaaagg aacattacga gcaatatctt tagcacgttc cttagtagtt
    46261 gctgctttca taacttgaat cttctcttga atacgaacca tttcttcttt atctagtttg
    46321 taaaatttca ttaaaagaag agctatagac aaagctataa atggaatggc cacaaggata
    46381 ctaatagctg ccatttttaa atcagggggc agtggtgttt cgatagttgg aaatgatttt
    46441 gaaaatccaa ttcctgctaa cacaaaacct actaccatag gagcaaatga agaagcaatc
    46501 gaatctgtta aggagaagat agttccaatc attcctgata cataacgacc ggattcagaa
    46561 gtttcatagt cagaaatatc tgctcccata gtcaatacta aactagcagg tgcttgtgaa
    46621 gcgtatctcg caataatatt agtaacgata aataaaatag tataaagatt taggcttgtt
    46681 aaactaaggt caccaggctt accaacgtaa agaactgcac caaaagcaag taaaccaatc
    46741 ataccgatct gaattgctct gacatatgaa aaacgaagac cttttttacg agcaattgtt
    46801 gagaacaaaa tattaataat aacaccagga acaatgaaaa gaagtgagaa ttgtcctgat
    46861 aaagcataat taccaaataa aataccaaac aatagcacca taacaacgga atcgccaaag
    46921 aattggatgg caaattttac caaagctgca gcaatggata atatctgaag cggtttattt
    46981 cctttcaaca ccttccagta atcttttaga gcagtctttt gggtattctc tcctaaacca
    47041 aagaattctt ttctgtcttt tgcccaaatc ccaataattg caacgattgc taaaatagct
    47101 gaaataagaa ttgttccaaa aataaggaca ttgaagaatt gaggtgtaaa attaccaaat
    47161 tttggtacta agaaaactga aacgacaaac tgaccgcctg tcattaatga tgttgtcata
    47221 actgcatcaa cgatattaaa aataggacgt tgcttagggt catttgtcag tgctgtttgc
    47281 cctgctttag ttattgtctg ttgcattgag tatccaattt tatggataat tagaacaagg
    47341 ataaataatg ggaaacgaat attttcatcg acacctctca atgcaagaag aaaaatcaat
    47401 gacaaagccg taataacgtt cccaataatc aaaataggac gatacttacc aaattttgta
    47461 tcagttttat caatcataat accaatagct ggatcaataa aaccatcaaa aatacggata
    47521 taacccataa tttgactgac aaaaatggct gccgagccaa gaacgccagt tgagaaatag
    47581 gtaacaaaca taaacgtaaa taagtatata tttgtagaag cattgttcat ggcaaacata
    47641 actaactgcc atactttcgc tcggttataa gatacaactt cttcacgttg ttccatatcc
    47701 attgttttct ccatgagaga accctccttg tattttgtaa acgcttttat gattaaataa
    47761 tactattcat ctgatgtttt tttcaatatt tttataaaaa atatcttttt tattataaaa
    47821 aagactttcc agctataaat acataagatg tttttttgct ttaaaggtca taatcatcat
    47881 atgttttgtt accccacaaa aaaagatgcc taagcatctt ttagttgact ttcgtagtag
    47941 ttgttcccgt ttcaatcact tctacacaag catttaaggc accctctact agatttttga
    48001 ccgcttcatc tgtataataa gccgcatgag gagtgtatat tactttcgga tggttaatca
    48061 gtcttaggaa atccttgtct gaaatgtctt gaccttgaca atttttaggt atatatggcc
    48121 cctcaaattc ataagtatca ataccagctc cctccaaaag cccttgatct aaagcctcaa
    48181 gaagatcttt ggtttccacc aatgctccac gtgccatatt cattaaaata gcaccttttt
    48241 taaactgttt aaacatatct aaattaaata gatgcgtatt ttcagcagtt ggtggcatat
    48301 gtaaggacac aagatcagct tcttcaacag cttcttctac cgaattgaca tactcaagga
    48361 taccatctgc cattggatta tggtaaatat cataaccaat aacacgacat ccgaaccctt
    48421 taaaaatttt agcagtcgct agaccaatgc gaccagttcc tattatagcc acagtcatgt
    48481 tacctaggac tcgtcctcga attggcaagg tccatgaaaa attctgttca cgaacattag
    48541 ctctaataag ctcaactttt cgaataaggt taagagcgat tgtcactgta aattctgcaa
    48601 tagactccgg tgagtaactt ggcacattgc taatgataat accatgttgc ttagctaact
    48661 caagattata catatcaaca ccagcactgc gctgagcgat ttgcttaatt cccatttctt
    48721 tgagaagagg ataaatagca tcatctaaag gctcaatctg tgcattagca atcccatcaa
    48781 aaccttctac ctcattaaca gtttccaatg tcaaaggtcc ttcactcata gaaagttcca
    48841 catgatttcg gtttgcccaa tcttgtgcta atgttgcttc ttcttctcta acattaaata
    48901 ctttgagttt catcactaat atctccttaa atatctaatt tatagtccat tggatcaata
    48961 attttaagta cttttataaa gaggtaatta aaaataaaat tcaatataat tggtgcagca
    49021 acaaatatta taattatcaa taacatattc ataactgacc atccaccttt tgctaaatta
    49081 agagcattaa tcggtccaat caaaccactg ataccaaaac ctgcactagc tggtgttcct
    49141 tgaatattaa agagagctcc gagaattcct agtattgctg ctgaagatat cataggtagc
    49201 ataatcttcg gttttgctaa tacattagcc atcgaaatct taggagaccc taaaacatgc
    49261 gctaaggctg tccccttaga attgactgac cacccagcca tacaaagccc aaaactagca
    49321 gcacagatac ctaaattagc agcaccagaa ccaattccgg acaaactaat tgccaaagca
    49381 atcccaactg ttgtgattgg agatacgatc aagaaacaga aaatcatcgc aattaaaatt
    49441 gacatcaaaa caggttgtaa accaagtaat gaagcaatcc cttgtccaat catggtggta
    49501 atcattttga cataaggtaa taacacatga cctactcctc caactagtaa gagtgttact
    49561 gttggaatga caatcaaagt gaatgatttc gtcttgtcag ataaaaattg gatcaaaagg
    49621 acacctaagg cagcagtaat tcccatgtta atgatatcgc ctgttccttt tagcataata
    49681 gcgcctttaa ggaaggttgc agctccacct gcaaacataa ccgctaatcc tagtgatgct
    49741 gattgaatgg gattaaattt aaaattgaga ccaaccataa taccaataac aagccccatc
    49801 atacttgttg caactgctgt tgcagcgatt aaagttgcaa agccgctcca cattggcagt
    49861 aaagctttca ttaattctcc aaggattgct cctggtatca agacaataac agtccctaaa
    49921 gctaaaccat ttaatacgtt cattataaac gatttagttg tttgtttttc gacaacatcc
    49981 gacataagat gcctcctact aataatgatt gtttgtgatt tcactaacta tattaactat
    50041 atcatagcta tttgcaaaag gaaaattgga aaaaattata taagtatagc ttttactaat
    50101 attattgtgg aaataatcat aaaggtcaga ttgatagact tgtgctatac tattactaag
    50161 aaaaaaggac agatattatg gatattagac aacttactta ctttattgct gttgcagaag
    50221 caaaaaatta ttcacgcgct gcaaaaagtc tttttgtgac ccaacctaca ctttcacaat
    50281 ccattaaaaa attagaagca gaattaaata ctattttatt cttacaaaat ggtcgtcaac
    50341 tcgctttaac tgaagcagga gaaattctct atgagaaagg acaattgttg atgactaatg
    50401 ttaatcaaat ggttacggag atccagcaat taaatcaaga aaaaaaggaa ggtatccgtg
    50461 tagggcttac ttctcttttt gctattcaat ttatgaaaca gatttcaact tttatggcta
    50521 cacattcaaa tgttgaagta tctttgattc aagatggttc ccgtaaatta caagaattgt
    50581 tagcaaaagg aaagatcgat attggcttat tgtcctttcc aagtacccgc aatgatatca
    50641 ctatcgaacc actgcaaacc tcaactaaag gatataaagt aagcattgtc atgccaaaat
    50701 cgcatcctct tgcaacactt cctgaaattg aacttaatga tttaagagat tataaagttg
    50761 cctcactcaa cgaacattat atgctaggag agatgttgcc tcgtaaatgt cgttctttag
    50821 gatttgaccc ccatatcgtc tttaaacaca atgactggga agttcttatt catagtttac
    50881 aagacctcaa tgcagtcact attttaccaa gtgaatttga aagcattagc caagttcaag
    50941 atttatgctg ggtcccactt aaggataaaa ataattttta tccgattggt attgcttacc
    51001 gaaatgatac ttcttttagt cctatgattg aagaattttt aagtctttta aagaccaatt
    51061 aatagcagtt aaaccaaaaa gttttaaact ttttggtttt atttattttc caaatgacaa
    51121 agaaaatcat aaactttttg attccactca taaagtgttt cgttacagta atctaaataa
    51181 taaaccctgt gtttcaagtc attgggcaca aggctttgat aatcttcttg ataatgtttt
    51241 gggataacac atgcaactaa atgttctgct aaagaaagct gatttttaat tttagcagga
    51301 tgataaccat caaaaataga atcaggatga ttgatgatat cttgataagt aaaatgaaaa
    51361 ctgacccctg gcttaaaacc tactgttaac tcctgttcag atggtgcttt gcctaacaat
    51421 ctttccatta ctaaacgata accagaattg gtatttgacc agttcaacat cacatagtca
    51481 aagtaatctt tagggtctcc agcagcattc ctcttatctt ttaccagtac ttcagcaggc
    51541 ttattaaagg ctttaactgc tgataagatt ttaccttctt taaaaattag ttttgcagtt
    51601 tcaatcgtac atgtatggct taaaaaatca gaagttaaac taataccatc ctcattaaaa
    51661 gtagctgccg cttctctata tttgggaata tagggaaaag taaaactatt gtattttaaa
    51721 aaatgaacaa tttttgcgag gagatgttta tcgtcacatt gtatctcagt ttttctttta
    51781 tattgtttct cgtaagctat gaaagctcct atcctttcca attcaaacgc cttaattcga
    51841 ggataatcca gcaaagccaa ttggtaaaca cctccccatt gtaaactata tgctgcacga
    51901 caaattttta aaatcatttc ttcctccttt tgataatccc tacaacaatt tctattaaat
    51961 agtagcattt aaacgtaatc gactcaataa cttggtcaat tttcgatcaa aaataagatt
    52021 aagataccaa aaaagcctgg actatactta ccagactaac atactgttta ttaaaatgaa
    52081 tcaatctttt gttactacta attgcttacg attattaaag aaaattttat aagccaaata
    52141 agtagctaca aaagaagcta tcgaagtcgt tgctaatagc ataaacgtca ccatcatttg
    52201 gtatttaacc gcttggatag gtgaagttcc agcaagaatt aaccctgtca tcatccctgg
    52261 caaagataca atcccaagtg ttttagctga atcaattgta ggtaccatac cagttttgat
    52321 aacatcacga ataatatcaa tcgaagctgg caaaatatct gctcctaaag ctaattttgt
    52381 ctccacttcc tcttgtttac ttctaaattc ggacaataat tgtttatagc acaaaccgat
    52441 tgctaccata gaattactaa taatcatacc acctacagga atcatttgat tcggaacaaa
    52501 ttttaaaatt cctgaaaaaa tcaagacaga taaagtgatt atagtccctg acccaatagc
    52561 tataaatgaa ataacaaacc ctttattaat ccctttacca cgcttagctg cgttataaga
    52621 agcattaata atcatgaata gcattaaaag agctgtgaaa ataggatttt gataaccaaa
    52681 tatatagtct agcaaaaaac ccactgctaa taattgaatc actgctcgga tagcgctaat
    52741 aatcacttct ttttctaggt ttaacttttg ccagtaagaa aaaaataaag taatcaaaac
    52801 taatgacgat gccattagta atgacattaa agaaatagaa tttgcaccgc tcattttata
    52861 acctccattg cttcaatact accattgaca acttttaaga ctttattggc aaatcgacga
    52921 ctctgttcct catcatgcgt aatccacaaa atcgttattc ctttgtcatg taatgattta
    52981 ataacctttt caacaatagc tttattatga ttgtctaaag ccgaagtaac ttcatctaag
    53041 agtaaaactt taggttcaaa caataattgc ctaatcagag caatacgttg cttttctcct
    53101 cctgatagtt ttttgacatc ctgtttcagg taagactggt ccatttcaaa acgttgaaat
    53161 aaatcatcaa cgcgtctata atctactttt tcgtgtcgaa tgtggtaagg aaaactaata
    53221 ttgtcttcca ctgtattacc aaataaatgt ggcgtttgaa aacagtaaga aatcattttt
    53281 cgactttcaa tcggttctaa ttggttcaaa tcttttccat caaagaaaat atgacctgca
    53341 gtaggactca ttaaaccact ggctaatttt aatacagtac tcttaccact tccagatggc
    53401 cctacaatag aaacaaaatc accttcatca attgcaaagt tgatatcatt taaaaccaac
    53461 ttatcatccg ttttaaaatc aacgtgttta aaggttaaat aggacattgt taagctcctt
    53521 tatttcttat ttataatcat tataattaag tctaacataa aaatcaaaaa aaagctcttt
    53581 agaataattc taaattaata agtaaactta ttttacctct tattaaaaat tttctgataa
    53641 aataggcttt gtaagaggcc ttataaacaa gatagttatc atccaagtct ctgcacttta
    53701 gttgatcaat aaaggagact cctatgtttt taatcgattt tattttacat attgatactc
    53761 atatctatgc tatggctaac actgttggta attggaccta tctattgctc ttcctcgtta
    53821 ttttcgttga aactggggct gtcatctttc catttttacc aggtgatagt ttattatttg
    53881 ctgctggagc ccttgctgca aatccaaaaa tgtccttcaa tattgtaact tttcttatta
    53941 ttttctttat tgcagcattt attggtgata gttgcaactt cttgattggt cgtacatttg
    54001 gttaccgttt tatcaaacat cctttctttc gtcgtttcat taaagaaaaa aatatccgag
    54061 atgctgagct ttattttgag aaaaaaggta ctgctgccat cattttaggg cgctatatcc
    54121 ctattatcag aacctttgtt ccttttgtag caggtattag tcagctccct ccaaaagtat
    54181 ttatcaaacg cgccttcatt gcagcattat cttggtcagt tattgcaaca ggttctggtt
    54241 ttctctttgg taatattcct tttgtgaagc agcacttctc gcttattatc ttgggaattg
    54301 tatttgttac cttaattcct gtccttattt ctggtgtcaa atcatatcgg aacactaagt
    54361 aatgtatcca aaaatttcta tttaacacta ttaatcacta agaatgacaa caaaaaaaga
    54421 gcaattagtt gctcttttat taatttagtg ttatttctcc tgttgcataa tccatagtgt
    54481 atgatgcttg tgtgtttgga atagcaacat taaattctaa tgtgccatct tgtgatttag
    54541 cttctaggtg cattgcaaat ggaactaaat cagtatcatt acggtacaat ggagttacac
    54601 ggtaacgaac acgtttgttt tggtcaaccg ccttacgaac taagctttca taataatttt
    54661 gtccacgatt gattttttga tttgattggt tggaatgagc tgtttgtgtg acaacatttt
    54721 gaggatttga cacggaagca tcccaacctt tgaaatttcc agctaaagca taggcaatta
    54781 aatgcccctt gtcgactgca tgcccataat ggtcattagt agctacttga tgccaaccta
    54841 atggtttcca atttgcatcg ggaagagtgg tatctctgcg attttgatat tgacgaatgg
    54901 ttctatcaag cattgcatta gcagttgata gtctttgttg attgtcaatt gtattttgaa
    54961 catacgcttt aacattgaca tcagcattta aatttgtctg attattattt aattcataaa
    55021 taccattaac atattgaaat ggtaggttac caatagcatt ttttaaatta ttttgaaatt
    55081 cctggctcgt atcaacagat gaattgacat cagcatgtac tatcggcgtt acgttttgcg
    55141 gctgcgttgg aagaccaaag gtcattaccc ccaacatacc taatgtagca attacggtta
    55201 taaacagttt atgtaatctt ttcataagct ttacctcttt ctaattacat taaacaagcg
    55261 tctaccatat tgatatccgt tttagctaat tgctctcctt ttgtcacaac catatcatat
    55321 gcctattatt taagcatgtc aatcatttat ttaatcttat attaatttaa tattacttta
    55381 aatttaattt actcgttaaa ttagaaaatc tgcaaataat agctttgttc atcccctaac
    55441 ttaaatcaaa atttatgcta tgatagtggt aaggaggctt gttctgtgaa aaaaacaatt
    55501 ggtattatag gtatgggagt tagtggttta gctgttctac tagcactttc tcgtaaaaaa
    55561 gagaaagagt taaaacagtt agctattttt tgttttgatg atactgtcca ctttggacga
    55621 ggaattcctt ttcaagaaga cgttgactca gctttaatca attccccaat caacgatatt
    55681 tcatttgatt atcgccatat gtctgacttc atggattggt tagaagaaaa ttactatgac
    55741 acaaatctaa cctatgtatc aaggacactt tacggacaat acctttcaga aagaactaag
    55801 ttacttttag ataaactccc tgtcacggtt attaaaacaa gagtggataa actaaactat
    55861 ttatcagatg agcaacaatg gcaagtctat attcaagata agctttttcc tgtcacattt
    55921 gatgaattac acttagcttg tggtgctctg cctgttatag aaccttatca tttggaaggg
    55981 aagaccgatt atatcaaaga cccttatcct atcaaatact tggctcagga aaattggaag
    56041 ggaaaaacag ttgctatcat cgggactggt ctagctgcaa ttgatgttat aaaatggttg
    56101 ctactcaata cacaatcttc tgttaaagca ttttcaagaa ccaactactt ccctagcgtc
    56161 cgtattgtta atgatttacc acttacttgg cactttttta ccaatgataa ccttaaagca
    56221 tttctatcac atccttcaaa aaccttcact attgccgatt ttaatgattt gatgtctggc
    56281 gaattggagc atcttggctt taaaaactgg aaaaatacaa cagaaacttt tttatcccca
    56341 ggaattgatg gtctcaagtt agcctttaaa taccctaagc aactctatct attacaacaa
    56401 ttagcttcac gaatcactgt ctggttaact gacctttggc cactcatgtc attatcagat
    56461 aagaaagagt ataaagcact ctatggaaaa gcgattatta acttgcgaaa tcctatgcct
    56521 gatgaatctg ctaaagtaat tttgaaagct gcacaaaaag ggactttttc tctgatcaat
    56581 aacgtcaaga caattacaaa aaatgacgaa ggattttcaa tagaaaaaga aaaacaaaat
    56641 catatatctg tcgatcgcgt cattaacgct actggttacc agttaaacac taaaaactgg
    56701 acaaaagcca atccacttct aaaatcgctc atcaatcaag aattttgtca aattgacgat
    56761 caaggaggga ttactatttt aactgaaaat gcacaagttt tgtctccaaa atatggtcca
    56821 atgccaggtt tatatgcgca tggtgcactg gttaatggtg tcgtttatca gaataactct
    56881 acaattaaaa ttcaacaaat ggcagagcga gccttttctg ataataaata accatttcta
    56941 aaaaatacct cctcttttat tatttaagaa agggaggtat ttttttatta taaattcctt
    57001 tgttgtaata tagactgtgc tatactttct tttgataatt cttttaagtt atctcctatc
    57061 aattgtaaag cagtatcagc catatgagcg atctctctac tatgtgtcac cataataaca
    57121 catcgttttt ccttatgcgc aaaatcttta agacgcatcg taatatcacg cgcaatgtca
    57181 aaatctaaat ttcccgtagg ttcatcaagt aaaatagctg gtgttcctac aaccaaagct
    57241 ctcgcaatag ctacacgttg ttgttgccct cctgataatc tcaagatatt tcgcgttaac
    57301 atatcttctt ttaaacctaa atccaacaat aattgtttat ctgcagtagg ttttaccaac
    57361 tgaacatttt ccaatggggt tagatagtct aaaaggttat aattttgaaa gaccaaagct
    57421 atattctttt tgcgatgctg agcataacct gcttctttaa tatctttttt gttaaataat
    57481 acttgtccct cagtaggcga atcaagacct gccatcaagg atagaagggt tgttttccct
    57541 gaaccagaat tccctaatat agtataaaat acccctgact gaaaactata attgatattt
    57601 tctaatgttt tattgtcgtt ccccttataa cgataagcaa tattttttaa ttcgagcata
    57661 gatttctctt tcttgagcta tgtattcaat agcagagcat attcatttta accgttaata
    57721 tagatgtcac tattggtcgg tatacttatg gcactttagg acagttttga taagattccc
    57781 cttggtttgt agcgagcaat aattaaacta gtagtaataa tagcaaataa gacgattgct
    57841 aaaccaacta gaattactaa ccagacttcc tcaggcttca cttgtacatt taacgtctta
    57901 atcgtttgca taaacatatc tgtatcacta tcagccccaa gctgcatatt cccaaaccct
    57961 ttcataaagt ctgctctgac agttctagag gcctgataca ctaaggagtc tgctaatttt
    58021 tgaccaataa attgactgat aagtactgat aaagcaaagg ctggaatagc aatcattagt
    58081 acttctactg cgtactgaca atgataattg ctttagactt accaatagct aataaaatac
    58141 ctgtctcatg ggttctacca ccaatccaga atagcagagc catcatcact aggataatag
    58201 atactactaa catgccgcct tgtagagaag taattaattg attcaagaca tctatacttt
    58261 ttgcataact tgttagcaag gttgctgtaa ttgtcagttg atatttattc caatcaatag
    58321 gattgttttt tgataaaact tttaagagtt tctatatcag cattcttttt aggatataca
    58381 gttgtttggc gataaaaggc cttaccttta gaatagccgt ataaatcctt aatcaattgc
    58441 gtatctgaaa aggctgtatt ttctagcatc tcataagggt aatttgattt ttgtaccgtt
    58501 ttacctttat atatgccaac aatagttgct tcaactaatg ttttatcctt actgtttcta
    58561 acggggtctt ttgaaaacat taacttatcg ccaactttaa gatgatttgt cttcgcaaaa
    58621 gcctgatgaa caattataac tcgctgatct ttcggcgtga tatgtcgccc tcccttaaga
    58681 gaaatcatcc ccgctgtgta tttaggatct gtatcggagt aactattacc atgaacttgt
    58741 aaaacttgtt ctttatattg ttctttatca tttggtcgtg taaccatttt ttccgaggtt
    58801 agtccagcat aagccatcaa attattatta acgcgcttaa catttggatt acttgctatg
    58861 ttaactattg ctttttcagg gatttctcca gatccgtaac catcaccaac aaggtcagta
    58921 ctatccgatg aaatatgcaa aattgatcca gtttgccttg aaaatgtctt cttagcagta
    58981 agaccagcat tttttaccgc aataccgcta attaatgtag aagcaactag ccataaacat
    59041 acgaataaag tgatactctt acgcttttta cggctaacat ataaaaagga acgtttaaat
    59101 aaattcatat taacctccta tgacattcgt gacaatattt gctttggttg tagtttaaga
    59161 atagaataac tagataatac aactgcaaat aagcaaatta aagcaccctg agcatagagt
    59221 ctaaagacat ccattactcc aacagaaaca gcgaggttat caattctatc aataattaga
    59281 ctactatttt gtacaatttg tgaactcaca ccaccattaa cttgtgataa aaggccagct
    59341 gcaaatgttt ttccaagaaa gacaccacca ataatagata gtgtgaaggc agcacctgct
    59401 actaacagta cttctatcag ataatgacct atgatttcaa acttagtttt tccgatagcg
    59461 agtaaaattc ccgtctcttt aatgcggtta ttaatccaaa atgttagcat gacaactaat
    59521 attgccatcg ccacgagact aacaataaga atgattcgtc ccactagacg atgcaatgtc
    59581 tcgatattac gataagaatc tccataaaac tctttagctg tttgaacatc taatttcccg
    59641 aaatcaagcg gccattgttt gatgttttgg ataactgtat ccactaaacg tttatcactt
    59701 aagcgcactg caaccgtctg ataacctttt tgtttactaa tcttcctaac caatgttgcc
    59761 tgagcaatca atgtattatc aatattggga ttaaaagtat ttttagactt agcactattc
    59821 gcatcataaa ttcctgcaat agtaacattt tctttgccta atctcaattt gttacccaca
    59881 cttaaatgat tcttttgtgc taacttcaat ggaactagta cttgttttgt attatcggcc
    59941 gctaaatggc ttcctttctt taagaccaat gcttttgtct gaaaagctcg taaatcttct
    60001 gtactttgat aacctgctaa tgtaacttga ttctcttctt tagaagagtt atatggtgat
    60061 acagatttta aacggctacc aacatcaatg cttaaacttg cctctactga ctcaaccatt
    60121 tttgcttttt tcaaagcttc gtatgcttgt tcaggccaat aagccttttc tttggaggtt
    60181 aaataaatgc ttttcccttg ttttgttagg atattttttt ccaaagaatg ctgaatactc
    60241 atcagactga ttaaacatgt gcctatagtt agaaaaacta agaacaaggt tactgttttc
    60301 attttttgac gtttcaaata agctaaactt aatgtccata ggttcactat tttccctcct
    60361 ttttctatct taataatctc tagaataacg atagaagtcg ttagatattc tctatcgact
    60421 tctacgttat ctatcaatta gtgattttat agcactgttt ctaaaaacaa ttatttctca
    60481 aatgttttca taaattggtt agtataatca attacttctt taaggttagt atcataaatt
    60541 tggtgagttc cgtctagtgt atgaatagag acttcttttg gatcttctga taactcctta
    60601 tgttgctctt gccaactgcc tttaattggt atatctaaac tacgtaattg aagccctaag
    60661 tcactttcac gtgctaataa atacatagca ggaattccct ttggaatctt atagttggca
    60721 ctttcgttaa tcatattaga agaattcttc gcttcttctt tcatattaga attattaagt
    60781 atcttatgtc caatagcaaa aaattgtgca ttaatatcgt cggatacttt aggaatttta
    60841 ggtaattctt ttaacactgg agcagcgaaa tcaacttgct taccaccttc catacccggc
    60901 gttgatgtat ctaaacctat taatcctagc acttcctttg gataagtact gatgtattta
    60961 agggcataaa caccaccaat tgaatgagag gcaattacat attttccggt aataccaact
    61021 ttttgtaaag cttcgtggat atctgaggta atattagcta gatttcgtgg ttggtcaatg
    61081 acatcactta aacctgaacc aaatggctca actaccacaa ccttatgagt tttacttaaa
    61141 ttactaatca aattatggta ctgatgtttg gcagtcaccg tcccttgacc aggaataaag
    61201 acaattggta ctttaccttc tccaacagta tagacattca tcttcttgcc tttgacttca
    61261 accttttgac catattcttt gataggtgtt ttgatatatt tttgatattg tttgttacta
    61321 ataaagctct ctgtgccttt attatatttt tcaagttctt tcgttaactt cattcgcttg
    61381 gctgctgttg ccttgtcttc cttcggattt gcagttacgt tttcttgctt aggatcctca
    61441 gttttagttt tttgattatc tttattggca gaacatccaa cgcaaagcat taaagtcagg
    61501 gcgatggttg ataatgtcgt tattttattc atcttttctc cttagtaaaa taattggggg
    61561 gaataattat ttttcatgcg attgaatcca ctcgtcaata gccttcgcca tatgtttata
    61621 ttgcgtatga taaatcaagt ggctagcatc tagtgtctca acagaaacgt cttttggatc
    61681 ttcagataag tcaatatgtt gttgttccca gcttttaagc ttagggaata tttttttacg
    61741 catttttata tcttctgctg actcattagc taagaaatac ttagctggca tacctttagg
    61801 gaacttaact ttttccattt tatgaagatt gtcactaaca cgcctatctt catcgcgctc
    61861 tgttttatta cctgattttt tagcaccgat tgataagtat tgttcattgg ttttcttatc
    61921 aacttctgga atggattctg cccattttaa ggttgaagct gaagattctc cagttgtgcc
    61981 atcaccttcc atatttggtg tgcttgtatc catgccaatg aaacctttga catcgttaga
    62041 atatgtctta gcataattta gaccgtatat gccacctaac gagtgagcca taagatagaa
    62101 atttttgaca ccgacttttt caagagctgt atgaatctct ttggtaatat tttcaatcgt
    62161 acgtggctta tctgtcatat cactaagccc ataaccgagc ggttcaaccg tataaatttt
    62221 atattttttc cctaatctat caagcaagtt tttgtaagca taacgtggtg aaatttcagc
    62281 ttgaccaggg aagactacta gcggaatttt cccttgaccc attgtatata tgttcatttt
    62341 tttaccgtca ccgacatcaa ttttttgtcc gtaatcttca attggtttcg ctttatattt
    62401 agttaggtca atagcacctt tagcaatcat agctttttct gcttctctct cttgtttttg
    62461 cttagcgatt gacttttctt gcttaggctg ctcagtttta gttttttgat tatctttatt
    62521 ggcagaacat ccaacgcaaa gcatcaaagc gagggcaacg gttgataatt tcgttatttt
    62581 attcattttt tctccttgac ttaggtattt cattcaacga ttacttttga tgggattgga
    62641 taaatccatc aatagcttta gccatatgtt catattgtgt ttggaaaata ttgtgactac
    62701 ctttaagaat ttcaacggaa gcatcttttg gatcttcaga taaatcaata tgctgctgat
    62761 cccaattttt caaaccttta aaatctgctt gacgtagctt gattccctcc acagattgat
    62821 cagcaagaaa atacttagtt ggcatccctt tagggaattt tacattcttc aatttaccaa
    62881 gattatcacc taagcgctca tcttcatctc gttgagactt attgccgtta atttttttcg
    62941 caattgacag atactgttcg ttagtcttat catcaacatc tggtaatttc attgcccatt
    63001 tagcactttc agggtcatac tttgtttttt gttccccttc catccaaggt gtactggtat
    63061 ccataccaat aaatcctctg acttcttctg gataattttt agcataattt aaagaataca
    63121 taccgccaag tgagtgagct gcaaggtaga aatttttaac tccaatttta tttaggcctg
    63181 tgtgaatttc ttttgtaatg ttttctagcg tacgtggttt agtaggaata tcactgagac
    63241 catagcctaa tggttctact gtataaattt tatatttttt acttaaccgc tcaattaaat
    63301 tcttataagc atagcgtggc gaaatctcag cttgaccagg aataaaaaca attggaattt
    63361 ttccctgacc agtttcataa atgttcattt tcttgccatc accaacatcg atttttttac
    63421 cataatcttt gaccggtttt gccttatatt ttgtcaaatc aacagcgcct tggggatgca
    63481 tctcttgatc agcagcttct ttgtttttgg gttcttcttt ttgcttatct gtgagtttag
    63541 tttgtttagg atgatgagac tgtgttttag atgtagacgt gtttgatgca gcacacgccg
    63601 tacaaagtag taggcttaaa gcaacagttg atagctttgt tatattcttc atgcattctc
    63661 cttattgtct ggaaaacata gctttaccaa agtgtcaaac gtttttctgg ttttagatac
    63721 attgaatcgc cttttttaac accaaaggca tcataaaatt cttggaaatt acgtactggg
    63781 atgttagctc tcaattcata tggtgcatga acatctgact gaattgagga cttactttgt
    63841 tctttggttg ctttttgacg ccaaatactt gcccatgatt caaaaaattc tttatagttt
    63901 ggattttttc agtcttaaga gcttctagag atgccataac accaccatta tctgcaatat
    63961 tttctgctaa agttaattta ccatcaactt taccgccatc tgctttaagg ccatcccatt
    64021 ggtcaatcat agcttgtgtt tttttcttat aatgttttaa atcttcttta gtccaccaat
    64081 cgtgaagatt ccctttctcg tcatatttca taccattaat atcaaatgag tgtgaaattt
    64141 catgaccaat aattgctccg atagctccat aattttgact aactgtttta gttttatcgt
    64201 agagtggtga ttgaaaaatc gctgctggaa agactatgga attggtatta ggatcattat
    64261 aagcatttac agcattggca ctcatttgcc aatgttcacg ttgattagac tggttaaatt
    64321 cttcaaatgt tttcttgttc gataattttc tgtagttatc attgttttca aagaagcttg
    64381 ctttactatc aaattggtac tgacgataaa gatcaggata atcttctgga tagccaatca
    64441 ttaatctcat gttatcaagt ttcttaatgg ccattgcttt tgtattttct gataaccaag
    64501 tgttgttttt taaccgtgtt ttatagacat taatgatttt cttagccata tgttcgacgt
    64561 cttttttagc tgcctcacca aaatatttct taccataata atcacccaaa atatggccaa
    64621 aagtatattc taattgggtg taagcatttt ttctttcgat tcaatctgag tcaaaccaga
    64681 cgcaacattc ttaaaagcac tcgctgcttg acgattttgt tctcctagaa aatctgattg
    64741 atcaactagc atagaaatca tcatccatgc tttcatattg gctaattgtt tactatttat
    64801 aacatcatta acctgtttaa aataagtttt atcttctaca ttgactgcct tattcgttgg
    64861 cccaactagt ttttcaatca attctttaaa gtcaaattcc ttggcgtaat tgtgaaccgt
    64921 ttccatactt tcagggttat atcgccccgc agctgtctca ctttcagctg tgattttact
    64981 ttgatcaact tgcgtttttt ctgataagag tctatcaaat gctatagctt gtttaactag
    65041 ttttctatct tcaatgttac tttttccagc ttgtttaagc aaagccattg ctgaagtacg
    65101 gtaagctgat aatttagcct caccttcttt attgcccttc ttatattggt caggtgattc
    65161 aagtaatgcg ggtgcttgac gaagcaccaa ttgcttttgg ctattatctc gagcattggt
    65221 ttccacagtc aaaccaaatg gtaaaacaaa accactcatc acaaaatcat gggccaaact
    65281 ttgaaagtct ttcattgaag agactgcttc aagtttttgt aaaactggtt ttagaggttt
    65341 gagaccattt ttatctcttg ttttaaagtc cataccttgt ttatagtatg caaccatttt
    65401 tttctgttca tcattggttg tttcaatctt tcctgaggcc attttagcca tatcagacac
    65461 taacagttgc tttaatttat cctcaatttc tgaaaaacta ttaacactag tctgcccttg
    65521 tttgagtttt gtattggcta gccaattatc attgacagcc ctgtagagat tttctctcgg
    65581 actgacatta tttgctttct tctcactggc agatactgca tgtaacatag caatatcttt
    65641 aaccacatat cctgatataa gacaagctgt taaagtaaga acactcatgg attttaaata
    65701 ggattttctt tgcataaatg acacctctct ttcttattca cttttagtat agcaagtagg
    65761 gtgttaaatt cttgttaaga attcactttt ttacacttat acaaaaaagt agtagctaat
    65821 gagctactac ttttgattta agagataaaa ccttttatcc atcacaatta atgttgctaa
    65881 atagatttta ttttatcatt aatatttgct tttaaacttt ttagctaaac taaataatac
    65941 actcagcata acgatagcca ttccagtgat ataataacca cgtttgctgt caccagtgga
    66001 tggcaatgta tttttaattt gtttttcaac atgtgtctga tgtgaagtta tctgtttatt
    66061 gtctgttcta gccatatgaa ctgcggaaac tgtcttagtc tttggtaaat cagacgcttt
    66121 aactgtagta tcagtttgat gaacgttatt aactttgtag ccttcttcaa ctataggtgc
    66181 ttcttgggtt ttatgtccct cttcagcttt acgtgcttct tcgactttat gtgcttcttc
    66241 agctttacgt acttcttcag ctttatgtgc ttcttcagct ttacgtgctt cttcagcttt
    66301 acgtgcttcg tcagctttat gtgcttcttc agctttacga atagcttcta agtcaactac
    66361 tttaatcgga acatcaactt tatcaacacc accatttaca taatgaatcg atactttagc
    66421 gtttgttaac attcctgatt catttgtgtt tggagcttcg tcgattgtta atgtataagc
    66481 tcctgccatt tctggaacaa ctaagtgctt catcaacata tccacagtta ctttagctcc
    66541 tttgtcagct gtcatttggc ttgctttaac atcaaaacgg tttctataga tatgaagggt
    66601 ctcattacga cgataatttg ataagtcaac agcagataac ttaataatat tatctccaaa
    66661 ttctagtggt actgtcatat ctttagtgat ttctacacca tttctaaaac tataaagacc
    66721 ggttttatca acagattcaa gtaatgaatc atcttgatat aattttagtt ttagagagtc
    66781 atctttggca acgatgtgga taagagcagt atcagaagtc aaatcacgtt tagcaactgc
    66841 tgtgttaagc tctggaactg attggtcaaa gaacatgcga cgaacaatac ttgctttctc
    66901 tcctgcttct gttaccactt caaccctaat ccaagtgtta aaacgactaa gtggtaattt
    66961 agcccggaac tcatattgtt taccggcata aagtactcta cgtccactca ttaatggttt
    67021 tgttcgatta gtcaagtctt taccttgaat aataacaggg gttcctagca tgtctccatt
    67081 ttcattcacc attttgactt caacatgctt gcttttatca ccatccttga tgtgaccgat
    67141 aataccagat gctgtgattt ccttaccatt tagagttgcc tcactagcag tttccaataa
    67201 ggcaggagtt aagagaaata ctgatgggta tttatgttca ctaacaggat catcttgtgg
    67261 tccagcaact gcaaactgtc tcatcatcat attttcagta agagcactcg ctcctttaac
    67321 agcattaaaa ttaagtttag tatctgcagt gagattaatt cgaaggacta gactgtcact
    67381 tgtttcgtaa tacgctgaat ctttttgcat agtaagtagc atatccctca tatgcttatt
    67441 tttagataat ctaggactta aaggaagttg ttgaactttc ttacttacac tacccgtctc
    67501 cataatcaaa cttttaacat ttaatgaatt ttctgtttta tgtaatacaa atgttgtcca
    67561 tcctttagtt aaaggtaact cactaccttt accaacttct tttccattca cagtaacctt
    67621 actataatgt gaccagtcaa tattagcaaa ggttactttt gctgtatttt cattggcttg
    67681 agttgaatga ctgagctgtt cacctttttt agtcatatct tctttctgat gactatatgg
    67741 atcttcttac cctttataaa caactttaag acttgcacca ctggttaatt taaatttaag
    67801 gttaattgca tctttataaa tatagtatcc atctatctga ctttcagtta aaccaaaact
    67861 agagtcaaat tttttcaatt catttggaac accattttgg ataaggtata tttttttaat
    67921 aattgaagct gggtcttgag attgtttgaa aaacatatct aattcaactg ttccagtttt
    67981 tgcaataagt tgtcccttaa attcagaatc ctttcgtcca agtcgaatag ttttaaactt
    68041 actcatgtcc atatcgttta tatccaaaga aacatctttt tgttcaacca attctggcat
    68101 ctttccatct ttgaaagtgg tcatgatttc tgaacttgag ttaatgttat taatagtaaa
    68161 ctctagacga cgatttgtcg ggttgtattt taattggtaa gttttgctga gattaatgtc
    68221 ttgttcatca tttttcaaga tattatcaac agttttaacc tcattcaaac gacgaacaga
    68281 taaactatca atatgtctga catcatttgg taattcaata ccaatcttaa cagatttatc
    68341 tttaagcgct atacgagaag tttcttgcaa tttatcgtta acataaatct taccaccctt
    68401 gtctttaata gcagcaaagc ttgaaaatac aatggcatgg taatctactg tttcaccagt
    68461 agctaattgc tcaaaagtag tattgctcaa atagtcaacc gctgtgatat tgacatattt
    68521 tgtatctgca aaataatctg ttgtgatagt aatttcttta ttgcgattat tattacgata
    68581 aattttaaga ctagtgtcat caaattgatt agctgacaaa gcatttctag gaatgactaa
    68641 tttgccatca gattcgactt gtaatatttt gccatttact ttgctagcta aaatagggtc
    68701 atcaacaaag ctaagttgaa gagacttagc atatttaatt gctcctgatt tatcttcaat
    68761 agcgtctttt ttactatcct ttggtgcttc atccggcggc gtgtttaaat ctgggaaacc
    68821 tggaggagtt gataaactat ctttgggttt gcttggctta gttcccggtg gggttgaata
    68881 atcaaaactt ctcattgaag ttggtgctgg taaatcaatt cctggtggac ggtctaattt
    68941 tggtttttca acttttttat cttctttttt aaatcctgga ggtgtgataa ggtctaaatt
    69001 attattgcca ccaccaacat acttaacaga ttgaagataa acgtctttca aaccaatgcc
    69061 agtatcctga actttgaaag taattttttt ccagcgatct tctaacttct ctacagtagt
    69121 cttatctttg ggacttaagt aaggtttatt gttgtccatt ttgatatagt attgataaat
    69181 ttggacacca tcgccaccga cacctttatt attcaactgt actttaattt gataaatgta
    69241 ttgtttatcc ccttttgcaa tttaatcttt aatcttacca tcccaaatag aatctggcat
    69301 catggcaaaa ctattacgat aatctaggcg ataaagtttt ctaacgtcgt gtgatctacc
    69361 aagtacacga acttctttat tggtatctgg atctataata gaatattgaa catactccat
    69421 attacgcaaa gctgcaatac gaactcctgc tttgtcatag aaaggactat taggagagaa
    69481 aaatactgta ttgttataga ttggaagact caacatgccg ttagttgtaa aggttgatga
    69541 ggtcttgttg gtttctttat taacatagaa ttgaacgcga cgttttttgt caccgttacc
    69601 aatttcagaa atgccttcaa aagcatcaat agctttttgc tctgtccatg aaccgtaaaa
    69661 acctaaaaat ggtaagtgta aatcggctac accttcagta tcttttaagt taagataacc
    69721 ttccaagaaa ttattgcgag ttaaagcctc tgcatcagaa taatccacat caaggtcaac
    69781 agctaatgtc ttacctgctt ctacagtaac tttccttgta actgcatcag ctttttttga
    69841 tactaggtgt tctgaatttt gcgtacgaaa tcctttttca tcaactgggt ccgcaatcgc
    69901 ttctgatgag atgatatacg ttttattagt ttttccaaag ttacgaagta gaatgcgtgc
    69961 tttaaatttt ttctctttta attcacgtaa ctccaactta ccatctgcat tattatcgtt
    70021 agtccctgtt acagttgcaa cgactgtcgt aacaagagct ttggcaagat tcattgctcc
    70081 gctaccttgt tggcgcacaa agtaaggtgt tttgccatct tttgattgtt tattgataat
    70141 tggctgtgca gtgttcatca agagtaattt aataaaatca gcagcattat caacaggaat
    70201 ttttttatct gtaatgtatt gttttaaaac tgcagctgcc ccagctacct gaggtgcagc
    70261 cattgatgtt cctgatttat cagcatattg attatcctcc acagttgaga aaatatgacc
    70321 acctggagct gtaatctcag gttttagacg taagtctggt gtaggtcccc aagaagaata
    70381 agaagataat tgtcctgcta aactattatc aatagcttga cgctctgtta aaatagtaat
    70441 aattttattg ctattatttt tgatattgtt atacgtgctt ctggtaatgc gagcaatatt
    70501 aaatttagct gcttctgatt caactaataa ttgactgcct aaatcatctt taaccttcgc
    70561 attattgtaa agcaagatag cagctggatt taacttagta atcgcctgta ctttttctaa
    70621 aaatccatta gatttattat cttctggaat ttcaagcatg gcgattttac cacttaaatc
    70681 aaaatgtttt gacaattccg aaggagcttc tgctcccaaa tctacagcca atccaataat
    70741 agtttttgaa gcgtttgggg caacatgaag attaatgact tctgtaactt ctgttccaga
    70801 ttttttatct ttaaatttca aaacatgagc atgcttttta aggttctcca tagaggcaac
    70861 agccaaggta ttatcatcga gggctgggtt ggcaatcaag gctgtatcat aattttctgc
    70921 tagcggctta acaccataag tattaccgtc agtgatattt ccttcattac ctgctgctac
    70981 tgcaatgacg attcctgcct tacacgctct tgcaataact tcaagttcag gatagtcttt
    71041 cccctcatgt acaaatccag caggtgttcc aagactcata ttaacaacat cagcttttag
    71101 taaaatagca tcgtctagag cttttagcca cacatctgtg aaggttgtgg gattttgttg
    71161 gtcatctgaa aataccttca tcgctaaaat ttgagcattt ggtgcaacac catacaattt
    71221 ttgattatca tcattagctc ctacgatacc tgttacatgc ataccatgca ttacaccgta
    71281 gctatccttg aggttatcat tatagtcata atagttataa ccataaggta ctttttcgga
    71341 atagaatttt cctttgagat tcttttcatg aatcagtttt tcaatactag ttttattata
    71401 cttagcacga tttggttctt tgatttttag tgcttggtgt ttgaaatcaa cccctgaatc
    71461 aataactgca acaacagttc cctcaccttt atactgggag ttgttccaaa cttctggcaa
    71521 accaataagt tgccctgagg aagatagtaa aggttttgtc tgaacatatt cttgtgagac
    71581 atagaccctt ctaacatcag caatcgtttt taatttttca atttcttgag cttgaatatc
    71641 aagtgcgaca ccattaataa tagcatcgta acgcttcact ttgcttgaat caaagtgaac
    71701 acttttttca atattcttaa gaactttatc ttgctcttgc ttgatgcttt gtaatttttt
    71761 ctcaacatca gcttgattta actgtgcaac tgtttttttc tgttctgtcg cataatctaa
    71821 gatagattcc ttattaaatt caacaattac tcttactttt tgaggctttg gcacagccgg
    71881 agtagctttc tcagcaaccg ccccctgtgg tttagtcaca gtaactgcct tagttgcttc
    71941 tgtactctta ttgcctttct ctgagttttc ttactcgtat ttaggcaact gctttggctg
    72001 attaacaact tctggtgtaa cagttacttt tggagctacc ttcttagtat cttgagctaa
    72061 gttaggaatc tcaactgttt tttcattaat tgttgggggt gtggttgtaa taggttggct
    72121 aacgtctgag ttaacactag cttttactgt ttctggcctt tctactacct tatctctagg
    72181 cattactcgg ctctcagatt gaacagtttc acttttgaca accttattct ccttagcttc
    72241 aacaggagtt gtagttgctt gtgtactgtg agtcttagag gtatttagag ctgttgtgtc
    72301 tgcttgaact ggctgaggta ctttcgttac agtggtaggt gaggttgttg cttcatcagc
    72361 gtgaatctct gtacttgctg tagctagact aacaactgct actgtaccca ataaagcacc
    72421 gcataggctg cggcacactt tacttttcct aaatccaaat ttgtccgtct ttttataaaa
    72481 tgtcatggct tcctcgtttc taaatttttg tctacatctt taatcatcat taagaaataa
    72541 tccatgctga atactcggca acggatcaac catatcatca ccattgtcat aaacaatatg
    72601 attatctagc cctaaattgg ttatattatc aacggtacta tttttcaaaa caacagttac
    72661 ataccgacat atgtctgaaa attctaaata atgaagacgt gaatccataa aaaagacttt
    72721 gataggctct ttttgaggag gaatttcgac aacaactttt ccatcatagt gatgaatata
    72781 tttcgtattc tctataattc ttaaaatagt attagatgaa tcattatgac caataggttt
    72841 ggctgaaagg aattcactca taaaagaacc tcacattact taaccggttt gttaaccagc
    72901 taacgaaatt ttagcactcc tctaagtact tgtcaatggc aaatgttaag ttaaaatcgt
    72961 tttcagcaat aagttctttt atcttttttt acattagaaa atatttcagt ttttattatt
    73021 tctaattatt atcacaaaaa aacacctcta ttttctgatt ttctcataaa atgtggaaat
    73081 gatcagttta tcatatcaac tctaaccata attgttaaag actacccaag gtattataaa
    73141 aagcccttga aatacttcaa agtgcttttt atagatgcat atgtaggaac aacatgttgc
    73201 gagttagtta tctggtaata attcttttaa gtaaaccgct tctactttaa aagtattatc
    73261 aaaagcataa ccataaatat agccatctat cattgtttga taggtaatat ctatagattt
    73321 cccacattta taggaaattt ctttttgtaa ccatgcctta taaaataata atttagaatc
    73381 catatcacta ctaactgatt ctcctaggta gttttggtag gtttgttctg atctatcttt
    73441 gtatttctca atatcaaccc cagttgaacc actgggagca taggttagaa tatgatagtc
    73501 accagaatta gagtaactaa ttttcccctt tggcaaacca attaaataac tactaacact
    73561 agaaaaagat tcctgacaaa tcttattggt aaaacctaac ttttttaagc attggtacac
    73621 aaaataactc cctgttaaca gtttatcttt gcttacctgt ctatttgctt gttgatatct
    73681 catcaaccga tcgccagata aattattctc caatgtagac tgcctatttt tataaatcat
    73741 aatatagtta actttgtctt tttctgataa aggattcaat ttctgcctcc gctttttgta
    73801 aagatgcctc tgcttcttct ttaattcctt ggcttagtct tatcatataa cttttataga
    73861 tatcatagtc tctttcaaga cagctaattg ctttacgtat ctctcttaca tcaggtttct
    73921 ttaccaaata agcgcacttt tctttactga acttcttaat attccaaaat tgttcgttaa
    73981 agaaagggat acctataaag ggaatacctt gtgcagccat cgcataaact gttccttgcc
    74041 caccgtgtat aatagcgaaa taaatctctt tatctatggt tttaaaatca ataaaatcaa
    74101 ctatctcaat ataacttcct ggaatgttta tagcctcacc acagcttgtc agtaaataat
    74161 gataatgcct tggtaatgtc tttaaaaaag attccaccca atctcgttta aatgaagtac
    74221 caaagctgag cagtacagtc ttattgtctt gattaatttg gctgcatttt gaaaaaccta
    74281 cattatctat caaaataggt cctatcgttt tataggacga atctttatca aattcataaa
    74341 acaaagagta atctgttacc aagtttagat caccagtaaa taattctaaa gcattattaa
    74401 aaggtttgcc accatatttt ttaatatagt tattccacgt tttaacattt gttgaataag
    74461 tagtgattac tcttttgata aaatcctttg tttgaggctt actagcttta cttatcttcc
    74521 cattaggcaa tattccttct gagttttcaa caatttcact gatatgcgtt gcccctgaaa
    74581 taatccaaat taatggtatg ctttctaaac gacaacttgt aacaatagat agacggtaac
    74641 ctgtcaatac agcatcaaat ttttcttggc gtaacaagtc tctttcaata gaacagatgc
    74701 gttcaatttc ttcttctgat aaaaaattaa agtctgttga aagagaaaag aaagcatcat
    74761 atcgcttact attataaaca gatttatctt ctacacagtg gtgatagcga taagcatcat
    74821 tattaaataa aaatggataa taattgtctc caaggatatg aatatcatgt ccttgttcat
    74881 ataaacaatt agccagtttt gataatcttt ggctctctcc taaattcatc atgaaaggag
    74941 caaataaaaa tttactcatt ttatcacctt ttttcaataa tagcaaaaga gataccaccg
    75001 aagatcgaat aagataggac taaataatag cccttttcta tactttcatt aacagtatag
    75061 tccagttctt caccagcacc attagatgaa aatccaaact gaccagaagc aaggtttggc
    75121 atattataat actcagacaa gttcgctaag aaatcataat ctgaactaac tgccttcttc
    75181 cgctcattcc aaacgaaacc tttgatatct tttatggtta gtcctaagtc tgataataaa
    75241 ttttgaagcg cagcatcaaa aatagtcatc acatctgtga atgttttatg gctatatttt
    75301 aattgtttac tacctaatat tataggagaa ttatccaatg cttgacgaga gaggacttgt
    75361 gctgaacaat aatcagaacc gacaaacatt tgactatcat agtttaattg ttgccaccac
    75421 ataaaactca tgtctgtcca ctgattagca gaaacaagaa tcacatagtc tagattatcg
    75481 ttacgcatca tttccttggc atattgtata ccatcaagcg ctccactatt tgtcgaaatg
    75541 acagataaag gacctgttat tttaaaaatg atagaaagca taccagctgc tgcattcatt
    75601 actgtaaacg ggaatcgtga agcagaaaca tgtgcatatc cttctgttgt gatttgcttt
    75661 tcaatacctt caacaacctc aactggtcca gaaagtgttg taaatacaat tcctactttt
    75721 gaagtatctt gtttttttag attaatattg ctttctatta gtgcttgagc tgttgttacg
    75781 gcaaccattt tggaaaaatc atccattttc ctaaattgtg ctgggttgac agttttgggt
    75841 ggtctagccc ctttaaagcg taatgcttca aagtcgttga aattactagc aactttttca
    75901 taggttatag aaagtgattc attcttagaa atggaagcaa cagatgataa gatagccatt
    75961 ttaagatttt ctctagcagg taatgtttct agaggtgaat ctaaagatga caataagaca
    76021 ccactattat ttccaccaaa agcaaacgaa aaatttaaag catttcttat tgggtattct
    76081 ctcttttgat gatagacaaa attttctgga aaaccttcta tcccaatctc atttttagtt
    76141 gctggtacag tctgttcctc tattgccgct aaacaattaa tcaattcgat aatacctgca
    76201 gcccctagag tatgacccgt ttgccccttg gtactgctga tcaatgtcgt tgtcgggaaa
    76261 aacttaccat acatattttt ttccatttta tcattagctt gagtacctgt accgtgaccg
    76321 ttaatatagt caatctcact gtagtcaata cctgcttgag tcactagctg ctttgcaatc
    76381 tgtgccgccc cttcacctgt tggcttaggt gctgttatat gataaccatc tgaagtaata
    76441 agaccaccga taatttttcc atatttagct aaggactgat ctttgacaag aacaacaaaa
    76501 ccagcgccct cacccaaatt gattcctttt ccagaagaat agggctgaca tgccatttct
    76561 gtattaatag ctcctagtga tgtgaagcct gctaaagaaa tatcacttaa ctcatcacag
    76621 ccaccacaaa tagctaaatc acaatcgcca tcttgaagta attgtgttcc taatattacg
    76681 gcattattac ttgcagaaca ggcggttgaa ataacatacg aagctcccac aatatcatga
    76741 taagccatca attcatcagc aatatggtaa acagatgctt tttctaataa actagcatct
    76801 acttgacgct ctccttcttc aaattgatac aaggcatttt gaccagcact ctttccccca
    76861 agtgaggtcc ctaaacacac agcaatatta tgataagctt ttaaattaac acctgaagaa
    76921 gcaagagcct cttcaaaagc ggtaaaagca aatttaaaat tacgtgtctc atctttgtat
    76981 tgctcaggaa cctctgggtc actagttatg cttcctgtat aagattctaa aatagagtcg
    77041 tgatttttat ataaatgttt agaaattcct tcttttaagt cgaagagatg ctgtttatgc
    77101 tcgctataat tctttcccaa agaagaaata attcctattc cactaacata tacgctcatt
    77161 ctttgttcca actttctggg atacaataag ggcttgattg tgttttcaaa atttggccat
    77221 cttctgttac aaaagtaact cctgaatgag catatgtttg gttaacaatc atcaaaccta
    77281 ggtaattttc cgaagagtct ttctcgtctg ctatccaaaa aatctttcca acaatttggt
    77341 catcaaatag cactggtgtt cctgaagcga gtttttcctt cgttgagaat ccaacacttt
    77401 taactgttgc tgatctgatg tgctctaaca agctatcgcg acctctaaat tcttcctttg
    77461 tgaaatctag attccaagaa taacctattt catataaaga atattcttct tgttgataaa
    77521 tatcagtaat gggttgtccc acttcaaacc ttaaatatct atcaagttca tccccatact
    77581 ctgctatacc ttcacagaca tccgaaacaa aagtagcaaa aatagaagat ggtaggaaaa
    77641 attgatagcc aaattctcca gaaaaaccaa atctagcaag ataacctttc tcacctttat
    77701 aagtcatctc tatcaaagta cgaaaattaa gtgttgaaat atcatattca taaaatgact
    77761 gagcaatttc tcccgaatat cttccttcaa tttgcagcat tttatactcg tcagaaatat
    77821 ctgttacggt atagtcaaaa ttgatgttgt ctaaataaga atccaaagct ttatgactag
    77881 ccaaccaata tttatcatca aatttgtaga aagtcacatc atcataaact gttccatctt
    77941 cattgagtaa aaaagtgtag ccacaagtat ctgtatctgc aaatacaata ttcttaggaa
    78001 tcacaaggtc taaggcttct tcacattctt caccagtaat ttcatataaa tacccctcat
    78061 aaactttata acctttatct gttctcaatg tattataaat agtattcatt tttcttcaca
    78121 ctttctatat tctgtaatgc tcacatttgt agcataaggt gagttccttt taaacatgca
    78181 ccttattttt aaatgattgt tctctttgta agaataaaat attccatttt tagcagaaat
    78241 tagtatatat ttatcatcgc tagctacata caatactaat tgttgactat tttccaaaac
    78301 ataggattta ttatcatatt tgacatgttc cccttggcgg atttggacaa actttcctct
    78361 aattcgataa tcaaaagaaa tagaatataa ttcactgtct cttggaattt cattcttagg
    78421 caaaacaatc tgattatagg ctaaaaccag tttgtgacta ggataatgct ttctgttagc
    78481 tatcattctc ttgatgccat aatccttctc ttcttctaat gttaggttag agatatgttt
    78541 taaaaggtca gttgctgacg cttggtagtt gctgtcccat attggctgtt tttttagatt
    78601 gattaacgcc tcctccgatg atgcttgtga aatcctaaaa tgataagtca gtggaaaaga
    78661 aaggctatct gtatcaataa gacataaggg tctaaattgc atttgattgc tggctttgcc
    78721 aggaggagaa taggaaatca ttttatcttc attcgggagg taataatgga attgtctatc
    78781 ttcaaactta tctgctcgaa gaaaatcatc atgctcaatc gggtaacttc ctggaacatt
    78841 cacaagataa ccttcactat ctctaatcaa ctgctcttgg cagtgtaact tgaaagtctg
    78901 tttcaataat tttgggaaca tctctttagc cttttgatta catttataaa cacaagtgat
    78961 ttcttcgtca cttgtaaatg tcatgatttt ctcaattaaa gcaccttgat agaaacattt
    79021 gattgtaatg tgattttctt cttcttttac aactaatttc ttgtccaaag gttggctaaa
    79081 aatcaaaaaa ctatcttcaa gtatctttct tttctgcttt tcaaaaataa ggctgccatt
    79141 tggagagata agtaaatcat aaccttgaaa agttctctta agacaaatag ttgcatctct
    79201 ataatgcttt tttcgacgtt ccagttgaac atcgatatta ccataaaggg tttccacagt
    79261 tgcttgaatg taataataat tggcaaaacg acttaaagtt aggctagggt gagccctcgt
    79321 taagtttgcc aagtgatatt ttccagtttt aacccgaacc ctctttactt gttccgataa
    79381 aaagtcaact tgtaaaacat aacggttatc taacttagca atttccattt gaaacaagct
    79441 catttttaaa taataaggaa gtgaagcact atcttctgct ttaaataata tttcttcatc
    79501 agagactttg gtcctaattt ctgtctcctc ttccaaggga ttttcaattt cctccataat
    79561 ttgaaaagtt gatatgtcgt atgttttacc ataataattc gaaatacgaa acgtattcaa
    79621 tatcttaggg aggtgactac gcaatgatcg acaatgcagt atgtcttcat atgtcttatc
    79681 atactctttg aagccattca attttgataa agctaaggag ggtttattgt agttactgat
    79741 ttcggtatag atattagaaa acttcagttt tgttatcttc tttaagatag ttttatatag
    79801 ataagtgatt gcctgtccac tacgactttt cgaatcaatt aaaagaaaac cactaaagct
    79861 actattccaa tctaaacaac cataggtaat aaatttaaag aaagctgatg ttccaatcaa
    79921 aacaccatga tttttcagta aaaataaatg tgtcacttct ttaactttta acgttaaatc
    79981 aaggtcatca acagttaaag gcctaccgtc tggaaattga taaacatgat tcctattaaa
    80041 taaatgaacc acttcagaat aatcttctaa ggaagcttca gaaatcttta atttattatc
    80101 atctttcatt ttttaacctt tttaataata aaatgaattg ccaataaaat aagaatgagg
    80161 gcaatgatat aaatcaacat aaagtgatta ctcagccttt ctccatcagc attacgcaaa
    80221 acaacaccat atgattcttt atagttagag acagtggctt cgttggcttt gtcaaatacc
    80281 ttcgcaatag aacccttcat taagacctgt ttcaaaagag aattgatttg agtcaatgga
    80341 aaccatgtca taacctgttg taatgctttt cctaccgagc caattgagag atatactcct
    80401 gaaataaaac caataaaagt ccctacaatt gtgctaagag tcgtaaaagc tgaactagta
    80461 tgaataaagg ctaaaatagg taaaatcatc gccgcagata aaacagtccc taaggaaacg
    80521 atccctaaaa tagttaaata atccatagct gaatactcta gcaaggaatt aaagccgtta
    80581 aaaataccga tagcaaagat gcaagagaac atcgtcatga caataccaaa taaaacggca
    80641 aatatagcat aggacaattc aattttgata tttgatatag gactgacctt aaaatcttca
    80701 tttaattttt tttctttgtc ggacaccata actccaaagg cacctaatgt actagtcatt
    80761 gaaataatag tcgtcaaacc tgcaatgagc caataattaa ccatgtgaat agtatcactg
    80821 gatactgtat tactcctcaa tgctgctttt atagcatcta attgaatttt tcccaagaaa
    80881 atctgataga ccaaaattaa gataataact gataaaaaag acatgaaaaa cgctaaccga
    80941 tctcgcgtgt aaactttacg atttctagaa attagttcaa acattgtttt tcctttcttt
    81001 ttctctaagc aagttgagat acgccgcctc taagttcgaa tattcaacat taaatgtctc
    81061 aataatagat ttatcttgat tgtctgagat aacctgcatc atttcctcaa cagaaatatc
    81121 cttaaatact atctcccttt ctgaaaggac ttgacattta ttaacaaaat cagctatcga
    81181 aagttggtct aatgactttt ctggtttcaa taccacattc aagtttgttg tggaatgttg
    81241 ctcaataaag cttttaatat ctcccgaata ataaatgttt ccttcaataa gaacatttaa
    81301 aacatcacaa gcagacattt cttcaagata atgagtaatt agtacaactg tcatctgcga
    81361 ttgttgattt agttgtgcga tagcatccca taaatcgcga cgagattgag ggtctaaacc
    81421 agtcgttggt tcatctaata ataatagaga aggttgtggc agcaaggcgc gtgcaatatc
    81481 aacttttcgt ttttgacctc ctgataaact gccatatttt tgttttttta ttgctgtgat
    81541 atctagatac gtttgaagat ctttaaggcg attacgtact tgtgatttag ataaaccgta
    81601 caaagcacct ctagaaatta gattttcctc aaccgttaaa ttatcatcta agcgattact
    81661 ttggaagaca ataccaatgt gacgataaaa atctttaata gatagaggtt ttccatccac
    81721 ctcccactta atatcgcctg acgtttgctt aaaattttga attaaaaggt taaataaagt
    81781 tgttttacca gctccattag gtccaagaag gccataaaat tttccctctt caaagatgag
    81841 attaatgtct ctcaatgctt caaaagaacc atattttttt ccaatatttt tgagtttaat
    81901 ttccattttc tgataccaac atccttcctg aggcaacctc ttttttattc tcatctctaa
    81961 taacgacttg aatttctaaa agatgcccca attttgattg agatttacat gtaatgttta
    82021 gctggtctcc tggagtaaca attttcttga atttgaaatt cttcacactg gctaaataac
    82081 ctactttttc tgctattttc tctgctgtta attcatcaag agaggggagg ctaccatcaa
    82141 gtgcatctaa aatataaagt aaagctgcag attgagctgc catctcaatc atcaaaacac
    82201 ccggaactat aggacgttgt ggaaaatgtc cttgaaagta aggttcgttg atagtaatat
    82261 tcttacgaca ttcaatgata ccttttttat aactctgcac tctatccacc attagaaacg
    82321 gatagcgatg aggtaacatt tttataattt cttcactact tagcttggtc atctatatcc
    82381 tcactatttt caatcacaaa atctgctagt ctatttactg atgataatat tgaaatatca
    82441 tcatcattta gttcaacacc aaacgttgtt gaaatcccta ttgacagttc tagggcatca
    82501 atagagtcta gctctaatcc acgaccaaat aatggttgat cattcataat cagatcttta
    82561 tcaatatcca acattaaaga gtcaatcaaa atacctttaa tttcatcaac tacttcttga
    82621 cgtgttttta ttattttacg ttcttcagcc cagttaatca ttaatcattc ctccatcaat
    82681 cacaatattt tttcctgtaa tatagtttgc cttctcactt gctaaaaagc ttacgagcca
    82741 agccacctct tcaggttgtc caaacctttt tagaggaata agatgctctt ttagctcttc
    82801 tttattttgc aattcatttg tcatatctgt ttcaatgaat cctggactta cacaattaat
    82861 cgtaatacca tctgatgcaa attctttcgc tagagtttta gtaatggaaa tgatagcgcc
    82921 tttcgttgct gaatagttcg cttgccctat ctgccctgct ataccactag ttgaagaaat
    82981 attgataacc tttccctgta tacgcttagc cttcatgatt tttaacacgg ctttactcat
    83041 gttaactagc cccataatat ttatgttaat gacatccatc catttttcct tagacatcat
    83101 caagaaaaag ccatctctaa caataccagc attattaact aaacaatcaa taccgccata
    83161 ttctctaaaa attttagtca ccaatttttg tactgctttt gcatctgaaa tattgcattt
    83221 ataaacgtct aattttggta actcctcttg taaagctctt gccttagcat cattgctatt
    83281 ataaatagca atgactttgt aaccttcttt gtataactct tttgtaatag cccttccaat
    83341 tcctctggta ccaccagtaa ctattgctac tttcataact gtttcctccc cctctttttc
    83401 attttttgca actgactaga aataaaccta ttaggagaaa tatcaataat ctccgcatcc
    83461 ttttctatga tagtttcctt aatcgcacgc cagttaaaga gattggtcca ctgatataaa
    83521 atttcatcta ccaattgatc acaatgaaaa atatagtcat agtcatcatt ttgcgaaata
    83581 tctaggcttt caatcaattg tttatactgc tctttagatt catttaaaaa atcagtatga
    83641 tatggtgctt ttacgcctaa ctgctttaat ttataaaaat aacctttgtt agtcatcacg
    83701 tcatcaaaga aagaacggtt gacttgatcc atagca
//
LOCUS       DATROD010000002        74506 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.002, whole genome shotgun sequence.
ACCESSION   DATROD010000002 DATROD010000000
VERSION     DATROD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 74506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74506
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.002"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            14..556
                     /locus_tag="VBC74_000081"
     CDS             14..556
                     /locus_tag="VBC74_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEO6941068.1"
                     /translation="MSIIIGLMAAMFIIRLAYLKLSIANEKALRKNGAKEYGVGVSKA
                     ITVLHIIIYFSSVTEAVLTKASFNFVSVIGLSLMIFSVFMLHTVTRLLGRIWTVKLMV
                     DKNHQFVDHWLFRVVKHPNYFLNIAPELLGVTLLCHAKYTALFVLPIYAFVIYLRIRE
                     ENLLLKTIIIPNGIKKSRVS"
     gene            869..1783
                     /gene="glyQ"
                     /locus_tag="VBC74_000082"
     CDS             869..1783
                     /gene="glyQ"
                     /locus_tag="VBC74_000082"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEO6941069.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLKSLEL
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLQTGPVTSE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFTQPEYEHSKYSFEISDQVMLLENFE
                     KFEREAKRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEETRIKLLAEED"
     gene            1783..2424
                     /locus_tag="VBC74_000083"
     CDS             1783..2424
                     /locus_tag="VBC74_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6941070.1"
                     /translation="MNTLIVNSHPDFSNPYSFTTILQEKFIELYNEHFPNHQLSILNL
                     YDCVLPEITKEVLLSIWSKQRKGLELTADEIVQAKISKDLLEQFKSHHRIVFVSPMHN
                     YNVTARAKTYIDNIFIAGETFKYTENGSVGLMTDDYRLLMLESAGSIYSKGQYSPYEF
                     PVHYLKAIFKDFMAFDDFCVVRAEGTDILDRQVVLDKMNQDLREAFEAFYSKE"
     gene            2428..4467
                     /locus_tag="VBC74_000084"
     CDS             2428..4467
                     /locus_tag="VBC74_000084"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEO6941071.1"
                     /translation="MTKDLLLELGLEELPAYVVTPSEKQLGQKMVKFLEDHRLSFETV
                     QTFSTPRRLAVRVKGLADQQTDLTEDFKGPSKKIALDAEGNFSKAAQGFVRGKGLSVD
                     DIEFREVKGEEYVYVTKHETGKSAIDVLASVTEVLTELTFPVNMHWANNSFEYIRPVH
                     TLVVLLDDQALELDFLDIHSGRISRGHRFLGSDTEISSASSYEDDLRQQFVIADAKER
                     QQMIVNQIHAIEEKKNISVEIDEDLLNEVLNLVEYPTAFLGSFDEKYLDVPEEVLVTS
                     MKNHQRYFVVRDRDGKLLPNFISVRNGNAEHIENVIKGNEKVLVARLEDGEFFWQEDQ
                     KLNIADLVEKLKQVTFHEKIGSLYEHMDRVKVISQHLADKAGLSDEEKLAVLRAASIY
                     KFDLLTGMVDEFDELQGIMGEKYALLAGEQPAVAAAIREHYMPTSADGELPETRIGAI
                     LALADKFDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILEAFGWDIPLDELVTNLYG
                     LSFASLDYANQKEVMAFISARIEKMIGSKVPKDIREAVLESDTYIVSLILEASQALVQ
                     KSKDAQYKVSVESLSRAFNLAEKVTHSVSVDSSLFENNQEKALYQAILSLELTEDMHD
                     NLDKLFALSPIINDFFDNTMVMTDDEKMKQNRLALLNSLVAKARTVAAFNLLNTK"
     gene            4479..4736
                     /locus_tag="VBC74_000085"
     CDS             4479..4736
                     /locus_tag="VBC74_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEO6941072.1"
                     /translation="MDPKKIARINELSKKKKTVGLTGEEKVEQAKLREEYIEGFRRSV
                     RHHVEGIKLVDDEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            4825..5088
                     /locus_tag="VBC74_000086"
     CDS             4825..5088
                     /locus_tag="VBC74_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEO6941073.1"
                     /translation="MIELLLALLLWNILVFATYAIDKRRAIRGAWRISEKTLLIESLL
                     LGGFGAFLGGEVFRHKTTKWYFRLVWYIGMIIDLAIIWLIWCY"
     gene            5203..6711
                     /gene="glpK"
                     /locus_tag="VBC74_000087"
     CDS             5203..6711
                     /gene="glpK"
                     /locus_tag="VBC74_000087"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEO6941074.1"
                     /translation="MSSEEKYVMAIDQGTTSSRAIIFNKKGEKIASSQKEFPQIFPQA
                     GWVEHNANQIWNSVQSVIAGAFIESSIKPGQIEAIGITNQRETTVVWDKKTGLPIYNA
                     IVWQSRQTAPIADQLKQEGHTNMIHEKTGLVIDAYFSATKVRWILDHVPGAQERAEKG
                     ELLFGTIDTWLVWKLTDGLVHVTDYSNAARTMLYNIKELKWDDEILELLNIPKAMLPE
                     VKSNSEVYGKTTPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMN
                     TGEEMQLSQNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLRMIETSSESEGL
                     AQSSTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRD
                     VIDTMQVDSGIDIQQLRVDGGAAMNNLLMQFQADILGIDIARAKNLETTALGAAFLAG
                     LSVGYWESMDELKELNATGQLFQATMNESRKEKLYKGWRKAVKATQVFAQED"
     gene            6724..8553
                     /gene="glpO"
                     /locus_tag="VBC74_000088"
     CDS             6724..8553
                     /gene="glpO"
                     /locus_tag="VBC74_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEO6941075.1"
                     /translation="MEFSRETRRLALQRMQDRTLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVTNTPAANKVLSAEDVLKREPDLQKEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGAYIASHVKAEDFLFDDNNQIIGVRARDLLT
                     DQVIDIKARLVINTTGPWSDTVRNFSNEGKQIHQLRPTKGVHLVVDRQKLNVSQPVYV
                     DTGLNDGRMIFVLPREDKTYFGTTDTDYHGDLEHPTVTKEDVDYLLNIVNKRFPEAEL
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKLSDESFEELIDSVKDYIAHKNHREDVE
                     KAISHVESSTSEKELDPSAVSRGSSFERDDNGLLTLAGGKITDYRKMAEGAMETIINI
                     LDKEYNRKFKLINSKTYPVSGGEINPSNVDSEIEAYAQLGTLSGLSIEDARYIANLYG
                     SNAPKLFALTRQITEAEGLSLVETLSLHYAMDYEMALSPTDFFLRRTNHMLFMRDNLD
                     SLIQPVIDEMAKHYQWSDQDKTFYEEELHETLKDNDLAALKDN"
     gene            8565..9263
                     /locus_tag="VBC74_000089"
     CDS             8565..9263
                     /locus_tag="VBC74_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO6941076.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKNHNSGWIVITFGWG
                     LAVAIAALVSGNISPAHLNPAVSLAFAIKGDLAWGTAILYMIAQIIGAMLGSLLVYLQ
                     FRPHYEAAENKADILGTFATGPALKDNFSNFLSEVLGTLVLVLTIFAIGKYNMPPGVG
                     TMSVGMLVVGIGLSLGGTTGYAINPARDFGPRLLHALLPMKNKGDSDWTYSWIPIVGP
                     MVGAILAALIFAMM"
     gene            9351..10617
                     /locus_tag="VBC74_000090"
                     /pseudo
     CDS             9351..10617
                     /locus_tag="VBC74_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137245.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            10608..12038
                     /locus_tag="VBC74_000091"
     CDS             10608..12038
                     /locus_tag="VBC74_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6941077.1"
                     /translation="MRIEALMEKERRVQYRLLSFLRGSPQAIALKLALLETGLSRATL
                     LKYINNLNSYFEQEKVNCRIVYYKDKLFLEEDYNLSNQEVLKALMKDSIKYTILISLF
                     NQRQFTIVGLSQELMVSEATLNRHLAHLNELLAEFDIAISQGKQIGDELQWRYFYYEL
                     FKQLWSYDKCQNMIKKLDLDSLILLIERLAQHTLTREAQQNLGLWFSICHHRLLAMEK
                     LSDNLKPIVEHYQCNAFYKRLDAALVLYMSRFALEYREGEVLATFAFLHSQNILPINT
                     MEYIMGFGGPIIDCVTETIIYFKKESILADETSDQVIYQLGQLYSHYYFFKGHILVEQ
                     PDLEQTYRLIDHNMRDKLHDISKKIIANVNRIRPLTEDGCSLLTLHLLELLIFSKNSQ
                     KMPFRIGLDMTGNAVEQSLLEYRIRQHFSGNNSIQVEPYDEGKGFDMVIYQSRSRPYK
                     AKLTYCLNKGASERELQEIDSLIYDN"
     gene            12163..14148
                     /gene="tkt"
                     /locus_tag="VBC74_000092"
     CDS             12163..14148
                     /gene="tkt"
                     /locus_tag="VBC74_000092"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO6941078.1"
                     /translation="MTFDTIDQLAVNTVRTLSIDAIQAANSGHPGLPMGAAPMAYVLW
                     NKFLNVNPKTSRNWTNRDRFVLSAGHGSALLYSLLHLAGYDLSIDDLKQFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDLVDHYTYTLHGDG
                     CLMEGVSQEAASLAGHLKLGKLVLLYDSNDISLDGPTSQSFTEDVKGRFESYGWQHIL
                     VKDGNDLEAIAAAIEAAKAETDKPTIIEVKTIIGFGAEKQGTSSVHGAPLGAEGITFA
                     KKAYGWEYPDFTVPAEVAARFASDLQARGAKAEEAWNDLFAKYEVEYPELAAEYKEAF
                     AGQAETVELKAHDLGSSVASRVSSQQAIQQLSTQLPNLWGGSADLSASNNTMVAAETD
                     FQASNYAGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNLNVIRPADGNETNAAWQRAVSET
                     DRPTMLVLTRQNLPVLEGTSELAQEGVNKGAYILSEAKGELDGIIIATGSEVKLALDT
                     QDKLESEGIHVRVVSMPAQNIFDEQEASYQEQVLPSAVTKRLAIEAGSSFGWGKYVGL
                     NGLTLTIDTWGASAPGNRIFEEYGFTVENAVSLYKEL"
     gene            14360..14665
                     /locus_tag="VBC74_000093"
     CDS             14360..14665
                     /locus_tag="VBC74_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO6941079.1"
                     /translation="MKKECRDFYRQIQHTYNDISVREDAVLSSILLSASNGLIKTSDV
                     PRVAYELTQQLENNEIEKSFESLATVKELKKSAKKLSERYEQNKVTTGLLSKVFGNH"
     gene            14685..15419
                     /locus_tag="VBC74_000094"
     CDS             14685..15419
                     /locus_tag="VBC74_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6941080.1"
                     /translation="MIKFEHVSKVYGEKEALSDLTLSIKDGEIFGLIGHNGAGKTTTI
                     SILTSIIDATYGQVYIDDLLLTEHRDQIKKKIGYVPDSPDIFLNLTAEEYWYFLAKIY
                     DVAPEDIEARITKLVDIFELEEQRYNPIESFSHGMRQKVIVIGALLPNPDIWILDEPL
                     TGLDPQASFDLKEMMKEHAKNGKTVIFSTHVLAVAEQLCDRIGILKQGKLIFVGSLGE
                     LKMQYPDKDLETIYLELAGRQANREG"
     gene            15423..17027
                     /locus_tag="VBC74_000095"
     CDS             15423..17027
                     /locus_tag="VBC74_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6941081.1"
                     /translation="MNWSRIWELVKINILYSNPQTLSALRKKQEKHPKKEFSAYKSMF
                     RNQLFQILLFSIIYVFLFVSLDFKEYPGYFTFYIGIFTLVSIIYSFIAMYSVFYESDD
                     VKQYAYLPIKSEELYVAKIFATFGMSVTFLMPILTLMIVAYWRIIGGPLAVLLAIINF
                     AILFLSVTVISLYINSLIGRAIIRSANRKLISTILISLATFGAIVPLLFVNMTSQKMV
                     QGKLQDIAPIPYVRGYYDIVTAPFSMESLLNYYLPLLIILFLIGAIYKWVMPRYYQEL
                     LYGQVKQRKVHRQIDFSKRESINKTLVKHHLSSLQNATLLTNTFLMPLLYLAMFIVPI
                     LNNGKEIGRFFNENYFGIAFLAGILIGSLCVMPASIVGVGISLEKSNFYFIKSLPISF
                     SYFLKHKFVTLITLQLAVPTFIYFLVGFFLLKLSILVLLSFILGLVFMGLIEGQFIYR
                     RDYKHLFLNWQEVTQLFNRGLGQWLLVGSLFGMMIIGSFLIGISIFWSMVWNTVAVNI
                     IILIIGLLILSICQYLLLKNFWKKLV"
     gene            complement(17235..18621)
                     /locus_tag="VBC74_000096"
                     /pseudo
     CDS             complement(17235..18621)
                     /locus_tag="VBC74_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643962.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            18762..19565
                     /gene="proB"
                     /locus_tag="VBC74_000097"
     CDS             18762..19565
                     /gene="proB"
                     /locus_tag="VBC74_000097"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEO6941082.1"
                     /translation="MKRHFETTRRIVIKVGTSSLVQTSGKINLSKIDHLAFVISSLMN
                     KGMEVILVSSGAMGFGLDILKMDKRPQEISQQQAVSSVGQVAMMSLYSQIFSHYQTHV
                     SQILLTRDVVVFPESLQNVTNSFESLLSMGILPIVNENDAVSVDEMDHKTKFGDNDRL
                     SAVVAKITKADLLIMLSDIDGLFDKNPNIYDDAVLRSHVSEITDDIIKSAGGAGSKFG
                     TGGMLSKIKSAQMVFDNNGQMILMNGANPRDILKVLDGHNIGTYFAQGK"
     gene            19575..20828
                     /locus_tag="VBC74_000098"
     CDS             19575..20828
                     /locus_tag="VBC74_000098"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEO6941083.1"
                     /translation="MTYIEILGQNAKKASQSVARLSTASKNEILRDLARNIVADTETI
                     LTENARDVAKAKDNGISEIMVDRLRLNKDRIQAIANGIYQVADLADPIGQVVSGYTNL
                     DGLKILKKRVPLGVIAMIFESRPNVSVDAFSLAFKTGNAIILRGGKDAIFSNTALVNC
                     MRQTLQDTGHNPDIVQLVEDTSHVVAEELMQATDYVDVLIPRGGAKLIQTVKEKSKIP
                     VIETGVGNVHIYIDEFADLDMAAKIVINAKTQRPSVCNAAEGLVVHQAIAKGFLSQLE
                     KMLKESNQSVEFRADEEALQLLENAVAASETDYATEFLDYIMSVKVVDSFEQAISWIN
                     KYSSHHSEAIITNNISRAEIFQDMVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKLH
                     ARGPMGLEALTSTKYYINGTGQVRE"
     gene            20911..21858
                     /gene="rsmH"
                     /locus_tag="VBC74_000099"
     CDS             20911..21858
                     /gene="rsmH"
                     /locus_tag="VBC74_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEO6941084.1"
                     /translation="MTNDFHHITVLLHETVDMLDIKPDGTYVDATLGGAGHSEYLLSQ
                     LGPDGHLYAFDQDQKAIDIAHIRLKKYVDTGQVTFIKDNFRNLSSNLKALGVSEINGI
                     CYDLGVSSPQLDERERGFSYKQDAPLDMRMNREQSLTAYDVVNTYSYHDLVRIFFKYG
                     EDKFSKQIARKIEQVRAEKPISTTTELAEIIKSSKSAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDLLAVDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLQPKMELVNRKPILPSQEELEANNRAHSAKLRVARRIR"
     gene            21873..22199
                     /gene="ftsL"
                     /locus_tag="VBC74_000100"
     CDS             21873..22199
                     /gene="ftsL"
                     /locus_tag="VBC74_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEO6941085.1"
                     /translation="MTNEKRTEAVTQTLQRHIKTFSRIEKAFYGAIVITAIIMAVGII
                     YLQSNSLQVKQEVNQLNSKINDKQTEFDNAKQEVNELSNRDRITKIAKDAGLTIQNDN
                     IYRKVD"
     gene            22203..24461
                     /locus_tag="VBC74_000101"
     CDS             22203..24461
                     /locus_tag="VBC74_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6941086.1"
                     /translation="MTFFKKLKKIFLDYVIHIRDRRSPQKNRERVGQNLMILTIFLFF
                     IFIINFVIIVGTDSKFGVNLSKEAKKVYQQSMTVQAKRGTIYDRNGNPIAEDATTYSL
                     YAIISKNYTTATGQKLYVQPSQYEKVASILENKLGMKKNLVLKQLNQKKLFQVSFGSS
                     GSGLSYTKMADIKKTMEKSDIKGIGFSTSPGRIYPNGIFASQFIGFTLPQDDGDGKKL
                     VGNTGLEAALNKVLSGTDGKVTYEKDRSGNVLLGTATTERRAVNGKDIYTTLSEPIQT
                     VLETQMDVFAEKTKGKFASATVVNAKTGEILATSQRPTYNPSTLKGYDKKNLGTYNTL
                     LYDNFFEPGSTMKVMTLASAIDSKHFNSTEVYNSAQYKIADAVIRDWDVNEGLSSGSY
                     MTFPQGFAHSSNVGMVTLEQKMGRDKWLNYLSKFKFGYPTRFGMLHESGGLFPSDNEV
                     TIAMSSFGQGIGVTQVQMLRAFTSISNDGVMLQPQFISSIYDPNTGTSRTARKEVVGK
                     PVSKEAASKTRDYMVTVGTDPYYGTLYAAGAPVIQVGNQSVAVKSGTAQIAQEGGGGY
                     LQGKNDTINSVVAMVPSENPDFIMYVTIQQPEKFSITFWKDVVNPVLEQATAMKETIL
                     KPVLNDSEHQTKYKLSKIVGENPGHVAEELRRNLVQPIILGNGSKVSKVSKRPGANLA
                     ENEQLLVLTNKLTELPDMYGWSKANVEQFAKWTGIKVTYKGSTSGKVRKQSIDVGKSI
                     NKIKKIKITIGD"
     gene            24463..25473
                     /locus_tag="VBC74_000102"
     CDS             24463..25473
                     /locus_tag="VBC74_000102"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEO6941087.1"
                     /translation="MFLSIMAGVIAFVLTVIAIPRFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLVVALLVSLIFSIILSKENSGNLGATFGILSVVLIYGIIGFLDDF
                     LKIFKQINEGLTPKQKMSLQLIAGLIFYFVHVLPSGTSAINIFGFNLEVGYLYAFFVL
                     FWVVGFSNAVNLTDGIDGLASISVVISLITYGIIAYNQTQFDILLIIVIMIGALLGFF
                     VFNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLFIGFVYVFETSSVMLQVAYFK
                     YTKKKTGVGKRIFRMTPFHHHLELGGVSGKGNKWSEWKVDAFLWAIGIFMSAITLAIL
                     YL"
     gene            25571..26914
                     /locus_tag="VBC74_000103"
     CDS             25571..26914
                     /locus_tag="VBC74_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6941088.1"
                     /translation="MSFKDFNFKPYIQRALDELKFVDPTDVQAKLIPVVRSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDESSDDVQVVITAPSRELATQIYQATKQIAEHSEQEIRV
                     VNYVGGTDKLRQIEKLKVSQPHIVIGTPGRIYDLVKSGDLVIHKAHTFVVDEADMTLD
                     MGFLDTVDKIAGSLPKDVQILVFSATIPQKLQPFLKKYLTNPVMEKIKTATVIADTID
                     NWLLSTKGRDKNAQILELSKLMQPYLAMIFVNTKERADELHSYLSSNGLKVAKIHGGI
                     APRERKRIMNQVKNLEFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLSGTAITLYQPSDDSDIRELEKLGINFIPKVIKNGEFQDTYDRDRRNNREKSYQK
                     LDTEMIGLVKKKKKKIKPGYKKKIQWKVEEKRRKERRASNRAKGRAERKAKKQSF"
     gene            27052..27864
                     /locus_tag="VBC74_000104"
     CDS             27052..27864
                     /locus_tag="VBC74_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6941089.1"
                     /translation="MFKKIILSIATIAATASLAVSVQASEKVELKVATDSDTAPFTYQ
                     KDGKFKGYDVDVVKAVFKGSKYKVTFKTVPFDTISTGIDAGKFDLSANDFSYNKERAE
                     KYLFSDPISRSNYAVVGKKGSHYKSLSDLSGKSTEVLSGVNYAQVLENWNKNHPNKKP
                     IKIKYVSGTTGVTSRLKNIESGKIDFILYDAISSDYIVKDQSLNLSVSPLKGKIGNNK
                     DGLEYLLLPKDKKGKTLQKFINKRIKVLKEDGTLARLSKQYFGGDYVSNIDK"
     gene            27959..28762
                     /locus_tag="VBC74_000105"
     CDS             27959..28762
                     /locus_tag="VBC74_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6941090.1"
                     /translation="MNQFILTGGWSWYNNLVSQVPAGKLFSWKAVLDAIPSILERLPI
                     TLLLTVAGALFGLILALIFAVVKINRVKILYPIQALFVSFLRGTPILVQLMLSYYGIP
                     LFLKFLNQKYGFDWNINAIPASVFAITAFAFNEAAYTSETIRAAILSVDQGEIEAARS
                     LGMTSAQVYRRVIIPNAAVVATPTLINTLIGLTKGTSLAFNAGIVEMFAQAQIMGGSD
                     YRYFERYISVALVYWAVSFLIEQLGNAIERKMAIKAPHHLTDEIPGGVR"
     gene            28762..29505
                     /locus_tag="VBC74_000106"
     CDS             28762..29505
                     /locus_tag="VBC74_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6941091.1"
                     /translation="MIKLRQLTKSFSGQKVLDKLDLDIEKGQVVALVGASGAGKSTFL
                     RSMNYLEEPDYGTIEIDDFKVDFKSISKDDILTLRRKLAMVFQQFNLFERRTALDNVK
                     EGLKIVKKMSDQEATRIARDELAKVGLADREKYYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKQGQTMVLVSHDMNFVYQVADKVLFLEKGRIL
                     ESGTPEQLFNHPLEERTKEFFASYNKSYL"
     gene            29627..29851
                     /locus_tag="VBC74_000107"
     CDS             29627..29851
                     /locus_tag="VBC74_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEO6941092.1"
                     /translation="MFLEIAKVYLQGLGLASITVILVGIFWVFWRAKKNIDKTARERQ
                     SVLYDVLLIALMTAPILAFAFVAIILMIKA"
     gene            29920..30834
                     /gene="trxB"
                     /locus_tag="VBC74_000108"
     CDS             29920..30834
                     /gene="trxB"
                     /locus_tag="VBC74_000108"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEO6941093.1"
                     /translation="MYDTLIIGSGPGGMTAALYAARSNLKVGLIEQGAPGGQMNNTAE
                     IENYPGYDHISGPELSMKMYEPLEKFEVEHIYGIVQRVENDGDVKRVITEDESYEAKT
                     VILATGAKNSLLGVPGEEEYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAVFLT
                     QFAKSVTIIHRRDQLRAQKVLQDRAFANEKIKFVWDSVVKEIKGNEIKVSGVTVENLK
                     TGEISEMTFGGVFIYVGLKPHSSMVSELGITDETGWVLTDTNMKTSIPGLYAIGDVRQ
                     KDLRQIATAVGEGAIAGQGVYNYITEHF"
     gene            30992..32452
                     /locus_tag="VBC74_000109"
     CDS             30992..32452
                     /locus_tag="VBC74_000109"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEO6941094.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFNKGIHNKKAVFEAYFRKVPFE
                     NGYAVFAGLERIVRYLENLSFSDSDLSYLEELGYPEEFLDYLKNLKMELTVKSAKEGD
                     LVFANEPLVQIEGPLAQCQLVETAILNIINYQTLVATKAARIRSVIEDEPLLEFGTRR
                     AQEMDAAIWGTRAAIIGGANATSNVRAGKIFNIPVSGTHAHALVQTYGDDYQAFKAYA
                     ETHKDCVFLVDTYDTLRVGVPNAIRVAKEMGEKINFLGVRLDSGDLAYLSKKVRQQLD
                     DAGFPNAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     TDAGSMRDTIKLSNNAEKVSTPGKKQVWRITSRAKGKSEGDYITFADTDVTQLDEIDM
                     FHPTYTYINKTVRDFDAVPLLVDIFDKGKLVYQLPSLQEIQEYGRKEFDQLWDEYKRV
                     LNPQDYPVDLARDVWQNKMDLIDRIRKEALAKGEVR"
     gene            32449..33270
                     /gene="nadE"
                     /locus_tag="VBC74_000110"
     CDS             32449..33270
                     /gene="nadE"
                     /locus_tag="VBC74_000110"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEO6941095.1"
                     /translation="MTLQDQIIKELGVKPVINPSQEIRRSVEFLKDYLLKHSFLKTYV
                     LGISGGQDSTLAGRLAQLAVEELRADTGENYQFIAIRLPYGIQADEEDAQKALDFIKP
                     DIALTINIKEAVDGQVRALNAAGVEITDFNKGNIKARQRMISQYAVAGQYAGAVIGTD
                     HAAENITGFFTKFGDGGADLLPLFRLNKSQGKQLLAELGADKDLYEKIPTADLEENKP
                     GIADEIALGVTYQEIDAYLEGKVVSDKSRGIIENWWYKGQHKRHLPITIFDDFWK"
     gene            33383..34717
                     /gene="pepC"
                     /locus_tag="VBC74_000111"
     CDS             33383..34717
                     /gene="pepC"
                     /locus_tag="VBC74_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014607929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEO6941096.1"
                     /translation="MSELTQTFTDKLLADYQANTKFSAIENAVTHNGLLKSLETRQSE
                     IENDYVFSIDLTKDEVSNQKQSGRCWMFAALNTFRHKLISDFKLENFELSQAHTFFWD
                     KYEKSNWFMEQIIATANQELSSRKVKFLLDVPQQDGGQWDMVVALFEKYGVVPKTVYP
                     ESVSSSASRELNQYLNKLLRQDAQILRELIAQGADGATVQNKKEELLQEIFNFLAMNL
                     GLPPQSFDFAYRDKDNHYQSDKNITPKAFYQKYVNLDISDYVSIINAPTVDKPYGQSY
                     TVEMLGNVVGGPAVKYLNLDMKRFKELAIAQMKSGETVWFGSDVGQVSNRQKGILATT
                     TYDFNSSMDIKLSQDKAGRLDYSESLMTHAMVLTGVDLDESGQPLKWKVENSWGEKVG
                     KDGYFVASDAWMDEYTYQIVVRKELLTKEELEAYNAEPITLAPWDPMGALAN"
     gene            complement(34764..37004)
                     /locus_tag="VBC74_000112"
     CDS             complement(34764..37004)
                     /locus_tag="VBC74_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6941097.1"
                     /translation="MITIKKESVIKLLKYAFGIIMGFIILAIVIGGLLFAYYVSRSPK
                     LTDQALKSVNSSLVYDGNNKLIADLGSEKRESVSADSIPLNLVNAITSIEDKRFFKHR
                     GVDIYRILGAAWHNLVSSNTQGGSTLDQQLIKLAYFSTNKSDQTLKRKSQEVWLALQM
                     ERKYTKEEILTFYINKVYMGNGNYGMRTTAKSYFGKDLKELSIAQLALLAGIPQAPTQ
                     YDPYKNPESAQTRRNTVLQQMYQDKNISKKEYDQAVATPVTDGLKELKQKSTYPKYMD
                     NYLKQVISEVKQKTGKDIFTAGLKVYTNINTDAQKQLYDIYNSDTYIAYPNNELQIAS
                     TIMDATNGKVIAQLGGRHQNENISFGTNQSVLTDRDWGSTMKPISAYAPAIDSGVYNS
                     TGQSLNDSVYYWPGTSTQLYDWDRQYMGWMSMQTAIQQSRNVPAVRALEAAGLDEAKS
                     FLEKLGIYYPEMNYSNAISSNNNSSDAKYGASSEKMAAAYSAFANGGTYYKPQYVNKI
                     EFSDGTNDTYAASGSRAMKETTAYMMTDMLKTVLTFGTGTKAAIPGVAQAGKTGTSNY
                     TEDELAKIEATTGIYNSAVGTMAPDENFVGYTSKYTMAIWTGYKNRLTPLYGSQLDIA
                     TEVYRAMMSYLTGGYSADWTMPEGLYRSGSYLYINGTTTTGTYSSSVYKNIYQNSGQS
                     SQSSSSTSSEKQKEDKNTADDANSSSPQVETPNNGNATTPNNSNQTVPGTGHGNGNNN
                     TAPNGN"
     gene            complement(36991..37590)
                     /gene="recU"
                     /locus_tag="VBC74_000113"
     CDS             complement(36991..37590)
                     /gene="recU"
                     /locus_tag="VBC74_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEO6941098.1"
                     /translation="MVNYPHQLIRKTTVTKSKKKKIDFANRGMSFEAAINATNDYYLS
                     HELAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYSGVYKGYYIDFEAKETR
                     QKTAMPMKNFHAHQIEHMANVLQQKGICFVLLHFSTLKETYLLPANELISFYQIDKGN
                     KSMPIDYIRKNGFFVKESAFPQVPYLDIIEEKLLGGDYN"
     gene            37665..38183
                     /locus_tag="VBC74_000114"
     CDS             37665..38183
                     /locus_tag="VBC74_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEO6941099.1"
                     /translation="MKSTILVTGYKNFELGIFQDKDPRITIIKKAIDKDFRRFLENGA
                     DWFIFMGNLGFEYWALEVALDLQKEYDFQIATIFTFENHGQNWNEANKAKLALFKQVD
                     FVKYTFPSYENPGQFKQYNHFLINNTQGAYLFYDSENETNLKFLLEMMEKKEAYDISF
                     LTFDRLNEIYEE"
     gene            38304..38636
                     /gene="gpsB"
                     /locus_tag="VBC74_000115"
     CDS             38304..38636
                     /gene="gpsB"
                     /locus_tag="VBC74_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEO6941100.1"
                     /translation="MASIIYSPKDIFEQDFKVSMRGYDKKEVDVFLDDVIKDYENYLE
                     QIEKLQMENRRLQQALDKKESEASNVRNSGTAMYNQKPIAQSATNFDILKRISRLEKE
                     VFGRQIRE"
     gene            38655..39023
                     /gene="rnpB"
                     /locus_tag="VBC74_000116"
     ncRNA           38655..39023
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VBC74_000116"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            39106..40260
                     /locus_tag="VBC74_000117"
     CDS             39106..40260
                     /locus_tag="VBC74_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEO6941101.1"
                     /translation="MKESFKLIATAAAGLEAIVGREIRNLGIDCQVENGRVRFHGDIK
                     TIIETNLWLRAADRIKIIVGEFPARTFEELFQGVYGLDWENYLPLGAKFPIAKAKCVK
                     SKLHNEPSVQAISKKAVAKKLQKVFHRPEGVPLQENGAEFKIEVSILKDKATVMIDTT
                     GSSLFKRGYRAEKGGAPIKENMAAAIIQLSNWFPDKPLIDPTCGSGTFCIEAAMIGMN
                     IAPGFNRDFAFEAWPWVDQSQVQKVRDEAESKANYDIDLDISGFDLDGRMVEIARKNA
                     EEAGLGDVIKLKQMRLQDLKTDKINGVIISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDEGFEKKYGSQADKKRKLYNGTLKVDLYQYYGERVRRQVK"
     gene            40273..41760
                     /locus_tag="VBC74_000118"
     CDS             40273..41760
                     /locus_tag="VBC74_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941102.1"
                     /translation="MSEDQKHPFFEPEKETDGLEFKDAKEMTVEEAVRKDSEIKAGIT
                     EEDSILDKYIKQHRDEVASQKFETKSSDFANLDTASLDDFIKKQREELSAMLAAEELS
                     KKLDNSVSQEQDIEANAVSPKEESSQEQENSVTPVPPLNTEAEPTATEPDSTIADSEE
                     YKSSSKKRGGIVGTLIALILLLIVTIFGYNYFKNNNSTNSQTATSQSSSSKATTTSSE
                     EDKKASQNLDNFNKAYANFFVDDKKTQLKNSEFDKLSELEKKVDALKGTKYYGKVKVK
                     FDSLKRQIDAVKAVNDKFKSPAVVDGKKSEKLEVKDGANFDSLDSKTLNTGNASLDSL
                     LHSIVSTGRNQVKQSEEQAKKTQEEQSSSNKVSDTQTTEQPNVTNGQSSSSAATINNQ
                     AAGTASGNLERNRSRVPYNNAAIADTGNPAWIFNPGVLEKIVATSQARGYFSGNNYIL
                     EPVNIINGNGYYNMFKLDGTYLFSINAKTGYFVGNAPGRADSLDY"
     gene            complement(41985..42467)
                     /locus_tag="VBC74_000119"
     CDS             complement(41985..42467)
                     /locus_tag="VBC74_000119"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002885585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEO6941103.1"
                     /translation="MTKEVVVESFELDHTIVKAPYVRLISEEVGPVGDIITNFDIRLI
                     QPNENAIDTAGLHTIEHLLAKLIRQQINGLIDCSPFGCRTGFHMIMWGKQDATEIAKV
                     IKSSLEAIAGGVTWEDVPGTTIESCGNYKDHSLHSAQEWAKLILSQGISDNAFERHIV
                     "
     gene            42656..44263
                     /locus_tag="VBC74_000120"
     CDS             42656..44263
                     /locus_tag="VBC74_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEO6941104.1"
                     /translation="MFNIILAMVCALIGLIIGYVAISMKMKSSKEAAELTLLNAEQDA
                     VDLRGKAEIEAEHIRKAAERESKAHQKELLLEAKEEARKYREEIEKEFKSDRQELKQM
                     EARLTDRASSLDRKDENLSNKEKMLDSKEQSLTDKSRHINEREQEIATLETKKVEELS
                     RIAELSQEEAKDIILADTEKDLAHDIATRIKEAEREVKDRSNKIAKDLLAQAMQRLAG
                     EYVTEQTITTVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVVLSGFDPI
                     RREIARMTLESLIQDGRIHPARIEELVEKNRLEMDQRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSYGQNVLRHSVEVGKLAGILAGELGENVDLARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPIVVNTIASHHGDVEPDSVIAVIVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIANGFEGVQNAFALQAGREIRIMVQPGKVSDDQVVIMSHKVRE
                     KIEQNLDYPGNIKVTVIREMRAVDFAK"
     gene            44392..45288
                     /locus_tag="VBC74_000121"
     CDS             44392..45288
                     /locus_tag="VBC74_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6941105.1"
                     /translation="MAKDIIKVDHIFKSIGQKTILEDISFSIASNQCVALIGPNGAGK
                     TTLMSTLLGDISISSGALTIFNLPAHHNRLKYKVAILPQENVLPSKFTVRELIDFQRC
                     LFPEVLPMSLILDYLQWSDAHLQQFTETLSGGQKRLLAFVLTLVGKPQLLFLDEPTSG
                     MDTSTRQRFWELVATLKKEGDTIVYSSHYLEEVEHTADRILVLHKGKLLRDTTPFAMK
                     QEKTEKLFTVPLSYQKLLPTYLITECEAKSDSITFVTGEAETVWKILADNGCPIEAIE
                     MTNRTLLNRIFETTKEVKHENL"
     gene            45275..46015
                     /locus_tag="VBC74_000122"
     CDS             45275..46015
                     /locus_tag="VBC74_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6941106.1"
                     /translation="MRIFKALLKMEFLLTKRQLANLIMAIGMPVAFFLFFSGFMGEGL
                     TKAIEAIYVRNYMITMAGFSSLSFAFFTFPFSMKDDQLSNRMQLLRHSPVPMWQYYLA
                     KIIRILFYYCLAITVVFLTGHILRQVSMPIEQWMQSFLLLLGGATCFIPFGLLVSYFK
                     NTELMSMVANICYMSLAVLGGMWMPITMFPKWLQGLSKLTPTYHLTQVILSPFANSFA
                     VFSLIILIGYGIIMLVIAYLLSQKRHSI"
     gene            46012..47097
                     /locus_tag="VBC74_000123"
     CDS             46012..47097
                     /locus_tag="VBC74_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6941107.1"
                     /translation="MKNKKNNYDLMYFIPLVFLIYPIGGILYYHYPFWTLFFTLAFVG
                     AYLYSIIIRGESKYHMIAWFTMLTYIFYMTVFINSGFIWYIYFLSNLLVYRFRDKLKS
                     FRFISFACTLATMVFLCFFKASDFGDRIMFLIVPIFCIGYMWIAIENRNSEEQREKIA
                     EQNQYINILSAENERNRIGRDLHDSLGHTFAMMTLKTELALKLLEKRNYDKVQKELSE
                     LNHISHQSMSEVRQIVSNLKYRTVVEEIDELYRLFQLSNIKLTVVNKLETSQLSPVTQ
                     STITMILKELSNNIVKHAEADSVELSLVRQGATINIEMIDNGCGFTNLDGDELHSIQE
                     RLTIVEGTLTILSRSKPTHIQVILKEV"
     gene            47099..47689
                     /locus_tag="VBC74_000124"
     CDS             47099..47689
                     /locus_tag="VBC74_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6941108.1"
                     /translation="MKLLVAEDQSMLRDAMCQLLLMEESVSTIDQAGNGEEAIAILSN
                     KAIDVAILDVEMPILSGLDVLEWVRKYQNVKVIIVTTFKRSGYFQRAIRSNVDAYVLK
                     DRSVADLMKTIQKVLSGGKEYSPELMENVISNPLSKQEIKILSLIAQGKTSKEIACTL
                     FLSNGTVRNYTSNIFDKLGVNNRLEALKIAREKDLL"
     gene            47739..48443
                     /locus_tag="VBC74_000125"
     CDS             47739..48443
                     /locus_tag="VBC74_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEO6941109.1"
                     /translation="MQKFKERFDTLSDAILAIAMTILVLEIKTPTTMGDIGDFTRNIG
                     LFIVSFVVVFNFWYERAQNSLDAQKTNDEIIALDIIEHLGICLIPLFTKFMISFENHN
                     FAVMAYGLLTLLVGLTSDIIRIRLASYDLVTIPSELKERVIKVMTTFAIRSVVVRFII
                     IILAYFLPEVGIFAYLVIPLFMFWRRSRAGKYYTKKGIEAPSYIKLFIQIGERVPEDF
                     TNSSSGISGVKRLYKK"
     gene            48610..49239
                     /gene="gmk"
                     /locus_tag="VBC74_000126"
     CDS             48610..49239
                     /gene="gmk"
                     /locus_tag="VBC74_000126"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEO6941110.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFDYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEALIKEGQMLEYAEYVGNYYGTPLSYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFIFLTPPDLEELEERLVGRGTDSPEVIAQRIERAKEEIALM
                     REYDYAVVNDQVSLAAERVKRVIEAEHYRVDRVIGRYTNMVKETDKKLS"
     gene            49262..49576
                     /locus_tag="VBC74_000127"
     CDS             49262..49576
                     /locus_tag="VBC74_000127"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEO6941111.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGEKATQDFKSV
                     KSTLRALEEIESGNVVIHPDPSAKRASVRARIEAERLAKEEEERKIKEQIAKEKEDGE
                     KI"
     gene            49650..52040
                     /locus_tag="VBC74_000128"
     CDS             49650..52040
                     /locus_tag="VBC74_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEO6941112.1"
                     /translation="MARKLAQVIVDIPLMQTDKPFSYAIPKDLEDLVQVGARVHVPFG
                     RGNRLLQGFVVGFRDDDELETKDIAGVLDFEPVLNQEQLDLADQMRHTVFSYKISILK
                     SMLPSLLNSQYDKLLLATDTLPSEDREDLFGHKTEIVFSSLSSQDAKKAGRLIQKGFI
                     EVQYLAKDKKTIKTEKIYKINRTLLEESQIAARAKKRLELKEFLLENPQPGRLTALNK
                     QFSSPVVNFFREEGIIEVIEKEASRSDNYFKGILKTDFLDLNQEQAKVVKIVVDRIGK
                     DQNKPFLLEGITGSGKTEVYLHVIDNVLKLGKTAIVLVPEISLTPQMTNRFISRFGKR
                     VAIMHSGLSDGEKFDEWRKIKSGQAKVVVGARSAIFAPLENIGAIIIDEEHESTYKQE
                     SNPRYHARDVALLRAEYHKAVLLMGSATPSIESRARASRDVYKFLELKHRANPKARIP
                     QVEIIDFRNFIGQQEVSNFTPYLLDKIRDRLDKKEQVVLMLNRRGYSSFIMCRNCGYV
                     DQCPNCDISLTLHMATKTMNCHYCGFEKPIPRTCPNCNSKSISYYGTGTQKAYEELLK
                     VIPDAKILRMDVDTTRQKGGHESILKRFGNHEADILLGTQMIAKGLDFPNVTLVGVLN
                     ADTSLNLPDFRSSERTFQLLTQVAGRAGRAEKEGEVVIQTYNPNHYAIQLAQKQDFEA
                     FYQYEMNIRRQLGYPPYYFTVGLTLSHKDEEWLIRKSYEVLSLLKQGFSDKVKLLGPT
                     PKPIARTHNLYHYQIIIKYRFEDNLELVLNRLLDITQDKENRDLRLAIDHEPQNMM"
     gene            52087..53022
                     /locus_tag="VBC74_000129"
     CDS             52087..53022
                     /locus_tag="VBC74_000129"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEO6941113.1"
                     /translation="MTKLLFMGTPDFSATVLKGILADGKYDVLAVVTQPDRAVGRKKE
                     IKMTPVKEVALENNIPVYQPEKLSGSPELEQLMTLGADGIVTAAFGQFLPTKLLESVG
                     FAINVHASLLPKYRGGAPIHYAIINGEKEAGVTIMEMVAKMDAGDMVSKASVEITDED
                     NVGTMFDRLAVVGRDLLLDTLPGYLSGDIKPIPQNEEEVSFSPNISPDEERIDWNKSS
                     RDIFNHVRGMYPWPVAHTLLEGNRFKLYEVTMSEGKGSPGQVIAKTKNSLTVATGDGA
                     IELKSVQPAGKPRMDIKDFLNGVGRNLEIGDKFGE"
     gene            53012..54334
                     /gene="rsmB"
                     /locus_tag="VBC74_000130"
     CDS             53012..54334
                     /gene="rsmB"
                     /locus_tag="VBC74_000130"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEO6941114.1"
                     /translation="MANDWKKSARGLALMTLEEVFDKGAYSNIALNKSLKKSRLSDKD
                     RALVTEIVYGTVARKITLEWYLSHFIVDRDKLELWVYHLLLLSLYQLLYLDNIPDHAI
                     VNDAVTIAKNRGNKKGAEKLINAVLRRVSSETLPEIASIKRQNKRYSVAYSMPVWLVK
                     KLIDQYGETRALAIMESLFERNKASLRVTDLSQKQTIKETLNVRDSHIAETALVADSG
                     NFASTSFFQDGLITIQDESSQLVAPTLKVSGNDQVLDACSAPGGKTSHIASYLTTGAV
                     TALDLYDHKLELVMENAKRLGLSDKIKTKKLDASKAHEYFPEDTFDKILVDAPCSGIG
                     LIRRKPDIKYNKANQDFEALQEIQLSILSSVCQTLRKGGIITYSTCTIFEEENFQVIE
                     KFLENHPNFEQVELSHTQEDIVKRGCISISPEQYHTDGFFIGQVKRIL"
     gene            54372..55109
                     /locus_tag="VBC74_000131"
     CDS             54372..55109
                     /locus_tag="VBC74_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEO6941115.1"
                     /translation="MEISLLTDIGQRRSNNQDFINQFENKAGVPLIILADGMGGHRAG
                     NIASEMTVTDLGSDWAETDFSELSEIRDWMLVSIETENRKIYELGQSDDYKGMGTTIE
                     AVAIVGDNIIFAHVGDSRIGIVRQGEYHLLTSDHSLVNELVKAGQLTEEEAASHPQKN
                     IITQSIGQANPVEPDLGVHLLEEGDYLVVNSDGLTNMLSNADIATVLTQEKTLDDKNQ
                     DLITLANHRGGLDNITVALVYVESEAV"
     gene            55109..57064
                     /gene="pknB"
                     /locus_tag="VBC74_000132"
     CDS             55109..57064
                     /gene="pknB"
                     /locus_tag="VBC74_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEO6941116.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLARDLILDNEEVAIKVL
                     RTNYQTDQIAVARFQREARAMAELTHPNIVAIRDIGEEDGQQFLVMEYVDGFDLKKYI
                     QDNAPLSNNEVVRIMNEVLSAMSLAHQKGIVHRDLKPQNILLTKKGTVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSILAENKSVPQALENIVIKATAKKLTDRYKTTYEMRRDLSTALSST
                     RHREPKLVFNDTESTKTLPKVTSTVSSLTTEQLLRNQKQAKTTEKITPDSASNDKTKS
                     KKKASHRLLGTIMKLFFALCVVGIIVFAYKILVSPTTIRVPDVSNKTVAQAKMTLENS
                     GLKVGAIRNIESDSVSEGLVVKTDPAAGRSRREGAKVNLYIATPNKSFTLGNYKEHNY
                     KDILKDLQGKGVKKSLIKVKRKINNDYTTGTILAQSLPEGTSFNPDGNKKLTLTVAVN
                     DPMIMPDVTGMTVGEVIETLTDLGLDADNLVFYQMQNGVYQAVVTPPSSSKIASQDPY
                     YGGEVGLRRGDKVKLYLLGSKTTNNSSSTPIDSSASSSTGTTTSDSVSSSTDASTSDS
                     SSTSTSSSTLPSDSTTNTGTANNPLTQ"
     gene            57223..57918
                     /locus_tag="VBC74_000133"
     CDS             57223..57918
                     /locus_tag="VBC74_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEO6941117.1"
                     /translation="MKKFQFFLLVEAVVLVMGLMKILSDDWTSFIFILALILLALRFY
                     NNDSRHNFLLTTSLLLLFLIFMLNPYIIAAVVFAVLYVLINHFSQVKKKNRYALIQFK
                     NHQLDVKTTRNQWLGTDQHESDFYAFEDINIIRISGTDTIDLTNVIVSGQDNVIIIQK
                     VFGDTKVLVPLDVAVKADISSVYGSVQYFDFEEYDLRNESIKLSQEEEYYLLKRVKLV
                     VNTIAGKVEVSRK"
     gene            57915..58934
                     /locus_tag="VBC74_000134"
     CDS             57915..58934
                     /locus_tag="VBC74_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6941118.1"
                     /translation="MKKHHYFLAFFYGSVIIFAICFVIIDSLGVNLVHLYQTSRLWLI
                     EQLIFSIFFLSLAVTILLLLTWFLLDDNSKRQINHNLRRILNNQSINVTDDGTEISTN
                     IQRLSKKMNLMTASLQSKENSRILKSQEIVKQERKRIARDLHDTVSQDLFAASMVLSG
                     IAQNVSQLDVDQVGSQLLAVEEMLQHAQNDLRILLLHLRPVELENKTLSEGFKMILKE
                     LTDKSDIEVVYHESILTLPKKIEDNIFRIGQEFISNTLKHSQASRLEVYLNQTENELQ
                     LKMIDNGIGFDMDSVYDLSYGLKNIEDRVEDLAGNLQLLSQPGKGVAMDIRLPLVNQS
                     EDKNG"
     gene            58927..59568
                     /locus_tag="VBC74_000135"
     CDS             58927..59568
                     /locus_tag="VBC74_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6941119.1"
                     /translation="MDKIKIVLVDDHEMVRLGLKSFLNLQADVEVIGEASNGLEGIKK
                     ALELRPDVVVMDLVMPEMDGVEATLALLKDWPEAAILVLTSYLDNEKIYPVIEAGAKG
                     YMLKTSSAAEILNAIRKVARGEQAIENEVDKKIKAHDKCPALHEGLTARERDILNLLA
                     KGYDNQRIADELFISLKTVKTHVSNILGKLNVADRTQAVVYAFQHHLVPQDDE"
     gene            59610..61010
                     /locus_tag="VBC74_000136"
     CDS             59610..61010
                     /locus_tag="VBC74_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEO6941120.1"
                     /translation="MDAKTKYKAKKIKAVFFDIDDTLRVKDTGYMPPSILKVFKALKD
                     KGIVVGIASGRARYGVPKEVQDLNADYCVKLNGAYVKDKDKNIIFHRPIPAEYVEQYK
                     KWADTVGIKYGLAGRHEAVLSDRDDLVNDAIDIVYSDLEVNPDFNKEHDIYQMWTFED
                     KGDSLHLPEPLDEHLRLIRWHDHSSDVVLKGTSKALGVSKVVEHLGLKPENILVFGDE
                     LNDLELFDYAGLAVAMGVSHPEAQKKADFITKKVEEDGILYALEELGLIEKELTFPQV
                     DIENTEGPVAVIKTNHGDMTVKLFPDHAPKTVANFIGLAKQGYYDGIIFHRIIPDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNVRGALSMANAGPNTNGSQFFIVQNTKIP
                     YAKKELERGGWPTPIAELYAGQGGTPHLDRRHSVFGQLVNQSSFEVLDEIAAVETGSQ
                     DKPLEDVVILTIEVKE"
     gene            61012..61368
                     /locus_tag="VBC74_000137"
     CDS             61012..61368
                     /locus_tag="VBC74_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEO6941121.1"
                     /translation="MKIGDKLKGTVTGIRPYGAFVSLEDGRTGLIHISEIKTGYIDNI
                     YDVLSVGDEVYVQVIDVDEFTQKASLSLRTLEEERHHIQHRHRFSNNRLKIGFKPLEE
                     NLPSWVEEGLAYLNNK"
     gene            complement(61414..62190)
                     /locus_tag="VBC74_000138"
     CDS             complement(61414..62190)
                     /locus_tag="VBC74_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEO6941122.1"
                     /translation="MTNEKGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQ
                     KMVPETMRDAITNESVIVGEEKSVDDIIEEVLKDIDFYEESGGGITLSGGEIFAQFEF
                     AKAILKRAKSLGIHTAIETTAYTRHEQFIDLIQYVDFIYTDLKHYNSLKHQEKTMVKN
                     ASIIKNIHYAFANGKTIVLRIPVIPNFNDSLEDAEEFACLFDRLDIRQVQLLPFHQFG
                     QNKYRLLNRQYEMEEIAALHPEDLLDYQAIFSKYNIHCYF"
     gene            62311..63066
                     /locus_tag="VBC74_000139"
     CDS             62311..63066
                     /locus_tag="VBC74_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO6941123.1"
                     /translation="MNRLENIISLVSQYQKIDVNTLSELLQVSKVTIRKDLDKLEGKG
                     LLHREHGYAVLNSGDDLNVRLSFNHKTKKEIAALAANMVSDNDTILIESGSTCALLAE
                     NICQTKRNVTILTNSCFIANYLREYDSCQIVLLGGEYQSSSQVTVGPLLKKMISLFHV
                     SLAFVGTDGFDPKLGFMGRDLMRSEVAQEMANAADEVIILTDSSKFNQTALVEQLPLS
                     TVSQVITDKHPNSDIANLFQEAEITIQSLLQVP"
     gene            63185..64168
                     /locus_tag="VBC74_000140"
     CDS             63185..64168
                     /locus_tag="VBC74_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEO6941124.1"
                     /translation="MKLERQKLLAKVAYLYYMEGKSQSEIANELGIYRTTISRMLAKA
                     REEGLVRIEISDFNPEIFQLESYFKSKYHLKDIEIVSSRKDSDTSEIEKDLAHVAAAM
                     IRKKIKENDKVGIAWGRTLSKVVEAMRPHPVSQVSFVPLAGGPSHINARYHVNTLVYE
                     MSRRFQGSCTFINATLVQENANLAKGILTSKYFEGLMDNWEKLDVAIVGVGGKPKSNE
                     QQWLDLLNQDDFQCLDEEAAVGEITCRFFNHSGDSVNQHLAKRTIGITLEQLQKVPNR
                     IAVAHGNYKAAALLAVLKKGYINHLVTDFSTALNILRLDKDTFVDTMYQKS"
     gene            64372..64695
                     /locus_tag="VBC74_000141"
     CDS             64372..64695
                     /locus_tag="VBC74_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEO6941125.1"
                     /translation="MEIIVADQIIMGLILNAGDAKQHIYQALKLAKEGNFAESKIEIE
                     LADSALLEAHNLQTQFLAQEAGGTRTDISALFIHSQDHLMTSITEINLIKEIIDLRQE
                     LHIQK"
     gene            64712..65032
                     /locus_tag="VBC74_000142"
     CDS             64712..65032
                     /locus_tag="VBC74_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6941126.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKIAADKEGIEAHIEAYSQGKIAD
                     YAKDLDVALLGPQVSYTLDKSKSICDEYGVPIAVIPMADYGMLDGVKVLKLALSLLET
                     RKEA"
     gene            65034..66335
                     /locus_tag="VBC74_000143"
     CDS             65034..66335
                     /locus_tag="VBC74_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6941127.1"
                     /translation="MSKFDSQKIITPIMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     LGQLPFKGLNQAIANVFGPEWTEPFMQVYSGTFAIMGLISCFAIAYAYAKNSGVEPLP
                     AGVLSLSSFFILMKSSYIPVKGEAIADAISKVWFGGQGIIGAIIIGLVVGAIYTWFIQ
                     HHIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYLIAKVTTGGTFIEMIYDIIQVP
                     LQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKSLLAANRLTL
                     DNGAHIVTQQFLDSFLILSGSGITFGLVIAMLFAAKSKQYKALGKVAAFPAIFNVNEP
                     IVFGFPIVMNPVMFLPFILVPVLAALIVYGAIAVGFMQPFSGVTLPWSTPAIISGFMV
                     GGWQGALVQIVILAISTAVYFPFFKIQDNITYKNECEMERG"
     gene            66516..68972
                     /locus_tag="VBC74_000144"
     CDS             66516..68972
                     /locus_tag="VBC74_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEO6941128.1"
                     /translation="MTKQLLTQKYTNDVQQNSQKHFGYLTERMYSYRDKVLDKKPFID
                     AERAILVTEAYQKHQEKPNVLKRAYMLQNILEKMTIYIDDETMIVGNQASSDKDAPIF
                     PEYTLEFVVNELDLFEKRDGDVFYITEETKEQIRNIAPFWENNNLRARAGVMLPEEVQ
                     VYMETGFFGMEGKMNSGDAHLAVNYQKLLEEGLIGFEKKARKAKADLDLTKPESIDKY
                     HFYDSILITIEAVKTYAERFAILAKKQAKTANTKRRQELLDIASICERVPYYPAETFA
                     EAVQSVWFIQCILQIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDSIVERLTNLWI
                     KTITINKVRSQAHTFSSAGSPLYQNVTIGGQTRHKEDAVNPLSFLVLKSVAQTHLPQP
                     NLTVRYHANLDKSFMNEAIEVMKLGFGMPAFNNDEIIIPSFIKKGVSEEDAYDYSAIG
                     CVETAVPGKWGYRCTGMSYINFPKVLLITMNNGIDPASGKRFAPSYGHFTQMTSYKEL
                     KEAWDKTLRYLTRMSVIVENAIDISLEREVPDILCSALTDDCIGRGKHLKEGGAVYDY
                     ISGLQVGIANLSDSLAALKKLVFEEKRLTTLEVWQALQSDYAGPRGEEIRQMLINEAP
                     KYGNDDDYADSLVRECYDVYVEEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGT
                     LATPDGRHAGTPLAEGCSPSHNMDKKGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTL
                     AKEEDKQKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAF
                     FNVLSKATQDDIIARTEHAL"
     gene            68982..69650
                     /locus_tag="VBC74_000145"
     CDS             68982..69650
                     /locus_tag="VBC74_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO6941129.1"
                     /translation="MEFLLDTLNLEAIKKWHHILPLAGVTSNPTIAKKEGDIHFFQRI
                     RDVREIIGCEASLHVQVVAKDYQGILDDAAKIRQETDDDIYIKVPVTPDGLAAIKTLK
                     AEGYNITATAIYTSMQGLLAISAGADYLAPYFNRMENLDIDATQVIKELAQAIERTGS
                     SSKILAASFKNASQVTKALSQGAQSITAGPDIFESVFAMPSIAKAVNDFADDWKASQH
                     SEHI"
     gene            69718..70806
                     /locus_tag="VBC74_000146"
     CDS             69718..70806
                     /locus_tag="VBC74_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEO6941130.1"
                     /translation="MKVFASPSRYIQGKDALFQSIEHIKSLGQTPLILCDDVVYNIVG
                     ERFLSYLQDDLLPHRVSFNGEASDNEINRVVAVAKEKNSDLIIGLGGGKTIDSAKAIA
                     DKVNLPVIIAPTVASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVIAQAPK
                     RLLASGIADGLATWVEARAVLQKNGIAMAGGRQTLAGVAIAQACERTLFNDSLQALAA
                     CDVKVVTKALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALSGDIHHLTHGEKVAY
                     GTLTQLFLENRPKEEIDRYINLYQAIGMPTTLAELHLGDATYEELLKVGQQATIEGET
                     IHEMPFKISAEDVAAALLAVDRYVSNHQ"
     gene            complement(70958..71884)
                     /gene="cysK"
                     /locus_tag="VBC74_000147"
     CDS             complement(70958..71884)
                     /gene="cysK"
                     /locus_tag="VBC74_000147"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEO6941131.1"
                     /translation="MSKIYNSITDLIGNTPIIQLHHIVPEGAAEVYVKLESFNPGSSV
                     KDRIALAMIEDAEQKGILKAGDTIVEPTSGNTGIGLAWVGKAKGYNVIIVMPETMSIE
                     RRKIIQAYGAQLVLTPGSEGMKGAIAKAKEISAEQNAWLPLQFNNQANPEIHEKTTGR
                     EIIETFGEKGLDAFIAGVGTGGTITGVSRALKKVNPDVAIYAVEADESAILSGEQPGP
                     HKIQGISAGFIPETLATDSYDHIIRVTSNDAIETGRIIGGLEGFLAGISASAAIYAAI
                     EVAKQLGKGKKVLALLPDNGERYLSTSLYDFE"
     gene            complement(71975..72619)
                     /locus_tag="VBC74_000148"
     CDS             complement(71975..72619)
                     /locus_tag="VBC74_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEO6941132.1"
                     /translation="MNYKTIKSDGIVEEEIKKSRFICHLKRVESEEEGRNYITQIKKA
                     HYKANHSCSAMVIGEKGDIKRSSDDGEPSGTAGIPMLTVLEKQGLTNVVAVVTRYFGG
                     IKLGTGGLIRAYSGSVANAIKEIGVVEVKEQIGIRIQLTYPQYQTFDNFLKEHHLQEF
                     ETEFLEAVTCKIYVDPKEFEHTITNLTEFYQGKALLTEEGSQIVEIPFKAETDK"
     gene            72675..73964
                     /locus_tag="VBC74_000149"
     CDS             72675..73964
                     /locus_tag="VBC74_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6941133.1"
                     /translation="MENYLGRLWTKAQLSEQLRKIAINLPSFIKKGSDYICTRCSSSV
                     AKNCQLPTGNYYCRECIVFGRVTSNENLYYFPQKTFSKTNSLKWKGGLTPYQNEVSEE
                     LLKGISSKENLLVHAVTGAGKTEMIYHSIAKVIDTGGSVCIASPRIDVCLELHKRLSN
                     DFRCAITLMHGESPSYQRSPLTIATTHQLLKFYHAFDLLIVDEVDAFPYVDNPILYHG
                     VKQALKKNGTSIFLTATSTTELERKVARKELKKLHLARRFHANPLVIPEMVWVSGIQK
                     SLQTQKLPPKLYQLINKQRQTRYPLLLFFPHIAEGQVFTEMLRQAFPMEKIGFVSSKS
                     TSRLKLVQDFRDNKLSILVSTTILERGVTFPSVDVFVIQANHHLFTKSSLVQISGRVG
                     RALERPEGLLYFLHDGKSKSMHQAIKEIKNMNHIGGF"
     gene            74253..>74506
                     /locus_tag="VBC74_000150"
     CDS             74253..>74506
                     /locus_tag="VBC74_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEO6941134.1"
                     /translation="MLKDVFTKEIKAALKRYKGYTIVPVPLSHEGYQNRQFNQVIAFL
                     QSANIPYKNILSKKDGGKQSANNKEERLKQVQQFTLKNEA"
BASE COUNT        24283 a        11833 c        15006 g        23384 t
ORIGIN      
        1 ataggagttt acaatgtcaa ttattattgg gttaatggcc gctatgttta tcattcgttt
       61 agcttattta aagctatcta tagctaatga aaaggcctta agaaaaaatg gtgctaaaga
      121 atatggtgta ggtgtttcaa aagcaatcac agttcttcat attatcattt atttttcatc
      181 tgtaactgag gctgttctaa caaaagcaag ttttaatttt gttagcgtaa tcgggcttag
      241 cttgatgatt ttttctgtgt ttatgttaca tacagtaaca cgtttacttg gtcgtatttg
      301 gacagtgaaa ttaatggtag ataaaaatca tcaatttgta gatcactggc tatttagggt
      361 tgttaagcat cctaactatt tcttaaatat tgctcctgaa cttttaggtg taactttatt
      421 gtgtcatgct aagtatacag cattatttgt cttgccaatt tatgcttttg ttatttattt
      481 aagaattcgt gaagaaaact tattattaaa aacgatcatt attccaaatg gaatcaaaaa
      541 gtctagggta tcttaagaat aagataaata gtagacataa aagtcatttt ttgttatact
      601 gtctcagtaa gaagaggtgt tcatcagctg tttttttagc gagctcaagg tagtgaaagt
      661 tgggtagaat aggatgaata ttggcacttt ttagtgagta aaagtacaat caaaaatgaa
      721 gtaataaatt agggtggaac cgcgtctttg acgcccctgt gctgttgggc ataggtggtg
      781 taggacgtgg tttttatcat ttgtccgttt taagtcaatt gttgtcacct tcccaacagc
      841 tgtaataaag tatgaaagaa ggaaaatgat gtctaagaaa ttaacttttc aagaaattat
      901 tctaacttta caacagtttt ggaatgacca aggctgtatg ttaatgcaag cttatgataa
      961 tgagaaagga gcagggacaa tgagccctta tactttctta cgtgctatag gccctgagcc
     1021 ttggaatgct gcatacgttg aaccatcacg tcgtccagct gatggccgct atggtgaaaa
     1081 tccaaaccgt ctttaccaac atcatcaatt tcaagtggta atgaagcctt cgccgtcaaa
     1141 tattcaagaa ctttatttga agtctttgga attactaggt atcaatccac ttgagcatga
     1201 tatccgtttt gttgaagata actgggaaaa tccatctaca ggttcagctg gattaggttg
     1261 ggaagtttgg cttgatggaa tggaaataac acaatttact tatttccaac aagtaggtgg
     1321 acttcaaaca ggtcctgtaa cttctgaagt tacttatgga ctagagcgtt tagcctctta
     1381 tattcaagag gtagattctg tttatgatat tgagtgggca ccaggtgtta aatatggtga
     1441 aatctttact cagccagagt atgagcattc aaaatattca tttgagatat cagaccaagt
     1501 aatgttactt gaaaactttg agaaatttga aagagaagca aaacgtgctt tagaagaagg
     1561 acttgttcat ccagcttatg actacgtcct taaatgttca cacaccttta atttattaga
     1621 tgcacgtgga gcagtatctg ttactgagcg tgcaggctat attgcacgta ttcggaattt
     1681 agcacgtgtt gttgctaaaa cctttgttgc cgaacgtaaa aaacttggtt tcccactttt
     1741 ggatgaggaa actcgtatca aattacttgc tgaggaagac taatgaacac actcattgtt
     1801 aatagtcatc ctgatttttc gaatccgtac tcatttacaa ctattctaca agaaaagttt
     1861 attgaacttt ataatgagca tttccctaac catcagttat ctattttaaa tttatatgat
     1921 tgtgttttgc cagaaattac gaaagaagta ctactttcga tttggtctaa acaaagaaag
     1981 ggtttggaat taacagccga tgagattgtt caagctaaaa tctctaaaga tttacttgaa
     2041 cagtttaagt cacatcaccg tattgtcttt gtttctccaa tgcacaatta caatgtgact
     2101 gctagagcaa aaacttatat tgataatatt tttattgcag gtgagacttt caagtacact
     2161 gaaaatggtt cggtcggatt aatgacagat gactatcgtc tgcttatgtt agaatctgcg
     2221 ggttctattt attctaaagg acaatatagt ccatatgaat ttcctgttca ttatttaaaa
     2281 gctatcttta aagattttat ggcttttgat gatttctgtg ttgttcgtgc agaaggtacc
     2341 gatattttag ataggcaagt tgttcttgat aagatgaatc aggatttaag agaagcattt
     2401 gaagcgtttt atagtaagga gtaggaaatg actaaagatt tattattgga gcttggactt
     2461 gaagagttgc cagcttatgt tgtgacaccg agtgagaagc aattgggcca aaaaatggtc
     2521 aagtttttag aggatcatcg tttatcattt gaaactgttc aaactttttc aactccacgt
     2581 cgtttggctg ttcgtgttaa aggattggca gaccagcaga cagatttaac agaagatttc
     2641 aagggtcctt ctaagaaaat tgctttggat gcagaaggga atttttctaa agcagcccaa
     2701 ggttttgttc gcggaaaagg tttaagtgtt gatgatatcg aatttcgtga agttaaggga
     2761 gaagaatatg tttatgtcac taagcatgaa actggaaaaa gtgcgattga tgttttagcg
     2821 agtgtgacag aggtattaac agaattgact ttcccagtga acatgcattg ggcaaataat
     2881 agttttgaat atattcgtcc agttcacact ttagtggtat tattagatga tcaagcctta
     2941 gagctagact tccttgatat tcattcaggg cgtattagtc gaggacaccg tttcttgggt
     3001 tctgacactg aaatttcatc tgccagttcg tatgaagatg acttgcgaca acaatttgtt
     3061 attgctgatg caaaagaacg tcagcaaatg attgttaatc aaattcatgc tatcgaagaa
     3121 aagaaaaata tttcagttga aattgacgaa gatttactaa atgaagtgct taatttagtt
     3181 gagtatccga cagcattctt gggttcattt gacgagaaat accttgatgt acctgaagaa
     3241 gttttagtca cttcaatgaa aaatcatcaa cgctattttg ttgttcgcga cagggatgga
     3301 aaattgttac caaactttat ttctgtgcgt aatggaaatg ctgagcatat cgaaaatgtt
     3361 ataaaaggca atgaaaaagt tttagtggct cgtcttgaag atggtgaatt cttctggcaa
     3421 gaagatcaga aacttaatat tgcagattta gttgagaaat taaaacaagt aactttccat
     3481 gaaaaaattg gttcacttta tgaacatatg gatcgtgtaa aagttatttc acaacatttg
     3541 gctgataagg caggtctttc tgatgaagaa aaattagctg tgcttcgagc tgcaagtatt
     3601 tataaatttg acctcttaac tggaatggtt gatgagtttg atgaattaca aggtattatg
     3661 ggtgagaaat atgccttact tgcaggggaa cagcccgcag tagcagcagc gatacgtgaa
     3721 cattatatgc cgacatcggc cgatggagag cttccagaaa ccagaattgg tgcaattcta
     3781 gcgttagctg ataaatttga caccttactt tcattcttct cagttggttt gattccaagt
     3841 ggttcaaatg atccatatgc tcttcgtcga gctactcagg ggattgtccg tattttggaa
     3901 gcttttggtt gggatatccc acttgatgaa ttagtaacta acctttatgg tctatcgttt
     3961 gctagtttag attatgctaa tcaaaaagag gtcatggcct ttatttcagc tcgtattgaa
     4021 aagatgattg gctctaaggt ccctaaagat attcgagaag ccgttttaga atcagacact
     4081 tatattgtta gccttattct agaagctagc caagcattgg tgcaaaagag caaggatgct
     4141 caatataaag tttccgttga aagtttgtca cgtgcattta atttagctga aaaagttact
     4201 cattctgtat cggtggactc ttcattgttt gaaaataatc aggagaaggc gctatatcaa
     4261 gctattttgt cacttgaact gactgaggat atgcatgaca acttagataa gcttttcgca
     4321 cttagtccaa ttattaatga tttctttgat aatacaatgg taatgacaga tgatgaaaaa
     4381 atgaaacaaa atcgtctggc tttactcaat agccttgttg caaaggcaag aacagtcgct
     4441 gcttttaact tattaaacac aaaataagga ggcattttat ggatcctaaa aaaattgctc
     4501 gtattaacga gctttcaaaa aagaaaaaaa cagttggatt gaccggagaa gagaaggttg
     4561 aacaagcaaa gctccgtgaa gagtatattg aaggtttccg ccgatcagtc cgtcaccacg
     4621 ttgaaggaat taaattagtg gatgatgagg gcaatgatgt taccccggaa aaacttcgtc
     4681 aagtccagag agaaaaagga ctacatggtc gtagtttgga tgatccaaat tcttaatggt
     4741 gagacaattt taaattaaag tcaacacttg ggtggggcaa aagataatct ttgtctcgcc
     4801 ttttttatta ggaaggaaaa aataatgatt gaattactat tagcattact gttatggaat
     4861 attttagtat ttgcaaccta tgctattgat aaaaggcgtg caattagagg ggcatggcgt
     4921 attagtgaga aaacattgtt gatagagagt ttattattag ggggatttgg tgcattccta
     4981 ggtggagaag tttttcgaca taaaacgact aaatggtatt ttagactagt ttggtatatc
     5041 ggaatgatta ttgatttagc tattatttgg ctaatttggt gttattgatg atatcttatc
     5101 aaaaatttta ggggcatatt aaatttttga tagtgcagga tagactgatt taggtataat
     5161 agaattacaa aaagaaaacg cttactttta aggaggaagt ttatgtcttc agaagaaaaa
     5221 tacgttatgg cgattgacca gggaacaaca agttctcgag caattatttt taataaaaaa
     5281 ggtgaaaaaa ttgcgagtag ccaaaaagaa tttccccaaa tttttcctca agcaggctgg
     5341 gtagagcata atgctaatca gatttggaat tctgttcaat ctgtcattgc tggagctttt
     5401 attgaatcaa gcatcaaacc tggacagata gaggctattg gtattaccaa tcaacgtgaa
     5461 acaactgttg tttgggataa aaaaacaggt cttccaattt ataatgctat tgtatggcaa
     5521 tcgcgacaaa cagccccgat tgctgatcaa ttgaagcaag aaggtcatac taatatgatt
     5581 catgaaaaaa ctggtcttgt cattgatgct tacttctcgg caacaaaagt tcgttggatt
     5641 ttagaccatg ttcctggagc acaggaacgt gctgaaaaag gagagttgct ttttggaaca
     5701 attgatactt ggttagtttg gaagttaact gacggtctcg tccatgttac agactattca
     5761 aatgctgctc gtacgatgct ttataatatt aaagaactta aatgggatga tgaaatttta
     5821 gagttactga atattcctaa agcaatgtta ccagaagtaa aatctaattc tgaagtttat
     5881 gggaaaacta ctccattcca tttttatggt ggagaggtgc caatttctgg tatggctggt
     5941 gaccaacagg cagcattatt tggacagttg gcctttgaac ctggtatggt aaaaaacact
     6001 tatggtacag gttccttcat tatcatgaat acgggcgaag agatgcaact gtctcaaaat
     6061 aatctattaa caacgattgg ttacggcatt aatggtaagg ttcattatgc acttgaagga
     6121 tcaatcttca ttgcaggaag cgctattcaa tggttacgtg atggtcttag aatgattgaa
     6181 acgtcaagtg agtcagaagg ccttgctcaa tcatcaacaa gtgacgatga ggtttatgtg
     6241 gttccagctt ttacaggttt gggtgcaccg tattgggatt caaatgctag agggtcagta
     6301 tttggattaa ctcgtgggac aagtaaagaa gattttgtta aagctacact tcagtcaatt
     6361 gcttatcaag tacgagacgt tattgacact atgcaagttg attcaggaat tgatatccaa
     6421 caacttcgtg tagatggtgg agctgcaatg aataatttat taatgcaatt ccaagcggat
     6481 attttaggga ttgatattgc tcgtgcgaag aaccttgaaa ctactgcctt gggtgcagct
     6541 ttcttagccg gactttcagt tggctattgg gaaagtatgg atgaactaaa agaactgaac
     6601 gcaacaggac aacttttcca agcaacaatg aatgagtctc gaaaagaaaa actgtataaa
     6661 gggtggagaa aagctgtcaa agcgacacaa gtatttgctc aagaggatta agggggtgta
     6721 gagatggaat tttcacgaga aacacgacgt ttagccttac aaagaatgca agatcgtact
     6781 ctggatttac ttatcatagg tgggggtatc acaggcgctg gtgtcgcttt acaagcagca
     6841 gctagtggat tagatactgg acttattgaa atgcaagatt ttgcagaagg gacaagtagt
     6901 cgctcaacaa aattggtcca tggtggtctt cgttacttga agcaatttga tgtagaagtt
     6961 gtttctgata cagtatcaga gcgtgcagtc gtgcagcaaa ttgcaccaca cattcctaag
     7021 ccagatccaa tgcttttacc ggtttacgat gagccgggtt ctacttttag tatgtttcgc
     7081 cttaaagtgg ctatggattt atatgattta ttagcaggtg tgacaaacac acccgcagcc
     7141 aataaagttt taagcgcaga agatgttctt aagcgtgagc ctgatttaca aaaagaaggt
     7201 ttgctaggcg gaggggtcta tttagatttc cgtaataacg atgcacgcct agtgattgaa
     7261 aatattaaac gtgctaatag ggatggagct tatattgcca gtcatgtcaa agctgaggac
     7321 ttcttatttg atgacaacaa tcaaattata ggagtacgtg cgcgtgattt gttgactgac
     7381 caagttatcg atataaaagc tagacttgtt attaatacaa ctggaccttg gagtgacacc
     7441 gttcgcaact tctccaatga aggtaaacaa attcatcaat tacgtccaac taagggagtt
     7501 cacttagttg tagatcgcca aaaattaaat gtttctcaac ctgtttatgt tgatacaggt
     7561 ctaaatgatg gtcgcatgat ttttgttttg ccacgtgaag ataagactta ttttggaacc
     7621 acagatactg attaccatgg agacttagaa catcctacgg taactaaaga agatgttgac
     7681 tacctcctta atattgtaaa caaacgcttc ccagaagcag agcttactat tgatgatatt
     7741 gagagttcat gggcaggtct gcgtccacta ctatcaggga atagtgcctc tgactataat
     7801 ggaggcaata gtggaaaact aagcgatgaa agttttgaag aattaattga ttccgttaaa
     7861 gattatattg cacataaaaa tcatcgtgag gatgttgaaa aagctatttc tcatgtagaa
     7921 tctagtactt ctgaaaaaga attggaccca tcagctgttt ctcgaggatc aagttttgag
     7981 cgtgatgata atggcctatt aacacttgct ggtggtaaaa ttactgatta tcgaaaaatg
     8041 gcagaaggtg ccatggaaac tattattaac attttggata aagaatataa tcgcaagttt
     8101 aaactaatca attctaaaac ctatcctgtt tcaggaggag agattaaccc atctaatgtt
     8161 gattctgaaa ttgaagctta tgctcaattg ggcacactaa gtggtttaag tattgaggat
     8221 gcacgttata ttgctaacct ttatgggtca aatgcaccaa aattatttgc attgacacgc
     8281 caaattacag aagcagaagg tctgagcttg gtagaaacgt tgtcacttca ctatgctatg
     8341 gattatgaaa tggcgcttag cccaacggat ttcttcttaa gacgtacgaa tcatatgctt
     8401 ttcatgagag ataatttaga ttctttgatt cagcctgtta ttgatgagat ggctaaacat
     8461 tatcaatggt ctgatcaaga caaaactttc tatgaagaag aattacatga gactctaaaa
     8521 gacaatgact tggcagcttt gaaggataat tgaaggagat aataatggat atttttggag
     8581 aattcttagg aacagctctc ttagtcttac ttggtaatgg tgttgtagca ggtgttgttt
     8641 tgcctaaaac gaagaatcat aattctggat ggattgttat cacatttgga tgggggcttg
     8701 ctgtagctat tgcagcgttg gtatctggca atattagccc tgcccacttg aatccagctg
     8761 tgtcattagc ttttgcgatc aaaggtgatt tagcttgggg aacagctatt ctttatatga
     8821 ttgcacagat tattggtgca atgctaggtt cacttcttgt ttatttgcaa tttagacccc
     8881 attatgaggc tgcagaaaat aaagcagata tccttggaac ttttgcaact ggtccggctt
     8941 tgaaggataa tttttccaat tttttaagtg aagtattggg aacacttgtt cttgtactta
     9001 ctatttttgc catcggaaaa tacaatatgc ctccaggagt tggtacgatg agtgttggta
     9061 tgttagtggt tgggattggt ctatcgctcg gtggaacaac aggttatgct attaatcctg
     9121 cacgtgattt tggtcctcgt ttattgcatg ccttgttacc gatgaagaac aaaggtgatt
     9181 ctgattggac ttattcatgg attccgatag taggacctat ggtaggagct attttagcag
     9241 ccttaatatt tgctatgatg taagagattt ttgatgctga gaccgacttt atggtctcag
     9301 ttttttgtta ccgttttcaa aaataacttc ttttgttata ataagtaatt atgacagaaa
     9361 aatatgtaaa taaagtaatt gtcattttag gagcttcttt tgctggtatg acttgtgcgc
     9421 agaaattacg ccaattaaat cctaattggg atatcgtttt gattgataaa gaaattcatc
     9481 cagattatgt tccaaatggc ttaaattggt attatcgtca cgaaattagt ggtttaaatc
     9541 aggccatgtg gcagacagaa gaagagcaac ggttgcagaa tatacgctgt ctattatgat
     9601 caattgattt gtgccatggg ctcccaagca gaatcaactt atattgatgg ggctgatgcg
     9661 caaggtgttt taacaacaaa aacttatgca actagtcaaa atgctaagca ggtattagat
     9721 aaaagtcata aggtagcagt tgttggggca ggtatcatcg gtttagatat tgcttatagt
     9781 ttacatgaat cagggaaggc ggtcactttg ttagaagctc aagaaagacc agacttcagg
     9841 catacagatc ctgatatgtc ccttccttta cttgatgcga tggctgaatc aaaactacat
     9901 tttttccaaa atcaaaaggt tgagaaaatt actgtaaccc gagaagaaaa actttgtttg
     9961 cgaacgctaa caggagacac ctttacagtt gatactgtca tattagctgt taattttcgt
    10021 ccggatagtc gtcttttaac tgggttggtt gatttaagtg ttgataatag tgttgttgta
    10081 aatgattatt tccaaacgag tgaccctaat atctatgcta ttggcgattt gatttggtcg
    10141 tattttaaag gtcttaatag tgcttattat atgcccttaa ttaaccaagc aatccgttcg
    10201 gcacaaatgt tggcatacca tttatcggga catgctgttc caaaattaaa aataacacgc
    10261 gctacaggat ctaaacactt tggctattat agagctaaca ttggattaac tgagctggaa
    10321 gctggtcttt acgaggatac cgttagtgtt acttatttcc ctaaagaaca gtatgattta
    10381 aggataaaat tgattgctaa tcaaaaaacg ggccatttat tgggggctca attaatctca
    10441 aaggaaaatt gtttggctac tgccaatcag cttgttcaag ctatatcgtg tgacatgaca
    10501 gattttgatt tggcttttca agatttcatt tatacagcta gagaatctga gatggcttat
    10561 atgttacatc aagcagctat caacctttat gaaaagagga ttgggctatg cgaatagaag
    10621 ctctaatgga aaaagaaagg cgtgttcaat atcggctact ttctttttta agaggctctc
    10681 ctcaggcaat agcactgaag ttagctttat tagaaactgg tttaagtcga gcaactttgc
    10741 ttaaatatat caataatctt aatagctact ttgaacaaga aaaagttaat tgtcgaatcg
    10801 tttattataa ggataagtta tttttagaag aggattataa cttatccaat caagaagtgt
    10861 tgaaagctct gatgaaagat agtattaaat acactatctt aatatcttta tttaatcagc
    10921 gtcagttcac aatcgttggt ctttctcaag aattgatggt tagtgaggct actttaaatc
    10981 gtcatcttgc tcatctcaat gaattgttag cggaatttga tattgctatt tcacaaggaa
    11041 aacagattgg tgatgaattg caatggcgtt atttttatta tgaattattc aagcaactat
    11101 ggtcttatga caagtgccaa aatatgataa agaaattaga cttagactca ttaattctat
    11161 taatagagcg cttggctcaa catactttaa ctcgagaagc tcagcaaaat ctggggcttt
    11221 ggtttagtat ttgtcatcat cgtctccttg ctatggaaaa actatctgat aatctcaagc
    11281 ccattgtgga acattatcaa tgtaatgctt tttataaacg gttagacgca gccttagttt
    11341 tgtatatgag tcgttttgct cttgagtata gggaaggaga agttctggca acttttgctt
    11401 ttctacattc acaaaatatt ttaccaatca atacaatgga atacatcatg ggatttggag
    11461 gcccgattat tgattgtgta accgaaacaa tcatctactt taaaaaggag agtattttag
    11521 ctgacgagac atcagatcag gttatttacc aacttggtca gttatatagc cattattatt
    11581 tctttaaagg tcatatttta gttgaacaac ctgatttaga acaaacgtat cgacttattg
    11641 atcataatat gagggataag ttgcatgata tttcaaagaa gattattgca aatgttaatc
    11701 gaattagacc tttaacagaa gatggttgtt ctttgctgac tttacatttg ttggaattgc
    11761 ttattttctc gaaaaatagt caaaaaatgc cctttaggat tggcctagat atgacaggta
    11821 atgctgtgga acagtcattg ttggaatatc gcattcgaca acatttctct ggtaataatt
    11881 ctattcaagt cgaaccttat gacgaaggaa agggatttga tatggttatt tatcagagtc
    11941 gctcaagacc ttataaagcc aaactaacct attgccttaa taaaggtgct tcagagcgag
    12001 aactacagga aatagattca cttatttatg acaattaatc ccattactta attgaatggt
    12061 tttaattcct ccagaaattg ttaaaacgtt tacgtatttt atgttataat atcaaagacg
    12121 aacaatttta aatttagttg ttaaaaacag aaaggtttta tcatgacatt tgacaccatt
    12181 gatcaattag cggttaatac agtccgcacg ctttctattg atgctatcca agcagcaaat
    12241 tctgggcacc caggtcttcc tatgggagct gcgcctatgg cttatgtgct ttggaataaa
    12301 ttcttaaatg taaacccaaa aacaagtcgc aattggacaa accgtgaccg ttttgtactt
    12361 tcagctgggc atggttcagc tcttctttat agcctacttc atttagctgg ctatgattta
    12421 tcaattgatg atttaaaaca attccgccaa tggggatcaa aaacacctgg acatccagaa
    12481 gttaaccata ctgatggtgt agaagcaaca actggacctt tagggcaagg tattgctaat
    12541 gctgttggta tggcaatggc tgaagctcat ttggctgcta aatttaataa accaggcttt
    12601 gatttagttg atcattacac ttatacactt catggtgatg gttgtttgat ggagggtgtt
    12661 agccaagaag ctgccagtct tgctggacac ttaaaacttg gtaaattggt ccttctttat
    12721 gattcaaatg atatctcact tgatggtcca acgtctcaat catttacaga agatgttaaa
    12781 gggcgttttg aatcatatgg ttggcaacat atcttagtta aagatggtaa tgatttagaa
    12841 gcgattgctg cagcaattga agctgctaaa gctgaaactg ataaaccaac cattattgaa
    12901 gttaaaacta ttattggatt tggcgcagag aaacagggaa catcttcagt tcatggagcg
    12961 ccacttggtg cagaaggtat tacttttgcc aagaaggctt atggatggga atatccagac
    13021 tttactgttc ctgctgaagt tgcagctcga tttgcctcag atcttcaagc gcgtggtgcg
    13081 aaggcagaag aggcatggaa tgatcttttt gctaaatatg aagttgagta tccagaatta
    13141 gcagctgaat acaaagaagc ttttgctggc caagctgaga cagttgaatt gaaagctcat
    13201 gacctaggtt catcagtggc tagtcgtgtt tcaagccaac aagctattca acaattatcg
    13261 actcaattac cgaacctctg gggagggtca gcagacctct cagcttctaa taacacaatg
    13321 gtagcagcag aaacagattt ccaagcaagt aactacgctg ggcgtaacat ttggtttggt
    13381 gtacgtgaat ttgcaatggc tgctgcaatg aatggaattg cgcttcatgg tgggactcgt
    13441 gtttatgggg gtacattctt tgtcttctca aactacttgt taccagcagt acgtatggct
    13501 gcacttcaaa acttgccaac tgtctatgtt atgacacatg attctattgc tgttggagaa
    13561 gatgggccaa ctcatgagcc aatagaacaa ttagcatcag tgcgctcaat gcctaatttg
    13621 aacgttatcc gtcccgcaga cggtaatgaa acaaacgctg catggcaacg agctgtatca
    13681 gagacagatc gaccaacgat gcttgtttta acacgtcaaa acttaccagt tcttgaagga
    13741 acatcagaat tagcccaaga aggggttaat aaaggtgctt atattttatc agaagctaaa
    13801 ggtgaacttg atggtatcat cattgcaaca ggttcagaag ttaaattagc tttagatact
    13861 caagacaaac ttgagtcaga gggcatccat gttcgcgtcg tatctatgcc agcacaaaac
    13921 atttttgatg aacaagaggc ttcatatcaa gagcaagtgc ttccatcagc tgttacaaaa
    13981 cgtcttgcta ttgaggcagg atcaagcttc ggttggggta aatatgttgg tttaaatggg
    14041 ctaacactta ctattgatac ttggggagca tcagcaccag gtaacagaat tttcgaagag
    14101 tatggcttta cagttgagaa tgctgtatcg ctatataaag aactttaaga gcatgaagaa
    14161 gttgggaaaa atacctcaac ttctttttgt aagatatagg atggatttga gaatagtttt
    14221 gatagactga taagtgatgg aaagcactct tttttgaaaa cgtttgacaa acattccata
    14281 gcgcagtata attgataaaa ctattgtata tataaaataa tacaactatt attaattatc
    14341 tttttagaaa ggtttgttta tgaagaagga gtgtcgtgat ttttatcggc agatacagca
    14401 tacttacaat gatatatctg tccgtgaaga tgcagtgcta tcatcaatac tattatcagc
    14461 atcgaatggt ttgataaaaa ctagcgatgt acctcgtgtt gcttatgagt taactcagca
    14521 attggaaaat aatgagatag aaaaatcttt cgagtcatta gctacagtta aggaattaaa
    14581 aaaatctgct aaaaaattgt ctgagagata tgagcaaaat aaggtaacaa cgggattgct
    14641 ttctaaagta tttggaaatc attaggagat aaaaggagat ttttatgatt aagtttgaac
    14701 atgtttcgaa agtatacggg gaaaaagaag ctctcagtga tttaaccctc tctattaaag
    14761 atggtgagat ttttggatta atcggtcaca atggagcagg aaaaacaacg acaattagta
    14821 ttctaacatc tattattgat gcaacttatg gccaagtcta tattgatgac ctacttttga
    14881 cagaacatcg tgaccaaatt aagaaaaaaa taggttatgt accggactcg ccagatattt
    14941 tcttgaattt aacagcagag gaatactggt actttttagc taaaatttat gatgttgccc
    15001 cagaagatat tgaagcacgt atcacaaaat tggtagacat atttgaacta gaggagcagc
    15061 gctataatcc tattgaaagt ttttcacatg gtatgcgtca aaaagtcatt gttataggtg
    15121 cccttcttcc aaatcctgac atttggattc tagatgagcc attaactggt ttagatcctc
    15181 aagcttcttt tgatttgaaa gaaatgatga aagaacatgc taaaaatgga aaaacagtta
    15241 ttttttcaac acatgtctta gcagttgcag aacagttatg cgacagaata ggtattttga
    15301 aacaagggaa attaatcttt gtcggtagct taggtgagtt aaaaatgcaa tatccagata
    15361 aagatttgga aactatctat ttggaattag caggacgtca agctaatagg gagggataaa
    15421 gtatgaattg gtcacgtatc tgggaactcg taaaaattaa tatcctttat tcaaaccctc
    15481 agactctatc ggcactaaga aaaaagcaag aaaagcatcc taaaaaagaa ttttcagctt
    15541 ataaatccat gtttagaaat cagttatttc agattttgct cttttcaata atttatgtat
    15601 ttctctttgt atcacttgat tttaaagaat atccgggcta tttcacgttc tacattggta
    15661 tctttacact agtatccatt atctactctt ttattgcgat gtacagtgtt ttctatgaga
    15721 gtgacgatgt taaacaatat gcttatttgc ctattaaatc tgaagagctt tatgttgcta
    15781 aaatttttgc aacatttggg atgtctgtaa cttttctaat gccaatccta accttaatga
    15841 ttgtggctta ttggcgtata ataggtggcc cacttgcggt cctcctagca atcataaatt
    15901 ttgctatttt atttttatcg gtaacggtga tttcattgta tattaatagt ttaataggcc
    15961 gtgccattat tcgaagtgct aatcggaaac ttatttctac tatattaatt tcactagcta
    16021 catttggagc gatagttccc ttattatttg tgaatatgac cagtcaaaag atggtacaag
    16081 ggaaattaca agatatcgct ccaattcctt atgtcagagg ttattatgat attgttacag
    16141 cgccgtttag tatggagagt cttctcaact attatctccc gctcttaatc attttattct
    16201 tgataggtgc tatttataaa tgggttatgc cacgctacta tcaggagttg ttgtatggac
    16261 aggtcaagca gagaaaagtc catcgtcaaa tcgattttag taaacgtgag tcaattaata
    16321 aaactcttgt taaacaccat ttatcaagtt tacaaaatgc gacattattg acaaatacat
    16381 ttttaatgcc actattatat ctagcaatgt tcattgttcc aattttgaat aatggtaaag
    16441 agattggtcg attttttaat gaaaattatt ttgggatagc ttttctagca ggaatattaa
    16501 taggtagttt atgtgtgatg ccagctagca ttgtaggggt aggtatttct ttagaaaaaa
    16561 gtaatttcta ctttataaaa agtttgccta tatcattttc gtatttctta aagcataaat
    16621 ttgtaacttt gataacatta caacttgcag ttcctacttt tatttatttc ttggtaggat
    16681 tctttctact taaactatca atcctcgttt tattgagctt cattttaggt ctagtcttca
    16741 tgggacttat cgaaggccag tttatttata gacgtgacta taaacatttg ttcttgaatt
    16801 ggcaagaggt tacgcaacta tttaatagag ggttaggcca atggctgtta gttggttcat
    16861 tgtttggaat gatgataata ggtagttttc ttattggaat aagtattttt tggtcaatgg
    16921 tatggaatac tgtagctgtc aatatcataa tccttatcat tgggttgctc atattatcaa
    16981 tttgccaata cttactgcta aaaaattttt ggaagaagct tgtttagtaa aaagatttta
    17041 gaaaaatcat acagtaagaa taatgcttaa taactgagat agttatagcg gataaaataa
    17101 aggcttgttg tggactttac ggttggaagg cagccactgt aatcctttgt ttaataacgc
    17161 tagttttagc tctaaaatat ttgattttta ttgctatcta ttatatctta tcagacaaag
    17221 agactaggga taacctagtc tctttgtctg atgctaactt gtttttcgta atattgtgct
    17281 agaatgaatg tcaatatagc agttgtgctt acagtaatga taactgcaat aagtatggta
    17341 ataaaaagat gtgttgaagt atgactgata taaagaaata gacctggtag accagaagag
    17401 cctacagcga agcggtgagc gttcattata ccggcataga gtcctccaag accaccacca
    17461 ataagtgagg ctattagagg ataacgtttc ggaaggttga caccatataa gataggttcg
    17521 gtgattccca tataagctgt tatggcagca ggtccagcaa tttctttaag ctttttttct
    17581 tttgttataa gagtcaccac tgtacctgca gttgcttgtg cgatatttga gcagagcatg
    17641 cctggtccaa aaatagaatc aacccctaat ttcgctagtt gtgtgattcc taatgcagca
    17701 attccactat gaacaccaaa catgattagg ataggagcaa atgctcctac tagaaaagca
    17761 ggtgcccaac tggcgtattt ggataaaaaa agtaaaaata acagctaaat attggccagc
    17821 gatagtaccc attggtccca atatagagaa cgacaaaatt cccatgctga gaaatattaa
    17881 cataggaaca agcactaaac gtagtgattt aggaataatt tgatttaaat atttttctat
    17941 ataagcctga acacaaataa ttagaataat gggaatgaca cttgatgcat agttagtaag
    18001 tgtgaatggg atcgtatgga ataaggatac tggtttacca gaagctacta aattagccca
    18061 attagggtga aggagcatga caacagttcc caaagctaaa ataggattag ccttcaattt
    18121 ttgagcagct gtgatggcga ttaagatagg taaaaaatag aaaacaccat ctgcaaaaag
    18181 atttagtaat atatatgttt gagataacgg tgtcaagatt ttgaatacta gtaataaagc
    18241 tagcaatgcc ttaatcatac cagcgcctga tagagctggt aaaatcggtt ggaaagtccc
    18301 agaaacaaaa tcaataattc gactaactgc tttggctttt ttggtttgtt ctgttttagt
    18361 cgttgagtta gtttcaatga gggagttaat ttcttcaaac acttttgcga catgagtccc
    18421 gatcactatc tgatgttgcc ctccgctgat aacgacagtt tttacctctt ttaaagtttt
    18481 taattgctca aggtttactt tcgtcggatc attgagacta aagcgaaggc gtgtttgaca
    18541 atggtaaaca ttgttaatat tattttgccc accaaggtgt ttgatgatat tttttgacaa
    18601 ggtgatgtat tctctagaca taatatctcc ttaatactta taaaacgttt tccgattcta
    18661 gattatagca tagtaataaa agaaaacact tggaaatgct tgtgaatttt caatgatgtt
    18721 ttcagatagc attcctaaag ggtctatgat ataatttttt tatgaaaaga cattttgaga
    18781 caactagaag aattgttatt aaagtaggaa ccagttccct cgtacagaca agtgggaaaa
    18841 ttaatttaag taaaatagac catttagcat ttgttatttc tagtcttatg aataaaggta
    18901 tggaggtgat tttagtatca tctggagcta tggggtttgg tttagacatt ttgaaaatgg
    18961 ataagcgtcc tcaagaaata tcacaacaac aagcagtttc tagcgtagga caagtagcca
    19021 tgatgagtct ttattctcag atattttcgc actatcaaac tcatgtttcc caaattttat
    19081 taacacgtga cgttgtggtt tttccagaga gtttgcaaaa tgtaacaaac tcttttgaga
    19141 gtctgctctc tatgggaatt ttgccaatcg tcaatgagaa tgatgctgtc agcgtggatg
    19201 agatggatca taaaacgaag tttggggaca atgaccgtct ctctgctgtc gttgctaaaa
    19261 ttactaaagc agatttatta attatgctgt cagatattga tggtcttttt gataaaaatc
    19321 ctaatattta tgatgatgct gttttacgta gtcatgtttc tgagatcacc gatgacatca
    19381 ttaagtcagc tggaggagca ggaagtaagt ttggtactgg tggcatgctt agtaaaatta
    19441 aaagtgcaca aatggtgttt gataacaatg gacaaatgat attgatgaat ggcgccaatc
    19501 ctcgtgatat tttaaaggtg ttagatgggc ataatattgg aacttatttt gctcaaggaa
    19561 agtgagattg agttatgacg tatattgaaa tattagggca aaatgctaaa aaagctagtc
    19621 aatcagtagc acgattaagt acagctagta aaaatgagat actcagggac ttagcgagaa
    19681 acatagtggc tgatactgaa accattttga ctgagaatgc tcgcgatgtt gctaaggcaa
    19741 aagataatgg tatctcagaa attatggtgg accgtctacg actaaacaaa gaccgcatac
    19801 aggcgatagc aaacggtatt tatcaggtag cagatttggc agatccgatt ggtcaagttg
    19861 ttagtggtta tacaaattta gatggtttaa aaatccttaa aaaaagagtg cctttagggg
    19921 ttattgcaat gatatttgaa agtcgtccca atgtatcggt ggatgccttt agtttagctt
    19981 ttaaaacagg gaacgctatt attttacgtg gtggtaaaga tgctattttt tcaaatactg
    20041 ccttggttaa ttgtatgcgc caaacattgc aagatacagg ccacaaccca gatatagtac
    20101 aattagttga agatactagt catgttgtag cagaggagtt aatgcaggca acagactacg
    20161 tggacgtctt aattccaaga ggtggtgcaa aattaattca aacggtgaag gaaaagtcaa
    20221 agattcctgt tatcgaaaca ggtgttggaa atgttcatat ctatattgat gaatttgcag
    20281 atttagatat ggcagctaag attgttatta atgctaaaac ccaacgccct agtgtctgta
    20341 atgctgctga agggttagtt gttcatcaag ctattgctaa gggattttta tctcaattgg
    20401 agaaaatgct caaagaatcg aaccagtcag ttgaatttcg tgccgatgag gaagctttac
    20461 aacttttaga aaatgctgtt gctgcaagtg agactgatta tgctactgaa tttttagact
    20521 atattatgtc agtaaaagta gtagattctt ttgaacaagc aattagctgg attaataaat
    20581 acagtagtca tcactcagaa gctatcatta ctaataatat cagtagggca gaaatttttc
    20641 aggatatggt agatgcagcg gcagtttatg ttaatgcttc aacacgtttt acagatggtt
    20701 tcgtttttgg actcggagca gagattggta tttcgactca aaaattacac gcgcgtggac
    20761 cgatgggatt agaagcttta acaagtacaa aatactatat taatggaact ggtcaagtaa
    20821 gggaatagtt gacctataat aggagagatt gcatttatcg gtctctcttt tacttttcta
    20881 aagtttagga tttatggtac aattaaagtt atgacaaacg attttcatca tattactgtg
    20941 cttttgcacg agactgtgga tatgcttgat attaagccgg atggtaccta tgtagatgct
    21001 actttaggtg gggcaggaca cagcgaatat cttctttcac aattaggccc agatgggcac
    21061 ctttacgcat ttgaccaaga ccagaaggct attgacattg ctcatatacg tttaaagaaa
    21121 tatgtggata ccggtcaagt aacctttatc aaagataatt tcagaaatct atcttctaac
    21181 ttaaaagcat taggagtgtc ggaaattaat ggtatttgtt atgatttagg tgtatcaagc
    21241 ccacagctgg acgagcgtga acgcggtttc tcatataaac aagatgcccc ccttgatatg
    21301 cgaatgaata gagagcagag ccttacagct tatgacgttg tgaacactta tagttatcat
    21361 gatttagtaa ggattttctt taagtatggc gaagataaat tttcaaaaca aattgctcga
    21421 aaaattgagc aagtacgagc tgaaaaacca atatctacaa caacagaatt ggctgagatt
    21481 atcaaatcgt ctaaatctgc caaagaactg aagaaaaaag gtcacccagc taaacaaatt
    21541 tttcaagcta ttcgtattga agtcaatgac gaactaggag ctgcagatga atctatacaa
    21601 caagcaatgg acttattagc tgttgatggt cgtatatctg tcatcacttt tcattcttta
    21661 gaagatcgtc ttactaagca actttttaaa gaagcatcaa cagtagaagt acctaaaggt
    21721 cttcctttta taccagatga tttacaacct aagatggagc tcgttaatcg taaacccatc
    21781 ttaccaagtc aagaagaact agaagcgaac aatcgtgccc attcagcgaa attgagagta
    21841 gcgcgtcgta ttcgataata agaaagaaac ttatgacaaa tgaaaaacga actgaagcag
    21901 taacacaaac cttacaacgt cacatcaaga ctttctctcg cattgagaaa gctttttatg
    21961 gcgctattgt aattactgct attatcatgg cagtcggtat tatttacttg caaagtaata
    22021 gtttacaagt taaacaagaa gtaaatcaat taaatagtaa aatcaacgat aaacagacag
    22081 aatttgacaa tgctaaacaa gaagtaaatg agctttctaa tcgtgatcga attactaaaa
    22141 ttgctaaaga tgctggttta acaattcaaa acgacaatat ttatcggaag gtggactagt
    22201 ctgtgacttt ttttaaaaag ctaaaaaaaa tcttccttga ttatgtgatc catataagag
    22261 ataggcgttc gccgcagaaa aatagggaaa gagtaggtca aaatctgatg attttaacca
    22321 ttttcctatt tttcattttt atcatcaact ttgtcatcat cgttggtaca gatagtaaat
    22381 ttggggttaa tttatccaag gaagccaaga aagtctatca acaaagtatg acagtacagg
    22441 cgaagcgtgg gacaatttac gaccgtaatg gaaatcctat cgctgaggat gctacgacct
    22501 atagtctcta cgctattatc tcaaagaact atacgacagc tacaggtcaa aaactatatg
    22561 tacaaccttc tcagtatgaa aaagtagcta gtatcttaga gaataaattg ggcatgaaga
    22621 aaaatcttgt cctaaaacaa ctcaatcaaa agaaattgtt tcaagtttcc tttggaagtt
    22681 caggttcagg attgtcttat accaagatgg ctgacatcaa gaaaacgatg gaaaagtcag
    22741 atatcaaagg aattggtttt tctaccagtc cggggcgtat ttatccaaat gggatatttg
    22801 cttcacaatt tataggattt actcttcctc aggatgatgg agatggaaaa aaattagttg
    22861 gtaatacagg ccttgaagcg gcacttaaca aggtattatc aggtactgat ggcaaagtaa
    22921 catacgaaaa agaccgtagt gggaatgttc ttttaggtac tgcaacaaca gaacgtaggg
    22981 cggttaatgg taaggatatc tacacaactt tatcagagcc tattcagact gttttagaga
    23041 ctcagatgga tgttttcgca gaaaaaacca aaggaaaatt tgcgagtgct acagttgtca
    23101 acgctaaaac gggagaaatt ctagcaacga gtcagcgtcc tacttataat ccttctaccc
    23161 ttaaaggtta cgataagaaa aatttgggta cctataacac actactatat gataattttt
    23221 ttgaacctgg ttcaacaatg aaggtaatga ctctagcctc tgcaattgat tctaagcatt
    23281 ttaactctac tgaagtgtat aacagtgcac aatataaaat tgcagatgcg gttattcgag
    23341 attgggatgt caatgaaggg ttatcatctg gtagttatat gacttttcca caagggtttg
    23401 cacattccag taacgttggt atggtaacct tggaacaaaa aatgggacga gataaatggt
    23461 taaactatct ttctaagttt aaatttggtt acccaacacg ttttgggatg cttcatgaaa
    23521 gtggaggttt gttcccatct gacaatgaag taactatcgc tatgagctca tttggtcaag
    23581 gtattggtgt aacgcaagtt caaatgttgc gcgcatttac gtctatctcg aatgatggtg
    23641 ttatgttaca accacagttt attagcagta tttatgatcc aaatacaggg acttctcgta
    23701 cagcacgaaa agaagtcgtt ggaaaaccag tttccaaaga agcagctagt aaaacacgtg
    23761 attatatggt tactgttggg acagatcctt attatggtac attatacgca gcaggtgccc
    23821 cagttattca ggtggggaat caatcagttg cagtaaaatc tggtactgcc caaatcgccc
    23881 aggaaggtgg tggtggttat ctgcaaggaa aaaatgatac tattaactca gttgtggcta
    23941 tggtgccatc ggaaaatcct gacttcatca tgtatgttac catccaacaa cccgaaaaat
    24001 ttagtatcac attctggaaa gatgttgtta atcctgtttt agaacaagca acagcaatga
    24061 aagaaacgat attaaaaccg gtattgaatg actcagaaca tcagacaaaa tataaactat
    24121 caaaaatagt tggtgaaaat ccaggtcatg tagcggaaga attacgtcgt aatctggttc
    24181 agccaattat tttaggaaat ggtagtaaag tttctaaagt atctaaaaga ccaggagcta
    24241 atcttgctga gaatgaacag ctactagtat taacaaataa actgactgaa ttacctgata
    24301 tgtatggttg gtctaaagca aacgttgaac agtttgctaa atggacaggt ataaaagtga
    24361 cctataaagg ttcaacctca ggtaaagtaa gaaaacaaag tattgatgtt ggaaaatcca
    24421 tcaataaaat taaaaaaatc aaaattacaa taggagatta acatgttttt aagtataatg
    24481 gcaggtgtca tagcatttgt cctgacagtt attgccattc cacgcttcat taagttttac
    24541 caattgaaga aaattggcgg gcaacaaatg catgaagatg tcaaacaaca tctagccaaa
    24601 gcaggtacgc cgacaatggg aggaacggta tttttggttg ttgctcttct tgtctcactt
    24661 atttttagta ttattttatc taaagaaaat agtggtaatt taggtgcaac ttttggtatt
    24721 ttatctgttg ttctcatcta tggaattatt ggttttttag atgatttttt gaaaattttt
    24781 aaacaaatca atgagggctt gactccaaag caaaaaatgt cgctccaact aattgcgggt
    24841 ctaatttttt attttgtcca tgtgttacca agtggcacat ctgcgataaa tatttttggt
    24901 tttaaccttg aagtaggata cttatacgct ttttttgttc ttttctgggt tgttggtttt
    24961 tcaaatgctg tgaacctaac agatggaatt gatggtctag cttctatttc ggttgttatc
    25021 agtttgatta cttatggaat cattgcttat aatcaaacgc aatttgatat tttattgatt
    25081 atcgttatca tgattggtgc tttacttggc ttttttgtct ttaatcataa accagctaaa
    25141 gtctttatgg gagatgttgg ttcacttgca ttgggtgcta tgttagcagc catttcaatt
    25201 gccttacgtc aggaatggac cttgcttttt atcggctttg tctatgtttt tgagacgtct
    25261 tcagtgatgt tgcaagtagc atactttaag tatactaaga agaaaactgg tgttgggaaa
    25321 cgtatcttcc gcatgacacc atttcatcac catttagaat taggtggtgt gtctggcaaa
    25381 ggtaataaat ggagcgaatg gaaagtagat gcttttttat gggctattgg tattttcatg
    25441 agcgctatta cacttgctat tttatatctt taaactttat taggggatgt ggtatggact
    25501 gccagattcc cttttgcttt cttgtagcct ttaatggtat aatgaacata ataatagtaa
    25561 ggaaatagac atgtcattta aagattttaa ttttaaacca tacattcaga gggctctcga
    25621 tgagttaaaa ttcgtcgatc caactgatgt tcaagctaaa ttgattccag ttgttcgttc
    25681 aggccgcgat cttgtggggg aatcgaaaac tggttctggt aaaacccaca cctttttact
    25741 tcctattttt gagaagctag atgaaagtag tgatgatgtt caagttgtta ttactgcacc
    25801 aagtagagag ttggcaacac aaatttatca ggcaactaaa caaattgctg agcattcaga
    25861 gcaagaaata cgggtggtta actatgttgg tggtactgac aaattacgtc aaattgaaaa
    25921 gttgaaagta agccaacctc atattgtcat tggaacccca ggacgtattt atgatttggt
    25981 gaagtcaggt gatttagtta ttcataaggc gcatactttt gtggttgatg aagcagatat
    26041 gactctggat atgggattcc ttgataccgt tgataagatt gcagggtctt tacccaagga
    26101 tgttcaaatt ttagtctttt cagcaacgat tccacaaaaa ttacagccat ttttaaagaa
    26161 atatttgacc aatccagtaa tggaaaaaat caaaacagca acagttatcg ctgataccat
    26221 cgataactgg cttttgtcca ctaaaggacg tgataaaaat gcacaaattt tggaactctc
    26281 taaactaatg cagccttacc ttgcaatgat atttgttaat acgaaagagc gtgcagacga
    26341 attacactcg tatctttcta gtaatggctt aaaagtagct aaaattcatg gtgggattgc
    26401 acctcgtgaa cgtaaacgta ttatgaatca ggttaaaaac ctcgaatttg aatatatcgt
    26461 tgcaacagat ttagcagcgc gtggtattga tattgaaggt gttagccatg ttatcaatga
    26521 tgccatccct caagacctat ctttctttgt ccaccgtgtc ggtcgcacag gccgtaatgg
    26581 cttatctggt actgctataa ctttatatca gccaagtgac gattctgata ttcgtgaact
    26641 tgaaaaacta ggtattaatt ttattcctaa agtcattaaa aacggtgaat ttcaagatac
    26701 atacgaccgt gatcgtcgta ataaccgtga aaagtcctat caaaaattag atacagaaat
    26761 gattggactt gtgaaaaaga aaaagaaaaa aataaaacca ggttacaaga aaaaaattca
    26821 atggaaagtt gaagaaaagc gtcgcaaaga gcgtagagca agtaatcgtg ctaaggggcg
    26881 tgcggagcgc aaagctaaaa aacaaagttt ttaaaattat taaagtctga aatcagttcg
    26941 tttcaggctt ttttattgat ataaaatctt tatcacctga taaggaaata ctattataca
    27001 gatagatttt agagtgttag actagtaata tgatatttta ggagaattct tatgtttaaa
    27061 aaaataatat tatctattgc aactatagcc gcaaccgcca gcctagcagt atcagttcag
    27121 gcgtcagaga aagtagaact taaagtagct acagattctg acacggcacc atttacttat
    27181 caaaaagacg ggaaattcaa aggttatgac gttgatgttg tcaaagctgt ttttaaaggt
    27241 agtaagtaca aagtaacctt caagacagtt ccttttgata ctatttcaac aggtattgat
    27301 gcagggaaat ttgatttatc agctaatgat ttttcatata ataaagaaag agcagaaaaa
    27361 tatctcttct cagatcctat atcccgttca aattatgccg tagtagggaa gaaggggagc
    27421 cattacaaat cattaagtga cctctctgga aaatcaacag aagttttatc tggcgttaac
    27481 tatgcacagg ttctagaaaa ttggaataaa aatcatccta ataaaaaacc aataaaaatc
    27541 aaatatgttt ctgggacaac tggtgttact agcagattaa aaaatattga gagtggaaaa
    27601 attgacttta tcctatatga tgccatttca tctgactata ttgtaaaaga tcaatcatta
    27661 aacttaagcg tttctccttt gaaaggtaaa attggtaata ataaggatgg attagaatac
    27721 ctccttttac caaaagataa aaaaggtaaa actctacaga aatttataaa taagcgtatt
    27781 aaagttttga aagaagatgg tactttggca cgtttaagta aacaatattt cggtggagat
    27841 tacgtttcaa acattgataa ataaatcagt gagactataa gttttatctt tttacatgtg
    27901 agagtttgcg tgatagaata acgaagataa gaaagtttag aaaaggaaag tagaaggaat
    27961 gaatcaattt atcttgacgg gtggatggtc ttggtataat aatttggtta gtcaggttcc
    28021 agcggggaaa ttatttagtt ggaaggctgt tttagatgca ataccctcaa ttttagagcg
    28081 attacccata acgctattat tgacagtagc aggagcctta tttggactca ttttagcttt
    28141 gatttttgca gtagtgaaaa ttaaccgtgt caaaatttta tatccgattc aagctttatt
    28201 tgttagtttt ttacgtggga ctccaatact tgttcaatta atgctgagtt attatggaat
    28261 tccacttttc ttgaaatttt taaaccaaaa atatggtttt gattggaaca ttaatgctat
    28321 accggcttct gtatttgcta ttactgcatt tgcttttaat gaagcggctt atacaagtga
    28381 aactatacga gcggcgattt tgtcggttga tcaaggtgaa attgaggcag cacgtagttt
    28441 agggatgacc tcagcgcaag tttatcgtcg tgttattatt ccaaacgcag cagtagtcgc
    28501 aacacctact ttgataaaca cgttaattgg tctaactaag ggaacctctt tagcttttaa
    28561 tgctggtatt gttgaaatgt ttgcccaagc acaaattatg ggtggtagcg attatcgcta
    28621 ttttgaacgt tatatctctg tggcacttgt ttactgggcg gttagtttct taattgaaca
    28681 attgggaaat gctattgaac gaaaaatggc aattaaggct ccacaccatt taactgatga
    28741 gattccagga ggagtacgct aatgataaaa ttgagacaat tgactaaatc attttctggt
    28801 caaaaagtgt tagacaaact tgatttagat attgaaaaag gtcaagttgt tgctctagta
    28861 ggtgcttcag gtgcaggaaa atcaaccttt cttcgtagta tgaattattt agaagagcct
    28921 gattatggaa caattgaaat tgatgatttt aaggttgatt ttaaatcaat cagtaaagat
    28981 gacatactaa cattacgccg taagttagcc atggtttttc aacaattcaa tctatttgag
    29041 cgtcgtacag ctttggataa tgtaaaagaa ggtttgaaaa ttgtcaaaaa gatgtcagat
    29101 caagaggcaa cacggattgc aagagacgaa ctggcaaagg tcggacttgc agatcgtgaa
    29161 aaatattatc caagacattt atcaggtgga cagaaacaac gtgttgcttt ggcacgcgca
    29221 ttagccatga aacctgatgt tcttctttta gacgaaccga catcagcact tgatccagaa
    29281 ttagttggag aagttgaaaa atctattgca gatgctgcta agcaggggca aaccatggtg
    29341 cttgttagtc atgatatgaa ttttgtttac caagtagcag ataaagtttt atttttggaa
    29401 aaaggtcgta ttttagagtc tgggacgcct gaacaattat ttaaccatcc tttagaagaa
    29461 cgtacaaaag aattcttcgc tagttataac aaatcgtacc tataagctaa aatattttga
    29521 tataatgaag gttagaatgt ctaatcttca tttttaatta ttcttgagtt aggctaagta
    29581 tataagaaag ctgcgatatg ttttgacaat cgcttgttgg atagagatgt ttttagaaat
    29641 agcaaaagtt tatttacaag gtttagggct tgccagcata actgttattt tagttggtat
    29701 attttgggtc ttttggcgtg ccaagaaaaa catagataaa acagctagag aacgccaatc
    29761 tgttctttat gatgtccttc tgatagctct tatgacagct ccaatacttg cctttgcatt
    29821 tgtagctatc attttaatga taaaagctta gtcagattag ttggaaatat agacgctttg
    29881 ctatagaata aaaccataag attcaaagga gaaatagtaa tgtatgatac tttaataata
    29941 ggttcaggtc ctggtggtat gacagcagcg ctttacgctg cacgttcaaa ccttaaagtt
    30001 gggttaattg aacaaggtgc tccaggtgga cagatgaata atactgctga aattgagaac
    30061 tatccaggct acgatcatat ctcaggtcca gaattatcaa tgaagatgta tgagccgttg
    30121 gaaaaatttg aagtagagca tatttatggt atagttcaaa gagttgagaa cgatggtgat
    30181 gtaaagcgtg ttatcaccga agatgaatcg tatgaagcca aaacagtaat ccttgctacc
    30241 ggagctaaaa acagcctttt aggagttccg ggtgaagagg aatatactag tcgtggcgtt
    30301 tcttactgtg cggtttgtga tggtgcattt ttccgtgatc aggatctttt agttgtgggt
    30361 ggcggtgatt ctgctgttga agaagctgtc ttcttaactc aatttgctaa aagtgttact
    30421 attattcacc gacgtgatca attgagagct caaaaagtac ttcaagaccg agcttttgct
    30481 aacgaaaaaa taaaatttgt atgggattca gtggtcaaag agattaaagg aaatgagatt
    30541 aaagtatctg gtgtaactgt tgaaaatcta aagactggag aaatctcaga gatgactttc
    30601 ggaggtgttt tcatttacgt tggtttgaaa ccacattcta gcatggtctc agaattaggt
    30661 attacggacg agactggctg ggtcttaact gatacaaata tgaaaacatc aattccagga
    30721 ctatatgcta ttggggatgt tcgtcaaaaa gatctacgtc agattgcgac tgctgttggt
    30781 gaaggtgcta tcgctggaca gggtgtttat aactatataa ctgagcattt ctaagaaata
    30841 ctaaaagtag aaaagactat cgaaaaacga ttagtctttc tttttatttt catgaaagta
    30901 aggtgataaa ctgaactacc gagatagaaa tatgctataa tgttagacat atagccttat
    30961 cctaggctca tgattaaaag gagattgtct gatgtataaa gacgatagtt taacactaca
    31021 tactgattta tatcaaatta atatgatgca agtctatttt aataaaggta ttcacaataa
    31081 aaaagcggtg tttgaagctt actttcgcaa ggttccattt gaaaatggtt atgctgtttt
    31141 tgcaggttta gaacgtattg ttcgttattt agaaaatctg agcttttctg atagtgattt
    31201 atcctattta gaagagttag gttatcctga agaattttta gattacttaa aaaatttaaa
    31261 aatggaactg actgtcaaat cagctaagga aggtgacctt gtatttgcta atgaaccatt
    31321 agttcaaatt gaaggtcctt tagcacaatg tcagttagta gagacagcga ttttaaatat
    31381 catcaactac cagacacttg ttgctacaaa agcagctcgt attcgttctg ttattgaaga
    31441 tgaaccgcta ttagagtttg ggacacgacg tgctcaagaa atggatgcag ccatttgggg
    31501 tactagggca gcgattattg gtggtgctaa cgcaacgagt aatgttcgtg ctggtaaaat
    31561 ctttaatatt ccagtatcag gaacgcatgc acatgcttta gttcaaactt atggcgatga
    31621 ttaccaggca tttaaagctt acgcagaaac tcataaagat tgcgtctttt tagtggatac
    31681 ttatgatacc cttcgtgtag gtgtgcctaa tgctattcga gttgcaaaag agatgggcga
    31741 aaagattaat ttcttgggag ttcgccttga ttcaggtgat ttagcttatt tgtctaagaa
    31801 agttcgtcag cagttagatg acgccggttt tcctaatgca aagatttatg cttcgaatga
    31861 tctagatgaa aatacaatcc tcaacctaaa aatgcaaaaa gctaaaattg atgtttgggg
    31921 tgtcggaaca aaattaatta cagcctatga tcagcctgcg ctaggtgctg tttataaaat
    31981 tgtttctatt gagactgacg ccggtagtat gcgagataca attaaactat caaacaatgc
    32041 ggaaaaagta tcaacacctg ggaaaaagca agtttggcgt atcaccagtc gagctaaagg
    32101 gaaatctgag ggtgattaca tcacatttgc agacactgat gtgacacaat tagatgaaat
    32161 tgacatgttt catccaactt atacttatat caataaaaca gtccgtgatt ttgacgcagt
    32221 acccttactg gtagatatct ttgacaaagg gaaattagtt tatcaattac caagccttca
    32281 agaaattcaa gagtacggac gcaaagaatt tgatcagctt tgggatgagt ataaacgtgt
    32341 tctgaatcca caggattacc cagttgattt ggcacgcgat gtttggcaaa ataaaatgga
    32401 cctgattgat cgtatccgta aagaagcttt ggcaaaagga gaagtaagat gactttgcaa
    32461 gatcaaatta tcaaagaact cggtgtaaaa cctgttatta atccaagtca agaaatccgt
    32521 cgttccgtag aatttttaaa agactatcta ttaaaacata gttttctaaa gacctatgta
    32581 ctgggtattt caggggggca agattcaact ttagcaggac gtctagctca actagcagtt
    32641 gaagagttgc gtgctgatac aggggaaaac tatcaattta tagctattcg tttaccatat
    32701 ggtattcagg ccgatgaaga agatgcacaa aaagcattag attttatcaa gccagatatt
    32761 gctttgacca ttaatattaa agaagctgtt gatggccaag ttagagctct taatgcagca
    32821 ggagtagaga ttacagactt taataaagga aatattaaag ctcgtcaaag aatgattagt
    32881 caatatgcag ttgcaggtca atatgctgga gcggttattg gtactgatca tgctgcagaa
    32941 aatattacag gttttttcac caaatttggt gatggaggtg cggacttatt acctctcttt
    33001 agacttaata aaagtcaggg aaaacagtta ttagctgaac ttggtgctga taaagacttg
    33061 tacgaaaaga ttccaaccgc agatttagaa gaaaataaac caggaatcgc tgatgaaata
    33121 gcacttggag taacctacca agaaattgat gcttatcttg aagggaaggt agtttcggac
    33181 aagtctcgag gtatcattga aaattggtgg tataagggtc aacataaacg tcatcttcca
    33241 atcacaattt ttgatgattt ctggaaataa gaaaaatagc tagaatattc tagctatttt
    33301 tgtttctcta ataaattact ttcagacttg cagaaatatt cagaatgttt ataatagtag
    33361 ctaagtataa aaggaggaat ttatgtcaga attaactcaa acatttacag ataagttatt
    33421 agctgattac caagcaaata caaaatttag cgctattgaa aatgcagtaa ctcacaatgg
    33481 tttattgaag tctctagaga ctcgtcaaag tgagattgag aacgactatg tcttttcaat
    33541 tgatttgacc aaagatgaag tctctaatca gaaacaatca ggacgttgct ggatgtttgc
    33601 tgcactgaac acatttcgtc ataaactgat ttcagatttt aaactcgaga actttgaatt
    33661 atctcaggca catacatttt tctgggataa gtatgaaaaa tcaaattggt ttatggaaca
    33721 aattattgcg actgcaaatc aggaattgag tagtcgtaag gtaaaattcc tattagatgt
    33781 acctcaacaa gatggcggac aatgggacat ggtggttgct ctgtttgaga aatatggtgt
    33841 cgttcctaaa acagtttatc ctgaatctgt ttcatcaagt gctagtcgtg aactaaatca
    33901 atatttgaat aaacttttac gccaagatgc acagattcta cgagaattaa tagcgcaggg
    33961 agcagatggg gcaactgttc aaaataaaaa ggaagaattg cttcaagaaa tttttaactt
    34021 tttagcaatg aaccttggct tgccaccgca atcttttgat tttgcttatc gtgataaaga
    34081 caatcattat cagtctgata aaaatattac accgaaagca ttttatcaaa aatatgttaa
    34141 tcttgacata tcagattatg tttctattat caatgctcca actgtagata agccttatgg
    34201 acaatcctat acagttgaga tgcttggaaa tgttgtcgga ggcccagctg ttaaatattt
    34261 aaaccttgat atgaaacggt ttaaagagtt agcgattgcg caaatgaaat caggagaaac
    34321 tgtgtggttt ggttcagacg taggtcaagt atcaaatcgt caaaagggta tcctagcaac
    34381 tacgacttat gattttaatt catctatgga tattaaactt agccaagaca aagcgggccg
    34441 cctagattat agtgaaagtt taatgacaca tgctatggtc ttaacgggag tagaccttga
    34501 tgaatctgga caacctttaa aatggaaagt tgagaactct tggggagaga aagttggtaa
    34561 ggatggttat tttgtagctt cagatgcttg gatggatgaa tatacttacc aaatagtcgt
    34621 tcgtaaagaa ttgcttacta aagaagaatt agaggcttac aacgcagagc caattacttt
    34681 agcgccgtgg gatccgatgg gagcactagc taattagtaa tagtaattat aaattaaaaa
    34741 actggatata ttatccagtt tttttaatta ccgttaggtg ctgtattgtt attaccatta
    34801 ccatgtccag ttcctggcac agtttgattg gaattattag gggtagtagc attaccatta
    34861 ttaggtgttt cgacttgtgg ggatgatgaa ttggcatcat ctgctgtatt cttatcctct
    34921 ttttgtttct cagaggatgt tgaactactg ctttgacttg actgtccaga attttgatag
    34981 atattcttat agacagaaga agagtaagtg ccggttgtag ttgtcccatt gatatacaag
    35041 tagcttccac ttcgatagag cccttctggc attgtccaat ctgcactgta tccgccagtt
    35101 aggtaggaca tcattgcacg ataaacctca gtagcaatat ccagttggct accataaagt
    35161 ggtgtaaggc gatttttata accagtccaa attgccattg tgtacttaga agtatagccg
    35221 acaaagtttt cgtcaggagc cattgtacca acggcgctat tgtagatacc agtagttgct
    35281 tcaattttag ctaactcatc ttctgtatag ttggaagtac cagtcttacc agcttgtgca
    35341 acaccaggga tagctgcttt agtaccagta ccaaatgtta gtactgtttt caacatatcc
    35401 gtcatcatgt aggcagtagt ctctttcatc gcacggctac cagacgctgc ataagtatca
    35461 ttggttccat cgctaaattc aattttatta acatattgcg gcttatagta agttccgccg
    35521 tttgcaaaag ccgagtaagc cgctgccatt ttctcactac ttgcaccata ttttgcatca
    35581 ctgctattgt tgttacttga aatagcattt gaatagttca tttctggata gtatatgcct
    35641 aatttttcaa ggaaagattt tgcttcgtct aatccagcgg cttcaagtgc tctgacagca
    35701 gggacgttac gtgattgttg aatagcggtc tgcatactca tccaacccat atattgacga
    35761 tcccagtcat atagttgagt agaagtacca ggccagtagt aaactgagtc gtttaatgat
    35821 tgacctgttg aattatagac accactatca atagcaggtg cataagctga gataggtttc
    35881 attgtagaac cccaatcgcg gtctgttaag acagattgat ttgtcccaaa tgaaatattt
    35941 tcattctgat gacgcccgcc taattgtgca atgactttac cattagtcgc atccatgatg
    36001 gtagatgcta tttgtaattc attgtttgga taagcgatgt aagtatcact gttgtagatg
    36061 tcatatagtt gtttttgtgc atcagtgttg atattagtat acacttttag cccagcagta
    36121 aagatatctt taccagtttt ttgtttaact tcactaataa cttgttttaa gtagttatcc
    36181 atatattttg gataagtaga tttttgcttt aattctttta agccatcagt tactggagtt
    36241 gcaacagctt ggtcgtattc ctttttagaa atgtttttat cttgatacat ctgctgaaga
    36301 acggtattac gtcttgtttg agcagattct gggtttttat aagggtcata ttgtgtaggt
    36361 gcttgaggaa taccagcgag caaagcaagt tgtgcaatag ataattcctt taggtcttta
    36421 ccaaagtatg atttagctgt tgttctcata ccataattcc catttcccat ataaacttta
    36481 ttaatataga aagtaagaat ttcttctttg gtgtatttac gctccatttg aagcgcaagc
    36541 caaacttcct gtgatttacg ttttaacgtt tggtcagatt tattggtaga aaagtaagcc
    36601 agtttaatca actgttgatc aagggttgaa ccaccttgcg tattactact aacaaggtta
    36661 tgccaagctg cacctaaaat acgataaata tcgacacctc tatgtttaaa gaaacgttta
    36721 tcttctatag aagtgatagc gttaaccaaa tttagtggaa tgctatccgc actaacactt
    36781 tcacgctttt ctgagcctaa atcggcaata agtttattat taccatcata aaccaaacta
    36841 gagttaacgg attttaaagc ttgatcggtt aatttcggag aacgactaac gtagtatgca
    36901 aataggagcc cacctattac aatagctaag ataataaatc ccattataat gccaaaagca
    36961 tacttcaata gtttgataac agattccttt ttaattgtaa tcaccgccta ataatttttc
    37021 ttcaataata tctaagtaag ggacttgagg aaaggcactc tcctttacga aaaatccatt
    37081 ttttctgata taatcaatag gcattgattt attgccttta tcaatctgat aaaatgaaat
    37141 taactcatta gcagggagta gataggtttc cttaagtgtg gaaaaatgaa gcaagacaaa
    37201 gcaaatccct ttttgctgta atacatttgc catgtgctct atttggtgag catgaaaatt
    37261 tttcataggc atagcagttt tctgccgggt ttctttggct tcaaagtcaa tatagtaacc
    37321 tttgtaaaca ccagaataat cagtagtaga agcttgtcta aaataagctt ctacaatctt
    37381 ggcacgacta cgtttaggat aatctacctt gacgatttgt actggcgttg gtttcttatg
    37441 aatgacagct aattcgtgag acaaatagta gtcattggtt gcattgatag cagcttcaaa
    37501 tgacatacca cggttggcaa aatcgatttt ctttttcttt gattttgtaa ctgttgtttt
    37561 ccgaataagc tgatgtggat agttaaccat ttttctcctt gcattacaaa taaaatgcta
    37621 agataagcaa aactattata ccataaaatt aggatggaca acaaatgaag tcaactattc
    37681 ttgtaacagg ttacaaaaat tttgaattag ggattttcca ggataaggat cctcggatta
    37741 ctattataaa aaaggctatt gataaggatt ttagacgctt cttggaaaat ggagcagact
    37801 ggtttatttt tatgggaaat ttagggtttg agtactgggc tttggaagtt gctcttgatt
    37861 tacaaaaaga atatgatttt caaattgcga caatttttac ctttgagaac catggccaaa
    37921 attggaatga ggctaataaa gctaagttgg cactctttaa gcaagttgac tttgttaaat
    37981 atactttccc aagctatgaa aacccgggtc aatttaaaca atacaatcat tttctcatta
    38041 acaacacaca aggtgcatat cttttttatg attctgaaaa tgaaactaat ttaaaatttt
    38101 tgctagaaat gatggaaaaa aaggaagctt atgatatttc tttcctaact tttgatcgct
    38161 taaatgaaat atatgaggaa tgatcgtttt ttcggatatg agaacgtttt acgcttgatt
    38221 tttccattta atttaggtat tatttacttt gatataaagg ataggactta tattaaaacg
    38281 atttgactgg tttggagaat agaatggcaa gtattattta tagcccaaaa gatatttttg
    38341 agcaagattt taaagttagt atgcggggat atgacaagaa agaagtcgat gtatttctgg
    38401 atgatgtcat caaagattac gaaaattatc ttgagcaaat tgagaagctc caaatggaaa
    38461 atagacgtct acaacaagct ttagacaaaa aagaaagcga agcttcaaat gtaagaaatt
    38521 cagggacagc tatgtataat caaaaaccga ttgcccaatc tgcaaccaat tttgatattt
    38581 taaaacgcat tagccgcctt gagaaagaag tttttggaag acaaattaga gaataaggat
    38641 ttctcaaata tgcaattttt ggataatcgc gtagtattga tatactatga ggaaagtcca
    38701 tgctagcact ggctgtgatg ccagtagtgt ttgtgctagg cgaaaaaata agcctaggga
    38761 gatagctagc tatcttacgg caagcaaaag ggctaagtct ttggatatgc ctgaatagct
    38821 ttgaaagtgc cacagtgacg tagtttctag ggaaatctag aaagtggaac gcggtaaacc
    38881 cctcaagcta gcaacccaaa ctttggtagg ggcatgggat agttggaatg agaacaatct
    38941 atcctgactg ctttgcagta gacagatgat tatcgaaaga aacaattcct agttgtttct
    39001 ggaacaaaac atggcttata gaaaattgca taataggtta gtggctgaga taacatctca
    39061 gccaattttt aactgtgatg aatacgatag tgtaaaagag gaaaaatgaa agaatcattc
    39121 aaattaatag caactgctgc tgctggttta gaagccattg ttggtagaga aatcagaaac
    39181 ctaggaattg actgccaagt tgagaacggt agagtccgtt tccacggaga catcaaaaca
    39241 atcattgaaa cgaacctatg gctacgagct gccgatcgta taaagattat tgtaggagag
    39301 tttccagcac gtacttttga ggaacttttt cagggtgtat atggccttga ctgggaaaat
    39361 tatttaccgt taggggcaaa atttcctatt gctaaagcaa agtgtgtcaa gtcaaaattg
    39421 cacaatgaac ctagtgtaca agccatttct aaaaaagctg ttgctaagaa acttcaaaaa
    39481 gtctttcacc gtcctgaagg agttccctta caagaaaatg gagctgaatt taaaatagaa
    39541 gtttctattt taaaagataa agcgactgtc atgattgata cgactggatc aagtctcttc
    39601 aaacgtggtt accgagcaga aaaaggtggt gcgccaatta aagaaaacat ggcagcagct
    39661 attattcaat tatctaattg gttccctgat aagccattga ttgatccaac ttgtggttca
    39721 ggtacttttt gtattgaagc cgctatgatt ggaatgaata tagctcctgg ttttaatcgt
    39781 gattttgcct ttgaggcgtg gccatgggta gatcaaagtc aagtacaaaa ggttcgcgat
    39841 gaagctgaga gtaaagcaaa ctatgatatt gatttagata tttcaggttt tgatttagac
    39901 ggtcgtatgg tagaaattgc tagaaaaaat gcagaagaag caggacttgg agatgttatc
    39961 aaattgaaac aaatgcgtct tcaagattta aaaacagata aaattaacgg tgttattatc
    40021 tccaacccac catatggtga acgactcctg gatgacaaag cagtggacat tttgtataat
    40081 gagatgggac aaacatttgc gcctttaaaa acttggagca agtttatcct aactagtgac
    40141 gaaggatttg aaaaaaaata cggctcacaa gctgataaaa aacgtaagct ttacaatggt
    40201 actttaaaag ttgatttgta ccaatattac ggtgaacgtg tgagacgtca agtaaaataa
    40261 cggggagaac tcatgtcaga agatcaaaaa cacccatttt ttgaaccaga aaaagaaact
    40321 gatggtcttg aatttaaaga tgctaaagaa atgacagtag aagaggctgt tagaaaagat
    40381 tctgaaatta aggctggtat taccgaagaa gatagtattc ttgataaata catcaaacaa
    40441 catcgtgatg aagtagcttc acaaaagttt gagacaaaat caagcgattt tgcaaacctt
    40501 gatacggcat ctttagatga ttttatcaag aaacagcgcg aagaattatc agcgatgtta
    40561 gctgcagaag agctttctaa aaagttggat aattcagtct ctcaagaaca agatatagaa
    40621 gctaatgctg tctcacctaa agaagaatct tcacaagaac aagaaaatag tgtaactcca
    40681 gttcctcctc tgaatacgga agcagaacca actgcaacag aaccagattc gactattgct
    40741 gattcagagg agtataaatc ctcttctaag aagcgtggag gtattgtagg aacattgatt
    40801 gctcttattt tacttctaat agtaacaatc tttggttaca actatttcaa gaataataat
    40861 tcgacaaatt ctcaaactgc aacaagtcaa agttctagtt ctaaggctac gacgacttca
    40921 tcagaagaag ataaaaaagc tagtcagaac ttagataatt tcaacaaagc ttatgctaac
    40981 ttttttgttg atgataagaa aacccaactc aaaaatagcg aatttgataa attgtcagaa
    41041 ttagaaaaga aagttgatgc tttaaaagga actaaatact acggtaaagt aaaagttaaa
    41101 tttgatagtc taaaacgtca aattgatgct gttaaagctg ttaacgacaa gtttaagtcg
    41161 cctgctgtag ttgatggtaa gaagagtgaa aaattagaag taaaagatgg agcgaatttt
    41221 gactctttag atagtaaaac tcttaatact ggtaatgcta gtctagatag tttacttcac
    41281 tccatcgttt caactggtcg taatcaggta aaacaatccg aagaacaggc taaaaaaact
    41341 caagaagaac agtcttcgtc taataaggtt tcagacactc aaacaactga acaaccaaac
    41401 gtaactaacg gtcaatcttc ctcatcagct gctacaataa ataatcaggc agctggcact
    41461 gcatcaggta atttggaacg taaccgtagc cgagtaccgt ataataatgc tgctattgcg
    41521 gacacaggaa atcctgcctg gatatttaac ccaggagtat tagagaaaat cgtggccact
    41581 tcacaggcgc gaggctattt ctctggaaat aactatattc ttgaacctgt aaatattatc
    41641 aatggtaatg gttattacaa tatgtttaaa ttagatggca cttatttgtt ctctatcaat
    41701 gccaagacag gttactttgt aggtaacgct cctggaagag ctgatagttt agattattaa
    41761 tataaagagc aaaaccagtt ttttgtctac accctaaaag ttagaaaatc agtctaacga
    41821 ttagggtgtt ttttgtattc gcatttagat gtgtacgact gctatgagga tattagggaa
    41881 aaattaaaag gattttgctc tctatgatac agaacaaaat cctttgttta aattaataag
    41941 acaacttttg gaattccaaa agttgacttt tattttttat tagatcagac aatgtggcgc
    42001 tcaaaagcat tgtcagaaat cccttgggaa agaattaatt tcgcccattc ttgagcagag
    42061 tggagactgt ggtctttata atttccacaa gattcaatgg ttgttccagg aacgtcttcc
    42121 caagtgaccc caccagcaat agcctctaga ctagatttga tgactttagc aatttcagta
    42181 gcatcctgtt ttccccacat aatcatgtga aaaccagttc tacaaccaaa aggagaacaa
    42241 tcaatcaaac cattaatttg ttggcggatt aacttcgcca gaaggtgctc gatagtatgt
    42301 aaaccagcag tatcaatagc attttcatta ggctgaataa ggcggatatc aaagttagta
    42361 atgatgtctc cgaccggtcc aacttcttct gaaattaaac gaacgtaggg tgctttaacg
    42421 atggtgtgat ctaattcaaa actctctacg acaacttctt ttgtcataag ttttctcctt
    42481 ttgtattaca ttctagagac taaaggagat gttgaaaatt tctcctaatc cctttaaata
    42541 acattctaat catatcataa atgtctataa ttttaaattg tgaaaacgcc ttttttatga
    42601 taaacttaaa agtggacttt ctatatgaaa tctagaaaaa aattgaggta taaacatgtt
    42661 taatattata ttagcaatgg tttgtgcact cattggtttg attattggtt atgtggctat
    42721 ttcaatgaaa atgaaatcat caaaagaagc tgcagaacta actcttttaa atgcagaaca
    42781 agatgccgtt gatttacgtg gtaaagcaga aattgaagca gaacacatcc gtaaagcagc
    42841 ggaacgtgaa agtaaagctc accaaaaaga attactttta gaagcaaaag aagaggctag
    42901 aaaatatcgt gaagagatcg aaaaggaatt taaatctgat agacaagagt tgaaacagat
    42961 ggaggcacgt ttaacagatc gtgcatcatc tcttgatcgt aaagatgaaa atctatctaa
    43021 caaagaaaaa atgttagata gcaaagaaca aagtctaacc gataaatcta gacatattaa
    43081 tgagcgcgaa caggaaattg ctactttaga aacaaaaaaa gtagaagaat tgtcacgcat
    43141 cgcagagctt agtcaagaag aagctaaaga tatcatttta gctgataccg aaaaagattt
    43201 agctcatgat attgcgacac gtattaaaga agcagaacgg gaagtaaaag atcgatcaaa
    43261 caagatagca aaagacttgc tagcccaagc tatgcaacgt ttagctggtg aatatgttac
    43321 tgaacaaaca attacaactg ttcatctccc tgatgataat atgaagggac gtatcattgg
    43381 tcgtgaagga cgtaatattc gtactctgga aagtttgaca ggtattgatg ttatcataga
    43441 tgatacgcca gaagtcgttg tcttatcagg tttcgacccc attcgtcgtg aaattgctcg
    43501 tatgacttta gaatctttga ttcaagatgg acgtatccat ccagctcgta ttgaagagtt
    43561 ggttgaaaaa aatcgtttgg aaatggatca acgtatccgt gagtatggtg aggcagcagc
    43621 ttatgagatt ggtgcaccaa atctgcatcc tgatttgata aaaattatgg gacgtttgca
    43681 gttccgtaca tcatatggtc aaaatgtact tcgtcattca gttgaagttg gtaaacttgc
    43741 aggtatttta gctggagaac taggagaaaa tgttgattta gctcgtcggg ctggcttcct
    43801 ccatgatatg gggaaagcta ttgatcgcga agttgaaggc agtcacgttg aaattggtat
    43861 ggaatttgct cgtaagtaca aagagcatcc gattgttgtt aatactattg caagccacca
    43921 tggtgatgta gaacctgaca gcgttattgc agtaattgtt gctgctgcag acgcccttag
    43981 ttcagcacga ccaggtgcgc gtaatgagtc aatggaaaat tatatcaaac gtctgcgtga
    44041 tttagaagaa attgctaatg gctttgaggg agtgcaaaat gcctttgcac ttcaagccgg
    44101 acgtgaaatt cgtatcatgg tacaacctgg taaagtttct gatgatcaag tagttattat
    44161 gtcgcataag gtacgtgaaa aaatcgaaca aaacttggat tatccaggta atattaaggt
    44221 aacagttatt cgtgaaatgc gagctgttga ttttgcaaaa tagtcaaaag gagagcttgt
    44281 tagctctcct tttgttatag agttgacatt tgtcatattt tgaaatgaca aaaatatcta
    44341 aaaagtttcc ttctgatttt ttatactatg attatagtaa aggagaacca aatggcaaaa
    44401 gatataatta aggttgatca tatatttaaa agcatcgggc aaaaaacgat tttagaagat
    44461 atatcattta gtatagcttc taaccaatgt gtagcattga ttggacctaa tggtgcaggg
    44521 aaaacaactc tgatgtcaac tttattaggt gatattagta tttcttcagg agcattgact
    44581 atttttaatc tgcctgcaca ccacaatagg ttaaaatata aggtggccat cttaccacaa
    44641 gaaaatgtat tgccctcaaa gtttacagtg agagaactca ttgatttcca aaggtgttta
    44701 tttccagaag tgctgccaat gtctctgatt ttagactatc tacagtggag tgatgcgcac
    44761 ttgcaacaat ttacagagac tttatctgga gggcaaaaac gacttttagc ttttgtttta
    44821 actttggttg gtaagcccca attactattt ttagatgaac ctacttccgg aatggatact
    44881 tccacacgtc aacgattttg ggagctggtt gcgacactaa aaaaagaagg tgacacaatt
    44941 gtctattcta gtcattatct cgaagaggta gaacatacag ctgataggat tttagtactt
    45001 cataaaggaa agttattacg cgatacaacc ccctttgcca tgaagcaaga aaaaaccgaa
    45061 aagttattca ccgttccgct tagttatcaa aaattattac ctacctattt gattacagag
    45121 tgtgaagcca agagtgatag tataacgttt gttactgggg aggctgaaac tgtatggaaa
    45181 atactggcag ataatggttg tcctattgaa gctattgaga tgaccaatag aactttgtta
    45241 aatcgtattt ttgagactac taaggaggta aaacatgaga atctttaaag cattattgaa
    45301 aatggaattt cttttgacaa aacgtcaact tgccaatctc attatggcta taggtatgcc
    45361 agtggctttt tttctatttt tttcaggatt tatgggcgaa ggactcacta aagccatcga
    45421 agctatctac gttcgtaatt atatgataac gatggcagga ttctcaagtc ttagctttgc
    45481 tttttttact tttccatttt ctatgaaaga tgatcaactc tcaaaccgaa tgcaattatt
    45541 aagacactca ccagtaccaa tgtggcaata ttatctagca aaaattattc gtattttatt
    45601 ttactattgc ctagcaataa ctgttgtttt cctaacaggg catattttaa ggcaagttag
    45661 tatgccaata gaacaatgga tgcagagctt tttattgcta ttaggaggag caacatgttt
    45721 tattcctttt ggattgctcg tctcctactt taaaaatact gaactaatgt caatggtagc
    45781 caatatttgt tatatgtcat tagctgttct tggtggcatg tggatgccaa ttacaatgtt
    45841 cccaaaatgg ttgcaaggtt tatcaaagtt aacccctact taccatttga ctcaagttat
    45901 ccttagtccg tttgctaata gttttgctgt tttttctctt attattctga taggctatgg
    45961 tattataatg ttagttatcg cttatctact atctcagaag aggcattcaa tttgaaaaat
    46021 aaaaaaaata actatgattt aatgtatttt attccgttag tatttttgat ttaccccata
    46081 ggagggattc tctattatca ctaccctttt tggactttat tttttacgct agcttttgta
    46141 ggagcatatt tatactctat tattatcagg ggcgaatcaa agtatcacat gattgcctgg
    46201 tttacaatgt tgacatatat tttctacatg acagttttta taaatagtgg tttcatctgg
    46261 tatatttact ttctgagtaa tttactcgtc tatcgcttta gagataaatt gaagtctttt
    46321 agattcatta gttttgcttg cactctagcc acaatggtat ttttatgctt ctttaaggca
    46381 tcagactttg gtgatagaat catgtttcta attgttccta ttttttgtat cggttacatg
    46441 tggattgcta ttgaaaatag aaatagcgaa gaacaaagag aaaaaattgc agagcaaaat
    46501 cagtatatca atattttgtc agctgaaaat gaacgtaacc gtatagggcg tgatttgcat
    46561 gatagtttag gacatacatt tgctatgatg acactaaaga cagaactagc gttaaagttg
    46621 ttagaaaaaa ggaactatga taaggtacaa aaagaactat cagaattaaa tcatattagt
    46681 catcaatcga tgagtgaggt ccgtcaaatt gtttctaacc tcaagtatcg tacagtagtg
    46741 gaagaaattg atgagttgta ccgcttgttt caattatcta acatcaagtt aacagttgtt
    46801 aataagttag agacgtcaca actaagtcca gtaacacaat ctacaatcac aatgatatta
    46861 aaggaattat ccaataatat tgttaagcat gctgaagctg atagcgtgga gttgagttta
    46921 gtaaggcaag gtgctactat aaatattgag atgattgata atggttgtgg ttttacaaac
    46981 cttgacggag atgagttgca tagtattcaa gagagattaa ctatcgttga agggacacta
    47041 acgattttgt cccgaagtaa accaactcat attcaggtga tattgaaaga ggtataagat
    47101 gaaattactt gttgctgagg atcagtccat gctacgagac gctatgtgcc aattactttt
    47161 gatggaagaa agtgtttcta ctattgatca agctggaaat ggtgaagaag ctattgccat
    47221 tttatccaat aaagccatag atgttgcaat attggatgtt gagatgccga tattatcagg
    47281 tttagatgtc ttagagtggg ttagaaaata ccagaatgtt aaggtcataa ttgtaacaac
    47341 ctttaagcgg tcaggttatt tccaaagggc tattcgttca aatgtagatg cttatgtttt
    47401 gaaagaccgc tcagtagctg atttgatgaa aacgattcaa aaagttttat ctgggggtaa
    47461 agaatattcg cctgaattaa tggaaaacgt tatatctaat cctttgagta agcaagaaat
    47521 aaaaattctt tccctaattg ctcaagggaa gaccagcaaa gaaattgcat gcactttatt
    47581 tttatcgaat ggtacagttc gtaattacac gtcaaatatt tttgataaat taggagttaa
    47641 taatcgtttg gaggcactta aaatagcacg agaaaaggat ttgctgtgat agtttttttc
    47701 cagtcgagat gccataatgt attaagaggg atatatgtat gcaaaaattt aaagaacgtt
    47761 ttgatacgct tagtgatgca attttggcca ttgctatgac gattttagtt ttggagataa
    47821 aaactccgac tacgatgggt gatattggtg actttacacg gaacataggt ttatttattg
    47881 ttagctttgt tgttgttttt aatttttggt acgaaagggc acaaaatagc ctagatgctc
    47941 aaaagactaa tgatgaaatt atagctcttg atatcattga acacctaggt atttgtttga
    48001 ttcctctctt tactaaattt atgatttctt ttgagaatca taattttgct gttatggctt
    48061 atggactctt aacgttatta gttggtctca catcagacat tattcggatt cgtttggcaa
    48121 gctatgattt agtgaccatt cctagtgaat tgaaagagcg ggttataaaa gtcatgacaa
    48181 cttttgccat tcgttcagtt gtggttcgct ttatcattat tatcctcgct tattttctac
    48241 ctgaggtagg gatatttgca tatttagtca taccgctctt tatgttttgg cgtcgctcta
    48301 gagcaggaaa atactatact aaaaagggca ttgaggcacc ctcttatatc aagcttttta
    48361 tccaaattgg agagagagtt cctgaggatt ttactaattc aagtagtgga ataagtgggg
    48421 taaaaagact ctataaaaaa taataggaga ccttgaaaaa aaggtttctt tttgttaaac
    48481 taatgagaag aaactttatt tagggagcaa tttatgaatt gctataaaag ttaatagtta
    48541 aggtaagaac cagattgttc tggtcttttt aacacttaac acccgatcta ttaatttgag
    48601 gagtgtgtaa tgtctgaacg tggtttgcta attgttttct caggaccatc tggtgttggt
    48661 aaggggacag ttaggcaaga aattttttca acccctgacc ataaatttga ttattccgtt
    48721 tcaatgacta ctcgtccaca acgcccaggc gaagttgatg gagttgatta tttcttccgt
    48781 acacgtgaag agtttgaagc gttgattaaa gaaggtcaaa tgcttgagta tgcggagtat
    48841 gttggtaact actatggaac tcctttaagt tatgtcaacg agactttaga caaagggatt
    48901 gatgtttttc ttgaaataga agttcaggga gcgcttcaag ttaaatcgaa ggttccggac
    48961 ggtgttttca ttttcttaac accaccagat ttagaggaat tagaagagcg tttagtagga
    49021 cgtgggacag atagtccaga agtgattgcc caacgtattg aacgtgccaa agaagaaatt
    49081 gctttgatga gggagtatga ctatgctgtt gtcaatgatc aagtgtcatt agcagcagaa
    49141 cgtgttaaac gtgtcattga agcagaacat tatcgtgtgg atcgtgttat tgggcgttat
    49201 accaacatgg tcaaagaaac tgataaaaaa ttaagttaaa aatagaaaag agaattgcct
    49261 tatgatgtta aaaccttcta tcgacacttt gttggataaa gttccttcaa aatattcttt
    49321 agttatttta caagctaagc gtgctcatga attggaagct ggtgagaaag ccactcaaga
    49381 ttttaaatct gtgaaatcaa cacttcgcgc tttagaagaa atcgagtcag gtaatgttgt
    49441 aattcaccca gatccatcag caaaacgtgc ctcagttcgc gctcgtatcg aagctgaacg
    49501 ccttgctaaa gaagaagaag aacgtaaaat caaagagcaa attgctaaag aaaaagaaga
    49561 cggcgaaaaa atttaagcaa gtcaaaagtg agaggttgaa acattctgta tcagcctctt
    49621 ttcatatttt agagaattag ggagggttta tggcaagaaa attagcacag gttattgttg
    49681 atattcctct gatgcagaca gataaaccat tttcgtatgc cattcctaaa gatttggaag
    49741 atttggtaca agtaggagca cgcgttcatg ttccttttgg aagagggaat cgtcttttgc
    49801 aaggctttgt tgtcggtttc agagatgatg atgaattaga gactaaggat attgcggggg
    49861 tcctagattt tgaacctgtt ttaaatcagg aacaactaga cttagctgat cagatgcgcc
    49921 atactgtctt ttcttataaa atatctattt tgaaatcaat gttaccaagt cttttgaatt
    49981 cgcaatatga taaactcttg ttagcaacag acacattacc atcagaagat agagaagatc
    50041 tatttggtca taaaacggaa attgtctttt caagtttgtc ttctcaagat gccaaaaaag
    50101 caggcagatt aattcaaaaa ggtttcatag aagttcaata tttagccaaa gataagaaaa
    50161 caataaaaac agaaaagatt tacaaaatca acagaactct gttagaagag agtcaaatag
    50221 cagcaagagc gaaaaagaga ctggagttga aagaattcct gttagaaaat cctcaaccag
    50281 ggcgcctcac agctttaaac aaacaatttt catctcctgt agttaatttt ttcagagaag
    50341 aaggaatcat tgaggtcatt gaaaaagaag ctagtagatc tgataattat tttaaaggaa
    50401 tcctaaaaac ggattttttg gatttaaatc aggaacaagc taaagtagtt aaaatagttg
    50461 ttgaccgaat tggtaaggat cagaataaac ccttcttgtt ggaggggatt acagggtctg
    50521 gtaagacaga ggtatatctt catgtcattg ataacgtttt aaagttggga aaaacagcga
    50581 ttgttctcgt tccagaaatt tctttaactc cccagatgac gaatcgtttt atttcccgtt
    50641 ttggaaagcg agttgctatt atgcactcgg gcttgtcaga tggtgaaaaa tttgatgagt
    50701 ggcgaaagat aaaatcaggt caagctaaag ttgtcgttgg agctcgttca gcaatttttg
    50761 ctcctttgga aaatatcgga gccatcatta tagatgaaga acatgaatca acttataagc
    50821 aagaatctaa cccaaggtat catgcgcgcg atgtggcctt gctacgtgca gaatatcata
    50881 aagctgtgtt actaatggga tcagcaactc caagtatcga aagccgagct agagctagtc
    50941 gggatgttta taaattttta gaattaaaac atcgtgctaa tcctaaagca cgtattcctc
    51001 aggttgaaat tattgatttt agaaatttta ttggtcaaca agaagttagt aatttcacac
    51061 catatttact agataaaatc cgagatagat tagataagaa agagcaagtg gttcttatgc
    51121 tcaatcgtag aggttattct agttttatta tgtgtcgaaa ctgcggctac gttgatcaat
    51181 gtcctaattg tgacatttct ctgactttac atatggctac aaagaccatg aattgtcatt
    51241 attgtgggtt tgaaaagcct attccaagaa catgtccaaa ttgcaatagt aaaagtatct
    51301 cttactatgg tactggaacc caaaaagctt atgaagaact tttgaaagtt atcccagacg
    51361 caaagatttt acggatggat gttgatacga caaggcaaaa aggcggtcat gagtctattt
    51421 tgaagcgttt cggaaatcat gaagctgata ttttattggg aacacagatg attgctaagg
    51481 gattggattt tccaaatgtt actttagttg gagtactaaa tgcagatact tctctgaatt
    51541 tacctgattt tcgatcatca gagcgaactt ttcagctatt aactcaagtg gctggacgtg
    51601 ctgggcgtgc tgaaaaagaa ggagaggttg tcattcaaac ttataatcct aatcattatg
    51661 ctattcaatt agcccaaaag caagattttg aagcttttta tcaatatgaa atgaatatcc
    51721 gtcgacaact tggttatcct ccttattatt ttacagttgg tttaacatta tcgcataaag
    51781 atgaagagtg gttaatacga aaatcttatg aagtattaag tttattgaag caaggcttta
    51841 gtgataaagt aaagttgtta ggtccaacgc caaaaccaat tgcaagaaca cataaccttt
    51901 atcactacca aattattatc aagtatcgat ttgaggacaa ccttgaactt gttttgaatc
    51961 gcttattaga catcactcaa gacaaggaga atagagatct tcgcttagca atagaccacg
    52021 aaccgcaaaa tatgatgtag aatagtgcta tgatataatt gagaaaagaa agacgaggaa
    52081 aaagcaatga caaaattatt attcatgggt acccctgatt tttcagcgac ggttttaaaa
    52141 ggtattttag cagatggtaa gtatgatgtt ttagctgttg tgacgcaacc agatcgtgct
    52201 gtaggtcgta aaaaagaaat taaaatgacc cctgtaaaag aagtggcctt ggaaaacaac
    52261 attcctgtgt atcagccgga aaaactatct ggaagtccag aattagagca actaatgacc
    52321 ctaggtgcag atggtattgt aacagctgct tttggtcaat tcttaccaac aaagcttttg
    52381 gaatcggtag gttttgcaat taatgtacat gcttctctat taccaaaata tcgtggtggg
    52441 gcaccaattc attatgccat tattaatggc gaaaaagaag ctggggttac catcatggag
    52501 atggttgcaa agatggatgc aggggatatg gtatcaaaag cttccgttga gataactgat
    52561 gaagacaatg tagggacaat gtttgataga ctcgccgttg ttggtcgtga ccttttatta
    52621 gatacgcttc caggttatct atcaggtgat attaagccga taccacaaaa tgaagaagag
    52681 gtaagttttt ctcctaatat tagtccagat gaagagagaa ttgattggaa taaatcatcg
    52741 cgcgacattt ttaaccatgt tcgagggatg tatccatggc cagttgcaca tacccttttg
    52801 gagggaaatc gttttaaact ttatgaagta acaatgtcag aaggaaaggg ctcaccgggt
    52861 caagtgatcg ctaagacaaa gaactcattg acagtagcaa ctggtgacgg agctattgaa
    52921 ctcaaatcag ttcaaccagc agggaaacct cgtatggata taaaagactt tttaaatggt
    52981 gttggtcgta atttagaaat aggagataaa tttggcgaat gattggaaaa agagtgcgcg
    53041 tggtttagct ttaatgactt tagaagaagt ttttgataaa ggagcttatt ctaatatcgc
    53101 acttaataag agtttaaaaa aatctagact atcagataaa gatcgtgcct tagtaacaga
    53161 aattgtctat ggaactgtgg ctagaaaaat aacgctagaa tggtatctat cacactttat
    53221 agttgatcgt gataagcttg aattatgggt atatcatctc ttgctactga gtctttatca
    53281 actactctat ctggacaaca tcccagatca cgctattgtt aatgatgctg tcactattgc
    53341 taagaatcga ggcaataaaa agggtgctga aaagttaatt aatgccgttt tacgaagagt
    53401 aagttcggag acgctccctg aaattgcatc aataaaacgc caaaacaaac gttactctgt
    53461 tgcttattcc atgccagtgt ggctcgtaaa aaaattaatt gaccaatatg gtgagacaag
    53521 agctttagca attatggaga gtttatttga gcgaaataaa gccagtcttc gtgtgactga
    53581 cttatcgcaa aaacaaacta ttaaagaaac tttaaatgtt agagattcac atattgcaga
    53641 gacagcttta gttgcagact caggaaattt tgcatcgaca tcatttttcc aagatggcct
    53701 cattactatc caagatgaat ctagtcaatt agttgctcca acactcaaag tctcgggtaa
    53761 tgaccaagta ttggatgcat gtagtgcacc aggaggaaaa acaagtcata tcgcctcata
    53821 tttgactaca ggggcagtaa ctgctttaga tttgtatgat cataaattgg aattggttat
    53881 ggaaaatgca aaacgcctag gtctgtcaga taagataaaa actaaaaagt tagatgcaag
    53941 taaagctcat gagtattttc ctgaggatac ttttgacaaa attttagtag acgcaccttg
    54001 ttcaggtatc gggctaattc gccgtaaacc agatattaag tacaataaag ctaatcaaga
    54061 ctttgaagcc ctccaagaga ttcaattgtc tatcctatct agcgtttgtc aaacgctacg
    54121 taaaggtggt ataataacct atagcacttg taccattttt gaggaagaaa attttcaagt
    54181 tattgaaaaa ttcttagaaa accaccccaa ttttgagcaa gtagaactga gtcatacaca
    54241 agaggatatt gtaaagagag gctgtattag tataagtcct gaacaatatc atacagatgg
    54301 cttttttatt ggacaagtca aacgtatctt gtagtgtcta ggaggaaact gacatcgtca
    54361 gaaaaaaata tatggaaata tctcttttaa cagatattgg gcaacgtcgt tccaataatc
    54421 aagattttat taatcaattt gaaaataaag ccggcgtccc actaatcatc ttagcagatg
    54481 gtatgggagg acatcgtgca ggaaatattg ctagcgagat gactgtaact gatctaggaa
    54541 gtgattgggc ggagacagat ttttctgagt tatcagaaat tcgtgattgg atgcttgtct
    54601 ctattgaaac tgagaatcgc aaaatatatg aattaggtca gtcagatgat tacaaaggta
    54661 tgggaactac cattgaagca gttgccattg taggagataa tattattttc gcacatgtag
    54721 gagattcacg tatcggtatc gttcgtcaag gggagtatca tttattgacg agtgatcatt
    54781 ctcttgttaa tgaattggta aaagccggcc aattaacaga agaagaagct gctagtcatc
    54841 ctcaaaaaaa tattattacg caatcaattg gtcaagccaa tccagtcgaa cctgatttag
    54901 gagttcatct acttgaagaa ggtgactacc ttgtagttaa tagtgatgga ctaactaata
    54961 tgttgagtaa tgccgatatt gctacagtac taacacaaga aaaaacctta gatgataaga
    55021 atcaagattt aatcactcta gcaaaccata gaggaggtct tgataatatc actgtggcac
    55081 tagtttacgt tgaaagtgag gcggtttaat gattcagatt ggcaaattat ttgctggtcg
    55141 ttatcggatc cttaaatcaa tcggacgagg tggaatggcg gatgtttatc tagctcgaga
    55201 tctaatttta gataatgaag aggtcgcaat aaaagtattg cgaaccaatt atcaaacaga
    55261 tcagattgcg gttgctcgct tccagagaga agcacgagca atggctgagc tgacacatcc
    55321 taatattgtc gctattcgtg atataggtga agaagacgga cagcagttct tagtaatgga
    55381 atatgttgat ggattcgatt taaaaaagta tattcaagac aatgcacccc tatcaaataa
    55441 tgaagttgtt cgaatcatga atgaagtcct ttcagccatg tcactggcac atcagaaagg
    55501 tatcgtacac cgtgatttaa aacctcaaaa tattttatta actaaaaaag ggactgtaaa
    55561 agttacagat ttcggtattg cggtagcgtt tgctgaaact agtttaacgc aaacgaattc
    55621 tatgttaggt agtgttcatt atttatcgcc agagcaggcg cgtggttcaa aagcgactgt
    55681 tcagagtgat atttatgcca tgggaatcat gctttttgaa atgttaactg gccatattcc
    55741 ttatgatggg gatagtgcag ttactattgc cctacagcac ttccaaaaac cactaccatc
    55801 aatccttgca gaaaataaaa gcgttccaca agctttggaa aacattgtca ttaaagcaac
    55861 agctaaaaag ctaacagatc gctataagac aacttatgag atgagacgtg atttatcaac
    55921 tgctctaagc tcgacacgtc accgtgaacc aaaacttgtt tttaatgata ctgagagtac
    55981 taagacacta ccaaaagtaa catctacagt atcatcttta acgacagaac aactgttgcg
    56041 taatcaaaaa caggccaaaa caacagaaaa aataactcct gattctgcga gtaacgataa
    56101 aacgaagtct aaaaagaagg cttctcatcg acttcttggt acaatcatga aattattctt
    56161 tgcactctgt gttgtaggaa taattgtatt tgcttataaa attttagtaa gtcctacgac
    56221 tattcgtgtt ccagatgtta gcaacaaaac agtagctcaa gcaaaaatga cattggaaaa
    56281 ttctggattg aaagtaggag ctattcgtaa tattgagagt gatagtgttt cagaaggcct
    56341 tgtcgtaaaa acagaccctg ctgcaggacg ttctcgtaga gaaggggcta aagttaatct
    56401 ttatattgcc actccaaaca agagtttcac tctaggtaat tataaggaac ataattacaa
    56461 agatatcctc aaagatcttc aaggcaaagg tgttaaaaaa tctttaatca aggttaagcg
    56521 taaaattaat aatgattata ctactggtac aatcttagca cagtcgcttc cagaaggaac
    56581 atcttttaat cctgatggta ataagaaatt aacacttacg gttgctgtta atgatccaat
    56641 gattatgcct gatgtaacag gtatgacagt tggagaagta atagaaacct tgacagacct
    56701 cggattagat gcagataatc ttgtcttcta tcaaatgcaa aatggcgttt atcaagcagt
    56761 agtaacacct ccatcttcaa gtaagatagc aagtcaggat ccttattacg gaggagaagt
    56821 tggattaagg agaggagata aagttaagct gtatcttctt ggttctaaaa caacaaataa
    56881 ctcatcatct acgccaatag attcttcggc ttcaagctca acaggaacaa ctacctcgga
    56941 ttctgtaagc tcatcaacgg atgctagcac ttctgatagt agtagcactt ctacaagtag
    57001 ttcaacacta ccaagtgata gtacaactaa tacaggaaca gctaacaatc cattaactca
    57061 ataaaattaa aaagaagatg ttaaagtctt ctttttaatt tgctatggta gagaccatgc
    57121 ctagaaaggt tctgccaata tgccaaattt ttctctcaat aatggtgcaa agtatagggc
    57181 tttttgataa aatacgtcat atagagtaat ggaaggatta ttatgaaaaa atttcagttt
    57241 tttctacttg ttgaagccgt tgtcttagtt atgggattaa tgaaaatttt atcagatgac
    57301 tggacaagtt ttatttttat tctcgctttg attcttttag ccttaagatt ttataacaac
    57361 gatagtcgtc ataattttct gttaacgaca agtttgttat tactattttt aatctttatg
    57421 cttaaccctt acattattgc agccgttgtt tttgcagttt tgtatgttct aatcaatcat
    57481 ttctcgcaag ttaagaaaaa aaatcgttat gctttgattc aatttaagaa ccatcaatta
    57541 gatgttaaga caacacgaaa ccaatggtta ggtactgatc agcacgaaag tgacttttat
    57601 gcttttgaag atattaatat tataagaatc tctgggactg atacaattga tttaacaaat
    57661 gttattgtat ctgggcagga taatgttatt ataattcaaa aagtttttgg tgataccaaa
    57721 gtattggttc ctttagacgt tgccgtaaag gctgatatta gttctgttta cggtagtgta
    57781 cagtactttg attttgagga gtatgatcta agaaatgaat ctataaaatt gtcacaagag
    57841 gaagaatatt accttttgaa acgtgtcaaa ttagttgtaa acactatcgc tgggaaagtt
    57901 gaggttagtc gtaaatgaaa aagcaccact actttttagc tttcttctat ggaagtgtta
    57961 ttatatttgc catttgtttt gtcattattg atagtttggg tgttaatttg gtgcacctct
    58021 atcaaacgtc tcgtttgtgg ctgattgaac aactgatttt ctctattttc tttctatctt
    58081 tagcggtaac tatcctttta ttgctaactt ggtttttatt agacgataat agtaaacgtc
    58141 aaattaacca taatttacgt cgcattttaa ataaccaatc aataaatgtt accgatgatg
    58201 gtactgaaat atcgaccaat attcaacggc tctctaaaaa aatgaattta atgacagcaa
    58261 gtctacaatc taaggaaaat agtcgtattt taaaaagcca agaaattgtg aaacaagaac
    58321 gtaagcgtat tgcacgtgat ttacacgaca cagttagtca ggatttgttc gcagcttcca
    58381 tggttttgtc agggattgca cagaatgttt ctcagctaga cgttgatcag gttggctcgc
    58441 aattattagc agtagaggaa atgcttcagc atgcacaaaa tgatttgaga attctgctac
    58501 ttcatttgag gccagttgaa ttagaaaata agaccctatc tgaaggattc aaaatgatat
    58561 tgaaagaatt gaccgataag agcgacattg aagttgttta tcacgaatca attctaacct
    58621 tacccaaaaa gattgaagac aatattttta gaattggtca agaatttatc agtaatactt
    58681 taaagcattc acaagccagt cgtctcgaag tgtacctcaa tcaaacagag aatgaattgc
    58741 agttaaaaat gattgataat ggtattggat ttgatatgga ttctgtctat gatttaagtt
    58801 atggattaaa aaatattgaa gatcgagtag aagatttagc aggaaattta cagcttctca
    58861 gtcagcctgg taaaggagtt gctatggata taagattacc tttagtaaat cagagtgagg
    58921 ataaaaatgg ataaaataaa aattgtactt gttgatgacc atgagatggt tcgtctcggt
    58981 ttaaaaagtt ttttaaactt acaagctgat gttgaagtaa taggtgaagc ctcaaatggt
    59041 ttagaaggca taaaaaaagc actggagtta cgtccagacg ttgttgttat ggatttagta
    59101 atgcctgaaa tggacggtgt agaagcaacc ttagcattgt tgaaggattg gccagaagct
    59161 gctattttag tcttaacatc ttacttggac aatgaaaaaa tttaccctgt tattgaagca
    59221 ggtgccaaag gctatatgtt aaagacctca agtgcagcag agattcttaa tgccattcgt
    59281 aaagttgcaa ggggagaaca agctattgaa aatgaagtag ataagaaaat taaggctcat
    59341 gataaatgtc cagctttaca tgagggctta acagcaaggg aacgtgatat ccttaattta
    59401 ttagccaaag gttatgacaa tcaaaggatt gctgatgaat tatttatctc gttaaagact
    59461 gttaaaactc atgtttctaa tattttggga aaactaaatg tggcagatcg aactcaagcg
    59521 gttgtctatg ctttccaaca ccatttggta ccacaagatg atgaataagt gatataatag
    59581 agacactatg ttaatgaaag tggttagtca tggacgcaaa aactaaatat aaagcaaaaa
    59641 aaataaaggc ggtgtttttt gatattgatg acacgcttcg tgttaaagat acaggttata
    59701 tgccaccatc gatcttaaaa gtttttaaag ctttgaaaga taaaggaatt gttgtaggta
    59761 ttgcttcagg aagggcacga tatggcgttc caaaagaagt tcaagattta aatgccgatt
    59821 attgtgtaaa attaaatgga gcttatgtta aggacaagga taagaatatc atcttccatc
    59881 gtcctattcc tgctgaatat gtagagcaat ataagaaatg ggcagacact gttggtatta
    59941 agtacggttt agctggtcgt catgaggcag ttttatcaga tcgtgatgat ttggtaaatg
    60001 atgcgattga tattgtgtat tctgatctcg aagttaatcc agatttcaat aaagaacatg
    60061 atatttatca gatgtggacg tttgaagata aaggagatag ccttcatcta cctgaaccgt
    60121 tagatgaaca tcttcgcttg atacgttggc atgatcattc ttcagatgtt gtgttaaaag
    60181 gaacatcgaa ggctttaggt gtttctaaag ttgttgaaca tttgggctta aagccagaga
    60241 atatcttggt tttcggagat gagctcaatg atttagaact ctttgattat gctggactag
    60301 ctgtggcgat gggagtgtca cacccagaag ctcaaaaaaa agcagatttt atcacaaaaa
    60361 aagtagaaga agatggcatt ttgtatgccc tggaggaact aggtttgata gaaaaagaat
    60421 taacatttcc acaagtagat attgaaaata cagaaggtcc tgtggctgtc attaaaacaa
    60481 atcacgggga tatgactgta aaactcttcc cggatcatgc tcctaaaacg gtagcaaact
    60541 ttattggttt agctaaacaa ggttattatg atggtattat cttccatcgt attattccag
    60601 attttatgat tcagggtggt gatccaactg gtacaggtat gggtggtgaa tctatctatg
    60661 gggaaagttt tgaagacgaa ttttccgaag aattatataa tgttcgcgga gctctttcaa
    60721 tggctaatgc tggaccaaat acgaatggca gccaattttt catcgtccaa aatacaaaaa
    60781 ttccttatgc gaaaaaggaa ttagagcgag gtggttggcc aacaccaatt gctgagttgt
    60841 atgctggaca aggtggtaca ccgcatctag atcgtcgcca cagtgtattt ggacaattag
    60901 taaaccaaag ttcttttgag gtacttgatg agattgcggc agtagagact ggttctcaag
    60961 acaaaccatt ggaagatgtt gttattttaa cgattgaggt taaagaataa aatgaagatt
    61021 ggcgataagt taaagggaac agtcactggc attaggcctt atggagcctt tgtttctctt
    61081 gaggatggta ggactggact tatccatatt tctgaaatta aaacaggtta cattgataat
    61141 atttatgatg ttcttagtgt aggtgatgaa gtctatgttc aggttataga tgtagatgaa
    61201 tttacacaaa aagcaagttt atcgcttcgg acccttgaag aagaaagaca tcatattcag
    61261 catcgtcatc gattttctaa caatcgtctt aagataggtt tcaaaccact tgaggaaaat
    61321 ttacctagtt gggttgagga aggtctagcc tacttgaata ataaataaac aaaaacctag
    61381 atttgattaa tgacaagtct aggttttaaa tatttaaaag tagcagtgaa tattatactt
    61441 gctaaaaata gcttgataat cgagtagatc ctctgggtgt agtgcagcga tctcttccat
    61501 ttcatattga cgattaagta atcgatattt gttttgtcca aattggtgga aaggtaagag
    61561 ttgcacttga cggatatcta ggcgatcaaa taaacaagca aattcttcag catcttctaa
    61621 tgaatcgtta aaattaggta tgacaggaat acgtagcaca atcgttttac cgttggcaaa
    61681 agcataatga atgtttttga tgattgaagc attcttaacc atcgtctttt cttggtgttt
    61741 gaggctattg taatgtttta aatcggtgta aataaaatca acgtattgga taaggtctat
    61801 gaattgttca tgtctagtat aagcagttgt ttcaatagcc gtgtggattc ccagtgattt
    61861 agcacgtttt aaaattgcct tggcaaattc aaattgtgca aatatttcac cgccagatag
    61921 cgttatgccc ccaccagatt cttcgtaaaa atcaatatct tttaaaacct cttcaataat
    61981 atcatcaacc gatttttctt cgcccacgat gacgctttca ttagtaatgg catctctcat
    62041 tgtttctgga accatttttt gcgactcggg attagcacac caaggacatc taagaggaca
    62101 accctttaag aaaacggttg ttcgaatacc tggaccatcg tgaatactaa aatgttgaat
    62161 gttgaacaca ataccttttt catttgtcat attattttcc tcaatttcat tttaagtata
    62221 ccttttatac gtaacaaagt caattacgaa tgaaagttta ttgaacttgt ttaataaata
    62281 aaaaatgata aaatagaaag gaggtgtttc atgaatagat tagaaaatat tattagttta
    62341 gtatcccagt accaaaaaat agatgttaat acactctcgg agttgttgca agtttctaaa
    62401 gtaaccatac gtaaagattt agataaacta gaaggcaaag ggcttttgca tagagagcac
    62461 ggttatgcag ttttaaacag tggggatgat cttaatgtta ggttatcttt taaccataaa
    62521 actaaaaaag aaattgctgc cttagctgca aatatggtgt ctgacaatga cactatctta
    62581 atagaatcag gctctacttg tgctctttta gcggaaaata tctgtcagac aaagcgaaat
    62641 gtcactattt tgaccaactc ttgctttatt gctaactatt tacgagagta tgattcatgc
    62701 caaattgtct tacttggtgg tgagtatcaa tcaagttcac aggttacggt aggtcctctc
    62761 ttgaaaaaga tgatttcact ttttcatgtc tcgttagctt ttgttgggac tgatggtttt
    62821 gatccaaaac taggatttat gggtagagat ttaatgcgtt ctgaagttgc acaagagatg
    62881 gcgaatgctg ccgatgaagt cattatttta acagattcta gtaagtttaa tcagactgca
    62941 ttggtggaac agcttccgtt gtcaactgta tcacaagtta ttacagataa acatccaaat
    63001 tcggatattg ctaacttgtt ccaagaagca gagattacca ttcaatcttt attacaagta
    63061 ccataaaaga ggctattttt cttacatttt gcacatttgt ggcgataata gataaagtgt
    63121 catgtcaaat tgcacagatg taaccttttt gttaaactga agcaaatgct gaaaggttat
    63181 gagtatgaaa ttagagcgtc aaaaattgtt agctaaagta gcttaccttt actatatgga
    63241 agggaaaagc caatctgaga ttgccaatga attaggaatt tatcgtacaa caataagtcg
    63301 tatgttagcc aaagcgagag aagaaggtct tgttagaatc gaaatttcag attttaatcc
    63361 agaaattttt cagttagaat cctattttaa aagtaagtat caccttaaag atattgagat
    63421 tgtttcttcg cgaaaagata gcgatacttc agagatagag aaggatttag cacatgtagc
    63481 tgcagctatg attcgaaaaa aaattaaaga gaatgacaag gtggggattg cttgggggag
    63541 gacacttagt aaagtagttg aagcaatgcg tcctcatccg gtttctcaag tttcttttgt
    63601 tcctttggcc ggaggtccaa gtcatattaa tgcacgctat cacgttaata ctctggttta
    63661 cgaaatgtca aggcgtttcc aaggaagttg tactttcatt aatgcaactc tagttcaaga
    63721 aaacgctaat ttagcgaaag gaatattaac ttcaaaatat tttgaggggc taatggataa
    63781 ttgggaaaaa cttgatgtgg caattgtggg cgttggtggt aaacctaaaa gcaatgaaca
    63841 acagtggctt gatcttttaa atcaagatga ttttcaatgc ttggatgaag aagcagctgt
    63901 aggtgaaatt acttgtcgtt tttttaatca ttcaggtgat tctgtgaatc aacatttagc
    63961 taaaaggaca ataggaatta ccttagaaca actacagaaa gttcctaata gaatagcggt
    64021 tgctcatggc aattataagg ctgcggcact cttagccgtt ttaaaaaagg ggtatattaa
    64081 tcatttagtt actgattttt ccacagcctt aaatatttta agattagata aagacacctt
    64141 tgtggatact atgtatcaaa agagctagga tttattccta gctcttttaa taatgtttct
    64201 attaccaagt gaattgttat agaataagca caaaagtgcg ctattatcat aaagggcaca
    64261 aggtctattg acgacgatta agggacatgt tacatttgtt ttgcgaaaga aaattagaac
    64321 cttttgtaag aaagtttaaa cttataaaaa atcattaaag cggaggtgct tatggaaatc
    64381 atagtcgcag accaaattat tatggggctc attttgaatg ctggggatgc taaacaacat
    64441 atctatcaag ccttaaaact tgcaaaagaa ggaaattttg cagaaagtaa aattgaaata
    64501 gaactagcag atagtgcact cctagaagct cataatcttc aaacacaatt ccttgcacaa
    64561 gaagcaggag ggacaaggac agatatctca gcactcttta ttcattctca ggatcatttg
    64621 atgacaagta ttactgagat taacttgatt aaagaaatta ttgatttacg ccaggaatta
    64681 catattcaaa aataagtaaa ggggatcaaa tatgataaaa attggtttat tctgtgcagc
    64741 aggtttttca acaggtatgt tagtaaacaa tatgaaaatc gcagcggata aagaagggat
    64801 agaggcacat attgaagctt attcacaagg aaaaattgca gattatgcta aagatttaga
    64861 tgttgcatta cttggtccac aagttagcta tactctagat aaatcaaaat caatctgtga
    64921 tgaatacggt gtaccgatag cagttattcc aatggcagat tatggtatgt tggatggtgt
    64981 aaaagttttg aaacttgctt tgagtttatt ggaaacaaga aaagaggctt gatatgtcaa
    65041 agtttgatag tcagaaaata attactccga ttatgaagtt tgtcaatatg cgagggatta
    65101 ttgcactcaa agatggcatg ctagcaattt taccactaac agttgttggg agtctctttt
    65161 taatattagg gcagcttcca tttaaaggtc ttaatcaagc catagctaat gtttttggac
    65221 cagaatggac agaaccattt atgcaagttt attcaggaac ttttgcgatt atgggcttga
    65281 tttcctgttt tgcaattgct tatgcttatg ctaaaaatag tggtgtagaa ccgttacctg
    65341 ctggcgtttt atcactttcg tctttcttta ttttaatgaa atcatcttat attcctgtaa
    65401 aaggagaagc aatagcggac gctatttcta aggtttggtt tggcggtcag gggattattg
    65461 gagcaattat tattggctta gtagttggtg ctatctatac atggtttatc caacatcata
    65521 ttgttataaa aatgccagag caagtaccac aggcaatagc aaaacaattt gaagctatga
    65581 ttccagcttt tgttatcttt cttttatcga tgattgttta tttgattgct aaggtaacaa
    65641 ctggaggtac ctttattgag atgatttatg atatcattca agtaccgttg caaggcttaa
    65701 caggctcact ttatggagca attgggattg ctttctttat ttcattctta tggtggtttg
    65761 gtgtccatgg ccaatctgtg gtgaatggta ttgtgactgc tttgttacta tccaatttag
    65821 atgccaataa gtctttatta gcagcaaatc gcttaacatt agataatggc gctcacattg
    65881 taacacagca gtttttggat agtttcttga ttttatcggg ctcaggaata acttttggac
    65941 tagttattgc gatgcttttt gcagcaaaat caaaacaata caaggcactt gggaaagtgg
    66001 ccgcttttcc cgcaattttc aatgttaacg agccaatcgt ctttggcttt ccaatcgtaa
    66061 tgaatcctgt gatgtttctg ccttttattt tagtaccagt tttggcagct ttaattgttt
    66121 atggagctat tgcggttggt tttatgcaac cattctcagg tgttacttta ccatggagta
    66181 ctccagcaat tatttctgga ttcatggtag gtggttggca aggtgcactc gtccaaatag
    66241 tcattttagc tatctcaact gctgtttact tcccattctt taaaatccaa gataatatta
    66301 cttacaaaaa tgaatgtgaa atggaaaggg gatagctagc agataagagg ttgaggtgtg
    66361 taatgcacct taaccttttt ctaaaaatgc acatttgtgc aactttagat gtagcattct
    66421 attctttatt ttcttcttgt tattttagga tttaggtgct attatattta cgaagaaaaa
    66481 ctttttagtt tcgaaagaaa caagcggagg ctgttatgac caaacaatta ctcactcaaa
    66541 aatatacaaa tgatgttcaa caaaattctc aaaagcattt tggctatttg acagagcgca
    66601 tgtattctta tcgcgataag gttcttgata agaaaccatt catagatgca gaacgtgcta
    66661 tcttggtaac tgaagcctat caaaagcatc aggaaaaacc taatgtttta aagagagctt
    66721 acatgttgca aaatattttg gaaaaaatga caatctatat tgacgatgag acaatgattg
    66781 tcggtaacca agcttcatct gataaagatg ctcctatttt tccagaatac actctagaat
    66841 ttgttgtaaa tgaactagac ctttttgaaa aacgagatgg tgacgtcttt tacattacag
    66901 aagaaaccaa agaacaaatt agaaatattg cacctttctg ggaaaataat aatttacggg
    66961 ctcgtgcggg cgttatgctt ccagaagaag ttcaagttta tatggagaca ggcttctttg
    67021 ggatggaagg taagatgaat tctggggatg ctcacttagc tgttaactat cagaagttac
    67081 tagaagaagg gcttattggc tttgaaaaaa aagcaagaaa agctaaagct gacttagatt
    67141 taacaaagcc tgagagtatt gacaaatatc atttctatga ttctatttta attacaatag
    67201 aagctgttaa aacctatgca gagcgttttg ctatcctagc aaaaaaacaa gctaaaacag
    67261 caaatactaa gagacgtcag gaattgttag atatcgctag tatttgtgaa cgagtgccat
    67321 attacccagc tgaaacgttt gcagaagctg ttcaatcggt ttggtttatt cagtgtattc
    67381 ttcaaataga atcaaacggt cattctctat cttatggacg ctttgatcaa tacatgtatc
    67441 catatgttaa agctgactta gaagctggac gtgagactga ggattcgatc gttgagcgcc
    67501 taactaacct atggattaaa acaataacta ttaataaggt tcgtagccaa gctcatacgt
    67561 tttcgtctgc tggtagtcct ctttaccaaa atgtaacgat tggaggacaa acccgacata
    67621 aggaagatgc tgttaatcct ttatcatttc tagtactaaa atcagtcgct cagacacact
    67681 taccacagcc taatctaacg gttcgttatc atgctaacct agacaagtca ttcatgaacg
    67741 aagctattga agttatgaaa ttaggttttg gtatgccagc ttttaataat gatgaaatta
    67801 ttattccatc atttattaaa aaaggagttt ccgaagaaga tgcctatgat tattcagcga
    67861 ttggttgtgt tgaaacagca gttcctggga aatggggtta tcgttgtacc ggcatgagct
    67921 atatcaactt ccctaaagtg ttattaatta caatgaataa tggcattgac ccggcttctg
    67981 ggaagagatt tgcgccttct tatgggcatt tcacccagat gacctcatat aaagaactta
    68041 aagaagcttg ggacaaaaca cttcgttact taactcgaat gagtgttatt gttgaaaatg
    68101 ctattgatat tagtctcgaa agagaagtac ctgatattct ttgttcagct ttaactgatg
    68161 attgtattgg acgaggtaag catttaaaag aaggtggtgc agtatatgac tacatatctg
    68221 gtctacaagt tggaatcgct aatctttcag actcacttgc tgcgcttaaa aaattagtat
    68281 ttgaagaaaa acgcctaacc acattagaag tatggcaagc attgcagagt gattacgcag
    68341 gtccgcgtgg tgaagaaatt cgtcaaatgt tgatcaatga agcaccaaaa tatggaaatg
    68401 atgatgacta cgcggatagt ttagtacgtg agtgttatga tgtttatgtt gaagaaattg
    68461 ctaaatatcc caacacacgc tatggacgtg gaccaatcgg tggtattcgc tattcaggaa
    68521 cgtcctctat ttcagcaaat gtaggtcaag gacgaggtac tttagcaact ccagatggta
    68581 gacatgcagg aactccatta gctgaaggtt gttcaccatc tcataatatg gacaaaaagg
    68641 gacctacctc tgttttgaaa tcagtttcga agttaccaac tgatgagatt gtcggcggtg
    68701 tactccttaa ccaaaaagtc aaccctcaaa ccttggcaaa agaagaagat aaacaaaaat
    68761 taattgcctt acttagaaca ttcttcaatc gccttcatgg ttaccatatc cagtataatg
    68821 ttgtctcacg tgagactttg attgatgctc agaagcaccc tgaaaaacac cgcgatttga
    68881 ttgtgcgtgt agcaggttat tctgcattct ttaatgtcct atctaaggca actcaagatg
    68941 atattattgc acgtacagaa catgctcttt aggagattat tatggaattt ttacttgaca
    69001 ctttaaattt agaagctata aaaaaatggc atcatatact gccactagca ggtgtaactt
    69061 caaatcccac cattgctaag aaagaaggag atatacattt tttccaacgc attcgtgatg
    69121 ttcgagaaat tattggatgt gaagcaagtt tgcatgttca agtagtggcc aaagattatc
    69181 aagggatact ggatgatgct gctaaaatac gccaagaaac ggatgatgac atttatatca
    69241 aggttccagt tacgccagat ggattagcag caattaaaac gttaaaagca gaaggttata
    69301 atattacagc cacagcaata tacaccagta tgcaaggtct tttggctata tcagctggag
    69361 cagactacct agcaccttac tttaaccgaa tggagaacct tgatattgat gcgacacaag
    69421 ttattaagga attagcgcaa gcaatagaga ggacaggttc ttctagcaaa attttagcag
    69481 ctagttttaa aaacgcttca caagtaacga aggccttatc acagggtgct caatcaatca
    69541 ccgcgggacc agatattttt gaatcagtct ttgcgatgcc atctattgca aaagctgtta
    69601 acgactttgc agatgattgg aaagccagtc aacactcaga acatatttaa cagagaacgc
    69661 caacttttga ggctgtatct ttaacataaa attttttaaa gaagaaagag gttgagaatg
    69721 aaagttttcg caagtccatc gcgctatata caaggaaagg atgctttatt ccaaagtatt
    69781 gaacatatca aatctttagg tcagactcca cttatccttt gtgatgatgt tgtttataat
    69841 atcgttggag aaaggttttt atcttatcta caagacgatt tgttaccaca tcgtgtctcc
    69901 tttaacggag aagcatctga taatgaaatc aatcgtgtcg ttgctgttgc aaaagaaaag
    69961 aatagtgacc ttattattgg acttgggggt ggaaaaacta ttgactctgc aaaggcaatt
    70021 gcagataaag ttaatttacc agtgatcatt gcaccaacag tagcttcaac agatgcccca
    70081 acatcagcgt tatcagtaat ctatacagat gaaggagctt ttgaaaaata tattttttat
    70141 tcaaagaatc cagacttagt tttggtcgat actcaagtca ttgctcaagc gccaaaacgt
    70201 ttactagctt ctgggattgc agatggctta gctacttggg ttgaagcgag agcagtctta
    70261 caaaaaaatg ggatagcgat ggctggcggt agacagactt tagcgggcgt ggcaattgct
    70321 caagcatgtg agcgtacttt atttaatgat agtttacaag ctttagcggc ttgtgatgtc
    70381 aaagtggtta caaaagcttt agaaaatgtt attgaagcta ataccttact tagtggttta
    70441 ggttttgaaa gcgcaggatt agctgcagct catgctattc ataatggttt tactgccttg
    70501 agtggagata ttcatcactt aacccatggt gaaaaagttg cctatggaac gttgacacaa
    70561 cttttcctcg aaaatagacc aaaggaagaa attgatcgct acattaatct ttaccaagcg
    70621 attggaatgc caactacact tgcagaactc catttgggtg atgcaacata tgaagaactt
    70681 ttaaaagttg gtcagcaagc aacaattgaa ggtgaaacaa tacatgaaat gccattcaaa
    70741 attagtgcag aggatgtcgc tgcagccctg ttagcagttg accgctatgt ttcaaatcat
    70801 caataaaaac aatagaacca agtgaaattg cttggttcta tttgtcgttt taatatttta
    70861 actaaatagg gcttactagt atttaaagag atataaacta aaaaagatca gacaacatat
    70921 ctaatctttt ttagttatcg tttactgaag attaaagtta ttcaaaatca tacaggcttg
    70981 ttgataagta acgttcgcca ttatcaggga gaagagctaa aacctttttc ccttttccta
    71041 gttgtttggc cacttcaata gcagcataaa tagcagctga agcagaaatt cctgcgagga
    71101 aaccttctag tccgccgatg atacgtcctg tttcaatagc atcgttagat gtaacgcgaa
    71161 tgatatgatc ataagaatct gtagccaatg tttctggaat gaaacctgca gaaatccctt
    71221 gtattttatg aggcccaggt tgctcaccag aaaggattgc agattcatca gcttctacag
    71281 cataaatagc gacatcagga ttgacttttt ttaatgcacg agagacacca gtaattgtcc
    71341 caccagttcc cacacctgcg ataaaagcat ctaaaccttt ttcaccaaaa gtctcgataa
    71401 tttccctacc agtcgttttt tcgtggattt ctggattggc ttgattatta aattgcaggg
    71461 gtagccaagc attttgctct gcagaaattt cttttgcctt agcaatagct cccttcatcc
    71521 cttcactacc aggtgttagt actaattgag caccataggc ttgaatgatt ttacgacgtt
    71581 caattgacat ggtttctggc ataacaataa taacattata ccctttagct tttcctaccc
    71641 aagccaaacc gatacctgta ttaccagatg taggttctac gattgtatct ccggctttaa
    71701 gaatcccttt ttgctctgca tcttcaatca tagctagagc gatacgatct ttgacagatg
    71761 aacctgggtt aaaggattct aacttaacgt acacctctgc agctccttct ggaacaatat
    71821 gatgtaattg aataatgggt gtatttccga ttaagtctgt aatagaatta taaatttttg
    71881 acataagcta tacctcttca tgacattggt taagaatatt ataaaactca cattgattaa
    71941 agtaaaatat caattcttta tcaagtgata gctattattt atcggtttca gccttaaagg
    72001 gaatttcaac aatttgactc ccttcttcag ttaacaaagc ttttccttgg tagaactctg
    72061 tcaaattagt tattgtgtgt tcaaattcct tagggtcaac gtatatttta caggttacag
    72121 cttctaaaaa ttctgtttca aactcctgaa ggtggtgctc ttttaggaag ttgtcaaaag
    72181 tttgatattg aggataagtt aattgaatac gaataccaat ttgttcttta acttctacta
    72241 ccccgatctc tttgatggca ttagctacgc taccagagta ggcacgaata agcccccctg
    72301 tgcctaattt gatacctcca aaataccgtg taacgacggc aacaacattt gtcaggcctt
    72361 gcttttcaag tacggttagc atagggatac ctgctgtacc actaggttca ccatcatcac
    72421 tagatcgttt tatatcccct ttttcgccaa taaccatagc tgaacacgaa tgattggctt
    72481 tgtagtgcgc tttcttgatt tgcgtaatat aattacgccc ctcctcttcg gactctactc
    72541 gctttagatg acaaataaat cgcgattttt taatttcttc ttcgacaatt ccgtctgatt
    72601 ttatcgtttt atagttcata gagaaatttt acaaaaattc ttcaaattta gcaaaaaaat
    72661 ccgaatgaaa taatatggaa aattatttag gtagactttg gacaaaagca caattatctg
    72721 agcaacttag aaaaattgca attaacttgc catcttttat aaagaaagga agcgactata
    72781 tttgtactcg atgctctagt tctgttgcca aaaattgcca attaccaact ggaaattatt
    72841 attgtagaga atgtatagtt tttggaagag ttactagtaa tgaaaattta tattatttcc
    72901 cacaaaaaac attttctaaa acgaattctt taaaatggaa agggggactg acaccttatc
    72961 aaaatgaggt ttcagaagag ttgttgaaag gtatttcaag taaggaaaat cttcttgttc
    73021 atgctgtgac aggggctggt aaaacagaaa tgatatacca ctctatcgcc aaagtaattg
    73081 atactggagg gagcgtttgt attgccagtc ctagaattga tgtttgttta gaattgcata
    73141 agcgactttc aaacgatttt agatgtgcca taacattaat gcatggagaa tctccttctt
    73201 atcagagatc ccctttaact attgccacaa cacatcaatt gttaaaattt tatcatgctt
    73261 ttgatttgct aattgttgat gaagttgatg cttttccata tgtcgacaac ccgatactat
    73321 accatggtgt aaaacaggcc ttaaagaaaa acggaacaag tatttttctg actgcaactt
    73381 caacgactga gttagaaaga aaagttgcaa gaaaagaact aaaaaaatta catctagcta
    73441 ggagatttca tgccaatccg ttagtgatac ccgagatggt ttgggtaagt ggtatccaga
    73501 aaagcttgca gacacaaaaa ctacctccca aattatatca gctgataaat aaacagcgac
    73561 aaacgagata tcctttgtta ttatttttcc cccatattgc agaaggtcag gtattcaccg
    73621 aaatgttaag acaagccttt cctatggaaa aaattggttt tgtttcaagt aagagcacat
    73681 ctaggttaaa gttagtgcaa gattttcgag ataacaaatt gtctatccta gtatcaacta
    73741 ccattctaga gagaggagta actttcccta gcgtcgacgt ttttgttata caagcaaatc
    73801 atcatttgtt tacgaaaagt tccttggtac aaatttcagg aagagttgga agagcattag
    73861 aaagacccga agggctcctt tacttccttc atgatgggaa aagtaaatcg atgcaccaag
    73921 ccataaagga gattaaaaac atgaatcata taggaggatt ttaatgactt gtttgttatg
    73981 tcatgaaata gatttatcgc aattaacttt tgtggaactt atacttctaa aaccaaagca
    74041 aaacgtaatt tgtcaaacgt gcaagggtag ttttgaagca cttagtaggg agatgggttg
    74101 ccaaacatgt tgcaagcaaa tcctccgaaa acaatgtcaa gattgtattt attggggtaa
    74161 aaaaggtata gaggtaaatc atttttccct ttatagatac aatgaagcaa tgaaaaaaat
    74221 atttttctct ttttaaattt caaggggatt atttgttgaa agatgttttt acaaaagaaa
    74281 taaaggctgc tctaaaaagg tataagggct acactatagt gccagttccc ttgagtcatg
    74341 aaggatacca aaataggcaa tttaatcaag tgattgcttt tctacaatcg gcaaatatac
    74401 cttataaaaa tattctttct aaaaaagatg gaggtaagca atcggctaat aataaagaag
    74461 aaagactcaa acaagttcag caatttacat taaaaaatga ggctga
//
LOCUS       DATROD010000003        58178 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.003, whole genome shotgun sequence.
ACCESSION   DATROD010000003 DATROD010000000
VERSION     DATROD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 58178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58178
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.003"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(648..1286)
                     /locus_tag="VBC74_000151"
     CDS             complement(648..1286)
                     /locus_tag="VBC74_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEO6941135.1"
                     /translation="MIKSKILLQLMQGKIVAVIRGNSQEEAFQAAQACIKGGISAIEI
                     AYTNSKASQVIEQLVTQYTNQEQVVVGAGTVLDAETARMAILAGAKFIVSPAFNLQTA
                     KLCNRYAIPYLPGCMTLSEITTALEAGCEIIKIFPGGTLGTSFISSLKAPLPQVQIMV
                     TGGVNLTNAKDWFLSGVTAIGIGGEFNKLAALGEFDKITEMAKQYKIQLNLN"
     gene            complement(1298..2305)
                     /locus_tag="VBC74_000152"
     CDS             complement(1298..2305)
                     /locus_tag="VBC74_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEO6941136.1"
                     /translation="MSKILFFGEPLIRITPKENDYFADSISTKLFYGGSEVNIARALQ
                     GFGQDTKLLSALPNNPIGNSFLQFLKAQGIDTHSIQWVGERVGLYFLENSFACRKGEV
                     VYDRDYSSLHDFRINQIDFDQLFEGVSLFHFSGITLSLDESIQEITLLLLKEAKKREI
                     TISLDLNFRSKLISPKNAKILFSQFATFADICFGIEPLMVDSQDTTFFNRDEATIEDI
                     KERMISLINHFDFQVIFHTKRLQDEWGRNHYQAYIANRKQEFVTSKEITTAVNQRIGS
                     GDAFVAGALYQLLQHSDSKTVIDFAVASASLKCALEGDNMFETVTAVNKVLNQSKDII
                     R"
     gene            complement(2331..2969)
                     /gene="dhuI"
                     /locus_tag="VBC74_000153"
     CDS             complement(2331..2969)
                     /gene="dhuI"
                     /locus_tag="VBC74_000153"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase DhuI"
                     /protein_id="HEO6941137.1"
                     /translation="MKIALINENSQASKNTLIYKELKAVSKKKGFDVFNYGMYGKEEE
                     SQLTYVQNGLLTAILLNSGAADFVITGCGTGIGAMLACNSFPGVVCGFAADSVDAYLF
                     SQVNGGNALSLPFAKGFGWGAELNLRYLFERLFEDEKGGGYPKERAVPEQRNARILSE
                     VKQITYRDLLSVLKEIDQDFLKETISGEHFQEYFFANCQNQNIADYLKSVLD"
     gene            complement(2986..3798)
                     /gene="dhuD"
                     /locus_tag="VBC74_000154"
     CDS             complement(2986..3798)
                     /gene="dhuD"
                     /locus_tag="VBC74_000154"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric 2-dehydro-3-deoxy-D-gluconate
                     5-dehydrogenase DhuD"
                     /protein_id="HEO6941138.1"
                     /translation="MTEQFLKDNFSLEGKVALITGASYGIGFSIATAFARAGATIVFN
                     DIKQELVDKGISAYKKLGIKAHGYVCDVTDEDGINEMVNKISQDVGVIDILVNNAGII
                     KRTPMLEMSAADFRQVIDIDLNAPFIVSKAVLPGMIQKGHGKIINICSMMSELGRETV
                     AAYAAAKGGLKMLTKNIASEYGSANIQCNGIGPGYIATPQTAPLRERQDDGSRHPFDQ
                     FIIAKTPAARWGEAEDLGAPAIFLASDASNFINGHILYVDGGILAYIGKQPQ"
     gene            4076..4510
                     /locus_tag="VBC74_000155"
     CDS             4076..4510
                     /locus_tag="VBC74_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6941139.1"
                     /translation="MIKIIIVAHGNFPDGILSSLELIAGHQEYVVGINFIAGMSSNDV
                     RVALQREVIDFKEILVLTDLLGGTPFNVSSALSVEYTDKKIKVLSGLNLSMLMEAVLS
                     RTMFEHVDDLVDKVITSSHEGIVDFSTCLATQTAEATFEGGI"
     gene            4513..5709
                     /locus_tag="VBC74_000156"
     CDS             4513..5709
                     /locus_tag="VBC74_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEO6941140.1"
                     /translation="MMKIKPVKVESIENPKRFLDSRLLTKIEVEEAIEKALKQLYINI
                     DYFGEEYPTPATFNNTYKVMDNTEWTNGFWTGCLWLAYEYNQDKKLKNIAHKNVLSFL
                     NRINNRIALDHHDLGFLYTPSCTAEYRINGDAKALEATIKAADKLMERYQEKGGFIQA
                     WGELGYKEHYRLIIDCLLNIQLLFFAYEQTGDEKYRQVAVNHFYASANNVVRDDSSAF
                     HTFYFDPETGEPLKGVTRQGYSDESSWARGQAWGIYGIPLSYRKMKDYQQIILFKGMT
                     NYFLNRLPEDKVSYWDLIFTDGSGQPRDTSATATAVCGIHEMLKHLPEVDPDKETYKY
                     AMHTMLRSLIEQYSNSEFIAGRPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYK
                     DWELYW"
     gene            5764..6258
                     /locus_tag="VBC74_000157"
     CDS             5764..6258
                     /locus_tag="VBC74_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEO6941141.1"
                     /translation="MAAGPNIVMTRVDERLIHGQGQLWVKFLSCNTVIVANDDVSKDH
                     LQQTLMKTVVPESIALRFFDIQKVIDIIHKANPAQTIFIIVKDLKDVYRLVAGGVPIK
                     EINIGNIHNGEGKEQVSRSIFLGMKDKEIIRKLNQEYHIAFNTKTTPTGNDGAVEVNI
                     LDYI"
     gene            6294..7160
                     /locus_tag="VBC74_000158"
     CDS             6294..7160
                     /locus_tag="VBC74_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO6941142.1"
                     /translation="MDISILQAVLIGLWTAFCFSGMLLGLYTNRCIVLSLGVGVILGD
                     IQTALAVGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITTAGTKGKITPEAALA
                     LSTPIAVGIQFLQTATYTAFAGAPETAKKALQAGNFRGFKIAANGTIWAFAGLGFGLG
                     VLGALSTQTLTDLFALIPPVLLNGLTLAGKMLPAIGFAMILSVMAKKELIPYILLGYV
                     LAVYFGLPVLTPTANGDGVLTSVATNSVLGVPTIGVAIIATIFALLDIFRKPAAPTKE
                     TKTEGDNQDDWI"
     gene            7147..7962
                     /locus_tag="VBC74_000159"
     CDS             7147..7962
                     /locus_tag="VBC74_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO6941143.1"
                     /translation="MTGSKKLAKSDYTKTALRAFYLQNGFNYSNYQGLGYANVIYPAL
                     KKYYGDDKKALAGALEENVEFYNTNLHFLPFVTSLHLAMLDNERPEEEIRGIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLATDGLVMGPILFFVAMNTILTGIKLVTGMYGYR
                     LGTSFIDKLSEQMSVISRAANIVGVTVISSLAATQVKLTIPYTFAPEKVTSTAQKIVT
                     VQGMLDKIAPALLPALYTFLMFYLIKNKKWTTYKLVILTVIIGILGSWLGILA"
     gene            8042..9946
                     /locus_tag="VBC74_000160"
     CDS             8042..9946
                     /locus_tag="VBC74_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEO6941144.1"
                     /translation="MKDRLYNKFKDFDREFCQKYIKTYQSNAYQEMKASVNLMMRNTF
                     VFNDNWDMEPCSKAYCLDPLEWDNPVTDDPEWLYMLNRQTYLFKFLVVYIVEGDKSYL
                     RQMKYFMYHWIDCQFTLKPEGAVSRTIDTGIRCMSWLKVLIFLDYFGLITETKKIKLL
                     TSLREQITYMRDYYREKDSLSNWGILQTTAILACLYYYEDELNLPEIQSFAEEELLLQ
                     IKLQILDDGSQYEQSIMYHVEVLKSLMELVILAPKYYLPLEETIEKMVTYLIAMTGPD
                     YCQLAIGDSDVTDTRDILTLATLVLKSSKTKSFSFDNVNLETLLLFGKPSIYLFEEIP
                     RATIGESAYLFPDSGHVCLRDDRRYIFFKNGPFGSAHTHSDNNSVCLYDKKKPIFIDA
                     GRYTYKEEQLRYDFKRSTSHSTCTLDGQSLEMIKDSWTYNSYPKCDYCQLTSKDRYHL
                     VEGQLHVQRASDIYYHKRWLLTLPQAITLVIDKVNCPGEHVLTNQYILDDQVIYENGL
                     VNDLKLVSPTTFNLEDCLISKRYNQLTESHKLVKKIKFVDEVMDYTLIVDRNCQVKCV
                     PLVQTNSHKELSNSIAFDIRSQDFHYLIGVHMDDIIFGDKLYLMQGIKCKGKVIVYDK
                     NNGKMSRLKN"
     gene            10026..11027
                     /locus_tag="VBC74_000161"
     CDS             10026..11027
                     /locus_tag="VBC74_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6941145.1"
                     /translation="MSKKMTINDIAQLSKTSKTTVSFFLNQKFEKMSDETRQRIQEVI
                     DETGYRPSTIARSLNSKKTKLLGVLIGDITNTFSNQIVKGIEHITKQKGYQIIVGNSN
                     YDAKSEEDYIENMLNLGVDGFIIQPTSNFRKYSRILKEKKKPMVFFDSQLYEHKTSWV
                     KANNYDAVYDMTQECLNRGYKKFIMITADTSLLSTRIERASGFMDALKDNGFGYDTLV
                     IEDDDHSKSDIEDFLKAVVPDKEETLVFAPNCWALPMVFTAMKNLNFDMPRVGLVGFD
                     NIEWTDFSSPKVSTIVQPAYEEGEQVAQILINRIEGDDSVDNQQIVDCQMFWKESTF"
     gene            complement(11099..11713)
                     /locus_tag="VBC74_000162"
     CDS             complement(11099..11713)
                     /locus_tag="VBC74_000162"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO6941146.1"
                     /translation="MSAIDKLVKASHLIDMNDIIREGNPTLRKVAEEVTFPLSEKEEI
                     LGEKMMQFLKHSQDPIMAEKLGLRGGVGLAAPQLDISKRIIAVLVPNVEDAQGNPPKE
                     AYSLQEVMYNPKVVSHSVQDAALSDGEGCLSVDREVPGYVVRHARVTIEYFDKTGEKH
                     RLKLKGYNSIVVQHEIDHIDGIMFYDRINEKNPFAVKEGLLILE"
     gene            complement(11779..12423)
                     /locus_tag="VBC74_000163"
     CDS             complement(11779..12423)
                     /locus_tag="VBC74_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEO6941147.1"
                     /translation="MDIENYCQDYCLETIFLKHYFKDLQVITLTAGQSVCKQGEQLEY
                     LHYIVKGRFKIVRRLFNGKEHILDIKTKPTLIGDIELLTNRQIVSSVIALEDITVIQL
                     SLKGRKEKLLTDATFLLKLSQELAQAFHDQNIKASTNLGYTVKELLASHILAIEEQGC
                     FHLELSSLADSFGISYRHLLRVIHDMVKEGLIQKEKPKYFIKNRFALESLNIQP"
     gene            12535..13698
                     /locus_tag="VBC74_000164"
     CDS             12535..13698
                     /locus_tag="VBC74_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6941148.1"
                     /translation="MKIFMEKLSLLSLSLILLSTFSTSPALPQMISYYRDKGLPSPQV
                     ELLFSIPSMAIIFILLITPWLSKKLSEKHMIIFGLLLTALGGGLPVVSQNYLLVFVSR
                     LLLGSGIGFINARAISVISEYYQGKERRKLLGLRGSFEVLGNAGLTALVGLLLTFGWS
                     KSFMIYFLALPILVLYLVFAPKKVVKDTNDKIKTKGQKIPKADLTYIVALAILAGFVI
                     TINTGINLRIPLLVVEFGLGTPAQASLVLSAMMLMGIIAGMSFGQLIAMFHKQLIPIC
                     LVLFSLTLLGVGLPSNLMVLTISAMASGFLYSLMVTAVFSLVADRVEYSLVGSATTLV
                     LVFCNIGGASAAILLSCFDHLLGQINAVFYVYAILSLAVGMIYFLKRLFFKKM"
     gene            complement(13744..14727)
                     /locus_tag="VBC74_000165"
     CDS             complement(13744..14727)
                     /locus_tag="VBC74_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6941149.1"
                     /translation="MTEKYYNWATLGTGVIANELAQALEARGQKLYSVANRTYDKGLE
                     FANKYGIQKVYDHIDQVFEDPEVDIIYISTPHNTHISFLRKALANGKHVLCEKSITLN
                     STELKEAIDLAETNHVVLAEAMTIFHMPIYRQLKTLVDSGKLGPLKMIQMNFGSYKEY
                     DMTNRFFSRDLAGGALLDIGVYALSCIRWFMSEAPHNITSQVTFAPTGVDEQVGILLT
                     NPANEMATVSLSLHAKQPKRATIAYDKGYIELFEYPRGQKAVITYTEDGHQDIIEAGK
                     TENALQYEVADMEEAISGKTNHMYLNYTKDVMDIMTQLRQEWGFTYPEEEK"
     gene            14875..16770
                     /locus_tag="VBC74_000166"
     CDS             14875..16770
                     /locus_tag="VBC74_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HEO6941150.1"
                     /translation="MIKYQDDFYQAVNGEWAKTAVIPDDKPRTGGFSDLADDIEALML
                     STTDKWLADENKPSDTILNHFIAFHKMTADYQKREEVGVSPVLPLIEEYKGLQSFSEF
                     ASKVAEYELEGKPNEFPFGVAPDFMNAQLNVLWAEAPGIILPDTTYYSEDNEKGKELL
                     ALWRKSQEDLLPLFGLSEQEIKDILDKVLALDAKLAQYVLSREESSEYVKLYHPYNWE
                     DFTKLAPELPLDAIFQKILGQKPDKVIVPEERFWTEFASDYYSESNWELLKADLILSA
                     ANAYNAYLTDDIRIKSGVYSRALSGTPQAMDKKKAAYYLASGPYNQALGLWYAGEKFS
                     PEAKADVEHKIATMIDVYKSRLEKADWLAQSTREKAIVKLNVITPHIGYPEKLPETYT
                     KKIIDPKLSLVENATNLDKISIAYGWSKWNKPVDRSEWHMPAHMVNAYYDPQQNQIVF
                     PAAILQAPFYALEQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNNWWTDEDFK
                     AFKKLTDKVVEQFDGLESYGAKVNGKLTVSENVADLGGVACALEAAQRESDFSARDFF
                     INFATIWRMKARDEYMQMLASVDVHAPAQWRTNITVTNFEEFHKEFDVKDGDNMWRPV
                     EKRVIIW"
     gene            complement(16820..17773)
                     /locus_tag="VBC74_000167"
     CDS             complement(16820..17773)
                     /locus_tag="VBC74_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEO6941151.1"
                     /translation="MKKLTKGSHIRVLSPSSSIERLGGFEANLLAKEVLEKLGFQVSF
                     SKHYLENDILYSASIASRVEDLHEAFADPSVDAILATIGGFNSNELLPYLDYDLISKN
                     PKIICGYSDSTAFLNAIFAKAKIQTYMGPAYSSFKMKEGQPYQTQAWLTAMTENHYEL
                     WPSEEWSSDPWYDPSKPRQFFPTEWKIYNHGKASGTIIGGNLSTFGLLRGTPYAPKIE
                     RYVLLIEEAEESNFYEFDRNLAAILQAYPHPQAILMGRFPKECGMTPQVFEYILSKHA
                     IFKEIPVIYDMDFAHTQPLLTVTIGAEISVDTTTLSLSIKE"
     gene            17940..18176
                     /locus_tag="VBC74_000168"
     CDS             17940..18176
                     /locus_tag="VBC74_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941152.1"
                     /translation="MSQLSLEERLNRLKDKYLSSKNQEFSNVHLSKVKKKKKKLIILE
                     KERCRCRLEREDTKKIDDKIEKLKKELIDVMKEE"
     gene            18178..20247
                     /locus_tag="VBC74_000169"
     CDS             18178..20247
                     /locus_tag="VBC74_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEO6941153.1"
                     /translation="MEETILIVSFLLFLILSNVINRIFPKLPLPFIQLVFGILSGLVF
                     HKSQVHIDPELFLAFVIAPLNFREGQESDIGSFVKYRTIILYLILPTVFLTAIVVGYV
                     AGHLLPVSLPLAACFALGAALGPTDAVAFISIAKRFQFPKRAENILKLEGLLNDASGL
                     VSFQFALTALVTGYFSLAKASLKLALAIMGGFLIGLLFAFLMRLCLTVLEKFDAADVT
                     GALLLELTLPFVAYFVADLLGFSAIIAVVVAGVMQANRLKKVTLFDAQVDRVTSVIWE
                     TLNFILNGLVFLIFGRELIRIIGPLLTSNAYSNFDLISIVVLVTCTLFLVRFLAVSRF
                     YAWRSFKYHKSFKKYWREIQLLTFSGVKGSVSIATILLLPKHSVIGELGYSLILFTVG
                     AVTLMSFLTGLLVLPKLAPPLQVKDDYLIRLSILTKVLSVLEEDGKSSENQASFYAVI
                     DNYNSRIRHLILEQESSDIKKDLAELQLMMLSIESDGLEAAYRYGNISIKEYRIYQRY
                     LKYLERQVNRSFVSNITYSLTILMRVIRGIVHEFLTFGQGIRNRKKHFKNRKRLLAEV
                     NRQHLTDLYLSNNDLILETLEDMEGYYNATLIDFLKNERIRDAELIKSGLFVERVIAR
                     ITPDILNETLRGYYLERRTISEFERDGLISSKESQILRDNVNELESYSLRDSQSSIPY
                     QLLDLKK"
     gene            complement(20391..21458)
                     /locus_tag="VBC74_000170"
     CDS             complement(20391..21458)
                     /locus_tag="VBC74_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6941154.1"
                     /translation="MAYYRKRDNGWEYRISYKDESGKFRQKSKSGFKTKKLAQAAARD
                     IEKKLSQNILTDGEVTLYDFVKTWSEVYKRPYVKDKTWETYTKNFRHIKTYFKDIKVK
                     DITPLYYQKRLNEFGEKYAQETLEKFHYQIKGAMKVAVREQVIHFNFADDAKVKSQIE
                     SRAEENDFLEESEYKALLSLTRENIQYVSYFTLYLLSVTGLRFSEVMGLTWSDIDFKN
                     GILDINKSFDYSNTQDFADLKNESSKRKVPIDSNTIDILREYKKNHWQANIKNRVCFG
                     VSNSACNKLIKKIVGRKVRNHSLRHTYASFLILNGVDIVTISKLLGHESPDITLKVYT
                     HQMEALAERNFEKIKNIFLVA"
     gene            complement(21582..21986)
                     /locus_tag="VBC74_000171"
     CDS             complement(21582..21986)
                     /locus_tag="VBC74_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HEO6941155.1"
                     /translation="MALFGGKDKEIEVELFTSEPNERVFEFKKSKTIVRIDDYFIRIA
                     RKSNMSNMLLHGLDGEKSILLSEITAYQLKEPSSTVGYLQLVYPGSSDTKGGVFDAVK
                     DENTVTFSKDEKIAILELKNAIENALKNKVKK"
     gene            complement(22008..22754)
                     /locus_tag="VBC74_000172"
     CDS             complement(22008..22754)
                     /locus_tag="VBC74_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEO6941156.1"
                     /translation="MKYTPLSREQYFDYHNKAYQIISSIDKMKENVSYQDVIKHFEQN
                     YPILFNFLDYDEMKERYPELPDYHPTDKDIKYRGLVSNRTVTYTDRVLCESCAGLTVP
                     DLDLGRYIIYINQHTNTKGRVIFTILHELSHIHCHLNQTENQPIYMSLMSKNASEKYP
                     KELIPIEKEADTVASILYLTDERLKKALTTRESFEDIQRETDISKPALHNRLMNYLIY
                     NLQYAESYALKLVLNYRQGGGQIFNILRLQ"
     gene            complement(22763..23143)
                     /locus_tag="VBC74_000173"
     CDS             complement(22763..23143)
                     /locus_tag="VBC74_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6941157.1"
                     /translation="MFFTFEKIKELADKQGISLNQLEEQLDFPRNTIYNMKKSTPNVE
                     RVSKIADYFNVSTDYLLGRTENPKIADGQDNTAVDDLTQQAIVLFRKETEGLSASEKE
                     RFNSALEGLMKTARQLIQDDSNWK"
     gene            23431..23595
                     /locus_tag="VBC74_000174"
     CDS             23431..23595
                     /locus_tag="VBC74_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941158.1"
                     /translation="MGIGSMTVTVKVTNMDKFIELSKEFNKKARELEELAHELKSFDF
                     EGEVVSTDSN"
     gene            complement(23578..24048)
                     /locus_tag="VBC74_000175"
     CDS             complement(23578..24048)
                     /locus_tag="VBC74_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941159.1"
                     /translation="MNLNQIRIIEACHKFLIGITNFEEELQDDVLVYRYKGNLVSFET
                     YQEYEQRSFVDYNLKYGYLDDTRTYIDDRADLIAAFPSEEHLRALQRVNDAEQARVQI
                     FKLLSQVNLDSLSEKNSHIKKDNFGYDFFNFATKEEYPVYLFSDDESFELVAIS"
     gene            24109..24321
                     /locus_tag="VBC74_000176"
     CDS             24109..24321
                     /locus_tag="VBC74_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6941160.1"
                     /translation="MSQQQKKWIQLVKDKLNEEQMTQTHLARACGVAKATISELLKYG
                     KGSVRLKNKVSDILHIDESWTDLEED"
     gene            24321..24662
                     /locus_tag="VBC74_000177"
     CDS             24321..24662
                     /locus_tag="VBC74_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001123159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941161.1"
                     /translation="MPITREMTITEINVLNAIKNSATYDLPIQARELRQQLGLSKRSL
                     EAVIENLRVIYKQPIVAKKKQPSGYYLPRNEEERNDGLAPYKRQILTEQNNLAAIMSV
                     NLEEYWATEKA"
     gene            24709..25239
                     /locus_tag="VBC74_000178"
     CDS             24709..25239
                     /locus_tag="VBC74_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001006994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEO6941162.1"
                     /translation="MNEVWKDIPIVKGYYQVSNLGRVRSIGRTVNAKQRTRKTKGRIL
                     KQSLSSGYAIVTLSVNGLRKSIRVHRLVAEAFIPNPINKRTINHIDENKLNNRVDNLE
                     WATDKENANHGNRTTKSSLGRCKPVEQFTLEGEFINTFDSIKSASMKTGISSQRITAT
                     AMGHQKQTHGYKWRYA"
     gene            25239..25523
                     /locus_tag="VBC74_000179"
     CDS             25239..25523
                     /locus_tag="VBC74_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO6941163.1"
                     /translation="MNDLMIQMLDQFEAGLMDRTMKVMHVVTDEKYRFPLELNKSQCA
                     LMLLGTKDTGTFDERFNCHKDFPRIPNAREKYPRDEVIEWYHKNWQRTVI"
     gene            25547..25765
                     /locus_tag="VBC74_000180"
     CDS             25547..25765
                     /locus_tag="VBC74_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941164.1"
                     /translation="MNNLQIIAVGTLISVTLIESLISNIKLRKALKNKQTAGTVIAQY
                     IPQRGFIDYKTGRRVDIDPETKKEVFVD"
     gene            25774..27105
                     /gene="dnaB"
                     /locus_tag="VBC74_000181"
     CDS             25774..27105
                     /gene="dnaB"
                     /locus_tag="VBC74_000181"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEO6941165.1"
                     /translation="MDELKVLPHDIQAEQSVLGSIFIKPEKMIEVAEYLKPNDFYRPA
                     HKILFKAMVSLADRGEAIDIVTIKSTLESTDELGMVGGISYIAEIVNAVPTSSHAEHY
                     AKIVAKKAQLRSIIDNLSDSIGNAYDEDMDIDEIIAKAERSLIEVSQASNKSSFRPIH
                     DVLLENHSKIEERSNNTSQITGIETGFYDFDKLITGLHEDQLIVLAARPAMGKTALAL
                     NIAQNVATKSNKAVAVFSLEMGAESLVERMLSAEGTIINHHIRTGNLTVNEWQRLIYA
                     QGQLAEAPIFIDDTAGVKITDIRARARRLSQETDGLGLIVIDYLQLIQGSRSDNRQQE
                     VSEISRQLKIIAKELKVPVIALSQLSRGVEQRNDKRPIMSDLRESGSIEQDADIVAFL
                     YRDAYYQDKKEGQPENDITELIIRKNRHGNLGTVKLYFHKEYTKFSSVEEE"
     gene            27107..27709
                     /locus_tag="VBC74_000182"
     CDS             27107..27709
                     /locus_tag="VBC74_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941166.1"
                     /translation="MKKFDHYFSNHIAVKNESDTVTIVDYYNPEKGYEYNLRYIFDRK
                     NSSLAITGDFGELVARNFYNMGEWDTFYNHYTGHLGYFLEKLLCSSRPVHYYDTDKAK
                     REILNEFFDGADYDDLDFDDEFLLDQLFDNFDEDKGFTYILPEAFERFSEDMEEYEVY
                     EFLSRAGRYVNDVFELYLDAYKRAYEYLTKEVDDGTTQNV"
     gene            27687..28433
                     /locus_tag="VBC74_000183"
     CDS             27687..28433
                     /locus_tag="VBC74_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HEO6941167.1"
                     /translation="MAQRRMFSRKITETDRFLEMPLSSQALYFHLNMGADDEGFIDKA
                     KTIQRTIGASDDDMKLLIAKGFLIPFDSGVVVIRHWRIHNYIQSDRFQSTLYQSEKAQ
                     LEYDKSKTSSLKPIGNCIQNVSKMETQVRLSKGSLDKDSLTTYPTVSDNEEEDIPYKE
                     IISYLNEKANRNYRPNIQKNKTLIKARWSEGFRLDDFKHVIDTTVKDWSGTKYEKYLR
                     PETLFGSKFEGYLNQAPRIKTETIDGRLGF"
     gene            28434..29255
                     /locus_tag="VBC74_000184"
     CDS             28434..29255
                     /locus_tag="VBC74_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEO6941168.1"
                     /translation="MNPFKNFETRQVLEETCEVHGCQLWLTKVPIKGRLEELKQCPEC
                     TKAAINIFENKLNSQSKINSKLADTYAVFERDSLVSDKLRGKSLENYEIKDDIDQQAI
                     NYAKRMEQFYRQDRTGNAIVTGPSGVGKSHLTYGLAKFMNEQFKAYESPKSVLFISLV
                     SLFTKIRESFKADNGYRQAEMIELLTSVDYLFLDDLGKESRKGDSQNNEWTHQILYEI
                     LDNRSNTIINTNLSSKEIKALYADNYGNGALSSRILEGVTGNSFAYPKDMEDRRY"
     gene            29255..29503
                     /locus_tag="VBC74_000185"
     CDS             29255..29503
                     /locus_tag="VBC74_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941169.1"
                     /translation="MSYKATFMLYGYKAVPIGLFDTKELAAQAIVDHIIENSGIPSPR
                     VVATMKIRSKEIRIDYGSKTCYYLITLINNKTEEKQHD"
     gene            29496..29990
                     /locus_tag="VBC74_000186"
     CDS             29496..29990
                     /locus_tag="VBC74_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEO6941170.1"
                     /translation="MTKTMTLEEKVEQWFIDRNLHEANPVKQFQKMMEETGELYSGIA
                     KDKSEIIYDSLGDMQVVLIGIEQQIKNGAQIEASPQDMELLLLASSLGEMAQKLYKHI
                     FHNETKTPLIRPELSLLHSTIHAIAIHNLTTADDCLSIAYEEIKDRKGKLINGIWVKE
                     EDLK"
     gene            29987..30316
                     /locus_tag="VBC74_000187"
     CDS             29987..30316
                     /locus_tag="VBC74_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEO6941171.1"
                     /translation="MKNMNWKAFFNNLKAFASVIIITVFVLGFNYLFYEIGYQKGHQE
                     ADRVIIYVADNAGAEMFGKITDKEIIEGRHTVTAGAYGKFLVTEEQYNEITVGDDIPD
                     YLKGRGN"
     gene            30316..30735
                     /locus_tag="VBC74_000188"
     CDS             30316..30735
                     /locus_tag="VBC74_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEO6941172.1"
                     /translation="MTKCNFEAGHKPIDTIGQLAKEVLDGKYGDGEERRRNLGCLYDL
                     VQTRVNYMVHERDSKFTGIKIYDNVNKPSHYQGKNGMEAIDVVRNFIGQLTGVSAFYW
                     GNSMKYLLRFQGKNGLEDLKKARKNLDWLIEEMEKEK"
     gene            30732..30938
                     /locus_tag="VBC74_000189"
     CDS             30732..30938
                     /locus_tag="VBC74_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941173.1"
                     /translation="MKKSIGILEFKEFDAFVGGKVMPVYLHSANPNVPTTNKNEAMRV
                     TKKRYRKEFSDKYIFRKVGEVDDQ"
     gene            30928..31344
                     /gene="ssb"
                     /locus_tag="VBC74_000190"
     CDS             30928..31344
                     /gene="ssb"
                     /locus_tag="VBC74_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO6941174.1"
                     /translation="MINNVVLIGRLTRDVELRYTPSNIANATFNLAVNRNFKNAAGDR
                     EADFINCVMWRQQAENLANWTKKGMLIGITGRIQTRSYENQQGQRIYVTEVVADSFQI
                     LEKRDNSTNQASMDDQLPPSFGNSQPMDISDDDLPF"
     gene            31354..31614
                     /locus_tag="VBC74_000191"
     CDS             31354..31614
                     /locus_tag="VBC74_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941175.1"
                     /translation="MNEYKKPTYIVIQEAMAERIRFLEDELYNRAYKDIERLEVENDR
                     LKVRCLDLQLENADYVWDDMCRSAIARAKSENRRPHRRWRGR"
     gene            31611..31958
                     /locus_tag="VBC74_000192"
     CDS             31611..31958
                     /locus_tag="VBC74_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6941176.1"
                     /translation="MKLRLEELRNKLGLSRRDVHEATGISMNTLLHYEKGGTPSIGQV
                     ENIAKAYNINPAWLLGWIDEGQQPQPLETVVEKIIYVEKEGCRLPPYWNNDNNGRIIK
                     WKESRRAFQRRTI"
     gene            31955..32419
                     /locus_tag="VBC74_000193"
     CDS             31955..32419
                     /locus_tag="VBC74_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941177.1"
                     /translation="MTKKTASKTRRDFLEFELEAKYLKIDKLIGQRRHELERIYAVKN
                     LTMPGIDDSGASRSGTSTNTSENLAVAYASDPMILKLEEFQNAISELLEVLEPDDKKI
                     FHLRWGEHTGYDWIQVWHIMENGETGYLYRHSKQIYRRREVILDTLAKLLFM"
     gene            32529..33071
                     /locus_tag="VBC74_000194"
     CDS             32529..33071
                     /locus_tag="VBC74_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6941178.1"
                     /translation="MNIVEPLRDKDDIQAMKDYLSSWNEKYYMLFLLGINTGFRVGDI
                     LKLKVKDVQGWHIKVREQKTGKYKSIKMTRPLKNELREFVKDKELHEYLFQSRVGKNK
                     ALSYKTVYWFLKRAAEDLGIDNVGTHTMRKTFGYHYYKKYKNVADLMSLFNHSSPAVT
                     LIYICVRQDELDTKMSNFSL"
     gene            33361..33648
                     /locus_tag="VBC74_000195"
     CDS             33361..33648
                     /locus_tag="VBC74_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941179.1"
                     /translation="MKDENAFDGIRLEAGSTLEIYLTKNDLEHIANGYEVTLDIKPNE
                     TVNKIVIKPSFVNNILNPLINYDKRIVSEADLKFRDISREVARDSFALGSM"
     gene            33648..34007
                     /locus_tag="VBC74_000196"
     CDS             33648..34007
                     /locus_tag="VBC74_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEO6941180.1"
                     /translation="MGYRNPKHSDWFRAWQIKFYNSKPWRTLRNKIRKAKRMRCDMCG
                     RLIHGKSIVDHIIEIDETNYQDESITLNEENCQLLCLECHNTKTFQSKINLNLDNRNI
                     NLFLIFLFLNFCGLPPF"
     gene            34095..34580
                     /locus_tag="VBC74_000197"
     CDS             34095..34580
                     /locus_tag="VBC74_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941181.1"
                     /translation="MILKINEVLEKLGISRATLTRYRKKLGIFEETRSNITKSQFKEL
                     EKLANQRQKYTRQERVELSRKTFKLIPKEKMLEISDNDSVVLKNLKTQYNHNQKVIEN
                     FQLEINKVINDGELPDKYLLDGMEKYQKLNMQIMSTIEKQSPQGDSLKEMIQEKLARY
                     G"
     gene            34573..36285
                     /locus_tag="VBC74_000198"
     CDS             34573..36285
                     /locus_tag="VBC74_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEO6941182.1"
                     /translation="MVEMRYFDKYAQLIYTGKIRICKLTMKSIRRVERYKEQYLFKQE
                     EADKRIEFIEEECSNTKGLAGKLRLALPQKVWLETTWGFYHTVEVTKTNPDTLEEYTD
                     YEERRLIHEVPIIVPRGTGKTTLGSAIAEVGQIIDGEWGADIQLLAYSREQAGYLFNA
                     SRAMLSNEESLLHYMREADILRSTKQGILYETTNSLMSIKTSDYESLDGTNAHYNIFD
                     EVHTYDDDFIKVVNDGSSRKRKNWITWYISTNGTKRDKLFDKYYNIWVDILDDKIIND
                     SVMPWIYQLDDVSEIHDPDMWQKAMPLLGITTEKETIARDIEMSKNDPAQQAELMAKT
                     FNLPVNNYLAYFSNEECKGWSDKFDESLFVGDDERNARCVIGIDLSDVNDICSISFMV
                     VRGEERHYLNKKFMPRHTIETLPKELRDKYTEWELSGMLHVHELDYNDQAYIFEELRQ
                     FMSDNRILPVAVGYDRYNARELIRLFNDYYGDICHDIPQTVKSLSNPLKVYKEKAKMG
                     KIIFDDPVATWNHANVRVKIDANNNIFPNKEKAKEKIDVFASQLDAFICYENFKEDLS
                     YYFD"
     gene            36294..37436
                     /locus_tag="VBC74_000199"
     CDS             36294..37436
                     /locus_tag="VBC74_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO6941183.1"
                     /translation="MNNYLKNLKEVFARIFRPNNRKSTRTYLQRSISYWRRNSIYLDN
                     IYNKISTDTAQLRFKHVKITRNPGGVDSMVWYEHSDLAEVLTVSPNPLEVPVVFWSNV
                     TRAMLRDGVAVVVPRWKNGRLVEIWLAKKTVTWTAESVELMLDDVAVELPLTDVWVFE
                     NPKLNVTAQLNQITELIDINLNALTEKLSDGNSSLRGFLKLPTKAADEHLKQQARDRV
                     DSMLDLAKNGGIAYLEQGEEFQELSKDYSTASKEELEFLKSQLYNAHGINEKLFTCDY
                     TEEQYRAYYSSVMKLYQRVYSEEINRKYFTKTARTQGNKLLVFFDMADMISFKDLVEG
                     GFKSKYAGLMNSNEFRETYLGLPGYEGGEVFETNLNAVRIGAEESE"
     gene            37486..38028
                     /locus_tag="VBC74_000200"
     CDS             37486..38028
                     /locus_tag="VBC74_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEO6941184.1"
                     /translation="MEKLKTFVVKSVEDESADFHFEAYASTYDNTDREGDVMAKGCFD
                     NTLKSKAVVPMCLNHDRNCVIGKHELSVDEKGLRTRSTFNLSDPEAKKTYDLMKMGAL
                     DSLSIGFFIKDYEPIDAKQPYGGWIFKEVEIFEISVVTVPANPQATVDNIKEFDMSAV
                     DKRIAQVNMRQEIMSKLAKI"
     gene            38039..39301
                     /locus_tag="VBC74_000201"
     CDS             38039..39301
                     /locus_tag="VBC74_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEO6941185.1"
                     /translation="MKKSLIELLEARQKATDELAEVKLKKATIEAKMKSSTIEDDDLE
                     QLKTDAESLVSQATAIKETIAGLDSDIEETEEELSKAAKIIKEKQKGNTPMDYLKTKA
                     AALDFVRILMDNEGSANSARKAWEANLVEKGVTNLTKILPEPVLIAIQDAFTNYNGIL
                     NHVSKDPRYAVRVALQTQVSQAKGHKAGKTKKDEDFTFLDFTINSATVYIKYAFEYSD
                     LKKDTTGAYFNYVMKELAQGFIRTIERAVVIGDGKSNSAEDKITEIKSIAEETEANLF
                     ETQEINVAGVFDNAVLETLVAGIDKMVPNTTPILVTSKAIARKLKLVKDAEGRYIDPQ
                     PFAPIATNGNVIAGFQVYIYDWMDGATNPIIAFADQAYKMIGDEAAADRFDDYDVTVN
                     RRHIELASVMGGRLAQYKSAVKFTNQAG"
     gene            39322..39657
                     /locus_tag="VBC74_000202"
     CDS             39322..39657
                     /locus_tag="VBC74_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941186.1"
                     /translation="MTILKTIKEMVEVDVEEDIFDVQLLRYINSGISYLQRNAIPVIR
                     IDKETELEGWTDIKEEDRETVLDWLHLRCVQRFDKSLMTGSAATMEWIDGEMTNLLYQ
                     LKAIYEVKT"
     gene            39654..39959
                     /locus_tag="VBC74_000203"
     CDS             39654..39959
                     /locus_tag="VBC74_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HEO6941187.1"
                     /translation="MKSSRVAIILCYDERVEVEKGVWEKKTVEKKVKAKKEKIYQRRL
                     DKAMADGQVLTARFSVRSNYVTDTLDYVKYNGKEYKVNIGTESDDGHYTIIELGELK"
     gene            39959..40306
                     /locus_tag="VBC74_000204"
     CDS             39959..40306
                     /locus_tag="VBC74_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941188.1"
                     /translation="MAKKFFTRQDIQSILETNPLKAKVFYMEREEKSSPDNVILYYRL
                     TPGSSITADDRVHMRKVTLQVSHYHKKKLDSIENLMLSNFMCEPNQLNLKQPDTDYLL
                     TTYRLEVFTNGRW"
     gene            40293..40637
                     /locus_tag="VBC74_000205"
     CDS             40293..40637
                     /locus_tag="VBC74_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEO6941189.1"
                     /translation="MGGGSVKMTPLKVDIKNQVLENIKKAAQSTENDIRTKSPRRNGV
                     YEKGWTHDIIDDAAVVHNNGKEKSLSHLLENGHATKNGGFVAPREHIRPAYIKNKEKF
                     LNDMKSIKITPK"
     gene            40652..41335
                     /locus_tag="VBC74_000206"
     CDS             40652..41335
                     /locus_tag="VBC74_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6941190.1"
                     /translation="MTYQYDTREVTHGNANGFYAKIAKTDAGALDLQKPYPFTGLRST
                     SFETSQESNAYYADNVEHVRLQGKKSTEGSITTYQIPKQFMIDHLGKKLTNSTPPALI
                     DTGVNTNFIWGYAETVTDEFGAEIEEFHIWTNVKASAPKGSTSTDETSATPKEIEIPC
                     TASPNNFIVDSEKKPVSEIVWRDDSKGTVRGKFDKLFADKSPGKLIDFINEALGTTTT
                     PTTTVSGGS"
     gene            41335..41811
                     /locus_tag="VBC74_000207"
     CDS             41335..41811
                     /locus_tag="VBC74_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941191.1"
                     /translation="MIKKELSFTAFDSYGEEREHTETVRFLYSLPAIKMYEQRTGRNF
                     FDDNQKALTAYTQLALATGVNGNLSDLTDEEKIKMMPLLMEPDFMNFLTEVIPCLYGE
                     VENGRLVQNELTAETASLAPWFGDLIDIGFFSDLFYEFNRSRAKVPQDKKKPFQKL"
     gene            41997..44732
                     /locus_tag="VBC74_000208"
     CDS             41997..44732
                     /locus_tag="VBC74_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEO6941192.1"
                     /translation="MTETFEGLYVKFGANTVEFDRSVKGINTALSSLKKDFNNINRQL
                     KMDPDNVDLLNRKLVNLQEQARVGAMKIAELKKQQKALGESEVGSAQWNKLQLEIAKV
                     ESQMKIVDKAMESTKKHIEDVGDPKSILNLNKELDNVAKELDIVNQKLELDTDNVELA
                     EQKMKLLGKQSELVGDKVQELKKKQAALGDEKIGTEEWRQLQNEIGQAEVEVLKIDRA
                     MDILGESSRSATGDIKEATSYLRADVMMDVADKAGQIGQKMVDAGKMTVDAWSEIDEA
                     LDTVTTKTGLTGDALAELQEIAKDIATGMPTSFQNAGDAVGELNTQFGLTGEKLKSAS
                     ELLIKYAEINETDISSSAISAKQAIEAYGLTAEDLGMVLDNVTKAAQDTGQSVDTIVQ
                     KAIDGAPQIKGLGLSFEEGAALIGKFEKSGVDSSAALSSLSKAAVIYAKDGKTLTDGL
                     NETVSAIQNSTSETEALSIASEIFGSKAAPRMVDAIQRGAFSFDDLAEAAKSSSGTVS
                     TTFDETLDPIDKLTQYSNQAKEGMAELGGKLLETVIPALEPLMGMLESSVNWFTSLNE
                     TDQQTIVILGLVTTAVMMLLGAIAPLVIAIGAIGAPVGIVVAAIVGAIAVITLIIQAI
                     MNWGAITEWLQSTWDSCAAWLSELWTNIVTTATTAWSNFTAWLSGLWSSVVSTGQSLW
                     SSFTSSLSNIFSSLITGAQSLWSSFTSTLSNLWSGLVSTGSNLFNNLSSTISGIFNGI
                     LSTASNIWNSIKSTISNAIDGAKNAVSNGVNAIKNLFNFQIKWPHIPLPHFRVSGSAN
                     PLDWLKGGLPSIGIDWYAKGGIMTKPTLFGMNGNRAMVGGEAGAEAILPLNKSTLGAI
                     GQSIANTMNTSNNINVNFSGVTIREEADLNRLANVVGNRIAEELQRKTNLRGGMA"
     gene            44729..45451
                     /locus_tag="VBC74_000209"
     CDS             44729..45451
                     /locus_tag="VBC74_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6941193.1"
                     /translation="MTKINELTIDGVKTSSFKCDVLVETRPNVIVSSSKTALLEHDGI
                     SGAVVQSNRHRGLIEKPYHITLIEPSDEEIYRFSALLNREKFWLENEQEPTIRLWCYK
                     VDSFEIGKDEFGAWVVDVTFICHPTKFFKTTDTQTLTGNGVLRVQGSALAFPKITVVG
                     QSASETSFTVGNQVIKLEKLSESLVMTNDPDNPSFKTASGKLIKWAGDFITVDTAKGQ
                     NVGVVLGAGITSLKFETVWGWA"
     gene            45452..49126
                     /locus_tag="VBC74_000210"
     CDS             45452..49126
                     /locus_tag="VBC74_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001918212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6941194.1"
                     /translation="MLFLLDKDVRTVKWNGIPLHEASSAIVKEETNSDFYLTVRYPIT
                     DSGIYQLIKEDMLIKAPTPVLGAQLFRIKKPIENDDSLDITAYHVSDDIMKRSITPVS
                     VVGQGCAMALSQMVQNAKTGLGDFSFTSDIMDSRTFNTTETKTLYSVLMDGKHSIVGT
                     WEGELVRDNFALSIKRSRGADRGVVITTHKNLKSYQRTKNSQGVVTRIHARSTFKPDG
                     AEDEVTLRVSVDSPLINSYPYINEKEYENNNAETVEDLRKWAEAKFTNEGIDKVSDAI
                     EIEAYELDGQVVNLGDTVNLKSRKHSADLYKKAIAYEFNALTEEYISITFDDKPGVGG
                     SGVSSGLSNVADAILVASATAQDVAVQRAVKNANAAFDAEFGKTKTKINDDIEIAKAK
                     VESFKSELSNRMDNQLLPLATEAKNLASQAQADLTRKEIELRAELNRQVTSTETVKIS
                     LTNLSHNMDIIKQKALNDLRDAETRLKEADSVQQLATKRVEDKLTGLSTKLESFSVGG
                     YNYVIDGGEPKELMANFYGKTYDINPQLLERTSQATLSFSYEAESTSRLEVRLYKKMH
                     TGDTSKITIIVMPNFDLSPGKGFISQSFDLGGVMPDPRNQAWLVMRGTNANPLTLSKV
                     KLERGTVATDWNNRDETLKASFAEYKQTVDENLANLRTSTETLAGQLTSAESSIRQTS
                     ESFSNRLVSLETYKDSESNRASRYFEASKSETAKQLSALRTEVNGSYVDKSTYEENAR
                     GLTRRFESLSSGTQNYALGTERDVYLNVSGTTVYDLYSFGTTLAIKGIEMGDTVTIAF
                     NYFADSRTAFGSYELELYGHAGKIERVGEVQRTISNRGTYTASFVANNANFRANSLKI
                     RFTDSRLKFRVTTLRVTKGTIPADWSPSPDDLKAYSDTKLEQTANEIKASVTSLDHKT
                     LKQTDITMTSEGIVLRAGKTSNDVARAIGSYFKVTPDAIALFSSLIKVSGNMLVDGSV
                     TSRNLVTGAVETGHVKAGAITGVLLAAEAVTAEKLKVDQAFFNKLMANDAYLKQLFAK
                     SAFITQVQSVTISASQISGGVIKALNNAMEIQMNSGQILYYTDQAALKRVLSGYPTQF
                     VKFATGTVSGKGNAGVTVIGSNRYGTESTNDGGFVGVRAWNGSNIDSLDLVGDKIRLA
                     SSAFDNSDGWDVRTLDSGLKITPHNRAAERNSRIEVGDVWILKGNGSYSSLRDILNSF
                     NENFSKGPNADSYTYYHSGF"
     gene            49143..49373
                     /locus_tag="VBC74_000211"
     CDS             49143..49373
                     /locus_tag="VBC74_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941195.1"
                     /translation="MNQEQQLNQALRLTVNELTAQLANESTTKNLLAIQLTEVDQEKQ
                     QLTQQNAELQARVSELEGLLDEQTQPEIIEGE"
     gene            49376..49714
                     /locus_tag="VBC74_000212"
     CDS             49376..49714
                     /locus_tag="VBC74_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941196.1"
                     /translation="MTTTENNLLDLETITEPFDLATALKYMKENGELIRCKNAVNDFY
                     MYRDMQKRPVIVNGRRQFKDVETVWAFNQWGGTTPTINMADLFNLEYYIMTFDENGNP
                     DWTEPHLEDK"
     gene            49749..50159
                     /locus_tag="VBC74_000213"
     CDS             49749..50159
                     /locus_tag="VBC74_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941197.1"
                     /translation="MPPWLIDSTVVVAMVTVLGGLFSTIITTSANRKDQLIKHQYEDI
                     KEDLSGLIDKVKTIDHTTTETKKISEITKDGTLKIQRYRLFHDLTKEISQGYTTIEHF
                     RELSILFESYQLLGGNGEIEALFEKFKQLPIEED"
     gene            50162..50491
                     /locus_tag="VBC74_000214"
     CDS             50162..50491
                     /locus_tag="VBC74_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO6941198.1"
                     /translation="MTQITDIIISSAMGILTILAGIAVQAVKVYLIKKGGEKAVLITE
                     ILAKNAVNAVEQVATETGFKGADKLTSAKAQILAELQKYNIHMSDDDLTLFVESSVKQ
                     MHDAWKE"
     gene            50495..51901
                     /locus_tag="VBC74_000215"
     CDS             50495..51901
                     /locus_tag="VBC74_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEO6941199.1"
                     /translation="MEINTETAIAWMSARQGKVSYSMDYRDGPNSYDCSSSVYYALRS
                     AGASSAGWAVNTEYMHDWLIKNGYELIAENVDWNAVRGDIAIWGMRGHSSGAGGHVVM
                     FIDPENIIHCNWANNGITVNNYNQTAAASGWMYCYVYRLKSGASTQGKSLDTLVKETL
                     AGNYGNGEARKAVLGNQYEAVMAVINGKTTTNQKTVDQLAQEVIAGKHGNGEARKKSL
                     GSQYDAVQKRVTELLKKQPSEPSKAQEVNKPTETKTSQTELTGQATATKEEGDLSFNG
                     TILKKAVLDKILGNCKKHDILPSYALTILHYEGLWGTSAVGKADNNWGGMTWTGQGNR
                     PSGVTVTQGSARPSNEGGHYMHYASVDDFLTDWFYLLRAGGSYKVSGAKTFSEAIKGM
                     FKVGGAVYDYAASGFDSYIVGASSRLKAIEAENGSLDKFDKTTDIGDGSKDKIDITIE
                     GIEVTINGITYELTKKPV"
     gene            52108..52293
                     /locus_tag="VBC74_000216"
     CDS             52108..52293
                     /locus_tag="VBC74_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEO6941200.1"
                     /translation="MTERELKKIAKKQGFSKTNFGKGSHEVWKHPDGRTVTIPKPKEK
                     DYRPGTLNNILKVLYGE"
     gene            52354..52758
                     /locus_tag="VBC74_000217"
     CDS             52354..52758
                     /locus_tag="VBC74_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEO6941201.1"
                     /translation="MKYTYLALFEVDKENGGYNISFPDFPGAFSEADSLNEAIFNARE
                     VLEIYTIMFEDEGKEFPKASSFKALASNLASDEDVIQAISVDTELVRERERSKIVNKT
                     VTLPSWLVEVGKENKVNFSQLLQKAIREELQV"
     gene            complement(52972..53042)
                     /locus_tag="VBC74_000218"
     tRNA            complement(52972..53042)
                     /locus_tag="VBC74_000218"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53008..53010),aa:Cys,seq:gca)
     gene            complement(53176..54708)
                     /gene="ahpF"
                     /locus_tag="VBC74_000219"
     CDS             complement(53176..54708)
                     /gene="ahpF"
                     /locus_tag="VBC74_000219"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEO6941202.1"
                     /translation="MVLDKEIKAQLAQYLDLLESDIVLQTDLGDNDNSQKVKDFLDEI
                     VAMSDRISLESTHLKRQPSFGIAKKGQESRVIFSGLPMGHEFTSFILALLQVSGRAPK
                     VDEDIIKRIKGIEKTINLETYVSLTCHNCPDVVQAFNIMAVLNPNITHTMIEGGMYQD
                     EVKSKGIMSVPTVYKDQEEFTSGRATIEQLLEQLDGPLDAEAFADKGVYDVLVIGGGP
                     AGNSAAIYAARKGLKTGILAETFGGQVIETVGIENMIGTLYTEGPKLMAQIEEHTKSY
                     DIDIIKSQLATGIEKKELVEVTLANGAILQAKTAILALGAKWRNINVPGEEEFRNKGV
                     TYCPHCDGPLFEGKNVAVIGGGNSGMEAALDLAGVTKHVTVLEFLPELKADQVLQERA
                     AKTDNLTILKNVATKDIVGEDHVTGLNYTDRDTNEEKHIDLEGVFVQIGLVPSTSWLK
                     DSGIELNERQEIVVDKFGSTNIPGIFAAGDCTDAAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(54726..55286)
                     /gene="ahpC"
                     /locus_tag="VBC74_000220"
     CDS             complement(54726..55286)
                     /gene="ahpC"
                     /locus_tag="VBC74_000220"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEO6941203.1"
                     /translation="MSLVGKEIIEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLDVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQGFDVLGQDGLAQRGTFIIDPDGVIQMMEINADGIGRDASTLIDKVRAAQYIRQH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            55563..56333
                     /gene="rpsB"
                     /locus_tag="VBC74_000221"
     CDS             55563..56333
                     /gene="rpsB"
                     /locus_tag="VBC74_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEO6941204.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVAEEAKRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFELLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDVIIPANDDAIRAVKLITSKLA
                     DAVIEGRQGEDADVDFAQEAQADSIEEIVEVVEGSNND"
     gene            56427..57467
                     /locus_tag="VBC74_000222"
     CDS             56427..57467
                     /locus_tag="VBC74_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEO6941205.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDLDKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVDGNVAAVIEVNAETDFVAKNDQFVTLVNETAKVIAE
                     GRPSNNEEALALTMPSGETLEQAFVTATATIGEKISFRRFALVEKTDEQHFGAYQHNG
                     GRIGVITVVEGGDDALAKQVSMHVAAMKPTVLSYTELDAQFVHDELAQLNHKIEQDNE
                     SRAMVNKPALPFLKYGSKAQLTDEVIAQAEEDIKAELAAEGKPEKIWDKIVPGKMDRF
                     MLDNTKVDQEYTLLAQVYIMDDSKTVEAYLESVNAKAVAFVRFEVGEGIEKASNDFEA
                     EVAATMAAALEK"
     gene            complement(57626..58087)
                     /locus_tag="VBC74_000223"
     CDS             complement(57626..58087)
                     /locus_tag="VBC74_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HEO6941206.1"
                     /translation="MNYIITFLISMIPLVELRGAVPFAIANGIPLWEALAIGVVGNML
                     PVPIIFFFARKVLEWGADKPYTGKFFTWCLKKGHSGGQKLEKVAGEKGLFIALLLFVG
                     IPLPGTGAWTGTLAASLLDWEFKHSVIAVMLGVILAGCIMGTLSIIGFNLF"
BASE COUNT        18610 a        10244 c        12043 g        17281 t
ORIGIN      
        1 aataaaaatc ttggattaat tttgatttta aatgcgtcta tttaaatagg tgattataaa
       61 gagatataac agtaaaacga gtcgttataa aaaataaaat cggtcaaatt agaaaggagg
      121 tttagaatgt ctaaaaaatg actattaatg atatcactca actatcaaaa cttcaaaata
      181 acggtacacc caaaagctga gacgcaggaa aaatacttct gttgaaatct aataaactat
      241 aatcagaaat gtttttaatt atgtttaaaa aatgtatttg ataggagcta agttagcctg
      301 tgttgagata ggaaaagcgg gtaagcctga taggtcattg ctaagttttt ggatataaaa
      361 atgatatcaa atctattatt tgctgaaatg atatgagaat gatgagcttc actacttata
      421 tcaatctgcg ggcaagtagt atatctttgg tgaagacatg gatcagttgt cggaagtaga
      481 acggttatgt ctacaggaag attagctaaa aattatctcg gtctgataag aaaatcgaga
      541 taatgagtct gattaagata aacggtacta cagaaaaatg gctttataaa agttcttttc
      601 ttctgtctca tgattgaggt gtgggggtgc cttgtctttt tattgtgtta attcagattt
      661 agctgtattt tgtattgttt agccatttct gtgattttat caaattcccc caaagctgct
      721 agtttattga actctccacc aataccaatg gctgttactc cagatagaaa ccagtctttg
      781 gcatttgtga gatttacccc accagtcacc ataatttgta cttgaggtaa aggtgctttt
      841 agtgatgaga tgaaagatgt tcctagagta cctcctggga atattttaat tatttcacaa
      901 ccagcttcaa gtgctgttgt tatctctgat aaggtcatgc aacctggtaa ataaggaata
      961 gcataacgat tacataattt agcagtctgt agattaaatg cgggagatac aataaacttt
     1021 gctcctgcta aaatagccat tcgggcagtt tctgcgtcta gaacagttcc tgccccaacg
     1081 acaacttgtt cttggttagt atattgtgtg actagttgtt caatgacttg gctagctttg
     1141 ctattagtat aggcgatttc gatagcagaa ataccacctt ttatgcatgc ttgagcagct
     1201 tgaaaagctt cttcttggct atttccacga atgacagcaa ctatttttcc ttgcattaat
     1261 tgcaacaaaa tctttgattt tatcataatt ctcccttcta acgaataatg tctttcgatt
     1321 gatttagtac tttgttaact gcggtaacgg tctcaaacat attgtcccct tccaaagcac
     1381 attttaaaga tgcactagcg acagcgaaat caatgactgt cttgctgtct gagtgctgaa
     1441 gtagttgata tagcgctccc gccacaaaag catctccgct tccaattctc tggtttacag
     1501 ctgtggtgat ttcttttgaa gtgacaaact cttgtttacg atttgctatg taagcttggt
     1561 agtggtttcg tccccattca tcctgtagac gtttagtatg gaaaatgact tgaaagtcaa
     1621 agtgatttat aagagaaatc attcgttctt tgatgtcctc tattgtagct tcatcgcgat
     1681 tgaagaaggt agtgtcctgt gagtcaacca taagaggttc aataccaaaa caaatatccg
     1741 caaaggtagc gaactgagaa aaaaggattt tggcattttt aggagaaatt agtttactgc
     1801 gaaaattgag atctaaggag attgttatct ctcttttttt agcttctttt aaaagaagca
     1861 gtgttatctc ttggatagac tcatccaatg atagtgtaat accactaaaa tgaaagagag
     1921 aaactccttc aaataattga tcgaaatcaa tttggtttat cctaaagtca tgtaaactac
     1981 tataatcgcg gtcgtagacc acctcacctt ttctgcaagc aaaggaattt tctaagaaat
     2041 ataacccaac acgttctcca acccactgaa tagaatgagt atcaataccc tgtgctttta
     2101 aaaattgaag aaaactatta cctatgggat tattgggtaa ggctgagagg agtttagtat
     2161 cttgcccaaa gccttgtaga gctcttgcga tattaacttc agagccacca tagaatagtt
     2221 tagttgagat actatctgca aaatagtcat tttctttggg agtgatgcga atgagaggtt
     2281 caccgaaaaa aagaatctta gacataaaag tcctttctaa aacagacgct ttagtctaag
     2341 acagatttca agtagtctgc tatattttga ttttggcaat tagcgaagaa atattcttgg
     2401 aaatgctccc cagaaatagt ttcctttaag aaatcttgat caatttcttt taaaacagag
     2461 agtaaatctc tgtaagtaat ctgttttact tcgctaagga tacgtgcatt gcgttgctca
     2521 ggtacagccc tctctttagg gtatccccct cctttttcat cttcgaaaag acgttcaaat
     2581 aaatacctca gattcaattc agctccccaa ccaaatcctt tagcaaaagg aagggaaaga
     2641 gcatttcctc cgtttacttg agagaataag taagcgtcga cagaatcagc agcgaatcca
     2701 cagactacgc caggaaagct attacaagca agcatcgctc caattcctgt cccacagcct
     2761 gtaataacaa aatctgcagc accgctatta agtaaaatag cagtcagtaa accattttgt
     2821 acataagtta gctggctttc ttcttctttg ccatacatcc cgtagttgaa aacatcaaat
     2881 cctttttttt tggaaaccgc tttcaattct ttgtagataa gagtattttt actcgcttgg
     2941 ctattttcgt tgattaatgc aattttcatt tatattactc ctttattatt gaggttgttt
     3001 accgatgtaa gctaaaatac cgccatctac atagagtatg tgaccattga taaagtttga
     3061 tgcatcgcta gcaaggaaga tagctggtgc acctaaatct tctgcctcac cccagcgagc
     3121 ggcaggtgtt ttagcgatga taaattggtc gaatggatga cgtgaaccat catcttgacg
     3181 ttcacgaagg ggagctgttt gtggtgtggc aatatagccg ggcccgatac cgttacattg
     3241 gatgttagca gaaccgtatt cagaagcaat gttcttggtt aacattttaa gtcccccttt
     3301 ggcagcagca taggcagcaa ctgtttcgcg tccaagctca ctcatcatgg agcaaatatt
     3361 gatgatttta ccatgtcctt tttggatcat tccaggtaat accgcttttg atacgataaa
     3421 gggagcattc aaatcaatat caataacttg tcgaaaatct gcagcgctca tctctagcat
     3481 aggggtgcgt tttataatac ctgcgttgtt aactagaata tcaattaccc caacatcttg
     3541 agaaattttg ttcaccattt cgttgattcc atcttcatct gtaacatcgc aaacatagcc
     3601 atgggcctta atgccaagtt ttttataggc ggagatgcct ttgtcaacga gttcctgctt
     3661 aatatcattg aagacaatag ttgctccagc tctagcaaaa gcagttgcta ttgaaaaacc
     3721 aatgccgtaa gaagcgcctg ttattaaggc gactttacct tctagtgaaa agttgtcttt
     3781 taagaattgt tcagtcataa tatgacctcc gtaaaaaaat tcaggaagta aacctgttta
     3841 catgaatagt aacaaagtaa accgattttg tcaactatag agttcttctc ctataaaact
     3901 tggcaacata tataacaaag ctttatctaa gttatgattc ttaaagataa aaaagacatc
     3961 attataaaaa atcaagtaaa ctgctcaaaa aatataaaaa agatgttgac acctaaaaat
     4021 gaaagcgcta taatatgatt ataaaagtaa accggtaaac tcaagaggag aaaatatgat
     4081 aaaaattatt attgtagcac acggcaactt tccagatggt attttaagct ctttagaact
     4141 tattgctggg catcaggaat atgttgttgg tattaacttt attgcaggca tgtcttctaa
     4201 tgatgttaga gttgctttgc aaagagaggt tatcgatttc aaagaaattt tagtattgac
     4261 tgatttgcta ggtgggactc ctttcaatgt ttcttccgcc ttatcagtag aatatacaga
     4321 taaaaagata aaagttttat cgggcttgaa cctttcaatg ttaatggaag ctgttttatc
     4381 tagaacgatg tttgaacatg tagatgacct tgttgataaa gttataacat catctcatga
     4441 aggaattgtt gatttttcta cttgccttgc tacacaaact gcagaagcaa cttttgaggg
     4501 aggcatttaa taatgatgaa aataaaaccg gtcaaggttg agtcaattga aaatccaaag
     4561 cgttttttag acagtagatt attaactaag attgaagttg aggaagccat tgaaaaagcc
     4621 ttgaagcaac tttatattaa tattgattac tttggtgaag agtatccaac gcctgcaaca
     4681 ttcaataata cttataaagt tatggataac acagaatgga caaatggttt ttggacaggg
     4741 tgcttgtggt tagcttatga gtataatcag gataaaaagt taaaaaacat agcccacaaa
     4801 aatgtattgt catttctaaa tcgtattaat aatcgtatag cattagatca ccacgactta
     4861 ggatttcttt acacaccatc ttgtacagca gaatatcgta tcaatggtga tgctaaagct
     4921 ttagaagcca ctataaaagc tgcagataaa ttgatggagc gctatcaaga aaaaggtgga
     4981 tttattcagg cttggggaga actcgggtat aaggaacact atcgcttaat tatcgattgc
     5041 ttacttaata tccaactctt attttttgct tatgaacaga caggtgatga aaagtataga
     5101 caagttgcgg tgaatcactt ctacgcttca gctaacaatg tggtgcgtga tgattcttct
     5161 gcttttcata ctttttattt cgacccagaa actggagaac cgttaaaagg tgtcacacga
     5221 cagggttata gtgatgagtc atcttgggca agagggcaag catggggcat ctacggtatt
     5281 ccgcttagtt accggaaaat gaaagattat cagcagatta tcctttttaa aggtatgaca
     5341 aactattttc taaatcgttt acctgaagac aaggtatcct attgggacct tatttttacg
     5401 gatggctcgg gtcagcctag agatacatcc gcaacagcaa cagctgtgtg tgggattcat
     5461 gagatgctta aacatttacc agaagtagat cctgataaag agacatacaa atatgctatg
     5521 catacaatgc ttcgtagtct gattgaacag tatagtaata gtgaatttat agcaggacgt
     5581 cctcttctat tgcacggtgt gtattcgtgg cattcaggta aaggagtaga tgaaggtaat
     5641 atttggggag attattatta cttagaagcc ttaataagat tctataaaga ctgggaactt
     5701 tattggtaag agtattttac taacaaaata aacaataaat aatagaaaac aaggaggtca
     5761 gaaatggcag caggaccaaa tattgtaatg acacgtgttg atgagcgtct tattcatgga
     5821 caaggacaac tttgggtgaa atttttaagt tgtaatactg ttattgtggc aaatgatgat
     5881 gtctccaaag atcatcttca acaaacgtta atgaagacgg tggtgccaga atcaattgca
     5941 ttgcgatttt ttgatattca aaaagttatt gatattattc acaaggccaa cccagcacag
     6001 acgattttta taattgttaa agatttgaaa gatgtctata gacttgtagc aggaggcgtc
     6061 ccaattaaag agattaatat tggaaatatt cataatggag aaggtaaaga gcaggtaagt
     6121 cgctctatct tcttagggat gaaggataaa gagataatta gaaaattaaa ccaagaatat
     6181 cacattgcat ttaatactaa aacaactcca acaggtaacg atggtgctgt ggaagttaat
     6241 attttagatt atatttagtt ggacaaactc actatcatat aaaggagaaa aatatggata
     6301 ttagtattct tcaggctgtc ttaattggac tgtggacggc tttctgtttt agtggcatgt
     6361 tgcttggcct ctatacaaat cgttgtatcg ttctctctct aggcgttggt gttattttag
     6421 gagatattca aacagctttg gcagttggag caatatctga actggcttac atgggatttg
     6481 gtgtgggagc gggagggaca gttcctccaa acccaatcgg tcctgggatt tttggaactt
     6541 tgatggctat tacaacagct ggaactaaag gaaaaatcac accggaagct gctcttgcat
     6601 tatcaacccc aattgcagtt ggtattcaat ttcttcaaac agcaacttat actgcttttg
     6661 caggtgcgcc tgaaacagct aaaaaagctt tgcaagcagg taatttccga ggatttaaaa
     6721 ttgcagcaaa tggtacaatt tgggcattcg ctggattggg atttggttta ggagtactgg
     6781 gtgctctttc aacgcaaact ttgacagatt tatttgctct cattccccct gttttgctta
     6841 atggtctaac cttagctggg aaaatgcttc ctgcaattgg ttttgcgatg attctctctg
     6901 taatggctaa aaaagaactc attccttata tcctacttgg atatgttcta gctgtttatt
     6961 ttggtttgcc agttctaact ccaacagcaa atggagacgg agttttaaca tcagtggcta
     7021 ctaacagtgt gcttggggtt ccaactatcg gggtagctat aattgcaaca atatttgctt
     7081 tgctcgatat tttcagaaaa ccagctgcac ctactaaaga aactaagaca gaaggagaca
     7141 accaagatga ctggatctaa aaaattagca aaaagtgatt acactaaaac agctttacgt
     7201 gccttttatt tacaaaatgg ctttaattac agtaactatc aaggtcttgg ttatgctaat
     7261 gtcatatatc cagctctcaa aaaatattat ggagatgaca aaaaagcttt agcgggagct
     7321 ctagaagaaa atgtggagtt ttacaataca aaccttcatt tcttaccttt tgtaactagt
     7381 ttacatttag caatgcttga taatgagcgg ccagaagaag aaattcgcgg tattaagatg
     7441 gcgcttatgg gtcctcttgc aggtatcggt gattctttat cacaattttg cttagcaccc
     7501 ttgttctcaa cgattgcagc ctctttagcg acagatggtt tggtgatggg accgattctg
     7561 ttctttgtgg ctatgaatac tattttaacg ggaattaaat tagttactgg catgtatggt
     7621 tatcgtcttg ggaccagttt tattgataaa ttgagtgaac aaatgtcagt tatctcacgg
     7681 gcagctaata tcgttggtgt aacggtcatc tcaagtttag ctgcaacaca agttaagctt
     7741 acaatccctt acacttttgc tccagaaaaa gtaactagta cagcgcaaaa aattgttact
     7801 gtccaaggta tgcttgataa aattgcacca gctctacttc cagcactcta cactttcctt
     7861 atgttttacc ttattaaaaa taaaaaatgg acaacttata agctagttat ccttacggtt
     7921 attattggta tccttggtag ctggttaggt attttagctt aataaaattt tgataaataa
     7981 ggattatgat atatcaatga catagtcctt tttatatcgg acgaggacgt tttgtaaaaa
     8041 aatgaaagat cgcttatata ataaattcaa ggattttgat agggaatttt gtcaaaagta
     8101 tattaaaaca taccaatcta atgcctatca agaaatgaaa gcctctgtta atttgatgat
     8161 gaggaacact tttgtattta atgataattg ggatatggaa ccttgttcta aagcatattg
     8221 tcttgatcct ttggagtggg ataatccagt aacagatgat ccagaatggt tgtatatgct
     8281 aaatcgtcaa acttatcttt ttaaattcct ggtggtctac attgttgaag gcgataagtc
     8341 ttatcttaga caaatgaaat attttatgta ccattggatt gattgccaat ttacactaaa
     8401 gccagaaggg gcggtaagtc gtactattga tactgggata cgttgcatgt catggctcaa
     8461 agttttgatt tttttagatt attttggatt aataacagaa actaaaaaaa ttaaactatt
     8521 aactagttta cgggagcaga taacttatat gagggactat tatcgtgaaa aagatagtct
     8581 aagtaactgg ggaattttac aaacaacagc aatattggcg tgtttatact attatgaaga
     8641 tgaattaaac ctacctgaaa ttcagagttt tgctgaagaa gaattattac ttcaaatcaa
     8701 gcttcaaatt ttagatgacg ggagccagta tgaacaatca attatgtatc atgtagaagt
     8761 cttgaagtcc ttaatggaac tagttatcct tgcgcctaaa tattatctac cattagaaga
     8821 aactattgaa aaaatggtta cctatctaat tgctatgact ggtccggatt attgtcaact
     8881 agctataggg gacagtgatg tgaccgatac tcgtgatatt ctaactttgg caacacttgt
     8941 gttgaaatct tctaaaacaa aatcattttc ttttgataat gttaatttag aaactttact
     9001 tttgtttggg aagccatcaa tttatctttt tgaagaaata ccgcgtgcaa caataggtga
     9061 gtctgcttat ctttttccag attctggtca tgtgtgttta cgtgatgata ggcgttatat
     9121 attttttaag aacggtccat ttggtagcgc tcatactcat agtgataata atagtgtttg
     9181 tctctatgat aaaaagaaac ctattttcat tgatgcagga agatatactt acaaagaaga
     9241 acaactaagg tatgatttta aacgttcgac tagtcattca acatgtaccc ttgatgggca
     9301 atctttagaa atgatcaagg actcttggac atacaattct tatccaaaat gtgactattg
     9361 tcagctgact tcaaaggata ggtaccattt agtcgaagga caactacatg tccaaagagc
     9421 ttctgatatc tattaccata agcgatggtt gttaacttta ccgcaggcca ttaccttagt
     9481 tattgataag gtgaattgtc caggagagca tgttttaaca aatcaatata ttttagatga
     9541 tcaggtcatt tatgaaaatg ggcttgttaa cgacttgaaa ttagtaagtc ctacgacctt
     9601 taatctagaa gattgcctta tttctaagcg gtataatcaa ttgacagaaa gtcataaatt
     9661 agttaagaaa ataaaatttg ttgatgaggt gatggattat accttgatag ttgatcggaa
     9721 ctgtcaggtg aaatgtgttc ctttagtgca aacgaacagt cataaggaac taagtaatag
     9781 cattgcattt gatattaggt ctcaagactt tcattattta attggagtgc atatggatga
     9841 tattatcttt ggggataaac tctatttgat gcaagggata aaatgcaaag gaaaagtcat
     9901 tgtatatgat aaaaataatg gcaaaatgag tcgtttaaaa aattaagagg gctattatta
     9961 tgctatacta gtactatatt aaccataaaa aaataaaatc agtaaaatta gaaaggaggt
    10021 ttgttatgtc taaaaaaatg accattaatg acatcgctca attatcaaaa acttccaaaa
    10081 caacggtttc ttttttcttg aatcaaaaat ttgaaaaaat gtcggatgag acgaggcagc
    10141 gtattcaaga ggtgattgat gaaacaggtt atagaccaag tacgatagca agaagtctaa
    10201 attctaaaaa aacaaaacta ctaggtgtct taattggaga tataacaaac acattttcaa
    10261 atcaaattgt aaaaggaatt gagcacatca ccaaacaaaa aggctatcaa attattgtgg
    10321 gtaatagtaa ttatgatgcc aaaagtgaag aagattatat tgaaaatatg ttaaatttag
    10381 gtgttgatgg tttcattatt cagcctacat caaattttcg aaagtattct cgtattttaa
    10441 aagaaaaaaa gaaaccaatg gtattttttg atagtcaact ttatgaacat aagacgagtt
    10501 gggttaaggc taataactat gatgctgttt atgatatgac acaggagtgt ctaaatcgag
    10561 ggtataaaaa gtttattatg attactgctg atacgagctt gcttagtaca cgtattgaga
    10621 gagcttcggg attcatggat gctttaaagg acaatggttt cggttatgac actttggtga
    10681 ttgaagatga tgatcattca aaaagtgata tagaagactt tctaaaagcg gtagttccag
    10741 ataaggagga gacattagta tttgcaccaa attgttgggc tttaccaatg gtttttactg
    10801 ccatgaaaaa tctcaatttt gatatgccac gagttggttt agttgggttt gacaatattg
    10861 aatggacaga cttttcatct cctaaagtgt caacaattgt ccagccggct tatgaagaag
    10921 gtgaacaagt agcacagatt ttgattaatc gtattgaagg agatgactcc gtcgataacc
    10981 aacagattgt agattgccag atgttttgga aagagtcgac tttttaaaaa aagatttctt
    11041 attttatttg ctattaaaaa ataaaaccta gagaaaatta tctctaggtt atttgtcctt
    11101 attctaaaat aagtaagcct tctttgacag cgaaaggatt tttctcattg atacggtcgt
    11161 aaaacataat accatcaatg tggtcaattt catgttgaac aacaatagag ttgtaacctt
    11221 ttaatttgag tcgatgtttt tcaccagttt tgtcgaaata ttcaatagtt acacgcgcgt
    11281 ggcggacgac atagccaggt acttcacgat ctactgatag gcatccctcc ccgtcagata
    11341 aagcagcatc ttgaacagaa tgcgatacaa ctttagggtt gtacattact tcttggaggc
    11401 tataagcttc tttaggtgga tttccttgag catcctcaac gtttggaaca aggacggcaa
    11461 tgatgcgttt tgagatgtct aattgtggtg cggctaaacc aacaccacca cgtaagccta
    11521 gcttctcagc cataattgga tcttgagagt gttttaaaaa ttgcatcatt ttttctccga
    11581 gaatttcttc tttctcagaa agtgggaatg tgacttcttc agccactttg cgaagggttg
    11641 gattgccttc gcggataatg tcattcatgt caattaaatg actagctttt actaatttat
    11701 caatagcaga catattttct cctaactgta ttctctgatt tagtttagca taaaataatt
    11761 aaatgataaa gctacgcttc atggctgaat atttaatgat tctagtgcga aacgattttt
    11821 gataaagtat tttggttttt ctttttggat aaggccttct ttgaccatat catggataac
    11881 tcttaataaa tgacgataac tgatgccaaa cgaatcagct aaactactta gttcaaggtg
    11941 aaagcaacct tgttcttcaa ttgctaaaat atgacttgca agtaactctt tgacggtata
    12001 ccctaaatta gtagacgctt tgatgttttg atcatgaaaa gcctgagcta gctcttgact
    12061 aagttttagg aggaaagttg cgtctgttaa cagtttctct ttccgtcctt taagtgataa
    12121 ttgaatgact gtaatatcct ctaaggcaat aacagatgat acaatttgtc tattagtcaa
    12181 aagttcaata tcaccaatca gtgttggttt tgttttaata tctaaaatat gttctttgcc
    12241 gttaaagagt cgacgtacaa ttttaaaacg tcccttgacg atgtagtgca agtattctaa
    12301 ttgttctccc tgtttacaga cggattggcc ggcagttagg gtgattactt gtaaatcctt
    12361 gaaataatgt tttagaaaga tagtctccaa acagtagtct tggcagtagt tttctatatc
    12421 catgtattga ctccctattt gagatagttt taggacatat gtcatatatt tctagtttaa
    12481 tattgattat actttattta tctaaaaaaa gacatcagaa agaggtattg tcacttgaaa
    12541 atctttatgg agaagctaag tttactctct ttatcactta tcttgctatc aactttttcg
    12601 acatcaccag ccctaccaca aatgatatct tattatagag ataagggctt accatcaccc
    12661 caagttgaat tattattttc aattccttcg atggctatta tcttcatctt gcttatcaca
    12721 ccttggttaa gtaaaaaact ttctgagaaa catatgatta tctttggact attgctaaca
    12781 gcattagggg gaggattgcc ggttgtcagt cagaactacc ttttagtatt tgtatcgcgt
    12841 cttttattgg ggagtggtat cggttttatt aatgctaggg ccattagtgt gattagtgaa
    12901 tattatcaag gcaaagaaag aagaaaactc ttaggattaa gggggtcctt tgaagtttta
    12961 ggaaatgctg gtttgacagc cttagttggg ctattgttga cttttggttg gtctaaatca
    13021 tttatgattt attttttagc tttaccaatc ttagttctat atcttgtttt tgcgcctaag
    13081 aaagtggtga aagatacaaa tgacaagatt aaaactaaag gtcaaaaaat ccctaaagct
    13141 gatttgactt atattgtcgc cttagctatt ttagcaggat ttgtcataac tataaatact
    13201 gggattaatc ttaggatacc tctgctagta gtagaatttg gtttgggtac gccagctcaa
    13261 gctagtcttg ttcttagtgc gatgatgctt atgggaataa tagcaggtat gagttttggt
    13321 cagttgatag ctatgtttca taaacaatta attccaatct gcctcgtctt attttcttta
    13381 acattgcttg gagttggatt gccttctaat ctaatggtac taacaattag tgcgatggcg
    13441 tcaggtttcc tttacagcct gatggtcact gctgtgttta gcctagtagc agaccgtgtt
    13501 gaatattcgt tggttggttc tgcaacgaca ttagttttag tattttgtaa tattggtgga
    13561 gctagtgcgg ctattcttct gtcttgtttt gatcatttac taggacaaat aaatgcagtt
    13621 ttctatgttt atgctatttt aagtttagca gttggtatga tttatttcct taaacgccta
    13681 ttttttaaga aaatgtagtg tatattaact aaaaagggct tgacaatcat tgtcaagctc
    13741 tcttcatttt tcttcttctg ggtaggtaaa tccccattct tgacgtagct gtgtcatgat
    13801 atccataaca tctttggtat agtttaagta catgtggtta gtttttcctg aaatggcttc
    13861 ttccatatca gctacctcat attggagagc attttcagtt ttgccagctt cgataatatc
    13921 ttgatgccca tcctcagtat aagtaataac tgccttttgt cctcgcggat attcaaaaag
    13981 ttcaatgtag cctttatcgt aagcgatagt tgctcgttta ggttgttttg catgtaaact
    14041 aaggctgaca gtcgccatct catttgctgg gttggttagt aggataccaa cttgttcatc
    14101 aacccctgtt ggtgcaaatg taacttgaga ggtaatgttg tgaggtgctt ctgacataaa
    14161 ccagcgaata caagaaagtg cataaacacc aatgtccagc aaagcaccgc ctgctaggtc
    14221 acgactgaaa aaacggttag tcatatcata ttctttataa cttccgaaat tcatttgaat
    14281 catttttaac ggtcctaatt ttccactatc aactaatgtt tttaattggc ggtaaattgg
    14341 catatgaaaa atagtcatgg cttcagctaa gacaacatgg ttagtttcgg ctaaatctat
    14401 ggcttcttta agctcagtac tatttaaagt aatagatttt tcgcaaagaa cgtgcttacc
    14461 atttgctaaa gcctttcgta aaaatgagat gtgagtattg tggggagtag agatataaat
    14521 gatatccact tcagggtctt caaatacttg atctatgtga tcataaactt tttggatacc
    14581 atatttgtta gcaaattcaa gtcctttgtc gtaagttcta ttagctacag aatataattt
    14641 ttgtccacgt gcttccagtg cttgggctaa ttcgttggca ataacgccgg ttccaagcgt
    14701 tgcccaatta taatattttt cagtcataac aacctccttg ttggtttata gtatagcaaa
    14761 aataacaaaa tgatatttaa agaaagcacc aatatgagag tatttttaat agtcaaactc
    14821 tgaattttct gttagactgt aagagattac atatgagata ttaaggagaa aataatgata
    14881 aaatatcaag atgattttta ccaagcggtt aatggggaat gggctaagac agctgttatt
    14941 cctgatgaca aaccaaggac aggtggtttt tctgatcttg ctgatgacat tgaagcatta
    15001 atgctgtcaa cgacagataa gtggctggct gatgaaaata aaccaagtga tacgatttta
    15061 aatcatttta ttgcttttca taaaatgaca gctgattatc aaaaacgtga agaggtagga
    15121 gtatcaccag tattaccatt aattgaagaa tataaaggtc tacaatcttt ctctgagttt
    15181 gcatcaaaag ttgcagaata tgaactagaa ggtaaaccaa atgaatttcc atttggtgtg
    15241 gctcctgact ttatgaatgc tcaattgaat gtactttggg ctgaagcgcc tgggattatt
    15301 ttaccggata caacttatta tagtgaagat aatgaaaaag gaaaggaact ccttgctctt
    15361 tggcgtaaaa gtcaagaaga cttactgcct ttgtttggcc tttcagaaca ggaaattaaa
    15421 gatatcttgg ataaggtttt agctttggat gcaaaattgg cacaatatgt cttatctcgc
    15481 gaagagagtt cggaatatgt taaactttac catccttata actgggaaga tttcacaaaa
    15541 ttagcacctg aacttccatt agatgctatt ttccaaaaga tattaggaca aaaaccagat
    15601 aaagttattg ttcctgaaga acgtttctgg acagaatttg catctgacta ttactcagaa
    15661 tctaattggg aattattaaa ggcagatctt attttatcag ctgcgaatgc ctataatgct
    15721 tacttaactg atgatattcg tatcaaatcg ggagtttaca gtcgagcttt atcaggcaca
    15781 ccacaagcaa tggataagaa aaaagcagct tactaccttg cttcaggacc ttacaatcaa
    15841 gctctaggtt tgtggtatgc aggggaaaaa ttctctccag aagctaaagc tgatgttgag
    15901 cataagattg caacgatgat tgatgtctat aagtcacgct tagaaaaggc ggattggctt
    15961 gcacagtcaa cacgggaaaa agctattgtg aaacttaatg ttatcacgcc acacattgga
    16021 taccctgaga aactaccaga gacttatact aaaaaaatca ttgatcctaa actatcgctt
    16081 gtcgaaaatg ctactaattt agacaaaatt tcaatagcct atggttggag taaatggaat
    16141 aaaccagttg atagaagtga atggcatatg cctgcccaca tggtcaatgc gtattatgat
    16201 ccacaacaaa accaaattgt cttcccagca gccattttac aagcaccatt ttatgcttta
    16261 gagcaaagtt catcagcaaa ttatggggga attggtgcag tgattgcaca tgaaatatca
    16321 cacgcttttg acactaatgg agcttcattt gatgaacatg gaagtttgaa taattggtgg
    16381 actgatgaag attttaaagc tttcaaaaaa cttacagata aagtagttga gcagtttgat
    16441 ggactggaat cttatggcgc aaaagtcaat ggtaaactaa cagtatcaga aaatgttgca
    16501 gatttaggtg gagttgcttg tgctttagaa gctgcgcagc gtgagtcaga tttttcagcg
    16561 agggatttct ttattaactt tgcgacaatt tggcgtatga aggcacgtga tgagtatatg
    16621 caaatgttag ctagtgtaga tgttcacgcc ccagcacaat ggcgaactaa tattacagta
    16681 acgaattttg aagaattcca taaagagttt gatgttaaag atggtgataa catgtggcgt
    16741 ccagttgaaa aacgtgttat tatttggtaa ctctaaatga taaactcccg ttactcagat
    16801 gagtagcagg agtatttttt tattctttta tagataaaga taatgtagta gtgtcaacgc
    16861 ttatctcagc tccaatagtc actgtcaaaa gaggttgggt gtgcgcaaaa tccatatcat
    16921 aaataacagg aatttccttg aaaatagcat gttttgaaag gatatattca aagacttgtg
    16981 gagtcatgcc gcattcttta ggaaagcgcc ccataagaat agcttgtgga tgaggataag
    17041 cttgtaaaat tgcagcaagg ttgcggtcaa attcataaaa attactttcc tcagcctctt
    17101 caatcaagag gacatagcgt tcaattttcg gtgcataagg ggtacctcga agtaggccaa
    17161 acgtagagag attacctccg ataatcgtac cggaagcttt tccgtgatta taaattttcc
    17221 attctgttgg gaaaaattgg cgtggtttgc tgggatcata ccagggatca ctagaccatt
    17281 cttcactagg ccaaagttca tagtggtttt ctgtcatggc agtaagccaa gcttgtgttt
    17341 ggtaaggttg accttccttc attttgaaag aagaataagc aggacccatg taagtttgga
    17401 tttttgcttt ggcaaaaata gcattaagga aggcggttga atctgagtaa ccacaaatta
    17461 ttttgggatt cttagagatt aaatcataat ctaaatatgg taaaagctcg ttggaattga
    17521 atccgccaat ggttgctaat atggcatcaa cagaagggtc ggcaaatgct tcatgtaaat
    17581 cttctacgcg gctagcaatc gaagcagaat agaggatatc attttctaga tagtgcttag
    17641 agaatgacac ttgaaatccc agcttttcta gtacttcttt ggctaatagg ttagcctcaa
    17701 aaccacccag acgttcaatg gaagaagagg gactaagaac acgaatgtgg ctgccttttg
    17761 ttaatttttt cattggttaa cctcctattt aaattgttgt tagtctacca catatggaga
    17821 taatgagcaa cgatattctt aaattctatc aatttaagaa atgcgtgtta tactagagat
    17881 agccgatgaa agaaaaaata cttattatta ataatttggt tattaaatag gaggtcatga
    17941 tgtctcaatt atctttggaa gaacgattga atcgactaaa agataaatac ctttcttcta
    18001 aaaatcaaga attttcaaat gttcacttaa gtaaagtaaa gaagaaaaag aagaaattaa
    18061 taatacttga aaaagaaaga tgtcgctgtc gattggagag agaagatact aaaaaaatcg
    18121 atgataaaat tgagaaattg aagaaagaat tgatagatgt tatgaaggag gaataatatg
    18181 gaagaaacta ttctaatagt cagctttctc ctgtttttga ttttatctaa tgttatcaat
    18241 aggatatttc ctaaattacc attaccattt atccagttgg tctttgggat tctctctggc
    18301 ttagtttttc ataagtcaca agtccatatc gatcctgagt tatttcttgc ctttgttatt
    18361 gctcctttga actttaggga aggtcaagaa agtgatattg gtagttttgt aaagtatcgg
    18421 actattattc tttacttaat cttaccaacg gtatttttaa cagctattgt cgttggttat
    18481 gtagcagggc accttttgcc agttagttta ccacttgcgg catgctttgc gcttggagca
    18541 gccttaggcc caacagatgc cgttgccttt atatcgattg ctaagaggtt tcaatttcct
    18601 aaacgagcag agaatatcct caaattggaa ggtcttttaa atgacgctag tggtctagtt
    18661 tccttccagt ttgctttaac agcccttgta acaggttatt tctccttagc aaaagctagt
    18721 ctcaagttag cattggccat aatgggtggt tttttaattg gcttgctttt tgcttttcta
    18781 atgaggttat gtctcactgt cctagagaaa tttgatgctg ctgatgttac aggggctcta
    18841 cttttggaat taaccttacc ttttgttgct tatttcgttg ctgatttgtt gggattttca
    18901 gctattattg cggtggtagt ggcaggagta atgcaagcaa atcgtttgaa aaaagtgact
    18961 ctctttgatg ctcaagtaga tcgcgttacc tctgttattt gggaaacctt aaattttatt
    19021 ttaaatggtt tagttttctt aatatttgga agagaactga tacgtatcat tggtcctctt
    19081 ttaacaagta acgcttatag taattttgat cttattagta ttgttgtttt agttacttgc
    19141 acactttttc tagttcgctt tctagcagtt tctcgctttt atgcttggag atctttcaaa
    19201 taccataaat catttaaaaa gtattggaga gagattcaac tgttgacttt ttcaggagtt
    19261 aaaggaagtg tttccatagc aacaatactc ttattaccca agcactcagt cataggagag
    19321 ttggggtata gtttgattct tttcactgtt ggtgcagtaa cgttaatgag ttttttaacg
    19381 ggacttttag tattaccaaa gttagcgcct cccttacagg tgaaagatga ttatttaata
    19441 cgtctatcga ttttaacaaa agtattatct gtcttagaag aagacggtaa gagttctgaa
    19501 aatcaagctt cattttatgc agttatagat aattataata gccgtattcg tcatctcatt
    19561 ttagaacaag aatctagtga cattaagaag gacttagctg aattgcaact gatgatgctt
    19621 agtatagaaa gcgacgggct tgaagctgct tatcgttatg gcaatatttc tatcaaagaa
    19681 tatcgtattt accagcgtta tttaaaatac ttagaacgtc aagttaatcg tagctttgtg
    19741 tcaaatatca cttatagttt aacgattctt atgcgtgtta ttcgtggtat tgttcatgag
    19801 tttttaacat ttggacaagg aatacgaaat cggaaaaaac atttcaaaaa caggaaacgg
    19861 ttgttagcag aagtcaatcg tcagcactta acagatttgt acttatcaaa taatgacctc
    19921 attttagaaa cgctagaaga tatggaaggt tattataatg caactttgat agatttctta
    19981 aaaaatgaac gcattcgaga cgcagaatta attaaatcag gtttatttgt agaacgtgtc
    20041 attgcacgca taacgccaga tattttaaat gaaaccctaa gaggatatta cttggaacgc
    20101 agaacaatct ctgaatttga aagggacggt ttaattagtt ctaaagagtc tcaaatttta
    20161 agggataatg tcaatgaatt agaaagctat tcattaagag atagtcaatc ttctatacct
    20221 tatcaattat tggatttaaa aaaataattc tgataacaaa aaaatcaacg aacaatcgtt
    20281 gatttttttg ttatctttgg ataaaatctt ttatatttaa ggcggtagac ggatttgaaa
    20341 accttggttt atcaaggttt ttggttgttt cgccccaaat tcgccccaaa ttatgcgact
    20401 aaaaaaatat ttttgatttt ctcaaaattt ctctcagcca gtgcttccat ctgatgtgta
    20461 taaactttca gagtaatatc tggactttca tgtccaagga gcttagaaat tgtcacaatg
    20521 tcaaccccgt tcaaaatcaa aaacgaagcg tatgtgtgcc gtaatgagtg atttctgact
    20581 ttcctaccca caattttctt aatcagttta ttgcaagctg aattagatac accaaagcac
    20641 actctattct tgatattagc ttgccaatga tttttcttat attctcgaag aatatctatt
    20701 gtatttgaat caattggaac ttttcttttt gatgactcat tcttcagatc tgcaaaatct
    20761 tgagtattcg agtaatcaaa actcttgttt atatccaaaa tgccattttt gaagtctata
    20821 tcgctccagg ttagtcccat gacctctgag aaccgcaaac cagtgacaga aagaaggtag
    20881 agagtgaaat aggatacata ctggatattc tctcttgtga gggataaaag agccttatac
    20941 tcactttctt ctaagaagtc attttcctca gctcttgatt caatttgtga tttaaccttt
    21001 gcatcatcag caaagttaaa atgaataact tgttccctca cagctacttt catagctccc
    21061 ttgatttggt agtggaactt ctcaagagtt tcttgagcat atttctcccc aaattcattt
    21121 agccgttttt gataatagag tggtgttata tccttaacct ttatatcttt aaaataggtt
    21181 ttgatgtgtc taaagttctt tgtgtaggtt tcccaggtct tatcctttac ataaggacgt
    21241 ttataaactt ctgaccaagt tttgacaaaa tcatatagag taacttctcc atcggtcagg
    21301 atattttgag atagtttttt ctcaatatcc ctagcagctg cttgagcaag ttttttggtt
    21361 ttgaaaccac tcttagactt ttgacggaac ttaccgctct catctttata tgaaatgcga
    21421 tattcccagc cattatcacg ttttcgataa taagccattt gtttttcacc tcattttttg
    21481 ataaaatggg tatagtaaag agacctactg ctaaagcagg tttctcacta tccacaattg
    21541 ccttacgctt gcaccgacca aagttgagcg tagggctttt tttatttttt aactttattt
    21601 ttcaacgcat tctcgatagc atttttaagc tcaagaatag ctattttctc atctttactg
    21661 aaggttactg tattttcgtc ttttactgca tcgaaaacac cgcccttagt atcagatgaa
    21721 ccaggataaa ccaattgtag atagccaaca gttgaactag gttccttgag ttgataagct
    21781 gtaatttctg ataatagaat tgacttttct ccatctaagc catgtagcaa catatttgac
    21841 atgttgcttt ttcttgcgat tctgatgaag taatcatcaa tacgtacaat tgtttttgat
    21901 tttttgaatt caaaaacacg ttcgttaggc tctgatgtaa agagttctac ttcaatttct
    21961 ttatcttttc cgccaaataa tgccattatt gttgtctcct tttgttttta ttgtaaccgt
    22021 aaaatgttaa aaatctgccc tccaccttga cggtagttta aaactaactt gagggcgtag
    22081 ctttcagcat actgcaagtt atagatgaga tagttcatca gtctgttgtg tagtgctggt
    22141 ttgctaatat ctgtttcacg ttgaatatct tcaaagctct ctctagttgt gagggctttt
    22201 ttgagtcgtt catctgttag gtagaggatt gaagccactg tatcagcttc tttctcaatt
    22261 ggaatcagct ctttcgggta cttctcacta gcgtttttac tcatgagaga catatagatt
    22321 ggctgatttt ctgtttgatt aaggtgacag tgtatgtgac taagttcgtg taaaatcgtg
    22381 aagataacac gtcctttggt gtttgtatgt tgattgatat agatgatgta gcgtccaagg
    22441 tctaggtcag ggacagtcaa tccagcacag ctttcgcaga gtaccctatc agtataagta
    22501 acggttctat ttgataccag ccctctgtac ttgatgtctt tatctgtggg atgataatca
    22561 ggtaattctg gataacgttc tttcatctca tcgtaatcaa gaaagttaaa gagaatagga
    22621 tagttttgtt caaaatgttt aatgacatct tgataactaa cgttctcttt cattttatca
    22681 attgagctta ttatttgata agccttattg tgatagtcaa aatactgttc ccttgatagc
    22741 ggtgtgtatt tcaataagtc acctacttcc agttagaatc atcttgaatc aattgtctag
    22801 ctgtcttcat tagaccttca agagcagaat tgaaccgctc tttttcagaa gctgaaagtc
    22861 cttctgtttc cttacgaaaa agaacaatag cttgctgagt tagatcatca actgcagtat
    22921 tatcttggcc atcggcaatc ttaggatttt ctgttctacc gagaaggtag tcagtgctga
    22981 cattgaaata atctgctatt tttgagacac gctcaacatt tggtgtagat tttttcatgt
    23041 tgtatatggt atttctagga aaatccaact gttcttcaag ttgattgagt gaaatacctt
    23101 gcttgtcagc caattccttg atcttttcaa atgtaaaaaa cattgattta tcaacctttc
    23161 taaggcatga caaaaaatat ttaataaatt tactacaaaa ctgttgacag gttttaataa
    23221 atttactaca atatcgtttg taagctaagt cgttagcgaa caagacaagt aaaaaataaa
    23281 gcctaaacaa ctgattggcg tcggttttat gggttaagcc ttagcttttt agcaggtctt
    23341 ttctctatgc tttgatttta ataaatttat ttattgttgt caagaagttc gctaactttt
    23401 tagcaaaaat aataaaagga gggtagatga atgggaatcg gttcaatgac agtgacagtt
    23461 aaagttacta acatggataa atttattgaa cttagtaaag agttcaataa aaaagctcgc
    23521 gagctagaag aactagctca cgaactgaag tcatttgatt ttgaaggcga agttgtatca
    23581 actgatagca actagctcaa atgattcatc atctgaaaaa agataaacag ggtattcttc
    23641 tttagttgca aaattgaaga aatcatatcc aaaattatct tttttgatgt gggaattttt
    23701 ttcggagaga gagtcaagat tgacctgaga tagtagctta aagatttgca cacgagcttg
    23761 ttcagcatcg ttaactcttt gcaaggctct caagtgctct tcgctaggga aagctgctat
    23821 caaatcagct ctgtcatcta tgtaagttct agtgtcatca agatagccat attttagatt
    23881 atagtctaca aatgaccgct gttcatattc ttgataggtt tcaaagctga caaggttacc
    23941 tttgtaacga tagacgagaa cgtcatcttg taactcttct tcgaaatttg tgatgccaat
    24001 tagaaattta tggcatgcct ctatgatacg aatttgattc aaattcatgg cttgacctcc
    24061 tttcataaaa gatttcaagt caattatacc aaactagaaa ggaaaaatat gagtcaacaa
    24121 cagaaaaaat ggattcagct agtgaaagat aagctgaatg aagagcaaat gacacagaca
    24181 catctagccc gtgcttgtgg agtcgcaaaa gcgacaatct cagagctact aaaatacgga
    24241 aaaggtagtg tccgtctgaa aaataaagtc tcagacattc ttcacattga tgaaagctgg
    24301 acggacttgg aggaagattg atgccgataa caagagaaat gacaatcact gaaatcaatg
    24361 ttctcaatgc tatcaagaat agcgctactt atgatcttcc gattcaagcc agagagctaa
    24421 ggcagcagct aggacttagc aaacgatcac ttgaagcagt cattgagaac ctgagagtga
    24481 tttacaagca accaatcgtt gccaaaaaaa aacagcctag cggatactat cttccaagaa
    24541 atgaagaaga aagaaacgac ggcttagccc catacaaaag acagatattg actgagcaaa
    24601 ataatcttgc tgctattatg tctgtcaatc tggaagaata ttgggcaaca gaaaaagcct
    24661 gacggcaatc aggctcacaa aatattatta caagaggatt atatcacaat gaacgaagtt
    24721 tggaaagata taccaattgt taaaggatat tatcaagttt ccaatcttgg tcgtgttcgg
    24781 tctattggta gaacagtcaa tgcaaaacag agaacaagaa aaacaaaagg acgcatctta
    24841 aaacagagct tatcttcagg ctatgcaata gtgacacttt cagtcaatgg attaaggaag
    24901 tcgataagag ttcatagact agtagctgaa gcatttatac caaatccaat caataagcga
    24961 acgataaatc atattgatga aaataaactt aataatagag tagataattt agaatgggcg
    25021 acagataagg aaaatgctaa tcatgggaat agaacaacga aatcatcttt aggtcgttgt
    25081 aaacctgtag aacaatttac acttgaaggg gaattcataa acacgtttga ctcaataaaa
    25141 tctgcatcaa tgaaaacagg tatctcgtca caaagaataa cagcaacagc aatggggcac
    25201 caaaaacaga ctcacggata taaatggagg tacgcataat gaatgatcta atgattcaaa
    25261 tgcttgacca gttcgaagct ggtctaatgg acagaacgat gaaagtaatg cacgttgtga
    25321 cagacgaaaa gtatagattt cctttagaac tcaacaagtc tcaatgcgct ttaatgctct
    25381 taggtactaa agatactggg acatttgatg aacgctttaa ctgtcataag gattttccga
    25441 ggattccaaa tgctcgtgag aaataccctc gtgatgaagt cattgaatgg tatcacaaga
    25501 attggcaaag aactgttatc taagaaagaa caaaaggaac tatatcatga acaatttaca
    25561 aattatcgca gtaggcacac ttatttcagt aaccctgatt gagtctctta tctcaaatat
    25621 caagctcaga aaagctctca agaacaaaca gacagctggg actgtcattg ctcaatatat
    25681 cccacaacga ggcttcattg actacaagac tggaagacgt gttgacattg accctgaaac
    25741 aaagaaagaa gtctttgttg actgaggaaa gctatggatg aattaaaagt attgcctcac
    25801 gatatccagg ctgagcagtc ggttcttggt tcaattttta tcaaacctga aaaaatgatt
    25861 gaggtggctg agtatctgaa accaaatgac ttttacaggc cagctcacaa gattctattt
    25921 aaggccatgg tgagccttgc tgatcgtggc gaggccatcg atattgtcac aatcaagtca
    25981 acgctggaaa gtacggatga acttggcatg gttggcggta tcagctacat cgctgagatt
    26041 gttaatgcag tgccaaccag ctcacacgct gagcattatg ccaagattgt tgctaaaaaa
    26101 gcacagctac ggtctatcat tgacaacttg tctgactcaa ttggcaatgc ctatgatgag
    26161 gacatggata ttgatgagat tatcgcaaaa gctgagcgat cacttatcga agttagtcaa
    26221 gccagtaaca agagtagttt cagacctatc catgatgtcc ttttggaaaa tcattcaaag
    26281 attgaggaac gctcaaacaa taccagtcaa atcactggta ttgaaacagg attttatgac
    26341 tttgacaagc tgataacagg cttgcatgaa gaccagctaa ttgtcttagc ggcacggcca
    26401 gcgatgggga agacagccct agctcttaac atagctcaga atgtggcaac taagtccaat
    26461 aaggctgtgg ccgtcttctc gcttgaaatg ggggctgaaa gccttgttga gcggatgttg
    26521 tcagctgaag gaacaatcat caaccatcat attagaacag gaaatttgac ggttaatgag
    26581 tggcaacgtc ttatctatgc acagggtcag cttgctgaag ctccaatctt tatagatgac
    26641 acagctggag tcaagattac tgacatcaga gcaagagcta ggaggctatc tcaagagact
    26701 gacggacttg gattgatagt cattgactat cttcaactca ttcaagggtc acggtctgac
    26761 aaccgacagc aagaggtatc tgagatttct aggcagttaa agattattgc caaggaatta
    26821 aaagtgccag tcattgcact atcacagctt agccgtgggg ttgagcaaag aaacgacaaa
    26881 aggcctatca tgtccgactt aagggaatca ggcagcattg agcaagacgc tgatattgtc
    26941 gccttcctct atcgtgatgc ttattaccaa gacaaaaaag aaggtcagcc agaaaacgac
    27001 atcactgagc tgatcatcag aaagaacaga cacggaaacc ttggcacagt caagctgtat
    27061 ttccacaagg aatataccaa gttttctagc gttgaggagg aatgaaatga aaaaatttga
    27121 ccactatttc agcaaccata ttgctgtaaa aaacgaatct gatacagtca ccattgttga
    27181 ctattacaat ccagaaaagg gctatgagta taacttacgt tacattttcg atagaaaaaa
    27241 ttcatccctt gctatcacag gagactttgg ggaattagtt gcacgaaatt tctacaatat
    27301 gggtgaatgg gacacattct acaatcacta tactggtcat ctggggtatt ttcttgaaaa
    27361 gttactatgt tcaagtaggc cagttcatta ctatgatact gacaaggcta aaagagaaat
    27421 tctcaatgaa ttctttgatg gtgctgatta tgatgattta gattttgatg atgaattcct
    27481 acttgatcaa ttatttgaca attttgacga agataaagga tttacttata ttcttccaga
    27541 ggcttttgaa cgttttagcg aagatatgga agaatatgag gtctatgaat ttctatcaag
    27601 ggctggccga tacgtcaatg atgtctttga actttactta gacgcttata agagagctta
    27661 tgagtatcta acaaaggagg ttgatgatgg cacaacgcag aatgtttagt agaaaaatca
    27721 ctgagactga ccgttttttg gaaatgccac tatcatccca agctctctat ttccatttga
    27781 acatgggagc tgatgatgag ggctttatag ataaggcaaa gacgattcag agaactattg
    27841 gcgctagtga tgatgatatg aaattactga ttgcaaaagg gttcttgatt ccctttgata
    27901 gtggtgtagt cgttatccgt cattggcgga ttcacaacta tatccagtct gaccgctttc
    27961 aatcaacctt ataccagtca gaaaaggctc agctagagta tgacaagtca aaaacatcta
    28021 gtcttaaacc tattggaaat tgtatacaaa atgtatccaa aatggagaca caggttaggt
    28081 taagtaaggg tagcttagat aaggatagct taactaccta tcctacagtt tcagacaatg
    28141 aggaagaaga tattccatac aaggaaatta tttcttactt gaatgaaaaa gctaatagaa
    28201 attacagacc taacattcag aaaaacaaaa ctcttatcaa ggctagatgg tcagaaggat
    28261 tcaggttaga tgatttcaaa catgtgattg ataccacggt caaggattgg tcaggcacta
    28321 agtatgaaaa gtatctcaga cctgaaacgc tctttggttc taagtttgaa ggctatttga
    28381 atcaagcacc acgaatcaag acggaaacaa ttgacggaag gttgggcttt tagatgaatc
    28441 cttttaagaa ttttgaaact aggcaagtct tggaagaaac ctgcgaggtt cacggttgcc
    28501 agctctggct gactaaagta ccgattaagg gacggcttga agaactcaag cagtgtccag
    28561 agtgtaccaa ggccgctatc aacatctttg aaaataagtt gaacagtcag agcaaaatca
    28621 acagcaagct tgctgataca tatgctgtct ttgaaagaga tagcttggta tcagacaagc
    28681 taagaggcaa gagccttgag aactatgaaa tcaaggatga tattgaccag caagctatca
    28741 attatgctaa gcgtatggaa cagttctacc gacaggatag aacaggcaat gccattgtaa
    28801 ctggcccttc tggtgttgga aaaagtcatc tgacttacgg cttggccaag tttatgaacg
    28861 aacaattcaa agcctacgaa tcaccaaaat cagtcctctt catctcactg gtgagccttt
    28921 ttaccaaaat cagagaaagc ttcaaagctg acaatgggta ccgacaggct gagatgattg
    28981 agctgctaac tagtgtggac tacctctttc tggacgatct aggcaaggag agtcgcaaag
    29041 gcgacagcca gaacaatgag tggactcatc agattctcta tgagattttg gacaatcgga
    29101 gtaacacaat catcaacacg aatctgagca gtaaggagat taaggcctta tatgctgaca
    29161 attacggtaa tggtgctctt tctagtcgga ttcttgaggg agtgactggc aatagctttg
    29221 cttaccctaa ggatatggaa gataggaggt attgatgagc tataaagcca cattcatgct
    29281 atatggttac aaagctgttc cgattggtct atttgataca aaggagttgg ccgctcaagc
    29341 aattgttgac cacatcattg aaaactctgg aatacctagc cccagggtag ttgccactat
    29401 gaaaatccga agcaaggaaa tcagaattga ttatggttct aaaacttgct actacttgat
    29461 cactttaatt aataataaaa cggaggaaaa acaacatgac taaaacaatg acacttgaag
    29521 aaaaggttga acagtggttt attgaccgta acttacatga ggccaatcct gttaagcagt
    29581 ttcagaagat gatggaggaa actggggagc tttactctgg tattgctaag gacaagagtg
    29641 aaattatcta tgattcgctt ggagatatgc aagttgtcct gattggcatt gaacaacaaa
    29701 tcaagaatgg cgctcagatt gaagcaagtc cacaagatat ggagttgttg ctattggctt
    29761 caagtttggg ggaaatggca cagaaactct acaagcatat cttccacaat gaaacaaaaa
    29821 caccgcttat ccgtccagaa ttaagcttgc ttcactctac tattcatgca atagctatcc
    29881 acaacttgac aactgctgat gactgcctat caatcgctta tgaagaaatc aaagacagaa
    29941 aaggcaagtt gatcaatggc atttgggtta aggaggagga tttgaaatga aaaacatgaa
    30001 ttggaaagca ttcttcaaca atctcaaggc ctttgcaagt gtcatcatca ttactgtatt
    30061 tgttcttgga ttcaactatc tgttttatga gattggctac cagaaaggcc atcaggaagc
    30121 tgacagggtg attatctatg tcgctgataa tgctggcgct gagatgtttg gcaaaatcac
    30181 tgataaagaa atcattgaag gccgtcacac ggttacagct ggcgcttatg gaaaatttct
    30241 ggtcactgag gaacaataca atgaaatcac cgttggtgat gacataccag actacctgaa
    30301 aggacgtgga aactaatgac aaaatgtaat tttgaagcag gacacaaacc aattgacaca
    30361 attggacaac tagccaaaga agtccttgat gggaaatatg gcgatggtga ggaacgaaga
    30421 agaaatctag gctgtttata cgatctagtt caaacaagag ttaattatat ggttcatgaa
    30481 cgtgacagca agttcacagg tatcaagatt tacgacaatg tgaacaaacc tagccattat
    30541 caaggaaaga atggcatgga agccatcgat gttgtcagaa atttcattgg acagctcaca
    30601 ggagtatctg ctttctactg gggaaactca atgaagtacc tgttacgctt ccagggcaag
    30661 aacggccttg aagacctcaa aaaagcaaga aagaaccttg actggttgat tgaagaaatg
    30721 gagaaagaga aatgaaaaaa tcaataggaa tccttgaatt taaagaattt gatgcttttg
    30781 ttggtggaaa ggttatgcct gtctatcttc atagtgctaa cccaaacgtg ccaacgacta
    30841 ataaaaatga ggctatgagg gttaccaaaa agagatacag aaaagagttc tctgacaagt
    30901 acattttcag aaaggttggt gaggttgatg atcaataatg ttgtacttat tggccgcttg
    30961 acaagagatg ttgagctacg ttacacgccg tcaaacatcg caaacgctac ttttaaccta
    31021 gcagtcaatc gaaatttcaa gaatgctgct ggtgatcgtg aagctgattt catcaactgt
    31081 gtgatgtggc gacaacaagc tgaaaacttg gccaattgga cgaaaaaagg aatgctgatt
    31141 ggtattactg gacgaatcca gacaagaagc tacgaaaatc agcaaggtca gcgtatctat
    31201 gtgactgagg ttgttgctga cagcttccag attcttgaaa agcgtgataa ttcaacgaac
    31261 caggcaagca tggatgacca attgccacca tcatttggaa atagtcagcc tatggatatt
    31321 tcagatgatg atctaccgtt ttaggaggtg cttatgaacg aatataagaa acccacctac
    31381 atcgtcattc aagaggcaat ggctgagcgt attagatttc ttgaagatga attatataac
    31441 agagcataca aagacattga acggctagaa gttgagaatg accgtctgaa ggttagatgc
    31501 cttgatttgc agttggaaaa tgctgactat gtctgggatg acatgtgtcg ctctgctatt
    31561 gctcgtgcaa aatcagaaaa tcgaagacca cacagaagat ggaggggaag atgaagttaa
    31621 ggcttgaaga actaagaaac aaacttggcc tcagtcgcag agatgtgcat gaagccactg
    31681 gcatttctat gaacacgctg ctacattatg aaaaaggcgg tacaccatct attggtcagg
    31741 ttgaaaatat cgctaaggct tacaatatca acccagcctg gttgcttggt tggattgatg
    31801 aaggccagca accacaacca cttgaaaccg ttgttgaaaa gattatctat gtggaaaagg
    31861 aaggctgtag attaccgcca tattggaaca atgataataa tggcagaatc atcaagtgga
    31921 aagaatcaag aagagcattc caaaggagaa caatttgaca aagaaaacag caagtaagac
    31981 aagacgtgat ttccttgagt ttgagcttga ggctaagtat ctaaaaattg ataaacttat
    32041 tgggcagcgc cgtcatgaac tggaaagaat ctatgcagtc aaaaacctga caatgccagg
    32101 aatcgatgat tcaggagcta gtcgcagtgg aacttcaact aatacttctg aaaatctagc
    32161 tgttgcttat gccagtgacc caatgattct gaaactggaa gagttccaaa atgcgatatc
    32221 agaattacta gaggtacttg aacccgatga taagaaaatt ttccatctga ggtggggaga
    32281 acatactgga tatgattgga ttcaagtttg gcacatcatg gaaaatggcg agacaggtta
    32341 tctttacaga catagcaagc agatttatag aagacgtgaa gtcatccttg atacacttgc
    32401 aaaattattg ttcatgtaac ttgtcaaaaa aacatataga attgacagaa acaatctgat
    32461 agattgatag tgtcgctaag caccgagaaa tccttggtgc tttatttttt tgagaaagga
    32521 gcaaagcgat gaatattgtt gaaccgctac gagacaaaga cgatatacaa gccatgaagg
    32581 actatctatc atcttggaat gaaaagtatt acatgctatt tcttttgggc atcaatacag
    32641 gtttccgtgt tggagatatt ctcaagctaa aggtcaaaga tgtccaaggc tggcacatca
    32701 aagttaggga acaaaagact ggtaagtata aaagtattaa gatgacaagg ccactcaaaa
    32761 acgaattgag ggaatttgtc aaagacaagg aacttcatga gtatctattt cagagtcgtg
    32821 tcggaaagaa caaggcgctc agttataaga cggtttactg gtttcttaaa agagctgctg
    32881 aagacctcgg cattgacaat gttggaactc atacaatgcg aaaaaccttt ggctatcatt
    32941 actacaagaa gtacaagaac gttgctgact tgatgtcact attcaatcat tcaagcccag
    33001 cagttacact aatctacatt tgtgtaaggc aagatgagct tgatactaag atgagtaatt
    33061 ttagcctcta atattttttt gattttttca actatccata acgaggaagt ttctagtcta
    33121 tattttgaag tgagcttaaa gccttgtcct aattagtttt ctagtgtgaa acaaaattgg
    33181 ataaaatata agatatagat agttcagcat ggttatttta cataatttca gccatagaaa
    33241 aataatcttg tcaaaaaaat gggtattatt gacaaaaaca atctgatata tttgtaacat
    33301 gagaaaaatc cagagagcaa gcttaggcat gttctttttt ttgttggggg tgacgatgcc
    33361 atgaaagatg aaaatgcatt tgatggaatt aggcttgaag ctggtagcac tttagaaatc
    33421 tatctcacga agaacgactt agaacatatt gcaaatggat atgaggtgac tttagatatt
    33481 aagcctaacg agactgtaaa taaaatcgta ataaagccaa gttttgtaaa taatattcta
    33541 aatccgttaa tcaactatga caaaagaata gtgagtgaag cagatttaaa gtttagagac
    33601 atttcaagag aggttgcaag agatagtttt gcattggggt caatgtagtg ggctatagaa
    33661 accctaaaca ctctgactgg ttcagagctt ggcagattaa attctacaac tcgaaacctt
    33721 ggagaactct gagaaataaa atcagaaaag ctaagcgtat gcgttgtgac atgtgcggac
    33781 gtttgattca cggtaagagt attgttgacc acatcataga gattgacgaa actaattatc
    33841 aagatgaatc tatcactctc aacgaagaga actgtcagtt actttgtctt gaatgtcaca
    33901 acacaaaaac ttttcaaagt aaaataaatt taaatttaga taatcggaat ataaatttat
    33961 ttttgatttt tttatttttg aatttttgtg gactcccccc attttgaatt ttgacagcgc
    34021 caaaataata acggtgtcaa tcctcttgtg tacctctccc ccaaaattga cgaaaattga
    34081 tacaagaaag gagcatgatt ttgaaaatca atgaagtttt agaaaagcta ggaataagtc
    34141 gtgctaccct taccaggtat cgaaaaaagc tgggcatatt tgaagaaact aggtcaaata
    34201 tcaccaaaag tcagttcaaa gagttggaaa agcttgccaa tcaacggcaa aagtacacaa
    34261 ggcaggaacg tgttgagcta tctcgtaaga ctttcaagct gattccaaaa gaaaaaatgc
    34321 ttgaaatcag tgataatgat tcagttgtgt tgaaaaatct caaaactcaa tacaatcaca
    34381 atcaaaaagt gattgaaaac ttccagcttg aaatcaataa ggtcatcaat gacggtgagc
    34441 tacctgataa gtacctactt gatggaatgg aaaagtatca aaagcttaac atgcagatca
    34501 tgtcaacgat tgaaaagcaa agtccacagg gtgacagtct caaagaaatg attcaggaga
    34561 agttagcacg ttatggttga gatgagatat tttgataagt atgctcagct catctatact
    34621 ggtaagattc gtatttgtaa gctcacaatg aaatcaatta gacgtgttga gcgatacaaa
    34681 gagcaatacc tcttcaaaca ggaggaagct gacaaacgga ttgagttcat tgaggaagag
    34741 tgcagcaata ctaaaggcct tgctggtaag ttacgcttag cattaccaca aaaggtttgg
    34801 ttagaaacaa cgtggggctt ttatcacacg gttgaggtta ctaagaccaa tcctgatacc
    34861 ttggaagaat acacagatta tgaagaaagg cgtctcattc atgaggtgcc tattattgtg
    34921 cctcgtggca caggtaagac tactcttggt tctgctattg ctgaggttgg tcaaatcatt
    34981 gacggtgagt ggggtgctga tattcagctt cttgcttaca gtcgtgaaca ggctggctat
    35041 ttgttcaatg cctcaagggc gatgttgtcg aatgaagaaa gcttgctgca ctatatgcgt
    35101 gaggctgaca tcctacggtc aaccaagcaa ggtatcttgt atgaaacaac taacagtctt
    35161 atgtctatca agacttctga ctatgaaagc cttgacggta ctaatgctca ctacaatatc
    35221 tttgatgagg tgcacactta tgatgatgac ttcatcaagg ttgtgaatga tggttccagc
    35281 cgtaagcgta agaattggat aacctggtac atttccacaa atggaactaa gcgtgacaag
    35341 ctctttgata agtattacaa catctgggta gatatccttg atgacaagat tatcaatgat
    35401 tctgtcatgc cttggattta tcagttggac gatgtgtcag agattcatga ccctgatatg
    35461 tggcagaaag ctatgccatt acttggtatc acgacagaga aagaaaccat cgctcgtgat
    35521 attgagatga gcaagaatga tccagcacaa caagctgagc tgatggctaa gactttcaat
    35581 cttcctgtca acaactatct tgcttacttc agcaatgaag agtgtaaagg ttggtcagat
    35641 aagtttgatg agagtttgtt tgtcggagat gatgaacgga acgcccgttg tgtgattggg
    35701 attgacttgt cagatgtcaa tgacatctgc tctatctctt ttatggttgt gcgtggggaa
    35761 gaacggcact atctaaacaa gaaattcatg ccacggcata ccattgagac attgccaaag
    35821 gaactgcgtg ataagtacac ggagtgggaa ttaagtggca tgctgcatgt gcatgaattg
    35881 gactacaatg accaagccta tatctttgaa gagttacggc agtttatgag tgacaacaga
    35941 attttgcctg tggcagtcgg ttatgaccgc tacaatgcaa gggaacttat tcgcttgttt
    36001 aacgactact acggggatat ttgtcacgat attccccaga cggtcaaatc gttatcaaat
    36061 ccgctcaagg tttacaagga gaaggctaag atgggcaaaa tcatctttga tgatcctgtg
    36121 gcgacatgga atcatgccaa cgtccgtgtc aaaattgatg ccaataacaa tatttttcca
    36181 aacaaggaaa aggcaaaaga aaagattgac gtctttgcta gtcagctaga tgcctttatc
    36241 tgttatgaaa atttcaagga agacttgagc tactactttg attgaggtga agaatgaaca
    36301 actatttgaa aaatttgaag gaggtttttg ctaggatttt ccgtccaaac aatcggaaat
    36361 ctacaagaac ctatcttcaa agaagtatct cctactggcg taggaactcc atctatttgg
    36421 ataacatcta caataagatt tcaactgaca cagctcagtt aaggtttaag catgtcaaga
    36481 ttactcgtaa cccaggcggt gtcgattcga tggtttggta tgagcatagt gatttggctg
    36541 aggtgctaac agtttcacca aatccactag aagtgcctgt tgtcttttgg tcaaatgtaa
    36601 ctagggccat gctgcgtgac ggtgtggcgg ttgttgtacc acgttggaag aatggccgac
    36661 tggttgaaat atggcttgct aagaagacag tgacttggac agcagagagc gtggagctta
    36721 tgcttgatga tgttgctgtt gagcttcctc ttactgatgt gtgggtattt gaaaatccta
    36781 agttgaatgt cacggctcaa ctcaatcaga ttactgagct tattgacatt aacttgaatg
    36841 ctttgacaga aaagcttagc gatggcaatt ctagcttgag aggtttctta aagctaccaa
    36901 ctaaggcagc tgatgaacat ttgaagcaac aagccaggga ccgtgttgat agtatgcttg
    36961 acttggccaa gaacggtggc attgcttatc ttgagcaagg cgaagaattc caggaactta
    37021 gcaaagacta ttctactgct tctaaggaag aattagagtt cttgaaatca cagctttata
    37081 atgctcatgg tatcaatgag aaattattta cctgtgacta tactgaggaa caatatagag
    37141 cctactattc tagcgtcatg aagttatatc agcgtgtcta ttctgaggag attaacagaa
    37201 aatacttcac gaagacagca cggactcagg gcaataagtt actggtcttc tttgatatgg
    37261 ctgacatgat ttcatttaag gatttggttg aaggtggctt caagtccaaa tatgctggat
    37321 tgatgaactc aaatgagttc cgtgaaacct atcttggttt acctggctat gagggcggtg
    37381 aagtatttga aaccaacctg aatgctgttc gtatcggtgc agaagaatct gagtagaatc
    37441 taaagggtgg gtggttggca tatctttcac gaaaggaggt aagcaatgga aaaactaaaa
    37501 acctttgttg tgaagtcggt tgaggatgaa tcagctgact ttcattttga ggcttacgct
    37561 tcaacctatg acaatacaga cagagaaggc gatgtgatgg ccaaggggtg ttttgataac
    37621 actctgaaat ctaaggctgt tgtccctatg tgtcttaatc atgaccgtaa ctgtgttatt
    37681 ggtaagcatg aattgtctgt ggatgaaaaa ggcttgcgaa cacgttcaac gtttaatctt
    37741 agtgatccag aggctaagaa aacttatgac ctcatgaaaa tgggggcatt ggatagcctg
    37801 agtattgggt tctttattaa agattatgag cctattgacg ctaagcaacc ttatggcgga
    37861 tggattttca aggaagttga aatctttgaa atttctgttg tgaccgtgcc agctaaccct
    37921 caagcaactg ttgacaatat taaggaattt gatatgtcgg cggttgataa gcgaatcgct
    37981 caggtgaaca tgaggcaaga aatcatgagt aaacttgcaa aaatttaaag gagacatgat
    38041 gaaaaaatca ctcattgagc ttttggaagc tcgtcaaaaa gcaactgatg aactggctga
    38101 ggtaaagctt aaaaaagcca ctattgaagc aaagatgaaa tcttcaacca ttgaagacga
    38161 tgacttggaa cagttaaaaa ctgatgcaga aagtttggtt tctcaagcaa cagctattaa
    38221 ggaaacaatt gctgggctgg attctgacat tgaagaaact gaagaagaac tcagcaaagc
    38281 tgctaaaatc attaaagaaa aacagaaagg taatacacct atggattatt tgaaaaccaa
    38341 agccgctgct cttgactttg tccgtattct gatggacaac gaaggtagcg caaacagtgc
    38401 ccgtaaagcg tgggaagcta accttgttga aaaaggagtg actaacctca ctaagattct
    38461 tccagaacct gttcttatcg ctattcaaga tgcctttact aactataatg ggatcttgaa
    38521 ccacgtttca aaagatccac gttatgcggt gcgtgttgct cttcaaactc aagtatctca
    38581 agctaaagga cacaaagctg gtaagactaa gaaagatgaa gacttcacat tcttggattt
    38641 cactatcaac tctgcgacag tttatatcaa atatgccttt gaatattctg acttgaagaa
    38701 agatacaaca ggcgcttact tcaactatgt gatgaaagaa cttgctcaag gcttcatccg
    38761 aactattgaa cgtgctgttg tcattggtga tggaaaatct aacagtgctg aagacaaaat
    38821 cactgaaatc aaatcaattg ctgaagaaac tgaagcgaat ctctttgaaa cacaagaaat
    38881 caatgttgct ggtgtatttg acaatgctgt tcttgaaacg ctggttgctg gtattgataa
    38941 gatggttcca aatactactc caattcttgt cacttcaaaa gccattgctc gtaaattgaa
    39001 attggtcaaa gatgcagaag gtcgctacat cgatcctcaa ccatttgctc caattgcaac
    39061 caacggaaat gttattgctg gttttcaagt ttacatctat gactggatgg acggtgcaac
    39121 taacccaatc attgcatttg ctgaccaagc ttataagatg attggtgatg aggctgccgc
    39181 tgaccgcttt gatgattatg atgtaacagt caaccgccgt catattgagc ttgctagtgt
    39241 tatgggtggt cgtttggctc agtacaaatc agcggttaag tttacaaacc aagcaggtta
    39301 aatatagaaa ggggagtctg aatgacaatc cttaaaacaa tcaaagaaat ggtagaagtt
    39361 gacgttgaag aggatatctt tgacgtccaa cttttacgat atatcaacag tgggatttca
    39421 tatttacaaa gaaatgctat tcctgttatc cgtatcgata aagaaacgga gttagaggga
    39481 tggacagaca ttaaggaaga agatagggag acagtgcttg actggttaca tctgagatgc
    39541 gtccaacgtt ttgacaaatc tttgatgaca ggcagtgctg ctactatgga atggattgac
    39601 ggagagatga caaatctttt gtatcaactc aaggcaatct atgaagtgaa gacatgaaat
    39661 cttcaagagt agctatcatt ctttgctatg atgagcgtgt tgaggttgaa aaaggtgtct
    39721 gggagaaaaa gaccgtagaa aagaaagtca aagctaagaa agagaaaatc tatcagcgaa
    39781 ggcttgataa agctatggca gacggtcaag ttctgactgc taggtttagt gttcgttcca
    39841 actatgtgac tgacactctt gactatgtca aatataatgg caaagagtac aaggttaaca
    39901 ttgggacaga atcagatgat ggtcattaca ctatcatcga acttggagaa ctgaaataat
    39961 ggccaagaaa tttttcacta gacaagatat tcaatctatc cttgaaacaa accctttgaa
    40021 ggctaaggtc ttctacatgg aacgtgagga gaaatcttct cctgacaatg tcatcttgta
    40081 ttatcggtta acaccaggca gtagcattac tgctgatgat agagtgcaca tgagaaaagt
    40141 gactttgcaa gttagtcact accacaagaa gaaactagac agcattgaaa atttgatgct
    40201 gtctaatttt atgtgtgagc caaatcaatt gaatctaaaa caacctgaca ctgactatct
    40261 actgacaact tacagacttg aggtgtttac aaatgggcgg tggtagcgtg aagatgacac
    40321 cgctaaaggt ggatatcaag aatcaagttt tagagaacat taagaaggct gcccaaagta
    40381 cagaaaatga cataagaacc aaaagcccta gacgaaacgg tgtttacgaa aaaggttgga
    40441 cacacgacat catagatgac gccgctgttg ttcataataa tggcaaagaa aaatctctat
    40501 cccacctatt agagaatggt catgccacta aaaacggtgg ctttgttgcc cctagagagc
    40561 atatcagacc agcctacatc aagaataagg agaaattcct taatgatatg aaatccatta
    40621 aaatcacacc taaataagaa aggagtctta catgacttat caatacgata caagggaagt
    40681 cactcacggt aatgcgaatg gtttttatgc caaaattgct aaaactgatg ctggtgcctt
    40741 agacttgcag aaaccttatc cctttacagg gctacgaagc acatcctttg aaacttctca
    40801 ggaatcaaat gcctactatg cagacaatgt ggaacatgtt cgtttgcaag gtaaaaaatc
    40861 cacagaaggg tctattacaa cctatcagat tcctaaacag tttatgattg atcacttagg
    40921 taaaaagctt acgaactcta caccaccagc tttgattgat actggtgtca atactaactt
    40981 catttggggt tatgctgaaa ctgttacaga tgagtttgga gctgagattg aagagttcca
    41041 catttggacg aacgttaagg catctgcacc taaaggcagc acatcaactg atgaaacatc
    41101 agcgacacca aaagaaattg aaatcccttg tactgcctct ccaaataact tcattgtgga
    41161 ttctgaaaag aaacctgtat cagaaattgt atggcgtgat gacagcaaag gaaccgtccg
    41221 tgggaagttt gacaagttgt ttgctgacaa gtcaccaggc aagctgattg atttcatcaa
    41281 tgaagcgctt ggaacaacca ctacacctac cacaactgtt agcggaggct cttaatgatt
    41341 aaaaaagaac tatcattcac agcgtttgat agttacggtg aagaaagaga gcacactgaa
    41401 acagtgcgct ttctttactc tttaccagct atcaagatgt atgaacagcg aactggtcgt
    41461 aacttctttg atgataacca aaaagcactt acagcatata cacaacttgc tcttgcaact
    41521 ggtgtaaacg gtaacctatc tgatttaact gatgaagaga aaatcaagat gatgcctcta
    41581 cttatggaac ctgatttcat gaacttcttg actgaagtca ttccttgcct ttatggtgag
    41641 gttgaaaatg gacgtttagt acaaaatgag cttaccgctg aaacagcttc attagctcct
    41701 tggtttggtg atttgattga tattggtttc ttctcagacc tcttctatga atttaaccga
    41761 agcagagcaa aggtgccgca agataaaaaa aagccttttc agaagttgta acttccgaaa
    41821 aggtttataa ggttatcttt gaaaatcgga tggatgtttt ttgggcagaa tcccaacact
    41881 ttaactattt gatgggaaca cttcaccaaa tgagcatcaa tcaggaagag aagaaaacct
    41941 tatccaacgc tgatttactt agcgtgatgt cagattaaaa agaaaggagg aaatctatga
    42001 ctgaaacgtt tgaaggctta tacgtcaaat ttggtgctaa tactgttgaa tttgataggt
    42061 ctgtaaaagg tatcaacact gccttatcta gtttgaaaaa agacttcaat aacatcaaca
    42121 gacaattgaa gatggatcca gacaatgttg acttgttgaa tcgtaagttg gttaacttgc
    42181 aagaacaggc tcgtgttggt gctatgaaaa ttgctgaact caaaaagcaa cagaaggcac
    42241 tgggagaatc tgaagttggg tcagcacagt ggaataagct tcaacttgaa attgctaagg
    42301 ttgaatcaca gatgaagatt gttgataagg caatggagtc aacaaagaaa cacattgaag
    42361 atgtaggaga cccaaagtct attctgaatc ttaacaaaga acttgataat gttgctaaag
    42421 agcttgatat tgtcaatcaa aagcttgagc tagacactga caatgtcgaa ctagcagagc
    42481 aaaaaatgaa actacttggt aaacagtcgg aattggttgg ggataaagtc caagaattaa
    42541 agaaaaaaca agctgccctt ggcgatgaga aaataggtac agaagaatgg cgtcaacttc
    42601 aaaatgaaat cggtcaagct gaagttgaag ttctaaagat tgaccgtgca atggacattc
    42661 ttggtgagtc aagccgttct gcaactggag acatcaaaga ggcaaccagc tatttaagag
    42721 ctgatgtcat gatggatgtt gcagataagg ctggtcagat tggccagaaa atggttgacg
    42781 ctgggaaaat gacagtagat gcttggtctg agatagatga ggctctggac accgtcacaa
    42841 ccaaaactgg tctgactggt gatgccttag cagagcttca ggaaattgct aaagacattg
    42901 ctactggtat gcctaccagc tttcagaatg ctggtgatgc cgttggggaa ttgaatactc
    42961 agttcggttt gactggggaa aagctgaaat cagcatctga attacttatc aagtatgctg
    43021 agattaacga aacagacatt tcaagctctg ccatttctgc aaaacaagct attgaagctt
    43081 acggtttgac agctgaagac ttgggaatgg tcttagacaa tgtgaccaaa gccgctcaag
    43141 atacaggaca gtcagttgac acgattgttc aaaaagccat tgacggtgct cctcagatta
    43201 aaggtttggg actttctttt gaagaaggtg ctgcactgat cggtaagttt gagaaaagcg
    43261 gtgtggattc atctgctgct ctatcctctc tatcgaaagc tgctgtcatc tatgctaaag
    43321 acggtaagac tctgacagat ggattgaatg agactgttag tgctattcaa aattctacta
    43381 gtgagacaga ggctttaagt attgcctcag aaatctttgg tagtaaggct gctcctagaa
    43441 tggtcgatgc tattcagcgt ggtgctttta gcttcgatga cttagctgaa gcagctaaaa
    43501 gttcctctgg tactgtctcc accacatttg atgagacgct tgacccaata gataagttga
    43561 ctcagtattc taaccaagca aaagagggaa tggcagaact tggcggtaaa ttgcttgaga
    43621 ctgtcatccc agctttagaa cctttgatgg gtatgcttga atcttctgtc aattggttta
    43681 ctagcctaaa cgaaactgat caacagacta tcgtgattct tggcctagtt acaactgctg
    43741 tgatgatgtt gcttggtgca attgcaccgc tggtcatcgc cataggggca ataggtgcgc
    43801 ctgtcggaat tgtagtggcg gcaatagtag gggctattgc cgtcataaca cttatcatcc
    43861 aagcaatcat gaactgggga gccataactg aatggcttca gtcaacgtgg gattcttgtg
    43921 ctgcctggct ttctgaattg tggactaaca tagtcacgac tgccaccaca gcgtggtcaa
    43981 atttcactgc ctggctttct ggcctttggt cttcagtagt ctcaactgga cagtctttgt
    44041 ggtctagctt tactagttcc ttgtccaata ttttctcaag tttgattaca ggtgctcagt
    44101 ctctgtggtc aagtttcact tccactcttt ccaatttgtg gtctggactg gtctcaactg
    44161 ggtcaaattt gtttaataat ttgagtagca cgatttcagg aatttttaat gggatacttt
    44221 caacagcaag caatatttgg aattccataa aatccactat ttccaatgca atagatgggg
    44281 cgaaaaatgc agtgtccaac ggggtcaatg ccatcaagaa tctgtttaac ttccagatta
    44341 aatggcctca tattccacta cctcacttcc gtgtgagtgg ttctgctaac cctctggatt
    44401 ggctaaaagg tggcttacca agtatcggca ttgactggta tgccaagggc ggtatcatga
    44461 ccaaaccaac cctatttggc atgaatggaa accgtgcaat ggttggcggt gaggctggcg
    44521 ctgaagccat cttgccattg aataagtcaa ccctgggggc aattggtcaa agtattgcta
    44581 acacgatgaa tacatcgaac aatattaacg ttaacttctc tggcgtcact atcagggaag
    44641 aagctgacct taacagacta gccaacgtgg ttggaaatcg tattgctgaa gaattgcaac
    44701 gtaaaactaa tttgagagga ggaatggcat gacaaaaatc aatgaactta ccattgacgg
    44761 tgtgaaaaca tcatctttta agtgtgatgt attggttgaa actagaccaa atgtcattgt
    44821 ctccagctcc aaaacagctc tattagagca tgatggtatt agtggtgcag ttgtgcaatc
    44881 gaatagacac cgtgggctaa ttgagaagcc ttatcatatc actttaattg agccaagcga
    44941 tgaagaaatt tatcgctttt ctgctctttt gaaccgtgaa aagttttggt tggaaaatga
    45001 acaggaacca actattaggc tttggtgtta taaggttgat agctttgaga ttggaaaaga
    45061 cgaatttggt gcctgggtgg tcgatgttac cttcatctgc caccctacca agttttttaa
    45121 aacaactgac acccagactt tgactgggaa tggggttttg agggtgcaag ggtcagctct
    45181 tgctttcccg aagattacag tggttggtca aagcgcttct gagacatcgt ttacggtggg
    45241 gaatcaagtg attaaacttg aaaagctctc agaatcgctt gtgatgacca acgatcctga
    45301 caatcctagc tttaagacgg ctagtggcaa gcttattaag tgggctggtg attttatcac
    45361 agttgatact gctaaggggc agaatgtcgg tgttgtttta ggtgctggca taacgtcctt
    45421 gaaatttgaa acagtttggg ggtgggcata gttgctcttt ttacttgata aagatgttag
    45481 aacggtcaaa tggaatggca ttccacttca cgaggccagc tctgccattg tcaaagaaga
    45541 aaccaacagt gatttctacc tgactgttcg ctatcctatc acggattcag gtatctatca
    45601 acttatcaaa gaggatatgc tgattaaggc gcctacgcct gtgctgggtg ctcagctgtt
    45661 tagaatcaag aaacctattg agaatgatga tagcttggat atcactgctt atcatgtttc
    45721 tgacgacatc atgaagcggt ctatcactcc tgtaagtgta gttggtcaag gttgtgctat
    45781 ggcactgtct cagatggttc aaaatgctaa gactggtcta ggtgattttt cgttcaccag
    45841 cgatattatg gacagcagaa ccttcaacac gacagagacg aaaacactgt attcagtcct
    45901 tatggacggt aaacacagca ttgttggaac gtgggagggt gagcttgtcc gtgacaattt
    45961 tgctctttct atcaagcgta gccgtggggc tgatcgtggt gttgtcatca caacacacaa
    46021 gaacctcaaa tcctatcaac gaaccaagaa ctctcagggt gttgtcacaa ggattcatgc
    46081 acggtcaacc ttcaaacctg atggagctga agatgaagtg acgctcagag tgtctgttga
    46141 cagtccacta ataaactctt atccctacat caatgagaaa gagtatgaga acaacaacgc
    46201 tgaaaccgtt gaagacctta gaaagtgggc tgaggctaag tttacaaatg agggcattga
    46261 taaggtttct gatgccattg agattgaagc ctatgagctt gacggtcaag ttgtaaatct
    46321 gggcgacacg gttaacctca agagtagaaa gcacagtgct gacctctaca agaaggccat
    46381 tgcctacgag tttaacgctt taacggaaga gtatatctcc ataacttttg atgataagcc
    46441 aggagttggt ggttctgggg tgtcaagtgg cttgtctaat gttgctgatg ctatacttgt
    46501 agcaagtgcc acagctcagg acgttgctgt tcaaagggct gtgaaaaatg ctaacgctgc
    46561 ctttgatgca gagtttggta aaacaaaaac aaaaattaat gatgatatcg aaatcgcaaa
    46621 agccaaagta gaaagcttta aatcggaact atctaatcgc atggataatc agcttcttcc
    46681 ccttgcgaca gaagctaaga atttagccag tcaggcacaa gcagacctga ccagaaaaga
    46741 aatagagctt cgagctgaat taaatagaca agtcacaagc acagaaactg taaaaatctc
    46801 tctaacaaat cttagtcaca atatggatat cattaagcaa aaagccctaa acgaccttag
    46861 agacgcagag acaaggctaa aggaagctga tagcgtccaa caacttgcga caaagcgggt
    46921 tgaggataaa ctgacaggac ttagcacgaa actggagtca ttctctgttg gtggctataa
    46981 ttatgtcatt gacggcggag aacctaaaga actaatggct aacttctatg gtaagaccta
    47041 tgatattaat ccccaactac tcgaacgaac tagtcaagcg accttgagtt ttagctatga
    47101 agcagaatca actagtcgtt tagaagttag actttataaa aagatgcaca ccggggacac
    47161 tagtaagatt acaattattg tcatgcctaa ctttgactta tctccaggga aaggatttat
    47221 ctctcagagc tttgatttag gtggtgttat gcctgaccct agaaaccaag cctggcttgt
    47281 gatgcggggg acaaatgcta accccttgac gctctcgaaa gttaagctgg aacgtggtac
    47341 cgttgctaca gactggaata atcgcgatga aaccttaaaa gctagcttcg ccgaatacaa
    47401 gcagaccgtt gatgaaaact tagccaacct tagaacaagt acggaaacct tggcaggtca
    47461 actgacaagt gctgagtcta gcattaggca gacgtcggaa tcctttagca atcgtttggt
    47521 tagtcttgag acctataaag atagtgaatc aaatcgagct agtcgttact ttgaagcaag
    47581 caagtctgaa accgctaagc agctatcagc cttacgaaca gaagttaatg gctcttatgt
    47641 ggataagtca acctatgagg aaaatgcaag aggtttgaca agacgctttg aaagtctgtc
    47701 atcagggaca cagaactatg ctttggggac tgaaagagat gtctatctaa acgtttcagg
    47761 gactaccgtc tatgaccttt atagcttcgg cactacctta gcaataaaag ggattgaaat
    47821 gggagatact gtaactattg catttaacta ttttgcggat agtcggacgg cttttggttc
    47881 ttatgaactg gagctatacg gacatgcagg caaaatcgaa cgggttggtg aggtacagag
    47941 aacgatatca aatcgtggta cctataccgc atcttttgtg gcaaacaatg ccaatttcag
    48001 ggcaaactcc cttaaaatta gatttacgga tagccgactt aagtttagag tgacaacact
    48061 tcgtgtgact aaggggacaa ttcctgcgga ctggagtcca tctccagatg acctaaaagc
    48121 ctactcggat actaagcttg aacagacagc taatgagatt aaagctagtg taacaagtct
    48181 tgatcataaa actcttaagc aaactgatat tactatgact tctgaaggga ttgtcctaag
    48241 agcaggcaag acgagtaatg atgtggcaag agctattggc tcttacttta aggtcactcc
    48301 tgatgctata gctctgttta gctcactgat aaaagttagc gggaacatgt tggttgatgg
    48361 ttctgtaact agtcgtaatt tggtaacagg cgctgtcgaa acgggacatg ttaaggctgg
    48421 agcaatcaca ggagtgcttt tagcagctga agctgtaacg gcagaaaaac ttaaagtcga
    48481 ccaagctttc tttaacaagt tgatggccaa cgatgcctac ttgaagcaac tatttgccaa
    48541 gtcagccttc atcactcagg ttcaatccgt gacaatatct gccagtcaga tttcgggcgg
    48601 tgttattaaa gctttgaata atgccatgga aatccagatg aacagtggtc agatacttta
    48661 ctacactgac caagctgcac tgaaacgggt tttgagtggc tatcctactc agtttgttaa
    48721 gtttgcaact ggtacagtat ctggtaaagg aaatgctggt gttacggtca ttggttctaa
    48781 ccgctacggt acagaatcaa ccaatgatgg tggatttgtt ggagtccgtg catggaacgg
    48841 ctcaaacatt gactcacttg acttagttgg tgataaaatc cgattagcaa gctctgcctt
    48901 tgacaattca gatggttggg atgttcggac acttgattca ggcttgaaaa tcacaccaca
    48961 caatagggct gctgaacgca atagccgaat tgaggttggt gatgtctgga ttttaaaagg
    49021 aaatggatcc tattcttctc tcagagatat tttgaactca ttcaatgaga acttctcaaa
    49081 aggcccaaat gctgactctt acacttacta ccattcgggg ttctaactag aaaggaacaa
    49141 tcatgaacca agaacaacaa ctcaatcaag cgctacgctt gaccgtcaat gaattgacag
    49201 cccagttggc caatgagtca accactaaga acttgctggc tatccagctg actgaagttg
    49261 atcaggagaa acagcaactg actcagcaaa atgctgaact tcaagcaaga gtttccgaac
    49321 tagaaggctt gcttgatgaa caaactcaac cagaaatcat tgaaggagaa taaccatgac
    49381 tacaacagaa aataatttac ttgaccttga aacaatcaca gaaccctttg acttggcgac
    49441 agcactcaag tacatgaagg aaaatggtga gttaatccgt tgcaagaatg ctgtcaatga
    49501 cttctacatg taccgtgaca tgcagaaacg tcctgtcatc gtcaatggtc gccgtcaatt
    49561 caaggacgtg gaaactgtct gggcatttaa ccaatggggc gggacaacac ccacaatcaa
    49621 catggctgat ctctttaatc ttgaatacta catcatgacc tttgatgaga acggaaatcc
    49681 tgactggact gagcctcact tggaggataa gtaagctcat ctaaaataaa caggatagga
    49741 gatagtgaat gccaccatgg ttaatagata gcactgtggt tgtggcgatg gtgacggtgc
    49801 tgggcggtct gttcagcact attatcacca cttcagccaa tcggaaagac cagctgataa
    49861 aacatcagta tgaggatatc aaggaagatt tgtctggctt gattgacaag gttaagacga
    49921 ttgatcatac aaccacggaa acaaagaaaa tttcagaaat cacaaaagac ggtacgctta
    49981 aaatccagcg ttaccgtctt tttcacgatt tgacaaagga aatttctcaa ggctacacaa
    50041 ctattgaaca tttcagggag ttgagcattc tctttgagag ttatcaactg cttggcggaa
    50101 atggggaaat tgaagccttg tttgagaaat tcaaacagtt accaattgag gaggactaaa
    50161 catgacacaa attacagata ttatcatcag ctcagctatg ggaattttga ctattttggc
    50221 aggaattgca gtgcaagctg tcaaggttta tttgattaaa aaaggcggtg aaaaggccgt
    50281 cttgattaca gaaatcttgg ccaagaacgc tgttaatgct gtggagcaag tggccactga
    50341 aactggcttc aaaggagctg acaagctaac ttctgctaag gctcagattt tagctgagtt
    50401 gcaaaaatac aatatccaca tgtcagacga tgatctcact ttgtttgttg aatcatctgt
    50461 caaacaaatg cacgatgcct ggaaggagta acctatggaa atcaacacag aaacagccat
    50521 tgcctggatg tctgcacgtc aaggcaaggt cagctattcc atggactacc gtgacggacc
    50581 taacagctat gactgttcca gctctgtcta ttatgctctc aggtcagctg gtgcaagctc
    50641 agcaggttgg gcggtcaaca ctgagtacat gcacgattgg ctgattaaaa acggttatga
    50701 gcttattgct gaaaacgtgg attggaatgc tgtccgtggt gatatagcaa tttggggcat
    50761 gcgagggcac tcaagcggag ctggtggtca tgtagtcatg tttattgacc ctgaaaatat
    50821 cattcactgt aactgggcaa ataatggcat cacagtgaac aactacaatc agacagcggc
    50881 tgctagtggc tggatgtatt gctacgttta ccgtttgaaa agcggagctt ctacccaagg
    50941 aaaaagcctt gataccttgg tcaaggaaac ccttgctggt aactacggta atggcgaagc
    51001 acgcaaggca gtgcttggca atcaatatga ggctgttatg gcagtcatca atggcaaaac
    51061 tacgactaat caaaagactg ttgaccaact tgctcaagag gtaatcgctg gcaaacatgg
    51121 caacggtgaa gctcgtaaaa agtcgcttgg tagtcaatat gatgcagttc agaaacgagt
    51181 gacggaattg ctcaaaaaac agccctctga gccgtctaag gctcaagagg taaacaagcc
    51241 cacggaaacc aaaacaagcc aaacagagct aactggacaa gccacagcca ccaaagaaga
    51301 gggcgacctc tctttcaatg ggactatctt gaaaaaagcg gtgctggaca agattctggg
    51361 caactgtaaa aagcatgata ttcttccaag ttatgcactg actatcctac actatgaagg
    51421 tctttgggga acttcagcag ttgggaaggc agataataac tggggcggta tgacctggac
    51481 tggtcaaggc aaccgtccaa gcggtgtcac tgtcacacaa ggttccgcac gtccttcaaa
    51541 tgagggcggt cactatatgc actatgcaag cgtggatgac tttctgacag actggtttta
    51601 tcttctaagg gctggtggct cttacaaggt gtctggtgct aaaaccttca gtgaggctat
    51661 caagggaatg tttaaagttg gcggtgctgt ctatgattat gctgcaagcg gctttgatag
    51721 ctatattgtc ggagcttcaa gccgcctcaa ggctattgag gcagaaaatg gttcactgga
    51781 caagtttgat aaaactaccg acattggtga cggtagcaaa gacaagattg acattactat
    51841 tgaaggtatt gaagttacca tcaacggtat cacttatgaa ctgactaaaa agccagttta
    51901 gaattagata cgataagaca aaataagccc tcagcaattg ccgggggctg tttttccgtt
    51961 ataacggact ttcctcaaaa tatcggttat aatggacttt ttttgataaa aattttaaaa
    52021 atatttgtta aaaacaagtg ttttctattg acaagtgttt aaaacaagtg tataatacaa
    52081 ttaaagataa ggaaaggaga taagccaatg acagagcgag agcttaagaa aattgctaag
    52141 aagcaaggtt ttagtaaaac taactttggc aaagggtcac acgaagtttg gaaacatcca
    52201 gacggacgga cagtgacgat acctaagcca aaggaaaaag attacagacc aggcacactg
    52261 aacaacattc tcaaagtctt gtatggggag tgaggacgct cctccccgta cccctaaaag
    52321 gggatactct gatcattggc ttaatctaat attatgaaat acacttattt agcattgttt
    52381 gaagtggata aagaaaacgg tggctataac atttctttcc ctgattttcc tggagcattt
    52441 agtgaagctg atagcttgaa cgaggccatt tttaacgctc gtgaagtcct tgaaatctat
    52501 acaattatgt ttgaagacga gggcaaagag tttcctaaag catcatcatt caaggctctt
    52561 gcaagcaatt tagcaagtga cgaagatgtg attcaggcta tttcagttga tactgagctt
    52621 gtccgtgagc gtgaacgctc taagattgtc aacaagactg tcacactgcc aagctggctt
    52681 gtggaagttg gaaaagaaaa caaggtcaac tttagccagc tgttgcaaaa agcaatccgt
    52741 gaggaattgc aagtataaga aagagcggtc aagatggccg cttttttctg tataaaaaat
    52801 tatgaaaata gatagtaata cttttcaaaa attgacctat gaaaaaccgt ccgccccaaa
    52861 ttcgccccaa attattttta aagttagcct gatttaacct gatggaaaat caaaaaagcc
    52921 tataaaatca ggcttttcat tcggttaatt cctgttaatt caggtactga aaggcggtag
    52981 acggatttga accgacgatc aagcttttgc agagccgtgc cttaccactt ggctataccg
    53041 ccataacaga taatattcta tcgaaaaaaa aaagattcgt caagtctctt tcgtaaaata
    53101 gccaatttta gcccttaaac ttcctcatat taccaaaaaa agagattgat acaatgggta
    53161 tcaatctctt ttagattatt gtctgattaa ataatcaaat gcgccaatgg cagctgtggc
    53221 accagacccc atggaaataa tgatttgctt gtaggcagca tctgtacagt cacctgcagc
    53281 aaaaatacca gggatatttg ttgagccaaa tttatcaacg acaatttctt gacgttcatt
    53341 gagttcaatg ccgctatcct tgagccaaga agtacttggt acaagaccaa tttgtacaaa
    53401 aacaccttct aaatcaatgt gtttttcttc gtttgtatca cgatcggtat agtttaaacc
    53461 agtgacatga tcttcaccaa cgatatcttt agtagcaaca ttcttaagaa tagttaaatt
    53521 gtcagtttta gctgctcgtt cttgcagtac ttggtctgct ttgagctcag gtaagaattc
    53581 taggactgta acatgtttag tgacaccggc tagatctaaa gcagcttcca tacctgaatt
    53641 accaccacct ataacagcaa cattttttcc ttcgaaaaga gggccatcac aatgtggaca
    53701 gtaagtaaca cctttattgc ggaattcttc ctcgccagga acgttgatat ttcgccactt
    53761 agcacccaga gctaggatag cagttttagc ttgtaaaata gctccgttag ccagtgttac
    53821 ctcaactaat tcttttttct caatgccagt tgctaattga ctcttgataa tatcaatatc
    53881 gtaagatttg gtatgctctt caatttgagc cattaattta gggccttcgg tgtacagtgt
    53941 accaatcatg ttctcaatac caactgtttc gataacttgt ccaccaaaag tttctgctaa
    54001 aatacctgtt tttaaccctt tacgagcggc ataaattgca gcactgttac ctgcaggacc
    54061 accaccaata actagaacat catagacacc tttgtcagca aatgcttcag catcaagagg
    54121 tccatctagt tgttctaaaa gctgttcaat tgtagcacgg ccagaagtaa attcttcttg
    54181 gtccttataa acagtaggaa cagacatgat accttttgat ttgacttcgt cttgatacat
    54241 gcctccttca atcatagtat gagtgatatt tggattcaaa acagccatta tattaaaggc
    54301 ttgaacgaca tctggacaat tatggcaagt taatgaaaca taagtttcta gattgatagt
    54361 tttctcaatc cccttaattc gtttgataat atcttcatca acttttggtg cgcgaccaga
    54421 gacttgcaac aaagctaaaa tgaaagatgt gaattcatgc cccattggca agcctgaaaa
    54481 aataacgcga ctttcttgcc ctttttttgc aatcccaaaa ctaggttgac gttttaaatg
    54541 tgttgattca aggctgatac gatcagacat agcgacaatt tcatctagaa aatcctttac
    54601 tttttgagaa ttatcgttat ctcctaaatc agtttgtaat acaatatcag attctagcaa
    54661 atctaaatat tgcgctaatt gagccttgat ttctttatct aagaccatgg attacctcct
    54721 ggttattaaa ttttacctac aaggtctaaa ctaggagtta aagtttcagc tccttctttc
    54781 catttagctg gacaaacttc acctggatgt tggcggatgt attgagcagc acggacttta
    54841 tcaatgagag tacttgcatc acgtccgatg ccatcagcat taatttccat catttggatg
    54901 acaccgtctg gatcaatgat aaatgtacca cgttgtgcta gaccatcttg tccaaggaca
    54961 tcaaaacctt gtgaaataag gtgtgaaggg tcaccaatca taggatatgt gatagtacca
    55021 acgacatctg agtcatcatg ccatgcttta tgaacaaaat gagtatcagt agagacagaa
    55081 taaacttcta catcaagaga tttaagtgtt tcgtattgct cttgaaggtc accaagttca
    55141 gttgggcaaa caaatgaaaa gtctgctggg tagaaacaaa aaactgccca ttttccttta
    55201 acgtcttcat ttgtaacagt gatgaatttt ccatcgtgat aagcttgagc tgaaaattca
    55261 ataatttctt ttccgactaa tgacataata atgtcctcct ttatttttta atagcctaat
    55321 tataaacgaa atacttttaa atctccaata aaaacgttta catttttcta agaaaagtat
    55381 gatttttcta atagagtgga agattttata gcttgtcaaa tctaactttt tttgataaac
    55441 tactatagtt gcttaaagtg acaaatactc atcttagatc aattgtggtc ttgttgccga
    55501 aaggttaggc tgcaagtcga agtctgagag aggaaaaaac atttttaaag gagaacttac
    55561 tcatggcagt aatttcaatg aaacaacttc ttgaggctgg tgttcacttt ggtcaccaaa
    55621 ctcgtcgctg gaaccctaag atggctaagt acatcttcac agaacgtaat ggtatccacg
    55681 ttatcgacct tcaacaaact gtaaaattag ctgaccaagc ttacgaattt gttcgtgatg
    55741 ctgcagcaaa cgatgcagtt atcttgtttg ttggtactaa gaaacaagca gctgaggctg
    55801 ttgctgaaga agctaaacgt gctggtcaat acttcattaa ccaccgttgg ttaggtggaa
    55861 ctcttactaa ctggggaact atccaaaaac gtattgctcg tttgaaagaa atcaaacgta
    55921 tggaagaaga aggaactttt gaacttcttc ctaagaaaga agttgcattg ttaaacaaac
    55981 aacgtgcacg tcttgaaaaa ttcttgggtg gtatcgaaga tatgccacgc atcccagatg
    56041 taatgtatgt tgttgaccca cataaagagc aaatcgctgt taaagaagct aaaaaacttg
    56101 gtattccagt tgtagctatg gttgacacta acgctgatcc agatgatatc gatgttatca
    56161 tcccagcaaa cgatgacgct attcgcgccg ttaaattaat tacttctaaa ttagctgatg
    56221 cagttattga aggtcgtcaa ggtgaagatg ctgacgttga cttcgctcaa gaagcacaag
    56281 cagattcaat tgaagaaatc gttgaagtag ttgaaggatc aaacaacgac taatatttaa
    56341 tttattgtaa atcctaaaac ggggcagatg caaaccgctc tgtctcgttt ttttaatcat
    56401 tatttgaaat taagaggaga acgaaaatgg cagaaatcac agctaaactt gtaaaagaat
    56461 tgcgtgaaaa atcaggtgca ggcgttatgg acgctaaaaa agcattagta gaaactgatg
    56521 gtgaccttga taaagcgatt gaattacttc gcgaaaaagg tatggctaaa gcagctaaaa
    56581 aagcagaccg tgttgctgct gaaggtttaa caggtgttta tgttgatggt aacgttgcag
    56641 cagttattga agttaacgct gagactgact ttgttgctaa aaacgaccaa tttgtaacat
    56701 tagttaatga aactgctaaa gttattgcag aaggacgtcc ttcaaacaat gaagaagcac
    56761 ttgctttaac aatgccttca ggtgaaactt tagaacaagc atttgtaact gcaactgcta
    56821 caattggtga aaaaatttca ttccgtcgct tcgctttagt tgaaaaaact gatgagcaac
    56881 attttggtgc ataccaacat aatggtggac gtatcggagt aattactgtt gttgaaggtg
    56941 gcgacgatgc acttgctaaa caagtatcaa tgcacgttgc agctatgaag ccaactgttc
    57001 tttcatatac tgaacttgat gcacaatttg ttcatgatga attagcacag ttgaaccaca
    57061 aaattgagca agacaacgag agtcgtgcta tggttaacaa accagctctt ccattcctta
    57121 aatatggatc aaaagctcaa ttgactgatg aagttattgc ccaagcagaa gaagacatca
    57181 aagcagaatt agctgcagaa ggtaaaccag aaaaaatctg ggataaaatc gttccaggta
    57241 aaatggatcg tttcatgctt gacaacacta aagttgacca agagtacaca ctccttgctc
    57301 aagtttacat catggatgat agcaaaacag ttgaagctta tcttgagtca gtaaatgcta
    57361 aagcagttgc tttcgtacgt tttgaagttg gagaaggtat tgagaaagct tctaacgact
    57421 ttgaagcaga agttgcagct acaatggcag cagcacttga aaaataatta acactcgctt
    57481 tatattataa tttggcagca aaatgttgtc agtaaatata ttttaaaata agacacttgg
    57541 cttaagtcaa gtgtcttatt ttgctatcga attggtggat tggcaaatat taaaaaggag
    57601 agacagtcat ctcaccttaa cttttctaga ataggttgaa gccgataatg gataatgttc
    57661 ccatgatgca tccagctagt ataacaccta gcataacagc aataacacta tgtttaaatt
    57721 cccaatctag gaggctagct gcaagtgtgc cagtccatgc tcctgttccg ggtaagggaa
    57781 tgcctacaaa caatagcaag gcaataaaca atcctttttc tccagctact ttttcaagtt
    57841 tttgaccacc gctatgtcct tttttaaggc accatgtgaa aaacttacca gtataaggtt
    57901 tatctgctcc ccattctaga actttgcgag caaagaagaa gatgataggt acaggtagca
    57961 tgtttccaac aacaccgata gctagcgctt cccacaaagg tattccatta gcgattgcaa
    58021 atggcacagc tcctcgtagt tccactaagg gaatcattga aatcaaaaat gtaataatgt
    58081 aattcattaa ataaaacctt tctatggtat tattccattt tatcttatct tgctagtaat
    58141 gacaactaaa atccgcggtc attctagttt tactatcc
//
LOCUS       DATROD010000004        56141 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.004, whole genome shotgun sequence.
ACCESSION   DATROD010000004 DATROD010000000
VERSION     DATROD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 56141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56141
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.004"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(122..412)
                     /locus_tag="VBC74_000224"
     CDS             complement(122..412)
                     /locus_tag="VBC74_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEO6941207.1"
                     /translation="MSQIKLTPEELRISAQKYTTGSQSITDVLTVLTQEQAVIDENWD
                     GTAFDSFEAQFNELSPKITQFAQLLEDINQQLLKVADVVEQTDSDIASQINK"
     gene            complement(867..1259)
                     /gene="rpsI"
                     /locus_tag="VBC74_000225"
     CDS             complement(867..1259)
                     /gene="rpsI"
                     /locus_tag="VBC74_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEO6941208.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITINKKDVEEYIPHADL
                     RLVINQPFAVTSTQGSYDVFVNVVGGGYAGQSGAIRHGISRALLEVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1280..1726)
                     /gene="rplM"
                     /locus_tag="VBC74_000226"
     CDS             complement(1280..1726)
                     /gene="rplM"
                     /locus_tag="VBC74_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEO6941209.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKASDKIYYTHSMYPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            complement(1948..2070)
                     /locus_tag="VBC74_000227"
     CDS             complement(1948..2070)
                     /locus_tag="VBC74_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002278327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6941210.1"
                     /translation="MKNLRLKTARASMDLLQQSLAEKVGVSCQTIAAIEKGGYN"
     gene            complement(2607..3467)
                     /locus_tag="VBC74_000228"
     CDS             complement(2607..3467)
                     /locus_tag="VBC74_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO6941211.1"
                     /translation="MTFKILTDSTSDLDEKWAQEHNVDIIGLTIELDGKTYETVGDEK
                     ITSDFLLERMQEGAKPTTSQINVGQFEEVFSTYAENDHALLYLALSSHLSGTYQSATI
                     AREMVLDKYPDAQIEIVDTMAASCGEGVLAMLATKERQEGKSLEEVKQKIESLLPKLN
                     TYFLVDDLNHLMRSGRLSKGAAIIGSVAKIKPLLKLDSEGKLVPFAKTRGRKKGIKEI
                     VTQATKTLSYSTLIIAYSGEKDSAQVMKEQLLADERIEEVIIRPLGPVISAHVGSGAL
                     ALFSLGEENR"
     gene            complement(3560..4078)
                     /locus_tag="VBC74_000229"
     CDS             complement(3560..4078)
                     /locus_tag="VBC74_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEO6941212.1"
                     /translation="MKKHSILLVDGYNMIAFWKDTRQLFKSNRLEEAREVLLRKLNHY
                     AHFEHIDIICVFDAQYVPGVRQRYDQYKISVIFTEEDETADSYIERAAAELNQSVLNL
                     VSVATSDLNEQWTIFSQGALRVSARELEQRVATVKSDLDKMSSQIDLSTPKLRPWNDE
                     QLGKLKDFLDGM"
     gene            complement(4075..4830)
                     /gene="rlmB"
                     /locus_tag="VBC74_000230"
     CDS             complement(4075..4830)
                     /gene="rlmB"
                     /locus_tag="VBC74_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEO6941213.1"
                     /translation="MTMKDKQFKEESSDLVYGLHAVTESLRANTGNKLYLQDDLRGKN
                     VDKVKALATEKKVSISWTPKKILSDMTNGGVHQGFVLKVSEFAYADLSEIMTKAENEE
                     NPLILILDGLTDPHNLGSILRTADATNVTGIIIPKHRSVGVTPVVSKTSTGAVEHVPI
                     ARVTNLSQTLDTLKDKDFWIFGTDMNGTPSHKWNTKGKLALVIGNEGKGISHNIKKQV
                     DEMITIPMNGHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(4933..5319)
                     /locus_tag="VBC74_000231"
     CDS             complement(4933..5319)
                     /locus_tag="VBC74_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEO6941214.1"
                     /translation="MIDVRLINGIALAFEGDAVYSLYIRRHLIMQGFTKPNQLHRKAT
                     QYVSANAQALLINAMLEENILTDEEQLIYKRGRNANSHTKAKNADIITYRMSTGFEAL
                     MGYLDMTGQIKRLETLIQWCIETIEK"
     gene            complement(5312..6655)
                     /locus_tag="VBC74_000232"
     CDS             complement(5312..6655)
                     /locus_tag="VBC74_000232"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEO6941215.1"
                     /translation="MIKIYDTMTRSLQDFIPLNEGKVNMYVCGPTVYNYIHIGNARSV
                     VAFDTIRRYFEYCGYQVNYISNFTDVDDKIIKGAAEAGMDTKSFSDKFISAFMEDVAA
                     LGVKPATKNPRVIDYMDEIIDFVKVLVDKEFAYEANGDVYFRVSKSHHYAKLANKTLE
                     DLEIGASGRVDGEGEIKENPLDFALWKSAKSGEVSWESPWGKGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVNVDNEKMSKSLGNF
                     ITVHDMLKSVDGQVIRFFLATQQYRKPVNFTEKAVHDAEVNLKYLKNTFNLPIQENAN
                     DEELEQFVKAFQEAMDDDFNTANGITVIFEMAKWINSGHYTSKVKETLAELLEIFGIV
                     FQEEVLDADIESLIEQRQEARANRDFATADRIRDELAKQGIKLLDTKDGVRWTRD"
     gene            complement(6652..6834)
                     /locus_tag="VBC74_000233"
     CDS             complement(6652..6834)
                     /locus_tag="VBC74_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941216.1"
                     /translation="MKDYVTTQILSILGILAGACLSYLKFAQGNSLLGIIWAAFALMY
                     IVRLYKAYQKQKKEKP"
     gene            complement(6844..7428)
                     /gene="cysE"
                     /locus_tag="VBC74_000234"
     CDS             complement(6844..7428)
                     /gene="cysE"
                     /locus_tag="VBC74_000234"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEO6941217.1"
                     /translation="MGWWKESIAIVKEQDPAARSSLEVILTYPGIKALAAHRLSHFLW
                     NHNFKLLARMHSQFWRFWTQIEIHPGATISEGVFIDHGSGLVIGETAIVEKGAMLYHG
                     VTLGGTGKDKGKRHPTIRKGALISAHSQIIGPIEVGENAKVGAAAVVLADVPADVTVV
                     GVPAKVVRVHGQKDDLQIRSIEHDREESYYSSKL"
     gene            complement(7437..8189)
                     /locus_tag="VBC74_000235"
     CDS             complement(7437..8189)
                     /locus_tag="VBC74_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEO6941218.1"
                     /translation="MTSTNELDIRLRAFINAPDNFLDSIGLVNALHHSTVWASKEPYA
                     IQVDGQEVVPVFTDITDLNHFKEEQESARDMFWESRRSLDVLDEAISHGLAGLVYNLK
                     KEGDFGNSTIFYCEDMVQFMNNYTTILNQLLNEDNIVADIMDKTYLVPAFVHPREEGS
                     FDRFFPTMSTPEGKSYVPVFSNLLSFEKWYNHNDFGGAFRKAQGVILAWTIDDIYKPR
                     NGENEIDDTFGVAINPFDEQQVLVDWSDVEND"
     gene            complement(8191..10320)
                     /gene="pnp"
                     /locus_tag="VBC74_000236"
     CDS             complement(8191..10320)
                     /gene="pnp"
                     /locus_tag="VBC74_000236"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEO6941219.1"
                     /translation="MSKQVFEMIFAGKKLVVETGQVAKQANGSVVVRYGDSTVLTAAV
                     MSKKMSTGDFFPLQVNYEEKMYAAGKFPGGFNKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSFDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVAYVDGNFI
                     INPTAQEQEASALELTVAGTKEAINMVESGAKELSEEIMLEALLKGHEAVCELIAFQE
                     EIVTAIGKEKAEVELLQVDPELQAEIIATHNIALQAAVQVEEKKAREAATEAVKEVVI
                     GEYEARYAEHEEYDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRRVDEIRPLDAVI
                     DFLPQVHGSGLFTRGQTQALSVLTLAPMGEAQIIDGLTPEYKKRFMHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIEGITPQILEEALAQAKKARFEILDVLHGAIAESRPQLAPTAPKIDMIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDEEGNVSIFSSDQAAIDRTKDIIASLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTANVADVLEIGEEVDVKVIKI
                     DDKGRVDASMKALLPRPPKADNPKKES"
     gene            complement(10700..10969)
                     /gene="rpsO"
                     /locus_tag="VBC74_000237"
     CDS             complement(10700..10969)
                     /gene="rpsO"
                     /locus_tag="VBC74_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEO6941220.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNDHIK
                     QHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            complement(11057..12316)
                     /locus_tag="VBC74_000238"
     CDS             complement(11057..12316)
                     /locus_tag="VBC74_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEO6941221.1"
                     /translation="MRAWKGIVLILSSIVVTLVAWQNAGLSEFVVPGLALTSLSLTFL
                     LSTKFRILESYFQGIENMYFYHKVMAVFSMILLLLHKIGLVQGGHGSEFAKTIGSAGL
                     YLFLSIVFVAYFGNFLKYEIWRFIHRFVYLVYILGLVHTFLILGDRILGNTLLSLIVL
                     GYAVIGVISGFYIIFLYSRMRFRRVGYVQKVTHLNHDTTEIEIAMKRPYRYDYGQFTF
                     LKIYQAGFESAAHPFSISGGHDRVIFLTVKASGDYTKSIYKQLKVGTKIALDRAYGHM
                     LFDKDKKEQVWIAGGIGITPFISFIRENSILTKRVDFFYTFSNQDNLIYQDMLESYAK
                     ANPNFKLHLNNSSLQGRLDFSQSVFEGQPTIFMCGPTSMSSTYAKVFRQKDAKSRLVY
                     EGFSFRDSWLSIFLLKTFDKVYSNLIK"
     gene            complement(12425..13354)
                     /locus_tag="VBC74_000239"
     CDS             complement(12425..13354)
                     /locus_tag="VBC74_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEO6941222.1"
                     /translation="MRCSTKEMRLVYRDFLLQANQENKQITVLEADLSSSMSTNVLAS
                     EFGKRYINLGIMEAEMVGLAAGLAIKGYKPYLHTFGPFASRRVFDQVFLSLGYSQLSA
                     TIIGSDAGVSAEMNGGTHMPFEELGLLRLIPKATIFEVSDDIQFEAILKQTLSIDGLK
                     YIRTIRKAPTAVYEGCEDFSKGFIQLRQGKDIALVASGIMVSRAIEAADYLKELGIEA
                     SVIDLFKIKPLPEELKPLLIDQSIVTIENHNRIGGIGSALCEWLSMEKDTTVSRMGID
                     ERFGQVGQMEYLLEEYGLAVKDIVEHCKSIYKS"
     gene            complement(13351..14208)
                     /locus_tag="VBC74_000240"
     CDS             complement(13351..14208)
                     /locus_tag="VBC74_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEO6941223.1"
                     /translation="MTSPEERYSSLRRFATEIRLNTLETLNHLGFGHYGGSLSIVETL
                     AILYGDIMDINPEKFKESDRNYMVLSKGHAGPALYSTLYLKGFFDKTFLHSLNTNGTK
                     LPSHPDRNLTPGIDVTTGSLGQGISIATGIAYAQKIENSSYYTYTIVGDGELNEGQCW
                     EAIQFAAHHQLHHLIVFVDDNKKQLDGLTADICNPGDFVAKFEAFGFDAVRVKGDDIE
                     AIDKAIKTFQDSNSVKPKCIVLDSIKGQGVKELEELASNHHLRPDLQQKTMLERAVIS
                     LRESLEVVE"
     gene            complement(14211..15566)
                     /locus_tag="VBC74_000241"
     CDS             complement(14211..15566)
                     /locus_tag="VBC74_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEO6941224.1"
                     /translation="MKGLLDFLVNIARTPAILVALIAIIGLVLQKKGVPDIVKGGIKT
                     FVGFLVVSGGAGIVQNSLNPFGKMFEHAFHLVGVVPNNEAIVAVALTKYGSATALIML
                     AGMIFNILIARFTKFKYIFLTGHHTLYMACMIAVIFAVAGFTSFSLILFGSLALGIIM
                     SVSPAFVQKYMIQLTGNDKVALGHFGSLGYWLSGFIGGIVGDKSKSTEDIKFPKSLSF
                     LRDSTVSITISMAIIYLIVAVFAGEAYIAKEISNGVNGLVYALQLAGQFAAGVFVILA
                     GVRLILGEIVPAFKGISEKLVPNSKPALDCPIVYPYAPNAVLIGFISSFVGGLVSMIV
                     MIVTGTTVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFVQGILISFLPIFLMPVL
                     GGLGFKGSTFSDADFGLTGIILGALNHVGGAIAIVIGIVVILIGLFGISFVGKSTHKE
                     G"
     gene            complement(15579..15863)
                     /locus_tag="VBC74_000242"
     CDS             complement(15579..15863)
                     /locus_tag="VBC74_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6941225.1"
                     /translation="MLRIGTACGSGLGSSFMVQMNIESILKDLGVSDVEVEHYDLGGA
                     DPSAADVWIVGRDLEDSAGHLGDVRILNSIIDMDELRELVTGICQEKGLI"
     gene            complement(15866..17902)
                     /locus_tag="VBC74_000243"
     CDS             complement(15866..17902)
                     /locus_tag="VBC74_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEO6941226.1"
                     /translation="MVDNKTVVIMLVFLARKNLSLYELTVQTKFSIKVIIEQINYLNS
                     FLAKNHLPAIAHSAGRYQLLGDEKEHDKIVSLLEAEQFYLTQEERVCLIYLYSFCRRE
                     FVSNVHYQDFLKVSKNTTLSDIKMLRSKLAKRGISLTYTRAKGYSLVGDEMDKHQVAF
                     QMITQLLESPIGFWSLNYILSSWKFALSYEKLEKTVEYFYESFQLSPIQDRLEKSLYF
                     IILILCRYQRSVDRVLQGSPIVSEQLKELTTIIVTNLSQDISLSKPLDQKEKDYITLI
                     LSGCFEGEGTKDDDFFEALAKAIVDEMETVSLLNFSNKEELLQGLKRHIIPAYFRLKY
                     GLTGDSGYTQNIKEHYSDLFLLVKKALRPLEEQVGLIPDSEISYFVIHFGGYLRQSGD
                     TQSMSYKALILCPNGVSSSLVIKEKLRGLFPQIHFHRVSKIEQLKLIDNQTYDMVFST
                     IFVETKKPNYLVSLMMTAEQVQQLKELVISDFPKACLDDFQLDQLIATIKKYAHVHCE
                     EELKLALRTMVKQDIFRKDVRPLLHQLITEETYQTSSEQMNWKEAIRLAAKPLLASGK
                     ITESYPEAMIEKVEEFGPFINLGKGIAIPHARPEDGVNSVGMSMLVLEQPIYLLDDPK
                     QEIYLLLCIAAIDNETHLKALSHLTTILRDNNNVKALLASRGYQDIEMIIKQED"
     gene            complement(18118..19743)
                     /gene="treC"
                     /locus_tag="VBC74_000244"
     CDS             complement(18118..19743)
                     /gene="treC"
                     /locus_tag="VBC74_000244"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEO6941227.1"
                     /translation="MTIDKRKVVYQIYPKSYKDTTGNGVGDLRGIIEKLPYLAELGID
                     MVWLNPFYPSPQRDNGYDISDYTAINPDFGTMDDFEEMIEVGRQYRIDFMLDMVLNHC
                     SIEHEWFKKALAGDRYYQDFFILRDNPTDWVSKFGGNAWSPFGDTGKYYLHLFDITQA
                     DLNWRNADVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEILENCPINDGKPAYTDRP
                     ITHDYLKMLNNASFGQADSFMTVGEMSSTTIANCILYTAPEREELSMAFNFHHLKVDY
                     KDGQKWTIMAFDFPALRDLFHSWGEGMSEGNGWNALFYNNHDQPRALNRFVDVKRFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDYSSMDDYVDIESLNAYQIMLDEGKSQE
                     EAFSIIRAKSRDNSRVPMQWDDSTNAGFSEGAPWLKVGKSYKEINVAKEKTGLIFTFY
                     QELIRLRKQLPIIADGNYKAAFKDNEKVYAFERHLDKEKLLVLNNFFAEKVKIKLPEN
                     YLQGQVLLSNYKDVTLDETVTLQPYQTLAILVS"
     gene            complement(19965..21995)
                     /gene="treP"
                     /locus_tag="VBC74_000245"
     CDS             complement(19965..21995)
                     /gene="treP"
                     /locus_tag="VBC74_000245"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEO6941228.1"
                     /translation="MEQFKHDAKALLEAIGGKENISAVTHCATRMRFVLNDSSKAKVK
                     VIEELPSVKGTFTNAGQFQVIIGNDVPIFYNAFVAVSGIEGVSKEAAKSAAPKNQNPL
                     QRVLTMLAEIFTPIIPAIIVGGLILGFRNILDAVPFEFLGQKVVDGVRQVDSSGHPIW
                     NTLVDVSTFWSGVDSFLWLPGEAIFHFLPVGIVWSVTRKMGTTQILGIVLGICLVSPQ
                     LLNAYSVASTSAADIAKNWSWNFGYFTVQKIGYQAQVIPALLAGLSLSYLEIFWRKHI
                     PEVVSMIFVPFLSLVPAIILAHTVLGPIGWTLGKWISAIVLIGLTGPVKWLFGAIFGA
                     LYAPFVITGLHHMTNAIDTQLIADTKTHTTGLWPMIALSNIAQGSAVLAYYFMHRHDE
                     KEAQISLPAAISAYLGVTEPALFGVNVKYIYPFVAGMIGSSVAGLLATTFNVQANSIG
                     VGGLPGFLSINVKYMGYFFICMAVAIFIPLFLTLFFKKSGILTKTEEEKLVSDAVIAS
                     TTETKSAKEKAVVSGTKLSVVSPLSGLAKPLDQASDPVFSQGIMGKGVVIDPSDGELV
                     SPVDATVSVLFPTKHAIGLLTSEGVEFLIHIGMDTVNLEGKGFTSHVAQGDNVKVGDK
                     LITFDIPMIKEEGYIVETPILITNQEEFRPEELIDLPKQIKRGQALMVAKKI"
     gene            complement(22277..22903)
                     /locus_tag="VBC74_000246"
     CDS             complement(22277..22903)
                     /locus_tag="VBC74_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6941229.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEINLEVAPGQVLGIFGQSGCGKTS
                     LSKVLAGFLHPRSGEVLVDGSNLPNKAFRPVQLIQQHPEKTMNPLWPMKKSLEEAYYP
                     SRDLLDAFGIQEKWLNRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIVISHDFAMLEKLCNQCYMIEENRIVSFNGD"
     gene            complement(22887..23690)
                     /locus_tag="VBC74_000247"
     CDS             complement(22887..23690)
                     /locus_tag="VBC74_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6941230.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEIKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNASVTGDMIYRGQSLNSKRIKQLRGKDITLIPQSVN
                     YLDPSMKVKHQVRLGISENSKATQEGLFQQFGLKESDGDLYPFQLSGGMLRRVLFTTC
                     ISDKVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGNGEQLQTEFARSLWRSLPQQEFLKGVTHDLRG"
     gene            complement(23702..24523)
                     /locus_tag="VBC74_000248"
     CDS             complement(23702..24523)
                     /locus_tag="VBC74_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6941231.1"
                     /translation="MILRRRTIVLWQLGIAISLILSILALNLYFHSTPLQTNAALRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVILATVFGVLAGLGNSIID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYHLKN
                     KEFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVIFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(24520..25497)
                     /locus_tag="VBC74_000249"
     CDS             complement(24520..25497)
                     /locus_tag="VBC74_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6941232.1"
                     /translation="MKHITTVVIWKILRCLSLVFGVSILTFILLKQSPVDPVMASVNY
                     DTSLTPVQYKAIAHHYGLDKPAPVQYFIWLKNFIQGHLGTSLVYRQPVIDIIRSRAGA
                     SFILMGLSWVLSGLIGFILGTLSAFHQGKLLDRIVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRIKHLILPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETEWQIFKNHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            complement(25610..27238)
                     /locus_tag="VBC74_000250"
     CDS             complement(25610..27238)
                     /locus_tag="VBC74_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6941233.1"
                     /translation="MTKYLKYISFVALFLASIFLVACQNQNSQTKERTRKQRPKDELV
                     VSMGAKLPHEFDPKDRYGIHNEGNITHSTLLKRSPELDIKGELAKKYKISKDGLTWSF
                     DLNDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQVNIHLTEAHS
                     TFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELASKKVKGTRLLDIASNDVRGLSLPYVKK
                     GVVKNSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDRTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGNLKAEFDLYYPTNDQLRANLAVEVA
                     EQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHYPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSPDLDKANKYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINVGKQGVHSHGHDWSLLTNIAEWTWDESAK"
     gene            complement(27481..28209)
                     /gene="lrgB"
                     /locus_tag="VBC74_000251"
     CDS             complement(27481..28209)
                     /gene="lrgB"
                     /locus_tag="VBC74_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HEO6941234.1"
                     /translation="MELLKTPIFGICFSLILYTIGQHLFKKSKGFFLLQPLFFAMVSG
                     IVILWLMSKGLGTDVKTFYTQAYKPGGDLIFWFLNPATIAFAVPLYKKNDVVKKYWVE
                     ILSSLVIGMIVSLMLIVAISKMVGLSQVGIASMLPQAATTAIALPITAAIGGNTAVTA
                     MACILNAVIIYALGKKLVSFFHLNDSKIGAGLGLGTSGHTVGAAFALELGELQGAMAA
                     IAVVVIGLVVDLVIPIFSHLIGLL"
     gene            complement(28211..28666)
                     /locus_tag="VBC74_000252"
     CDS             complement(28211..28666)
                     /locus_tag="VBC74_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO6941235.1"
                     /translation="MKHTSKETKTKEAPMFIQMSIYAAILLVSQMISMLLPKSLPIPT
                     TVIGLVLMYVLLTAKIIKVEWVDSFGALMISMIGFMFVPSGISVAANLDILKAEGLQL
                     VAVITISTVVMLVVVAYVARLILAIRELSVTDLFKKILPNHYSKKVEEK"
     gene            complement(28836..29576)
                     /locus_tag="VBC74_000253"
     CDS             complement(28836..29576)
                     /locus_tag="VBC74_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEO6941236.1"
                     /translation="MKILILDDEMFARQELSFLVEHSQEVDNPEIFQAEDISEAEKIL
                     FRQQIDLIFLDISLSEENGFTLANQLSQLAHPPLVVFATAYDNYAVKAFESNAVDYIM
                     KPFEQQRVDMALSKVKKLSQLTTASDVEQAIPKKASAELLTLTLSDRSVVVKMQDIVA
                     ASVEDGELTVSTVQKTYTIRKTLNWFKSRAVAPYFLQIHRNTVINLEMIEEIQPWFNH
                     TLLLIMSNGEKFPVGRSYLKDLNEHLTM"
     gene            complement(29557..31302)
                     /locus_tag="VBC74_000254"
     CDS             complement(29557..31302)
                     /locus_tag="VBC74_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEO6941237.1"
                     /translation="MLMVLLFQRLGIIMILAFLLVNNSYFRQLIEERSKRETVVLVII
                     FGLFVIISNITGIEIKGDRSLVERPFLTTISHSDSLANTRTLVITTASLVGGPLVGSI
                     VGFIGGVHRFFQGSFSGSFYIVSSVLVGIVSGKIGDKLKENHLYPSTSQVILISIIAE
                     SIQMLFVGIFTGWELVKMIVIPMMILNSLGSTLFLAILKTYLSNESQLRAVQTRDVLE
                     LTRQTLPYLRQGLTPQSARSVCEIIKRHTNFDAVGLTDRSNVLAHIGVGHDHHIAGQP
                     VKTDLSKSVIFDGEPRIAQDKAAISCPDHNCQLNSAIVVPLKINDKTVCALKMYFAGD
                     KTMSEVEENLVLGLAQIFSGQLAMGITEEQNKLASMAEIKALQAQINPHFFFNAINTI
                     SALIRIDSDKARYALMQLSTFFRTSLQGGQDREVTLEQEKSHVDAYMNVEKLRFPDKY
                     QLSYDISAPEKMKLPPFGLQVLVENAVRHAFKERKTDNHILVQIKPDGHYYCVSVSDN
                     GQGISDTIIDKLGQETVAESKGTGTALVNLNNRLNLLYGSVSCLHFSSDKNGTKVWYR
                     IPNRIREDEHENFNS"
     gene            complement(31329..31973)
                     /locus_tag="VBC74_000255"
     CDS             complement(31329..31973)
                     /locus_tag="VBC74_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6941238.1"
                     /translation="MEKVIIFDMDGVIVDSEYTFLDNKTEMLREEGIDTDVSYQYQYM
                     GTTFEFMWQAMKEEFGLPKTVKEYIAEMNRRRQAIVTRDGVRPIKGAQQLIHWLHQHG
                     YRLAVASSSPMVDIKRNLKELGVTECFEYMVTGEDVSSSKPAPDVFLRAAELLDVDPK
                     VCIVIEDTRNGSLAAKAAGMYCFGFANPDYPPQDLSMADKVISDYQDIYIYLPE"
     gene            complement(32097..32492)
                     /locus_tag="VBC74_000256"
     CDS             complement(32097..32492)
                     /locus_tag="VBC74_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO6941239.1"
                     /translation="MYNKVIMIGRLTAKPEMVKTPTDKSVTRATVAVNRRFKGSNGER
                     EADFINVVMWGRLAETLASYGTKGSLISIDGELRTRKYEKDGQTHYITEVLASSFQLL
                     ESRAQRAMRENNVSGDLSDLVLEEEELPF"
     gene            32573..33289
                     /locus_tag="VBC74_000257"
     CDS             32573..33289
                     /locus_tag="VBC74_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO6941240.1"
                     /translation="MTNVTSYKEMLAKPWGKIQYEITFAQLSHIKNQNVLDFGAGFCL
                     TAQHLAKENNVTAIEPNPKLLYDNQSDNIYKILGSYEALRALPDQSFDTIICHNVLEY
                     IDKHNHPAYFDEFSRLLKPNGELSLIKHNIIGKILQSVIFSNDTSTAMELLTGEANFK
                     SASFDQGNIYTLEELKQNTNLLVERYQGIRTFYSLQPNHFKTETDWLNKMLAIELSVA
                     DKAPYKDIAFLQHITLKKSL"
     gene            complement(33343..33969)
                     /locus_tag="VBC74_000258"
     CDS             complement(33343..33969)
                     /locus_tag="VBC74_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEO6941241.1"
                     /translation="MIFTYNREHVGDTLMVIVKDSQGAKLDVDRRGQVARVYLQDSKE
                     TVAWNIFEVSSLIAIEGVGQVTLSDQVIKILNAELLKEGFEDSLVNNIEPTFVVAQIK
                     EMIDHPDSDHLHICQVEINDGKTVQIVCGAPNASVGLKTVAALPGAMMPNGSLIFPGK
                     LRGEDSFGMLCSARELALPNAPQVRGIIELSDQVIVGESFDANKHWKN"
     gene            complement(34002..34325)
                     /locus_tag="VBC74_000259"
     CDS             complement(34002..34325)
                     /locus_tag="VBC74_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEO6941242.1"
                     /translation="MILPESYEEIAAYIDSTKKVVFFFTADWCPDCQFIYPVMPSIEK
                     DFSDFVFVRVNRDDYIELAQQWNIFGIPSFVVVENGQELGRLVNKNRKTKAEITKFLA
                     EINHK"
     gene            complement(34322..34606)
                     /locus_tag="VBC74_000260"
     CDS             complement(34322..34606)
                     /locus_tag="VBC74_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEO6941243.1"
                     /translation="MKNKKILFGTGLAGVGLLAAAGYTLTKKVTDYKRQQITQTLREL
                     FSQMGDIQVFYFNEFESDIKMTSGGLVLEDGRIFEFIYRQGVLDYVEVSK"
     gene            34767..35006
                     /locus_tag="VBC74_000261"
     CDS             34767..35006
                     /locus_tag="VBC74_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941244.1"
                     /translation="MKEKLLVPALNCLLGLLFLGDHWFLFRLQNGELFAIVTLILLCT
                     ASFLINKNHPKNLTLIAMVITFLAVLSFLSTFLLA"
     gene            35191..36258
                     /gene="pepA"
                     /locus_tag="VBC74_000262"
     CDS             35191..36258
                     /gene="pepA"
                     /locus_tag="VBC74_000262"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEO6941245.1"
                     /translation="MSDLFNKIKTVTELDGIAGYEHNIRNFLRQEITPLVDQVETDGL
                     GGIFGVKNTHETNAPKVMVAAHMDEVGFMVSHIQPDGTFRVLEVGGWNPLVVSSQRFT
                     LYTRSGDAIPVISGSVPPHFLRGQSGGTTLPKISDIVFDGGFTDKNEAESFGIAPGDI
                     IVPKSETILTANQKHIMSKAWDNRYGVLMVTELLKSLKDQSLSNTLIAGANVQEEVGL
                     RGAHVSTTKFNPDIFLAVDCSPAGDIYGEQGKIGEGTLIRFYDPGHIMLKDMRDFLLT
                     TAEEAGIKYQYYAAKGGTDAGAAHLKNSGIPSTTIGVCARYIHSHQTLYAMDDFLQAQ
                     AYLQAIVNKLDRSTVDIIKGY"
     gene            36328..37098
                     /locus_tag="VBC74_000263"
     CDS             36328..37098
                     /locus_tag="VBC74_000263"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEO6941246.1"
                     /translation="MKIGIIGVGKMASAIIQGLKQTQHDIVISGSCLERSKEIAERLD
                     VTYAESHQSLINQADIIMLGIKPQLFEKVLLPLDITKPIISMAAGISLARLSQLTRSD
                     LPLIRIMPNINAQILQSCTAICYNNHVSDELRQLAKEITDSFGSSFDIAEINFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKYGFPKEQALSIVGQTVLASSQNLLQGQNSTSDLIDN
                     ICSPGGTTIAGLLDLEKNGLTHSVISAIDATIEKAKKL"
     gene            complement(37119..37784)
                     /locus_tag="VBC74_000264"
     CDS             complement(37119..37784)
                     /locus_tag="VBC74_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6941247.1"
                     /translation="MSFIIKKGKILALLIAFLVINQLVPILAVWLLKNHYQTPFTSIL
                     LIGLELLIIALFLYYAKVKQIIRWKVLLTRKALVTILLGWLSLRVPQIIGYLIMTMQG
                     VKDTANQTVIMELTKQLPLALMLIFAIIGAPIMEEIIFRYIIPKELFAKHQKWGFVIG
                     TLAFALIHSPSDIGSFIIYAGMGAILSFVYYKTEHLEYSIMIHFINNALAYSVLISTM
                     MNN"
     gene            complement(37853..38308)
                     /locus_tag="VBC74_000265"
     CDS             complement(37853..38308)
                     /locus_tag="VBC74_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941248.1"
                     /translation="MLIKGLLSPNSKVSPKDYKVYMRFLSFVFSVCSLIMIVSSFLHT
                     FESNFTKGFSFGSGIALFAGSFYFLVITFSEKRFQHYYTKVFDERNQFIANMSCRLLF
                     LLSVLVLVVLCLINVWFGITFSYAKFLLLILSFVLYGYLLIYFILQKLL"
     gene            complement(38349..38486)
                     /locus_tag="VBC74_000266"
     CDS             complement(38349..38486)
                     /locus_tag="VBC74_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941249.1"
                     /translation="MSRMWTIDFCPPIVYQQYVFISTISISIAMSIFAIYRYASLKKT
                     I"
     gene            complement(38545..38751)
                     /locus_tag="VBC74_000267"
     CDS             complement(38545..38751)
                     /locus_tag="VBC74_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6941250.1"
                     /translation="MKNSLQKLRKSRKLSQAELAVALGVTRQTIISLEKEKYTASLEL
                     AFKIARYFDKQIEEVFIYTESEGG"
     gene            complement(38902..40095)
                     /locus_tag="VBC74_000268"
     CDS             complement(38902..40095)
                     /locus_tag="VBC74_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEO6941251.1"
                     /translation="MSKTIAINAGSSSLKWQLYEMPEEKVVAKGIIERIGLKDSISTV
                     KFDDKKDEQILDIVDHTQAVKILLEDLTKHGIIKDFNEITGVGHRVVAGGEYFKESAL
                     VDDKVVEQVEELSALAPLHNPAAAAGIRAFREILPDITSVCVFDTAFHTTMQPHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHQYVAQEAAKQLGRPLEELKLITAHVGNGVSITANYH
                     GQSIDTSMGFTPLAGPMMGTRSGDIDPAIIPYLVANDPELEDAAAVVNMLNKQSGLLG
                     VSGTSSDMRDIEAGLQSKDPNAVLAYNVFIDRIKKFIGQYLAVLNGADAIIFTAGMGE
                     NAPLMRQDVIAGLSWFGIELDPEKNVFGYFGDITKPDSKVKVLVIPTDEELMIARDVE
                     RLKAK"
     gene            complement(40127..41101)
                     /locus_tag="VBC74_000269"
     CDS             complement(40127..41101)
                     /locus_tag="VBC74_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6941252.1"
                     /translation="MNFEKIETAYELILENIQTIENQLKTHIYDALIEQNSYYLGSSC
                     DLDIVVVNNQKLRQLDLSQEEWRRTFQFIFIKSAQTEQLQANHQFTPDSIGFILLFLL
                     EELTSQETVDVLEIGSGTGNLAQTLLNNSSKELNYMGIEVDDLLIDLSASIAEIIGSS
                     AQFIQEDAVRPQILKESDVIISDLPVGYYPNDDIAKRYAVSSSKEHTYAHHLLMEQSL
                     KYLKKDGIAIFLAPENLLTSPQSDLLKEWLKGYADVIAVLTLPETIFGSRQNAKSIFV
                     LKKQAEQKPETFVYPLTDLQNRENMANFIENFQKWSRENSHYSKNMIE"
     gene            complement(41216..41587)
                     /locus_tag="VBC74_000270"
     CDS             complement(41216..41587)
                     /locus_tag="VBC74_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGG"
                     /protein_id="HEO6941253.1"
                     /translation="MILKKKLKAGILLQAIVLAAVFTLVLQFYLARILATERQYHSQI
                     EASKAYLTAQLAYKTIEGDSISGKCYFTGGYASYLQEGNYLQVKVTLDKGGNYNHKFY
                     RKIDTLQVPLKSNDNKASHKH"
     gene            complement(41565..41957)
                     /locus_tag="VBC74_000271"
     CDS             complement(41565..41957)
                     /locus_tag="VBC74_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEO6941254.1"
                     /translation="MVALVTITGALLVYQGLTKLLAQQIVVMSSSSQSEWVLLTQQLN
                     AEFEGAHLEYLRQNKLYLRKQDKIVTFGKSNKDDFRKTGYNGRGYQPMVYGLDNCQMS
                     QTKSMVKLVFYFKDGLKRTFYYDFKEET"
     gene            complement(41946..42021)
                     /locus_tag="VBC74_000272"
                     /pseudo
     CDS             complement(41946..42021)
                     /locus_tag="VBC74_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793382.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
     gene            complement(42018..43109)
                     /locus_tag="VBC74_000273"
     CDS             complement(42018..43109)
                     /locus_tag="VBC74_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEO6941255.1"
                     /translation="MTLRQVTLKNTHQTSGIDKWISWLKKDISVRNRHKSKKLSLKKQ
                     RKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDCYTDRMNKALLEGKDLSKMLGELGFS
                     DTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLSFLVLIMIGL
                     RHYLMPQLGENNFATRLITNVPNIFLLLLAVVLIFSLIFYIIQKRLSRIKVACFLTTI
                     PLVGSYVKLYLTAYYAREWGNLLSQGVELDQIVKVMQNQKSKLFREIGYDMEEGFLSG
                     KAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQP
                     VIFIFVALIIVMIYAAMLLPMYQNMEILS"
     gene            complement(42994..43926)
                     /gene="tadA"
                     /locus_tag="VBC74_000274"
                     /pseudo
     CDS             complement(42994..43926)
                     /gene="tadA"
                     /locus_tag="VBC74_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            complement(44098..44463)
                     /locus_tag="VBC74_000275"
     CDS             complement(44098..44463)
                     /locus_tag="VBC74_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEO6941256.1"
                     /translation="MYQVVKMFGDWEPWWFIEGWEEDITEIAEYDTLSEALLYFQEEW
                     DRGQEKWPYFQSKSSLLATFWSIKEKRWCEECDEYLQQYHSLMLLKEWQEIPKEESIE
                     RFEVFNKIAELPSACSLNL"
     gene            complement(44577..48227)
                     /gene="rpoC"
                     /locus_tag="VBC74_000276"
     CDS             complement(44577..48227)
                     /gene="rpoC"
                     /locus_tag="VBC74_000276"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEO6941257.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIICDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPMDTPLEPKSLLTEREYREKLQ
                     EYGYGSFVAKMGAEAIQDLLKRVDLDAEIAVLKEELKSATGQKRVKAVRRLDVLDAFK
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDLAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDHDEAVMAYQNGYVHLHTRVGIAVDSMPNKPWTEEQKHKIMVTTVGKI
                     LFNDIMPEDLPYLIEPNNANLTEKTPDKYFLEPGQDIQAVIDNLEINIPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     DINKAFRRGLMTEEDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLTITAITDGKEVTETLEERLIGRYTKKSIKHP
                     ETGEILVGADTLITEDMAAKVVKAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGEVVAIEEDSSTRTKKVFVKGQTGEGEYVVPFTARMKVEVGDEVARGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPLAVNEVEIIEGTPVDA
                     EVTEVSTPTTED"
     gene            complement(48344..51919)
                     /gene="rpoB"
                     /locus_tag="VBC74_000277"
     CDS             complement(48344..51919)
                     /gene="rpoB"
                     /locus_tag="VBC74_000277"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO6941258.1"
                     /translation="MAGHEVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDAG
                     LKEVFEDVLPISNFTDTMDLEFVGYELKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDAKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGDSELVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNLKTRLLNQTIAENLVDGETGEILVEAGTVMTRDVIDSIAEHIDGDLNKF
                     VYTPNDYAVVTEPVILQKFKVVAPTDPDRVVTIVGNSNPEDKVRALTPADILAEMSYF
                     LNLAEGIGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRSTGAVTNEIV
                     WLTADEEDEFTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFPSSIVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAKH
                     DGRVIFSDAEKVEVRREDGSLDVYHVQKFRRSNSGTAYNQRTLVKVGDLVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEDSLRDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGVVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWETVQEAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDVMHVDDLEKARVKQEAEEKQAEQVSEVVQED"
     gene            complement(52443..54740)
                     /locus_tag="VBC74_000278"
     CDS             complement(52443..54740)
                     /locus_tag="VBC74_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6941259.1"
                     /translation="MFKGNKKLNSSKLGDYTPLEFGSIFLRIVKLLSDFIYVIILLFV
                     MLGVGLAVGYLASQVDSVKVPSKNSLVTQVNTLTRVSRLTYSDKSQISEIATDLQRTP
                     VAKDAISDNIKKAIIATEDENFNDHKGVVPKAVLRAAAGSVLGFGESSGGSTLTQQLL
                     KQQILGDDPSFKRKSKEIIYALALERYMDKDSILSDYLNVSPFGRNNKGQNIAGIEEA
                     AQGIFGVSAKDLTIPQAAFLAGLPQSPIVYSPYTADAQLKSDKDLSFGIKRQKNVLYN
                     MYRTRALTKDEYKSYKDYDIKKDFIKPAVATTNHHDYLYYSALSEAQKVMYNYLIKKD
                     NVSEHDLKNDETRATYRHRAIEEIQQGGYTIKTTINKSVYQAMQDAAAQYGGLLDDGT
                     GKVQMGNVLTDNSSGAIIGFIGGRNYSENQNNHAFDTARSPGSSIKPILPYGIAIDQG
                     MLGSGSVLSNYPTTYSSGEKIMHADEEGTAMVNLQESLDISWNIPAFWTYKMLRDRGV
                     DVKNYMEKLDYPIENFGIESLPLGGGIDTSVAQQTNLYQMIANGGVYHKQYMIESIED
                     SNGKVIYNHESKPVRVFSKATATILQQLLHGPINSGKTTTFKNRLQGLNSGLAGVDWI
                     GKTGTTNSTSDVWLMLSTPKVTLGGWAGHDNNASLAKLTGYNNNANYMAHLVNAINNA
                     DGNTFGKSERFRLDDSVIKAKVLKSTGLQPGVVTVNGRRITVGGESTTSYWAKNGPGT
                     MTYRFAIGGTDSDYQKAWSTLGGKR"
     gene            54851..56110
                     /locus_tag="VBC74_000279"
     CDS             54851..56110
                     /locus_tag="VBC74_000279"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEO6941260.1"
                     /translation="MNIFDELKERGLVFQTTDEDALRKALEEGSVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDVERSLQTKKTVVSWGNKIR
                     GQLSNFLEFETGDNKAVLVNNYDWFSNISFIDFLRDVGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFYELNKNYNVTLQIGGSDQWGNMTAGTELIRRKSNGVSHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDADAVRFLKIFTFLSLKE
                     IEDIRIQFEEAPHQRLAQKTLAREVVTLVHGEKAYKEAVNITEQLFAGNIKGLSVKEL
                     KQGLRGVPNYHVQTEDNLNIIDLLVTSGVVNSKRQAREDVSNGAIYINGDRIQDLEYT
                     ISENDKLENEITVIRRGKKKYFVLNFK"
BASE COUNT        17922 a        11401 c         9001 g        17817 t
ORIGIN      
        1 aagtagtaat agggcaacta ttagtagtat tattttagaa ctcttttgca ttttcactcc
       61 tttatgaaac aattacatca tctagctata atatatcaac ttatgaattt gaataagcca
      121 attatttatt aatttgtgag gcaatatctg agtctgtttg ttcgacaaca tccgcaactt
      181 tcaataattg ttgatttata tcttctaata attgtgcaaa ttgtgtgatt tttggagata
      241 attcattgaa ttgggcttca aagctatcaa atgctgtacc atcccaattt tcatcaataa
      301 cagcttgttc ttgagtcaaa actgttaaca catctgtaat tgattgtgat cctgttgtat
      361 atttttgtgc tgaaatacgg agttcttcag gtgttagttt aatttgtgac atcatttgct
      421 ccctattttt ttaactgcag tatagcatat ttagatgttc cttacacaga taaaactcat
      481 aataggttaa tctattaaaa atacaaacag cgatttatat tacatctact ttactttcaa
      541 aacggaaagg ttatcaaaag attttaaaaa aacaccctac aatatcttgc aaagtgcttt
      601 gaaacgttga tataacagta tattaacatt ttgagaattg tgatgcttta cgggcttttt
      661 taagacctgg ttttctctga caactgtaat atatggtgtt atatagtata aactagctaa
      721 tactcatcaa tttacatcca aaaagtgtac tatgtattgc aatatattcg cgaaggggtt
      781 atcaggttat caaaagtaaa attgacaata aaaaaacacc ttgcaattgc ttgcaaagtg
      841 ctttgaaacg ttgatataac agtatattaa cgttttgaaa attgtgatgc tttacgtgct
      901 tttttaagac ctggtttttt acgttcaacc atacgtgcgt cacgtgtaag aagtccagca
      961 cgtttcaatg aatcgcggaa atctgggtca acttctaaaa gtgcacgtga gataccatga
     1021 cggatcgcac ctgattgacc tgcatatcca ccacctacaa cgttaacaaa aacatcgtat
     1081 gaaccttgag ttgaagtaac tgcgaaaggt tggttgataa caagacgaag gtctgcgtgt
     1141 gggatgtact cttctacatc ttttttattg attgtaattt taccagtacc tggaaccaaa
     1201 cgaacgcgtg caacagcgtt tttacggcga ccagtacctg catattgtgc ttgtgccatt
     1261 atataatgct cctttcctct tagataagtc ctgagatatc aagtacttct ggttgttgtg
     1321 cagcatgtgt atgctcaccg ccaacaaata ctttcaattt cataccttgt gcacgtccaa
     1381 gagtgttatg tggaagcatg cctttaactg atttttcaat caaacgaaca gcatttttag
     1441 aacgaagttc acctgctgag atttgtttca aaccacctgg atacattgag tgagtgtagt
     1501 agattttatc tgatgctttt ttaccagtta atttaacttt ttcagcattg ataacaatca
     1561 caaagtcacc tgtatcagtg tgtggtgtga aagttggttt gttttttccg cgaagtacgc
     1621 tagcaactac tgcagaaaga cgtccaagag gtacatctgt tgcgtcaaca acgtaccatt
     1681 tgcgttcaac ttggcctggt ttagccatga aagttgtttt gttcatgatt tctcctatac
     1741 gaattcgttt ttgtttacag gtgtggatgt tccggtccac gagagtattt gaaaggttcc
     1801 ggggccttac aaatggggta aacaataccg cttataatca tatcaaaata aggtagcatt
     1861 cgtcaagtta tttcttttat tttatcaatt ttcatcccaa aataaatcat ctacagtttt
     1921 attcaaagct tgacacaatt taatcgctta attatagcct cctttttcaa tggcggctat
     1981 tgtctgacat gatacaccga ccttttcagc tagagactgt tgtaataaat ccatactagc
     2041 tctagctgtc tttaagcgta aatttttcat attactatct gtccttatac ttaaggtagt
     2101 atactgaagt tcccatagct atcaatgata ttcctataac tgctaaaatt gcatcatttc
     2161 cccttgaaaa ataagtagca atatcaactt cctagtaaaa gcatcaataa aatagttatt
     2221 cctatgaggg caaatgcgcc agcaacaaaa acattttcca aaatctttca tcaacaaacg
     2281 gagagactct ttttagatta gaagtcgtaa caaaatagaa ataccactaa ataaagttaa
     2341 tattattata aaatcaggtg acacgcttcc ctttattagc tttccaaaaa cactttcaag
     2401 ccataagata aaaaacatcg tctaaaaaca tttttgataa ctttgtaaaa ccaattgttg
     2461 ttgtcgttca tcatattgag gatagttctt attttatttt tcataatatg tctcctacta
     2521 ttttatagtt tcatagcatc atataaaata cattttataa aatgtatact aaaagaacat
     2581 aacgtactgt tatgcccttt gatttgttat cggttttctt cccctaaaga gaatagagct
     2641 aaagcacctg agcccacatg agctgaaata actggcccta atggacgaat aataacttct
     2701 tcaatacgct catctgctaa taactgttct ttcataactt gagctgaatc tttctctcca
     2761 ctataagcaa taattaaagt gctataagat agtgtttttg ttgcctgagt cacaatctct
     2821 ttaatacctt ttttacggcc ccttgtcttg gcaaatggta ctaatttacc ctcactatct
     2881 aacttaagta atggtttaat cttagcaaca cttccaatga tagctgcacc ttttgataag
     2941 cgaccactac gcattaaatg gttgagatcg tcaactaaaa agtatgtgtt taatttaggg
     3001 agaagtgatt caattttttg tttcacttct tctaaagact taccttcttg tcgctcttta
     3061 gttgctaaca tcgctaagac accctcacca catgaagctg ccattgtatc cacaatctca
     3121 atttgagcat caggatattt gtctaagacc atctcacgcg caatagttgc actctggtaa
     3181 gtccctgata aatgtgaaga tagcgctaaa tataacaagg catggtcatt ttctgcatag
     3241 gttgaaaaaa cttcttcaaa ctgaccaacg ttaatttggc ttgttgtagg ctttgctccc
     3301 tcttgcatac gctccaacaa gaagtcacta gtaatttttt cgtctccgac tgtctcataa
     3361 gtctttccat caagttcaat ggttaaacct ataatatcga cattgtgctc ttgagcccac
     3421 ttttcatcta aatccgatgt cgaatccgtt agtattttaa aagtcattgt tacctcaatc
     3481 tattattttt atttgataat caaagtagtt ccaatattat atcaaaaaaa ttgttggctg
     3541 gctaataaag tgttagtttt tacataccat ctaaaaaatc cttgagtttt cctagttgtt
     3601 catcattcca cggccttaac tttggcgtac tcaaatcgat ttgactggac atcttatcca
     3661 aatctgattt tactgtcgct acacgctgtt ccaattctct agctgagaca cgtaaagcac
     3721 cctgagaaaa tatagtccat tgttcattta aatcactggt agcaacggag actaaattta
     3781 acacagattg attaagttct gccgctgctc gttcaatata gctatccgca gtttcgtcct
     3841 cctcagtaaa gataacggat attttgtatt ggtcataacg ttgacgtaca ccaggaacat
     3901 actgggcatc aaatacacaa ataatatcaa tgtgttcgaa atgtgcgtaa tgatttagct
     3961 ttctaagtaa tacttccctt gcctcttcta aacgattgct tttaaaaagc tgtcgagtat
     4021 ctttccaaaa agcaatcata ttgtagccat caacgagtaa aatagaatgt tttttcatag
     4081 gcggtttcta aatacttcat acatcaaaat agcagctgcg acactggcat ttaaactctg
     4141 aacatgacca ttcatgggaa ttgtaatcat ttcatctact tgctttttga tattatgaga
     4201 aatacctttt ccttcattac caataactag ggctaattta cctttggtat tccacttatg
     4261 cgatggtgtc ccattcatat cagtgccaaa tatccagaag tccttatctt ttaaagtatc
     4321 caaagtttga ctgagattag ttacccttgc gataggaaca tgttcaacag ctcctgtaga
     4381 tgtctttgaa accaccgggg tcactccaac agaacgatgt ttggggatga taatgcctgt
     4441 tacattggtt gcatcagctg tgcgtagaat agaacctagg ttatgagggt cggtcagacc
     4501 atcaaggatt aaaattagag gattttcttc attctctgcc tttgtcatta tctctgataa
     4561 atccgcatag gcaaattccg aaacttttag aacgaatcct tggtgaactc caccatttgt
     4621 catgtctgat aagatttttt taggagtcca ggaaatggat actttttttt cagtcgctag
     4681 tgctttgact ttatcaacgt tctttcctct taagtcatct tgcaagtata atttattacc
     4741 agtattagct cttaaactct ctgtcacagc atgtaagcca tagacaaggt cacttgattc
     4801 ttctttaaat tgtttatctt tcatagtcat attatatcat gaatgctata gcataaagct
     4861 ttttatagca atattttacc aacaaatcct aatgtctcta taaaaaagag aggatatagc
     4921 ctctcttttt gtttattttt caatggtttc aatacaccac tgtattagag tttccaagcg
     4981 ctttatctga ccagtcatat ccaagtaccc cataagagcc tcaaaacctg tagacatacg
     5041 ataggtaata atatcagcat ttttagcttt tgtatgacta ttcgcattac gtcctcgttt
     5101 ataaattaac tgctcttcat cagttaaaat attttcttct aacatggcat taattaacaa
     5161 tgcttgggca ttagcagaaa catattgggt tgctttccga tgtaactgat taggtttggt
     5221 aaaaccctgc ataattaaat ggcgtcggat atacaaagaa taaactgcat ctccttcaaa
     5281 agctaaagca ataccattaa tcaaacgaac atcaatcacg agtccacctc acgccatcct
     5341 tagtatctaa caatttaatt ccttgttttg ctaattcatc tctaatacga tcagcagttg
     5401 caaaatcacg attagctcgt gcttcttgac gttgttctat taaagattca atatcagcat
     5461 caagaacttc ctcttggaat acaataccaa aaatttctaa taattcagca agggtttcct
     5521 taacttttga agtatagtgt cctgaattaa tccattttgc catttcaaag ataacagtaa
     5581 taccgtttgc tgtattaaaa tcatcatcca tcgcttcttg aaatgctttc acaaattgtt
     5641 ctaattcttc atcattagca ttctcttgga ttggtaagtt gaaagtattt tttaaatatt
     5701 taaggttaac ttccgcatca tgaacggcct tttcagtgaa attcacgggt ttacgatact
     5761 gttgtgtcgc taagaaaaaa cgtattactt gtccgtcaac gctcttcaac atatcgtgaa
     5821 cagtaataaa gttaccaaga gatttagaca ttttctcgtt atcaacgtta acaaaaccat
     5881 tatgcatcca gtaatttgca aaggtcttac ctgttttagc ttcagattgt gcaatctcat
     5941 tcgtgtgatg tggaaattct aaatcagctc ctccaccatg gatatcaata gtatcaccaa
     6001 ggatctcagt tgccataaca gaacattcaa tatgccaccc tgggcgccct ttcccccaag
     6061 gactttccca tgagacttcc ccagatttag ctgatttcca aagtgcaaaa tctaaaggat
     6121 tctctttaat ctctccttcc ccatcaacac gaccacttgc accaatctct aaatcttcca
     6181 aagttttatt ggctaatttt gcataatgat gtgatttact tactctaaaa taaacatctc
     6241 cattagcttc ataggcaaat tccttatcaa ctaaaacttt aacaaaatca ataatctcat
     6301 ccatatagtc aataactcga ggattttttg tagcaggttt gacacctaat gcagcaacat
     6361 cttccataaa tgctgaaata aacttatctg aaaaggattt tgtatccata cctgcttcag
     6421 ctgcaccttt tataatttta tcatcaacat cagtaaagtt agaaatataa ttgacttggt
     6481 aaccacaata ctcaaaataa cgacgaatag tatcaaaagc tactacactg cgggcattcc
     6541 cgatgtgtat atagttataa actgttggcc cacagacgta catgttgact ttaccttcat
     6601 tgagaggtat aaaatcttgt aaactgcgag tcatagtgtc gtaaattttt atcatggttt
     6661 ttcctttttc tgtttttgat aagctttata caaacgaaca atatacatta aagcaaaggc
     6721 agcccaaata attcctaaga gagagttacc ttgtgcaaac tttaaatagc ttaaacaagc
     6781 tcctgctagt atacctaaaa ttgataatat ttgagttgtt acataatcct tcacaaatac
     6841 ctcctaaagt ttggacgaat aataactttc ttctcgatca tgttcaatac ttcggatttg
     6901 aagatcatcc ttttgaccat ggacacggac aactttagct ggaacaccaa ctactgttac
     6961 gtctgctggc acatctgcaa ggacaactgc tgcagcaccg actttagcat tttctccaac
     7021 ctcaattgga ccgatgattt gagaatgtgc tgaaattagc gcacctttac gtatggttgg
     7081 gtgtcgtttg cctttgtctt tacctgtgcc acccaatgta accccatggt atagcatagc
     7141 acctttctct acaatggcag tttctccaat gaccaatcct gaaccgtggt caataaaaac
     7201 cccttccgaa atcgtcgctc cgggatgaat ttcaatttgt gtccagaaac gccaaaattg
     7261 actgtgcata cgtgctaaca atttaaaatt atgattccat aaaaaatgag atagcctatg
     7321 agctgcaaga gcttttattc ctggataagt taaaataact tctagagaac tacgagcagc
     7381 tggatcttgt tctttgacaa tagctatact ttctttccac caccccatag ttttcctcaa
     7441 tcgttttcta catcagacca atcaactaaa acttgttgtt catcaaatgg attgatggct
     7501 actccaaagg tatcatcaat ctcattttct ccattacgcg gtttataaat atcgtcaatc
     7561 gtccaggcta aaattactcc ttgtgctttc ctaaaagcac caccaaaatc attatggtta
     7621 taccattttt caaaacttaa aaggtttgag aatactggaa cataactttt tccttcgggg
     7681 gtagacattg tagggaaaaa acggtcaaaa ctaccttctt cacgtggatg gacaaaggca
     7741 ggtaccaagt aggttttatc catgatatcg gcaacaatgt tatcttcatt taacaactga
     7801 ttcaagattg tggtgtaatt gttcataaat tgcaccatat cttcacagta gaaaatagtc
     7861 gagttcccaa aatcgccttc tttttttaaa ttatagacca atcctgctaa gccatgtgaa
     7921 atagcttcat caagaacgtc taaactacga cgactctccc aaaacatatc tcgagcactt
     7981 tcttgctcct ctttgaaatg attaaggtca gtaatatccg taaaaacagg aacaacttct
     8041 tgaccatcta cttgaatagc atagggttcc ttgcttgccc aaaccgtaga gtggtgcaag
     8101 gcattcacaa gacctatact atctaaaaaa ttatccggtg cattgataaa agcacgcaat
     8161 ctaatgtcta actcgtttgt acttgtcata ttaagattcc tttttaggat tgtcagcttt
     8221 tggaggacgt ggtaataatg ctttcataga ggcatcaact cgccctttat catcaatttt
     8281 gatgactttc acatcaactt cttcaccaat ttcaagaaca tcagctacat ttgctgtacg
     8341 tgtccatgca atttctgaaa tatgtaccaa tgcatctgtt ttatcaaaga ggttgacaaa
     8401 ggcaccgaat ttctcgatac gaactacttt agcatggtaa acttctccca cttttgcttc
     8461 acgtactaaa cttgcaataa tgtctttcgt acgatcaata gcagcctgat cactagagaa
     8521 gatagataca tttccttctt catcaatatc aatcttaaca cctgtttcag cgatgatttt
     8581 atcgatagtt tcgccgcctt taccgatgac aactttaatc ttatcaacat cgatttttat
     8641 catatcaatc ttaggtgccg ttggtgctaa ttgtggacgt gactctgcga tagctccatg
     8701 aaggacatca agaatttcaa agcgtgcttt tttagcttgc gcaagagctt cttccaaaat
     8761 ttgaggggta ataccttcaa ttttaatatc catttgaagt gctgtaatcc cttcacgtgt
     8821 acccgcaacc ttaaagtcca tatcgccaaa atggtcttct aatccttgaa tatcagtaag
     8881 gactgtgtag tttgttccat ctgatatgag tcccatggca ataccagcaa ccggagcttt
     8941 tataggcaca ccacctgcca taagagcaag cgttccagca catatagaag cctgagatga
     9001 tgaaccgttt gattctaaaa cttctgcaac tagtcggata gcatacggaa actcctccaa
     9061 gcttggaagg acttgttcta atgcacgctc tcctaaagca ccgtgaccaa tttcacgacg
     9121 ccctgcagct ccataacgtc ccgtctcacc aactgaatat tgtgggaagt tatagtggtg
     9181 cataaagcgt ttcttatact ctggtgttag cccatcaatg atttgtgctt cacccatagg
     9241 tgctaacgtt aatactgata atgcttgggt ttgtcctcgt gtaaagagac ctgatccatg
     9301 aacttgtggt aaaaaatcaa tcactgcatc taatggacga atctcatcca cacgacgtcc
     9361 atcaggacga accttatctt ctgtaatgag gcgacgaact tctgcatgtt ccatttgttc
     9421 taaaatttca gcaacatcac gcataatgcg atcatattct tcatgttcag cgtaacgcgc
     9481 ctcatattca cctataacta cttctttaac tgcttctgta gctgcttctc tagccttttt
     9541 ctcttcaact tgtactgcag cctgtaaagc tatgttatga gtagcaataa tttctgcttg
     9601 aagttctgga tcaacttgaa gaagttctac ttcggctttt tctttaccaa tagcagttac
     9661 aatctcttct tgaaaggcga ttaattcaca gacagcctca tgtcctttaa gaagagcctc
     9721 tagcatgatt tcttctgaaa gttctttcgc tccagattca accatgttaa tcgcttcttt
     9781 agtacctgca accgttaatt ctaacgcaga agcctcttgt tcttgtgctg tcggattaat
     9841 aataaaattg ccatcaacat aagcaacttg aactccagca attggcccat tgaaaggaat
     9901 atcggaaatc gataatgcta atgatgaacc aaacatagca gccattggtg cgctagcatt
     9961 ttcatcaaat gaaagaacag tattgatgac ctggacttcg ttacgaaatc cctcagcaaa
    10021 catcggacgg attggtctat caataagacg tgctgttaag gtagcatccg tacttggacg
    10081 cccttcacgt ttgttaaaac cacccggaaa tttcccagca gcatacattt tttcttcgta
    10141 attgacttga agtgggaaaa aatcacctgt tgacattttc ttagacataa ctgctgctgt
    10201 aagtacagta gaatctccat aacgaacgac aactgatcca tttgcttgtt tagccacttg
    10261 gccagtttca acaactaatt ttttaccagc aaaaatcatt tcaaagactt gttttgacat
    10321 attattctcc atattttttg tttacgcgtt tcctacaaag ttcaatctag ctgttctaaa
    10381 ctcaactttg cataaaacca cgtataagct ttattctaac ataattctat aaaattgagt
    10441 agcgaaaatc cgtgggggaa gctgtattat cattcattaa tgtaaacgta aaaagtctga
    10501 ttattaatac tagcaatcag accttgttac aatgattaaa ataaatttct agggttaatc
    10561 ttagcttgct taataaccct cttcataggt taaaacactt tcttactgtt ggccccctca
    10621 aagtacttac gttcatagta cgtttaaaac aaaaaacact agaaaatttt ctagcgtttt
    10681 tacttgactc tgttgtctat taacgacgaa gtccaagtga ttgaatcaat tcacggtaac
    10741 ggttaacatc tgtacgacgt aggtatgcta ataagttacg acggtgacca atttttttca
    10801 tcaatccacg gtaagtagcg tggtcttttt tgtgttgttt gatgtggtca ttaaggtggt
    10861 tgatttccca agtaagaact gctacttgaa cttcaactga tcctgtatca ccttcgtgac
    10921 gtgcatattg agcaatgatt tcattttttt tctcttttga gattgccata atatattctc
    10981 cttttattgc ttcatccgag tcttaggatt ggcactccta aaaccaagaa aaagttggtt
    11041 tgtcttatag accctcttat tttatcaaat tagaataaac tttgtcaaaa gtttttaata
    11101 gaaatatgga taaccaactg tctctaaagg aaaatccttc atagactaaa cgagactttg
    11161 catctttttg ccgaaagacc ttagcataag tagatgacat actagttggc ccacacataa
    11221 atatcgttgg ttgtccttca aaaacagact gactaaaatc aagtcttcct tggaggctac
    11281 tattatttaa gtgaagttta aaattaggat tggctttagc ataggattct aacatatctt
    11341 gataaatcaa attgtcttga tttgaaaatg tataaaagaa gtcaactctc ttagtcaaaa
    11401 tactattttc tctaataaat gaaatgaagg gagtgatgcc gatacctcct gcaatccaaa
    11461 cctgttcttt cttgtcttta tcaaataaca tatgaccata agctctatct aaggctattt
    11521 ttgtcccaac ttttagctgt ttgtaaatgg atttcgtata atctcctgaa gctttaacag
    11581 ttaaaaatat cactctatca tgaccacccg atatcgagaa aggatgagct gctgattcaa
    11641 agccggcttg ataaattttt aaaaaagtaa attgtccata gtcatatcga taaggacgct
    11701 tcattgctat ttcaatctca gttgtatcat ggttaagatg agtaaccttt tgaacgtacc
    11761 ctacacgacg aaaacgcatt ctagaatata ggaaaataat ataaaatcca gatataaccc
    11821 caatgacagc ataacctaat acgattaaac tgagtaaagt attccctagt atcctatctc
    11881 ctaaaatcag aaaagtatgt accaatccca aaatatagac taaataaaca aagcgatgaa
    11941 taaaacgcca aatttcatac tttaaaaaat ttccgaaata agctacaaaa acaatactta
    12001 gaaaaagata taatcccgca ctgccaatag tctttgcaaa ctcacttcca tgtccgcctt
    12061 gtacaagacc aattttgtgc aatagaagca aaatcattga aaatacagcc attaccttgt
    12121 ggtaaaaata catattttca ataccttgaa agtaactttc aagtatccta aatttggttg
    12181 ataagaggaa tgttagcgat aaacttgtca aagctaatcc tggtacaaca aattcactta
    12241 atcctgcatt ttgccatgct actagtgtca ccactatgct agataaaatt aaaacaatac
    12301 ctttccaagc tcgcataacg accacccctt tatttttagt aaggataaaa taccacaaga
    12361 tacttataca aaacttaaaa atttcagaaa ataacaaaaa ggcaactcca atgttgcctt
    12421 tttgttatga tttataaatt gatttgcaat gctctactat atctttaaca gcgagtccat
    12481 actcctctaa taaatattcc atctgaccta cctgaccaaa tcgctcatca atacccatac
    12541 gactaacagt agtgtctttc tccatactaa gccattcgca taatgcactt ccgataccgc
    12601 caatacggtt atgattctca atagttacaa tggattgatc tatcagtaat ggtttcagtt
    12661 cttctggaag gggcttaatt ttaaaaaggt cgataactga ggcctcaatc cctagttctt
    12721 tgaggtagtc ggctgcttct atcgctctag aaaccataat accacttgct acaagagcaa
    12781 tatcttttcc ctggcgtaat tgtataaaac cttttgaaaa atcttcgcat ccctcataaa
    12841 ctgcagtggg ggccttacga atagttctaa tgtattttag accatcaata cttaatgttt
    12901 gttttaaaat tgcttcaaac tgaatatcat cactaacctc aaaaatagtc gcctttggaa
    12961 ttaaccttag tagacctaac tcttcaaaag gcatatgagt tccgccattc atttctgcgc
    13021 taactcctgc atctgagcca ataatcgtag ctgataattg tgagtaacct aacgataaaa
    13081 atacttgatc aaagacacgc cttgacgcga aaggaccaaa agtatgaagg tatggcttgt
    13141 aacctttaat ggcgagccct gctgcaagac ctaccatctc agcctccata atacctagat
    13201 taatataacg ttttccaaat tctgatgcca gtacatttgt tgacattgaa ctcgacaaat
    13261 cggcttctaa aacggtaatt tgtttatttt cttgattagc ttgaaggagg aaatcacgat
    13321 agacaagcct catttctttg gttgaacatc tcattctacc acctccaaac tttctctcaa
    13381 gcttatcacc gctcgctcta acattgtttt ttgttgtaaa tctggtcgta aatgatgatt
    13441 agaagctaac tcctccaact ctttcacacc ttgtcccttg atactatcta aaacaatgca
    13501 ttttggtttg acactatttg aatcttgaaa agttttaata gctttgtcaa ttgcctcaat
    13561 gtcatctcct tttacacgta ctgcatcaaa tccaaaagct tcaaatttag caacaaagtc
    13621 tccaggatta caaatatcag ctgtcaaacc atctaattgt tttttattat catctacaaa
    13681 aacaattaaa tggtgcagtt gatgatgcgc agcaaattgt atagcctccc aacattgtcc
    13741 ttcatttaat tcaccatcac ctacaatagt ataagtataa tagcttgaat tctcaatctt
    13801 ttgagcataa gcaattcccg ttgcaatact aatgccttga cctaatgagc ctgtcgttac
    13861 atctatacca ggagttaaat tgcggtcagg atgcgaaggt agtttggtac cgtttgtatt
    13921 gagcgaatga aggaatgttt tatcaaaaaa accttttaaa tagagtgtac tatacaaggc
    13981 tggaccagca tgtccttttg ataagaccat ataattccga tcactttctt tgaatttttc
    14041 agggtttata tccataatat caccgtacag aatagccaaa gtctcaacaa ttgataaact
    14101 acccccatag tgaccgaatc ctaaatggtt tagcgtttct agtgtattta aacgaatctc
    14161 cgttgcaaat cgtcttagag atgaatatct ttcttctggt gatgtcatta ttaaccttcc
    14221 ttatgggttg attttccaac aaatgaaatg ccaaaaagtc caattaagat aactacaata
    14281 ccaataacaa tagctatcgc accgccgaca tgattaagag cgccaagaat gataccggtc
    14341 aaaccaaaat cagcatctga gaatgttgaa cctttgaaac caagtccacc tagtacaggc
    14401 atcaagaaaa tcggcaagaa actgatgagg ataccttgaa caaatgcacc aatagtcgct
    14461 cctctcacac caccagaagc attaccgata acacctgccg ttgccccaca gaagaaatga
    14521 ggaacgaccc ctggtaaaat gacagtagtg ccagtcacaa tcataactat catactaacc
    14581 aatccaccaa caaaacttga gataaaacca atcaatactg cattaggtgc ataagggtaa
    14641 acaattggac aatccaaggc tggttttgaa tttggaacca atttttctga gattccctta
    14701 aaggctggta caatttcacc taggataagg cgtacacctg ctaaaataac gaatacacct
    14761 gctgcaaatt gcccagctag ttggagggca taaacaagac cattgacacc gttacttatt
    14821 tcttttgcaa tataagcctc accagcaaac actgcaacga taaggtaaat aattgccatt
    14881 gagatagtaa tacttacggt actgtcacgt aaaaatgata aactctttgg aaatttaata
    14941 tcttctgttg attttgattt atctccaaca attccaccaa taaatccact caaccaatag
    15001 cctaaagaac caaagtgccc aagtgccacc ttatcattac ctgtcagttg aatcatgtat
    15061 ttttgcacaa atgcaggaga tacgctcata ataatcccta acgctagact tccaaaaagg
    15121 atgagcgaga acgaagtaaa gcctgctacg gcaaaaataa cagcaatcat acaagccata
    15181 tacaatgtat gatgtcctgt cagaaaaata tatttaaatt ttgtaaaacg agcaattaaa
    15241 atattaaaaa tcattcccgc taacataatc aaagcagttg ctgagccata cttcgtaaga
    15301 gctactgcta caatggcttc attattagga actaccccca ccaaatgaaa agcatgttca
    15361 aacatttttc caaatggatt caaggaattt tggactatcc ctgcacctcc agaaaccact
    15421 aagaagccaa caaatgtttt tattccacct tttacaatat caggaacacc ttttttctgc
    15481 agtactaaac cgataatggc tatcaaggcg actaaaatag ctggcgttct ggcaatatta
    15541 actaaaaaat ccaatagacc tttcatggca tcaacctcct agattaagcc tttttcttgg
    15601 caaatacctg tcactaattc tctcaattca tccatatcaa tgatactgtt taaaatacga
    15661 acatccccca ggtgaccagc tgaatcctcc aaatcacgac caacaatcca aacatctgct
    15721 gcgcttggat ctgctccacc taaatcataa tgttcaactt caacatcaga aacccccaaa
    15781 tccttcaaaa tagactcaat attcatttgt accataaaac ttgaacctag acctgatcca
    15841 catgctgttc caattcttaa catctttaat cctcctgttt tataatcatt tcaatatctt
    15901 gatacccacg tgaagctaac aaagctttaa cattattatt atcacgaagt attgttgtca
    15961 aatgcgatag agctttcaaa tgcgtttcat tatcaatagc tgctatgcat aataagagat
    16021 aaatttcctg tttggggtca tccaataaat aaataggctg ttccaaaaca agcattgaca
    16081 taccgacaga gtttacccca tcttctggtc tggcatgtgg aatagcaata cctttaccaa
    16141 gattgataaa aggaccaaac tcttcgacct tttcaatcat agcttctggg taactttccg
    16201 taatttttcc agaagctaac aagggttttg ccgctaaacg aattgcctct ttccaattca
    16261 tttgttcaga acttgtctga taagtttcct ctgttataag ttggtgcaac aagggcctca
    16321 catcctttct aaatatatct tgttttacca ttgtcctcaa agccaatttt aattcctctt
    16381 cacaatgaac atgagcgtat ttttttatag ttgctattaa ttgatcaagt tggaaatcgt
    16441 caaggcaagc tttgggaaaa tctgaaataa ccagttcttt gagctgttgt acttgctctg
    16501 ctgtcatcat cagcgaaact agataattag gtttttttgt ttctacaaat atagtagaaa
    16561 agaccatatc gtaagtttga ttgtcaatca gtttcagctg ctcaatttta gagacacgat
    16621 gaaaatgaat ttgaggaaat aatccacgca atttttcctt aatcactaag gatgaactaa
    16681 cgccgtttgg acagagtatc aaagctttat aagacattga ttgtgtatca cctgattgtc
    16741 taagatagcc tccaaagtga atcacgaagt aagaaatttc actatcgggt attaatccta
    16801 cttgctcttc caaaggacgt agtgcctttt taaccaatag aaataaatca ctgtaatgct
    16861 ctttaatatt ttgtgtataa ccactatcgc cagttaaacc atatttcaat cggaaataag
    16921 ctggaataat atgacgcttc agaccttgta ataactcttc tttattggaa aaattaagaa
    16981 gtgaaacagt ttccatctca tcaacgattg cttttgccaa tgcttcaaag aaatcatcat
    17041 ctttagttcc ctcaccttca aaacagccag ataagatcaa ggtaatataa tctttttctt
    17101 tctgatctaa tggtttagat aaggaaatat cctgacttaa attagtcaca ataatagtcg
    17161 ttaactcttt taattgttca gaaacaatag gagaaccctg caaaacacga tctactgacc
    17221 tttgataacg acacagtatt aaaatgataa aatataaaga tttctcaagc ctatcttgta
    17281 ttggagaaag ttgaaaagat tcatagaaat attctaccgt cttttctaac ttctcatatg
    17341 agagagcgaa tttccaagaa gacaagatat aattcaaaga ccaaaaacca atgggagatt
    17401 ctagtagttg agttatcatc tgaaaagcaa cttgatgctt atccatctca tctcctacta
    17461 gactatatcc ctttgctctt gtataagtga gtgagatacc tctttttgct aatttagaac
    17521 gtagcatctt gatatctgat aaagtagtat tcttacttac tttcaaaaaa tcttgataat
    17581 gcacattgga gacaaactct cttcggcaaa agctataaag gtaaatcaga catacccttt
    17641 cttcctgagt taggtaaaat tgctcagctt ctaataagga gacaatttta tcatgctctt
    17701 tttcatctcc taataactga taacgtccag cagagtgagc aatagctggt aggtgatttt
    17761 ttgctaaaaa actattgaga tagttaattt gctctatgat aacttttatt gaaaactttg
    17821 tttgcactgt taactcatac aaggaaagat ttttccgagc taaaaatacc aacattatca
    17881 caacagtctt attatcaacc atttgttttc ctttctattt ttattttata gtaaccgttt
    17941 acatttttct aggtcatttg agcccaatct ggcactacta tcaattgaat gaatttgggc
    18001 tattgttata gagtaaagaa aaaagaaaga aattgcaaaa tctagagcaa gattccttcc
    18061 cacaagcagc acttaaaaaa cctttactta attgaataag taaaggtttt ttaaatgtta
    18121 tgacactaag atagctaaag tttgataagg ttgcaaagta accgtttcat caagagtaac
    18181 atccttataa tttgatagaa gaacttgtcc ttggagataa ttctccggta attttatctt
    18241 aactttctca gcaaagaaat tatttaaaac aagaagtttt tccttatcaa gatggcgttc
    18301 aaaagcatat actttttcgt tatctttgaa agctgcttta taattcccgt cagctattat
    18361 tggcagttgt ttacgtagcc ttatcaactc ttgatagaaa gtaaatatca gccctgtttt
    18421 ctcctttgcg acattgattt ctttatatga ttttcctact ttcaaccatg gagcgccttc
    18481 agaaaaacct gcattcgtac tatcatccca ttgcattggt actcgagaat tgtctcgaga
    18541 tttcgctcga ataatgctaa atgcctcttc ttgtgacttg ccttcatcaa gcattatttg
    18601 gtaggcattc aaactctcaa tatccacata atcatccata gaactgtaat cgggatcaag
    18661 cattccgatt tcttcaccca tatatatata aggagtacca cgcgataagt ggatagatgc
    18721 tgctaacatg gttgctcctt catttcgaaa acgtttcacg tctacaaaac gatttaatgc
    18781 tcgcggttgg tcatgattat tatagaataa agcattccaa ccatttcctt cgctcattcc
    18841 ttcgccccaa ctatggaaaa ggtcacgtaa tgcaggaaaa tcaaaagcca taatggtcca
    18901 cttttgccca tctttataat ctactttgag gtggtgaaaa ttaaaagcca tggataactc
    18961 ttcacgttct ggtgcagtgt aaagaatgca atttgcaatt gtcgttgaag acatttcacc
    19021 aactgtcatg aaggaatcag cttgtccaaa actagcatta ttcaacatct tgagataatc
    19081 atgtgttatt ggacgatcag tataagctgg ttttccatca ttaattgggc agttctcaag
    19141 tatctcatct ttcccaatta aattaatcac atcaaatcga aaacctttaa cccctttatc
    19201 acgccaaaaa ttgacaactt taaaaagttc cttgcgaaca tcggcattgc gccaattaag
    19261 gtcagcttga gtgatatcaa aaagatgaag ataatacttc cctgtatcac caaatggtga
    19321 ccaagcattt ccaccaaatt tagataccca atctgttgga ttatcacgta agataaagaa
    19381 atcttgatag tagcgatctc ctgcaagtgc ttttttaaac cactcatgtt ctattgaaca
    19441 atgattaagc accatatcaa gcataaaatc tatacggtat tgacgtccca cctcaatcat
    19501 ttcttcaaaa tcgtccatcg ttccaaaatc agggttaatg gctgtataat cagagatatc
    19561 atatccatta tctcgttgtg gacttggata aaatgggttg agccaaacca tatcaatacc
    19621 aagttctgct aaatagggta atttttcgat aataccacga agatcaccaa ccccattacc
    19681 agttgtgtct ttgtatgatt ttggataaat ttgatagaca actttacgtt tatcaatcgt
    19741 catattctgc tcctttccca aaatgctgct gtatttagca gtctaactat aacatcaact
    19801 atcatcaaaa atataacatt tttacagcga tatttgttcc catgagtttt ttcttatgtt
    19861 caaaataatt caaggagcta atgtattaag cccctcaaac aattcctcaa attacctctc
    19921 attttataag agtgctcttt tttaaaaagg atacctaatt acttttaaat tttcttggca
    19981 accattaatg cttgtccacg ttttatctgt ttgggtaaat ctatcaattc ttctggtcgg
    20041 aattcttctt gattagtaat cagaatagga gtttccacaa tgtatccttc ttcttttatc
    20101 attggaatat cgaaagtaat gagtttatct cctactttta cattgtcacc ttgggcaaca
    20161 tgagaggtaa acccttttcc ttctaaatta acagtatcca taccaatatg aataagaaac
    20221 tcaacaccct ctgatgttaa taatccaata gcatgtttcg tagggaaaag aactgaaact
    20281 gtcgcatcga ccggtgaaac tagttctcca tcacttggat cgataaccac tcccttcccc
    20341 ataattcctt gtgaaaagac aggatcacta gcttgatcca gtggtttagc taaacctgat
    20401 aaagggctta ctacactaag ctttgtacct gagacgacag ctttttcttt tgctgatttt
    20461 gtttctgttg ttgaagcaat gacagcatca gatactaatt tttcctcttc agtttttgtc
    20521 aaaatacctg attttttaaa gaatagtgtc aaaaataaag gaataaagat agccactgcc
    20581 atacagataa agaaatatcc catatattta acattaattg acaggaagcc tggaagaccg
    20641 cctacaccaa tagagttagc ttggacatta aaagttgtcg ccaaaagacc tgccacactt
    20701 gaaccaatca taccagcaac aaatggataa atgtatttaa cgtttacacc aaaaagcgcc
    20761 ggctctgtaa caccaaggta ggctgagata gcagctggaa gtgatatttg agcctctttt
    20821 tcatcatgac gatgcataaa ataataagct agtacagctg aaccctgagc aatatttgaa
    20881 agagcaatca tcggccaaag gccagttgta tgtgtttttg tatctgctat gagttgagta
    20941 tcgatagcat ttgtcatgtg gtgcaaacca gtaataacaa atggggcata cagggcacca
    21001 aaaatagcac caaagagcca ttttacagga ccagtcaatc caataagaac gatagcagaa
    21061 atccatttac ctagtgtcca accaataggt ccaaggacag tatgagccaa gataatagct
    21121 ggaactaagg atagaaatgg cacaaaaatc attgaaacaa cttcaggaat atgttttctc
    21181 cagaagattt ctaagtaaga caggctcaaa ccagctaaca gagctggaat aacttgagct
    21241 tgataaccaa ttttttgtac tgtaaagtaa ccaaaattcc aagaccaatt tttagcaatg
    21301 tctgctgctg aagtactagc tacagaataa gcattaagta attgtggcga cactagacag
    21361 atacctagaa caataccgag gatttgagta gtccccatct tacgagtcac agaccataca
    21421 ataccaactg gtaagaaatg aaatatagct tcacctggta accataaaaa agagtctact
    21481 ccgctccaaa acgttgaaac atctactaga gtattccaaa ttggatgccc agaagagtct
    21541 acttgcctga caccatcaac aactttttga cctaaaaatt caaatggaac ggcatctaaa
    21601 atattacgaa aaccaaggat cagaccacca acaataatgg ctggaataat cggtgtaaat
    21661 atttcagcca gcattgtcaa cacacgctgt aaagggtttt gattttttgg ggcagccgat
    21721 tttgccgcct ctttagacac accctcaata ccagaaacgg ctacaaaagc attataaaaa
    21781 atagggacat cattaccaat aatgacttga aattgcccag cattggtaaa ggtacccttg
    21841 actgatggta actcctcaat tacctttacc ttagctttac tactgtcatt caaaacaaat
    21901 ctcatgcgtg tggcacaatg tgtcactgca ctaatatttt ctttaccccc aattgcctct
    21961 aacaaggctt tggcatcatg tttaaattgt tccatctgtt tatccttatc tatttaacgg
    22021 agagtctgtg actccaaggc agaaacttaa agaagactag cttacaatat aattgtaaac
    22081 gattacatat ccctatgcaa atccttagct tatcttttta aaaaatctat aaattataaa
    22141 ataattagtc aataataccc aaaacattag aatttctcac caacgttacc aaaatgttag
    22201 agtcatgagt aggtcagtca cccccaatac tctcttaata aaaaacacct actaattgat
    22261 atagtaggtg agttttttaa tctccattga aagatacgat acgattctct tcaatcatat
    22321 agcattggtt acaaagtttc tctaacatag cgaaatcatg actaataacg atgagaccta
    22381 aatttctatc ctttacaatc tccaacaggc ttttccatac actagcttgt gtaatactat
    22441 ccaacatagt agtcatttca tctgcaataa ggtatttagt ctctggatgt aatgaacgca
    22501 caatcgaaaa gcgttgtaat tcccctcccg agagttcact agggcgacga tttagccatt
    22561 tttcttgaat tccaaaagca tctagtaaat ctcgacttgg gtaataagct tcttctaaac
    22621 tttttttcat aggccataaa ggattcatcg ttttttcagg atgttgttga ataagttgta
    22681 caggtctaaa tgctttgtta ggcaaattac tgccatcaac taatacttca ccagatctag
    22741 gatgtaaaaa acctgcaagc actttggata aactagtttt tccacaacca ctttgtccaa
    22801 atatacctaa aacttgacca ggtgctacct ctaaattaat ctccttaaaa agccattgat
    22861 cttttttatg ataaaagcca agctttttag cctctaaggt catgagtaac tcctttcaaa
    22921 aattcttgct gtgggagaga gcgccataaa cttctagcaa attctgtttg taactgctct
    22981 ccatttccgc taaagaaact agctggagct gtttcaatag cttttccctc tttaaaaata
    23041 gtaatacgat cagcaatttg actagctgct acaatatcat gagtgataaa tataacgctt
    23101 attcctttat ctgcaaagga gcgtagttgg tctaaaacca tttgcagagc atctggatgt
    23161 aatccagggg tgggctcatc cgcaataatc aaagaaacct tatcactaat acacgttgta
    23221 aacaaaacac gtcggagcat tccgccagaa agttggaaag gatacaagtc accatcactt
    23281 tcttttaaac caaactgttg aaacaatcct tcttgagtag cctttgaatt ttctgagata
    23341 cctaagcgca cctgatgttt gactttcata gatggatcta aataattaac tgattgtgga
    23401 atcaacgtaa tatcttttcc tcgcaactgt ttaatgcgtt tagaatttag tgattgacca
    23461 cgataaatca tatctcctgt tacagatgca tttttaggaa gaatatccat aatagcatgt
    23521 gctaataaac tcttccccga accactagca cctataatag ctaataactc acctttttta
    23581 atttctaaat tcagcgcttg tatcggtgtt gattgaaatg gttttaaaaa tcttccgtat
    23641 tgagtgaagg tgatggagag gtctttaatg cttaataatg tttctgtcat atctctacct
    23701 cctaaaaatg atcagtttga gggtaaaaga gtttctttaa agattctccg atagtatcaa
    23761 atgcattgac aaccaaaata agataaagtc ctggaaagat aaccaaccac caatttccaa
    23821 gagagatatg cttagctgcc tctgacagaa tgataccaac cgaaggttgt tcggcagaga
    23881 gcccaaatcc taagaaagtc attgatgctt catgtaggat gacatgtgga aataatagga
    23941 taaaaccaat gaaaatttga gaagcaatca aaggcaggat atgatgcctc acaatataat
    24001 aaggcgtttt tcccatactt ttagaaagtt ggacaaattc tttattcttt agatgataga
    24061 cttcattgcg gataagcctt gctaaagaag gccaatgtgt aacagccgtt gcaatgatga
    24121 ccccttgagc acctttccca acaacaaaag aaatgagaat cataaaaatc aaatgaggca
    24181 taccaataaa caaatcaact aaccatgcta ttattttatc aataatgcta tttcctaaac
    24241 ctgcaagcac tccaaaaact gtcgccagaa tgacccccat aagggcacct aataagccga
    24301 cttgtagaga gaaataaagt cctttaatcg ttctgacaaa catatcccta cctaaaccat
    24361 ctgtcccaaa aagatggttt aatgaaggag caaggttccg taaagctgca ttggtttgca
    24421 agggagtact atggaaataa agatttaagg ctagaatact aagaatgaga gaaatggcga
    24481 tacccagttg ccataaaaca atagttcgac gtctcaatat catacttttc tccttaactg
    24541 tggattgatt atgctattta aaatatccgc aataagattg cccgcaaaaa caaataatgt
    24601 ccctatcatc acaatagcta gaagtagcgg tgtatcactt ttaagtcctg cttcagttag
    24661 ggtagaccct agtcctggat atgagaaaac ttgctcagca agaacggatc ctccaaacaa
    24721 ttctccaaaa taggaaaaat gcagtgtaat agctggtacg atagcatttc taagacaatg
    24781 atttttaaaa atttgccatt ccgtttcccc acgcgctctg gcaaataaga catattcact
    24841 agaaagtacc gacatcattt tagttctagt atgaagagtt acattggcaa tgcctagaat
    24901 acttagcgtg aaaacaggta aaataaggtg cttaatacga tcagctaacg taatatcttg
    24961 actcaaagta cctatcggag aagaaatacc aatcgggaac caccccagct ggacagaaaa
    25021 gattaataaa aaaatgagcc caatccaaaa agttggtact gatatctgaa ggtaagaaaa
    25081 ccaccttaca attcgatcaa gcaatttccc ttgatggaaa gctgataaag ttcctaagat
    25141 aaatccaata agacccgata agacccagga gagtcccata agtatgaaag aggcacctgc
    25201 ccgtgatcta ataatatcaa taacaggttg ccgataaact agtgaagtcc ctaaatgtcc
    25261 ctgtataaaa ttttttaacc aaataaaata ctggaccgga gctggcttat ccaagccata
    25321 gtggtgagca atcgctttgt actgaacagg ggttagtgac gtgtcataat tgacacttgc
    25381 catgactgga tcaaccggtg attgttttag taaaataaag gtcaaaattg atactccaaa
    25441 aacgagtgac aaacacctaa gtatcttcca gataactaca gtggtaatat gtttcacaga
    25501 taaatctcct taattgttaa aaaaggagtc gaggaaacca ttcacaccat aaggtgtaca
    25561 ttgttttctc tgactccaaa cttaaagtag cctacattaa ctaaattagt tacttagcag
    25621 attcatccca agtccattca gcaatgttag tcaataatga ccaatcatga ccatgactat
    25681 ggacaccttg tttacctaca ttgatacgtt tatcaccaat ataagtatgg ttaagactca
    25741 ccaaccatac atttggtaaa tctccaagag tggaagcacc tgttttgcca tcccactgag
    25801 ctaacttcca atatttgtta gctttatcaa ggtcgggaga tgtcattgct ttgtcaaggt
    25861 acttagtcac agtaggattg ttataaaaag taatattggt ccaaccttta ccagctaaac
    25921 ttgggtaatg tgattcataa aattgctgcg catgatgacg tcctcccgca taaagtaagg
    25981 ctgaatcatg tgacttagtt gccatttcat cccagttact agccttgagt ttaatagtaa
    26041 tacctaaggc tttagcttgc tccgctactt caacggctaa gtttgctcgt aattgatcat
    26101 tagtagggta gtaaaggtca aattccgctt taagatttcc ttttttgcgg ctaccgtctg
    26161 cttgttcttt ccatccagct tttgtcaaaa gttgcttagc tttagctact ttattatctt
    26221 taattgctgt ttttggattc cagaatggtg ttctatcaat aattgaataa gctggtttac
    26281 cataaccatt taaaacagta tccagaactt tttgtctatt taaaccaata gtcaaggctt
    26341 ttctgattgc tggatcacta gtgacatcat ttcctactgg ataaccgtct ggtgaatttt
    26401 tcacaacacc tttcttcaca taaggtagcg ataacccacg aacatcattt gaagcaatat
    26461 ctagaagacg tgttcctttg actttcttgc tagcaagctc tggcgttgcg taaatcatgt
    26521 caacatcacc agattctaaa gcagctagtg ctgtattttc atcaagtaat acccaagtcc
    26581 actttttaaa ataaggcttt ttaccgtgcc aatatggatt tcgaacgaag attgcttgtt
    26641 ctccagcctt atattctttt accatgtaag gtcctgaacc gataggattg ctcttatact
    26701 tatcattgta atgtttttta gggacgattg ggatttctgt caactgtgct gtaaatgtcg
    26761 aatgcgcctc agtcaaatga atatttacct ggtttttccc aactacttca acattcttaa
    26821 taaaggtcaa atcccaagct tttccatctg ctttcaacat atcataagta aacttaacat
    26881 cgtcagcagt aacaggctca ccattggaga atttaaaatc atcatttaag tcgaacgacc
    26941 acgttaagcc atccttagag attttatatt ttttagcaag ctctcctttt atatctagtt
    27001 caggagaacg tttcaatagg gtactatgag taatattacc ttcattatgg attccataac
    27061 ggtcctttgg atcgaattca tgaggaagct ttgcccccat agagacaacc aattcatctt
    27121 tgggtcgttg ttttcgtgtt cgctcctttg tttgtgaatt ttgattttga caagctacta
    27181 aaaagatact cgccagaaac aaagcaacaa aagagatgta cttcaaatat ttagtcacta
    27241 ttaaacctcc ttttttaatt gataaataat atttttgaca acttttttca ttataacaag
    27301 aaaaaagtag gagaacaatt acttaactgg ttaattagaa aatttgttat tttatcatta
    27361 ataacttaaa gttaggtaaa acacaattct agactctagc tcataaacca tgttaatcca
    27421 atgtatttta atagacatca taaaaaggct gaataaattc agccttaacc acgctattag
    27481 ttatagcaaa ccaatcaaat gactaaagat aggaataacc aaatcaacta ccaaaccgat
    27541 aaccaccacc gctatagctg ccatcgcacc ttgcagttct cccaattcca atgcaaaggc
    27601 tgctccgact gtatgaccag aggtccctag acctaatcct gcaccaatct tactatcatt
    27661 caaatggaaa aatgacacta attttttacc taatgcataa ataataactg catttaagat
    27721 acacgccatt gctgtcacag ctgtgttccc tccaattgct gctgttattg gaagagcaat
    27781 tgctgttgtt gctgcttgtg gcaacattga agcaattccg acttgactaa gcccaaccat
    27841 tttagaaata gcgacgataa gcataaggga aacaatcata ccgattacta aactgctgag
    27901 aatttctacc caatattttt taacaacgtc atttttctta tagagaggaa ctgcaaaagc
    27961 aattgttgct ggatttaaaa accaaaatat taaatcgcca cctggtttat aagcttgtgt
    28021 ataaaatgtc ttaacatcgg ttcctaaacc ttttgacata agccaaagaa tgacaatacc
    28081 actaaccatt gcaaagaaaa gaggctgcaa aaggaagaaa cctttactct tcttaaataa
    28141 atgttgtcct atcgtataga gtattaaaga aaagcaaata ccaaagatgg gtgtttttaa
    28201 gagttccata ttatttttcc tctactttct tactatagtg gttaggtaat atctttttaa
    28261 ataaatctgt gacactgagt tctctgatag caagtattaa tcgtgcaacg tatgctacca
    28321 ctactaacat aacaacagtt gagattgtaa taacagcaac tagttgcaag ccttcagctt
    28381 ttaggatatc aaggttcgct gctactgata ttcctgaggg gacaaacata aaaccaatca
    28441 tgcttatcat caaagcccca aaagagtcaa cccactctac cttgataatt ttagcagtta
    28501 gcaatacgta cattagcacc aaaccaataa ccgtcgttgg gattggtagt gatttcggta
    28561 ataacattga aatcatttgt gaaactaata gaatagctgc atatattgac atttgaataa
    28621 acattggagc ttcttttgtt ttggtttctt tactggtgtg tttcatagtc actacccctt
    28681 ccttttatta caatgtttta acttgtttta ttgatagcta tattgtactt caaaataaat
    28741 aaaacgtttt cattttcagc ctcagttacc aaaaaaagaa gccgaattgc aaattcaagc
    28801 ttctaaacaa caaagggaag ttcgcaaaag cacttttaca tggttaaatg ctcatttagg
    28861 tcttttaaat acgatcgacc tactggaaat ttctctccat tagacataat tagtaacaaa
    28921 gtatgattaa accaaggttg aatttcctca atcatctcta gattaatcac tgtgtttcga
    28981 tgaatctgga gaaaatatgg tgctacagcg cgtgacttaa accaattgag tgttttccga
    29041 atagtgtaag ttttctgaac tgtactaaca gttaattcgc catcttcaac actagctgca
    29101 acgatatctt gcattttaac aacaacactg cgatctgata atgtcaaggt taacaattca
    29161 gcactggctt ttttaggtat tgcttgctct acatctgaag cagtggtgag ttggctcaat
    29221 ttcttcactt ttgatagagc catatcaacg cgctgttgtt caaacggttt catgatataa
    29281 tcaacagcat tactttcaaa agctttcaca gcgtagttat cataagctgt cgcaaaaaca
    29341 acaagtgggg gatgtgcaag ttggctcaac tgattagcca aagtaaagcc attttcttca
    29401 ctaagcgaaa tatctaaaaa aattaaatca atttgctgtc taaataagat tttttcggct
    29461 tcactgatat cctcagcctg aaaaatctca gggttgtcaa cctcctggct atgttcaact
    29521 aaaaacgata attcttgtct ggcaaacatt tcatcatcaa gaattaaaat tttcatgctc
    29581 atcctccctt attctattag gtattcgata ccaaactttt gtaccattct tgtcgctcga
    29641 aaaatgaagg caacttacac taccatataa taaattcagc ctgttattta gattaactag
    29701 agcagtacct gtccccttac tctctgcaac tgtttcttga cctaatttat caatgatagt
    29761 atctgagatt ccttgtccat tgtcactaac agaaacacaa taataatgac catctggctt
    29821 tatttgaacc aatatatggt tgtccgtctt acgttctttg aaagcatgtc gaactgcatt
    29881 ctctaccagt acctgtaaac caaaaggcgg taacttcatt ttttctggtg cactaatatc
    29941 ataagataac tgatatttat cagggaaacg taatttttca acattcatat aagcatccac
    30001 atgtgatttt tcttgctcaa gcgttacctc acgatcctga ccaccttgca aacttgttct
    30061 aaaaaaagta cttaactgca tcagtgcata acgtgcttta tcagaatcaa tacggattaa
    30121 tgcactaatt gtgttaatgg cattaaagaa gaaatgaggg ttgatttgtg cttgtaaagc
    30181 ctttatctct gccatactgg ctaacttatt ttgttcctct gttatcccca ttgccagttg
    30241 tcctgaaaat atttgcgcta aaccaaggac taggttttcc tccacctcag acattgtctt
    30301 atctcctgca aagtacattt ttaaggcaca cacagtttta tcatttattt ttagaggaac
    30361 tacaatagca gaatttaact gacagttgtg atctggacaa gaaatcgccg ctttatcttg
    30421 cgcaattctt ggttcgccat caaaaataac acttttagat aagtctgttt tgaccggttg
    30481 tcctgcaata tggtgatcat ggccaacacc aatatgagct aatacgtttg accgatctgt
    30541 taatcccaca gcatcaaagt tagtatgcct ctttataatt tcgcaaacgc tcctagcaga
    30601 ttgcggtgtc aaaccttgtc taaggtaggg cagagtctgt cgagtcaatt caagaacatc
    30661 tctcgtttga actgcgcgta actgactttc atttgacaaa taagttttca aaatcgcaag
    30721 gaaaagtgtg gaacctaaac tatttaaaat catcattgga atgacaatca ttttgacaag
    30781 ttcccatcct gtaaaaatgc caacaaatag catctggata ctttcggcaa taatactaat
    30841 taaaataact tggcttgttg aagggtagag atggttttcc ttaagcttat caccaatctt
    30901 tccgctaaca atgccgacta gaactgaact gacaatatag aaagaacctg aaaagcttcc
    30961 ttgaaaaaag cgatgaactc ctccaataaa accaacaatt gatccaacca gaggtccacc
    31021 aaccaaactt gccgttgtaa taactaaagt ccttgtatta gcaagtgagt cagaatggga
    31081 aatcgttgtt agaaaagggc gctcgaccaa acttcgatcc ccttttattt caattcctgt
    31141 tatattagat ataataacaa acaagccgaa aatgatgaca aggactaccg tttcacgttt
    31201 agaccgctct tcaattaact gtctgaaata actattattt accaataaaa aggctaaaat
    31261 cataataatt cctagccttt ggaataacaa caccatcaac atatgcttat cctcctcaag
    31321 ataccatttt actccggaag ataaatatag atatcttgat aatctgaaat aaccttgtct
    31381 gccattgata aatcttgcgg tggataatca ggattggcaa agccaaagca atacattcct
    31441 gctgccttag cagctaaact cccatttctt gtatcttcta taactataca aactttaggg
    31501 tctacatcta aaagctctgc agctctaaga aaaacatctg gtgctggttt agaagacgat
    31561 acatcttctc ctgtaaccat gtattcaaag cattctgtca cacccaactc ttttagatta
    31621 cgcttaatat ctaccattgg agatgaggac gcaactgcta agcgatagcc atgttggtgt
    31681 aaccaatgaa tcagttgctg agctccttta atgggcctaa caccatcacg cgtcacaatc
    31741 gcttgtcgac gtctgttcat ctcagcaata tactctttta ctgttttcgg taagccaaac
    31801 tcctctttca tagcttgcca catgaattca aaagtagtcc ccatgtactg gtattgatac
    31861 gagacgtccg tatcaatacc ctcctcacgt aacatttctg tcttattatc taaaaacgtg
    31921 tactcggaat caacaataac gccgtccata tcaaaaataa tgactttttc catcttaaac
    31981 ctgctttcta tcccctttat tgtaatcgtt tgcctttcta ttttcaataa aaaaaggttg
    32041 ctttctgtgg aaagcaacca gtatatttag aaatgatatt ttgagagtga ttttatttaa
    32101 aaggggagct cctcttcttc caatactaaa tctgacaaat caccagaaac attattttca
    32161 cgcatagcac gttgggcacg gctttctaac aactgaaatg atgatgctaa tacttcagtg
    32221 atatagtgcg tttgaccatc cttttcgtac ttgcgcgtac gcaattcacc atctattgaa
    32281 attaaagagc cctttgtccc atagctcgca agggtttccg ctagacgacc ccacataacc
    32341 acattaataa aatctgcttc acgctcacca ttacttcctt taaagcgtct attaacagca
    32401 acagttgcac gcgttactga cttgtcagtt ggtgttttta ccatctcagg ctttgctgtt
    32461 agacgcccaa tcataataac tttattatac attgattacc tcctcactat tattattcga
    32521 aaataaggaa gaaaaatgct aaaatatatg aaattgttct gggagaatta agatgacaaa
    32581 tgtaactagt tataaagaaa tgctggctaa accttgggga aaaatacaat atgaaataac
    32641 ttttgctcaa ttaagccata tcaaaaatca gaacgtttta gattttggag ctggtttttg
    32701 tctcaccgca caacatctag caaaagaaaa caatgtaact gctattgaac ccaatcctaa
    32761 actgctttac gataatcaaa gtgacaatat ttataaaatc ctaggttcct acgaagcatt
    32821 aagagctttg cctgatcaaa gttttgatac tattatttgt cataatgttc ttgaatatat
    32881 agacaaacat aatcatcctg cttattttga cgaattttct cgacttctaa aacctaatgg
    32941 agaactatct ctcatcaaac ataatataat tggtaaaata ttacaatcag ttatttttag
    33001 caatgatacc tccactgcga tggaactctt aacaggagaa gcaaacttta aaagtgccag
    33061 ttttgaccag ggaaatattt ataccttaga agaactaaaa caaaatacaa atttattagt
    33121 tgaacgttat cagggtattc gtaccttcta ttcattacaa ccaaaccatt ttaaaactga
    33181 aactgattgg ctaaacaaaa tgcttgctat cgagctaagt gttgctgaca aagctcctta
    33241 taaagatatt gcttttttgc aacacatcac acttaaaaag tcattgtaac ttcttaagtt
    33301 tacaactaaa aatcaactta gctgaagcta agttgatttt tactaatttt tccaatgttt
    33361 attggcgtcg aaagactctc caacaattac ctgatcagat aattcaatga tacctctgac
    33421 ttgaggagca tttggaagag ctaactcgcg agcactacat aacattccaa agctatcttc
    33481 tccacgaagt tttcctggga aaataaggct accatttggc atcatagctc ctggaagagc
    33541 tgcaactgtt ttaaggccta ctgaagcatt aggtgcccca caaacgattt gaaccgtctt
    33601 cccatcgttg atttctactt gacagatgtg taaatggtca ctatctggat gatcaatcat
    33661 ttctttaatt tgcgccacaa caaatgtagg ttcaatatta ttaacaagag agtcttcgaa
    33721 tccttctttc agtagttctg cattaaggat tttaataact tgatcagata aagttacttg
    33781 acctactccc tcaatagcaa ttaaacttga cacttcaaag atattccaag caactgtttc
    33841 tttactatct tgcagataga cgcgtgctac ttgtccgcga cgatcaacat ctagcttagc
    33901 tccttgacta tccttaacaa taaccattaa ggtatcacca acatgctctc tattataagt
    33961 aaaaatcatt tattctcctt taaattttgc tattttttgt cttatttatg gttaatttca
    34021 gcgagaaatt tagtaatttc agcctttgtt ttgcgattct tattaaccaa acgacccagt
    34081 tcctgaccat tttccacaac aacgaaactc ggaatcccaa aaatattcca ctgctgagct
    34141 aattctatat agtcatcacg attaacacgt acaaagacaa aatctgagaa atctttttca
    34201 attgatggca ttactggata tataaattga caatctggac accaatctgc tgtaaaaaag
    34261 aaaacaactt tcttagtgct atcaatataa gctgctattt cttcataaga ttctggtaaa
    34321 atcatttgct cacctccaca taatcaagaa caccttgacg ataaatgaat tcgaaaattc
    34381 tgccatcttc caagacaaga ccaccactgg tcattttaat atcagattca aattcattaa
    34441 aataaaatac ctgaatatca cccatctgac taaaaagttc tcttaaggtc tgagtgattt
    34501 gctgacgttt ataatctgtt acttttttag ttagggtata accagcagct gccagtaaac
    34561 ccacaccagc aaggccagtc ccaaataaga tttttttatt tttcataact gttattatac
    34621 catacccccc ggaattagac acaggaattg atggaagaaa tccacataac agttatgaaa
    34681 aaaaacaact tattccttgt ggtataatag gatgacattg tcttaagata ccattatggt
    34741 taatcattca aacagaaaga aaaaacatga aagaaaaatt attagtacct gctttaaatt
    34801 gtttgctagg tctactcttc ctaggtgatc actggttttt atttcgtctt caaaatggag
    34861 aactttttgc tattgtgacg ctaatactgc tttgcacagc ttcattttta atcaataaga
    34921 atcaccccaa aaatttgact ttaattgcta tggttatcac ttttttagcc gtattatctt
    34981 tcctatctac ctttttatta gcttaagccg ataaacatta tctccgttgt attcaaccca
    35041 gttttcacta taacaggctt tgtcagtcat aacagtcttc tggtagtact tgatattctt
    35101 tttgaaagtc tttatattga ctgaaacctg ttctgttctt tatttttaat taaaaggtgc
    35161 taaaatagta tttgtagaat tgaggtaatt atgtcagatt tatttaacaa aattaaaacc
    35221 gtaactgagc ttgatgggat tgctggctat gaacacaata tccgcaactt ccttcgtcaa
    35281 gaaataactc ctttagttga tcaagttgag acagacggac ttggtggaat ttttggagta
    35341 aaaaatactc atgagactaa tgctcctaaa gtcatggttg ctgcccatat ggatgaagtc
    35401 ggctttatgg ttagtcatat tcagccagat ggaacatttc gtgtacttga ggttggagga
    35461 tggaatcccc tagtagtcag ctcacaacgc tttaccctct acacacgttc tggtgatgcc
    35521 attcctgtta tatcaggctc agttcctcct cactttcttc gtggacaaag cggtggaaca
    35581 acattaccca aaattagtga cattgttttt gatggaggat tcacagataa aaatgaagct
    35641 gaaagctttg gcattgctcc tggcgatatc attgttccta aatctgaaac cattttaact
    35701 gcaaatcaaa aacatattat gtcaaaagct tgggataatc gctatggtgt gcttatggtg
    35761 accgaattgc taaaaagctt aaaagatcaa agtcttagca acacacttat tgctggggct
    35821 aatgttcaag aagaagtcgg acttcgtggc gcacatgttt caacaactaa attcaaccca
    35881 gatatcttct tagctgtcga ttgttcccca gctggagata tttatgggga acaaggcaaa
    35941 ataggagagg gaaccttaat ccgtttttat gatcccggac atatcatgct taaagatatg
    36001 agagatttct tgcttacaac agctgaagaa gcaggtataa aataccaata ttatgctgca
    36061 aaaggtggta ccgatgctgg ggctgctcac ctaaaaaata gtggtattcc ttctacaact
    36121 atcggtgtct gtgcacgcta cattcattct catcaaacac tctacgctat ggatgatttt
    36181 ctacaagcac aagcttacct tcaggccatc gttaacaaat tagaccgctc gacggtggat
    36241 attattaaag gttattaaga tagaaaggat gggagtttca taaggtagac acattttagt
    36301 atctcctttc atccccatat caacattatg aaaattggaa ttatcggcgt tggaaaaatg
    36361 gccagtgcaa ttattcaagg cctcaaacaa acccaacatg acattgtcat atccggatct
    36421 tgcttagagc gttcaaaaga aattgcagaa agacttgatg ttacttacgc cgaatctcat
    36481 cagtcattaa tcaatcaagc tgatattatc atgttaggta ttaaacctca actatttgaa
    36541 aaggttcttc ttccacttga tatcacaaaa ccaatcatct caatggcagc aggaatctcc
    36601 ttagcgcgtt taagccaatt aactcgttct gatttaccat tgattcgtat tatgccaaat
    36661 attaacgcac aaatacttca aagttgcaca gctatttgtt acaacaatca tgtatcagat
    36721 gagttacgac aattagccaa agaaattacg gatagttttg gaagtagctt tgatatagca
    36781 gaaatcaatt ttgatacctt cactgctcta gcaggttcta gtcctgccta tatctatcta
    36841 tttatagaag ccttagctaa agcaggtgta aaatatggat ttccaaaaga acaagcactc
    36901 tctattgtag gacagactgt cttagcttct agtcaaaacc tattacaagg acaaaatagc
    36961 acctctgact taatcgacaa catttgtagc ccaggcggaa ctactattgc tggcctttta
    37021 gacttagaaa aaaatggtct gacgcatagt gttatttctg ccattgatgc tactattgag
    37081 aaagcaaaaa aactttgagc aaattgctca aagttttttt agttattcat cattgtactg
    37141 attaacacgc tataagcaag agcattgtta ataaaatgaa tcattatact atattctaag
    37201 tgctcagttt tataataaac aaaagataaa atagcaccca tcccagcata aataataaaa
    37261 ctacctatat cagaagggga atgaataaga gcaaaagcta aagtaccgat gacaaaaccc
    37321 catttttgat gtttcgcaaa aagttcttta gggataatat agcgaaatat aatttcttcc
    37381 ataattggtg cccctattat ggcaaaaatt aacatcaaag ctagtggaag ttgctttgtt
    37441 aattccataa ttactgtttg atttgctgta tctttaaccc cttgcatagt cataatgagg
    37501 taaccaataa tttgtggaac cctcaatgat aaccaaccta agagaatagt aactaatgct
    37561 ttccttgtca ataatacttt ccatctgatt atttgtttaa ccttggcata ataaaggaaa
    37621 agagcaataa taagtaattc taagccaatt aacaatatac tagtaaaagg cgtttgatag
    37681 tgattcttta ggagccacac agccaatata ggaacaagtt gattaataac taaaaatgct
    37741 attagcaaag ctaaaatttt gcctttttta ataataaatg acacaaaaac ctccaaaata
    37801 aattattaac ttttttctat cataacatat tttcaaatct tatagattta tctcacaata
    37861 acttctgtaa aataaagtaa attaataaat aaccgtataa aacaaaagag agtatcaata
    37921 gcaagaactt tgcataggaa aaagttatac caaaccagac attaatcaga cacaatacca
    37981 ctaaaacaag gacagataaa aggaaaagga gtcgacaaga catattagca ataaattgat
    38041 tacgctcatc aaaaactttg gtatagtaat gttgaaatcg cttttcacta aatgttatca
    38101 ccaaaaaata aaaacttcct gcaaataagg caatacctga accaaaggaa aaacctttag
    38161 taaaattaga ttcaaaggta tgtagaaatg atgatactat cataatcaaa ctacatacac
    38221 taaaaacaaa agacaaaaat ctcatatata ctttgtaatc cttcggagat actttactat
    38281 tgggtgacaa taatcctttt attaacatag aatactactt tctaaaactt caatttaatc
    38341 atcagctatt atatcgtttt cttcaatgaa gcatagcgat aaatagcaaa aatagacatc
    38401 gcaatgctaa tactaatagt actgataaaa acatattgct ggtaaacgat tggtggacaa
    38461 aaatcaatag tccacatcct tgacatatta cagattaact tttaaccttt tgtcttttgt
    38521 ggactgtatt tttccattaa aattctatcc tccttcactt tctgtataaa taaaaacttc
    38581 ctcaatttgt ttatcaaaat aacgcgctat cttaaatgct agctccaaag atgccgtgta
    38641 tttctctttt tctagagaaa taatagtttg cctagtaact ccaagtgcaa ctgcaagttc
    38701 tgcttgactt aatttcctag attttcgtaa cttttgtaga gaatttttca tatcttacac
    38761 ctttttgtat agttagcttt acatttatag tatagttaac tatacatttt ttgtcaagta
    38821 cattttacat acctgttcaa aaaacaaaaa agttgatagt taaactatca acttttctgg
    38881 tcttatcaat tttttgataa cttatttagc tttaaggcgc tcaacgtcac gcgcaatcat
    38941 taattcttca tcagtaggaa taactaaaac cttcactttt gaatctggtt ttgtgatatc
    39001 tccaaaataa ccaaaaacat ttttttctgg atcaagttca atgccaaacc atgataatcc
    39061 tgcgataaca tcttgacgca ttaatggcgc attttctccc atacctgcag taaaaataat
    39121 agcatccgcg ccatttaata ctgccaagta ttgtccaata aatttcttaa tacggtcaat
    39181 aaagacatta taggctaaca ctgcatttgg atctttactt tgaagacctg cttcaatatc
    39241 tcgcatatca cttgaagtgc ctgatactcc aagcaatcct gattgtttgt taagcatatt
    39301 aacaacagca gctgcatctt ccaattctgg atcattagct acaagataag ggataatcgc
    39361 aggatcaatg tctcctgaac gtgttcccat cattggtcct gcaagtggtg tgaatcccat
    39421 cgatgtatca atagactgtc catgataatt tgctgtaata gaaacaccat tacccacatg
    39481 tgctgtgatt aattttaatt cttctaaagg acgtcctaat tgcttagctg cttcttgtgc
    39541 aacgtattgg tggcttgtcc cgtgtgcacc gtatttacgt actttataat cagtatagta
    39601 cttttgtggg attggatata agtaggtatg aggttgcatt gttgtatgga aggctgtatc
    39661 aaatacacat acactcgtga tatctggtaa gatttctcta aacgcacgaa tccctgctgc
    39721 agctgctggg ttgtggagtg gagctaaagc tgataattct tcaacctgtt ctacaacttt
    39781 atcatctact aaagcagact ctttaaaata ttcaccacca gcaacgacac gatgaccaac
    39841 acctgtaatt tcattaaagt ctttgataat accatgtttt gttagatctt ctaaaagaat
    39901 tttaacagct tgggtatgat ctacgatatc taaaatttgt tcatcttttt tatcgtcaaa
    39961 ttttacagtc gaaatagaat ctttcaaacc aatacgttcg ataatccctt tggcaactac
    40021 tttctcctct ggcatttcat ataattgcca tttcaaactt gaacttccag cattaattgc
    40081 tattgttttt gacataattt acctctctaa agcgttttca caatctctat tctatcatat
    40141 tttttgagta atgactattt tctctgctcc atttttgaaa attttcaatg aagtttgcca
    40201 tattctcacg attttgcaaa tctgtcagcg gatatacaaa ggtttctggt ttttgttctg
    40261 cttgcttctt gagaacaaat atagatttcg cattttgacg acttccaaaa atagtttctg
    40321 gtagagttaa aacggcaatg acatctgcat atccttttaa ccactccttc agcaaatcac
    40381 tttgtggact tgttaaaagg ttttcgggtg ctaaaaatat agcgattcca tcttttttca
    40441 aatatttaag agattgctcc atcaatagat ggtgagcata ggtgtgctct ttagaacttg
    40501 atacagcata tcgtttagca atatcatcat taggataata gccaactggt aaatcactaa
    40561 tgattacatc gctttctttc aaaatttgtg gtctaacggc atcctcttgg ataaattggg
    40621 cactagaacc tataatttca gcaatgcttg ctgatagatc aatcaaaaga tcatcaactt
    40681 caatgcccat ataatttaac tctttcgagc tgttattgag gagagtctga gctaaattcc
    40741 cagttccact tccaatttcc aagacatcca ctgtctcttg actcgttaat tcttccaaaa
    40801 gaaataacaa gataaaacca atactatctg gcgtaaactg atgattagct tgtaattgct
    40861 ctgtttgcgc agatttgata aaaatgaact ggaaagtgcg acgccattct tcttgactta
    40921 agtcaagttg acgtaatttt tggttattca ccacaacaat atctaaatca caacttgaac
    40981 caaggtaata agagttctgt tcaattaagg catcataaat atgagttttt aattggttct
    41041 caatcgtttg gatattttct aaaatcagct cataggctgt ctcaattttt tcaaaattca
    41101 tgatttcctc cagaatttat cataacaaaa aagacagcat ctgaaaagag aagcaaacaa
    41161 aaatcctgtt tttaagcaaa accttgtctt actaatattt catcactgta tatttttaat
    41221 gtttatggct tgctttatta tcattagact tgagaggtac ttggagagtg tctattttac
    41281 gataaaactt atggttatag tttcctccct tatccaatgt aaccttaact tgtaaatagt
    41341 tcccttcttg gaggtatgaa gcatatcctc ctgtaaaata acattttcct gatatggaat
    41401 cgccttctat tgttttataa gctaactgcg ctgttagata agctttactt gcttcaattt
    41461 ggctatgata ctgtctttct gttgccaata ttcttgctag ataaaactgt aagacgagtg
    41521 taaatacagc agccaataca atagcttgca aaaggatgcc agctttaagt ttcttcttta
    41581 aaatcatagt aaaatgtcct ttttaacccg tccttaaaat aaaaaacaag ttttaccata
    41641 cttttggtct gactcatttg acaattgtct aacccataaa ccattggttg ataacctcga
    41701 ccattataac ctgtcttacg gaaatcatct ttattagatt tgccaaaggt tacaatctta
    41761 tcttgcttac gtaaataaag tttgttctgt cttaaatatt ccaagtgagc gccttcgaat
    41821 tctgcattta gttgctgagt taataacacc cattcagact gactggaaga agacatcact
    41881 actatctgtt gagccaacaa ttttgttagt ccttgataaa ctagcaaagc tcctgtgatt
    41941 gtaaccaatg ctaccaaaca ttctaaaatg tgtaaatgct ttaaccttct tatccttaca
    42001 ttttaacaat aaatttttca tgataatatc tccatatttt gatacattgg taacagcatt
    42061 gctgcataaa tcataacaat gataagagct acaaaaataa aaataacggg ttggattaat
    42121 tgggtcgctc tagctaattt tgtaaaaaaa tcctcccact tctcatcagc atatatatct
    42181 aactctgttc ctaatttcgc cttaacttga ccatattcaa tcattaaact aagctcagtt
    42241 aagaaaaacg gatagtctaa tactttttgg tgaaatgctt tacctgatag aaaaccttct
    42301 tccatgtcat atcctatttc cctaaaaagt ttggatttct gattttgcat tactttaaca
    42361 atttggtcca attcaacacc ttgacttaat aaatttcccc actcacgggc atagtaagca
    42421 gttaaataaa gcttaacata tgatccaact aagggaattg ttgttaaaaa acaagctact
    42481 ttaatgcgcg acaatcgctt ttgaataata taaaatatta aactaaaaat aagtacaact
    42541 gctaaaagta ataagaaaat attcggcaca tttgtaatca gtctagttgc aaaattattt
    42601 tctcctaatt ggggcattaa ataatgccta aggccaatca taattagcac cagaaaagac
    42661 aataatatca atgggtaagt agctacttct attacttttt ttctaactaa caaaagatta
    42721 gctaaataag actcaatctt tagtaggctc cttgaaatgt taccatgcaa atcagctaat
    42781 gcaacctgtg tgataacagt gtcagaaaaa cctaactctc ctaacatttt tgataaatct
    42841 tttccctcta ataatgcctt attcatccta tctgtataac aatcagacaa taacttactc
    42901 ctctttaaga aggtgaccat atcagttaaa gaaaaaccac tagcaaaaag attattaaaa
    42961 agttggacta ctttccgttg tttcttgagg gataattttt tcgacttgtg cctgtttctt
    43021 actgatatgt ccttcttcag ccaagatatc cacttgtcta ttccacttgt ctgatgagtg
    43081 ttttttaaag ttacttgtct caaagtcaat taggcttcct cctccaatta aacgttgata
    43141 tgctattaat tttagactat tttctaactc ttgatagtta acccctaatt ctataagcct
    43201 atcatagact ccgggaatac ttttagcatg aatagtagaa aaaaccatta ctcccgttaa
    43261 acttgcacga ataacagcac gggccgtcgc ttgatctcta atctctccga taattaaaat
    43321 atctggacga tgccgtaaag acagtttgat taaagcatca taagtcattc caatatcctc
    43381 attcaattgg agttgtaaca tcttgtcatt cttgatttct accggatctt caatcgtgat
    43441 aatttgctta tttttaaata cttctgaagc taattgatac atgagagttg ttttaccact
    43501 ccccacaggg ccggaaaaaa gatatagccc tcttgcacac agtacttcct tcatttgctt
    43561 tatattatca aaccaatatt ttaagtcctg atgacctgaa tacaaagtac gaataactaa
    43621 agattcttga ccacgataat ctcccacact tgatagtcgt aatgaaacca gtcttccctc
    43681 tgacagttca tagtcacaag aacctaattg acttcgtctt ttttctccaa cgttcatgcc
    43741 tgccacaaat ttaaagtgac taataagact agccatccta ttaaactcaa aaacatcaat
    43801 aaaccgcctt tcatcatcaa tacgcatata gagttcataa caatcacctt tgggaatgat
    43861 ataaatatct tgagcattta cttctactgc ctgatgaatg acgtgctttg ctaatgattg
    43921 aaccatcccg tacctcctta accatatatt cgaaaataaa ataaaaaaat ctcaccaatt
    43981 ggtgagattt tttcaatgat ttcttgaggg gtagtaacat aaatattatg aagcagaggc
    44041 tgaacaaacc tattatatcc ccataaaaac tatctaactt ttttaaacta cttttaatta
    44101 caaatttaaa gaacatgccg atggcaactc agctatctta ttaaatactt caaatcgctc
    44161 aatagattct tccttaggaa tttcttgcca ttctttcaat aacattaagg aatgatactg
    44221 ttggaggtac tcatcacatt cttcacacca acgtttttct tttatactcc aaaacgttgc
    44281 caacaaacta gacttacttt gaaaataagg ccacttttcc tgtcccctat cccactcctc
    44341 ttgaaaatag agcaaagctt ctgataaggt atcatactca gcaatttcag taatatcttc
    44401 ctcccaacct tcaataaacc accatggttc ccaatcacca aacattttta caacttgata
    44461 cataatacac ttcctttaga gagtattata taaaaaataa gaataaaata aaaaatatct
    44521 gctcattcca aattcttgta aaaaaagaac ctgagttaaa ctcaagttct ttttgattaa
    44581 tcttctgttg taggtgttga tacttccgta acttctgcat caacaggagt tccttcaata
    44641 atctcaactt cattaacagc aagtggttca atattacggt aacgagccat accagtaccc
    44701 gctggaatga ttttaccgat aataacattt tctttaagac caagtaagtg atctttctta
    44761 ccacgaatag cagcatctgt aagaacacgt gttgtttctt ggaaggatgc agctgataag
    44821 aatgaattag tttcaaggga agccttagta atacccataa ggacaggacg gcttgtcgca
    44881 gggattccac cagaaataac aatatctttg ttagcatctg taaagtctga aatatccata
    44941 agtgtacctg gaagaagatc tgtatctcct ggatccatga cacgaacttt acggagcatt
    45001 tgacgaacca ttacctcaac gtgtttatct ccgatttcta ccccttggct acggtaaact
    45061 ttttgtactt cagcaagtag gtacgtttca acagataaag tatcacgaac ttcaagaaga
    45121 cgttttggtt ggatagatcc ttcagttaag gccgcaccac gagctacttc atcaccaact
    45181 tcaactttca tacgtgctgt aaatggaaca acgtattctc cctcacctgt ctgaccttta
    45241 acgaatactt tcttagtacg tgttgaagag tcttcttcaa ttgcaacgac ttcaccctta
    45301 acttcagtaa tgactgcctc acctttaggg ttacgtgctt caaagatttc ttggatacgt
    45361 ggaagacctt gtgtaatatc agtatttgaa gcaacaccac cggtatggaa ggtacgcatt
    45421 gtaagctgtg tccctggttc accgatagat tgagctgcaa tagtaccaac tgcttcacca
    45481 acctcaacag catcacctgt tgccaagtta ataccgtaac agtgacgaca aacaccatgg
    45541 cgagtgttac atgtaaatac tgatcggata gttacttcct caacgcccgc tttaacaact
    45601 ttagcagcca tatcttcagt aattaaggta tccgcaccaa cgagaatttc acctgtctca
    45661 ggatgtttga ttgacttctt agtataacgt cctatcaaac gctcttcaag tgtctcagta
    45721 acctctttac catctgtaat tgctgtgatt gtcaaaccgc ggtctgttcc acagtcatct
    45781 tcacgaataa taacatcttg ggcaacgtca accaaacgac gagtaaggta acctgagtcg
    45841 gctgtcttaa gggccgtatc agtcatacct ttacgcgcac cgtgagttga gaagaacatt
    45901 tccaaaacac taagaccttc acggaagttt gataagattg gcaattccat gatacgtccg
    45961 ttaggagcag ccatcaaacc acgcataccg gcaagttgtg agaagtttga gatgttacca
    46021 cgagctcctg agtccatcat cataacgata gggttctttg gatcttgtgt ctcaataaga
    46081 cgtttttcaa gtgcttcttt agcttcacgc catgttgttg taacagcaac ataacgatct
    46141 tcttcagtca tcaaacctcg acggaaggct ttattgatat cttcaactct gtggtgagct
    46201 gcatcaataa tctcagcttt attgtcaata actgggatat cagcaatacc cacagttaaa
    46261 ccagcaagtg ttgaatgata atatccaagg tccttcaaac ggtcaaggaa agcagaagtt
    46321 tctgttgtac ggaaacgttt gaaagtttct gcgataatat taccaagatt tttcttctta
    46381 aagggaatat taatctctaa gttatcaata actgcttgaa tatcctgacc tggctctaag
    46441 aagtatttat ctggagtttt ttctgtcaag tttgcattat taggttcaat taagtacggt
    46501 aaatcttcag gcatgatatc gttgaagaga atcttaccaa ctgttgtaac catgatttta
    46561 tgtttttgct cttctgtcca aggcttattc ggcattgaat caacagcgat accaacacgt
    46621 gtatgtaaat gaacataacc attttgatat gccataactg cttcgtcatg atctttgaaa
    46681 atcatgcctt caccttcacg accagcatct tccatagtca ggtagtaatt tcctaaaacc
    46741 atgtcctgag atggtgttac gactggctta ccatctttag ggttaaggat gtgctctgca
    46801 gcaagcatca aaagacgagc ttctgcttgg gcttcttcag acaatggaac gtggatggcc
    46861 atttggtctc catcaaagtc agcattgtag gcctcacata caagtggatg aagacgaagc
    46921 gccttaccat caataaggac aggttcaaac gcttggatac ctaatctgtg aagagtaggt
    46981 gcacggttta gaagtactgg gtgctcttta ataacttctt ctaagatgtc ccaaatacgc
    47041 tcgtccccac gttcaaccat acgtttagct gctttaacgt tccctgcgag gtcacgagca
    47101 acgatttcac gcattacaaa aggtttgaag agctcaatag ccatttcacg tggtacacca
    47161 cattggtaca ttttaagtgt tggaccaaca gcaataacgg aacgtccaga gaagtcaaca
    47221 cgtttaccaa gcaagttttg gcggaaacga ccttgtttac ctttaagcat atggcttaat
    47281 gatttcaaag gacgactacc tggtccggta attggacgac cacgacgacc attgtcgata
    47341 agcgcatcaa cagcttcttg taacatacgt ttttcatttt gaacgatgat gccaggagca
    47401 tttaattcca acaaacgtgc caaacgattg ttacggttaa taacacggcg gtaaaggtca
    47461 ttcaagtctg atgccgcaaa acgaccacca tctaattgaa ccatcggacg aagatccggt
    47521 gggataaccg gtaaaatgtt aagaaccatc cattctggtt tatttccaga ttttttaaag
    47581 gcatctaaca catccagacg acgaactgct ttcacacgtt tttgacctgt cgctgatttc
    47641 aactcctctt taagaactgc gatttctgca tctaaatcaa cgcgtttaag aagatcttga
    47701 atggcttcag cacccatttt agcaacaaac gaaccgtaac cgtactcttg aagtttttca
    47761 cggtattcac gctcagtaag gagtgacttc ggttctaatg gtgtgtccat tggatcgata
    47821 acaacataag ccgcaaaata aataacttct tcaagtgcac gaggactcat atccaatgtg
    47881 agtcccatac gcgatgggat acctttgaaa taccagatat gagatactgg tgcttttaac
    47941 tcgatatgtc ccatacgttc gcgacgaact tttgcacgag ttacttcaac gccacaacgg
    48001 tcacaaatga ttcctttgta acgaatacgt ttgtattttc cacacgcaca ttcccaatct
    48061 tttgttggac caaaaataac ttcatcaaaa agtccttcac gttctggttt caatgtgcgg
    48121 tagttgattg tttcaggttt tttaacttca ccataagacc atgaacggac cttacttggt
    48181 gatgctaatg tgatttgcat acttttaaaa cgatttacgt caaccactag ttttaccttt
    48241 cttcgattta tcatcatact agcccagcta ggatttcaat aaatcctagg actagcattg
    48301 catatcaatt ctatattcgc ttctagttat aaactagata aatttaatct tcttgaacga
    48361 cttcagatac ttgttcagct tgtttttctt cagcttcttg tttcacacgc gctttttcaa
    48421 ggtcatcaac gtgcatcaca tcatcatctt caccttcatc aagatcacgg agttcaactt
    48481 cgttgtcatc ttcatcaagg acacgcatat caagaccaag tgattgcaat tcttttacaa
    48541 gaacacggaa tgattctgga acacctggtt ttggaattgg cttacctttt gtgatagctt
    48601 cataagcttt aagacgtcct gtaacatcat cagatttgta tgttaaaatt tcttgaagaa
    48661 catttgaagc tccataagct tcaagcgccc aaacctccat ctcaccaaaa cgttgtccac
    48721 caaattgagc tttacctcca agtggttgtt gagtaacaag tgagtatggt cctactgaac
    48781 gggcatgaag tttatcatca accatgtggt gaagtttgat catgtacatg acaccaaccg
    48841 acacacggtt atcgaatggt tcaccagtac gaccatcata aagaacagtc ttagcatctg
    48901 aatccatacc tgcttcttga acagtttccc aaaggtcttc tgaagaagcc ccatcaaaga
    48961 ctggtgttgc aatatgaata cctaaattac gagcagccat acctaaatga agctccataa
    49021 cttgtccgat attcatacgt gatggcaccc caagtgggtt caacatgata tcaactggcg
    49081 ttccatctgg taaataaggc atatcttcaa caggaacaat acgtgagacg acacctttgt
    49141 ttccgtgacg accggccatc ttatcaccga ctttgatttt acgtttttga gcgatataaa
    49201 cgcggacaag catattaaca cctgattgca attcatcacc atttgcacga gtaaagattt
    49261 taacgtcacg aactactcca tcgccaccgt gaggtacacg tagtgaagta tcacgaactt
    49321 cacgtgattt atcaccaaaa atggcatgca agagacgttc ttcagcagat aagtcctttt
    49381 cacccttagg cgttacctta ccaacaagaa tgtcaccttc ttttacctca gcaccaatgc
    49441 ggataattcc catttcatcg agatcacgta gtgaatcttc accaacattt ggaatttcac
    49501 gagtaatttc ttcaggccca agctttgtat cacgcgtttc agattcgaat tcttccaagt
    49561 gaacagatgt atagacgtct tctttcacaa ggcgctcact catgataacg gcatcctcga
    49621 agttataacc ttcccaagtc atataagcaa caactgggtt ttgtccaaga gccatttcac
    49681 cattttccat agatggtccg tcggcgataa aatcaccttt ttcaacgaga tcaccaactt
    49741 taactaaggt acgttggtta taagcagtac ctgagtttga acggcggaat ttttgaacat
    49801 gataaacatc aagagaacca tcttcgcgac gcacttcaac tttttcagca tctgaaaaaa
    49861 taacacgacc gtcatgttta gcaatcacag ctgcacctga atcgtgggct gcttgatact
    49921 ccataccagt accaacatat ggtgcttttg gatcaatcaa tggcacagcc tgacgttgca
    49981 tgttggcacc catcaaggca cggttagagt catcattttc caagaaagga atacatgctg
    50041 ttgcgacagc aactacttgc ttaggtgaca cgtcaacaaa gtcaacaatg cttgaaggaa
    50101 actcttggtt attaccttga tgacgaccca taacgatttc ttctgcaaat gtaccgtctt
    50161 cgttaagctt agagttggct tgtgcaactg taaattcatc ttcttcatca gcagtcaacc
    50221 aaacaatttc gttcgttacc gcaccagtag aacgatctac tttacgatat ggtgtttgaa
    50281 taaatccata tttattgagg tgtccaaatg aagataagtt attaatcaaa ccaatgtttg
    50341 gaccttcagg tgtttcaatt ggacacatgc gcccatagtg ggtatagtgc acgtcacgaa
    50401 cttcatatcc agcacggtca cgtgtcaaac caccaggtcc taaggcagag agacggcgtt
    50461 tgtgcgacaa ttctgataga gggttgtgtt ggtccatgaa ttgtgacagc tgtgatgaac
    50521 caaagaattc tttaacagcg gcagtcactg gacgaatgtt gatgatttgt tgaggtgtca
    50581 agacttcatt gtcttgtaca gacatacgtt cgcggacatt gcgttccata cgagcaagtc
    50641 cgatacggaa ttggttcgca agtaattcac caactgcacg aatacgacgg ttacctaaat
    50701 ggtcaatgtc atctacctta ccgataccct ctgcaaggtt taagaagtat gacatctctg
    50761 ctaaaatatc tgcaggagtt aaggcacgaa ctttatcttc aggatttgag tttccaacaa
    50821 tagtaacaac acggtctgga tcagttggag caacaacttt gaatttttga agaataactg
    50881 gttcagtaac tacagcataa tcatttggtg tgtagacaaa cttgtttaag tctccatcga
    50941 tatgctctgc aattgaatca atgacatctc gtgtcataac ggttccagct tcaacaagaa
    51001 tctctcctgt ttcaccatca actaaatttt cagcgattgt ttggttcaat aggcgtgttt
    51061 taagattcag ttttttatta atcttataac gaccaacagc cgctaagtca taacgacgtg
    51121 gatcaaagaa acgtgcaact aaaagactac gtgagctgtc tgcagttttt ggttcacctg
    51181 gacgaagacg ttcatagatt tctttaagtg cttcatctgt acgagaatca cttggatttt
    51241 tatgaatgtc tttttcaata gtattgcgaa caagttcact gtcaccaaag atgtcaacaa
    51301 tttcatcgtc tccagagaaa ccaagtgcac ggactagtgt tgtaaatgga attttacgtg
    51361 tgcggtcgat acgtgtataa gcaatatctt ttgcgtctgt ctcaagttcc aaccaagctc
    51421 cacggttagg gataacagtt gacccataac caacttttcc atttttatct accttatcat
    51481 taaagtaaac accaggagaa cggactaatt gtgaaacaat gatacgttca ccaccattga
    51541 tgatgaaagt acccatttca gtcataattg gaaaatcacc aaagaaaact tcttgagttt
    51601 taatttcacc agtttcttta ttaactaaac ggaaagtaac aaaaataggt gctgagtagc
    51661 tagcatcatg gatacgagct tcttccaaag tatatttagg ttcttttaat tcataaccaa
    51721 caaattcaag atccatagta tctgtaaagt ttgaaattgg aagtacatcc tcaaatactt
    51781 ctttcaagcc agcatcaaga aaatcttgga atgaatcagt ttgaatttca attaagtttg
    51841 gtaaatcaag aacttcctta attcttgaaa agctacgacg tgtacggtgt tttccgtact
    51901 gaacttcatg tcctgccaag tttttagctc ctttttaaag atagagtgac taagttttta
    51961 tctcataaaa cttagtgagt ttatttcatt tgctcgccta acaagaacaa gcttatagat
    52021 gtcgtttgtg gtaattttca gaaagtttaa atatatatta caataacctt ttttcttaaa
    52081 attaggcaca aaaagagcag ctaaatctca ctttatcaag ttgatttaac tgctgtatat
    52141 ctaggtcgga caatatttca acctagacag acgacaaata gttacgtaca ttataactta
    52201 ttttcaccaa taaatcaagt tttattctct tgacttcaag gaagcttcta gaatttcaat
    52261 ttgttcatca acacccttga gttttagcat aacacgatga tttttaaaca tagcatactg
    52321 gtgtttttct aattctttga aagaagacta tacaagaatt aaatcatatt atcttggaaa
    52381 aaataaaagg agaacagatc actctattct cctcaaataa aactatcagt atcgtaaacc
    52441 tgttatcgtt ttccacccaa agtagaccaa gctttttgat aatcactatc agttccccca
    52501 atagcaaaac gataggtcat cgttccaggt ccattttttg cccaataact tgttgtgctt
    52561 tcacctccaa cagtgattcg acgaccgttt actgttacta cacctggctg taaacctgta
    52621 gacttaagaa ctttagcttt tataacgcta tcatcaagtc taaacctttc tgacttacca
    52681 aaagtattgc catctgcatt attaatagca ttaaccaagt gagccatata attagcatta
    52741 ttattgtaac ctgtcaattt cgctaaagag gcattattat catgtcctgc ccatccacct
    52801 aaagtcactt taggtgtaga aagcatcaac caaacatcag aagttgagtt agtagtaccc
    52861 gtttttccaa tccaatctac cccagctaaa ccactattta aaccttgtaa gcgattttta
    52921 aaggttgtag tctttccgga gttaattggt ccatgtaata gctgctgtaa gatagtagct
    52981 gttgctttcg aaaagacacg gacaggttta ctttcgtgat tataaatcac tttcccatta
    53041 ctatcctcta tactttctat catatactgc ttatgataaa caccaccatt tgcaatcatt
    53101 tgataaaggt ttgtttgctg agcaactgac gtatcaatac caccaccaag tggtaaactt
    53161 tctataccaa agttttcaat tggatagtct aatttctcca tatagttttt tacatcaacg
    53221 cctcgatctc gtagcatctt gtaagtccag aaagctggaa tgttccaaga aatgtccaat
    53281 gactcttgaa ggttaaccat agcagtacct tcttcatccg catgcataat cttttcgcca
    53341 cttgaatatg tcgttggata atttgaaaga acactaccac ttcctaacat cccttgatca
    53401 atagcaattc cgtaaggtaa tatcggttta atacttgacc ctggagagcg tgccgtatca
    53461 aaagcatggt tattttgatt ctcactatag ttacgaccac caataaatcc tataatagca
    53521 ccactagaat tgtccgttag tacatttccc atttgaacct tgccagtacc atcatctaac
    53581 aaccctccgt attgagctgc agcatcctgc atggcttgat aaacagattt attgatagta
    53641 gttttaatag tataccctcc ctgttgaatc tcttcaatag cgcggtgtct gtacgtcgct
    53701 ctagtctcat cattttttaa atcatgctct gaaacattat cctttttaat gaggtaattg
    53761 tacatcactt tttgagcttc agataatgcc gagtagtaaa gataatcatg atgatttgtt
    53821 gtcgccactg caggtttgat aaaatctttt ttgatgtcat aatctttgta ggatttgtac
    53881 tcatctttgg ttaaagctct cgttctatac atattgtata acacattttt ttggcgcttt
    53941 ataccaaaac taagatcttt atctgatttc aactgggcat ctgcagtata aggagaatat
    54001 acaataggac tttgcggtaa acctgcaagg aaagctgcct gaggaattgt taaatctttt
    54061 gcggaaactc caaaaatccc ttgtgctgct tcttcaatac ctgcaatatt ctgtcctttg
    54121 ttattgcgac caaacggtga tacatttaaa taatctgata aaatcgaatc cttatccatg
    54181 taacgctcta atgctagagc ataaataatt tcttttgatt tgcgtttaaa tgacggatca
    54241 tctcccaaaa tttgctgctt taataattgt tgtgtcagcg ttgaaccacc gctactctct
    54301 ccaaacccca gaacagatcc cgctgcagca cgtagtacgg ctttgggaac tacaccttta
    54361 tggtcattga aattttcgtc ctctgttgca atgatagcct ttttaatatt atcagatata
    54421 gcatcctttg ccactggtgt acgttgtaaa tcagtggcaa tctctgaaat ttgtgattta
    54481 tcagaataag ttaacctaga caccctagta agtgtattaa cttgtgtcac taaactattt
    54541 ttacttggta ctttaacaga gtcaacttga ctagcaagat accctactgc aagtcctact
    54601 ccgagcataa caaatagtaa aataataaca tagatgaaat ctgataaaag ctttacaatt
    54661 cttaaaaaaa tagaaccaaa ttcaagtggt gtgtaatcac ctaatttaga actattcaac
    54721 ttcttattac ctttaaacat gaataatacc tcatcctaca ttatatcaga tttaatataa
    54781 cttgcatatc ctaaagagtt ttgatatagt tgacattaat acacaacaag ttaataatta
    54841 ggagaaaaag atgaatattt ttgatgaact caaagaacgt ggactcgttt ttcagacaac
    54901 tgacgaagat gctttacgca aagctcttga agaaggttct gtctcttact atactggcta
    54961 cgatcctacg gctgacagcc ttcatcttgg acatttagtt gctattttaa cctctcgtcg
    55021 cttacaactt gctggtcaca agccttatgc acttgtggga ggcgcaactg gacttattgg
    55081 ggacccttca tttaaagatg ttgagcgcag tttacaaaca aagaaaactg tagttagttg
    55141 gggtaataaa atccgcggtc aattatctaa ttttctagag tttgaaacag gtgataacaa
    55201 agctgtattg gtaaataatt atgattggtt cagcaatatt agtttcattg atttcttacg
    55261 agatgttggt aaatatttta ctgtcaatta tatgatgagt aaagaatctg ttaaaaaacg
    55321 tatcgaaaca gggatttcct atactgaatt tgcttatcaa attatgcaag gatatgactt
    55381 ttacgaatta aataagaact ataatgtcac tttgcaaatt ggtggttctg accaatgggg
    55441 caacatgact gctggaaccg aacttattag acgaaaatca aatggtgtat ctcatgttat
    55501 gactgttcca ttgataacag attctacagg aaaaaaattc ggaaaatctg aaggaaatgc
    55561 tgtttggctt gatgctgaca aaacctctcc ttatgaaatg taccaatttt ggctcaatgt
    55621 tatggatgct gatgccgttc gcttccttaa gatttttaca ttcctaagcc ttaaagaaat
    55681 agaagatata cgtattcaat tcgaggaagc acctcaccaa cgcttagcac aaaaaacact
    55741 agcacgtgaa gttgtaactc ttgttcatgg tgaaaaagct tataaggagg ccgttaatat
    55801 cacggaacag ctatttgctg gcaatataaa aggattgtct gttaaagagc tcaaacaagg
    55861 tttacgtgga gtaccaaatt atcacgttca gacggaagat aacctcaata tcatagacct
    55921 tctagtcact tcaggagttg tcaattctaa acgtcaagct cgagaagatg tctccaatgg
    55981 agctatttat attaatggag atcgtattca agatttagaa tatactatct cagaaaatga
    56041 taagcttgaa aatgaaatta cagtcattcg tcgtggaaag aaaaaatact ttgtattaaa
    56101 ctttaaatag acttgaagtg taatcgatac tctgataata a
//
LOCUS       DATROD010000005        47241 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.005, whole genome shotgun sequence.
ACCESSION   DATROD010000005 DATROD010000000
VERSION     DATROD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 47241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47241
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.005"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(141..314)
                     /gene="secE"
                     /locus_tag="VBC74_000280"
     CDS             complement(141..314)
                     /gene="secE"
                     /locus_tag="VBC74_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEO6941261.1"
                     /translation="MFVKGIFQVLRDTTWPNRKQRWKDFISILEYTVFFTIVIYIFDK
                     LLAAGVMDLINRF"
     gene            complement(350..502)
                     /gene="rpmG"
                     /locus_tag="VBC74_000281"
     CDS             complement(350..502)
                     /gene="rpmG"
                     /locus_tag="VBC74_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO6941262.1"
                     /translation="MAQKKASLACTECGSRNYSIGVSSTPKPTRLEVNKFCKYCKKYT
                     IHKETR"
     gene            complement(551..2872)
                     /locus_tag="VBC74_000282"
     CDS             complement(551..2872)
                     /locus_tag="VBC74_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEO6941263.1"
                     /translation="MKLFDKFIDLFRVDEDNDEMTRKNEQETREETSNLDGEEVYDID
                     DITRPSKSQYQRGIRHQKENAKSRLEWLQKVDRYLPSPKNPIRRFWRRYRIGKLLFIA
                     LMAFILIFGSYLFYLSKTATVSDLQSALKTTTTIYDKNKEYAGKLSGQKGTYVELNAI
                     SDHLKNAVIATEDRTFYENNGVNFKRFFLAVATLGKFGGGSTITQQLAKNAYLSQDQT
                     IKRKAREFFLALELTKKYSKAEILTMYLNNSYFGNGVWGVEDASRKYFGTSAANLTVD
                     EAATLAGMLKGPEVYNPYYSVENATNRRDTVLAAMVDAGQLTKSQAKEAASIGMKNRL
                     ADTYAGKINDYKYPSYFDAVVNEAIDTYGISEKDIVNNGYKIYTALDQNYQSGMQKTF
                     DDTSLFPVSDYDGQSAQGASVALDPKTGGVRGLVGRVQSTKDAQFRSFNYATQSKRSP
                     ASTIKPLVVYSPAIASGWSIDKELPNKVQDFHGYKPSNYGGIETESIPMYQALANSYN
                     IPAVYTLDKLGINKAFTYGRKFGLNMSSANKELGVALGGSVTTNPLEMAQAYSTFAND
                     GIMHRAHLITRIETANGKLVKQFTDKPKRVISRSVASKMTSMMLGTFSNGTAINANVY
                     GYTMAGKTGTTETDFNPNLSGDQWVVGYTPDVVISQWVGFKNTDKHHYLTDSSAGTAS
                     NIFSTQASYILPYTKGSSFTHIENAYFQNGIGSVYNAQDASNTTNQESRSIINDLKDS
                     ASKAAQDISRAVEDSNFQEKVKDAWNSLKDYFR"
     gene            2916..3800
                     /locus_tag="VBC74_000283"
     CDS             2916..3800
                     /locus_tag="VBC74_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO6941264.1"
                     /translation="MSFSLKIRNPYGEHTVKELLEDYFLIPRKIRHFLRVKKHVLINN
                     EFINWQTVVQENDTITLIFDDEDYPTKKIPLGRAELIDCLYEDEHLIIVNKPEGMKTH
                     GNQPNEIALLNHVSAYSGQTCYVVHRLDMETSGAVLFAKNPFILPLINQRLERKEIWR
                     EYWALVEGKFSPKHQVLRDKIGRNRHDRRKRIIDSKNGQHAMTIIDVLKYIQNSSLIK
                     CRLETGRTHQIRIHLSHHGHPLIGDPLYNPSSNNERLMLHAHRLTLSHPLTCETISVE
                     APSSTFEKVLNNYKKGVG"
     gene            complement(3825..5465)
                     /locus_tag="VBC74_000284"
     CDS             complement(3825..5465)
                     /locus_tag="VBC74_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941265.1"
                     /translation="MFDFFKKKVVKVCLVIFGIVLVSLLSLGFFYFSKGQVLSRFVAA
                     RSRTSGQAFDNIKEYMVWSDTGESITNDEANYANFEPLSKSEARKLGQEIKEGNKNDS
                     MYLKRVGSRLGIFPDYRIANKPMSLTLKTNVPKLDVLLNQKKVATSNSDHFSVTVERL
                     PRTHYTASLEGTSDGKEIKLKKDYDGKNQTIDLSVAFKSFTVTSNLMDGNLYFGDNRI
                     AKLKDGSYSVENYPVTDGSKAYIKKVFNDGEITSHKQKLISIADNQTIKLDVDGLLNE
                     KEAGQKLITAFNQLILYVSTGQDPQTLGTVFEKGAENDFYKGLKESIKAKFVTDNRKA
                     SHFTIPNIVLNKMTQVGKESYQVNFAADYDFNYDKSTDPDKKTYGHIIQNLTGNFIMK
                     KSGNSYLISNDGKKDITVAKETNKVKADPVFIFPENLVGSWKGEVEDGTVTMTFDKDG
                     KVTQKKVYKDSKSKESNHSAKVTKLEDKGNGLYLYQYESGTDTTTFVTGGIGGLKVKY
                     AYGIKIEGNKIIPVIWQTSSDGEFDYHKPLLSKPLTKQ"
     gene            complement(5635..6846)
                     /locus_tag="VBC74_000285"
     CDS             complement(5635..6846)
                     /locus_tag="VBC74_000285"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO6941266.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVGDPGNFTRT
                     ANRHDYAVSPFEDTVLNKLDQAGIDTYAVGKINDIFNGSGINHDMGHNKSNSHGIDTL
                     IKTMGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDERLPEIISAMCDKDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPSFTGNGLIPVGHFADISATVADNFGVDTA
                     MIGESFLQDLV"
     gene            complement(6913..7584)
                     /gene="deoC"
                     /locus_tag="VBC74_000286"
     CDS             complement(6913..7584)
                     /gene="deoC"
                     /locus_tag="VBC74_000286"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEO6941267.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECQDAIKNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAACQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVDEGVKIKAAGGISSLEDAEKFIALGASRLGTSRIIKIVKNQKV
                     EEGTY"
     gene            complement(7614..8816)
                     /locus_tag="VBC74_000287"
     CDS             complement(7614..8816)
                     /locus_tag="VBC74_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEO6941268.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQVVSVVSTGVTKVINCGQAGLNFVFGSLADSGTKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILFPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRGTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            complement(8837..9616)
                     /gene="udp"
                     /locus_tag="VBC74_000288"
     CDS             complement(8837..9616)
                     /gene="udp"
                     /locus_tag="VBC74_000288"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEO6941269.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            9775..10512
                     /locus_tag="VBC74_000289"
     CDS             9775..10512
                     /locus_tag="VBC74_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6941270.1"
                     /translation="MPKNELNNKLNKLKHVQVYNKIFNMIQDGTYSPGMQLPSEPELA
                     GQLNVSRATLRKSLALLQEDHLVKNIRGKGNFIRENSSNLSENGYENRQHPIKTCLTS
                     KITEVELEFRVEVPAEAITASLKQETPVVVIADRWYHTDDGPLAYTLSFIPIELISDA
                     EISLHDTKQLLNFIEEGIYHEGISSHSQSHLGYATSGNFSATKYTLSDHGQFILIQET
                     IFNQEKILMCNKHYVPIEHFELSITSH"
     gene            10525..10889
                     /locus_tag="VBC74_000290"
                     /pseudo
     CDS             10525..10889
                     /locus_tag="VBC74_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181221.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10921..12543)
                     /gene="groL"
                     /locus_tag="VBC74_000291"
     CDS             complement(10921..12543)
                     /gene="groL"
                     /locus_tag="VBC74_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEO6941271.1"
                     /translation="MAKDIKFSADARSAMVRGVDILADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPIGIRRGIETAVSAAVEELKEIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVSELENPYI
                     LITDKKISNIQEILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVVTEDLGLDLKDATMQVLGQSAKVTVDKDSTVIVEGA
                     GDSSAIANRVAIIKSQMEATTSDFDREKLQERLAKLAGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVSGGGTALVNVIEKVAALKLNGDEETGRNIVLRALEEPVRQ
                     IAYNAGYEGSVIIERLKQSEIGTGFNAANGEWVDMVTTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPEAPTAPAMDPSMMGGF"
     gene            complement(12639..12923)
                     /gene="groES"
                     /locus_tag="VBC74_000292"
     CDS             complement(12639..12923)
                     /gene="groES"
                     /locus_tag="VBC74_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEO6941272.1"
                     /translation="MLKPLGDRVIISFVETEEKSVGGFVLAGASHDTTKTAKVLAVGD
                     GIRTLTGELVAPSVAEGDTVLVENGAGLEVKDGNEKVTVVRESDIVAVVK"
     gene            complement(13098..13901)
                     /locus_tag="VBC74_000293"
     CDS             complement(13098..13901)
                     /locus_tag="VBC74_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6941273.1"
                     /translation="MSLLELVNLHKTFEKGTVNENHVLRGLDLTIEDGDFISVIGGNG
                     AGKSTLLNCIAGLIPIDQGTITLDNQSITKDSVEKRSKGISRVFQDPRMGTATNLTIE
                     ENMAIAHKRGNKRHIFRQSVTDDDRQLFKKSLSQLGLGLENRMKTDAAFLSGGQRQAL
                     TLAMATLVRPKLLLLDEHTAALDPKTSDMVMELTQKVIEEQRLTALMITHNMEHAIAY
                     GNRLVMLYHGKIVVDVKGEAKRNLTVAELMELFHKNSGQQLIDDALVLG"
     gene            complement(13906..15780)
                     /locus_tag="VBC74_000294"
                     /pseudo
     CDS             complement(13906..15780)
                     /locus_tag="VBC74_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364177.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(16245..17042)
                     /locus_tag="VBC74_000295"
     CDS             complement(16245..17042)
                     /locus_tag="VBC74_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6941274.1"
                     /translation="MVRLIFSDIDGTLINSNFKVTPKTRQGIKQIVAQGATFVPISAR
                     MPEAITPIMEQIGIDSYIISYNGALIQDMQQKTIASHTMDGQVALQVCSYVSKHYSKI
                     AWNVYRYHEWYSCDKENEWVQKEEEIVGLQSKEMSLMELEKQDRIHKLLLMGEPSLMG
                     ELENTLKAQYPHLSIAQSAPYFIEIMAPGIEKGKSAKTLADYLDISLADSIAFGDNYN
                     DLNLLEIVGKGFVMGNAPKDLQERIGNVTQDNDNDGIYYALVEYMEN"
     gene            17228..18088
                     /locus_tag="VBC74_000296"
     CDS             17228..18088
                     /locus_tag="VBC74_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6941275.1"
                     /translation="MPYPYKANHSIESITLKVNDLENLVNFYSDIIGLTVIDKSSTRA
                     LLGVNQKIPLIILEKTELEKHSTYGLYHTAILVPDEYHLSLALNHLLSQHIPLEGGAD
                     HGYSNAIYLSDPEGNGIEIYNDKDISMWDIRESGQIIGITERLDIDNLLDSLVNVPNN
                     YKLSEKTRIGHIHLSVKDAKISSKLYQNVFGLDEKFAIPTASWIASGNYHHHLAFNNW
                     AGPNLSKNQENRPGISLLTIAYNDDNLFRDSLKKAQLYQLTFLEKQDHYYIIEDFDGI
                     RIKVVLKPEN"
     gene            18131..18862
                     /gene="yaaA"
                     /locus_tag="VBC74_000297"
     CDS             18131..18862
                     /gene="yaaA"
                     /locus_tag="VBC74_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEO6941276.1"
                     /translation="MIKILIPTAKEMKVCQNIARPKLSAQTQIIIDYFSTLTVSDLED
                     IYRINTSAARCEAQRWQDFKCKQLTLNPAIKLFNGLMYRNIKRHNLSTSEAQFMENSV
                     FITSALYGIIPAMTLISPHRLDFNTKIKINNNSLKVFWRENYDTFMQSDDIMVSLLSN
                     EFKTVFSPKERQKLIHLNFIEDRDGQLKTHSTISKKARGKCLTSMMENNCQTLEHLKQ
                     LRFDGFCYDNELSDSKQLTFVKKQT"
     gene            complement(19219..19836)
                     /gene="nrdG"
                     /locus_tag="VBC74_000298"
     CDS             complement(19219..19836)
                     /gene="nrdG"
                     /locus_tag="VBC74_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEO6941277.1"
                     /translation="MSEEASWNTPKPGEWKSEELSHGHIIDYKAFNFVDGEGVRNSLY
                     VAGCMFHCKGCYNTATWSFRAGIPYTKELEDQIMKDLAQPYVQGLTLLGGEPFLNTGI
                     LLPLLQRIRRELPEKDIWSWTGYTWEEMMLETQDKLEMLSLIDILVDGRFDQSKRNLM
                     LQFRGSSNQRIIDVQKSLKEGEVVIWEGLNDGDNSYEQVKRDYLL"
     gene            complement(19909..20400)
                     /locus_tag="VBC74_000299"
     CDS             complement(19909..20400)
                     /locus_tag="VBC74_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6941278.1"
                     /translation="MKLRRPVLEDKEEILAMYKEFQKESSSMDGGFYEPTTHFEDWLD
                     HNLNMELGIGVPDNFVPYIQFVSFDNDNNAIGFLNLRLRLNDTLLEKGGHIGYSIRPR
                     QRGKGYAKEQLKLGIEQAHLKNINEILVTCHVDNDASKSVILANGGILEDCRHQTERY
                     WIT"
     gene            complement(20409..21341)
                     /locus_tag="VBC74_000300"
     CDS             complement(20409..21341)
                     /locus_tag="VBC74_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6941279.1"
                     /translation="MLQLGIVGLGGISQKAYLPYMRQVTGVHWHLFTRQKQILEEVNM
                     LFGSSTAYDSLDSLAEHPLDGVFIHVATSAHFDIAKLFLKKGIPVFMDKPLTEDYTST
                     KALYDLAKDHKTFLMAGFNRRFAPRIMEMKKVEDKNHIRTFKNAVNAPADFQYKLFDM
                     FIHPLDTALFLTNNVVKRGYFVTKRDGNKILQVSVTLETDSEIIEASMNLQSGSRREI
                     IEIESPEVTYSLDDLSNLSVIDGFDRRAIGFGSWASTLEKRGFEPMIDAFIQAITTGV
                     NPISPKSSLLSHFICDQINKANAPFGMLNLKIME"
     gene            complement(21354..21497)
                     /locus_tag="VBC74_000301"
     CDS             complement(21354..21497)
                     /locus_tag="VBC74_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941280.1"
                     /translation="MGKYQLDYKGQAQVQKFHEKHSTGENANQKSRLKDLRKQFLEKA
                     KKK"
     gene            complement(21572..23770)
                     /gene="nrdD"
                     /locus_tag="VBC74_000302"
     CDS             complement(21572..23770)
                     /gene="nrdD"
                     /locus_tag="VBC74_000302"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEO6941281.1"
                     /translation="MMVLERERFMTESDIKVIKRDGRLVSFDKYKIYTALLKASNKVI
                     KMSPLVEAKLEMIADHVIAEIYNRFKDNIKIYEIQNIVEHKLLEANEYAIAQEYINYR
                     TQRDFERSQATDINFSIGKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAQLNYQKHLKDAKEWVIEDKQEDYAR
                     AKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTNWFEREIQKAILKIRIQGL
                     GSEHRTAIFPKLIFTLKKGLNLEENSPNYDIKQLALECATKRMYPDVLSYDKIIDLTG
                     SFKAPMGCRSFLQGWRDANGQDVTSGRMNLGVVTVNLPRVAMESNGDMDKFWEIFNER
                     MSIARDALVYRVERVKEAIPANAPILYQYGAFGERLGKYENVDRLFNHRRATVSLGYI
                     GLYEVASVFYGGDWEDNHQAKAFTVDIVRKMKQLCADWSDEYDYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKNPTPFEKLDFEKIYPETGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYQCQFEGDFTPTDRGFTCPNCG
                     NSDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSSIKNQGN"
     gene            complement(23869..25410)
                     /locus_tag="VBC74_000303"
     CDS             complement(23869..25410)
                     /locus_tag="VBC74_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941282.1"
                     /translation="MVENLEKPIGVSYKNSPSMSKRTAIRMKKSSRFSILLYSVLSTL
                     LAIANPLLTYFANGLQTQNLYTGLMMTKGQIPYSDVFATGGFLYYVTIALSYLLGSSI
                     WLFIVQFIAYYVSGIYFYKLVYYVAQSEIVSIGMTLIFYIMNIVLGFGGMYPIQWALP
                     FMLISLWFLIKFCVDNIVDEAFIFYGILAAFSLFIDPQTLIFWLCSFVLLTATNIKQK
                     QSLRGFYQFLCVVFGMILIAYTVGYFMFNLQIISSYIDKAIFYPFTYFARTNHSFLLS
                     LAIQIVVLLGSGCLFGLWDFIQNRKKASYQIGLNFIACIFIIYAIMAIFSRDFNLYHF
                     LPALPFGLLLTSNKITILYQKVIDRRSHRRQYFSGKSLIVDLFVKKTYYLPLLLVSLL
                     IGLLVYNTYQNVTLSKERRDISHYLTTKIDRDGKIYVWDKVASIYSQTRLKSASQFVL
                     PHINTAQKNNEKILKDELLQHGAKYFILNKNEKLPNELKSDIKKHYQEVPLSNITHFV
                     LYRFK"
     gene            complement(25661..25978)
                     /locus_tag="VBC74_000304"
     CDS             complement(25661..25978)
                     /locus_tag="VBC74_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEO6941283.1"
                     /translation="MAHNHNHGHNHEHQHEVITLVDENGNETLFEILLTIDGREEFGK
                     NYVLLVPAGAEEDEQGEIEIQAYSFTENADGTEGDLQPIPEDSDAEWDMIEEVFNSFL
                     DEE"
     gene            complement(26004..26423)
                     /gene="ruvX"
                     /locus_tag="VBC74_000305"
     CDS             complement(26004..26423)
                     /gene="ruvX"
                     /locus_tag="VBC74_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEO6941284.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEESGNFGFDR
                     LAELVKEYKVDKFVVGLPKNMNNTSGPRVEASQVYGDKITELFNLPVEYQDERLTTVQ
                     AERMLVEQADISRGKRKKVIDKLAAQLILQNYLDRMF"
     gene            complement(26423..26689)
                     /locus_tag="VBC74_000306"
     CDS             complement(26423..26689)
                     /locus_tag="VBC74_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEO6941285.1"
                     /translation="MGFTDETVRFRLDDSNKVEISETLTAVYRSLEEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDL"
     gene            complement(26891..27289)
                     /gene="spx"
                     /locus_tag="VBC74_000307"
     CDS             complement(26891..27289)
                     /gene="spx"
                     /locus_tag="VBC74_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEO6941286.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHQLPYKEQNLGKESLTRDEILE
                     ILTKTESGIESIVSSKNRYAKALNCNIEELSVNEVIDLIQENPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNVENAEARLRAAL"
     gene            complement(27505..28644)
                     /gene="recA"
                     /locus_tag="VBC74_000308"
     CDS             complement(27505..28644)
                     /gene="recA"
                     /locus_tag="VBC74_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEO6941287.1"
                     /translation="MAKKTKKAEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIVEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYSSVRLDVRGNTQIKGTGEHKDHNVGKETKIKVVKNKVAPPFREAF
                     VEIMYGEGISRTGELIKIASDLDIIQKAGAWYSYNGEKIGQGSENAKKYLADNPAIFD
                     EIDHKVRVHFGMTEDDSPVQSELVEEKNEADDLVLDLDNAIEIEE"
     gene            complement(28718..29977)
                     /locus_tag="VBC74_000309"
     CDS             complement(28718..29977)
                     /locus_tag="VBC74_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEO6941288.1"
                     /translation="MRAEIIAVGTEILTGQIVNTNAQFLSEKMAELGIDIYFQTAVGD
                     NEERLLSLLDIASQRSQMVILCGGLGPTEDDLTKQTLAKFLGKSLTVDLLASQKLDRF
                     FASRPQFARTPNNERQAQLVEGSIPLQNLTGLAVGGIVTSKGVQYMVLPGPPSELKPM
                     VMEQVVPILSNNGTKLYSRVLRFFGIGESQLVTILEDIIKNQTDPTIAPYAKIGEVTL
                     RLSTKAENQNEADFKLDSLEKEILALKTLDNRKLKDLLYGYGDNNSMARTVLELLKVQ
                     NKTITAAESLTAGLFQSQLAEFSGASQVFNGGFTTYSMEAKSQLLGIPKKKLQEYGVV
                     SHFTAEAMAQQARQLLKADFGIGLTGVAGPDELEGYPAGTVFIGIATPEGVSSIKVSI
                     GGKSRSDVRHISTLHAFDLVRRALLKI"
     gene            complement(30066..30617)
                     /locus_tag="VBC74_000310"
     CDS             complement(30066..30617)
                     /locus_tag="VBC74_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEO6941289.1"
                     /translation="MKRCSWVNLDNPLYVAYHDKEWGRAVHDDHVLFELLCLETYQSG
                     LSWETVLNKRQEFRQVFHHYNIEKVAAMSDADLEIILQNPRVIRHRLKLFSTRQNARS
                     IILIQKEFGSFDRSIWSFVDNKVQVNSVNNYNDVPASTTLSERLSKDLKKRGFKFVGP
                     TCLYSFIQAAGMVNDHENICDFK"
     gene            complement(30640..31230)
                     /gene="ruvA"
                     /locus_tag="VBC74_000311"
     CDS             complement(30640..31230)
                     /gene="ruvA"
                     /locus_tag="VBC74_000311"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEO6941290.1"
                     /translation="MYDYIKGKLSKITAKFIVVETAGLGYVIYVANPYSFSGYVNQEV
                     TIYLHQVIRDDAHLLFGFHTENEKEIFLNLISVSGIGPTTALAIIAVDDNEGLVSAID
                     NSDIKYLTKFPKIGKKTAQQMILDLSGKFVEASGESATSRKVSSEQNSNLEEAMEALL
                     ALGYKATELKKVKAFFEGTNDTVEQYIKSSLKMLMK"
     gene            complement(31232..32464)
                     /locus_tag="VBC74_000312"
     CDS             complement(31232..32464)
                     /locus_tag="VBC74_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6941291.1"
                     /translation="MKEFFALPKQLQLRELLRFISITVGSAIFPFMAMYYVQYFGNLV
                     TGILIIITQLSGFVATLYGGHLSDAIGRKKVVIVGSLLATIGWAITIAANVPNHITPH
                     LTFVGILIIEIAHQFYSPAYEAMTIDLTNEQNRRFVYTIGYWLVNIAVMLGSGIAGIF
                     YDHHFFELLIVLLIISAICCLVVYFKFDETKPQEGTFKHDKGVLGTFKNYSQVLVDKA
                     FVVYTLGAIGSSVVWLQVDNYFSVNLKQNFEVVSVLGHTITGAKMLSLAVFTNTLLIV
                     LLMTTINKFIENWPLKRQLILGSLICGFGMLFNISLNTFGAILIAMTFFTFGEMIYVP
                     ASQVLRAEMMVEGKIGSYSGFLAIAQPVASVLAGAMVSLSYFTGKIGVQITLTIFMLA
                     GLVLILYATKMKNIEIGK"
     gene            complement(32496..34469)
                     /gene="mutL"
                     /locus_tag="VBC74_000313"
     CDS             complement(32496..34469)
                     /gene="mutL"
                     /locus_tag="VBC74_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEO6941292.1"
                     /translation="MSKIIELPDILANQIAAGEVVERPSSVVKELVENAIDAGSSQIT
                     IEVEESGLKKIQITDNGEGMTSEDAVLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     IASISLMTIKTATEQGKQGTLLVAKGGNIEKQEVVSSPRGTKILVENLFFNTPARLKY
                     MKSLQSELAHIIDIVNRLSLAHPEVAFTLINDGKEMTKTSGTGDLRQAIAGIYGLNTA
                     KKMIEISNADLDFEISGYVSLPELTRANRNYITLLINGRYIKNFLLNRSILDGYGSKL
                     MVGRFPIAVIDIQIDPYLADVNVHPTKQEVRISKERELMSLISSAISESLKQYDLIPD
                     ALENLAKTSTRSVDKPIQTSFSLKQSGLYYDRTKNDFFIDADTVSEPIANFTNLDKSD
                     GSVDNDVKNSVNQGATQSPNIKYASRDQADSENFIHSQDYLSSKQSLNKLVEKLDSEE
                     SSTFPELEFFGQMHGTYLFAQGNGGLYIIDQHAAQERVKYEYYREKIGEVDNSLQQLL
                     VPFLFEFSSSDFLQLQEKMSLLQDVGIFLEPYGNNTFILREHPIWMKEEEVESGVYEM
                     CDMLLLTNEVSVKKYRAELAIMMSCKRSIKANHTLDDYSARHLLDQLAQCKNPYNCPH
                     GRPVLVNFTKADMEKMFKRIQENHTSLRDLGKY"
     gene            34695..34898
                     /locus_tag="VBC74_000314"
     CDS             34695..34898
                     /locus_tag="VBC74_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEO6941293.1"
                     /translation="MTQGTVKWFNSEKGFGFISSETGTDVFAHFSEIKVDGFKTLEEG
                     QKVTFDIQDGQRGPQATNINLVK"
     gene            complement(34955..37531)
                     /gene="mutS"
                     /locus_tag="VBC74_000315"
     CDS             complement(34955..37531)
                     /gene="mutS"
                     /locus_tag="VBC74_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEO6941294.1"
                     /translation="MAKPTISPGMQQYLDIKENYPDAFLLFRMGDFYELFYDDAVKAA
                     QILEISLTSRNKNAEKPIPMAGVPYHSAQQYIDVLVELGYKVAIAEQMEDPKKAVGVV
                     KREVVQVVTPGTVVESTKPDSANNFLVAIDSQDQQTFGLAYMDVSTGEFQATLLTDFE
                     SVRSEILNLKAREIVVGYQLTDEKNHLLTKQMNLLLSYEDERLNDIHLIDEQLTDLEI
                     SAAEKLLQYVHRTQKRELSHLQKVVHYEIKDYLQMSYATKNSLDLLENARTSKKHGSL
                     YWLLDETKTAMGTRMLRTWIDRPLVSMNRIKERQDIIQVFLDYFFERNDLTESLKGVY
                     DIERLASRVSFGKANPKDLLQLGQTLSQIPRIKMILQSFNQPELDIIVNKIDTMPELE
                     SLINTAIAPEAQATITEGNIIKSGFDKQLDNYRTVMREGTGWIADIEAKERAASGIGT
                     LKIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGEMLEAREQS
                     SNLEYDIFMRVRAQVESYIKRLQELAKTIATVDVLQSLAVVAENHHYVRPKFNDEHQI
                     KIKNGRHATVEKVMGVQEYIPNSIYFDSQTDIQLITGPNMSGKSTYMRQLALTVIMAQ
                     MGGFVSADEVDLPVFDAIFTRIGAADDLISGQSTFMVEMMEANQAVKRASDKSLILFD
                     ELGRGTATYDGMALAQSIIEYIHDRVRAKTMFATHYHELTDLSEQLTRLVNVHVATLE
                     RDGEVTFLHKIEPGPADKSYGIHVAKIAGLPIDLLDRATDILSQLEADAVQLIVSAPQ
                     EAVTADLNEELDPGKQQGQLSLFEEHSNAGRVIEELEAIDIMNLTPMQAMNAIFDLKK
                     LL"
     gene            complement(37588..38025)
                     /gene="argR"
                     /locus_tag="VBC74_000316"
     CDS             complement(37588..38025)
                     /gene="argR"
                     /locus_tag="VBC74_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO6941295.1"
                     /translation="MNKIERQKRIKRLIQSGQIGTQEEIKLHLKNEGIDVTQATLSRD
                     LREIGLLKLRSPEGKLYYSLSTATSNRFSPALRSYILKVSRASFMLVLNTNLGEASVL
                     ANFIDEKGLPEILGTMAGADTLLVICQNEDIAKVFEKELSVGL"
     gene            38247..39938
                     /locus_tag="VBC74_000317"
     CDS             38247..39938
                     /locus_tag="VBC74_000317"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEO6941296.1"
                     /translation="MDTKHLIASEIQKVVPYMEQSAILSLLETPKNSSMGDLAFPAFS
                     LAKTLRKAPQIIASDIAEQIKSDQFEKVEAVGPYVNFFLDKAAISSQVLKQVLSDGSA
                     YATQNIGEGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLANIFDKIGYHPVKINHLGD
                     WGKQFGMLIVAYKKWGNEEAVRAHPIDELLKLYVRINAEAEIDPSVDEEAREWFRKLE
                     ANDPEATELWQWFRDESLLEFNRLYDKMNVTFDSYNGEAFYNDKMDEVLELLESKKLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSIYVVGNE
                     QSAHFKQLKAVLKEMDYDWSDDMTHVPFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKDKVAQAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKANFNPSNSDNYSLNDVESWEIIKLIQDFPRIIVRAADNFEPSIIA
                     KFAINLAQCFNKYYAHTRILDEDAEISSRLALCYATATVLKESLRLLGVDAPNEM"
     gene            40026..40334
                     /locus_tag="VBC74_000318"
     CDS             40026..40334
                     /locus_tag="VBC74_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEO6941297.1"
                     /translation="MPSEKEILDALSKVYSEEVIQADDYFRQAIFELASQLEKEGMNS
                     LLATKIDSLINQYVLTHQFDAPKSIFDLSRLVKTKASHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(40361..41233)
                     /locus_tag="VBC74_000319"
     CDS             complement(40361..41233)
                     /locus_tag="VBC74_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO6941298.1"
                     /translation="MLKLDLKTKIKEAILIAFGVALYTFGFVKFNMANHLAEGGISGV
                     TLIIHALFGVNPALSSLLLNIPLFILGARILGKKSLLLTIYGTVLMSFFMWFWQQIPV
                     TVPLKNDMMLVAVAAGILAGTGSGLVFRYGATTGGADIIGRIVEEKSGIKLGQTLLFI
                     DAIVLTSSLVYINLQQMLYTLVASFVFSQVLTNVENGGYTVRGMIIITKESESAAATI
                     LHEINRGVTFLRGQGAYSGREHDVLYVALNPSEVRDVKEIMADLDPDAFISVINVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(41341..42285)
                     /locus_tag="VBC74_000320"
     CDS             complement(41341..42285)
                     /locus_tag="VBC74_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO6941299.1"
                     /translation="MKTRRTPLEKKVKYIISVWAKKFGLLHTLKSISREKYAEKISAS
                     LLYGILSSVAVNFFFQPGHVYSSGATGLAQVISAVSKHWFSFEIPVALAFYAINIPLL
                     ILSWRKIGHKFTIFTFITVTVSSIFIQLMPQITLTTDPLINAIFGGLIMGAGVGFSFK
                     SRISSGGTDIISLTIRKKTGRDVGSISFIINGIILLFAGLLFGWKYALYSMVTIFVSS
                     RVTDAIFTKQKKMQAMIVTSKPDCVIKRIHRDLHRGVTCINDAEGTYNHEKKAVLITI
                     LTREEFSDFKYLMLKADPKAFVSVAENVHIIGRFVDDD"
     gene            complement(42275..44026)
                     /gene="aspS"
                     /locus_tag="VBC74_000321"
     CDS             complement(42275..44026)
                     /gene="aspS"
                     /locus_tag="VBC74_000321"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEO6941300.1"
                     /translation="MKRSMYAGRVRSEHIGTSITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEEVAASVMATAESLRSEFVIEVSGVVTAREQANDNLPTGEVELKVQELSV
                     LNTSKTTPFEIKDGIEANDDTRMRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDNLEF
                     IDVETPMLTKSTPEGARDYLVPSRVNQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSDQEIQDIVEGMIAKVMKDTKGLEVSLPFPRMAY
                     DDAMNNYGSDKPDTRFDMLLQDLTEIVKEVDFKVFSEASVVKAIVVKDKADKYSRKNI
                     DKLTEIAKQYGAKGLAWLKYVDNTISGPVAKFLTAIEGRLTEALQLENNDLILFVADS
                     LEVANETLGALRTRIAKELELIDYSKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     AETAHELEGDLAKVRAVAYDIVLNGYELGGGSLRINQKDTQERMFKALGFSAESAQEQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKDNIREVIAFPKNNKASDPMTQAPSLV
                     SEQQLEELSLTVESYEN"
     gene            complement(44119..45399)
                     /locus_tag="VBC74_000322"
     CDS             complement(44119..45399)
                     /locus_tag="VBC74_000322"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEO6941301.1"
                     /translation="MKLQKPKGTQDILPGESAKWQYVENVIRNLFKQYHYDEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFHDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PTKMYYIGSMFRYERPQAGRLREFHQVGVECFGSNNPTTDVETIAMGHHLFEDLGIKN
                     VKLHLNSLGSPESRQAYRQALIDYLTPIREQLSKDSQRRLNENPLRVLDSKEPEDKLA
                     VENAPSILDYLDESSQAHFDAVCHMLDALNIPYIIDTNMVRGLDYYNHTIFEFITEIE
                     DNELTICAGGRYDGLVSYFGGPETPAFGFGLGLERLLLILDKQGISLPIENTIDLYIA
                     VLGSEANLAALDLAQSIRHQGFKVERDYLGRKIKAQFKSADTFNAKVIMTLGSSEVDS
                     KEVGLKNNQTRQEVKVSFENIKTDFSSVLKQLGL"
     gene            45619..45801
                     /gene="rpmF"
                     /locus_tag="VBC74_000323"
     CDS             45619..45801
                     /gene="rpmF"
                     /locus_tag="VBC74_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEO6941302.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            45817..45966
                     /gene="rpmG"
                     /locus_tag="VBC74_000324"
     CDS             45817..45966
                     /gene="rpmG"
                     /locus_tag="VBC74_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO6941303.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            46246..46872
                     /locus_tag="VBC74_000325"
     CDS             46246..46872
                     /locus_tag="VBC74_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEO6941304.1"
                     /translation="MRYFMIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIY
                     LGQFLGSVSLIFLSLLFAFVLNYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKD
                     GLRKDNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVTLLTFLVMIYLLVFS
                     AQKLAQVPSVGETLEKYSRWFIAVVYLGLGMYILIENNSFDMLWAVLG"
     gene            46884..47222
                     /gene="cadX"
                     /locus_tag="VBC74_000326"
     CDS             46884..47222
                     /gene="cadX"
                     /locus_tag="VBC74_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEO6941305.1"
                     /translation="MKKDSICQVDVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLAVEELCVCDIACLLNLSMASASHHLRKLANQNILDTRREGKIIYYFIKDE
                     EIRDFFNQLG"
BASE COUNT        15027 a         9506 c         7327 g        15381 t
ORIGIN      
        1 tatttgctta ataatctagt aaaaacaatc aaaagattat ttttttataa tttaatcgga
       61 tatatatgtt attttccaca caaaaaaacc cttagtaaaa tctaagggtt tgggttgttt
      121 ctatctaaaa taatctatta ttaaaatcga ttaatcaagt ccatgactcc tgcagctaac
      181 agtttgtcaa agatgtagat aacgattgtg aaaaatacag tgtattccaa gatggaaata
      241 aaatctttcc aacgttgttt tcgatttggc caagtagtgt cacgcaatac ttgaaaaatc
      301 cctttgacaa acattatcag ttctcctttt tgcagtttgt tacgctatat caacgcgttt
      361 ctttgtgtat tgtatatttc ttacaatatt tacaaaattt atttacttct agtcgtgttg
      421 gcttgggggt gctacttact ccgattgagt aatttcggct tccgcattct gtacacgcta
      481 ggcttgcttt cttttgtgcc ataacgcctc cataagtgtt attttaacat gatttgtata
      541 atcctgcaag ctatctaaag tagtccttta gactattcca agcatctttt accttctctt
      601 ggaaatttga atcttccaca gctctagaaa tatcttgtgc agccttactt gccgaatctt
      661 ttaaatcatt aataattgag cgtgattctt ggttcgttgt attactagca tcttgtgcat
      721 tataaactga accaataccg ttttggaaat aagcattctc aatatgagta aaactagacc
      781 ccttagtata cggtagtata tatgaagctt gcgtgctaaa tatatttgaa gctgtacctg
      841 cactagaatc tgtcaaataa tggtgtttat ctgtgttctt aaagccaacc cattgactaa
      901 taacgacatc aggcgtatag ccgactaccc actggtctcc agataaattg gggttaaaat
      961 ctgtttcggt tgttccagtt ttacctgcca ttgtataacc ataaacattg gcattaatag
     1021 ctgtaccgtt tgagaatgtt cctaacatca tactagtcat tttactagcc actgagcgac
     1081 taataactcg cttaggctta tctgtaaatt gctttactaa ttttccatta gctgtctcaa
     1141 ttcgagtaat caaatgagcg cgatgcatta taccatcatt tgcaaaagtt gagtaagctt
     1201 gtgccatttc taaaggatta gtagttacac ttccacctag cgccactcct agctctttgt
     1261 ttgctgatga catatttaga ccaaacttcc tgccataagt aaaagcttta ttgattccta
     1321 gtttatctaa agtataaaca gcaggaatat tataagaatt agctagtgct tgatacatcg
     1381 ggatactttc agtctcaatt ccaccatagt tacttggttt ataaccatga aaatcttgaa
     1441 ctttattagg aagttcttta tctattgacc atcctgaagc aatagctggt gaataaacaa
     1501 ctaaaggttt tatggttgag gctggacttc tctttgattg agtcgcgtag ttaaaacttc
     1561 taaactgtgc atctttagta ctttgaacac gtccaactaa acctcttaca ccaccagttt
     1621 ttggatctag agctacgcta gcaccttggg cagattgacc atcatagtca gatacaggga
     1681 agaggctagt atcatcaaat gttttttgca ttcctgattg ataattttga tctaatgcag
     1741 tgtaaatctt atagccatta ttgacaatat ctttttctga aataccataa gtatcaatgg
     1801 cttcattgac aacagcatca aaataggatg ggtacttgta atcattaatt ttaccagcat
     1861 aggtatctgc taatctattt ttcataccaa ttgaagcagc ttctttagct tgagacttgg
     1921 ttagttgacc tgcatcaacc atcgctgcca aaacagtatc tcttcggtta gttgcatttt
     1981 caacagaata ataaggatta taaacttctg gtcctttaag cattccagca agtgtagcag
     2041 cctcatctac tgtcaaatta gcagcactgg ttccaaaata tttcctacta gcatcttcca
     2101 caccccaaac accattacca aaataagagt tattaagata catggtcaga atctcagcct
     2161 tgctatattt cttagtcaat tctagcgcta aaaagaattc acgcgccttg cgtttaatcg
     2221 tttggtcctg tgacaagtaa gcattttttg ctaattgctg ggtaatagtt gagcctccgc
     2281 caaatttccc aagtgtagct actgccaaaa aaaatcgctt aaagtttaca ccattatttt
     2341 cataaaaagt tctatcttca gttgctataa cagcattttt gagatgatca gaaatagcat
     2401 ttaactctac ataagttccc ttttgtcccg aaagttttcc agcgtattct ttattcttat
     2461 cataaatggt ggtggttgtt tttagagcac tctgtaaatc actaactgta gcagttttag
     2521 aaagataaaa aagatagcta ccaaatataa gaataaaagc cattaaggcg ataaaaagca
     2581 acttcccaat acgatagcgc cgccaaaaac gacggatagg attcttagga gaagggaggt
     2641 aacgatcaac tttttgtagc cattcaagtc gagatttagc attttccttt tggtgacgaa
     2701 tgcctcgctg atactgggat ttactaggtc gggtaatatc atcaatatca tagacttctt
     2761 cgccatccaa attgctagtt tcttctctag tttcttgttc attttttctt gtcatttcgt
     2821 cgttgtcttc atcaacacga aataagtcta taaacttatc aaataatttc atagacttat
     2881 tatatcaatt ttcaataaaa tgctataata aaaccatgtc attttcatta aaaattagaa
     2941 atccatacgg tgaacatacc gttaaagaac tccttgaaga ttattttttg attccacgta
     3001 agattagaca ttttttgcgt gttaaaaaac atgtacttat aaacaatgaa ttcattaatt
     3061 ggcaaactgt cgtccaagaa aacgatacta ttaccttaat ctttgatgat gaggattacc
     3121 ctactaaaaa aattcctctg ggcagagcag agcttattga ttgtctttat gaggatgaac
     3181 atcttattat cgttaataaa cctgaaggta tgaaaactca cggtaaccaa ccaaatgaaa
     3241 tagcactgtt aaatcatgta tctgcctatt ctggacaaac atgctatgtt gttcatcgcc
     3301 tagatatgga gaccagtgga gctgttttat ttgctaaaaa tccatttata cttcccctta
     3361 tcaatcaacg cttagaacga aaagaaattt ggcgtgaata ttgggcttta gttgaaggaa
     3421 aattttcacc taagcatcaa gttttgagag acaaaattgg acggaaccgt catgacagac
     3481 gtaaacgaat cattgattct aaaaacggtc aacatgctat gacaatcatt gacgttttga
     3541 agtatatcca aaatagtagt ctcataaaat gccgactgga aaccggaaga acccatcaaa
     3601 ttcgcattca cttatctcat cacggacatc ctttaatagg agatcccctc tacaaccctt
     3661 cttctaataa tgaaaggtta atgctacacg ctcaccgatt gactctatcc catccattaa
     3721 cttgcgaaac tattagcgta gaggcccctt catctacttt cgagaaggtt ttaaacaatt
     3781 ataaaaaagg agttggataa taattatcca actcctttta atcattattg cttagttaat
     3841 ggcttactga gtagtggttt atggtaatca aattctccat cactagatgt ctgccagatt
     3901 actgggatga tcttattccc ttcaattttt attccatagg cgtatttaac tttaagacca
     3961 ccgatacctc ctgttacaaa agttgtagta tcagtacctg attcatattg ataaaggtag
     4021 agcccatttc ccttatcctc caacttcgta actttagcag aatggttact ttccttagat
     4081 ttactatcct tgtaaacttt tttctgagta actttaccat ccttatcaaa agtcatcgtt
     4141 actgtaccgt cctcaacttc acctttccaa cttcctacaa ggttctctgg aaaaataaaa
     4201 acgggatcag ccttaacttt attagtttcc ttagctactg tgatatcttt tttaccatca
     4261 ttagaaataa ggtaactatt tcccgatttt ttcataataa aattaccagt taaattctga
     4321 ataatgtggc catatgtttt cttatcagga tctgttgatt tatcataatt aaaatcataa
     4381 tcggcagcaa aattaacttg ataactttct ttcccaacct gtgtcatttt attaaggaca
     4441 atattaggaa tagtgaaatg gctcgcttta cgattatcag ttacaaattt tgctttaata
     4501 ctttctttca atcctttgta aaaatcattt tcggcacctt tttcaaaaac tgttcctaaa
     4561 gtctgagggt cttgcccagt tgatacataa agtatcaatt gattgaaagc agttatcaat
     4621 ttctgtccag cttctttttc atttaataaa ccatcaacat ctaatttgat tgtctgatta
     4681 tcagcgatag agattaattt ttgtttatga gaggttatct caccatcatt aaaaactttt
     4741 ttgatataag cttttgaacc atcagtcact ggataattct ctacggaata gctaccatct
     4801 tttaatttag caatacgatt atcaccaaaa taaagattac catccataag attacttgtt
     4861 actgtaaaag atttaaaagc gaccgataaa tcaatagttt ggtttttacc atcataatct
     4921 tttttgagtt taatctcttt tccgtcacta gtcccttcaa ggctagcagt ataatgggtt
     4981 ctaggtaaac gttccaccgt tactgaaaaa tgatctgaat tgcttgtcgc gacctttttt
     5041 tgattcaaca aaacatccaa tttaggaaca ttggttttca aggtcaaaga cataggctta
     5101 ttagcaatac gataatcagg gaatatacct aagcgactac ctacacgctt tagatacatt
     5161 gaatcatttt tgtttccttc ttttatttcc tgccctaatt tacgcgcctc acttttagac
     5221 aaaggctcaa aattagcgta attggcctca tcatttgtga tagattctcc cgtatctgac
     5281 caaaccatgt attctttaat attatcaaac gcttgacctg acgtacgaga acgtgctgct
     5341 acaaaacgac taagtacttg tccctttgaa aaataaaaga atcctagact cagaagtgat
     5401 actaaaacta taccaaaaat aactaagcac actttaacaa cttttttctt aaaaaaatca
     5461 aacatacctt atcctctcat tcactttata ttaaagtaaa ttgcttaaag aaaagttaca
     5521 acactgataa aacagctgta tttgtacttg taaccgctat ttttctatta taacaaaacg
     5581 aaaaaaggat tgaaaccata atccgatttc aacctctaaa atttttgtct atgattatac
     5641 caagtcctgc aagaagctct ctccaatcat cgcagtatca acaccaaagt tatccgcaac
     5701 tgttgctgaa atatctgcga aatgccctac tgggataagg ccgtttcctg tgaaagatgg
     5761 gctataggct aagagaggaa tgtattctct tgtatggtct gtacctgcat aagttgggtc
     5821 attaccatga tcggcagtga tcaaaagtaa gtctttgtca cacattgctg aaatgatctc
     5881 tgggaggcgc tcatcaaact catgaagaca atcacgatag ccatgaggat cacgacggtg
     5941 accatagaga gcatcaaagt caaccaaatt agtaaaggag aagccttttt caaactctga
     6001 taaccccatt gtttttatca aagtatcaat accatgacta ttcgatttgt tatgtcccat
     6061 atcatgatta atgcctgaac cattgaaaat atcattgatt ttaccaacag cataagtatc
     6121 gatgccagct tgatctagtt tatttaatac ggtatcttca aatggtgata ctgcatagtc
     6181 gtgacggttg gctgtacgcg taaagttgcc tgggtcacct acatatggac gagcgataat
     6241 acgaccaagg agagctggac gctccattgt gatactacga gcatattcac aaatacggta
     6301 aagttcttct agagggatga tatcctcatg agctgcaatt tgtaggactg ggtcagctga
     6361 ggtatagatg atgagctcac cagtttccat ttggcgtggt ccgaagtcat caataacagc
     6421 tgtccctgaa taaggtttat tcgcttcacg aataacttta cgtcctgaaa aatcctcaat
     6481 ctttgtgatg atatcttctg gaaaaccatt ccagaaagta tcaaatggct ctgtaatatt
     6541 taagcccatg atctcccaat gccctgtcat tgtatcttta ccaagtgata cttcttgtaa
     6601 cttcgtcgcg tagccacttg gattttcttc tgcaggaacg gttttaagcg cttggggacg
     6661 aggaatatta ccaagtccaa ttttagccat atttggcacc gcaagtccaa ctgtttttga
     6721 aatgtggcca agcgtgtctg aagcaccatc aggaactcct gcattgacaa aatcattggc
     6781 atctggtgcc gctccaatac ccactgaatc taagacaact aagtgaatgc gatcaaattg
     6841 tgacataact tctcctttat caattgaaaa taggaagtca gaccagcatc cttcctattt
     6901 cctttatatt atttaataag tgccttcttc aactttttgg ttcttaacaa tcttgatgat
     6961 acggctagta cccaagcgtg aagctcctaa agcaataaat ttttcagcat cttccaatga
     7021 tgagattcca cctgctgcct taattttaac accttcgtcg acatattttg ccatcacttc
     7081 aacatcttca aatgtagcac ctgctgtcga aaaaccagta gaggttttaa taaagtctgc
     7141 acctgaacgt gtgacaactc cacaaagttc gataagttct tctttagtta attgacatgt
     7201 ctcaacgata acttttaaga tatggtcttg acatgcagct ttgatttgac gaatttcttc
     7261 ttcaacagta tcaaaatccc catttttaac gtctgtcaaa ttaatgacca tgtcaatttc
     7321 atcagcacca tttttaatag catcttgaca ttcaaaaacc ttcgctgcag ttgtactata
     7381 gccatttggg aacccaataa cagtacaaat agctaattta cctgaaacgt actctgctgc
     7441 ttttttgacg taagaggctg gaatacatgc tgaagctgtt tcataagcca ttgcatcatc
     7501 taaaattgtt tggatttctg gccatgttgc tgttgttgct agcaaagtat ggtctaccgt
     7561 ttttaaaata tcttttactt ccacgttact atccttcttt cttttgtcaa cttttagaat
     7621 agagttacaa taccgacgat aaaggcgcta agcatactta cagcaatacc accaatcatt
     7681 gcacggaaaa caagtcgagc tagggtgcca cgtttctctg gacaaagaac agcaatacct
     7741 gaaacacaaa tacctaaact tgataaatta gcaaaaccac agagtgaaat agttgctacc
     7801 aatgctgtac gataatctaa agatttgatt aggtgaccca attgttggaa cgaaacaaac
     7861 tcatttaaaa tcaacttact tccaagaagg tttccttcta gaagaatgtt tttgtggtca
     7921 aatcccataa gaaaaccaaa tggagcaaaa acatatgaga agatttgttc taagcgaatt
     7981 cccaatccac ttaacatcat attaatcaaa gaaactaaac caacaaaggc aatcaaacta
     8041 gcaccaattg agaaagccat ttgtgcacct gtgcttgcac cctcagcgat tgcatcaatc
     8101 acattggcgt tattaccttt attatccatc ttaatgtcat caattttttg aacaggttct
     8161 gtttgaggaa ataagatttt agcaatgaga atactgccaa taggaaccat tgttgaagca
     8221 atcaagagat attccattgg aatgcctaat gcaatgtagc caccaagaat agaaactgac
     8281 atacttccca tacctgatac caacacaacc attatctcac tatcagtcat acgacctagg
     8341 tatttgctaa ccaaaattgg actgtctgtt tgaccaagaa acatattagc tacggcaaca
     8401 aaactctcaa cctctgagga tttcataatt ttaccaacac ccttacctat ccattttact
     8461 acaaatccaa ggattcctac ataataaagt agactaacta gggcagataa gaaaacaata
     8521 ttaccaagcg tttgaatggc gaaaataaaa ccagtttttg ttccactatc tgctaatgaa
     8581 ccaaacacaa aatttaaacc agcttgacca cagttgatta ctttagtaac tccagttgaa
     8641 acaacactaa caacttgttg gcctagtggg atacgtacta agattagcgc aataatgaat
     8701 tgaacgataa gagcttttcc aattaaactt agagaaacac tcttacgatt gaaagaaatt
     8761 gcatacacaa ttcctaatac caataaaata ccaataatac tataaataaa ttgcatgatg
     8821 acttccaaaa cctcgcttat tgtgatttat cattttcaat aagtgtgcgg agtgcttcaa
     8881 cagcaacttg aatagctgct tctgtgtcat gagccattgg gttatccata cccaaagcgt
     8941 tgcgttcttg attaccaaca acaaggaaat ctgatccaca acgaactcct agatgacttg
     9001 ctgctacaaa aagagctgca gattccattt cagacgcttt tgttcccaga cgtttccatg
     9061 cttcccattt atttaataat tcataactaa ctggcatacg ttctggctca tgttgtccat
     9121 aaaatgcatc tttacattga actactcctg cgtgacttgt gtaaccaagc tttttagctg
     9181 cattaactag agcattcgtt acctccaagt cagctacagc aggaaattca atcggagcat
     9241 attctttact tgtaccttcc atacggatag ctccagttgc aataacgatg tctccgcctt
     9301 taacatcaag gtcaatacca ccacatgttc caacacggat aaaggtatca gcgccacaga
     9361 gtttcaattc ttccatcgca atagacgctg atggaccacc aataccagtt gaagtaacac
     9421 tgactttttc accatttaat gtacctgtat aagtaacata ttcacggctg tctgctacaa
     9481 gaactgcatt atcaaaatgt tctgcaattt ttgcacaacg ttttggatca cctggcatga
     9541 taacataacg acctacatca cctggacgaa tctgtaggtg atattgcaat ccgacttcac
     9601 ctgaataatt ctgcatgata aagacctcct taatgtcttg tctatttttt ttacacttgt
     9661 ataataacat acaagacaac ctttgtaaac gcttttatcg aaagaaaacg ttttattttt
     9721 taacttgatt tttctaattt tttcaattaa actaacttgt atacggaggt tacaatgcct
     9781 aaaaatgaac taaataataa actaaataaa ctaaagcatg tgcaagtcta caataagatt
     9841 tttaacatga ttcaagacgg tacttattct cctggaatgc aattgccttc tgagcccgaa
     9901 ttagcagggc aacttaatgt tagtcgagct actttacgaa agtcattagc actattacaa
     9961 gaagaccatt tggttaaaaa tatcagagga aaaggtaatt tcattagaga aaattctagc
    10021 aacttgtctg aaaacggtta cgaaaataga caacacccca ttaaaacttg tctaacttcc
    10081 aaaattacag aagtggaatt agaatttcgt gtagaggttc ctgcagaagc tatcacagct
    10141 tcacttaaac aagaaacccc tgtagttgtt attgctgatc gttggtacca caccgatgat
    10201 ggaccattag cttacacact ttcttttatc cctatcgaac ttatttcaga tgctgagatt
    10261 tctttacatg atactaaaca gttgttaaac ttcattgaag aaggtatcta tcacgaaggc
    10321 atatcaagcc actcacaaag ccatttggga tatgcgacat ctggcaattt ttcagcaact
    10381 aaatacaccc tatccgatca tggacaattc atattaatac aagaaacaat ttttaaccaa
    10441 gagaaaatac taatgtgtaa taagcattac gttcccattg agcactttga attgtccatt
    10501 acctctcatt aaggaggcaa gcctatgcaa aatagactat taaaagttgc ttttttcata
    10561 acttttaccg aattaattat gggcctaata gttggcgctt attttaagtt aagttatttt
    10621 ctagcccctc caaaggaacc gtcaatgggt gctgggtacg gaatgctctt ttctatgtta
    10681 tttatactta ttagttactt aattctatta gctataattc aattcttagc ttatcaatct
    10741 caatctaaga taatagcaat cattgttttg ctactttttt cctcccacta ctggcaaata
    10801 ttagatggcc tatccttatg atcttcttac cgcaagtggt agtgactatc atatctctat
    10861 tatcacaaca taaaactccc ctttgttaaa ggggagtttt atgttgtgat aagttagtga
    10921 ttagaagcca cccatcatag atggatccat tgcaggagct gtaggagctt ctggttcagg
    10981 tttatttgct actactgctt ctgtagtcaa gataagactt gctacagatg ccgcattttg
    11041 aagtgcagaa cgtgttactt tgacaggatc aatgatacct gtggtaacca tatctaccca
    11101 ttctccatta gccgcattaa atcctgtacc aatttcagac tgttttaaac gttcaataat
    11161 aactgaacct tcatatccag cattgtaagc aatttgacga acaggctctt cgagagcacg
    11221 aagaacaata ttacgtccag tctcctcatc accattaagt ttcagtgccg ctactttttc
    11281 aataacgttc acaagagccg tacctccacc tgaaacaata ccttcttcaa ctgcagcacg
    11341 cgttgcattt aacgcatctt cgatacgaag tttcatctct tttaattctg tttcagtcgc
    11401 cgcaccaact ttaattactg ctacaccacc ggctaactta gcaagtcgtt cttgtaattt
    11461 ttcacgatca aaatcagaag ttgtagcctc catctgtgac ttaatgatgg ctacgcgatt
    11521 agcaattgct gatgagtcac cggcaccttc gacaataaca gtagaatctt tatctactgt
    11581 tactttagca gactgtccta aaacttgcat agtagcatct tttaagtcta aaccaaggtc
    11641 ttcagtaacg acagttcctc ctgttaggat agcaatatct tccagcatgg ctttacgacg
    11701 atcaccaaat ccaggcgctt taacagctac gacattgaaa gttccacgaa ttttgttaag
    11761 aacaagcgtt gggagagctt ctccatcaac atcatcagcg atgattagca acggacgatt
    11821 tgttttaaga acctcttcta ataatggtaa aatttcttgg atatttgaaa ttttcttatc
    11881 tgtaataagg atatacggat tctcaagttc agagaccatt ttctcgttat cagttaccat
    11941 atactgtgac aagtacccac ggtcaaactg cattccttcc acaacttcaa gctctgtttc
    12001 catacctcgc gattcttcaa tagtgataac accatcatta cccacgcgct ccatagcttc
    12061 agaaatgtat tccccaactt tttcagaacg tgaagacaca gccgcaactt gagcaatagc
    12121 ttctttgcct gaaactggtt gtgcaatctc ttttagctct tcaactgctg ctgaaacagc
    12181 tgtttcaata ccacgacgaa tgccaatcgg atttgcccct gcagttacat ttttaagacc
    12241 ttcccgtaca atagcttggg tcaaaacagt agcagttgta gtgccatccc ctgcaatatc
    12301 attagttttt gaagccactt ctgacacaag tttagctccc atattttcaa agtgatcttc
    12361 tagctcaatt tctttagcaa ttgtcacacc atcatttgta attaaaggag aaccaaatgc
    12421 tttttcaaga acaacattac ggcctttagg acctaatgtt actttgactg tatcagctaa
    12481 aatatcaaca ccacgcacca ttgctgagcg ggcatctgct gaaaatttaa tatcttttgc
    12541 cataatttaa tacccaggcc taaaatttaa caaagtctac tttctttaca aagcaacttt
    12601 tctctaatag gcctatatcc tttcttgctt aataaaacct atttaacgac agcaacaatg
    12661 tcactttcgc gaacaactgt cactttttcg ttaccgtctt taacttccaa accagcacca
    12721 ttttcgacta aaacagtatc accctctgca acgctaggtg caactaattc ccctgttaag
    12781 gtacgaatac cgtcacccac agcaagaact ttggctgtct ttgtcgtatc atggcttgct
    12841 cctgcaagaa caaaaccacc gacagatttc tcttcagttt caacaaacga aatgatcaca
    12901 cgatcaccta atggttttaa cataataaac gaacctccat acgagatata ttttgatttt
    12961 ttagcattcc tataaagaga gtgctaacta caaaaactat tctatcactt ggtcagaatt
    13021 agtcaagaga aaaggcacgt ttcaaacaag aaaaatttcc ctagttatcc tgtccattca
    13081 ctagagaaat ttatatttta acctaagaca agggcatcat ctatcaactg ttgacctgag
    13141 tttttatgga aaagttccat tagttctgca actgttaagt tcctctttgc ttctccttta
    13201 acatcaacaa caattttccc gtggtagagc atcacgagac gattaccata agcaatggca
    13261 tgctccatat tgtgtgtaat cattaaggct gtcaatcgtt gttcttctat cactttctgt
    13321 gttaattcca taaccatatc gctagttttg ggatctagag ccgctgtatg ttcatccagt
    13381 aataataatt tgggacgaac aagagttgcc atagctaacg tgagggcttg acgctgaccg
    13441 ccagataaaa atgcagcatc agtcttcatt cgattttcta agcctagacc taattgtgat
    13501 agagactttt taaatagttg acggtcatcg tcagtcactg actgtctaaa aatgtgacgc
    13561 ttattgcctc ttttatgtgc aattgccata ttttcttcaa tcgtcaaatt tgttgcagtt
    13621 cccattctag gatcctgaaa gactcgacta atacccttag atcgtttttc tacagagtct
    13681 ttagtaattg attggttgtc taatgtgatt gtcccctgat caatagggat cagaccagcg
    13741 atacagttaa ggagggttga ttttcccgca ccgttacctc caattactga aataaaatca
    13801 ccgtcttcaa tcgttaaatc taaaccacgt aaaacatggt tctcattgac agttcctttt
    13861 tcaaaagttt tgtgcagatt gactaattca agtaatgaca tgttcttatc cttcctttgt
    13921 aagcgtttta gaggctttta ctcctagttt agctcgtaat tctgggacat acaaaatgag
    13981 agccaacaaa attgctgaaa ccaactttat catctgtgca tcaatatcgg ttgttaatat
    14041 gaaaacaata atcatacggt ataaaacaga acctaataca attgaccata accttttacc
    14101 aagaggtaag tattttatca ttacttctgc caagataatt gaagccaatc caataacgat
    14161 tgtacccact cccatattca aatctgcata gccattattt tgcgctaaaa gtgcaccaga
    14221 taaagcaatc aggccattcc caatcatata tcctagcatt ttcatccgat ctactttaat
    14281 actattggct tgtcccatag cctcattatc acctgtcgct cttaaagcta atcctagttg
    14341 ggtatttaaa aagagataca aaattaagat aactaaacct acacaaacaa ttccaattaa
    14401 taaaactgct gaaagtttat ttaaacctaa tcttgttacc attgtaacca aagtattttt
    14461 gagagcaaaa gagacgtttg atcttcctaa aacaagtaaa ttaatagagt aaagtcctgt
    14521 caacgtaata atacctgtta ggagggctgg tattttcatt ttagtatgaa gaaaacccga
    14581 tactagtcct gccaacatac cacctaacat gcccgctatg gttgccacaa ttggattaat
    14641 atcattaacg atacataagg cgcaaactgc agcccccata gggaaagccc cttctgcaga
    14701 gagatcggct atgtctaaga tacgaaaagt gataaacacg ccaattgcca taattgacca
    14761 aagtaatcct tgcgaaattc cggataagat aatatctaac atacaatacc tttctgatta
    14821 tttattattt ttaatacttg aaacatcaat tccaagctct tttgccatat cttcattaac
    14881 tactactttt agattttgag gatattctga aggaacttta gcgacgtctt tacctttgag
    14941 gattttaaca gcttgtcttg ccgtttgaca acctaaagcc tcataatcag ccccataaga
    15001 aaagagcaaa ccacttggta ccatatctgc tgaacctccc acaacaggca cttttaattc
    15061 tttcgataga ttccctaaca gcgttactga gctggctata atattatctg ttggtacaaa
    15121 aagaacctct gttttagaca ttaaactctt agcagtatct tgtacatcat tagtggaaga
    15181 aataccttta acacttgttt taatccccgc ctcttggaag attttttttg cttgttttac
    15241 ctgaacttct gagttacgct cgctagtggt atacataata ccaacacgtt taaccttagg
    15301 cattactttt ctcaaaaggg atacttgctt tttaattgga gacatgtcac ttgtaccagt
    15361 cgctaaacct tcaggttttt tcattgattt tactaattcg gctgataccg gatctgtcac
    15421 tgctgtaaat aaaataggtg tttctgttga caccgttgtc aacgattgag ccgccggagt
    15481 cgcaattccc aatactaatt tattatcttt aatgagtttt tcagaaattg attggatatt
    15541 tgactgatcc ccttgagcat tcttatactc aattttgata tttttgccat ctttatagcc
    15601 ttctttagct aattctttta taaatccttt cctagtggca gttagagagg gatgttctac
    15661 atattgtaaa ataccaatat gttttacatc agattgacca ttttgagaag attgtgaaca
    15721 agctgtcatc agaacagatg ctaaaacgct gctaattacc atctttgtta tttttttcat
    15781 tactatctcc ttgtaatacc atcaaatttt ccataaaaca aaaaaccaac tagaccctac
    15841 atctagttgg ttaattattt ctctcaattc ctctgtgcac gggagggcga aaaaatattg
    15901 ccttaaccaa ctagattatt gaaatgatct aatcggctct gctttcatca taaaatgctt
    15961 tagcttcata gatacaaact ttgcgtgacg tttgtatcct tgaggctaca aacgtctatc
    16021 taaagaaact aaagtaataa taagtatttg tttgtttaat aatagacgat ttgattaaca
    16081 tattattctc tccttgttta tggttgccta taagtttacg tcatttaaaa ataattgtca
    16141 atagttaaat ttgatttttt tgaaaattct tgttagatag ataatttaaa taatagtcat
    16201 tcgttgatat aacacacttt taaggaaatt tagatttgct atccttaatt ttccatatac
    16261 tctactaaag cataataaat accatcgtta tcattatcct gagttacatt tccaattctc
    16321 tcttgtaaat ctttaggagc attccccatg acaaaacctt tacccacaat ttcaagcaga
    16381 tttaaatcat tatagttatc accaaacgca atagagtctg caagagaaat atctaaatag
    16441 tctgctaaag ttttagcaga ttttcctttt tcaatacctg gagccattat ttcaataaag
    16501 taaggagcag attgtgcgat tgataaatgt ggatattgag ctttcaaagt gttctccaat
    16561 tctcccatca gagatggttc tcccatcaag agaagtttat gaatcctatc ttgcttctct
    16621 aactccatca aactcatctc tttagattgt aaaccaacaa tctcctcctc tttttggacc
    16681 cactcatttt ctttatcaca ggaataccat tcatgataac gatagacatt ccatgcaatt
    16741 ttcgaataat gcttgcttac ataagaacaa acttgtaagg caacttgacc atccattgtg
    16801 tgggaagcaa ttgttttttg ttgcatatct tgtattaaag ccccattata ggaaataata
    16861 taagaatcta ttccaatttg ttccataatc ggagtaattg cctcaggcat cctagctgat
    16921 atgggaacga aagtagcccc ttgagctact atttgtttaa ttccctgccg tgtcttaggt
    16981 gttactttaa aattactgtt aattaaagta ccatcaatat cactaaatat tagccttacc
    17041 atattatcct ccaaaatata attgtacgcg cttactttag tctaacactt aaatttttgt
    17101 ttcgcaaata aacttcgtct ggtctaatga gactactaat ataagctata tttcaaaaca
    17161 aattagttta tttctctcaa tgaacggttt aatatgttac tatatagtaa taataggagg
    17221 attaactatg ccgtatccat acaaagccaa tcattccatt gaatcaatca ccttaaaagt
    17281 aaatgacctc gaaaacctcg taaacttcta tagcgatatc attgggttaa ctgttattga
    17341 caaatcaagc actagagcct tactaggggt aaatcaaaaa atccctctta ttatccttga
    17401 aaaaactgaa cttgaaaagc atagtaccta tggcctttat cacactgcta ttttagtacc
    17461 agatgaatac catctcagtc tagctctaaa tcatttatta tctcagcata tccctctaga
    17521 aggtggagct gaccatggct atagcaatgc tatctatcta agtgatcctg aaggtaatgg
    17581 aattgaaatt tataatgata aggatatctc tatgtgggat atcagagaga gtggacaaat
    17641 tattggtatt actgaaagac tagatattga caatttactt gacagccttg tcaacgttcc
    17701 taataactat aagctttctg aaaaaactcg tattggtcat attcatttga gtgttaaaga
    17761 tgctaagatt agctcaaaac tctatcaaaa tgtttttggc cttgatgaga aatttgcaat
    17821 cccgacagct agttggattg catccggtaa ctaccatcat catctagcct ttaataattg
    17881 ggctggtcca aatctttcta aaaaccaaga aaatagaccc ggaatttcct tattgacaat
    17941 tgcttacaat gatgacaacc tttttagaga tagcttaaaa aaagcgcaac tttaccaact
    18001 gactttcctt gaaaaacaag atcattatta tattattgaa gattttgatg gcatccgaat
    18061 taaagttgtt ctaaaaccag agaattaacc atcacttgac ttttgactct tcattttaca
    18121 taatgtcaat atgataaaaa ttttgatccc aactgcaaaa gaaatgaaag tgtgtcaaaa
    18181 cattgcaagg cctaaactga gtgcacaaac gcaaattata attgattatt tttctacttt
    18241 aactgtttca gacttagagg atatctacag aattaataca agtgcagctc gttgtgaggc
    18301 tcaaagatgg caagatttta aatgtaaaca acttactctg aatcctgcta ttaagttatt
    18361 caacggtctt atgtaccgca atatcaaaag gcacaactta tccacaagtg aagcacaatt
    18421 tatggaaaat tctgttttta ttactagtgc tctttatggc atcattcctg ctatgacact
    18481 aatatcccct catcgtttag attttaacac taaaatcaaa atcaacaata actctcttaa
    18541 agttttttgg cgtgaaaatt acgatacttt tatgcagtca gatgacatta tggtttcttt
    18601 gctttctaat gaatttaaaa ccgtcttttc tcctaaagag cgtcaaaaac taatacatct
    18661 taactttatt gaagatcgag atggccaatt aaaaacccac tctaccatat caaaaaaagc
    18721 aagaggaaag tgtttaactt ctatgatgga aaataattgc caaactctag aacacttaaa
    18781 gcaattacgc tttgatggct tctgttatga taatgaactc tcagactcta agcaattaac
    18841 ttttgttaaa aaacagactt aagtaactat ttatccacaa tttattcaca caaaaagcaa
    18901 gagataatcg tatctcttgc tttttgacaa acttgtaagg tctaaaaacc tctctatatc
    18961 aaggtttcaa ataatattaa caaaaaataa ttgttatcaa caacttgtgg ataactatgg
    19021 ggatagacag aggataatct gtttatataa agttgattac ttaacagtaa tgtcattgta
    19081 tataccttta gatttcaaaa atacttgtta aattaacaat aataaacgtc aacatcttgt
    19141 cacagactgt caaatactta taaacaagct gtggataaac ataactttca atttctattt
    19201 aaaagttaat ttttgacact aaagtaaata atctcgtttg acttgttcat aactattgtc
    19261 accatcgttt aatccttccc aaatcacgac ttcaccttct tttaaagatt tttgtacatc
    19321 aatgattctt tgatttgaag atcctctaaa ttgtagcatt aaatttcgct tactttgatc
    19381 aaacctacca tccactaaaa tatcaatcaa agataacatc tccaatttat cttgagtctc
    19441 cagcatcatt tcttcccaag tataccccgt ccatgaccaa atatcttttt ctggcaattc
    19501 acgtcggata cgctgaagaa gtggcagcaa aatacctgta tttaagaacg gctctccacc
    19561 taataacgtt aaaccttgga cgtaaggttg tgccaaatct ttcataattt gatcttcaag
    19621 ctcttttgta taagggattc cggctcgaaa ggaccatgta gcagtattat aacacccttt
    19681 acagtggaac atacatccag ctacgtagag tgaatttcta acaccttcac catctacaaa
    19741 attaaaagct ttataatcaa tgatatggcc atgacttaat tcttcgcttt tccactcccc
    19801 aggctttgga gtattccaac ttgcttcttc tgacatttct aacctctcta ttgatatgaa
    19861 aagaaccaga actaactggt ctttttatta ttatcttaaa gtactatctt aggttatcca
    19921 ataacgctct gtctgatgac gacaatcttc taaaatacca ccgttagcta atattactga
    19981 cttgctggca tcattgtcta catggcatgt cacaagtatt tcattgatat tttttagatg
    20041 tgcttgctca attcctagct tcaactgttc tttagcataa cctttaccac gctggcgagg
    20101 tcgtatggaa taaccaatat gtccaccttt ttctaaaaga gtatcattta gtcttaaacg
    20161 taaatttaag aaacctatag cattattgtc attgtcaaaa gacacaaact gaatatatgg
    20221 aacaaaatta tcaggaacac ctatccctaa ctccatattt aggttatgat ctagccagtc
    20281 ttcaaaatgc gtagttggtt cataaaaacc accatccatt gaactacttt ctttttgaaa
    20341 ctctttatac atggctaaaa tttcttcttt atcttctaac actgggcgcc gtaatttcat
    20401 attgtttcct attccattat cttcaagtta agcattccaa aaggagcatt cgctttatta
    20461 atctggtcac aaataaaatg actcaataaa ctagactttg gtgatatagg attgacacct
    20521 gtagtgatag cttgaataaa agcatcaatc attggttcaa aaccacgttt ctctagagtg
    20581 cttgcccaag aaccaaaacc gatagcacga cggtcaaagc catcaataac agaaagattt
    20641 gacaaatcat ctaatgaata ggttacttct ggcgactcta tttcaattat ttctcgacga
    20701 gaccccgact gcaggttcat gcttgcttct attatctcac tgtctgtttc tagcgttact
    20761 gatacttgta atatcttatt cccatcacgt ttagtcacaa agtatcctct tttaacaaca
    20821 ttatttgtca aaaatagcgc cgtatctaaa ggatggataa acatatcaaa aagcttatat
    20881 tgaaaatccg ctggtgcatt gacagcattt ttaaaggtgc gaatatggtt tttatcttct
    20941 acttttttca tttccataat acgtggtgca aagcgacgat taaaacctgc cattaaaaat
    21001 gttttatgat ccttcgccaa atcatacaat gctttagttg aagtataatc ttctgtcaaa
    21061 ggcttatcca taaaaactgg aatacccttt tttaaaaaaa gctttgctat atcaaagtga
    21121 gcactagtcg cgacatgaat gaagactcca tccaaaggat gctcggctag actatctaaa
    21181 ctgtcataag ctgtagaaga accaaaaagc atattaactt cttctaaaat ctgtttttgg
    21241 cgtgtaaaaa gatgccagtg cacacctgtc acttgacgca tataaggtag ataagctttc
    21301 tgtgaaatac ctcctaaacc gacaattcca agttgcaaca tagcatcctc cttctatttt
    21361 ttctttgcct tttccaaaaa ttgtttcctt aaatctttta gacgtgattt ttgattagca
    21421 ttctctccag tagagtgctt ctcatggaac ttctgtactt gtgcttgccc tttgtaatct
    21481 aattggtatt tccccatatc ttaataactc cttctatttt atcttcttat tttaacatat
    21541 aatagaccag aggttttaat agaggtttta attaattacc ttgattttta atacttgatc
    21601 cgttcatatg ttttacacga gcagagattt ccttatgacg accattaacc atcggacgag
    21661 cctgtggatt acctaagtaa ccacatgtac gtttgacaac atctactgtc ttagggtcgc
    21721 tgttaccaca atttggacaa gtaaagcctc gatctgtcgg tgtaaaatcc ccttcaaatt
    21781 gacattggta acacttatca attggtgtat tagtacctag ataacctacc cgatcataag
    21841 catagtccca aactgcttct aaagcttttg gattctgttg caaaacaggg tattcacagt
    21901 agtgaataaa tcctcctgat gcacccgttt caggatagat tttctcaaaa tctagttttt
    21961 caaaaggtgt tggattctta cgcacatcat aatggaatga attagtgtag tattccttat
    22021 cagtaatatc agttactata ccaaattttt cagtatctaa acgacaaaac cggtcagtca
    22081 agctctccga tggtgtcgaa taaactgaaa aatgataatc atactcatca gaccaatctg
    22141 cacataactg cttcatctta cgaacgatgt ctacagtaaa tgctttagct tgatgattat
    22201 cttcccagtc accaccataa aaaacactcg ccacctcata taaaccaata taaccaagag
    22261 acaccgttgc acggcggtga ttaaagagtc gatcaacatt ctcatatttt cctaaacgtt
    22321 ctccaaatgc tccatactgg taaagaatgg gcgcatttgc tgggatagcc tctttgacac
    22381 gttccacacg ataaaccaag gcatctctag caatactcat acgttcatta aatatctccc
    22441 agaatttatc catatcaccg ttagactcca tggcaacacg cggtagatta actgttacaa
    22501 ctccaagatt catacgacca gatgttacat cctgaccatt tgcatctcgc cacccttgta
    22561 ggaaggaacg acagcccata ggggctttaa aagagcccgt taagtcaata attttatcat
    22621 aagaaaggac atctggatac atccgttttg tcgcacactc taatgctaat tgtttaatat
    22681 cataattggg gctattttct tctaaattaa gacctttttt aagcgtaaaa attaattttg
    22741 gaaaaatagc agtacgatgc tcgcttccta aaccttgaat ccgaattttc aaaatcgctt
    22801 tttgaatttc tcgttcaaac caattagttc ccaatccaaa accaagcgaa gtaaatggcg
    22861 tttgcccatt tgaagtaaat aaagtattaa tctcatactc caaagattgc atggcatcat
    22921 atatatcttt ttgtgtttta gcgcgcgcat agtcctcttg tttatcttca ataacccatt
    22981 cttttgcatc ttttaaatgt ttttgataat ttaactgtgc ataaggagct aaaaactcat
    23041 caatgcgatc tgctgtacag ccaccatatt gacttgaagc aacgttcgca ataatctgtg
    23101 aaatctgagc agtcgctgtc tgaatagatt ttggactctc aacttctgca ttaccaatct
    23161 taaaaccatt tgcaagcatt cccttaaaat cgattaaaca gcaatttgtc atgggagtgt
    23221 atgggctgta atctaaatca tggtaatgga tatcaccctt ttgatgtgca tttgctacat
    23281 gagaaggtaa cattttcaga ccaattgatt tccctacaat acctgctgtt aaatcacgtt
    23341 gggtattaaa cacatcagag tctttattag cattctcatt aacaacagtt tgatctttat
    23401 taattaattt tccaatagaa aaattgatat ctgtagcctg tgaacgctca aaatcgcgtt
    23461 gggtacgata attgatatat tcttgtgcaa tagcgtactc attagcttcc aataatttat
    23521 gttcaacgat attttgtatt tcatatatct tgatgttatc tttaaaacga ttgtagatct
    23581 cagcgatgac atgatctgca atcatctcaa gtttagcctc aactaaagga gacattttga
    23641 taactttatt actagctttt aatagagccg tgtagatttt atacttatca aagctgacca
    23701 aacgcccgtc acgtttaatc actttgatat cagattctgt cataaatcgt tctctttcca
    23761 ataccatcat gtgaaatatc ctccagtgac aattatagca tgcaaagaaa aaaaatcaat
    23821 atattgtgtc aagttttttg ctcagacaca atatattgat tttttaatct atttgaaacg
    23881 atatagaaca aaatgagtga tatttgatag aggaacttct tggtagtgct tcttaatatc
    23941 agatttcagt tcatttggta acttctcatt tttatttaag ataaagtatt tagccccatg
    24001 ctgaagcagc tcgtccttca aaattttctc attatttttt tgcgctgtat taatatgtgg
    24061 caaaacaaat tgcgaagcag attttaaccg agtctggcta taaatgctag caactttgtc
    24121 ccaaacataa atcttaccat ctctatcaat ttttgtagtc aaataatgtg aaatgtcacg
    24181 cctttcttta ctaagagtaa cattttgata agtattataa acaagtaacc ctatcaataa
    24241 ggaaactagt aagaggggca aataatatgt tttctttaca aaaaggtcta cgataagaga
    24301 tttaccagag aaatactgac gtctatgtga acgcctgtca atcacttttt ggtaaagaat
    24361 agtaatttta ttactcgtta gcaaaagacc aaatggtaaa gcaggtaaaa agtggtaaag
    24421 gttaaaatct cttgaaaaaa tggccattat agcgtaaata ataaaaatac aggcaataaa
    24481 gttcaagccg atttgataag atgctttttt acgattttga ataaaatccc atagtccaaa
    24541 gaggcaacct gaccctagaa ggacaacaat ttgaattgct aaactaagaa ggaatgagtg
    24601 gtttgtccta gcgaaatacg taaaggggta aaaaatagct ttgtcaatat aagaagagat
    24661 aatctgtaga ttaaacataa agtaaccaac agtataagca attaaaatca taccaaaaac
    24721 aacgcataaa aactgataga accctcgtag tgactgcttt tgcttaatat tggttgctgt
    24781 taagagaaca aaagagcaaa gccaaaaaat cagtgtttgg ggatctataa atagtgagaa
    24841 tgctgctaaa ataccataaa atataaatgc ttcatcaacg atattatcga cacaaaattt
    24901 aattaaaaac catagcgaaa tgagcataaa aggtaatgcc cactgtattg ggtacatacc
    24961 accgaatcct aagacaatat tcattatata gaaaatcaac gtcatgccta tcgagacaat
    25021 ttcactttgt gccacataat aaactaattt ataaaaataa attccagata catagtaagc
    25081 aataaactgt acaataaata accagatact agaccctaaa aggtaactta gagcaatcgt
    25141 tacatagtat aaaaatcctc ctgtagcaaa aacatcacta taagggatct gtcctttggt
    25201 catcatcaaa cctgtataga gattctgagt ctgcaaacca tttgcaaaat aagtcaataa
    25261 aggatttgca attgctaaga gagttgacaa aacactatat agtaaaattg aaaatcgact
    25321 agatttcttc attctgatag ccgttcgttt actcattgat gggctatttt tatagctcac
    25381 tccaattggt ttttctaggt tttctaccac tatttttctc ctcatattta catactacca
    25441 gtatatcaga aaaccttatt tagtcaattt agccaacact tgaccgtaac gaataatatc
    25501 cctaaagtag tggtatcttc tagacatata ttgtttgaga aattcttgtt cttctaatgt
    25561 tagctgagta tctgacatta acatcacctc cttttactac atcatcacta ttattattcg
    25621 aaaaaaaaaa cgactgaaca attgctcaat cgcttcaata ttattcttca tctagaaagc
    25681 tgttgaaaac ttcctcaatc atgtcccatt ctgcatctga atcttctggt ataggctgaa
    25741 ggtctccttc agttccatca gcattttctg taaatgagta agcttgaatc tcaatttcac
    25801 cttgttcgtc ttcttctgca cctgcaggaa cgaggagaac atagtttttg ccaaactctt
    25861 cacgtccatc aatagttagt aggatttcaa aaagtgtttc gttcccattc tcatctacaa
    25921 gagtgattac ttcatgttga tgttcatggt tatgaccgtg gttatgatta tgtgccattg
    25981 tttctccttt ttatgccgaa tgcttaaaac atgcggtcta aataattttg taagattagt
    26041 tgagctgcta atttatcgat aactttctta cgcttaccac gactaatatc agcttgctca
    26101 actagcatac gctctgcttg aacagtcgtt agacgttcat cttgatattc aactggaaga
    26161 ttgaataatt cagtgatttt atcgccatat acctgactag cctctacacg aggaccactt
    26221 gtattattca tatttttagg tagaccaact acaaatttat caactttgta ctcttttact
    26281 aactctgcta agcggtcaaa accaaaattt ccagattctt catcgatctt aatgatttca
    26341 agaccttgtg cagtgaaacc gagtggatca ctaattgcaa cgccaacagt tttcgagccg
    26401 acgtcaagtc ccattattct cattacaaat ctattccatt tccttgtaaa taataacgaa
    26461 cgagctcttc tacaatctca tcacgttcat atttacgaat ttgattgcga gcatcattgt
    26521 aacgaggtac ataagcaggg tccccactca atacgtaacc aacaatttga ttgatgggat
    26581 tgtaaccttt ttcttctaat gaacgataaa cagctgttaa tgtttcacta atttctactt
    26641 tgttactatc atctaatcta aaacgaacag tttcatctgt aaatcccata cctacacctt
    26701 ctttccttta attctttcac ttattataac ttaaataaag tatttgtaca agaaattgat
    26761 aaacactata ttagaaaatc aaaaagggag cacaagaaac caagaggtga ttctttgatt
    26821 tctttactcc ctaccttata ggtgattttt acaggttatc atgtgactct cacttatgct
    26881 tatctaaagc tcaaagtgct gcacgaagac gagcttctgc attttcaacg ttacgaatag
    26941 agcgaggtag aaaagcacga atatcatctt ccttataacc tacttgaaga cgtttgtcat
    27001 caattagaat tggactcttt aaaatacgtg gattttcttg aataagatca ataacttcat
    27061 ttacactcaa ctcctcaata ttacaattga gcgccttagc ataacgattt ttagaagaaa
    27121 cgatactttc aataccactt tcagtttttg ttaatatttc taaaatttca tcacgtgtta
    27181 gtgattcttt acctaggttt tgttctttat atggaagttg atgagcattt agccaagttt
    27241 ttgctttttt acaacttgtg caacttgaaa tagtataaat cttaatcatg tgccttctca
    27301 ctttctatcc catgatagtt atagtttaac atagtcacaa aaatcaaaca tgggacttaa
    27361 gacctatttt ttatcaattt actaataaaa ataaatagat tttagggatt tttaagctta
    27421 tttttagtat aaaaataagt tccaaccact gtcagaactt attttagtta catttattca
    27481 agttcaagat gtatagttgc tattttattc ctcaatttca atagcattgt ccaaatctaa
    27541 aacaagatca tcagcttcat ttttttcttc aacgagctca gactgaactg gcgaatcatc
    27601 ttcagtcatt ccaaagtgca cgcgcacttt atggtcaatt tcatcaaaaa ttgctggatt
    27661 atctgctaaa tattttttgg cattttcaga accttgtcca attttctcac cattatatga
    27721 gtaccatgca cctgcttttt ggataatatc caaatcacta gcaattttaa ttaactcacc
    27781 cgtgcgagaa ataccttcac catacataat ttcgacaaaa gcttcacgga atggtggagc
    27841 aactttattt ttaacaactt tgatttttgt ctctttacca acattatgat ctttatgctc
    27901 accagtcccc ttgatttgag tattaccacg aacatctaga cgaaccgatg aataaaattt
    27961 caaagcacga ccaccaggtg ttgtttcagg gttaccaaac ataacgccta ctttttccct
    28021 tagctggttg ataaaaattg cgattgtctt tgtcttgtta atggatgctg acaatttacg
    28081 cattgcttga ctcatcatac gagcttgcaa accaacgtgg ctgtcaccaa tatcaccatc
    28141 aatctcagca cgaggaacaa gggctgcaac tgaatcgaca acaactaaat caactgcgcc
    28201 tgaatcaatc aatttacctg caatttcaag accttgctca cctgagtcag gttgagacaa
    28261 gagtaactca tcaatattaa ctccaagagc cgcagcatag gctgggtcaa gagcgtgctc
    28321 agcatcaata aaggcagcaa tcccaccttc cttttgggct tgagctacgg catgaagggc
    28381 aaccgttgtt ttaccagatg actctggtcc ataaatttcg acgatacgtc ctttaggata
    28441 tccacctgct cctaaagcga tatctaaagc aagagaacct gaactcatta cttgaacctt
    28501 ttgttcagct cgctcaccta agcgcatcac tgcgccctta ccaaaatctt tttcaatatt
    28561 tttcaaagca tcatctaagg cttttcgacg ttcatcaccg aatttcttag taatttcttc
    28621 tgcttttttc gtttttttag ccaatgtctt ctccttatat ttctatgaat ctaggttgat
    28681 tcaaatttag aaatcgttct actaattata acaaatatta tatctttaat aaagccctac
    28741 gcaccaggtc aaaagcatgt aaagtactga tatgacggac atctgagcgg ctcttccctc
    28801 caatcgatac tttaatagaa gaaacgcctt caggggttgc aatcccaata aaaactgtac
    28861 ctgcagggta tccttccaac tcatcaggac cagcaactcc cgttaaaccg ataccaaaat
    28921 ctgcttttaa aagctggcga gcttgctgag ccattgcctc agctgtaaaa tgactgacaa
    28981 ctccatattc ttgcaatttt ttcttaggga tgccgagaag ttgtgatttt gcttccatac
    29041 tataggtcgt aaagccacca ttaaaaactt gcgaagcacc agaaaattct gctaactgag
    29101 attgaaataa ccctgctgtt aagctctctg cggcagtaat cgttttattt tgtactttca
    29161 ataattctaa aacagtacgt gccatactat tattatcacc atagccatat aataaatctt
    29221 ttaatttacg gttatctaat gtcttaagag caagtatttc tttttctaag ctatctaatt
    29281 taaagtcggc ttcattctga ttttcagctt tcgttgataa ccttaaagtt acctcaccaa
    29341 tctttgcata tggtgctata gttggatctg tctgattctt aattatgtct tctaagattg
    29401 tgactaactg actctcaccg ataccaaaga atctcaatac acgtgaatat aattttgtgc
    29461 cattatttga caaaataggt accacctgct ccataaccat tggctttaat tcactaggcg
    29521 gacctggtaa aaccatatat tggactccct tagatgtaac gataccacca acagctagac
    29581 cagtaaggtt ttgtagaggt attgagcctt caaccaattg agcttgtcgt tcattatttg
    29641 gcgttctagc aaactgagga cgacttgcaa aaaaacgatc caatttttga ctagctaata
    29701 aatccactgt taatgattta cctaaaaatt tagccaaagt ttgtttcgtt aaatcatcct
    29761 ctgtaggtcc aagtccacca cataggataa ccatttgact gcgctgacta gctatatcta
    29821 aaagcgataa taaacgttcc tcattatctc ctacagctgt ctggaaataa atatcaattc
    29881 ccaattctgc cattttctcc gacaaaaatt gtgcgtttgt attaacgatc tgtcctgtta
    29941 aaatttctgt tcctacagcg ataatttcag ccctcacaac ttcaccctct tatctattcg
    30001 aaataatgac tacattttag caaaaaaagt agcagaagtg ctacttaaaa tatatcacat
    30061 tgatattact tgaaatcaca tatattttca tggtcattaa ccatacccgc ggcctgaata
    30121 aaagaataga ggcaagttgg accaacaaat ttaaaaccgc gtttttttaa atccttagat
    30181 aagcgttcgg ataaggtagt acttgcaggc acatcgttgt agttgttaac tgagttaacc
    30241 tgtactttat tatctacaaa agaccaaatg gagcgatcaa aacttccgaa ttctttctga
    30301 attaatataa ttgacctagc attttgtcgt gttgagaaaa gttttagacg atgacgaata
    30361 accctaggat tttgtaagat tatctctaaa tccgcatctg acattgcagc taccttttca
    30421 atattatagt gatgaaaaac ttgtctgaac tcctggcgtt tatttaatac tgtttcccaa
    30481 gataaacccg attgataagt ttctaaacat agtaactcaa agagtacatg atcgtcatga
    30541 acagctctcc cccactcttt atcatggtaa gctacatata atggattatc taaattcacc
    30601 caagaacatc gtttcatgtg aaacctcatt acttctaaat tatttcatca acatcttcaa
    30661 actagatttg atgtattgct ccacagtatc gttcgtacct tcaaaaaagg cttttacttt
    30721 tttcaattca gtcgccttat accccaacgc taacaaagct tccatagctt cttcaagatt
    30781 gctattctgc tcagagctaa ccttacgact agtcgcactc tcaccagaag cttctacaaa
    30841 cttccctgac aaatcaagga tcatctgttg tgctgttttt tttcctatct ttggaaattt
    30901 agtcaaatac ttaatatcac tattatcaat ggcacttacg agaccttcat tgtcatcgac
    30961 tgcaataatt gctaaagcgg tcgttggacc aattcctgat actgaaatta aattaagaaa
    31021 aatttctttt tcattttccg tatgaaaacc aaataaaaga tgtgcatcat cgcgaatgac
    31081 ttgatgtaag tagatggtaa cttcttgatt aacgtaacct gaaaaagagt agggatttgc
    31141 cacatatatc acgtatccca atccagctgt ttccacaaca ataaatttag ccgttatttt
    31201 actcaatttt cctttaatat aatcgtacat tctattttcc tatctcaata tttttcattt
    31261 tggtagcgta taagattaaa accagacctg ctaacataaa aatggtcaat gttatttgaa
    31321 cacctatttt tcctgtaaaa tagctgagcg aaaccatggc acctgctaat acacttgcaa
    31381 caggttgtgc gatagctaaa aatccagaat atgatccaat ttttccctcc accatcatct
    31441 ccgcccttag aacctgacta gctggaacat aaatcatttc gccaaaggtg aaaaacgtca
    31501 tggcaatcaa aatagctcca aaagtgttta gagaaatatt aaaaagcatt ccaaaaccac
    31561 aaatcaagct tcctaatatt agttgacgtt tcaaaggcca attctctatg aatttattta
    31621 tagttgtcat tagtaagaca attaatagag tgttcgtaaa gactgctaaa ctaagcattt
    31681 tagcacctgt gattgtatgt cctaacacag acactacttc aaaattttgc ttaagattga
    31741 ccgagaaata attatcaacc tggagccaga caacactgga accaattgct cctaaagtat
    31801 aaacaacaaa cgctttatcc actaaaacct gactataatt tttaaaagtt cctaaaactc
    31861 ccttatcatg cttgaaggtt ccctcctgtg gttttgtctc atcaaacttg aagtagacca
    31921 ccaaacaaca aatcgctgaa ataataagaa gaactattaa taattcaaaa aaatggtgat
    31981 catagaaaat cccagcaatc ccagaaccta gcattacagc gatgttaacc aaccaataac
    32041 ctatagtgta aacaaagcga cgattctgtt cgttcgtcaa atcaatggtc atagcttcat
    32101 aggcagggga ataaaattgg tgagctattt caatgatgag gatacctaca aaggtcaagt
    32161 gtggcgtaat atgatttgga acatttgcag ctatagttat agcccaacca atggttgcca
    32221 gcagtgaacc tacaatcaca acttttttcc tccctatagc atctgacaaa tggcctccat
    32281 ataaggtcgc tacaaaccct gatagttgag ttatgatgat caaaattcct gtaactagat
    32341 ttccaaaata ttgaacataa tacattgcca taaaaggaaa aatagctgaa ccaaccgtaa
    32401 tggaaataaa acgcaataat tctcttaact gtagctgttt tggcaaagca aaaaactctt
    32461 tcactttttc tcccttcttc cctaacaagt aattatcaat attttcctaa atccctgagg
    32521 cttgtatgat tttcttggat tcgtttaaac attttctcca tatcagcttt tgtaaaatta
    32581 actaaaacag gtcttccatg agggcaatta taaggatttt tacattgcgc taactgatct
    32641 aataaatgac gtgctgagta atcgtctagc gtatggtttg ctttaataga tcgcttgcaa
    32701 gacatcataa tagccaactc tgcgcgatat tttttcactg atacttcatt agtgagcaac
    32761 aacatatcac acatttcata aacgccagac tctacctctt cttctttcat ccaaattgga
    32821 tgttccctta aaatgaaagt attattacca tacggttcca agaaaatacc aacatcttgt
    32881 aataatgaca ttttttcttg taattgaaga aaatctgaac ttgaaaattc aaatagaaat
    32941 ggtactaaca attgttgaag actattatca acctcaccta tcttttcacg gtaatactcg
    33001 tattttacac gctcttgagc tgcatgttga tcaataatat acaacccacc attcccttga
    33061 gcaaataagt aggtcccatg catttgacca aaaaattcga gctctggaaa agtcgaactt
    33121 tcttccgaat ctaatttttc aacaagtttg ttaagtgact gtttactact aaggtagtcc
    33181 tgagaatgaa tgaagttctc actatcagct tgatctctac tagcatactt tatattagga
    33241 gactgcgttg ctccttgatt aacagagttt tttacatcat tgtcaactga accgtcactt
    33301 ttgtcaaggt ttgtaaagtt agcaataggt tcagatacag tatctgcatc tataaaaaag
    33361 tcatttttag tcctatcgta atacaaacca gactgtttta gtgagaagct tgtttgtata
    33421 ggcttatcta cacttcgggt actagttttc gctaaattct ctaaagcatc cggaatcaaa
    33481 tcatattgtt tcagactctc agaaattgct gagcttatta aactcattaa ttctcgttcc
    33541 ttagaaattc tcacctcttg cttagtcgga tgaacgttga catctgctag ataaggatct
    33601 atctgaatat caatgacagc aattggaaat cttccaacca tcaactttga accataacca
    33661 tctagaattg agcggtttaa aagaaaattt ttaatgtaac gaccgttaat taatagggta
    33721 atatagtttc ggttagctcg tgtcaattca ggcaaactta cataacccga aatttcaaaa
    33781 tctaaatctg catttgaaat ctcaatcatt ttttttgcag tatttaaacc ataaatacct
    33841 gcaatcgcct gtctcaaatc gccagtccca ctagttttag tcatttcttt accatcattt
    33901 atcagtgtaa aagcaacttc cggatgcgct agactgagtc tattaacaat atcaataata
    33961 tgagctagtt cagactgtaa actcttcatg tatttcaatc tggcaggcgt attaaagaat
    34021 aaattttcaa ctaaaatttt tgttccacga ggacttgaaa caacttcctg tttttcaatg
    34081 tttccccctt tagcaacaag taacgtccct tgtttccctt gctctgtagc tgtttttatt
    34141 gtcattaaac taatcgaagc gattgatggt agagcttcac cacgaaaacc taaggtccga
    34201 atacgaaaaa ggtcactctg gcttttgatt ttggatgtag cgtgacgtcg taaactgaga
    34261 acggcatcct cactagtcat cccttcaccg ttatccgtta tttgaatctt tttaagacca
    34321 gattcttcaa cctctatggt aatttggcta cttcctgcat ctatggcatt ttcaactaat
    34381 tctttaacga cgctactagg cctttcaaca acctcaccag cagcaatctg gttagctaat
    34441 atatccggta attcaataat tttagacaag ttcatccctc ctttttttat gattttttgt
    34501 aacttttttt gtgatatctt agccattata ccatattcaa aatattaatt tgacaataac
    34561 acaattttta gttgacatca ctcctattac ctgttagact tagggagcgt aataagtttt
    34621 gaagttgtat ttttgtttaa catttttacc ttttttattt tttacagtaa catttttaga
    34681 aaggtaataa acaaatgaca caaggtacag tgaaatggtt taactcagaa aaaggttttg
    34741 gctttatttc tagcgaaact ggaacagacg tcttcgctca tttttcagag attaaagttg
    34801 atggcttcaa aactcttgaa gaaggacaaa aagtaacatt tgatattcaa gatggtcaac
    34861 gtggaccaca agcaacaaat atcaatcttg ttaaataatt aaaaaagagc ctaaatgaat
    34921 tatttagtct cttttttgtt gcctaaaggc tattttataa taatttcttt aaatcaaata
    34981 tagcattcat agcttgcatt ggagttagat tcattatatc tatcgcttct aactcctcaa
    35041 taaccctacc tgcatttgaa tgttcttcaa aaagcgataa ttgtccttgt tgcttcccag
    35101 gatctagttc ctcatttaag tcagcagtaa cagcttcttg gggggctgat acgatcaact
    35161 gtactgcatc agcttccaac tgtgataaaa tatcagttgc cctatccaat aagtcaattg
    35221 gtaaaccagc tatttttgcg acgtgtatcc cataagactt gtccgcaggt ccaggttcaa
    35281 ttttatgtaa gaaggtaact tctccatctc tctctaaagt agccacgtgt acattgacaa
    35341 gccttgtcaa ttgttcagat aaatctgtca actcatggta atgagttgca aacattgttt
    35401 ttgccctaac acggtcatga atatattcta taatcgattg agctaatgcc ataccatcat
    35461 aagtggctgt ccctcgacct aattcatcaa aaagaatcaa agatttatca ctggctcgtt
    35521 ttacagcttg attcgcttcc atcatttcta ccataaaggt tgattgccca gaaattaagt
    35581 catcagcagc accaatccta gtaaatattg catcaaatac aggcaaatca acttcgtctg
    35641 ccgatacaaa acctcccatt tgtgccataa taactgtcag agctaactgg cgcatatagg
    35701 tcgacttacc actcatattt ggtcctgtaa tcaactggat atctgtctga ctatcaaaat
    35761 agatactatt gggaatatat tcttgcactc ccatcacttt ttcaacggtt gcatgacgcc
    35821 cattcttaat cttaatctga tgttcatcat taaatttggg acgaacatag tgatgatttt
    35881 ctgcaactac tgccaaactc tgtagaacat caacggttgc aatagtcttt gctaactctt
    35941 gaagacgttt aatataagat tctacttgag cacgaacacg cataaaaata tcatattcta
    36001 aatttgaaga ttgctcgcga gcttcgagca tttcaccttc aattttggct agttctgctg
    36061 ttccatagcg ttcagaattt tttaatgtcg ctttacggaa aaaatgctcc ggtactagtg
    36121 ataaattgga attggtcaca tggaagtaat agccgtcttt tttattataa tcaattttaa
    36181 gggtaccgat accacttgct gctctttcct tagcttcaat atcagcaatc caacctgtac
    36241 cttcacgcat cactgttcga taattatcca attgcttatc aaatccagac ttgataatgt
    36301 ttccctcagt gatagtagcc tgtgcttctg gggctatcgc cgtattaatt aaactttcta
    36361 attcaggcat agtgtcaatt ttgttgacaa tgatgtcaag ctcaggttga ttgaaggact
    36421 gtaaaatcat tttaatccga ggaatttgtg ataaggtctg tccgagttgc aatagatctt
    36481 tagggttggc ttttccgaaa gatactcgac ttgctaggcg ttcaatatca tatacaccct
    36541 ttaaactttc tgtgagatcg tttctctcaa aaaaataatc aagaaacact tgaataatat
    36601 cttgacgttc cttgattcga ttcatactta ccaaaggcct gtcaatccaa gttctcagca
    36661 ttcgagttcc catcgctgtt ttagtctcat ctaacaacca gtaaagactt ccgtgcttct
    36721 tgcttgttct agcattctcc agtaaatcta gactattttt cgttgcatat gacatttgta
    36781 aatagtcctt tatttcataa tgaactactt tctgtaaatg actaagttca cgcttttgtg
    36841 ttctatgcac atattgtaaa agtttttccg cagcagatat ttccaaatca gttaactgct
    36901 catcaatcaa atgaatgtca ttaagtcgtt cgtcttcgta tgataaaagc aagttcatct
    36961 gcttcgtcag taggtgattt ttttcgtccg tcaattgata tcctactaca atctcacgag
    37021 cttttaaatt tagtatttca ctacggacgg actcaaaatc tgttaaaagg gttgcctgaa
    37081 actctccagt tgagacatcc atatatgcta gaccaaatgt ttgttgatct tgcgaatcaa
    37141 tcgctactaa gaaattatta gcactatccg gcttcgttga ctccacaacc gttcctgggg
    37201 taacaacttg cactacctca cgcttgacca ctcccacagc ttttttagga tcttccatct
    37261 gctcagcaat ggctactttg taacctaatt caactaaaac gtcaatatac tgttgagctg
    37321 agtgataggg aactcctgcc attgggattg gcttttctgc gttcttattt cgactagtca
    37381 agctaatttc caggatttgt gctgctttta ccgcatcatc ataaaataat tcataaaaat
    37441 cacccattct aaaaagcaaa aaagcatctg gataattctc ttttatatcc agatactgtt
    37501 gcattcccgg tgatatcgtt ggcttggcca tttctcactc ctaactaacg gggctggctg
    37561 ccatgctcag ctcctcaatc attatattta taaacctact gatagttctt tttcaaaaac
    37621 tttggcaata tcttcatttt gacaaatcac caacaaggtg tctgctcctg ccattgttcc
    37681 taaaatctct ggcagccctt tctcatcaat aaagtttgct aaaacgctag cctcccctaa
    37741 attagtattt aaaactaaca taaaacttgc tcgcgatact ttcaaaatat aggagcgtaa
    37801 ggctgggcta aatcgatttg aagtagctgt tgataaacta taatatagct taccttctgg
    37861 gctacgcaat tttaaaagac caatctcacg caagtctctt gatagagtcg cttgcgtgac
    37921 atcgattcct tcatttttta aatgtaattt aatttcttct tgtgtgccaa tctgccctga
    37981 ttggattaaa cgtttaatac gtttttggcg ttctatttta ttcatgttat tcaatccttt
    38041 ttagttagaa ttatgcttac caaattatat caaaaaaaat tgtctttttc acttagtagt
    38101 gatatcgtaa tcattctgta aaaaagctat aaagtaggct agttctttac agaatgcatt
    38161 atgaatttaa tcaacaagtt ctcgtctatt tttcataatt atgttaaaat agggtgacta
    38221 tattactaat taaaatagga gataccatgg atacaaaaca tctaattgcg agtgaaattc
    38281 aaaaagttgt tccatatatg gaacaatcgg ccattctttc tttattagaa accccaaaaa
    38341 attcgagcat gggagattta gctttcccag cattctcttt ggctaaaact ctacgcaaag
    38401 cacctcaaat aattgctagt gacattgctg aacaaattaa aagcgaccaa tttgaaaagg
    38461 tggaagctgt tggaccttac gttaactttt tccttgataa agccgcaatc tcaagtcaag
    38521 ttttaaaaca agtcttatct gatggttctg cctatgctac tcaaaatatt ggtgaaggac
    38581 gaaatgttgc cattgatatg tctagtccaa atattgctaa gccattctca attggacacc
    38641 ttcgttcaac agttattggt gatagtttag ctaatatttt tgataaaatt ggctatcatc
    38701 ctgttaaaat taatcacctt ggcgactggg gtaaacaatt tggaatgtta atcgttgcct
    38761 ataaaaaatg gggcaatgaa gaggctgtcc gtgctcatcc cattgatgaa cttttaaaac
    38821 tttatgtccg tattaatgct gaagccgaga tagaccctag cgttgatgaa gaggcacgtg
    38881 agtggtttcg taaacttgag gctaacgatc ctgaagcaac tgaattatgg caatggttcc
    38941 gtgacgagtc attattagaa ttcaaccgtc tttacgataa aatgaatgtt acatttgata
    39001 gctataacgg cgaggcattc tacaacgaca aaatggatga agtgcttgaa ctgctagaat
    39061 ctaaaaaact cctagtcgaa tctaaaggtg cacaagttgt taacctagaa aaatacggta
    39121 ttgaacatcc tgccctcatt aaaaaatccg atggagcaac tctctacatt acacgtgact
    39181 tagctgctgc actttaccgt aaacgtacct acgattttgc aaaatcaatc tacgttgtag
    39241 gaaacgaaca gtctgcacac tttaaacaac taaaagctgt tcttaaagaa atggactacg
    39301 attggtccga tgatatgact catgttcctt ttggccttgt aacaaaagga ggagcaaaat
    39361 tatcaactcg taaaggaaat gtaattctcc ttgaacctac tgttgcagaa gctattaatc
    39421 gtgcggcatc tcagattgaa gctaaaaacc ctaacctagc agataaagat aaagttgctc
    39481 aagcagttgg tgtaggtgct attaaatttt acgatttgaa aactgatcgt acaaatggtt
    39541 atgactttga cttagaagca atggtatcat ttgaaggtga aactgggcca tatgtacaat
    39601 atgcacatgc tcgtattcaa tcaatcttac gtaaagctaa ctttaaccca tctaatagcg
    39661 ataattacag tctcaacgat gttgaaagct gggaaatcat caaactcatc caagattttc
    39721 ctcgtattat tgtgcgtgca gcagataatt ttgagccctc tattattgca aaattcgcta
    39781 tcaatttagc tcagtgcttc aataaatatt acgcccacac acgtatttta gatgaagatg
    39841 ctgaaatcag tagtcgtcta gcactttgct atgcaactgc tacagtatta aaagaatctc
    39901 ttcgcctcct aggagttgat gctccgaatg aaatgtaaga aatgacatta ctaagactgt
    39961 caaatttatg acagtcttaa ttttttatat tataataata gcacaataag ttacaggaga
    40021 atgccatgcc atcagaaaaa gaaatattag atgctcttag taaagtttat agcgaagaag
    40081 taatacaagc agatgattat tttcgtcaag ctattttcga gctcgctagt caacttgaaa
    40141 aagaggggat gaactcactc ctagctacaa aaattgactc acttatcaat caatacgtct
    40201 taacacatca atttgatgct cctaaaagca tatttgatct tagtcgcttg gtaaaaacaa
    40261 aagctagcca ttacaaggga actgctatct cagctattat gcttggtagt ttcttaagtg
    40321 gaggaccaaa ataaaaatac aggccagaca gcctgtattt ttatttatcg taatttcttc
    40381 gtcgaatttt aaaatctgag ctaataactt catctacatt tataacagaa ataaaagcat
    40441 ctgggtctaa atcagccatg atttctttca catcacgcac ttcacttggg ttaagagcta
    40501 cgtacaagac atcatgctct ctacctgagt aggcaccttg gccccgtaaa aaagttacac
    40561 ccctattaat ttcatgcaaa atggttgctg ctgcagattc tgattctttg gtaataatga
    40621 tcattccacg aacagtgtag cccccatttt caacatttgt taacacttga ctaaatacaa
    40681 agctagctac taacgtatat agcatctgtt gtaaattaat ataaacaagt gaagacgtta
    40741 gaactatagc atcaataaac aataaggttt gccctaattt tattccagat ttttcctcta
    40801 caatacgacc aataatatct gcaccacctg tagttgcacc atatctaaat actaaaccac
    40861 taccagttcc tgctaaaatt ccagcagcta ctgcaactaa catcatatcg tttttcaaag
    40921 ggactgttac agggatttgt tgccaaaacc acatgaaaaa agacattaac actgtaccat
    40981 atatagttaa taataaggat tttttcccta aaatcctagc ccctaatata aataacggga
    41041 tattaaggag aagtgatgaa agtgctggat taactccgaa aagtgcatgg ataatcaaag
    41101 tgacaccgga aatacctcct tcagccaaat gattagccat attaaatttg acaaagccga
    41161 aagtatagag agccacacca aaggcaatta aaatagcttc ttttatcttt gtttttaaat
    41221 ccagtttcaa cataggctta atccttcctt attcaaaaaa gctaaagttg tttaaacaac
    41281 ttagcttaat aacatcataa tgtttcctta gtggcggttg accaaaagtc atatatacag
    41341 ttaatcatca tcaacgaatc gtccaataat atgaacattt tctgcaacgg aaacaaaggc
    41401 ttttggatct gcttttagca ttaaatattt aaaatcacta aattcttcac gtgttaatat
    41461 agttattaaa actgcttttt tctcatgatt ataagtacct tcagcatcat tgatacaggt
    41521 tacaccacga tgtaaatcac gatggatacg tttaatgacg caatcaggct tactggtaac
    41581 aatcatcgct tgcatctttt tttgctttgt aaaaatggca tctgtaactc tacttgatac
    41641 aaaaattgtc accatcgagt acaaagcgta cttccaacca aataaaagac ctgcaaataa
    41701 taaaataata ccattaataa taaaagaaat actacctaca tctctgcctg ttttctttct
    41761 aattgtcaaa ctgataatat ctgttccccc actagaaatt ctagacttaa agctaaaacc
    41821 aacaccagct cccataatta agccaccaaa gatagcattg attaagggat cagttgtaag
    41881 ggtgatttga ggcattagtt gaatgaagat agagcttaca gttactgtga taaacgtaaa
    41941 aatagtaaat ttatgtccaa tcttacgcca agataatatc agcaaaggga tattaatggc
    42001 ataaaaagct aaagcaactg gtatttcaaa agaaaaccag tgtttactga cagctgaaat
    42061 aacctgagct aaaccagttg ctccacttga ataaacatgt ccaggttgaa aaaagaaatt
    42121 aacagcaaca ctggatagaa taccatacag taatgaagcc gaaattttct ctgcatattt
    42181 ttctcgtgag atacttttta aagtgtgtaa gagaccaaat tttttagccc atacactgat
    42241 aatatatttt accttttttt ctaaaggggt tcgtctagtt ttcataactt tctacagtaa
    42301 gtgataattc ttctaattgt tgttctgaaa cgagactagg agcttgagtc attggatcac
    42361 tagccttatt gtttttaggg aatgcaataa cttctcgaat attgtccttg cctgctaaca
    42421 acataacaaa gcggtcaagg cctattgcca atccaccatg tggtgggaag ccgtaatcca
    42481 tagcttcaag taagaaacca aactgttcct gagctgattc agcgctaaat cctaaagcct
    42541 tgaacatacg ctcttgtgta tctttttggt taatacgtag gctaccaccg cctaactcat
    42601 atccattcaa aacaatatca taggcaactg cacgtacttt agccaaatcc ccttccaact
    42661 catgcgctgt ctccgctgtt ggcaaggtga atggatgatg agccgacata tatcgtccct
    42721 cttcttctga ccactcaaac attggccaat caacaaccca taggaaatta aatttagaat
    42781 aatcaatgag ttcaagttct tttgcaatgc gtgtacgaag cgctcctaaa gtctcgttag
    42841 ctacttcaag actatctgct acaaatagaa ttaagtcatt attctctagc tgcaaagctt
    42901 ctgtcaacct accttcaata gctgtcaaga acttagcaac cggacctgaa atagtattat
    42961 ctacatattt tagccatgct aaacctttgg caccgtattg ttttgcaatt tctgttaatt
    43021 tatcaatatt ttttcgagag tacttgtccg ccttgtcttt gacaacaatc gctttaacta
    43081 ctgaagcttc tgagaacacc ttgaagtcaa cttcttttac aatttctgtc aagtcttgta
    43141 aaagcatatc aaaccttgta tcaggcttat cagacccgta gttattcatt gcatcatcat
    43201 atgccatcct tgggaatggt aaagaaacct ctaaaccttt agtgtctttc attactttag
    43261 caatcatacc ctcaacaatg tcttgaattt cttgatcact aaggaatgat gtctctaagt
    43321 caacttgtgt aaactcaggt tgacgatctc cacgtaaatc ctcatcacga aaacatttga
    43381 caatttgata ataacgatca aatcctgcat tcatcaatag ctgttttgta atttgagggc
    43441 tttgtggaag ggcataaaaa tgtccttggt tgacacgtga tggaaccaaa tagtctcgtg
    43501 ccccctctgg agttgatttt gttagcatcg gagtttcgac atcaataaac tctaagttgt
    43561 caaggtaatt acggattgag tgtgttactt tcgcacgtaa tttgaaattt tcaagcattt
    43621 ccggacgacg aagatccaag taacggtaac gcatgcgtgt atcatcattt gcctcaatac
    43681 catctttaat ttcaaatggt gttgttttcg aggtattaag aaccgataat tcttgcactt
    43741 ttaattcaac ttctcctgtt ggtaaattat cattagcttg ttctcgtgct gtcactacac
    43801 cgctaacctc tataacaaat tcactacgga gactttctgc ggttgccata actgaggctg
    43861 ctacttcttc aggattaata actaattgca tgattccctc gcgatcgcga agatcaataa
    43921 aaataaggcc tccaaggtca cgacgacgac caacccatcc ttttaaagta atcgatgttc
    43981 ctatgtgctc actacgaaca cgcccagcat acatactacg tttcatctat tttctccatt
    44041 taaaatattc attccaacct attataacaa aaagcaccta aaaaccccac ccattaagga
    44101 gagtttttag gaactgtttt acaatcctaa ttgtttaagc acagatgaaa aatctgtttt
    44161 aatgttttca aaactaactt taacttcttg acgtgtttga ttatttttta aaccgacctc
    44221 tttcgaatct acttcactgc tacctaatgt cataatcact ttagcattaa aagtatctgc
    44281 agacttaaat tgtgctttga ttttacgacc caaataatca cgctctactt taaaaccttg
    44341 atgacgtatt gattgtgcca aatctaaggc agctagatta gcctctgatc ctaatacagc
    44401 tatatacaga tctatagtat tttctattgg taaagaaatc ccttgcttat ctaatattaa
    44461 gagcaagcgt tctaatccta atccaaaacc aaatgctggt gtttctggtc caccaaaata
    44521 ggatactaag ccgtcataac ggcctccagc acagatcgtt aattcattat cttcaatctc
    44581 agtgataaac tcaaaaatag tatggttata ataatcaaga ccacgaacca tattagtatc
    44641 aatgatgtat ggaatattta gagcatcaag catatgacac acagcatcaa agtgggcttg
    44701 gcttgactca tctaaataat ctaaaataga aggagcattt tcaactgcta gtttgtcttc
    44761 tggctcctta gagtctagta cgcgcaatgg attttcattc aaacgacgtt gactatcctt
    44821 agacaattgt tcacgtatgg gtgttaagta atcaataaga gcttggcgat aggcttgacg
    44881 actttctgga cttcctaaac tattaagatg aagtttaacg tttttgatac ctaaatcttc
    44941 aaatagatga tgtcccattg cgattgtttc aacatcagtt gttggattat ttgatccaaa
    45001 gcactcaacg cctacttgat gaaattctcg taaacgacca gcctgtgggc gctcatatcg
    45061 gaacattgat ccaatataat acattttggt tggtttttga acttctggtg caaatagttt
    45121 attttcaaca taagaacgta caacaggtgc tgttccttct ggacgaagcg taatgtgacg
    45181 atctccttta tcatgaaaat catacatctc ttttgtaacg atatccgttg tatccccaac
    45241 tgagcgacta ataacctcgt aatgttcaaa cattggtgta cgaatttcat cataatgata
    45301 ttgtttaaag agattgcgta taacattttc aacgtattgc cactttgctg attctccagg
    45361 taagatatcc tgagtccctt ttggtttttg aagtttcata agtttacctc ttcgattagt
    45421 tttctcttta aacattctac cattttttct tatattttaa acaagcatat atcatttgct
    45481 ttagtttgat agtattttca ccatgaaatt gtaaaaataa aaagttttgt caagtaactt
    45541 tactttacaa aaaagatatg ttatcatagt gaagttgatg aaaatcaaaa cttacacatc
    45601 gaaaggaaga ataagaaaat ggcagtacct gcacgtcaca cttcaaaagc gaagaaaaac
    45661 aaacgtcgta cacactacaa attgacagct ccatctgtac aattcgacga aactactgga
    45721 gattactcac gttcacaccg tgtatcactc aaaggatact acaaaggacg taaaatcgct
    45781 aaagctaacg aagctaaata atagaaggga gataccatgc gcgtaaatat tacacttgaa
    45841 cataaagaat ctggtgaacg cttgtacctt acttcaaaaa acaaacgtaa cactccagac
    45901 cgtcttcaat tgaaaaaata ctcaccaaaa cttcgtaaac acgttgtttt cactgaagtt
    45961 aagtaagggt ttcagtacac gtcaatagac gtcaaaatat tgattttaag cgatttttga
    46021 ttttttgaaa ctcattatat cgcactacaa atcaataaaa actctacctt ttactctacc
    46081 ttttttagat aaatatatca tctggatatt gaagttaagc cctgctatca tttcgatggc
    46141 agggcttttt aatatttgta cactaacttc ttatctatat tttggtggat tttaagttga
    46201 catctacaaa gtttaatgtt agaatacatt caaaaatata tttgaatgag gtattttatg
    46261 attcaaaatg ttgttacttc aataatcctg tattctggga cagccgtaga cttacttatt
    46321 atcctaatgt tattttttgc caaaagaaaa agcagaaaag acatcattaa catctattta
    46381 ggacaatttc taggctctgt tagtctaata ttcctaagtt tgctttttgc atttgtctta
    46441 aattatattc ctagtaaaga gattttaggt ttactcggtt tgattccaat tttcctaggc
    46501 ctaaaagttt tgcttttagg agattctgat ggagaagcta ttgcaaaaga tggtttgcga
    46561 aaagacaata aaaacctgat ttttctagtc gctatgatta cttttgcaag ttgtggcgct
    46621 gacaatattg gtgtctttgt cccatatttt accaccttaa atttagcgaa tttgatagtg
    46681 actttactta cttttctagt catgatttat ctcttggttt tttctgcaca aaaattagca
    46741 caagtccctt ctgttggaga aactttggaa aaatatagca gatggtttat tgccgttgtc
    46801 tatttaggat tggggatgta tatcctgatt gaaaacaaca gctttgacat gctatgggct
    46861 gtgttaggct aggagaaaaa attatgaaaa aagatagtat ctgtcaagtg gatgttataa
    46921 atcaacaaaa tgttacaacc gcaacgaact accttgaaaa ggaaaaagtc caaaaatcac
    46981 ttcgcatttt atcaaaattt accgataata aacagataaa tatcatcttt tatctccttg
    47041 ccgtcgaaga actctgtgtc tgcgatatag cctgtttatt aaatctcagt atggcatctg
    47101 cctcccacca tcttcgtaaa ctagccaatc aaaacatctt ggacactaga agagagggga
    47161 aaattatata ttattttata aaagatgagg aaatcagaga tttttttaat caactaggat
    47221 aacaactatt tctactactt t
//
LOCUS       DATROD010000006        44661 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.006, whole genome shotgun sequence.
ACCESSION   DATROD010000006 DATROD010000000
VERSION     DATROD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44661
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.006"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..184)
                     /locus_tag="VBC74_000327"
     CDS             complement(<1..184)
                     /locus_tag="VBC74_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEO6941306.1"
                     /translation="MTSSLITKKKIAKSFKRLFISQAFDKISVSDIMEDAGIRRQTFY
                     NHFVDKYALLEWIFQTE"
     gene            358..1347
                     /gene="dhaK"
                     /locus_tag="VBC74_000328"
     CDS             358..1347
                     /gene="dhaK"
                     /locus_tag="VBC74_000328"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEO6941307.1"
                     /translation="MKKILNQPTDVVTEMLDGLAYVHNDLVHRIEGFDIIARNEEKSG
                     KVALISGGGSGHEPSHAGFVGEGMLSAAVCGAVFTSPTPDQVLEAIKEADEGAGVFMV
                     IKNYSGDIMNFEMAQDMAEMEGIEVASVVVDDDIAVEDSLYTQGKRGVAGTILVHKIL
                     GHAARHGKSLQEIKAIADELVPNIHTVGLALSGATVPEVGKPGFVLAEDEIEFGIGIH
                     GEPGYRKEKMQPSKALATELVDKLIESFDAKSGEKYGVLINGMGATPLMEQYVFANDV
                     AKLLEDKGIEVNYKKLGNYMTSIDMAGLSLTLIKLENQEWLEALNSDVTTIAW"
     gene            1385..1963
                     /gene="dhaL"
                     /locus_tag="VBC74_000329"
     CDS             1385..1963
                     /gene="dhaL"
                     /locus_tag="VBC74_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEO6941308.1"
                     /translation="MEVKTAIEWMHTFNQKIQSNKDYLSELDTPIGDGDHGGNMARGM
                     TAVIENLDNNEFSSAADVFKTVSMQLLSKVGGASGPLYGSAFMGITKAEQSESTISEA
                     LGAGLEMIQKRGKAELNEKTMVDVWHGVIEAIEKNELTEDRIDSLVDATKGMKATKGR
                     ASYVGERSVGHIDPGSFSSGLLFKALLEVGGV"
     gene            1963..2337
                     /locus_tag="VBC74_000330"
     CDS             1963..2337
                     /locus_tag="VBC74_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEO6941309.1"
                     /translation="MSDIGIIVVSHSKNIAQGVVDLISEVAKDVSITYVGGTEDGEIG
                     TSFDQVQQIVEQNDKKTLLAFFDLGSAKMNLELVADFSEKNIIINSVPVVEGAYTAAA
                     LLQAGADLDSIQSQLAELTINK"
     gene            2347..3060
                     /locus_tag="VBC74_000331"
     CDS             2347..3060
                     /locus_tag="VBC74_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO6941310.1"
                     /translation="MTQFLGEFLGTFILVLLGDGVVAGNVLSKTKEEGTGWTAIVFGW
                     GIACTVAVYVSGLFSPAHLNPAVTLAMASIGVISWGQVIPFIIAQMLGAMVAATILWL
                     HYYPHWKETKDSGLILASFSTGPAIRHTPSNLLGEIIGTAILVITIMAIGPSKVAAGL
                     DPIIVGIVIFAVGFSLGPTTGYAINPARDLGPRLMHAILPIENKGNSDWSYAWIPVVG
                     PIIGGVLGAILYNIALTLV"
     gene            complement(3325..3729)
                     /locus_tag="VBC74_000332"
     CDS             complement(3325..3729)
                     /locus_tag="VBC74_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO6941311.1"
                     /translation="MKGFHGNIEKLTLGNTNFRQVLYTAEHCQLVLMTLPVGGEIGSE
                     IHAENDQFFRFEAGHGKVVIDGNEYEVADGDAIIVPAGAEHNVINTSETEMLKLYTIY
                     SPAHHKDGIIRATREEAEENEEDFDGVTTETK"
     gene            complement(3868..4582)
                     /locus_tag="VBC74_000333"
                     /pseudo
     CDS             complement(3868..4582)
                     /locus_tag="VBC74_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859560.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(4675..5784)
                     /locus_tag="VBC74_000334"
     CDS             complement(4675..5784)
                     /locus_tag="VBC74_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEO6941312.1"
                     /translation="MTVTGIVAEFNPFHNGHKYLLEQAQGIKVIAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVIELPFLVSVQSADYFASGAVSILARLGVDNLCFGTEEMLD
                     YARIGDIYVNKKEEMEAFLKKQSDSLSYPQKMQAMWQEFAGITFSGQTPNHILGLAYT
                     KAASQNGIRLNPIQRQGAGYHSSEKTEIFASATSLRKHQSDRFFVEKGMPNSDLFLNS
                     PQVVWQDYFSLLKYQIMTHSDLTQIYQVNEEIANRIKSQIRYVETVDELVDKVATKRY
                     TKARIRRLLTYILINAVESPIPNAIHVLGFTQKGQQHLKSVKKSVDIVTRIGSQTWDS
                     LTQRADSVYQMGNANIAEQTWGRIPFYQPVSQSDL"
     gene            complement(5781..6032)
                     /locus_tag="VBC74_000335"
     CDS             complement(5781..6032)
                     /locus_tag="VBC74_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941313.1"
                     /translation="MIIFLYACLMIFGILYIIERRVSQKKSKVILELLMNPKLHLDLL
                     SALKPFEKETDRLIYLRKTYHLDIIDAVTLNNQLKEKEV"
     gene            complement(6055..6789)
                     /locus_tag="VBC74_000336"
     CDS             complement(6055..6789)
                     /locus_tag="VBC74_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6941314.1"
                     /translation="MATYETFAAVYDAVMDDTLYDKWTDFSLRHFPKGKKKLLELACG
                     TGIQSVRFAQAGYAVTGLDLSGDMLKLAKKRATSAHQSIQFIEGNMLDLSNVGKYDLI
                     TCYSDSICYMQDEVEVGDVFIEVYKALEENGVFIFDVHSTYQTDKVFPGYSYHENADD
                     FAMVWDTYEDDAPHSIVHELTFFVQEEDGRFTRHDEVHEERTYDILTYDILLEQAGFK
                     DVKVYADFEDKKPTATSARWFFVAHK"
     gene            complement(6855..7211)
                     /gene="rsfS"
                     /locus_tag="VBC74_000337"
     CDS             complement(6855..7211)
                     /gene="rsfS"
                     /locus_tag="VBC74_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEO6941315.1"
                     /translation="MTEKDLLQLVVKAADEKRAEDIVILDLQPVTSVADYFVIMSASN
                     SRQLEAIADNIREQVKGNGGDASHLEGDSKAGWVLLDLNSVVVHIFSEDERQHYNLEK
                     LWHEAPLLDAEVFMTE"
     gene            complement(7213..7734)
                     /locus_tag="VBC74_000338"
     CDS             complement(7213..7734)
                     /locus_tag="VBC74_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO6941316.1"
                     /translation="MTCALLLIDIQQGIMDKKPKHLTNFAELLDDLLLSAKGSNCEVI
                     WIRHHDKELPQGSPQWEIWEQRHLVTHHKIIDKTYNSCFKDTHLHDYLQSKHISQLIM
                     MGLQTEYCFDTSVKVAFEYGYDIFIPQGGHLTFDTPTLSGDSIKKHYENIWHHRFATM
                     VAKDSLLKLINEE"
     gene            complement(7797..8384)
                     /gene="yqeK"
                     /locus_tag="VBC74_000339"
     CDS             complement(7797..8384)
                     /gene="yqeK"
                     /locus_tag="VBC74_000339"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEO6941317.1"
                     /translation="MTYKDYTGLDRTELLSKVRHMMSDKRFNHVLGVERAAIELAERY
                     GYDKEKAGLAALLHDYAKELSDDEFLRLIDKYQLDPDLKKWGNNIWHGLVGIYKIQED
                     LAIKDQDILAAIAKHTVGSAQMSTLDKIVYVADYIEHNRDFPGVEEARELAKVDLNKA
                     VAYETARTVAFLASKAQPIYPKTIETYNAYIPYLD"
     gene            complement(8381..9013)
                     /locus_tag="VBC74_000340"
     CDS             complement(8381..9013)
                     /locus_tag="VBC74_000340"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEO6941318.1"
                     /translation="MALELLTPFTKVELEEKKRDTNRKQIGIMGGNFNPVHNAHLVVA
                     DQVRQQLCLDQVLLMPEFQPPHIDKKETIDEQHRLKMLELAIEGIDGLSIEPIEIERK
                     CISYTYDTMKLLIEKNPDVDYYFIIGADMVEYLPKWHRIDELVKMVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRQFIKSNRQPNYLLPREVLDYIRKEGLYK"
     gene            complement(9143..9460)
                     /gene="yhbY"
                     /locus_tag="VBC74_000341"
     CDS             complement(9143..9460)
                     /gene="yhbY"
                     /locus_tag="VBC74_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEO6941319.1"
                     /translation="MLTSKQRAFLKSEAHSMKPIIQIGKNGLNDQIKTSVRNALDARE
                     LIKVTLLQNTDEDIHDVAEVLEDEIGCDTVLKIGRILILYKESARKENRKISVKVKAV
                     KSN"
     gene            complement(9553..10671)
                     /gene="yqeH"
                     /locus_tag="VBC74_000342"
     CDS             complement(9553..10671)
                     /gene="yqeH"
                     /locus_tag="VBC74_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEO6941320.1"
                     /translation="MEELFCIGCGARIQTENKDAAGYTPRAALEKGLETGELYCQRCF
                     RLRHYNEITDVHITDDEFLKLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFVAGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPVDVILTSAQNHHAIKDLIDTIEKY
                     RHGRDVYVVGVTNVGKSTLINAIIREITGSRDVITTSRFPGTTLDKIEIPLDDGSYIF
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNSEQTLFLAGLARFDFISGQKQ
                     GFTAYFDNNLNLHRTKLVGADEFYTKHVGKLLTPPTGKEVSDFPKLVRHEFTIKDKMD
                     IVYSGLGWIRVKSEAENPVVVAAWAPEGVAVVLRKALI"
     gene            complement(10671..11231)
                     /locus_tag="VBC74_000343"
     CDS             complement(10671..11231)
                     /locus_tag="VBC74_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEO6941321.1"
                     /translation="MNSKIYFLEMKLSIDDYRPTFVVEAVYDLRAEDLLRHGIRAVLV
                     DLDNTLIAWNNPDGTAEVRAWLDEMTTADISVVVVSNNNHARVERAVSRFGVDFVSRA
                     MKPFTRGINMAIERYGFDRDEVIMVGDQLMTDIRASYRAGIKSVLVKPIVKSDAWNTK
                     FNRLRERRVWKKIEENYGKIVYQKGI"
     gene            complement(11315..12229)
                     /locus_tag="VBC74_000344"
     CDS             complement(11315..12229)
                     /locus_tag="VBC74_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEO6941322.1"
                     /translation="MTKKEKGTMMTLAAGLAWGISGISGQYLMSHGVHVNLLTSLRLL
                     ITGIFLLSLARSKQKEHLVAAWKQPKFLKQVLLFSIFGLVLNQYAFLRAIHLTNAGTA
                     TVLQYMAPILILSIVCILNRQRPTSFEIIAIAMAILGTYMIATHGKLGSLAITPKGLM
                     WGLGSAITYSIYILLPVKLIHEWGSTIVIGSGMFIGGILFSLVTKAWQYPLQINVMSI
                     LAYIGIIGIGTIFAYTFFLKGVSIVGAVKGSLLASVEPVSSVFLTVLVLGEIFYPIDL
                     LGMLFIFLAVTLISYKDLIALKKMKRVR"
     gene            complement(12359..13801)
                     /gene="gatB"
                     /locus_tag="VBC74_000345"
     CDS             complement(12359..13801)
                     /gene="gatB"
                     /locus_tag="VBC74_000345"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEO6941323.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPSSAHFGQEQNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHQNMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIELEDGTRKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEEFGTKAELKNLNS
                     FNNVRKGLIHEEKRQAQVLRSGGQIQQETRRFDETTGETILMRVKEGSSDYRYFPEPD
                     LPLFDISDEWIDQVRLELPEFPQERRAKYVSSFGLSSYDASQLTATKATSDFFEKAVA
                     IGGDAKQVSNWLQGEVAQFLNSESKSIEEIGLTPENLVEMIGLIADGTISSKIAKKVF
                     VHLAKNGGSAEEFVKKAGLVQISDPEVLIPIIHQVFADNEAAVIDFKSGKRNADKAFT
                     GYLMKATKGQANPQVALKLLAQELAKLKEE"
     gene            complement(13801..15267)
                     /gene="gatA"
                     /locus_tag="VBC74_000346"
     CDS             complement(13801..15267)
                     /gene="gatA"
                     /locus_tag="VBC74_000346"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEO6941324.1"
                     /translation="MSFNNQSIDQLHDFLVKKEISATELTKATLEDIHAREQAVGSFI
                     TISDEMAIAQAKEIDDKGIDADNVMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATAVEKLYAKDMIVIGKANMDEFAMGGSTETSYFKKTNNAWDHSKVPGGSSGGSAAAV
                     ASGQVRLSLGSDTGGSIRQPASFNGIVGMKPTYGRVSRFGLFAFGSSLDQIGPMSQTV
                     KENAQLLTVISGHDVRDSTSSERTVGDFTAKIGQDIQGMKIALPKEYLGEGIAQGVKE
                     TIIKAAKHLEKLGAVIEEVSLPHSKYGVAVYYIVASSEASSNLQRFDGIRYGYRTENY
                     KNLDDIYVNTRSEGFGDEVKRRIMLGTFSLSSGYYDAYYKKAGQVRSLIIQDFEKVFA
                     DYDLILGPTAPTTAFDLDSLNHDPVAMYLADILTIPVNLAGLPGISIPAGFDQGLPVG
                     MQLIGPKFSEETIYQVAAAFEATTDYHKQQPKIFGGEN"
     gene            complement(15267..15569)
                     /gene="gatC"
                     /locus_tag="VBC74_000347"
     CDS             complement(15267..15569)
                     /gene="gatC"
                     /locus_tag="VBC74_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEO6941325.1"
                     /translation="MKISEEEVRHVAHLSKLRFSDQETKEFASSLSKIVDMIELLNEV
                     DTEGVPVTTTMADRKTVMREDIAQPGHNRDDLFKNVPQHQDYYIKVPAILEDGGDA"
     gene            complement(15707..18352)
                     /locus_tag="VBC74_000348"
     CDS             complement(15707..18352)
                     /locus_tag="VBC74_000348"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEO6941326.1"
                     /translation="METKFVYHFDEGCKEMKELLGGKGANLAEMTSIGLPVPQGFTIT
                     TQACNDYYDNACHIRESILSQIDQAMAQLEVEQNKQLGSVDDPLLVSVRSGSVFSMPG
                     MMDTVLNLGLNDRSVQGLVKKTEDERFAYDSYRRFIQMFADVVTGIPKYKFDTILDRL
                     KTDKCYQDDTELTGSDLKRLVEFYKELYQKEAGEKFPQDPKRQLLLAIEAVFKSWNNP
                     RAKIYRKLNDIPETLGTAVNIQAMVFGNMGNNSGTGVAFTRNPSTGAANLFGEYLINA
                     QGEDVVAGIRTPQSISKLAEQMPIIYQEFVSVTQKLEAHYRDMQDMEFTIENGNLYML
                     QTRSGKRTAKAAIKIAVDQVNEGLISKEEAILRIEPKQLDQLLHPSFDLKSLKKAIIL
                     TTGLPASPGAAYGKVYFHAEDVVKEMKKGNPVLLVRQETSPEDIEGMVSANGIITARG
                     GMTSHAAVVARGMGKPCVAGCSQLLVDEVRREISIGHQTIKEGEMLSIDGATGNVYIG
                     QVPMAETSVDRDFEIFMKWVDENRDMMVCSNADNPRDAQKALDFGAEGIGLCRTEHMF
                     FDDERIPVVREMILADEILSRRKALERLLSFQRDDFYQIFKVLKGKACTIRLLDPPLH
                     EFLPHDKESIESMARQMGISTLAIEKRIQTLEEFNPMLGHRGCRLAITYPEIYQMQVR
                     ALVQGAILAMKEGYEAKPEIMIPLVTAHEEISIIRDLIEETIVEESKSKKINLSFPIG
                     TMIETPRACMIADDIAKFADFFSFGTNDLTQMSFGFSRDDAGKFLGEYVDKGLLKKDP
                     FQVLDQKGIGRFIEQAVRLGKEVKPNLKIGICGEHGGEPSSIEFCYQQGLHYVSCSPF
                     RIPIAKLAAAQAKIKQSRNDITIDK"
     gene            complement(18365..19195)
                     /locus_tag="VBC74_000349"
     CDS             complement(18365..19195)
                     /locus_tag="VBC74_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEO6941327.1"
                     /translation="MEDQLTIFIISDSLGETAKAIAKACLSQFPGHDDWHFQRFSYIN
                     SQERLEQVFEEASQKTVFMMFSLVDVALASYAQKRCESEHYAYVDLLTNVIQGISRIS
                     GIDPLGEPGILRRLDNDYFKRVESIEFAVKYDDGRDPRGILQADLVIIGISRTSKTPL
                     SMFLADKNIKVINIPLVPEVPVPKELRMIDSRRIIGLTNSVDHLNQVRKVRLKSLGLS
                     STANYASLERILEETRYAEEVMKNLGCPIINVSDKAIEETATIILEILKTNGQVAKNL
                     "
     gene            complement(19206..19856)
                     /locus_tag="VBC74_000350"
     CDS             complement(19206..19856)
                     /locus_tag="VBC74_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6941328.1"
                     /translation="MYKEGDLIQLNQRQERIIDIIKQFQPITGELIAEKLGVTRAALR
                     SDLAVLSMLGIIDAKPKVGYFYLGQYHASIGTSHFEKMTVSEIMGIPLTVHQKDSVYD
                     VIVHIFMEDAGCAFILDDDDFLCGVVSRKDLLKTSIGGGDLSKMPIGMVMTRMPHVTT
                     VLENESLFAAADKLVSRKVDSLPVVRHDKQYPEKFKVIGKLSKTILASLFLEIRDY"
     gene            20024..20989
                     /locus_tag="VBC74_000351"
     CDS             20024..20989
                     /locus_tag="VBC74_000351"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEO6941329.1"
                     /translation="MTIKNLTVAGSGVLGSQIAFQAAYKGMSVTIYDINDEALNKGKE
                     RIKKLAKVYQSEIETAKEAYSDKAKSIKYNKNLLPSLDHIFLSKVADSLDLIADLPNQ
                     ITFSKNLDQAVSDADLVIEAVPETVSIKEDFYKQLAKVAPSKTIFATNSSTLVPSQFA
                     DITGRPDKFLAMHFANNIWQNNIVEIMGHKGTDDEVVKEALAFSKDIGMVPLYIHKEQ
                     PGYILNSILVPFLESALALYYNKVSDSETIDKTWKLGTGAPMGPLEILDIIGIDTAYN
                     IMKNYSDTTSDPNSLHAHLAKMLKEEFIDKGRTGKAAGHGFYDYD"
     gene            complement(21036..21584)
                     /locus_tag="VBC74_000352"
     CDS             complement(21036..21584)
                     /locus_tag="VBC74_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEO6941330.1"
                     /translation="MTKALISIDYTYDFVADDGKLTAGKPAQSIASAIADVTEKAYRS
                     GDYIFFAIDNHDIGDVFHPETNLFPEHNIKGTSGRNLYGPLGTLYETIKEDSRVFWID
                     KRHYSAFSGTDLDIRLRERRVDTLILTGVLTDICVLHTAIDAYNLGYKIEVPAAAVAS
                     LNDSNHQWALNHFKTVLGATIL"
     gene            complement(21651..22436)
                     /gene="codY"
                     /locus_tag="VBC74_000353"
     CDS             complement(21651..22436)
                     /gene="codY"
                     /locus_tag="VBC74_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEO6941331.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLDAELPYNTMAAQLADIIDCNAC
                     IINGGGNLLGYAMKYKTNTDRVEEFFETKQFPDYYVKSASRVYDTEANLSVDNDLSIF
                     PVETKENFQDGITTIAPIYGGGMRLGTFIIWRNDKEFSDDDLILVEIASTVVGIQLLN
                     LQTENLEENIRKQTAVTMAINTLSYSEMKAVAAILGELDGLEGRLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFDKLKEYN"
     gene            complement(22562..23773)
                     /locus_tag="VBC74_000354"
     CDS             complement(22562..23773)
                     /locus_tag="VBC74_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6941332.1"
                     /translation="MKIFDKSMKLEHVAYDIRGPVLEEADRMRANGEKILRLNTGNPA
                     AFGFEAPDEVIRDLITNARESEGYSDSKGIFSARKAVMQYYQLQNIHVDMDDIYIVNG
                     VSEGISMSMQALLDNDDEVLVPMPDYPLWTACVSLAGGNAVHYICDEEANWYPDIDDI
                     KSKITSKTKAIVLINPNNPTGAVYPREILQEIVDIARQNDLIIFSDEVYDRLVMDGME
                     HIPIASIAEDIFTVTLSGLSKSHRICGFRVGWMVLSGPRQHVKGYIEGLNMLANMRLC
                     SNVLAQQVIQTSLGGQQSIDSMLLPGGRIYEQRNYIHKAINEIPGLSAVKPNAGLYLF
                     PKIDTDMYRIDNDEEFVLNFLKQEKVLLTHGRGFNMNTADHFRIVYLPRVDELTELQE
                     KMARFLSQYKR"
     gene            24040..24492
                     /locus_tag="VBC74_000355"
     CDS             24040..24492
                     /locus_tag="VBC74_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO6941333.1"
                     /translation="MSQKYERILVAIDGSTESELAFEKAVNVALRNDSELILTHVIDT
                     RALQSFATFDTYIYEKLEKEAKDVLEEYEKQAREKGADKVRQVIEFGNPKTLLAHDIP
                     EKEKVDLIMVGATGLNTFERFLIGSSSEYILRHAKVDLLIVRDPNKTM"
     gene            complement(24547..25929)
                     /locus_tag="VBC74_000356"
     CDS             complement(24547..25929)
                     /locus_tag="VBC74_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO6941334.1"
                     /translation="MAIKAVFFDIDGTLLNDRKNVQKSTIKAIRNLKDQGILVGLATE
                     RGPSFVQPFLENLGLDFAVTYNGQYIYSRSEIIYTNQLSKTTVYRLIRYAGARRREIS
                     LGTASGLLGSGIIGLGTSRLGQIVSSLVPRKWAKAIERSFKHFIRRIKPQNIDSLMVI
                     LREPIYQVVLVATEGESERIQKQFPRVKLTRSSPYSMDVISEGQSKVKGIERVGQCYG
                     FDLSEVIAFGDSDNDIEMLSQVGIGVAMGNASQQVKENARYTTADNNDDGISKALAHY
                     GLIQFEIEKTFSSRDENFNKVKSFHLLMDGETIKTPRLYDSKEAGFRSDFKVEEIVEF
                     LYAASQGNQEVFDQSIRNLHLAIDKARDKVISKDHPETPLVGEVDALTDLLYLTYGSF
                     VLMGVDPKPLFDTVHEANMGKIFPDGKAHFDPVTHKILKPDDWEEHFAPEPSIRRELD
                     SQIQKSLNRK"
     gene            25998..26960
                     /locus_tag="VBC74_000357"
     CDS             25998..26960
                     /locus_tag="VBC74_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEO6941335.1"
                     /translation="MKKILVLHTGGTISMNANEKGQVMSSADNPMKYVDLSLDDLDLT
                     VVDFLNLPSPQITPHHMLDIYHYLKQHASNFDGVVITHGTDTLEETAYFLDTMILPKI
                     PIIITGAMRSTNELGSDGVYNYLSALRVANSTKAADKGVLVVMNDEIHAAKYVTKTHT
                     TNVSTFQTPTHGPLGIIMKQDLLFFKATEERVRFDLDKITGTVPIVKAYAGMGDSGII
                     SFLNSQNISGLVIEALGAGNMPPKAAQEIEELIEQGVPVVLVSRCFNGIAEPVYGYEG
                     GGAKLQESGVMFVKELNAPKARLKLLIALNAGLTGQNLKDYIEG"
     gene            complement(26995..27873)
                     /gene="aroE"
                     /locus_tag="VBC74_000358"
     CDS             complement(26995..27873)
                     /gene="aroE"
                     /locus_tag="VBC74_000358"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEO6941336.1"
                     /translation="MNSLNGETLLTGLIANPARHSLSPLMWNTSFQEKNMNYAYLTFE
                     VEEGKLTEAVRGVRALSIRGVNVSMPFKQSVIPLLDDLSPQAKLVGAVNTIVNQGGTG
                     RLVGHMTDGIGCFKALAAQGFSAKNKIITIAGIGGSGKAVAVQAAMEGVAEIRLFNRN
                     SSNYDKVIDLSDKIKKQFQIKVVVDYLENKTAFKDAIRTSHFYIDATSLGMRPLDNYS
                     LINDPEILTPNLVVVDLVYKPKETALLRFVRQNGVKHAYNGLGMLIYQGAEAFQLITN
                     QEMPISSVERVLQTEN"
     gene            complement(28010..28885)
                     /locus_tag="VBC74_000359"
     CDS             complement(28010..28885)
                     /locus_tag="VBC74_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6941337.1"
                     /translation="MLQNIGQTGIQATRIALGCMRMSDLKGKQAEEVVGTALDLGINF
                     FDHADIYGGGLSELRFRDAIKHLNVNRDKMIIQSKCGIREGYFDFSKEYILSSVDGIL
                     ERLGTEYLDFLILHRPDVLVEPEEVAEAFTKLRAEGKVKHFGVSNQNRFQMELLQSYL
                     DEPLAVNQLQLSPAHTPMFDAGLNVNMLNKASIEHDDGIVDYCRLKRVTIQAWSPFQI
                     DLSRGLFVNHPDYKELNETIAKLAKNYNVSSEAIVIAWILRHPAKMQAIVGSMNPSRL
                     KAIDKANDIALTRKE"
     gene            complement(29176..31191)
                     /gene="recG"
                     /locus_tag="VBC74_000360"
     CDS             complement(29176..31191)
                     /gene="recG"
                     /locus_tag="VBC74_000360"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEO6941338.1"
                     /translation="MLLQSPISNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVSQGISQSTLIKAIKSAFE
                     INAHLELKENLPATLLGKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKAENKSETNGLSILYSKHAMETKISSLPFILTNAQKRSLDEILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSIIDELPAGRKPIITRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     YKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARRVASDIVKDNNWKENTEWALILDNLRQHSDFD"
     gene            complement(31271..32809)
                     /locus_tag="VBC74_000361"
     CDS             complement(31271..32809)
                     /locus_tag="VBC74_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6941339.1"
                     /translation="MSKQKVTATLLLSTLVLSLSSPLVTLAETINPETSLTMATASTE
                     SSSEAEKQEKTQPTDSETASPSAEGSISTEKTEIGTTETSSSNESSSSSSHQSSSNED
                     AKTSDSASTASTPSTNTTNSSQADSKPGQSTKTELKPEPTLPLVEPKITPAPSQIESV
                     QTNQNASVPALSFDDNLLSTPISPVTATPFYVEHWSGQDAYSHYLLSHRYGIKAEQLD
                     GYLKSLGIQYDSNRINGAKLLQWEKDSGLDVRAIVAIAVLESSLGTQGVAKMPGANMF
                     GYGAFDHDSSHASAYNDEEAIMLLTKNTIIKNNNSSFEIQDLKAQKLSSGQLNTVTEG
                     GVYYTDNSGTGKRRAQIMEDLDRWIDQHGGTPEIPAALKALSTASLADLPSGFSLSTA
                     VNTASYIASTYPWGECTWYVFNRAKELGYTFDPFMGNGGDWQHKAGFETTHSPKVGYA
                     VSFSPGQAGADGTYGHVAIVEEVKKDGSVLISESNAMGRGIVSYRTFSSAQAAQLTYV
                     IGHK"
     gene            complement(32902..34002)
                     /locus_tag="VBC74_000362"
     CDS             complement(32902..34002)
                     /locus_tag="VBC74_000362"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO6941340.1"
                     /translation="MISSYHRPTRALIDLEAIANNVKSVQEHIPSDKKTFAVVKANAY
                     GHGAVEVSKYIESIVDGFCVSNLDEAIELRQAGIVKMILVLGVVMPEQVILAKNENIT
                     LTVASLEWLRLCQTSAVDLSGLEVHIKVDSGMGRIGVRQLDEGNKLISELGESGASVK
                     GIFTHFATADEADNCKFNQQLTFFKDFISGLDNCPDLVHASNSATSLWHSETIFNAVR
                     LGVVMYGLNPSGTDLDLPYPINPALSLESELVHVKQLHDGSQVGYGATYQVTGDEFVG
                     TVPIGYADGWTRDMQGFSVIVNGELCEIIGRVSMDQMTIRLPQKYTIGTKVTLIGQQG
                     SCNITTTDVAQKRQTINYEVLCLLSDRIPRYY"
     gene            complement(33999..34358)
                     /locus_tag="VBC74_000363"
     CDS             complement(33999..34358)
                     /locus_tag="VBC74_000363"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000635015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEO6941341.1"
                     /translation="MIVGHGIDLQEIEAITKAYERNQRFAERVLTEQELLLFKGISNP
                     KRQMSFLTGRWAAKEAYSKALGTGIGKVNFHDIEILSDDKGAPLITKEPFNGKSFVSI
                     SHSGNYAQASVILEEEK"
     gene            complement(34383..35390)
                     /locus_tag="VBC74_000364"
     CDS             complement(34383..35390)
                     /locus_tag="VBC74_000364"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEO6941342.1"
                     /translation="MGFHQLSDKINIEILKQKTSLDLEVSQKKLAKEEELKNIIKGED
                     QRFLVIVGPCSADNPKAVLTYAKRLAKLEAAFKDKMFLVMRVYTAKPRTNGDGYKGLV
                     HHSDKLGVFFQARKMHYDIIRETGLLTADELLYPEMLSVMDDLVSYYAIGARSVEDQG
                     HRFISSGIDAPVGMKNPTSGNLRVMFNAVYAAQNQQELFYQNKQVRTDGNLLSHVILR
                     GYHNADYRSIPNYHYENLLETITHYEETDLQNPFIVVDTNHDNSGKQFLEQIRIVKSV
                     LADRQWHTKIRNYVRGFLIESYLEDGRQDKPDVFGKSITDPCLGWDKTEMLIRDIYNS
                     L"
     gene            complement(35516..38044)
                     /gene="secA"
                     /locus_tag="VBC74_000365"
     CDS             complement(35516..38044)
                     /gene="secA"
                     /locus_tag="VBC74_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEO6941343.1"
                     /translation="MANILRTVIENDKGELKKLDKIAKKVDSYADHMATLSDEALQAK
                     TPEFKERYQNGETLDQLLPEAFAVVREASKRVLGLYPYHVQIMGGIVLHHGDIPEMRT
                     GEGKTLTATMPVYLNAISGLGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PFEKREAYNCDITYSTNAEVGFDYLRDNMVVRQEDMVQRPLNYALVDEVDSVLIDEAR
                     TPLIVSGPVSSEMNQLYTRADMFVKTLNSDDYIIDVPTKTIGLSDTGIDKAENYFHLN
                     NLYDLENVALTHYIDNALRANYIMLLNIDYVVSEEQEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKESVPIQEESKTSASITYQNMFRMYHKLAGMTGTGKTEEEEFREIYNMRVIP
                     IPTNRPVQRIDHSDLLYPTLDSKFRAVVADVKERYEQGQPVLVGTVAVETSDLISRKL
                     VAAGVPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGTDRIKVVLERMNLAE
                     DDTVIKSKMLTRQVESAQRRVEGNNYDTRKQVLQYDDVMREQREIIYANRREVITAER
                     DLGSELKGMIKRTIKRAVDAHSRSDKNTAAEAIVNFARSALLDEEAITVSELRGLKEA
                     EIKELLYERALAVYEQQIAKLKDPEAIIEFQKVLILMVVDNQWTEHIDALDQLRNSVG
                     LRGYAQNNPIVEYQSEGFRMFQDMIGSIEFDVTRTLMKAQIHEQERERASQHATTTAE
                     QNISAQHVPMNNESPEYQGIKRNDKCPCGSGMKFKNCHGLRCLQ"
     gene            complement(38153..39100)
                     /gene="manA"
                     /locus_tag="VBC74_000366"
     CDS             complement(38153..39100)
                     /gene="manA"
                     /locus_tag="VBC74_000366"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEO6941344.1"
                     /translation="MSEPLFLEASMHDKIWGGTKLRDEFGYDIPSETTGEYWAISAHP
                     NGVSRVKNGRFKGCFLDKLYQGEKSLFGNPDDTVFPLLTKILDANDWLSVQVHPDDAY
                     ALKHEGELGKTECWYIISADEGSEIIYGHNAKTKEELRQMIESGDWEHLLTRIPVKSG
                     DFYYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRPDASGKLRDLRIEQSIDVLTI
                     GKPANTVPANMKLKHLSSTLLVSNDFFTVYKWEISGVTNFKQFAPYLLVSVLDGAGHI
                     TVDNKVYTLKKGDHFILPNDVVKWDIDGQLEIIASHVKK"
     gene            complement(39218..40099)
                     /locus_tag="VBC74_000367"
     CDS             complement(39218..40099)
                     /locus_tag="VBC74_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO6941345.1"
                     /translation="MTKLYGSIEAGGTKFVCAVGDEELKVVEKMQFPTTTPQETIKKT
                     VDFFKRFEKKLEAVAIGSFGPIDIDKKSKTYGYITTTPKLHWANVDLLGLISKDFNVP
                     FYFTTDVNSSAYGEVIARNNIDSLVYYTIGTGIGAGAIQKGEFIGGTGHTEAGHTYMA
                     MHPQDQANDFKGICPFHNSCLEGLASGPTLEARTGIRGELIEENSMVWDVQAYYIAQA
                     AIQATVLYRPQVIVFGGGVMAQEHMLRRVRQTFATLLNGYLPVPDLSDYIVTPAIEEN
                     GSATLGNFALAKKISKG"
     gene            complement(40167..42086)
                     /locus_tag="VBC74_000368"
     CDS             complement(40167..42086)
                     /locus_tag="VBC74_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEO6941346.1"
                     /translation="MASNTEIAKQVINAIGGASNVRSVAHCATRLRIMVKDETVIDKN
                     TVENIEKVQGAFFNSGQYQIIFGTGTVNKIYDEVVAQGLPTLSTSDQKAEAAKQGNAF
                     QRAIRTFGDVFVPLLPAIVATGLFMGIRGAINNDTVLALFGTTSKAFSSSNFYTYTVV
                     LTDTAFAFFPALISWSAFRVFGGNPVIGLVLGLMMVNSALPNAWAVASGDAHPIKFFG
                     FIPVVGYQNSVLPAFFVGLLGAKLEKWLHKKIPDVLDLLLVPFLTFTVMSILALFVIG
                     PIFHSVENYVLAGTKFVLNLPLGLSGLILGGVHQIIVVTGVHHIFNLLEAQLIAADGK
                     DPFNAIITAAMTAQAGATLAVGVKTKNKKLKALAFPAALSAGLGITEPAIFGVNLRFG
                     KPFIMGLIAGAAGGWLASILKLAGTGFGITIIPGTLLYLNGQIVKYLIMVIGTTSLAF
                     VLTYMFGYEDKDEKAVAEVSPLVEETDDDPTITQTSQLRAETIVSPLDGQVIALDTVS
                     DPVFSSGIMGDGLAIKPRGNTIYSPVDGFVQIAFETGHAYGIKSDKGAEILIHIGIDT
                     VTMNGTGFTSKVKADQKVKKGDILGTFDSAKIAEAGLDNTAMIIVTNTADFADVIPLV
                     KEGVVTTGDMIIDVK"
     gene            42271..43710
                     /locus_tag="VBC74_000369"
     CDS             42271..43710
                     /locus_tag="VBC74_000369"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEO6941347.1"
                     /translation="MNLPTEIRYRPYDEWTEEDKENILKNVSKSPWRATYHLEAKTGL
                     LNDPNGFSYFNGKFHLFYQNWPFGAAHGLKQWVHTESDDLVHFKETGIKLKPDHVNDS
                     HGAYSGSALAIDDKLFLFYTGNVRDMKWNRDPRQIGAWMTSDGKITKFDKVLISQPND
                     VTEHFRDPQIFNYDNQFYAVIGAQNSKKCGFIKLYKALNNDIHHWEFVGDLDFGGTGS
                     EYMIECPNIIFVKGKPVLLYSPQGLDKNELDYQNIYPNTYKIGQYFDANSSKIVEPSP
                     IYNLDYGFEAYATQGFNTSDGRAFIVSWIGLPDIDYPSDQFDYQGAMSLVKELSIKNG
                     NLYQYPVPAMKNLRQHQAEFKTQLQTNNTYELELLVPRNDLSSFVLFANPKGQGLSIT
                     IDTAKGKVIVDRSQAGQQYATEFGTSRQCDIPKDATSINIFIDKSIFEIFINKGEKVF
                     TGRVFPDAEQSGIQLKEGHVHGKYFELKY"
     gene            43712..>44661
                     /locus_tag="VBC74_000370"
     CDS             43712..>44661
                     /locus_tag="VBC74_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6941348.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINKKGYLSQKTVTKVNEAMRTL
                     GYKPNNLARSLQGKSAKLIGLIFPNIRNIFYAELIEHLEIELFKHGYKTILCNSEKDP
                     IKEKEYLEMLGANQVDGIISSSHNLGIDDYEKVEAPIVAFDRNLAPHIPIISSDNFFG
                     GKMAAQTLKKHGCQKMIMITGNDNSDSPTGLRRLGFSYESKESKVITVTNGLSNMRRE
                     MELKSIISTHKPDGIFTSDDLTALLVIKLISQLGLSIPEDIKVIGYDGTSFIQDYVPH
                     LTTIKQPIREIAQLMVEILLAKIEGQKTNKDYILPVSLIPG"
BASE COUNT        14094 a         8942 c         7154 g        14471 t
ORIGIN      
        1 attctgtttg gaaaatccat tctaataaag catatttatc aacaaagtga ttataaaatg
       61 tttgacgtct aatacctgca tcttccataa tatcactcac cgatattttg tcaaaagctt
      121 gcgaaataaa taaacgttta aatgacttgg ctattttttt cttagtaata agcgaagaag
      181 tcataatttc tcctttattt tatattgaat tgctgatgat ttattatggt ttagtgtaac
      241 atgatttatg attattgaaa accctaacaa agcggacaaa caagaaaatc tgtccattcc
      301 agaaaaggct tacaaatttt ataataaaag agaattaaac ataaaaggag tttccaaatg
      361 aaaaaaattc tcaatcaacc aactgatgtt gttactgaaa tgctagatgg attagcatat
      421 gttcataatg atttagtaca tcgcatagaa ggttttgata tcattgctcg caatgaagag
      481 aagtcaggaa aagtggctct catttctggc ggaggaagtg gacatgagcc ttcacatgct
      541 ggtttcgtag gcgaaggaat gttatctgca gctgtgtgtg gtgcagtatt tacttctcca
      601 actcctgacc aagttcttga agcaattaaa gaagctgatg agggggctgg agttttcatg
      661 gttatcaaaa attattcagg ggatatcatg aactttgaaa tggcacaaga tatggctgaa
      721 atggaaggca tagaagttgc aagtgtggtc gttgatgatg atattgcagt cgaagacagt
      781 ctttacacac aaggaaaacg tggtgtagct ggtacgatac tcgtccataa aattctagga
      841 catgctgctc gtcatggcaa atcacttcag gaaatcaaag ctattgctga tgaattagta
      901 ccaaacattc acactgttgg acttgcacta agcggggcta ctgttcctga agtaggcaag
      961 ccaggatttg ttcttgcaga agatgaaatt gaatttggta tcggtattca tggtgaacca
     1021 ggttatcgta aagaaaaaat gcaaccttca aaggctttag cgacagaatt agttgataag
     1081 ttaattgaat cgttcgatgc taaatctggc gaaaaatatg gtgtccttat taacggtatg
     1141 ggtgcaaccc cacttatgga acaatatgtc tttgctaacg atgttgccaa actccttgag
     1201 gataaaggca ttgaagtgaa ttacaaaaaa cttggtaact atatgacttc aattgatatg
     1261 gctggtttat cactaacctt gattaaactg gaaaatcaag agtggttaga agcacttaat
     1321 agtgacgtta caaccattgc ttggtaatca ttcctgttaa ttagaacaaa aggaaggaac
     1381 tcttatggaa gtaaaaactg caatagaatg gatgcacact tttaaccaaa aaattcaatc
     1441 caacaaggac tatttatccg aactagatac tccaataggt gatggcgacc atggtggcaa
     1501 tatggcgcgt ggtatgactg ctgttattga aaaccttgat aacaatgaat ttagtagtgc
     1561 tgccgatgtt ttcaaaaccg tgtccatgca acttttaagc aaagtaggcg gagcatctgg
     1621 accactctat ggttctgcat ttatgggaat tacaaaagct gaacagagtg aaagtaccat
     1681 ttcagaggca cttggcgcag gacttgaaat gattcaaaaa cgtggtaaag ctgaacttaa
     1741 tgaaaaaaca atggttgatg tttggcacgg tgttattgaa gcgattgaaa aaaatgaatt
     1801 gactgaagac cgtatcgatt ccttagtaga tgctacaaaa gggatgaaag caacaaaagg
     1861 acgcgcttct tatgttggag aacgctcagt aggacacatt gatccaggat cattttcttc
     1921 aggattatta tttaaagcat tactagaagt tggaggtgtc taatgtctga tattggtatt
     1981 attgttgttt ctcactctaa aaatattgct caaggagttg ttgatcttat ttcagaggta
     2041 gcaaaggatg tctcaataac ttacgtgggt ggaacagaag atggtgagat tggtacttct
     2101 tttgatcaag ttcaacagat tgtagagcaa aatgataaaa agacgttatt agctttcttt
     2161 gatttaggtt cagcaaaaat gaatttagag ttagtagctg atttttcaga aaaaaatatt
     2221 atcattaact ctgtcccagt agtggaggga gcttacaccg ctgctgctct tctacaggca
     2281 ggtgctgacc tggatagtat tcaatcacaa ttagctgaat taactatcaa taaataagga
     2341 gatattatga ctcaatttct tggggaattt ttaggaacat ttattttggt tcttcttggg
     2401 gatggcgtag ttgcaggaaa tgtgctcagt aaaaccaaag aagaaggaac tggatggact
     2461 gctattgttt ttggctgggg aattgcttgt accgttgctg tttatgtttc tggattattc
     2521 agtcccgctc atctaaatcc agcagtcaca ttagcaatgg cttctattgg agtaatttct
     2581 tggggacaag ttatcccctt tatcattgca cagatgttag gagcaatggt tgcggcaact
     2641 attttatggt tacactatta tcctcattgg aaagaaacaa aagatagtgg acttatcctt
     2701 gccagcttct caaccggacc tgctatccgc catacaccat cgaatctcct tggtgaaatt
     2761 atcggtactg ctattcttgt gattacaatt atggcaattg gtcctagtaa agttgcagca
     2821 ggtcttgatc caattatcgt tggtatagtt attttcgcag taggattctc tcttggtcca
     2881 acaactggtt atgctattaa ccctgcacgt gacctagggc ctcgtcttat gcatgctatc
     2941 ttacctattg aaaataaggg taactcagat tggtcatacg cttggattcc agttgtaggt
     3001 ccaattatcg gcggtgtcct tggggctatc ttatacaata ttgctttgac tttagtttaa
     3061 ctaaaaaacc agaaagttaa tactttctgg tttttcatag ttgcagtatg ctttcaaaat
     3121 agaaaaaaca ccaaaatact tagtgttctt tctggagatt ttatgaaaaa agtttaggat
     3181 gtttatagta taaccgccct tacttaacgt ttccttaaaa gctcggttat taacattact
     3241 tcctaaatta ctatttatat tctccaagaa atgactacta ttaagctaaa aagcgctatt
     3301 gacatagcgc tttttagtct agtattattt agtttcagta gtaactccat caaaatcttc
     3361 ttcattttct tcagcttctt ctcttgtggc acggataata ccatctttgt gatgagctgg
     3421 actgtaaatt gtatagagtt ttaacatctc agtttcagaa gtgttaatta cgttatgttc
     3481 agcacctgcc ggaactatta tagcgtctcc atctgcaact tcatattcat taccatcaat
     3541 gacaacctta ccatgaccag cttcaaaacg gaaaaactga tcattttctg catgaatctc
     3601 gctaccaatt tctccaccaa caggcagtgt cattaaaacg agttgacaat gttctgcggt
     3661 atagaggact tggcggaaat ttgtatttcc tagtgtcaat ttttcgatat tcccatgaaa
     3721 tcctttcata acgataccta cctttctatg cctacagtct actctttttg ctccttactg
     3781 tcaaaaattt agataagagt ctgacatttt taataatatc tactgttcct tgattcctat
     3841 catttcattg caaacctaaa atatagccta atgacaatag ctatctatag ctttagaaac
     3901 aaataatgct gttccctggc ctccccaatt atcaatagac ttttggaaac gctcatctta
     3961 gatgtacatt atttcaagac ttgctaataa atcgttagtg cagttgtaaa actgagctgt
     4021 gatataatct tgtaaaatct gaacttgttt ttgaactttc tcatctgtcg gagaaagttc
     4081 tttcaactgt ccaaagtctt taaaaatctg actcatagct tgtgaaatca ttgaaaaatc
     4141 attcttacta tggctatttt caaattcttt gtaactatct gtatcccccc cagcgtgttc
     4201 tagcttcctc ttgaaatact tctaattctt cttgattata agctgtaaaa tctgacatat
     4261 ttttacccct ctggatagac tttgcatgtt caattacttc ttctagtcgc tgtttcttta
     4321 gttccagcca cctaatttga tcaagaagtg cttgattacg atcataattt ggactttcca
     4381 ttatctcttt tattttttta agaggaaatt ccagttcctt aaataacaaa atttcttgta
     4441 acttaatcag tgattcatca tcatagtaac gataaccatt ctctcctaca aatgaaggag
     4501 ataacaaatc tatttcatca taataatgaa gtgtgcgcac actaatacca gaaatgtgac
     4561 ttatttcttt aactgttttc ataataacct ctctgtaaag agtataatgt ataacgttaa
     4621 gtaagggtca agggaaaaat attattttgt gatgattaat ttataaagct taaatcaaag
     4681 gtcactttga ctaacaggtt gataaaaagg gatacgtccc catgtttgct ctgctatatt
     4741 agcatttccc atctgataaa cagaatctgc tcgttgcgtc aaactatccc acgtttgtga
     4801 accaatcctc gtcacgatat ccacagactt cttcacagac tttaaatgct gttgaccttt
     4861 ttgtgtaaaa cctaagacat gaatagcatt tggtatagga ctttccacag catttatcaa
     4921 gatataggtt aataaacgtc gaattcttgc tttggtatac cgtttagttg cgactttatc
     4981 aactaactca tccacagtct ccacataacg aatttgactt tttatacgat tggctatctc
     5041 ttcattcact tgataaattt gtgtcaaatc agaatgcgtc attatctgat atttgagtaa
     5101 actaaaataa tcttgccaaa ctacttgtgg actattcaaa aacaagtcac tgttaggcat
     5161 acctttttct acaaagaaac gatcagattg atgtttacga agacttgttg cagaagcaaa
     5221 tatctctgtt ttttctgatg aatgataacc agctccttga cgttgtatcg gatttaatcg
     5281 aataccattt tggctagctg ctttggtgta ggctaagcct aaaatgtgat ttggggtttg
     5341 tcccgaaaat gtaatccctg caaattcttg ccacatagct tgcatttttt gtggatagga
     5401 aagagaatcc gattgttttt tcaaaaaagc ttccatctcc tcttttttat tcacataaat
     5461 atccccaata cgggcataat caagcatttc ttcggtaccg aagcataagt tatccacacc
     5521 taatcgtgcc aggatagaca cagctccact agcgaaataa tccgctgact gtaccgaaac
     5581 cagaaaaggg agttcaatca ctaaatcagc accattttct agtgccatct gggcacgggt
     5641 ccatttatct acaatcgccg gctcaccacg ttgcataaaa ttacccgaca tggctattac
     5701 ttttatgcct tgagcttgtt ctaaaaggta cttatgccca ttatgaaagg gattaaattc
     5761 ggcaacaatt cctgtaactg tcatacttct ttctctttca attgattatt taaagtaaca
     5821 gcatcaataa tatctaggtg ataagtttta cgaaggtata tgagacgatc tgtttctttt
     5881 tcaaatggtt ttaatgccga taataaatcc aaatgaagtt tagggttcat caataattcc
     5941 aaaataactt tagacttttt ctgacttacc cgacgttcaa taatataaag tatcccaaat
     6001 atcatcagac aagcgtataa gaatattatc atctttttac ctccaaatta ttacttattt
     6061 atgagctaca aaaaaccagc gagcagaagt tgcagttggt tttttatctt caaaatctgc
     6121 atacacctta acatctttaa aacctgcctg ttcaagtaaa atatcataag tcaaaatatc
     6181 ataagtacgc tcttcatgaa cttcatcatg acgtgtaaat cgaccatctt cttcttgaac
     6241 aaaaaatgtt aattcatgga caatagaatg aggtgcatca tcttcataag tatcccacac
     6301 catagcaaag tcatctgcat tttcatgata agaataacca ggaaatactt tatctgtttg
     6361 ataagtcgaa tgaacatcaa aaataaagac gccattttct tctaaggcct tatagacttc
     6421 gataaaaaca tcaccaacct cgacttcatc ttgcatataa caaatagagt ctgaatagca
     6481 cgtgatgaga tcatatttac caacattaga taaatctaac atatttcctt caataaactg
     6541 gatactttgg tgagcagaag ttgcacgttt tttggcaagt ttcagcatat cgccactaag
     6601 atcaagtcct gtaacagcat aaccagcttg tgcaaatcga acagactgaa ttcccgtacc
     6661 acatgctaat tccaaaagtt ttttcttacc ttttggaaaa tgacgaagtg aaaaatcagt
     6721 ccatttatca tataaagtat catccatgac agcatcataa acagctgcaa aagtttcata
     6781 cgttgccata taatagttct caaatctttc ttgatattac atctctcaaa aagcagtccc
     6841 aagactgctt agctttattc tgtcataaag acttcagcat caagcaatgg tgcttcatgc
     6901 caaagttttt caaggttata gtgctgtctc tcatcttcag agaagatatg aaccacgacg
     6961 ctattgaggt caagaagtac ccaaccagct ttactatcgc cttcaaggtg gctagcatcc
     7021 ccaccatttc cttttacttg ttcacggata ttgtctgcga ttgcttctaa ctgcctacta
     7081 ttacttgcac tcataatcac aaagtaatct gcaacactcg ttacaggctg taaatctaaa
     7141 attacaatat cttctgctcg tttttcatcc gcagccttaa cgactagctg tagtaagtct
     7201 ttttctgtca ttttattcct cgtttattaa tttcaataag ctatcttttg ctaccattgt
     7261 cgcaaaacga tgatgccaaa tattctcgta atgtttttta atagaatccc cacttaaagt
     7321 tggtgtatca aatgttaggt gtccaccttg tggaataaag atatcataac catattcaaa
     7381 agcaactttt actgatgtat caaaacagta ttctgtttga agtcccatca tgattaattg
     7441 actaatatgc ttactttgca agtaatcatg taaatgagta tctttaaaac aactattgta
     7501 agttttatca attattttgt gatgcgtcac aagatgtcgc tgttcccaaa tttcccactg
     7561 tggagaacct tgaggcaatt ccttatcatg atgccttatc catatcactt cacaattaga
     7621 acctttggca cttaaaagta aatcatctaa aagctctgca aaatttgtta aatgctttgg
     7681 tttcttatcc ataatacctt gttggatatc aattagtaaa agtgcacaag tcatactttc
     7741 ctccaaaatg accactgcat cgccaaacct atcatgacca ttattcaata aaagaattag
     7801 tcgagataag gtatataagc attataggtt tcaatcgttt taggatagat aggctgagcc
     7861 ttacttgcta aaaacgcaac tgttctagca gtctcataag ctactgcttt attaaggtca
     7921 actttcgcta actcacgagc ttcttcaact cctggaaaat ctcgattgtg ttcaatgtaa
     7981 tctgctacat aaacgatttt atccaatgtg gacatttggg ctgaaccaac agtatgtttt
     8041 gcaatagcag ctaaaatatc ttgatctttg atagctagat cctcttgtat tttataaatg
     8101 ccaacaagac catgccaaat attattaccc cattttttta ggtcgggatc tagttggtat
     8161 ttatcaatta atctaagaaa ttcatcatca gatagttcct tagcataatc atgtaataaa
     8221 gcagctagac ccgctttttc cttgtcataa ccataccgtt ctgctaactc tattgcagca
     8281 cgctctactc ctaacacatg attaaaacgt ttgtcggaca tcatatgacg cactttactc
     8341 aaaagttcag tccgatctaa acctgtgtaa tctttgtagg tcatttgtaa agaccttctt
     8401 tcctgatata atctaatacc tctctaggca gtaaatagtt gggctgacga ttacttttaa
     8461 taaattgacg aatcatactt gatgagatat ccatcaatgg cagatccacc caaataacgg
     8521 gatatgatgt accagcttta tattttggac gttgaacacc aacaaactgc accattttaa
     8581 caagttcatc aatacgatgc cacttaggta gatattccac catatctgcc ccaataatga
     8641 aataataatc aacatctgga tttttttcaa ttagaagctt catcgtatcg tatgtatagc
     8701 tgatacattt acgttcaatt tcaattggtt caatagataa accatcaatg ccttctattg
     8761 ctaattcaag catcttgaga cgatgctgtt catcaattgt ctctttttta tcaatatgtg
     8821 gtggctgaaa ttcaggcatc aacagaactt ggtctaaaca caattgctga cgtacttgat
     8881 ctgcaacaac aaggtgggcg ttatgaacag ggttaaaatt tcctcccata attccaattt
     8941 gttttcggtt agtatctcgc tttttctctt ctaattcaac ttttgtaaat ggtgttagca
     9001 attcgagtgc cataattcct ccatgaacaa gtagtatctc taaaaaaaca aacactgatt
     9061 atcatgatca gctatcatac ggaagtgtaa cttaggtaaa tcgtttttta gttctatact
     9121 ctatttctta atctattgta gtttagtttg attttactgc ttttacttta actgaaattt
     9181 tacgattttc tttgcgcgct gactctttat aaagaatcaa gatacgtcct attttaagga
     9241 cagtatcgca gccaatttca tcttccagaa cttcagcgac atcgtggata tcttcatcgg
     9301 tattttgtaa aagggttact ttgattagct cacgtgcatc gagtgcatta cgtacgcttg
     9361 ttttgatttg atcatttaaa ccatttttcc caatttgaat aatgggtttc atagaatgtg
     9421 cttctgattt taagaaagca cgttgtttag atgttaacat attagtttcc tttttatcta
     9481 ttataggatt atgactgtat catcagctaa attagatgaa aaaccgtcat atgttgctgg
     9541 ttaatttagt tattaaatta aagctttgcg taaaacaact gccacgcctt ctggagccca
     9601 agcagctact actactggat tttctgcttc tgatttcaca cgtatccaac caagacccga
     9661 ataaacaata tccattttat ctttgattgt aaattcatga cgaactaatt taggaaaatc
     9721 tgaaacttcc ttacctgttg gtggtgtcaa taattttcca acatgtttag tgtaaaattc
     9781 atctgctcca actagtttag tgcggtgtaa attcaggtta ttatcaaaat atgccgtaaa
     9841 accttgtttt tgaccagaaa taaaatcaaa acgtgctagg ccagccaaga ataatgtttg
     9901 ttctgaatta agctgataag ttttgggttt tatttctttt ttaggactaa catatttaag
     9961 attttttgct gtcaaataat gagccatttg atgacgatga ataatacccg gggtatcaaa
    10021 aatatagcta ccatcatcaa gaggtatttc tattttatct aacgttgtac ctgggaaacg
    10081 tgaggtcgta ataacatcac gactaccagt aatttcacga ataatagcgt taataagagt
    10141 tgactttcca acattagtaa ccccaacaac ataaacatct cgaccatgac gatacttttc
    10201 aatcgtatca atcaaatcct taatagcatg atgattttgg gcacttgtca agatgacatc
    10261 aacaggacga agcccctctt catgagctct ctcagttaac cattgtgtta cttttccatc
    10321 tttaaccgat ttgggaagaa tatctttttt atttcctact aaaagaacat cgtttcctgc
    10381 aacgaaacga ctaagaccag gaataattga accattgaaa tcaaaaatat caatgacatt
    10441 caccacaagg gcatcactat ctccaacctc atgtagtaat ttcaaaaatt catcatcagt
    10501 gatatgaacg tcagtaatct cattatagtg gcgcaatctg aaacaacgct gacagtataa
    10561 ttctcctgtc tctaaacctt tttcaagtgc tgcacgaggt gtatagccag cagcatcttt
    10621 attttcagtt tgaatcctgg ctccgcagcc aatacaaaat aactcttcca ttaaattcct
    10681 ttttgatata cgattttacc gtaattctct tctatttttt tccaaacgcg acgttcacgt
    10741 aagcgattga acttggtatt ccaagcatca gatttaacaa ttggttttac caatacggac
    10801 tttattcctg cgcgataact agcacgaata tctgtcatca attgatctcc aaccataatc
    10861 acctcgtcac gatcaaagcc ataacgctca attgccatgt ttataccacg agtaaatggt
    10921 ttcatcgcac gacttacaaa atccacacca aaacgtgata ctgcacgttc tacgcgagca
    10981 tgattgttat tagacactac tacaactgaa atatccgctg ttgtcatctc atctaaccat
    11041 gcacgcacct cagcagtacc atctggatta ttccaagcaa tgagtgtatt atctaaatca
    11101 actaaaaccg cacgaatgcc atgacgtaat aaatcttctg ctcttaaatc ataaactgcc
    11161 tcaaccacga aggtcggcct ataatcatct atactcaatt tcatctccaa aaaatatatt
    11221 ttactattca ttctaccata attcttaaga gatagcgagt aaagaaaaaa ggtctttaaa
    11281 gacctttaaa ttagggtttc aaccccaaaa gatactatct tactcgtttc attttcttca
    11341 atgcaattaa atctttgtaa gagattagag ttacagcaag aaaaataaac agcatcccaa
    11401 gtaaatctat tggataaaaa atttctccta aaacaagtac tgtcaaaaat accgaagaaa
    11461 ctggctctac cgatgctaat aagctaccct tgacagcacc tacaatacta acccctttaa
    11521 ggaaaaaagt gtaagcaaaa atagtcccaa taccaataat gccaatataa gcaagtatac
    11581 tcatcacatt aatttgtaag gggtattgcc acgcttttgt caccaaacta aataaaatcc
    11641 cacctataaa cattcctgaa ccaataacaa tcgtacttcc ccattcatga ataagtttca
    11701 caggtaataa aatataaata gaataagtaa tggcggaacc aagcccccac attaatcctt
    11761 tcggtgtgat agctaaactt cctaacttcc catgcgttgc aatcatatag gttcctaaaa
    11821 ttgccatagc aattgctatt atttcaaaag atgttggtct ttgtctgttt aggatacaaa
    11881 caatactcaa aataagtatt ggtgccatat attgcaaaac cgttgcagtt ccagcatttg
    11941 ttaaatgaat cgctctgaga aaagcatatt gatttaaaac taaaccaaat atagaaaaaa
    12001 gtaaaacctg ctttaaaaat tttggctgtt tccacgctgc tactaaatgc tccttttgtt
    12061 ttgatctagc taaagacaag agaaagatac ctgttatcaa caatcgtaaa gaagtcaaaa
    12121 ggttaacgtg tactccgtgc gacatcaagt attgaccact aattccagaa atgccccatg
    12181 ctagtccggc agctaaggtc atcattgttc ctttttcttt tttagtcatt tttcccctca
    12241 tttttatttc ttaaacactg tagttaaagt catctgataa atatcttagc ttcttaatta
    12301 tttatcccta ggtgttcgtg ctaaattagc gaataaaaac cagccaaaag gctgatttct
    12361 attcttcttt taattttgct aattcttgcg ctaacagctt cagagcaact tgtggattag
    12421 cttgtccttt tgttgctttc attagataac ctgtgaaggc cttatcagca ttgcgttttc
    12481 ctgacttaaa gtcaatcaca gcagcttcat tatctgcaaa gacttgatga ataattggga
    12541 ttaatacttc aggatctgaa atttgaacta aaccagcctt tttaacgaat tcttcagctg
    12601 aaccaccatt tttagctaaa tgcacaaaaa cttttttagc aattttggat gaaattgtac
    12661 cgtcagcaat caagccaatc atctcaacca aattctcagg agtcagacca atttcttcga
    12721 ttgattttga ttcactgtta agaaattgcg ccacttctcc ttgtaaccaa ttggaaactt
    12781 gttttgcatc acccccaata gcaacagctt tttcaaagaa atcagaagtt gcttttgtag
    12841 ctgttaattg gctagcatca taagatgata gaccaaaaga cgatacatat ttcgcacgac
    12901 gttcctgtgg aaattctggt aattctaaac gaacttgatc aatccattca tccgagatat
    12961 caaatagtgg tagatctggt tcagggaaat agcgataatc tgatgagcct tccttgacac
    13021 gcatcaaaat agtctcacct gttgtttcat caaaacgacg agtctcttgc tgaatttgac
    13081 caccagatcg taaaacttgt gcttgacgtt tttcttcatg tatcaaacct ttacgtacat
    13141 tattgaatga gttaaggttc ttaagttctg ctttagtacc aaactcttct tgaccataag
    13201 gacgaagtga aatattggca tctacacgca tagaaccttc ttccatttta acgtcagaaa
    13261 ttccagtata ttggataatc tctttgagag ctgtcaaata agcataagct tcctcaggag
    13321 aacgcatatc agcttctgat acaatttcaa tcagtggaac accttgtcta ttaaggtcaa
    13381 cataggaata accatctgtc ccatgtgtat tttttcctgc atcttcttcg aggtgggcac
    13441 gctcaatacg aatttttttt cgtgtaccat cttctaattc gatttcaatc caaccattgt
    13501 aaccaatagg ctcatcaaac tgtgaaattt gataagcttt aggattatct ggatagaaat
    13561 aatttttacg atcaaaatgc atgttttgat ggatatccat attcaaggca agtgccgcct
    13621 tgattcctgc atcaataact cctttattca tgactggaag gacacctggg aaagaccagt
    13681 caattacatt tgtattagca ttttgttcct gaccaaaatg agcggaagaa ggtgagaaaa
    13741 ttttagagtt cgtatttaat tcaacatgga cttcaagtcc aatgactgtt tcaaaattca
    13801 ttagttctca cctccaaaaa ttttaggttg ttgtttatga taatctgtcg ttgcttcgaa
    13861 ggcagcagca acctgatata ttgtttcttc actgaatttc ggaccaatca actgcatacc
    13921 aaccggtaga ccttgatcaa aaccagcagg gattgaaata cctggtaaac cagccaaatt
    13981 aactggtatt gttagaatat cagctaggta cattgcaact ggatcgtgat ttaaagaatc
    14041 taagtcaaag gctgttgttg gagctgttgg acctaaaatc aagtcataat ctgcgaaaac
    14101 tttttcaaaa tcttgaataa tcaaactacg aacttgacct gcttttttat aataagcatc
    14161 gtaataccca gaagataaac tgaacgttcc cagcataatg cgacgcttaa cttcgtctcc
    14221 aaaaccttca gatcgtgtat tgacatagat atcatccaag tttttataat tttctgtacg
    14281 gtatccataa cgaataccat caaaacgttg taagtttgaa gaagcttccg atgaagcaac
    14341 aatataataa acagcaacac cgtatttaga atgcggtaaa ctaacttcct caataactgc
    14401 acctagtttt tccagatgtt ttgcggcctt aataatcgtt tctttaactc cttgcgctat
    14461 tccttctccc aaatattctt ttggtaaagc aattttcatt ccttgaatat cttgaccaat
    14521 tttagcagta aaatcgccta cagtacgctc agaagaagtt gaatctctta catcatgccc
    14581 agaaataact gtcaaaagct gtgcattctc ttttactgtt tgagacatcg gtccaatctg
    14641 gtcaagcgaa ctaccgaaag caaacaaacc aaagcgtgat acacgtccgt aagttggctt
    14701 cataccaacg attccattaa atgaagccgg ttggcgaatt gaaccacctg tatcagagcc
    14761 caatgataaa cgtacttgtc ctgaagctac tgctgctgca gaaccacctg atgatcctcc
    14821 cggaacttta ctatggtccc aagcgttatt tgtcttttta aaataagatg tttctgtaga
    14881 gcctcccatg gcaaattcat ccatgtttgc tttaccaatc acaatcatat ctttagcata
    14941 taacttttca acagccgtag catcaaagat tggctcatag ttatagagca ttttagaagc
    15001 agctgttgta agaatcccct ttgttgaaat attatcttta actgctaagg gaatacctga
    15061 cataacatta tcagcatcaa ttcctttatc atcaatttct ttggcttgag ctattgccat
    15121 ctcatcagaa atagtaataa aagatccaac cgcttgttca cgcgcatgaa tatcttccaa
    15181 tgtagctttt gttaattcag ttgctgaaat ttctttttta acgagaaaat catgcaactg
    15241 atcaattgat tgattattaa acgacattag gcatctcctc catcctcaag aattgcaggt
    15301 actttaatat aataatcttg atgctgtggt acatttttaa ataagtcatc acgattatgg
    15361 cctggttgag caatatcttc acgcatcaca gttttgcgat ctgccatggt cgttgttact
    15421 ggtacgcctt ctgtgtcaac ttcatttaag agttcaatca tatcaacaat ctttgacaaa
    15481 cttgacgcaa attctttagt ctcttgatca gaaaagcgca attttgacag atgggcaaca
    15541 tgacgcactt cttcttcaga aattttcatg agttctcctt atctctgtat cattttcgtt
    15601 ttaagatagt ctctactatt atagcatatt aaactctcaa tgttatcaca ttatcccagc
    15661 ttttcataat aaacgcttct tacctatgat aagaagcgtt tactgtttat ttatctattg
    15721 ttatatcatt tctgctttgt ttgattttag cctgtgcagc cgctaattta gcaataggaa
    15781 tcctaaacgg cgaacacgat acataatgta acccctgttg gtaacagaac tcaatagatg
    15841 atggttctcc accatgctct ccacagatac ctattttaag gtttggctta acttcttttc
    15901 ctaacctcac tgcctgctca ataaaccttc ctattccttt ttgatctaaa acttggaaag
    15961 ggtctttttt tagaagtcct ttatcaacgt attctcctaa gaattttcca gcatcatctc
    16021 gtgaaaatcc aaagctcatt tgtgttaaat cattggtccc aaaactaaag aaatccgcaa
    16081 actttgcaat atcatccgct atcatacaag ctcttggtgt ttcaatcatt gtcccaatag
    16141 ggaatgataa gttaattttt ttagatttac tctcctcaac aattgtctct tcaatcagat
    16201 cacgtattat agagatttct tcatgcgctg tcactaaagg aatcataatt tctggctttg
    16261 cctcatatcc ctctttcatt gcaagtatag cgccttgtac aagagctctc acctgcattt
    16321 ggtaaatttc cggataggta atcgcaagtc tacaaccacg atgacctaac atagggttga
    16381 attcttctaa tgtttgaatc cgtttttcaa tagctaaagt agatatcccc atttgtcttg
    16441 ccatagattc tatagactct ttatcatggg gtaagaattc atgtaaagga gggtcaagca
    16501 aacgaatcgt acaagcttta cctttcaata ctttaaaaat ttgataaaaa tcgtctcttt
    16561 ggaaagataa aagcctttct agagccttcc ttcggctcaa tatttcatcc gctaaaatca
    16621 tttctctaac gactggaata cgttcatcgt caaaaaacat atgttccgtg cgacaaagac
    16681 ctattccttc tgctccaaag tccaaggcct tttgagcatc tctcggatta tctgcatttg
    16741 aacacaccat catatctcga ttttcatcta cccatttcat aaatatctca aaatcacgat
    16801 ctacgctagt ctctgccatc ggaacttgtc ctatataaac atttcctgta gcaccatcga
    16861 ttgataacat ctcaccttct tttatcgttt gatgcccaat agaaatttct cgtcttactt
    16921 catcaaccaa taattgacta cagcctgcaa cgcatggttt ccccattcct ctggctacta
    16981 ctgccgcatg ggaagtcatt ccgcctcttg ctgttataat cccgtttgca ctaaccatcc
    17041 cctcgatatc ttcaggtgat gtctcttgtc tgaccaatag cactggattt ccctttttca
    17101 tctctttaac aacatcttct gcatggaaat aaactttccc ataagcagct ccaggagaag
    17161 ctggaagtcc tgtcgtcaaa ataatagctt tctttaaact tttcaagtca aaagaagggt
    17221 gtaataactg atctaattgt ttgggctcga tacgtagtat agcttcttct ttactaatta
    17281 ggccttcatt tacttgatct actgctattt tgatagctgc cttcgctgta cgcttaccgc
    17341 ttctcgtctg aagcatataa agattaccat tttcaattgt aaactccata tcttgcatat
    17401 cgcgataatg agcttctaac ttttgggtaa cgcttacaaa ctcttggtaa ataataggca
    17461 tttgttctgc caattttgat atggattgtg gtgttctgat acctgcaaca acatcttctc
    17521 cttgtgcatt aatcaaatac tcaccaaata aatttgctgc tccagttgat gggtttcgtg
    17581 taaaagcaac tcccgttccg ctgttatttc ccatattccc aaaaaccata gcttggatat
    17641 taacggctgt cccaagcgtc tccggaatat catttaattt acgataaatc ttagctcttg
    17701 gattattcca tgatttaaaa actgcctcaa ttgctaacaa aagctgcctt ttaggatctt
    17761 gcggaaattt ttctcctgct tctttttgat atagttcctt gtaaaattca acaagtctct
    17821 ttaaatctga accagttagt tctgtgtcat cttgataaca tttatctgtc ttaagtcgat
    17881 ctaaaatggt atcaaattta tattttggaa tgcctgtaac cacatctgca aacatttgga
    17941 taaaacgacg ataactgtca taagcaaaac gctcgtcttc tgtctttttc actaaacctt
    18001 ggacactacg atcatttaac cctaaattta aaactgtgtc catcatacca ggcattgaaa
    18061 aaactgatcc tgaccgaact gacaccaaca aaggatcatc aacacttcct aattgcttgt
    18121 tctgttcaac ctccaattgt gccattgctt gatcaatttg actaagaata ctctctcgga
    18181 tatggcaagc gttatcataa taatcattac aagcctgtgt tgtaatagta aaaccttgtg
    18241 gtaccggtaa cccaatactc gtcatttcag ctagattagc ccccttacca cctagcagtt
    18301 ccttcatttc cttacaacct tcatcaaaat gatacacaaa cttagtttcc atcagcgtct
    18361 ccttttacag atttttagct acttgtccat ttgtttttaa gatttctaaa ataatcgttg
    18421 ctgtttcttc gattgcttta tcagatacat taatgattgg gcaacctaaa tttttcataa
    18481 cttcttcagc atacctcgtc tcctccaaaa tccgttctaa actggcataa tttgccgtac
    18541 tagaaagacc taaggacttt aatcgaactt ttcggacttg attaagatga tcaaccgaat
    18601 tagttagtcc gataatcctt ctagaatcaa tcatacgtaa ttctttagga acaggaacct
    18661 ctggaaccaa aggaatatta ataactttaa tatttttatc tgctaaaaac atacttaatg
    18721 gtgttttaga agttcttgaa attcctataa taactaaatc tgcttgtaaa atacctcgcg
    18781 gatctctccc atcatcatat tttactgcaa attctattga ttctactctt ttgaaataat
    18841 cattgtctaa gcgtcgtaaa atccctggtt ctcccaaagg atctataccc gatatccttg
    18901 atattccttg aataacattt gttaataagt caacataagc ataatgttca ctttcacatc
    18961 gtttctgtgc ataggaagcc aaagcaacat caactaaact aaacatcata aatactgtct
    19021 tctggctagc ctcttcaaaa acttgttcta agcgttcctg actgttaata taggaaaaac
    19081 gttgaaaatg ccaatcatca tgtcctggaa attgtgacaa acatgctttc gcaatagctt
    19141 tggctgtttc ccctaaagaa tctgaaataa taaatatagt gagttgatct tccatcagtt
    19201 tcctcctaat aatctcttat ttctaaaaac aaactagcga ggattgtttt agataatttc
    19261 ccgatcactt taaatttttc gggatattgc ttatcatgac gaacgacagg gagactatcc
    19321 acttttctgc tcactaattt atcagctgcc gcaaaaagac tttcattttc taaaacagtt
    19381 gtcacgtgtg gcatacgtgt catcaccatt cctattggca ttttagaaag atctcctccg
    19441 ccaatactgg tttttagtaa atctttacgt gacacgactc cacagagaaa atcatcatca
    19501 tccaagataa aagcacaacc agcatcttcc ataaaaatat gtacaataac atcataaact
    19561 gaatcttttt gatgaactgt cagagggatc cccataattt ctgaaactgt catcttttca
    19621 aaatgacttg tccctattga agcatgatac tgtcctaaat aaaaataacc aaccttaggt
    19681 tttgcatcta tgatacctag catacttaaa accgccaaat cagaccttag tgctgctctt
    19741 gttactccta atttctcagc tattagctca ccagtaatgg gctgaaactg tttgataata
    19801 tcaataattc tttcttgcct ttgatttaat tgaataagat caccttcttt atatattgta
    19861 tactagttat tttatatgtt atattatata tcttttttta ctgaaatcaa gtaaaaaaag
    19921 atactatttt aaaaataatc atactatata tttgctttta gacatatctt ttgttaaact
    19981 ttcttcgaaa acgttttcga atataagtat cggaggttgt aatatgacaa ttaaaaatct
    20041 cactgttgca ggaagtggcg ttctaggtag tcaaatcgcc tttcaagcag cttacaaagg
    20101 aatgtctgtc accatatatg acattaacga tgaagcttta aataagggaa aagaacgcat
    20161 caaaaaatta gctaaagtct atcaatcaga aattgaaaca gcaaaagaag catatagtga
    20221 taaagctaaa agtatcaagt ataataaaaa cttattgcct agcttagacc atatttttct
    20281 ttcaaaagtt gccgattcat tagacttgat tgctgattta ccaaaccaaa ttactttttc
    20341 aaaaaactta gaccaagctg taagtgacgc cgatttagta atcgaagctg tcccagaaac
    20401 agttagtata aaagaagatt tttacaaaca attagctaaa gtggcacctt caaagacaat
    20461 ctttgcaaca aattcttcaa cacttgttcc aagtcagttt gctgatataa caggacggcc
    20521 tgacaaattt ttagcaatgc atttcgctaa caatatttgg caaaataata ttgttgaaat
    20581 tatgggacat aagggaactg acgacgaggt tgtcaaagaa gctttggcat tctcaaaaga
    20641 tattggtatg gtacctttat atattcataa agaacaacct ggctatattc ttaattcaat
    20701 actagttcca ttcttagaga gtgcattagc attgtactat aataaggttt ctgattctga
    20761 aactattgat aaaacctgga agttaggaac aggagcaccg atgggacctt tagaaatttt
    20821 agatattatt ggcatcgata ccgcttataa tatcatgaag aattattcag ataccaccag
    20881 cgatcctaat agtttacatg ctcaccttgc aaaaatgctt aaagaagaat tcattgataa
    20941 aggacgtacc ggtaaagctg ctggacacgg cttttatgat tacgactaaa ctataaaaga
    21001 ggttagataa aaatctaacc tcttttataa ttcaactaaa gaatagttgc accaagaaca
    21061 gttttaaaat gatttaaagc ccattgatga ttactatcat ttaagctggc tacagcagct
    21121 gctgggacct caattttata acctaagtta taagcatcaa ttgctgtgtg taatacacaa
    21181 atatctgtca gtactcctgt caatatcaaa gtatctactc gtctttcacg taggcgaata
    21241 tctaaatcag taccagaaaa ggcagaataa tggcgcttat ctatccaaaa aacacgacta
    21301 tcttccttaa tagtttcata tagagttccc aaaggaccat ataggtttct accagaagtc
    21361 cctttaatat tatgctcagg aaaaagattg gtctctggat gaaaaacatc tccaatatca
    21421 tgattatcaa tcgcaaaaaa aatataatcc cctgaccggt atgctttctc tgtaacgtca
    21481 gctatagctg aagcgataga ttgcgctggt ttacctgccg ttaatttccc atcatctgcc
    21541 acaaaatcat atgtgtagtc tattgagatg agtgcttttg tcatttttct tcttatctcc
    21601 tcttgatttc aaaaaaggaa ctgaaagcta ctcccagtcc catgctatga ttaattatat
    21661 tcttttaatt tgtcaaaaat accttcgtta ataactttga ggtatgttcc cttcataccg
    21721 agtgagcggc tctcaatgat tcccgcagac tccaatttac gaagagcatt tacaataacg
    21781 gaacgtgtaa ttccaatacg gtctgcaata acagatgccg ttaggcgtcc ttcaagtcca
    21841 tctaattctc ctaagatagc agctacagct ttcatttctg aataagatag agtgttgata
    21901 gccattgtca ccgctgtttg tttacgaata ttttcttcta gattttctgt ttgaaggtta
    21961 agtaattgaa taccaacaac ggtgcttgca atttcaacta aaatcaaatc atcgtcgcta
    22021 aattctttat cattacgcca gataataaat gttcccaagc gcattccacc accataaatt
    22081 ggagcgattg ttgtaatacc atcttggaag ttttcctttg tctcaactgg gaaaattgac
    22141 aaatcattat ctactgataa gttagcttct gtatcataaa cacgactagc agatttaaca
    22201 tagtaatctg ggaattgttt tgtttcaaaa aattcttcca cacggtcagt gtttgtttta
    22261 tatttcatgg catagccaag taagttacca ccaccattaa taatacatgc gttacagtca
    22321 ataatatcag ctaattgagc cgccattgta ttatatggta attcggcatc taagctatct
    22381 acggaacgct gtaagatgga tgtaattttt ctagtttttt ctaataaatt cggcatttcc
    22441 ttcatccttt gtatgttttg cgttacaatt atatcaaagc attttagctt ttgcaaatcg
    22501 atatcagtac attttaagaa cttctattgt taatttaatg tttatctaat aaaaattcat
    22561 tctagcgctt gtattggctt aaaaagcgag ccattttttc ttgtagttca gtcaattcat
    22621 caacacgtgg taagtaaaca attctaaaat ggtcagcggt attcatatta aatcctctac
    22681 cgtgtgttag taatactttt tcttgtttta agaaattaag cacaaactct tcatcattat
    22741 caatacggta catatctgta tcaattttag ggaaaagata cagtccagca tttggtttta
    22801 cagcagataa accaggtatt tcattaattg ctttatggat ataattacgt tgctcgtaga
    22861 tacgacctcc tggcaacaac atactatcaa tagattgctg tccgcctaac gatgtttgaa
    22921 taacctgttg agcaagaaca tttgaacaca gcctcatgtt agctaacata ttcaaacctt
    22981 caatataacc tttaacatgt tgtcgcggac cagacaatac catccaacca acgcggaaac
    23041 cacagatgcg atgtgattta gataagccag ataaagtaac tgtaaaaata tcctctgcaa
    23101 tagaagcaat tgggatatgc tccattccat ccataactaa acggtcataa acctcgtctg
    23161 aaaaaataat caagtcattt tgtctagcga tatccacaat ctcttgcaga atttcacgcg
    23221 gatagactgc tccagttgga ttatttggat taatgaggac gattgctttt gttttggatg
    23281 tgatttttga tttgatatca tcaatatcag gataccaatt agcttcctca tcacaaatat
    23341 aatgcacagc atttcctcca gcaaggctaa cacaagctgt ccataacggg taatctggca
    23401 ttggaactag aacctcgtca tcattatcaa gcaaagcttg cattgacata gagattccct
    23461 cactaacacc attaacaatg tatatatcat ccatgtcaac atggatgttt tgtaactggt
    23521 aatactgcat aacagcctta cgagctgaaa aaataccttt cgaatcagag taaccttctg
    23581 attcacgcgc attcgtaatt aaatcacgaa tgacttcatc tggcgcttcg aagccaaagg
    23641 cagctggatt tccagtattt aaccgcaata tcttttcacc atttgccctc atgcggtccg
    23701 cctcttctaa aacagggcct ctaatatcat aagccacatg ttccagcttc attgatttat
    23761 caaatatctt cataaaaata cctcgtatct tccattataa aacaaatatt ctcaaaattg
    23821 aaacatttac tgcggtttat caagcgatta agttaatatt aagctatttc agaaaactaa
    23881 cataaaacgc tttcattttt ctagaaaaaa cgttataata tagctataaa atgaataacc
    23941 aacatatcta acttactcat cactcactcc ttctgataag atggctagtt gagtgcaaga
    24001 ggttatctct tttggttata tttagaggag gacaggctta tgtcacaaaa atatgaacgc
    24061 atccttgtcg ctattgatgg atcaacagaa tctgaacttg cttttgaaaa ggcagttaac
    24121 gttgctcttc gcaatgattc tgaattaatc ttgacacatg ttatagatac acgtgctcta
    24181 caaagctttg cgacatttga tacttatatc tatgaaaaat tagaaaaaga agcaaaagat
    24241 gtccttgagg aatatgaaaa acaagctcgt gaaaaaggag cagataaagt tagacaagtc
    24301 attgaatttg gtaatcccaa aacattactt gcacatgata ttccagaaaa agaaaaagta
    24361 gacttgatta tggttggtgc aacaggtctt aatacctttg aacgtttcct tatcggatct
    24421 tcctcagaat acatccttcg tcacgctaaa gtagatttac tcattgtacg tgaccctaat
    24481 aaaacaatgt aaatttaata cgctcacttt ctaaaatatc ctgagttata ttaactcagg
    24541 atatttttat tttcgattta aggatttctg aatttggcta tctaattcac gtcgaattga
    24601 tggctcagga gcgaaatgtt cttcccagtc gtctggtttt aaaattttat gagtaacagg
    24661 atcaaaatga gctttgccat ctggaaagat tttacccata ttggcctcat gtactgtatc
    24721 aaaaagaggt tttgggtcga ctcccataag aacaaaggag ccataagtca gataaagtaa
    24781 atctgttaag gcatccactt ctcccactaa tggtgtttct ggatggtctt tagaaataac
    24841 cttatctctt gctttatcaa tagctaagtg taaattacgg atagattggt caaatacttc
    24901 ttggttacct tgactagcag catacaagaa ctcaacgatt tcttctactt taaagtccga
    24961 cctaaaacca gcttccttac tgtcatataa gcgtggcgtt ttaatagttt caccatccat
    25021 taatagatgg aaagatttta ctttattgaa attctcgtca cgactactga atgttttttc
    25081 aatctcaaat tggataagtc cataatgggc taatgcctta gagataccat catcattatt
    25141 gtcagcagtt gtataacgtg cattttcttt cacttgctga ctagcgttcc ccatggcaac
    25201 accaatacca acttgagata acatctcaat atcattatca gaatctccaa atgctatcac
    25261 ttctgataaa tcaaaaccat agcattgacc aacacgttca attcccttaa cttttgactg
    25321 cccttcagaa atgacatcca ttgagtaagg actgcttctt gttaatttaa cacgaggaaa
    25381 ttgtttttga attcgctctg attcgccctc tgttgcaact aaaacgacct gataaatagg
    25441 ttctcgtaag ataaccatga ggctatcaat attttgaggt ttaatccgac gaataaaatg
    25501 cttaaagctt cgttcaatcg cttttgccca ttttctcgga acaaggctag atacaatctg
    25561 ccccaaacgg ctagttccta gaccaataat acctgaaccg agtaatcctg aggctgttcc
    25621 taatgaaatt tctcttcttc ttgctccagc ataacgaatc agacgataga cagttgtctt
    25681 agataattga ttggtataaa taatttcact tctactatag atatattgac cattataggt
    25741 tacagcaaaa tctaaaccaa ggttttctaa aaaaggttgt acaaaactag gacctcgctc
    25801 cgttgctaaa ccgactagta ttccttggtc tttcaaattt cgaattgctt taattgttga
    25861 cttttgtaca ttcttacgat cattaagaag tgtaccatca atatcaaaaa atactgcctt
    25921 aattgccact gggtcaacat cccctctttt ttatgataaa ataaattata tcacaacttg
    25981 aaagaagtat aaaatcaatg aaaaaaatcc ttgttctaca cacaggtgga acgatttcca
    26041 tgaatgctaa cgagaaggga caggtcatgt ccagcgccga taacccaatg aaatacgttg
    26101 atttatcatt agatgacctt gatttgactg ttgtagattt tctaaatctt cccagtcctc
    26161 aaatcacccc tcatcatatg cttgatatct atcactatct gaagcaacat gccagtaatt
    26221 ttgatggagt tgtcattacg catgggactg acactttaga agaaacagca tattttttag
    26281 acacaatgat actccctaaa atcccaatca tcattaccgg tgctatgaga agcacaaatg
    26341 aacttggtag cgatggcgtt tataattacc tatcagccct ccgtgtcgct aatagtacta
    26401 aagctgccga caagggggtg ctagttgtca tgaatgatga gatacatgct gctaaatatg
    26461 tcacaaaaac acatacaaca aacgtttcga cattccaaac ccctacacat ggcccccttg
    26521 ggattattat gaaacaagac ctgcttttct ttaaagcaac tgaagagaga gttcgttttg
    26581 atttagataa aattacagga actgttccta tcgtgaaagc atatgctggc atgggtgata
    26641 gtggtatcat tagcttttta aattctcaaa atattagcgg attagttatt gaagcactcg
    26701 gtgcagggaa tatgccccca aaagctgcac aagaaatcga agaattaatt gagcaaggtg
    26761 tccctgttgt tctagtgtct cgctgtttta atggtattgc tgagccagtt tacggatatg
    26821 aaggtggcgg agcaaaatta caagaatctg gtgtcatgtt tgttaaagaa ctaaacgctc
    26881 ccaaagccag actcaaatta ctcattgcac tcaatgcagg tctcacaggg caaaatctaa
    26941 aagactatat tgaaggttga taaaaaactc tagaaatatt tctagagttt tttattaatt
    27001 ttcagtttgt aatactctct caacactgct aataggcatt tcttgattcg ttattaattg
    27061 aaatgcttct gctccttgat aaatcagcat ccctagacca ttataagcat gtttcactcc
    27121 attttgtcta acaaatcgta acaatgctgt ttctttaggc ttgtaaacca agtcgacaac
    27181 tactaaattc ggtgttaaaa tttctggatc gttaattaaa ctataattat ctaatggcct
    27241 cattcctaaa ctagtagcat caatataaaa atgactagtt ctaatagcgt ctttaaatgc
    27301 tgtcttattt tctagataat caacgactac ctttatttga aactgttttt taattttatc
    27361 tgataagtca atgaccttat cgtaatttga gctgttacga ttaaataatc taatttccgc
    27421 aactccctcc atagctgctt gaactgcaac tgctttacct gaaccaccaa taccagctat
    27481 tgtaattatt ttatttttag cactgaaacc ttgagctgct aaagctttaa aacaaccaat
    27541 gccatctgtc atatggccta ctaaacgtcc ggttccacct tgattaacga tagtatttac
    27601 agcacccact aatttagctt gaggagataa atcatctagc aaagggataa cactctgttt
    27661 aaatggcatt gaaacattaa caccacgaat actcaatgcc ctgacacctc gaacagcttc
    27721 tgttaattta ccctcttcta cttcaaatgt cagataggca taattcatgt ttttttcttg
    27781 aaaagaggta ttccacatta acggggatag agagtggcgt gcaggattgg caattagacc
    27841 agttagtaaa gtttccccat ttaatgaatt cattgtataa ccttctttct taacaattta
    27901 tttcagttca ttttaccaaa aactgactca attaaaaaga gagtatgtaa tttatactct
    27961 ctcccttagt ttaaggcaat atattacctg cactacggta aatatcatac tattctttac
    28021 gggttaaagc gatatcatta gccttatcaa tcgcctttaa acgagatgga ttcattgaac
    28081 caacaatcgc ctgcattttg gcaggatgac gtaaaatcca tgcaatcaca atagcttcgc
    28141 tgctaacatt gtagtttttt gccagtttag ctatggtttc atttaattcc ttataatccg
    28201 gatgattaac aaataaacca cgagataaat caatttgaaa tggagaccaa gcttgaatgg
    28261 taactctttt aagacgacaa taatcaacaa tgccatcatc atgttcaatt gaagctttat
    28321 ttaacatgtt aacatttaac cctgcatcaa acattggtgt atgtgctggc gataactgaa
    28381 gttgattcac tgctaagggc tcgtcaaggt aggattgtaa caactccatt tgaaaccggt
    28441 tttgattgct aacaccaaag tgttttactt taccttctgc cctcagtttg gtaaatgctt
    28501 ctgctacttc ttcaggctca actaatacat ctggacgatg aagaatgaga aaatctaaat
    28561 attctgtccc taatctttct aaaataccat ctactgatga taaaatatac tctttagaaa
    28621 aatcaaagta accttctcgt atcccacatt tagattgaat aatcattttg tcacgattga
    28681 catttaaatg tttgattgca tctcgaaagc gtaattcaga tagccctccc ccataaatat
    28741 cagcatgatc aaaaaaatta atacccaaat ctaatgctgt tccaactact tcttcagctt
    28801 gttttccttt caagtcactc attctcatac aacctaaagc aattcgagtt gcttgaatgc
    28861 ctgtttgtcc aatgttttgt aacatatgca ccccattcta atctgtttgt attaacagaa
    28921 agattaatca attcattata acaaaatttc tatgaaaagg cttactatta caaatgcaaa
    28981 aagctgagta aatttaactc aacttttttt ctgatactca ttattgacta aataagtcgg
    29041 taagaaaaat ctattctgaa aagtactatt ataaattaca gtaatcatta ttgtcataac
    29101 atacctccac atctgtgata ggaatatcat atgacagcaa gcttaaactg agcttagcac
    29161 agccttaata gaaacttaat caaaatctga atgttggcgt aaattatcta gaataagtgc
    29221 ccattctgta ttttctttcc aattattatc tttgacaata tcactagcaa cacgtctggc
    29281 ttcttctaaa ataggataat cctcaacgat atcagctact tgaaattctg gaattccaga
    29341 ctgacgagta ccaaagattt caccagaacc acgcattttt aaatccgact cagctaaaac
    29401 gaaaccatct gtcgtttctg tcatgattgt cattcgtttt ttcccagaat cagttttggg
    29461 attagccact aaaacagcgt atgattgttt atatccacga ccaacacgcc cacgaagttg
    29521 atgtagctgg cttaatccaa aacgatcggc atccataata atcatgatcg ttgcatttgg
    29581 gacatttacc cctacttcaa taactgttgt agatactaaa atatgacttt ttttatcctt
    29641 gaaatcttgc attatagcat ctttttcatc atttttcata cgtccatgta caagagcaac
    29701 cttagcaatt ccttcaaaat aagtagataa ttcagcatgc aatgctactg cattctttaa
    29761 atctaaagct tctgactctt caatcaacgg tgaaatgaca tacacttgag catctttttg
    29821 caattcacct tttacccatt ccaacacagt acctagctgc tcgtgtttca cccagcgtgt
    29881 aataataggt ttacgaccgg caggtaattc atcaataata gaaacatcca tttctccaaa
    29941 agctgtgatt gctagagttc ggggaattgg ggtggctgtc atcattaaaa catcaggatt
    30001 ttctcccttt tcacggaaaa ttctacgctg tttaacacca aaacgatgtt gctcgtctgt
    30061 aattacaagc cccagtttat ggtactgtac cgagtcctgg ataagagcat gagttcctac
    30121 aatcatatca accgagccat ttgcaatagc tgctaaaacc gtacgcttaa cagctgcttt
    30181 catacccgaa gttaatatag cgattgataa atctggaaat aactcttgca gactaatgta
    30241 gtgttgttca gctaggattt ccgttggaac catcaaagcc gattgaaaac ctgctgtata
    30301 agctgcatac attgatagac cagcaatgac tgtctttcct gatcctacat ctccttgcaa
    30361 taaacgattc atatgagctc ccgatgacat atcagataat atttcatcta aagagcgctt
    30421 ttgagcattc gttagaataa aaggtaaaga tgaaatcttt gtctccatag catgtttact
    30481 ataaagaata gacaaaccat ttgtttcaga tttattttcg gctttcaaaa cttgaaggtt
    30541 catttgaaag taaaataatt cttcaaattt aatccgacgg agcgcttgct tatactctgt
    30601 gatatctttt gggaaatgca tagctaaaca agcctgacta cgacccatca atcggtattt
    30661 tcccaataaa gtagctggta aattttcctt caattccaaa tgcgcattga tttcaaaagc
    30721 tgacttaata gctttaatca aagtagactg tgaaattccc tgagatacgc gataaacagg
    30781 ttgcatgtca tcttcaactt gagctaaaac cttcatccca gtaatagctg atttagttgc
    30841 atcccattta ccaaagacag ctacctcttg accaagttct attttatcag ctaaataggg
    30901 ttgattaaaa aaactaacat ttaagacagc ttccccttga cgcaatttga aacttaaacg
    30961 gttacgttta aaaccataat attgtacatt agctggagta acgactaagc ctgtaatgac
    31021 tgctttttca ccatccacta aatcaaaaac agattttgat ttaaaatctt catagcgaaa
    31081 cggataataa agaagtaaat cttctactgt ataaatatct aatttctgaa atttttctgc
    31141 tgattttggt ccaaatccct taagattaga aattggtgat tgtaatagca taagattctc
    31201 cttctaatat cttttattat actaaaagat caccaaaaag gcgcctcaag agcgccttta
    31261 ttatgtctaa ttatttatgg ccaataacat aagttaattg tgcagcttgt gctgaactaa
    31321 aagtacggta agagacaata ccacgtccca ttgcattaga ttctgaaatg agaactgaac
    31381 catctttttt aacttcttca acaatagcta cgtgaccgta agtgccatca gcaccagctt
    31441 gtcctggtga aaatgataca gcatagccta cttttggtga atgtgttgtt tcaaagccag
    31501 ccttatgttg ccaatctcca ccattaccca taaatggatc aaatgtataa cctaactctt
    31561 tagcgcggtt aaagacatac catgtacatt caccccatgg ataagttgat gcaatatagc
    31621 tagctgtgtt aaccgctgtt gataagctaa aaccacttgg taaatctgct aaacttgctg
    31681 tcgataaagc tttcaaggca gcaggaattt ctggtgtccc tccatgttga tcaatccagc
    31741 ggtctaaatc ttccataatc tgggcacgac gtttaccagt tccagagtta tctgtataat
    31801 aaacaccacc ctcagtaact gtattaagtt gtccagaaga taatttctgt gctttcaaat
    31861 cttggatttc aaagctagag ttgttgtttt taataattgt attttttgtc aacaacataa
    31921 ttgcttcttc atcattataa gcactagcat ggctagagtc atgatcaaag gcaccataac
    31981 caaacatatt agcacctggc atcttagcca ctccttgagt tcccaatgaa ctttcaagga
    32041 cagcaatagc tacaatagca cggacatcta aaccactatc tttttcccat tgtaataact
    32101 tagcaccatt gatacgatta gaatcatatt gaatccctaa agattttaag tacccatcta
    32161 attgttcagc tttgataccg taacgatgtg acaataaata gtgagagtag gcatcctgac
    32221 cagaccagtg ttctacgtag aatggcgttg ctgtcactgg tgaaatcggt gttgataata
    32281 agttatcatc aaaggataaa gcaggaacag aagcattctg atttgtctga acactttcta
    32341 tctgagacgg agcgggagtt attttaggct ctactaatgg taaggtaggc tcaggtttta
    32401 attcagtctt tgttgattga cctggcttac tgtctgcttg actactgttt gtagtattag
    32461 tgctaggagt agatgctgtt gaagcagaat cagatgtttt agcatcttcg ttggaagaag
    32521 attgatgtga tgaacttgat gatgattcat tgcttgatga tgtctctgtc gtaccaatct
    32581 ctgttttttc tgttgagata cttccttcgg ctgaaggtga agcagtttct gaatctgtag
    32641 gttgtgtttt ttcctgtttc tctgcttcag aagaactttc tgttgatgct gttgccattg
    32701 tcaggcttgt ttctggatta atagtttctg ctaaggtcac taaaggtgat gatagcgata
    32761 agactaaagt ggataacaac aaagttgccg ttactttttg ttttgacata tactaatgat
    32821 tcccttctat aaaaattgtc actgattagt ctaacatatt tttaaatttt ttaaaacagt
    32881 taactactgt gtcatttgtt tttaataata tctcggaata cgatcgctta aaagacaaag
    32941 tacttcataa ttaatcgtct gtcttttttg agcgacgtcc gtagttgtaa tgttacaact
    33001 accttgttga ccaatcaagg ttactttagt tccgatagta tatttttgag gtaaacggat
    33061 agtcatttga tccatcgaga cacgcccaat aatttcacaa agttctccat ttactataac
    33121 tgaaaatcct tgcatatctc gtgtccatcc atcagcgtag ccaatcggta ctgttccaac
    33181 aaattcgtct ccagtaactt gataggttgc gccatagcca acttgagaac catcgtgtag
    33241 ttgtttaaca tgtactagct cagactctaa acttaaagca gggtttatag ggtaaggtaa
    33301 gtctaaatca gttccacttg gatttaagcc gtacataaca acacccaatc taacggcatt
    33361 aaaaattgtc tcactatgcc aaaggcttgt agcagaatta ctagcatgaa caaggtccgg
    33421 acaattatct aatcctgaaa taaagtcttt aaaaaaagta agttgttgat tgaatttaca
    33481 gttatcggct tcatcagctg ttgcaaaatg ggtgaaaatc cccttgacag aggcaccact
    33541 ttcccccaat tcagaaatta gcttatttcc ttcatctaac tggcgaacac ctatacgtcc
    33601 cattccacta tcaactttga tatgtacttc tagaccagat aaatctactg ctgatgtctg
    33661 acataaacgt aaccactcca agctagcaac tgttagagta atattttcat ttttggctag
    33721 tataacctgc tcaggcataa caacacctaa taccaatatc atttttacaa ttcctgcttg
    33781 acgtagctca atagcctcat ctaaattgga tacgcaaaaa ccatctacaa tagactcaat
    33841 gtattttgaa acttcaacag caccatgccc ataagcatta gccttaacta cagcaaatgt
    33901 ttttttatca cttggaatgt gctcttgtac agatttaacg ttatttgcaa tcgcctctaa
    33961 atctatcaag gctctggttg gtctatgata acttgaaatc atttttcttc ctccaaaata
    34021 acactagctt gtgcataatt accactatga gatattgaaa caaaagattt tccattaaac
    34081 ggttcttttg taatcaaagg cgctccttta tcatccgata aaatttcgat atcatgaaaa
    34141 ttaactttcc caattcctgt tccaagtgct ttgctataag cctcttttgc tgcccatcgc
    34201 cctgttaaaa aagacatctg acgcttggga ttggaaattc ctttaaaaag aagcaattct
    34261 tgttcggtca aaacgcgttc tgcaaaacgt tgattacgct catatgcttt agtaatcgcc
    34321 tctatctctt gtaaatcaat tccgtgtcca acaatcatgt gtaactccta taagttcgat
    34381 acttacaaac tattgtaaat atccctaatt agcatttctg ttttatccca acctagacat
    34441 ggatctgtga tagacttacc aaaaacatct ggtttatctt gtcttccatc ttctaaataa
    34501 gattctatca aaaaaccacg aacatagttt ctaattttag tatgccactg tcggtctgca
    34561 agtactgatt tcacaatgcg aatttgttct aaaaattgct tacctgaatt gtcatgattt
    34621 gtatcaacaa caataaaggg attttgcaaa tcagtctctt cataatgagt aatagtttct
    34681 aaaaggtttt cataatgata attagggata cttcgatagt cggcattatg atatcctctc
    34741 aaaataacat gtgacagcaa attaccatct gttctaactt gcttattttg atagaataat
    34801 tcctgttgat tttgtgctgc atagaccgca ttaaacatga ctcttaaatt accagacgtt
    34861 ggatttttca tacctactgg tgcatctata cctgaagata taaaacggtg tccctgatct
    34921 tccactgaac gcgcaccaat agcatagtaa gaaactaaat catccatcac tgataacatc
    34981 tctggataaa gtaattcatc agctgtcagg agtccagtct ctcgaatgat gtcatagtgc
    35041 atcttacgtg cttgaaaaaa aactcctagt ttgtcagaat ggtgaactaa gcctttataa
    35101 ccatcaccat tcgttcttgg cttagcggta taaactctca taaccaaaaa cattttatct
    35161 ttaaaggcag cttccaattt agctaaacgc tttgcataag ttagcaccgc ctttggatta
    35221 tccgcagaac aaggacctac tattactaga aaccgctgat cctcaccttt tatgatattt
    35281 tttaactctt cttctttggc taattttttt tggctaactt ctagatctaa gctagttttc
    35341 tgcttcaata tttcaatatt aattttatca cttaactgat gaaatcccat gttttttcct
    35401 aatacaacct aagtctatta ataagctctg ctatacaata tagtcgtcat actattagac
    35461 aagtaatgat aaaacagtct tctcagactg ttttattttt attttaaaat cattgttatt
    35521 gtaaacatct cagaccatga caatttttaa acttcatgcc agagccacaa ggacatttat
    35581 cattacgttt aatcccttgg tattctggtg attcattatt cataggtaca tgttgtgccg
    35641 agatattttg ctctgctgtt gtcgtagcat gttgagaagc acgttcacgc tcttgttcat
    35701 gaatttgagc tttcatcaat gtacgtgtca catcaaactc aatcgaacca atcatatctt
    35761 ggaacatacg gaaaccttct gattgatact caacgattgg gttattttga gcataaccac
    35821 gaagtccaac ggagttacgt aattgatcta aggcatcaat atgctctgtc cattgattat
    35881 ccactaccat caagattaag actttttgga attcgataat agcttctgga tcttttaatt
    35941 tagcaatttg ttgctcataa acagctaagg cacgttcata aagaagttct ttaatttcag
    36001 cctcttttag accacgaagt tctgaaactg tgatagcttc ttcatctaat aacgccgatc
    36061 ttgcaaaatt aacaatagct tctgctgcag tatttttatc gcttctgcta tgtgcatcaa
    36121 ctgctctttt aatcgtacgt ttaatcatcc ctttgagctc tgatcctaaa tcacgctcag
    36181 cagtaatcac ttctctacgg ttagcataaa taatctcacg ttgttcacgc ataacatcat
    36241 cgtattgcaa gacttgttta cgtgtgtcat agttattacc ttcaacgcgg cgttgagctg
    36301 attcaacttg tcgtgtcaac atttttgatt taataactgt atcatcttca gctaaattca
    36361 ttcgctcaag aaccacttta atacgatctg taccaaagcg acgcatcaaa tcatcttcta
    36421 acgaaagata gaattgtgat tcacctggat ctccttgacg acccgaacga ccacgaagct
    36481 ggttatcaat acgacggctt tcatgacgct ctgtaccaat aacacataaa ccaccaagtt
    36541 cacgaacccc ttcaccaagc ttgatatcag taccacgacc agccatgttg gttgcgattg
    36601 taacagcacc acgttgccca gcattcataa tgatttgagc ttctttaaag tgatttttag
    36661 cattcaaaac ttcatgtggt actccagcag caactaattt tctagaaatt aagtctgacg
    36721 tttcaacagc tactgtacca accagtacag gttgtccttg ttcataacgt tctttgacat
    36781 ctgctaccac cgctctaaat ttagagtcaa gagttggata aagtagatct gaatgatcta
    36841 tacgttgaac aggacgattt gttgggatag gaataacgcg catattataa atttcacgaa
    36901 actcttcttc ctcagtctta ccagtaccag tcattcctgc taatttatgg tacatacgga
    36961 acatattttg gtaagtaatt gaagcgctcg tttttgattc ttcttggatt ggcactgact
    37021 cttttgcttc aatagcttgg tgtaagccat ctgagaaacg acgtccttcc atcgtacgac
    37081 cggtaaattg gtccacaatt aaaatctctt gttcttcact aaccacataa tcaatattta
    37141 acaacataat atagttagct cgaagagcgt tatcaatgta gtgagttaag gcaacatttt
    37201 cgagatcata caagttattt agatggaaat agttttcagc cttgtcaata cctgtatctg
    37261 ataaaccaat tgtttttgtt ggaacatcaa tgatataatc atcgctatta agcgttttta
    37321 caaacatgtc cgcacgtgta tacagttggt tcatctcaga gcttacagga cctgaaacaa
    37381 tcaatggcgt cctagcttca tcgattaata cagagtcaac ctcatcaacc aaagcatagt
    37441 tgagtgggcg ttgcaccata tcttcttggc gaacaaccat attgtctcga aggtagtcaa
    37501 atccgacttc tgcgttagtt gagtatgtga tatcacaatt gtaagcttcg cgtttttcaa
    37561 atggtgactt agctgctaag ttaatcccta cagataatcc aagccaacta taaacctcac
    37621 ccatttcggt cgcatcacgt gttgataagt actcattaac agtaataaca tgaacaccta
    37681 atcctgagat agcatttaga taaacaggca ttgtcgctgt taaagttttt ccctcaccag
    37741 tacgcatttc tggaatatca ccatggtgaa gaacgatacc acccataatt tggacgtggt
    37801 atgggtataa acctaataca cgtttagaag cttcacgaac taccgcaaac gcttccggta
    37861 acaattgatc taaagtttca ccattttgat aacgttcttt aaattctgga gtttttgctt
    37921 gtaaagcttc atctgataac gtagccatat gatctgcgta agagtcaact ttcttagcta
    37981 ttttatctaa tttttttaat tcacccttgt cattttcaat aacagtgcgt agaatatttg
    38041 ccattctaat tcctttcgat actcttatca ccacatttta ccataaataa tgttgtcttt
    38101 caataaatgg tcgttttaat cgagattaaa acaagtggta tccccactct cattattttt
    38161 taacatgact tgctataatt tctaactgtc catcaatatc ccatttgaca acatcatttg
    38221 gcaagataaa gtggtcacct tttttaagtg tataaacttt gttatctact gtaatgtgac
    38281 ccgctccgtc taagacacta accaaaaggt agggtgcaaa ttgtttaaag ttggtaacac
    38341 cagaaatttc ccatttataa actgtaaaga aatcatttga aactaataaa gttgaagata
    38401 gatgttttaa cttcatattg gcaggaacag tattggcagg tttaccaatc gtgagaacat
    38461 ctattgattg ttcaatgcgt aaatcacgta attttcctga agcatctgga cgatcaaaat
    38521 cataaacacg ataagtagta tcactagatt gctgtgtttc taaaattaat atccctttac
    38581 ctatagcatg cattgtccca cttggaacgt agtaaaaatc ccctgattta acaggaattc
    38641 gggttaaaag atgttcccaa tctccagatt caatcatttg gcgaagttct tctttagttt
    38701 tagcattatg accatagata atttcagatc cttcatcagc agaaataata taccaacatt
    38761 ctgttttacc aagttcacct tcatgtttaa gagcataagc atcatccgga tgaacctgta
    38821 cactaagcca gtcattagca tctaagattt tagttagtaa aggaaagaca gtatcatcag
    38881 ggttgccaaa taagcttttt tcaccttgat acagcttatc taaaaaacaa cctttaaagc
    38941 gaccattctt aactcttgag acaccgttag gatgagctga aatagcccaa tattctcctg
    39001 ttgtttcact tggaatatca tagccaaatt catcccttaa ctttgttcca ccccagattt
    39061 tatcgtgcat gcttgcttct aaaaataatg gttccgacat aagaatcccc tatcttttgt
    39121 aaaattcaag cctattatag cattttatcc atcatatcgc ttactttttt taatctttta
    39181 tttgctaact aaactaagta gtggcttaag ggttttatta accttttgat attttctttg
    39241 ctaaagcaaa atttcctaac gttgcagaac cattttcttc tatagctggt gtcacaatat
    39301 aatcagacaa atcgggaact ggtaaatacc catttaataa agttgcaaat gtctggcgaa
    39361 cacgtcttaa catgtgctct tgtgccatga cgcctccacc aaatacaatg acttgtggac
    39421 gatataatac agttgcttgt attgcagcct gagcaatata ataggcttga acatcccaaa
    39481 ccattgaatt ttcttcaatc aattcaccac gaataccagt cctagcttct agagttggac
    39541 cagatgccaa accttctaaa cagctattat gaaacggaca gataccttta aaatcatttg
    39601 cttggtcctg cggatgcatc gccatataag tatgacctgc ttcagtatga ccagttcctc
    39661 ctataaattc acccttttgt atagctcctg caccaattcc cgtaccaatg gtgtaataaa
    39721 caagtgaatc aatattgtta cgagctataa cttcaccata ggcagagcta ttaacatctg
    39781 ttgtgaaata aaatggaaca ttgaaatctt tagaaattaa gccgagtaaa tcaacattag
    39841 cccaatgtaa ctttggggtc gttgtgatat aaccgtatgt ctttgatttt ttatcaatat
    39901 caatgggacc aaaagaacca atagcaactg cctctaactt cttttcaaat cttttaaaga
    39961 aatcaacagt ctttttaatc gtttcttgag gcgtcgttgt tggaaattgc attttttcta
    40021 caacttttaa ttcctcatca ccaactgcac aaacaaactt tgtcccacct gcttcaatac
    40081 taccatataa ctttgtcatc ttttttccta tctataatct ctatctcaaa gacaaaggtt
    40141 gggataatta tcccaacctt taatcctcac ttaacatcaa taatcatgtc acctgtagta
    40201 acaacaccct cttttaccaa tggtataaca tcagcaaaat cagctgtatt agtcacaata
    40261 atcatagctg tattatccag acctgcttct gcaatcttgg ctgaatcaaa ggtgccaaga
    40321 atatcacctt ttttcacttt ttggtctgcc ttgactttag aagtaaaccc tgtgccattc
    40381 atggtgacag tatcaatacc tatatgaatc aaaatttctg cacctttatc tgatttgata
    40441 ccgtaagcat gacctgtttc aaaagcaatt tggacaaaac catcaacagg tgagtaaatt
    40501 gtattacctc ttggtttaat agcaaggcca tctcccataa tacctgatga aaaaactggg
    40561 tcacttactg tatcaagagc aataacctgt ccatcaagag ggctaacaat cgtttctgcc
    40621 cttaattggc tagtttgagt aattgttgga tcgtcatctg tttcttcaac caaaggacta
    40681 acttcagcta ctgctttttc atctttatct tcatatccaa acatataagt taatacaaaa
    40741 gccaaagacg tagttccaat taccataata aggtacttaa caatttgacc atttaaataa
    40801 agtaatgttc ctgggataat ggtaatacca aaaccagttc ctgctaattt caatattgaa
    40861 gctaaccagc cacctgctgc tcctgcaatt aatcccataa taaatggctt accaaaacgt
    40921 aagtttactc caaatatagc cggttctgta ataccaagtc cagctgaaag cgctgccgga
    40981 aaagcaaggg cttttaattt tttatttttt gttttaacac caactgccaa cgtggcacct
    41041 gcttgtgctg tcatagcagc tgtaataata gcattaaatg gatctttccc atcagcagca
    41101 attaactgag cttcaagtaa attaaagata tgatgaacac ctgtaacaac gattatctga
    41161 tgaacaccac ctaaaataag acctgatagg ccaagaggta aattaaggac aaattttgtc
    41221 cctgctaaaa catagttttc aactgaatgg aaaataggac caatgacaaa aagtgctaaa
    41281 attgacataa ctgtaaaagt taggaacggc actaatagta aatcaagaac atctgggatt
    41341 ttcttatgca accatttttc aagtttagca ccaagtaaac ctacaaagaa tgccggcaat
    41401 actgaatttt gataaccaac tactggaata aagccgaaaa attttatggg atgggcatca
    41461 cctgaggcca ctgcccaagc gtttggaagg gcagagttga ccatcatcaa tcctaatacg
    41521 agaccgataa caggatttcc accaaaaact ctaaaagctg accaagaaat caaggcagga
    41581 aaaaaagcaa aggccgtgtc agtcaaaacg acagtatagg tataaaaatt agaacttgaa
    41641 aatgcttttg aagtcgtccc aaataaagct aaaactgtat cattattaat cgcaccgcga
    41701 atccccataa ataaaccagt agcaacaatg gctggcaaca atggaacaaa aacatcaccg
    41761 aaagtacgaa tagctcgttg gaaagcattc ccttgtttag ccgcttctgc cttttgatca
    41821 gaggttgaca aagttggcaa accttgagcc actacttcat cataaatctt attaacagta
    41881 ccagttccaa aaataatttg atattgacct gaattgaaaa atgctccctg aactttttcg
    41941 atattttcaa cagtattttt atcaatgact gtctcatctt ttaccataat gcgaagacga
    42001 gtggcacagt gtgctacgct acgcacatta ctagcaccac caatcgcatt aatgacttgt
    42061 ttagcaattt ctgtatttga agccatctat aaatctcctt tgacattttg aaagcgtttc
    42121 attttattta ctctaatatt ctagcatgtc tcataaatat atgtcaagcg tttgacataa
    42181 aataatattt tttgatgtta agagttgatt tcaaaagaga aaacgtttac tatatagtta
    42241 ttagatatta agacagtcaa ggagtatcaa atgaatttac ccactgaaat ccgctatcgt
    42301 ccttatgatg aatggactga agaagataag gaaaatattt taaagaatgt ctcaaaatcc
    42361 ccttggagag caacttatca cttagaagct aaaacagggc ttttaaatga cccaaatggc
    42421 ttctcttact ttaatggaaa attccatctt ttttatcaaa attggccctt tggagctgca
    42481 catggtttaa aacaatgggt acatacagaa tcagatgatt tagttcattt caaagaaaca
    42541 ggaatcaagc ttaaacctga ccatgtcaat gatagccatg gtgcatactc cggttctgca
    42601 ttagccattg acgataagct ttttcttttt tatactggta atgtccgtga catgaaatgg
    42661 aaccgtgacc cccgccaaat tggagcatgg atgacaagtg atggcaagat tacaaaattt
    42721 gataaagtcc ttatttcaca accaaatgat gtcactgagc attttcgtga tcctcagatt
    42781 ttcaattatg ataatcaatt ctatgctgtt atcggggcgc aaaatagtaa aaaatgcgga
    42841 ttcattaaat tatataaagc acttaataat gacatccatc attgggagtt tgtgggagat
    42901 ttagattttg gtggtactgg atctgagtat atgattgaat gtccaaatat tatttttgtc
    42961 aaaggaaaac ctgtattact ttatagtcct caaggactag ataaaaacga attggactac
    43021 caaaacattt atccaaatac ctacaaaatc ggacaatatt tcgatgctaa ctcatctaag
    43081 atagttgaac catcccctat ctataattta gattatggtt ttgaagccta tgctactcaa
    43141 ggatttaata cttctgatgg tcgtgccttt atcgtgagtt ggataggctt gccagatatt
    43201 gattatccta gcgatcaatt tgattatcaa ggagctatga gtttagttaa agagttaagc
    43261 ataaaaaatg ggaaccttta tcaatatccc gttcctgcta tgaaaaatct tcgtcaacat
    43321 caagcagaat ttaaaacaca acttcaaact aacaacacct atgaattaga attactagtt
    43381 cctagaaacg atctttcttc atttgttctc tttgctaatc cgaaaggaca aggactatcc
    43441 attactattg atactgcaaa ggggaaagtt atcgttgacc gctcacaagc cggacagcag
    43501 tacgccacag aatttggtac tagtcgccag tgtgacattc caaaagatgc tactagtatc
    43561 aatattttta tcgataagtc catctttgaa atctttataa ataaaggaga aaaagtattt
    43621 acgggacgag tctttccaga tgcagaacaa tcaggaattc aactaaaaga aggacacgtt
    43681 catggtaaat attttgaatt aaaatattaa tatggttgca aaattaacag atgttgctgc
    43741 acttgccggt gttagcccaa cgactgtatc acgcgttatt aacaaaaaag gctatttatc
    43801 tcaaaaaaca gtcaccaaag tcaatgaagc catgagaacc cttggttata aacctaataa
    43861 tttagcacgt agcttacagg gaaagtctgc taaattaatt ggactcattt tccccaacat
    43921 ccgtaacatt ttttacgctg aattaattga acaccttgaa attgagttgt ttaagcatgg
    43981 ttataaaact atcttatgta atagtgaaaa ggatcctatc aaagaaaaag aatacctcga
    44041 aatgctagga gcaaatcaag tagacggtat catttcaagt agtcacaacc taggaattga
    44101 tgattacgag aaagttgagg ctcccatagt agcattcgat agaaatttag ctccacacat
    44161 cccgatcatc tcatctgata atttctttgg aggaaaaatg gctgctcaaa cccttaaaaa
    44221 acatggctgt caaaagatga ttatgattac agggaatgat aattcagatt ctcctactgg
    44281 acttaggcgg ctaggttttt cctatgaatc aaaggaatcc aaagttatca cagtcacaaa
    44341 tgggctatct aacatgagac gtgagatgga attaaaatca attatctcga ctcacaaacc
    44401 agatggtatt tttacatcag acgacttaac tgctttatta gtaatcaaac ttatcagcca
    44461 actagggttg tccatcccag aagatatcaa agtgattgga tatgatggta caagtttcat
    44521 tcaagattac gtcccacatc taaccactat taaacaacct attcgagaaa ttgcgcaatt
    44581 gatggttgaa atactcctag ctaagattga ggggcaaaag acaaataagg attatatttt
    44641 accagtcagt ctcatccctg g
//
LOCUS       DATROD010000007        42405 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.007, whole genome shotgun sequence.
ACCESSION   DATROD010000007 DATROD010000000
VERSION     DATROD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42405
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.007"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..261
                     /locus_tag="VBC74_000371"
     CDS             <1..261
                     /locus_tag="VBC74_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6941349.1"
                     /translation="RHQDCQKRQFNKMPYEEISEVSHYVKSKGLVLDDYIAYRDTLTK
                     VEETLSDIDKEKFEKLINGERFAGKKKFIRNIQPFFNAFKAD"
     gene            complement(440..1537)
                     /locus_tag="VBC74_000372"
     CDS             complement(440..1537)
                     /locus_tag="VBC74_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6941350.1"
                     /translation="MIEKYTKKDGTTAYRLRAYLGVDPMTGKQVRTTRQGFKTEREAK
                     RAEVKLIDDFQRQGAWKSNDKTTFDDVAKLWFEQYRNTVKPSTFLVNQNYYKTILKPH
                     LGQLQMTKITVMICQKFVNCLSRYSGYRLYLSLANRIFKFAVNLGIIDNNPMSKTLRS
                     KCTYKNMDTLTKKYYTKEELNAFLRIVEAEETLEMRLIYRLLSYGGFRIGELIALKDT
                     DFDFRNNTISITKTIAYTKEGWAVQSPKTKKSNRTISMDAETMTLAKLYIKQSIKPLH
                     GSFKLFNFASDTVRKRLDRFILKHGLKRITPHGFRHTHASLLFEAGIPAKIAQERLGH
                     AKIAITMDLYTHLSKKSKDNVADKLAELVAI"
     gene            complement(1710..2324)
                     /locus_tag="VBC74_000373"
     CDS             complement(1710..2324)
                     /locus_tag="VBC74_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941351.1"
                     /translation="MKKPFYKKWYFWTHAILLLVLGFSFFVIYRLAETNIVNEKKIAK
                     LEKTQENKTTSGIRKTISDFTSRFDEELSVRAIKFYLNKDQVVSSFGDEVKLGGGYLT
                     INKPNNDKTRMLATTTDFKNKIIVPIEFKNTTGETKGFDTRDIFAYNGDETISFDSVI
                     SENLDNDGYSVVVKDGETAAASIVFGTNSKIKDIKVRYNSGLWK"
     gene            complement(2456..3235)
                     /locus_tag="VBC74_000374"
     CDS             complement(2456..3235)
                     /locus_tag="VBC74_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6941352.1"
                     /translation="MSSKKLENKEIFSKNLAFYMERKRVDRNTLCADLGLKYTTVRDW
                     LKGITYPRIGKIELLAEYFSINKSDLIEEHSTNGATSKVNFDPRQAILLSNYSKLNNV
                     RKNKLLAISETLLSEEQGKVINLQEKMAEYGSRKRVSLSVPGKVSAGTGYWQEDDYDT
                     EVDFYADEIPDEKNYDTVAVVVGHSMEPKIKNGDFLFIKLADQVDINKIGIFQVNGEN
                     YVKKLKNDYLQSLNPKYADITPAEGDDFRTIGEVVDIYREG"
     gene            3596..3745
                     /locus_tag="VBC74_000375"
     CDS             3596..3745
                     /locus_tag="VBC74_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941353.1"
                     /translation="MSEQNEKFIGVLKDMHMQILRDFVNAEDPLTRCELAKGFLEIGD
                     YLSKQ"
     gene            complement(3734..3946)
                     /locus_tag="VBC74_000376"
     CDS             complement(3734..3946)
                     /locus_tag="VBC74_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941354.1"
                     /translation="MKISNSKDLALAIVASSSPTLSIEDKIKLYEDSLEAIKQHNLPF
                     IEAEKQEQINNGKVIAEALERGESLF"
     gene            4005..4163
                     /locus_tag="VBC74_000377"
     CDS             4005..4163
                     /locus_tag="VBC74_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941355.1"
                     /translation="MNWKKLMFGDLEHTFTSRNGKEKTSIEFEGGLLPALLVLGGITW
                     LIAWFITK"
     gene            complement(4261..4902)
                     /locus_tag="VBC74_000378"
     CDS             complement(4261..4902)
                     /locus_tag="VBC74_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941356.1"
                     /translation="MNFFNFLLCVFKFTSEYLIKNWIALIALFLSYSNYRRNNLQVEL
                     IAAPVSDWILSVILDNGESIYNPNGTLRANIKIINPSNVDVSYFDLIVFDKNRKYQHY
                     YQKQNNIINDLTGREAIAAVQPDGNTILIEVPEADCGVLKAHSMTRMDLIIQTSEITD
                     RLFVAFKVAKKKKLFKANKAGYVNSPYQSFSASFPVELSKKPHYEDILKDLHE"
     gene            4960..5163
                     /locus_tag="VBC74_000379"
     CDS             4960..5163
                     /locus_tag="VBC74_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941357.1"
                     /translation="MEEILSSIAKSLESIATEFKAQNSYREEMKQNMEQMEKIILDIQ
                     SDPFGLKHLKDKALSEKASKQKG"
     gene            complement(5147..5506)
                     /locus_tag="VBC74_000380"
     CDS             complement(5147..5506)
                     /locus_tag="VBC74_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941358.1"
                     /translation="MDKLTNDAKFLLSSMFVKYNERRKDKISKEKSRNFEDIQFIKEN
                     IMNEWSEEDVLDTCFELRKHGYISATAASDTLYLISLTTEAIAELEKQDQAKTLSGRI
                     EYWLEFAKKIKDAIPFA"
     gene            5579..5785
                     /locus_tag="VBC74_000381"
     CDS             5579..5785
                     /locus_tag="VBC74_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6941359.1"
                     /translation="MTKMTLKALRATKNWSQEEAARALKVSKDTWGNWERGNTEPSVT
                     KAYQIAYIFDISLDDIIFLPNIAV"
     gene            5839..6798
                     /locus_tag="VBC74_000382"
     CDS             5839..6798
                     /locus_tag="VBC74_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941360.1"
                     /translation="MQEITTQNDFDYSLVDFETQEFLQERSNIIYGIQSKSAYEIGKQ
                     LVKAQEVLAKNRYGCFEEWYSSLGFRKTKAYEYINHYNFIRSQSEQLNIETFEELPKK
                     LQSEMSKPSANPELNQKVFDGDITTHKQYKELERQLKLAEADKERLKQQNERLAEQAL
                     SAKVVEKEVVIEKVPDDYESTKQLNRTLLDKNKELSTTLEEAEWELDSKKLELSTIKL
                     ESQRAIEVTNQIRHLEGKKEKLENLVTSISELSSIISDVQNFFDTKMAPLRFKPIINN
                     VNAHYSVTEVTKMVNTVQTWCDEMYKIIPSGNRKTVEEVIINE"
     gene            6791..7312
                     /locus_tag="VBC74_000383"
     CDS             6791..7312
                     /locus_tag="VBC74_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941361.1"
                     /translation="MSKELSEMNEKDALEVVKGMTIEDMMIEVLSSQKQVKASQEAIK
                     KDVEEVRSLAIEIDKKVHIDDVEASEIKSIISRQSYEFAKEYFDNRKVVPSANLFASK
                     KGQFIRVQHSRLKHHFNVTKYTHIKHTEAEKAISYLKSLSYGLFTPFETRETPKQKEI
                     IELENKNNGDDVA"
     gene            7331..7522
                     /locus_tag="VBC74_000384"
     CDS             7331..7522
                     /locus_tag="VBC74_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941362.1"
                     /translation="MNEKITDEQLNKYLEIRTELGEENFKFINACYDEVLHLKKEEAT
                     KNIKFEITDFSKFRDFAKK"
     gene            complement(7550..7726)
                     /locus_tag="VBC74_000385"
     CDS             complement(7550..7726)
                     /locus_tag="VBC74_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941363.1"
                     /translation="MISSQRIKETLDRHDVSESDKLSVALAEILNKHLSGKNLSETVH
                     EHDKRMARMRGEIM"
     gene            7841..8017
                     /locus_tag="VBC74_000386"
     CDS             7841..8017
                     /locus_tag="VBC74_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6941364.1"
                     /translation="MKKKLNKILIDKGMSKKELSEKTGISYNTIMNIGKRDISFNKMK
                     KIADVLDISLNEFR"
     gene            8096..8353
                     /locus_tag="VBC74_000387"
     CDS             8096..8353
                     /locus_tag="VBC74_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941365.1"
                     /translation="MQSQLTYDLLKKQIAEELFDEFKELIQEKDLANRWVNQTALVNE
                     YGYSWQTIKRMESYGLKSFKNGKDKMYCLADVNEIKHLMKQ"
     gene            8383..8526
                     /locus_tag="VBC74_000388"
     CDS             8383..8526
                     /locus_tag="VBC74_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941366.1"
                     /translation="MKLFNFIFAKPKKQEEKPKWTIETHGWEANARRYDNMIEHNNRG
                     RTC"
     gene            8602..8802
                     /locus_tag="VBC74_000389"
     CDS             8602..8802
                     /locus_tag="VBC74_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941367.1"
                     /translation="MNNDWKKRYINNQIDFFNDIKTCVKERWPNMKGNDVDKISIAIY
                     RNWPVSDINSTLEVIETDLEKL"
     gene            8799..9089
                     /locus_tag="VBC74_000390"
     CDS             8799..9089
                     /locus_tag="VBC74_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941368.1"
                     /translation="MKCYVNKQKKLAIDMNYKDKFGKFSSDSIQILEGKLTDSIQIDV
                     ENAMKEIIDKYSQLFDTPIIDDLFTEKEKQLKQSYDVETTLTEMFEVEYEDN"
     gene            9076..9759
                     /locus_tag="VBC74_000391"
     CDS             9076..9759
                     /locus_tag="VBC74_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6941369.1"
                     /translation="MKITKATEITKTHNWRILIYGKPGNGKTYLINYLKGKTLILDMD
                     HSSKTIAGNENIDIIQFDRTHPSDFMTEFLTELPELIKEYDNLVIDNITSWQSDWFIE
                     QGRKSKNGITNELQQYNMWTNYYLRVLTTIYSQPINIFVTAWESTQDLTLESGQMITQ
                     YVPDIRKQVLSQTLGLTDVVGRIQVNPKTGGHGILLEGSDGLYAKNRLDNRTVCKAEE
                     LFNFEGSDA"
     gene            9795..11198
                     /locus_tag="VBC74_000392"
     CDS             9795..11198
                     /locus_tag="VBC74_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6941370.1"
                     /translation="MNEARRHILKHNVMIVSPPGSGKSVVISDIAKSATQKNGYVLFL
                     VHRKELIDQITNSFKFHGIDMNKVDLMTVGKAKNRLDKLTKPTLIITDEGHHGKASTY
                     QMIYEYFSDVPRIGFTATPWRLSGDGFTDTYDVMVLGKTVEWLINNNKLAPYDYYSVL
                     SIDTAKLKVQNGDYSNKSIDESFGKKIFGDVVQEYIKKANGQKAILYAHSVEASQAFA
                     KEFQSMGINAIHADAKTPKAKRDKSMKDFRDGKIQVICNVDLISEGFDVPDCTVTILC
                     RPTKSLVLFLQQSMRSMRYQPNKKAIILDHVGNWNIHGLPDTPHHWENYFRGGWKKKS
                     NKTNTVHAKECPVCSALWPLSQQLCELCNHDFGLKEKQEKERIEAELELIKRERFRIK
                     QLANKKFGKDLKTNWEIAQARVKDAGKGKPLYKLIYFYLKTDWVETNVNELAEVTGKS
                     EKEIYSAYNWLKKKLRG"
     gene            11203..11685
                     /locus_tag="VBC74_000393"
     CDS             11203..11685
                     /locus_tag="VBC74_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HEO6941371.1"
                     /translation="MAGFTTDFSEVKEHAEFKEQPYEMIVYDAYEAVNDRNGKKRVVI
                     DYVVRNDIKQEMQNYHLWDEQYPNSQTGKYHIGILMGKAKALGIKEGQHYDSFEAFLN
                     DFKGRTAKVTVKLDEYNGNKYPKVRYANQSDVPESHHIWKEKTTGFTQAEIEEDDLPF
                     "
     gene            11703..13256
                     /locus_tag="VBC74_000394"
     CDS             11703..13256
                     /locus_tag="VBC74_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage resistance protein"
                     /protein_id="HEO6941372.1"
                     /translation="MTSNEFIEALSKLTTETDWGDPIFGESVLKAELRKHLFKIVPID
                     HNGYIHKLFYSEMVKDEDVMYFVSDGRKTYRFLFGDTILKTDKQGNEYLTYSVENNFP
                     PFAKLVIDYILGTYTFFENKLYDIRYKQFKLIDDFTLQTKYGFKDSGHILEILQGIHK
                     TLNIQPINYIEPYQIACKDFIIDLENSEIINQPPLQNVSYFKYYEVDYKTAINSKSIA
                     EEYLEYVIADSNSLNNAILQSYFIAQVACGVRPKTNFFISKSGVRTGKGLRHIALSGL
                     FNKIDVELDTLKSNGFEALQAWAMFSGGEMALATEQGDIQGNAMERVLKIIATEKTHV
                     ARAIGQNQSIVNLTSVLCIDTNRTVALSDEMNGRKVLIQFKDRPKTETDYERESVFRK
                     YWLAFTDRDKNPKIDGCIGFLLNSLERFQKIGKWYQWKDVEVFNDIDLDEFQVALINA
                     LQEVDFVQRTDNKEVIDLSLQVYGKSNHAVSKAISEIGVRSRSKKVNGKTVRGYEIEN
                     KTRFDKYIL"
     gene            13525..14394
                     /locus_tag="VBC74_000395"
     CDS             13525..14394
                     /locus_tag="VBC74_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEO6941373.1"
                     /translation="MYHTTALSFLKKGYQVIPLRKDTGTPMIKFKDIQITEEVIKNTN
                     WFNCDYALLMRGIWCIDIDTHDMDEKLAKELYIMIKKMGIDLLSVLSTDKYDNGLDGY
                     SSIIRHEYKNELISNFKNTFAELTASGGMHILFKKRDDINYTQKIGVMPGVDIKANDN
                     NFVKIFPSDGREVLQAVKTLPYYDGKFEEEVFKPKQEGITTYFGCSVTYTSNGSHEGR
                     EAYERVATGTSYNRNDDLFKGACWAFENGIDIDDLTSIIGTVKGRDVFTREEFELTIE
                     SAKRKVSYVTIRT"
     gene            14348..14695
                     /locus_tag="VBC74_000396"
     CDS             14348..14695
                     /locus_tag="VBC74_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEO6941374.1"
                     /translation="MNQRNEKSATSLSEHDIQNLIRMELSQAGYMVFRANVGKVKTAD
                     GRFFDTGLPKGFCDLFGFKPNGQIFFIEVKNETGRIRPEQKNFMEVMASKGALAGVAR
                     SVEDALKIVNGYH"
     gene            14774..15076
                     /locus_tag="VBC74_000397"
     CDS             14774..15076
                     /locus_tag="VBC74_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HEO6941375.1"
                     /translation="MADKINAESMQAAYNENYQMFLAKNADYGNSFEKSLNDFGFIAG
                     IVRIGDKYNRLYNLISSDKNVSESLSDTLNDMANYCVMLAVWLEEEERHRNLEHGG"
     gene            15211..15651
                     /locus_tag="VBC74_000398"
     CDS             15211..15651
                     /locus_tag="VBC74_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941376.1"
                     /translation="MIPNFRAFNKETKKMYGVDGFELSVRKIYRCSLADDEFRCGRLE
                     TFHFVEDNFDDYILMQSTGLKDKNGIEVFDGDIVKCCRLFNDSLSEYVGQVKFVDFGW
                     NIVDKADTHDPFYNYKDGCPDEIREIENIGNIHENPELLERLEG"
     gene            15673..15750
                     /locus_tag="VBC74_000399"
                     /pseudo
     CDS             15673..15750
                     /locus_tag="VBC74_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
     gene            15775..16260
                     /locus_tag="VBC74_000400"
     CDS             15775..16260
                     /locus_tag="VBC74_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6941377.1"
                     /translation="MSDIKILDACCGSRLFWFDKNEEHTTFMDIRQETFDIHGKHVNV
                     KPDVLGDFRNMPFENNIFNLVVFDPPHLKYVGQKSIMKAQYGQLDKDNWKEDISKGFE
                     ECMRVLKVGGTLVFKWSDCQINVKEVLSAIPFTPLFGQRRGTTHWMTFVKFAELTGNG
                     G"
     gene            16263..16586
                     /locus_tag="VBC74_000401"
     CDS             16263..16586
                     /locus_tag="VBC74_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941378.1"
                     /translation="MSLIDEVKECGSESHKKWFDRFFDDLKIEEKLKQSAMKGFSGYK
                     IAIPKDDEYLARRLDSQKTIDLLKQRLGDGFDVEITNLESSYKIFGKPVVLSRDLCIY
                     WTNGG"
     gene            16588..16752
                     /locus_tag="VBC74_000402"
     CDS             16588..16752
                     /locus_tag="VBC74_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941379.1"
                     /translation="MENYLCYWEKRGQNWYFLANCEDSPTGRIFNLTGVKFNYCPFCG
                     RRLKEVTNES"
     gene            16742..16906
                     /locus_tag="VBC74_000403"
     CDS             16742..16906
                     /locus_tag="VBC74_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941380.1"
                     /translation="MKVKDLHKVLAKVDPELEVWINEKDEGVLTKAKNVMTWNKHPDK
                     FFINAETYPE"
     gene            16921..17430
                     /locus_tag="VBC74_000404"
     CDS             16921..17430
                     /locus_tag="VBC74_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEO6941381.1"
                     /translation="MNIEEAKKIVDKLSVDSDELWKIPMIPAHKVKALLDTLDQPKPE
                     VPQMIFDVIKSFDDDVDYLHQHMSRQSDEVREWLTHNEREFYEAWLAYPNITIEKEKL
                     YTVEIPNPNERQLSFVLMRQLSGNVSIKVMHRDNLDLLKTDNDLQLTESEIRKDFDWA
                     WQFREEVAE"
     gene            17427..17693
                     /locus_tag="VBC74_000405"
     CDS             17427..17693
                     /locus_tag="VBC74_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941382.1"
                     /translation="MKEKTIFISKEYANDFNNDKYNLSSGYYFRNGKKHDIAIVKYGE
                     KDYLKNTDLAYVVCDKIVDADSIGFVYHGEYETWHFKLLNTEAN"
     gene            18083..18517
                     /locus_tag="VBC74_000406"
     CDS             18083..18517
                     /locus_tag="VBC74_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEO6941383.1"
                     /translation="MTFFPEINIQKTKSNAKRKLREYPRWRRIANDVDTQKVTATYSF
                     EPRQSHGTPSKPVERLALNRVSAEQELEAIEQSVSMILEPEKRRILYDKYLSPYKNAD
                     KVIYTELCMSESFYYDTLDAALLAFAELYREGSLIVEQGVFD"
     gene            18683..18761
                     /locus_tag="VBC74_000407"
     tRNA            18683..18761
                     /locus_tag="VBC74_000407"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            19141..19479
                     /locus_tag="VBC74_000408"
     CDS             19141..19479
                     /locus_tag="VBC74_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEO6941384.1"
                     /translation="MRPQKLTIAGGRRTTVDYDDRSAEYRDYNRNRWKYDKQVKQFYN
                     SKLWRETSKQVLLQNDYVCAMCGGEATMTDHIVSVKKDWNRRLDWNNLQASCKACNDS
                     KAIRERCKNN"
     gene            19650..20117
                     /locus_tag="VBC74_000409"
     CDS             19650..20117
                     /locus_tag="VBC74_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEO6941385.1"
                     /translation="MGRKLKVVETTKKHLTKEEKIARETAQNKASDGLDKLQITPPRH
                     LNEVARAEYRRIINDLQTLPVRNLDRGLLELYCSWYAIYKETTKKLDEIGYFTNDPDK
                     GLIPSPLILTLEKATANIRSSASQLGLTVDSRMKMFIPKEEEKQKSIFDKFGG"
     gene            20132..21886
                     /locus_tag="VBC74_000410"
     CDS             20132..21886
                     /locus_tag="VBC74_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEO6941386.1"
                     /translation="MTIEYDYSAISDIYKDDTFYYAKKIVDEEIKASKKVFKACLRHL
                     NDLKRIEDEDFKFIYLPQKASDPINFIEILPDVKTGKPYPLAMFQKFIIGNLYGWRKK
                     TDHSLRRFRKAMISVARKNGKTILIAGILLYEFLFGHNPSMSRQLFCTANDRTQAKIA
                     WDMAKKQLASLRAKDADVRKATKIVRDELKNLHDESYIRALSRDTGAVDGFEPYVGVL
                     DEFAASKTNEMLELLESGQGQLDNPFILIISTAGMDLNVPMHTIEYPYITKILDGEIT
                     DEGYFGYVAEQDNEEEIKDETNWIKSNPILEVDTLHDKLMDYLRTRRKVSLETGEVNK
                     VLIKNFNMWRQSSEESYIDKQSWELAKIDKPDTYKRRVWLGVDVGRVSDLFAISPVVM
                     MDDYWYVDSFSFVATKYGLTAKEKRDGVSYSNLERQGYCEITTLESGVIDDERVLEKI
                     EELIYINEWEVHGICFDPYQFGTLLTMIEKRHPEWPLIEVSQTTMVLNMPTKQFRDDL
                     KKGKIKHSGNPLLTMAANNAYIKTDNNGMRIDKNKNSNKIDPLDAVLDGYAVCYLEPF
                     DGSGYWTSEKILGGETLF"
     gene            21883..22050
                     /locus_tag="VBC74_000411"
     CDS             21883..22050
                     /locus_tag="VBC74_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941387.1"
                     /translation="MIGFILKNIHTLILLVGLGLLIYGLFLFGDKVGFIASGIILVIL
                     AIYVDSVGGRK"
     gene            22047..22277
                     /locus_tag="VBC74_000412"
     CDS             22047..22277
                     /locus_tag="VBC74_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941388.1"
                     /translation="MNKRIKKKRKLEHYIASLIAENVMFSKELIKQHERIEQLEKIVE
                     HNAQATNNELSRIKKHSKKKWKNNVTLPIERR"
     gene            22284..23504
                     /locus_tag="VBC74_000413"
     CDS             22284..23504
                     /locus_tag="VBC74_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO6941389.1"
                     /translation="MSFFQPLGTSKVSYDDYIASVLAGDVSQQYLGVSALKNSDVLTA
                     TSIIAGDIARFPLVKKDINGDIIHDEDINYLLNVKSTNNASARTWKFAMAVNAILTGN
                     SFSRILRDPKTGQALQFQFYRPSETTVEETENHEIIYTFTDTLTAKEIKCFAHDVIHW
                     KFFSHDTILGRSPLLSLGDEIDLQTGGINTLIKFFKDGFSGGILTMKGAQLSGEARQR
                     ARQDFEKMREGSVGGSPLVFDSTMEYTPLEIDTNVLQLITSNNFSTAQIAKALRVPSY
                     KLGVNSPNQSVAQLMEDYVTNDLPFYFDAITSEIGLKTLNDGDRRLYHVEFDTRSVTG
                     RNVDEIVKLVNNQILTPNQGLVELGKQKSKDPNMDRYQSSLNYVFLDKKEEYQDQVGV
                     KGKRGEVNAKENKP"
     gene            23482..24147
                     /locus_tag="VBC74_000414"
     CDS             23482..24147
                     /locus_tag="VBC74_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEO6941390.1"
                     /translation="MPKKINLKGPLIANNSQEVYDYYGMEAVSAKSIIDKLPEDNSDI
                     ILEVNSNGGLVTVGSEIYTALRNYKGKVTAEITGMAASAASVAVMGADKVVMSPTAQM
                     MVHKALFNWVSGNSDDLDKASSALKSSDKAIVNAYVAKTGKSEDEIMQLMRNETFMSA
                     QDAVESGFADEVMSFEAIASIDNQMLPQAVIDDYYASRSKRKQEINNMLLEIEKEEIL
                     QGL"
     gene            24171..25355
                     /locus_tag="VBC74_000415"
     CDS             24171..25355
                     /locus_tag="VBC74_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEO6941391.1"
                     /translation="MFDEKIEEIKADITELNKVIAAKTAEVKAALESDDLETARSVKA
                     EVEATKAKLVESENDLKLYESSVEVGGAENIGGKEVTQEEKTYRESVNDFIRSKGKIV
                     NDSLRFEGKDEVLMPINETTPVEPKKDGVKKTDVKPVSSEEILYTPAREVKTVVDLKQ
                     FTSIHQAKKASGKWPVLQRATEKMVSVEELEKNPKLGKPQFTNVAWEIKTYRGAIPLS
                     QESIDDADVDLVGIVAETIGQMKVNTTNDAIATVLKEFEAKTVKNLDEIKKLLNVELD
                     PAYNVSFIVSQSFYQTMDTLKDKNGRYLLQDSITSVSGKVFLGKPVFVLADEVLGKDT
                     AFVGDFKRGVLFADRKDLGLRWADNEIYGQYLQAVLRFGVSKVDDKAGYYVTFKPEQL
                     PS"
     gene            25369..25530
                     /locus_tag="VBC74_000416"
     CDS             25369..25530
                     /locus_tag="VBC74_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941392.1"
                     /translation="MAQPEVSSATVVGKPTKDSTIEDIKKYLTSQNIDFSGKTLKADL
                     LKLAGVEEV"
     gene            25533..25835
                     /locus_tag="VBC74_000417"
     CDS             25533..25835
                     /locus_tag="VBC74_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEO6941393.1"
                     /translation="MTVSKELLDSVKLYCKIDFDFEDDIIEEMIASAQEQICFAIEEG
                     STPEMFKNYAKFSLAVKKQVKEEYDHRGLSADTNRYPLANGVLNIIHQLRLRGDGA"
     gene            25832..26179
                     /locus_tag="VBC74_000418"
     CDS             25832..26179
                     /locus_tag="VBC74_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEO6941394.1"
                     /translation="MITRKMNVRIAIFSQTGGQNEDGEVVSAIRKDLYTCWAEVSKTQ
                     LRDFNYQSRYQNASNLPTNKDTKVFLIRYNPKLSIDNTMFIEHNKHIYKIDKIEFDES
                     GKEMIILSGVSIS"
     gene            26176..26553
                     /locus_tag="VBC74_000419"
     CDS             26176..26553
                     /locus_tag="VBC74_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEO6941395.1"
                     /translation="MTKGLDEILANITKLEVKSPKIAKEAVTQVAEEFEKALTKNTPV
                     DYSVHDTKLKYDTAISGFKGANVGITSKKIGYGRKTGWRAHYPNNGTIYQKGQDFKEK
                     TINEMTPRAKEIYAKKIKEGLGL"
     gene            26550..26975
                     /locus_tag="VBC74_000420"
     CDS             26550..26975
                     /locus_tag="VBC74_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941396.1"
                     /translation="MIAETTAYKLLKNDRTLNELLDKLRGGPFKNGFDQGIFTYDIPD
                     NPVDLRKVELAPFMRINTTYDGPSLYADDDMISNEQRITINFWCKTASQSDQIASCID
                     RILKNGGFERYTANEKPRYKDSDIDLLMNVRKYRYFDFY"
     gene            26991..27587
                     /locus_tag="VBC74_000421"
     CDS             26991..27587
                     /locus_tag="VBC74_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6941397.1"
                     /translation="MGKVKFGLRDFQYAVLGDNDKVNEKDKGIKALPGMKSAKLDITN
                     ELVTVMADDGPYVVLSGGITETKLEIEVLDLTSEARQDFFGIKADKGIERYNKSLTPN
                     NVACMFRTSDENGKAIWVGLLKGKFNIPGMDTKTKEGAPNPEPDKVTGNFVARGTDGD
                     VVVIGREGVNGFELKKFKELVFDGVTGLEKSSEDNSLM"
     gene            27640..27960
                     /locus_tag="VBC74_000422"
     CDS             27640..27960
                     /locus_tag="VBC74_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941398.1"
                     /translation="MYEITLKKGGVDKTFSKDFVNVEDNMLAVEHQVRQSAIFSDDKR
                     RLDDKEHRKLNESYLQMFVEMYGNQFNVDDLKQSNVSVLDKLTELFVDALGGEKEEDE
                     KKEQ"
     gene            28170..32111
                     /locus_tag="VBC74_000423"
     CDS             28170..32111
                     /locus_tag="VBC74_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEO6941399.1"
                     /translation="MGNIGDLVATATLDISPFMSNTRNLKTYMKTLDNSLKAVENSFN
                     GHGSRVKGLKAIYTETGGALKSYQELLKKQSQKYNELKENIGDVNKATASQKQALINA
                     KSAMMETAAKVSELQSRLKALGAETSVFTRFGNAAEQAGKKMKSFGDSVSGVGAAFTK
                     GVTAPIVAGAGYALKAAVDYEDAFAGVKKTVDEVKDSNGKVVYSYDMLSKGIRQMAKE
                     IPASTTEISHVAEAAGQLGIKTKDVLGFTRVMIDMGKSTNLSSEEAATALARFANITQ
                     LDPSKYSNLGSSIVELGNNFATTEKEIVEMGLRLAGTGKVVGLTDPQILGLATAMSSV
                     GIEAEAGGSAFSRVMQKINTQVLSGGEDLWKFAKIAGKSADEFAASWKKNPQEAIIDF
                     VKGLKRFKEEGKDVTAHLQDMDINSVREIDTLQRLAGAGDLLGDAFKSANKGFSENKA
                     LMNEANKRYETTASKLQMLKNQVNDVAIEFGGPLIDALRDGLEAGKPIIKGVADLAKA
                     FSSLDKEQQQQIIKWGLIAAATGPALSIFGKGVGVIGGTIQGLGKLSKTLGTLSGSLG
                     AAKTGAAALGVGAEGATTALTGMSGAAALLGNPVTWGVVLGGAAALAVGYFAQKAYEA
                     HQRTQEWGTKVSQVQANELQSFKDKVDQTNQSMEGFRGGAEQVNSVKTAFQGLVAEIE
                     KLENKDLSEKIKLAEQFGFSQGTMDQIKKSSRQTVENVKQMSDEVINIYQNASNEHRR
                     LTEEEKAVVLANQNELINVQLSKLNYSAKEKKAIVKAMNGDLNSLNSQQLTKALEVTE
                     KWIKAENKSYNKLKSGLKKVYDSIKGNDKEAVKTREEIHKKQQQLEADHYLKMEAYGK
                     RYAAIQKKLLKGTAKYLDPQLQQAMVNDVKKQMKELGLSYEELMTKTTKAASKAQEVN
                     TMWARTTKKSTEDQKLANSQWNGLVWNPKTGKLKTNAKEEVAKALEAEGGWDRLKFIA
                     KNANLETNARITMAEVLVETGKWDTLTPEEKELVVDGHQGIQAIVESKQHLEIWNSLP
                     EEVKRILGDNKDFLNKKGVATRTLENWNALKPDEKKLLAKNLTKKGKDEAQKTINSLV
                     GKEVKVTAANKTLSGVNSAQRTLNSVQDKHVTIWASIKKTASDLWSKLTGYAVGTDYH
                     PGGLAMVNDQKGSLYKELVTLPNGQSFIPDGRDVILPLPRGSKVMKASATRDYMYDLG
                     IPKYANGIGFDNTKIANITQRMSELPKNTVTSTTDDKLYWMIEEMIAVLKSTKDNSVI
                     EQALDIAEQAIERPVELYLQDGQWVAKVADRITNYQTQRNSRNNRMKGMLN"
     gene            32108..33631
                     /locus_tag="VBC74_000424"
     CDS             32108..33631
                     /locus_tag="VBC74_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEO6941400.1"
                     /translation="MKFIYDGVDMSKFFRISRVERSIGNERTLSLNETFQLGTAIRGI
                     KTGAKIIKVHIEPLEINGVLTEQLKHELAGVLNVDKPKKMTFGDEPDKYYLGLAQGEI
                     STEKVARWYQRAVITFYIPDGVAHSITYKKFSDYTQEGNKLIFNLQNDGNTNAYPIIK
                     IKHNSENGYIGIANETGAFALGSSEEEDGTIVHRNESLFDYSKAIAQALEGAPNVAKL
                     NYMPPTYDTELKRMRIDNILGSGKGGEYVVIGNRGTTPGYTEHIGTRTFIINPDSNGE
                     YTLNEHLWWQQIFIATAQDQKGFLKLCVTGIDDEGNDEFLYGIETYKRKNGFETEYNF
                     FALDDDGVGWRFYKQFKFQADRNYHNPFSMNRSRAVEIFREEDKFRIYFNGAHHHVTV
                     PSLKGKKSRKIHLAMGTCSDSSKYINYNLFEKVNFEKMGVSHYNNIVNKYQPGDEVVI
                     NFENDTVKTKDLDSIQDMVLGSQPISIPPGKTEFVMQLSKFSQYAPNVEILMEERWL"
     gene            33622..37443
                     /locus_tag="VBC74_000425"
     CDS             33622..37443
                     /locus_tag="VBC74_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6941401.1"
                     /translation="MVIITLVIHDSKLHPVLLLDNEKQGTLNYFDDTWTRQLTTGSSV
                     FEFSVYKKTLEGDNPLNHKYQVLNDQAFVSFVHKDKVQLFNIMQIEETETTVRCYCEN
                     LNLELLNEYCNAYKATKAMSFEEYLVQFDILNWGALTIGTNEVKDKKLTLEWTGQDTK
                     LARILSIANNFDAEIEFETQLHNNHTFKAFIVNVYKEYEEGVSYGVGRDRSDIVLRYQ
                     KNVTGITKKLDKRQIYNAIRPYGKKTVKGERVISNPVTRKVTKTVGSNRTYLGGDLKY
                     YGHTIKKANVQSIINYAVQYNILPSGIICQLYLESLWGDSAVGKRDNNWAGISGGAQT
                     RPSGVKVTTGMARPPSEGGTYMHYASVDDFLKDYTYLLAKQGLYNVVGKKNIADYTKG
                     LFRVGGAKDDYAAAGYQSYTNLMTNIRNGINKVTGNILNTIDKLWQTPVQPITAVNVA
                     RRATKTMQALNEATRLKGRRIGSGQCYALSGWYAKKLDGAWIDSSIGGIRGRIGGGMA
                     AALIGTDYNWGAYGWKLDRSPNAGNLQAGGIYNVKANFGAPFYTTQWGHTGIIKSVSK
                     TRVTVLEQNFVGRMYVVENSYDINSFASGLQTVCYPREIAQGMAVNGATTQQVSGGTQ
                     ISYEEVVQEAQTESYEEEQIIYIDNSIYKEWKDENGKVEYYLKNGFLYAPLSRDRYPS
                     VLTGNETRDNWIRKDMEIETDSQDVLMSTGLKDLKAHAYPAITYEVDGYVDLEIGDVV
                     RIQDDGYQPPLILTARVIEQVLSKDNPNSNKTKFSNFVEKESQLASDLISDMLRLYDE
                     SIPYEIKLATSNGVAFKNGTGESVLTPSLQKNGKDYEAVYFYKNGDSLIDIGPSLIVK
                     ASDFNHVLNVTVEAYLNEELVASTQISFTDTEDGADGNTPYWHVAYANSADGTVDFSV
                     SDSANKRYIGQYTDYDAIGSNDPKKYKWTDMVGTVVVGTNNLIDGTKSFSGEDWFTSA
                     TLEDENISNYPFTFKKWISGQKVSHAKDIMVEQGVTYTFSAYVKREVAGNLYFYLYDI
                     ADGFITSDTPRETIIKNVDSSLRRFEITFTPTKTGRIRPRFAMVSSEQGSFSSGGFML
                     VRGNKTGDWQESEADKASNLDAKADGAFTVEQLNALAERARIAEAELQSKATLDTVND
                     WVKALQDEIKAREEGQKLSEQKLIDSSNRMIAVQQTIGEMQVRTDFVNKFMSQSEDGL
                     VIGQKDGTSSVRVDNDRISFYSSGKEVAYIAQSVLVIDSGIFTTKLQIGRYRIEQYEL
                     NPDINVVRYVG"
     gene            37456..39459
                     /locus_tag="VBC74_000426"
     CDS             37456..39459
                     /locus_tag="VBC74_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941402.1"
                     /translation="MTTYYSNSDRSYRLTYIVDEVSTSIADNSSQVRFRLYLTSRTNS
                     YAQYNFSGYAWVGGRYDFSAPSALGFNSNYLLIDKTIKVPHDADGSKTVVVAAKLSGP
                     GGYAPGTLNIPDQKFTLTKIPRTSTVAVSSGYFGDTLNVNINKTNDSFTYDVRYNVNG
                     ITGTVASDISGSTTFKTSLDWASTIPNTTSTPATIYVDTKSNGSVIGTSTGIFYLTVP
                     DNIKPKISSLTLSDTNQKASTIVGANNFVQIISNPVVTFNGASGAYGSTIQNFNAEII
                     GKNQSTQSNGGSLGILNFSGKATVKATVTDSRGRVSDPVTTEINVIPYSPPAFSFTVT
                     RAGAKNDQLVVTRNAKISPLIVDGVQKNKMTLTFKTAPLNTTSFTIDTSNASGTYTTV
                     AELINSTATLSGSYGANKSFDVYGLLSDVFSASGGGTPVKQTVSTESFPLSWHKNSVG
                     IGTLPKIDDTGSLNVAGNIYSDGKQIQQKQLALNNGGAFRHDTTDLNSLQDTGFYCVF
                     KGDNRPSGAGPGYLTVVRHETANYAYQHFYDRTNKTIFTRVLENGAWSGWSEYAKKDS
                     LPQSAPAVEDTGWQYIGNGFNYRKIGSMVTIKYDFATNGINQFTVGSMPTNLIPNEMM
                     FAVTAWTVQLNVLNVQVSADGRILWFNPSKWAVNVKGQINWII"
     gene            39471..39863
                     /locus_tag="VBC74_000427"
     CDS             39471..39863
                     /locus_tag="VBC74_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEO6941403.1"
                     /translation="MLEFLNRYPVLLEDKSVKETKAILAFTSSTIKANFEVTLPAEEN
                     DKKFAETLKTCEKLIFEQLYKDKAEAEQFEKINDAIAKSKAQSDKAENMIKLMSATVN
                     DLIKTMADGGKLNDTTLNNASENSSTHI"
     gene            40023..40325
                     /locus_tag="VBC74_000428"
     CDS             40023..40325
                     /locus_tag="VBC74_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941404.1"
                     /translation="MNIDILQIGAASGAILSVVGLWAFVVNPFKTAMQKNEDTMSALK
                     DTIKELAYELKDSQRDREKIHKILDIHEQRLGKTEDDIIVNKEQIKTLFNRRNKYD"
     gene            40318..40545
                     /locus_tag="VBC74_000429"
     CDS             40318..40545
                     /locus_tag="VBC74_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO6941405.1"
                     /translation="MINLKLRLQNKVTLMAILGAIFLLAQQVGIKLPSNIADIANTAV
                     TLLVLLGVVTDPTTKGLSDSEQALNYHEPKK"
     gene            40671..42005
                     /locus_tag="VBC74_000430"
     CDS             40671..42005
                     /locus_tag="VBC74_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysin"
                     /protein_id="HEO6941406.1"
                     /translation="MATYQEYKNRSNGNAYDIDGSFSAQCWDGYADYCRFLGVPYANC
                     TNTGYARDIWEQRHENGILNYFDEVETMQAGDVAIFMVVDGVTPYSHVAIFDSDAGGG
                     YGWFFGQNQGGTNGAYNLVKIPYSATYPTAFRPKVFKNAVTVTDNTGLNKGDYFIDVS
                     AYQQADLTATCQQAGTTKTIIKVSESIAWLSDRHQQQANTSDPIGYYHFGRFGGDSAL
                     AQREADLFLSNLPGKKVSYLVIDYEDSASADKQANTNAVIAFMDKIASAGYKPVYYSY
                     KPFTLNNVDYQQIIAKYPNSIWVAGYPDYEVRSEPLWDYFPSMDGVRWWQFTSVGIAG
                     GLDKNVVLLADDNSQVTVPKADKPQEAVSFNQRLDVNTKLDNSNTPYYEATLSTDYYV
                     ESKPNASSSDKEFIKAGTRVRVYEKVNGWSRINASQSDQWVEDKYLANATQV"
     gene            42169..42375
                     /locus_tag="VBC74_000431"
     CDS             42169..42375
                     /locus_tag="VBC74_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO6941407.1"
                     /translation="MSLVEEKLKAKVEQASGSLKEGAGKLTGDKELEAKGFVEKTIAK
                     GKELADDAKDAVEEAVDAVKEKLK"
BASE COUNT        14919 a         6961 c         8703 g        11822 t
ORIGIN      
        1 agacaccaag attgtcaaaa gcgacaattt aataaaatgc cttatgaaga aatcagtgaa
       61 gttagtcatt atgttaagag taaaggacta gttttagatg actatattgc ttatcgtgat
      121 actttaacaa aagtggaaga aactttgtca gatattgata aagaaaaatt tgaaaaactg
      181 attaatggtg aacgttttgc ggggaagaaa aaattcatac gaaatattca accttttttc
      241 aatgctttta aagcggatta atcgatttta tttctgcagt agatagctgc agaatttttt
      301 tatagggaaa gttcgctgat atattcctgt gaaagccttt gttttttgtt ataatataag
      361 attacctctc agaatcattg atatcaaggc ttttttaaag tctaaaaaca gacttactac
      421 gttccccact acgtttgtgt taaatagcga cgagttcggc caatttgtca gcaacattat
      481 cctttgattt tttggataag tgagtatata aatccatcgt gattgctatt ttagcgtggc
      541 ctaaccgctc ttgtgcaatc ttagcgggaa tcccagcctc aaacaacaac gaagcgtggg
      601 tatgtctaaa cccgtgggga gtaatccttt ttaatccatg cttcaatata aatctgtcca
      661 gtctttttct cacggtgtcg ctagcaaaat taaacaattt aaacgatccg tgtaaaggtt
      721 tgatactttg cttaatatat aatttggcta acgtcatggt ctcagcgtcc attgatatgg
      781 tgcgattgct ttttttggtt ttaggagatt gtacagccca tccttctttt gtataagcaa
      841 tggtttttgt aatgctgata gtattgttgc ggaaatcaaa gtcggtatct tttaaagcta
      901 ttaattcacc aatcctaaaa ccgccataac tcagcaatct ataaatcaga cgcatctcta
      961 gagtttcttc agcttccaca atcctcaaga aagcattcaa ttcctctttt gtgtaatatt
     1021 ttttggtgag tgtatccatg tttttgtaag tgcactttga tctcaacgtc ttgctcatgg
     1081 ggttattatc aataatacct aagttgacag caaatttaaa aattctgttt gctaaactta
     1141 gataaagcct ataaccgcta tatcgagata ggcaattcac aaatttttga caaatcatga
     1201 cagttatctt cgtcatttgc agttgtccta aatgtggctt taaaattgtt ttatagtagt
     1261 tttggttaac cagaaatgtt gacggtttga ctgtatttcg gtactgctca aaccacagtt
     1321 tggctacatc gtcaaatgta gttttatcgt tgcttttcca agcgccttga cgctgaaaat
     1381 catcaataag ttttacctcg gctcttttag cttctctttc tgttttaaac ccttgcctag
     1441 ttgtcctgac ttgtttacca gtcatgggat caacaccaag gtatgctctt aagcgatagg
     1501 cagttgtgcc atctttttta gtgtattttt caatcattgt tttttcctct ctctttgcgc
     1561 tggggagtgc tgattttgag ataggatatt ggcatcacct cctaaaaatg atataattag
     1621 gtacaagaaa acgacctttt taatggttgt ttcttatact agattcgccc cacgctcaga
     1681 ccctcccaaa tttgagcgtg gggctttttt tatttccata atcctgagtt atatctcact
     1741 ttgatatctt taatcttgct gtttgtccca aaaacaatac tagccgctgc tgtttctcca
     1801 tcttttacaa caacgctata tccgtcatta tctaaatttt cgctgataac tgaatcaaaa
     1861 gaaatagttt catctccatt gtaggcgaaa atatctcttg tatcaaaacc ttttgtttcc
     1921 ccagttgtat ttttgaattc tatcggcaca atgattttat ttttgaaatc tgttgttgtt
     1981 gctaacattc ttgttttgtc attatttggt ttatttatag ttaagtaacc tccacccaat
     2041 ttaacttcat caccaaaaga cgatacaact tgatctttat taagataaaa tttaatagct
     2101 ctgacagaca attcttcgtc aaaacgactg gtaaaatcgg atatggtttt cctaatacct
     2161 gacgtcgttt tattttcttg agttttttct aattttgcta ttttcttctc gttaacaata
     2221 ttagtctctg ccaatctgta aatgacaaaa aacgaaaatc ctaaaactaa tagtagtata
     2281 gcgtgtgtcc aaaaatacca ctttttataa aaaggttttt tcatatcata aatctcctaa
     2341 attaaatttt taaactcatt tactaccatc gcttcatcgc agatggtgtt taacctatac
     2401 gctttcataa atctataaac attaaaatct tctatgtcgt caagttttcc acagactacc
     2461 cttctctata aatatctacc acctcaccaa tggttctgaa atcatctcct tcagctggtg
     2521 tgatgtcagc atactttgga ttaagtgact ggaggtagtc gttctttaat ttcttgacat
     2581 agttttcacc gttaacttgg aaaataccaa ttttgttaat gtcaacttga tcagccagct
     2641 taataaataa aaagtcgcca ttttttattt ttggttccat tgagtgaccg acaacaaccg
     2701 caacggtgtc atagtttttt tcatctggta tttcatcagc gtagaagtca acctctgtgt
     2761 catagtcatc ctcttgccaa tatccagtac cagcagacac tttaccgggt acagatagac
     2821 ttacacgttt tctggagccg tattctgcca tcttctcttg caagtttatc actttgcctt
     2881 gttcctcaga taaaagtgtc tcagatatag ccagcaactt attctttcgt acgttgttaa
     2941 gttttgaata gttagataag agaattgctt gccttggatc aaagttgact ttggaagttg
     3001 ctccattagt agagtgttct tcaatcaaat ctgacttgtt aattgaaaag tattcagcca
     3061 aaagttctat tttccctatt cgtggataag ttataccctt gagccaatct ctaaccgtag
     3121 tatacttcag tcctaagtct gcacatagtg tatttctgtc tactcttttt cgctccatat
     3181 aaaatgcaag gtttttagaa aatatctctt tgttctctaa tttttttgaa gacatttttg
     3241 cacctcctta taatgtatat attacggcaa aaacgcaaaa aagtaaataa aaaaataaaa
     3301 aaaccgtaaa aaaacgcaaa aaaatacttg acattgcggt ttaaccgcag tataatataa
     3361 tcaagcttaa ggaattaagc aaaacgaaag gaggtacagc tgatgaagtc gaggctaaac
     3421 aaaaagccta aacacaaaga actagaagtc gaaatcaaaa ttctttggtt taagcttaga
     3481 ataaaacatc tgattactag gtaatcggat agggggtgaa actcccccac ccctaatagg
     3541 ggtaagttta gtttagcaca ttggctgtat ctccgcaaga atgaaaggag aagttatgag
     3601 tgagcaaaat gaaaagttta tcggagtgct aaaagatatg cacatgcaaa ttttgcgaga
     3661 tttcgtaaac gcagaagacc ccttaacaag gtgcgaactt gctaaagggt tcctcgaaat
     3721 tggcgactat ttatcaaaac aatgactcgc cacgctcaag agcttcggct ataactttac
     3781 cattattgat ttgttcttgc ttttcggctt caatgaaagg taaattatgt tgcttaatag
     3841 cttctaaaga gtcttcgtaa agtttgattt tatcttcgat agacaaagta gggctagaag
     3901 aagcaacaat agcgagagct aaatcttttg aattagaaat tttcattagc ttatcctcct
     3961 ttctgctagg ataagttgat tataacattt ttaggaggta caaaatgaat tggaaaaaac
     4021 taatgtttgg cgatctagaa cacacgttta ctagtcgtaa tggcaaagaa aaaacaagta
     4081 ttgaatttga aggtggctta ttgccagctc tgttagtgct aggtggtatc acttggctga
     4141 tcgcttggtt tattacaaaa taaaaactcc caaaaggagt ggaaaggagg gaaggcatgg
     4201 aaaaaattaa atacggtgta ctaggttctt ccaagatatt tcacacaaga aaatctgctc
     4261 tcactcatgc aaatctttta ggatatcctc gtagtgcggt ttttttgata attccacagg
     4321 gaatgacgca gaaaatgatt gataaggtga attaacatat cctgctttat tagctttaaa
     4381 tagttttttc tttttagcta ctttaaaagc aacaaagagt ctatctgtga tttcagatgt
     4441 ttgtatgatt aaatccatcc ttgtcatact gtgggctttt aatactccac aatctgcctc
     4501 tggaacctcg ataaggattg tattgccatc aggctgtact gcggctatag cctctctacc
     4561 tgttaaatcg ttaattatat tattttgctt ttggtaataa tgctgatatt ttctgttttt
     4621 atcaaaaaca atcaagtcga agtagcttac atcaacatta gaaggattga tgattttaat
     4681 gttagctctt aatgtaccat ttggattata tatgctttca ccgttgtcta aaataacgct
     4741 caaaatccaa tctgaaacag gagcagcaat taactcgact tgtaagttat ttcttcggta
     4801 gtttgaataa gatagaaaca gagctattaa agctatccaa ttttttatta gatattcact
     4861 tgtaaactta aaaacacaca ataaaaaatt aaaaaaattc atttcaacct cacattttta
     4921 tttaaattat accacagaaa cagaaagggg gtggggaaga tggaagaaat tttgtcaagc
     4981 atagcaaaaa gccttgaatc cattgcgact gaattcaaag ctcaaaattc ttacagagaa
     5041 gaaatgaaac aaaatatgga acaaatggaa aaaataatat tagatatcca atcagatcca
     5101 tttggactca aacacttaaa agataaagcg ttgtccgaaa aagcttctaa gcaaaaggga
     5161 tagcatcctt tattttctta gcaaactcaa gccaatattc tatcctgcca gataaagtct
     5221 tggcttggtc ttgtttttca agttcagcga tagcttcggt tgttaacgaa attagataaa
     5281 gcgtatcgct tgcagccgtc gctgaaatat agccatgttt tctaagttca aaacaagtat
     5341 ctagtacatc ttcttcagac cattcattca taatgttttc tttgatgaat tgaatatctt
     5401 cgaaattgcg agacttttct ttagaaattt tatctttacg tctctcgttg tatttgacaa
     5461 acattgaact taaaagaaac ttggcatcat ttgttaattt atccatatat tcacctcctt
     5521 tctgctcaca ttatagcaga ttagaggtac taaaaacaga tagaaagggg gtgggagaat
     5581 gacgaaaatg acgttgaaag ctttaagagc aacaaaaaac tggagtcaag aagaggctgc
     5641 aagagctttg aaagtttcca aagatacatg gggcaactgg gaaagaggta atacagagcc
     5701 aagtgttaca aaggcttatc aaattgccta tatattcgat atatcattag atgatattat
     5761 ttttttacca aacattgcgg tttaaacgca aactcttaga aatagaacaa aactaacaca
     5821 acactagaaa ggagaaaaat gcaggaaata acaacacaaa atgattttga ctattcactt
     5881 gttgattttg aaactcaaga atttttacaa gagcgttcaa atattatcta tggaattcaa
     5941 agcaaaagcg cttatgaaat aggtaagcaa cttgttaaag ctcaagaagt tttggcaaaa
     6001 aatagatatg gttgttttga agagtggtat tcaagccttg gatttagaaa aacaaaagct
     6061 tatgagtaca ttaatcatta taatttcatt cgttcgcaaa gcgaacaatt gaatatcgaa
     6121 acatttgaag aacttccaaa aaagcttcaa tcagaaatgt caaagccgtc tgccaatcca
     6181 gaactcaatc aaaaagtctt tgatggggat attacaactc ataagcaata caaagagtta
     6241 gaacgccaac tcaaactagc agaagctgac aaagaacggt taaaacaaca aaatgagaga
     6301 ctagccgaac aagccttgag tgctaaagtc gttgaaaaag aagtcgtaat cgaaaaagtt
     6361 ccagatgatt atgaatctac aaagcaactt aaccggactt tactagataa aaataaagag
     6421 ctttccacaa cacttgaaga agctgaatgg gagctagata gtaaaaagct tgagttatcg
     6481 acaataaagc ttgagtcaca acgagctatt gaagtaacaa accagattcg tcacctcgag
     6541 ggcaaaaaag aaaaacttga gaaccttgtt acttcgataa gtgagctgtc ctcaatcatt
     6601 agtgacgtgc aaaacttctt cgatacgaaa atggcgccac ttcgttttaa gccaattatc
     6661 aacaatgtca atgcacatta ttcagttacg gaagtaacca agatggttaa cacagtccaa
     6721 acttggtgcg atgaaatgta caaaattatt ccatctggaa atagaaaaac agtagaagag
     6781 gtaataatca atgagtaaag aattatcaga aatgaatgaa aaagacgctc ttgaagttgt
     6841 caaaggaatg acgattgaag atatgatgat tgaagtctta tcttctcaaa aacaagtaaa
     6901 agcttctcaa gaagccatta agaaagatgt tgaagaggtt cgttctttag caattgaaat
     6961 tgataaaaaa gtccatattg atgatgtgga agctagtgaa attaaaagca ttatcagtcg
     7021 tcaatcctat gaattcgcca aagaatattt tgataataga aaagttgttc caagcgcaaa
     7081 tctattcgct agcaagaaag gacagtttat tcgtgtacag cattcacgat taaaacatca
     7141 tttcaatgtg acgaagtaca ctcatatcaa acatacagaa gcagaaaaag ctatcagtta
     7201 tttaaaatca ctatcttatg gtttattcac accatttgaa actcgtgaaa caccaaaaca
     7261 aaaagagatt atcgaactgg aaaataaaaa taacggtgat gatgtcgctt agaactagaa
     7321 aggaaacaac atgaacgaaa aaattacaga tgaacaactt aacaaatact tagaaatcag
     7381 aactgaactt ggggaagaaa actttaaatt tattaacgct tgctatgatg aagttttgca
     7441 cctaaaaaaa gaggaagcga caaaaaatat taagtttgaa attacagatt tttcaaaatt
     7501 tcgtgatttt gctaaaaaat agccctcaga attaactgag agcttctagt tacatgattt
     7561 ctccacgcat acgtgccata cgtttgtcgt gttcatgcac agtttcagag agatttttgc
     7621 cagataaatg tttattgagt atctctgcca aagctactga taatttatct gattcagaca
     7681 cgtcgtgtct atccaacgtc tctttaattc tttgtgaact aatcatacct tatcctcctt
     7741 cctataggat agctacatta tataacagaa aggtacaaaa cacaatatgt tgtgtatctt
     7801 agacgattaa aaacacaata tgttgatttt taaaagtatt atgaagaaaa aattgaacaa
     7861 aattctcatt gataaaggaa tgagtaaaaa agagttatcg gagaaaacag ggattagcta
     7921 caacaccatt atgaatatcg gtaaaagaga tatttcgttt aacaaaatga aaaaaatcgc
     7981 cgatgtctta gacattagcc taaacgaatt cagataaaac aaaaaaagcc actgcgggaa
     8041 cagtgactaa ccaaaataat ttaactaatt ataccacgaa agaggtgata aatccatgca
     8101 atcgcaactt acttacgatc tactcaaaaa gcagatagca gaagaacttt ttgatgagtt
     8161 taaggagctc atacaagaaa aagatttagc gaatcggtgg gtcaaccaaa cagcactcgt
     8221 caatgaatac ggctactcat ggcaaactat aaaacgcatg gagagctatg gccttaaatc
     8281 ttttaaaaac ggtaaagaca aaatgtactg tcttgctgat gttaacgaaa ttaaacactt
     8341 aatgaaacaa taagcgctgg ggagtgctag aggagaaaca acatgaaact atttaatttt
     8401 atttttgcaa aaccaaaaaa acaggaagaa aaaccaaaat ggacgattga aacacatggc
     8461 tgggaagcta atgcacgacg ttacgacaat atgattgaac ataataatag agggcgtaca
     8521 tgttaaaaga agtattacta gaaattgaat tcttacgtga tgaaaataga cgactaacta
     8581 atgagttgct ggaggtatta aatgaataac gattggaaaa aaagatacat taacaatcaa
     8641 atagactttt tcaatgacat caagacatgt gtcaaagaaa gatggccaaa tatgaagggg
     8701 aatgatgtcg ataaaatttc gatagcgatt tacagaaatt ggcccgtaag tgacatcaat
     8761 agcacactag aagtgataga aacggatttg gaaaaattat gaaatgttat gtcaataaac
     8821 aaaaaaaact agcaatagac atgaattata aagataaatt cggaaaattt agttctgact
     8881 ctattcagat tttagaaggt aaattaactg attctattca aattgatgtc gaaaatgcta
     8941 tgaaagaaat tatcgataag tatagtcaat tgtttgatac gcctataatc gatgatttat
     9001 tcacagaaaa agaaaaacaa ttaaagcaat cttatgatgt cgaaactaca ttgacagaaa
     9061 tgttcgaggt ggaatatgaa gataactaaa gcaacagaga ttacaaagac tcataattgg
     9121 cgcatattaa tctatggcaa acccggaaac gggaaaactt acttaattaa ttatttaaaa
     9181 ggaaagacac tgattttaga catggaccat tcatctaaaa cgattgccgg aaacgaaaac
     9241 attgatatta tccaatttga caggacgcac cctagcgact ttatgactga attcttgaca
     9301 gaattaccag aacttatcaa agaatatgac aatttagtca ttgataatat cacaagttgg
     9361 caatcagact ggtttatcga acaaggtcgc aagtctaaaa atggaatcac aaacgaattg
     9421 caacaataca acatgtggac caattactat ttacgagtat tgactacaat ttacagccaa
     9481 cctattaata tttttgtgac tgcttgggaa tcaacgcaag atttaacgct cgagagcgga
     9541 caaatgatta cacaatacgt cccagatatc cgcaaacaag tcctaagcca aacgctaggt
     9601 ttaaccgatg ttgtcggacg tattcaagta aatccaaaaa ctggtggaca cgggatcctt
     9661 ttggaaggaa gcgatggact gtacgcaaaa aataggcttg ataataggac cgtttgtaag
     9721 gcggaagagt tgtttaattt cgaagggagt gatgcgtaac ggtataccat ttacacgaat
     9781 atcaaacaga acttataaat gaggcaagaa gacatatttt aaagcataat gtgatgattg
     9841 ttagtccacc tggaagtggt aagtcagtag tcatttctga tattgctaag tcagcgactc
     9901 aaaaaaacgg atacgtttta ttcttggtcc atcgcaaaga attaattgac caaatcacta
     9961 atagttttaa attccatgga attgacatga ataaagtgga tttaatgaca gttggtaaag
    10021 ctaaaaaccg cttggataaa ttaacaaaac caaccttaat tatcacagat gagggtcatc
    10081 atgggaaagc tagtacctac caaatgatct atgaatactt ttcagacgtg ccgaggattg
    10141 gttttactgc tacaccttgg cgtctatcag gcgacggttt tacagacact tacgatgtta
    10201 tggttcttgg taaaacggtc gagtggctta tcaataataa taaacttgca ccatatgatt
    10261 attatagtgt tctatcaatt gatactgcga aattaaaagt acaaaacgga gattactcca
    10321 ataaatcgat tgacgaatca tttggtaaaa agatttttgg tgatgtagtt caagagtata
    10381 taaaaaaagc gaacggtcag aaagctattt tatacgctca ctcggtagaa gcatcgcagg
    10441 catttgctaa agaattccaa tctatgggaa ttaatgcaat acacgcagat gcaaaaacgc
    10501 ctaaagccaa acgggataaa agcatgaaag atttccgtga cggtaaaata caagttatct
    10561 gtaacgttga tttgatatca gaaggtttcg atgtcccaga ttgtacagta actattcttt
    10621 gtagacctac aaaatcatta gtattatttt tacaacagtc tatgcggtcg atgagatatc
    10681 aacctaataa aaaagctatc attttagatc acgtaggaaa ctggaatatt cacggcttac
    10741 ctgacacacc gcatcattgg gagaattatt tccgaggagg gtggaagaag aagtcgaata
    10801 aaactaacac ggtacacgca aaagaatgtc ctgtgtgttc agctttgtgg ccacttagtc
    10861 aacagctctg tgaattatgc aatcatgatt ttggattgaa agaaaaacaa gagaaagaac
    10921 gcatagaggc agaacttgaa ctcataaaac gtgagcggtt tagaatcaaa caacttgcta
    10981 ataagaagtt tggtaaagat ttaaaaacaa actgggaaat tgcccaagct agagttaaag
    11041 acgctggcaa aggaaaacca ttatataaac ttatctattt ctacttaaaa actgattggg
    11101 tagaaacaaa cgttaatgaa cttgccgaag taacaggcaa gtcagaaaaa gaaatatata
    11161 gcgcctacaa ttggctaaaa aagaaattaa gaggataaaa acatggcagg atttacaaca
    11221 gatttttcag aagttaaaga acacgcagaa ttcaaagaac aaccatacga aatgattgtt
    11281 tatgatgcat atgaagcagt aaatgaccgt aacggcaaaa aacgtgttgt tattgactat
    11341 gttgttcgaa acgatatcaa gcaagaaatg caaaactacc acttatggga tgagcaatat
    11401 cccaactcac aaactgggaa gtatcatatc ggcattttaa tgggtaaagc aaaagcactt
    11461 ggtatcaaag aggggcaaca ctacgatagt tttgaagcgt ttttaaacga cttcaaagga
    11521 cgcactgcaa aagtaaccgt taaacttgac gaatataacg gaaataaata cccgaaagtt
    11581 cgttacgcaa atcaaagcga tgtgcctgaa agccaccaca tatggaaaga aaaaacaact
    11641 ggatttacac aagctgagat tgaagaagac gatctaccgt tctaatttag actgggggat
    11701 aaatgacatc aaatgaattt attgaagctt tatcaaaatt gaccaccgaa accgattggg
    11761 gagacccaat cttcggcgag tcagtgctta aagctgaatt acgaaaacac ttatttaaaa
    11821 ttgtcccaat tgatcacaat gggtatatcc acaaactgtt ttattcagaa atggttaaag
    11881 atgaagatgt catgtatttt gtgtcagatg gacgaaaaac ttatcgcttt ttatttggag
    11941 atacaattct aaagactgat aaacaaggca atgaatatct cacatactcc gttgaaaata
    12001 attttccgcc atttgcaaaa ctagttatcg actacattct aggtacttac acgttttttg
    12061 agaataaact ttatgacatt cgatataagc aatttaaatt gattgatgat tttacacttc
    12121 agactaagta tggtttcaaa gactctggtc acattttaga gatactacaa ggtatccaca
    12181 aaacattaaa catccaacca atcaattata ttgaaccata tcagattgct tgcaaggatt
    12241 tcataatcga ccttgaaaat tctgaaatca ttaaccaacc gcctttgcaa aacgtatctt
    12301 attttaagta ttacgaagta gactacaaaa cagcaataaa cagtaagtct attgcagaag
    12361 aatatcttga atacgttatt gcagatagca attcgttaaa caatgcaata ctacaatctt
    12421 attttatcgc ccaagtggcg tgtggtgtta gacctaaaac caacttcttc atctcaaaat
    12481 ctggagtaag gactggtaaa gggttaaggc atatagcttt atctggtcta ttcaataaga
    12541 ttgatgttga gctagataca ttaaaaagta atggatttga agcgttacag gcgtgggcga
    12601 tgttctcggg tggagaaatg gctctagcga cggaacaggg agatattcaa ggcaatgcaa
    12661 tggagcgtgt acttaaaatt attgcaacag agaaaacaca cgttgcacga gcaattgggc
    12721 aaaatcagtc aattgttaat ttaacgagtg ttctgtgtat tgacacaaac cgtactgttg
    12781 cgctgtcgga tgaaatgaat ggacgaaagg ttttaattca gtttaaagat agaccaaaaa
    12841 ctgaaactga ttatgaaaga gagagcgtat tcaggaaata ttggctagca tttactgatc
    12901 gtgataaaaa tccaaaaatc gatggttgta tcggttttct gttaaactca ctcgagcgtt
    12961 tccaaaaaat tggtaaatgg tatcaatgga aagatgttga agtatttaat gatatcgatt
    13021 tagacgaatt tcaagttgct ttaataaatg cattacaaga agtcgatttt gtacagcgaa
    13081 ctgataacaa agaagttatt gacttatcat tacaagttta cggaaaaagc aatcatgcag
    13141 taagtaaagc catatctgaa attggtgtac gtagcaggtc gaagaaagtt aacgggaaaa
    13201 cagtcagagg gtatgaaatc gaaaataaaa cacgttttga taaatatatc ctttaaaaag
    13261 gtaacgcatg gtcacgcaga gttacgcaaa attttccatg ttgcgtaacc cactcaatcc
    13321 ctttagtacc aaccgatttc agcctttatt caagaaaggt tacgcagtaa caaacaataa
    13381 gtctaattaa tgatttatta attatttatt tatatatagg ggtagggtag ggtgattttg
    13441 cgttactgcg taaccatcct tctctaatgc tctatgtatc aacgttttag aggttacgta
    13501 gtaaaaaagg aggtgcgtta ccttatgtac cacacaacag cactttcatt cttaaaaaaa
    13561 ggatatcagg ttataccgct tagaaaagat actggaacgc caatgattaa gtttaaagat
    13621 atccaaataa cggaagaagt gatcaaaaat acaaactggt ttaattgtga ttatgcttta
    13681 ttaatgcgtg gcatttggtg tattgatatt gatactcatg acatggacga gaagttagct
    13741 aaagagttat acataatgat aaaaaagatg gggattgatt tattatctgt attatcgact
    13801 gataagtatg acaatggact agatggttat tcgtcaatta ttaggcatga gtataaaaac
    13861 gaattaatca gtaattttaa aaatacattc gcagaattaa ctgcaagtgg tggtatgcac
    13921 atactcttta aaaaacgcga tgatattaat tacacgcaaa aaataggagt aatgcctggt
    13981 gttgacataa aagcgaatga taataatttt gtcaaaatat tcccatctga cggacgtgaa
    14041 gttttacaag cagttaaaac attaccttat tatgatggaa aatttgaaga ggaagtattt
    14101 aaaccaaaac aagaaggtat aacaacctat tttggatgtt ctgtcacata tacatcaaac
    14161 ggaagtcacg aaggccgaga ggcttatgaa cgtgtagcaa ctggaacatc atataacagg
    14221 aatgatgatt tgtttaaagg agcgtgttgg gcgtttgaaa atggtatcga tattgatgat
    14281 ttaacatcaa ttattgggac ggttaaaggc agagacgtat ttacgagaga ggagtttgaa
    14341 ttaacaattg aatcagcgaa acgaaaagtc agctacgtca ctatcagaac atgatatcca
    14401 aaaccttatc cgaatggaat tatcacaagc ggggtatatg gtatttcgag caaatgtcgg
    14461 aaaggtgaaa acagcagatg gtagattttt tgatacaggt ttaccaaaag gtttttgtga
    14521 tttgtttgga ttcaaaccaa acggacaaat attttttatc gaagttaaaa atgaaacagg
    14581 tcggataaga ccagaacaga aaaactttat ggaggttatg gcatcgaaag gggcgttagc
    14641 cggagtggcg cgttcggtgg aagatgcttt gaaaatagta aatggatatc attaataaat
    14701 ttaaaaagtt agatcattac aaggaaatta agcgattgat gtacgcagca atgactttct
    14761 gaggaggtat agtatggcag ataaaataaa cgcagagagt atgcaagctg catacaacga
    14821 aaattatcaa atgtttttag ctaaaaatgc agattatggg aactcgttcg aaaagtcttt
    14881 aaatgacttc ggatttattg ctggtatcgt acgtataggc gataaataca acagactata
    14941 taatcttata agcagcgaca aaaacgtctc agaaagcctg tcagacacgt taaacgacat
    15001 ggctaattat tgcgtgatgt tagcggtttg gttggaggaa gaggaacgac accgaaattt
    15061 agagcatggg ggataaccga tgttaattaa taaaaattta gtaagagaca ttagacttgc
    15121 tatcaatgaa tacgatgata attatgacga agataacgca gaagtttgta tagaagtcat
    15181 tagacgattg attgaacagg aggaattctg atgataccga attttagagc gtttaataaa
    15241 gagaccaaaa agatgtatgg tgttgacggc tttgaattaa gtgtgcgcaa aatatataga
    15301 tgcagcttag cagatgatga gtttcgttgt ggtcgcttag agacgtttca ttttgtcgag
    15361 gataactttg acgattatat cctcatgcaa tcaacaggcc taaaagataa aaacggcatt
    15421 gaggtgtttg atggggatat cgttaagtgt tgcagacttt ttaacgactc tttgtctgag
    15481 tacgtaggac aagtaaaatt tgtagatttt ggttggaaca tagttgataa agcagataca
    15541 cacgatccat tttacaacta caaagacggg tgtccagacg aaattcggga aatcgaaaat
    15601 atcggaaata tacacgaaaa tccagaatta ctagaaaggt tagaaggatg attgaagaac
    15661 aagaaatact tgactgcatt acgcaggaca caaaagcaac ttaaacgtgc tagcgagcaa
    15721 aatcagagac agacaaagcg gattgctgat tttgactgga aatgggggat agggatgtca
    15781 gacataaaaa ttttagatgc ttgttgcgga agccgcctat tttggtttga taaaaatgaa
    15841 gaacacacca cttttatgga tattagacaa gaaacatttg atattcacgg aaaacatgta
    15901 aatgttaaac ctgatgtttt gggagatttc agaaatatgc catttgaaaa taatatattt
    15961 aacttagtgg tcttcgaccc accacactta aaatatgttg gccaaaagtc aattatgaaa
    16021 gcgcaatacg ggcaacttga taaagacaat tggaaagaag atatttcaaa aggatttgaa
    16081 gaatgcatga gagttctaaa agtaggtgga actttagttt ttaaatggtc tgattgtcag
    16141 ataaatgtaa aagaagtttt atcagcaatt ccgtttacac cactttttgg gcagcgacga
    16201 ggaacaactc actggatgac atttgtaaag tttgcagaat tgactgggaa tggaggatga
    16261 ggatgtcatt aattgatgaa gtaaaagaat gcggttcaga atcacacaaa aaatggtttg
    16321 atagattttt tgatgattta aaaatcgagg agaagcttaa acaatctgcg atgaaaggtt
    16381 tctctggcta taaaatcgct atccccaagg acgatgaata cttagctaga cgacttgaca
    16441 gtcaaaaaac aatagattta ctaaaacaac ggctagggga cggttttgat gttgaaataa
    16501 caaatcttga atcgtcgtat aaaatatttg gtaaaccagt tgtgttaagt agagatttgt
    16561 gtatttattg gacgaatgga ggataagatg gaaaattatt tatgttactg ggaaaaaaga
    16621 ggacaaaact ggtatttctt ggctaactgc gaagactcac caactggaag aatatttaat
    16681 cttactggtg ttaaattcaa ttattgtcct ttctgtggac gtagattaaa agaggtgaca
    16741 aatgaaagtt aaagatttac ataaagtgct agctaaagtt gacccagaac ttgaagtgtg
    16801 gataaacgaa aaagacgaag gagttttaac taaagctaaa aacgtaatga cttggaacaa
    16861 gcatcctgat aagtttttta tcaatgcaga aacatatcca gaatagaatg aggtgacgaa
    16921 atgaacattg aagaagcgaa aaaaatagtg gacaaattgt cagtagatag tgatgagctt
    16981 tggaagattc caatgattcc agcccataag gtaaaagcct tgcttgacac acttgaccaa
    17041 ccaaaaccag aagtgccaca aatgatattt gatgtgatta aaagctttga tgatgatgta
    17101 gattatttac atcaacacat gagtcgacaa tctgatgaag ttagagagtg gctaactcac
    17161 aatgaacgtg agttttatga agcttggcta gcttatccaa atatcacaat tgaaaaagag
    17221 aagctttata cagttgagat acctaatccg aatgaaagac agttaagttt tgtgctgatg
    17281 agacaactta gcggaaatgt aagtatcaaa gttatgcata gagataactt agacttacta
    17341 aagacagata atgatttaca actaacagaa tccgaaatcc gcaaagattt cgactgggcg
    17401 tggcagttta gagaagaggt ggcggaatga aagaaaaaac aatttttata tcaaaagaat
    17461 atgcaaatga ctttaacaat gacaaatata atttgtcctc tggctattat tttagaaatg
    17521 gtaaaaaaca tgatattgct attgttaaat atggtgaaaa agattattta aaaaatactg
    17581 atttagcata tgttgtatgc gataaaatcg ttgatgcaga ctctataggc ttcgtttatc
    17641 atggtgaata tgaaacttgg cattttaaac tattaaacac agaagcaaat taaagtccca
    17701 cgcaagcgcc taagagcctg caatggctct gtgggtctac gagctggaat actcgttaaa
    17761 cttaccctag aagctttctg taagtattca gctacgtagc gtggaataat cgttacgtag
    17821 ttatagagcg aaatttttag aaagggaaat atcctccgac attttttcat aaaaatctaa
    17881 agtctgttat cgctcacaga tgattataca agcgcaatgc tgcaaataaa gtgctgacgc
    17941 aaaactaaaa atttaatact cgacaattta acaacaaaaa taagtcagca gaggaaggaa
    18001 aggagaacaa taaaaaagcc ggctcacgca agccagctcc gatatatttg tctactaata
    18061 ttatatcata cgaggagctt tcatgacgtt ttttccagaa attaatatac aaaaaactaa
    18121 atcaaacgct aagcgaaaac taagagagta tccacgctgg cgtaggatcg ctaatgatgt
    18181 agatactcaa aaagtgacag ccacttattc ctttgaacct agacaatcac atggaacccc
    18241 tagtaagccg gttgaacgct tagcactcaa tcgtgtgtca gcggaacaag aattagaagc
    18301 aattgagcaa tcggttagta tgatactaga gccagaaaag cgcaggattt tgtatgacaa
    18361 atacttatct ccttacaaga atgcagataa ggttatttat acagaattat gtatgtcaga
    18421 gagcttttat tatgacacgc tagatgctgc attattagct tttgcagagc tttataggga
    18481 gggctctttg attgtagagc aaggagtttt tgactagttt ttatacagta atacaatagt
    18541 ttatacataa aaatatgtgt taatatagta ttatcaaaat agcaagaaga gataatcatt
    18601 taccaacagg ctatttattt agtcgtcaac tttaactact atcgaacttg ctattttgta
    18661 gctaaaaggc gagatagggt gttgagacgt agctcagttg ggggagcgat atgactataa
    18721 agggtctgga acgtacgcag gttcgaatcc tgccgtctca atcagcgtca gaggagacgc
    18781 gacccagtat cccgaaaggc agtcacgcac tgatatactg gtttttactt ggttgatagt
    18841 catattccta ccaatgacga ttggtaaaaa agatacatag atcggctgta tagtctacgt
    18901 gtacgtattc acaacagatt gactcatttg attaaccaca agtaaaacaa gggtcgcaac
    18961 cttgcttgtg gttagtaggg acaatggttg taagtcggtt cgattccgac tgttcctgtt
    19021 cgataaatag aagtgtccca gaatggggta ggcaataggc ttagcattca tttgctattt
    19081 atctatggtt aaccaattag tcatcacatt gtggtggctt tttattatgg aggttagggt
    19141 atgaggccac agaagttaac catcgctggt ggtagacgta caacggttga ttatgatgat
    19201 aggtcagcag agtatcgcga ctataatcgc aatcgctgga agtacgataa gcaagtcaaa
    19261 cagttttata actctaagtt gtggagagag acaagtaagc aagtcttgtt gcaaaacgat
    19321 tatgtctgtg ctatgtgcgg tggagaagcg actatgactg accacatagt ctcagttaag
    19381 aaagattgga acagaagatt agattggaat aacttgcaag caagctgtaa agcgtgtaac
    19441 gatagtaaag cgataagaga aagatgtaaa aataattaat tggaaaacgt gcgtaaaatt
    19501 aacaacagca cgttaatgtt cggaaaatac cccgcttgtt tttgaacagg gctatatcgt
    19561 tcgtgttttt gagaacgcgc ccttttccgt gcaaaatatt ccctttttga aatttttgaa
    19621 ctgtcaattt tcgtgtaaag gaggtaactt tgggaagaaa attaaaggtg gttgaaacaa
    19681 caaaaaaaca cctcacaaaa gaagaaaaaa tagcaagaga aaccgcgcag aataaggctt
    19741 ctgacggact tgataaatta caaataacgc cccctcggca tctcaatgag gtggctagag
    19801 cggaatatcg tagaatcata aatgacctcc agaccctacc cgtaagaaac ctcgatagag
    19861 gccttctaga actctattgt tcttggtatg caatctataa agagacaacc aagaaattag
    19921 atgaaattgg ttattttaca aacgacccgg ataaagggtt gattccaagt ccgctcattt
    19981 taacattgga aaaagctact gcgaatatca gaagtagcgc tagtcaatta gggctgacag
    20041 tagatagtcg tatgaagatg tttataccta aagaagaaga aaaacaaaaa agtatatttg
    20101 ataaatttgg cggttagtta gaaaggaggt catgacaata gaatacgatt attcagcaat
    20161 tagcgacatt tataaagatg acacttttta ttatgcaaaa aagattgttg atgaagagat
    20221 taaagcaagc aagaaagtgt ttaaggcttg tttaagacat ttgaatgacc tcaaacgtat
    20281 agaagatgaa gattttaaat tcatctactt gccacaaaaa gcatctgatc caattaattt
    20341 cattgagatt ttaccagacg tcaaaacagg aaaaccttat ccattagcaa tgttccaaaa
    20401 atttattatt gggaatttat atggatggcg gaagaaaaca gaccattcct taagacgttt
    20461 tagaaaagct atgatttctg ttgctcgtaa aaatggtaaa accattctta tagctggtat
    20521 attgctttat gaatttttgt ttggtcacaa tccgtctatg agtaggcaat tgttttgtac
    20581 agcgaacgac cgcacgcaag ctaaaatagc ttgggatatg gcaaaaaagc agttagcttc
    20641 cttaagagca aaggatgccg atgtcagaaa agctacaaag attgtccgtg atgaactaaa
    20701 aaacttacat gatgaatctt atataagggc gcttagtcgt gatactggcg cagttgatgg
    20761 atttgaaccg tacgttggag tgttggatga gttcgcagcg tcaaagacaa acgaaatgtt
    20821 agaactattg gaatctggtc aaggacagtt ggataatccg tttatcttaa tcatttcaac
    20881 ggctggtatg gatttgaatg ttccgatgca cacaattgag tatccataca ttactaaaat
    20941 actagacgga gaaatcacag acgagggcta ttttggctat gtcgcagagc aagacaacga
    21001 ggaggaaatt aaagatgaaa cgaattggat aaaatctaat ccaattctcg aagtcgatac
    21061 tctacatgat aagttgatgg actatctaag aactcgtcgt aaggtatctc tagagactgg
    21121 agaagtcaac aaagtgttga tcaaaaactt caatatgtgg cgtcaatcca gcgaagaatc
    21181 atatatagat aaacagtcgt gggagcttgc taagattgat aagccagaca catacaagcg
    21241 tagggtttgg ctaggtgttg acgttgggcg tgtaagtgac ttgtttgcca ttagtcctgt
    21301 tgttatgatg gatgattatt ggtatgttga tagtttttca tttgtagcta caaagtatgg
    21361 cttaactgcc aaagaaaagc gagatggtgt atcttatagc aatctagaac gtcaaggata
    21421 ttgcgaaata acaacccttg agagcggggt tatagatgat gaacgggttt tggaaaaaat
    21481 agaggagtta atctatataa acgaatggga agtacatggg atttgctttg acccatacca
    21541 attcggaaca ctacttacaa tgattgaaaa aagacatccg gaatggcctc taatagaagt
    21601 ttcgcaaacg acaatggtgt taaacatgcc gacaaaacaa tttcgtgacg accttaaaaa
    21661 aggcaaaata aagcattctg gcaatccact attgaccatg gctgctaaca acgcttatat
    21721 taaaaccgat aacaatggca tgaggattga caagaacaag aatagcaaca agattgaccc
    21781 gctagacgca gttcttgacg gttatgctgt atgttaccta gaaccatttg acggttctgg
    21841 ctactggaca agcgagaaaa ttttaggagg agagacgcta ttttgattgg ttttatttta
    21901 aaaaacatac atacattgat cttgctggta ggtttgggac tgttgatata cggtcttttt
    21961 ttatttggtg ataaggttgg ttttattgcc agcggaatta tattagttat tttagctatc
    22021 tatgtagata gtgtaggggg aagaaaatga acaaacgtat taagaaaaaa cgcaaactag
    22081 aacactatat cgcttcactc atcgcagaaa acgttatgtt ttcaaaggaa ttaatcaaac
    22141 aacatgaacg gattgaacaa cttgaaaaaa ttgtagagca taacgcacaa gcgactaaca
    22201 atgaacttag tcgcatcaaa aaacactcaa agaaaaaatg gaaaaataac gtcaccttac
    22261 ctatagaaag gaggtgagaa tctatgagtt tttttcaacc tttgggaact tcaaaggtat
    22321 cgtatgacga ttatatagcg tcagtcttag ctggtgacgt ctcacagcag tatctaggtg
    22381 tatcagcctt aaaaaatagc gatgtcttga cagctacgtc tattatagct ggtgatatag
    22441 ctcgttttcc gctcgttaaa aaggatataa acggggatat tatccacgat gaggatatta
    22501 actacctatt aaatgtaaag tccacaaaca acgcaagtgc taggacatgg aagtttgcca
    22561 tggctgttaa tgcaatttta acaggtaatt cgttttctcg tatcttgcga gaccctaaaa
    22621 ctggtcaagc tttacaattt cagttttata gaccgtctga aactactgtt gaagaaacag
    22681 aaaaccacga aatcatctat acatttacgg atactttgac tgctaaagag ataaaatgtt
    22741 ttgcacacga tgttattcac tggaaattct tcagccatga cacaatactc ggaaggtctc
    22801 cgctattatc gctaggcgat gagatagact tacaaacggg tggtatcaat accttaatta
    22861 aattcttcaa agatggtttt tctggtggta tcttaactat gaaaggtgct caattaagtg
    22921 gagaagcgcg tcaaagagca agacaagact ttgagaaaat gcgtgaggga tctgttggag
    22981 ggagtccact agtttttgac agcacaatgg aatatacgcc acttgaaatt gatactaacg
    23041 tattgcaatt aatcacaagt aataacttta gtacggctca aatagcaaaa gcgttgcgcg
    23101 tgccaagtta taagttgggc gtaaacagtc ctaatcagtc tgtagcgcaa ttaatggaag
    23161 attacgtaac aaatgacttg ccattctatt ttgacgctat aacgagcgaa atagggctta
    23221 aaacgcttaa cgatggagac aggcgactgt atcatgttga attcgataca cgaagcgtca
    23281 cgggtcgcaa tgtcgatgaa attgtcaaat tggttaataa ccaaattttg acacctaatc
    23341 aaggattggt tgaacttggt aagcaaaaat caaaagaccc taacatggat aggtaccagt
    23401 ctagtctcaa ttacgtgttc ttggataaaa aagaagaata tcaagatcaa gttggagtca
    23461 aagggaaaag gggtgaggta aatgccaaag aaaataaacc ttaaaggtcc actaattgct
    23521 aataattcac aagaagtcta tgactattat ggcatggagg ctgttagtgc taaaagcatt
    23581 attgacaaat tgccagaaga taatagcgac attatcttgg aagttaattc aaatggtggt
    23641 ctcgttactg ttggtagcga aatatatacc gctctgcgaa attacaaagg gaaagtaacc
    23701 gcagaaatca caggaatggc tgcgagtgca gcgtctgttg cagtgatggg ggctgataaa
    23761 gtcgtcatga gcccaacggc tcaaatgatg gtacataaag ctttgtttaa ttgggtttct
    23821 ggtaatagcg acgacttgga caaagcttct agcgctctta aatcaagcga taaagccatt
    23881 gttaatgctt atgttgcaaa aacaggtaaa tcagaagatg aaatcatgca attaatgcgg
    23941 aatgaaactt ttatgtcagc acaagatgct gttgagagcg gatttgcaga tgaagtgatg
    24001 tcgtttgaag ctatagcaag cattgacaat caaatgttgc cacaagcggt tattgatgac
    24061 tattacgcaa gcagaagcaa acgtaagcaa gagattaata acatgttgct agaaatcgaa
    24121 aaagaagaaa ttttacaagg gctataagct ctttttattg gaggaagaaa atgtttgacg
    24181 aaaaaatcga agaaattaaa gcggatatca ctgaattaaa caaagtgatt gctgctaaaa
    24241 ccgcagaagt caaagctgct ttagaatcag atgacttaga aactgctcgt tctgttaaag
    24301 cagaggtaga agctactaaa gcgaaattag ttgaatcaga aaacgacttg aaattatatg
    24361 agtctagcgt tgaagttggt ggtgctgaaa atattggagg aaaagaagtg acacaagagg
    24421 aaaaaacata tcgcgaaagt gtgaatgatt tcattcgttc gaaaggtaaa atcgtaaacg
    24481 attctttacg ttttgaaggc aaagatgagg tacttatgcc tattaatgag acaacccccg
    24541 tagaacctaa aaaagacggt gttaagaaaa cagatgtaaa acctgtctct agtgaagaaa
    24601 tcctttacac accagctcgt gaagttaaga cagttgttga tttgaaacaa ttcacaagca
    24661 tccaccaagc taaaaaagca tctgggaaat ggccagtttt gcaacgtgcg actgaaaaaa
    24721 tggttagcgt tgaagaatta gaaaaaaatc caaaactagg taagccacaa ttcacaaacg
    24781 tcgcatggga aattaaaact taccgcggag ctatcccgtt gtctcaagaa tcaattgacg
    24841 atgcagacgt agacttggtt ggtattgttg ctgagacaat cggtcaaatg aaagttaata
    24901 caacaaacga cgctatcgca acagttctca aggaatttga agctaagaca gtaaaaaact
    24961 tggatgaaat caagaagctt ctcaacgttg aattagatcc tgcttacaat gtgtcattta
    25021 ttgtatctca aagcttttat caaacaatgg atacattaaa agataagaat ggtcgttacc
    25081 tacttcaaga ttcaatcact tctgtttctg ggaaagtatt cctcggaaaa cctgtttttg
    25141 tgctagctga tgaggtttta ggtaaagata cagcatttgt cggagatttc aaacgtggtg
    25201 tattattcgc cgaccgcaaa gaccttggac ttcgttgggc agataacgaa atctacggtc
    25261 aatacttgca agcagttctt cgctttggcg tttcaaaagt tgatgacaaa gctggctact
    25321 atgtgacatt caaaccagaa caattgccct cataaggcca ctcagggagt ggcgcaacca
    25381 gaagtaagca gtgctacagt tgttggtaaa ccaaccaaag acagcaccat tgaagatatt
    25441 aaaaaatatc taacaagcca aaatattgac tttagcggta agacgctaaa agcagacttg
    25501 ttaaaactgg ctggcgttga agaggtatag ttatgactgt atctaaagag ctgctagata
    25561 gtgtgaaact ctattgtaag atagattttg attttgaaga tgacattatt gaggaaatga
    25621 ttgcatcagc acaagaacaa atatgttttg caatagaaga aggctctact ccagaaatgt
    25681 ttaaaaacta cgctaagttt tccttagcgg ttaaaaaaca ggtaaaggaa gagtatgatc
    25741 atcgtgggtt atcggcggat actaatcgtt atccgcttgc caacggtgtt ttgaacatta
    25801 tccatcaatt gagattgagg ggtgatgggg cgtgattaca cggaaaatga atgttagaat
    25861 agccattttt agccaaactg gtggtcagaa tgaagatggt gaagttgtat ctgctatcag
    25921 gaaagacttg tatacctgct gggcagaggt gtctaaaact caattgagag attttaatta
    25981 tcaatcaaga tatcagaatg ctagcaatct cccaacaaat aaagatacaa aagtattttt
    26041 aattagatac aatcctaagt tgtctattga taatacgatg tttattgagc ataacaaaca
    26101 tatctacaaa atagataaaa ttgaatttga tgaatccggc aaggaaatga taatattgag
    26161 tggggttagc atatcatgac aaaagggctt gatgaaattt tggctaatat tactaagtta
    26221 gaagtaaaat cgcccaaaat tgccaaagaa gcggtaacac aggttgctga agaatttgaa
    26281 aaagcactta ctaagaatac gccagtcgat tattctgtgc atgacacaaa actcaaatat
    26341 gatactgcaa ttagtggatt caaaggtgct aatgtaggta taacgtcaaa aaaaattgga
    26401 tatggacgaa aaacgggctg gcgtgctcat tatccaaaca atgggacgat ttaccaaaaa
    26461 ggtcaagact ttaaagaaaa aaccatcaac gaaatgaccc ctagagcaaa agaaatttat
    26521 gctaaaaaaa tcaaggaggg actagggctt tgatagctga aacaactgct tacaaattgt
    26581 taaaaaacga taggacgctt aatgagctgt tggataagct tagaggcggt ccttttaaaa
    26641 acggatttga tcagggtatc tttacatatg atattcctga caatcccgtt gatttaagaa
    26701 aagtagaact agcgccattt atgcgtataa atacaacgta tgatggtccg tccctatatg
    26761 cagatgatga catgatcagt aatgaacagc gtattacaat caatttttgg tgtaaaaccg
    26821 cttctcagtc tgaccagatt gccagctgta tagataggat tttaaaaaat ggcggttttg
    26881 aaagatacac cgcaaacgaa aaaccaaggt ataaagatag cgatattgac ttactcatga
    26941 atgtaaggaa ataccgctat tttgattttt attaagaaaa gaggaactaa atgggaaaag
    27001 taaaatttgg actacgtgac ttccagtatg ctgtgcttgg ggataatgat aaagttaatg
    27061 aaaaagacaa aggaatcaaa gctctacccg gcatgaaatc agctaaactt gatattacaa
    27121 acgagcttgt aactgtcatg gctgatgatg gaccatatgt tgttttatct ggtggtatca
    27181 cagaaacaaa attggaaatc gaagtccttg acttgacttc cgaagcgcgt caagatttct
    27241 tcggaattaa agctgacaaa ggtattgaaa gatacaataa atcgctcaca cctaacaacg
    27301 tcgcttgcat gtttagaaca agtgatgaaa atgggaaagc tatctgggtt ggtttgttaa
    27361 aaggtaaatt taacattcct ggcatggata ccaaaacaaa agaaggtgcg cctaacccag
    27421 aaccagacaa agtaacaggt aactttgttg ctcgtggaac tgatggtgat gtagttgtca
    27481 tcggtcgtga aggagtgaat ggatttgagc taaaaaaatt caaagaactt gtgtttgatg
    27541 gggtgacagg attggaaaaa tcatcagaag acaatagtct aatgtaacac ttaaggttgg
    27601 gttttaaatc cagcctttat tttttgataa ggagcaataa tgtacgaaat tacacttaaa
    27661 aaaggcggag tagataagac tttctcaaaa gattttgtca atgtcgaaga taatatgttg
    27721 gcggttgagc atcaagttag acagagcgct atctttagtg atgataagcg ccggttagac
    27781 gataaagaac atcgcaaatt aaacgaatca tatttacaga tgtttgtaga aatgtacgga
    27841 aatcaattca atgttgacga tttgaaacag tctaatgtgt ctgttttgga caaattaact
    27901 gagctttttg ttgatgcttt aggcggagag aaagaggaag acgaaaaaaa ggaacaataa
    27961 cgcctcaaga agctaaagac aacctgctct tgtggattca aagtttacta aaaaatgggt
    28021 ataccatttt agatattaaa aaaatgcgct tatcagacat agagttgatg gtaaaagcgc
    28081 tagaaactga ttttgctgaa aaagaagaag tggttgaaac aactttagac aaagcgttcc
    28141 cattcctttt cggctagaaa ggagaattaa tggggaatat aggtgattta gtagcgactg
    28201 ctacactaga catatcacct tttatgtcta atacaagaaa tttaaaaact tatatgaaga
    28261 ccttagataa ctctctaaag gctgtagaaa atagttttaa tggtcatggt agtagagtta
    28321 aaggtttaaa agctatctac acagaaactg gcggtgcatt aaaaagttat caagaattac
    28381 taaaaaaaca atctcaaaaa tataatgagt taaaagaaaa tattggcgat gtcaacaagg
    28441 cgacagcaag ccaaaaacaa gcacttatta atgctaagtc agcgatgatg gaaacagctg
    28501 ctaaagtttc agaattgcaa agtaggctca aagctttagg cgctgaaaca agtgttttta
    28561 ctcgttttgg taacgctgcg gagcaagctg ggaaaaaaat gaagtcattt ggcgattctg
    28621 tatctggtgt tggtgctgct tttacaaaag gagtaactgc accaattgtt gctggcgctg
    28681 gatatgctct taaggctgct gttgattatg aagatgcttt tgctggcgtc aaaaagacag
    28741 tagacgaagt aaaagactca aatggtaaag ttgtctactc ttacgatatg ttgtccaaag
    28801 gaattagaca aatggctaaa gagataccag cttctacaac tgaaatttct cacgttgctg
    28861 aagcagctgg tcaattaggt atcaagacaa aggatgtctt aggatttact cgtgtcatga
    28921 ttgatatggg gaaatcgact aacttatcat cagaagaagc ggcaactgca ctagctcgtt
    28981 ttgctaatat cacgcagttg gacccatcaa aatacagtaa tctaggaagt tctatcgttg
    29041 agttaggaaa taactttgcg acaactgaaa aagaaattgt tgaaatgggt cttcgcttag
    29101 ctggcactgg taaagttgta ggattgacag accctcaaat attaggtctt gcaactgcta
    29161 tgagttcggt tggtatcgag gcagaggctg gtggttcggc gtttagtcgt gtcatgcaaa
    29221 aaataaacac acaggttctt tctggtggag aagatttatg gaagtttgcg aaaatagccg
    29281 gcaagtctgc tgatgaattc gctgcgtcat ggaagaaaaa tccacaagaa gcgattattg
    29341 attttgtaaa aggtcttaaa cgctttaaag aagaagggaa agacgtaact gctcacttgc
    29401 aagacatgga tatcaattct gtacgtgaaa ttgatacatt acaacgtttg gcaggagctg
    29461 gcgacttgct aggtgatgct ttcaagtctg ctaataaagg ttttagcgaa aacaaagctt
    29521 taatgaacga agcgaacaaa cgttatgaaa cgacagcatc gaaattacaa atgctaaaaa
    29581 atcaagttaa tgatgtcgct attgaatttg gtggacctct tattgatgca ctgcgtgacg
    29641 gtttagaagc tggtaaacct attattaaag gcgttgcaga cttagcaaaa gcatttagtt
    29701 ctctggataa agagcaacaa caacaaatca taaaatgggg actaattgca gcagcaactg
    29761 gacccgcatt gtcaatcttc ggcaaaggcg ttggcgtaat tggtggaacg attcaaggac
    29821 taggaaaatt aagcaaaacg ttaggcacac tctccgggag tctgggagca gcaaaaactg
    29881 gagctgctgc tttaggagtt ggagcggaag gtgcaactac tgcattaact ggaatgagtg
    29941 gagcggcagc attgctaggg aatcccgtaa cttggggtgt tgtgctagga ggagctgctg
    30001 cgcttgctgt cggatacttt gctcaaaaag cctacgaagc gcaccaacgt acgcaagagt
    30061 gggggactaa agttagtcaa gtacaagcca atgaactgca atcctttaaa gataaggttg
    30121 atcaaacaaa tcaatcaatg gaaggcttta gagggggagc tgaacaggtt aactccgtga
    30181 agacagcgtt ccaaggttta gttgctgaaa ttgaaaagct agaaaataaa gatttaagcg
    30241 aaaaaatcaa acttgcagaa caatttggtt ttagccaagg tacgatggac caaataaaaa
    30301 aatctagccg acaaactgta gagaatgtta aacagatgtc tgatgaagtt atcaatatct
    30361 atcaaaatgc tagtaatgaa cacagaagat taaccgaaga agaaaaagct gttgtattag
    30421 cgaatcaaaa cgagcttatc aatgtgcaac tgtcaaaatt gaactactct gctaaagaga
    30481 aaaaagctat cgtaaaagca atgaatggcg atttaaattc attgaatagc cagcaattaa
    30541 ccaaagctct tgaagttact gaaaaatgga taaaagcaga aaataagtca tacaataaac
    30601 tcaaaagtgg tcttaaaaaa gtttacgact ctataaaagg taatgacaaa gaggctgtca
    30661 aaactcgcga agagatacac aagaagcaac aacaacttga agcagaccat tacttaaaaa
    30721 tggaagctta cggcaagcgc tacgctgcaa ttcaaaagaa attgctaaaa ggtactgcta
    30781 aatatttaga ccctcaattg caacaagcga tggttaatga tgttaaaaag caaatgaagg
    30841 agcttggttt gtcttacgaa gagttgatga caaagacaac caaagcggct tcaaaagcgc
    30901 aagaagtgaa tacaatgtgg gctagaacca ctaagaaatc tacagaagac caaaagcttg
    30961 ctaattcgca gtggaacgga cttgtctgga atcccaaaac aggtaagtta aaaaccaatg
    31021 ctaaagagga agtagccaag gctcttgaag cggaaggtgg ctgggacaga cttaagttta
    31081 ttgctaaaaa cgcaaaccta gaaacaaacg ctcggataac catggcagaa gtacttgtag
    31141 agactggcaa atgggatacc cttactccag aagagaaaga gttagttgtt gatgggcatc
    31201 aaggtattca agctatagta gaaagtaagc aacatttaga aatatggaat agtttgccag
    31261 aagaagttaa gcggatttta ggggataaca aagacttcct gaataaaaaa ggagttgcaa
    31321 cacgaactct tgaaaattgg aatgctttaa aaccagacga aaaaaaatta ctcgctaaaa
    31381 acttaacaaa aaaagggaaa gatgaagcac aaaaaacgat caatagctta gtcggaaaag
    31441 aagtcaaagt gacagcggct aataaaacat tgtccggagt taacagcgct caaagaacat
    31501 taaatagtgt gcaagataaa catgtcacta tttgggcgtc aatcaaaaaa actgcgagtg
    31561 acttatggag caaactaaca ggatacgctg ttggtacaga ctaccaccct ggtggacttg
    31621 cgatggtcaa cgaccaaaaa ggttctttat ataaagagtt agtaacatta ccaaacggac
    31681 aatcttttat cccagatgga cgtgacgtta tcttgccact ccctcgtggt tcgaaagtca
    31741 tgaaagcaag tgctactcgt gattacatgt atgacttagg aataccaaaa tatgcaaacg
    31801 gtattggttt tgacaacact aagattgcta atatcacgca acgaatgagc gaacttccta
    31861 aaaacacagt gacttcaaca acagatgata agttgtattg gatgattgag gaaatgattg
    31921 cagttttaaa atctactaaa gataacagcg ttattgagca agctttagac attgcagaac
    31981 aagcaataga gcgtccagta gaactttact tacaggacgg tcagtgggta gctaaagttg
    32041 cggaccgcat tacaaattat caaacgcaac gaaatagtcg caataatcga atgaaaggaa
    32101 tgctaaattg aaatttattt atgacggtgt agacatgtct aaatttttta ggatatctcg
    32161 tgttgagcgg tctataggaa atgaacgaac attgtcgttg aatgagacgt ttcaacttgg
    32221 tacagctatt cgtggcataa agacgggcgc taaaattatc aaagtacata tagagccact
    32281 agaaataaat ggcgttttaa ccgagcaatt aaaacatgaa cttgctggtg tgttaaatgt
    32341 tgacaagcct aaaaaaatga cgttcggaga tgaaccagat aagtactatc tcgggcttgc
    32401 acaaggtgag atttcgacag aaaaagtagc aaggtggtat cagagggctg ttatcacttt
    32461 ttacataccc gacggtgttg cacactctat cacttataaa aagttttcgg actatacgca
    32521 agaaggcaac aagcttattt ttaatttgca aaatgatggt aatactaacg cttatccaat
    32581 aatcaaaatt aaacataact ctgaaaacgg ttatatcggt atcgcaaatg aaacgggcgc
    32641 ttttgcactt ggatcatcgg aggaagaaga tgggactatc gttcatcgca acgaatccct
    32701 ttttgattac tcaaaagcta ttgcacaagc tttagaaggt gcgcccaacg tcgcaaaact
    32761 caactatatg ccaccaacat atgacacaga acttaaacgg atgcgcattg ataatatctt
    32821 aggctctggt aaaggcggtg aatatgttgt tattggaaat agagggacta ccccgggcta
    32881 caccgagcac attgggactc gaacgtttat tatcaatcct gattcaaacg gagaatacac
    32941 tctcaatgag cacctatggt ggcaacagat ttttattgct actgcgcagg atcagaaagg
    33001 gtttttaaag ctttgtgtaa cgggaatcga cgatgaagga aatgacgagt ttttgtatgg
    33061 aatcgaaact tacaaacgaa aaaatggttt tgaaacagaa tacaattttt ttgctcttga
    33121 tgatgacggt gtaggctgga gattttataa gcagtttaaa ttccaagcag acagaaatta
    33181 tcacaatcct ttttcaatga atagaagtag agcggttgag attttcaggg aagaagacaa
    33241 gtttcgtatt tattttaacg gtgcgcatca tcatgtaact gttccatctc ttaaaggaaa
    33301 aaaatctcgc aagatacatc ttgcaatggg gacatgtagt gatagctcta aatatatcaa
    33361 ctacaatctt ttcgaaaagg tcaactttga aaaaatggga gtgtctcact acaacaacat
    33421 tgttaacaag tatcaaccag gcgatgaagt ggttatcaat tttgaaaatg acacagtcaa
    33481 aacaaaagac ttagattcta ttcaagacat ggttttaggt tcgcagccta tatctatacc
    33541 acccggaaaa acagagttcg tcatgcagct atctaaattt tctcaatatg cgccaaacgt
    33601 tgaaatatta atggaagaga ggtggttata ataacgctag taatacatga ttctaagttg
    33661 caccctgttt tgttgctaga caatgaaaaa caaggtacgc ttaattattt tgacgacaca
    33721 tggactagac agttaactac aggctcatct gtttttgagt tttctgtgta caaaaaaaca
    33781 cttgaaggag acaacccact caatcataag tatcaagtgc ttaatgacca agcgtttgta
    33841 tcgtttgtac ataaagataa agtacagctc tttaatatca tgcagatcga agagactgaa
    33901 acgactgtac gttgctattg cgaaaatctt aatttagagt tactaaacga gtattgcaac
    33961 gcatataaag caactaaagc aatgtcattt gaagagtatc ttgtgcagtt tgatatttta
    34021 aattggggtg ctttgacaat tggcacaaac gaagttaagg acaaaaaact gacattggaa
    34081 tggactggtc aagacactaa gttagctcgt attttgtcaa ttgctaataa ttttgatgca
    34141 gaaatcgaat ttgaaactca attacacaac aatcacacgt ttaaagcgtt tattgtaaat
    34201 gtgtacaagg aatacgaaga gggcgtgtcc tatggcgtag gtcgtgaccg cagcgacata
    34261 gtgttgagat atcaaaaaaa tgtaactggt atcactaaaa aattagacaa gcgccagatt
    34321 tacaacgcca tacgcccgta tggcaaaaag acagtcaaag gcgagcgcgt tatttctaat
    34381 cctgtaactc gcaaagtcac taaaacagtt gggtcaaatc gtacatattt aggcggagac
    34441 ctcaaatatt atggtcatac aatcaaaaaa gctaacgtac aatctattat taactacgcg
    34501 gtgcagtata atattttgcc gagtggaatc atatgtcaac tgtacttaga aagtctttgg
    34561 ggtgattcag cagttggtaa acgtgacaat aactgggcag gtataagcgg cggagcacag
    34621 acacgcccta gtggagtaaa agtcactact ggaatggctc gtcctcccag cgagggtgga
    34681 acatacatgc actacgcaag tgttgatgac tttttaaaag attatactta tcttttagct
    34741 aaacaaggac tatataacgt tgttggcaaa aagaatatag cagactatac aaaaggtttg
    34801 tttcgtgtcg gtggcgctaa agatgattac gcggcagcag gatatcaaag ctacacaaat
    34861 ttgatgacta atatccgaaa tggtatcaat aaagtaactg gaaatatcct caatacgatt
    34921 gataagctgt ggcaaacacc agtacagcct ataacagccg taaacgtagc tagaagagct
    34981 actaagacaa tgcaagcact aaatgaagct actagactta aaggtcgcag aatcggctca
    35041 ggacagtgtt atgctttgtc tggttggtac gctaagaagt tagacggagc ttggattgat
    35101 agttccatcg gtggtatccg tggtcgcatt ggcggtggga tggctgctgc tttaatcggt
    35161 actgactata actggggggc gtatggttgg aagctagaca ggtcgcctaa tgctggcaac
    35221 ttgcaagctg gcggtatcta taatgttaaa gcaaattttg gtgctccgtt ttatacaaca
    35281 caatgggggc acacagggat tatcaagagt gtgtccaaga ccagagttac tgttttggag
    35341 caaaactttg ttggccgcat gtatgttgtc gaaaactcat atgacattaa ctctttcgca
    35401 tctggattgc agacagtatg ctatcctcgt gagattgcac aaggaatggc tgtcaacggt
    35461 gcaacaacac agcaagttag tggtgggaca cagatatcat atgaggaagt cgtacaagaa
    35521 gcacagacag aatcatacga agaagaacaa atcatctaca ttgacaactc aatctacaaa
    35581 gagtggaaag atgaaaacgg taaagtagag tactatctca aaaatggatt tttgtacgca
    35641 cctttatcca gagaccgtta tccatctgtt ttaacaggta atgagacacg ggataactgg
    35701 atacgaaagg acatggaaat tgagactgat agtcaagacg tcttgatgtc aacaggtcta
    35761 aaagacttaa aagcacacgc ctatccagca atcacttatg aagttgatgg ctatgttgat
    35821 ttagaaattg gcgatgtcgt acgcattcaa gatgatggtt atcaaccacc tttgattttg
    35881 actgctcgtg ttattgagca ggttttgtct aaagataatc caaactcaaa caaaactaaa
    35941 tttagtaatt ttgttgagaa agaaagtcaa ctagcgtctg atttaatcag cgatatgttg
    36001 cgtctatacg atgagtcaat tccgtatgaa attaaactag cgacttcgaa cggtgtcgct
    36061 tttaaaaatg gcactggtga atctgtctta actccaagtt tgcaaaaaaa cggaaaagac
    36121 tatgaagctg tttattttta caaaaatggc gactcattaa ttgatatcgg accatcacta
    36181 attgttaaag caagcgactt taaccacgtt ttaaacgtaa cagtagaggc gtatttaaat
    36241 gaggagcttg tggcaagtac acaaatatca tttacagaca cagaagacgg tgcagacggg
    36301 aatacgcctt actggcatgt tgcttatgca aatagcgcag atgggacagt tgattttagc
    36361 gtgtctgata gcgcaaacaa gcgctacatt gggcaatata ctgactacga tgcaataggc
    36421 tcaaacgacc ctaaaaaata taagtggact gatatggttg ggacggtcgt cgttgggaca
    36481 aataatctaa tcgatggcac taagtcgttt agcggagaag actggtttac ttctgcaaca
    36541 ctagaagatg aaaatatctc taattatcca tttacattta aaaaatggat aagcggtcaa
    36601 aaggtatcac acgcaaaaga cattatggtt gagcaaggtg tgacatacac ttttagtgct
    36661 tatgttaaac gtgaggtagc tgggaattta tatttttatc tttatgatat agcagatggt
    36721 tttattacta gcgatacccc acgagagaca attataaaaa acgttgactc tagtctcaga
    36781 cgttttgaaa tcacttttac accaactaag acaggtagga ttagaccaag gttcgcgatg
    36841 gtgtcatcgg agcaaggtag tttcagctct ggtgggttta tgctcgttag gggaaataaa
    36901 acaggcgact ggcaggaatc agaagctgac aaagctagta atttagatgc aaaagccgat
    36961 ggtgctttta cagttgagca actaaatgca ctcgcagaac gtgctcgcat tgcagaagct
    37021 gaattgcaat ctaaagcgac actagacaca gtaaacgact gggttaaagc actacaagac
    37081 gaaatcaaag cacgagaaga aggacaaaag ctatcagaac aaaaactgat agactcttct
    37141 aatcgcatga tagcagtaca gcaaacgatt ggtgaaatgc aggttcgcac cgattttgtc
    37201 aataaattta tgagtcagtc ggaagatggg cttgttattg gtcaaaaaga cggtacttct
    37261 agtgttcgtg ttgataacga ccgtataagc ttttactcaa gtggtaaaga agtcgcttat
    37321 attgctcaaa gtgtgttagt tattgatagt ggtatcttta cgaccaaact acaaataggg
    37381 cgctatcgta ttgagcaata cgagctaaat ccagatataa atgttgtccg ctatgttgga
    37441 taggaaggag gttaaatgac aacttattat agtaactctg ataggagtta ccgcttaact
    37501 tatattgttg acgaggtctc aacatcaatc gcagataata gcagtcaagt tagatttaga
    37561 ctttatttaa catcaaggac taacagttac gcacaataca actttagcgg atatgcttgg
    37621 gttggtggta gatatgactt tagcgcccca tctgcgcttg gctttaacag taattattta
    37681 ctgattgata aaactatcaa agtaccacac gatgctgacg gtagtaagac tgtggttgtc
    37741 gcagctaagt tgtcaggtcc tggcgggtat gcacccggaa cgctaaatat accagaccaa
    37801 aaatttacgc tgactaaaat cccacgaaca agcacagttg ctgtaagtag tggttatttt
    37861 ggtgatacgc taaatgtcaa catcaataaa acaaatgaca gctttacata cgatgtcaga
    37921 tataacgtta acgggataac tggtactgtg gctagtgata tatcaggatc aacgactttt
    37981 aaaactagtt tggattgggc tagtacgatt ccaaatacaa ctagtacacc agctacaatt
    38041 tacgttgaca ctaaatcgaa tgggtcggtc attgggacat caacaggtat tttttatcta
    38101 actgtacctg ataacatcaa gcctaaaatt tccagcctca ctttatcaga cacgaatcaa
    38161 aaagcatcta caattgtagg tgctaataat tttgtccaga taatttctaa tccagtcgtc
    38221 acatttaatg gggctagtgg tgcttatggc tctacaatcc aaaattttaa cgctgaaatt
    38281 ataggtaaaa accaatctac gcagtcaaac ggtgggtcat taggcatttt aaactttagt
    38341 ggtaaagcca cagttaaagc aacagtaacg gacagtcgcg ggagggtgtc agaccctgtg
    38401 acaacagaaa tcaacgttat accatattcc ccaccagctt ttagttttac tgtaacacgc
    38461 gcaggcgcta aaaatgacca actagttgtc actcgtaacg ctaaaatctc cccgctgatt
    38521 gtcgatggcg tccaaaaaaa taaaatgacg ctgactttta aaacagcacc acttaatacc
    38581 acgagtttta caatagacac atcaaacgca agcggtactt atactacagt cgcagagttg
    38641 attaactcaa cagctacact tagtggttcg tatggagcta acaaatcatt tgatgtctac
    38701 ggtttgctta gtgatgtttt tagcgcaagt ggaggcggta cacctgttaa acaaacggta
    38761 tcaacagagt ctttcccgct gtcatggcat aaaaacagcg tcggaattgg aacattacca
    38821 aaaattgatg atacaggttc tttaaatgtc gcaggcaata tctattctga tggcaagcaa
    38881 atccaacaaa aacaacttgc tttaaataat ggtggggcat ttaggcatga tacaacagac
    38941 ttaaacagct tgcaagacac aggtttttat tgtgttttta aaggcgataa cagaccaagt
    39001 ggtgctggac cgggctatct aacagttgta agacacgaga cagccaatta cgcttaccag
    39061 catttttatg accgcacgaa caaaaccatt tttacacgag tgctagaaaa cggggcatgg
    39121 agtggttgga gtgagtacgc taaaaaagat agcttaccgc aatccgcacc agcggtagaa
    39181 gatactggtt ggcaatacat cggcaacggt tttaattaca ggaaaattgg tagcatggtc
    39241 actattaaat atgactttgc aacaaatgga ataaaccagt ttacggtcgg ttccatgcca
    39301 acgaatttaa ttccaaacga aatgatgttt gcggttactg cgtggactgt gcaattaaat
    39361 gtattaaatg tacaagttag tgcagatggt cgtattttat ggttcaaccc atcaaaatgg
    39421 gcggttaatg ttaaaggaca aattaattgg ataatttaaa aggaggaatt atgcttgaat
    39481 ttttgaatag atacccagtt ttactggaag ataaaagtgt aaaagagact aaagcgattt
    39541 tagcatttac gtctagcacg attaaagcaa attttgaagt gacgctacca gcagaagaaa
    39601 atgataaaaa atttgctgaa actttaaaaa cgtgtgaaaa gcttatcttt gagcaacttt
    39661 acaaagacaa agcagaagca gaacaatttg aaaaaattaa tgacgcaatt gctaagtcaa
    39721 aggcgcaatc agataaagcg gaaaatatga ttaaactgat gtcagcaact gttaacgatt
    39781 tgattaagac aatggctgac ggagggaaat tgaatgatac aacgcttaac aacgctagcg
    39841 aaaatagcag tacacatatt taaaaacaaa aaaggagaaa aaacaatgat gattaattac
    39901 tttgcaatgc agattgaact agggtggatt actattgatg acgttccagc attttgtcgt
    39961 gagcgagtac gtaaactaat tgaagtttct acggttggta cagaaggaaa atgaggcaat
    40021 gaatgaacat tgacatacta caaattggcg cagcaagcgg ggcgatttta tcggtagttg
    40081 gattgtgggc gtttgttgtt aatccgttta aaacagcgat gcaaaaaaac gaagatacaa
    40141 tgagcgccct taaagacaca ataaaagaac tggcttacga actaaaagac tcacagcgtg
    40201 acagggaaaa gatacataaa atcttggata tccacgagca acgactcgga aaaacagaag
    40261 acgacatcat tgtcaacaag gaacaaataa aaacattatt taataggaga aataaatatg
    40321 attaatttaa aattacgact acaaaacaaa gtaactttga tggctatttt aggagctata
    40381 tttttgctag cacagcaagt aggtattaaa ctaccatcaa atattgcgga tattgccaac
    40441 acagcagtaa cgcttttggt tttgcttgga gttgtgacag acccaacaac aaaagggcta
    40501 tcagatagcg aacaagcatt gaattaccac gagccaaaaa aataggaggg gacatgcgag
    40561 caatcactaa aatagcaatg gtactagcga tagcaatact gtacataccg cttgcagtga
    40621 ttgctttttt tatttatccg atttatttga tttttgaaaa ggaggagtaa atggcaacgt
    40681 atcaagaata taaaaacagg tcaaatggta acgcttatga cattgatggg tcgtttagtg
    40741 cacaatgttg ggacggttat gctgattact gtagattttt aggcgtaccg tacgcaaact
    40801 gtacaaacac aggatacgca agagacattt gggaacaacg gcacgaaaat ggcattttaa
    40861 actactttga cgaggtagag actatgcaag ctggggatgt tgctattttt atggtcgttg
    40921 acggtgtgac accttatagc catgtcgcta tttttgatag tgatgctggt ggtggctacg
    40981 gatggttttt tggtcaaaat caaggtggaa caaatggagc ttataatcta gtaaaaatac
    41041 catattcagc aacttatcct actgcgttta gaccaaaagt ttttaaaaac gctgttactg
    41101 taactgataa taccggttta aataaaggtg attactttat cgatgtatcg gcttatcagc
    41161 aagcggattt aactgctact tgtcagcagg caggcactac aaaaacgatt atcaaggtat
    41221 cagagtctat tgcgtggcta tctgacagac accaacaaca agctaacacg agtgacccga
    41281 ttggctatta tcattttggg cgttttggcg gtgatagtgc tttagcgcaa agggaagcag
    41341 acttgttttt gtctaactta ccaggcaaaa aagtatcata cttagtcatt gactacgaag
    41401 attccgcaag cgcagacaag caagctaaca caaacgcagt tattgcattt atggataaaa
    41461 tcgctagcgc tggttacaag cctgtttatt acagctataa gccatttaca cttaataatg
    41521 ttgattatca gcagattatt gctaaatacc ccaacagtat ttgggtcgca ggctatcccg
    41581 attatgaggt gcgctctgaa ccgctgtggg actattttcc gtcaatggac ggtgtgcggt
    41641 ggtggcaatt tacaagtgtc ggaattgctg gggggctaga taaaaatgtc gttttattag
    41701 ctgacgacaa tagccaagtg actgtaccaa aagcagataa accgcaagaa gcagttagct
    41761 ttaatcagcg tttagatgtt aatactaagc tagataactc aaatacgcca tactacgaag
    41821 caacccttag cacagactat tatgtagagt ctaagccaaa cgcaagtagt tctgataaag
    41881 aatttatcaa agcaggaact cgcgtaagag tctacgaaaa agtaaatgga tggtcacgta
    41941 ttaacgcttc tcagtctgac caatgggtcg aagataagta tttagctaat gccacacaag
    42001 tataaaacag gaggtaaaat tcctttagat aagacaaatg ccctcgcata cgcgcagaca
    42061 tgttctgatg gggggttttt ttgctaaaaa attgagggtg tttcatggcg gaaaattttc
    42121 aacacaaaag ttacagagaa caagttgaac ttttggaatc tagaggtatt gtctttagtg
    42181 gaagaaaagc taaaagcaaa agttgaacaa gcgtcaggca gtcttaaaga aggtgcaggg
    42241 aagctaaccg gtgataaaga gttagaagca aaaggatttg tcgaaaaaac aattgctaaa
    42301 ggtaaagaac tagcagatga tgctaaagat gctgttgaag aggcagtaga tgctgtcaaa
    42361 gaaaaactga aataaatatt aaccgctcat taaatgagcg gttat
//
LOCUS       DATROD010000008        40693 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.008, whole genome shotgun sequence.
ACCESSION   DATROD010000008 DATROD010000000
VERSION     DATROD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40693
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.008"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..631
                     /gene="tenA"
                     /locus_tag="VBC74_000432"
     CDS             <1..631
                     /gene="tenA"
                     /locus_tag="VBC74_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEO6941408.1"
                     /translation="YQDPFIQGIVKGRLDHDVICHYLQADNIYLGKFADIYALCLAKS
                     DNLRDKQFFLEQVDFTLNRELADGEGPHQALAAYTNRSYQDIIEKAVWYPSADHYIKH
                     MYFHFYENGIAGALAAMSPCPWIYHQLAKKIIEENQFLNGNPFNNWITFYANDTVEEL
                     MENYFRMMDYYAQNLSKEKQADLVDAFVKSCQHERRFFQMAINQEKWED"
     gene            633..1430
                     /gene="thiD"
                     /locus_tag="VBC74_000433"
     CDS             633..1430
                     /gene="thiD"
                     /locus_tag="VBC74_000433"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO6941409.1"
                     /translation="MTYLAPVLTIAGTDPSGGAGIMADLKTFQARRTYGMAVVTSVVA
                     QNTCGVRGVQHIETAIIDQQLACVYDDIKPKAVKTGMLAERETISLVASYLKKYPQPY
                     VLDPVMVATSGHRLIDSDAVEALKEDLLPLATIITPNLPEAEVLVGYDLSDEVSIIKA
                     GYDIQKQYSVRNVLIKGGHLDGLAKDYLFLEKEGLITLSNQRINTIHTHGTGCTFAAV
                     VAAELAKGQSILNAVSTAKSFITSAIETAPELGLGNGPVNHTSYQGD"
     gene            1432..2202
                     /gene="thiM"
                     /locus_tag="VBC74_000434"
     CDS             1432..2202
                     /gene="thiM"
                     /locus_tag="VBC74_000434"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEO6941410.1"
                     /translation="MYLEQLKEVNPLTICITNNVVKNFTANGLLALGASPAMSECIED
                     LEDLLKVADALLINIGTLTKESWQLYQEAIKIANKNQVPVVLDPVAAGASRFRLEVSL
                     DLLKNYPISLLRGNGSEIAALVGEKQASKGADGGKVADLESIAVKANQVFDVPVVVTG
                     ETDAIAVRGEVRLLQNGSPLMPLVTGTGCLLGAVLAAFIGSSDRSDDLACLTEAMTVY
                     NVAGEIAEKVAKGKGVGSFQVAFLDALSQMKSEMIMDK"
     gene            2216..2887
                     /locus_tag="VBC74_000435"
     CDS             2216..2887
                     /locus_tag="VBC74_000435"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001904425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEO6941411.1"
                     /translation="MKDTLKLYFVCGTVDCSRKNILTVVEEALQAGITLFQFREKGFT
                     ALQGKEKIAMAKQLQILCKQYQVPFIIDDDIDLVELIDADGLHIGQNDLPVDEARRRL
                     PDKIIGLSVSTMAEYQKSQLSVVDYIGIGPFNPTQSKADAKPAVGNRTTKAVREINQD
                     IPIVAIGGITSDFVHDIIESGADGIAVISAISKANHIVDATRQLRYEVEKALVNRQKH
                     SDVIK"
     gene            3014..4273
                     /locus_tag="VBC74_000436"
     CDS             3014..4273
                     /locus_tag="VBC74_000436"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO6941412.1"
                     /translation="MRKIIINGGKQLTGEVAVSGAKNSVVALIPATILADDVVVLDGV
                     PAISDVDSLVDIMETMGAKIKRYGETLEIDPRGVKDIPMPYGKINSLRASYYFYGSLL
                     GRYGQATLGLPGGCDLGPRPIDLHLKAFEAMGASVSYEGDSMRLATNGKPLQGANIYM
                     DTVSVGATINTMIAAAKANGRTVIENAAREPEIIDVATLLNNMGAHIRGAGTDVITIE
                     GVKSLHGTRHQVIPDRIEAGTYIAMAAAIGRGIKVTNVLYEHLESFIAKLDEMGVRMT
                     VEEDSIFVEEQEKLKAVSIKTSPYPGFATDLQQPLTPLLLTAEGNGSLLDTIYEKRVN
                     HVPELARMGANISTLGGKIVYSGPNQLSGAPVKATDLRAGAALVIAGLMAEGRTEITN
                     IEFILRGYSNIIEKLTSLGADIQLVEE"
     gene            4375..4929
                     /locus_tag="VBC74_000437"
     CDS             4375..4929
                     /locus_tag="VBC74_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6941413.1"
                     /translation="MDIWTNLGRFAFIETEHVNLRPVAYTDREAFWRIASKRTNLQFI
                     FPVQTSKKESDFLLVHSFMKEPLGVWAIEDKVSHKMFGVIRFENIDLSKKTAEIGYFL
                     KESSWGQGIMTECLKTLSFFAFQEFGMDKLIIVTHKENIASQKVALKAHFKQSRSFKG
                     SDRYTRRIRDYIEFQLTRGDYRYE"
     gene            4922..6205
                     /locus_tag="VBC74_000438"
     CDS             4922..6205
                     /locus_tag="VBC74_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO6941414.1"
                     /translation="MSKHQEILEYLENLAVGKRVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETRPRSGTVRVAQKAKVNIEKLTYAEIARISDSQVVAGTEGLSKEFSKFSIGA
                     MTHRNIEKYLVQGGLLIVGDRDEIQHLALQHQNAILVTGGFNVSPSVCRLADKLQIPV
                     MVTHYDTFTVSTMINHTLSNAKIRTDLKTVEQVYQSQMDYGFLAQDDTVKEFNLLVKQ
                     TKNVRFPIVNQANVVVGVVSVQDILGKDKEVKLATVMSKNIIVAKPRMSLANISQKMI
                     FEDLNMMPVVSDDFELLGVITRRQAVENLSMSQGTALYTYSDQILSNLQIEDGHFSFL
                     VEPAMIDHTGSLTQGVLTEFLKEICIRVLTRKHQRSIVVKQMTLYFLQPVQIDEIIMV
                     TPTIISEKRREATLDLELKLENKIIAKAMIAVKIN"
     gene            6228..7088
                     /locus_tag="VBC74_000439"
     CDS             6228..7088
                     /locus_tag="VBC74_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEO6941415.1"
                     /translation="MITLKSAREIEAMDRAGDFLASIHIGLRDIIKPGVDMWEVEEYV
                     RRRCKEENVLPLQIGVDGAVMDYPYATCCGLNDEVAHAFPRHYTLKQGDLLKVDMVLS
                     EPLDKSIVDVSSLNFDNVAQMKKYTETYSGGLADSCWAYAVGEVSQEVKDLMSVTREA
                     MYIGIEKAVIGNRIGDIGAAIQDYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLREGMVLTIEPMINTGTWEIDTDMKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            7090..8010
                     /locus_tag="VBC74_000440"
     CDS             7090..8010
                     /locus_tag="VBC74_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEO6941416.1"
                     /translation="MKLKKFFEDLLAKLEYRPIQVFMRHFQSAEMDLSAIAVAYYLLV
                     TAFPLLVIAANIFPYFHINVSDLLSLMQKNLPKNIYEPASRLAVDAFSKPSTGILGFA
                     SLTAFWTMSKSLTSLQKAINKAYGVDQHRDFVISRLVGVGTGLIILFLLTFVLIFSTF
                     SKPVLQIIVNMYDLGDTLTAWLLNLAQPVTFLTIFLGIGILYFILPNARIRKVRYVIP
                     GTLFSTFVIGFFSNLISQYVLNRVEKMVDIKTFGSVVIFILMLWFIFLAHIMILGAIL
                     NASVQEIATGKIESRRGDIMSLIQKSKEEK"
     gene            complement(8027..8482)
                     /locus_tag="VBC74_000441"
     CDS             complement(8027..8482)
                     /locus_tag="VBC74_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEO6941417.1"
                     /translation="MVKLKSLLKKSIQNEVSLYLLFGLLTSLLYLVIRQGIFNFSQDA
                     PFSAIVANIIAILFAFFTNDRFVFKQTKIGQFQRLQTFVIARLGTLGLDLILAVIFVD
                     QFPSIIGQFVQHNLNKINTIESLVSQILIILLNYILSKFVIFKDKKRQL"
     regulatory      8553..8666
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            8664..9173
                     /locus_tag="VBC74_000442"
     CDS             8664..9173
                     /locus_tag="VBC74_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEO6941418.1"
                     /translation="MNTFTTRDYAHMAIVTAIYIVLTITPPFNAIAYGAYQFRVSEML
                     NFLAFYHRKYLFAVTLGCMISNLYSFGMIDVFVGGGSTLLFVYLGTILFKQYQKDYLF
                     NGLINKAFFFFSFFFAASMITVAVELKIVAGLPLLLTWLTTAVGELASLLVGAVLVDK
                     LSRHVDFTK"
     gene            9313..11271
                     /gene="ligA"
                     /locus_tag="VBC74_000443"
     CDS             9313..11271
                     /gene="ligA"
                     /locus_tag="VBC74_000443"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEO6941419.1"
                     /translation="MENRMNELVSLLNQYAKEYYTQDNPTVSDSQYDQLYRELVELEE
                     QHPENILPNSPTHRVGGLVLEGFEKYQHEYPLYSLQDAFSKEELIAFDKRVKAEFPTA
                     SYMAELKIDGLSVSLTYVNGILQVGATRGDGNIGENITENLKRVHDIPLHLDQSLDIT
                     VRGECYLPKESFEAINIEKRANGEQEFANPRNAAAGTLRQLNTGIVAKRKLATFLYQE
                     ASPTQKETQDDVLKELESYGFSVNHHRLISSSMEKIWDFIQTIEKDRISLPYDIDGIV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVVVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPNQIKERLAHFASRDAMNITGFGPSLVEKLFD
                     AHLIADVADIYRLSIEDLLTLDGIKEKSATKIYHAIQSSKENSAEKLLFGLGIRHVGS
                     KASRLLLEEFGNLRQLSQASQESIASIDGLGGVIAKSLHTFFEKEEVDKLLEELTSYN
                     VNFNYLGKRVSTDAQLSGLTVVLTGKLEKMTRNEAKEKLQNLGAKVTGSVSKKTDLIV
                     AGSDAGSKLTKAQDLGITIQDEDWLLNL"
     gene            11283..12302
                     /locus_tag="VBC74_000444"
     CDS             11283..12302
                     /locus_tag="VBC74_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO6941420.1"
                     /translation="MKKQKKARLIYNPTSGQEIMKKNVAEVLDILEGFGYETSAFQTT
                     PTKNSARDEAARAAQAGFDLIVAAGGDGTINEVVNGIAPLKRRPKMAIIPTGTTNDFA
                     RALKIPRGNPIEATKLIGKNQIVKMDIGQAQEDNYFINIAAAGSLTELTYSVPSQLKT
                     TFGYLAYLAKGVELLPRVRKVPVKITHDKGEFIGDASMIFVAITNSVGGFEQIAPDAK
                     LDDGKFTLILVKTANLIEIMHLIRLVLAGGKHINDKRVEYIKTSYLTIEPLSDERMMI
                     NLDGEYGGDAPITLANLKNHIRFFANTDEISDDALVLDKDELAIEAIAQKFANEVNDL
                     EGLGD"
     gene            12306..14606
                     /gene="pulA"
                     /locus_tag="VBC74_000445"
     CDS             12306..14606
                     /gene="pulA"
                     /locus_tag="VBC74_000445"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEO6941421.1"
                     /translation="MDNTVIVHYHSRKKNYFNLSLWQWRDGSEGQDAHFSRFDSFGAV
                     AILKYPLPYFLSHYYVIVKDKNWHHKTIDYRIDCTKGGARKEVWIVDGDDNLYYSRQA
                     AVSSHHYSRRQPHAYDMAINSRAFDKKWGFDGWLGFEYSETKTAFRLWAPTAERVELI
                     LYHSTDETASVSKVLSMKRGTAVNYKNHKENTHGVWFTELEGNYNYQAYTYRVYYRRR
                     TFKITRDPYSIATTANGKRSIVIAPEALTPEGFKISHGKEAKWRLENPNQAVIYEMHV
                     RDFSISETSGVKTDYQGKFKGLHQKDTVNQHGDKTTFDYVQDLGVNYIQLQPIFDHHQ
                     TFDDDGHYAYNWGYDPENYNVPEASFSSNPHEPATRILELKSAIQAYHDAGIGVIMDV
                     VYNHTFSSTDSAFQLTVPDYYYRMNHNGTFQNGSGCGNETASEKEMCRKYILDSVLYW
                     VKEYNIDGFRFDLMGLHDVETMNIIRNELNKIDPRILVYGEGWDMGAGLTPQNKAKKD
                     NAYQMPGIGFFNDDVRDAVKGAEIYGEFKKGLVSGNSTEDIVAKGILGSDELVSYIDP
                     SQVLNYVEAHDNYNLNDLLWELHPNDNEKQHIYRVEVASAMNLLMQGMAFMQLGQEFL
                     RTKCYPTGDKGQLTQADKERAMNSYNAPDQVNQVNWDNVTFHKSTINFIRKIITLKTN
                     SPYFSYSSFEEIRKHVFVESAQYHSGFISFTVEEHKKIKIIFNIKGKRLQDPFIGDII
                     ISNHEEGKAIDSLDDELSVMILDITK"
     gene            14811..16679
                     /gene="glgB"
                     /locus_tag="VBC74_000446"
     CDS             14811..16679
                     /gene="glgB"
                     /locus_tag="VBC74_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEO6941422.1"
                     /translation="MSNKDELYTFGIGENFHLQNYLGVHSENGSFCFRVWAPNAENVQ
                     VIGDFTDWRNRPLQMNKIQAGVWEANSLDAREGDLYKYLVTRKGGQVVEKIDPMAVYM
                     ERRPGTASVIKVLRNKKWEDGLWMGRRKRLGFQKRPINIYEVHAGSWKKDDFGHPMTF
                     SQLKDYLIPYLVEMNYTHVEFMPLMAHPLDMSWGYQLMGYFAFEHTYGTPEEFQDFVE
                     ACHKNNIGVLVDWVPGHFIQNDDALAYFDGTATYEYQNHDRAHNYRWGALNFDLGKNQ
                     VQSFLISSALFWIEHYHIDGIRVDAVSNMLYLDYDEGPWEANQFGDNRNLEGYHFLRK
                     LNKVIKERHPNVMMIAEESTASTPITKDLESGGLGFDFKWNMGWMNDILRFYEEDPLY
                     RQYDFNLVTFSFMYIFNENFVLAFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNLYAYQ
                     MCHPGKKLLFMGSEFGQFLEWKYNDQLEWENLNDDMNQKMQRYTKQLNQFYKDHKCLW
                     RIDDSFDGLEIIDADNKSETVLSFIRKDDKGDLLLCVFNMTPVERPNFTIGVPQAGIY
                     EEVLNTEMEEFGGVWKNHNPVTKTQVATWKDYDHTLSFTLPALGASVWRIKRRLRK"
     gene            16721..17860
                     /locus_tag="VBC74_000447"
     CDS             16721..17860
                     /locus_tag="VBC74_000447"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019785022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEO6941423.1"
                     /translation="MKNEMLALIFVGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGINNVGVITQYQPLELNTHIGNGSSWGLDGIDSGVTVLQPYSATEGNRWFQGT
                     SHAIYQNIDYIDRINPEYVLILSGDHIYKMDYDDMLQTHKDNLASLTVAVLDVPLKEA
                     SRFGIMNTDSNDRIVEFEEKPEHPKSTKASMGIYIFDWKRLRTVLIDGEKNGIDMSDF
                     GKNVIPAYLESGERVYTYNFDGYWKDVGTIESLWEANMEYIGEDNKLHSRDRSWKIYS
                     KNLIAPPNFMTEDANVKDSLVVDGCFVAGNVEHSILSTNVQVKPNAIIKDSFVMSGAT
                     IGEGAKINRAIIGEDAVIGDGVVIDGSKEVEVIGYKEVAGVPNED"
     gene            17850..18983
                     /gene="glgD"
                     /locus_tag="VBC74_000448"
                     /pseudo
     CDS             17850..18983
                     /gene="glgD"
                     /locus_tag="VBC74_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687083.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
     gene            18980..20410
                     /gene="glgA"
                     /locus_tag="VBC74_000449"
     CDS             18980..20410
                     /gene="glgA"
                     /locus_tag="VBC74_000449"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEO6941424.1"
                     /translation="MKIMFVAAEGAPFAKTGGLGDVIGALPKSLSKKGHDVAVVMPYY
                     DMVDQKFGDQIENLMYFYTDVGWRHQYVGVKRLSQDNVTFYFIDNQYYFYRGHVYGDW
                     DDGERFAYFQLAALELMEKIDFIPDVLHVHDYHTAMIPFLLKEKYHWIQAYNNIRAVF
                     TIHNIEFQGQFGPEMLGDLFGVGAERYEDGTLRWNNCLNWMKAAILYSDRVTTVSPSY
                     ANEIKTPEFGKGLDQIMRMEAGKLSGIVNGIDSDLLNPETDAFLPYHFSKSNLEGKIK
                     NKLALQENLGLPQDKNVPLIGIVSRLTDQKGFDIIASELDNMLQQDIQMVILGTGYHH
                     FEETFSYFASRYPEKLSANITFDLGLAQQIYAASDIFMMPSAFEPCGLSQMMAMRYGS
                     LPLVHEVGGLKDTVVAFNQFDGSGTGFSFNHFSGYWLMQTLKLALEVYNDYPEAWKKL
                     QWQAMSKDFSWDTACVAYEQLYQQLQ"
     gene            20742..20942
                     /locus_tag="VBC74_000450"
     CDS             20742..20942
                     /locus_tag="VBC74_000450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEO6941425.1"
                     /translation="MNLAILALGFAVMGVSIGEGILVANIAKSAARQPEMFSKLQTLM
                     FTGVAFIEGTFFVLFAFTFLVR"
     gene            20974..21690
                     /gene="atpB"
                     /locus_tag="VBC74_000451"
     CDS             20974..21690
                     /gene="atpB"
                     /locus_tag="VBC74_000451"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEO6941426.1"
                     /translation="MESTSNPTVSFLGIDFDLTILAMSLLTITIIFILVFWASRKMTI
                     KPKGKQNVLEYVYELVNNTISQNLGHYTKNYSLLMFILFSFVFIANNLGLMTSLKTHE
                     HNFWTSPTANFGVDITLSLLVAFICHIEGIRKKGIGGYLKGFLSPTPAMLPMNLLEEV
                     TNVASLALRLFGNIFSGEVVTGLLLQLAVLSPFTGPLAFALNIVWTAFSMFIGFIQAY
                     VFIILSSSYIGHKVHGDEEE"
     gene            21708..22205
                     /gene="atpF"
                     /locus_tag="VBC74_000452"
     CDS             21708..22205
                     /gene="atpF"
                     /locus_tag="VBC74_000452"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEO6941427.1"
                     /translation="MSILINSTTIGDIIIVSGSVLLLFILIKTFAWKQITGIFEAREQ
                     KIANDIDTAEQARQQAEAFATKREEELSNAKTEANQIIDNAKETGLAKGDQIISEAKT
                     EADRLKEKAHQDIAQNKAEALADVKGEVADLTVLLAEKIMVSNLDKEAQSNLIDSYIK
                     KLGDA"
     gene            22205..22741
                     /locus_tag="VBC74_000453"
     CDS             22205..22741
                     /locus_tag="VBC74_000453"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEO6941428.1"
                     /translation="MNKKTQALIEQYSKSLVEVAIEHKIVEEIQQEVAALIDIFETSE
                     LEGVLSSLAVSHDEKQHFVKTLQTSCSTYLVNFLEVIVQNEREALLYPILKSVDQELI
                     KVNGQYPIQITTAVALSPEQKERLFDIAKTKLALPNGQLVEHIDPSIVGGFVVNANNK
                     VIDASVRNQLHQFKMKLK"
     gene            22757..24262
                     /locus_tag="VBC74_000454"
     CDS             22757..24262
                     /locus_tag="VBC74_000454"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEO6941429.1"
                     /translation="MAINAQEISALIKKQIEDFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFSNGAYGMAQNLESNDVGIIILGDFSEIREGDVVKRTGKIMEVPVGEA
                     MIGRVVNPLGQPVDGLGEIETTATRPVETPAPGVMQRKSVFEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRKYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYNGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDALGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGAAQIKAMKRVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEVLKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     NDILAFEEALYDYFDAHYDNLFETIRTTKDLPEEAELDAAIQAFKDQSQFK"
     gene            24278..25159
                     /locus_tag="VBC74_000455"
     CDS             24278..25159
                     /locus_tag="VBC74_000455"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEO6941430.1"
                     /translation="MAGSLSEIKDKILSTEKTSKITSAMQMVSSAKLVKSEQAARDFQ
                     VYASKIRQITTNLLKSDLVSGSDNPMLASRPVKKTGYIVITSDKGLVGGYNSKILKAM
                     MDTITDYHTENDDYAIISIGSVGSDFFKARGMNVSFELRGLEDQPSFDQVGKIIAQAV
                     EMYKNELFDELYVCYNHHVNSLTSQVRMQQMLPIKELDAEEASEDRVITGFELEPNRE
                     VILEQLLPQYTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQA
                     AITQEITEIVAGANALE"
     gene            25233..26639
                     /gene="atpD"
                     /locus_tag="VBC74_000456"
     CDS             25233..26639
                     /gene="atpD"
                     /locus_tag="VBC74_000456"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEO6941431.1"
                     /translation="MSSGKIAQVVGPVVDVVFASGDKLPEINNALIVYKNGDKSQKVV
                     LEVALELGDGLVRTIAMESTDGLTRGLEVLDTGRAISVPVGKDTLGRVFNVLGDAIDL
                     EEPFAEDAERQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALTPEIVGDEHYEVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAETFTGQPGSYVPVEETVRGFKEILDGKHDQIPEDAF
                     RMVGGIEDVIAKAEKMNY"
     gene            26652..27065
                     /locus_tag="VBC74_000457"
     CDS             26652..27065
                     /locus_tag="VBC74_000457"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEO6941432.1"
                     /translation="MAQLTVQVVTSDGIRYDHHASLITVRTPDGEMGILPGHINLIAP
                     LIVHQMKINRSHQEGVDWVAVNGGIIEVNEDQVTIVADSAERARDIDLNRAERAKERA
                     ERALEKAQTTQNIDEMRRAEVALRRAINRISVGKK"
     gene            27130..27360
                     /locus_tag="VBC74_000458"
     CDS             27130..27360
                     /locus_tag="VBC74_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEO6941433.1"
                     /translation="MILNIVTIVSNFIFIALFYQLFVDLFDWSKMIKMSPQNISKLKV
                     FILLISIVGGYVVSHFLLEVIQLCQSIFWALQ"
     gene            27423..28694
                     /gene="murA"
                     /locus_tag="VBC74_000459"
     CDS             27423..28694
                     /gene="murA"
                     /locus_tag="VBC74_000459"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEO6941434.1"
                     /translation="MDKIIVEGGQTRLQGQVVIEGAKNAVLPLLAATILPSQGKTLLT
                     NVPILSDVFTMNNVVRGLDIQVDFNCDKKEILVDASGDILDVAPYEFVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPIDLHLKGLEAMGATITQNGGDITAQAEKLKGANI
                     YMDFPSVGATQNLMMAATLASGTTTIENAAREPEIVDLAQLLNKMGAKVKGAGTETLT
                     IIGVDALHGTEHDVVQDRIEAGTFMVAAAMTSGNVLVKDAIWEHNRPLISKLMEMGVE
                     VSEEEDGIRVKADTKKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMIETVFEN
                     RFQHLEEMRRMGLQTEILRDTAMIHGGRALQGAPVMSTDLRASAALILAGMVAQGQTV
                     VGQLTHLDRGYYQFHEKLAALGANIKRVSEA"
     gene            28696..28887
                     /locus_tag="VBC74_000460"
     CDS             28696..28887
                     /locus_tag="VBC74_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEO6941435.1"
                     /translation="MASSGWKYVLKQIGLIVLVILLALLFLAVGLMLGYSVFGDGEHA
                     YSILSLDKWQNIIGKFLGK"
     gene            28962..29819
                     /locus_tag="VBC74_000461"
     CDS             28962..29819
                     /locus_tag="VBC74_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6941436.1"
                     /translation="MTKKQKQITASIVTLLFSLLAIGLGESKSLPNDHILKQVTDLII
                     SNESGKNTSLKNISGTPSRELSESVLTSNVKKQLGTNIAWNQSGAFIINQNKTDLNAK
                     VSSAPYAINEIKKVNNQIVPTKANALLTKATRQYRNREETGNGRTYWKPAGWHQINGL
                     KGSYNHAVDRGHLIGYALVGSLRGFDASTSNPKNIATQAAWANQANSNQSTGQNYYET
                     LVRKALDRHKTVRYRVTLIYDRDNLLSSGSHIEAKSSDGSLEFNVFIPNVQSGLLFDY
                     ATGKVKQTK"
     gene            30110..31150
                     /gene="pheS"
                     /locus_tag="VBC74_000462"
     CDS             30110..31150
                     /gene="pheS"
                     /locus_tag="VBC74_000462"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEO6941437.1"
                     /translation="MDLQKQLEELKISTQEKLKEMTGNHTKELQDLRVQVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDILTKAFEEQAKVVEAAKIQAQLESESVDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQVVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTMDQHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQI
                     EGLVVGENISMGDLKGTLQLISQKMFGAERKIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKQTGWIEILGAGMVHPSVLEMSGIDSEKYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDVRFTDQF"
     gene            31233..31753
                     /locus_tag="VBC74_000463"
                     /pseudo
     CDS             31233..31753
                     /locus_tag="VBC74_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            31807..34212
                     /locus_tag="VBC74_000464"
     CDS             31807..34212
                     /locus_tag="VBC74_000464"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEO6941438.1"
                     /translation="MLVSYKWLKELVDVDVTTAELAEKMSTTGIEVEGVETPAEGLSK
                     LVVGHIVSCEDVPDTHLHLCQVDTGGDELRQVVCGAPNVKTGINVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSESIIPKEFSDGIQILPEGAIPGDSIFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYGKKVHFEEKNLIEEAERAADKISVVIESDK
                     VLSYSARIVKNVTVAPSPQWLQNKLMNAGIRPINNVVDVTNYVLLTYGQPMHAFDFDK
                     FDGTTIVARNAENGEKLITLDGEERDLIADDLVIAVNDQPVALAGVMGGQSTEIDSSS
                     KTVVLEAAVFNGTSIRKTSGRLNLRSESSSRFEKGINYDTVSEAMDFAAAMLQELAGS
                     QVLSGQVTEGVLPTEPVEVSTTLGYVNTRLGTELTYTDIEEVFEKLGFAISGSEVKFT
                     VLVPRRRWDIAIQADLVEEIARIYGYEKLPTTLPEAGATAGELTSMQRLRRRVRTVAE
                     GVGLSEIITYALTTPEKAVQFSTQATNITELMWPMTVDRSALRQNVVSGMLDTIAYNV
                     ARKNSNLAVYEIGKVFEQTGNPKEDLPTEVETFTFALTGLVEEKDFQTKAKPVDFFYA
                     KGIVEALFIKLKLDVTFVAQKGLASMHPGRTATILLDGKEIGFVGQVHPQTAKQYDIP
                     ETYVAEINLSTIESQMNQALIFEDITKYPSVSRDIALLLAESVSHHDIVLAIESSGVK
                     RLTAIKLFDVYAGNNIAEGYKSMAYSLTFQNPNDNLTDEEVAKYMEKITKSLVEKVNA
                     EIR"
     gene            complement(34281..35183)
                     /locus_tag="VBC74_000465"
     CDS             complement(34281..35183)
                     /locus_tag="VBC74_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEO6941439.1"
                     /translation="MKIDDLRKSDNVEDRRSSSGGSFSSGGSGLPILQLLLMRGSWKT
                     KLVVLIILLLLGGGGLTSIFNDSSSPSSYQSQNVSRSVDNSATREQIDFVNKVLGSTE
                     DFWSQEFQTQGFGNYKEPKLVLYTNSIQTGCGIGESASEPFYCSADKKIYLDISFYNE
                     LSHKYGATGDFAMAYVIAHEVGHHIQTELGIMDKYNRMRHGLTKKEANALNVRLELQA
                     DYYAGVWAHYIRGKNLLEQGDFEEAMNAAHAVGDDTLQKETYGKLVPDSFTHGTAQQR
                     QRWFNKGFQYGDIQHGDTFSAEHL"
     gene            35361..38594
                     /gene="rexB"
                     /locus_tag="VBC74_000466"
     CDS             35361..38594
                     /gene="rexB"
                     /locus_tag="VBC74_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEO6941440.1"
                     /translation="MKLLYTDINHDMTEILVNQAAHAAEAGWRIFYIAPNSLSFEKER
                     AVLETLPQEASFAITITRFAQLARYFTLNQPNQKESLNDIGLAMIFYRALASFEDGQL
                     KVFGRLKQDASFISQLVDLYKELQTANLSILDLKYLHSPEKFEDLLAIFLVVSDLLRE
                     GEYDNQSKIAFFTEQVRSGQLDVDLKNTILIVDGFTRFSAEEEALIKSLSSRCQEIII
                     GAYASQKAYKTNFTNGNIYSAGVDFLRYLATTFQTKPEFILSKWESKSGFEMISKNIE
                     GKHDFTNSSHILDDTAKDCITIWECINQKDEVEHVARAIRQKLYQGYRYKDILVLLGD
                     VDSYKLQLSKIFEQYDIPYYFGKAETMAAHPLVHFMDSLSRIKRYRFRAEDVLNLFKT
                     GIYGEIGQDDLDYFEAYISYADIKGSKKFFTDFVVGAKKFDLGRLNTIRQSLLTPLES
                     FVKTKKQDGIKTLNQFMFFLTQVGLSDNLSRLVGQMSENEQEKHQEVWKTFTDILEQF
                     QTIFGQEKLNLDEFLSLLNSGMMQAEYRMVPATVDVVTVKSYDLVEPHSNQFVYALGM
                     TQSHFPKIAQNKSLISDIERQLINDANDTDGHFDIMTRENLKKNHFAALSLFNAAKQA
                     LVLTIPQLLNESEDQMSPYLVELRDIGVPFNHKGRQSLKEEADNIGNYKALLSRVVDL
                     YRSAIDKEMTKEEQTFWSVAVRYLRRQLTSKGIEIPIITDSLDTVTVSSDVMTRRFPE
                     DHPLKLSSSALTTFYNNQYKYFLQYVLGLEEQDSIHPDMRHHGTYLHRVFEILMKNQG
                     IESFEEKLNSAINKTNQEDVFKSLYSEDAESRYSLEILEDIARATATILRQDSQMTVE
                     SEEERFELMIDNTIKINGIIDRIDRLSDGSLGVVDYKSSAQKFDIQKFYNGLSPQLVT
                     YIDAISRDKEVEQKPPIFGAMYLHMQEPRQDLSKIKNLDDLVTKNHQALTYKGLFSEA
                     EKEFLANGKYHLKDSLYSEAEIAILQAHNQSLYKKASETIKSGKFLINPYTEDAKTVD
                     GDQFKSITGFEADRHMARARALYKLPAKEKRQGFLTLMQQEEENDDL"
     gene            38584..>40693
                     /locus_tag="VBC74_000467"
     CDS             38584..>40693
                     /locus_tag="VBC74_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEO6941441.1"
                     /translation="MTFKPFLNPEDIAVIQTEEKNSDKKQKRTPEQIEAIYTFGNNVL
                     VSASAGSGKTFVMVERILDKLLRGVPIESLFISTFTVKAAGELKERLEKKINESLKSA
                     ESDDLKQFLTQQLVGIQTADIGTMDAFTQKIVNQYGYTLGISPIFRILQDKNEQDVIK
                     NEVYADLFSDYMTGKNAASFIKLVKNFSGNRKDSKAFREMVYKVYAFSQSIDNPKRWM
                     QTVFLKGAQTYTDFEAIPDQEVSSLLNVMQTTANQLRDLTDQEDYKQLTAKGVPTANY
                     KKHLKIIENLVHWSQDFNLLYGKKGLANLARDITNVIPSGNDVTVAGVKYPIFKQLHN
                     RIVGLKHLEVIFKYQGESLFLLELLQSFVLDFSEQYLQEKIQENAFEFSDIAHFAIQI
                     LEENHDIRQIYQDKYHEVMVDEYQDNNHTQERILELLSNGHNRFMVGDIKQSIYRFRQ
                     ADPQIFNDKYKAYQDNPSQGKLIILKENFRSQSEVLDSTNSVFTHLMDEEVGDILYDE
                     SHQLKAGSPRQQERHPNNKTQVLLLDTDEDDIDDSDSQQYDISPAEAKLVAKEIIRLH
                     KEENVPFQDITLLVSSRTRNDGILQTFDRYGIPLVTDGGEQNYLKSVEVMVMLDTLRS
                     IDNPLNDYALVALLRSPMFGFNEDDLTRIAIHDVKMAFYHKVKLSYHKEGHHSDLITP
                     ELSSKIDYFMKTFQTWRDFAK"
BASE COUNT        13270 a         6300 c         8305 g        12818 t
ORIGIN      
        1 ctatcaagat ccatttattc aaggaattgt aaaagggcgt ttagaccatg acgtaatctg
       61 ccattattta caggcagata atatctacct tgggaaattt gctgatattt atgccctgtg
      121 cttggcaaaa tctgataatt tacgagataa acaatttttc cttgaacaag ttgactttac
      181 attaaatcgg gagttagcag atggtgaagg tccacatcaa gccttggcag cttatactaa
      241 tcgatcttat caggatatta tcgaaaaggc tgtatggtac ccgtcagcag atcattacat
      301 taaacatatg tattttcatt tttacgaaaa tggtattgct ggtgctttag ctgcaatgag
      361 cccatgtcca tggatttacc atcaattggc taaaaaaata attgaggaga accagttttt
      421 gaatggtaac ccttttaata attggataac tttttatgct aacgatacag ttgaagagtt
      481 aatggaaaac tattttcgta tgatggatta ttatgcgcag aatttgtcaa aagagaagca
      541 agctgatttg gtagatgctt ttgtgaaatc ttgtcagcat gagagacgtt tttttcaaat
      601 ggcaataaat caagaaaaat gggaggatta atatgactta tttagcacca gttttaacta
      661 ttgcaggaac tgatcctagc ggtggagcag ggattatggc agatttaaag acatttcaag
      721 ctcgtcggac ttacgggatg gctgttgtga ctagtgttgt tgctcagaac acttgtgggg
      781 ttagaggcgt tcagcatata gaaacagcta tcattgatca acaactagct tgtgtttatg
      841 atgatattaa gccaaaggcg gtcaaaacag gaatgttagc ggagagggaa acgattagtt
      901 tagtcgcttc atatttaaaa aaatatccgc aaccttatgt attagatcct gttatggtgg
      961 caactagcgg acatcgttta attgattcag atgccgtaga agcattaaaa gaagacttat
     1021 tgccattagc aactattatc acaccaaatt taccagaagc tgaggtttta gtgggatatg
     1081 acttatcaga tgaagtaagt attattaaag caggttacga tattcaaaaa caatattctg
     1141 ttagaaatgt tcttataaag ggaggacatc tagatggttt ggctaaagat tatttatttt
     1201 tggaaaaaga agggttgata actcttagta atcaacgtat caatactatt catactcatg
     1261 ggactgggtg tacatttgca gcagtcgttg cagcggagtt agctaaaggg caatcaattc
     1321 taaacgcggt aagtaccgct aaatcattta ttacttcagc tatcgaaaca gcgcctgagt
     1381 tgggtcttgg aaacggtcct gtcaatcata ctagctatca aggagactaa aatgtattta
     1441 gaacaactaa aagaggtaaa tcctttaacc atttgtatca ctaataatgt ggtcaaaaac
     1501 tttacagcca atggtttatt agctttagga gcttctccgg ctatgagcga gtgcatagag
     1561 gacttagaag acttgttaaa agttgcagat gcattattaa ttaatatagg aacactaaca
     1621 aaagaaagtt ggcaacttta ccaagaagca atcaaaatag caaataaaaa ccaagttcca
     1681 gttgtattag atcctgtagc ggcaggagca agtcgttttc gattagaagt gtcacttgat
     1741 ttattgaaga attatcctat tagtttactt agaggaaatg gtagtgaaat cgctgctttg
     1801 gttggtgaaa aacaagcgtc taaaggggca gatgggggaa aagtggctga tttagaatca
     1861 attgctgtaa aagccaatca agtttttgat gtgccagtgg ttgtcacagg agaaactgat
     1921 gctatcgctg ttagaggaga agttcgctta ttacaaaatg gtagtccttt aatgccactt
     1981 gttacaggaa ctggatgtct attaggggca gtattggctg cttttattgg tagttctgac
     2041 agaagcgatg atcttgcttg tctgacagag gccatgactg tttataatgt tgctggagaa
     2101 attgcagaga aagtggcaaa aggtaaggga gtgggaagtt tccaagttgc atttttagat
     2161 gccctttctc agatgaaatc agaaatgatt atggacaaat aggcggtgtc agtagatgaa
     2221 agatacattg aaattatatt ttgtttgtgg gactgtggat tgctctagga agaatatatt
     2281 gactgttgtt gaagaagcct tacaagcagg tataactcta tttcaattta gagaaaaagg
     2341 ttttacagca ttacaaggta aggaaaaaat tgctatggct aagcaactcc aaatactttg
     2401 taagcaatat caagttcctt ttattattga tgatgacatt gacttagttg agttaattga
     2461 tgctgatggt cttcatattg gacaaaatga tttacctgtt gatgaggcac ggagacgttt
     2521 accagataaa atcatcggtt tatctgtgag tacaatggct gaatatcaga aatcacaatt
     2581 atctgttgtt gattacatcg gaattggtcc ttttaatccg acacaatcaa aagcagatgc
     2641 taagcctgct gttggcaata ggacaacaaa ggcagttaga gaaattaatc aagatattcc
     2701 aatcgttgca ataggcggaa tcactagtga ttttgttcat gatattatag aatcaggcgc
     2761 agatgggatt gctgttatat cggctatttc aaaagcaaat catattgtag atgcaaccag
     2821 acaattgcgc tatgaagttg aaaaagctct ggttaatagg caaaaacaca gtgatgtaat
     2881 caaataaggc tacatcgtta attataagaa tttttaagat attatatcat cacacaatac
     2941 tggtatttga aaaagaattt aaactatgat aatatagtag agttgaattc tagttaaaga
     3001 aagatttgaa agtatgcgta aaattattat taacggtggc aagcaactga cgggagaagt
     3061 tgctgtatcg ggtgccaaaa atagtgtagt tgccttgatt cctgccacta ttcttgctga
     3121 tgatgttgtg gtcttagatg gagttccggc tatttctgat gttgacagtt tagttgacat
     3181 tatggaaact atgggagcta agattaagcg ttatggtgag acgcttgaaa ttgatccacg
     3241 tggcgttaaa gatattccaa tgccttatgg caaaatcaat agtcttcgtg catcctatta
     3301 tttctatgga agcttattgg ggcgttatgg tcaagcaaca ttaggtttac ctggtggttg
     3361 tgatttaggc ccccgtccga ttgatttaca tttaaaagct tttgaagcga tgggagctag
     3421 tgtttcttat gaaggtgatt caatgcgtct agcaacaaac ggaaagcctt tgcaaggagc
     3481 aaatatttat atggatactg tcagtgttgg tgcgacaatt aacactatga tcgcagcagc
     3541 taaggcaaat ggtcgtacgg ttattgaaaa tgctgcgcgt gaaccagaaa ttattgacgt
     3601 ggctactctt ttaaataata tgggagctca tattcgtggt gctgggactg atgttattac
     3661 aattgaaggt gtgaaatcgc tccatggcac gcgtcatcaa gtaattccag atcgtattga
     3721 agcaggaact tatattgcta tggcagcagc tatcggacga ggaataaaag tgaccaatgt
     3781 gttatatgaa caccttgaga gtttcattgc taagttagat gagatgggag ttcgaatgac
     3841 agttgaagag gattcaattt ttgtcgaaga acaagaaaag ttaaaagctg tatccataaa
     3901 aacctcacct tacccaggat ttgctacaga tttacaacaa cctctaaccc ctttattact
     3961 aacagcagaa ggaaacggga gtttattaga tactatctat gagaaacgtg ttaatcatgt
     4021 tcccgaatta gcacgtatgg gagctaatat ttctacccta gggggcaaaa tagtttatag
     4081 tggtcctaat caattatctg gtgcacctgt aaaagcgaca gatttgcgtg caggcgctgc
     4141 tttagttatc gctggcttaa tggcagaggg acgaactgaa attacgaata tcgagtttat
     4201 tttacgtgga tattctaata tcatcgagaa actaacatca ttgggtgcgg atatccaatt
     4261 ggttgaggaa tagtatatat aacaaagagt tgagaatatt tcttagctct tttcttcttg
     4321 ttttagatta agtcacttat aggtatttga aatctcttgt ggtataatat tatcatggat
     4381 atatggacaa atttaggacg gtttgcattc attgaaacag aacatgttaa cctacgtcct
     4441 gttgcctaca ctgataggga agctttttgg cgaatagctt caaaaaggac aaatttacaa
     4501 tttatttttc ctgttcaaac cagtaagaag gaaagtgatt ttcttttagt tcattctttt
     4561 atgaaagagc cgttgggagt ttgggcgatt gaagataaag ttagtcataa aatgtttgga
     4621 gttatccgtt ttgaaaacat tgatttatct aaaaagacag ctgaaattgg ctattttttg
     4681 aaagaaagtt cttggggtca aggcattatg actgaatgtc taaagacact ttcttttttt
     4741 gcttttcaag agtttgggat ggacaaattg atcatagtaa cacataaaga gaatattgct
     4801 agtcagaagg ttgctttgaa ggcgcatttc aaacaatcta ggagttttaa aggaagcgac
     4861 cgttatacac gccgtataag agactatatt gagtttcaat taactagggg tgattatcgt
     4921 tatgagtaaa catcaagaaa ttttggagta cctagaaaat ttagctgttg gtaagagggt
     4981 tagtgtacgc agtatttcaa atcatttaaa ggtgagtgat ggcactgcat accgcgctat
     5041 aaaagaggca gaaaataggg gtattgttga gactagaccg cgtagtggga cagtacgtgt
     5101 tgctcaaaaa gcaaaggtta atattgaaaa actgacttat gctgaaattg caaggattag
     5161 tgactcacaa gttgttgcag gaacagaggg gctcagcaag gagtttagta aattttctat
     5221 aggtgcaatg actcacagaa atattgaaaa atatttggtg caaggagggt tattgattgt
     5281 tggcgaccgt gatgaaatac aacatttagc cttacaacat caaaacgcta tcttagttac
     5341 aggtggcttt aatgtttccc catctgtttg tcgtttagca gataaacttc aaattcccgt
     5401 aatggtaaca cactatgata cattcacagt atcaacgatg attaatcaca ctttgtcaaa
     5461 tgccaaaatt cgtactgatt taaagaccgt tgaacaagtc tatcagtcgc aaatggatta
     5521 cggtttctta gcgcaggatg ataccgtaaa agaatttaat cttcttgtta aacaaactaa
     5581 aaatgttcgt tttccaatag tcaaccaagc taatgttgtc gttggtgttg ttagtgtgca
     5641 agatattctt ggaaaagata aagaagtaaa attggcaaca gtcatgtcaa aaaatatcat
     5701 tgttgctaaa cctcgaatga gtctagctaa tatcagtcaa aaaatgattt ttgaagattt
     5761 gaatatgatg ccagtagtat cggatgactt tgaattatta ggcgttatta caagacgaca
     5821 agctgtagaa aatttatcga tgtcgcaagg gacagcttta tacacctata gtgatcaaat
     5881 cttgtctaat ttacaaattg aagatggtca cttttcgttt ttagtagaac ccgctatgat
     5941 tgaccataca ggtagcttaa cacaaggagt tttgacggag ttcttaaaag aaatttgtat
     6001 tcgtgtctta actcgtaagc atcaacgcag tattgttgtt aaacaaatga cactttattt
     6061 cttgcaacct gttcaaattg atgaaataat aatggtaacg ccaacaatta tttcagaaaa
     6121 acggcgcgaa gctacactcg atttagaatt aaaattagaa aataaaatta ttgctaaagc
     6181 tatgatagcg gtaaaaatta attagatata aggagaataa atagaaaatg attacattaa
     6241 agtcagcacg cgaaattgaa gcaatggata gagcaggtga ttttttagcc agcatccaca
     6301 ttggacttcg tgatattatc aaaccaggag ttgatatgtg ggaagttgaa gaatacgttc
     6361 gtagacgttg taaagaagaa aacgtattgc cattgcaaat cggggttgat ggtgcagtta
     6421 tggattaccc atatgctact tgttgtgggc ttaatgatga agtggctcat gcctttcctc
     6481 gtcattatac cctaaaacaa ggtgaccttt taaaagtaga tatggtttta agcgaacctt
     6541 tagataaatc aattgttgat gtttcatcgt taaactttga caacgtggct caaatgaaaa
     6601 agtacacgga aacatatagt ggtggtttgg cagactcatg ttgggcttat gctgtaggtg
     6661 aagtttcaca agaggtgaaa gacttaatgt ctgtgacaag agaagcaatg tatattggga
     6721 ttgaaaaagc agttatcgga aaccgtattg gtgatattgg agctgctatt caggattatg
     6781 cagagagtcg tggttatgga gttgttcgtg atttggttgg tcacggtgtt ggtccaacaa
     6841 tgcatgaaga gcctatggtg cctaactatg gtacagcagg tcgaggtctc cgtcttcgtg
     6901 aggggatggt tttaaccatc gaaccaatga ttaatactgg tacttgggaa attgatacag
     6961 acatgaaaac tggttgggca cataaaacct tagatggtgg tctttcttgt caatatgaac
     7021 atcaatttgt tatcactaaa gatggtcctg ttattttaac gagtcaagga gaagaaagaa
     7081 cttactaaaa tgaaattaaa aaaattcttt gaggatttgc tggctaaact agaatataga
     7141 cccattcaag tttttatgcg tcattttcaa agtgcagaga tggacttatc agccatagca
     7201 gttgcttatt atctattagt gacagctttt ccattactag ttattgctgc taatattttt
     7261 ccttattttc atattaatgt gtcggattta ttgtcactta tgcaaaaaaa tcttccgaaa
     7321 aatatttatg aacctgcctc acgcttagca gtagatgctt tttcgaaacc gtctaccggc
     7381 attttgggat tcgcgtcatt aacagctttt tggacaatgt ctaaaagttt gacctctctt
     7441 caaaaagcca ttaataaagc atacggtgtt gaccaacaca gagactttgt gattagtcgt
     7501 ttagtgggtg tggggactgg gctgatcatc ctatttttac tgacttttgt tttaattttt
     7561 tcaacctttt caaaaccagt tttacagata attgtaaaca tgtatgattt gggagatact
     7621 ctaacggcgt ggcttttaaa tcttgcacaa ccagttacat ttttgactat atttttaggt
     7681 attggaattt tatactttat tttacctaac gcacgaatta gaaaagtacg ttatgtcatt
     7741 ccagggacat tattttcaac gtttgttatt ggttttttta gtaatttaat tagccaatac
     7801 gtcttaaata gggttgaaaa aatggtagat attaaaacgt ttggttcggt cgttatcttt
     7861 atcttaatgc tatggtttat tttcttagcc cacattatga ttttaggtgc aatactgaat
     7921 gccagcgttc aagaaattgc gacaggaaaa attgaatcaa ggcgtggaga tattatgtct
     7981 cttatccaaa aatccaaaga agaaaaataa gaaaagacag ctcttgttag agttgccttt
     8041 ttttgtcttt aaaaataaca aatttgctca agatgtagtt gagtaaaata attaatattt
     8101 gagatacaag gctttcaatg gtgttgattt tatttaaatt gtgctgaacg aattgtccga
     8161 taatagaagg aaattgatca acaaaaataa cagctaatat caaatctaat cctaatgtgc
     8221 ctaatctagc gatgacaaat gtttgtaagc gttgaaactg tccaatttta gtttgtttaa
     8281 aaacaaatcg atcatttgta aaaaaagcaa aaagtatagc tattatgtta gcaactattg
     8341 cagagaatgg agcgtcctgc gagaaattaa aaatcccttg tcgaataact aaataaagaa
     8401 gggatgttaa tagtccgaac agtaaatata agctgacttc attttgaata ctttttttca
     8461 gtaacgattt taatttaacc atatttttat tatatcacgg atgcttcaaa gtgatgagga
     8521 aatctcccat aagcttggct ttactttttg tttgtgatat acttagtaag ttgttttaca
     8581 caacccttga tgcttagttc cctttcatca agcatattac aaacgttaga agtgttaact
     8641 gtctaaacgt taaaggagaa attatgaaca catttacaac tcgcgattac gctcacatgg
     8701 ctattgtaac agctatctat atcgttttaa ccataacgcc accttttaat gctattgctt
     8761 atggtgctta tcaattccgt gtttcagaaa tgctaaattt cttggccttt tatcaccgaa
     8821 aatacctttt tgcggtaacg ttaggatgta tgatttctaa tctttatagt tttggaatga
     8881 ttgatgtctt tgtaggtggt ggttcaactt tactttttgt ttatcttgga accattttat
     8941 tcaagcaata tcaaaaagat tatctattta atggccttat taacaaagca ttctttttct
     9001 tttctttctt ctttgctgct tcaatgatta cggtagcagt tgaattgaaa attgtagcag
     9061 gattaccttt attattaaca tggttaacga cagctgttgg tgaattggct tcacttttgg
     9121 ttggagcagt ccttgtggat aagttgagta gacacgtcga ttttacaaaa taaatatatt
     9181 ataaagcgcg agacattgat ctcggctttt ttgtttggtt ttatagttag catagctaga
     9241 aaatgctgca acttttatga gaaatgctcc aatttttatt aatatcctta gtctttgtga
     9301 taaaatagaa atatggaaaa tcgtatgaat gaattagtat ctttgttgaa tcagtacgca
     9361 aaagagtatt atacacaaga taatcccaca gtttctgaca gtcaatatga ccagttatat
     9421 cgagagttag tagaattaga agagcaacat ccagaaaaca ttttgccaaa tagtcccaca
     9481 catcgagtgg gtggtttagt tttagaaggc tttgaaaaat accaacatga atatcctttg
     9541 tattcattgc aggatgcttt ctcaaaagaa gaattgatag cctttgataa acgtgttaaa
     9601 gcagagtttc caactgcttc ttatatggca gagctgaaaa ttgatggtct atctgtctcg
     9661 ttgacttatg taaatggtat tttacaggtt ggggcaacac gtggtgatgg caatattgga
     9721 gaaaatatta ctgaaaattt aaagcgtgtt catgatattc ctctacattt agaccaaagt
     9781 ttagatatta ctgttcgcgg agagtgctac cttcccaaag aatcttttga agctattaac
     9841 atagaaaaac gtgcaaatgg tgagcaggaa tttgctaatc ctaggaatgc agcagcagga
     9901 accttgcgcc aattaaacac agggattgtt gcgaaacgta aattagcaac ttttctttat
     9961 caagaagcta gccctacgca aaaagaaaca caagatgatg ttttaaaaga gctagaaagc
    10021 tatggctttt cagtcaatca tcatcgttta attagctctt ctatggagaa aatttgggat
    10081 tttatccaaa ctatagaaaa agatagaatt tccctccctt atgatatcga tggtattgtt
    10141 attaaggtta atagcttagc aatgcaagaa gagttgggct tcactgtaaa ggcacctcgt
    10201 tgggcgattg catataaatt ccctgctgaa gaaaaggaag cagaaatatt atctgttgat
    10261 tggacagttg ggcgaacagg agttgtaaca ccaacagcta acctaactcc agttcaacta
    10321 gcagggacaa ccgttagtag ggccactctg cataatgtag attatattgc cgaaaaagat
    10381 atccgtattg gagatactgt tgttgtatac aaagcagggg atatcatccc tgcagtctta
    10441 aatgtcgtta tgagtaagcg aaaccagcaa gaagtcatgc ttattcctaa actatgtcct
    10501 tcttgtggta gtgaattagt tcattttgaa gatgaggtgg ctttacgttg tattaatccg
    10561 ctgtgcccca accaaatcaa agaacgttta gctcattttg ctagccggga tgctatgaat
    10621 attacaggtt ttggtccatc cttagtcgaa aaattatttg atgctcattt aatagcagat
    10681 gttgcagata tttatcgttt aagtatagag gatttattaa ctttagatgg tattaaagag
    10741 aaatctgcaa ctaagattta tcatgcaatc caatcttcaa aagaaaattc tgctgaaaaa
    10801 cttctttttg gcctaggtat tcgtcatgtg ggaagcaagg cgagccgcct tttattggag
    10861 gaatttggta atttgaggca actaagtcaa gcaagtcaag aaagtattgc ctcaattgat
    10921 ggactaggag gggttattgc caaatcactt catacgttct ttgaaaaaga agaggtagat
    10981 aaactactag aggaattaac ttcctataac gttaatttta attatctagg aaaacgagtt
    11041 agtacagatg cacaactttc aggtttaaca gtcgttttaa ccggaaaact tgaaaagatg
    11101 actcgtaatg aagcaaaaga gaaattacaa aaccttggtg ctaaggtgac aggtagcgtt
    11161 tctaaaaaga ctgatttaat tgttgcagga agcgatgcag gaagtaaact aaccaaggct
    11221 caagatttag ggattacaat acaagatgaa gactggttac tcaaccttta agtgaggaag
    11281 aaatgaagaa acaaaaaaaa gcgcgtttaa tttataaccc aacttctggt caagaaatta
    11341 tgaaaaaaaa tgtagcggaa gttcttgata tacttgaagg atttgggtat gaaacatcag
    11401 cctttcaaac aacgccaact aaaaattctg ccagagatga agctgctaga gctgctcaag
    11461 ctggttttga tttgattgtt gcagcaggtg gagatggtac tattaatgaa gttgttaatg
    11521 gtattgctcc tttgaaacgt cgtcctaaga tggcaattat tccaactgga acaactaatg
    11581 attttgctcg tgccttaaaa ataccgcgag gtaatcctat tgaagcaact aaattaattg
    11641 gtaaaaatca aatcgttaag atggacattg gtcaagctca agaggacaat tattttatta
    11701 atattgcagc tgcaggtagt ttgacagagt taacttatag tgtaccaagt caattgaaaa
    11761 caacgtttgg ctatcttgct tatctagcaa aaggtgttga attattacct cgagttcgaa
    11821 aagtaccagt caaaattact cacgataagg gtgaatttat cggtgatgct tcaatgattt
    11881 ttgttgccat cacaaattct gtaggagggt ttgaacaaat tgcacctgat gccaaattgg
    11941 atgatgggaa atttaccctt attttagtaa aaactgcaaa tcttattgag attatgcatc
    12001 tcattcgttt ggtgctagca ggtggaaaac atattaatga taaacgagta gagtatatta
    12061 aaacgtctta tctaactatc gaacccctat cagacgagcg tatgatgatt aacttagatg
    12121 gagaatatgg aggagatgct cccattactc tagcaaacct taaaaatcat attaggttct
    12181 ttgctaatac tgatgaaatt tcggatgatg ctttagtttt agataaagat gaattagcga
    12241 tagaagctat tgcacagaag tttgctaatg aggtaaatga tttagaagga ttaggagatt
    12301 gatgaatgga taatactgtc attgtacatt accatagccg aaagaaaaat tactttaatt
    12361 taagtttatg gcagtggcgc gatggctcag agggtcaaga tgctcatttt tcacggtttg
    12421 acagtttcgg tgctgttgcg atactaaaat atcccttgcc atatttttta agccattatt
    12481 atgtgattgt taaggataaa aattggcatc ataaaacaat agattatcgc attgattgta
    12541 ctaaaggtgg tgcacgtaaa gaagtttgga ttgtagatgg ggatgacaat ctgtattatt
    12601 ctcgtcaagc agcagttagt agccatcatt atagtagacg ccaacctcat gcctacgata
    12661 tggcaatcaa tagtagagcg tttgataaaa aatggggatt tgatggttgg ttaggttttg
    12721 aatattcaga aactaaaacg gcttttagat tgtgggcccc tactgcggaa cgtgtagaac
    12781 tgattttata tcattcgaca gatgaaacag caagtgtatc aaaagttctt tctatgaaaa
    12841 gagggacagc cgtcaattat aaaaatcata aagaaaacac ccatggcgtc tggtttactg
    12901 aacttgaagg aaactataat taccaagcat atacttatcg agtctactat agacgacgta
    12961 cctttaaaat tacgagagac ccttattcca ttgcaacgac tgcaaatggt aaaaggtcta
    13021 ttgttattgc tccagaagca ttaactccgg aagggtttaa aataagtcat ggaaaagaag
    13081 ctaagtggcg gctcgaaaac cccaatcaag cagtcattta cgaaatgcat gtgcgtgatt
    13141 tttcgatatc tgagacttca ggagttaaga cagattatca aggaaaattt aagggattgc
    13201 atcaaaaaga tacagttaat cagcatggtg ataaaacgac ctttgattat gtgcaagact
    13261 taggagttaa ctatatccaa cttcaaccaa ttttcgatca tcatcagacg tttgatgatg
    13321 atggtcacta tgcttataat tggggatatg atcctgaaaa ctacaacgtt ccagaagcaa
    13381 gcttctctag taatccacat gaacctgcaa cgcgtatttt agaattaaaa tctgctatcc
    13441 aagcgtacca tgatgcaggc attggtgtta ttatggatgt tgtctataat catacttttt
    13501 catcaactga ttctgcattt caactaacgg ttccagatta ttattatcga atgaatcaca
    13561 atggaacatt tcaaaatggt tcaggctgtg gtaatgaaac agctagtgaa aaagaaatgt
    13621 gtcgtaagta tattcttgat tcagttttgt attgggtgaa agaatataat atcgatggtt
    13681 ttcgatttga tttgatggga ctacatgatg tagaaacgat gaacattata agaaatgagc
    13741 taaataaaat cgaccctcgt attttggttt atggagaagg atgggacatg ggagctggtc
    13801 tcacgcctca aaataaagct aaaaaagata atgcttatca gatgcctgga attggctttt
    13861 tcaatgatga tgtaagagac gctgtcaaag gtgcggaaat ttacggagaa tttaaaaaag
    13921 gcttagtttc tggaaacagt acagaagata ttgttgcaaa aggcatttta ggaagtgatg
    13981 agttagtatc atacattgat cctagtcaag ttttaaatta tgttgaagca catgacaact
    14041 acaacttaaa tgatttgcta tgggaattac atcctaatga caacgagaaa caacatattt
    14101 accgagttga agtagctagt gctatgaatc tattgatgca agggatggct ttcatgcaat
    14161 taggtcagga gtttcttcga acaaagtgtt atcctacagg tgataaaggt caacttactc
    14221 aagcagataa agaaagagct atgaattctt acaatgcccc agaccaagtt aatcaggtca
    14281 actgggataa tgttaccttt cataagagta ctattaattt tattaggaaa ataattactt
    14341 tgaagacaaa ctctccttac ttttcttact ctagctttga agagattcgc aaacatgttt
    14401 ttgtagagtc tgctcaatac cattcaggtt tcataagttt tacagtcgaa gaacataaga
    14461 aaataaaaat aattttcaat attaaaggaa aacgcttgca ggatcctttc ataggtgata
    14521 taataatatc aaatcatgaa gaaggaaaag cgatagattc attagatgat gagctttctg
    14581 taatgatcct tgatatcaca aaataacctg aaaacattca ggttattttt gtgtatatta
    14641 aggaatttga tttgaatttg agtttcttat attttagtta atttttcgga aatatagagg
    14701 gtgaaatatt ttcagatgcg tctgtatgag gaaaatgcat tcctcttttt ttgtaccatt
    14761 gaggaaatat ttttgtctta tattgactag tagacctgtg aggtgagtag atgtccaata
    14821 aagatgagtt atatactttt ggaataggtg aaaattttca tctccagaat tatttaggcg
    14881 tacatagcga aaatggaagc ttttgtttca gagtttgggc tcctaatgct gaaaatgttc
    14941 aagttattgg agattttaca gattggcgaa atcgacctct ccaaatgaac aaaattcaag
    15001 caggagtttg ggaagctaac agtttggatg cgcgcgaagg tgatttatat aaatatttgg
    15061 ttactcgtaa aggtggtcaa gtagttgaga aaatcgatcc tatggcagtt tatatggaaa
    15121 gacgtcctgg aactgcaagt gttattaaag ttcttagaaa taaaaagtgg gaagatggtc
    15181 tttggatggg aaggcgcaaa cgtctgggtt ttcagaagcg tccaattaat atttacgaag
    15241 ttcatgctgg ttcatggaaa aaagatgatt ttggacatcc tatgactttt tctcagttaa
    15301 aagattactt aatcccctac cttgttgaga tgaattatac tcatgttgaa tttatgccct
    15361 taatggcaca tccgcttgat atgagttggg gatatcaatt aatggggtat tttgcttttg
    15421 aacataccta tggtactcca gaagaatttc aagactttgt agaagcatgt cataaaaata
    15481 atataggagt tcttgtagat tgggttccag gacactttat tcagaatgac gatgctttag
    15541 cctatttcga tggaactgct acttatgagt atcaaaatca tgatcgtgcg cataactatc
    15601 gatggggagc tctgaacttt gatttaggaa aaaatcaagt acaatccttt ttgatttcga
    15661 gtgccttgtt ctggattgaa cattatcata ttgatggtat tcgtgttgat gcggttagta
    15721 atatgttata tctcgattat gatgaaggac catgggaagc taaccaattt ggggataatc
    15781 gtaatttgga aggatatcac tttttaagga aacttaacaa agtaattaaa gagcgtcatc
    15841 ctaatgttat gatgattgct gaagagtcaa cagcttctac cccaataact aaagacttag
    15901 agtctggagg attgggtttt gactttaaat ggaatatggg ctggatgaat gatatcctgc
    15961 gtttttatga agaagatcct ctctataggc aatatgattt taatttagtg acttttagtt
    16021 ttatgtatat attcaatgag aactttgtac tagctttttc acatgatgaa gtagtacatg
    16081 gtaaaaagag tatgatgcat aaaatgtggg gtgaccgtta taatcaattt gctggtttac
    16141 gtaatttata tgcatatcaa atgtgtcatc ctgggaaaaa acttctcttt atgggaagtg
    16201 agtttggtca atttttagaa tggaaatata atgatcaact agaatgggaa aacttaaatg
    16261 atgatatgaa ccaaaagatg caacgctata ctaaacaatt gaatcaattt tataaagatc
    16321 ataagtgcct atggcgtatt gatgattcct ttgacgggct agaaattatt gatgcggata
    16381 ataaatctga aactgtttta tcttttatac gaaaagatga taaaggcgat ttgttacttt
    16441 gtgtctttaa tatgactcct gtagaacgcc ctaactttac tattggtgtt ccgcaagcgg
    16501 gtatttatga agaagtttta aatactgaaa tggaagagtt tggaggtgtt tggaaaaacc
    16561 acaatcctgt aacgaaaaca caagtggcta catggaaaga ttatgatcat actttaagtt
    16621 ttacattacc agcccttggg gcaagtgttt ggagaataaa acgtcggctt cgtaaataag
    16681 tctgcaattt agtaacctca ttttgaaagg aaagaataat atgaaaaatg aaatgttagc
    16741 actcattttc gtcggaggac aaggcactcg actcggaaaa ttgactcaga gcattgcgaa
    16801 acctgctgtt caatttggag gtcgttaccg tattattgac tttgcgcttt caaattgtgc
    16861 gaattcaggt attaataatg ttggagttat tactcagtat caaccgctgg aactcaatac
    16921 tcatattgga aacggatcta gctggggact agatggtatc gattcgggag taactgtatt
    16981 acagccatat tctgcaacag aaggtaatcg ctggtttcaa ggtaccagcc acgctatcta
    17041 tcaaaatatt gactacattg atcgtattaa tccagaatat gtactcattc tctctgggga
    17101 ccacatctat aaaatggact atgatgacat gctacagacg cataaagata atttagcaag
    17161 cctaacagtc gctgttttag atgttccttt aaaagaagca agccgttttg gaattatgaa
    17221 tactgattca aacgaccgta ttgtcgaatt tgaagaaaaa cctgaacatc ctaagtcaac
    17281 aaaagcttca atgggaatat acatttttga ctggaagaga ttacggacag ttttaattga
    17341 tggtgaaaag aacggcatag acatgtcaga ctttggtaaa aatgttatac cagcatacct
    17401 tgaatcgggt gaacgtgttt atacctataa ttttgatggg tattggaaag atgttggtac
    17461 tattgaatcc ctttgggaag ctaatatgga atatattgga gaagataaca aattacatag
    17521 tcgtgatcgt tcatggaaaa tctattctaa aaatttgatt gcaccaccta atttcatgac
    17581 agaagatgca aatgttaaag attctttagt tgtagatgga tgctttgttg caggaaatgt
    17641 tgagcactcc attttatcaa ctaatgtcca ggtaaaacca aatgctatta ttaaagactc
    17701 ctttgttatg agtggcgcga ctatcggtga aggtgcaaag ataaatcgag ctattatcgg
    17761 tgaggatgct gttattggtg atggtgttgt gattgatgga agtaaagaag tagaagttat
    17821 tggttataaa gaagtagcgg gggttccaaa tgaagattga taaatatact gcgattttag
    17881 gaaatactgt tagttaccca gatatgcaca gtttgacgga taatcgtccg cttgcttctc
    17941 taccttttga tggtaagtat cgtttaattg attttcaatt atcctcttta gctaatgcag
    18001 gcgttagaag tatttatggt atttttcgtg ggaataatgt tcgctctgtt tttgaccata
    18061 ttagaagtgg tcgtgagtgg ggcttaggta cactgttgag tcactattct ttaggattct
    18121 atgatacaga cgatgatact cgtacagtca atggggatta ttacgattaa attttaactt
    18181 acttgaggcg ttcgggtagc gaccagacga tatatatgag ttgtgatatg ctttgtaata
    18241 ttgacttaag tcaagtaatc cacttgcata atgcaaatgg tgtgcaagcc accgtggttt
    18301 ataaaaaaat gccgagatca gctattgcag aggctaatca agtcttgacc attgatgaat
    18361 ctgatcatgt agtgagtcta ggtaaagctg tagataatga cgataaccaa aaaatgtctg
    18421 ctgagattta tgttatcgat acaccttggt taatcgaaaa aatggaagag gaagctcaaa
    18481 ataatgaacc acgtaaactc cgcttcttac ttcgtgattt aattgttgaa agtaatgctt
    18541 tggcttttga atacactggt tatttgtcaa acatttcttc tattaaatcc tattatgatg
    18601 ctaacatgga catgttaaca ccaaataaat tttattcgct cttcttttca aaccaaaaag
    18661 tttatactaa ggtgaaaaat gaggaagcta cttattttga taaacaatca aatgtctcta
    18721 attcgcaact agcctatgga agtattatta aaggttattt agaccattct atcgtttcta
    18781 gaaattgtct ccttgaaaaa gggactcgag tagtcaatag tattatcttt ccaaaagtaa
    18841 aaatcggaga aggggcaacc attgaaaata ctattattga caaatgcgtt aaggttgcat
    18901 ccggagttac attgaaaggt agtttagata aaccgttagt gattcctaaa ttttcagaga
    18961 ttaatgagga tattatacaa tgaaaataat gtttgttgcg gcagagggtg ctccctttgc
    19021 taaaacaggt ggtttgggag atgtgattgg cgcactccca aaatcactta gtaaaaaagg
    19081 tcatgatgtc gctgttgtca tgccatatta tgatatggta gaccaaaaat ttggagacca
    19141 aatagaaaac ctcatgtatt tctatacaga tgttggctgg cgtcatcaat atgtaggagt
    19201 taagagactt tctcaagata atgttacgtt ctactttatt gataatcaat attattttta
    19261 tagaggtcat gtctatggtg attgggatga tggggagcgt tttgcttatt tccaattagc
    19321 tgctttagag ttgatggaga aaatagattt tattcccgat gtcttacacg tccatgatta
    19381 tcatacagca atgattcctt tcttattgaa agagaagtac cactggattc aggcctataa
    19441 taatataaga gcagttttta ctattcacaa tattgaattt caagggcagt ttggtccaga
    19501 aatgttagga gatttatttg gagttggcgc tgagagatat gaagatggaa ctcttagatg
    19561 gaataattgt cttaattgga tgaaagcagc aattctttac tcagatagag tgacaacggt
    19621 atcaccaagc tacgcaaatg aaataaaaac tccagaattt ggtaaaggtt tagaccaaat
    19681 tatgcgtatg gaagcaggta aattatctgg aatcgtaaat ggtattgaca gcgatttgtt
    19741 aaatccagaa acagatgctt ttttgccata tcatttttca aaaagcaact tagagggcaa
    19801 aattaaaaat aagttggctt tacaggaaaa tttgggcttg cctcaagata aaaatgtacc
    19861 attaattggt attgtttcac gacttaccga tcaaaaagga tttgatatta ttgcttctga
    19921 attggacaat atgcttcaac aagatattca aatggttatt ttaggaacag gctatcacca
    19981 ttttgaagaa acgttctctt attttgctag tcgttaccct gaaaaactat ctgctaatat
    20041 tacttttgac ttaggattag cgcaacagat ttatgcggct tcagatatct tcatgatgcc
    20101 aagtgctttt gaaccttgtg gtctctcgca gatgatggct atgagatatg gtagcttacc
    20161 cttggtacat gaagttggag gattaaaaga tactgtggta gccttcaatc aatttgatgg
    20221 tagtggtaca ggtttttcat ttaatcattt ttcaggctat tggttaatgc aaactctaaa
    20281 actagcatta gaagtttata atgattatcc tgaagcgtgg aaaaagttac aatggcaagc
    20341 tatgtcaaaa gatttttcat gggatacagc atgtgttgct tacgaacaac tgtatcagca
    20401 actacaataa tcactttatt ttagtgatta gttgataagc ttatcgaatc tctatgctaa
    20461 acgtttctta tgtaaaacca ctttagtata gagattttat tatcttataa ggtaaaatat
    20521 tattaatgta aagagacact atatcaaaaa taaagtgttg tcatatctta ataagggatt
    20581 aaattaaagc taaaaaaact aaaaattgtt atttgaaaag gattgtgtaa accatctcaa
    20641 actatttcat gaaaacgtaa tcaaatgagg aaattcattg acatgcacaa gaaataggaa
    20701 taaaataacg gagtaaatat tttttaaagg agaattctat tatgaattta gctattttag
    20761 cacttggttt cgccgtgatg ggtgtttcaa tcggtgaagg aattttggtt gcaaatattg
    20821 ccaaatcagc ggctcgtcag cctgaaatgt ttagcaaact gcaaacattg atgtttacag
    20881 gtgtggcctt cattgaaggt accttcttcg ttctatttgc gtttacattc ttagtaagat
    20941 agacgacaat ttagaaaaaa ggagggatga ggattggaat ctacaagtaa tccaaccgta
    21001 tcttttttag gcatcgattt tgatttaacc atcctagcta tgtctctctt gacaattact
    21061 attatcttta tcttagtctt ctgggctagt cgtaagatga caataaaacc aaaaggtaag
    21121 cagaatgtct tagaatatgt ctatgagctt gttaataata caatctcaca aaatttagga
    21181 cattatacta aaaattatag tttattgatg tttattttat tcagttttgt cttcattgct
    21241 aacaacttag gattgatgac atcattgaaa acacatgagc ataatttttg gacatctccg
    21301 accgctaatt ttggtgtaga tattacatta tcattattag tagcatttat ttgtcatatt
    21361 gaaggtattc gcaaaaaagg aattggtggt tatctcaaag gtttcttatc accaacacca
    21421 gcaatgcttc cgatgaattt attggaagaa gttactaatg ttgcttcatt agcattgcgt
    21481 ctttttggta atatcttttc tggtgaagtt gtcactggat tacttttaca acttgcagtt
    21541 ttatcaccat ttacaggtcc actagctttt gcattgaata tcgtttggac agcattttca
    21601 atgtttattg gtttcatcca agcgtatgtg ttcattatct tgagctcaag ttatattgga
    21661 cacaaagttc atggtgacga agaagaataa gaaaagagag gagaaatatg tcaatactta
    21721 ttaatagtac aacgattggt gatatcatta tcgtatctgg ctctgtgctt ctcttattta
    21781 tcttaattaa gacttttgca tggaaacaaa tcacaggtat ttttgaagcc cgtgaacaaa
    21841 agatagctaa tgatattgat actgctgaac aggcacgtca acaagctgaa gcttttgcaa
    21901 ctaagcgtga ggaagaacta tctaacgcta aaactgaagc taatcaaatc attgataatg
    21961 ctaaagagac aggtcttgca aaaggtgatc aaattatttc agaagcaaaa actgaagctg
    22021 atcgattaaa agaaaaagca catcaagata ttgcccaaaa taaggcagaa gcactagcag
    22081 atgttaaggg tgaagtagca gatttaacag ttttattagc tgaaaaaatt atggtatcaa
    22141 atcttgataa agaagctcag agtaatctca ttgatagcta tattaaaaag ctaggagatg
    22201 cctaatgaat aaaaagacgc aggctctaat tgagcaatat tcaaaaagcc ttgttgaagt
    22261 tgccattgaa cataaaattg ttgaagaaat ccaacaagaa gtagcagctt taattgatat
    22321 ttttgaaaca agcgagcttg agggtgtcct gtcaagtctt gccgtttcac atgatgaaaa
    22381 acaacatttt gtgaagacat tgcaaacatc atgttcaact tatcttgtta atttccttga
    22441 ggttattgta caaaatgaac gtgaagcact tctatatcct attttgaaat cagttgatca
    22501 ggaacttatc aaagtgaatg gtcaatatcc aattcaaatt acaactgcag ttgctttgtc
    22561 tccagaacaa aaagaacgtc tttttgatat tgctaaaact aaattagctt taccaaatgg
    22621 acagttagtt gagcacattg acccatctat tgttggtgga tttgttgtca atgcaaacaa
    22681 taaagtaatt gatgcaagtg ttcgtaatca attacatcaa tttaaaatga aattaaaata
    22741 gaaagtggtg tgacttttgg caattaatgc acaagaaatt agcgctttaa ttaaaaagca
    22801 aattgaagac ttccagccaa attttgacgt cacagaaact ggtattgtta catatatcgg
    22861 tgatggtatt gctcgtgctc gtgggctaga taatgctatg agtggagaac ttcttgaatt
    22921 ttcaaatggt gcgtacggta tggctcaaaa ccttgagtct aatgacgtgg gtatcattat
    22981 tttgggtgat ttctcagaaa ttcgtgaagg tgatgtggta aaacgtactg gtaaaattat
    23041 ggaagtacca gtaggggaag ctatgattgg acgtgtcgtc aatccgcttg gtcaaccagt
    23101 tgatggacta ggtgaaattg agacgacagc aacacgccca gttgaaacac cagcacctgg
    23161 tgttatgcaa cgtaaatcag tttttgagcc tcttcaaaca ggtctcaaag cgattgatgc
    23221 tttggttcct atcggacgtg ggcaacgcga attgattatc ggtgaccgcc aaactggtaa
    23281 gacgtctgta gcgattgatg caatcttgaa tcaaaaaggt caagatatga tctgtatcta
    23341 tgtggctatc ggtcaaaaag agtctacagt acgtacacaa gttgaaactt tgcgtaaata
    23401 tggtgccctt gattacacaa tcgttgtgac agcttcagca tcacaaccat ctccattact
    23461 tttcattgct ccatatgcag gagtagcaat ggctgaggaa ttcatgtata acgggaaaca
    23521 cgttttgatt gtttatgatg atttatcaaa acaagccgta gcttatcgtg aactttcgct
    23581 tcttcttcgc cgtcctccag gtcgcgaagc ttacccaggg gatgttttct atcttcatag
    23641 tcgtcttttg gaacgttcag ctaaagtatc agatgcttta ggtggaggtt cgattacagc
    23701 gcttccgttc attgaaacac aagcaggtga tatctcagca tatattgcta caaacgttat
    23761 ttcaattact gacggccaaa ttttcttgca agaaaatctc tttaactcag gtattcgtcc
    23821 ggcgattgat gcaggttcat cagtatctcg tgttggtggt gcagcgcaaa ttaaagcgat
    23881 gaagagagtt gcaggtacgc ttcgtctaga tcttgcttct tatcgcgaac ttgaggcatt
    23941 cactcaattt ggttcagacc ttgatgcagc aacacaagca aaactaaatc gtggtcgtcg
    24001 cacagtggaa gtgttaaaac aaccattaca caaaccattg ccagttgaga aacaagttgt
    24061 gattttatat gctctaacac atggattcct tgatgatgta cctgtaaatg atattttagc
    24121 ttttgaagaa gctttgtatg attactttga tgctcattat gataatctct tcgaaacaat
    24181 ccgcacaaca aaagatttac cagaagaagc agaattagat gctgctattc aagcatttaa
    24241 agatcaatca caatttaaat aataggaggg ttctaatatg gcaggctctc ttagtgaaat
    24301 taaagacaag attctttcta cagaaaaaac aagtaagatt actagtgcga tgcagatggt
    24361 ctcatctgcc aagttggtta aatctgaaca agctgctaga gattttcaag tgtatgcctc
    24421 taaaattcgt caaattacta ctaacctctt gaagtcagac ttagtatctg gatcagataa
    24481 ccctatgtta gcttcacgtc cggtgaagaa gacaggttat attgttatta cttctgataa
    24541 gggtttagtt ggtggttaca attcaaaaat cttaaaagcg atgatggaca ctataactga
    24601 ctatcatact gagaatgatg attatgccat tatttcaatt ggtagtgttg gttctgattt
    24661 tttcaaagca cgtggaatga atgtttcttt cgaattacgt ggtttagagg accaaccaag
    24721 ttttgatcaa gttggtaaaa ttattgctca agcagttgaa atgtacaaaa atgagttgtt
    24781 cgatgaatta tatgtttgct acaatcacca tgttaacagt ttaactagcc aagtcagaat
    24841 gcaacaaatg cttcctatca aagaattgga tgcagaagag gcttctgagg atagagtgat
    24901 tactggtttt gaacttgagc caaaccgtga agttatattg gagcaattat tgcctcaata
    24961 tactgagagt ttgatttatg gagctattat tgatgctaaa acagcagagc atgcggctgg
    25021 tatgacagct atgcagactg caaccgataa tgctaaaaat gttatcaatg atttaactat
    25081 tcaatataac cgtgctcgtc aagcagctat cacacaggaa attacagaaa ttgttgcagg
    25141 tgctaatgca cttgaataat taattagtct atagaaaaaa tagtgaacaa aggtatttta
    25201 aatcctttgt atcatatgat aaggagaaat acatgagctc aggcaaaatt gctcaggtag
    25261 ttggacctgt cgttgacgtt gtatttgcaa gtggcgacaa acttcctgag attaataatg
    25321 cattgattgt ctataaaaat ggcgataagt cacaaaaagt agtacttgaa gttgctcttg
    25381 aacttggtga cggcctcgtt cgtacaatag ctatggaatc aactgatggg cttacacgtg
    25441 gtttggaagt attagatact ggtcgcgcaa ttagtgtgcc ggttggtaag gatactttgg
    25501 gtcgtgtctt caacgttctt ggagatgcta ttgaccttga agagcctttt gcagaagatg
    25561 cagaacgtca accaatccat aaaaaagcac catcgtttga tgaattatca acatcatcag
    25621 aaatcttaga aacaggtatt aaagttattg acttattagc accttactta aaaggtggta
    25681 aagtcgggct tttcggtggt gcgggtgttg gtaaaaccgt tcttattcaa gaattaatcc
    25741 acaacatcgc ccaagaacat ggtggtattt cagtatttac tggtgtagga gaacgtactc
    25801 gtgaagggaa tgacctttat tgggaaatga aagaatctgg cgttattgaa aaaacggcta
    25861 tggtctttgg tcaaatgaat gaaccaccag gagcacgtat gcgtgtggca cttactggtc
    25921 ttacaatagc tgaatacttc cgtgatgtag aaggacaaga tgtgcttctc ttcattgata
    25981 atatcttccg tttcacacaa gcggggtcag aagtgtcagc gcttttaggt cgtatgcctt
    26041 cagccgttgg ttatcaacca acacttgcta cagaaatggg acaattacaa gagcgtatca
    26101 cttcaactaa aaaaggttct gttacatcaa tccaagctat ttatgtgcca gcagatgact
    26161 atactgaccc tgcgccagct acagcctttg ctcacttaga ttcaacaact aaccttgaac
    26221 gtaaattgac acaaatgggg atttatccag ccgttgaccc attagcttca agttctcgtg
    26281 cactcacacc agaaatcgtt ggagatgagc actatgaagt tgcaacagaa gtacagcgtg
    26341 ttcttcaacg ttaccgtgag ttacaagata tcattgcgat tttaggtatg gatgaattat
    26401 cagatgagga aaaaacgtta gttggacgtg cacgtcgtat ccaattcttc ctttctcaaa
    26461 acttcaatgt tgcagaaaca tttactggtc aaccaggttc atatgttccg gttgaagaaa
    26521 ctgttcgcgg attcaaagaa atcctagatg gtaaacatga tcaaattcca gaagatgcct
    26581 tccgtatggt tggtggtatt gaggatgtta tcgcaaaagc agaaaaaatg aactattaat
    26641 gaggtgatgc tatggcacaa ttgactgttc aagtagtaac atcagatggt attagatatg
    26701 accatcacgc tagtctcatt actgttagaa cgccagatgg tgaaatggga attttaccag
    26761 gtcacataaa tttgattgct ccccttattg ttcatcagat gaagattaat cgttctcatc
    26821 aagaaggtgt agactgggta gctgttaatg gtggtataat tgaagttaat gaggatcagg
    26881 tgacaattgt tgcggattca gcagaacgag cacgtgatat agaccttaac cgtgcagaac
    26941 gggctaagga acgcgctgag cgtgcattag aaaaagctca aacaactcag aatattgatg
    27001 agatgcgtag agctgaggtt gctcttcgtc gcgcaattaa ccgtattagc gtcggtaaaa
    27061 agtagaaaaa tggtctaatt agttttgtga ctaattaggc ttttttagtg tataatagaa
    27121 ggcatgaata tgatattaaa tatagtaact atagttagta attttatttt tattgccctt
    27181 ttttatcaac tttttgtaga tttatttgac tggtctaaaa tgattaaaat gtcccctcaa
    27241 aacatctcaa aattaaaagt ttttattttg ttgataagta tagttggtgg ctatgtggtt
    27301 agtcattttt tattggaagt gattcagttg tgtcaatcaa tcttttgggc tctccaataa
    27361 aggaaatatt agctgaacta tgatataatc aacttactat tgttataaga atggagattc
    27421 aaatggataa aattattgtg gaaggtgggc aaacgcggct tcaaggtcaa gttgttattg
    27481 aaggagctaa aaatgcagtt ttacctcttt tagcagccac aattttacca agccaaggga
    27541 aaacattatt gaccaatgtt cctatcttgt ctgatgtatt tacaatgaac aatgttgttc
    27601 gtggtttaga tattcaagtt gactttaatt gcgataaaaa agaaatctta gtggatgcct
    27661 caggtgatat tttagatgtt gcaccttatg aatttgttag tcaaatgaga gcttctattg
    27721 ttgttttggg acctatttta gctagaaatg gccatgctaa ggtttcaatg ccaggcggtt
    27781 gtacaattgg tagccgacca attgaccttc atcttaaagg tttagaagct atgggagcta
    27841 cgattacaca aaatggtggt gatattactg cccaagctga aaagcttaag ggggctaata
    27901 tatacatgga ttttccctca gtcggtgcca ctcaaaactt gatgatggct gctacacttg
    27961 cttcaggcac aacaacgata gagaatgctg cacgtgaacc agaaattgta gaccttgcgc
    28021 agttactaaa taaaatggga gctaaagtca agggtgctgg tacggaaaca ttaacgatca
    28081 ttggtgttga tgctttacat ggtacagaac atgatgttgt ccaagatcgt attgaagctg
    28141 gaacctttat ggtggcagct gctatgactt ctggaaatgt tctagtaaag gatgctattt
    28201 gggaacataa ccgtccttta atctctaaat tgatggagat gggtgttgaa gtgtcagagg
    28261 aagaagatgg tattcgtgta aaagctgata ctaaaaaatt gaagccagtg actgtgaaaa
    28321 cactccctca tccaggattt cctactgata tgcaagctca atttacagct ctaatggcag
    28381 tagttaatgg cgaatcaact atgattgaga cagtttttga aaatcgtttt caacatttgg
    28441 aagaaatgcg tcgcatggga ttacaaacgg aaattttacg agatactgca atgattcatg
    28501 gtgggagagc cttacaaggt gcaccggtca tgtctactga cttacgagct agtgcagctt
    28561 taatcttggc aggaatggtt gcgcaagggc aaacagtagt tggccaatta acccatttag
    28621 accgtggtta ttatcaattc catgaaaaat tagcggctct tggtgctaat attaaacgtg
    28681 taagtgaggc gtaatatggc atctagtggt tggaaatatg tactaaaaca aatcggtcta
    28741 atagtattag tcatactgtt agccttgctg tttttagcgg ttggattgat gttgggttat
    28801 agtgtttttg gtgacggaga gcatgcttac tctattttat cgctggataa atggcaaaat
    28861 attattggaa aatttttagg aaaatagaaa gagccactat aagttatttg atgcttatag
    28921 tggtttcctt tgagagttaa ttttagcagt agaataatac aatgacaaaa aaacaaaaac
    28981 aaataacagc ttcgattgta actttgcttt tttcattact ggcaataggg ttaggtgaaa
    29041 gtaaatcttt gcctaatgat cacattttaa aacaggtgac agatttaatt attagtaatg
    29101 aatctggcaa aaatacttcc cttaaaaata tatcaggaac ccctagtaga gagttatctg
    29161 aatctgtttt gactagcaat gttaaaaaac aattgggaac aaatatagct tggaaccagt
    29221 caggtgcttt tatcattaat caaaataaaa cagatttgaa tgctaaagtg tcaagtgcac
    29281 catatgctat taatgaaata aagaaggtta ataaccaaat tgttccaact aaagcaaatg
    29341 ccttattaac aaaagcaact cgtcaatatc gaaatcgaga agaaacaggg aatggtagaa
    29401 cttattggaa accagctgga tggcatcaga taaatggatt aaaagggagc tataaccacg
    29461 ctgtggacag aggccattta attggttatg ctttagtagg gagtttaaga ggatttgatg
    29521 cctccacaag taatcctaaa aatattgcca cacaagcagc ttgggcaaac caagctaata
    29581 gcaaccaatc aacagggcaa aattattatg aaactttagt tcgtaaagca ttggatcgtc
    29641 ataaaactgt taggtatcgt gtgactttaa tttatgacag agataatctt ttatcttcgg
    29701 gatcacatat tgaggctaaa tcttcagatg gaagtttaga atttaatgtt tttattccta
    29761 atgtacaatc gggattatta tttgattatg caacaggaaa agtgaagcag acaaagtaat
    29821 ctttatctaa tagtgcttgt taaagactga aaaatttgat aaaatagtcc ttaattgaat
    29881 agaggtctgt gagactagta aaccaaggga agctgcttag ggagttcagc cgtgactgca
    29941 agctgaatca gtgaaagagg gtggaattca ctcacaaata ggactataat taaggtctga
    30001 ttggtcgatc agatagaaaa cggatggtac cgcgtgtcaa cgctccgatt aagggggagt
    30061 ggcacgcttt ttttgttttc aaagctactt attttgactt ggaggaaaga tggatttaca
    30121 aaaacaatta gaagagttga aaatatcaac tcaagaaaag cttaaagaaa tgactgggaa
    30181 ccacactaaa gaattacaag acttacgtgt acaagtttta ggaaaaaaag gttctctaac
    30241 agaattacta aaaggtctga aagatttatc taatgattta cgaccagttg ttggaaaaca
    30301 agtcaatgaa gtacgtgata ttttaacaaa agcgtttgag gaacaagcta aagtagtgga
    30361 agctgcgaag attcaagcac agttagagtc tgagagtgtc gacgttacct tgcctggtcg
    30421 tcaaatgaca ttaggtcacc gtcatgtctt aacacaaact agcgaagaga ttgaagatat
    30481 ctttttaggt atgggattcc aagtggttga tggttttgaa gttgagaaag actactataa
    30541 ttttgaacga atgaatttac caaaagatca tccagcccgt gatatgcaag atacttttta
    30601 tattacagaa gaaatcttgc tacgtactca tacttcgcct gttcaagcac gaacaatgga
    30661 ccaacatgat ttttctaaag ggcctttaaa aatgatttct cctggacgtg ttttccgtcg
    30721 tgatactgat gatgctactc atagccatca attccatcaa attgaaggct tagttgttgg
    30781 agaaaatatt tcaatgggcg atctcaaagg aacacttcaa ctgatttctc aaaaaatgtt
    30841 tggagcagag cgtaaaatcc gacttcgtcc ttcatatttc ccattcacag aaccttcagt
    30901 tgaagttgat gtctcttgct tcaagtgcgg aggtaaggga tgtaatgtct gcaaacagac
    30961 aggttggatt gagatattgg gtgctggtat ggttcaccca agtgttttgg aaatgtctgg
    31021 tattgattct gagaaatact ccggttttgc ctttggctta ggtcaagaac gtattgcaat
    31081 gttacgctac ggtatcaatg acattcgtgg cttctatcaa ggtgatgttc gcttcacaga
    31141 tcaattttaa gattttagat agcaagtagc atcattggtg tgatgataaa tttatgttaa
    31201 cgctattacc ttactcaata ggaggttttc taatgtctca aattactttt aaaaaggttg
    31261 gccttgataa tgtgaatatt ttacagaatc ttgctataga aacctttcgg cagacgtttt
    31321 ctcatgataa tagtgaggaa caacttcaag catttttcaa tgagtcttac actttaccag
    31381 tattaaaatc ggaaattacc catgctgaat ctgatactta ttttgtttat cttgatacag
    31441 atttagtggg ttatttaaaa gtaaactggg gaagtcagca aactgaaaaa aacttgataa
    31501 agcatttgaa attcaaagga tttatcttct agatgcctac caagggaaag gtataggtaa
    31561 ggctactttc gaatttgctt tggatttagc ttataaatca ggcttagatt gggcttggtt
    31621 aggtgtttgg gagtttaatc ataaagctca ggcattttac gctaaatacg gatttgaaaa
    31681 attttctgag caccaatttt cagtaggtga taaagtggat acggattggt tattaagaaa
    31741 aagtttacac taaaaactac aaagcacttg tataaaaata aaagaaaata aatagagaga
    31801 aattacatgt tagtatcgta taaatggtta aaagagttag tggatgttga tgtcacaaca
    31861 gcagaactag ctgagaaaat gtcaaccact ggtattgagg ttgaaggtgt ggagacacca
    31921 gcggaaggtt tatcaaaatt agttgtaggt catattgttt cgtgtgaaga tgttccagat
    31981 acccacttac atttgtgtca agttgataca gggggcgatg aacttcgtca agttgtctgt
    32041 ggtgctccaa atgtcaagac tggtattaat gttatcgtag cggttcctgg tgctcgtatt
    32101 gcagataact ataagattaa aaaaggtaaa atccgtggta tggagtcact tggaatgatt
    32161 tgttcacttc aagagttggg cttatcggaa agtatcattc caaaagagtt ttctgatggt
    32221 atccaaattt tacctgaggg agctatacct ggtgatagta ttttctctta tcttgactta
    32281 gatgatgaaa ttattgaatt atcaattaca ccaaaccgtg cagatgctct ttcaatgcgt
    32341 ggtgttgccc atgaggtagc agcaatttat gggaaaaaag ttcattttga agaaaaaaat
    32401 ctcattgaag aagcagaaag agcagctgat aagatttctg ttgtcattga atcagataaa
    32461 gttttatctt actcggctcg tattgttaag aatgtaacag ttgcaccaag tccacaatgg
    32521 cttcaaaata aactgatgaa tgctggtata cgtccaatta ataatgttgt tgatgtgaca
    32581 aactatgttc ttttgactta tggtcaacca atgcatgctt ttgattttga taaatttgat
    32641 gggacaacta tagtagcacg taatgctgag aatggtgaaa agttaattac tcttgatggt
    32701 gaagagcgtg acttaatagc tgatgacttg gtcattgcgg taaatgatca accagttgct
    32761 ttggcaggcg ttatgggagg acaatcaact gaaattgata gttcttctaa gacagtggtc
    32821 cttgaagcgg cagtatttaa tggaacaagt atccgcaaaa caagtggaag actcaacctt
    32881 cgttcagagt catcttctcg ctttgaaaaa ggtatcaatt atgatacggt ttcagaggct
    32941 atggattttg cggctgcaat gttacaagaa ttggcaggca gtcaagttct ttctgggcaa
    33001 gttacagaag gagttcttcc aactgagccg gtagaggtat caacgactct tggttacgtt
    33061 aatactcgtt tgggaacaga acttacctat actgatattg aagaagtatt tgaaaaactc
    33121 ggctttgcta tttctggttc agaagtgaaa tttactgtgt tggtaccgcg tcgtagatgg
    33181 gatattgcca ttcaagctga cttagttgaa gagattgccc gtatttatgg ttacgaaaaa
    33241 ttaccaacaa cattgccaga agccggagcg actgcaggag aattaacgag tatgcaacgc
    33301 ttacgtcgta gagtccgtac ggttgctgaa ggagtaggat tatctgaaat tatcacctat
    33361 gctttaacga ctccagaaaa agcagtgcag ttctcaactc aagcaactaa tataactgaa
    33421 cttatgtggc cgatgactgt tgatcgctca gcacttcgcc aaaatgttgt ttcaggaatg
    33481 cttgatacaa ttgcttataa cgttgctcgc aaaaacagta accttgcagt ttatgagata
    33541 ggaaaagttt ttgaacaaac tggtaatcca aaagaagatt taccaactga agtagagacg
    33601 tttacttttg ccttaacagg tctagtagag gaaaaagatt tccaaacaaa agccaagcca
    33661 gttgatttct tctacgccaa aggtattgta gaggcgctct ttattaaatt aaaattagat
    33721 gttacttttg tagcacaaaa aggtttagca agtatgcacc caggtcgtac agcaactatc
    33781 ttattagatg ggaaagagat tggttttgtt ggtcaagtac acccacaaac tgcaaaacaa
    33841 tatgatattc ctgaaactta tgttgctgaa atcaatctat caactattga aagtcaaatg
    33901 aaccaagcgt tgatttttga agatattacg aagtatccgt ctgtatctcg cgatattgca
    33961 ttattacttg cagaatctgt tagtcatcat gatattgtgt tagcaattga gtcatcaggg
    34021 gtaaaacgtc taacagcgat taaactgttt gatgtctacg ctggaaataa tattgcagaa
    34081 gggtataaat caatggctta tagcttaact ttccaaaacc ctaatgacaa tctaacggac
    34141 gaagaagtgg ctaaatatat ggaaaaaatc actaaatcac tcgttgagaa agttaatgca
    34201 gaaataagat aatttatatc ttacgttgaa aaataaataa aaaacagcat caattaagat
    34261 gctgtttttt tatttaaaat ttatagatgt tctgcggaga aagtatcacc gtgttggatg
    34321 tcaccatatt gaaagccttt gttaaaccag cgttggcgtt gttgagctgt tccatgggta
    34381 aagctatcag gcactaattt tccgtaggtt tctttctgaa gggtatcgtc tccgacggcg
    34441 tgggcagcat tcatggcctc ttcaaagtct ccttgttcta agagattttt tcccctgatg
    34501 tagtgagccc atacccctgc ataataatct gcttgaagtt ctagccgaac atttaaagca
    34561 tttgcttctt tcttagtaag tccgtgtcgc attctattat acttatccat aatgcctaac
    34621 tctgtttgaa tgtggtgacc aacttcatgg gcgatgacgt aggccatagc aaaatcacca
    34681 gtagcaccat atttatgtga taattcattg taaaaagaaa tatcaagata gattttttta
    34741 tctgctgaac aataaaatgg ttctgaagca gattcaccta taccacaacc tgtttgaatc
    34801 gaattggtgt aaagaacaag ttttggttcc ttataatttc caaaaccttg ggtttggaat
    34861 tcttgtgacc agaaatcctc agttgatcca aggactttat taacgaaatc gatttgttct
    34921 ctcgttgcgc tattatcaac agaacgtgag acattctgag attggtaact agaaggtgag
    34981 gatgagtcat taaaaatgct ggttagtccc cctccgccaa gtagcagtaa gatgattaaa
    35041 accacaagct tggttttcca actccctcgc atcaataaaa gttgaagaat cggtaatcca
    35101 cttcctccgc tagagaatga acctccgcta ctggagcgac gatcttcaac attgtcgctt
    35161 tttcttaggt catctatttt cataagtatc tccaaagtgt tttataggct cattataaac
    35221 tattttaata agaaatgctt gaatttttaa ctaaatatct tcccctagat ttgaagctat
    35281 ttttggtata atattaataa actgtaaaga ataagttgaa gaaggtttat ttctcagttg
    35341 acttataaga taggagattc atgaaattac tttatactga tattaatcat gatatgacag
    35401 aaatcttagt caatcaggcc gcgcatgctg ctgaagctgg atggcgtatt ttttatattg
    35461 ctccaaactc tttatctttt gaaaaggagc gtgcagtatt agagaccctt cctcaagaag
    35521 cctcttttgc tattacaatt actcgctttg ctcaattggc acgttatttt actttaaacc
    35581 agcctaatca aaaagaaagt ttaaacgata ttggattagc aatgatcttt tatcgtgctt
    35641 tagcaagttt tgaagatgga caattaaaag tttttggacg attgaagcaa gatgcttcct
    35701 ttatcagtca attggtagac ctatacaaag aattgcagac agcaaatctg agtattttag
    35761 atttaaaata tttacattct cctgaaaaat ttgaagatct attagctatt tttctagtgg
    35821 tttcagatct tttaagagaa ggagaatacg acaatcaatc taaaattgcc ttttttacag
    35881 aacaagtaag gtcaggacag ctagatgtgg atttaaagaa cactatcctt atagtcgatg
    35941 gttttacccg tttctcagct gaagaagaag ctttaattaa aagtctgagt tcacgttgtc
    36001 aagagattat catcggagct tatgctagcc aaaaagcgta taagactaac tttaccaatg
    36061 gaaatattta ttctgcaggg gttgattttt taagatatct agcaacgaca tttcaaacaa
    36121 aaccagaatt tatcttgtct aagtgggaaa gtaagtctgg ttttgaaatg atttcgaaaa
    36181 atatcgaagg taaacatgat tttacaaata gtagtcacat tttagacgac actgctaaag
    36241 attgtataac aatttgggag tgcattaatc aaaaagatga agtggaacat gtggctagag
    36301 ctatacgtca gaaactttat cagggatatc gctacaaaga tattttagtt ttactaggag
    36361 atgtagatag ttataaacta caattaagta agatttttga acagtatgat attccttatt
    36421 atttcggtaa agctgaaaca atggctgcgc accctttagt tcattttatg gattccttgt
    36481 cgcgaataaa acgttatcga tttagagctg aagatgtttt aaatcttttt aagacaggta
    36541 tttatggaga gattggtcaa gatgatttag attattttga agcttatatc tcatatgcag
    36601 atattaaagg ttctaagaag ttctttacag attttgttgt gggggctaaa aaatttgatt
    36661 tagggcgtct taacactatt cgtcagtccc tccttactcc actggaaagt tttgttaaaa
    36721 cgaaaaaaca agacggtata aaaacactca accaattcat gtttttcctt actcaggtag
    36781 gtttgagtga caatttatcg cgattagttg gtcaaatgtc tgaaaatgaa caagaaaaac
    36841 atcaagaagt ttggaaaaca tttacagata ttttagagca gtttcaaaca atatttggtc
    36901 aagaaaaatt aaatttagac gaatttttat cgttattaaa ttcaggtatg atgcaagccg
    36961 agtatcgtat ggtccctgct actgttgatg tggttactgt taagtcttac gacttggtag
    37021 aaccacatag taatcaattt gtctatgccc taggcatgac acaatcacat tttccaaaga
    37081 ttgctcaaaa caaatcttta atatctgata ttgaacgtca gcttataaat gatgccaatg
    37141 acactgatgg tcattttgac attatgacgc gagaaaattt gaagaaaaac cattttgctg
    37201 ctctctctct ttttaatgct gctaaacaag cattagtgtt gactatacca caattattga
    37261 atgagtctga agatcagatg tcaccatatc ttgtcgaact aagagatata ggtgttcctt
    37321 ttaatcataa aggacgccaa tcgttaaaag aagaagctga taatattggg aattacaaag
    37381 ctttattatc gcgtgttgtt gacttgtatc gctctgctat tgacaaagag atgactaaag
    37441 aagaacaaac tttttggtca gttgctgttc gttatttgcg tcgtcaatta acttctaaag
    37501 ggatcgagat accaataatt acagatagtc ttgatactgt gacagtttca tcagatgtga
    37561 tgaccagacg cttcccagaa gatcacccac ttaaattatc ctcatctgct ttgacaactt
    37621 tttataataa tcaatataag tattttttac aatatgtgtt aggtttggaa gaacaagact
    37681 caattcatcc agatatgcgg caccacggaa cgtatcttca tcgtgttttt gaaattctga
    37741 tgaaaaacca agggatagaa tcttttgaag agaaactcaa ctctgcaatt aataaaacca
    37801 atcaagaaga tgttttcaaa tccttgtatt cagaagatgc agaaagtcgt tattctttag
    37861 aaattttaga agatatcgcg cgtgcaactg caaccatatt acgacaagat agtcagatga
    37921 ctgttgaaag tgaagaagaa cgatttgaac taatgattga taatactata aaaattaacg
    37981 gtattattga ccgtatcgat cgtttatctg atggttcttt gggtgtcgtg gactataaat
    38041 caagcgctca aaaattcgat atccaaaaat tttataacgg tctaagtcca caattagtca
    38101 cctacattga tgctatcagt cgtgacaagg aagttgaaca aaaaccaccg atttttggag
    38161 ctatgtactt acatatgcaa gaacctagac aagacttgtc taaaattaaa aatttagatg
    38221 atttagtaac aaaaaatcat caagctttaa cttataaagg attattttca gaagccgaaa
    38281 aagaattctt agcaaatggg aaataccatc tcaaagattc cctttattca gaggctgaaa
    38341 ttgctatttt acaagcacat aatcaatcgc tttacaaaaa agcatctgag actattaagt
    38401 caggtaagtt tttgatcaat ccatatacag aagatgctaa aacagttgac ggtgatcagt
    38461 ttaagagtat tacggggttc gaggcggata ggcatatggc acgtgccaga gcgctttata
    38521 aattacctgc taaggaaaaa cgtcaaggtt tcttaacatt aatgcagcaa gaggaggaaa
    38581 atgatgacct ttaagccttt cttaaatcca gaagatattg ctgttattca gacagaagaa
    38641 aaaaattctg ataaaaaaca aaagcgaacg cctgaacaaa ttgaagcaat ttatactttt
    38701 ggtaacaatg tcctggtatc agcttcggct ggttctggaa agacttttgt aatggttgag
    38761 cggattttag ataaactttt aagaggggtt ccgattgagt ctttgtttat ctcaacattt
    38821 acagtaaaag cggcaggtga gctcaaagaa cgtttagaaa agaaaataaa cgagtctttg
    38881 aaatccgcag aatcagatga tttaaaacag tttctcaccc agcaacttgt cggcattcaa
    38941 acagctgata ttggtactat ggatgctttt acgcaaaaaa tagttaacca atatggttat
    39001 actttgggca tatcacctat atttaggatt ttacaagata aaaatgagca agacgtcata
    39061 aagaatgagg tatatgctga tttattttct gactacatga ctgggaagaa tgcagcttca
    39121 ttcataaaat tagtcaaaaa tttctcaggt aatcgtaaag atagcaaagc ttttcgagaa
    39181 atggtttata aagtttatgc ttttagccag tccatcgata accccaagcg ctggatgcaa
    39241 acagtttttc taaaaggtgc acaaacttac actgattttg aagctattcc agatcaagaa
    39301 gtttcaagcc ttttaaatgt aatgcaaaca actgctaatc aattacgtga tttaacggat
    39361 caagaagatt ataagcaatt aactgctaaa ggggttccga cagcaaatta caaaaaacac
    39421 cttaaaataa ttgaaaattt agtccattgg tctcaagact ttaatttgct gtatggaaaa
    39481 aaaggattgg ctaatttggc acgtgatatc actaatgtga ttccttcagg caatgatgtt
    39541 acagttgctg gcgttaaata tcctatcttt aaacaattgc ataatcgtat cgttggactt
    39601 aagcacttag aagttatttt taagtatcaa ggagaatctc ttttcttgtt agaattattg
    39661 caatcctttg ttttagattt ctcagaacaa tatttacagg agaagattca agaaaatgct
    39721 tttgaatttt ctgatattgc ccattttgca attcaaatat tggaagaaaa tcatgatatt
    39781 aggcaaattt atcaagataa gtatcatgaa gtaatggttg atgagtatca ggacaataac
    39841 catacccaag agagaatatt agaattattg tctaatggac ataatcgttt catggttgga
    39901 gacataaaac aatcaattta ccgttttcgt caggctgatc cacaaatttt taatgataaa
    39961 tacaaagctt accaagacaa tccaagtcaa gggaaattaa ttattctgaa agagaatttt
    40021 cggagtcaat cagaggtttt agacagtaca aatagtgtgt ttacacatct tatggatgaa
    40081 gaagtaggag acattcttta tgatgaaagt caccaattaa aagctggtag tccaagacag
    40141 caagaaagac atccgaataa taaaacacaa gttttactat tagatactga tgaagatgat
    40201 atagatgaca gtgatagtca acaatatgat attagcccag ctgaagcaaa gttagtcgca
    40261 aaagaaatta ttcgattgca taaagaagag aatgttccct ttcaagatat caccctctta
    40321 gtttcttcaa ggacaaggaa tgatggtatc ttacaaactt ttgaccgtta tggtattcct
    40381 ttggtaactg acggtgggga gcaaaattac ttaaaatcag tcgaagtgat ggtaatgctt
    40441 gatactttgc gatcaattga taatccctta aatgattatg ctctagttgc tcttttgaga
    40501 tctcccatgt ttggctttaa tgaagatgat ttaacaagaa ttgctattca tgatgtcaaa
    40561 atggcttttt atcataaagt gaaattgtct tatcataaag aaggtcatca tagtgattta
    40621 attactccag aattatcttc taaaatagat tactttatga agacattcca gacttggcgt
    40681 gattttgcta aat
//
LOCUS       DATROD010000009        37261 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.009, whole genome shotgun sequence.
ACCESSION   DATROD010000009 DATROD010000000
VERSION     DATROD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37261
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.009"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..363)
                     /locus_tag="VBC74_000468"
     CDS             complement(<1..363)
                     /locus_tag="VBC74_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6941442.1"
                     /translation="MITYDFTSLPERFSSNAIKWKAVQKDQEILPLWIADMDFPIFPE
                     MSEAIEDFSHQMVFGYDSPKDSLYQAISNWEVQEHGYQFDKKSLLLIDGVVPAISVAI
                     QAFTKEGDAVLINTPVYPP"
     gene            complement(499..1047)
                     /locus_tag="VBC74_000469"
     CDS             complement(499..1047)
                     /locus_tag="VBC74_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEO6941443.1"
                     /translation="MNIETLTQKNHRSDSGRNHIVLFEPQIPANTGNIARTCAATNAP
                     LHIIRPMGFPIDDKKMKRAGLDYWDKLDVSFYDGLEEFMLSCRGKVHLISKFADKVYS
                     DENYNDDQDHYFMFGREDKGLPETFMREHAEKALRIPMNDEHVRSLNVSNTVCMIVYE
                     ALRQQSFPNLELSHTYENDKLK"
     gene            complement(1220..2572)
                     /locus_tag="VBC74_000470"
     CDS             complement(1220..2572)
                     /locus_tag="VBC74_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEO6941444.1"
                     /translation="MENHNSIKQTYGLMTTIAMIVGVVIGSGIYFKVDDILKFTGGDV
                     FLGMVILVLGSFSIVFGSLSISELAIRTSESGGIFSYYEKYVSPALAATLGLFASFLY
                     LPTLTAIVSWVAAFYTLGESSSLESQIILAAVYILALSLMNIFAKRIAGGFQSLTTFV
                     KMIPLVLIALIGAFWSDKAPQLPQHLTAIQPSNVGWSWVSGLVPLYFAYDGWTIFVNI
                     APEVKNPKKNLPLAFVIGPALILLSYLAFFYGLTQILGASFIMTTGNDAINYAANIIF
                     GPSVGRLLSFIVILSVLGVANGLLLGTMRLPQAFAERGWIKSERMANINLKYQMSLPA
                     SLTVTAVAIFWLFVHFMVTKFNLLPGSDISEIAVVFNNTSLIILYVLVLSLYLKKDIK
                     NKFTGLVSPILAILGGLILLIGSLLSNFFTVLIFQCFCLLFCLICHYIYQKNNPKTHE
                     "
     gene            2778..4127
                     /gene="trkA"
                     /locus_tag="VBC74_000471"
     CDS             2778..4127
                     /gene="trkA"
                     /locus_tag="VBC74_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEO6941445.1"
                     /translation="MRIIVVGGGKVGTALCRSLVAEKHDVVLIEKKENVLKRVTKQHD
                     IMGIVGNGANYKILEQAEVKNCDIFIAITDRDEVNMISAVLAKKMGAKETVVRMRNPE
                     YSNPYFKDKNFLGFSSVVNPELLAAQYIANTIEFPNATSVEHFANGRVMLMEFKILEG
                     NKLCHTSMSQIRKKFGNIVICAIERDGKLIIPDGDATIQVKDKIFVTGNRIEMILFHN
                     YVKNKVVKNLMVIGAGRIAYYLLNILKNTNTHVKLVELNQEQAEYFSQEFPNVPVVHG
                     DGTAKNILLEESVTNFDAVATLTGVDEENIITSMFLESIGIPKNITKVNRTSLLEIID
                     DKQLSSIITPKRIAVDHVMHFVRGRVNAQDSNLEAMHHIANDRIETLQFEIKETSKLA
                     NRSLASLKLKQNILIAAIIRNNKTIFPTGEDVLTVGDRIVVITLLKNITRTSDMLAR"
     gene            4132..5571
                     /locus_tag="VBC74_000472"
     CDS             4132..5571
                     /locus_tag="VBC74_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO6941446.1"
                     /translation="MNKSMIRFLLSKLLLIEAALLAIPLTVGLIYREPQSVMMSIVIT
                     MIILIILGLLGSLFKPKNYHIYTKEGMLIVALCWILWSFFGALPFVISGQIPNIIDAF
                     FEVSSGFTTTGATILDDVSVLSPALLFWRSFTHLIGGMGVLVFALAIMENSKNSHLEV
                     MRAEVPGPVFGKVVSKLKKTAQILYLLYLLMFAVFAVILYFAGMPFFDSIIIAMGTAG
                     TGGFAVYNDSIAHYNSPLITNLVSIGMLIFGVNFNLYYLLLLRKIKAFFGDEELKTYL
                     RIVAIATFMIALNVIGMYDNFRQGLEHIFFEVSAIITTTGFGVTDITRWPLFSQVILL
                     FLMFIGGSAGSTAGGFKVMRSLILAKIARNQVLSTLYPNRVMSLHINKSVLDKNTQHG
                     VLKYLTIYLAIFMALVLVLTLDTNDFLVVISAAASCFNNIGPLLGSNETFSFFSPFSK
                     LLLSFAMIAGRLEIYPVLLMFIPKTWSKL"
     gene            complement(5595..5846)
                     /gene="yidD"
                     /locus_tag="VBC74_000473"
     CDS             complement(5595..5846)
                     /gene="yidD"
                     /locus_tag="VBC74_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEO6941447.1"
                     /translation="MLKSFLIFLVRFYQKNISPAFPASCRYRPTCSTYMIEAIQKHGL
                     KGVLMGIARILRCHPLAHGGNDPVPDHFSLRRNKTDISD"
     gene            complement(5846..6568)
                     /locus_tag="VBC74_000474"
     CDS             complement(5846..6568)
                     /locus_tag="VBC74_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO6941448.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGMVTINGQVVNELATQVKAG
                     DLVEIEGSPIYNEEKVYYLLNKPRGVISSVSDDKGRKTVIDLLPQVKERIYPVGRLDW
                     DTTGLLILTNDGDFTDKMIHPRNEIDKVYLARVKGIATKENLRPLTRGVVIDGKKTKP
                     ARYTIIKVDHEKNRSVVELTIHEGRNHQVKKMFEQVGLLVDKLSRTQFGTLDLTGLRP
                     GEARRLNKKEISQLHNAAINKS"
     gene            complement(6558..7142)
                     /locus_tag="VBC74_000475"
     CDS             complement(6558..7142)
                     /locus_tag="VBC74_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEO6941449.1"
                     /translation="MTYLGSIEALLFVAGEDGLSLRQMAELLSLTPSALIQQLEKLAK
                     RYEEDDDSSLLLLETAQTYKLVTKDSYMTLLRDYAKAPINQSLSRASLEVLSIIAYKQ
                     PITRIEIDDIRGVNSSGAITRLIAFGLIKEAGKKEVLGHPNLYETTNYFLDYMGINQL
                     DDLIDASSIELVDEEVSLFSMDSINTEDKENNEN"
     gene            complement(7139..7846)
                     /locus_tag="VBC74_000476"
     CDS             complement(7139..7846)
                     /locus_tag="VBC74_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEO6941450.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYEVDIYDVPIVEVIEQYLAYIAT
                     LQAMRLEVAGEYMLMASQLMLIKSRNLLPKVVESNPIEDDPEMELLSQLEEYRRFKVL
                     SEELANQHQERAKYFSKPKQEVIFEDAILLHDKSVMDLFLTFSQMMSQKQKELSNSQT
                     VIEKEDYRIEDMMIVIERHFNLKKKTTLQEVFADCQTKSEMITLFLAMLELIKLHQIT
                     VEQDSNFSQVILRKEEK"
     gene            complement(7846..8586)
                     /gene="xerD"
                     /locus_tag="VBC74_000477"
     CDS             complement(7846..8586)
                     /gene="xerD"
                     /locus_tag="VBC74_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEO6941451.1"
                     /translation="MINDINNFIESKKLSLNSRKSYHYDLKQFYKIIGGHVNSEKLAL
                     YQQSLSEFKLTARKRKLSAVNQFLFFLYNRGTLKEFYRLQETEKITLAQTKSQIMDLS
                     NFYQDTDYPSGRLIALLILSLGLTPAEIANLKKADFDTTFNILSIEKSQMKRILKLPE
                     DLLPFLLESLEEDGDLVFEHNGKPYSRQWFFNQLTDFLNEKNEQQLTAQLLREQFILK
                     QKENGKTMTELSRLLGLKTPITLERYYR"
     gene            complement(8576..9049)
                     /locus_tag="VBC74_000478"
     CDS             complement(8576..9049)
                     /locus_tag="VBC74_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO6941452.1"
                     /translation="MIAKEFESFLLNHLDHYLIPAEDVAIFVDTHNADHVMLLLASNG
                     FSRVPVITKEKKYVGTISISDIMGYQSKGQLTDWEMAQTDIVEMVNTKIEPINEAATL
                     TAIMHKIVDYPFLPVISDQNDFRGIITRKSILKAINSLLHDFTDEYTITPKNNDK"
     gene            complement(9046..9567)
                     /locus_tag="VBC74_000479"
     CDS             complement(9046..9567)
                     /locus_tag="VBC74_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEO6941453.1"
                     /translation="MAIRKLVVMSDSHGDRDIVKDIKNHYLGKVDAIFHNGDSELPSS
                     DPIWEGIHVVTGNCDYDSGYPEVLVTKIDNAVIVQTHGHLHQINFTWDKLDLLAQQED
                     ADICLYGHLHRADAWKNGKTIFINPGSVLQPRGPINEKLYAVVTITDSKVLVEYYTRQ
                     HQPYPNLTKEFSR"
     gene            complement(9549..10523)
                     /locus_tag="VBC74_000480"
     CDS             complement(9549..10523)
                     /locus_tag="VBC74_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEO6941454.1"
                     /translation="MTKTIFESKTEGNWFLGSFQAFNYFTCFGNDESYEAIQDVFHRL
                     LSTLKVEGLQLHVVQMTSDFQLLAFLVDMINQEYSRHIKVTQHKGAILVSEDDQLFLV
                     HLPKEGTSLEKFFDLKNDNNFGDTILIATHNEGKTKEFRELFGKLGLKVENLNDYPDL
                     PEVEETGMTFEENARLKAETISKLTGKMVISDDSGLKVDALGGLPGVWSARFSGPDAT
                     DARNNAKLLHELAMVFDKERRSAQFHTTLVVSAPNKESLVVEAEWPGYIGTEPKGENG
                     FGYDPLFIVGEGSRTAAELSAQEKNNLSHRGQAVRKLMEVFPKWQLEN"
     gene            complement(10520..11314)
                     /gene="racE"
                     /locus_tag="VBC74_000481"
     CDS             complement(10520..11314)
                     /gene="racE"
                     /locus_tag="VBC74_000481"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEO6941455.1"
                     /translation="MDSRPIGFLDSGVGGLTVVKEMFRQLPEEEVIFIGDQARAPYGP
                     RPAQQIREFTWQMVNFLLTKNVKMIVIACNTATAVAWQEIKEKLDIPVLGVILPGASA
                     AIKSTNLGKVGIIGTPMTVKSDAYRQKIQALSPNTAVVSLACPKFVPIVESNQMSSSL
                     AKKVVYETLSPLVGKLDTLILGCTHYPLLRPIIQNVMGAEVKLIDSGAETVRDISVLL
                     NYFEINHNWQNKHGGHHFYTTASPKGFKEIAEQWLSQEINVERIVL"
     gene            complement(11489..11728)
                     /locus_tag="VBC74_000482"
     CDS             complement(11489..11728)
                     /locus_tag="VBC74_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEO6941456.1"
                     /translation="MSTTIWILLIIVALFGGLVGGIFIARKQIEKEIGEHPRLTPDAI
                     REMMSQMGQKPSEAKVQQTYRNIVKHAKTAIKTKK"
     gene            complement(11886..12428)
                     /locus_tag="VBC74_000483"
     CDS             complement(11886..12428)
                     /locus_tag="VBC74_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941457.1"
                     /translation="MSKKTTQMVSYTSILVAFAIMIPIIMPAKIIIGPASFTLASHVP
                     LFLSIFMSVPVAILVALGTGLGFLLAGFPIVIVLRALSHIGFALIAAFLIKSKPSLLM
                     SKWQTLLFAVAINIIHGLLEFITVYIITMTSNSSSTYLWSLFSLIGLGSLLHGLVDFY
                     IALFIWKWMTQKLGKTLRIR"
     gene            complement(12490..13011)
                     /locus_tag="VBC74_000484"
     CDS             complement(12490..13011)
                     /locus_tag="VBC74_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEO6941458.1"
                     /translation="MKAQERRENILTTLKGTKEAISASTLAKIFSVSRQVIVGDIALL
                     RAQQCDIISTPKGYLMSSALSTHQFTARLVCQHGIEQTEEELEIILRYQGIIMNVEVE
                     HPIYGMLTAPLNIQSQKDIDNFTAKLKVSNAELLSSLTDGLHTHMISCQDQSVFDQIC
                     EALKKAGILYIDK"
     gene            complement(13079..13768)
                     /locus_tag="VBC74_000485"
     CDS             complement(13079..13768)
                     /locus_tag="VBC74_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEO6941459.1"
                     /translation="MNDNVIYTQSDSGLNQFFAKIYGLVGIGVGLSAAVSAIMLYMFP
                     QNMIAIMQKMPGLYFGAIILELVLVFVASGAARRNTPAALPLFLIYSALNGFTLSFII
                     ARYTQTTVLQAFITSAAVFFAMALIGAKTKKDLSGMRKALMAALIGILIASLVNLFIG
                     SGGMSYIMSIVCVIIFSGLIAYDNQMIKYVYNSQGGQVADGWAVSMALSLYLDFINLF
                     LNILRLFARND"
     gene            complement(13791..14294)
                     /locus_tag="VBC74_000486"
     CDS             complement(13791..14294)
                     /locus_tag="VBC74_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO6941460.1"
                     /translation="MKKYQDDKDFMDLVGHLIDHPRFQKLEAIVQHHHSTRLEHSINV
                     SYTSYKIAKKFGWDASSTARGGLLHDFFYYDWRVTKFNKSHAWVHPRIAVRNARKLTD
                     LNAREEDIILKHMWGATIAPPRYKESYIVTMVDKYWAVREASRPLKRIFKKPIRFSRK
                     FLGSHNH"
     gene            complement(14332..15075)
                     /locus_tag="VBC74_000487"
     CDS             complement(14332..15075)
                     /locus_tag="VBC74_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEO6941461.1"
                     /translation="MTTIITSKSNNLIKKTKKLLQKKYRKSSYLIEGWHLFEEAEKYG
                     AQFLNIFVTETAIDRLRKPERAIVVTDDVLKELTDSQTPQGIVAEIAFQETRWTDIKK
                     GRFLVLEDVQDPGNLGTMVRTADAANFDAVFLSQKSADLYNQKTLRSMQGSHFHLPVF
                     RVEIEQFVNFCKAEGITMIATTLSEQSVNYKNLPKYDYFALIMGNEGQGISKTMTEEA
                     DVLAHIEMPGQAESLNVAVAAGVVIFSLI"
     gene            15107..15385
                     /locus_tag="VBC74_000488"
     CDS             15107..15385
                     /locus_tag="VBC74_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEO6941462.1"
                     /translation="MKKVHLIVSGRVQGVGFRYATYSLALEIGDIYGRVWNNDDGTVE
                     ILAQSTDSNKMTQFIQKIRKGPSKWSKVTYVDIKLDNFDDFNDFKMSN"
     gene            15470..16402
                     /gene="yidC"
                     /locus_tag="VBC74_000489"
     CDS             15470..16402
                     /gene="yidC"
                     /locus_tag="VBC74_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO6941463.1"
                     /translation="MKKTLKRILFSSLSLSMLLLLTGCVSVDKAGKPYGVIWNTLGVP
                     MANLITYFAQHQGLGFGVAIIIVTVIVRVVILPLGLYQSWKASYQAEKMAYFKPLFEP
                     INERLRNAKTQEEKLAAQTELMTAQRENGLSMFGGIGCLPLLIQMPFFSAIFFAARYT
                     PGVSSATFLGLNLGQKSLILTVIIAILYFVQSWLSMQGVPDEQRQQMKTMMYLMPIMM
                     VFMSISLPASVALYWFIGGIFSIIQQLVTTYVLKPKLRRKVEEEYTKNPPKAYKANNA
                     RKDVTNSTKATESNQAIITSKKTNRNAGKQKRRG"
     gene            complement(16602..17267)
                     /locus_tag="VBC74_000490"
     CDS             complement(16602..17267)
                     /locus_tag="VBC74_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6941464.1"
                     /translation="MINWDAIFNLELAVKAFPSVIQGLPYTIGLSLVGFILGAIVGFF
                     VALMKMSHFRLLRYLANIHISLMRGIPLMVLLFLIYFGLPFIGIQLDAVTASIVGFTM
                     MSSAYISEIIRAALLAVDHGQWEAARALGLKTPTIYRGIIIPQATRIALPSLSNVLLD
                     MVKGSSLTAMITVPDIFNNAKIVGGTYSDYMTAYILVALIYWVICTLYAIIQDWWEKR
                     LLY"
     gene            complement(17291..18148)
                     /locus_tag="VBC74_000491"
     CDS             complement(17291..18148)
                     /locus_tag="VBC74_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6941465.1"
                     /translation="MKKTILLGLVGLSAMTLAACSNGQSNKETTWDNIKKDGVLKVAT
                     PATLYPTSYYDDHKKLTGYEIDMMKAIAKKLKIKVKFVEVGVAESFTSVDSGKVDVAV
                     NNFDTTPERLKKYNFSQPYKYSVGGMIVRADGSSKITAKDLSDWKGKKAGGGAGTQYM
                     KIAKQQGAEPVIYDNVTNDVYLRDVSTGRTDFIPNDYYTQVIAVKYVTKQYPDIKVKM
                     GDVKYNPTEQGIVMSKKDKSLKTKIDAAIKDMKKDGSLKKISEKYYAGQDLTKEPAGT
                     DKIPVIKVK"
     gene            complement(18309..19769)
                     /locus_tag="VBC74_000492"
     CDS             complement(18309..19769)
                     /locus_tag="VBC74_000492"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEO6941466.1"
                     /translation="MVFKDATAMVQAIKQHKISSQELVEQAIYKIEEQNVSVNAVVSK
                     QYNEARQAAKYANESNAPFAGVPILLKDLGQNQKGQLSTSGSQLFKHYHAKQTDYLVQ
                     SFEKLGFIILGRTNTPEFGFKNISDGQLHGNVNLPFDHSRNAGGSSGGAAAAVSSGMV
                     PIAGASDGGGSIRIPASFNGLIGLKPSRGRIPVGPSSYRGWQGASSHFALTKSVRDTK
                     RLLYYLQSYQVESPFPLKKLSKESLFEFSVSKPLKIAVLMDSPLKTKVSSEAKAAIKE
                     AADFLSQKGNHLELVEQPLDGIHSMKTYCMMNSVETAAMFDDIEKSLGRSMEFSDMEL
                     MTWAMYQSGQRVLAKDYSKLLDSWDQFAATMARFHENYDLILTAATNQPAPFHGQFDL
                     DETLQKQLRHMGEFSVSEQQDLIWKMFEDSMAWTPFTHQPNLTGQPSLAIPTHLTKEG
                     LPLGVQLTAAKGREDLLLAVAELFEKEKQFKGPVYH"
     gene            complement(19873..20355)
                     /gene="greA"
                     /locus_tag="VBC74_000493"
     CDS             complement(19873..20355)
                     /gene="greA"
                     /locus_tag="VBC74_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEO6941467.1"
                     /translation="MAEKTYPMTQVEKDQLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQIQILETKIRYAEIIDSDAVAKDEVAIGKTVLVQEVGTND
                     KDTYHIVGAAGADIFSGKISNESPIAHALIGKKTGDLATIESPAGSYQVEIISVEKTN
                     "
     gene            complement(20428..22230)
                     /gene="mltG"
                     /locus_tag="VBC74_000494"
     CDS             complement(20428..22230)
                     /gene="mltG"
                     /locus_tag="VBC74_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEO6941468.1"
                     /translation="MTEFNDDQHSNHDQKSFKEQILAELEEANRLRKLREEELYQKEQ
                     EAKEAARRTAQLMADYEAQRLKDEQEARAKALETKQRLEEQEKARIEAKLLAEAAREE
                     ERRQAEQALASQEEQVINQGMEPSRELDSGSKSSEFRTTENVPDIDLKADKTDVATAV
                     PNQETEEIFLVRATDIPTEGENVKLGETSELEPVAKEPIRVEDLSKEEEGIALSAKNK
                     HNKRERRQKADNVAKRIARILISIIILVLLLTAFVGYRFVDSAIKPVDSNSNKFVQVE
                     IPIGSGNKLIGQILEKAGVIKSATVFNYYSKFKNYSNFQSGYYNLKKSMTLDQIAAEL
                     EKGGTAEPTKPALGKILITEGYTIKQIAKAIESNKIDTKTTSTPYKADDFLKLVQDET
                     FIKKMVAKYPNLLGSLPDKSKAIYQLEGYLFPATYNYYKDTTLEGLVEDMISTMNTKI
                     APYYNTIKAKNMSVNDVLTLSSLVEKEGSTDEDRRKIASVFYNRLSAGQALQSNIAIL
                     YAMGKLGDKTSLAEDAQINTSIKSPYNIYTNTGLMPGPVDSPSISAIEATIKPASTDY
                     LYFVADVKTGNVYYAKDFETHKANVEKYINSQIN"
     gene            complement(22317..22820)
                     /locus_tag="VBC74_000495"
     CDS             complement(22317..22820)
                     /locus_tag="VBC74_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6941469.1"
                     /translation="MIIRNGCLEDLQQVISIEQINFSEAEAASKKAMQERLTIMTDTF
                     LVAEINGRLAGYIEGPVIKGRYLTDDLFHKVSEFPVRVGGFIGITSLSIHPDFKGQGI
                     GTALLAAMKDLVVSQERDGISLTCHDDLISFYEMNGFKDEGESDSQHGGSLWYNMIWN
                     NPTRSIC"
     gene            complement(22906..24237)
                     /locus_tag="VBC74_000496"
     CDS             complement(22906..24237)
                     /locus_tag="VBC74_000496"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEO6941470.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHNVQGSDVDKYYFTQRGL
                     EQAGVTILPFSPNNISEDLEIIAGNAFRPDNNEELAYVIEKGYHFKRYHEFLGDFMRQ
                     FTSLGVAGAHGKTSTTGLLAHVLKNITDTSFLIGDGTGRGSANANYFVFEADEYERHF
                     MPYHPEYSIITNIDFDHPDYFTGLEDVFNAFNDYAKQVQKGLFIYGEDPKLHEITSEA
                     PIYYYGFEDSNDFIAKDITRTVNGSDFKVFYNQEEIGQFHVPAYGKHNILNATAVIAN
                     LYIMGIDMALVAEHLKTFSGVKRRFTEKIIDDTVIIDDFAHHPTEIIATLDAARQKYP
                     SKEIVAIFQPHTFTRTIALLDEFAHALSQADSVYLAQIYGSAREVDNGEVKVEDLAAK
                     IVKHSDLVTVENVSPLLNHDNAVYVFMGAGDIQLYERSFEELLANLTKNTQ"
     gene            complement(24247..24864)
                     /locus_tag="VBC74_000497"
     CDS             complement(24247..24864)
                     /locus_tag="VBC74_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941471.1"
                     /translation="MNRGSRRFPLIADDEPVMSPLVKMNLYDNEDLINNIRGFYQEKT
                     YQSMVKSNYEHEEISHPKVIENDPVPPQSFVKKATELSKSRQEAKRSVREKRQAYYAK
                     QEFKAPSKEAFQQQLKATVPKKQTQRKVTELSHLSDRLQQESYILAEIPIIFQEPDNT
                     PNPKTKKNNFDFLKRSQVYNKQDNQFHKERAKAQELNLTRFKDIN"
     gene            complement(25029..28127)
                     /locus_tag="VBC74_000498"
     CDS             complement(25029..28127)
                     /locus_tag="VBC74_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6941472.1"
                     /translation="MSRMIPGRIRNQGIELYEQGLVSLISQEGNLLKAKVGDCQIEYS
                     LVTEETKCSCDFFARKGYCQHLAALEHFLKNDPEGKAILSKVQVQQESQQETKKKTSF
                     GSVFLDSLIINEDDTIKYQLSAQGEQNPYANDIWWTLKIRRLPDDRSYVIRDIKAFLN
                     TVRKEAYYQIGKQYFETLSLIQFDETSQELIEFLWRLIPSHSSKIDLEFILPNQGRHL
                     SLTRGFFEEGVTLMNALENFSFESDFHQFNHLYFKELEGEDHLYQFKVIVHRQSIELE
                     IKEKDLKPLFANSYLFYRDTFYHLNLKQEKMVTAIRSLPIEGNLAKHIHFDLDDQDKL
                     AAHLLDFKEIGLVDAPRSFSIHDFKVNFEFDINSQNEILLQMVFDYGNDLTVHNRQEL
                     EQLTFASHFKHEEKVFKLLEKYGFAPHFSTSHPAYSAQELYDFYTYMLPQFKKMGTVS
                     LSAKLESYRLIERPQIDIEAKGSLLDISFDFSDLLENDVDQALVALFDNNPYFVNKSG
                     QLVIFDEETKKVSATLQGLRARRAKNGHIELDNIAAFQLSELFANQDNVSFSQHFYQL
                     IEDLRHPEKFKIPGLSVSASLRDYQLTGVRWLSMLDHYGFAGILADDMGLGKTLQTIS
                     FLSTKLTRDSRVLILSPSSLIYNWQDEFHKFAPDVDVAVAYGSKIRRDEIIAERHQVI
                     ITSYSSFRQDFETYSEGNYDYLILDEAQVMKNAQTKIAHSLRSFEVKNCFALSGTPIE
                     NKLLEIWSIFQIILPGLLPGKKEFLKLNPKQVARYIKPFVMRRRKEEVLPELPDLIEM
                     NYPNEMTDSQKVIYLAQLRQIQESIQHSSDADLNRRKIEILSGITRLRQICDTPRLFM
                     DYDGESGKLESLRQLLTQIKENGHRALIFSQFRGMLDIAEREMVAMGLTTYKITGSTP
                     ANERHEMTRAFNAGSKDAFLISLKAGGVGLNLTGADTVVLIDLWWNPAVEMQAISRAH
                     RLGQKENVEVYRLITRGTIEEKILEMQETKKHLVTTVLDGNETHASMSVDDIREILGV
                     SK"
     gene            complement(28282..29592)
                     /gene="der"
                     /locus_tag="VBC74_000499"
     CDS             complement(28282..29592)
                     /gene="der"
                     /locus_tag="VBC74_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEO6941473.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYT
                     TGEWLNRKFSLIDTGGIDDVDAPFMEQIKHQADIAMTEADVIVFVVSGKEGVTDADEY
                     VSRILYKTNKPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPLSSVHGIGTGDILDAIV
                     ENLPVEEENENPDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTNFV
                     DSQGQEYTMIDTAGMRKSGKVYENTEKYSVMRSMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHETGKGIIIVVNKWDTIEKDNHTVSQWEADIRDNFQFLSYAPIIFVSAETKQ
                     RLHKLPDMIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYLRFLENQIREAFVFEGTPINLIARKRK"
     gene            complement(29640..30542)
                     /gene="dnaI"
                     /locus_tag="VBC74_000500"
     CDS             complement(29640..30542)
                     /gene="dnaI"
                     /locus_tag="VBC74_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEO6941474.1"
                     /translation="MKSVGQALENQGRVPRNTNDELIQMILADAQVAEFIKTHQLSQR
                     EINISMSKFNQFLIERQKFKNKDSQYIAKGYEPILVMNEGYADVSYLETRELIEAQKK
                     QAISDRINLVNLPKSYRNIRMTDFDINNESRMKAMSQLLDFVETYPSYNHKGLYLYGD
                     MGVGKSYLMAAMARELSERKGVSTTLLHFPSFAIDVKNAISSGTVKDEIDAVKSVPIL
                     ILDDIGAEQATSWVRDEILQVILQHRMLEELPTFFTSNYSFNDLERKWANIKGSDETW
                     QAKRVMERVRYLAIEFHLEGPNRR"
     gene            complement(30539..31714)
                     /locus_tag="VBC74_000501"
     CDS             complement(30539..31714)
                     /locus_tag="VBC74_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEO6941475.1"
                     /translation="MSPIDEFTYIKQNKIVYDSNSLIQLYFPIMGSDAMALYDYFVHF
                     FDDGIRRHKFSEVLNHLQYGMPRFQDALVMLTALDLLTVYQATGTYLVKLNQAMSNEL
                     FLSNPIYRRLLEKRIGEVAVAELDMKIPKNARDISKKFTDVFSDLGQPKQEVNRSKNV
                     FDLESFKRLMMRDGLRFNNEKDDVLGIYSVSELYHLNWYDTYQLAKQTAINGMIAPQR
                     MKLQQNEGQHIKDNQSFTNNEKVILRESKNDSALVFLEKIKRSRKAVTTSGEKTLLED
                     LAKMNFLDEVINVMVLYTLNKTKSANLNKAYIMKVANDFAFQNVITAEDAVLKIRDFS
                     DQKVRTKTETKKKQSNVPEWSNPDYKDEVSPEKEIELEQFKTDALKRLERLGKDGES"
     gene            complement(31714..32193)
                     /gene="nrdR"
                     /locus_tag="VBC74_000502"
     CDS             complement(31714..32193)
                     /gene="nrdR"
                     /locus_tag="VBC74_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEO6941476.1"
                     /translation="MRCPKCGYNKSSVVDSRQAEEGTTIRRRRECEKCGNRFTTFERL
                     EELPLLVIKKDGTREQFSRDKILNGIIQSAQKRPVSSEDIENCILRIERKIRSEYEDE
                     VSSITIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITKRVRSKKSGSV"
     gene            complement(32342..33847)
                     /locus_tag="VBC74_000503"
     CDS             complement(32342..33847)
                     /locus_tag="VBC74_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEO6941477.1"
                     /translation="MKNKKDQFIGVKQPLSKKLSQLVFILFFSLFTVFSVLVYTSATR
                     YVLHREKINVGRSLEKTRVRLSQANSSLTSDDILEILYNQVFADDIYPHKRQNGIVRT
                     GESIDSILYVNQEMTLYDVNRKPVFSTLRTGMPTIGKSMGKVIISKVADMEGFVGTKA
                     IYSQKTGQLLGYVQIFYNLGRYYSMRQNIIVFLIMMEVLGTVLALVVINSATKRIVRP
                     VKNLHDLMHQISENPSNLEIRSKVRSEDEIGELSRIFDGMLDQLEDYTRRQSQFISDV
                     SHELRTPVAVVKGHIGLLQRWGKDDPEILEESLAAAYHEADRMSLMINDMLNMIRVQG
                     SLELHQDEVTDLSSSISVVIENFRILREDFQFIFENNISDIVWGKIYKIHFEQALMIL
                     IDNAIKYSPSYKEVSVVLSVDNDFATVVVKDKGEGISDEDIEFIFDRFYRTDKSRNRE
                     STQAGLGIGLSVFKQIMDAYHLKVDIKSELNQGTEFIVRIPIKKFEETKEI"
     gene            complement(33837..34526)
                     /locus_tag="VBC74_000504"
     CDS             complement(33837..34526)
                     /locus_tag="VBC74_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6941478.1"
                     /translation="MGKKILIIEDEKNLARFVSLELLHEGYDVVVETNGREGLDTALE
                     KDFDLILLDLMLPEMDGFEITRRLQAEKTTYIMMMTARDSVMDIVAGLDRGADDYIVK
                     PFAIEELLARVRAIFRRQEIETKTKEKGDSGSFRDLSLNTHNRSAMRGDEEISLTKRE
                     FDLLNVLMTNMNRVMTREELLEHVWKYDVAAETNVVDVYIRYLRGKIDIPGRESYIQT
                     VRGMGYVIREK"
     gene            complement(34761..35294)
                     /locus_tag="VBC74_000505"
     CDS             complement(34761..35294)
                     /locus_tag="VBC74_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEO6941479.1"
                     /translation="MLLTEIKKSPEGLYFDKKIDIKESLMERHSEIMDISDIQVSGHV
                     VYEDGLYLLDYNMAYDITLPSSRSMKPVVLSEKQTINEVFIEAENVSTKKELVDQELV
                     LILEEDDINLEESVIDNILLNIPLRVLAADEVGVEADLSGKNWSLMTEKQYEEKQAKE
                     KEKSNPFAALEGMFDSD"
     gene            complement(35399..36289)
                     /gene="htpX"
                     /locus_tag="VBC74_000506"
     CDS             complement(35399..36289)
                     /gene="htpX"
                     /locus_tag="VBC74_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEO6941480.1"
                     /translation="MLYQQIASNKRKTVVLLIVFFCLLAAIGAAVGYLVLGSYQFGLV
                     LALIIGVIYAVSMIFQSTNVVMSMNNAREVTEDEAPNYFHIVEDMAMIAQIPMPRVFI
                     VEDDSLNAFATGSKPENAAVAATTGLLAVMNREELEGVIGHEVSHIRNYDIRISTIAV
                     ALASAVTLISSIGSRMLFYGGGRRRDDDREDGGNILVLIFSILSLILAPLAASLVQLA
                     ISRQREYLADASSVELTRNPQGMISALEKLDRSEPMGHPVDDASAALYINDPTKKEGL
                     KSLFYTHPPIADRIERLRHM"
     gene            complement(36310..36864)
                     /locus_tag="VBC74_000507"
     CDS             complement(36310..36864)
                     /locus_tag="VBC74_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEO6941481.1"
                     /translation="MGTMILIAIIALFVIWLIVAYNSLVRSRMHTKESWSQIDVQLKR
                     RNDLIPNLIETVKGYAAYEGKTLEKIAELRAQVAKANTPAEAMTASNELTRQLSSILA
                     VAENYPDLKANNSFVKLQEELTNTENKISYSRQLYNTTTSNYNVKLETFPSNIVGKLF
                     GFKPSQFLETPEEEKEVPKVSFDF"
     gene            36992..>37261
                     /locus_tag="VBC74_000508"
     CDS             36992..>37261
                     /locus_tag="VBC74_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6941482.1"
                     /translation="MTPQAFYQVLIEHGITLTDKQKKQFETYFRLLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGYIDNSPLSILDIGAGAGFPSIPMKI"
BASE COUNT        11765 a         7371 c         5725 g        12400 t
ORIGIN      
        1 gggcggatag actggggtat taattagaac agcatctccc tcctttgtga aggcttgtat
       61 agcaactgaa atcgctggta caaccccatc aattaataat aatgattttt tatcaaattg
      121 ataaccatgt tcttgcactt cccaattaga gatagcttgg tataaactgt ctttaggact
      181 atcatagcca aaaaccattt ggtgtgaaaa atcttcaata gcctccgaca tttctggaaa
      241 gataggaaaa tccatatccg caatccaaag gggaaggatt tcttgatctt tttgaaccgc
      301 cttccatttt atggcattac ttgaaaaacg ttctggtaaa cttgtgaaat catatgtaat
      361 cataattact cctgactatt ctacagctaa aaatatccct tgtcgccttg gtgactattt
      421 taccatttgt ttttgatact atatactaat taacattcta aagataaaag tgaccatttt
      481 aaacgatcac tttctttatt attttaattt atcattttca taagtatggc ttaattctaa
      541 attaggaaag ctttgctgcc taagggcttc ataaacaatc atacaaacag tgtttgaaac
      601 attcaagcta cgcacatgct catcgttcat gggaattcgt aaggcctttt cggcatgctc
      661 gcgcataaaa gtttctggca atcctttatc ttcacgacca aacataaaat aatggtcttg
      721 atcatcattg taattttcat ctgagtaaac cttgtcagca aatttgctaa ttaggtgaac
      781 tttccctcga catgacaaca taaattcttc caaaccatca tagaaactaa catctagctt
      841 atcccagtaa tccagtcccg ctcgtttcat ttttttatca tcaataggaa atcccatcgg
      901 tcgtatgatg tgcaaaggag catttgttgc cgcacaagtt ctagcaatat taccagtatt
      961 tgctggaatc tgtggttcaa ataaaacaat atgatttcgt ccgctatcgg aacgatgatt
     1021 tttctgcgtt agtgtttcaa tattcatctc atcatcactt tctagaactc attctgactc
     1081 gcaaaaaaac cacactgctc ggagtcaatc tcagcaaaca atgtggttta tggtggtttg
     1141 ttaacttaaa tcataacagg tttgatttaa actaacaaat cgttgacact attatagcac
     1201 ttcagtctac aaagtcaacc tattcatgag tttttgggtt atttttttga tagatataat
     1261 gacaaatcaa acagaataat aagcaaaaac attggaaaat taaaaccgta aagaaattac
     1321 ttaacaaact cccgataagg aggatcaaac cgcctaaaat tgctagaatc ggtgaaacta
     1381 agccggtaaa tttattttta atatcttttt tgagataaag gctgagtact aaaacataaa
     1441 gaatgattaa actggtatta ttaaacacca cagcaatttc actaatatca ctacccggca
     1501 ataagttaaa ttttgtaacc ataaaatgaa caaaaagcca aaaaatagct acagccgtca
     1561 ccgtcaaact agctggcaat gacatttgat acttcaaatt tatgttagcc atcctctcac
     1621 ttttgatcca tccgcgttcc gcaaaagctt gaggtagacg catcgtccca agtaaaagtc
     1681 cattggcaac acctaataca gatagaataa caatgaaaga taataaacgc ccaacactag
     1741 gaccgaaaat aatattagct gcataattaa tagcatcatt accggttgtc ataataaagc
     1801 tagcacctaa aatttgtgtc aaaccataga aaaatgccaa atatgataat aagataagcg
     1861 ctggaccaat gacaaaggca agtggtaaat tcttcttggg attcttgact tctggtgcaa
     1921 tattaacaaa aattgtccaa ccatcatatg caaaatacaa tggtaccaga cctgacaccc
     1981 aactccatcc aacatttgac ggttgaatag ctgttaaatg ttgtggaagt tgtggagctt
     2041 tatcagacca aaaagcacca attaaagcaa ttagaactaa aggaatcatt tttacaaagg
     2101 tcgttaaaga ttggaatccc cctgcaatac gcttagcaaa gatattcatg agactaagcg
     2161 ctaaaatata aacagcagct aaaataatct gtgattccaa acttgatgat tcacctaaag
     2221 tataaaatgc agctacccat gaaacgattg ctgttaaagt tggaagatac aaaaaagacg
     2281 caaacaagcc taaagttgct gctaaagctg ggctaacata tttttcgtag taagaaaaaa
     2341 taccaccact ttcactcgtt ctaatagcta gctcagaaat tgacaaactt ccaaaaacaa
     2401 tcgaaaaaga acctagtact aaaattacca tgcctaaaaa gacatctcca ccagtaaatt
     2461 taaggatgtc atcaacttta aaataaatgc ctgaaccaat cactacacct acaatcatag
     2521 caattgtagt cattaatcca taggtctgtt taatagaatt atgattctcc ataatatctc
     2581 catttctact gtacgcttaa aggttaatta taacctaatt tagggcttgt cacaagttta
     2641 tctgtgttat ttttcaccag attctgataa gagaatcata ttactcttat cagaaagatg
     2701 aaaacgtttt ttaaccataa ttctcgtgca tttttactaa aaaaaaggta tcatagagtt
     2761 tattattgga ggtaaaaatg agaataattg ttgtcggcgg aggtaaagtc ggaaccgcac
     2821 tttgtcgctc acttgttgct gaaaagcacg atgttgtttt aattgagaaa aaagaaaacg
     2881 tccttaaacg tgtcactaaa cagcatgata ttatgggaat tgttgggaat ggagcaaact
     2941 ataaaatttt agaacaagcc gaagtcaaaa attgtgatat ttttatcgct atcactgatc
     3001 gagatgaagt taatatgatt tcagcagttc ttgccaaaaa aatgggggca aaagagactg
     3061 ttgttcgcat gcgtaatcct gagtattcta atccttactt caaagataaa aactttcttg
     3121 gcttctcttc agttgtcaac cctgaattgt tagcagcaca atatatcgct aataccatag
     3181 aatttccaaa tgcaacttca gttgaacact tcgccaacgg gcgtgtgatg ttaatggaat
     3241 ttaaaatttt agaaggaaat aaactctgtc atacttcaat gagtcaaatt cgtaaaaaat
     3301 ttggtaatat tgttatttgc gcaattgaac gcgatggcaa attgattatt cctgatggcg
     3361 atgcaactat ccaagtcaaa gataaaatct ttgtaactgg aaatcgtatt gaaatgatac
     3421 ttttccataa ttacgttaaa aataaagttg tcaaaaactt gatggttatc ggtgcaggac
     3481 gcatcgccta ctacctactc aatatcctta aaaataccaa tacacacgtc aaactagttg
     3541 aactcaacca agagcaagct gaatatttta gtcaagaatt tcccaatgtt cctgtcgttc
     3601 atggggatgg tactgctaaa aatattttgt tagaagaaag cgttacaaat tttgacgcag
     3661 ttgctacttt gactggtgtt gatgaagaaa atatcattac ctcaatgttc ttagaaagta
     3721 tcggtatccc taaaaacatt accaaagtta atcgaactag cctactagaa atcattgacg
     3781 ataaacaact atcaagcatt atcacaccca agcgtattgc cgtagaccat gtgatgcact
     3841 ttgtacgcgg gcgtgtaaat gctcaggatt ccaacctaga agctatgcat catattgcta
     3901 acgatcgcat tgaaacctta caatttgaaa taaaagaaac tagcaaatta gcaaatcgta
     3961 gccttgcatc tttaaaatta aagcaaaata ttttaattgc agcgattatt cgcaataata
     4021 aaacgatttt cccaactggt gaggatgttt taacagtggg tgatcgtatc gttgttatca
     4081 ccttattaaa aaatattact agaactagtg atatgttagc gaggtaggaa tatgaataaa
     4141 agtatgattc gtttccttct ctcaaaactt ttgctaattg aagctgctct tctagcgatt
     4201 cctctaactg ttggacttat ttaccgtgaa ccgcaaagtg tcatgatgag tatagtcatt
     4261 accatgatta ttttaatcat ccttggatta ctgggttctc tctttaaacc taaaaattat
     4321 catatctaca ctaaagaagg catgttaatt gttgcactct gctggatact ttggtccttc
     4381 tttggcgccc ttccatttgt tatttcaggg caaatcccta atattatcga tgctttcttt
     4441 gaagtatctt ctggttttac gacaacagga gcaactattc ttgatgacgt cagtgtctta
     4501 tcacccgcgc tcttattttg gcgtagcttt acccatctta tcggtggaat gggggtttta
     4561 gtatttgccc ttgccatcat ggaaaattct aaaaatagcc atttagaggt tatgagagct
     4621 gaagttcctg gacctgtatt tggtaaagtt gtctcgaaac ttaaaaaaac tgcacaaatt
     4681 ttgtatttgt tatatcttct tatgtttgca gtatttgcag taatattata ttttgcaggt
     4741 atgcctttct ttgatagtat tatcatagcg atgggaactg caggtacagg tggttttgca
     4801 gtttacaatg atagcattgc tcattataat agtcctctga taactaactt agtttctatt
     4861 ggtatgctta tcttcggggt taactttaat ctttactatc ttcttttact ccgaaaaatt
     4921 aaagcattct ttggtgatga ggaactaaaa acctatctcc gcatcgtggc aatagcaact
     4981 ttcatgattg ctctaaacgt catcggtatg tatgataatt ttcgacaagg cctcgaacat
     5041 attttctttg aagtatcagc tattattacg acaactggtt ttggcgtgac tgatatcact
     5101 cgttggcctt tgttttcaca ggttattttg ctattcttga tgtttatcgg tggttctgct
     5161 ggctctacag ctgggggctt taaagtcatg cgtagcttaa tcctagcaaa gattgcacgt
     5221 aaccaagtat tgtctacact ctatcctaat agagtcatgt cactacacat taataaaagc
     5281 gttctagata aaaatacaca acatggtgtt ttaaaatatc taactatcta tttagctatc
     5341 ttcatggcac tcgttcttgt tttaacgctt gatactaatg attttttggt tgttatcagt
     5401 gctgcagcct catgttttaa caacataggc ccactcttag gtagcaatga aactttctca
     5461 ttctttagtc cattctcaaa actccttctt tcatttgcta tgatagcagg acgtttagaa
     5521 atataccctg ttttattgat gtttattcct aaaacatggt caaaactata attacaaata
     5581 ataaaacgga tatatcaatc tgatatatcc gttttattac gtcttaagct aaaatgatca
     5641 gggacaggat catttcctcc gtgggctaag ggatgacatc gcaaaatacg tgcaatcccc
     5701 atcaacacac cttttagacc atgtttttga atagcttcta tcatatacgt agagcaagtt
     5761 ggacgataac gacagctagc tgggaaagct ggagaaatat ttttttggta aaagcgaact
     5821 aagaaaatca aaaaagattt aagcattaag atttattaat agccgcatta tgcaactgac
     5881 taatttcttt tttatttaat cgtcgtgctt ccccaggacg gagacccgtt aaatctaagg
     5941 taccgaattg tgtacgagat agcttgtcca ctaataaccc aacttgttca aacattttct
     6001 taacttgatg attacgacct tcatgaatag ttaattcaac tactgaacga tttttttcat
     6061 gatccacttt tatgatagta tagcgagcag gttttgtttt cttaccatcg ataacaacgc
     6121 cacgcgttaa tggtcgaagg ttttctttag tagctattcc tttaacacgt gctaaataaa
     6181 ctttatcaat ttcattacga ggatgaatca ttttatcagt aaaatcacca tcattagtca
     6241 aaattaaaag tccagttgta tcccaatcca agcgaccaac aggatatatc cgttctttga
     6301 cttgtggcaa caagtcaata actgtcttac gtcctttatc atctgagaca cttgaaataa
     6361 caccacgtgg tttatttaag agataatata ccttctcttc gttgtaaatt ggacttcctt
     6421 caatttcaac cagatcacca gcctttactt gggtcgccaa ttcattaacc acttgaccat
     6481 taatcgtgac cattccttgc ttgattaatt cctcagcttt tctacgactt gcaatccctg
     6541 catgcgcaat atatttatta attctcattg ttttccttat cttctgtatt aattgaatcc
     6601 attgaaaata acgatacttc ttcgtcaact aattcaattg aagaggcgtc aattaagtca
     6661 tctaactgat taattcccat ataatctaag aaataattag ttgtctcata aaggttagga
     6721 tgtcccaaca cttctttctt tccagcctct ttaattaaac caaaagctat cagtctcgtt
     6781 atagctccac tggaattcac gccacgaata tcatcaattt caatcctagt aataggttgt
     6841 ttataagcaa taatagatag aacttccaaa cttgcccgag atagactctg attaatcgga
     6901 gccttcgcat aatctctcaa caaagtcata taactatcct ttgttaccaa tttataagtt
     6961 tgtgctgtct caagcaataa tagactagaa tcgtcatctt cttcatatcg tttagccaac
     7021 ttttctagtt gctgtattaa tgctgatggt gtcaaagaca ataattctgc catttgacgc
     7081 aaactcagtc catcttcacc tgcaacaaaa agaagagctt ctattgagcc taaataagtc
     7141 atttctcttc ctttcttaag ataacttgac taaaattact gtcttgttct actgtgattt
     7201 gatgtaattt tatcaactct aacattgcca aaaataaagt tatcatttca gattttgttt
     7261 ggcaatccgc aaacacttct tgtaatgtcg ttttcttttt aagattaaaa tgtctttcaa
     7321 taacgatcat catatcttca atacggtagt cttctttctc aattaccgtt tgactatttg
     7381 acaattcttt ttgtttttgg gacatcattt gagaaaaagt caaaaaaaga tccatcaccg
     7441 atttatcgtg tagaagaatt gcatcttcaa agataacttc ttgttttggt tttgaaaaat
     7501 atttggcgcg ttcctgatgc tgattagcta actcttcact tagcacttta aaacgtctat
     7561 attcctctaa ttggctcaac aattccattt ctggatcatc ttcaatagga tttgactcca
     7621 ctactttagg taataagtta cggcttttta tcaacatcag ctgacttgcc ataagcatat
     7681 actcaccagc tacttctaga cgcatcgcct gtaatgttgc tatataagca aggtactgct
     7741 caataacttc cacaatcggt acatcgtata tatcaacctc atatttagat acgaggtgca
     7801 agagtaagtc taggggcccc tcgaaatctt ttaacttaat atccattatc tataatatct
     7861 ctctaaagtg ataggagttt ttaagcctag aaggcgactg agctccgtca tcgttttgcc
     7921 attttctttt tgctttagaa taaattgttc tcgcagaagt tgtgcagtta gttgttgctc
     7981 attcttctca tttaagaaat ctgttaattg attaaaaaac cattgtctag aataaggctt
     8041 accgttatgc tcaaagacta agtcaccatc ttcctctaaa gattccaaaa gaaaaggaag
     8101 caagtcttct ggaagtttta aaatacgctt catctgcgat ttttcaatac ttaagatatt
     8161 aaaagttgta tcaaaatcag ccttcttcaa attagcaatt tcagccggag ttaaacctaa
     8221 agataaaatc aacaaagcaa tcaaacgacc actcggataa tcagtatctt gatagaaatt
     8281 tgataaatcc attatttgag actttgtctg agccaaagtg attttttcag tctcttgtag
     8341 tctgtaaaat tccttcagcg ttcctctatt atataaaaag aaaagaaatt gattgactgc
     8401 agataacttt cgcttacgag ctgttaactt aaattcactt agagactgtt gatacaatgc
     8461 cagtttttca gaatttacat gtccaccaat tattttataa aattgcttta aatcatagtg
     8521 ataggactta cgcgaattga gagataattt cttactctca ataaaattat taatatcatt
     8581 tatcattgtt tttaggagtg atagtatatt cgtctgtaaa atcatgtagg aggctattaa
     8641 tagcttttaa aatcgattta cgggttataa tgcctctaaa atcattttga tcactgataa
     8701 caggtaaaaa tggataatct acaatcttat gcataattgc tgtcagagta gctgcctcat
     8761 taatcggctc gatttttgta ttaaccatct caacaatatc agtctgtgcc atctcccaat
     8821 ctgttaattg acctttagat tggtagccca taatgtctga gatactaata gtacctacat
     8881 attttttctc tttagtgata actggtacac gtgaaaaacc attacttgct aatagtaaca
     8941 taacgtggtc tgcattgtgt gtatccacaa aaatagcaac gtcttcagct ggaattagat
     9001 agtggtccaa atgatttagc aaaaacgact caaattcttt agcaatcatc ttgaaaattc
     9061 ctttgtcaag ttaggatagg gttggtgttg gcgagtataa tactctacca aaaccttact
     9121 atctgttata gttacaacag cgtaaagctt ctcatttatt ggtcctcttg gctgcaaaac
     9181 actacctgga ttgataaaaa tagttttacc atttttccaa gcatctgcac gatgaagatg
     9241 accatataaa caaatatccg cgtcttcttg ctgagctaat aaatccaatt tatcccaggt
     9301 aaaattaatc tgatggagat gaccatgggt ttgaacaata acagcattat ctatttttgt
     9361 aaccaaaact tccggataac ctgaatcata atcacaattt cctgtgacca catgaatgcc
     9421 ttcccatata ggatctgagc taggaagttc ggaatcccca ttgtgaaaaa tagcatccac
     9481 ttttcccaaa taatgattct ttatatcttt tacaatgtca cgatctccgt gagaatcact
     9541 cattacaact aattttctaa ttgccatttt ggaaatacct ccatcaattt acggacagct
     9601 tgcccacgat gtgatagatt atttttttct tgtgcgctca attcagctgc tgtcctacta
     9661 ccttctccga caataaagag cggatcataa ccaaagccat tttccccctt aggttccgta
     9721 ccaatataac ctggccactc tgcttcaact accaaacttt ctttatttgg agctgacacg
     9781 actaaggttg tatgaaactg tgctgaacgt ctttctttgt caaataccat ggctaattca
     9841 tgcaataatt tggcattatt acgagcatct gtcgcgtcag gacctgaaaa acgagcagac
     9901 catacccctg gcaaaccacc taaagcatct acttttaatc cggaatcatc tgaaattacc
     9961 atttttccag ttaattttga aatagtctct gctttaagtc gagcattttc ttcaaaggtc
    10021 ataccagtct cttcaacttc tggtaaatct gggtaatcat ttaaattttc aactttgagt
    10081 cctaatttcc caaataattc tctaaattcc ttcgtttttc cttcattgtg agtcgcaatt
    10141 aatatggtat ccccaaaatt attatcattc tttaaatcaa agaatttttc taaagaagtg
    10201 ccctctttag gtaagtgaac taagaacaac tgatcatctt cactaactaa gatggcccct
    10261 ttatgttgag tcactttgat atgccttgaa tactcttgat taatcatatc aacgaggaat
    10321 gctaataatt gaaaatccga agtcatctgc acaacgtgaa gctgtagacc ttcaactttc
    10381 aaagttgata atagacgatg aaaaacatct tggatagctt cgtaagattc atcattacca
    10441 aaacaagtaa aataattaaa agcttggaaa ctacctaaaa accaattgcc ttcagtttta
    10501 ctttcaaaaa ttgtttttgt cataatacga tacgctccac atttatttct tgactaagcc
    10561 attgttctgc aatttcttta aaaccttttg ggctggcggt tgtgtaaaag tgatgaccac
    10621 cgtgtttatt ttgccaatta tggtttatct caaaatagtt caataaaaca gaaatatcac
    10681 gaacggtttc tgcgccacta tcaattaatt taacctcagc ccccataaca ttttgaatga
    10741 tgggacgtaa taggggataa tgcgtgcaac ctaaaattaa agtatctaat ttaccaacta
    10801 atggggacaa cgtttcataa accacctttt tggctaaact agaagacatc tgatttgatt
    10861 ccacaattgg aacaaatttc ggacaagcaa gggataccac agcagtattt ggagacaaag
    10921 cttgaatttt ttgacgataa gcatctgatt taacagtcat gggagtacct ataataccaa
    10981 ctttccctaa attagttgat ttgatagctg cgctagctcc tggtaaaata acgcctaaaa
    11041 cagggatgtc tagtttttct ttaatttctt gccaggcaac tgcagttgct gtattacaag
    11101 ctataacaat catcttaaca tttttagtca ataagaagtt aaccatctgc caggtaaact
    11161 ctctaatctg ttgagcaggt ctaggaccat acggagctct agcctgatct ccaatgaaga
    11221 ttacttcctc ttctggaagt tgacggaaca tttccttaac aaccgttaaa ccacctacac
    11281 cagaatctag aaagccaata ggcctactat ccatttttat tcctccaata ttagtgacta
    11341 aaagaggctg gacaacagtc ctagcctctc tatacaataa ccaatcagtc aaatctacac
    11401 ttgaccaagt ttgcccaaat cttgtctaga aatctgatgg actatcactc actatttcat
    11461 cactaaacgc ttcaatgcta aaacactctt attttttagt tttgatagct gtttttgcgt
    11521 gttttacaat attacggtat gtttgttgaa ctttagcttc actaggtttt tgtcccattt
    11581 gactcatcat ttcacgaata gcatccggtg ttaaacgagg gtgctctcca atctcttttt
    11641 caatttgttt acgagcaata aaaatgcctc ccacgagacc accaaacaaa gcgacgataa
    11701 ttaataaaat ccaaatagtt gttgacatct attcattctc ctcatttatt tacatgactc
    11761 attatagcaa aaaaagaaag agtttacaaa tatatgtaaa caaaatcaaa gcctctatcg
    11821 tccttgtcac gtcaatacat tattaaattt tcactagagc atggttaagc acatagtcga
    11881 gtcctttatc ttatcctaag tgttttcccc aacttttgtg tcatccattt ccaaatgaag
    11941 agagctatgt aaaaatctac caatccatgt agcaaagagc ctagtcctat caaagaaaat
    12001 agtgaccata aataagttga actactgtta gaagtcattg tgattatata aacagtaata
    12061 aattcgagca gaccatgaat tatgttaata gctacagcaa ataacaatgt ttgccattta
    12121 ctcatcaata aagagggctt tgacttaatc aagaaagccg caattaatgc aaatccgata
    12181 tgtgataaag ctctaagaac gattacaatc ggaaaaccag ctagtagaaa tccaagtcct
    12241 gtccctaaag ctaccaatat tgcaactgga acagacataa aaatacttaa aaataaaggt
    12301 acatggcttg ctaaggtgaa agaagcaggc ccaataatta ttttagcagg catgattata
    12361 ggaatcatga ttgcaaaagc tactaaaata gaagtataag aaaccatttg tgttgttttt
    12421 tttgacattt ttatctcctt tgaagctatt atacaaaata accttacagg tgtaaagaca
    12481 cctgtaaggt tatttgtcta tgtataaaat tcctgctttt tttagagcct cacatatttg
    12541 gtcaaaaact gactgatctt gacaagaaat catatgtgta tgtagaccat ctgtcaaaga
    12601 ggatagcaat tctgcattgg aaacttttaa tttagcagta aaattatcaa tgtctttttg
    12661 gctttgaatg tttaagggag cagtcagcat cccataaata gggtgctcta cctccacatt
    12721 cataatgatt ccttgatacc gcaaaataat ttccagttct tcttctgtct gttctatgcc
    12781 atgctgacaa accaatctag ctgtgaattg atgagtactt agagcactgg acataaggta
    12841 tccttttggg gtagaaataa tatcacattg ttgtgcacgc aataaggcaa tatctccaac
    12901 aatcacctgc ctactaacac taaaaatctt tgctaatgtg cttgccgata tagcttcttt
    12961 tgttcctttt aaagtcgtta aaatattttc tcgacgctcc tgtgccttca tataacaaat
    13021 tccttctttg gtgttatttc acaattaaaa actggctaga atatctaacc agttagcctc
    13081 aatcatttcg tgcaaaaaga cgaagaatat ttaagaatag attaataaaa tcaagataaa
    13141 ggcttagtgc catcgaaact gcccaaccat ctgcgacttg gccgccttgg ctattgtaga
    13201 catatttaat catttggtta tcgtaagcaa tcaatccaga aaagatgatg acacatacga
    13261 tactcatgat ataactcata cccccactac cgataaataa attaacaaga cttgcaatta
    13321 aaatcccaat taaagcagcc attaacgctt tacgcatacc tgataaatct tttttagtct
    13381 tagctccaat caaagccatt gcaaagaaaa cagccgcaga tgttataaaa gcttgaagaa
    13441 ctgtagtttg cgtgtacctt gcgataatga aacttaaagt aaacccattc aaagctgaat
    13501 aaatcaaaaa taatggtaat gcagctggtg tgtttcgacg cgccgctcca ctagcaacaa
    13561 agactaaaac caattctaag atgattgctc caaaatacaa ccctggcatt ttttgcatga
    13621 tggcaatcat attttgtgga aacatataca gcataatcgc agaaacagca gctgacaaac
    13681 ctactcctat ccctacaaga ccgtaaatct tagcaaaaaa ttgattcagc cctgaatcag
    13741 attgtgtgta aatgacgtta tcattcatat gtattttcct attctaaatt ttaatgatta
    13801 tgactaccta agaatttgcg actaaaacgg ataggtttct taaaaatacg tttgagtgga
    13861 cgagaagcct ctcgtactgc ccaatactta tcaaccatag tcacaatata actttcttta
    13921 taacgtggcg gtgcaatagt cgctccccac atgtgcttca aaataatatc ttcttctcta
    13981 gcgtttaaat cagttaattt tcgcgcatta cgaaccgcta tccttggatg aacccaggca
    14041 tggctcttgt taaatttcgt tacacgccaa tcataataaa agaaatcatg tagtaaacca
    14101 cctcgagcag tactactcgc atcccaacca aacttcttag caattttata acttgtataa
    14161 ctaacattta tagaatgctc taagcgagtt gagtgatgat gttggacaat agcttcgagt
    14221 ttttggaaac gtggatggtc aataaggtga ccgaccaaat ccatgaagtc cttatcgtct
    14281 tgatactttt tcattataat cacccctctg gggatattat aacatatgaa cttaaattaa
    14341 gctgaaaatg actactcccg cagctactgc tacattaaga ctttctgctt gtccaggcat
    14401 ttcaatatgt gctaaaacat ctgcttcttc tgtcatagtt ttggaaattc cctgtccctc
    14461 atttcccatg attaaagcaa aataatcata tttagggaga ttcttataat ttaccgattg
    14521 ttcagataaa gttgtcgcaa tcatagttat cccttctgct ttacaaaagt taacaaactg
    14581 ctctatttct accctaaata ctggtaaatg aaagtgactg ccttgcatag aacgaagtgt
    14641 tttttggtta tacaaatcag cagatttctg agataaaaaa acagcatcaa aattcgctgc
    14701 atctgcagtt cgaaccattg ttcctaggtt tcctggatcc tgcacatctt ctaatacaag
    14761 aaaacgtcct tttttaatat ctgtccatct tgtctcttga aaggcaatct cagctacaat
    14821 cccttgagga gtctgtgagt cagttaattc tttcaaaaca tcatctgtaa caacaatagc
    14881 tctctcgggt tttctcaaac gatctattgc cgtttctgtc acaaaaatat ttaaaaattg
    14941 tgcaccatat ttttcagctt cctcaaataa atgccagcct tcaataagat atgaagattt
    15001 ccgatatttt ttttgtaaca actttttcgt ttttttaatg agattatttg attttgaagt
    15061 tataatagta gtcataaacc tattatatca gaaaagaggt caagttatga aaaaagttca
    15121 tttaattgtc tcagggcgcg ttcaaggagt cggatttcgc tatgcaactt attccttagc
    15181 tttagaaata ggtgatattt acggtcgcgt ttggaataac gatgatggaa ctgttgaaat
    15241 tttagcacaa tccacagatt caaataaaat gacacaattt attcagaaaa ttcgaaaagg
    15301 accttcaaaa tggtctaaag taacttatgt tgatattaaa ctagataact tcgatgattt
    15361 taatgacttt aaaatgagta attaaaaatc attctgcgga agtattgttt aattcttaaa
    15421 aaaaaagtaa aatagatagg tatattaaaa attagaggaa ctaaaaacat tgaaaaaaac
    15481 attgaaacgt atcttattct ctagcttatc tttgtctatg ttattattat tgacaggatg
    15541 tgttagcgtt gataaggctg gaaaacccta tggagttatc tggaatactc ttggagtgcc
    15601 tatggccaac ctcattactt atttcgctca acaccaaggg cttggttttg gtgtcgctat
    15661 cattattgta accgtcatcg tacgtgtcgt tatcttacca cttggacttt atcagtcttg
    15721 gaaagctagc tatcaagctg aaaaaatggc gtactttaag cctttatttg aacctattaa
    15781 tgaacgacta cgcaatgcta aaacacaaga agaaaaatta gcagctcaga cagaattaat
    15841 gacagctcaa cgtgagaatg gccttagcat gtttggcggt attggctgct tgccattact
    15901 cattcagatg ccatttttct cagctatttt ctttgctgca cgctatactc ctggagtctc
    15961 tagtgcaacc ttcttgggac ttaacttagg tcaaaagagt ctgatactta ctgtcattat
    16021 cgccattctt tattttgtac aatcttggtt gtcaatgcaa ggtgttccag atgaacaacg
    16081 tcagcaaatg aaaacaatga tgtatctgat gccaataatg atggtattca tgtctattag
    16141 tttacctgct agtgttgcgc tttattggtt tattggtggt attttcagta ttatccagca
    16201 gctagttacg acttatgtac ttaagcctaa actacgccga aaagtagaag aagaatatac
    16261 taaaaatcct cctaaagcct acaaagctaa taacgcacga aaagatgtta caaattcaac
    16321 taaagccact gaatctaatc aagcaattat tacatctaaa aaaacaaatc gtaacgccgg
    16381 aaaacaaaaa agacggggat aaaacaagca tactaaataa tggtgtgttg tcctaaacag
    16441 ttaaagatta ttatgtaatt caagtaaaaa agacttagtt ctatgtccta catagctaag
    16501 tctttttagt tttgcctaac ttttcatata aaaagcaccc taaaagtcag ataaaaatct
    16561 agcttttaaa gtgcaagtaa tctagctaac agactacctt attaataaag taatcgtttt
    16621 tcccaccagt cttgaataat tgcataaagc gtacaaatta cccaatagat aagggctact
    16681 aagatatagg cggtcatata gtcggagtag gttcccccaa ctattttagc attattaaag
    16741 atatcaggta ccgtaatcat cgcagtcaat gaggaaccct tgaccatatc tagcaaaaca
    16801 ttacttaaag aagggagtgc aattcttgtt gcttgcggaa taataatgcc tctataaatc
    16861 gtcggtgttt ttaaccctaa agctctagca gcttcccact gaccatgatc aacagctaaa
    16921 agagctgctc tgataatttc tgatatatat gcactagaca tcatggtaaa acctacgata
    16981 gaagcagtca cagcatctaa ctgtatccct ataaaaggca aaccaaagta gataagaaag
    17041 agtagaacca ttaaaggaat cccacgcatt aaggaaatat gaatattagc tagatacctc
    17101 agtaagcgga aatgagacat cttcattaga gctacaaaga atcctacgat agctcctaaa
    17161 ataaagccaa ccaaggataa tccaatggta tatggtaacc cctgaataac acttggaaaa
    17221 gccttaacag ctagttccaa attaaaaatg gcatcccagt ttatcactta tctactcctc
    17281 tcgaaatttt ttatttaact ttaattactg gaattttatc cgttccagca ggttcttttg
    17341 tgagatcttg accagcataa tatttttcag agattttttt caaagaaccg tctttcttca
    17401 tatccttgat agctgcatca atttttgttt tgaggctctt atctttttta ctcatcacaa
    17461 taccttgctc agtcggatta tacttcacat cacccatttt tactttgata tctggatatt
    17521 gttttgttac atattttaca gcaatgactt gtgtgtagta atcattcgga atgaaatctg
    17581 tacgtccagt tgatacatct cgaaggtaaa cgtcattagt tacattgtca taaataacag
    17641 gttcagcacc ttgctgtttt gcaattttca tatattgcgt tccagcgcca ccaccagctt
    17701 ttttaccttt ccagtcagat aaatcttttg ctgtaatctt tgaagaacca tctgcacgta
    17761 caatcatacc tccaacagaa tatttataag gttgagagaa attgtatttt ttcaaacgct
    17821 cgggagttgt gtcaaagtta ttgacagcaa catccacctt accactatct acagaagtaa
    17881 atgattcagc aactccaact tcaacaaact taacttttat cttaagtttt ttagcaatcg
    17941 ctttcatcat atcaatctca taaccagtca attttttatg gtcatcataa taacttgttg
    18001 gataaagcgt tgcaggggta gcaaccttaa ggacaccatc ttttttaata ttgtcccatg
    18061 ttgtttcttt gttagattga ccatttgagc aagctgctaa tgtcattgca gataaaccga
    18121 ctaaaccaag taaaattgtc tttttcataa gtactcctgt agattaaatt atattaaaat
    18181 ggtttggcgt actctatcga aaattaaatt taatgaaaat taatacataa atttacgata
    18241 ataatacaac cattgttaag agtaccatat tgtatccgtt ttcgcaaaaa tattgtttac
    18301 ttttcaaatt aatggtaaac aggccctttg aattgcttct ctttttcaaa caactctgca
    18361 acagcaagca ataaatcttc tctcccttta gccgctgtca attgtacacc taaaggtagc
    18421 ccctctttag ttagatgagt tggtatggct aaagaaggtt gccctgtcaa gtttggctga
    18481 tgtgtaaaag gtgtccaggc cattgagtct tcaaacattt tccaaattaa atcctgttgt
    18541 tcacttactg agaattctcc catatgcctc aattgtttct ggagtgtctc gtctaaatca
    18601 aattgtccat gaaaaggagc tggttgatta gttgctgctg ttaaaattaa atcataattt
    18661 tcatgaaacc tcgccatggt agctgcaaat tgatcccatg aatctaagag tttactgtaa
    18721 tctttagcca ataccctttg tccactctga tacatagccc aggtcattaa ttccatatcg
    18781 ctaaattcca ttgaacgccc taaactcttt tcgatgtcat caaacattgc tgctgtttca
    18841 acgctattca tcatacaata agttttcata gaatgaatac catctaaagg ttgttctact
    18901 aattctaaat gattcccttt ttgagacaga aaatctgcgg cttctttaat agctgcttta
    18961 gcctcacttg atacctttgt ttttaaggga gaatccatca atactgctat ttttaggggt
    19021 ttactaacgc taaactcgaa aagactctct ttgctgagtt ttttcagggg aaatgggctt
    19081 tctacttgat atgattgtaa ataatataat agtcgttttg tatctctaac tgactttgtt
    19141 aaagcaaaat gactagaagc cccttgccaa cctctatagg atgaaggtcc aactggtata
    19201 cgtcctcgac tcggttttaa accaatcaaa ccattaaaac ttgctggaat tctgattgac
    19261 cctcctccat cactagctcc tgcaattgga accattcctg aactaacagc tgcggctgca
    19321 ccaccactag agcctccagc atttcttgaa tggtcaaatg gaagattaac attcccgtga
    19381 agttgaccat cactaatatt cttaaaacca aattcagggg tatttgttct tcctaaaata
    19441 ataaacccta atttttcaaa ggactgcact aaatagtccg tttgtttagc atgatagtgt
    19501 ttaaataatt gtgaacctga ggttgagagt tgtccttttt gattctgtcc caaatctttc
    19561 agaagaatag gaactcctgc aaaaggagca tttgattcat ttgcgtattt ggctgcttga
    19621 cgcgcctcat tatattgctt gctgacaaca gcattaacgc ttacattctg ttcctcgatc
    19681 ttgtagattg cttgctctac taattcttgc gacgaaatct tatgctgttt tatcgcttgt
    19741 accatagcgg tagcatcttt aaaaaccata taaacacctc ataaaatgta tttttattat
    19801 aagataacaa aaaatactta aaaaaaatag aataacgtct agactctaga tattattcta
    19861 tgatttatac tattaatttg ttttttcaac actaatgatt tcaacttgat agctaccagc
    19921 aggtgattca attgtagcta agtctcctgt ttttttacca attaaagcat gagcaattgg
    19981 gctttcattc gaaatttttc ctgaaaaaat gtcagctcca gcagcaccaa cgatatggta
    20041 agtatcttta tcatttgtac caacttcttg gacaaggaca gttttaccaa ttgcaacctc
    20101 atctttagca actgcatcac tatcaataat ttcagcataa cgaatttttg tttctaaaat
    20161 ttgaatttga ccctcaacaa atgcttgttc gtcttttgct gcatcatatt cgctattttc
    20221 tgataagtca ccataagaac gagcaatctt gatacgctct acaacttctg gtctacgaac
    20281 taattttaac tcttcaagtt ctttttcaag ttgatctttt tccacttgtg tcattgggta
    20341 agttttttct gccattttat ttctcttttc ttttcatttt ttcaagtcaa agatataaac
    20401 atgatgactc gcaccatgtt tataagctta gttaatttga ctattgatat atttttcaac
    20461 gttagccttg tgtgtttcaa aatctttagc gtagtataca tttccggtct tcacatcagc
    20521 gacaaaataa aggtaatctg ttgaagcagg ttttatagtc gcttcaattg ctgagatact
    20581 tggactatca actggtccag gcatcaaacc agtattagta tagatgttgt atggtgattt
    20641 aattgaagta ttaatttgag catcttcagc taaacttgtt ttatcaccta atttccccat
    20701 agcatacaaa attgcaatgt tactttgaag agcttgaccg gctgataatc ggttataaaa
    20761 gacacttgct attttacggc gatcttcgtc tgttgatccc tctttttcaa caagagatga
    20821 taatgtcaaa acatcattta cagacatatt cttcgcttta atagtgttat agtaaggtgc
    20881 tatcttagta ttcattgttg aaatcatatc ttcaacaagt ccttctaagg ttgtatcttt
    20941 ataatagttg taggtagctg ggaataaata tccttccaat tgataaatag cttttgattt
    21001 atcaggtaaa cttcctagta aatttggata tttagctacc atttttttaa taaacgtttc
    21061 atcttgaaca agtttcaaga aatcatcagc tttataaggt gtacttgtag tctttgtatc
    21121 aattttatta gactcaatag ctttagcaat ctgtttaata gtgtaaccct cggtaattaa
    21181 tatcttacct aaagctggtt ttgtcggttc tgcagttccg cctttttcca attcagcagc
    21241 aatctgatct aaagtcatac tttttttaag attgtagtat ccactttgaa aattactata
    21301 gtttttgaac ttggaatagt aattaaagac tgtggcgctc ttaatgactc ctgctttttc
    21361 taaaatttga ccaattaatt tattcccaga tcctatcgga atttcaactt gtacaaattt
    21421 atttgaattg gaatcaacag gtttaatagc actatctaca aaacgataac ctacaaatgc
    21481 tgtcaagagc aatacaagta tgataattga tatcagaata cgtgcaattc gttttgccac
    21541 attatctgct ttctgacgtc tttcacgctt gttatgtttg ttcttggctg acaaagctat
    21601 accctcttct tcttttgaca aatcctctac tcggataggt tcctttgcta cgggctctaa
    21661 ctctgatgtc tcaccaagtt ttacattttc accttctgta ggaatatctg ttgctctcac
    21721 aagaaagatt tcttccgttt cttgattagg cacagcagtt gcaacatcag tcttatctgc
    21781 ttttaaatct atgtcaggta cattttcagt tgtcctaaat tcactagatt tggagccgct
    21841 atcaagctct cttgaaggtt ccatcccttg attgataact tgttcttctt gggaagctaa
    21901 tgcttgttcc gcctgacgtc tttcttcttc tcgagcagct tctgccagaa gtttcgcctc
    21961 aatccgagcc ttctcttgct cttctaagcg ttgcttagtc tctagcgcct ttgctctagc
    22021 ttcttgttcg tctttcaaac gttgcgcttc ataatctgcc atcaattgtg ctgttctgcg
    22081 agcagcttct ttggcttctt gctctttttg atagagttct tcttcacgca actttctcaa
    22141 acggttagct tcttctaact ctgccagaat ttgttcttta aaacttttct gatcatggtt
    22201 agaatgctga tcgtcattaa attcggtcaa agagatttcc tcctaataat aatcacaggc
    22261 aattatatct taaatagtct ataaaatcaa caaaactttg acaatgttat ctacttttaa
    22321 caaattgatc gtgttggatt attccaaatc atattatacc aaagcgaacc accatgttgg
    22381 gagtcagatt ctccctcatc tttaaatcca ttcatttcat agaaggaaat taaatcatca
    22441 tggcaagtta aacttatacc atctcgttct tgagaaacaa ccaggtcttt catcgctgct
    22501 aataatgctg taccaatccc ctgaccttta aaatcaggat gtattgatag actagtaatt
    22561 cctatgaaac cacctaccct tacagggaat tcagaaactt tatggaataa atcgtccgtt
    22621 aagtaccgac ctttaataac cggtccttca atgtaacctg ctaaacgacc attaatctct
    22681 gcaactaaaa aagtatcagt cattatcgtt aaacgttctt gcattgcttt tttactcgca
    22741 gcctctgctt ctgaaaaatt tatttgttca atactaatga cttgctgtaa atcctctagg
    22801 caaccatttc taataatcat actcatctcc ttaatttctt tatgaaattc tcttgataaa
    22861 gtcagataga ggactgagaa acttctctca gcccttacca tcaacttatt gtgtattttt
    22921 agttaggtta gctaataatt cttcaaaaga gcgctcatac aattgaatgt ctccagcacc
    22981 cataaagaca tagacagcat tatcatgatt gagtaaagga gagacatttt cgactgtcac
    23041 taaatctgag tgtttgacaa tcttagcagc taaatcttct accttcacct caccattatc
    23101 tacttctcta gcagaaccat atatttgagc gagataaacg ctatccgctt gactcaaggc
    23161 atgggcaaat tcgtctaaaa gagctatcgt acgagtgaac gtatgcggtt ggaaaatagc
    23221 tacaatttct tttgacgggt atttttgtcg agcagcatct aatgtcgcaa taatctcagt
    23281 aggatggtga gcaaagtcat caataatgac agtatcgtca ataatcttct cagtaaaacg
    23341 acgctttacc cctgaaaatg tcttcaaatg ctcagctact aatgccatat caattcccat
    23401 tatgtaaagg ttagcaataa cagcagttgc atttaagata ttatgtttac cgtatgctgg
    23461 tacatgaaac tgaccaattt cttcttggtt atagaaaacc ttaaagtcag aaccattaac
    23521 agttcgagtg atgtcttttg ctataaaatc atttgaatct tcaaaaccat aataatatat
    23581 tggtgcctca gaagtgattt catgaagttt tggatcttct ccataaatga ataaaccttt
    23641 ttgaacttgc ttagcatagt cattaaaggc attgaatacg tcctctaggc ctgtaaaata
    23701 atcaggatgg tcaaaatcaa tattggtaat aattgagtat tctggatggt acggcataaa
    23761 atgacgttcg tattcatcag cttcaaacac aaagtaatta gcattagcag aaccacgtcc
    23821 tgtaccatct ccaattagga aagaagtgtc tgtaatattt tttaaaacat gagctaataa
    23881 acctgtcgtt gaggtttttc catgtgcccc agctacacct agactagtga actgacgcat
    23941 aaaatctccg agaaattcat gatatcgttt aaaatgatag cccttttcaa taacataagc
    24001 caactcttca ttgttatctg gacgaaaagc atttcctgca ataatctcta aatcctcact
    24061 gatattattc ggtgagaaag gtaatatagt tacacctgct tgctctaaac cacgttgggt
    24121 aaaataatat ttgtcaacgt cacttccttg gacgttatgt cccatttgat gaagcatcag
    24181 tgctagggca ctcattccgg atcctttaat accaataaaa tgataagttt ttgacatggt
    24241 ctctccttaa ttaatgtctt taaaacgtgt cagatttaat tcttgagcct tggctctttc
    24301 tttatgaaac tgattgtctt gtttattgta aacttgactg cgtttcaaaa aatcaaaatt
    24361 atttttcttt gtttttgggt tcggtgtatt atcaggttct tgaaatatga ttggaatttc
    24421 tgctaatata tagctttcct gctgtaaacg atcagaaaga tgacttagtt cagtcacctt
    24481 tcgctgagtt tgttttttcg gtactgttgc cttcaattgt tgctgaaaag cttcctttga
    24541 tggcgcctta aattcctgtt tcgcatagta agcttgtcgt ttctcccgaa cagacctctt
    24601 tgcctcttgt cgagatttgc tcaactctgt cgcttttttt acaaaactct gcggtggtac
    24661 aggatcattt tcaattacct taggatgtga tatttcttca tgttcataat tgcttttgac
    24721 catgctttga tatgtttttt cttgataaaa gcctcgaatg ttgttaatta aatcttcatt
    24781 atcataaaga ttcatcttta caagcggaga cattactggt tcatcatctg ctatcaacgg
    24841 aaatcgtcta gagcctctat tcacattatc acttcctttc tacaacagac ctattataaa
    24901 ctaatttttc tgttttttca aagttttcaa ctaagtaaag cttatcagat tggtattttt
    24961 cattcattta tataaaaaag aaagcgagag accactttct tttttatatt atagtcacat
    25021 catcatattt atttagaaac tccaagtatc tctcgaatat catctacgct catgctagca
    25081 tgggtttcat ttccatccag aactgttgta accaaatgtt ttttagtttc ttgcatttct
    25141 agaatttttt cttcgattgt gcctctagta atcaagcgat aaacctcgac attctctttt
    25201 tgccccaacc tatgtgcgcg actaatcgcc tgcatttcaa cagctggatt ccaccacaag
    25261 tcaatcagaa caacggtatc agctcctgta aggttgagac caacgccacc agcttttaaa
    25321 gaaatcaaga aagcatcttt tgatcctgca ttaaatgctc tggtcatttc atgacgttca
    25381 tttgctggtg tagaaccagt aattttataa gttgtaagcc ccatagctac catctcacgt
    25441 tctgcgatat ctaacatacc cctgaactga gaaaaaatca gagcacgatg tccattttcc
    25501 ttaatttgtg ttaaaagttg acgtaggctt tctaacttac cgctttcacc atcataatcc
    25561 ataaaaagac gtggagtgtc acaaatttga cgtaatcgtg tgatgccaga taaaatttca
    25621 attttacgac gatttaagtc agcgtcacta gaatgttgga tactttcttg aatttggcgt
    25681 aattgcgcca aatagataac tttttgactg tctgtcattt catttggata gttcatttca
    25741 atcaaatccg gtaattctgg gagaacttct tcttttcgac gtctcatcac aaatggtttg
    25801 atataacgag caacttgttt agggttcaac ttcaaaaact ctttcttacc aggtaataaa
    25861 ccaggtaaaa tgatctggaa aatggaccaa atttctaaca gtttattctc aataggtgtt
    25921 cccgataatg caaaacagtt tttgacctca aaagatctca agctatgggc tattttagtt
    25981 tgagcatttt tcataacctg tgcttcatcc aaaattaaat aatcataatt tccttcagaa
    26041 taagtctcaa aatcctgcct aaatgatgaa taacttgtaa tgattacttg atgacgttca
    26101 gcaataattt catctctacg aatcttagaa ccataggcaa ctgctacatc tacgtctggc
    26161 gcaaatttgt ggaattcatc ttgccaatta taaattaaac ttgatggtga taaaataagc
    26221 acacgagaat ctctagttag ttttgtactt aaaaatgaaa tcgtttgaag tgtttttcca
    26281 agccccatat catcagctaa aatccctgca aagccataat gatctaacat acttaaccaa
    26341 cggacacctg taagctgata atcgcgcaag gaagctgaaa cagatagacc aggtatttta
    26401 aacttttctg ggtgtctcaa atcttcaatc aattggtaaa aatgttgtga aaagctaaca
    26461 ttatcttgat ttgcaaacag ttctgataat tgaaatgctg caatattatc taattcaata
    26521 tgcccattct ttgctcgtct agctctcaac ccttgcaaag ttgcacttac tttcttagtt
    26581 tcttcatcaa aaataactaa ttgccctgat ttattcacaa aataaggatt attgtcaaac
    26641 aaagctacca gagcttgatc aacatcgttt tccagtaaat cagagaaatc aaaagaaata
    26701 tctagcaggc ttcccttagc ttcaatatca atttgcggtc gttctatgag acgataactt
    26761 tccaattttg cggataagct cactgttccc atttttttaa attgtggaag catataggta
    26821 taaaaatcgt ataattcttg tgcactgtat gctggatggc tggttgaaaa gtggggtgca
    26881 aagccatatt tttctaagag tttaaatact ttttcttcat gtttgaagtg actagcaaat
    26941 gttaactgtt ctagttcttg acgattatga acagttaaat cattaccata atcaaatacc
    27001 atttgcaata aaatttcatt ttgactatta atatcgaact caaaatttac cttaaaatca
    27061 tgaatagaga agctccgtgg agcatccact agtccaatct ccttaaaatc cagtaaatga
    27121 gctgctaact tatcttgatc atctaaatca aaatggatat gttttgctaa atttccctca
    27181 atgggaagac tacgaatagc cgttaccatc ttttcttgct tcaaattaag atggtagaaa
    27241 gtatcgcgat aaaacaaata agaattcgca aatagtggtt ttaagtcttt ctctttgatt
    27301 tctaattcaa ttgattgcct atggacaata accttaaatt gataaagatg atcctcacct
    27361 tctaattcct taaagtagag gtgattaaat tgatgaaaat cactttcaaa tgaaaaattt
    27421 tctaaagcat tcatcaaagt aacaccctct tcaaaaaaac cacgagtgag actaaggtga
    27481 cgaccttgat ttggtaaaat aaactctaga tcaattttgc ttgaatgact tggaatgagc
    27541 ctccataaaa attctatcaa ttcttgactg gtctcatcaa actgtatcaa agacaaagtt
    27601 tcaaagtatt gttttccaat ctgataataa gcttcttttc gaacagtatt taaaaaggct
    27661 ttgatgtcac gaataacata acttctatca tcaggcagtc gtctaatttt taaagtccac
    27721 cagatatcat tagcataagg attttgttct ccttgagccg ataattgata ttttatagta
    27781 tcatcttcat taataattag actatctaaa aaaacactac caaaagaggt tttcttctta
    27841 gtttcttgtt gactttcttg ttgaacttga actttagata agattgcttt accttctgga
    27901 tcgtttttca agaaatgctc cagagcagct aaatgttgac aatagccttt tcttgcaaaa
    27961 aaatcacaag aacattttgt ttcttctgtt actaatgaat attcaatctg acaatcccca
    28021 actttagctt ttaatagatt accttcttga gaaatcaagg acaccaaacc ttgttcgtag
    28081 agttctatac cttgatttcg aatacgtcct ggaatcattc tactcattat gctaccacct
    28141 tcatcttatc ccttaattat aacaaaattc aagtccaatt tcttgtaaaa atactaaatt
    28201 gattgttttt tgatacagta acaaataaaa aaggctaaaa caactgtcct aaccttctat
    28261 ttcaatttca acaataagat gttatttacg tttacgtgct attagattaa ttggtgttcc
    28321 ttcaaaaaca aatgcttcac ggatttgatt ttctaagaaa cgaagatatg aaaagtgcat
    28381 caattcttct tcgttaacaa aaacaacgaa agttggtggt ttaacagcaa cttgagttgc
    28441 gtagaaaatc ttaagtcgtt ttcctttatc agttggtgtt ggattaatag caatagcatc
    28501 cataatcaca tcatttaata cagcagacgg aatacgtttg ttttgacttt cgctaatacg
    28561 tttaatcata tctggtaact tgtgcaaacg ttgctttgtc tctgcagaaa caaagataat
    28621 tggcgcataa gacaagaatt ggaagttatc acgtatatca gcttcccact gactgacagt
    28681 atgattgtct ttttcgatag tatcccattt attaactaca ataataatac ctttaccagt
    28741 ttcatgtgca aaccctgcaa tacgcttgtc atactcacga ataccttcct ctgcattaat
    28801 caccataaga acaacatctg agcggtcgat ggcgcgcata gaacgcataa cagaatattt
    28861 ctctgtgttt tcatatacct taccagattt acgcatacca gcagtatcaa tcatagtata
    28921 ctcttgcccc tggctatcaa cgaaattagt atcaatagca tcgcgcgtag taccagcaac
    28981 tgggctagca ataacacggt cttccccaag gatggcatta attaaactag atttcccaac
    29041 atttggacgt ccaatcaaac taaaacgaat aatatctgga ttttcatttt cctcttctac
    29101 cggaagattt tccacaatag catccaatat atcaccagta ccaataccat gaactgatga
    29161 caagggataa ggatccccta aaccaagaga gtagaaatcg taaatatcat tgcgcatctc
    29221 aggattatct accttattaa cagctaaaat aaccggttta ttggtcttat aaagaatacg
    29281 tgagacatac tcatctgcat cagtcacacc ttctttaccc gacacaacaa acacaataac
    29341 atcagcctct gtcatagcaa tgtctgcttg gtgcttaatt tgctccataa acggtgcatc
    29401 aacatcatca atcccaccag tatcaattaa tgaaaactta cgatttagcc actcacccgt
    29461 tgtatagata cgatcacgag tgactccttc aacgtcctca acaatggaaa tacgttctcc
    29521 tgcaattctg ttaaataatg ttgattttcc aacattcgga cgaccaacaa tagcaactgt
    29581 aggtaatacc ataattctcc tctcactatt gggaatactc ccaattccat tctattttat
    29641 taacggcgat tcggtccttc taagtggaat tctatcgcta aataccgtac gcgctccata
    29701 actcgttttg cttgccaagt ttcgtcgctt ccttttatat tagcccattt tctttccaaa
    29761 tcgttaaaac tataatttga cgtaaagaaa gtgggcaact cttctagcat acgatgttgt
    29821 aatataactt gtaaaatctc gtcacgaacc caggacgttg cctgttcagc cccaatatca
    29881 tctaaaatca aaatcggaac acttttcaca gcatcaattt catcttttac agttccgctt
    29941 gaaatagcat ttttgacatc aatagcaaaa ctagggaaat gaagaagagt cgttgacaca
    30001 ccctttctct ctgataattc cctcgccatt gctgccatta aataggattt cccaaccccc
    30061 atatctccat ataaataaag ccccttgtga ttatatgaag gataagtctc tacaaaatca
    30121 agcagttgtg acatagcttt cattcgactc tcgttattaa tatcaaagtc agtcattctg
    30181 atattccgat aggacttggg tagattaact aaattaatac gatcactgat agcttgtttt
    30241 ttttgtgctt caattaactc tcgtgtttct aaataagaaa catctgcata accttcattc
    30301 ataaccaaaa taggctcata gcctttagca atatattgac tatccttatt tttaaatttc
    30361 tgacgctcaa tcaaaaattg attaaattta gacatgctga tatttatttc acgctgagaa
    30421 agttggtggg ttttaatgaa ctccgctacc tgagcatctg ctaaaatcat ttgaataagc
    30481 tcatcatttg tattacgagg cactctccct tgattttcta atgcttgtcc cactgatttc
    30541 atgattcacc atcctttcct aatcgttcta aacgcttgag ggcatctgtt ttaaactgtt
    30601 ctaattcaat ttctttttct gggctaacct catctttata atcaggatta ctccattcag
    30661 gaacattcga ttgtttcttc ttcgtttctg ttttagtcct tactttttga tctgaaaaat
    30721 cacgaatttt aagcacagca tcttcggccg ttataacatt ctgaaaggca aaatcattag
    30781 caactttcat gatataggcc ttatttaagt tagcagattt tgtcttattc aaggtatata
    30841 aaaccataac attaatgact tcgtctagga aattcatttt tgccaaatcc tcaagtaaag
    30901 tcttctcacc acttgttgtc actgccttac ggcttctctt gattttttca aggaacacta
    30961 gagcactatc atttttagat tcccttagta tgaccttttc attatttgta aaactctgat
    31021 tatctttaat atgctgcccc tcattttgtt gaagtttcat tcgctgaggc gcaatcattc
    31081 cattgatagc tgtttgtttt gctaattgat aggtatcata ccaatttaaa tgataaagtt
    31141 ctgatacact ataaatacct aagacatcat ctttttcatt gttaaaacgt aagccatctc
    31201 gcatcatcaa acgtttgaaa ctttccaagt caaaaacatt tttactccgg tttacctctt
    31261 gttttggttg tcctaaatcc gagaaaacat ctgtaaattt tttagagata tctctagcat
    31321 ttttaggtat tttcatatct aattctgcca ctgctacctc tcctatacgt ttttctaaca
    31381 aacgacgata gatgggatta gataaaaata actcatttga catagcttga ttcaacttaa
    31441 caaggtaagt acctgtggct tggtaaacag tcaacaaatc caaagctgtt aacattacca
    31501 atgcatcttg aaatcttggc atcccgtatt gcagatgatt gagaacctct gaaaatttat
    31561 gacgtcgtat tccgtcatca aaaaaatgaa caaaataatc atagagtgcc atggcatcac
    31621 ttcccataat tgggaaatac aattgtatta aactattact atcataaaca atcttatttt
    31681 gcttaatata tgtaaactca tctattggac tcatcacacg ctaccactct ttttactccg
    31741 tacgcgcttt gtaatttgtt gaagtagttc ttcaatttca tctacatctt taaaagattt
    31801 ataaacacta gcaaagcgca cataagtaat ctcatctaat tcggcgagtt catccatcac
    31861 taaatttccg atagtgatac tagaaacctc atcttcatac tcactcctaa ttttacgttc
    31921 gatacgtaag atacaatttt caatatcttc gctcgaaaca gggcgttttt gagcactctg
    31981 aataatacca ttcaaaattt tatctcgaga aaattgctcg cgagtaccat ctttctttat
    32041 gactaaaaga ggcaattctt ccaaacgttc aaatgttgta aagcggttgc cacatttttc
    32101 gcactctctc cggcgtctga tagtcgtacc ttcttcagct tgtcggctat caaccacact
    32161 agatttatta tagccacatt ttggacaacg catgtcaaca cccctattct atcatatcat
    32221 ttaatcatct tattttaaca caaattcagt aataacaaaa gaagcgcctc tcttatcacc
    32281 tcatcatatc aaaacacaaa ataatcctta tgccttttat cattaagcat aaggattttt
    32341 cttatatttc tttagtttct tcaaattttt ttataggaat gcgaacaatg aattcagtcc
    32401 cttgatttag ttcactttta atatccacct ttaaatggta agcatccata atttgtttaa
    32461 acactgaaag cccgatacct aaaccagctt gtgtactttc tctgttacgt gatttatcag
    32521 ttcgataaaa acgatcaaag ataaactcaa tatcttcatc tgagatacct tctcctttat
    32581 cttttacaac tactgtcgca aaatcattgt ctactgataa aacaacactg acttctttat
    32641 aactaggaga gtacttgata gcgttatcaa ttaagatcat gagagcttgc tcaaaatgta
    32701 tcttatatat ctttccccaa acgatatctg aaatattatt ttcaaaaata aattgaaaat
    32761 cttctctcaa aatacggaaa ttttcaataa caactgatat agagcttgat aaatctgtca
    32821 cttcatcttg atgaagttcc aagcttcctt ggacgcgaat catatttaac atgtcattaa
    32881 tcattaagga catacgatct gcctcatggt atgccgctgc taaactttct tcaagaattt
    32941 ctggatcatc tttcccccaa cgttgtaata aaccaatatg accctttaca acagcgacag
    33001 gcgttcttaa ttcatgacta acatcactaa tgaattgtga ttgtcgacga gtatagtctt
    33061 ccaactgatc caacatccca tcaaaaatcc tagacaattc tccaatttcg tcttctgaac
    33121 ggaccttact tctaatttct aaattactag gattttcaga tatctgatgc attaagtcgt
    33181 gtaaattttt aaccggacga acaatacgct tagtagcact attaataacg accaaggcta
    33241 aaacagtccc taaaacttcc atcataatca agaaaacaat gatgttttgc ctcatactat
    33301 aataacgacc taaattatag aaaatttgaa cgtatccaag caactgtcct gttttttgag
    33361 agtagattgc ttttgtacca acaaaacctt ccatatctgc aactttagag atgataactt
    33421 ttcccatgga tttaccaata gttggcatac ccgttctcag tgtagaaaaa actggtttcc
    33481 tgttgacatc gtatagggtc atttcttgat taacataaag aatactatcg attgactcgc
    33541 ccgttctaac aattccattt tgacgcttat gaggatagat gtcatccgca aaaacctggt
    33601 tgtacaggat ctctaaaata tcatcggatg tcaaactaga gttcgcttgc gacaaacgaa
    33661 ctctagtttt ttctaatgac ctaccgacat taattttttc cctgtgaagg acgtatcttg
    33721 ttgccgaagt ataaactaaa acagaaaata ctgtaaatag gctaaaaaat aagataaaaa
    33781 ctaattgcga taattttttt gacagaggtt gttttacccc tataaactga tcttttttat
    33841 ttttcacgaa tcacatagcc cattccgcga acagtttgaa tatatgattc acggcctggg
    33901 atatcaattt tacctcttag gtaacggata taaacatcaa caacgtttgt ctctgctgcc
    33961 acatcgtatt tccaaacatg ttctagcaac tcttctcgtg tcataacacg attcatattt
    34021 gtcatcaaga cattcaacaa atcaaattca cgttttgtta gtgaaatttc ctcatcacca
    34081 cgcatcgctg aacgattatg agtatttaat gacaaatcac ggaaactacc actatcacct
    34141 ttttcttttg tcttcgtttc aatttcttgg cgtcggaaaa tcgctctaac acgtgctaat
    34201 aattcttcga ttgcaaacgg cttaacaata taatcatctg ctccacgatc aagaccagct
    34261 acaatatcca taacagaatc acgtgctgtc atcatcatga tataggttgt tttttcagcc
    34321 tgcaggcgac gtgtgatttc gaaaccatcc atctctggaa gcattaaatc cagtagaatc
    34381 aaatcaaaat ctttttctaa tgctgtgtcc aatccttcac gaccgtttgt ttcaacaaca
    34441 acatcatatc cttcatgtag tagttctaac gagacgaagc gagctaaatt tttctcatct
    34501 tcgattatta agatcttttt acccatctac accaaccttc ttattcttta cttaacacta
    34561 tcaattattt tttagaatat ctagataaaa aacaagatag cccacatttt actaacgttt
    34621 ctttaaataa ataaatcgcc atcccaaaca actgttcgat agcgagactt aatttttatt
    34681 attgagtagt gtcctctgtc acttttcaat tctactaaaa aaaaattaaa atatcaactg
    34741 atgttattaa taatttttat ctaatcacta tcaaacatac cttctaaggc tgcgaaagga
    34801 ttagattttt ctttttcttt agcttgtttt tcttcatact gcttttcagt cattaacgac
    34861 caatttttac ctgataaatc cgcttctacc ccaacctcat cagctgccag aacacgaaga
    34921 ggtatattta acaagatatt atcgataacg ctttcttcta aattaatatc atcttcctct
    34981 aatattaaga ccaactcctg atcaacgagc tcttttttag tgctaacatt ttcagcttca
    35041 ataaaaactt cattaatagt ttgcttttca gataaaacaa ctggtttcat agaacgacta
    35101 gacggtaatg taatatcgta agccatatta tagtcaagta gatagagacc atcttcatac
    35161 accacatgac ctgaaacttg aatatcagaa atatccatta tctctgaatg acgctccata
    35221 agcgactctt tgatatctat ttttttatca aaataaagcc cctctggtga ttttttgatt
    35281 tctgtaagta acacgtgcca tctcccttta tagtatatgt catattatat caagttttac
    35341 aggctttgtc ttaaaaaaac aaaacgcaag gcatagctac cctacgtttt gttaacgact
    35401 acatatgacg caaacgctca atacgatctg caattggtgg atgcgtataa aataaagact
    35461 ttaacccttc ttttttcgtt gggtcattga tatagagagc cgcactagca tcatctactg
    35521 gatgccccat aggttcagaa cgatcaagtt tttctaaagc agagatcatt ccttgtggat
    35581 ttcttgttaa ctcaacagaa ctagcgtctg ctaagtattc gcgctgtctt gaaatagcta
    35641 attgaactaa actagcggct aatggtgcaa ggattagtga taaaatagag aaaatcaaaa
    35701 ccaagatgtt tccaccatct tctctgtcat catcacgcct acgacctccg ccgtaaaata
    35761 acatacggct accaatgctt gatattagag ttacagcact cgctaaagct actgcaatgg
    35821 ttgaaatacg gatatcataa ttacgaatat gactaacctc atgtccaata acaccctcta
    35881 gctcttcgcg gttcataaca gctaataggc ctgtcgttgc tgcaacagca gcattttctg
    35941 gcttagagcc tgtggcaaag gcatttaggg agtcatcttc aacaatgaaa acgcgtggca
    36001 taggtatctg agctatcatt gccatatctt caacaatatg aaaataattt ggcgcctcat
    36061 cttctgtaac ttcacgcgca ttgttcattg acataacaac attagttgat tgaaaaatca
    36121 tacttactgc atagataacc ccaataatta gagctagaac aagcccaaat tggtagctgc
    36181 ctaataccaa ataacctaca gctgctccaa tagctgctaa aagacagaag aagacaatta
    36241 acaagacaac tgtttttctc ttattacttg caatttgttg atacagcaaa agcctctctc
    36301 ccttctcaat taaaagtcaa aggaaacttt tggcacttct ttttcttctt caggagtttc
    36361 taagaattgg cttggtttga acccaaatag ttttccaaca atattacttg ggaaagtttc
    36421 taatttcaca ttataattgg atgttgttgt gttatataac tgacgtgaat aagaaatttt
    36481 attttccgta ttagttagtt cttcttgaag tttaacaaag ctattgttgg cttttaaatc
    36541 tgggtaattt tcagcaaccg ctaaaatact tgaaagctgt cttgttagct cattacttgc
    36601 tgtcattgct tcagcaggtg tgttagcttt agcaacttga gcacgcagtt ctgcaatctt
    36661 ctcaagtgtt ttcccttcgt acgcagcata gcctttaaca gtttcgatta ggtttggaat
    36721 taaatcgtta cgacgtttca attgaacgtc tatttgactc catgattctt tagtatgcat
    36781 gcgacttcta actaaactgt tataagcaac aatcaaccaa ataacaaaca aagcaataat
    36841 tgctattaaa atcatagttc ccatagtagt agctccttta cattttttcc attatagcaa
    36901 ttttctttag attaggaaag tcctgttatt cccattatta gtatttgtga taaaatagag
    36961 tcattatacg atatagaaat tgaggtttac tatgacaccc caagcatttt atcaagttct
    37021 gatagagcat ggtattacac ttactgacaa acagaaaaaa caatttgaaa cttattttag
    37081 acttcttgtt gaatggaatg agaaaattaa cctaacagct attacagata aggaagaggt
    37141 ttacttaaag cacttctatg attctattgc gccgatttta caaggttata ttgacaatag
    37201 tcctcttagc attttagata ttggtgctgg tgcaggtttt cctagtattc ctatgaaaat
    37261 c
//
LOCUS       DATROD010000010        35709 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.010, whole genome shotgun sequence.
ACCESSION   DATROD010000010 DATROD010000000
VERSION     DATROD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35709
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.010"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..1225
                     /locus_tag="VBC74_000509"
     CDS             <1..1225
                     /locus_tag="VBC74_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEO6941483.1"
                     /translation="PYLILSILGLIVIYSTTSATLIQLGANPFRSVINQGVFWAVSLV
                     AIIFIYKLKLNFLKNSKVLTMAVLVEVFLLLIARFFTQEVNGAHGWIVIGPISFQPAE
                     YLKVIIVWYLAFTFARRQKKIEIYDYQALTKGRWLPRSLSDLKDWRFYSLFMIGLVIA
                     QPDLGNGSIIVLTVIIMYCISGIGYRWFSALLGLIVVGSTLFIGTIAVVGVETMAKVP
                     VFGYVAKRFNAFFDPFKDLTDSGHQLANSYYAMSNGGWFGRGLGNSIEKLGYLPEATT
                     DFVFSIVIEELGVIGAGFILALVFFLILRIMHVGIKAKDPFNSMIALGIGAMLLMQVF
                     VNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAIGFVLNIDANEKKELIMKEAEEQYK
                     PQEKNEKIINLDAFK"
     gene            1577..2773
                     /gene="tuf"
                     /locus_tag="VBC74_000510"
     CDS             1577..2773
                     /gene="tuf"
                     /locus_tag="VBC74_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEO6941484.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPTSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDEKYEDIIMELMSTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEVEIVGIKEDIQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVLAKPGSINPHTKFKGEVYILSKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIEVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
     gene            2954..3712
                     /locus_tag="VBC74_000511"
     CDS             2954..3712
                     /locus_tag="VBC74_000511"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEO6941485.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFIEAVASKLPSSELVEAGIAAP
                     ALTLSTVLEAAKGSELKIAAQNSYFENSGAFTGENSPKVLAEMGTDYVVIGHSERRDY
                     FHETDQDINKKAKAIFANGLTPIICCGESLETYEAGKAVEFVGAQVSAALAGLSEEQV
                     SSLVIAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQAVADKVRVQYGGSVKPE
                     NVAEYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            3889..4581
                     /locus_tag="VBC74_000512"
     CDS             3889..4581
                     /locus_tag="VBC74_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEO6941486.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIQ
                     AAGIEFDLAFTSVLKRAIKTTNLALEAADQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPDMAKDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKQLSDDEIMDVEIPNFPPLVFEFDE
                     KLNLVSEYYLGK"
     gene            4710..6755
                     /locus_tag="VBC74_000513"
     CDS             4710..6755
                     /locus_tag="VBC74_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEO6941487.1"
                     /translation="MLNRKKRYRLTVKKQNASIPRRLNLLFFIIVLLFTVLILRLEQM
                     QIGQQSFYMKKLTALTSYTVKESKARGQIFDAKGVVLVENDERPTVAFSRGNNISSQS
                     IKELANKLSHYITLTEVASSDRAKRDYYLADKANYKKVVESLPDSKRYDKFGNHLAES
                     TVYANAVAAVPVSAINYSEDELKVVALFNQMNATPTFGSVKLSTGELSDDQIKKLDAD
                     KKELLGISVTSNWHRRKKGTSLSDILGTISTEKAGLPREEVKKYLKKGYSLNDRVGTS
                     YLEKQYEDDLQGIRQIRKVVVNKKGKVVSDNITQEGKSGRNLKLTIDLNYQNKVESIL
                     KQYYGSELSSGRASFSEGMYAVAIEPSTGKVLAMAGLKNDHGNLVDDSLGTIAKNFTP
                     GSVVKGATLSSGWENKVLRGNEVLYDQEIANIRSWFTRGLTPISAAQALEYSSNTYMV
                     QVALRLMGQDYNTGDALTDRGYQEAMAKLRKTYGEYGLGVSTGLDLPESEGYVPGKYS
                     LGTTLMESFGQYDAYTPMQLGQYISTIANNGNRLAPHVVSDIYEGNDSNKFAQLVRSI
                     TPKTLNKIAISDQELAIIQEGFYNVVNSGSGYATGTSMRGNVTTISGKTGTAETFAKN
                     INGQTVSTYNLNAIAYDTNRKIAVAVMYPHVTTDTTKSHQLVARDMIDQYISQFTGQ"
     gene            6770..7366
                     /gene="recR"
                     /locus_tag="VBC74_000514"
     CDS             6770..7366
                     /gene="recR"
                     /locus_tag="VBC74_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEO6941488.1"
                     /translation="MLYPTPIAKLIDSFSKLPGIGTKTATRLAFYTIGMSDEDVNEFA
                     KNLLAAKRELTYCSVCGNLTDDDPCLICTDKTRDQSVILVVEDSKDVSAMEKIQEYNG
                     LYHVLHGLISPMNGISPDDINLKSLITRLMDGQVTEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            7507..8553
                     /locus_tag="VBC74_000515"
     CDS             7507..8553
                     /locus_tag="VBC74_000515"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEO6941489.1"
                     /translation="MSKETLILLYGGRSAEREVSVLSAESVMRAINYDKFFVKTYFIT
                     QVGQFIKTQEFDEMPSSDEKLMTNQTVDLDKMVRPSDIYDDNAIVFPVLHGPMGEDGS
                     IQGFLEVLRMPYVGTNILSSSVAMDKITTKQVLATVGVPQVAYQTYFEGDDLEHAIKL
                     SLETLSFPIFVKPANMGSSVGISKATDESSLRSAIDLALKYDSRILIEQGVTAREIEV
                     GILGNNDVKTTFPGEVVKDVDFYDYDAKYIDNKITMDIPAKVDEATMEAMRQYASKAF
                     KAIGACGLSRCDFFLTKDGQIFLNELNTMPGFTQWSMYPLLWENMGLTYSDLIEKLVM
                     LAKEMFEKRESHLI"
     gene            8700..10067
                     /locus_tag="VBC74_000516"
     CDS             8700..10067
                     /locus_tag="VBC74_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEO6941490.1"
                     /translation="MKLSLHEVAKVVGAKNQVSEFEDVPLGNIEFDSRNISEGDLFLP
                     LKGARDGHEFIEMAFDNGAIATISEKEIEGHPYLLVSDALKAFQVLAQYYIEKMNVDV
                     IAVTGSNGKTTTKDMIAAILSTTYKTYKTQGNYNNEIGLPYTVLHMPEDTEKIVLEMG
                     QDHLGDIHVLSEIAKPRIAVVTLIGEAHLEFFGSREKIAEGKMQITDGMSSDGILIAP
                     GDPIIDPYLPANQMTIRFGHDQELQVTELKEEKHSLTFKTNALEHQLRIPVPGKYNAT
                     NAMVAAYVGKLLAVAEEDIVDALENLQLTRNRTEWKKSANGADILSDVYNANPTAMRL
                     ILETFSAIPNNDGGKKIALLADMKELGEQSVDLHNQMIMSIRPDSIDTLICYGQDIEG
                     LAQLASQMFPIGKVYFFKKNQEVDQFDQLLAKVKDTLKEKDQILLKGSNSMNLSKIVD
                     ILEVG"
     gene            10254..11474
                     /locus_tag="VBC74_000517"
     CDS             10254..11474
                     /locus_tag="VBC74_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEO6941491.1"
                     /translation="MKNLNRYVVAVSGVVLHLMLGSTYAWSVFRNPIISETGWDISSV
                     SFAFSLAIFCLGMSAAFMGHLVERFGPRIMGMISAILYGAGNVLTGLAIETQQLWLLY
                     VAYGILGGIGLGSGYITPVSTIIKWFPDRRGLATGFAIMGFGFASLVTSPLAQSLLIR
                     IGVGKTFYILGLVYFFVMMIASQFIKQPPQEKITILTHDGKKNAMNSQIITGLKANVA
                     IKSKTFYIIWLTLFINISCGLGLISAASPMAQDLAGYSAESAALLVGVLGIFNGFGRL
                     LWASLSDYIGRPLTFIILFIVNFIMTSSLFLSFNAIVFAIAMSILMTCYGAGFSLLPA
                     YLSDIFGTKELATLHGYSLTAWAIAGLFGPLLLSKTYSWGNSYQLTLMVFGFLFLFGL
                     LLSLYLRKLTTKVV"
     gene            11603..12289
                     /locus_tag="VBC74_000518"
     CDS             11603..12289
                     /locus_tag="VBC74_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HEO6941492.1"
                     /translation="MIEFFSNIRTEIPQMPLLIHSLILSVLPFLMWLTLVNRDKPLYK
                     TIWSILLGLQLITIYTWFFWAKLPLSESLPLYHCRIGMFVVLLARPRILKDYFALLGV
                     VGGVLAMIHPDFYPYQFLHVTNIFFFIGHFALFVLSLLHLMTQSNLDKLNPKLIIQLT
                     LLINMSLIFINLLTGGNYGFMMKTPILGITNPFLNLLIVTTLLSFLVLFVKQIFQKVR
                     MRASFKTEES"
     gene            12469..14006
                     /locus_tag="VBC74_000519"
                     /pseudo
     CDS             12469..14006
                     /locus_tag="VBC74_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            14183..15727
                     /locus_tag="VBC74_000520"
     CDS             14183..15727
                     /locus_tag="VBC74_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEO6941493.1"
                     /translation="MTLQDEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLDLLEELEEVLG
                     IASYPMNWPIGMGKSFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNDFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VIDPLAKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPELFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWINSDDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     KLEEKM"
     gene            15813..16193
                     /locus_tag="VBC74_000521"
     CDS             15813..16193
                     /locus_tag="VBC74_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEO6941494.1"
                     /translation="MGIFSGLMGNASQVDTDKVENQLSDILIPGEQVDLAYTLIRDLI
                     VFTNYRLILVDKQGVTGKKVSYNSIPYASISRFTVETSGHFDLDAELKIWISSAIEPA
                     EVLQFKNDRNIVSIQKALAIAVLK"
     gene            16387..17121
                     /locus_tag="VBC74_000522"
     CDS             16387..17121
                     /locus_tag="VBC74_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6941495.1"
                     /translation="MIIELKSVGKIFPKSQFRLDDISFNIAEGEIVGLIGSNGTGKST
                     ILKMINGLIPYDKGNIYYQGKEVKSFSDNKLRQMRKDIAYIFQNHNLLAGESVYYHLA
                     LVYKLNHQKVNHDAINDILDFLGLMDLKQVKCHSLSGGQQQKVAIAMAVLQKPKLILC
                     DEISSALDTNSEKEIFNLLSDLREKYGISILMIAHHLSLLKQYCDRVMILDHQTIVDT
                     VVPVKATLNQLKSNYVDQVKEFLLHD"
     gene            17114..17776
                     /locus_tag="VBC74_000523"
     CDS             17114..17776
                     /locus_tag="VBC74_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6941496.1"
                     /translation="MIDIIEKHGEGILKSFWETNLMLGLTLILCFLIAFPTGILLFSL
                     RKSYLIKHSLAYQLLNLFLGTLRSVPFLIFIFILIPLNRLIFGTSFGTIAAILPLTLV
                     SVSLYARYVEQALLNIPQVVVDRALSLGANKRQIIYYFLIPSIKIDLVLSFTATAISI
                     LGYSTIMGVIGAGGLGEYAYRFGYQEYDYPVMYLIVVLFIIYVFILQSLGYFIANRYS
                     RK"
     gene            17792..18622
                     /locus_tag="VBC74_000524"
     CDS             17792..18622
                     /locus_tag="VBC74_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941497.1"
                     /translation="MKKLLSLTCLIMMSLCLVACTKQAMSSKQIKDKNSKEKVITVAT
                     YSKPTSTFLDLIKDNVKEKGYTLKVVMVSDYIQANIALENKEHDANLLQHEFFMSIFN
                     KENDGHLVSITPIYHSLAGFYGQHLKNIAELKDGAKVAIPSDPANMTRALLLLQEKKL
                     ITLKNTSKKTKAIEDIITNPKKLRIEPVALLNLNQAYFEYDLVFNFPGYVTKINLVPK
                     RDRLLYEKKPDIRFAGALVAREDNKNSDKIKVLKEVLTSKEIRHYITKEIPSEADVAF
                     "
     gene            18857..20443
                     /locus_tag="VBC74_000525"
     CDS             18857..20443
                     /locus_tag="VBC74_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6941498.1"
                     /translation="MKFTELNLSQDILSAVEKAGFVEPSPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIHTEDNTIQALIIAPTRELAVQSQEELFRFGRDKGVKVRS
                     VYGGSSIEKQIKALRSGAHVVVGTPGRLLDLIKRKALKLNHIETLILDEADEMLNMGF
                     LEDIEAIISRVPEARQTLLFSATMPDPIKRIGVKFMKDPEHVKIKATELTNVNVDQYY
                     VRVKENEKFDTMTRLMDVDQPELSIVFGRTKRRVDELTRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDHIDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVSPNEMGYLTIIENLTKKRMTGMKPATASEAFQAKKKVALKRIARDFEDQ
                     ELVSKFDKFKADALELATQYTPEELALYVLSLTVQDPESLPEVEITREKPLPFKPSGG
                     GFKGKGGRGNGRGGDRRRNDRGDRRGNRDRDDRGGRRDFKRRDDKFKKDNRRQENKKP
                     HKNTSSEKQTGFVIRNKGDK"
     gene            complement(20626..20892)
                     /locus_tag="VBC74_000526"
     CDS             complement(20626..20892)
                     /locus_tag="VBC74_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEO6941499.1"
                     /translation="MIKVPAYMYVLECSDGTLYTGYTTDVKRRLNTHNTGKGAKYTRA
                     RLPVKLLYSEAFNSKQEAMRAEALFKQKTRKAKLTYIKQHKNEQ"
     gene            complement(20885..21649)
                     /locus_tag="VBC74_000527"
     CDS             complement(20885..21649)
                     /locus_tag="VBC74_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEO6941500.1"
                     /translation="MIDTILKENERIDQLFSTDVKIIQNKEVFSYSIDSVLLSRFPKL
                     PSRGLIVDLCSGNGAVGLFASTKTSATIIEIELQERLADMAKRSIKLNKLEKQVTMIN
                     DDLKNLLDHVQRSNVDLMLCNPPYFKASETSKKNLSPHYLLARHEITTNLREICQIAQ
                     HALKTKGRIAMVHRPDRFLEIIDTMHQFNLAPKRIQFVYPKLGKDANMLLIEAIKDGS
                     TEGMKILPPLVVHQDNGDYTETIFDIYFGENGKSHD"
     gene            21784..22524
                     /locus_tag="VBC74_000528"
     CDS             21784..22524
                     /locus_tag="VBC74_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEO6941501.1"
                     /translation="MFYTYLRTLVMFLIWVANGNAHYHNEDKMLKDDENYILVAPHRT
                     FWDPVYMAFAARPKQFVFMAKKELFTNRLFGWWIKMCGAFPIDREKPGQDAIRYPVKM
                     LKNSNRSLVMFPSGSRHSKDVKGGVAVIAKMAKVRIMPAAYRGPMVFKNLLKGHRVDM
                     NFGNPIDVSDIKRMDAEGIAEVSRRIQEEFDRLDRENETYDDGKKLNPLTYIYRLPLA
                     IIAIVLLVLTLIFSYLASFVWDPQKHLK"
     gene            22624..23277
                     /locus_tag="VBC74_000529"
     CDS             22624..23277
                     /locus_tag="VBC74_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEO6941502.1"
                     /translation="MFEIVLEKIKSHKWETTGIIVGLLLFGILGLNHFGTHHKEDDLN
                     INLEKKVSTITEKKVPMISHVKDKVSNQVTVDVKGAVNHPGVYSLPSQSRVTDAIKRA
                     GGLSNLADSKSVNLAQKLQDETVIYVAQKGEKITVVEEEKANNIATQGNSKGKINLNK
                     ADLSSLQTISGVGAKRAQDILDYRDSQGGFKTIDDLKNVSGIGEKTLEKLRQDVTLD"
     gene            23261..25498
                     /locus_tag="VBC74_000530"
     CDS             23261..25498
                     /locus_tag="VBC74_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEO6941503.1"
                     /translation="MLHLTKYFPLKPIYLALLVFQIYLLVFSWTMLGCAFLLFTFIFL
                     IYQYDRETIFKTITIVIFFLFYFLWQNHNMNVQYQRVPNHISQIKVRIDTISINGDVL
                     SFQADASGNTYQAFYTLKNKSEKDYFQNLDNNIMIIADIKLEEAEERRHFNGFDYRQY
                     LKRHGIYRIAKVTKIKQIRLFQHRSFFDLMFKWRRSAIVISQTFPNPMRHYMSGLLFG
                     YLDKTFDDMSDLYSSLGIIHLFALSGMQVGFFLGIFRYICLRIGLRLDHVWLLQIPFS
                     LIYAGLTGFSISVVRALIQSLLSHSGVKKDENFALCLLICLISLPHSLLTTGGVLSFA
                     YAFILTMTSFDHFSSIKKVAIESLTVSVGILPILTYYFSGFQPISIILTALLSFAFDI
                     IFLPLLTVIFVLSPFVKLSCINSLFEILEVLLKWTGQLFPRPLIFGKPSLFLLIVMII
                     ILGLLYDYYHSKCFRYCSLLIIFTLFFITKNPITNEVAILDVGQGDSILVRDWLGKTI
                     LIDTGGRVRFEQPEEWKQKVNQSNAERTLIPYLKSRGISKIDNLVITHTDTDHMGDME
                     VISKHFKVARLITSSGSLTNSQYVKRLSKIGVAVKSIEAGDKLSVMGSYLQVLYPWHK
                     GDGKNNDSIVLYGHLLGKGFLFTGDLEEEGEKQLLEAYPHLSVDILKAGHHGSKGSSS
                     LSFLKKLSPSVVLVSAGKNNRYQHPHQETLQRFQKIKSKIFRTDQSGTIRLTGWWKWH
                     IQTVR"
     gene            25624..26433
                     /locus_tag="VBC74_000531"
     CDS             25624..26433
                     /locus_tag="VBC74_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6941504.1"
                     /translation="MTIKRIFCDMDGTLLNSEGQVSKSNATLIREAAIPVTLVSARAP
                     MEMKDAVDALQLGGVQVAFNGGLIYRIGDNNQVLPIHTQIIKKSTVKQLLRGIRFHFP
                     QVSLSYYDLNNWYCDKIDEGIRYEHSLTQQCPTFIHNEDQFLEGHTNTFKIMMITFDE
                     ANMLELEKYLQSLELPEITIQRSGKAYLEITHLLAKKSKGIAYILQKEQLAREETAAF
                     GDGHNDLPMLEMVGYPIVIDNAFDDIKAIAYQLTKSNDEDGVGYGIQKFLK"
     gene            26444..27388
                     /locus_tag="VBC74_000532"
     CDS             26444..27388
                     /locus_tag="VBC74_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6941505.1"
                     /translation="MTSISDIAKKAGVAKSTVSRVINHHPHVSDETRQKVMALITELD
                     YIPNQLARDLSRGKTQKIGVVIPHTRHPYFTQLINGLLDAAKTTDYQLVVMPSDYNQE
                     LELSYLKQLKMEAIDALIFTSRAISLDIIETYAKYGRIVVCEKLQEYNHLSSAYLDRY
                     SSFLEAFSDMKLRGLEHLVLLFSRNNESSATYQSALLAYQEVYGQLSSPYMVVGNVHD
                     FNDGLNLSYQLVKEVSIDGILATSDEVAAGLIKGYEESRKKCPYIIGQECLLVGQLLK
                     LPTIDHKSYYLGKLAFKQALAEKISQEVLFSEFLSQDN"
     gene            complement(27447..28439)
                     /locus_tag="VBC74_000533"
     CDS             complement(27447..28439)
                     /locus_tag="VBC74_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6941506.1"
                     /translation="MKNYRKLIVLLLLIFFAIFMGAYAYTHIVEKRSLTSNTIEKTLP
                     VVNQIKPQTIKEYQNYLTKVAKRNVLPVDIPQALNNEKVEITATDGMQTFTWNDKNNP
                     KQKVIFYVHGGSYIHQASELQYIFVNKLAKKLDAKVVFPIYPKAPTYNYSDAIPKIKK
                     LYQNTLASVTSHKQIILVGESAGGGLALGIADNLVTEHIKQPKEIILISPWLDIATNN
                     PKIEKVQKKDPLLKAWQLQQVAPYWANGKKNFKNPQVSPLYSSQFNKMAPISFFIGTH
                     DIFYPDNQLLHQKLAKENIKHHYIVGQKMNHVYPVLPIPEAETAFKQMLKIINH"
     gene            28615..29343
                     /locus_tag="VBC74_000534"
     CDS             28615..29343
                     /locus_tag="VBC74_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6941507.1"
                     /translation="MFHREDFSKALLKKALQPGMRVMDIGCGSGELTRLAADIVGKEG
                     DVVGIDINEQLLWQARHDNGYANVSYQNADLAALSSFETKSFDAIIGRRVLMYQKNPK
                     KVIESLTPLLKQNGFYVFQESDAMCLELAKPSFVNMNKIQSLIWQTVEKEGGHIHIGS
                     QLEQLMLEAGLIVDHFVSENVIQTSQTGTDLSWLLNIMKERIQKYSDLQGLEDVNLQK
                     ELEENNDHFIRDCQFGIISHRVDE"
     gene            29397..30434
                     /locus_tag="VBC74_000535"
     CDS             29397..30434
                     /locus_tag="VBC74_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEO6941508.1"
                     /translation="MIAIEEIGRITPDNLGLVTVLAGEDLGQYAQMKEKLFQVIGFNK
                     DDLAYSYFDLSEEEYQNAELDLESLPFLSDYKVVIFDQFQDITTDKKTYLDEQAMKRL
                     EAYLQNPVDTTRLVICAPGKLDGKRRLVKLLKRDARVLEANTLKESDLKTYFQKYAHQ
                     EGLVFEAGVFDELLIKSNYDFSDTLTNIAFLKSYKTDGHISSNDVREAIPKSLQDNIF
                     DLTQDVLLGRIDLARDLVRDLRLQGEDEIKLIAIMLGQFRMFLQVKILASKGKSESQI
                     VSELSHYIGRKINPYQVKFAVRDSRNLPLAFLKEAIRILIETDYAIKRGTYDKDYLFD
                     LALLKIAHKKP"
     gene            30514..31122
                     /locus_tag="VBC74_000536"
     CDS             30514..31122
                     /locus_tag="VBC74_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEO6941509.1"
                     /translation="MAIILPDLPYAYDALEPHIDAETMTLHHDKHHATYVANANAALE
                     KHPEIGEDLEALLADVSQIPEDIRQAVINNGGGHLNHALFWELMSPEETQISQELSED
                     IDATFGSFEDFKAAFTAAATGRFGSGWAWLVVNAEGKLEVLSTANQDTPIMEGKKPIL
                     GLDVWEHAYYLNYRNVRPNYIKAFFEIINWNKVNELYQAAKA"
     gene            31460..32311
                     /locus_tag="VBC74_000537"
     CDS             31460..32311
                     /locus_tag="VBC74_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEO6941510.1"
                     /translation="MIIKRVLNHNAVISVTHQGLDVLLMGKGIAFKKRIGDRINSDAI
                     EKSFVLKNSDNMNRFTELFITVPEEVVACSERIINLGKIKLGKNLDEILYINLTDHIH
                     SAIERHEQRMVIQNPLRLEIQRYYPDEYSIGMKALELIKDELGICLTIDESAFIAMHF
                     VNAGLDNPFNEAHKITEIVSYIEQKVKIDFRTELDESSIDYYRFMTHTKLFAQRVLSG
                     MKYEDDDADLLLVVKKKYPREYKCVKEIGNNMAIQYQYQLNSSELLYLTVHVKRLVKN
                     LKEISDD"
     gene            32304..34172
                     /locus_tag="VBC74_000538"
     CDS             32304..34172
                     /locus_tag="VBC74_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEO6941511.1"
                     /translation="MTKYQETAKAILAAVGGEKNIQHVTHCVTRLRLVLDNDEIVNDQ
                     VIKTIPNVIGVMRKNDQYQIILGNDVNNYYNAFLALGHFENTTREFSSQKKSSIFEKL
                     IETIAGVIKPLIPALLGGGMLKVIGILLPMLGITSSSSQTVAFINFFGDAAYYFMPIM
                     IAYSAASRFKVTPVLAATVGGILLHPAFVTMVAEGKPLSFFGAPVTLASYGSSVIPIL
                     IMVFLMQYIERWINKIVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGKGLSSAML
                     SIYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVTTPDVLILPAMLASNLAQG
                     AASLAVAVKAKQKQTRQVAFAAGLSALLAGITEPALYGVTLKFKKPLYAAMISGGLVG
                     AYIGLVNIASYTFVVPSIIGLPQYINPQGGNNFSNAVIVAIATIILTFIITWFLGIDE
                     GENEKSSINAQEYTHIRSGLSKKETLYSPMVGNVLPLSKVPDETFSSKLLGEGLAITP
                     SVGEVYAPFDGEIISLFPTKHAIALKDDKGVEVLIHIGIDTVELNGEGFEQLVKVGDF
                     VKRGQLLLRMDIDFISSKGYSLISPVVVTNSIDQLEIIVKDEETMVTNEDDLLVIL"
     gene            34189..35616
                     /locus_tag="VBC74_000539"
     CDS             34189..35616
                     /locus_tag="VBC74_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEO6941512.1"
                     /translation="MTIFPKHFLWGGAVAANQVEGAFRTDGKGLSVQDVLPNGGLGDF
                     TAKPTPDNLKLEAIDFYHNYKNDIKLFAEMGFKVFRTSIAWSRIFPNGDDSAPNEAGL
                     QFYDNLFDELLKYNIEPLVTLSHYETPLHLAKTYNGWADRRLIAFFEKFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGAIMADKSQLSPQELYQAIHHELVASARVTKLGRSI
                     NPNFKIGCMILAMPAYPMTSDPRDVLAARQFEQHNLLFSDIHVRGKYPTYIQSYFKNN
                     GIEIKFEEGDEEVLAQNTVDFLSFSYYMSVTQAYDFENYQSGQGNILGGLTNPYLTTS
                     EWVWQIDPIGLRLVLNQYYERYQIPLFIVENGLGAKDQLIETLDGDYTVEDDYRIDYM
                     NQHLVQVAKAIEDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGTGSLQRY
                     KKKSFGWYQKVIKTNGQSLFEHHNR"
BASE COUNT        11674 a         5435 c         6942 g        11658 t
ORIGIN      
        1 tccctatttg atattgtcta ttctaggctt aattgtaatt tattcgacaa ctagtgcaac
       61 tctaattcag cttggagcta acccttttcg ttcagtaatc aaccagggtg tcttttgggc
      121 agtcagtttg gttgcaatta tctttatcta taaactgaaa ctaaattttt tgaaaaattc
      181 taaagtgttg actatggcag tattggtaga agtatttttg ctactaatag cgcgcttttt
      241 cacacaagaa gttaatggag cacatggttg gattgttatt ggtcctatta gttttcaacc
      301 tgctgaatat ttaaaagtta tcattgtatg gtatttagcg tttacttttg ctcgaaggca
      361 aaaaaagata gaaatatatg attatcaagc tttgacaaaa gggagatggc ttccgagatc
      421 actaagcgac ctaaaagatt ggcgatttta ttctttattt atgattggtt tggtaattgc
      481 acaacctgat ttaggaaatg gttcgataat tgttctaacg gtcattatta tgtactgtat
      541 tagtggtatt ggttatcgat ggttttcagc tttacttggt ctaatcgtcg tggggtcaac
      601 tttatttatc ggaactattg cagttgttgg agttgagaca atggctaaag tccctgtctt
      661 tggttatgta gctaagcgat ttaatgcttt ttttgaccca tttaaagatt taacagattc
      721 aggacaccag ttggctaatt catattatgc aatgagcaac ggtggctggt ttggtcgagg
      781 acttgggaat tctattgaaa aattagggta cttaccagaa gcaacaacag actttgtctt
      841 ttcaattgtt attgaggaat taggagttat tggtgcaggg tttattttag ctttggtttt
      901 cttcttaatt ttacgtatta tgcacgtagg tataaaggct aaagatcctt tcaactcaat
      961 gatcgctctt ggtattggag caatgctatt gatgcaagtt tttgttaaca ttggtggtat
     1021 ttctgggtta atcccctcaa ctggagtgac atttcctttc ttatcccaag gagggaacag
     1081 cctcttggta ctttctgtag ctatcggatt cgtgcttaat attgatgcta acgagaaaaa
     1141 ggaactcata atgaaagaag cagaagagca atataagcca caagaaaaaa atgaaaaaat
     1201 aatcaacctt gatgccttta aataatcatc caaacaaaaa acgagatttt tatctcgatt
     1261 tttgtatttt ttgaaattta aactctaatt aatagagaga agatatttga agaaaagaag
     1321 tgtctattaa tattctaata ctataaatta ggatgtattt aacaatatga ttatatgata
     1381 actactaaaa aatacatata agagataaca aacgcctcaa tcaatcttac ccgaatttta
     1441 aggaaataaa gaaataagca aaaaagcata aaatacttgc ttttttagag gattttgtta
     1501 aaatagataa gtaaagttag actgtattgc ctaccgtcta tctataaaat atattttatt
     1561 ggaggctttt cctaaaatgg caaaagaaaa atacgatcgt agtaaaccac acgttaacat
     1621 tggtacaatc ggacacgttg accacggtaa aactacttta acagcagcta tcacaactgt
     1681 attggcacgt cgcttgccta cttcagttaa ccaaccaaaa gattacgctt ctatcgatgc
     1741 tgctccagaa gagcgcgaac gcggaatcac aatcaacact gcacacgttg agtacgaaac
     1801 tgaaaaacgt cactatgccc acatcgacgc tccaggacac gcggactacg ttaaaaacat
     1861 gattactggt gctgctcaaa tggacggagc tatccttgta gttgcttcaa ctgatggacc
     1921 aatgccacaa actcgtgagc acatccttct ttcacgtcaa gttggtgtta aacaccttat
     1981 cgtattcatg aacaaagttg accttgttga tgatgaagaa ttgcttgaat tggttgaaat
     2041 ggaaattcgt gaccttcttt cagaatacga cttcccaggt gatgaccttc cagttatcca
     2101 aggttcagct cttaaagcac ttgaaggcga tgaaaaatac gaagacatca tcatggaatt
     2161 gatgagcact gttgatgagt acattccaga accagaacgt gatactgaca aacctttact
     2221 tcttccagtc gaagatgtat tctcaatcac tggacgtggt acagttgctt caggacgtat
     2281 cgaccgtggt actgttcgtg tcaacgacga agttgaaatc gttggtatta aagaagatat
     2341 ccaaaaagca gttgttactg gtgttgaaat gttccgtaaa caacttgacg aaggtcttgc
     2401 aggggacaac gttggtgttc ttcttcgtgg tgttcaacgt gatgaaatcg aacgtggtca
     2461 agttcttgct aaaccaggtt caatcaaccc acacactaaa tttaaaggtg aagtttacat
     2521 cctttctaaa gaagaaggtg gacgtcatac tccattcttc aacaactacc gtccacaatt
     2581 ctacttccgt acaactgacg taacaggttc aatcgaactt ccagcaggaa cagaaatggt
     2641 tatgcctggt gataacgtta ctatcgaagt tgaattgatt cacccaatcg ccgtagaaca
     2701 aggtactact ttctcaatcc gtgaaggtgg acgtactgtt ggttcaggta tcgtttcaga
     2761 aatcgaagct taatttaact taattaagtt cccaaacggc agttagagtc agacagctca
     2821 aattgtgaag agtcctcagg gactcttttt tagtatctat aattaaagcg ttttactaga
     2881 ctcgtgaaac ttttaattaa ctataattta tggtatgata gattagtaaa taaaaaaaga
     2941 agaggtatgt gtaatgtcac gtaaaccatt tatcgctggt aactggaaaa tgaataaaaa
     3001 tccagaagaa gcaaaagcat tcatcgaagc agtagcttct aaattaccat catcagaact
     3061 tgttgaagca gggattgctg ctccagcact tactttatca acagttcttg aagcagctaa
     3121 aggttcagaa ttgaaaatcg ctgctcaaaa tagctatttt gagaattcag gtgcattcac
     3181 tggtgaaaac agccctaaag tattggctga aatgggtaca gactacgttg ttatcggtca
     3241 ctcagaacgt cgtgattatt tccatgaaac tgaccaagat atcaacaaaa aagcaaaagc
     3301 tatctttgct aatggtttga ctccaatcat ttgttgcggt gagtctcttg aaacttacga
     3361 agctggtaaa gcggttgaat tcgtaggtgc tcaggtttca gcagctcttg caggtttgtc
     3421 agaagagcaa gtatcatcac ttgttatcgc ttacgaacca atctgggcta ttggtactgg
     3481 taaatcagca actcaagacg acgcacaaaa catgtgtaaa gcagttcgtg acgttgttgc
     3541 agcagacttc ggtcaagcag ttgctgacaa agttcgcgtt caatacggtg gttcagttaa
     3601 acctgaaaac gttgcagaat atatggcttg cccagacgtt gacggtgccc ttgtaggtgg
     3661 agcttcactt gaagcagaaa gcttccttgc cttattagat tttgttaaat aaatcacatg
     3721 atatctaaag gcatagcact ctaggtgata tgccttttct tatagatagt taataataag
     3781 ctatttttga ttattgaact agtaatcaga ctagattttt atggctattt tttgtagaat
     3841 agttaaagta aatattggta tagccaaaat taaaggagaa attttctaat ggtaaaatta
     3901 gtattcgcac gccacggtga atctgagtgg aataaagcta accttttcac tggatgggct
     3961 gacgtagatc tttcagaaaa aggtacacaa caagctattg atgctgggaa attaattcaa
     4021 gcagcaggta ttgagttcga ccttgctttt acatcagttc ttaaacgtgc catcaaaaca
     4081 actaaccttg cccttgaagc agctgatcaa ctttgggtac cagttgaaaa atcatggcgc
     4141 ttgaacgaac gtcattacgg tggattgaca ggaaaaaata aagcagaagc agctgaacaa
     4201 tttggtgatg agcaagttca tatttggcgt cgttcatatg atgtattgcc tccagatatg
     4261 gctaaagatg atgaacattc agcacatact gatcgtcgct atgcttcact agatgattct
     4321 gttattccag atgcagaaaa cctaaaagtt actttagagc gtgctcttcc tttctgggaa
     4381 gataaaattg ctcctgctct taaagatggt aaaaatgtgt ttgttggtgc acacggtaac
     4441 tcaatccgtg ctcttgtaaa acatatcaaa caattgtcag atgatgaaat catggacgtt
     4501 gaaattccta acttcccacc acttgttttc gaatttgatg aaaaattaaa ccttgtttca
     4561 gaatattact taggtaaata atctaatctt tatataaaaa attcaggcca agttggcttg
     4621 aattttttat tatttataac attttgagtg tctgtaaaaa accattttta tgatacaata
     4681 gtagtcagag tttttatagc aaggagttaa tgttgaatcg taaaaaaagg tatcgtctca
     4741 ctgtaaaaaa gcaaaatgct agcataccta gacgattaaa tcttctattt tttattattg
     4801 ttttattatt tactgttcta attcttcgtt tagagcaaat gcagattggg caacaatcat
     4861 tttacatgaa aaaactgaca gctcttacga gttatacagt gaaagaatca aaagcgagag
     4921 gtcagatttt tgatgctaag ggagttgtct tagttgaaaa tgatgaacgt cctactgtgg
     4981 cgttttctag aggaaacaat atttcatctc agtctatcaa agagttagcg aataaattat
     5041 cacattacat taccttaacc gaagttgctt caagcgatag agcaaagaga gattattatt
     5101 tagcagataa agccaactac aaaaaagttg ttgaaagttt accagattct aaacgttacg
     5161 ataaatttgg taaccatcta gccgaatcaa ccgtatatgc aaatgcagtt gctgcagttc
     5221 cagtgagcgc tattaactat tctgaagatg agttaaaagt ggttgcctta tttaaccaaa
     5281 tgaatgcgac accaacgttt ggttcagtga aattaagtac aggtgaatta agtgatgatc
     5341 aaatcaagaa actggatgct gataagaagg aactgttagg aatttctgta acaagtaatt
     5401 ggcatcgtcg taaaaaagga acttctttat cggatatatt aggtactatt tctacagaaa
     5461 aggcaggttt accaagagaa gaagttaaaa aatatttgaa aaaaggctat tcactgaatg
     5521 accgagtggg aacttcttac cttgaaaaac aatatgaaga tgacttacaa ggaatcagac
     5581 aaattcgcaa agtagttgtt aataaaaaag gtaaagttgt ttcagataat attacgcaag
     5641 aaggtaagtc aggaagaaat cttaaattaa cgattgatct taattaccaa aacaaagtag
     5701 aaagtatctt gaaacaatac tatggcagtg aattatctag tgggcgcgct agtttttccg
     5761 aagggatgta tgcagttgct atagaacctt caactggtaa agtgttagca atggctgggc
     5821 tgaaaaatga tcatgggaat ttagttgatg acagcctagg cactattgct aagaatttca
     5881 cgccaggatc tgttgtgaaa ggtgcgacgc tatcatcagg atgggaaaat aaagttctta
     5941 gaggaaatga agtgctttat gaccaagaaa ttgctaatat acggtcatgg tttacacgag
     6001 gtttgactcc aatatctgcg gcacaagcgc tagagtattc atctaatact tatatggttc
     6061 aagtagcact tcgtttaatg ggacaagact ataatacagg agatgcttta actgatcgag
     6121 gctatcaaga agcaatggct aaactaagga aaacttacgg cgaatatggt ttaggggttt
     6181 ctacaggatt agatttacct gaatcagaag gttatgtacc tggaaaatac agcttaggaa
     6241 caactctaat ggaatcgttc ggtcagtatg atgcctatac accaatgcaa cttggtcagt
     6301 atatctcaac tattgcgaat aatgggaatc gtttagcacc tcacgtggtt tcagatatct
     6361 atgaagggaa tgattctaat aagttcgctc aattggttcg ttcaatcact cctaaaacac
     6421 taaataagat agctatctca gatcaagagt tagccattat tcaagaaggt ttttataacg
     6481 ttgtcaatag tggaagtggc tatgcaactg gtacgtcaat gagggggaat gtgacaacca
     6541 ttagtggtaa aactggtacc gctgaaacat ttgctaaaaa tataaatgga caaacagttt
     6601 ctacctacaa cttaaacgct attgcctacg atactaatcg taaaatagca gtagcggtaa
     6661 tgtatccgca tgttacaact gatacaacaa aatcccatca attagttgca cgagatatga
     6721 ttgatcaata tatttcacag ttcacaggac aataagagag gacatttgaa tgctttaccc
     6781 aacaccaatt gctaaattga ttgacagctt ttcaaaatta ccgggtatag gtactaaaac
     6841 agcaacacgt ttagcttttt acaccattgg tatgagtgat gaagatgtta atgaatttgc
     6901 taagaattta ttagctgcta agcgggaatt aacctattgt tctgtttgtg gtaatttgac
     6961 tgatgatgat ccttgcttaa tttgtacaga taagacgcgt gaccagtctg ttatcttggt
     7021 tgttgaagat agtaaagatg tgtcagctat ggaaaaaatt caagagtata atggtttgta
     7081 tcatgtctta catggcttaa tttcacctat gaatggtatt agccctgatg acattaattt
     7141 gaaaagttta attactcgat tgatggatgg tcaggtgaca gaagttattg ttgctaccaa
     7201 tgcgactgct gatggtgaag ctacatcaat gtatatttca cgagtcttaa aaccagctgg
     7261 tatcaaagtg acacgattag cgagaggttt agctgttggt tcagatatcg aatatgcaga
     7321 tgaagtaacc ttgcttcgag caatcgaaaa tcgtacagag ttataattgt tataaagaaa
     7381 aggttgggaa tctcctaact ttttctttta caatactaac tttaagtgga ttgatttttg
     7441 aaagttaagt taaaatatgg tattattaaa aggtcaatct tagattagat agaaaagaga
     7501 agaaatatgt caaaagaaac attgatcttg ctttacggtg gtcgctctgc ggaacgtgaa
     7561 gtatctgtac tgtctgcaga aagcgtcatg cgtgctatta attatgataa attttttgtt
     7621 aaaacttatt ttatcacgca agtaggtcaa tttattaaaa cacaagaatt tgatgaaatg
     7681 ccatcttcag atgaaaagtt aatgacaaac caaactgttg atttagacaa aatggttcgt
     7741 ccaagtgata tctatgatga taatgcaatt gttttccccg ttttacatgg accaatgggg
     7801 gaagatggtt ctatccaagg atttttagaa gttttaagga tgccttatgt tgggactaat
     7861 attctatctt caagcgtggc tatggataaa attacaacaa aacaagtcct tgcaacagta
     7921 ggtgtacctc aggttgcata tcaaacttat tttgagggtg atgatttgga acatgcgatt
     7981 aaactctctt tagaaacttt aagtttccca atttttgtaa aaccggctaa tatggggtca
     8041 tcagtaggta tttcaaaagc gacagatgaa tcctcacttc gctctgcaat tgacttagct
     8101 ctcaagtatg atagccgtat tttgattgaa caaggcgtga cagctcgtga aattgaagta
     8161 ggtattttag gcaataatga tgttaagaca acttttcctg gcgaagttgt taaagacgtc
     8221 gatttctatg actatgacgc caaatatatt gataataaaa ttactatgga tattccagct
     8281 aaagttgatg aagcaactat ggaagcaatg cgtcaatatg caagtaaagc ttttaaagca
     8341 atcggggctt gtggtttatc acgctgtgat ttctttttga cgaaagatgg acaaatcttc
     8401 ttaaacgaac tgaatacaat gcccggtttt actcagtggt caatgtatcc tctgctttgg
     8461 gaaaatatgg ggctaactta tagtgatttg attgaaaagt tagttatgtt agctaaagaa
     8521 atgtttgaaa aacgtgagag tcatttaatt taaaaaagtt caactaagtc tttcttagtt
     8581 gagctttttc aaatctatga tatgttatga aagtgtgtta taatttagga gtatattcgt
     8641 ttataaggga tagcatacta tctacaacat tgttgctaaa taatagaaaa ggaaatgata
     8701 tgaagcttag tttgcatgaa gttgctaaag tagttggcgc taaaaatcaa gtatcagagt
     8761 ttgaagacgt tcccctggga aatattgaat ttgatagtcg taatatcagt gaaggtgact
     8821 tatttcttcc cttaaaagga gcaagagatg gacatgagtt tatagagatg gcttttgata
     8881 atggagcaat agcaaccatt tcagagaagg aaatagaggg tcatccttat cttttagtgt
     8941 ctgatgcact taaagcattt caagtcttgg cacagtatta tattgaaaag atgaacgttg
     9001 atgttattgc agtaactggc tcaaatggta agacaacaac taaagatatg atagctgcaa
     9061 tcttatcaac cacatataag acttataaaa cacaaggaaa ctacaataac gagattggct
     9121 taccatatac tgtgctacac atgccagaag atactgaaaa aattgttttg gagatgggac
     9181 aggatcatct tggagatatc catgtgttat ctgaaattgc aaaaccacgt attgctgttg
     9241 ttactttgat tggtgaagca cacttagaat tttttggtag tcgagaaaag attgcagaag
     9301 ggaaaatgca aatcacagat ggtatgagtt cagatggcat attaattgca ccgggagatc
     9361 caattattga tccatatctt ccagcaaacc agatgactat tcgttttggc catgaccaag
     9421 aattgcaagt gacagaactc aaagaagaaa aacattcctt aacttttaaa actaatgctt
     9481 tggagcacca attacgtata ccagtacctg gtaagtacaa tgcaactaat gcgatggtcg
     9541 ctgcctatgt tggaaaatta ttagcagtag cagaagaaga tatagtggat gctctagaaa
     9601 atcttcaact aactcgtaat cgcaccgaat ggaagaaatc agccaatgga gcagatattt
     9661 tatcagatgt ttacaatgct aacccaacgg ctatgcgatt gatactagaa accttttcag
     9721 ctattcccaa taatgatggt ggaaagaaga ttgctctttt agctgatatg aaagaactgg
     9781 gagaacaatc tgtggattta cataatcaaa tgattatgag tatcagacct gactctattg
     9841 atactttgat ttgttatggc caggatattg aggggttagc tcaattagca agccagatgt
     9901 tcccaattgg taaagtgtat ttctttaaaa agaatcaaga agtggatcag tttgatcaat
     9961 tgcttgcaaa agtaaaagat actcttaagg agaaagatca aatattatta aaaggttcta
    10021 actcaatgaa tctttctaaa atagttgata ttcttgaagt aggataatag aggggttagg
    10081 gtaacaagtt accctaactt ttttgttttt taatcacgaa taatattcag ttttattgag
    10141 gaaaatagat atgctaagca gaaaactggt gatatattca gttttttgat aatctgttta
    10201 ttgcgaacta tatttttaaa aataaaactt aagttcgttt ttaggaggat tatttgaaaa
    10261 atttaaatcg ttatgtagtt gcggtttctg gagtcgtttt acatttaatg ctaggatcaa
    10321 cttatgcttg gagtgtgttt cgtaacccaa ttatctcaga gactggttgg gatatttcat
    10381 cagtttcatt cgcttttagt ttggctattt tttgtctagg aatgtctgca gcttttatgg
    10441 gacacttagt agagcgtttt ggtcctagga taatgggaat gatttctgct attttatatg
    10501 gagcagggaa tgtgttaaca ggcttagcca ttgaaactca gcagttatgg ttactgtatg
    10561 ttgcatacgg tattttagga ggaatcggac ttggttcagg ttatattact ccagtatcga
    10621 ctattattaa atggtttcct gataggaggg gactagcaac aggattcgct attatgggat
    10681 ttggctttgc ttctttagta acaagtccgc ttgcacaatc cttactgatt aggattggtg
    10741 tgggtaaaac gttttatatt ttgggattag tatatttttt tgtcatgatg attgcctcac
    10801 aatttattaa acaaccacct caggaaaaaa taactatttt gactcacgat ggtaaaaaga
    10861 atgctatgaa ttcacaaatt atcactggat taaaagcaaa cgtcgctata aaatcaaaaa
    10921 ccttttacat catttggttg accttgttta ttaatatttc gtgtggctta ggtttaatat
    10981 cagcagcttc accaatggca caagatttag caggctattc cgcagaatct gcagccttat
    11041 tagtaggggt actagggata tttaacggtt ttggacgtct gttatgggca agtctctctg
    11101 actacattgg acgcccgttg acctttataa tattatttat tgtgaacttt attatgactt
    11161 ctagtttatt tttgtcattc aatgctattg tatttgcaat agcgatgtct attttaatga
    11221 cttgttatgg tgcaggtttt tccttattac ctgcttatct aagtgatatt tttggaacaa
    11281 aggaattagc tactttacat ggttatagtt taacagcatg ggcaatagca ggtctgtttg
    11341 ggcccctatt gttatcaaag acatattcat ggggaaattc ctatcaattg acattaatgg
    11401 tttttggttt tttattctta ttcggattat tgttatctct atatttaaga aaattaacaa
    11461 ctaaagttgt gtagtatata tctaatttaa aattctttgg ttttaccaaa gagttttttc
    11521 tttgaaaaat agttttgtct tatagcaaat aatttgatat actaagtatc tagtaaaaaa
    11581 atgatagaaa gataatacta aaatgataga attcttttct aatatcagaa cagagattcc
    11641 gcagatgcct ttacttatcc atagtttgat tttatctgtc ttaccttttc tgatgtggct
    11701 gactttggtt aatagagata agcctttgta taaaactatt tggagtatcc ttttaggact
    11761 tcagttaatt acgatttata cttggttttt ctgggcaaaa ttgcctttat ctgaaagtct
    11821 tcccctttac cattgtcgaa taggcatgtt tgtcgttctc ttagcgaggc ctagaatatt
    11881 aaaggattat ttcgcattgt taggagtagt aggtggagta ttggcaatga ttcacccaga
    11941 cttttaccct taccagtttc tacatgtgac taatattttc ttctttattg gacattttgc
    12001 tttatttgtc cttagtttac tacatctgat gacacagtcc aatttggaca aattaaatcc
    12061 aaaattgatt atacaactaa cgcttctcat taatatgtct ttgattttta ttaacctctt
    12121 gactggggga aattatggct ttatgatgaa gacaccaata ctaggaataa caaatccctt
    12181 tttaaacctt ttgatagtga caactctatt aagttttctc gtcctatttg ttaaacaaat
    12241 tttccaaaaa gtgagaatga gagctagttt taaaacagaa gaatcttaat tattttaata
    12301 ttattatatt atctagctat taaaaaataa atagctggat aatgtttttc atttttagct
    12361 atttttatga ttgaccgcta attattaatc tgctaagata tagtacgtaa taaaaactta
    12421 accggttaat tattttttga aatatatttt ttctggagga aaatagtaat gaataaatca
    12481 ttcaatacca aattaggttt ggtaactgta gcggcattat caggtattgt attaacgtct
    12541 caattacctg tgaacgctaa agctgagaca ccagtaatgg cagcaagtgc acaacaaggt
    12601 ttccgttttg tagcaaccgt tgtagatagt cagacgcgtg tcttgccggg aaaattggta
    12661 actttgtcag aggtgacaac tggtcagcct aaaattattg caagtgtaaa aacgaatgat
    12721 gccggacaag ctatttttaa taatctccct ataaaacgta accttagtgt gtctgttgat
    12781 ggacaagtaa aaggatatac tattcgaact gacgtagcag gctctagtaa agcagctagt
    12841 tttacagcaa caggagtagg gacaaatgag ccaacgtatt caaaaaaaac catagacatt
    12901 acagtgagag accaaaatgc tgaacctgtt tcaggcagga cagttacctt aaaaacacat
    12961 gctggtcgtg agattgctag cttggtttct ggtgacaatg gattaactcg ctttactgat
    13021 cgtttactag atggaacatt ctaccaatat tttgttgatg ggaaaaaatt ggtgatattg
    13081 tacctggaga atcaagaagt gcatatgtta atgttgcacc taaaaaagat tattttacct
    13141 ttacagtcac tgctttagat aaagatggac ttgttgttaa gggaaaagaa gtaacgttga
    13201 ctgatattac tgatggtaaa gcagtagcgc ttgcttcttt aaaaacgaat gataatggac
    13261 aagctatctt taccaaccta ccattatcaa gaaatatcag tgtttcaatt gatggaaaat
    13321 caaagggcta tactcttcga actgatgtag caggctctca taaagcggct gcattctatg
    13381 tagatggtaa aggtaccaaa gcacctcaat ttagtgcaga agcagcggtt gtaactattt
    13441 acgatgctaa tggaaatact atagcaaacc aagaagtgac attaactaat tcaaatggga
    13501 caattgttgc aaaaggtttg actgaaaaag atggcaaagc aaggttcgcc aataaattaa
    13561 tgggaggaac gttatacaat atttttgtaa atggtattga gatgcctaaa actgcccttg
    13621 ttggtagtga tgtcagtgtt tttctaacag ataaacaaat taagaaagaa aaacctgtta
    13681 ctcctgataa attggataaa gaaatgaacc aagagaagga aaaggagctc ttagatactc
    13741 caaaaccagt accacctact tcgaaaccaa cagcaccaat taaaaataaa aaggacgatt
    13801 cccctaagaa gtttacgaaa agtcaacctt ctagtttgaa gtcaagtgtt aaggctatag
    13861 gtgctaagcc aaaagctatg acagccttag ctaaaaaatt gcctaaaact ggtgatcaag
    13921 ccattagtat tttaactttg gttggtttta ttgtaacagg gttagcgatt atgttaggct
    13981 tctttaaaaa atcacgatca aaataattta ttttagaagc ttgggtcttt agacacaggc
    14041 ttctcttctt tcttcggaaa tagattgaac ttgaagaatg ttgttaaatt cgctataata
    14101 ataaagctat agacaagatt attgtttcag ttgagactac tgaaaataat actaagaaat
    14161 ggataaaagc taaggattga atatgacatt acaagacgaa atcaaaaaac gccgtacttt
    14221 tgccatcatc tctcacccgg atgctggtaa gacgactatt actgagcaat tattatattt
    14281 tggtggtgaa attagagaag cagggacagt aaaagggaaa aaatcaggta cttttgcaaa
    14341 gtccgactgg atggatattg aaaagcaacg gggtatctct gttacttcat ctgttatgca
    14401 atttgattac gcgggtaaac gtgttaatat tttggataca cctggacatg aggatttctc
    14461 ggaagataca taccggacct taatggctgt ggatgcagct gtcatggttg tagattcggc
    14521 taaaggtatt gaggctcaaa ctaaaaaact ttttgaagtt gttaagcatc gtaatattcc
    14581 ggtttttacg tttattaata aattggaccg tgatggacgt gagccactgg atttgcttga
    14641 agagttagaa gaagtactag gtattgcttc ttatccaatg aattggccaa ttggtatggg
    14701 gaaatcattt gaagggcttt atgatttaca taataaacgt ttagaactct ataaaggaga
    14761 cgagcgtttt gcaagtattg aagacggtga tcagctgttt gccaataatc cattttatga
    14821 acaagtcaaa gaagatattg aacttcttca agaagcaggt aatgactttt cagaacaagc
    14881 tattcttgat ggtgacctaa ctccagtatt ctttggctct gcccttacga actttggagt
    14941 tcagactttt cttgatactt tcttggagtt tgctccagaa cctcatggtc acaagactac
    15001 agaaggtaat gttattgacc ccttagctaa agatttttca ggttttgtct ttaaaattca
    15061 agctaacatg gacccacgtc atcgcgaccg tattgctttt gttcgtattg tttctggtga
    15121 atttgagcgc ggcatgggag ttaatttgac acgcactggt aaaggcgcta agctctccaa
    15181 tgttactcaa tttatggcag aatcacgaga aaatgtgacg aatgctgttg ctggggatat
    15241 tatcggggtt tatgatacag gaacttatca agttggggat accttaacgg ttgggaaaaa
    15301 taaatttgaa tttgaaccat taccaacttt taccccagaa ttatttatga aagtatcagc
    15361 aaaaaatgtt atgaagcaga aatcattcca caagggtatt gagcaattag tacaagaagg
    15421 agcaatccag ctttataaga attatcagac aggcgaatac atgctgggtg ctgtgggtca
    15481 acttcagttt gaagttttta aacatcgtat ggaaggtgaa tacaatgctg aagttgttat
    15541 gacaccaatg ggtaaaaaga ctgtacgttg gattaactca gacgatttag acgaacgcat
    15601 gtcctcaagc cgaaatattt tagcaaaaga tcgttttgac caaccagttt tcctatttga
    15661 aaatgatttt gcacttcgct ggtttgctga taagtatcct gatgtgaagc ttgaagaaaa
    15721 aatgtaaaat ttaaagtcac tcaaatttga gtgacttttt aagtttttta gactaaaata
    15781 gttataatac atagaaaaga ttggagatta acatgggaat tttttcggga ttaatgggaa
    15841 atgcttcaca agtggatacc gataaagtcg agaatcagtt atctgatatt ctgattccag
    15901 gtgaacaggt tgatttagct tatactttaa ttcgtgattt gattgttttt actaattatc
    15961 gtttaatttt agtcgataaa cagggggtga caggtaaaaa ggtttcttat aattctatac
    16021 cttatgcttc tatatcacgt tttacagtgg aaactagtgg tcattttgat ttagatgctg
    16081 agttaaagat ttggatatca tcagcaatag aaccagcaga agtacttcag tttaagaacg
    16141 accgtaacat agtgagtata cagaaagctt tggcgatagc tgttttaaaa taaacaattt
    16201 agcaagtgct gctactaaga gttatttgtt ttaattataa cttttaatag tgctaaattc
    16261 tcattttcaa aataaaagta gtgattttta tcactgcttt tattttgaag tatagtaggc
    16321 ctcaagcata tattttgaaa acgctgtatt tttttgataa gatatttttt tgaggagtat
    16381 taaataatga taatagaatt aaagagtgtt gggaaaattt ttccaaaatc acagtttaga
    16441 ttagatgata tttcttttaa tatagcagaa ggcgagatag ttggtctgat tggttctaat
    16501 ggtactggaa agagtactat tttgaagatg attaatggct taatccctta tgataaagga
    16561 aatatttatt atcaaggaaa agaagtgaag tctttttctg ataataagtt acgtcagatg
    16621 cgtaaagata ttgcttatat ttttcaaaat cataatttgt tagcaggaga aagtgtatat
    16681 tatcatctag ctttagttta taaattaaat catcaaaaag taaatcatga cgcaattaat
    16741 gatatcttag attttttagg tttgatggat ttaaaacagg tgaaatgcca tagtttgagt
    16801 ggtgggcagc aacaaaaagt tgcaatcgct atggctgttt tgcaaaaacc gaaacttatt
    16861 ctatgtgatg agatcagctc tgccttagat actaatagtg aaaaagagat atttaacttg
    16921 ttgtctgatc ttcgtgaaaa atatggtatc tctatcttga tgattgctca tcacctatct
    16981 cttttaaaac aatattgtga tcgtgtaatg attttggatc accagactat tgtagatact
    17041 gttgttccag tgaaagcaac tctgaatcaa ttaaagtcaa actacgtaga tcaagtaaag
    17101 gagtttttgt tacatgattg acattattga aaaacatggc gaaggtatct taaaatcatt
    17161 ttgggaaacc aatttaatgt tagggttaac cttgattctt tgttttctaa ttgcttttcc
    17221 aacaggtatt ttattgtttt cattgagaaa gtcttacttg attaaacatt ctttagccta
    17281 tcaactacta aatttatttt taggtacgtt acggagtgtc cctttcctta tttttatttt
    17341 tatcttaatc cctctcaatc gattaatttt tgggacctca tttggaacga tagcggcaat
    17401 cttacctctt actttggttt cagtcagttt gtatgcacgt tatgtggaac aagctttatt
    17461 aaatatacct caagttgttg tagatcgagc tctcagtcta ggggctaaca agagacagat
    17521 aatttattat tttttaatcc cctccatcaa gattgacctt gttttatcat ttactgcaac
    17581 ggctataagt attcttggct actcgacaat aatgggagtt attggtgcag gaggtttggg
    17641 tgaatatgcc tatcgttttg gctatcaaga gtatgactac ccagttatgt acttgattgt
    17701 tgtcttattt ataatatatg tgtttatctt acagagtttg ggctatttta tagcaaaccg
    17761 ctactcaaga aaataaaaga aaagaggaac tatgaaaaaa ttactttccc taacatgtct
    17821 aatcatgatg tctttatgtt tagtggcatg tactaagcaa gcaatgtcgt ctaagcaaat
    17881 taaagataag aatagtaaag aaaaggtgat tactgttgca acttacagca aacctacatc
    17941 taccttttta gatttgatta aagataatgt aaaagaaaaa ggatatactt taaaggttgt
    18001 catggtctct gactatattc aggctaacat tgctttagaa aacaaagaac atgatgctaa
    18061 ccttttacaa catgaatttt tcatgagtat ctttaataag gaaaatgatg gtcatctagt
    18121 gtcaattaca ccaatttatc attcattggc tggtttttat ggtcaacatt tgaaaaatat
    18181 tgccgagctt aaagacggtg ctaaggtagc gattccgtct gatcctgcca atatgactag
    18241 agctctgcta ttattgcaag aaaagaaact tatcacctta aagaatacgt ccaaaaagac
    18301 caaggctatc gaagatatta ttactaaccc taaaaaatta cgaattgaac ctgtagcatt
    18361 acttaacctc aatcaggcct attttgaata tgaccttgtc tttaatttcc ctggatatgt
    18421 gacaaaaatc aatctagttc ctaaaaggga tagattatta tatgagaaaa aaccagatat
    18481 ccgttttgca ggtgccttgg tagctcgtga agataataaa aatagtgata aaataaaagt
    18541 acttaaagaa gtactaacaa gtaaagagat tcgtcactat atcactaagg agattccaag
    18601 tgaagcagac gttgcgttct agatagataa actatgaacc ctccttcttt atagatggag
    18661 ggtttttatt gccttttttc tattttctga agcaactaat agaactagca atttatgaaa
    18721 gcgtgttatt tattagattg accttatttt atatcttttt acttttaatt gtgttacact
    18781 aagtaggttg tcagaaaaga taactaataa aggttcataa ggcagcttcg cactgtcttt
    18841 tagaaaagag aaacatttga aatttacaga acttaactta tcgcaagata ttctttcagc
    18901 ggtagaaaaa gcaggctttg tagagccatc accaatccaa gaaatgacta tcccacttgc
    18961 tcttgaaggc aaagatgtca ttggtcaagc gcagacaggt acaggaaaaa ctgcagcttt
    19021 tgggttacca actttaaata aaattcatac tgaagataat actattcaag cacttattat
    19081 tgctcctaca cgtgaacttg cagtacaatc acaagaagaa ttgttccgtt ttggacgcga
    19141 taaaggtgtt aaggtccgct ctgtttatgg tgggtcaagt attgaaaaac aaatcaaggc
    19201 attacgttca ggagcacacg ttgttgttgg aactccaggt cgattacttg atttaattaa
    19261 acgtaaagca cttaaactta atcacatcga aactcttatt cttgatgaag cagacgaaat
    19321 gcttaacatg ggatttttag aggacattga agcaatcatt agtcgtgtac cagaagcacg
    19381 tcaaactctt ttattctcag cgacaatgcc tgatccaatc aaacgtatcg gggttaaatt
    19441 catgaaagat cctgagcatg ttaaaattaa agcaactgaa ttaactaatg tgaatgttga
    19501 tcagtattat gttcgcgtta aggaaaatga aaaattcgat actatgacac gtttgatgga
    19561 cgttgatcaa ccagaattgt caattgtctt tggacgtacg aaacgtcgtg tagatgaatt
    19621 aactcgtggt ctaaaattac gtggtttccg agcagaagga attcatggag acttagatca
    19681 aaataaacgt cttcgtgtta tcagagactt taaaaatgac catattgata ttcttgttgc
    19741 cacagatgtt gctgcgcgtg gtttagatat ttcaggcgtt acacatgttt acaactatga
    19801 tattccccaa gatccagaaa gttatgttca ccgtattgga cgtactggtc gtgctggtaa
    19861 atctggtcaa tctatcactt ttgtttcacc aaacgaaatg ggatatttga caattattga
    19921 aaacttaact aaaaaacgta tgactggaat gaagcctgct actgcaagcg aagcatttca
    19981 ggctaagaaa aaagttgcat tgaaacgtat tgcacgtgac tttgaggatc aagaattagt
    20041 ttctaaattt gataaattta aagccgatgc tttagaatta gcaactcaat atacaccaga
    20101 agaacttgct ctttatgtct taagcttaac agttcaggat ccagaatcgt taccagaagt
    20161 tgaaatcaca cgtgaaaaac cattaccatt taaaccatca ggtggtggct ttaaaggtaa
    20221 aggtggacga ggtaatggcc gtggtggcga tcgtcgacgc aatgatcgtg gtgatcgtcg
    20281 tggaaatcgt gaccgtgatg atcgtggagg tcgtcgtgat tttaaacgtc gtgatgataa
    20341 gttcaagaaa gataatcgtc gtcaagaaaa taaaaaacct cataaaaaca cttcaagtga
    20401 aaaacaaact ggatttgtta ttcgcaacaa aggcgataaa taagaggatt acgaaaaagg
    20461 agttgctgag cgactccttt tttatttttt aagataaagc gtagatagtg atatccgtaa
    20521 aattgcctta taactttaag aatgaagaca aaagattaaa gtgaggttga aatttaaaag
    20581 ttaaagaagc gaggacaggc tcattgcact tagtatatta tcgtattatt gttcattttt
    20641 atgttgttta atataagtca attttgcttt ccgagtcttt tgtttaaata atgcttctgc
    20701 cctcattgct tcttgtttgc tattaaaagc ttcagaataa agaagtttta ctggcaatct
    20761 cgcacgagta tatttagcac cttttccggt attgtgtgta ttgagacggc gcttgacatc
    20821 tgtagtataa cctgtataaa gtgttccatc tgaacattct aaaacataca tataagcagg
    20881 aactttaatc atgagatttt ccgttttccc cgaaatatat atcaaaaata gtctcagtat
    20941 agtctccatt atcttgatga acaacaagag gaggaagtat tttcatacct tctgtcgagc
    21001 catctttaat agcttcaatt aaaagcatat tagcatcctt tcctaatttt ggataaacaa
    21061 attggatacg tttaggtgct aaattgaact gatgcatcgt atcaataatt tcaaggaaac
    21121 ggtctggacg atgtaccata gcgatacgtc cttttgtttt tagagcatgt tgagcaattt
    21181 gacaaatctc tctaaggttt gttgttattt catgtcttgc aaggaggtaa tgcgggctta
    21241 gatttttttt agaggtttcg cttgctttga aataaggtgg attgcaaagc attaaatcaa
    21301 cgtttgaacg ttgaacatga tcaagaagat tttttaaatc atcattaatc atagtaacct
    21361 gtttttccaa tttattgagc ttaattgaac gcttagccat atccgcaagg cgctcttgta
    21421 gttcgatctc tataattgtc gcacttgttt tggtgctggc aaacaaaccg acagcaccat
    21481 taccactgca taagtcaacg ataagaccac gagaaggaag tttagggaac cgagacaata
    21541 aaacactatc tatggagtag gaaaaaactt ctttgttttg gatgattttg acatcagttg
    21601 aaaaaagctg atcaatgcgt tcattttctt ttaaaattgt atctatcata ggcttattat
    21661 agcaaaatta cagcttccta gcactagttt tgaaagtgtg gaggttgatt ttaagataat
    21721 tctttctaaa aaaaaggaag catgataaaa tagaagttac ttgatgaaag gtagaggaga
    21781 aacatgttct atacttactt acgtacttta gttatgttct tgatttgggt ggctaatggt
    21841 aatgcgcact atcataatga agataaaatg ttaaaagatg atgaaaatta tattttagta
    21901 gctccacaca gaacattttg ggatcctgtg tacatggctt ttgctgctag acccaaacag
    21961 tttgttttta tggccaaaaa agaattattc acgaatcgtt tgtttggttg gtggattaaa
    22021 atgtgcggtg ctttccctat tgatcgtgaa aagccagggc aagacgctat tcgatatcct
    22081 gtaaagatgc tgaaaaatag taaccgttct ttggtgatgt ttccgagtgg aagccgccac
    22141 tccaaagatg tcaaaggagg cgtagctgtt attgcaaaaa tggcaaaagt cagaattatg
    22201 ccagcagcat atagaggtcc tatggttttt aaaaatctat taaaggggca tcgtgttgat
    22261 atgaattttg gtaaccctat tgatgtttct gatattaagc gtatggatgc cgaagggatt
    22321 gcagaagtat ctcgtcgtat tcaggaagaa tttgatcgtt tagatagaga aaatgaaact
    22381 tatgatgatg gtaagaaatt aaatccattg acttatattt atcgtctacc tttagcaatt
    22441 atagctattg tcttgcttgt cctaactcta atttttagtt atttagcaag ctttgtgtgg
    22501 gatccacaaa aacatctaaa ataagggtta catggaaact atttactttt tagtaaatag
    22561 ttttttttgt ttttaatagg aaaatctctt tagttacgaa taagtaaatg gaggaatatt
    22621 tttatgtttg aaatagtatt ggaaaaaatt aaatcgcata aatgggagac aacaggaatt
    22681 attgtaggac tcttattgtt tggaattttg ggtttaaatc attttggcac tcatcataaa
    22741 gaagacgatt taaatatcaa tttagagaaa aaagtatcaa ctataacaga aaaaaaggta
    22801 ccaatgatta gtcacgtcaa agataaagta tctaatcaag taacggttga cgtaaaaggg
    22861 gcagtcaatc atccaggtgt ttatagtttg ccttctcaaa gtcgtgtaac agatgcgatt
    22921 aagcgtgcag gtggactaag caatctcgct gatagtaaat ccgttaatct agcacagaaa
    22981 ttacaagatg agacggttat ctatgttgct caaaaaggtg aaaaaataac tgttgtggaa
    23041 gaggagaaag caaataatat tgctactcag ggaaatagta aggggaaaat taatcttaat
    23101 aaagcagatt tatcaagtct tcagaccata tcaggcgttg gagctaaaag agctcaagat
    23161 attttagatt atcgtgatag tcaaggtggt tttaaaacta ttgatgacct caaaaatgta
    23221 tcaggtattg gtgagaaaac actagaaaaa ttacgtcaag atgttacact tgactaagta
    23281 ttttcctcta aaacctattt atttagcatt gttggtcttc caaatttact tactagtgtt
    23341 ttcttggaca atgcttggtt gtgcctttct tttatttact tttatttttc tgatttatca
    23401 atatgatcgt gaaactattt ttaaaacaat aacaatagta atttttttct tattttattt
    23461 tttatggcaa aatcacaata tgaatgtcca atatcaaaga gtaccgaatc atattagcca
    23521 gattaaagtg cgtattgata ctatttctat caatggtgat gttttatcat tccaggcaga
    23581 tgcttcaggt aacacttatc aagcttttta cacattaaaa aataaaagtg agaaagatta
    23641 ttttcaaaat cttgataata atataatgat aattgcagat atcaaacttg aagaagcaga
    23701 ggagagaagg cattttaatg gctttgatta tcgtcagtat ttaaaaagac atggaattta
    23761 tcgtatcgcc aaagtgacaa agataaaaca gatacgctta tttcaacata ggtctttctt
    23821 tgatcttatg tttaagtggc gtagaagtgc aattgttatt agtcaaactt ttccaaatcc
    23881 tatgcgtcac tatatgtcag ggcttttgtt tggatatcta gataagacct ttgatgacat
    23941 gtccgattta tatagtagtc taggtattat acatttattt gctttgtcag gtatgcaagt
    24001 aggttttttt ctcggtattt ttcgttatat ctgtctacgt attggcttac gtctagacca
    24061 tgtttggtta cttcaaatac cattctcgct aatttatgct ggtttaacag gctttagtat
    24121 ctcagtcgtt agggcactta ttcaatcttt attatcacat agcggtgtca agaaagatga
    24181 gaactttgct ctctgcttgt taatttgtct tatctctctc ccccactcac ttttgactac
    24241 gggaggagtt cttagctttg cttatgcttt tatacttacg atgacctcct ttgatcattt
    24301 ttcgagtata aaaaaagtag ctatcgaatc tttgacagtc tctgtaggaa ttcttcccat
    24361 actaacctac tatttttcgg gttttcaacc aatatcaatt atattaacag cacttttatc
    24421 ttttgcattt gatattatat ttttgccttt attaactgtt atatttgtct tatcaccgtt
    24481 cgttaaatta agttgtatta atagtttgtt tgaaatccta gaagtattat taaaatggac
    24541 tgggcaactg tttccaagac cacttatttt tggaaagccc agcctttttc ttttaatagt
    24601 catgattata attttgggat tactttatga ttattatcat tctaaatgtt ttcgttattg
    24661 ctcccttctt attatcttta ccttgttttt tatcactaag aatccaatta ctaacgaggt
    24721 tgcgatttta gatgttggac aaggagatag tattttagtg agggattggt taggaaaaac
    24781 aattttaatt gatactgggg gaagggtgag atttgaacag cctgaagaat ggaaacaaaa
    24841 agtaaaccag tctaatgctg agagaacgct cattccttac ttgaaaagca gaggtattag
    24901 caagatagat aatttagtga taactcatac cgatacagat catatggggg atatggaagt
    24961 tatctcaaag cattttaaag ttgcacgttt gattacaagt tcaggttctt taacgaattc
    25021 gcagtacgtt aagcgtttat caaagatagg agtagctgta aaatctatag aagctggtga
    25081 taaactttct gtcatgggaa gttatttaca agtactttac ccatggcaca agggtgatgg
    25141 aaaaaataat gattcaattg ttttatatgg acatttatta ggaaaaggct tcttatttac
    25201 cggtgatttg gaggaagagg gagaaaagca gttattagaa gcttatcctc atttatcagt
    25261 agatatcctt aaagcaggac atcatggttc taagggctca tcaagtctat cctttctgaa
    25321 aaagttgtct cctagtgtgg ttctagtgtc agctggtaaa aataatcgtt accagcatcc
    25381 tcatcaagag actttacaaa ggttccaaaa gattaaaagc aagattttcc gaacggatca
    25441 atcaggtaca attaggctaa caggatggtg gaagtggcat attcagacag ttcgttgatt
    25501 tattaaaatg attgcgtatc tgttaaatac aatctatttt tcgcaaggat tcagtaattt
    25561 taagcttgac atggaacggg ttccagcatt tatactgatt tcagaagaag agaggtattt
    25621 tttatgacta tcaaacgtat tttttgtgac atggatggta cactattaaa tagtgaagga
    25681 caagtgagta aaagtaacgc taccttaata cgtgaggcag cgattccagt aactttagtg
    25741 tctgctaggg cacctatgga aatgaaggat gccgtagatg cacttcaatt aggaggagtc
    25801 caagttgctt ttaatggagg attgatttat agaataggag ataataatca agttctacct
    25861 attcatacac aaatcattaa aaaaagcaca gtaaaacaat tgctaagagg aatacggttc
    25921 cattttcctc aagttagttt atcttattac gatcttaata actggtattg tgataaaata
    25981 gatgaaggaa tccgatacga acatagttta actcaacaat gcccaacgtt tattcataat
    26041 gaggatcagt ttttagaagg tcacacaaat acttttaaaa ttatgatgat tactttcgat
    26101 gaagcaaata tgctagaatt agaaaaatat cttcaaagtt tggagttacc agaaatcact
    26161 attcaacgtt caggtaaagc gtatcttgaa attacacatc tcttagccaa aaaatctaaa
    26221 ggaattgctt acatccttca aaaagagcag ttagctagag aagaaacggc tgcatttgga
    26281 gatggacata atgatttgcc tatgcttgaa atggtaggtt accctattgt tatagataat
    26341 gcttttgatg atatcaaagc gatagcatat caactaacta agtctaatga tgaagacgga
    26401 gtgggatatg ggattcaaaa atttttaaag taggagcctt ataatgacaa gtattagtga
    26461 tatagctaaa aaggcaggtg ttgctaaatc aacggtttcc cgtgttatca atcatcaccc
    26521 acatgtttct gatgagacgc gacaaaaagt aatggctctt atcaccgaat tggattatat
    26581 accaaatcaa ctagcgcggg atttaagtcg aggcaaaaca caaaaaatag gtgtcgtcat
    26641 tccccacacg cgccatcctt attttacaca attaataaat ggcctattag atgctgctaa
    26701 aactaccgac tatcaacttg ttgtgatgcc ttcggattac aaccaagaat tagaactttc
    26761 ctacttgaaa cagctcaaga tggaagctat tgatgcttta atattcactt caagagcaat
    26821 tagcctcgac attattgaaa cttatgcaaa atatggacga atagtagttt gtgaaaaatt
    26881 gcaggaatat aatcatctat cctctgctta ccttgatcgc tattcttcat ttctagaggc
    26941 attttctgat atgaagttac gaggtttgga gcacttagtt ttattatttt caaggaataa
    27001 tgaatcctct gctacctatc aatccgcatt attagcttat caagaggtct atgggcaact
    27061 atcctctcca tatatggtag ttgggaatgt tcatgacttt aatgatggac taaatttatc
    27121 ttatcaattg gtaaaagaag ttagtataga tggtatatta gctacaagtg atgaagttgc
    27181 ggcagggcta ataaaaggtt atgaagaaag taggaaaaaa tgtccttata ttattggtca
    27241 ggaatgttta ttagttgggc aacttttgaa actcccaaca attgaccaca aatcctatta
    27301 tttagggaaa ttagctttta aacaggcttt agctgaaaag attagtcaag aagttttatt
    27361 ctcagagttt ttatctcaag ataattagtc attcaaaagt aatagaaaaa aggtttccaa
    27421 ctaaacttga ttggaaacct ttttatttaa tgattgataa ttttaagcat ttgtttgaaa
    27481 gcagtctctg cttcaggaat cggtaagact ggataaacgt ggttcatttt ttgaccaacg
    27541 atatagtggt gtttaatgtt ttcttttgct aatttttgat gtaataattg attatcgggg
    27601 tagaaaatat cgtgcgttcc tataaaaaat gatattggag ccatcttgtt aaattggcta
    27661 ctgtaaagag gactaacttg aggattttta aaattctttt ttccatttgc ccagtaagga
    27721 gctacttgtt gtaattgcca agctttaaga aggggatctt ttttttgaac tttttcaatc
    27781 tttggattat tagtagcaat atctaaccat ggtgatatta aaataatttc ttttggttgt
    27841 ttgatatgct ccgtgacaag gttatcagca atacctaaag caaggcctcc gcctgcactt
    27901 tcacctacta ggataatctg tttgtgagat gtgacgctag ctaatgtatt ttggtataat
    27961 tttttaattt tggggatagc atcactataa ttatatgtag gagctttagg gtaaatagga
    28021 aagacaactt ttgcatctaa ttttttagct agtttattga caaaaatata ttgtaattcg
    28081 gaagcttgat ggatatatga tcctccatga acatagaaga taaccttttg cttaggatta
    28141 tttttatcat tccaagtgaa tgtttgcatg ccatcagtag cagtaatttc taccttttca
    28201 ttatttaatg cctgaggaat gtctacagga agaacattac gtttagctac cttagttaag
    28261 taattttggt attctttaat ggtttgaggc ttaatctgat ttaccacagg tagagttttt
    28321 tcaatagtat tgctagttag ggatcttttt tcaacaatat gcgtgtaagc atatgctccc
    28381 ataaaaatgg caaaaaagat tagaagtagt agtacaataa gttttcgata gtttttcatg
    28441 attatccttt tgtaattgag ctcgtcatgc taaaaatgat tagttgttat tctataataa
    28501 agtagatacg aaaacaagaa aataatgcta taaaatttca acctattata attttttgct
    28561 gatagtacgt atgaaccttt aatttgctaa aatagaagag aggagagtgt tattatgttt
    28621 cacagagaag atttttcaaa agcattatta aaaaaagctt tgcagcccgg tatgagagtt
    28681 atggatattg gttgtggtag tggggaatta acgcgtttgg ctgctgatat cgttggtaaa
    28741 gaaggtgatg ttgtaggaat tgatattaat gagcaacttt tatggcaagc tagacatgat
    28801 aatggctatg ctaatgttag ttatcaaaat gcagatttag cagcactttc ctcctttgaa
    28861 acaaaaagtt ttgatgctat tattggacgt cgtgtattaa tgtatcaaaa aaatccaaag
    28921 aaagtaatag aaagtttaac acctttgtta aagcaaaatg gtttttatgt ttttcaagaa
    28981 agtgatgcaa tgtgtttaga actagctaag cctagctttg taaatatgaa taaaatacaa
    29041 tcactaatct ggcaaactgt tgagaaggaa ggaggacaca ttcatattgg aagtcaactt
    29101 gagcaattaa tgttagaagc aggcttaatc gttgaccatt ttgtatcaga aaatgtcatt
    29161 caaacgtcac aaactggtac agatctatct tggctactaa atatcatgaa agaaagaata
    29221 caaaaatata gtgaccttca ggggttagaa gatgtcaatt tacaaaaaga attagaagag
    29281 aataatgacc attttatccg agattgtcaa tttggaatta tttctcatag agttgatgaa
    29341 tgattttaag tattatttga caccttgaaa agtttcctta tggtataata aggaatatga
    29401 ttgcgattga agaaattggg agaattaccc cagataattt agggttagta actgttttag
    29461 caggagaaga tttagggcaa tatgctcaga tgaaagagaa gctatttcag gtaattggat
    29521 tcaataaaga cgatttagcc tattcttatt ttgatttaag tgaagaagaa tatcaaaatg
    29581 cggaattaga cttagaaagt ttaccttttt tatcagacta caaggttgtt atttttgatc
    29641 agtttcaaga tattacaact gataaaaaaa cttatttaga tgaacaagca atgaagcgct
    29701 tggaagccta cttgcagaat cctgtagata ctacgagact tgttatttgt gctccaggaa
    29761 aacttgatgg aaaacgtcga cttgtaaagt tattgaaacg tgatgctcgt gttttagagg
    29821 ctaatacttt aaaagaatca gatttaaaga cgtattttca aaaatatgct caccaagaag
    29881 gattagtttt tgaagcaggt gtttttgatg agttattgat taagtctaac tatgatttta
    29941 gtgatacttt aactaatatc gctttcttaa agtcatataa aacagacggt catatttcgt
    30001 ctaatgatgt tagagaggct atccctaaaa gcctgcaaga taatatattt gatttgactc
    30061 aagatgtctt acttggtagg atagatttag cgcgtgattt agtaagagat ttacgtctac
    30121 aaggcgaaga cgaaatcaaa ttgattgcaa tcatgttagg acagtttcgg atgtttttgc
    30181 aagttaaaat attggctagt aaaggaaagt cagagagtca aattgtttct gaactatccc
    30241 attacatagg acgtaaaatc aacccttatc aagtaaaatt tgctgtcaga gattctagaa
    30301 accttcccct tgctttttta aaagaagcga ttaggatttt aatcgagact gactatgcta
    30361 ttaaaagagg gacttatgat aaggattatc tctttgattt agccttatta aaaattgctc
    30421 ataaaaagcc ctaattaaag aaacaaaaga gttgaaagct gttccaaatt cctatagaat
    30481 aagggtaaca atagatgaaa agaggatttc gacatggcaa ttattttacc agatttaccc
    30541 tatgcatatg atgcgcttga gccacatatt gatgctgaga caatgacact acatcatgat
    30601 aagcaccatg caacttatgt tgctaatgca aatgctgctc ttgagaaaca tcctgaaatt
    30661 ggagaagact tagaggcgct cttagctgat gtttctcaaa ttccagaaga tattcgtcag
    30721 gcagtcatca ataacggtgg tggacatctt aaccacgctc ttttctggga attgatgtca
    30781 ccagaagaaa ctcaaatttc acaagagtta tctgaagaca ttgatgcaac ttttggttca
    30841 tttgaagact ttaaagctgc tttcacagca gcagcaacag gacgttttgg ttcaggttgg
    30901 gcttggcttg ttgttaatgc tgaaggcaaa cttgaagtgc tttcaactgc caatcaagat
    30961 actccaatta tggaaggtaa gaaacctatt ttaggacttg atgtatggga gcatgcttat
    31021 tacttaaact atcgtaatgt tcgtcctaac tacatcaaag ctttctttga aattattaac
    31081 tggaataaag taaatgaact ttaccaagct gctaaagctt aataaaaaaa cgagtcgata
    31141 ggctcgtttt tttattattc taaaagttga taatgttttt aaaagtctag aaatccaaac
    31201 tcagatagaa ctagaacagt cctattatct tcaattaagc gaataagttt attgaaagcg
    31261 cttgacaaga ttagtaagaa atgataaaat taagacgttt tcaggtgtta ttatttaagg
    31321 taagcaagct gataacagat aaaattgcat aataaaggat gttatcacat tttgtgagca
    31381 gggacctatt caacgacgac tttttgttgt gtcttggatg ggtcattttt tattgtgcga
    31441 gtgaaggact aggactttaa tgattataaa acgcgtatta aatcataatg cagtcatatc
    31501 agtaacacat caaggattag atgtgttact gatggggaaa ggaattgcat ttaagaaaag
    31561 aataggtgat aggattaata gcgatgctat tgagaagtca tttgttttaa aaaatagtga
    31621 taacatgaac cgttttacgg agttatttat tactgttcca gaagaagtag ttgcatgttc
    31681 agaacgaatt atcaacctag gtaaaatcaa attgggtaag aacttagatg aaattcttta
    31741 tatcaatttg acagatcata ttcattcggc tattgaaaga catgaacaaa gaatggttat
    31801 ccaaaatcca ctaaggttgg aaatccagcg ttactatcct gatgaatact ccattgggat
    31861 gaaggctctg gaacttataa aggatgaatt gggtatttgt ttaacaattg atgagtcagc
    31921 ttttattgct atgcactttg taaatgctgg attggataat ccgtttaatg aagcgcataa
    31981 aatcactgag attgtatctt acattgaaca gaaagtaaaa atagattttc gaacagaact
    32041 agatgaaagt tcaattgatt attatcgttt catgacacat accaaattat ttgctcaacg
    32101 cgttttatca ggaatgaaat acgaagatga tgatgctgat ttacttttgg tagttaagaa
    32161 aaaatatccg agagaatata aatgtgttaa agagatcggc aacaatatgg ctatccaata
    32221 ccaatatcaa cttaattcaa gtgaactcct atatctgacg gtgcatgtta aacgattagt
    32281 taaaaattta aaggagatat cagatgacta aataccaaga gactgctaaa gctattttag
    32341 cagcagtagg gggagaaaaa aatattcaac acgtaaccca ctgtgtgaca cgattacgat
    32401 tagttttaga caatgatgaa attgtcaacg atcaggttat taaaactatt ccgaatgtta
    32461 tcggtgttat gcgtaaaaat gatcaatacc aaattatttt gggaaatgat gtcaataatt
    32521 attataatgc ttttttggcc ttaggtcatt ttgaaaatac taccagagaa ttttcatcac
    32581 aaaaaaagag ttcaatattt gagaaattaa ttgaaacgat tgcaggagtc attaagccgc
    32641 ttatacctgc tcttctagga ggaggtatgc tcaaggttat aggtattttg ctccctatgc
    32701 tcggtataac aagctctagt tctcaaacag tagcttttat caattttttt ggtgatgccg
    32761 cttattattt tatgccaata atgattgctt attcagctgc gtcacgattt aaagttacac
    32821 ctgtattagc tgctacagta ggagggattc tcttacaccc tgcttttgtg acaatggtag
    32881 ctgaaggaaa accattatct ttctttggag ctccggttac actggctagt tatggttctt
    32941 ctgttattcc aatcttaatt atggtttttc ttatgcaata cattgagaga tggattaata
    33001 aaattgttcc tagtgtaatg aagagctttt tacaaccgac tttgattatt ctcatttctg
    33061 gctttttagc cttagtggtc gtggggccac tgggagtaat tattggtaaa ggattgtcta
    33121 gtgcaatgct ttcgatttac catgtggcac catggttagc tttagctatt cttggtgcta
    33181 ttatgccatt ggttgttatg acaggaatgc actgggcatt tgctccaatc tttttagctg
    33241 cttctgttac gacaccagat gttttgattt taccagcaat gttagcttct aatttagctc
    33301 aaggtgcagc ttcccttgct gttgcagtta aggctaaaca aaaacagaca cgtcaggtgg
    33361 cctttgcagc aggtttatca gcactactag ctggtatcac agaacctgca ctttatggtg
    33421 tcacactgaa attcaagaag cctttatacg ctgcaatgat atcaggtggt ttagtaggag
    33481 cttatattgg attagttaat attgcttcct atacatttgt agtgccatct attattggtt
    33541 tgccacaata catcaatcca caggggggca ataatttcag taatgctgtt attgtagcaa
    33601 tagctactat tattttgact ttcattatta catggttctt gggaattgat gaaggagaaa
    33661 acgaaaaaag tagtattaat gctcaagagt acacacatat tagaagtggt ttatcaaaaa
    33721 aagagacatt gtattcacct atggtaggca atgtattacc tttatctaaa gtacccgatg
    33781 aaacattttc atctaaatta ctaggtgaag gtttagcaat cactcctagt gtgggtgaag
    33841 tttatgcacc atttgatggt gaaattataa gtttatttcc tacaaaacat gccattgcct
    33901 taaaggatga taagggagtt gaagtgttaa tccatattgg aatagatact gtagaactaa
    33961 atggagaagg ttttgaacaa ttggttaaag ttggcgattt tgttaaacga ggacagttac
    34021 ttttgcgaat ggatattgat tttattagtt caaaggggta ttctcttatt agtccagttg
    34081 tagttacaaa ttcaattgac caacttgaaa ttatcgttaa agacgaagag acaatggtta
    34141 caaatgaaga tgatttactt gtaattcttt aaaaggaagg atgataatat gacgattttt
    34201 cctaaacatt ttctatgggg aggtgcagtt gctgccaatc aggtagaggg tgcatttaga
    34261 acagatggca aaggtttatc tgtacaagat gtacttccaa atggtggtct aggcgatttt
    34321 accgccaaac ctactccaga caatttgaag ctagaagcaa ttgattttta tcataactat
    34381 aaaaatgata taaaactgtt tgcagagatg ggctttaaag tttttagaac ctctattgcc
    34441 tggtctcgaa tttttccaaa tggtgatgat agtgctccaa acgaagctgg cttacaattt
    34501 tatgataatt tatttgacga gttgcttaaa tataatatcg agcctttggt taccttatct
    34561 cattatgaaa caccccttca tcttgcaaag acctataatg gttgggctga tagacgtttg
    34621 atagcatttt ttgagaaatt tgctcaaaca gtcatggagc gttataaaga taaggtaaaa
    34681 tattggttaa cttttaatga agtgaattct attttacata tgcctttcac cagtggtgct
    34741 attatggccg acaaatcaca actaagtcct caggagcttt atcaagctat tcatcatgaa
    34801 ttagtagcat cggctagagt gacaaaatta gggcgttcta tcaaccctaa ttttaaaatt
    34861 ggctgtatga ttctggctat gccagcttat cctatgacat ctgatccaag agatgttctg
    34921 gctgcaagac aatttgaaca acataatctg ctattttcag atatacatgt cagaggtaaa
    34981 tatcccactt atattcagtc ctatttcaaa aataatggta ttgaaattaa atttgaagaa
    35041 ggagatgagg aagtattggc acaaaataca gtagatttcc tatcgtttag ttactatatg
    35101 agcgtgacac aagcttatga ctttgaaaat tatcaaagtg gtcagggaaa tatccttgga
    35161 ggcctgacta atccttattt aacaacttca gaatgggtat ggcaaatcga tcccataggg
    35221 ctacgcctag tcttaaatca gtattatgaa cgttatcaga ttccgttgtt tattgtagaa
    35281 aatggattgg gagcaaaaga tcaattgata gaaacactag atggtgatta tactgtcgaa
    35341 gatgattatc gtattgatta tatgaatcaa catttagttc aagttgctaa agctattgaa
    35401 gatggtgttg aaataatggg atatacatct tggggatgca ttgattgtgt atccatgtct
    35461 acagctcaac taagtaaacg ttatggtcta atatatgttg atcgaaatga tgatggaaca
    35521 gggagtctac aacgttacaa gaagaagtca tttggttggt accaaaaagt gattaagaca
    35581 aatggccaat cattgtttga acatcataac agataacatg ttaaacaact agtctctgtt
    35641 tcaatttaat ataaaaagga gtatccatag gttactcctt tttattataa tttatgaagg
    35701 taaatatct
//
LOCUS       DATROD010000011        31597 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.011, whole genome shotgun sequence.
ACCESSION   DATROD010000011 DATROD010000000
VERSION     DATROD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31597
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.011"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(23..1804)
                     /locus_tag="VBC74_000540"
     CDS             complement(23..1804)
                     /locus_tag="VBC74_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEO6941513.1"
                     /translation="MLRALKEDFFLTLTSLKNQFWVYIGTGILIQTLVAYLVKGILSF
                     IFRRILILSNTPAVTKDNWTLLFNHPLALLLFILYIVILIGFIYTEFAIYTIAILKTE
                     FSLSIKKWLTIFPKKIKSLLGPQLIWVSIYLLLTIPLANLGLRSSILEHLKIPDFISG
                     ELTKTVFGKIGYSTLLLLICYLNLRLIYFLPLTILTDYNAKEALLESWKLSRGKRQWR
                     LLSKIILTSLFISVIGTVALAVVAGTFSFIDHSGNNFPLQATFYNLLKSILFAVTLLI
                     KCLIISHLLADIKQNKSIVQQWRAWKGVHHKQKFKHIACVILALAIGTTALKNAIALA
                     LLQDNISIKKELIAHRSDTSHGVENSIEALKAAHKAKADYSEMDVIMTKDHKLVVIHD
                     DNLKRLSGMNKDVSKLTLDQVTKIPIHQGHFASYIPSLAEFMKTAQSLDQKIMIELKP
                     YNQNLDIYADEFIKEFKELRLSTKHKVMSLNLTLIEKVEKKLPQLDTGYLIPLHWGTL
                     QNHNVDFYGIEEFSYNDWIAYLAQEYNKQLYVWTINRDNLMIRYLQSPVNGIITDKLN
                     LFKVINKDIKNSPNYYQRALQLIDSEG"
     gene            1974..2576
                     /locus_tag="VBC74_000541"
     CDS             1974..2576
                     /locus_tag="VBC74_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO6941514.1"
                     /translation="MKFLELNKKRHAVKHFNDKPVDFKDVRTAIEIATLAPSANNIQP
                     WKFVVVQEKKSALAEGLPESNCNQINQAQYVIALFTDTDLGQRSRKIARIGRRSLPDD
                     LIGYYMETLPPRYALYSEKQTGEYLSLNAGIVAMNLVLALTDQGISSNMILGFDKAIT
                     NDVLEIDKRFRPEILITVGYSDEKVEPSYRLPVDHIIEKR"
     gene            2623..4029
                     /gene="pepV"
                     /locus_tag="VBC74_000542"
     CDS             2623..4029
                     /gene="pepV"
                     /locus_tag="VBC74_000542"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEO6941515.1"
                     /translation="MTIDFRAEVDKRKDALMDDLINLLRINSERDDSQADAEHPFGPG
                     PVKALEFFLEMAERDGYETKNVDNYAGHFTFGQGEEELGIFGHLDVVPAGSGWDTDPY
                     EPVIKDNRLYARGSSDDKGPTMACYYALKIIKELGLPTSKKVRFVVGTDEESGWGDMD
                     YYFEHVGLPKPDFGFSPDAEFPIINGEKGNITEYLHFSGENKGAVRLHSFSGGLRENM
                     VPESATARFTSHLDQTTLGASLADFAGKHNLKAELSVEDGQYIATVYGKSAHGSIPQE
                     GVNGATYLALYLSQFDFEGPARAFLDVTANIIHEDFSGEKLGVAYEDDCMGSLSMNAG
                     VFQFDETNDDNTIALNFRYPQGTDAKTIQTKLEKLNGVEKVTLSDHEHTPHYVPMDDE
                     LVSTLLAVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPGDENTMHQANEYMPLE
                     NIFRSAAIYAEAIYELIK"
     gene            4126..4710
                     /locus_tag="VBC74_000543"
     CDS             4126..4710
                     /locus_tag="VBC74_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEO6941516.1"
                     /translation="MTDLEKIIKAIKSDSQNQNYTENGIDPLFAAPKTARINIVGQAP
                     GLKTQEAILYWKDKSGDRLRQWLGVDEETFYHSGKFAVLPLDFYYPGKGKSGDLPPRK
                     GFAEKWHPLILKEMPNVQLTLLVGQYAQKYYLGSSAHKNLTETVKSYKDYLPDYLPLV
                     HPSPRNQIWLKKNPWFEKDLIVDLQKIVADILKD"
     gene            4725..5459
                     /locus_tag="VBC74_000544"
     CDS             4725..5459
                     /locus_tag="VBC74_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941517.1"
                     /translation="MRDNHLHTHFSYDCQTAFEDYINGFTGEFITTEHFDLSNPYTGQ
                     DDVPDYSAYCQKIDYLNQKYGNRFKKGIEIGYFKDRESDILDYLKNKEFDLKLLSIHH
                     NGRYDYLQEEALKVPTKELLADYFNRMEFAIGRVEAHVLDHFDYGFRKLNLDVEDLKP
                     FETQLKRIFRKMLSKGLAFELNTKSLYLYGNEKLYRYALEILKQLGCKQYSIGSDGHI
                     PEHFCYEFDRLQCLLKDYQIDENHLI"
     gene            5628..9386
                     /locus_tag="VBC74_000545"
     CDS             5628..9386
                     /locus_tag="VBC74_000545"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEO6941518.1"
                     /translation="MKRKDLFGDKQTQYTIRKLSVGVASVATGVCIFLHSPQVFAEEV
                     SVSPATTAIAKSNINQVDNRQSTNLKDDINSNSETVVTPSDMPDTKQLVSDETDTQKG
                     VTEPDKATSLLEENKGPVSDKNTLDLKVAPSTLQNTPDKTSQAIGAPSPTLKVANQAP
                     QIENGYFRLHLKELPQGHPVESTGLWIWGDVDQPSSNWPNGAIPMTNAKKDDYGYYVD
                     FKLSEKQRKQISFLINNKAGTNLSGDHHIPLLRPEMNQVWIDEKYGIHTYQPLKEGYV
                     RINYLSSSGNYDHLSAWLFKDVATPSTTWPDGSNFVNQGLYGRYIDVPLKTNAKEIGF
                     LILDESKTGDAVKVQPNDYVFRDLANHNQIFVKDKDPKVYNNPYYIDQVQLKDAQQTD
                     LTSIQASFTTLDGVDKTEILKELKVTDKNQNAIQISDITLDTSKSLLIIKGDFNPKQG
                     HFNISYNGNNVTTRQSWEFKDQLYAYSGNLGAVLNQDGSKVEASLWSPSADSVTMIIY
                     DKDNQNRVVATTPLVKNNKGVWQTILDTKLGIKNYTGYYYLYEIKRGKDKVKILDPYA
                     KSLAEWDSNTVNDDIKTAKAAFVNPSQLGPQNLSFAKIANFKGRQDAVIYEAHVRDFT
                     SDQSLDGKLKNQLGTFAAFSEKLDYLQKLGVTHIQLLPVLSYFYVNEMDKSRSTAYTS
                     SDNNYNWGYDPQSYFALSGMYSEKPKDPSARIAELKQLIHDIHKRGMGVILDVVYNHT
                     AKTYLFEDIEPNYYHFMNEDGSPRESFGGGRLGTTHAMSRRVLVDSIKYLTSEFKVDG
                     FRFDMMGDHDAAAIELAYKEAKAINPNMIMIGEGWRTFQGDQGKPVKPADQDWMKSTD
                     TVGVFSDDIRNSLKSGFPNEGTPAFITGGPQSLQGIFKNIKAQPGNFEADSPGDVVQY
                     IAAHDNLTLHDVIAKSINKDPKVAEEDIHRRLRLGNVMILTSQGTAFIHSGQEYGRTK
                     RLLNPDYMTKVSDDKLPNKATLIEAVKEYPYFIHDSYDSSDAINHFDWAAATDNNKHP
                     ISTKTQAYTAGLITLRRSTDAFRKLSKAEIDREVSLITEVGQGDIKEKDLVIAYQTID
                     SKGDIYAVFVNADSKARNVLLGEKYKHLLKGQVIVDADQAGIKPISTPRGVHFEKDSL
                     LIDPLTAIVIKVGKVAPSPKEELQADYPKTQSFKGSKTVEKVNRIANKTSITPVVSNK
                     TDSYLTNEANLPKTGDKSSKILSVVGISILASLLALLGLSLKRNRHLK"
     gene            9521..10438
                     /locus_tag="VBC74_000546"
     CDS             9521..10438
                     /locus_tag="VBC74_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEO6941519.1"
                     /translation="MKNWEKPPLVMAGLVLGLLALGNLLEGYGIYFRYCLGLVALVFW
                     IFLIKGILKNKKESRKELSNPLIASVFTTFFMAGMILSTYILLFRSLGIWVAVLSKGV
                     WWLSFIALIIHMAIFSWKYLRHFSMANLFPSWSVLYVGIGVASLTAPISGQFTIGKIV
                     FWYGFIATLVLLPFLFIKAYKIGLPSAVKPNITTICAPMSLITAGYVNSFVSPNRGLL
                     LLLIVMAQFLYFFILFQVPKLLIGDFTPGFSAFTFPLVISATSLKLSIQHLSLPVDIQ
                     GLVHFEIGTTTLIVMIVMVRYIFFLRRTI"
     gene            complement(10480..10656)
                     /locus_tag="VBC74_000547"
     CDS             complement(10480..10656)
                     /locus_tag="VBC74_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEO6941520.1"
                     /translation="MVKKFAFAKGIATGVVATAATLAGAAFAIKKTIIEPEEEKIAFI
                     EENRKKAARKRVSH"
     gene            10767..11657
                     /gene="miaA"
                     /locus_tag="VBC74_000548"
     CDS             10767..11657
                     /gene="miaA"
                     /locus_tag="VBC74_000548"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEO6941521.1"
                     /translation="MRKIKLIAVVGPTAVGKTALGIELAKTFNGEIISGDSQQVYQKL
                     DIGTAKASKEEQEQAYHHLIDVREVNENYSVYDFVKEAKVAIDTIISKGKIPIIVGGT
                     GLYLQSLFEGYHLGGEVNQETLKAYREKLESLSDEDLFEKLTEQSIVIPQVNRRRAIR
                     ALELAKFGNDLQNSESPYDVLLIGLNDDRQVLYDRINRRVDLMIDNGLLDEAKWLYDN
                     YPSVQASRGIGYKELFPYFSKQIPLEEAVDKLKQNTRRFAKRQLTWFRNRMNVEFIMV
                     GEENYQQKIKRKVSDFLSSK"
     gene            11748..12986
                     /gene="hflX"
                     /locus_tag="VBC74_000549"
     CDS             11748..12986
                     /gene="hflX"
                     /locus_tag="VBC74_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEO6941522.1"
                     /translation="MIETKEEQERVILVGVELQDTENFEMSMEELASLAKTAGANVVN
                     HYYQKRDKYDSKSFIGSGKLEEIKAIVEADEIDTVVVNNRLTPRQNSNLEAELGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQISDIERQLKIVEKNRETVRERRVDSTTFKIGLIGYTNAGKSTIMNVL
                     TDDKQYEANELFATLDATTKQIYLQNQFQVTLTDTVGFIQDLPTELVAAFKSTLEESR
                     HVDLLLHVIDASDPNHEEHEKVVMEILKDLDMIDIPRLAIYNKMDVTEQLNATTFPNV
                     RIAAKKQGSRDLLRRLIVDEIRHIFDEFSIRVHQNQAYKLYDLNKIALLDTYTFEEEY
                     ENITGYISPKQKWKLEEFYD"
     gene            12979..13626
                     /locus_tag="VBC74_000550"
     CDS             12979..13626
                     /locus_tag="VBC74_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEO6941523.1"
                     /translation="MIDYIDLALTYGGFTSLDKVYLEKKLDGLSKQQRLDFITPPPSV
                     INAYFAEIYQKQGPEAATDYYFDLSKALGLFPKHLSFDEEKPFIRLNLSGKSFGFAYL
                     NDQEEASVFSEVKEVITPQLLLEIAQIFPQYKVYQDRSGIRMAKIDFDETESQNITPE
                     TSLLGNVLQLKKDIIKITSFNQEELLELVKTKSGEYYYSSQGRESVIYIDTKSTN"
     gene            13668..14597
                     /locus_tag="VBC74_000551"
     CDS             13668..14597
                     /locus_tag="VBC74_000551"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEO6941524.1"
                     /translation="MEIQFLGTGAGQPAKARNVSSLVLKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKVKKIFITHMHGDHVFGLPGFLSSRAFQANEEQTDLDIYGPVGIKSFV
                     MTGLRTSGSRLPYRIHFHEFDESSLGKIMETDKFTVYAEKLDHTIFCMGYRVVQKDLE
                     GTLDAEALKLAGVPFGPLFGKVKNGENVTLEDGREIIAKDYISEPKKGKVITILGDTR
                     KTDASIRLALGADVLVHESTYGKGDERIAKSHGHSTNMQAADIAKQANAKRLLLNHVS
                     ARFMGRDCWQMEEDAKTIFSNTHLVRDLEEVGI"
     gene            14599..15360
                     /locus_tag="VBC74_000552"
     CDS             14599..15360
                     /locus_tag="VBC74_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEO6941525.1"
                     /translation="MARTILITGASGGLAQAIINQLPQDDHLIVTGRSREKLEKLYGK
                     RPNTLCLSLDITNDNAVTNMIEKIYVEFGQIDILINNAGFGSFKEFWDYSDEEVKDMF
                     AVNTFATMSIARQIGHKMSLVKSGHIVNIASMAGLIATSKASVYGASKFAVVGFLNAL
                     RLELAEKNVYVTSVNPGPIKTGFFAQADPSGDYLASIGRFVLTPEKVAKKVVSILGKN
                     KRELNLPFILAFAHKYYSLFPKTADYFARKVFNYK"
     gene            15357..17555
                     /gene="recJ"
                     /locus_tag="VBC74_000553"
     CDS             15357..17555
                     /gene="recJ"
                     /locus_tag="VBC74_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEO6941526.1"
                     /translation="MISAKYSWVLNNQKPDAGFFEASKKEKISEAVASLIYSRGIKTS
                     AELHHFLQTNLENLHDPYLLNDMDKAVNRIRRAIENNETILVYGDYDADGMTSASIMK
                     EALDMMGAEVQVYLPNRFTDGYGPNQSVYKYFIEQQDVSLIITVDNGVAGHEAIAYAQ
                     NQGVDVVVTDHHSMPADLPCAYAIIHPEHPDANYPFPYLAGCGVAFKVACALLETIPT
                     EMLDLVAIGTIADMVSLTDENRIMVKAGLEVMKDSERIGLQELISLSNIDLKTLNEET
                     IGFKIAPQLNALGRLDDPNPAIELLTGFDDEESQAIAQMIDQKNEERKEIVQTIFDQA
                     MQMLDQTKPVQVLAKENWHPGVLGIVAGRILERTGQPVIVLNIEDGIAKGSARSVEAL
                     DIFQAFDQHRELFIAFGGHSGAAGMTLEESKVGDLSQVLCDYISKKQLDMSQKKTLTI
                     DSELRFDELSLDTVRDFEKLAPFGMDNKKPVFLLKDFKVSQARVMGQNGAHLKLKLEQ
                     DGQALDLVAFNMGSQLQEFQQAQHLELAVTLSVNQWNGATTLQLMLEDARVDGIQLFD
                     IRSKASSLPHGVPILSQEEQSKEVILLTVPDHPQELKQMTQGKQFDAIYFKNEIPKNY
                     FISGYGTRDQFASLYKTIYQFPEFDVRYKLKELSSYLHIPDILLIKMIQIFEELHFVT
                     ITEGIMTVNKEAEKRDISESQIYQELKETVKFQELMALGTPKEIYDFMMS"
     gene            17707..20271
                     /locus_tag="VBC74_000554"
     CDS             17707..20271
                     /locus_tag="VBC74_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GBS Bsp-like repeat-containing protein"
                     /protein_id="HEO6941527.1"
                     /translation="MRPIVRKKMYKKGKFLVIAGIVTILGGSAILGQDVKAEQAEAVT
                     STISEKTDSSQTISDISKLTLPVNSSEAMKNSAEPLIKTGFATSVSSNPREIAATPVK
                     TFDASSKVVVKASTAEHSANQTNSNVNQVANDSEVITQQNSTKQLPTVTYSAHVQDIG
                     WQKSVDNATVSGTVGQEKQVEAIKLSIKAPEGITGKLSYKTYVKGQGWQPSVESGQVS
                     GTVGQSRPIEALSINLTDNLQKLYDVYYRVHVQDIGWMAWAKNGAYAGTLGMSKRLEA
                     YKVKFTLKGQSVLTPTILKEERPVLNYQVKVGQNGWQSNKLEGQMAGTLGESKALDGV
                     KFTLSTLKYGDILYRTHVQDKGWGEILGSQTSKDYQGKRLEAIQLDLSGNLKQNYDIY
                     YRAHVQDKGWMAWQKNNSVAGTVGESKRLEALEVKLVAKASDFSSQTNHSFLEMKHPG
                     LSYQTYLQKDGWKPTVLEGQLGGSIGLSKSIKAIKLNLGSTALGNIEYRTFLNGSGWQ
                     TVVNSGRESNVPNESQQVEAIQMRLTGLLSKTYSVMYRVHMQDYGWQDWTYNNDIAGS
                     TNQGKRIEAIEIKLVETAKMPITVQTTYKGTGNYNVRVTNVISPGNLKIAIWSDKNNQ
                     DDLKWYTVTPKNHEARLTFNVTNHRDNGKYFIHVYQENASNQKQLLVKTSLQVAHSNY
                     NTPYFSQRDGRWASRRYGMATLGETGCVPTSLAMILSSLKGETVLPTQIADYLYHKTV
                     EFNRGVQGTTSRGILKAAKEWGVTATALGTQANLVQALKDGYHVLAAVQNNVFVLHGS
                     HEIVLKGYNNGLTHVSDPYTPSLSGWYPISQLWKEQSYYSEDRIDIGAPFVKVTDA"
     gene            20394..20912
                     /locus_tag="VBC74_000555"
     CDS             20394..20912
                     /locus_tag="VBC74_000555"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEO6941528.1"
                     /translation="MDLNNYIASIENYPQEGITFRDISPLMADGKAYSYAVREIVQYA
                     ADKDIDMIVGPEARGFIVGCPVAYALGIGFAPVRKPGKLPREVISADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVVAGCAFLVELDGLNGRKAIE
                     GYDTKVLMNFPG"
     gene            21030..21710
                     /locus_tag="VBC74_000556"
     CDS             21030..21710
                     /locus_tag="VBC74_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEO6941529.1"
                     /translation="MTYLEQYQSGQLTLPSALFFHFKSIFKTADDFLVWQFFYLQNTT
                     NLSDLTPSRIATSLDKTVADINRSISNLTSQGLLDVKTIELNHEIEIIFDTSPVFAKL
                     DKLFEEDNQVIIDNKTADSNRLKDLVGDFERELGRLLSPFELEDLQKTLQEDQTDPDI
                     VRAALREAVFNGKTSWNYINAILRNWRREGLTTLRQIEERKQAREDNQMKDLAISDDF
                     KNAMNLWE"
     gene            21814..22497
                     /locus_tag="VBC74_000557"
     CDS             21814..22497
                     /locus_tag="VBC74_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEO6941530.1"
                     /translation="MDLQLSKRLQKVANYVPKGARLLDVGSDHAYLPIFLLQMGYCDF
                     AIAGEVVNGPYQSALKNVSEHGLTSKIDVRLANGLSAFEEADNIDTIAICGMGGRLIA
                     DILNNDIDKLQHVKTLVLQPNNREDDLRKWLAANDFEIVAEDILTENDKCYEILVVKH
                     GHMNLTAKELRFGPFLLSNNTTVFKEKWQNELNKLTFALNSIPNSKMEERAILEDKIQ
                     DIKEVLDES"
     gene            22487..23275
                     /locus_tag="VBC74_000558"
     CDS             22487..23275
                     /locus_tag="VBC74_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEO6941531.1"
                     /translation="MKARELIDVYETYCPQELSMEGDISGLQIGSLDKEIKTVMVALD
                     VRETTVAEAIERQVDLLIVKHAPIFRPLKDLVATPQNKIYIDLLKSDIAVYVSHTNID
                     IVPNGLNDWFCELLDIQYPDILSETSNGYGIGRIGDVRPQSFEFFAWKIKDVFGLDSV
                     RLVSYDKSNPEIQRVAICGGSGQSFYKEAIAKGADVFVTGDIYYHTAQEMITNGLLAI
                     DPGHHIEVLFVSKIATMIEQWKLEKGWDISVLESKAPTNPFYHM"
     gene            23284..24387
                     /locus_tag="VBC74_000559"
     CDS             23284..24387
                     /locus_tag="VBC74_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEO6941532.1"
                     /translation="MKKIAIIGAGAVGATLAYYLSKEKDIQVTVFDYGVGQATKAAAG
                     IISPWFSKRRNKAWYRMARLGADFYSKLVTDLQKDGFETKFYQQTGVFLLKKDESQLE
                     SLFALADKRRLESPLIGDLQILNKSEANTHFPELDGYEQLLYASGGARVEGADLTRIL
                     LEASGVNVIKDEVHFTITDNGFRVQGIDFDKLVLASGAWLAKILDEHNYQVDIRPQKG
                     QLRDYYFSNINTGKYPVVMPEGELDIIPFDNGKVSVGASHENDMAFDLNIDFKVLDKF
                     EEQAIGYFPQLKKADTTSERVGIRAYTSDFSPFFGPVPCMEGAYAASGLGSTGLTVGP
                     LIGYELCQLILNKENQLNLEDYDITKYVKKISR"
     gene            24446..25315
                     /gene="rfbA"
                     /locus_tag="VBC74_000560"
     CDS             24446..25315
                     /gene="rfbA"
                     /locus_tag="VBC74_000560"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEO6941533.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSVLMLA
                     GIKEILIISTPQDLPRFEDMLGDGSELGISLSYAEQPSPDGLAQAFIIGEEFIGDDHV
                     ALVLGDNIYHGPGLSAMLQRAASKESGATVFGYQVKDPERFGVVEFDTDMNAISIEEK
                     PAQPKSNYAVTGLYFYDNDVVEIAKNIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRMQNVQVANLEEIAYRMGYITREQVLELAQPLKKN
                     EYGQYLLRLIGEA"
     gene            25315..25908
                     /locus_tag="VBC74_000561"
     CDS             25315..25908
                     /locus_tag="VBC74_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEO6941534.1"
                     /translation="MTEQFFDKELTCRPIEAIPGLLEFDITVRGDNRGWFKENFQKEK
                     MIPLGFPESFFEADKLQNNISFNKKNTLRGLHAEPWDKYVSIADEGRVIGTWVDLREG
                     DSFGNVYQTIIDASKGIFVPRGVANGFQVLSDKAAYTYLVNDYWALELKPKYAFVNYA
                     DPNLGIQWENLEEAEVSEADKNHPLLKDVKPLKKEDL"
     gene            26115..27161
                     /gene="rfbB"
                     /locus_tag="VBC74_000562"
     CDS             26115..27161
                     /gene="rfbB"
                     /locus_tag="VBC74_000562"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEO6941535.1"
                     /translation="MTEFKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGN
                     RANIEAILGDRVELVVGDIADAELVDKLAAKADAIVHYAAESHNDNSLEDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGNGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILAGIKPKLY
                     GEGKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKD
                     AYDHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKD
                     AVEAKYAKTQEVIK"
     gene            complement(27213..30431)
                     /gene="hylB"
                     /locus_tag="VBC74_000563"
     CDS             complement(27213..30431)
                     /gene="hylB"
                     /locus_tag="VBC74_000563"
                     /EC_number="4.2.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_192884539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEO6941536.1"
                     /translation="MEIKKKHRIMLYSALILGTILVNNSYQAKAEELTKTTSTSQIRD
                     TQTNNIEVLQTESTTVKETSTTTTQQDLSNPTASTATATATHSTMKQVVDNQTQNKEL
                     VKNGDFNQTNPVSGSWSHTSAREWSAWIDKGNTADKSPIIQRTEQGQVSLSSDKGFRG
                     AVTQKVNIDPTKKYEVKFDIETSNKAGQAFLRIMEKKDNNTRLWLSEMTSGTTNKHTL
                     TKIYNPKLNVSEVTLELYYEKGTGFVTFDNISMKTKGPKDSEHPQPVTTQIEKSVNTA
                     LNKNYVFNKADYQYTLTNPSLGKIVGGILYPNATGSTTVKISDKSGKIIKEVPLSVTA
                     STEDNFTKLLDKWNDVTIGNYVYDTNDSNMQKLNQKLDETNAKNIEAIKLDSNRTFLW
                     KDLDNLNNSAQLTATYRRLEDLAKQITNPHSTIYKNEKAIRTVKESLAWLHQNFYNVN
                     KDIEGSANWWDFEIGVPRSITGTLSLMNNYFTDAEIKTYTDPIEHFVPDAEYFRKTLV
                     NPFKALGGNLVDMGRVKIIEGLLRKDNTIIEKTSHSLKNLFTTATKAEGFYADGSYID
                     HTNVAYTGAYGNVLIDGLTQLLPIIQETDYKISNQELDMVYKWINQSFLPLIVKGELM
                     DMSRGRSISREAASSHAAAVEVLRGFLRLANMSNEERNLDLKSTIKTIITSNKFYNVF
                     NNLKSYSDIANMNKLLNDSTVATKPLKSNLSTFNSMDRLAYYNAKKDFGFALSLHSKR
                     TLNYEGMNDENTRGWYTGDGMFYIYNSDQSHYSNHFWPTVNPYKMAGTTEKDAKREDT
                     TKEFMSKHSKDAKEKTGQVTGTSDFVGSVKLNDHFALAAMDFTNWDRTLTAQKGWVIL
                     NDKIVFLGSNIKNTNGIGNVSTTIDQRKDDSKTPYTTYVNGKTIDLKQASSQQFTDTK
                     SVFLESKEPGRNIGYIFFKNSTIDIERKEQTGTWNSINRTSKNTSIVSNPFITISQKH
                     DNKGDSYGYMMVPNIDRTSFDKLANSKEVELLENSSKQQVIYDKNSQTWAVIKHDNQE
                     SLINNQFKMNKAGLYLVQKVGNDYQNVYYQPQTMTKTDQLAI"
     gene            30648..31130
                     /locus_tag="VBC74_000564"
     CDS             30648..31130
                     /locus_tag="VBC74_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO6941537.1"
                     /translation="MTKLATICYIDNGKELLLLHRNKKENDVHEGKWISVGGKLEAGE
                     TPDECAKREILEETHLTVKKMDFKGVITFPEFTPGHDWYTYVFKVTDYEGELISDDES
                     REGTLEWVPYDQVLSKPTWQGDYEIFKWILEDVPFFSAKFVYDEHQNLIEKTVNFYEK
                     "
     gene            31120..31581
                     /locus_tag="VBC74_000565"
     CDS             31120..31581
                     /locus_tag="VBC74_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6941538.1"
                     /translation="MKNRDFRVRDQLETFGVRVSALIIENQKLLLIYAPHLYKYYLPG
                     GALQVGEDSNKAVAREVLEEIGLHSQVGDLAYIVENQFNIKRHHYHSVEFLYFVNLLG
                     QAPESIKEGTHKRHFVWLPIKELTKIDCNPNFLAQDLIEWPGHVVHKIVQN"
BASE COUNT        10491 a         4877 c         6257 g         9972 t
ORIGIN      
        1 tctactaagc tttttattta atttaaccct cgctatcaat aagttggaga gctctttgat
       61 aataattagg gctatttttg atatctttat taatgacctt gaaaagattg agtttatctg
      121 tgatgattcc attaactggg ctttgtaaat aacgtatcat aaggttatca cggttaatag
      181 tccaaacata gagctgttta ttatattctt gggcgagata tgcaatccaa tcattgtatg
      241 aaaattcctc aatcccataa aagtcaacgt tatgattttg aagcgttccc caatgtagtg
      301 ggattaggta acctgtatca agttgtggca attttttctc aaccttttca atgagtgtca
      361 agttgagaga cattacttta tgcttggttg atagacgaag ctctttaaat tctttgataa
      421 attcatccgc atagatatcg aggttttgat tatacggttt caattcaatc atgatttttt
      481 gatctagtga ctgagcggtc ttcataaatt ctgccaatga tggaatgtaa ctagcaaaat
      541 gcccttggtg aattgggatt tttgtaactt gatctaaagt tagtttagaa acatctttgt
      601 tcataccaga gagtcgtttg agattatcat catgaatgac tactaattta tgatcttttg
      661 tcatgataac atccatctct gaataatctg cttttgcttt atgggctgct tttaatgctt
      721 caatagaatt ttcaacgcca tgtgatgtat cactacgatg ggctattaat tcttttttaa
      781 ttgaaatgtt atcttgtaag agagccagag ctatagcatt ttttaaggca gtggtaccaa
      841 tagctagagc tagaataaca caagcaatgt gtttaaattt ttgcttatgg tggacgcctt
      901 tccaagctct ccattgttga acaatacttt tattttgttt aatatctgct aataggtgac
      961 ttatgattaa acatttaata agaagtgtta cggcaaaaag tattgatttt aaaaggttat
     1021 agaaagtggc ttgaagagga aaattgtttc cagaatgatc aatgaatgaa aatgtaccag
     1081 caacaacagc tagtgcaact gttccgatta cagatataaa taaactggtt aaaataattt
     1141 tacttagaag acgccactga cgctttccgc ggctaagttt ccaactctct aaaagtgctt
     1201 ctttagcatt gtagtcagtt aaaatggtca gcgggagaaa ataaattaat cgaaggttta
     1261 agtaacaaat aagcagtaaa agggtagagt aacctatctt tccaaagact gtctttgtta
     1321 actcgccaga aataaagtca ggaattttaa gatgctctaa tatacttgac cttagaccaa
     1381 ggttagctag aggaattgtc aaaagtaagt agattgatac ccaaattagc tgtggtccta
     1441 aaagtgattt tattttttta ggaaagattg ttagccattt tttgatactg agagaaaatt
     1501 cagttttcag tatggcaata gtatagatag cgaactcagt atagatgaac ccaattagga
     1561 ttacgatata aagtataaag agtaataaag ctaagggatg attaaaaagc aaagtccaat
     1621 tgtctttagt cactgcggga gtatttgata atatcaatat ccgacgaaag ataaaactca
     1681 gaattccttt tactaaataa gcaactaaag tttggattaa gataccagtt ccaatgtaaa
     1741 cccaaaattg attttttaaa gaggttaagg ttagaaaaaa atcttctttt aatgctcgta
     1801 gcatgaatag ctcctttttt atagtcatta gatattgtac cacaagtttg tttgtttttt
     1861 atggtagttt tcaaaccggt ttcacttttt tatcaatttt gccttcaaaa gctgaaacgc
     1921 attttaaaaa gtgatagaat agtagggaaa atcaaataga ataggaggtt tttatgaaat
     1981 ttctagaatt aaataagaag cgccatgctg tgaaacattt taatgataag ccagtcgact
     2041 ttaaagatgt tagaactgct attgaaattg cgacattagc gccatctgct aataatattc
     2101 agccttggaa gtttgttgtt gtccaagaaa aaaaatctgc tctagcagaa ggtttaccgg
     2161 aaagtaattg taaccaaatt aatcaagcgc agtatgttat tgctttattt acagatacag
     2221 atttaggaca gagatcacgg aaaatagctc gtattggtcg tagatctctt cctgatgatt
     2281 taattggtta ttatatggaa actctaccac cacgctatgc tttatatagt gaaaaacaaa
     2341 ctggagagta tctctcttta aatgcaggta tcgttgctat gaatttagta cttgccttga
     2401 cagatcaagg tattagttct aatatgattt tagggttcga taaagcgatt accaatgatg
     2461 tcttagagat agataaacgt tttagaccag aaatattaat tactgttggt tatagtgatg
     2521 aaaaggttga gcctagttat cgtttgccag ttgatcatat tattgaaaaa cgttaatttt
     2581 taagcacaaa ctacataata ttttatagaa ggagttgccg taatgacaat tgattttaga
     2641 gcagaagtag acaaacgcaa agacgctttg atggacgatt taataaatct tcttcgtatt
     2701 aactcggagc gtgatgattc acaagctgat gcagagcatc catttggtcc tggacctgta
     2761 aaagccttgg aatttttcct tgagatggct gagcgagatg gctacgaaac aaaaaacgta
     2821 gataattatg cgggacactt tacatttggt caaggcgaag aagaactagg tatttttggg
     2881 catttagatg ttgttcctgc aggcagcggt tgggatacag acccttatga accagttatc
     2941 aaggataacc gtttatatgc tcgagggtca tcagacgata agggaccaac aatggcatgt
     3001 tattatgctc taaaaattat taaagagctt gggctaccaa catctaagaa agttcgtttt
     3061 gttgtaggaa ctgatgaaga atctggatgg ggagatatgg attactactt cgaacatgtt
     3121 ggtcttccaa aacctgattt tggtttctct ccagatgcag aatttccaat tataaatggt
     3181 gaaaaaggta atatcactga atatttacat ttttcaggag aaaataaggg agctgttaga
     3241 ctgcatagct tttctggcgg acttcgcgaa aacatggttc cagaatcagc cacagcacgt
     3301 ttcacaagtc atttagatca gacaacactt ggagctagtc tagctgattt tgcaggtaaa
     3361 cataatttga aagcagaatt atctgtagaa gatgggcagt atatagctac agtttatggt
     3421 aaatctgctc atggttcaat accacaagaa ggtgttaatg gggcgactta tttagctctt
     3481 tatctaagtc aatttgattt tgaaggtcct gctcgtgctt tcttagatgt cacagccaac
     3541 attattcacg aagacttctc aggtgaaaaa cttggagtag cttatgaaga tgactgtatg
     3601 ggatcgttga gcatgaatgc aggtgtcttc cagtttgatg aaactaatga tgataatact
     3661 atcgctctta atttccgtta cccacaaggg acagatgcta aaactatcca aactaagctt
     3721 gagaaactta acggagttga aaaagtgact ctttctgacc atgaacacac accacactat
     3781 gtacctatgg acgatgaatt agtatcaacc ttactagctg tctatgaaaa gcaaactggt
     3841 cttaaaggac atgaacaggt tattggtggg gggacatttg gtcgcttact tgaacggggt
     3901 gttgcatacg gtgccatgtt cccaggagat gaaaacacta tgcatcaagc taatgagtac
     3961 atgcctttag aaaatatttt ccgttcggct gctatctacg cagaagctat ctatgaatta
     4021 atcaaataaa ataatcctta aactaaatat gtgatcaatg ataaagggtg gtgaagacat
     4081 gaaagtgtct ttgcctcttt tcataaggtt agatttggag actttatgac tgacttggaa
     4141 aaaattatta aagcaataaa aagtgattca cagaatcaaa attatacaga aaatggtatt
     4201 gatcctttgt ttgctgctcc taaaacagct aggatcaata ttgttggaca agcacctggt
     4261 ttaaaaactc aagaagcaat actctattgg aaagataaat ctggagatcg tctacgccag
     4321 tggcttggag ttgatgaaga gacattttac cattctggaa aatttgctgt tttgccttta
     4381 gatttttatt acccaggcaa aggaaaatca ggagatttac cccctagaaa aggttttgcg
     4441 gagaaatggc accctcttat tttaaaagaa atgcctaatg ttcaattgac cttgctagtt
     4501 ggtcaatatg ctcagaaata ttatcttgga agctccgcac ataaaaatct aacagaaaca
     4561 gttaaatctt acaaagacta tctacccgat tatttacccc tggttcaccc atcaccgcga
     4621 aatcaaattt ggctaaagaa gaatccatgg tttgaaaaag atctaatcgt tgatttacaa
     4681 aagatagtag cagatatttt aaaagattaa ggataggagt tagtatgaga gataatcatc
     4741 tacacacgca tttttcctat gattgtcaaa cggcatttga ggactatatt aatggtttta
     4801 caggtgaatt tatcacaaca gaacattttg atttatcaaa tccttacacc ggtcaagacg
     4861 atgttcctga ttatagtgct tattgtcaaa aaatagatta tcttaatcag aaatatggaa
     4921 atcgatttaa aaaaggaatt gaaatcggtt attttaaaga tagggaatca gatattttag
     4981 attatttaaa aaataaagaa tttgatttaa aactattgtc aatccatcat aatggtaggt
     5041 atgattatct gcaagaagaa gctctgaaag taccaacaaa ggagctttta gcagattact
     5101 ttaatcgtat ggaatttgcc ataggccgtg tggaagcgca cgttttagat cactttgatt
     5161 atggttttcg taagttaaac ttagatgtag aagatttaaa accgtttgaa acgcaattga
     5221 agcgcatttt cagaaagatg ttatctaagg ggttagcttt tgaactaaat accaaatccc
     5281 tttatctata tgggaatgaa aaactttatc gctatgcttt agagatacta aaacagcttg
     5341 gttgtaaaca atactctata ggctctgacg gtcatattcc tgaacatttt tgttatgaat
     5401 ttgatagact tcaatgtctg ctaaaggact atcaaattga tgaaaatcat ttgatatgag
     5461 gaaatttttg ataaaaaagc taggcaatat tgcttagctt ttttgtaatg tcattgatag
     5521 ttttagtgaa aatttcaaaa aaataaagaa atcacttact tgttgcaagc gcttgcgtaa
     5581 attgttatga ttttattggt aacaattcat taaaaaagga gaatgatatg aaaagaaaag
     5641 acttatttgg tgataaacaa actcaataca cgattagaaa gttaagtgtt ggagtagctt
     5701 cagttgcaac aggggtatgt atttttcttc atagtccaca ggtatttgct gaagaagtaa
     5761 gtgtttctcc tgcaactaca gcgattgcaa agtcgaatat taatcaggtt gacaaccgac
     5821 aatctactaa tttaaaagat gacataaact caaactctga gacggttgtg acaccctcag
     5881 atatgccgga taccaagcaa ttagtatcag atgaaactga cactcaaaaa ggagtgacag
     5941 agccggataa ggcgacaagc ctgcttgaag aaaataaagg tcctgtttca gataaaaata
     6001 ccttagattt aaaagtggca ccatctacat tgcaaaatac tcccgacaaa acttctcaag
     6061 ctataggtgc tccaagtccg accttgaaag ttgctaatca agctccacag attgaaaatg
     6121 gttactttag gttacatctt aaagaattgc ctcaaggtca tcctgtagaa agcactgggc
     6181 tttggatatg gggagatgtt gatcaaccgt ctagtaattg gccaaatggt gctatcccta
     6241 tgactaatgc taagaaagat gattacggtt attatgttga ttttaaatta tctgaaaaac
     6301 aacgaaaaca aatatctttt ttaattaata acaaagcagg aacaaattta agcggcgatc
     6361 atcatattcc attattacga cctgagatga accaagtttg gattgatgaa aagtacggta
     6421 tacatactta tcagcccctc aaagaagggt atgtccgtat taactatttg agttcatctg
     6481 gtaactatga ccacttatca gcatggctct ttaaagatgt tgcaaccccc tcaacaactt
     6541 ggccagatgg tagtaatttt gtgaatcaag gactatatgg aaggtatatt gatgtaccac
     6601 tgaaaactaa tgccaaagag attggttttc taatcttaga tgaaagtaag acaggagatg
     6661 cagtgaaagt tcaacccaac gactatgttt ttagagattt agctaaccat aaccaaattt
     6721 ttgtaaaaga taaggatcca aaggtttata ataatcctta ttacattgat caagtgcagc
     6781 taaaggatgc tcaacaaact gatttaacaa gtattcaagc aagttttaca actctagatg
     6841 gggtagataa aactgaaatt ttaaaagaat tgaaagtgac agataaaaat caaaatgcta
     6901 tacaaatttc tgatatcact ctcgatacta gtaaatctct tttaataatc aaaggcgact
     6961 ttaatcctaa acaaggtcat ttcaatatat cttataatgg taacaatgtc acgacaaggc
     7021 aatcttggga atttaaagac caactttatg cttatagtgg aaatttaggt gcagttctca
     7081 atcaagatgg ttcaaaagtt gaagccagcc tctggtcacc gagtgctgat agtgtcacta
     7141 tgattattta tgacaaagat aatcaaaaca gggttgtagc gactaccccc cttgtgaaaa
     7201 ataataaagg tgtttggcag acgatacttg atactaaatt aggtattaaa aactatactg
     7261 gttactatta tctttacgaa ataaaaagag gtaaggataa ggttaagatt ttagatcctt
     7321 atgcaaagtc attagcagag tgggatagta atactgttaa tgacgatata aaaacggcta
     7381 aagcagcttt tgtaaatcca agtcaacttg gacctcaaaa tttaagtttt gctaaaattg
     7441 ctaattttaa aggaagacaa gatgctgtta tatacgaagc acatgtaaga gacttcactt
     7501 ctgatcaatc tttggacgga aaattaaaaa atcaacttgg tacctttgca gccttttcag
     7561 agaaactaga ttatttacag aaattaggag ttacacacat tcagctttta ccggtattga
     7621 gttattttta tgttaatgaa atggataagt cacgctcaac agcttacact tcctcagaca
     7681 ataattacaa ttggggctat gacccacaga gctattttgc tctttctgga atgtattcag
     7741 agaaaccaaa agatccatca gcacgtatcg ccgaattaaa acaattaata catgatattc
     7801 ataaacgtgg catgggggtt atacttgatg tcgtctataa tcacactgca aaaacttatc
     7861 tctttgagga tatagaacct aattattatc actttatgaa tgaagatggt tcaccaagag
     7921 aaagttttgg agggggacgt ttaggaacca ctcatgcaat gagtcgtcgt gttttggttg
     7981 attccattaa atatcttaca agtgaattta aagttgatgg tttccgtttt gatatgatgg
     8041 gagatcatga tgcggctgcg attgaattag cttataaaga agctaaagct attaatccta
     8101 atatgattat gattggtgag ggctggagaa cattccaagg cgatcaaggt aagccggtta
     8161 aaccagctga ccaagattgg atgaagtcaa ccgatacagt tggcgtcttt tcagatgata
     8221 ttcgtaatag cttgaaatct ggttttccaa atgaaggtac tccagctttc atcacaggtg
     8281 gcccacaatc tttacaaggt atttttaaaa atatcaaagc acaacctggg aattttgaag
     8341 cagattcgcc aggagatgtg gtgcagtata ttgctgcaca tgataacctt accttgcatg
     8401 atgtgattgc aaaatcaatt aataaagacc ctaaggtagc tgaagaagat attcatagac
     8461 gtctgcgttt aggaaatgta atgattttaa catctcaagg gacagcattc attcattctg
     8521 gtcaagagta tggtcgtacg aagcgtttac ttaaccctga ttacatgaca aaagtttcag
     8581 atgacaaatt gcctaataaa gcaacactta ttgaagctgt taaagaatac ccatatttta
     8641 ttcatgattc atatgattct tcagatgcca ttaatcattt tgattgggca gcagccacag
     8701 ataataacaa acacccaatt tcaacgaaaa cacaggccta tacagcaggt ttaatcacat
     8761 taaggcgttc aacagatgct ttccggaaat tgagcaaagc agaaattgat cgtgaggtta
     8821 gcttgattac agaggtaggt caaggtgata ttaaagaaaa agatttggtt attgcttacc
     8881 aaacaataga ttctaaaggc gatatttacg cagtatttgt taatgctgat agtaaagcta
     8941 gaaacgtttt actaggtgaa aaatataaac accttttaaa agggcaagta attgttgatg
     9001 ctgatcaagc ggggattaaa ccaatctcaa ctcctagagg tgttcatttt gaaaaagata
     9061 gtttgctgat tgatccatta acagcaattg tgattaaagt tggcaaagtt gctcctagcc
     9121 ctaaggagga attgcaagca gattatccca aaacacaatc tttcaaggga tctaaaacgg
     9181 tagaaaaagt aaatagaata gctaataaga cctcaataac tcctgtagtt tctaataaga
     9241 ccgattcata tctgacaaat gaagctaatt tgccaaaaac tggagataag tcatcaaaaa
     9301 tactaagtgt agtaggaata agcattctag caagtctact tgctctacta ggtctctctt
     9361 taaagaggaa tcgtcactta aaataaaagc aaactttaga agagcttaaa aaagaagtta
     9421 gtcaatgggc tagcttcttt tcattaagtt aataaaatca cttatcagca aaatctaatt
     9481 gtgttataat ggatgtaaac gtttttttat gaggtatatg atgaaaaatt gggaaaaacc
     9541 accattagtg atggcgggat tagtattggg gctattagct ttggggaacc ttctagaggg
     9601 atatggcatc tattttcgct attgtttagg gctagtagca cttgtttttt ggattttttt
     9661 aatcaaagga atattaaaaa ataaaaaaga aagcaggaag gagttaagca atcctttgat
     9721 tgcttctgtc tttactactt tttttatggc aggtatgatt ttatcgacct atatcttatt
     9781 atttagaagt ttaggaatat gggtagcagt tctgtcaaaa ggtgtatggt ggttgtcatt
     9841 tattgcgtta atcattcata tggctatttt ttcatggaaa tatttgagac acttttcgat
     9901 ggctaatctt tttccttctt ggtctgtcct ctatgttggt ataggggtag ctagcctgac
     9961 agcaccaatt agtggacaat tcactattgg gaaaatagtt ttttggtatg gttttattgc
    10021 aactttagta ttattaccat ttctattcat taaagcatat aaaatcgggc taccttcggc
    10081 agttaagcct aatataacta ctatttgcgc tccaatgtcg ctaatcacag cgggatatgt
    10141 taatagtttt gtgtcaccaa atagaggttt attattgcta ttaatcgtaa tggcacaatt
    10201 tctttatttt ttcattcttt ttcaggtgcc taaattactg attggtgatt ttacaccagg
    10261 tttctcagcc tttactttcc cccttgttat ctcagctacc tcacttaaat taagtattca
    10321 gcatctatca ttaccagttg atatacaggg gctagtccac tttgaaattg ggacaacaac
    10381 tcttattgtt atgatagtaa tggtacgtta tattttcttt ttaaggcgaa caatttaata
    10441 taaaataact cccttactag ctagtaaggg agttattgtt taatgagaga cacgtttacg
    10501 tgcagctttt ttacgatttt cttcgataaa agctattttt tcttcctcag gttcaataat
    10561 tgtttttttg atagcaaatg cagcacctgc aagagtagca gctgttgcaa cgactcctgt
    10621 tgctattcct ttagcaaatg cgaatttttt aaccataata attctccaat ctatgatgaa
    10681 gactctgctt ctatctatga tataattatg gtaacgaaat actagttaaa tttcaagtaa
    10741 aaacctttat agaattgaga agtatcatga gaaaaatcaa gcttatagca gttgttggtc
    10801 caactgcggt tgggaagaca gcattaggaa ttgaattagc taaaactttc aatggcgaaa
    10861 tcattagtgg cgatagtcag caagtttatc agaagttaga tatcggaacg gctaaagcta
    10921 gtaaagaaga gcaagaacaa gcttatcatc acttgattga tgttcgtgaa gtaaatgaga
    10981 attattcggt ttatgatttt gtcaaagagg caaaagtagc tattgatacg attatttcta
    11041 aggggaaaat ccctattatt gttggaggta ctggcctata tttacagagt ttgtttgagg
    11101 gctatcattt aggaggcgaa gtcaatcaag aaactcttaa ggcctatcgt gaaaaattag
    11161 agtctctaag tgatgaagat ttatttgaaa agttgacaga acaatccatt gttataccgc
    11221 aagttaatcg tcgacgtgcc atccgtgcat tagaattggc gaagtttggg aatgatttgc
    11281 aaaatagtga aagtccgtat gatgttttat taattggatt gaatgatgat cgtcaggtgc
    11341 tctatgatcg tatcaataga cgagttgatt tgatgataga caatggtctc ttagatgaag
    11401 ctaaatggct atacgataac taccctagtg ttcaagcaag tagaggaatt ggctataaag
    11461 aattatttcc ttacttttca aagcaaatac cattagaaga agctgttgat aaactaaaac
    11521 aaaataccag acgttttgca aaacgccaat taacatggtt tagaaaccgt atgaatgtcg
    11581 aatttattat ggttggtgag gaaaattatc aacaaaaaat caagcgaaaa gtcagtgatt
    11641 ttttatcatc aaagtaaata aagccgtttc tcgcatttaa agggcattat gttataataa
    11701 agattacgta tttagaacaa gaaaaaaata ttgaaaggag attataggtg atagaaacta
    11761 aagaagagca agaacgtgtt attttagttg gggtagaact tcaagatact gagaattttg
    11821 aaatgtccat ggaagaactt gctagcttag ctaagacagc aggagcaaat gttgttaatc
    11881 attattatca aaaaagagat aaatatgata gtaaatcatt tatcggatct ggtaaattag
    11941 aggagataaa agctattgta gaagcagatg agattgatac cgttgtggtt aataatcgtt
    12001 tgactccgcg acaaaactct aatttggaag ctgaacttgg tgttaaagtt attgaccgta
    12061 tgcaacttat tttagatatc tttgctatgc gtgctagaag tcatgaagga aaattacagg
    12121 ttcatctagc gcaattgaaa tatatgttac cacgcttagt tggacaaggt attatgctta
    12181 gtcgtcaagc aggaggaatc ggaagccgag gtccaggaga atctcagtta gagttgaatc
    12241 gtcgttccat tcgtcatcaa atttctgata tcgaaagaca attgaagatt gtagaaaaaa
    12301 atcgcgagac agttcgagaa agacgtgttg attcgacaac ctttaaaatt ggattaattg
    12361 gatatacaaa tgctggcaag tcaactatta tgaatgtttt gaccgatgat aagcaatatg
    12421 aagccaatga attatttgca acgttagacg ctacaaccaa gcagatctat ttacaaaatc
    12481 aatttcaagt cactttaaca gatactgttg gttttattca agatttacct acagagttag
    12541 ttgcggcttt caagtctacc ttagaagaaa gtcgacatgt tgatttatta cttcatgtta
    12601 ttgatgctag tgaccctaat catgaagaac acgaaaaagt tgttatggag attcttaaag
    12661 acttagatat gatagatatt cctcgtttag cgatatacaa caagatggat gtcactgagc
    12721 agctgaatgc aacaactttt cctaatgtga gaattgcagc caaaaaacaa gggagtaggg
    12781 atttattacg ccgtcttatt gtggatgaaa tacgacatat ctttgacgaa ttttcgatta
    12841 gagtgcatca aaatcaagca tataagctct atgatctcaa taaaatagca ttgcttgaca
    12901 cctacacctt tgaagaagaa tacgagaata ttacaggcta tatttctcct aaacaaaaat
    12961 ggaagctgga agaattttat gattgattat attgatttgg ctttgactta tggtggtttt
    13021 acgagcttag ataaagttta tctagagaaa aaactagatg gcctctctaa gcaacaacga
    13081 cttgatttta tcactccacc tcctagtgtg attaatgctt attttgctga aatatatcaa
    13141 aagcaagggc cagaagcggc aactgattac tattttgatc tttccaaagc cttgggcttg
    13201 tttccaaaac atttatcgtt tgatgaagaa aaacctttta ttcgtttgaa tttatcagga
    13261 aaatcatttg gttttgctta cctaaatgat caagaagaag cgagtgtttt ttctgaagtt
    13321 aaagaagtca ttacaccgca gttactctta gaaattgcac agatttttcc tcaatataaa
    13381 gtctatcaag atcggtctgg tatcagaatg gcaaaaatag attttgacga aacagaatct
    13441 caaaatatta caccagaaac aagtttacta gggaatgtct tacaacttaa aaaagatatt
    13501 attaaaataa ctagtttcaa tcaagaagag ctattagagt tggttaaaac aaagtcaggt
    13561 gaatactatt attctagtca aggacgtgag tcagttattt atattgatac taaatctact
    13621 aattaaattt attaagttat ggaaggatta taaaaaagtc aacaagtatg gaaattcaat
    13681 ttttaggaac tggagctggt caaccagcga aagctcgtaa tgtttcaagt ttagtcttga
    13741 aattattaga tgaaattaac gaggtttgga tgtttgattg tggagagggt acacagcgtc
    13801 aaattttaga aaccacgatt aaacctcgta aagtcaaaaa aatttttatc acgcacatgc
    13861 acggagacca tgtttttggt cttccaggtt ttttatccag tcgggctttt caagccaatg
    13921 aagaacagac agatttagat atttatggac cggtcggtat aaaaagtttt gttatgactg
    13981 gcttaaggac atcaggttca cgtttacctt atcgaattca ttttcacgag tttgatgaat
    14041 catcactcgg aaaaattatg gaaacagata aatttaccgt ttatgcagaa aagctagatc
    14101 atactatctt ttgtatggga tatcgtgttg tgcaaaaaga ccttgagggc actttagatg
    14161 ctgaagcatt gaaactagca ggtgttccat ttggtcccct ttttgggaaa gtcaaaaatg
    14221 gtgagaatgt taccttagaa gatggtagag aaattattgc taaagattat atttctgaac
    14281 cgaaaaaggg taaagtaatc actattttgg gagatactag aaaaacagat gcaagtatta
    14341 ggttagcttt gggcgctgac gttttagtgc acgaatctac ttatggtaag ggtgatgagc
    14401 gaattgctaa atcgcatggg cattcaacca atatgcaagc agctgatatc gctaaacaag
    14461 caaatgctaa acgactatta ttaaatcatg ttagtgctcg ttttatgggg cgagattgtt
    14521 ggcaaatgga agaagatgca aagacgattt tttcaaatac ccatcttgtt agagatttgg
    14581 aagaagtagg tatataaaat ggcaagaacg atactgatta caggagcttc tggtggttta
    14641 gcacaggcaa ttattaacca attacctcaa gacgaccatt taattgtcac agggagatca
    14701 agagaaaaac ttgaaaagct ttatggaaag agaccaaaca ccttatgttt atcactagac
    14761 ataactaatg acaatgctgt aacgaatatg attgaaaaaa tttatgttga atttggtcaa
    14821 attgatattt taattaataa tgctggattt ggttctttta aagaattttg ggattatagt
    14881 gatgaagaag tcaaagacat gtttgctgtg aatacatttg caacgatgtc tatagctcgt
    14941 cagataggac ataagatgtc tcttgtaaaa tctggtcata tcgttaatat tgcaagcatg
    15001 gctggcctca tagcaacaag taaagctagc gtttatgggg ctagtaagtt tgctgtcgtt
    15061 ggatttttga atgctcttcg tttagaatta gcagaaaaaa atgtttatgt gacaagtgta
    15121 aaccctggac caatcaaaac aggatttttt gctcaggctg atccaagtgg tgattattta
    15181 gcaagtatag gtcgttttgt tctaacacca gaaaaagtag ctaaaaaagt agtttctatt
    15241 cttggaaaaa ataagagaga attaaatctt ccttttatat tagcttttgc gcataaatat
    15301 tatagtttat ttccaaaaac agctgattat tttgccagaa aggtattcaa ttataaatga
    15361 tatctgctaa gtatagttgg gtcctcaata accaaaagcc agacgctggc ttttttgagg
    15421 cttcaaaaaa agaaaagata agtgaagcgg tggcttcctt aatttattcg cgaggtatta
    15481 agacatcagc ggaattacat cattttttac aaactaattt ggaaaatctc catgatcctt
    15541 atttgttaaa tgatatggat aaagctgtta atcgtattag acgagctatt gaaaataatg
    15601 aaactatttt agtttatgga gactatgatg ctgatggaat gacatcagca tcaatcatga
    15661 aagaagcatt agatatgatg ggagctgaag tccaggttta tctcccaaat cgctttacag
    15721 atggttacgg tcccaatcaa agtgtttata aatattttat tgaacaacaa gatgtttcat
    15781 taatcattac ggttgataat ggtgtggcag ggcatgaagc aattgcatat gctcaaaatc
    15841 aaggtgtgga cgtggtagtt actgatcatc acagtatgcc agcagattta ccttgtgctt
    15901 atgctattat tcacccagaa catcctgatg ctaattatcc atttccttat ttagcagggt
    15961 gtggtgttgc tttcaaagta gcttgtgctc tcttagaaac aatcccgaca gaaatgttgg
    16021 atttggttgc tattgggacg attgcagata tggttagcct aactgatgaa aatcgtatca
    16081 tggtaaaagc tggacttgaa gttatgaaag atagtgaaag aataggctta caggagctaa
    16141 tatctttatc aaatattgat ttaaaaacac taaatgaaga aactattgga tttaaaattg
    16201 ctccacaatt aaacgcttta ggaagacttg atgaccctaa tccagctatt gaattactaa
    16261 cagggtttga tgatgaagaa tctcaagcaa tagcccaaat gattgatcaa aaaaatgaag
    16321 agcgcaaaga aatcgttcaa actatctttg accaagctat gcaaatgctt gatcaaacaa
    16381 agcctgtaca agttttagca aaagaaaatt ggcatccagg ggttttaggt attgtggcgg
    16441 gtcgtatttt agagagaact ggacaacctg ttattgtttt gaatattgaa gatggtattg
    16501 ctaaaggaag tgccagaagt gtagaagcac ttgatatttt tcaagctttt gaccagcatc
    16561 gagaattatt cattgctttt ggaggtcaca gcggagcagc aggcatgact ttagaggaaa
    16621 gtaaggtagg tgatctttct caagttttat gtgactatat ctctaaaaaa caattggaca
    16681 tgagtcagaa aaaaactctg acaattgata gtgagctacg ttttgacgaa cttagtttag
    16741 acactgttag agattttgaa aagttagcgc catttggaat ggataataaa aaaccagtct
    16801 ttttgttaaa agattttaaa gtgagtcaag ccagagtcat ggggcaaaac ggtgctcatc
    16861 tgaaattaaa actagaacaa gatggacaag cacttgattt ggtagcattt aatatgggga
    16921 gtcaactaca agagtttcaa caagcacaac atttggagtt agctgtcact ttatctgtta
    16981 atcaatggaa tggtgccaca accctacaat taatgttgga ggatgcgcgt gtagatggta
    17041 ttcaattatt tgatattaga agcaaagcaa gtagcttacc tcatggagtg ccaatcctgt
    17101 ctcaagaaga gcagagcaaa gaagttattc ttttgactgt tcccgatcat ccacaagaat
    17161 taaaacaaat gactcaaggt aaacagtttg atgctatcta ttttaaaaat gagataccaa
    17221 aaaattattt tatatctggt tatggtacac gtgatcaatt tgctagttta tataaaacaa
    17281 tatatcaatt cccagaattt gacgttcgtt ataagttaaa agagctcagt tcctaccttc
    17341 atattcctga tattttatta attaaaatga ttcaaatttt tgaagaacta cattttgtta
    17401 cgattacaga ggggattatg acagtcaata aagaagcaga aaagcgagat atctctgaga
    17461 gtcaaattta tcaagaatta aaagagacag ttaagtttca agaattaatg gctttaggga
    17521 cacctaaaga aatatatgat tttatgatga gttgataata tactaaagaa gccacttttt
    17581 aagtggtttt tttggtatat aaataagtta tttcaataat aatattgcaa atattattaa
    17641 aatataataa aattaagaaa ataatggaat ttttgataaa gaatattaaa ggtgaggata
    17701 aaatctatga gacctatcgt tagaaaaaag atgtacaaaa aaggtaaatt tttggtcatt
    17761 gcaggtatcg ttactatttt aggtggtagt gcaattcttg gtcaagatgt caaggcagaa
    17821 caggcagaag cagtaacttc aacgatctca gagaaaactg atagcagtca gactatttcg
    17881 gatatttcca agttaacgtt acctgttaat tcctcggagg ctatgaaaaa tagtgcagag
    17941 cctttaataa aaacaggctt tgctacatct gtttcatcta atcctagaga gatagcagct
    18001 accccagtca aaacctttga tgctagttca aaagttgttg taaaagctag tactgctgag
    18061 cactcagcaa atcaaacaaa ttctaatgtt aatcaagtag ctaatgatag tgaagttatt
    18121 actcaacaaa attcaacaaa gcaattacca acagtgactt actctgctca tgttcaagat
    18181 attggatggc aaaagtcggt tgataatgca acagtttcag gaacagtagg acaggaaaag
    18241 caggttgagg caataaaatt gtctattaaa gcccctgaag gaataacagg aaaacttagc
    18301 tataaaactt atgttaaagg acaaggttgg caaccttcag tagagagtgg tcaagttagt
    18361 ggtactgttg gtcagagtag acctattgaa gcactaagca ttaaccttac tgataattta
    18421 caaaaattat atgatgtcta ttatcgtgtt catgttcaag atatcgggtg gatggcttgg
    18481 gctaaaaatg gagcttacgc tgggactttg ggaatgtcaa agcgccttga agcttacaaa
    18541 gtaaagttta cccttaaagg acaatctgtt ttaacaccaa ctatacttaa ggaagagaga
    18601 cctgtcttaa attatcaggt taaggtaggc caaaatggtt ggcaatctaa taaacttgaa
    18661 ggacaaatgg cgggaacttt aggagaatcc aaagccctag atggagtaaa attcactctt
    18721 agcaccttga agtatgggga cattctctat cgtactcatg tacaagataa aggttgggga
    18781 gaaattttag gtagtcaaac aagtaaggat tatcaaggaa aaagactaga agctattcaa
    18841 ttggacttaa gtgggaattt aaaacaaaat tatgatattt attatcgcgc tcacgtccaa
    18901 gataaaggtt ggatggcttg gcaaaaaaat aatagtgtag caggtacagt cggagaatcg
    18961 aaacgtttag aggctttgga agtaaaacta gtagctaaag cttcagattt tagttcccaa
    19021 acaaatcatt cattcttaga aatgaagcat cctggattaa gttatcaaac ttatcttcaa
    19081 aaagatggtt ggaaaccaac ggttttggaa ggccaattag gaggtagtat tggactttca
    19141 aaatctataa aagccatcaa acttaactta ggtagtacag ctctaggaaa tatagaatat
    19201 agaacttttc ttaatggttc aggttggcaa actgttgtta attcagggag agaaagtaat
    19261 gtaccaaatg aatctcaaca agtagaagct attcagatgc gattgacagg tctgctatcg
    19321 aaaacctata gtgtgatgta tcgagttcac atgcaagatt atggttggca ggattggact
    19381 tataacaatg acatcgctgg tagtactaac caaggaaaac gaattgaagc aatcgaaatt
    19441 aaattagttg agactgctaa aatgcctata actgtccaaa cgacctataa gggaactgga
    19501 aactataatg tacgagtgac aaatgttatt agtcctggga atttaaaaat tgctatttgg
    19561 tcagataaaa acaatcagga tgaccttaag tggtataccg tcacacccaa aaatcatgaa
    19621 gctagattga catttaatgt tacaaatcat agagacaatg gtaaatactt tatccatgtt
    19681 tatcaggaaa atgcttctaa tcaaaaacaa ttattggtaa aaacttctct acaagtcgca
    19741 cattcaaatt ataatactcc ttactttagc caacgtgatg gaaggtgggc aagtcgaaga
    19801 tatggtatgg caactttagg agaaactggt tgtgtaccaa cttctctagc tatgattttg
    19861 tcttctttga aaggagaaac tgttttacca acgcaaattg cagattacct ttaccataag
    19921 acagttgaat ttaatcgtgg tgttcaagga acaacttcta gaggaatctt aaaagcagcc
    19981 aaagaatggg gtgtaacagc aactgcatta ggaactcaag ctaaccttgt acaagcgttg
    20041 aaggatggtt atcatgtctt agcggcagta caaaataatg tttttgttct tcatggaagt
    20101 catgaaatcg ttctcaaagg atataataac ggactgacac atgtctctga cccttacaca
    20161 ccgtccttgt ctggttggta tccaatttca caactttgga aagaacagag ctattatagt
    20221 gaggatagaa ttgatattgg agcacctttt gtcaaggtaa cagatgccta atttttaaaa
    20281 ccatatgcaa aactagcata tggttttaaa gtttccagta ctattttatt taattttatg
    20341 atataatgaa gagtaaaaca ttttaggggt ttccccttag aaagacaagt gaaatggatt
    20401 taaataatta catcgcttca attgaaaact atcctcaaga aggaattact ttccgtgata
    20461 tctccccatt aatggctgat ggtaaagcat acagctatgc tgttcgtgaa attgtacaat
    20521 atgctgctga taaagatatt gatatgattg ttggaccaga agctcgtggc tttattgttg
    20581 gttgcccagt tgcttatgct cttggtattg gttttgctcc agtccgtaaa ccaggtaaat
    20641 taccacgcga agttatttca gctgactatg aaaaagaata tggtcttgat acattgacaa
    20701 tgcacgcaga tgctattaaa ccaggtcaac gtgttttaat tgttgatgac cttttagcta
    20761 ctggtggaac agtaaaagcg acaattgaaa tgattgaaaa acttggtggc gttgtcgcag
    20821 gctgtgcttt cctagttgaa cttgatggat taaatggtcg taaagctatt gaaggttatg
    20881 atactaaggt attgatgaac tttccaggat aaggcctatt caagatagat gaatgataag
    20941 ggcgcctcta agtgctatga taatagttga gaggtgcttt ttgttacaca tttcataatt
    21001 ttaggacgat agagaggatt tgaaaggtta tgacttattt ggaacaatat cagtcagggc
    21061 aattgactct accaagtgct ttattttttc actttaaatc tatttttaag acagcagacg
    21121 attttttagt ttggcaattt ttctatttac aaaatacaac taatttatcc gatctaacac
    21181 cgagtcgtat tgcgacatca cttgataaaa cagtggcaga tatcaatcgt tctatatcga
    21241 atttaacaag tcaaggttta ctagatgtca aaacaattga gttgaatcac gaaatcgaga
    21301 ttatctttga tacaagccct gtgtttgcta aattagataa acttttcgaa gaggataatc
    21361 aagtaataat agacaataaa accgctgatt cgaatcgtct gaaagattta gtaggagact
    21421 tcgaacgtga attaggtcgc ttattaagtc cttttgaatt agaagattta caaaagacac
    21481 tgcaagaaga tcagacagat cctgatattg ttagggcagc tcttagagaa gcggttttca
    21541 atgggaaaac aagttggaat tatatcaatg ctatccttcg caactggcgg cgtgaaggat
    21601 tgacgacact tcgacaaatt gaagagagaa aacaagcacg tgaagataac caaatgaaag
    21661 atttagcgat ttcggatgat tttaaaaatg caatgaattt atgggaataa gtttactagg
    21721 aggagacaga atggttgata cattaggtct gctccttttc ttttgatgtt aatgatgtta
    21781 tacttttaac agagaaaaac tgaaggaaat acaatggatt tacaattatc aaaacgatta
    21841 caaaaagttg caaactatgt tccaaaagga gctcggttat tagatgtagg aagtgatcat
    21901 gcttacttac ctattttttt gttgcagatg ggatactgtg attttgccat tgctggtgaa
    21961 gtggtgaatg gaccgtatca gtctgcgttg aaaaacgttt cagaacatgg tctcacttca
    22021 aagatagatg ttcgcttagc aaatggtttg tcagcttttg aagaagcaga taatattgat
    22081 acgatagcta tctgtggaat gggtggtcgt ttgattgctg atattttgaa taacgatata
    22141 gacaaacttc agcacgttaa aacactagtc ttacaaccta ataatcggga agatgattta
    22201 agaaaatggt tagctgcaaa tgattttgaa atagtagcag aagatatctt gactgaaaat
    22261 gacaaatgct atgaaatttt agttgttaag catggtcata tgaatttaac tgctaaagaa
    22321 ttgcgatttg gtccattttt attatccaat aacacaacag tttttaaaga aaaatggcaa
    22381 aatgagctta ataaattaac ttttgcttta aactctattc ccaatagtaa aatggaagaa
    22441 cgtgctattt tggaagataa aatacaggat ataaaggagg ttcttgatga aagctagaga
    22501 acttattgat gtatatgaaa cttattgtcc acaggaatta tctatggaag gagatatttc
    22561 aggtcttcaa attggatctt tagataaaga aataaagaca gtcatggtag ctttagatgt
    22621 tcgagagact acagtagctg aagcaattga acgtcaagta gatcttctta ttgtaaagca
    22681 tgctcctatt tttcgtccat taaaggattt ggtagcaaca ccgcaaaata agatttatat
    22741 agaccttctt aaatcagaca ttgctgttta tgtgagccat actaatatag atatcgttcc
    22801 taatggactt aacgactggt tttgtgaatt attagatatt caatatcccg atatattatc
    22861 tgagacaagt aatggttatg gtattggtcg cattggtgat gttagacccc agagttttga
    22921 attttttgca tggaaaatta aagatgtgtt tggtttagat agcgttcgtt tagtttcata
    22981 tgataaatct aatcctgaaa ttcaacgagt tgctatttgt gggggtagtg ggcaaagttt
    23041 ctacaaagaa gctattgcaa aaggagcaga tgtttttgta acgggagata tttattatca
    23101 tactgcacaa gaaatgatca caaatggttt gcttgcgatt gacccaggcc atcatattga
    23161 ggttttattt gtttctaaaa tagctactat gattgagcag tggaaacttg aaaaaggctg
    23221 ggatatttca gttttagaga gtaaagctcc aacaaatccc ttttaccata tgtaggagct
    23281 attatgaaaa agatagcaat tattggtgca ggagcagtcg gagcgacact agcctattac
    23341 ttatcaaaag aaaaggatat tcaggttact gtttttgatt atggagttgg acaagcaact
    23401 aaggctgctg cggggattat tagcccatgg ttttcaaaac gtcgtaataa agcctggtat
    23461 cgcatggcga ggttgggggc tgatttttat tcaaagctag tcacggattt acaaaaagat
    23521 ggttttgaaa caaaatttta tcagcaaact ggtgttttct tattaaaaaa ggacgagagt
    23581 cagttagagt cattgtttgc actagcagat aaacgtcgac tagaatctcc tctaataggt
    23641 gatttacaaa tactaaataa aagtgaagca aatactcact ttcctgagtt agatggctat
    23701 gaacagttac tttatgcgag tggaggagcg cgagtagaag gggcggactt aacacgaata
    23761 ttgttagaag caagtggtgt taatgtcatc aaggatgaag ttcactttac gataactgat
    23821 aatggctttc gtgttcaagg aattgatttt gataaacttg ttttagcgag tggagcctgg
    23881 ctagctaaga tattagatga gcacaactat caggtagata ttcgccctca aaaagggcaa
    23941 ttgcgagact attattttag caatattaat acaggaaaat atcctgttgt aatgccagaa
    24001 ggcgagctag atattatccc atttgataat ggtaaagtct ctgtaggtgc aagtcacgaa
    24061 aatgatatgg cttttgattt aaacattgat tttaaagtct tagacaagtt tgaagagcaa
    24121 gctattggct attttccgca attaaaaaaa gcagatacga cttctgaacg tgttggaatt
    24181 cgtgcctata caagtgattt ttctccattt tttggccctg tcccttgtat ggagggtgca
    24241 tatgctgcaa gtggattagg atcaactggc ttaacagtgg gcccattaat tggttatgaa
    24301 ttatgtcaat taatcttaaa taaagaaaat cagctaaatt tagaggatta cgatattacg
    24361 aaatacgtga aaaagattag tagatgatag cgtttaaaaa aagtgttata atatgtagga
    24421 ttaatataaa taaggagacc ttactatgaa aggtattatt ttagcgggag gttcaggtac
    24481 tcgcttgtac ccattaactc gtgctgcatc aaaacaattg atgccaattt atgataaacc
    24541 aatgatttat tatccattgt cagttttgat gttggcagga ataaaagaaa tactgattat
    24601 ttctactcca caagatttgc ctcgctttga agatatgctt ggagatggtt cagaactagg
    24661 aatctctctt tcttatgctg aacaaccaag cccagatggg ttagcacagg ccttcattat
    24721 cggtgaagaa tttattggcg atgatcacgt tgccttagta ttaggtgata atatttacca
    24781 tggaccaggt ttatctgcaa tgctacaacg tgcagctagt aaagaaagtg gagcaaccgt
    24841 ctttggctat caagtaaaag atccagaacg ttttggtgtt gtcgagtttg atactgatat
    24901 gaatgccatt tctattgaag aaaaaccagc gcaacctaag tctaattatg ctgtcacagg
    24961 tctttatttc tatgataacg atgtggttga aatagctaaa aatattaagc caagtcctcg
    25021 tggagaactt gaaattactg atgttaacaa agcctacctt gatagaggtg atttatcagt
    25081 tgagttaatg ggacgtggat ttgcgtggtt agacactgga actcacgaaa gtcttcttga
    25141 agcagcacaa tatattgaaa cagtacaacg catgcaaaac gttcaagttg ctaaccttga
    25201 ggaaattgct taccgcatgg gctacatcac tcgtgaacaa gtccttgagt tggcacaacc
    25261 acttaagaaa aatgagtatg gtcaatacct acttcgtttg attggagaag cctaatgact
    25321 gaacaatttt ttgataaaga attaacttgt cgcccaattg aagccattcc tggattgttg
    25381 gaattcgata ttaccgttcg tggagacaac cgtggatggt tcaaagagaa ctttcaaaaa
    25441 gaaaaaatga taccgcttgg tttcccagaa agcttctttg aggcagacaa actacaaaat
    25501 aatatttcgt ttaacaaaaa aaatactttg cgaggtctcc atgcagagcc ttgggataaa
    25561 tatgtttcga tcgctgatga aggacgtgtg atcggtactt gggttgacct ccgtgaaggt
    25621 gacagttttg gtaacgttta ccaaacgatt atcgatgcct caaaaggtat ttttgttcca
    25681 cgcggcgttg ctaatggttt ccaagttctt tcagataaag cagcttatac ttatctcgtt
    25741 aacgattatt gggcacttga actcaaacca aaatatgctt tcgttaacta tgcagatcca
    25801 aatctaggca ttcagtggga aaaccttgaa gaagcagaag tctcagaagc agataagaat
    25861 cacccacttc tcaaagatgt aaaacctttg aagaaggaag atttgtaatt ttcaacttct
    25921 taaaaggcat taaccttagc aagtaaaaac gaattagcaa tgttgtttga cgcaagggtg
    25981 aaaggaaaca tgcaaaaggc ctgttccctc agggatttga aatattgatt tcttaggcat
    26041 gacattccat agaactactt ctaacagaac agtatccttc aaacagcaaa aagaaaaaat
    26101 ttttcggaga aaaaatgact gaatttaaaa acattatcgt aactggtgga gctggtttca
    26161 tcggatcaaa ctttgtacac tatgtttaca ataaccaccc agatgttcat gtaactgtcc
    26221 ttgataaatt gacatatgca ggtaaccgtg ctaacattga agccattctt ggtgatcgtg
    26281 ttgagttagt tgttggtgac atcgctgacg ctgaattggt agacaaattg gctgccaaag
    26341 cggatgctat tgttcactat gcggctgaga gtcacaacga taactcattg gaagatccaa
    26401 gtccatttat ccatacaaac tttatcggaa cttacacttt gcttgaagca gctcgtaaat
    26461 acgatatccg tttccaccac gtgtcaactg atgaagttta tggagatctt ccacttcgtg
    26521 aagaccttcc aggaaatggt gaaggaccag gtgaaaaatt cactgctgaa acaaaataca
    26581 atccatcatc accttactca tcaactaagg cagcttctga ccttatcgtt aaggcatggg
    26641 tacgttcctt cggcgtaaaa gcgaccattt cgaactgttc aaataactat ggaccatacc
    26701 aacacatcga aaaattcatt ccacgtcaaa ttacaaatat cttggcagga atcaaaccaa
    26761 aactttatgg tgaaggtaaa aacgtccgtg actggatcca cacaaatgac cattcaacag
    26821 gtgtctgggc tattttgact aagggtcgta ttggtgaaac ataccttatt ggtgccgacg
    26881 gtgagaaaaa caacaaggag gttcttgaac ttatccttga gaaaatgggt caaccaaaag
    26941 acgcttacga tcacgtaact gaccgtgctg gtcatgatct tcgttacgct atcgattcta
    27001 caaaattgcg tgaagaactc ggctgggaac cacaatttac aaacttttca gaaggtttgg
    27061 aagaaactat taaatggtat acagaaaatg agacatggtg gaaagcagaa aaagatgctg
    27121 tagaagccaa gtatgctaaa actcaagaag tcattaagta attgaaccaa aaaacctatc
    27181 attttgatga taggtttttt atgtacggct agttagatag ctaattggtc tgtttttgtc
    27241 atggtttgag gttggtaata gacattttga tagtcattac caactttttg tactaggtaa
    27301 agtcccgctt tattcatttt gaattgattg ttaatgagac tctcttgatt atcgtgtttg
    27361 ataacagccc aagtttgact gtttttatca tagataactt gttgttttga actattttct
    27421 agtaattcta cttctttgct gttggctaat ttatcaaaac ttgtgcgatc aatgtttgga
    27481 accatcatgt aaccatagct atcacctttg ttgtcatgct tttggcttat agtgataaaa
    27541 ggattgctaa cgattgaggt atttttagaa gtacgattaa tgctgttcca agtacctgtt
    27601 tgctctttgc gttcaatatc aatagtgcta tttttaaaga agatataacc aatattgcga
    27661 ccaggttctt ttgattctaa aaagacactt tttgtatctg taaattgttg agaacttgct
    27721 tgttttaaat caatagtttt tccattgacg tatgtagtat aaggtgtttt agaatcgtct
    27781 tttcgttgat caattgttgt agaaacattt ccaatgccgt tagtattctt gatgttgcta
    27841 cctaaaaaga caatcttatc atttaagata acccaacctt tttgtgctgt taaggtgcga
    27901 tcccagttag taaaatccat agcggcaaga gcaaagtgat cattaagttt gacggaacca
    27961 acaaagtcag atgttcctgt aacttgaccg gttttttctt tagcgtcttt gctatgtttg
    28021 ctcatgaatt ccttagtggt atcttcacgc ttagcatctt tttcagttgt tccagccatt
    28081 ttataaggat tgacggttgg ccaaaaatga ttactataat gagattgatc actattataa
    28141 atatagaaca taccatctcc ggtataccaa ccacgtgtat tttcatcatt cattccttca
    28201 tagttgaggg tacgtttaga atgtaatgaa agcgcgaaac caaagtcttt cttggcatta
    28261 taataagcta agcggtccat gctattaaag gttgataaat tactttttaa aggtttagta
    28321 gcgactgtac tgtcattaag cagcttattc atgttggcaa tatcggaata cgatttgagg
    28381 ttattgaaga cattgtagaa tttatttgaa gtgataatcg ttttaatagt tgatttgagg
    28441 tctaagtttc gctcttcatt agacatgtta gccaatctga ggaaacctct gagaacttca
    28501 actgcagccg catgcgaaga agctgcctct ctactaattg agcgtccacg actcatatcc
    28561 attaactcac cttttacaat taaaggtaaa aatgattgat taatccattt ataaaccata
    28621 tcaagttctt gattagagat tttatagtca gtttcttgaa tgataggcag caattgtgtc
    28681 aaaccatcta tcagaacatt accataggcg ccagtataag caacatttgt atggtcgatg
    28741 taagaaccgt cagcatagaa gccttcagct ttagtagcag tagtaaaaag attttttaga
    28801 gaatgagaag ttttttcgat aatagtattg tctttacgaa gtaaaccttc aatgatttta
    28861 acgcgcccca tatctactag attaccacca agggctttaa atggattgac aagcgtttta
    28921 cggaaatatt ctgcatcagg aacaaagtgt tcaattgggt cggtataagt ttttatttca
    28981 gcgtcagtga agtagttatt catgagagat agggtacctg taattgagcg aggaacaccg
    29041 atttcaaaat cccaccaatt ggcagagcct tctatatctt tattaacatt gtagaagttt
    29101 tgatgaagcc aagccagact ctcttttaca gtacgaatag ctttttcatt tttgtaaata
    29161 gtagagtgtg gattggtgat ttgtttagct aaatcttcca aacgacgata agtagcggtt
    29221 aactgtgctg aattattgag attatctaaa tctttccaaa ggaaagtgcg attagaatcc
    29281 agtttgatag cttcgatgtt tttggcgtta gtttcatcta atttctgatt aagcttttgc
    29341 atgttcgaat cattagtatc gtaaacataa ttaccaatag tcacgtcgtt ccatttgtcg
    29401 aggagttttg taaagttatc ttctgttgaa gctgtaactg ataacggtac ttctttaatt
    29461 attttgccag atttatcaga tattttaaca gttgttgaac cagtagcgtt tggatacaat
    29521 attccaccaa caattttccc aagagacgga ttggttagag tatattggta gtcagcttta
    29581 ttaaaaacgt aatttttgtt taaagccgta ttaacgcttt tttcaatttg tgttgtgacg
    29641 ggttgtggat gctctgagtc tttagggcct tttgttttca ttgatatatt atcaaaagta
    29701 acaaaaccag ttcctttttc ataataaagt tcaagtgtca cctcggagac atttaacttt
    29761 gggttatata tctttgttaa ggtatgtttg ttagtagtac cgctggtcat ctcagaaagc
    29821 caaagtcgcg tattgttgtc ttttttctcc ataatacgaa ggaaagcttg tccagccttg
    29881 ttacttgttt caatatcaaa cttaacctca tattttttag tgggatcaat gttcactttt
    29941 tgtgttacag cacctctaaa gcctttgtcg ctggatagac ttacttggcc ttgttcggta
    30001 cgttggataa taggtgattt atcagcagta tttcctttat caatccaagc agaccattcc
    30061 ctagcgcttg tatgtgacca gcttccagat acagggttag tttgattaaa atctccgttt
    30121 ttcaccagct ccttattttg agtttgatta tctactactt gtttcattgt gctatgagtg
    30181 gctgttgcag ttgcagttga agctgtgggg ttagacagat cttgttgtgt ggttgtggtg
    30241 ctagtctcct tgacagtggt actttcagtc tggagaactt caatattatt agtttgagta
    30301 tctcttattt gggacgttga ggtagttttg gtaagctctt cagctttagc ttggtaacta
    30361 ttgttaacca atattgttcc taaaataagg gctgaataaa gcataatacg atgtttcttt
    30421 ttgatttcca tttttactcc ttagggttta aaattgtaaa ccggtttttc ctaattatag
    30481 catgctttaa aggtttatga taaagaacct ttaaaatgtc atcaaagtga aaagtaaata
    30541 actggtaaac aaaataattt gtttaatcat ttgtgtgcta ctttgatagt aaattgccta
    30601 aataaaggct attttttgtt ataatatgag aatgagagga gtttgaaatg actaaattag
    30661 caacaatttg ttacattgac aatggcaagg aattacttct attgcatcgc aacaaaaaag
    30721 agaatgatgt tcatgaggga aagtggatct cggtaggcgg taaactggag gcaggtgaaa
    30781 ctccggatga atgtgctaag cgtgaaattc tagaagaaac ccatttaaca gtaaaaaaaa
    30841 tggattttaa aggagtcatc acttttcctg aatttacgcc aggtcatgat tggtatacct
    30901 atgtctttaa agtaacagat tatgaaggag agttaatttc agatgatgaa tcacgagaag
    30961 gaaccttaga atgggtacca tatgatcaag ttctctctaa gccaacgtgg caaggagatt
    31021 acgaaatttt taaatggatt ttagaagatg ttcctttctt ttctgctaag tttgtttatg
    31081 atgaacacca aaatttaatt gaaaagacgg ttaattttta tgaaaaatag agattttagg
    31141 gttagagacc agctcgaaac attcggagtc agagtttctg ctttaatcat agaaaatcaa
    31201 aaactgctat taatatatgc tcctcatctc tataaatact atttgccagg tggggcgctt
    31261 caagttggag aagatagcaa taaggctgtt gcgagagaag tacttgagga aataggtctt
    31321 cacagccaag ttggtgattt agcctatatt gttgagaatc agtttaatat taagagacat
    31381 cattatcact cagttgaatt tttatatttt gttaatctct tgggtcaggc gccagaatct
    31441 atcaaagaag gaacgcataa gcgacatttt gtatggcttc caataaaaga attaacaaaa
    31501 attgattgta atcctaattt tttagcacag gatttgatag aatggccagg tcatgttgtg
    31561 cataaaatcg ttcaaaatta actttttact ctcttgt
//
LOCUS       DATROD010000012        29341 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.012, whole genome shotgun sequence.
ACCESSION   DATROD010000012 DATROD010000000
VERSION     DATROD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29341
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.012"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(2..199)
                     /locus_tag="VBC74_000566"
     CDS             complement(2..199)
                     /locus_tag="VBC74_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941539.1"
                     /translation="MKKRLQLLLLIFISAVSWFISNQLATKNTVLSVLIGLVAVIGIT
                     WLIVSLLYYLIVKLIAVIFHD"
     gene            complement(213..1946)
                     /locus_tag="VBC74_000567"
     CDS             complement(213..1946)
                     /locus_tag="VBC74_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6941540.1"
                     /translation="MKASHPKKIQKKLVQDLLSKKSLVGMALLGTVVQVCLTVYLPVL
                     IGQAVDVVLSPHSMILLLPIMWKMVAVILANTIIQWINPLLYNRLIFHYVASLRKAVM
                     EKLNLLPIAYLDKRGIGDLISRVTTDTEQLSNGLLMVFNQFFVGLLTILVTIFSMAKI
                     DLLMLFLVLFLTPLSLFLARFIAKKSYHLYQNQTASRGRQTQFIEEMVSQESLIQAFS
                     AQEESSDHFRTINQEYANFSQSAIFYSSTVNPSTRFINSLIYGFLAGIGALRIMSGAF
                     SVGQLITFLNYVNQYTKPFNDISSVLSEMQSALACAERLYSILEESSPNITGTEKLDS
                     STVKGQIDFKNVIFGYNKSKPLLNGINLHIPAGAKVAIVGPTGAGKSTLINLLMRFYE
                     VDGGNILLDGKPITDYEPSQLRQEIGMVLQETWLKSATIHDNIAYANPKASREEVIEA
                     AKAANADFFIKQLPNGYDTYLEDAGDSLSQGQCQLLTIARIFLKLPRILILDEATSSI
                     DTRTEVLVQEAFQMLMKGRTSFIIAHRLSTIQTADIILVMVSGEIVEVGNHSELIAQK
                     GIYYQMQNAQK"
     gene            complement(1947..3668)
                     /locus_tag="VBC74_000568"
     CDS             complement(1947..3668)
                     /locus_tag="VBC74_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6941541.1"
                     /translation="MKRLTYYFKGYIKETIFGPLFKLLEASFELLVPIVIAKMIDETI
                     PRGDRSSLLLQIGLIFFLAAVGVVVAITAQYYSSKAAVGYTRQLTEDLYQKVMSLGKK
                     DRDELGTASLITRLTADTFHIQTGLNQFLRLFLRAPIIVFGAIIMAFSISPSLTIWFL
                     IMVVTLFIIVFVMSRLLNPIYLKIRTSTDYLVKLTRQQLQGVRVIRAFNQVDRESEAF
                     NDINYHYTNLQLKAGRLSSLVTPLTFLVVNITLVVIIWRGNLNIANHLLSQGMLVALI
                     NYLLQILVELLKMTMLVTSLNQSYISAKRIIAVFERPSEIIDDKLEPKYSNKALEVQE
                     MAFSYPNSSEKALSDITFSMNVGETLGIIGGTGSGKSTLVNLLLHIYKVQEGDIDIYH
                     QGKSPDTISNWRSLVRVVPQNAQLFKGTIRSNLSLGLGKVSEEKLWTALEIAQASDFV
                     KEKDGQLDAPVESFGRNFSGGQRQRLTIARALVQDKIPFLILDDATSALDYLTEARLF
                     KAIIKHFNQTNLIIVSQRINSIQNADRILLLDKGKQVGFDNHQSLLAHNKVYKSIYHS
                     QNFKEEE"
     gene            complement(3680..4282)
                     /locus_tag="VBC74_000569"
     CDS             complement(3680..4282)
                     /locus_tag="VBC74_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEO6941542.1"
                     /translation="MFKFLKRLIALIIIIFIGYRLVIIHENVKKVLQYHDLVQNTLAE
                     NGSEANVHLVLSMIYTETKGDAIDVMQSSESISGTTNSITDSHTSIKHGVTLLSQNIS
                     QAKKAKVDVWTAVQAYNFGSSYIDYVADHGGENSIELAKNYSKNVVAPSLGNYNGDTY
                     FYYHPLALISGGKLYKNGGNIYYSREVQFNLYLIKIMELF"
     gene            complement(4284..5261)
                     /locus_tag="VBC74_000570"
     CDS             complement(4284..5261)
                     /locus_tag="VBC74_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEO6941543.1"
                     /translation="MMDFYIKQIIIHQFSPNDTELVLSDTPLTLTPRIDDYFRKKLSK
                     VFSDEAKRGYFGEDNVFMSHLQDDLYVSSCQIAQLWKEEFVISEDQKTNDLVFIQFDK
                     DGMEHFAFLRISLKEQFAHVSENQEQPITITQNNLPSAAQTPDEALVVNKSSKQYYLI
                     EKRIKHNGSFANYFSENLLQVQPEQSVKKSIKMVEQTAQKIAENFNKDDFSFQSKMKS
                     AIYKNLEEEQELSPEKLADQLFDDNLTARLTFVDELKDAIPGSVQVSDIDHSRQIKKL
                     ENQKLSLSNGIELIVPNNVYQDAESVEFIQNPDGTYSILIKNIQDIQNK"
     gene            complement(5266..6522)
                     /locus_tag="VBC74_000571"
     CDS             complement(5266..6522)
                     /locus_tag="VBC74_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEO6941544.1"
                     /translation="MIFDKDNFKEFDQELWQAIHDEEIRQQNNIELIASENVVSKAVM
                     AAQGSVLTNKYAEGYPSHRYYGGTDCVDVVESLAIERAKMLFNAEFANVQPHSGSQAN
                     AAAYMALIEPGDTVLGMDLAAGGHLTHGASVSFSGKTYHFVSYSVDPKTEMLDYDNIL
                     KIAQETQPKLIVAGASAYSRIIDFEKFRQIADAVDAYLMVDMAHIAGLVASGHHPSPI
                     PYAHVTTTTTHKTLRGPRGGLILTNDEAIAKKINSAVFPGLQGGPLEHVIAAKAVALK
                     EALDPSFKIYGEDIIKNAQAMAKVFKEDDDFHLISDGTDNHLFLVDVTKVIENGKKAQ
                     NVLEEVNITLNKNSIPFERLSPFKTSGIRIGTPAITSRGMGVEESRRIAELMIKALKN
                     HENQDVLTEVRQEIKSLTDAFPLYEN"
     gene            complement(6614..7210)
                     /locus_tag="VBC74_000572"
     CDS             complement(6614..7210)
                     /locus_tag="VBC74_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEO6941545.1"
                     /translation="MNDLGQILEDHGAVIMPTETVYGIFAKALSEEAVNHVYELKKRP
                     RDKAMNLNICDFETILKYSKNQPTYLKQLYDAFLPGPLTIILEASQEVPDWINSGLSS
                     VGFRMPKHPVTLDMIANHGPLIGPSANISGCDSGRVFSEIQKQFNHQVLGIEDDKALT
                     GVDSTIIDLSGDRVKILRQGAITQEVLTATIPELIFEN"
     gene            complement(7203..8033)
                     /gene="prmC"
                     /locus_tag="VBC74_000573"
     CDS             complement(7203..8033)
                     /gene="prmC"
                     /locus_tag="VBC74_000573"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEO6941546.1"
                     /translation="MNYAQLIKHYEQLLEACGEEVENFIYVLKDLKQWSTTDYLLNQN
                     SSVSDTDQQLMENIFQQLKKHRSPQYITGKAYFRDLIFFVDERVLIPRPETEELVDLI
                     LSENKVEDCSVLDIGTGSGAIAISLKKERPSWDVLASDISVSALDLAKENANNCDAEV
                     TFIESDVFSNISDKFDIIVSNPPYISYNDKDEVGKNVLASEPHSALFADEEGLAIYRK
                     IIENSREYLQPRGKLYFEIGYKQGDDLRSLLKRYFPNNRCRVLKDIFGKDRMVVLDNE
                     "
     gene            complement(8033..9112)
                     /gene="prfA"
                     /locus_tag="VBC74_000574"
     CDS             complement(8033..9112)
                     /gene="prfA"
                     /locus_tag="VBC74_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEO6941547.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQNISDAEEMIKDASGDAELEEMAKEELKESKAAKEEYEERLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYEI
                     DQKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTQKLEALNK"
     gene            complement(9147..9716)
                     /locus_tag="VBC74_000575"
     CDS             complement(9147..9716)
                     /locus_tag="VBC74_000575"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEO6941548.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     EFGVVSSRIGMRREAVPISDDMDIFSYIQNLPQKPYCVLIDECQFLSKKNVYDLARVV
                     DDLDVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPDI"
     gene            9854..10036
                     /locus_tag="VBC74_000576"
     CDS             9854..10036
                     /locus_tag="VBC74_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019320686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEO6941549.1"
                     /translation="MPFVKIDLFEGRSQEQKNELAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGELKKK"
     gene            10185..11123
                     /locus_tag="VBC74_000577"
     CDS             10185..11123
                     /locus_tag="VBC74_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEO6941550.1"
                     /translation="MQLSHSLRLMGTTIDIQINSKNAQKQIQEVIELLELYKNRFSAN
                     DFNSELMAINNNAGIKPIQVHPDLFELITIGKEHSLARPSNLNIAIGPLVQTWRIGFS
                     DAKLPSPSEVSEAMILSDPTHILLDSKNQSVFLNQIGMKIDLGALAKGYIADKIMTYL
                     KNEMIDSAIINLGGNVLVHGDNPNRSEGYWVIGIQHPKKKRGQNIGAVKIKNQSVVTS
                     GTYERRLIIDDKEYHHIFDRQTGYPIQTEMASISIVSKQSVDCEIWTTRLFGLSIKEA
                     LDILNAVSYIEGIIITKDDRIYLSDGLKHHFQLFYH"
     gene            11141..11743
                     /locus_tag="VBC74_000578"
     CDS             11141..11743
                     /locus_tag="VBC74_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6941551.1"
                     /translation="MKLIGIVGTNSNKSTNRQLLQYMQQHFADKAEIELIEVKDLPLF
                     NKPADKNVPQVILDIAAKIEEADGVIIGTPEYDHSIPSALMSVLAWLSYGIYPLLNKP
                     VMITGASYGTLGSSRAQLQLRQILNAPELKASVLPDEFLLSHSLQAFDKDGNLHDIET
                     SQKLDAIFDDFRLFVKITGKLSNARDLLQKEAENFDWESL"
     gene            11767..13002
                     /locus_tag="VBC74_000579"
     CDS             11767..13002
                     /locus_tag="VBC74_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6941552.1"
                     /translation="MKFVGIVGSNAEQSYNRMLLEFIRKNFKTKFELEVLEIDDIPMF
                     NQVQNWEESFQLRLLNNKITRADGVIIATPEHNHTITAALKSVLEWLSFAVHPLENKP
                     VMIVGASYYDQGTSRAQIHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDDNGNIINPG
                     TVQFLETCLDNFIKYVGVVSSLRKPKAIESEDLYCQGQIDTTITSIDPDDPDWLDKAA
                     EKVNAVEGDAYVKLDNGLLTVNQLNMFLNAMPFELTYADDNNQFLYYNNNHPDPDTML
                     GKRVREQVGNRMATVHGSLPPARMKNVEWVIGTLRNGNQEYVRTVVPGTPPEVINTHN
                     YQAMYYPDGSYAGINEIIFNFKPWLDWYLQTTGQRLVGGTTAAPGASAVDATSSASTS
                     TEDAPVDQATDATSGASEH"
     gene            13101..13889
                     /locus_tag="VBC74_000580"
     CDS             13101..13889
                     /locus_tag="VBC74_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEO6941553.1"
                     /translation="MSLFQEKIAYNCAKKEALYKESLGRYALRSMLAGAYLTMSTAAG
                     IVAADTIGKISPALSGFVFAFIFSFGLIYVLIFNGELATSNMLYLTAGAYNKNISWKK
                     AITILIYCTFFNLVGACILAWLFNQSYSFQHLTNDSFLGHVVAKKLGKPSSGAFLEGI
                     IANMFVNLAILAYMLLKEESAKMTVILSAIFMFVFLSNEHLIANFASFMLAAFSHIEH
                     IKGFTLLNVIRQWTLVFFGNWIGGGVFIGLAYAWLNKTKTSHHE"
     gene            complement(13988..15262)
                     /locus_tag="VBC74_000581"
     CDS             complement(13988..15262)
                     /locus_tag="VBC74_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEO6941554.1"
                     /translation="MKFNEQSNSQAALLGLQHLLAMYAGSILVPIMIASALGYNAKQL
                     TYLIATDIFMCGIATLLQLRLSKHFGVGLPVVLGCAFQSVAPLSIIGAQQGSGYMFGA
                     LIASGIYVVLVAGIFSKVANFFPPIVTGSVITTIGLTLIPVAMGNMGDNAKEPSLQSL
                     TLSLVTIGVVLLINIFAKGFLKSISILIGLISGTILAAFMGLVDASVVAEAPLVHIPK
                     PFYFGAPRFEFTSILMMCIIATVSMVESTGVYLALSDITNDKLDSKRLRNGYRSEGLA
                     VLLSGLFNTFPYTGFSQNVGLVQISGIRTRKPIYFTALFLVILGLLPKFGAMAQMIPS
                     PVLGGAMLVLFGMVALQGMKMLNQVDFEHNEHNFIIAAVSIAAGVGFNETNLFISLPN
                     TLQMFLTNGIVISTLTAVVLNIILNGLPKKLI"
     gene            complement(15262..15843)
                     /locus_tag="VBC74_000582"
     CDS             complement(15262..15843)
                     /locus_tag="VBC74_000582"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEO6941555.1"
                     /translation="MKLLEERILKDGDVLGENILKVDSFLTHQVNFELMQEIGKVFAD
                     KYKEAGITKVVTIEASGIAPAVYAAQALGVPMIFAKKAKNITMTEGILTAEVYSFTKQ
                     VTSQVSIVSRFLSNDDTVLIIDDFLANGQAAKGLLEIIGQAGAKVAGIGIVIEKSFQD
                     GRDLLEKTGVPVTSLARIKAFENGRVVFAEADA"
     regulatory      complement(15947..16042)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(16098..17081)
                     /gene="guaC"
                     /locus_tag="VBC74_000583"
     CDS             complement(16098..17081)
                     /gene="guaC"
                     /locus_tag="VBC74_000583"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEO6941556.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIISSRSQADTSVKLGNYTFKLPVI
                     PANMQTIIDEEVAETLACEGYFYIMHRFNEEARRPFIKRMHDKGLIASISVGVKDYEY
                     DFVTSLKEDAPEFITIDIAHGHSNSVIEMIQHIKQELPGTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCSKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHLESPGKLVEVEGQQFKEYYGSASEYQKGEHKNVEGKKIL
                     LPVKGRLEDTLTEMQQDLQSSISYAGGKELDSLRHVDYVIVKNSIWNGDSI"
     gene            complement(17285..18625)
                     /locus_tag="VBC74_000584"
     CDS             complement(17285..18625)
                     /locus_tag="VBC74_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6941557.1"
                     /translation="MTKKGWRIMPAIITTAILGFSGILIETSMNVTFPLLMKEFGVNP
                     AVIQWVTTGNLLAVAVTVPLSAFMIKNLSERQIFTLANVLFLFGVLIDSFAPNLAILL
                     VGRVLQGVGTGLALPLLFHIILTQIPMERRGLMMGVAAMVTLLAPAVGPTYGGVISGM
                     LGWKMIFMLLAPILIISTFIGLASIPKRQVRINDKLNFPAFVSLGIGLATLLLAIEKM
                     SIFYLLVAIVSFVIFYYLNKQLEFLNLNVFKDKDFSILLYGVLAFQMIPLALSFLLPN
                     LLQLVLHQTSTKAGLFMFPGAIAVVFLSPFAGYLLDKIGAFKPIMIGISLSLIGLIGT
                     AIFIPAKFVVVLLAFDILTKIGMGIGASNMVTTALTKLKPAQSADGNSILNTLQQFAG
                     AFATAVASQIFTIGQVAIPKNGAIIGSQFAVLFVIVVVILAIVGLTYLRKRKAI"
     gene            18917..19588
                     /locus_tag="VBC74_000585"
     CDS             18917..19588
                     /locus_tag="VBC74_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941558.1"
                     /translation="MAFASNESENNFFITLKTYFNYLFSIQIITDISTLNHADFDGSF
                     AFHDIETSIPHLVIDSNYLAISQTNSKTEANDIKTFSELSKTMTEFYYMLNFDLFNHL
                     PYRFRLHNKDGQTIYSNHKPEDPFDIYPEEEYPIDKWVQNSLIEKKAKELHLLLPGAS
                     QDYILVQSYKRLENDSGQLVGYIEHVHNIKPLLEGYLKESGQAIVGWSDVTSGASISN
                     DDFEL"
     gene            complement(19659..21659)
                     /locus_tag="VBC74_000586"
     CDS             complement(19659..21659)
                     /locus_tag="VBC74_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEO6941559.1"
                     /translation="MQHVNHSSFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GISSVTESFILGSISLIIWTLTLITTIKYVLVALKADNHHEGGIFSLYTLVRKMTPWL
                     IVPAVIGGATLLSDGALTPAVTVTSAVEGLKVVPSLQHIFQNQSNVIFATLFILLLLF
                     AIQRFGTGVIGKLFGPIMFIWFAFLGISGLLNSFAHPEVFKAINPYYGLKLLFSPENH
                     KGIFILGSIFLATTGAEALYSDLGHVGRGNIHVSWPFVKVAIILSYCGQGAWILANKN
                     AGNELNPFFASIPSQFTMHVVILATLAAIIASQALISGSFTLVSEAMRLKIFPQFRST
                     YPGDNIGQTYIPVINWFLFAITTSIVLLFKTSAHMEAAYGLAITITMLMTTILLSFFL
                     IQKGVKRGLVLLMMIFFGILEGIFFLASAVKFMHGGYVVVIIAVAIIFIMTIWYKGSK
                     IVSRYVKLLDLKDYIGQLDKLRHDHRYPIYHTNVVYLTNRMEGDMIDKSIMYSILDKR
                     PKKAQVYWFVNIKVTDEPYTAEYKVDMMGTDFIVKVELYLGFKMRQTVSRYLRTIVEE
                     LLESGRLPKQGKTYSVRPDSKVGDFRFIVLDERFSSSQNLKPGERFVMLMKSSVKHWT
                     ATPIRWFGLQFSEVTTEVVPLIFTANRGLPIKEKIELTTTGD"
     gene            complement(21794..22554)
                     /locus_tag="VBC74_000587"
                     /pseudo
     CDS             complement(21794..22554)
                     /locus_tag="VBC74_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649058.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            complement(22616..23608)
                     /gene="pta"
                     /locus_tag="VBC74_000588"
     CDS             complement(22616..23608)
                     /gene="pta"
                     /locus_tag="VBC74_000588"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEO6941560.1"
                     /translation="MRSLFGGLREKVASDYVKIVFPEGSDERVLRAAARLKFEGLVDP
                     VILGKADEVHDSLARLGFVDQDYSVIDPEQYEKFEEMKEAFVEIRKGKATMEDADRLL
                     KDVNYFGVMLVKLGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRENT
                     QERYIFADCAINIDPNAQELAEIAVNTADTAKIFDINPKIAMLSFSTKGSAKAPQAEK
                     VQEAAKIAKDLSPELAVDGELQFDAAFVPETAEIKAPNSDVAGKANVFIFPDLQSGNI
                     GYKIAQRLGMFEAIGPILQGLNAPVNDLSRGSSAEDIYKLAIITAAQAIDEKSK"
     gene            complement(23634..24518)
                     /locus_tag="VBC74_000589"
     CDS             complement(23634..24518)
                     /locus_tag="VBC74_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO6941561.1"
                     /translation="MKFEYIAEERCKVKTLLKSHDVSRGLLAKIKYRGGKIFVNGEEQ
                     NAIFLLEIGDVVTIDIPDEPSHETLEPVPHDLDIVYEDDHFLILNKPFGFASIPSSIH
                     SNTIANFIKHYYVSNNYANQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQAKAIE
                     KRYYALVSGSGDLADSGDIIAPIARDVDSIITRRVHESGKYAHTSYQVVARYGDVRLV
                     DIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRMDLGINRQALHCHSLSFYDPFMGKIN
                     KQTLDLTDDFDSVIMELH"
     gene            complement(24515..25351)
                     /locus_tag="VBC74_000590"
     CDS             complement(24515..25351)
                     /locus_tag="VBC74_000590"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEO6941562.1"
                     /translation="MTQMNFTDRATRVAIIANGKYQSKRVASKLFAAFKHDPDFYLSK
                     KDPDIVISIGGDGMLLSAFHMYEKQLDKVRFVGVHTGHLGFYTDYRDFEVDTLINNLK
                     NDKGEQISYPILKVTITLEDGRVIRARALNESTIKRIEKTMVADVVINQVVFERFRGD
                     GILVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVYRTLGSSVIIPKKDAIE
                     IVPKRVGVYTISIDNKTVHYKNVTKIEYSIDEKSINFVSTPSHTSFWERVNDAFIGEP
                     EH"
     gene            complement(25326..25997)
                     /locus_tag="VBC74_000591"
     CDS             complement(25326..25997)
                     /locus_tag="VBC74_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO6941563.1"
                     /translation="MSMDWETFLDPYIQTVGELKIKLRGIRKQFRKQNRHSPIEFVTG
                     RVKSVESIQEKMVLRGISEENLAQDLQDIAGLRIMVQFVDDVDEVLALLRKRHDMTVV
                     QERDYITHMKSSGYRSYHVVVEYPVDTIDGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPEEIKQRLEKTAKIALELDEEMRKIREDIREAQLLFDPLNRKLSDGVGNSDDT
                     DEFYR"
     gene            26107..26679
                     /locus_tag="VBC74_000592"
     CDS             26107..26679
                     /locus_tag="VBC74_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEO6941564.1"
                     /translation="MTHLEIEYKTLLNKDEFNRLTSLFSHVQPITQTNYYFDTETFEM
                     KAHRMSLRIRTLPNRAELTLKIPREVGNLEHNHDLTLEEAKYIVKNGQFPEDTEIASL
                     ILEKGVDPTKLAVFGQLTTTRREMETSIGLMALDSNIYADIKDYELELEVKQPKQGKR
                     DFDQFLKENNINFKYAKSKVARFSATLKNN"
     gene            26856..27830
                     /locus_tag="VBC74_000593"
     CDS             26856..27830
                     /locus_tag="VBC74_000593"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO6941565.1"
                     /translation="MAEQYADKQIKLFSLTANREIAEKISQASGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVVPYFGYSR
                     QDRIAASREPITAKLVANMLVKAGVDRVLTLDLHAVQVQGFFDIPVDNLFTVPLFAEH
                     YNQLGLSGEDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREEGYIIGEVEG
                     KKAIIIDDILNTGKTFAEAAKILERGGATEIYAVASHGLFAGGAADILESAPIREIIV
                     TDSVLSKERIPSNIKYLTASHLIADAIIRIHERKPLSPLFSYRSDKKD"
     gene            27834..28949
                     /locus_tag="VBC74_000594"
     CDS             27834..28949
                     /locus_tag="VBC74_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6941566.1"
                     /translation="MIYLDNAATTALTPSVIEKMTNVMTSNYGNPSSIHTFGRQANQL
                     LRECRQIIAEYLNVNSREIIFTSGGTESNNTAIKGYALANQLKGKHIITSEIEHHSVL
                     HTMTYLSERFGFDITYLKPNHGQITAKDVQEALRDDTIMVSLMFANNETGDFLPIQEI
                     GQLLRNHQAVFHVDAVQVFSKMELDPHSLGIDFLAASAHKFHGPKGVGILYCAPHHFD
                     SLLHGGDQEEKRRASTENIIGIAGMSQALTDATTNTLKNWTHISQLRTTFLDAISDLD
                     FYLNNGQDCLPHVLNIGFPRQNNGLLLTQLDLAGFAVSTGSACTAGTVEPSHVLTSLY
                     GANSPRLNESIRISFSELNTQEEILELAKTLRKIIGD"
     gene            28951..29298
                     /locus_tag="VBC74_000595"
     CDS             28951..29298
                     /locus_tag="VBC74_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEO6941567.1"
                     /translation="MAFEKEINLPDCNYIYTISPNIKKYTLRDTTFTQNRLGNYELER
                     LLETVPNSGDGFPLKITINKELSGFKLLITDKSGLRIVNIFKSTDHKILQDKFYFLMD
                     SLVERNIFTKTAK"
BASE COUNT         9727 a         5664 c         4588 g         9362 t
ORIGIN      
        1 ttcaatcatg aaatataact gcaattaact tcacgatcaa ataatagagc agacttacaa
       61 ttaaccacgt tatcccaata actgcaacta aacctattaa aacagataat accgtattct
      121 tagttgctag ttggttactg ataaaccatg aaactgcaga aataaatatc agtaataata
      181 gttgaagcct ttttttcata taattcctct tactactttt gtgcattttg catttgataa
      241 taaatacctt tttgagctat taattcagag tggttaccca cttctacaat ttcaccagat
      301 accataacta aaataatatc tgctgtttga atggtagata agcgatgtgc aataataaag
      361 cttgttctcc ccttcatcaa catttggaat gcttcttgaa caagaacttc agtacgagta
      421 tctatagacg atgtcgcctc atctaatatc aatattctag gtaatttaag aaaaatacga
      481 gcaattgtca atagctgaca ttgcccctgt gataacgaat ctccagcatc ttctaaatac
      541 gtatcatatc cattcggtaa ttgtttgata aagaaatctg cattagctgc ttttgcagcc
      601 tctatcactt cttcacggct agctttagga ttggcatatg caatgttatc gtgaatcgta
      661 gcacttttta accacgtttc ttgtaatacc attccaattt cttgccttaa ttgtgatggc
      721 tcataatcag taataggctt accatctaaa aggatatttc ccccgtcaac ttcatagaaa
      781 cgcattaata aattgattaa agtagacttc ccagcacctg ttggtcctac aatagcgacc
      841 ttagctcctg ctggaatgtg taaattaata ccattcaata gtggttttga tttattataa
      901 ccaaaaataa cgtttttaaa atcgatttga cctttgaccg ttgatgaatc aagtttttct
      961 gtacctgtta tatttggtga cgattcttct aaaatagagt aaaggcgttc tgcacaagcc
     1021 agagcacttt gcatctcaga caggacggaa gagatgtcat taaatggctt agtatattga
     1081 ttgacataat ttaagaaagt gattaattga ccaacactaa aagcacctga cataatacga
     1141 agggcaccaa tccctgctaa gaagccataa atcaaactat tgataaatcg ggttgatgga
     1201 ttgacagttg aagaataaaa aattgctgat tgagaaaagt tagcatactc ttgattaata
     1261 gtcctaaaat gatcactact ttcttcttgc gcactgaatg cttgaataag gctttcttga
     1321 ctcaccatct cttcaatgaa ttgagtttgt cgtcctcgtg atgccgtttg attttgatat
     1381 aagtgataac tctttttagc aataaagcgg gctagaaaga gtgataaagg cgtcagaaat
     1441 aataccaaaa aaagcattaa taaatcaatc tttgccatac taaaaatagt aactagaata
     1501 gttaataaac caacaaagaa ctggttaaaa accattaata aaccatttga taattgctcc
     1561 gtatcggtag tcacacgact aatcaagtca cctatgccac gtttatcaag gtaagctata
     1621 ggtaatagat tcaacttttc cattacagcc tttcttaaag aagcaacata atgaaaaatt
     1681 aagcggttat agagcagagg attaatccat tgaataatag tatttgctaa aataactgca
     1741 accatcttcc acattatagg taacaataat atcatagaat gaggcgataa tacaacgtct
     1801 accgcctgtc caattagaac aggcaaataa acagtgaggc aaacttgtac taccgtccct
     1861 aataatgcca ttcctactaa gcttttttta gaaagaagat cttgaacaag ctttttttgt
     1921 atctttttag gatgacttgc tttcatttac tcctcctcct taaaattttg tgaatggtaa
     1981 atggacttat aaactttgtt atgagctaat aaagattgat gattatcaaa accaacttgt
     2041 ttgcccttat caaggagtaa gattctatct gcattttgta tactattaat tctctgtgat
     2101 acaataataa gattagtttg gttgaaatgt ttaattatag ctttaaataa acgagcttct
     2161 gttaaataat ccaatgcaga tgttgcatcg tctaaaatca aaaatggaat cttatcttga
     2221 actaaagctc ttgcaatcgt caacctttgc ctttgaccac cagagaaatt cctaccaaaa
     2281 ctctctacag gggcatcaag ttgaccatct ttttctttta caaaatcact agcttgtgct
     2341 atttctaaag cagtccaaag tttttcctca ctaactttac caagtcccaa agaaaggtta
     2401 gaacgaatag ttcctttaaa caactgagca ttttgaggaa caactcttac caggctacgc
     2461 caatttgaaa ttgtatctgg actttttccc tgatgatata tatcaatatc cccttcttgc
     2521 actttataaa tatgaagcag taaattaacc aaggttgatt tcccagagcc agttccacca
     2581 attatcccta aagtttctcc tacattcata gaaaaagtaa tatcagataa agctttttca
     2641 gaggagtttg gataagaaaa tgccatttct tgcacttcta aagccttatt cgaatatttt
     2701 ggctcaagtt tgtcatcaat tatttcagac ggtctttcaa aaacggctat aattcgctta
     2761 gcactgatat aactttggtt cagtgatgtc accaacattg tcatttttaa caattcaaca
     2821 agaatttgca ataagtaatt aattaaagca accaacattc cttgtgataa aagatgatta
     2881 gctatattta aattaccacg ccaaataatc acaaccaaag tgatattaac aactagaaat
     2941 gttagaggcg ttactaaact agaaagccta cctgctttta attgtaaatt cgtatagtga
     3001 taattgatat cattaaatgc ttcactctct ctatctactt gattaaaggc acggataact
     3061 cgtacacctt gaagttgttg cctagtcaac tttaccaaat aatcggttga agttctgatt
     3121 ttaaggtaaa taggatttaa tagtcgagac attacaaaaa caatgataaa taacgtgaca
     3181 accattatca agaaccaaat agtcaatgag ggactaatgg aaaaagccat aattatggca
     3241 ccaaaaacaa taatcggagc cctcaaaaat aaacgtaaaa attgatttaa accagtttgg
     3301 atgtgaaatg tatcagcagt caaacgagta atcaaactag cagttcccaa ttcatctctg
     3361 tcttttttac ccagtgacat gactttttga tagagatcct ctgtcaattg tctcgtataa
     3421 ccaactgcgg cttttgaaga ataatattga gcagttatcg ctactacaac acccaccgca
     3481 gccaaaaaga aaatcaatcc aatttgcaac agtaaactac ttctatcccc tcgtggaatg
     3541 gtctcgtcaa tcatttttgc aataacgatt ggtaccaaaa gttcaaaaga ggcttctaat
     3601 aatttgaaaa ggggtccaaa gatagtttct ttgatatacc ccttaaaata ataagttagt
     3661 cttttcatac ttacccttct taaaacaact ccattatttt tatcaaataa aggttgaatt
     3721 gaacttcacg tgagtagtaa atattaccac catttttata gagcttccct cctgaaatta
     3781 aggcgagagg atgatagtaa aagtaagtgt ccccgttata atttcctagg ctgggagcta
     3841 caacattttt agagtaattt ttggccaact caatggaatt ttcaccccca tgatccgcta
     3901 cataatcaat ataagaagaa ccaaaattat atgcttggac agcagtccat acatcaacct
     3961 tagctttttt agcttgactt atattttgtg acaacagagt gacgccatgt ttaatactgg
     4021 tgtgactatc cgtaatactg ttcgtagttc cactgatact ttcacttgat tgcatcacat
     4081 ctatagcatc tcccttagtt tcagtataaa tcatagataa tactaagtgc acattagcct
     4141 ctgaaccatt ctctgcaaga gtattctgca ctaaatcatg atattgaagc acttttttaa
     4201 cattttcatg gatgataact agacggtacc caatgaaaat aataataatt aatgctatca
     4261 aacgttttaa aaatttaaac atattattta ttttgaatat cctgtatgtt tttgattagt
     4321 attgagtatg taccatcagg gttttggata aattcaactg attcggcatc ttggtagaca
     4381 ttgttgggta caatcaattc aataccattt gaaagagata gcttctgatt ctctaacttc
     4441 ttaatttgac gggaatgatc aatatcactt acttgaacag atccaggtat tgcgtctttc
     4501 aattcatcaa caaaagtcag cctggctgtt aaattatcat caaataattg atctgctaat
     4561 ttttctggag acaattcttg ctcttcttct aaattcttat aaatagccga tttcattttt
     4621 gattggaaag aaaaatcatc tttgttgaaa ttttcagcaa ttttttgagc tgtctgttca
     4681 accattttta tagatttttt aacagactgt tctggttgta cttgtaataa attttcagaa
     4741 aaataattag caaatgaacc attgtgtttg atacgctttt caattaaata gtattgcttt
     4801 gatgacttgt taacaaccaa agcttcatct ggagtttgag cagcacttgg aaggttattc
     4861 tgtgttattg taattggctg ctcttgattt tcagaaacat gagcaaattg ttcctttaat
     4921 gagattcgaa ggaaagcaaa atgttccatt ccatccttgt caaattgaat aaaaactaag
     4981 tcattcgttt tttgatcttc cgatatgaca aactcttctt tccaaagttg cgcaatttga
     5041 caggaagaaa catataaatc atcttgtaaa tgactcataa atacattatc ctctccaaaa
     5101 tagccacgct tagcttcatc agaaaaaact ttacttaatt ttttacgaaa gtagtcatca
     5161 atccttggag ttagggttaa cggagtatct gatagcacta actctgtgtc gttaggagaa
     5221 aactgatgta taataatctg tttgatataa aaatccatca taacattaat tctcatacaa
     5281 agggaaagcg tccgttaatg atttaatttc ttgacgaact tctgtgagga catcttgatt
     5341 ttcatgattt ttgagtgcct taatcattaa ttcagcgatt cttcgagatt cttcaactcc
     5401 cattcctcga ctagtaattg ctggagtacc aattcgaata cctgatgttt taaatggtga
     5461 tagtcgctca aacggtatag agttcttgtt aagggtgata ttgacttctt caagaacatt
     5521 ttgtgccttt ttgccattct caataacttt tgtgacgtct actaagaaca aatgattatc
     5581 agtaccatca gaaatcaaat gaaagtcatc atcttcttta aaaactttag ccatggcttg
     5641 agcattttta ataatatcct caccgtatat tttaaaggaa ggatctagag cttctttcaa
     5701 agctacagct tttgcagcaa ttacatgttc taatggtccg ccttggagtc ctggaaagac
     5761 tgcagaatta atttttttag caattgcttc atcattggtt aaaatcaaac cacctctagg
     5821 tccacgtaga gtcttatgtg ttgtcgttgt cgttacatga gcataaggaa taggactagg
     5881 atgatgacca gaagcaacta aacctgcaat gtgagccatg tcaaccatta agtaagcatc
     5941 tactgcgtct gctatttgac gaaacttttc aaaatcaata atgcgagaat aggccgacgc
     6001 ccctgcaaca attaatttag gttgcgtttc ctgtgctatt ttcaaaatgt tatcatagtc
     6061 tagcatctcc gtcttaggat caacagagta agaaacaaaa tgataagttt ttcctgagaa
     6121 gctaactgaa gcgccatgtg ttaagtgccc acctgctgct aagtccatcc ctaagacagt
     6181 atctcctggc tcgattaaag ccatgtaagc tgctgcatta gcctgactac cagaatgtgg
     6241 ttgaacatta gcaaactcag cattaaataa catcttagct cgttctatag ctaaactttc
     6301 aactacatca acacaatctg ttcctccata ataacgatga ctaggatacc cttcggcata
     6361 tttatttgtc aaaacagaac cttgtgctgc cataacagct tttgaaacaa cgttttctga
     6421 tgcaattaat tcaatattat tttgttggcg aatttcctca tcatgaatag cctgccaaag
     6481 ttcttggtca aattctttaa aattgtcttt atcaaaaatc attttttact ccttaagctc
     6541 gttataactt ttaaataatg gtttcaatca ttatatttat cacattttta ctattagttg
     6601 caagatgatt aaattaattt tcaaaaatta attcaggtat cgtagccgtt agtacctctt
     6661 gcgtgattgc accttgcctt aaaatcttaa ctctatcacc agacaaatca ataatagtag
     6721 aatccacacc tgtcaatgct ttatcatctt caatacctaa aacttgatga ttaaactgtt
     6781 tttgaatttc tgagaataca cgaccactat cacatccaga aatattagca gaaggaccaa
     6841 tcaatggacc atggttagca atcatatcaa gggttacagg gtgcttaggc atccgaaatc
     6901 ctacgcttga taacccagaa ttgatccaat ccggaacttc ttgtgaagct tccaaaataa
     6961 tggttagcgg tcctggtaaa aaagcatcgt acaattgctt caaataggtc ggttgatttt
     7021 tagaatattt taaaatagtc tcaaaatcac atatatttaa gttcatagcc ttatcacgag
     7081 gtcttttttt aagctcatag acgtggttca cagcttcttc actcaaagct ttggcaaaga
     7141 ttccgtagac cgtttctgtt ggcataataa ctgcgccatg atcctctaaa atctgtccca
     7201 aatcattcat tatctaatac caccattcta tctttaccaa atatatcttt caaaactctg
     7261 caacgattgt ttggaaaata tcttttcaat aaacttcgta aatcatcacc ttgtttgtaa
     7321 cctatttcaa aatataattt acctctaggt tgcaaatatt ctcttgagtt ttcaatgatt
     7381 ttacgataaa tagctaatcc ttcttcgtct gcaaaaaggg cactatgagg ctctgaagct
     7441 agcacattct tccccacctc gtctttgtca ttgtaagaaa tatagggagg attagacaca
     7501 ataatatcaa atttatctga aatgttacta aagacatctg attctataaa agtcacttcc
     7561 gcatcacaat tattagcatt ttcctttgcc aagtctaatg cagatacaga aatgtcacta
     7621 gctagaacat cccaagaggg gcgttctttt tttagagaaa tggcaattgc tccactacct
     7681 gtaccaatat ctaaaacaga acaatcttct actttattct ctgataagat tagatcaacc
     7741 aactcctctg tctcaggcct cggaattaaa actcgttcat caacaaagaa aataagatct
     7801 ctaaagtaag ctttacctgt aatatactgc ggtgatctat gttttttaag ctgttgaaaa
     7861 atattttcca ttaattgctg atcagtatca ctcacactac tattttgatt taaaaggtaa
     7921 tcagttgttg accattgttt taggtctttt aatacataaa taaaattttc gacttcttca
     7981 ccacaagcct ctaacaactg ttcataatgc ttgattaact gcgcataatt cattatttat
     8041 ttaaggcttc taacttttga gtctgatcat acataacaag tgcatcaata acttcatcca
     8101 tttttccaga taaaatagta tctaattttt gaagtgtcaa accaatacga tgatctgtta
     8161 cacgattttg tgggaaatta taagtgcgaa tacgttctga acgatcacca gtaccaactg
     8221 ttgatttacg ttcagcatct tgttcatctt gagcaatttg cgcaaaatgg tcagcaacac
     8281 gagcacgaat gattttcatc gctttatcac ggtttttttg ctgagtacgc tcttcttgca
     8341 tttcaacttt gattcctgta ggaatgtgta ccatacgaac tgcagtagca actttattga
     8401 cgttctgacc accagcacca gacgcgtggt aaatatcgac gcgaagatct ttttgatcaa
     8461 tttcgtattc tacttcttca acttctggca ttaccaaaac agttgcagtt gaagtatgaa
     8521 cacgaccctg actctctgtc accggaacac gttgaacacg atgagctcct gattcatatt
     8581 taagcttaga ataaactgac tgaccagaaa ccatagcaac cacttcttta ataccaccaa
     8641 caccattaac ggatgattcc ataacttcaa aacgccatcc ttgagtttca gcatattttt
     8701 gatacattgt taaaagatca ccagcaaaaa gcgctgcttc atcgccacca gcagcaccac
     8761 gaatttctaa aataatgttt ttatcatcgt taggatcttt aggtaataga aggattttta
     8821 atctttcttc atactcttct ttagctgctt ttgattcttt aagttcttct ttagccattt
     8881 cttccaattc agcatcacca gaagcatcct tgatcatttc ttcagcatct gaaatatttt
     8941 gaatgacttg cttatattct ctataagctg tgacagtttc tcgagtatta gcttcttcac
     9001 gtgataattc cataaatcgc tttgtatctg aaacaacatc tggatcacta agtaactcac
     9061 ctaactcttc ataacgatct tcaactgcct gcaattgatc ataaatattc atttttcttt
     9121 gtatctcctt taaaaccgat tatcttttaa atatctggat taaaataatg ttttcgacaa
     9181 actgggatat atgtttcatt accaccaatt tgtatctgat caccttcata gacaggtttt
     9241 ccgttttctg tccttaaaac catggttgct ttcttactac agtattggca aattgttttg
     9301 atttcatcaa tcttatcggc caataatagc aaatgttttg aaccttcaaa aagattgttt
     9361 tggaaatcat ttttcaaacc aaaagccatg actggtacat ctaagtcatc tactactcta
     9421 gctaaatcgt agacgttttt cttactcaaa aattgacatt catcaattaa aacacaataa
     9481 ggcttttgtg gtaaattctg aatataggag aaaatgtcca tatcatctga aattggaact
     9541 gcttcacgac gcattccaat acgacttgaa acgactccaa attcatctcg agtgtccaag
     9601 gcgcttgtca taataaccac aggttttcct tgttcttcat agttatgagc aacttttaaa
     9661 atttcaatgg ttttaccaga attcattgtc ccatatttat aatagagttg agccaaagcg
     9721 gaaaatccta ctttctatag tattctacct attttaccac aaaggctgaa gttttcccag
     9781 ccattgaaaa cagtgtgtac tttttcacaa cattatgtta caatgatgcc caataacata
     9841 aaggagtaat attatgccat ttgtaaaaat tgacttgttt gagggacgct cacaagaaca
     9901 aaaaaatgaa ttagctcgcg aggttactga agtagtatca cgcattgcaa aagcacctaa
     9961 agaaaatatc catgttttta tcaacgatat gcctgaagga acctactatc ctcaaggtga
    10021 acttaagaaa aaataagaaa tagtaaaaga ggttacccag gtaacctctt ttgatagtct
    10081 ttgcaagcat atttcttgac aaattgaaaa tatttgaaaa taagtattga ttttcaagtt
    10141 agaaaacggt atcattagaa tatattgctt tgggggtgga aaatttgcaa ctttctcata
    10201 gtttaagact catgggaacg actatcgata ttcaaattaa ttctaaaaat gctcaaaaac
    10261 aaatccaaga ggtcatagaa ctcttggagc tctacaaaaa tcgctttagt gcaaatgact
    10321 ttaattcaga gttaatggct ataaataaca acgctggtat caagcctatt caagtacatc
    10381 cagacctctt tgaattaatc acaattggga aagaacacag cttagctagg cctagtaacc
    10441 taaatattgc tattggtcct ttagtgcaaa cttggagaat tggattttca gatgctaaac
    10501 tacctagccc tagtgaagta tcagaagcta tgattttatc tgacccaact catatacttc
    10561 tagattcaaa aaatcaatct gtttttttaa atcagatagg aatgaaaatt gatttgggtg
    10621 cacttgcaaa aggatatatt gctgacaaaa ttatgaccta tctcaaaaat gagatgattg
    10681 attctgctat cattaattta ggcggaaacg ttttagttca cggagataat cctaatcgtt
    10741 cagaagggta ttgggttatc ggcattcaac atccgaaaaa gaaacgggga caaaatattg
    10801 gagctgtcaa aattaaaaac caatctgttg taacctcagg tacttatgaa cgccgtctga
    10861 ttattgacga taaggaatac catcatattt ttgatcgaca gactggttat ccgatacaaa
    10921 cagagatggc tagcatttct attgtttcta aacaatccgt cgactgtgaa atctggacca
    10981 caaggttgtt tggcttatcc attaaagagg ctcttgatat tttaaacgct gtttcctaca
    11041 ttgaaggcat tatcatcact aaagatgacc gtatctattt atctgatggc ttaaaacatc
    11101 attttcaact attttatcat taagaaagag gatttctaat atgaaactta ttggcatcgt
    11161 tggaactaac tcaaataaat caacaaatcg tcaattatta caatacatgc aacaacactt
    11221 tgctgacaaa gcagaaattg aactcatcga agttaaagac cttccgttat ttaataaacc
    11281 tgctgataaa aatgttcccc aagtaatttt agatattgct gctaaaattg aagaagctga
    11341 tggtgttatt attggtacgc cagaatatga tcattctata ccttcggcct taatgagtgt
    11401 tttagcttgg ctatcatatg gtatttaccc acttctcaac aaacccgtta tgattactgg
    11461 tgcttcatat ggaacacttg gatcatcacg tgcacaatta caattacgtc aaatacttaa
    11521 cgctcctgaa ctaaaagcaa gtgttttacc tgacgaattc ttgttatcac actctctcca
    11581 agcctttgat aaagatggca acctacacga tatcgaaaca agtcagaagc tagatgctat
    11641 ctttgatgat tttcgtcttt ttgttaagat tactggtaaa ttatcaaatg ctcgtgattt
    11701 acttcaaaaa gaagcagaaa attttgactg ggaaagttta taagagtata gaagggaata
    11761 actaagatga aatttgtagg tattgttggc tcaaatgcag agcaatcata taatcgtatg
    11821 ttgcttgaat ttattcgcaa gaattttaaa accaaatttg aattggaagt ccttgaaatc
    11881 gatgatattc caatgtttaa tcaagtccaa aattgggaag aaagtttcca acttcgtctt
    11941 cttaataata agatcacacg agctgatggc gttattattg ccactcctga acataatcat
    12001 actattacgg ccgctcttaa aagtgtgctt gagtggctct catttgctgt gcatccactc
    12061 gaaaataaac ctgttatgat tgtcggtgct tcatattatg atcaaggcac atcacgtgct
    12121 caaatacacc ttcgtaaaat ccttgatgct cctggcgtta atgcttacac acttccagga
    12181 aatgaattct tattaggaaa agctaaagaa gcctttgatg ataatggaaa cattatcaat
    12241 cctgggactg ttcaattttt agaaacttgt cttgataatt ttatcaaata tgtaggagtt
    12301 gtatcatcat tgagaaaacc taaagctatt gaatctgaag acctctactg tcagggacaa
    12361 atcgatacaa ccattactag tatcgatcca gatgatcctg attggttaga caaggcagcc
    12421 gagaaagtta acgccgttga aggggatgct tacgttaaac tagacaatgg tttacttact
    12481 gttaaccaac ttaatatgtt tttaaatgct atgccatttg agctaacata tgcagatgat
    12541 aataatcaat ttctatatta caataataat catccagatc cagatacaat gttaggaaaa
    12601 cgtgttcgtg aacaagtagg aaaccgtatg gccacagttc atggtagttt accaccggca
    12661 cgaatgaaaa atgttgaatg ggttatcggt actcttcgta atggaaatca agaatatgta
    12721 cgtacagtcg ttcctggtac acctccagaa gttatcaata ctcacaacta tcaagctatg
    12781 tactacccag acggatctta tgctggtatt aatgaaatta tctttaactt taaaccgtgg
    12841 ttagattggt acctccaaac tactggacaa cgtttggtcg gtggaactac tgctgctcct
    12901 ggagcttcag cagttgacgc tacttctagc gcttcaacta gcacagagga tgctcccgtt
    12961 gatcaagcca cagatgccac ttctggtgcc tctgaacatt aatctcctcg cttttgagga
    13021 tctgatacct taaaaagata tcaagatcct cctttttaat atttaatcaa gttaataaaa
    13081 tcacataaaa ggaatatact atgtcacttt ttcaagaaaa aattgcttac aattgcgcta
    13141 aaaaggaagc gctttataaa gagagtttag gacgctacgc cttgagatca atgctagcag
    13201 gggcttattt gacaatgagt actgctgccg gtatcgtcgc agctgatact attggtaaaa
    13261 tttctcctgc tctatcaggt tttgtatttg ctttcatctt tagttttgga cttatttatg
    13321 ttttaatatt taatggtgaa ttggcgacat ctaatatgct ttatctcact gcaggagcct
    13381 ataataaaaa tatctcttgg aaaaaagcca taacaatttt aatttattgt acttttttca
    13441 acctcgttgg tgcttgtata ttagcttggt tgtttaatca atcgtactca ttccagcatt
    13501 taacaaatga tagcttttta gggcatgtcg ttgctaaaaa gttaggaaaa cctagttcag
    13561 gagctttcct agaaggtatt attgctaata tgtttgtgaa tttagctatc cttgcttaca
    13621 tgttattaaa agaagaatcg gcaaaaatga cggttatttt atctgctatt tttatgtttg
    13681 tttttctatc aaacgaacac ttaattgcta attttgcatc ctttatgtta gctgctttta
    13741 gccatatcga acatattaaa ggttttactc ttctaaacgt tatccgccaa tggacacttg
    13801 tattttttgg taactggata ggagggggcg ttttcatcgg cctagcctat gcttggttaa
    13861 ataaaacaaa aacttctcac catgaataaa agaaaaactg agtaatttat tactcagttt
    13921 ttcttttata ttctcatcat gaaatcaata actaaaaatt aacttttttg ataaataaag
    13981 gaaagtatca tattaatttt ttaggcaatc cattcaaaat gatatttaaa acaacagctg
    14041 tcagggttga gataacaata ccatttgtca aaaacatttg taaagtatta ggaagactaa
    14101 tgaagagatt tgtctcatta aaacctactc ctgctgcaat tgatacggct gcaatgataa
    14161 agttatgctc attatgctca aaatcaacct gattaagcat tttcatccct tgaagtgcta
    14221 ccataccaaa taaaactaac atagcaccac caagaactgg acttggaatc atttgagcca
    14281 ttgcgccaaa tttaggtaac aagccaagta taactaagaa gagagctgta aaatatatcg
    14341 gcttacgcgt acgtatccca gatatctgta ccagtcccac attttgagaa aaaccagtat
    14401 atggaaaggt attaaataag ccgctaagta atactgccaa tccttctgaa cggtaaccat
    14461 tacgaagtct cttactgtct aatttatcgt ttgtaatatc tgaaagcgca aggtaaacac
    14521 ctgttgattc taccatagaa actgttgcaa taatacacat cattaaaata gaagtaaatt
    14581 caaatctagg agctccaaaa taaaatggct tcggaatatg tacaagtggt gcttctgcta
    14641 ccacagaagc atcaactaag cccataaatg ctgcaagaat tgtacctgaa atgagtccaa
    14701 taaggattga aattgacttc aaaaagcctt ttgcgaaaat attaattaat aaaacaacac
    14761 caatcgttac cagtgataaa gttaaagatt gtaagcttgg ttcttttgca ttatcaccca
    14821 tatttcccat cgcaactggt attaatgtca aaccaattgt agtaataact gatcctgtta
    14881 caattggagg aaagaaattt gcaactttag aaaaaatgcc agcaactaaa acgacatata
    14941 ttcctgaagc aattaaagct ccaaacatat agccagaacc ttgttgtgca ccaataattg
    15001 ataaaggagc tacagactga aaggcacacc ctaatacaac tggaagaccc actccaaagt
    15061 gcttacttaa tcgtaattgt aataaggtgg caatcccaca cataaaaata tctgtcgcaa
    15121 taagataagt tagttgctta gcattataac caagagcact agcaatcatg ataggtacaa
    15181 gaatggatcc tgcatacata gctaacaggt gttgcaagcc tagaagtgct gcttgcgaat
    15241 tactttgctc attaaatttc attaagcatc agcctccgca aaaacaactc gaccattttc
    15301 aaacgcttta atacgagcaa gagaagtaac cggaacacct gttttttcta acaaatcacg
    15361 cccatcttgg aaagattttt caataacgat tccgatacca gcaaccttag ctccagcttg
    15421 accaataatt tcaagtaatc ctttagccgc ttgaccgttt gctaaaaagt catcaatgat
    15481 gagtacagta tcatcgttag ataaaaagcg actcacaata gaaacttgac tcgtaacttg
    15541 ctttgtaaaa gaatacactt cagcagttaa gataccttca gtcatagtaa tgttcttagc
    15601 ttttttagca aatatcattg gtacgcccaa tgcttgagct gcgtacactg ctggcgcaat
    15661 tccagatgct tcaattgtaa caaccttcgt aatgccggct tctttatatt tatcagcaaa
    15721 aactttacct atttcctgca ttaactcaaa atttacctga tgagtcaaaa aagaatcaac
    15781 ttttaaaatg ttctcaccta aaacatcacc atctttaaga atacgttctt ccaataactt
    15841 cataataacc ccttcactct ttagaaaata aaaaaataac ttacctcaat cagaggacac
    15901 aaatctcctc taatttatcg cttcattaac atgaaccaac aggcaagcta tttattagct
    15961 tacttattgt tagacattac ggtgtcttgt agagacgcta agccaatcct cagcataaat
    16021 aagtaaatca tatatttaat tgttatgtat gatagataac tattgtaaca tttttaatta
    16081 agatttcaag atttaattta aatggaatca ccattccaaa tagaattttt aacaataacg
    16141 taatcaacgt gtctcaagct atccaattct tttccacctg cgtaagaaat agaactttgc
    16201 aaatcttgtt gcatttctgt caaagtatcc tctagtcgcc ctttaacagg taatagaatt
    16261 ttcttacctt caacattttt atgttctccc ttttgatatt cagaagctga gccatagtat
    16321 tctttaaatt gctgtccttc tacttccaca agttttccag gactttcaag gtgaccagca
    16381 aacagcgaac caatcattac catactagca ccaaagcgga tagattttgc aatatcccca
    16441 tgtgttcgaa taccaccatc agcaataatt ggcttgcgag cagctttaga acaccaacgt
    16501 aaggcagcta attgccaacc tccagtacca aaacctgtct tgactttagt aatacaaact
    16561 ttaccaggtc caataccaac tttagttgca tctgcacctg cattttccaa ttctctaaca
    16621 gcttcaggcg ttcctacatt tccagcaata acgaaagttc ctggaagctc ttgtttaatg
    16681 tgttggatca tctctataac actgttagaa tggccatgtg caatatcaat agtgataaac
    16741 tcaggagcat cttctttcag acttgtcaca aaatcatatt catagtcttt gaccccaacc
    16801 gaaatcgaag caattaagcc tttatcatgc atacgcttga taaaaggtct tcttgcttct
    16861 tcattaaaac ggtgcataat ataaaaataa ccttcacacg ctaatgtttc agctacctct
    16921 tcatcaataa tagtctgcat attagcaggg attactggaa gtttaaaggt atagtttcca
    16981 agtttgacac ttgtgtcagc ttgtgagcgg ctagatataa tacacttgtt tggtattaat
    17041 tgaatatctt cataatcaaa aacaggaata tcgttaaaca taatggttct cctaatgagt
    17101 aaaatgacct tcctataact ttgtataaca aagcatcgct aggtaaaaac atacctttcc
    17161 tttttaaatt atatacttta tttattttta gtcaattttt gatcaattta tttgaataat
    17221 gttcgttttt ttcaaaataa atgtaaaatc ctatagagca gtactctata ggattttaca
    17281 tttattatat tgcttttctt tttcgaagat aagttaatcc tacaatagct aagataacaa
    17341 caacgataac gaatagaact gcaaattgac taccaattat agctccattt ttcggaatag
    17401 ctacttgtcc gatggtaaaa atttgtgagg ctactgcggt tgcaaaagct cccgcaaatt
    17461 gttgtagtgt atttaagata ctattaccat ccgctgactg tgctggcttt agttttgtta
    17521 aagctgtcgt aaccatatta cttgctccaa tccccatacc aattttagta agaatatcaa
    17581 aggctaaaag tactacaaca aacttcgcag gaatgaatat agctgtacca attaaaccta
    17641 tcaaagaaag ggagatacct atcataattg gcttaaatgc accaatttta tccaggagat
    17701 aacctgcaaa aggggataaa aaaactactg ctattgcacc tggaaacata aacaaaccag
    17761 ctttggttga agtttgatgt aaaacaagtt gcaagaggtt aggtaataag aacgaaagtg
    17821 ctagaggaat catttgaaaa gccaggacgc catacaataa gattgagaaa tctttatctt
    17881 taaaaacatt caaattcaaa aattctagct gtttatttaa atagtaaaaa ataacaaaac
    17941 taacaatagc tactaataag taaaaaatag acatcttttc aatagctaaa agaagggttg
    18001 ccaagccaat acctaaactg acaaaggcag gaaaattgag tttatcatta attcttactt
    18061 gacgtttggg aatagaagcc aaacctataa aggtagatat gataagtatt ggtgccagaa
    18121 gcataaaaat catcttccat cctaacattc ctgaaatgac accaccataa gtaggtccaa
    18181 ctgccggtgc taaaagtgtg accatagcag ctactcccat cattagtcct ctacgctcca
    18241 tcggaatttg tgtaagaatg atatgaaaca ataaagggag ggctaatcct gtcccaacac
    18301 cttgcaagac tctccctact aacaatatag ctaaattagg ggcaaagcta tcaattaaaa
    18361 caccaaataa aaaaagcaca tttgccaatg taaaaatttg tctctctgat aaatttttaa
    18421 tcataaatgc tgacaaaggc acagtcacag caacagctaa caaatttcct gttgtcaccc
    18481 attgaataac agcggggtta accccaaatt ctttcatcaa aagcggaaaa gtgacattca
    18541 tagatgtttc aataagaatc cctgaaaaac ctaatatagc cgttgtaatt atggcaggca
    18601 taatacgcca tcccttttta gtcattaaaa tctccttact ttgattatta tcataaaata
    18661 gagcaccccg ctaaatcaag gtgctccatt taattcattc gaattaaaat taaattgttt
    18721 atattagttg tcaaaggaca atcaacagct cttattatat caaataaagc taagaaaaac
    18781 aaagagtttt ttgaatatta atgccttcac tttcttatct attctctacc catcaatcac
    18841 cacttcagag aacataacaa ttgcaattta ctcttaatac cgttatcatg ttcatatgct
    18901 aatacccaag aaaatagtgg cttttgcctc aaatgaatct gaaaataatt tctttattac
    18961 gttaaagact tactttaatt atttgttttc tattcaaatc attacagata tttcaactct
    19021 caatcatgca gattttgacg gtagctttgc attccatgat atagaaacta gcatacctca
    19081 tctggtaatt gactccaact acttagcgat tagtcagact aactcaaaaa cagaggctaa
    19141 cgatattaaa acgttctcgg aactctccaa aacaatgacc gagttttact atatgctaaa
    19201 ttttgactta tttaaccatc tcccatatcg ttttagattg cataataagg acggtcaaac
    19261 aatttattcg aaccataaac ctgaagatcc ttttgatatt tatcctgaag aagaatatcc
    19321 tatagacaaa tgggttcaaa acagtctaat agagaaaaaa gcgaaagagc tacatttact
    19381 cttacctggc gcatcacaag attatattct agtacaatct tataaacgct tagaaaatga
    19441 tagtgggcaa ctagttggtt atattgaaca tgtccataat attaaaccac tattagaggg
    19501 gtatttaaaa gaatccggtc aagctatcgt tggatggtct gatgtcacgt ctggtgcttc
    19561 tatctctaac gatgattttg aactataatc cactcgtcac tctaaaaaga aaactaataa
    19621 ctaaaaggca ctggtttaat tccagcgcct tttattaatc aatctccagt tgttgtaagt
    19681 tcaatttttt ctttaattgg tagacctcga ttagctgtaa aaatcaaagg aactacttct
    19741 gttgtaactt cggaaaattg tagaccaaac catcgtattg gtgttgctgt ccaatgcttt
    19801 acagatgatt tcattagcat aacaaatcgt tcaccaggtt ttaggttttg actgcttgaa
    19861 aaacgttcat caaggacaat aaacctaaaa tctcctactt tactatcagg tctaacgctg
    19921 tatgtctttc cttgtttagg cagacgtcca ctttcaagta attcttctac aatcgtacga
    19981 agatagcgtg acacagtctg acgcatctta aatccaaggt aaagttctac cttcacaatg
    20041 aagtcggttc ccatcatgtc aactttatac tcagctgtat aaggttcatc agtgacctta
    20101 atgtttacaa accaataaac ttgtgctttt ttagggcgct tatcaaggat agaatacatg
    20161 atagacttat caatcatgtc tccttccata cgatttgtca aataaacaac gttagtatga
    20221 taaatcggat aacgatgatc atgtctaagt ttatctaatt gtccgatgta atcttttaaa
    20281 tccaacaact taacataacg cgaaacaatt ttactacctt tataccagat agtcatgatg
    20341 aatataattg ctactgctat aataacaaca acatagcctc catgcataaa tttaacagct
    20401 gaagcaagga agaagattcc ctctaaaata ccaaagaaaa tcatcatcaa taagactaaa
    20461 cctcttttta ctcctttttg aattaaaaag aaagacagta aaatagttgt cattagcatc
    20521 gtaattgtta tcgctaatcc atatgctgct tccatgtgcg ctgaagtttt aaaaagcaaa
    20581 acaatagagg ttgtaatggc aaataagaac caattaataa caggtatgta ggtttgacca
    20641 atattgtcac caggataagt tgaacgaaat tgtgggaata tttttagtcg catagcttca
    20701 gaaactaagg taaatgatcc agaaatcagt gcctgtgaag cgatgatagc tgccaaagta
    20761 gctaaaataa cgacatgcat tgtaaattgc gaaggaatac tagcaaaaaa gggattcaat
    20821 tcatttcctg cgttcttatt agctaaaatc catgcccctt gcccacaata agaaagtata
    20881 atggcaacct taacaaacgg ccatgaaaca tgtatatttc cacgcccaac atgacctaag
    20941 tcagagtata gtgcttctgc tcccgttgtc gccaggaaaa tagatcctaa aataaaaata
    21001 cctttatgat tctctggact aaataacaat ttcaaaccgt agtatggatt aattgctttg
    21061 aaaacttctg gatgggcaaa actattaagg agaccgctaa tacctaaaaa ggcaaaccat
    21121 ataaacataa ttggcccaaa taatttccca ataacacccg ttccaaacct ttggatggca
    21181 aaaagtaaca gtaaaataaa caaggtcgca aaaataacat tactctgatt ctggaaaata
    21241 tgctgcaaac taggaactac ttttaatcct tcaacggctg aagttacggt tacagctggc
    21301 gtcaaagctc catctgacaa caaggttgca cctccaataa cagccggaac aattaaccaa
    21361 ggtgtcattt ttctaacaag ggtatataaa gaaaaaatac caccttcgtg gtgattatcc
    21421 gcctttaaag ctacaagcac atacttgata gttgtaataa gtgtcaaggt ccatatgatt
    21481 aaagatatag aacctaagat aaacgattct gtgacactag aaataccacc ttggttttca
    21541 accaatgatt gcatcgtata gagtgggctt gtaccaatat ctccataaac aatgcctaaa
    21601 gcaataataa atcctgcttt tgatgcttta tcaaaagaag aatgattgac atgttgcatt
    21661 tctgccaacc ctccgattaa ataatattag tcaaaaaaat ggctgaactc attatagcag
    21721 attaagttaa aaaaacaaca cttatctata aaaagaccag aaattattaa agcgtttcct
    21781 ggtctttact attttaatca cgataaacag gttgaggtcc ataagtttga ctgactggca
    21841 ttatttctat cgattaatat taatgtgttc tggttggcta gcaacccatg acacggtatt
    21901 agcaatatcc tctgcttgaa ttgcatgcgc accttcataa agtttttcaa ctcttttatg
    21961 atctcctttg aaacgtacag ttgaaaattc tgtcccttca cataaaccgg gttctaggtt
    22021 agtaactctg attttagttc cagctaaatc ggcacgcaag ttgagagcaa attgttttac
    22081 aaaagctttt gatgcgccat agacattagc tcctggataa ggaattgttc ctgcagttga
    22141 tcccaaattg ataattaacc ctctattaac ttctaccatt ttcggtaaaa tgcatcgtgt
    22201 caagtaaatt aatcctacaa cgttagtatt aatcatagtc atccaatctt caaaatcagc
    22261 ttcatatgat ttatccaatc caagtgctaa acctgcatta ttaaccaaca aatcaattga
    22321 ttgcaaattt ttaggcagac tttctaaagc tttgtcaacc gattctctac ttgtaatatc
    22381 catttaaagt ggataaaagt aaccctctga aaacttttct ccaaggcatt ttaatttatc
    22441 cattcgacga gcacaaccaa taacgctata accatctgaa actaatttag taacaacagc
    22501 tgcaccaaaa ccagcagatg cccccgtcac aagcgcaact ttacgtgata tcataaatac
    22561 tcctcctcta gtatatagaa aaacgagcta ggaaaaatcc ttctcgtttt ataacttatt
    22621 ttgatttctc atcaatagct tgagcagctg taatgatagc aagtttgtaa atatcttcag
    22681 cacttgatcc acgtgataaa tcatttactg gagcattcaa tccttgtaag atcggtccga
    22741 ttgcttcaaa catacccaaa cgttgtgcaa ttttatagcc aatatttcct gattgaaggt
    22801 ctgggaaaat aaaaacattt gcttttccag caacatcact attaggagct ttaatttctg
    22861 cagtttccgg aacaaaagct gcatcaaatt gtaattctcc atcaacagct aattcgggag
    22921 ataaatcttt ggcaatttta gctgcttctt gaactttttc agcttgaggc gcttttgcac
    22981 ttccttttgt tgaaaaacta agcatagcaa tcttgggatt aatatcaaat attttggctg
    23041 tatccgcagt atttactgca atttctgcta gctcttgtgc attaggatca atgttaatag
    23101 cacagtcagc aaaaatataa cgttcttgtg tattttcacg gttcatcaag aatacacctg
    23161 aagtgcgtga aattcctggt tttgttttaa taatttgaag agcaggacgt acagtatcag
    23221 ctgttgaatg aatggcccct gataccatac catctgccaa accaagtttt actaacataa
    23281 ccccaaaata gttaacatcc ttcaaaagac gatctgcatc ttccatagta gctttgcctt
    23341 tacgaatctc aacaaaagct tctttcatct cttcaaattt ttcgtattgt tcagggtcaa
    23401 taacgctata atcttgatca acaaaaccaa gtcttgccaa agaatcgtga acttcgtcag
    23461 ccttacctaa aataacagga tccacaagac cttcaaattt caaacgagct gctgcacgta
    23521 atacacgttc atcgcttccc tcagggaaaa caatcttaac ataatcactt gcaacttttt
    23581 ctcttaaacc accaaataag gaacgcatat atataaaacc ttctatttct aaattaatgt
    23641 aattccatga taacgctgtc aaagtcatct gtcaagtcta gcgtctgttt atttattttt
    23701 cccataaaag ggtcgtaaaa agacaaagaa tgacaatgca atgcttgcct gtttattcct
    23761 agatccatac gtcctccgta caaatcatct cccaaaagag gaaagccgat atgagcaaag
    23821 tgaactctaa tttgatgagt acgccctgta tgtaatttga tatccactaa tctaacatca
    23881 ccataacgag caactacttg ataactagta tgtgcatatt tgcctgattc atggactcgc
    23941 ctcgtaataa tactatcaac atctcgtgca ataggagcta taatatcacc actatctgct
    24001 aaatcaccac tccctgatac taatgcataa tacctttttt caattgcttt agcctgaagt
    24061 tgtttatcta gacgagcatg ggcataacca tgcttggcga ataacattaa tccacttgta
    24121 tctcgatcca accgggtcac aatatggacc tgctgattgg catagttatt agacacataa
    24181 tagtgtttta taaaattagc aattgtattt gaatgaatag agctaggaat actagcgaaa
    24241 ccaaaaggtt tatttaaaat caaaaaatga tcatcctcat aaacaatatc taaatcgtga
    24301 ggtactggtt ccaaagtttc gtgagaaggc tcgtcaggaa tatctatcgt aacaacatcg
    24361 ccaatctcta ataagaaaat agcgttctgc tcttcaccat tgacaaaaat cttaccacct
    24421 ctgtacttaa tctttgctaa taaccccctt gaaacatcat gacttttcaa aagtgtttta
    24481 actttgcacc tttcttctgc tatgtattca aatttcaatg ttcaggttct ccgataaaag
    24541 catcgttaac acgttcccaa aagctagtgt gacttggagt tgaaacaaag ttaatacttt
    24601 tttcgtcaat actatattct attttggtaa catttttgta atggaccgtt ttgttatcta
    24661 tagaaattgt atagacacca actcgtttag gaactatctc tatagcatct tttttgggga
    24721 tgataaccga tgaccccaag gtacgataga cgcgattatt aagactagaa atttctgtta
    24781 gttgcaaggc ttctatcgtt gggtggagta ctgccccccc tagtgattta ttgtaagcag
    24841 tactaccggt tggtgtagaa actaaaatgc catctcccct aaacctttca aataccactt
    24901 gattaataac aacatccgct accattgttt tttcaatacg cttaatagta gactcattta
    24961 aagcacgcgc acgaataact cgaccatctt caagggtaat agttactttc aaaataggat
    25021 aggagatttg ttcaccttta tcatttttca aattattaat taatgtgtca acttcaaaat
    25081 ctcgatagtc tgtataaaac cctaaatgtc ctgtatgaac accgacaaac cttactttat
    25141 ccagttgttt ttcgtacata tggaaggctg acaaaagcat accatctcca ccaatagaaa
    25201 tgacaatatc aggatccttt tttgataaat aaaaatcagg atcatgttta aaagcagcaa
    25261 agagctttga ggctacacgc ttgctttgat attttccgtt agcaattata gcaacacgtg
    25321 ttgctctatc tgtaaaattc atctgtgtca tcactatttc ctacaccatc gctcaatttt
    25381 cgatttaaag gatcaaataa aagctgtgct tcacgaatat cttcgcgaat cttacgcatt
    25441 tcttcatcca attctaaagc gatttttgct gttttctcca gtctttgttt aatttcttca
    25501 gggaagtccc cctgatactt gtaatttaat gaatgttcga tggttgccca aaaattcata
    25561 gccagagttc taatttgtat ttctgctaaa actttctttt gtccatctat tgtgtctaca
    25621 ggatactcta caacgacatg atatgaacga tatccactag atttcatatg ggtaatatag
    25681 tctcgctcct gaaccactgt catgtcatga cgttttctca acaaggcaag gacttcatca
    25741 acgtcatcta caaattgtac catgatacga agacctgcaa tatcttgcaa gtcctgagca
    25801 agattttctt ctgaaatacc acgtaaaacc atcttttctt gaatactttc aaccgacttt
    25861 acgcgtccag taacaaattc tattggagag tgacgatttt gctttctaaa ttgtttacga
    25921 ataccacgaa gctttatttt taattcacca accgtttgaa tataagggtc tagaaatgtt
    25981 tcccaatcca ttgacattct aatcctccaa aatatttaat gtactctctt gtaaaataaa
    26041 ctttttttct ataaaattaa cacaacaatt aatattatac catatttagg aaaaggaagt
    26101 gataagatga cacacctaga aattgaatac aaaactctat tgaataaaga cgaatttaat
    26161 cgtctaacaa gcttattttc tcatgttcaa cctattactc aaaccaatta ctattttgac
    26221 acagaaacat ttgaaatgaa agcacaccga atgtcacttc gcattagaac gttacctaac
    26281 cgtgccgaac taacactaaa aatccctaga gaagtaggaa atttagagca caatcatgac
    26341 cttactttag aagaagctaa atatatcgtt aaaaatggcc agttccctga agatactgag
    26401 attgcttctt taatccttga aaagggagtt gatccaacca aattagctgt ctttggacaa
    26461 cttacaacaa caagacgaga gatggaaact tcaatcggct taatggctct agacagcaat
    26521 atatatgctg atattaaaga ttatgaactt gagttagaag tcaaacaacc taaacaaggg
    26581 aaaagagatt tcgaccagtt tttaaaagaa aataatatta attttaaata cgccaaaagt
    26641 aaggtggcta gattctctgc tactttaaaa aataactaac gttgagagat tggattttcc
    26701 aatctctttt ataattctca tttaagcaag aacttaaaat ctcatactat ctctcctcaa
    26761 cattgttatt gcaagcattt tcatgtttct tatcgtgaat tttttctatt tttttggtaa
    26821 actattagta tcaatctaaa ggagtcatct atctcatggc agaacaatac gccgataaac
    26881 aaatcaaact tttctcatta accgctaata gagaaattgc tgaaaaaatt tcccaagctt
    26941 caggaattcc tttgggtaag atgtcatcac gtcaattctc tgatggcgaa attatgatta
    27001 atattgagga aacggtgcgc ggtgacgata tctatatcat ccaatctaca agcttcccag
    27061 tcaatgataa tctctgggaa ttactcatta tgattgacgc ttgcaaacgt gcaagtgcta
    27121 atactgttaa catcgtagtc ccttattttg gatactctcg tcaggaccgc atcgctgcat
    27181 ctcgtgaacc tattactgca aaattggtcg ctaatatgtt agttaaagct ggagttgatc
    27241 gagttcttac cctcgactta catgcagtgc aagtacaagg gttcttcgat atcccagttg
    27301 acaatctttt tactgtccca ctatttgctg aacactataa tcaactcggt ctctctggtg
    27361 aagatgtcgt cgttgttagt cctaaaaatt ctggaatcaa gcgtgctcgt agtttggcag
    27421 aataccttga ctctccaatt gctatcattg attatgcaca agatgattct gaacgtgaag
    27481 aaggctatat tattggtgag gtagaaggta agaaagctat tattattgat gatattttaa
    27541 atacaggaaa aacctttgcc gaagctgcaa aaattttaga acgtggtgga gcaactgaga
    27601 tttatgctgt tgctagtcat gggctttttg ctggcggcgc agctgatatt ttagagtctg
    27661 caccaattag agaaattatc gtaacagact ctgttttatc taaagagcgt attcctagca
    27721 atattaaata tctgaccgct agccatctca ttgcagacgc tattatccgt atccatgaaa
    27781 gaaaaccttt aagtccatta ttttcatatc gttcagataa gaaggattaa ttgatgattt
    27841 atttagacaa tgctgctact accgctctaa ccccatctgt tattgagaaa atgaccaatg
    27901 tcatgacaag taactatggt aatccatcta gtatacatac ctttgggcgt caagcaaatc
    27961 aacttttacg tgaatgtcga caaattattg ctgaatatct aaatgttaat tcacgtgaaa
    28021 ttattttcac ttctggggga actgagagca acaatacagc tatcaaaggg tatgctcttg
    28081 caaatcagct aaaaggtaaa catattatta cctctgaaat tgaacatcat tcagtcctac
    28141 atactatgac ttacttatca gagcgatttg gttttgatat tacttactta aaaccaaacc
    28201 atggacaaat tactgcaaaa gacgttcaag aagctttacg agatgatact attatggtat
    28261 ctctcatgtt tgctaataat gaaaccgggg actttttacc aattcaagag attggtcagc
    28321 ttctcaggaa ccaccaagct gtttttcacg ttgatgccgt tcaagtcttt agcaaaatgg
    28381 aacttgatcc tcattcttta ggaattgact ttttagctgc ttctgcccat aaatttcacg
    28441 gtccaaaagg tgttgggata ctttactgtg ctccccatca ctttgatagt ctacttcatg
    28501 gtggagacca agaggaaaaa aggcgtgctt caactgaaaa tataattggt attgctggaa
    28561 tgtctcaagc tcttactgat gctacgacta acacccttaa aaattggact cacattagtc
    28621 agctgagaac gaccttttta gatgctattt cagaccttga cttctatctt aataacggtc
    28681 aagactgctt acctcatgta cttaatatag gttttcctag acagaataat ggcttgttat
    28741 tgacacagtt agatttagct ggattcgcag tttcaacagg ttctgcatgt actgcaggaa
    28801 cagtcgaacc tagtcatgtc ttaacaagct tgtatggagc caactcacca cgtctaaatg
    28861 aatcaatacg tattagtttt tcagaactaa atacccaaga agaaattctt gaattagcta
    28921 aaaccttaag aaaaattata ggagattaat atggcatttg aaaaagaaat aaatcttcca
    28981 gactgcaatt acatttacac tatcagcccg aacattaaaa aatatacctt acgcgatact
    29041 acattcactc aaaatcgctt aggtaattat gaattagagc gtctcctaga aacagtacct
    29101 aattctggcg atggctttcc tctaaagatt acaatcaata aagaactttc tggttttaag
    29161 ctcttaatta ctgataaatc tggccttaga atagtaaata tctttaaatc taccgatcat
    29221 aaaattcttc aagacaaatt ttatttctta atggacagcc tcgttgagcg taatatcttt
    29281 acgaaaactg caaaataatt taccgaccac tatttttaac attttagtgg tctttttgcg
    29341 a
//
LOCUS       DATROD010000013        28728 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.013, whole genome shotgun sequence.
ACCESSION   DATROD010000013 DATROD010000000
VERSION     DATROD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28728
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.013"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            4..2265
                     /locus_tag="VBC74_000596"
     CDS             4..2265
                     /locus_tag="VBC74_000596"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEO6941568.1"
                     /translation="MPTKPNEAFEELKGLGFPLNKKNSDKANLEAFLRHSFLNQTDTD
                     YALSLLIVDAKTDALTFFKSNSDLTLENLQWIYLQLLGFIPFVDFKDPKAFLQDINFP
                     VSYDNIFQSLHHLLACRGKSGNTLIDQLVADGLLHADNHYHFFNGKSLATFNTNQLIR
                     EVVYVETSLDTMSSGEHDLVKVNIIRPTTEHTIPTMMTASPYHQGINDPAADQKTYQM
                     EGALAVKQPKHIQVDTKPFKEEVKHPSKLPISPATESFTHIDSYSLNDYFLSRGFANI
                     YVSGVGTAGSTGFMTSGDYQQIQSFKAVIDWLNGKVTAFTSHKRDKQVKANWSNGLVA
                     TTGKSYLGTMSTGLATTGVEGLKVIIAEAAISTWYDYYRENGLVCSPGGYPGEDLDVL
                     TELTYSRNLLAGDYIKNNDCYQALLNEQSKAIDRQSGDYNQYWHDRNYLTHVNNVKSR
                     VVYTHGLQDWNVKPRHVYKVFNALPQTIKKHLFLHQGQHVYMHNWQSIDFRESMNALL
                     SQELLGIDNHFQLEEVIWQDNTTEQTWQVLDAFGGNHQEQIGLGDSKKLIDNHYDKEA
                     FDTYCKDFNVFKNDLFKGNNKTNQITINLPLKKNYLLNGQCKLHLRVKTSDKKAILSA
                     QILDYGPKKRFKDTPTITFLNSLDNGKNFAREALRELPFTKDHYRVISKGVLNLQNRT
                     DLLTIEAIEPEQWFDIEFSLQPSIYQLSKGDNLRIILYTTDFEHTIRDNASYSITVDL
                     SQSYLTIPTNQGN"
     gene            2269..2628
                     /locus_tag="VBC74_000597"
     CDS             2269..2628
                     /locus_tag="VBC74_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941569.1"
                     /translation="MKLLTKERFDDSQHFWYQINLLQESNFGAVFDHDNKNIPQVVAT
                     IVDDLQGSGSSNYFWYFGNTTDTSILMIAHLNRKFYIQVNLKNFDFALNLIAINNWKS
                     LLQTQLEALNDTLAIFQ"
     gene            2674..3654
                     /locus_tag="VBC74_000598"
     CDS             2674..3654
                     /locus_tag="VBC74_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO6941570.1"
                     /translation="MSSYWNNYPELKKNIDETNQLIQERIQVRNKDIEAALSQLTAAG
                     GKQLRPAFFYLFSQLGNKENQDTQQLKKIAASLEILHVATLIHDDVIDDSPLRRGNMT
                     IQSKFGKDIAVYTGDLLFTVFFDLILESMADTPFMRINAKSMRKILMGELDQMHLRYN
                     QQQGIHHYLRAISGKTAELFKLASKEGAYFGGAEKEVVRLAGHIGFNIGMTFQILDDI
                     LDYTADKKTFNKPVLEDLAQGIYSLPLLLAIEENPDIFKPILDKKTDMATEDMEKIAY
                     LVVSHRGVDKARHLARKFTEKAISDINKLPQSSAKKQLLQLTNYLLKRKI"
     gene            complement(3739..5487)
                     /gene="cydC"
                     /locus_tag="VBC74_000599"
     CDS             complement(3739..5487)
                     /gene="cydC"
                     /locus_tag="VBC74_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEO6941571.1"
                     /translation="MFKIPLFKELKTDQWIKPFFKQYKVSLVIALFLGFMTFFSASAL
                     MFNSGYLISKSASLPSNILLVYVPIVLTRAFGIGRPVFRYIERLTSHNWVLRMTSQLR
                     LKLYHSLESNAIFMKRDFRLGDVMGLLAEDINYLQNLYLRTIFPTIIAWILYSFIIIA
                     TGFFSLWFALMMLLYLAIMIFLFPLWSILANGARQTREKELKNHLYTDLTDNVLGISD
                     WIFSQRGQEYVTLHERSESELMAVQKKIRSFDNRRALIVELVFGFLAILVIIWASNQF
                     IGHRGGEANWIAAFVLTVFPLSEAFAGLSAAAQETNKYSDSIHRLNELSETYFETTQN
                     QLPNKPYDFSVKNLSFQYKPQEKWVLHHLDLDIKEGEKIAILGRSGSGKSTLASLLRG
                     DLKASQGEITLGGADVSIVGDCISNYIGVIQQAPYLFNTTLLNNIRIGNQDASEEDVW
                     KVLERVGLKEMVTDLSDGLYTMVDEAGLRFSGGERHRIALARILLKDVPIVILDEPTV
                     GLDPITEQALLRVFMKELEGKTLVWITHHLKGIEHADRILFIENGQLELEGSPQELSQ
                     SSQRYRQLKAADDGDL"
     gene            complement(5480..7198)
                     /gene="cydD"
                     /locus_tag="VBC74_000600"
     CDS             complement(5480..7198)
                     /gene="cydD"
                     /locus_tag="VBC74_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HEO6941572.1"
                     /translation="MLDKAVMRLSGIHKLLGLLAGLDVLQAIFIIGQAYYLSLSITGL
                     WEGQKLSSQTVYVLLFMVSYLGRHVIDYIKNRKLDDFSTAQSSLLRRQLLDKLFDLGP
                     KVVQEQGTGNVVTMALDGVSLVENYLRLVLNKMINMSIIPWIILAYIFYLDIESGAIL
                     LIVFPLIIIFMIILGYAAQAKADKQYESYQVLSNHFLDSLRGIDTLKYFGLSKRYGKS
                     IYQTSESFRKATMSTLKIGILSTFALDFFTTLSIAIVAVFLGLRLLNEQIYLLPALTI
                     LILSPEYFLPVRDFSSDYHATLDGKNAFQAIQKVLNKTGIKGEQLVIDDWSKESRLDL
                     ENIAIAYDQKRVVEDVTLRFRGHQKVALVGVSGSGKSSLINLLSGFLGPDNGSLKVDG
                     REVTNLDQEDWHKQMIYIPQTPYVFEMSLRDNITFYTPNASDEEVVRAIHMVGLDSLL
                     SELPDGLETRIGNGARPLSGGQAQRIALARAFLDQNRRIMVFDEPTAHLDIETELELK
                     EKMLPLMSDRLVIFATHRLHWLNQMDVIVVMEKGRVAEVGSYQELLAKKGYLYQLKHA
                     MGGNDV"
     gene            complement(7198..8217)
                     /gene="cydB"
                     /locus_tag="VBC74_000601"
     CDS             complement(7198..8217)
                     /gene="cydB"
                     /locus_tag="VBC74_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEO6941573.1"
                     /translation="MSALQFFWFFLIGLLFSGFFFLEGFDFGVGMAVQTLTHNEHEKD
                     QVVETIGPVWDGNEVWLLTGGGAMFASFPYWYASLFSGYYLILLTILFGLIIRGVSFE
                     FRHKVPAEKKQFWNWTLTIGSAIVPFFFGIMFISLIQGMPLDASGNLSAQFSDYFNIF
                     SLVGGVAMVLLAYLHGLNYIALKTEGPIRERARNYAQLLYWVLYLGLALFAVLLYFKT
                     DFFSNHPIVTTIMVLVIVVLAVLAHASTFKGAEMTAFLASGLSLVSVVVLLFQGLFPR
                     VMISSISPKYDLLIQNASSTPYTLKVMSIVAITLVPFVLAYTAWAYYIFRKRITLPVI
                     VTGEK"
     gene            complement(8218..9645)
                     /locus_tag="VBC74_000602"
     CDS             complement(8218..9645)
                     /locus_tag="VBC74_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEO6941574.1"
                     /translation="MTIETLARFQFAMTTVFHFFFVPFTIGTCLVVAIMETMYVITKN
                     EEYKKLTKFWGNIMLLSFAVGVVTGIIQEFQFGMNWSDYSRFVGDIFGAPLAIEALLA
                     FFMESTFLGLWMFTWDNKKISKKLHVTFIWLVVFGSLMSAMWILTANSFMQHPVGYEV
                     VNGRAQMTDFLALVKNPQFFYEFTHVIFGAITMGGTVVAGMSAFRLLKSEQLKDTTVE
                     LYKKSVRIGLVVALLGSISVMGVGDLQMKALIHDQPMKFAAMEGDYEDSGDPAAWSVV
                     AWANEAEHKQVFGIKIPYMLSILSYGKPSGSVKGMDTANKELVAKYGKDNYYPMVNLL
                     FYGFRTMAAMGTAIMGVSVLGLFLTRKKKPILYKHKWMLWIVALTTFAPFLANTFGWI
                     VTEQGRYPWTVYGLFKIKDSVSPNVSVASLFVSNTVYFLLFGGLAVMMISLTIRELKK
                     GPEYEDEHGHHGAYTSIDPFEKGAY"
     gene            complement(9748..10956)
                     /locus_tag="VBC74_000603"
     CDS             complement(9748..10956)
                     /locus_tag="VBC74_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6941575.1"
                     /translation="MKEILVLGAGYAGLKAVRNLQKQSGDFHITLVDMNDYHYEATEL
                     HEVAAGSQPKEKITFPIKDVINTNKVNFMQDEVLRVDAENKTVTVKNNGELHYDYVVV
                     ALGFVSETFGIKGAMENALQMTNISQAENIHNHIVNTMKLYRETKDENLLKLLVCGAG
                     FTGIELAGAMVDERPKYAALAGVKPEQIEIICVEAATRILPMFDDELAQYGVNLIKDL
                     GINLMLGSMIKEIKPGEVVYGTSKEDEELKSITAGTIIWTTGVSGSPVMGESGFDQRR
                     GRVMVNSDLRDPKYDNVYVIGDVSAFMDTESGRPFPTTAQIATRMGAHVAKNLLHQIK
                     GEATEDFSYSPQGTVASVGNTHGLGVVGKTKIKKYPASVMKKIIMNKSLVDMGGLKEL
                     LAKGRFDLYH"
     gene            complement(10969..11868)
                     /locus_tag="VBC74_000604"
     CDS             complement(10969..11868)
                     /locus_tag="VBC74_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEO6941576.1"
                     /translation="MTLPIFLELVEMKAKTASVLPFLIGLCFSAYYYNSVHPVYVGLF
                     FVAMFLFNMFVDIWNNYNDYRNAVDLDYKNDTNIIGRENLSLRQIEVIMASLVITSSM
                     IGLVLVSQVGLPLLWMGLFCFGIGVLYSFGPRPLSSLPLGEVFSGLTMGFMISLICVY
                     LNTYQNFSWDILNLSKIFLISLPNTLWIANLMLANNLCDKEEDEKNHRYTLVHYTGIR
                     GGLLLFAISNSIALLAIVFEFLFGLAPVTVLLSLLLIPFIYKQTKLLWQKQVKRETFV
                     CAVRILALGSATQVLTYLIGIIF"
     gene            complement(12277..12882)
                     /locus_tag="VBC74_000605"
     CDS             complement(12277..12882)
                     /locus_tag="VBC74_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941577.1"
                     /translation="MNKLITEKKVNNVSTANYLKLGLVSAMFAGGAFVALGSTQGVSA
                     STFTAPQATPPKAERQLTDSELYERAQKQVLPRYIQGSLSGILNQHSTLYKQQNAAVT
                     PQVSSPKSERQLTDSELYESAQKQVLPKYIQGSLSGILNQHSTLYKQQNAAVTPQVSS
                     PKSERQLTDSEIYERAQKQVLPNYIQGSLSGILNQHSTLNA"
     gene            13765..14064
                     /locus_tag="VBC74_000606"
     CDS             13765..14064
                     /locus_tag="VBC74_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEO6941578.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQTQADLAASQFTGKSAQELVTVTFT
                     GDKKLISIDYKEAVVDPEDIETLQDMTTQAINDALSQVDDATKKIMGAFAGKMPF"
     gene            14224..15414
                     /locus_tag="VBC74_000607"
     CDS             14224..15414
                     /locus_tag="VBC74_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6941579.1"
                     /translation="MVMDSLIIKQLIKSTFDIPLQVTYPNGNIETYNGSNPHVKLKLN
                     KNFSVSELSKDPSIVLGEAVMDGDIEIYGSIQELILSAYRCGDSFLRNSKFSKLIPKQ
                     FHDKKHSKSDIQKHYDIGNDFYKLWLDDTMTYSCAYFKHENDSLEQAQLNKVHHILNK
                     LNAQPGGKLLDIGCGWGTLIITAAKEYGLNATGITLSEEQASFITKRIKEEGLENKVT
                     VLIKDYRDIRETYDYITSVGMFEHVGKENLSQYFQTISKRLNINGLALIHGITGQVGG
                     NHGSGTNSWINKYIFPGGYIPRLTENLNHIASAGLQIADLEPLRRHYQKTLELWTKNF
                     HNALPEVQKTHDKRFINMWDLYLQSCAASFESGNIDIFQYLLSKGVSKDTMPMTRDYM
                     YSAN"
     gene            complement(15484..16209)
                     /locus_tag="VBC74_000608"
                     /pseudo
     CDS             complement(15484..16209)
                     /locus_tag="VBC74_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(16336..16923)
                     /locus_tag="VBC74_000609"
     CDS             complement(16336..16923)
                     /locus_tag="VBC74_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEO6941580.1"
                     /translation="MKQLQEYIAFDLEFNTVGEHSHIIQVSAVKYSNHQEIALFDTYV
                     HTKVPLQSFINGLTGITARDIIGAPKIEIVLTDFQSFVGDTPLIGYNGYKSDLPLLVE
                     NGLDLTSQYQVDLYDEAFVRRSTDLNGIVNLKLTTVADFLGIKGKAHNSLEDARMTAR
                     VYEKFLDLDENKIYLKQQKEVAVDSPFATLGNLFD"
     gene            complement(16967..17503)
                     /locus_tag="VBC74_000610"
     CDS             complement(16967..17503)
                     /locus_tag="VBC74_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEO6941581.1"
                     /translation="MTVEKTVSELAEILGVSRQAMNNRVKTLPEECVEKNSKGVTVVN
                     RDGLIKLEEIYKKTILEEEPIDEEASRRELLEILVDEKNTEITRLYEQLKAKDIQIAS
                     KDEQLHVKDIQIAEKDKQLDQQQQLTLTAMEDTQRLQLELNEAKAEVEEIQEAKEEKI
                     QELEAVKKSFFGRFFNKK"
     gene            17638..18810
                     /locus_tag="VBC74_000611"
     CDS             17638..18810
                     /locus_tag="VBC74_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6941582.1"
                     /translation="MKHYDTIIIGGGPAGMMAAISSNFYGNKTLLIEKNKRLGKKLAG
                     TGGGRCNVTNNGNLDELLAGIPGNGRFLYSVFSQFDNHDIINFFQDNGVTLKEEDHGR
                     MFPTTDKSRTIINALENKIKELGGQIMTDTEVVSVKKIGDSFYIKTKDTQFASDKLIV
                     TTGGKSYPSTGSTGFGHDIARHFKLEVTDMEAAESPLLTDFPHKKLQGISLDDVTLSF
                     EKHIITHDLLFTHFGLSGPAALRISSFVKGGETIYLDVLPNISVKELEIHFQNEREKS
                     LKNALKILLPERLAEFYAEDLPEKVKQVSVKDLEMLIQKLKKLPILVTGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVAGLHFAGEVLDINAHTGGFNITSALCTGWVAGSLHY"
     gene            complement(18854..19636)
                     /locus_tag="VBC74_000612"
     CDS             complement(18854..19636)
                     /locus_tag="VBC74_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4931 domain-containing protein"
                     /protein_id="HEO6941583.1"
                     /translation="MCYNNSMNERYIRFNPNIAKQKPSNKDKCPFCDKSQLGKILDVK
                     DDMIWVENKYPTLEETYQTLVIESNDHNGDISVYSESKMRQLLDYLLSKWQLMEESGH
                     YRSVVLYRNFGPLSGGSLRHPHSQIVGLKNVDAYETLNLDWFDGIEVCSETSQQAEIS
                     IANRPMLDFTEFTIKVSKLENAILIADSLPILCHYILNEFLGGRCKSYNLFFYKHDGG
                     LICKIVPRFIVSPYQIGYAIHQVNLPDELEKIKEALRAKYYL"
     gene            19795..20064
                     /gene="rpsN"
                     /locus_tag="VBC74_000613"
     CDS             19795..20064
                     /gene="rpsN"
                     /locus_tag="VBC74_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEO6941584.1"
                     /translation="MAKKSKIAKFQKQQKLVEQYAELRRELKEKGDYEALRKLPKDSN
                     PNRLKNRDLIDGRPHAYMRKFGMSRINFRNLAYKGQIPGIKKASW"
     gene            complement(20425..21450)
                     /locus_tag="VBC74_000614"
     CDS             complement(20425..21450)
                     /locus_tag="VBC74_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEO6941585.1"
                     /translation="MLHFENDYNKGAHPELLNALIETNDQGLSGYGTDSYCQQAADKI
                     REVCSCPQAEVEFLVGGTQTNQVVISSMLASYEGVIAAETGHVSSHEAGAIEFSGHKV
                     LTLPSHNGKLLASEVATYIETFYGDGNYQHMVFPGMVYISHPTEYGTLYSKAELEELS
                     KICKHYQIPLFIDGARLGYGLAAKDTDVDFPTIAALSDVFYIGGTKMGALAGEAVVFT
                     KKNRPKQFTTIVKQHGALLAKGRLLGLAFDRFFTDNLYLKIGKHAIDLAEELKIILEE
                     KGYSFYLKSPTNQQFIIVENTKLADLAKNVAYSFWEKYDDHHTVIRLATSWSTSREDV
                     TALRNVL"
     gene            complement(21570..22580)
                     /gene="tsaD"
                     /locus_tag="VBC74_000615"
     CDS             complement(21570..22580)
                     /gene="tsaD"
                     /locus_tag="VBC74_000615"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEO6941586.1"
                     /translation="MKDRYILAVESSCDETSVAILKNDKELLANIIASQVESHKRFGG
                     VVPEVASRHHVEVVTTCFEDALQEAGIVASDLDAVAVTYGPGLVGALLVGMAAAKAFA
                     WANKLPLIPINHMAGHLMAARDVKELQYPLLALLVSGGHTELVYVSEPGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMIKEDHLEFSFSGLKSA
                     FINLHHNAEQKGEALVLEDLCASFQAAVLDILLAKTQKALLKYPVKTLVVAGGVAANQ
                     GLRERLATDISPDIDVVIPPLRLCGDNAGMIALAAAIEFEKENFASLKLNAKPSLAFE
                     SL"
     gene            complement(22656..23063)
                     /gene="rimI"
                     /locus_tag="VBC74_000616"
     CDS             complement(22656..23063)
                     /gene="rimI"
                     /locus_tag="VBC74_000616"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEO6941587.1"
                     /translation="MESSEQALAIWSVLSDVYDKSPWSLSQISEDLKKDSTDYFFVYN
                     DGEVIGFLALQQLVGEVEITNIAVKKNYQGKGYAYQLMSMIADIEVPVFLEVRYSNIV
                     AQKLYERCGFVVLRKRKNYYHDPIEDAIVMRKG"
     gene            complement(23065..23757)
                     /gene="tsaB"
                     /locus_tag="VBC74_000617"
     CDS             complement(23065..23757)
                     /gene="tsaB"
                     /locus_tag="VBC74_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEO6941588.1"
                     /translation="MMKVLAFDTSSKALSVAVLNNMECLATVTINIKKNHSINLMPAI
                     DFLMQSIDLEPQDLDRIVVAEGPGSYTGLRVAVATAKMLAYTLKIDLVGVSSLYALTN
                     GFSENDLLVPLIDARRNNVYVGFYQNGDTVKPDCHTSLEEVLQEVGNKANVHFVGEVA
                     AFFDQIKKALPHAKITETLPCAVAIGRKGQKMKSVNVDAFVPRYLKRVEAEENWLKNH
                     CETNTEEYIKRV"
     gene            23936..24166
                     /locus_tag="VBC74_000618"
     CDS             23936..24166
                     /locus_tag="VBC74_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEO6941589.1"
                     /translation="MIYKVFYQETKERNPRREQTKTLYVTIDAANELEGRIAARKLVE
                     ENTAYNIEFIELLSDKHLEYEKETGVFELTEF"
     gene            24220..25899
                     /locus_tag="VBC74_000619"
     CDS             24220..25899
                     /locus_tag="VBC74_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO6941590.1"
                     /translation="MSNINLKPEEVGVYAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVENIDRIKALVITHGHEDHIGGIPFLLKQANLPIYAGPLA
                     LALIKGKLEEHGLLRDATLYEIHANTELTFKNLSVTFFRTTHSIPEPLGIVIHTPQGK
                     VICTGDFKFDFTPVGEPADLHRMAALGEDGVLCLLSDSTNAEVPTFTNSEKIVGQSIM
                     KIIEGIEGRIIFASFASNIFRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PKGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKINNIHTSGHGGQQEQKLMLRLIKPKYFMPVH
                     GEYRMQKVHAGLAVDTGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRHDLSEDGVVLAVATVDFDSKMILAGPDILSRGFIYMRESGDLIRESQH
                     ILFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDK"
     gene            complement(26057..26566)
                     /locus_tag="VBC74_000620"
     CDS             complement(26057..26566)
                     /locus_tag="VBC74_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6941591.1"
                     /translation="MEENMNIKQLKSKTMLGTVALVSAFSFASTNADANTYNYAVDVD
                     YLASAEEIAQAHPASNTFPLGQCTWGVKEMATWAGNWWGNGGDWAASAASAGYTVGTQ
                     PRVGSIVCWTDGSYGHVAYVTAVDPVTNKIQVLESNYAGHQWIDNYRGWFDPQNTVTP
                     GVVSYIYPN"
     gene            complement(26714..28060)
                     /gene="glnA"
                     /locus_tag="VBC74_000621"
     CDS             complement(26714..28060)
                     /gene="glnA"
                     /locus_tag="VBC74_000621"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEO6941592.1"
                     /translation="MTITAEDIRREVKEKNVTFLRLMFTDILGVMKNVEIPATDEQLD
                     KVLSNKAMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     EPFAGDPRGNLKRNMKRMQEMGYKSFNLGPEPEFFLFKMDENGNPTLDVNDKGGYFDL
                     APTDLADNTRREIVNVLTQMGFEVEASHHEVAVGQHEIDFKYDDVLKACDNIQLFKLV
                     VKTIARKHGLYATFMAKPKFGINGSGMHCNMSLFDNEGNNAFFDPEDPRGMQLSEDAY
                     YFLGGLMKHAYNYTAIINPTVNSYKRLVPGYEAPVYIAWAGRNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALSVLLGSGLEGIENKIEAPEPIETNIYAMTVEERRQAGIVDL
                     PSTLHNALKALEEDEVVKAALGTHIYTNFLDAKRIEWASYATYVSQWEIDNYLDLY"
     gene            complement(28094..28465)
                     /locus_tag="VBC74_000622"
     CDS             complement(28094..28465)
                     /locus_tag="VBC74_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6941593.1"
                     /translation="MKERELRRTMAVFPIGAVMKLTDLTARQIRYYEDQGLITPERTE
                     GNRRMFSLNDMDRLLEIKDFISDGLHISDIKNEYMQRQHKSKEKQKSLSDAEVRRLLQ
                     DELRNQGRFSSPSQHIGNMHL"
     gene            complement(28545..>28728)
                     /locus_tag="VBC74_000623"
     CDS             complement(28545..>28728)
                     /locus_tag="VBC74_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEO6941594.1"
                     /translation="KSGIIGGTAALLIITLSIPSGETILYVFARIFETFCGVFIAMMV
                     NTDIEILRKKLKNNKL"
BASE COUNT         9529 a         5641 c         4548 g         9010 t
ORIGIN      
        1 tacattccaa ctaaacctaa cgaagctttt gaagagctca aaggactagg ttttccatta
       61 aacaaaaaga attctgataa agctaatttg gaagcttttc tcagacattc ttttttaaat
      121 caaactgata ctgattacgc tcttagtctc cttatcgttg atgcaaaaac cgatgctcta
      181 acctttttta aatcaaatag tgacttaaca ctagaaaatt tacaatggat ttatttacag
      241 ttattaggct ttatcccttt tgtagacttt aaagacccta aagcattctt acaagatatt
      301 aacttcccag tctcatatga caatattttt caaagtctac atcacttact cgcctgtcgt
      361 ggaaaatctg gcaatacatt aatcgaccaa ttagttgctg atggtttact tcatgcagat
      421 aatcactacc attttttcaa tgggaagtct ctggccactt tcaatactaa ccaattgatt
      481 cgcgaagttg tctatgttga aacatcctta gatactatgt ctagtggtga acatgattta
      541 gtaaaagtta acattatcag acccactacc gagcatacta tccctacgat gatgacagct
      601 agcccctatc atcaaggtat caatgatcct gccgcagacc aaaaaacata ccaaatggag
      661 ggtgcgctag cagttaaaca acctaaacac atacaagttg acacaaaacc atttaaagaa
      721 gaagtaaaac atccttcaaa attacccatc agccctgcaa ctgaaagctt cacacacatt
      781 gacagttata gtctcaatga ctattttctt tctcggggtt ttgctaatat atacgtttca
      841 ggtgtgggta ctgctggctc tacgggtttc atgaccagtg gggattacca acaaatacaa
      901 agctttaaag cagtcattga ttggttaaat ggtaaggtta ctgcattcac aagtcataaa
      961 cgagataaac aagtcaaggc taattggtca aatggccttg tagcaaccac aggtaaatct
     1021 tatcttggta ccatgtcaac tggtttagca acaactggcg ttgaggggct gaaagtcatt
     1081 atcgctgaag ccgcaatctc cacatggtat gattattatc gagaaaatgg gcttgtgtgt
     1141 agtccaggcg gctaccccgg tgaagattta gacgttttaa cagaattaac atactcacga
     1201 aacctcttag ctggtgatta catcaaaaac aacgattgct atcaagcatt gttaaatgaa
     1261 caatcaaaag caattgaccg tcaaagtggg gattacaacc aatactggca tgaccgtaat
     1321 tacctaactc acgtcaataa tgtcaaaagt cgagtagttt acactcatgg actacaggat
     1381 tggaatgtta agccaagaca tgtctataaa gttttcaatg cattgcctca aaccatcaaa
     1441 aaacaccttt ttttacatca aggtcaacat gtgtatatgc ataattggca gtcgattgat
     1501 tttcgtgaaa gcatgaatgc cttactaagc caagaactac ttggcattga caatcatttc
     1561 caattagaag aggtcatttg gcaagataat actactgagc aaacttggca agttttagat
     1621 gctttcggag gaaaccatca agagcaaatt ggtttaggtg atagtaaaaa acttattgat
     1681 aaccattatg acaaagaagc ctttgatact tattgtaaag acttcaatgt gttcaaaaat
     1741 gatcttttca agggaaataa taaaaccaat caaatcacta ttaatcttcc tctaaagaaa
     1801 aattatctcc tgaatggaca gtgcaaactc catctacgtg ttaaaactag tgacaaaaag
     1861 gccattttat cagcccaaat cttagactat ggtcctaaaa aacgattcaa agatacacca
     1921 accatcacat tcttaaacag ccttgataat ggtaaaaatt ttgccagaga agctttacgt
     1981 gaactcccgt ttactaaaga tcattatcgt gtcatcagta aaggtgtctt gaaccttcaa
     2041 aatcgtacag acttacttac aattgaggct atcgagccag aacaatggtt tgatatcgag
     2101 tttagcctcc aaccaagtat atatcaattg agtaaaggtg ataatctaag gattatcctt
     2161 tatacaactg attttgaaca taccattcga gataatgcta gttattctat aacagtagat
     2221 ttaagtcaat cttatttaac tatcccaact aatcaaggaa attaacttat gaaacttctc
     2281 actaaagaac ggtttgatga ttctcaacac ttttggtacc agatcaattt attacaagag
     2341 agtaacttcg gagcagtttt tgaccatgat aataaaaaca ttccacaggt tgttgcaact
     2401 attgttgatg atttacaagg ttccggaagt tcgaattatt tctggtattt tggcaatact
     2461 actgatactt ccatccttat gattgctcat ttaaatcgaa aattctatat tcaggttaat
     2521 ttaaagaact ttgactttgc actcaattta atagctataa ataattggaa gagtctcctc
     2581 caaactcaac tcgaagctct aaacgatacc ctagcaatat ttcaataaat aaggtagaat
     2641 ggagtgacaa agcaacgcga gggagactga ttaatgtcat cttattggaa taactatcct
     2701 gaacttaaaa aaaatattga tgaaaccaat caactaattc aagaaagaat acaggtcaga
     2761 aataaagata ttgaagcggc gctaagccaa ctcacagctg cgggaggaaa acagctcaga
     2821 ccagcattct tttacctttt ttctcaactt ggtaataagg agaatcaaga tactcagcaa
     2881 ctaaagaaaa tcgctgcttc tttagaaatc cttcacgttg ctacattaat ccatgatgat
     2941 gtcattgatg actcaccact aagacgtgga aatatgacca ttcaaagcaa gtttggcaaa
     3001 gacatcgcag tttacactgg ggatttactt ttcacagtct ttttcgatct tattttagaa
     3061 tctatggctg atacaccatt tatgaggatt aatgcaaaat ctatgcgtaa aattctcatg
     3121 ggagaattgg accagatgca ccttcgttac aatcaacaac aaggtatcca tcactattta
     3181 cgtgcgattt caggtaagac agccgaactc tttaaattag ctagcaaaga aggagcttac
     3241 tttggtggtg cagagaagga ggttgttcgt ctagcaggcc atatcggatt taacattggt
     3301 atgacattcc aaattttgga tgatatcctg gattatactg cagataaaaa aacatttaac
     3361 aagcctgtct tagaggattt agcacaaggc atttacagcc ttcctctact tcttgccatt
     3421 gaagaaaatc ctgatatttt caaacctatt ttagataaaa aaacagatat ggctactgaa
     3481 gacatggaaa aaattgctta tctcgtcgtt tcccatagag gtgttgacaa agctcgccat
     3541 ctagctcgta aatttactga aaaagctatt agtgacataa ataagctacc ccagagctct
     3601 gcaaaaaaac agttgctaca attaactaat taccttttaa aacgcaaaat ttaaataata
     3661 aaaaaacatt ccacaatgct agaaaagcag ttagggaatg tttttttatt atcatttatt
     3721 tatcgcacct atcaatcatc atagatcacc atcatcagcg gctttcagct gacggtaacg
     3781 ttggctactt tgagacaatt cttgaggaga accttccaac tctaattgcc cattttctat
     3841 aaataagata cgatcagcat gttcaatacc ttttaagtga tgtgtaatcc aaactaaggt
     3901 cttaccttcc aattctttca taaataccct tagtaaggct tgttcagtaa taggatcaag
     3961 tccaacagtt ggctcatcta agataacaat tgggacatct tttagtaaga ttctagccaa
     4021 agcaattcta tgcctttcgc cacctgaaaa cctaagtcca gcttcatcaa ccattgtata
     4081 gagaccatct gataaatcag tgaccatctc tttcaatcca actcgttcaa gaactttcca
     4141 tacatcttct tcactagcat cttggtttcc aatgcgaatg ttatttagca gggttgtatt
     4201 aaaaaggtag ggcgcttgtt gtatcactcc aatatagtta gaaatgcaat caccaactat
     4261 tgaaacatca gcaccgccta gggtaatctc cccttgactt gctttcaagt cgccacgaag
     4321 tagactggct aaggtactct tgccagaacc actacgccct aaaatagcaa ttttttctcc
     4381 ttctttaata tccaaatcta aatgatgcaa aacccatttc tcttgtggct tatactggaa
     4441 acttaaattc ttgacggaaa aatcgtatgg cttattaggc aactgatttt gagttgtttc
     4501 aaaatacgtt tctgataatt cattaagacg atgaattgaa tcagaatatt tatttgtttc
     4561 ttgagcagca gcagacaaac cagcaaatgc ctctgacaag ggaaatactg ttagaacaaa
     4621 tgctgcaatc cagttagctt cgccacctct gtgaccaata aactgattac tagcccaaat
     4681 aataaccaaa atagctagaa agccaaaaac taattccaca ataagagctc tacgattatc
     4741 aaaggaacga atttttttct gaacagccat gagttcactt tccgaacgct catgcaaggt
     4801 gacgtactct tgaccacgtt gactaaaaat ccagtctgaa atacctaaga cattatccgt
     4861 caaatcagta taaagatgat tttttaattc tttttcacga gtctgacgag caccattagc
     4921 taaaatcgac caaagtggaa aaagaaaaat cataatagct aaataaagca acatcattaa
     4981 agcaaaccat agagaaaaga agcctgttgc aataataata aagctatata atatccacgc
     5041 tataatcgtt ggaaatattg tcctaagata caaattttgc aaataattaa tatcctccgc
     5101 taaaagcccc ataacatctc ctaaacgaaa atcacgcttc ataaagatag cattgctttc
     5161 taaggaatgg taaagcttca agcgcagctg tgaagtcatt cgtaataccc aattgtgact
     5221 cgttaggcgt tctatataac gaaaaacagg acgtccaatg ccaaaagcac gtgttagtac
     5281 aattggaaca tatactagaa gaatattact cggtagtgaa gctgacttac taatcagata
     5341 gcccgaatta aacataagag cactggcaga aaagaaagtc ataaaaccaa ggaaaagagc
     5401 aattactaag gaaactttat attgtttgaa aaatggtttt atccattgat ctgtctttaa
     5461 ttctttaaac aaagggattt taaacatcat ttcctcccat agcatgcttc agttgataga
     5521 gatatccttt tttagctaat aattcctgat aagagcctac ttctgcaact ctccccttct
     5581 ccataacgac tataacatcc atttggttca accagtgaag acgatgagtt gcaaagataa
     5641 ctaaacgatc tgacatcagt ggcaacattt tctcttttaa ttctaactct gtttcaatat
     5701 ctaaatgagc agttggttca tcaaaaacca taatacgacg attttgatca agaaaagcac
     5761 gagccagagc tatacgctgg gcttgtcctc cactaagcgg acgcgctcca ttaccaatac
     5821 gcgtttctaa accgtcaggt aactcgctta gcaagctatc taaacctacc atatgaatcg
     5881 ctcttaccac ctcttcgtca ctagcattgg gagtatagaa ggtaatatta tcacgtaatg
     5941 acatttcaaa aacataagga gtttgaggaa tatagatcat ctgtttatgc caatcttctt
     6001 gatcaaggtt agtcacttct ctaccatcca cttttaaaga accattatca ggccctaaaa
     6061 aaccactcag aaggttaatt aagctagact tacctgaacc actaactcct actaaagcaa
     6121 ccttttgatg tcctctaaat cgtaaggtta catcctcaac aacacgtttt tggtcgtacg
     6181 caatagcaat gttttctaaa tccaaacgac tctctttaga ccaatcatct atgactaatt
     6241 gctcaccttt aataccagtt ttgtttagaa ctttttgaat cgcttgaaat gcatttttcc
     6301 cgtctaacgt tgcatgataa tcactagaaa aatctcttac gggtaaaaag tattcagggg
     6361 ataaaatcaa aattgttaaa gccggtaaaa ggtaaatctg ctcatttaat aatcgtaaac
     6421 ctagaaaaac agcaacgata gcaatggata aagttgtaaa aaagtctaaa gcaaacgttg
     6481 ataaaatacc aattttcaaa gtactcattg ttgctttacg aaaagattca ctagtttgat
     6541 aaatgctttt accataacgt ttactaagac caaaatattt gagagtatca atgcctcgta
     6601 atgaatctaa aaaatgatta gaaagtactt gataggattc atattgttta tctgctttag
     6661 cttgagccgc atagcctaaa ataatcataa agatgataat taaaggaaaa acgataagta
     6721 aaattgcacc tgattcaata tctaaataga aaatataagc tagaataatc catgggataa
     6781 tagacatgtt aatcatctta ttcaagacta aacgtaaata attttcaact aaagaaacgc
     6841 catctaatgc cattgtaaca acattaccag ttccttgttc ttgaacgact ttaggaccta
     6901 gatcaaataa cttatctaat aattgacgtc tcaaaagact agattgtgct gttgaaaagt
     6961 catcaagttt tctatttttg atgtaatcaa taacatgacg gcctaaatat gagaccataa
     7021 ataacaaaac ataaaccgtt tgagaagaaa gtttttgccc ttcccacaaa ccagttatag
     7081 aaagactcaa ataataagct tgtccgataa taaagattgc ttgaagaaca tcaagtcccg
     7141 caagcaatcc taacaactta tgaatacctg acaagcgcat aaccgcttta tctagcatta
     7201 tttttctcct gtcacaatca ctggcaaggt aatacgtttt ctaaagatat aatatgccca
     7261 tgctgtataa gctagaacaa agggaactag agtaattgcg acaatcgaca ttacttttaa
     7321 agtatatggc gttgaggatg cattttgaat cagcaaatca tatttagggc taattgatga
     7381 aatcataact cgtgggaata gaccttggaa caacagaaca accactgaaa caagactgag
     7441 accactcgct aaaaatgctg tcatctctgc tcctttaaaa gttgaagcat gcgctaatac
     7501 agctaataca acaataacca aaaccataat tgtcgtaact atcggatgat tgctaaagaa
     7561 atctgtcttg aaatacaaga gaactgcaaa caaggctaaa ccaagataga gtacccaata
     7621 aagcaactga gcataattac gggcacgctc tctaattggc ccttctgtct tcaatgcaat
     7681 gtaatttaaa ccatgaagat aggctagcaa taccattgca actccaccca ccaacgagaa
     7741 tatattaaag taatcactga attgagctga taagttacca ctggcatcaa gtggcatacc
     7801 ttgaatcaaa cttataaaca taatcccaaa gaagaatgga acaatagcgg aaccaattgt
     7861 taaggtccag ttccaaaact gctttttctc tgcaggaact ttatgtctaa attcgaagga
     7921 aacaccacga ataattaatc caaagagaat cgtaaggaga atcaaatagt aaccactaaa
     7981 tagtgaagca taccagtatg gaaaagaagc aaacattgct ccaccaccgg taagaagcca
     8041 aacttcatta ccatcccaaa ctgggccaat ggtttctact acttgatctt tttcatgctc
     8101 attatgtgtt aaagtttgaa cagccatccc aaccccaaag tcgaatcctt caaggaagaa
     8161 gaatcctgaa aataggaggc cgattaggaa aaaccaaaag aattgtaaag cactcattta
     8221 gtatgctcct ttctcaaagg gatcaattga agtatatgca ccatgatgtc cgtgttcatc
     8281 ttcatactct ggccctttct tcaattctct tattgttagg cttatcatca tcaccgctaa
     8341 accaccaaac aacaagaaat acacagtatt ggaaacaaat aatgatgcaa ctgaaacgtt
     8401 tggagagaca gagtccttaa tcttgaaaag accatagaca gtccaagggt atcgtccttg
     8461 ttctgtcaca atccaaccaa atgtattggc taagaatggt gcaaaggttg ttaaagcaac
     8521 aatccaaagc atccatttgt gtttataaag aattggtttt ttcttacgag ttaaaaagag
     8581 tcctaaaaca gaaacaccca taatagctgt tcccatcgcc gccattgtac ggaaaccata
     8641 gaaaagtagg ttaaccattg gatagtagtt atctttacca tacttagcaa ccaattcttt
     8701 attagctgta tccatacctt tcacagaacc agatggtttc ccgtaagata aaatactcaa
     8761 catataagga atcttaatcc caaaaacttg tttgtgttct gcttcatttg cccaagcaac
     8821 tacactccaa gctgcaggat cacccgaatc ttcataatca ccttccatag cagcaaattt
     8881 cattggttga tcgtgaatca aggctttcat ttgtaaatcc ccaactccca taactgaaat
     8941 tgaaccaaga agtgctacta ctaaaccaat acgaaccgat ttcttataaa gttcaaccgt
     9001 tgtatctttc agctgttctg attttaagag gcggaaggct gacataccag caaccactgt
     9061 accacccatc gtaatggcac caaaaataac atgggtaaat tcatagaaga actgtggatt
     9121 ttttacaagt gcaagaaaat cagtcatttg cgctctaccg tttacaactt cataaccaac
     9181 aggatgttgc atgaagctat tagcagtaag aatccacatc gctgacatta atgagccaaa
     9241 aaccactagc caaatgaatg tcacatgtaa ttttttacta atttttttgt tatcccatgt
     9301 aaacatccat aaaccaagga aagtagattc cataaagaaa gctaataaag cttcaatagc
     9361 caatggagct ccaaaaatat cacctacgaa acgtgaatag tctgaccagt tcattccaaa
     9421 ttggaactct tgaatgatac ctgtcacaac accaaccgca aaactaagga gcatgatatt
     9481 accccaaaat tttgtcaatt ttttatactc ttcatttttt gtgataacat acattgtctc
     9541 cataatagca accactaggc atgtaccaat tgtaaatggt acaaagaaaa agtggaaaac
     9601 tgtagtcatc gcaaattgaa aacgagctaa agtttcaatt gtcatacaaa acctcttatt
     9661 ttgttttaat catttctttt atagaaatat ctgcaagagc tagtttccta gcttcgtaac
     9721 aattggtaaa taacttactt taatatctta atgatataaa tcaaaacgtc ccttagctag
     9781 aagttcttta agtccaccca tatcaacaag tgatttgttc atgataatct tcttcataac
     9841 cgacgcaggg tattttttaa ttttagtttt tccaacaaca ccaagtccat gcgtatttcc
     9901 tactgaagca actgtgcctt gcggactata cgaaaagtct tctgtagctt cacctttgat
     9961 ttgatgtaaa aggtttttag caacatgagc tcccatacga gttgcaattt gagctgttgt
    10021 tgggaatggg cgaccagact cagtgtccat aaatgcagac acatcaccga taacataaac
    10081 attatcgtat tttggatcac gcaaatcact gttgaccatt acacgtccac gacgttgatc
    10141 aaatcctgat tctcccataa ccggacttcc actaacacct gttgtccaaa tgatagtacc
    10201 tgcggtaata gatttgagtt cttcatcctc tttactagta ccataaacaa cttcacccgg
    10261 tttgatttcc ttaatcatac ttccaagcat caaattaata cctaaatcct taattaagtt
    10321 gacaccatat tgcgctaatt catcatcaaa cattggtagg atgcgagttg ctgcctctac
    10381 acaaataatt tcaatttgtt caggttttac acccgctaaa gcagcatatt ttggtcgctc
    10441 atcaaccata gctccggcta actcaatacc tgtaaaacca gcaccacata ctaagagttt
    10501 gagaagattt tcatcttttg tctcacggta caacttcatt gtattaacaa tgtgattatg
    10561 gatgttttct gcttgcgaaa tattggtcat ctgcaaagca ttctccatag cacccttgat
    10621 gccaaaagtt tcagatacaa agcctaaggc aactacgaca taatcataat ggagttcacc
    10681 gttattttta actgttactg ttttattttc agcatcgact ctaagcacct catcttgcat
    10741 gaagttaacc ttattagtgt tgataacatc cttaattggg aatgtaattt tttcttttgg
    10801 ctgagagcct gcagctactt catgtaattc tgtagcttcg tagtgataat cattcatgtc
    10861 aacaagggta atatgaaaat cacctgattg tttttgtaaa ttacgaactg ctttcaaacc
    10921 ggcataacca gcacctaaaa ctaggatttc tttcatgata aaactcctct aaaaaatgat
    10981 tccaattaga tacgttaaga cttgggttgc agatcccagt gctagaatac gtaccgcaca
    11041 aacaaatgtt tctctcttaa cctgtttttg ccacaatagt tttgtctgct tataaataaa
    11101 tggtattaat aacaaactca agagtactgt aacaggcgca agtccaaaaa gaaattcaaa
    11161 aacaattgcc aataaagcta tactattaga aatagcaaac agcaagagtc cacctcttat
    11221 ccctgtataa tgtacaaggg tatagcgatg atttttttca tcttcttctt tgtcgcacag
    11281 attattcgcc aacattaggt tggcaatcca taaggtgttc ggcaaggaaa tcaagaaaat
    11341 tttgcttaag ttgaggatat cccacgaaaa gttttgataa gtgtttagat atacacaaat
    11401 taaactaatc ataaatccca ttgtcaagcc agaaaatact tctcctaagg gcagactaga
    11461 taaaggtctt ggaccaaagg aatataaaac accaattcca aaacaaaaaa gtcccatcca
    11521 taaaagaggc agaccgactt gggaaactaa aactaaacca atcatactag aagtaatgac
    11581 taaacttgcc ataataactt ctatttggcg tagggatagg ttctcacgac caataatgtt
    11641 agtgtcattc ttataatcca aatccacagc attccgataa tcattgtaat tattccaaat
    11701 gtcaacgaac atattgaata aaaacatagc aacgaagaat aaaccgacat atacagggtg
    11761 tactgaattg tagtaataag cactaaaaca caaaccaatc aaaaatggta aaacactagc
    11821 tgttttggct ttcatttcta ccaactctag aaagattggt aatgtcatta ttaaagtcct
    11881 ctctatacat aatcttcaac aatactatta taatgcaatt tctaccattt ttaaaacaat
    11941 agggcttgat ttttgattaa aaatacaaaa aatttttctt aaaatgagtt aatcacactt
    12001 agaacactaa atactatttt gaggagtcct attcatcaca ctatgccatg taatattcgg
    12061 aaaaatagta ttgcaaccct caactatccc ttatgttggc accagccttt gaaagtcctc
    12121 tgattgcact gcttagttag tattttcatc agctgtatga tctctaagag ccatcagcat
    12181 actaaaaaaa ctagtattac actttgaaaa gtgtaacact agctccaatt tgttttaaat
    12241 agcttaacta tttaatcatt aatctacttt atatatttaa gcatttaaag ttgaatgttg
    12301 gttgaggata cctgagagag aaccttgaat ataatttggc aatacttgtt tttgagcacg
    12361 ttcatagatt tctgaatctg ttaactgacg ttctgatttg gggcttgata cttgcggtgt
    12421 cactgctgca ttttgttgtt tgtaaagtgt tgaatgctgg ttaaggatac ctgagagaga
    12481 accttggata tattttggta atacttgttt ttgggcactt tcataaagtt ctgaatctgt
    12541 taactgacgt tctgatttgg ggcttgatac ttgcggtgtc actgctgcat tttgttgttt
    12601 gtaaagtgtt gaatgctggt taaggatacc tgagagagaa ccttggatat atcttggcaa
    12661 tacttgtttt tgggcacgtt cataaagttc tgaatctgtt aactgacgtt ctgcttttgg
    12721 aggtgttgct tgcggtgctg tgaaagttga agctgaaaca ccttgtgtag aaccaagagc
    12781 tacgaatgct cctccagcaa acattgctga gacaagacca agtttcaaat agttagcagt
    12841 tgagacgttg ttgacttttt tttctgtaat aagtttgttc ataagaatta cctctttctt
    12901 ttgggttatt tattttatga actaagtata acaacaagaa ataaagtaag gttaaactaa
    12961 atataaaata attcttaagt cattcttagg aaaccataaa atcatacact aacaaaaaat
    13021 ttggtatcta gctcaaaagt aaaagtagaa taaaggcttt tacttctgag ctagtaatga
    13081 ataaattata tttgcatcta gtttggaatt agccctagtg tattataatg atccctacaa
    13141 atatgtgcca aacaccttct tcatatatac aagattgatt taacattcct atacccttaa
    13201 tggtgggcac attctcttca tgttccatat agaaatcaac agataattcc gtattgtccc
    13261 tattattaaa ttttccagtt aatgataaat tatatcgtcc accttgaggt gtcctattac
    13321 caatctgttc aacctttaca cttgacgaat cgaattgaac agattctgtc ttatcattct
    13381 gtgatttaga acatcaacaa tttcttgttc atgttctttg aggtaagcta gttgttcttc
    13441 tttaacgtgc ttttatcttt cgtacttact gatacttgag agcatttttt tgaccctact
    13501 tcttactaat cagctgacgt ccctataaga tgaatcataa acacatagct gccactaacc
    13561 attttaacct tttatgcata cataattctt cttgatatcc cataccatta tctttagtat
    13621 aaacacttaa taaaacaagc atttaacaac taaggttaat aaaattcaat aaatctaatc
    13681 cttctactta ctttagttta tcactttttt ctctcatcct atgctataat agtaaggtat
    13741 caaaatttag gagaatatag tcttatgatg aatatgcaaa atatgatgcg tcaagcacaa
    13801 aagcttcaaa aacaaatgga acaaacgcaa gctgatttag cagctagcca gtttactgga
    13861 aaatctgctc aagagctcgt taccgtgaca tttacaggcg ataaaaagct aatttcaatt
    13921 gattacaaag aagctgtggt agaccctgaa gatattgaaa cactacaaga catgacaact
    13981 caagctatca atgacgcttt aagccaagtt gacgatgcta caaaaaaaat tatgggagca
    14041 tttgcgggta aaatgccatt ctaaaaaata aatcacattt actgaatcac ctgtaaatgt
    14101 gatttttatt tcaaacttct ttcatttttt cacgaaaaca agttttattt tcaaaaaata
    14161 tttgcaaaaa atcagcagag aggtataata acattgaaaa tattagagag aaggtgacta
    14221 accatggtta tggatagtct aatcataaaa cagttgatta agagtacatt tgatataccc
    14281 ttacaagtca cttatccaaa tgggaatatt gaaacttata atggtagcaa tccccacgtc
    14341 aaacttaaac taaacaaaaa tttctccgtc tcggagctat caaaagaccc ttccatagta
    14401 ttgggtgaag ctgttatgga tggagacatt gaaatttatg gttctataca agaattaatt
    14461 ttatcagctt accgctgtgg agacagcttt ctacgtaata gtaaatttag caaattgatt
    14521 cctaaacaat ttcatgataa aaaacacagt aaatccgaca ttcaaaagca ttatgacatc
    14581 ggaaatgatt tctataaact gtggcttgat gataccatga cttattcatg cgcctatttt
    14641 aaacatgaaa atgatagtct agagcaagct caactaaaca aagtacacca tattcttaat
    14701 aaattaaatg ctcagccggg agggaaatta ctagacatcg gttgtggctg ggggacactt
    14761 atcattactg ctgctaaaga atatggctta aatgcgacag gtatcacctt aagtgaagag
    14821 caggcttctt tcattactaa gcgtatcaag gaagaagggt tggaaaataa agtaacagtc
    14881 ctcattaaag attatcgtga cattcgtgaa acatacgatt atatcactag tgttggtatg
    14941 tttgaacatg taggaaaaga aaatttatct caatacttcc aaaccatttc taaaagactc
    15001 aacatcaatg gtctagctct tattcatggt attactggac aagtgggtgg taatcatggt
    15061 agtggaacta attcttggat taataagtat atcttcccag gaggatatat ccctcgttta
    15121 actgaaaatt taaatcatat agctagtgct ggacttcaaa ttgctgactt agaaccgctt
    15181 cgtcgtcact atcaaaagac tctcgaatta tggacaaaaa atttccacaa tgcccttcct
    15241 gaagttcaga aaactcatga taagcgtttt atcaatatgt gggacctcta tcttcaatct
    15301 tgtgcggcct cttttgaatc tggcaatatt gatatttttc aatacttgct ttcaaaaggt
    15361 gtttcaaaag acactatgcc tatgacacgc gattatatgt attctgctaa ttgatatagt
    15421 taatattata gtattccaac ttattctaaa taaaaaggac tctcttgaat tcaagggagt
    15481 tctttaacgt tcttctctgg ctaactctat acaatgtgac ttttggtgac aatgtggaca
    15541 gaatactttt cgcgtcttag gggtatgttt cgctagatta aatgcttgat atgtcggtgt
    15601 aaaggtttga tgacaatttg gacaaagata aacaacctgt tgtttaaaat accaaaccat
    15661 accattcata gctattaaga atactgttac tgctatccac ataagccatc tttgatttag
    15721 agaaactgct gctattatta acgtaatata gaccataata atcataacga cttgtagcac
    15781 tatgcttttt tgtagtatac cccattttct tttgttttct tccataagca caatgtcaga
    15841 aagatagcct aatgttttac tatcttgttt ttctacagtt tttagtaggt tgacagcttt
    15901 gtcaagtttg tcttttttac tatcaatagc tagccttaat tctcttatct acacttgtaa
    15961 aaaaagctct aaaatctggc tagcattgtc ttcagtaaat agatttttga tattatcaat
    16021 tgaaaaatct aaatctcgta aataaataat ttgtctaagc ttttctaaat cagcaatact
    16081 ataaaccctc ctacctcctt cagatagttc tgatggggat aaaattcctc ttttgtcata
    16141 gtactgaaca gtacgaacag taactcctgc ttctttagct aagtcaccag tggaatattt
    16201 ttctgacatg tttcacacct cctttaatat agtaaacact tctttatcag aaacaccttt
    16261 actatatcaa atgacctaag gtaataagca agtaataacg ctaagtattt ttaactttaa
    16321 ataaaactac aacaattaat caaacaaatt tcctaaagtc gcaaatgggc tatcaactgc
    16381 tacctctttt tgctgtttta aatagatttt attttcatcc aagtccagaa atttttcata
    16441 aacacgcgca gtcatacggg catcctctaa gctattatgt gctttaccct ttattcctaa
    16501 aaagtcagca acagtagtca atttaaggtt gacaatacca tttaaatctg tactgcgtct
    16561 cacaaaagct tcatcatata agtccacctg atattgacta gttagatcca aaccattttc
    16621 aactaaaagg ggtaaatccg atttataccc attataacca atcagtggtg tatctccaac
    16681 aaaactctga aagtctgtca aaactatctc aattttagga gcccctataa tatctctagc
    16741 tgtaatacct gttaaaccat taataaagct ttgtaatgga acttttgtgt gaacataagt
    16801 atcaaataaa gcaatctctt gatgattaga atatttaacg gccgacactt gaataatatg
    16861 gctatgctca ccaaccgtgt taaactctaa atcaaaagct atatattctt gtaattgttt
    16921 cattatttct cctaaaaagc gcaaggaaac tccttacgct tttattctat tttttattga
    16981 agaaacgtcc aaagaaactt tttttaacag cttctaattc ttgaattttt tcttctttgg
    17041 cttcttgaat ctcctcaacc tctgctttag cttcatttaa ctcaagttgc agacgttgcg
    17101 tatcttccat agcagttaaa gtcaattgct gttgttgatc taattgctta tctttctctg
    17161 ctatttgaat atcttttaca tgtaactgct cgtcttttga agcaatctga atatcttttg
    17221 cttttaactg ttcatataaa cgtgtgattt cagtattctt ctcatcaacg agaatttcta
    17281 aaagttctcg tcttgatgct tcttcgtcaa ttggttcctc ttccaaaata gtttttttat
    17341 agatttcttc caatttaatc aagccatcac gattaacaac tgtaacgcct ttactattct
    17401 tttccacaca ttcttcaggg agtgttttaa ctctgttatt catcgcttga cgactaaccc
    17461 ctaaaatttc agctaattca ctaaccgtct tttcaactgt cataacttcc tcttttgtac
    17521 tgattttctt ataaaagaat atcacaatcc taatctagtg tcaatttact gtcaacttaa
    17581 agtgtcaaac aagacatttt gacgtctatt tctctattta tatgctagaa taagattatg
    17641 aagcattatg acacaataat tataggtggt ggacctgctg gaatgatggc agctatttcc
    17701 tcaaattttt acggcaacaa aacacttctt attgagaaaa ataaacgttt aggcaaaaaa
    17761 ctagctggaa caggtggtgg ccgctgtaat gttaccaata atggaaacct tgatgagctc
    17821 ttagctggta tacctggaaa tggccgtttc ctatatagcg tcttctcaca atttgataac
    17881 catgatatta ttaacttctt tcaagacaac ggcgtcacac ttaaagaaga agatcatggg
    17941 cgtatgttcc ctactactga taaatctcgt actatcatta acgcccttga aaataaaata
    18001 aaagaacttg gtggtcaaat tatgactgac acagaagttg tctctgtcaa aaagatagga
    18061 gattcattct atatcaaaac taaagataca cagttcgcat ccgataaact tatcgtaaca
    18121 actggtggta aatcctatcc gtctacaggt tctactggtt tcggtcatga cattgctcgt
    18181 cactttaaat tagaagtcac agatatggaa gccgccgaaa gccctctttt aactgatttt
    18241 ccccataaaa aactacaagg tatctcctta gatgatgtca ctttgtcatt tgaaaagcat
    18301 attattacac acgatcttct ctttactcat tttggattat caggaccagc agccttgcga
    18361 atttctagct ttgtcaaagg cggagaaact atatacctag atgttttgcc caatatctct
    18421 gttaaagaat tggaaataca cttccaaaat gaaagagaaa aatctttgaa aaacgctctt
    18481 aaaatacttc ttcctgagcg tttagctgaa ttttacgctg aagacttacc cgaaaaagta
    18541 aaacaagtat ctgtaaaaga cttagaaatg ttaattcaaa aacttaaaaa acttcctatt
    18601 ttagtaactg gcaagatgtc tctagcaaaa tcctttgtaa caaaaggggg agttgatctt
    18661 aaagaaataa accccaaaac acttgaaagt aaaaaggtag ctggtttaca ttttgctggt
    18721 gaagtcctag acatcaatgc tcatacggga gggtttaaca ttacttctgc cttatgtaca
    18781 ggctgggtag ctggtagctt acactattaa tacatgaaag ctgaacaatt ccgtcagctt
    18841 ttttatatag aatttaaaga taatatttgg ctcttaaggc ttctttgatt ttttccaatt
    18901 catctggtaa atttacctga tgaatagcat acccaatctg ataaggggat actataaaac
    18961 gaggaacaat tttacaaata agacccccat catgcttata gaaaaagaga ttataagatt
    19021 tacagcgccc tcccaagaat tcatttaaaa tataatgaca caaaattggt aaactatctg
    19081 caatcaaaat agcgttttcc aatttggata ctttaattgt gaactctgtg aaatcaagca
    19141 taggacgatt ggcaatacta atctctgctt gttgactcgt ttccgaacaa acctcgatac
    19201 catcaaacca atctaagttc aacgtttcat aagcatctac gttttttaag ccaacaattt
    19261 ggctatgagg atgtcgtaaa cttccgccag ataagggccc aaaatttcga taaagaacga
    19321 ccgaacggta atgaccactt tcttccatta attgccattt tgacaaaaga taatctaaca
    19381 attggcgcat ctttgactca ctataaactg aaatatcccc attgtgatca tttgattcaa
    19441 tcaccaatgt ttgataggtt tcttctaaag ttgggtattt attttctacc cagatcatat
    19501 cgtctttcac gtctaggatt tttcctaatt ggcttttatc acaaaatgga catttgtctt
    19561 tattgctggg tttttgtttc gcgatattag ggttaaatcg gatatatctt tcattcatac
    19621 tgttattgta acacattcat caccattaca atataatact tatccagtta acattttcct
    19681 tgcattctta gctgaaaaga gtataataga atcatgtctt gtttaccggt taattaccag
    19741 ttaactaggc accctgtttt gggatagaaa aatctttttg ggaaaggaga aatcatggct
    19801 aagaaatcaa aaattgctaa atttcaaaaa cagcaaaaac tcgtagaaca atatgctgaa
    19861 ctacgtcgtg agttaaaaga aaagggtgat tacgaagcac ttcgtaaact tcctaaagat
    19921 tctaacccta accgacttaa aaatcgtgat ttaatcgatg gccgtcctca cgcttacatg
    19981 cgtaaattcg gtatgagtcg tattaacttc cgtaacctag cttacaaagg ccaaattcct
    20041 ggcattaaaa aagcaagctg gtaattcgtg aagacaaata gttacttttt atgactattc
    20101 tattgagtgt ggacgaatag ttaatacatt gtaattattt aacctgttga gtatttactc
    20161 agctattaca aacttcttca aaattttatt atcataggaa tgtaggatct catcaatcct
    20221 taaatgacta ataataaaag atgaaaaaat ttttcttatc tcaaatcata gatgccaatt
    20281 tatttatttt caaaaagccc ggtatgccaa gataccgagc tttttattat tataaatact
    20341 gataaaattc ttttagttaa tcccaaaaac aagactatta ttttgtaata gtcactactg
    20401 aataatattt cttttattaa aaactcacaa gacatttctt aaagcagtta catcttcgcg
    20461 actagttgac caactagttg ctagacgtat tacagtatga tggtcatcat atttttccca
    20521 aaaactataa gctacattct ttgctaaatc tgctagtttt gtattttcta caataataaa
    20581 ttgttgatta gttggtgatt ttaaatagaa tgagtaacct ttttcttcta aaatgatttt
    20641 taattcctct gctaagtcga tcgcatgctt tccaattttt aagtaaaggt tatccgtaaa
    20701 gaaacgatca aaagcaagcc ccagcaatct tcccttggct aataacgcac catgctgctt
    20761 tacaattgta gtaaactgtt ttggacgatt tttctttgtg aaaacaactg cttccccagc
    20821 aagagccccc attttggttc caccaatata aaaaacatct gataaggctg caatcgttgg
    20881 gaaatcaaca tctgtatctt tcgcagccaa accatacccc agacgagcac cgtcaataaa
    20941 taaggggatt tgataatgtt tacaaatctt cgataactct tctagttctg ctttactata
    21001 aagtgttcca tattctgttg gatgtgatat ataaaccatt cctggaaaaa ccatgtgttg
    21061 gtaattacca tcaccataaa aagtctcaat ataagttgcg acttcgcttg cgagtaattt
    21121 cccattatga gaaggtaaag tcaatacctt atgccctgaa aattctatgg ctcctgcttc
    21181 atgactactc acatgacctg tttctgcagc aataactcct tcataagaag caagcatact
    21241 actgataact acttgatttg tttgagtacc acccactaaa aactcaactt cagcttgtgg
    21301 acaggaacag acttctcgaa ttttatctgc cgcttgttga cagtaactat ctgtcccata
    21361 tcccgataga ccttgatcat tagtttcaat aagggcattt aaaagctctg gatgagctcc
    21421 tttattataa tcattttcaa aatgtaacat tccttaactc ctttattagt taaaaaaata
    21481 gcgacaatat catttgctaa tatttttgaa tattcaatca ttataacaaa taaaaaatat
    21541 caaaacaagc gttttgatat tatgaacacc tataaactct caaaagctaa actaggtttg
    21601 gcattcaatt ttaaagaagc aaaattctct ttttcaaact ctatcgctgc tgctaatgca
    21661 atcattcctg cattatcccc acataatcta agaggaggaa taaccacatc aatatcagga
    21721 gaaatatcag tagccaagcg ttcccgaagt ccttgattag ctgcaactcc accagcaacg
    21781 actaaagttt tcactggata ctttaacaaa gctttttgag ttttggccaa taaaatatcc
    21841 aaaacagccg cctgaaagga agcacataaa tcttcaagaa ccaatgcttc acccttttgt
    21901 tctgcattat gatgtaaatt gataaatgca gattttaatc cagaaaaaga aaattcaaga
    21961 tgatcttctt tgatcatcgc tctaggaaaa tggtaagtat cttgaccctt atgagctaac
    22021 tgatcaatct cgcgacctgc tggataagtt aagcccataa cacggcctac tttatcataa
    22081 gcttctccaa cagcatcatc ccgagtttct cctactattt tgtaatctcc cggttcagaa
    22141 acatatacta attctgtatg tcccccactg acaagcaaag ctaacaatgg gtattgaagt
    22201 tccttaacgt cacgtgctgc cattaaatga cctgccatgt ggtttatagg aattagaggt
    22261 aatttatttg cccaagcgaa agcttttgct gcagccatac ctaccaataa ggctcctact
    22321 aatcccggac catatgttac agcaacagca tccaaatcgc tagcaacaat acctgcttct
    22381 tgaagagcat cctcaaaaca ggttgttact acttcaacgt gatgacggct tgccacttca
    22441 ggaacaacac caccaaaacg tttgtgactt tcaacttgac ttgcaataat attagctagt
    22501 aactctttat cattttttaa aatagcaaca ctagtttcat cacatgaact ttccactgct
    22561 aaaatatatc tatctttcat aatgatatta atccatcaat atcgtctttt ccattagtat
    22621 tgaggactat tcatatcctt tctatcatca actatttatc ccttgcgcat cactatagca
    22681 tcttcaattg gatcatggta ataatttttg cgctttctta aaactacaaa accacacctc
    22741 tcataaagtt tttgtgctac gatatttgaa taacgaacct caagaaaaac tggcacctct
    22801 atatcggcga tcattgacat taactggtaa gcataacctt taccttgata atttttttta
    22861 acagcaatat tagtaatttc tacttcacct accaattgtt gaagagccaa aaaaccaatg
    22921 acttcaccgt cattataaac aaaaaaataa tctgttgaat ctttttttaa atcttctgaa
    22981 atctgcgata aactccacgg agatttgtca tagacatcag atagtacgct ccatatagcc
    23041 agtgcttgtt ctgatgattc catgtcaaac tctcttaata tattcttctg tattcgtttc
    23101 acagtggttt tttaaccaat tttcctcagc ttcaacacgt tttaagtatc gtggaacaaa
    23161 cgcatctaca ttaacgcttt tcattttttg tcctttgcgc ccaattgcta ctgcacaagg
    23221 taaagtttct gtaattttag catgtggtaa ggctttctta atctgatcaa aaaatgctgc
    23281 aacctctccg acaaaatgaa cattggcttt attccccacc tcttgtaaga cttcttcaag
    23341 agaagtgtga cagtctggtt taacagtatc accattttga tagaaaccaa cataaacatt
    23401 gttacgtcgt gcatctataa gtggtaccaa taaatcattt tctgaaaatc catttgttaa
    23461 agcgtacagg ctagatactc caactaagtc aatcttaagc gtataagcta gcatttttgc
    23521 tgtagcaaca gctacacgta agcccgtata agatcctgga ccctctgcta ctacgatacg
    23581 gtccaaatct tgaggttcta aatcaattga ttgcattaaa aaatcaatgg ctggcatcaa
    23641 attaatgcta tgattctttt tgatattgat agtgacagtc gctaaacatt ccatattgtt
    23701 tagtacagcc actgatagtg ctttgcttga agtatcaaag gctaaaactt tcatcattta
    23761 acttccctta cttgattcta cctcttattt tatgatataa tcatgataat tgcaacgaat
    23821 caaggttaat tctattactc tataaaaact taataatggt ttgtcattgt ctcacttaac
    23881 ggcattgacc cattttttta tagttagaaa aaccgagaga taagaaagga ttattatgat
    23941 ttataaagtt ttctatcaag aaacaaaaga acgcaaccca cgtcgtgagc aaaccaaaac
    24001 actttacgtt actattgatg cagctaatga attagaaggt cgcattgctg cacgtaaact
    24061 agttgaagaa aatacagctt acaatatcga attcatcgag ttactttcag acaaacacct
    24121 tgaatatgaa aaagaaactg gtgttttcga gctcactgag ttctagaaag gagttaaaca
    24181 taatagctag agtaatctac ccattatgat aaacttatta tgtcaaatat caacttaaaa
    24241 cccgaagaag ttggggttta tgcgattggt ggtctcggtg aaatcggaaa aaatacttac
    24301 ggtatcgaat atcaagacga aattattatt gtagacgctg gtattaaatt ccctgaagat
    24361 gatcttttgg gaatagatta tgttatccca gactattcat atatcgttga aaacattgac
    24421 cgtatcaagg cccttgttat aactcatggg cacgaggacc acatcggtgg tattcctttt
    24481 ctattaaaac aagctaattt accgatttat gcaggtcctc tcgctcttgc tctaatcaaa
    24541 ggtaagttag aagaacatgg tttacttcgt gacgctacat tatacgaaat ccacgctaat
    24601 acagagctga cttttaaaaa cttaagcgtt acatttttcc gcactactca ctccattcct
    24661 gaaccattag gtattgttat tcatacccct caaggtaagg ttatttgtac aggtgacttc
    24721 aaatttgact ttacacctgt tggggagccc gctgacttac atcgtatggc tgctctaggt
    24781 gaagatggtg tcctctgcct tttatcagac tcgacaaatg ctgaagttcc aaccttcact
    24841 aattccgaaa aaattgttgg gcaatctatt atgaagatta tcgaaggtat cgagggacgt
    24901 attattttcg cctcttttgc ttcaaacatc ttccgactcc aacaggctgc tgaagctgct
    24961 gttaaaactg gtcgtaaaat tgctgttttt ggacggtcaa tggaaaaggc aattgttaac
    25021 ggtattgaac ttggctatat taaagttcca aaaggtacct tcattgagcc aagcgaactt
    25081 aaaaatcttc atgctagtga agttttaatt atgtgtacag gtagtcaagg tgagtctatg
    25141 gcagcacttg ctcgtatcgc taatggtact catcgtcaag ttactttgca gcctggtgat
    25201 actgtgattt tctcatcaag cccaatccct ggtaatacaa caagtgtcaa taaattaatc
    25261 aataccattc aagaagctgg tgtcgatgtt attcatggta aaattaacaa tatccatacc
    25321 tccggccatg gtggtcaaca agaacaaaaa ttaatgttac gcttgattaa accaaagtat
    25381 ttcatgcccg tacacggtga gtatcgcatg caaaaagtcc atgctggttt agcagttgac
    25441 acaggtattc ctaaagagaa tatcttcatt atggaaaacg gtgatgtttt agctttaacg
    25501 agcgactctg ctcgtattgc tggacatttc aatgctcaag atatctacgt tgatgggaat
    25561 ggtattggcg atattggtgc ggcagttcta cgtgaccgcc acgacttatc tgaagatggt
    25621 gttgtccttg ccgttgctac cgttgatttc gattcaaaaa tgattttagc tggaccagac
    25681 atccttagcc gaggctttat ctatatgaga gaatctgggg accttattcg cgaaagccaa
    25741 catatcttat tcaatgctat tcgtattgca ctgaaaaata aagatgcaag tatccaatca
    25801 gtaaatggag ctattgttaa tgccttacgc ccattcttat atgaaaaaac tgagcgtgag
    25861 cctatcatta ttcctatggt ccttacccct gataaataaa caaaagaacg gaaaatactt
    25921 tctccgttct ttttaattgc ctatattagc aaaataagtt aatatatcaa attacttctg
    25981 ggataactat agaatataaa agaactccaa acagtatcgt acttttatac cgtctatgtg
    26041 aggagttctt tatagattaa ttcggataaa tgtagctaac tacccctggt gttactgtat
    26101 tttgtggatc aaaccaacca cgataattat caatccattg atgacctgca taattagatt
    26161 ctaaaacctg aattttatta gtgacaggat ctacagctgt aacataagcc acatgtccat
    26221 aactaccatc agtccaacat acgatagaac ccacgcgcgg ttgtgtccct actgtataac
    26281 cagctgatgc ggcgctcgca gcccaatcac caccgtttcc ccaccagtta cctgcccatg
    26341 ttgccatctc ctttacaccc catgtgcatt gacctagtgg aaacgtatta ctagctggat
    26401 gtgcttgagc aatttcctcc gcacttgcta ggtagtctac atctactgca tagttatatg
    26461 tattcgcatc tgcatttgtt gaagcaaatg aaaaagcgga caccaaagcc actgttccaa
    26521 gcattgtttt agactttaat tgttttatgt tcatgttttc ctccatctga tgactcctag
    26581 tttatcaaat ccatataaca gacaaatgaa aacctgtaac aagttttagg caatttttta
    26641 caataattta caaaaaaact agttttagac tttacaaaat aaagcctaaa actagttata
    26701 aacattctta tgattaatat aaatctaggt aattatcaat ttcccattgt gaaacataag
    26761 tcgcataact tgcccattca atacgcttag cgtctaagaa attagtatag atatgagttc
    26821 ctagggccgc ttttaccact tcatcttctt caagtgcttt aagagcatta tgaagagtag
    26881 atggtaaatc aacaatacct gcttgacgac gttcttctac agtcatagca tagatatttg
    26941 tctcaattgg ttctggtgct tcaattttat tctcgattcc ctcaagtcct gatcccaaaa
    27001 gtactgataa agcaagataa ggatttgctg ttgggtcaac cgagcgcaat tctaggcgtg
    27061 ttcccatacc tcgagatgca ggtacacgaa taagtggcga acgattgcga cctgcccaag
    27121 caatgtaaac tggtgcttcg tatcctggta ccaaacgttt ataagaatta acagttggat
    27181 taatgatagc tgtgtaattg taagcgtgtt tcattaaccc acctaaaaag tagtaggcat
    27241 cctctgataa ttgcattcca cgtggatctt ctggatcaaa gaaagcattg ttcccttcat
    27301 tatcaaaaag agacatatta caatgcatac ctgaaccatt gataccaaac ttaggttttg
    27361 ccataaaagt agcatataaa ccatgcttac gagcaattgt tttaacaacc aatttaaata
    27421 attgaatgtt gtcacaggct tttaaaacat catcatattt aaagtcaatt tcatgctgtc
    27481 caactgctac ttcatgatga ctagcttcaa cttcaaagcc catttgtgtt aagacattaa
    27541 caatttcacg acgtgtatta tctgctaagt ctgttggagc taagtcaaag tagcctcctt
    27601 tgtcattaac atcaagtgtt gggttaccat tttcatccat tttaaatagg aaaaattcag
    27661 gttctggtcc taagttaaat gacttatagc ccatctcttg catacgtttc atattacgct
    27721 taagatttcc acgtggatct ccggcaaaag gttctccttc tgctgtatag atatcacaga
    27781 ttagacctgc aacagctcca ttttcatctc cccaaggaaa gacaatccaa gtatctaaat
    27841 caggatataa atacatatca gattcattga tacgaacaaa gccctcaata gaagatccat
    27901 caaacattgc tttatttgat aaaactttgt ctaattgttc atctgtagcc ggaatttcga
    27961 catttttcat tactcctaag atatctgtga acatcaaacg gaggaaggta acattttttt
    28021 ccttaacttc gcgacgaata tcttctgctg tgatagtcat atagtctcct taatatctag
    28081 cttgactgca tgtttacaaa tgcatatttc caatgtgctg tgaaggactt gaaaaacgcc
    28141 cttgattacg gagctcatct tgtaaaagtc gacgaacctc ggcatctgat aatgactttt
    28201 gtttctcttt ggacttatgt tgtctttgca tgtattcatt tttaatatca gaaatatgca
    28261 aaccatcgct aatgaagtct tttatttcca gtaagcggtc catatcattc agtgaaaaca
    28321 tgcggcggtt accttcagta cgttcaggtg tgattaaccc ttgatcctca taatagcgaa
    28381 tttgtctggc agtcaaatct gtcaacttca taacagctcc aatcgggaat acagccattg
    28441 tacgcctaag ttctctttct ttcatgatgg cctcctttca ttaaaccatt ataaacttta
    28501 aaaagtttga tgtcaactct catgtgagat tttatgacat caaactataa tttgttattt
    28561 tttagttttt tccgaagtat ttcaatatct gtattaacca tcattgcaat aaaaacacca
    28621 caaaaagttt caaaaatacg tgcaaaaaca tatagaattg tctctccgct tgggatagat
    28681 aaagttataa taagtaatgc ggctgttccc ccaataatac ctgattta
//
LOCUS       DATROD010000014        28343 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.014, whole genome shotgun sequence.
ACCESSION   DATROD010000014 DATROD010000000
VERSION     DATROD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28343
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.014"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..1200)
                     /locus_tag="VBC74_000624"
     CDS             complement(<1..1200)
                     /locus_tag="VBC74_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEO6941595.1"
                     /translation="MGLFGFGKPKDEALEAKISSLESKLKDKQSILDDLTRQIKVAKD
                     ILKLDDELKKRQSELKALQKEVIVLNDDLDLQEYGFFERKYKFSDSTKYKEELETLRK
                     RQKQLVKDKKAGVIVRPMLLDNSKSKGRAMQNQLIKAMIRGFNGEVDAHLVKVTATNL
                     SKKVDAVVKSFNQLNKMYSRNLVCLSNDYLELKISELQLAVEYELQKLEEKELLREQR
                     AKEREDKKLQAELAAKRKQIEKDRKHFKQMLDNVEELLKTANEEEKEKLNLQLAEYQD
                     KLSELDELEEDIDYREGHATAGYVYIISNIGAFGEDVYKIGVTRRLDPMERIRELGSA
                     SVPFQFDVHALIFSEEAFSLEKELHNRFEKNKINKVNGRKEYFKVPFSDIKAVLEEHK
                     ELAIELTE"
     gene            complement(1210..2283)
                     /gene="purK"
                     /locus_tag="VBC74_000625"
     CDS             complement(1210..2283)
                     /gene="purK"
                     /locus_tag="VBC74_000625"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEO6941596.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGHKVITLDPASDCPASRV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENVDADGLDAVVSAGQLPQGTDLLRISQNR
                     IFEKDFLANKAGVTVAPYKVVTSSLDLEGLDLTKTYVLKTATGGYDGHGQKVIRSAED
                     LPEAQQLANSAECVLEEFVDFDLEISVIVSGNGKDVTVFPVQENIHRNNILSKTIVPA
                     RISDQLADKAKKMAVQIAKELQLSGTLCVEMFVTADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGQPLPPIKLHDPAVMLNVLGQHVQQAIDYVAQNPSAHLHMYGKLE
                     AKHNRKMGHVTVFVEDADEVEEF"
     gene            complement(2270..2758)
                     /gene="purE"
                     /locus_tag="VBC74_000626"
     CDS             complement(2270..2758)
                     /gene="purE"
                     /locus_tag="VBC74_000626"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEO6941597.1"
                     /translation="MQPIISIIMGSKSDWTTMQKTAEVLDNFGIAYEKKVVSAHRTPD
                     LMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSNE
                     LI"
     gene            complement(3044..4306)
                     /gene="purD"
                     /locus_tag="VBC74_000627"
     CDS             complement(3044..4306)
                     /gene="purD"
                     /locus_tag="VBC74_000627"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEO6941598.1"
                     /translation="MKLLVVGSGGREHAIAKKLLVSKGVDQVFVAPGNDGMTLDGLGL
                     VNIVISEHSRLIDFAKENEIAWTFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTYSDFEKAKAYIEEQGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFANGDKFYIMPTAQDHKCAY
                     DGDMGPNTGGMGAYAPVPHLPQSVVDTAVETIVKPILEGMITEGRPYLGVLYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGAKFAENGKALLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDTTGLFYRNDIGSKAIK"
     gene            4588..5391
                     /locus_tag="VBC74_000628"
     CDS             4588..5391
                     /locus_tag="VBC74_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEO6941599.1"
                     /translation="MTIYDQIESALDLMTDLEHEIACYFMGQPISKDALASSIVTKQL
                     HISQAALTRFAKKCGFKGYREFVFEYLKSHETISQQLYGLQNDNTKKVFMNYQEMVSK
                     SADIIDEEQLLEVSHMIEQADRVYFYGKGSSSLVAKEFKIRLMRLGVICEALDDTDSF
                     SWTNSIVNDRCLVIAFSLSGNTNSVIGALKIASCHGAKTVLFTKQPHTIDYAFDKIIQ
                     VASARHLDYGNRISPQIPMLIMVDIIYAQFLDINKIEKERIFRETIIQR"
     gene            complement(5414..6391)
                     /locus_tag="VBC74_000629"
     CDS             complement(5414..6391)
                     /locus_tag="VBC74_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEO6941600.1"
                     /translation="MIETMSLDDMREYLGQDQIPEDFDDFWKKQTMKYQGNIEYRLDK
                     KDFNITFAQAYDLHFKGSNNSIVYAKCLFPKTNKPYPVVFYFHGYQNQSPDWSDQLNY
                     VAAGYGVVSMDVRGQAGQSQDKGHFDGITVKGQIVRGMISGPNHLFYKDIYLDVFQLI
                     DIIATLESVDSNQLYSYGWSQGGALALIAAALNPKIVKTVAVYPFLSDFRRVLDLGNV
                     SEPYDELFRYFKYSDPFHKTENNVLKTLAYIDVKNFAHRISCPVVLLTALKDDICPPS
                     SQFAIFNRLTSTKKHLLLPDYGHDPMTVQVKDYIFDQLTGSQFTKQKIE"
     gene            complement(6399..7280)
                     /locus_tag="VBC74_000630"
     CDS             complement(6399..7280)
                     /locus_tag="VBC74_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEO6941601.1"
                     /translation="MTRTVAIDIGGTMIKHGIVDNLGCIVEASELATEAYKGGPGILQ
                     KVCQIIDNYLTEGSIDGIAISSAGMVDPDEGCIFYSGPQIPNYAGTQFKKVLEDTYQV
                     RTEIENDVNCAGLAEAVSGSAKDSSIALCLTIGTGIGGCLIIDKTVFHGFSNSACEVG
                     YMHLSDGDFQDLASTTALIADVAKAHGDEINRWDGRRIFQEAKEGNEKCIASIDRMIN
                     YLGQGIANMVYVVNPEKVVLGGGIMAQKDYLQDKLSESLKRNLVTSLAEKTIIVFAQH
                     ENQAGMLGAYYHFKNRG"
     gene            complement(7297..8214)
                     /locus_tag="VBC74_000631"
     CDS             complement(7297..8214)
                     /locus_tag="VBC74_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEO6941602.1"
                     /translation="MKDLQKYQGIIPAFYACYDDKGDICPERVKALTNYFIDKGVQGL
                     YVNGSSGECIYQSVADRKLVLENVMSVAKGKLTVIAHVACNNTKDSVELAMHAEAMGV
                     DAIAAIPPIYFRLPEYAIADYWNTISQAAPQTDFIIYNIPQLAGVALTSDLYRKMLQN
                     PQVIGVKNSSMPVQDIQNFVAIGGENHIVFNGPDEQFLGGRLMGAAAGIGGTYGVMPE
                     LYLTLNQLIVDKDLEKARELQFTINDIITKLCSGHGNMYAVIKAVLEINEQLTIGSVR
                     LPLASVTEEDKPIIKEAAEMIRHAKKQFC"
     gene            complement(8211..8873)
                     /locus_tag="VBC74_000632"
     CDS             complement(8211..8873)
                     /locus_tag="VBC74_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEO6941603.1"
                     /translation="MKKANQLIAAIFDLNNPFMQGCNVVFDLALLNLLFMITCLPLVT
                     IGAAKISLYRTLWQKLEGDQTNLLILYIKHLKKEWFQGMLLGLVELSILVVIIFDLTI
                     LHYQIGFIVPFLKIICYAFLLLTVMTSIYLFPMAARYEMSLLDTVKKSFIMACLNLKW
                     TGVLMFLLIMTWFIMVQSSLLFMLTVSAIFIFAYTAFAYFKIIILQKQFAYFSKQQGD
                     YQ"
     gene            complement(8893..9336)
                     /locus_tag="VBC74_000633"
     CDS             complement(8893..9336)
                     /locus_tag="VBC74_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEO6941604.1"
                     /translation="MIYDHLLNLTHYKDINPNLDLAIDYLLSHDLRNLDIGTYHISPE
                     VILMVQSNQLSESFDHIFEYHKKYLDIHYVIEGYEVIKLGKGDKVEVEEYLGDIGFVK
                     CSEETSFDLRDNYIAFFFPEEAHQPNGMGSLGNYVKKGVLKVLMA"
     gene            complement(9349..10179)
                     /locus_tag="VBC74_000634"
     CDS             complement(9349..10179)
                     /locus_tag="VBC74_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEO6941605.1"
                     /translation="MKKKTFSAYNFLTALILCLLTVLFIFPFYWIMTGAFKSQPDTII
                     IPPQWWPKAPTLENFKALTVQNPALRWLWNSVFISIMTMFLVCCTSSMAGYVLAKKRF
                     YGQKILFSLFIAAMALPKQVVLVPLVRIINFMGIHDTLWAVILPLVGWPFGVFLMKQF
                     SENISTELLESAKIDGCGEIRTFINVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            complement(10189..11076)
                     /locus_tag="VBC74_000635"
     CDS             complement(10189..11076)
                     /locus_tag="VBC74_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO6941606.1"
                     /translation="MRTNKLKMRETMIAYAFLAPILLFFLIFVFAPMVMGFVTSFFNY
                     SMTQFTFIGLANYNRMFHDSIFMKSLINTVIIVIGSVPVVVFFSLFVAANTYEKNVFS
                     RSFYRCVFFLPVVTGSVAVTVVWKWIYDPMSGILNYILKSGHVIEQNISWLGDKHWAL
                     LAIIIILLTTSVGQPIILYIAAMGNIDNSLCEAARVDGANEMQVFWQIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIALISFAQFKILGNDVEY"
     gene            complement(11164..12480)
                     /locus_tag="VBC74_000636"
     CDS             complement(11164..12480)
                     /locus_tag="VBC74_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6941607.1"
                     /translation="MSIKKSVIGFCLGAAALSMFACVDSSQSVMAAEKDKVEITWWAF
                     PTFTQEKAKDGVGTYEKKVIKAFEKKNPNIKVKLETIDFTSGSEKITTAIEAGTAPDV
                     LFDAPGRIIQYGKNGKLADLNDLFTDQFIKDVNNKNIIQASKSGDKAYMYPISSAPFY
                     MAFNKKMLKDAGVLKLVKEGWTTSDFEKVLKALKNKGYTPGSFFANGQGGDQGPRAFF
                     ANLYSAPITDKEVTKYTTDTKNSVKSMKKIVEWIKKGYLMNGSQYDGSADIQNFANGQ
                     TAFTILWAPAQPKTQAKLLESSKVDYLEVPFPSEDGKPDLEYLVNGFAVFNNKDENKV
                     KASKKFITFIADDKKWGPKDVIRTGAFPVRTSFGDLYKGDKRMMKISKWTQYYSPYYN
                     TIDGFSEMRTLWFPMVQSVSNGDEKPADALKDFTQKANDTIKKAAK"
     gene            complement(12527..13225)
                     /locus_tag="VBC74_000637"
     CDS             complement(12527..13225)
                     /locus_tag="VBC74_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEO6941608.1"
                     /translation="MPHLSKEAFKKQIKNGIIVSCQALPGEPLYTESGGVMPLLALAA
                     QEAGAVGIRANSVRDIKEIQEVTNLPIIGIIKREYPPQEPFITATMTEVDQLATLDIA
                     VIALDCTLRERHDGLSVAEFIQKIKRKYPKQLLMADISTFEEGKNAFEAGVDFVGTTL
                     SGYTDYSRQEEGPDIELLNKLCQAGIDVIAEGKIHTPKQANEINHIGVAGIVVGGAIT
                     RPKEIAERFISGLS"
     gene            complement(13472..14776)
                     /locus_tag="VBC74_000638"
     CDS             complement(13472..14776)
                     /locus_tag="VBC74_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO6941609.1"
                     /translation="MKMNKKVLLTSTMAASLLSVASVQAQETDTTWTARTVSEVKADL
                     VKQDNKSSYTVKYGDTLSVISEAMSIDMNVLAKINNIADINLIYPETTLTVTYDQKSH
                     TATSMKIETPATNAAGQTTATVDLKTNQVFVADQKVSLNTISEGMTPEAATTIVSPMK
                     TYSSAPALKSKEVLAQEQAVSQVAANEQVSPAPVKSITSEVPAAKEEVKPTQTSVSQL
                     TTVSPASVAAETPAPVAKVAPVRTVAAPRVASAKVVTPKVETGASPEHVSAPAVPVTT
                     TSPATDSKLQATEVKSVPVAQKAPTATPVAQPASTTNAVAAHPENAGLQPHVAAYKEK
                     VASTYGVNEFSTYRAGDPGDHGKGLAVDFIVGKNQALGNEVAQYSTQNMAANNISYVI
                     WQQKFYSNTNSIYGPANTWNAMPDRGGVTANHYDHVHVSFNK"
     gene            complement(14923..15822)
                     /locus_tag="VBC74_000639"
     CDS             complement(14923..15822)
                     /locus_tag="VBC74_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEO6941610.1"
                     /translation="MNKWLVKASSLVVLGGMVLSAGSRVLADTYVRPIDNGRITTGFN
                     GYPGHCGVDYAVPTGTIIRAVADGTVKFAGAGANFSWMTDLAGNCVMIQHADGMHSGY
                     AHMSRVVARTGEKVKQGDIIGYVGATGTATGPHLHFEFLPANPNFQNGFHGRINPTSL
                     IANVATFSGKTQASAPSIKPLQSAPVQNQSSKLKVYRVDELQKVNGVWLVKNNTLTPT
                     GFDWNDNGIPASEIDEVDANGNLTADQVLQKGGYFIFNPKTLKTVEKPIQGTAGLTWA
                     KTRFANGSSVWLRVDNSQELLYK"
     gene            complement(16015..17562)
                     /gene="purH"
                     /locus_tag="VBC74_000640"
     CDS             complement(16015..17562)
                     /gene="purH"
                     /locus_tag="VBC74_000640"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEO6941611.1"
                     /translation="MTKRALISVSDKSGIIDFAKELKNLGWDIISTGGTKVALDDAGV
                     ETIAIDDVTGSPEMMDGRVKTLHSNIHGGILARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDVTNDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
                     SQTTFKTRQRLAAKAFRHTAAYDALIAEYFTAQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAEKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEVTALEFAWKAIKYVKSNGIIITNDHMTLGLGVGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQESIDAANKHGLTMIFT
                     GVRHFRH"
     gene            complement(17582..18334)
                     /locus_tag="VBC74_000641"
     CDS             complement(17582..18334)
                     /locus_tag="VBC74_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6941612.1"
                     /translation="MKLVKNLEIVESIFGDWDETIIWSCVQGIMGEVFVDSLDQPKSS
                     LAKLGRKSSFGFLAGQPTLFLLEVCSGEDIILVPQHKGWSDLIESTYGQNAHSFKRYA
                     TKKDTLFERSRLEKFVTQLPNGFELRVIDEKVYNSCLEKEWSQDLVANYATYQYYKKQ
                     GIGYVIYYQGNIIAGASSYSTYKNGIEIEVDTHPDFRRRGLATIVAAQLILTCLDKGI
                     YPSWDAHTRTSLNLSEKLGYEFSHEYIAYEID"
     gene            complement(18357..18905)
                     /gene="purN"
                     /locus_tag="VBC74_000642"
     CDS             complement(18357..18905)
                     /gene="purN"
                     /locus_tag="VBC74_000642"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEO6941613.1"
                     /translation="MKIAVFASANGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQAVVDLLDKHEIDLVCLAGYMKIVGETLLSAYEGRIINIHP
                     AYLPEFPGAHGIKDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPDVLDSLGIKRK"
     gene            complement(19072..20094)
                     /locus_tag="VBC74_000643"
     CDS             complement(19072..20094)
                     /locus_tag="VBC74_000643"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEO6941614.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVSRTERAGVMGALGGFG
                     GVFDLSKTGVREPILVSGTDGVGTKLMLAIKYQQHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYIATGKNNPVKLEEVVSGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQLLDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGKELLPELEGKQLK
                     DVLLEPTRIYVQAALPLIKEELVNGIGHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     DSVVIK"
     gene            complement(20122..21576)
                     /locus_tag="VBC74_000644"
     CDS             complement(20122..21576)
                     /locus_tag="VBC74_000644"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEO6941615.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNPSFMGKVKE
                     ALSTVKGGFAYLLMTEDKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDSGIQCDRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     RLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAADEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEETSFYIQKVK"
     gene            complement(21810..25535)
                     /locus_tag="VBC74_000645"
     CDS             complement(21810..25535)
                     /locus_tag="VBC74_000645"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEO6941616.1"
                     /translation="MNKRIFVEKKADFDIKSASLVKELTHNLQLTSLKDLRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDCLLTETEITAELDKVAFFAIEALPGQFDQRAASSQE
                     ALLLLGSDSQVKVNTAQLYLVNKDIAEAELEAVKNYLLNPVDSRFKDITLPLEEQAFS
                     VSDKTIPSLDFFETYKADDFAAYKAEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQATYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRDGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVRPSDVDTFIA
                     ACNKENIDAVVVATITAKPNLVMTWDGETIVDLERRFLDTNGVRVVVDAKVVDKDLTV
                     PEVRTTSAETLEADTLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQHGVTTTASVMAQGYNPYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFDQPVSALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAAGENIYYIPGQAISEDIDFDLIKANFSQFETIQAQHKIT
                     AASAVKYGGVLESLALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIADSVKIG
                     QTQAAFTLTVNGNDLAGASLLSVFEGKLEEVYPTEFEQADALEEVPAVVSDTVIKAKE
                     TIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVPFVTLNEAAIADSVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNKKVRAAIDSFIEKGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLVGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQALFASAVKYFTGK"
     gene            complement(25658..26362)
                     /locus_tag="VBC74_000646"
     CDS             complement(25658..26362)
                     /locus_tag="VBC74_000646"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEO6941617.1"
                     /translation="MTNQLIYKGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNMAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVTAGSFSK
                     RFGVEEGRVLETPIVEFYYKNDDLNDPFINDEHMKFLGIVNDEEIAYLKGETRRINEL
                     LKDWFAQIGLNLIDFKLEFGFDKDGKIILADEFSPDNCRLWDAEGNHMDKDVFRRDLG
                     SLTDVYQVVLEKLIAL"
     gene            complement(26486..26725)
                     /locus_tag="VBC74_000647"
     CDS             complement(26486..26725)
                     /locus_tag="VBC74_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085641.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO6941618.1"
                     /translation="MSRDEVFEKMLELLRQQLGDPQLDITPESSLHDDLAIDSIALTE
                     FIINLEDVFHLEIPDEAVEHMSSVQQLLDYIIEHK"
     gene            complement(26736..27728)
                     /gene="plsX"
                     /locus_tag="VBC74_000648"
     CDS             complement(26736..27728)
                     /gene="plsX"
                     /locus_tag="VBC74_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEO6941619.1"
                     /translation="MKKIAVDAMGGDYAPKAIVEGVNQAISDFSDIEVQLYGDQKKIE
                     KYLTVTERVSIIHTEEKINSDDEPAKAVRRKKQSSMVLGAKAVKDGVAQAFISAGNTG
                     ALLAAGLFVVGRIKGVDRPGLMSTMPTLDGVGFDMLDLGANAENTASHLHQYAILGSF
                     YAKNVRGIEVPRVGLLNNGTEETKGDSLHKEAYELLAAEPSINFIGNIEARDLMSSVA
                     DIVVTDGFTGNAVLKTMEGTAMNIMGSLKSSIKSGGVKAKLGALLLKDSLYQLKDSMD
                     YSSAGGAVLFGLKAPIVKCHGSSDSKAVYSTLKQVRTMLETQVVDQLVDAFTDE"
     gene            complement(27806..>28343)
                     /locus_tag="VBC74_000649"
     CDS             complement(27806..>28343)
                     /locus_tag="VBC74_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6941620.1"
                     /translation="ICHWLSGFVEISSSNSFSFGPFFFLLLAYFIQSLTEEILFRGYV
                     MTTVTKFKGSFAGVLCNSMLFSFIHFRNYGITAIALFNLFLLGIIFSILFNMTKNILF
                     VTGVHTAWNFTMGCVLGNKVSGGYSPVSLFRITENSSFAIWSGGDFGFEGGVLCSLIL
                     VGCLAYFSLERVKVSVRY"
BASE COUNT         8492 a         6172 c         4971 g         8708 t
ORIGIN      
        1 ttcagtcaac tcgatagcaa gttctttatg ctcctctaac actgctttga tgtcagaaaa
       61 tgggacttta aaatactctt ttcgcccatt aactttatta attttatttt tttcaaatct
      121 gttatgtaac tctttttcta atgaaaaagc ttcctcacta aaaattaagg catgtacatc
      181 aaattgaaat ggaacagatg cactccctaa ttcgcgaata cgttccatag gatctagacg
      241 ccttgtaaca ccaatcttgt aaacatcttc tccaaatgca ccaatatttg aaatgatata
      301 aacatatcca gcagttgcat gcccttctcg ataatcaata tcttcttcta actcatctag
      361 ttctgataat ttatcttggt attctgctaa ttgaagattt aatttttcct tttcttcctc
      421 attagctgtt ttaagtaatt cttcaacgtt atctaacatc tgtttaaagt gttttctatc
      481 tttttcaatt tgttttctct tagcagctaa ttctgcttga agtttcttat cttctctttc
      541 cttcgcacgt tgttcacgaa gaagttcttt ttcttctaat ttctgaagtt catattctac
      601 tgctaattgt aactctgaaa tttttagctc aagatagtca ttagatagac ataccaaatt
      661 tcttgaatac atcttattaa gctgattaaa tgatttaaca actgcatcta cttttttact
      721 tagattagta gccgttacct ttaccaggtg agcatcaact tctccattaa aacctcttat
      781 catcgcttta ataagttgat tttgcattgc tcgtcctttt gatttactgt tatccagcaa
      841 cataggtctt acaataacac ccgctttttt atcttttact aactgttttt gcctctttct
      901 cagagtttcc aattcttctt tatacttagt actatctgag aacttatatt ttctctcaaa
      961 aaatccgtac tcttgaagat ctaaatcatc attaagaact attacctctt tttgtaaagc
     1021 tttaagttct gattggcgtt tcttaagttc atcatcaagt tttaaaatat ctttagcaac
     1081 ctttatctgc cttgttaaat catccaaaat agattgttta tcttttaact tactttctaa
     1141 actactgatt ttagcttcca aagcctcatc tttaggcttt ccgaatccaa atagacccat
     1201 ttattccccc taaaactcct ccacctcatc cgcatcttcc acaaacaccg ttacatgtcc
     1261 cattttgcgg ttatgtttcg cttctagttt accatacatg tggaggtgag cgctagggtt
     1321 ttgggcaaca tagtcaatcg cctgctggac atgttgtcca aggacattaa gcataacggc
     1381 tgggtcatgg agcttgattg gtgggagagg ttgccccagt acacccaaga tgtgggtgtc
     1441 aaactgtgaa aagtcacagg cttcgataga atagtgccct gagttgtggg ggcgtggggc
     1501 gatttcattg acgatgatat catctgcggt cacaaacatt tccacacaga gggttcctga
     1561 tagctggagt tccttggcaa tctgtaccgc cattttctta gccttgtcag ctagttggtc
     1621 tgagatgcga gctggtacga tagtttttga taggatattg ttgcggtgga tattttcctg
     1681 aacgggaaag accgtcacat ccttgccatt tccagacacg atgactgaga tttcaaggtc
     1741 aaagtcgaca aactcttcca agacacactc agctgagttg gctaattgtt gcgcctctgg
     1801 caggtcttct gctgagcgga taaccttttg cccatgaccg tcataaccac ctgtcgctgt
     1861 cttgaggaca taggtcttgg tcaagtcaag cccctctagg tcaaggctag acgtcaccac
     1921 cttataggga gcgacagtca ctccagcctt atttgccaga aagtcttttt caaagatacg
     1981 gttttgagaa atgcggagca gatcagtccc ctgcggtagc tgaccagctg acacaacggc
     2041 atccagacca tcggcatcaa cattttcaaa ctcataggtc aaaacatcac aacgcgcagc
     2101 taattgtccc aaagcctcaa cgtcatcgta aggcgccaca atcacctcgc taacgcggga
     2161 ggcagggcag tcgctagcag gatccagcgt aatgaccttg tggcccatgt agatagccgc
     2221 aatcgccatc atctgcccca gctgaccacc accgataatc ccaatggtct taaatgagtt
     2281 cattgcttga ctcctctgcg atttttcctt gttcctcatg gaaatgagct agcgcatcag
     2341 ccagattttg gtcctcgata gagagaatac ggagggctgt tagggcagcg ttagtcgctc
     2401 ccgcttctcc gatagccatg gttgccacag gcacaccgcc aggcatctgc acaatggaat
     2461 agagcgaatc caagcctgaa agggcacgtg atttgacagg cacaccgata acaggcaggg
     2521 ttgttttagc tgctaccata ccaggcaagt gggctgcccc acctgctcca gcaatgatga
     2581 ttttgatacc acgaccacgc gcctcctcag catgcttgaa catgaggtct ggcgtacggt
     2641 gggcagagac gactttcttc tcgtaagcaa tgccaaagtt atctagaact tcagccgttt
     2701 tttgcatggt tgtccagtcg gatttggaac ccatgataat agaaataatt ggttgcataa
     2761 tattaagata caaagggacg tttcgacttg tcgaaaattt tcgtagaaaa atatgcggta
     2821 agtccgaaat ctttgatttc gaagacgcac ccacgtcagg gcgactaggt gctttagcag
     2881 cagacgccta cgacgcaaaa atctgacgaa gatgctttag catctaggaa gatttatctt
     2941 tttcactaga aaattagtcc cgtgttcagt tcctttcttc tttctttttg gagcgacctt
     3001 cggtcgctga ttgctgacgc taatatttga atgagtagtc ttattactta atagctttgc
     3061 ttccgatgtc gtttcggtag aagaggcctg tggtgtcttg ttgggctagt tgggtataga
     3121 ttttgtcctg ccctgctttg acgctgtctt ctgtggtgac aagcatatag actcgtccac
     3181 cgtttgagag cagtgctttg ccattttccg caaacttagc tccggcatag taggtgatga
     3241 tgtcgccgtc ggtcttttca ggcagtggca cacctttctc gtaatcgagc ggatagcctt
     3301 ctgaggcaac gacaacgccc aaagtcacgc cgtccttctg ccaagtgatg taaggctcga
     3361 tgcccatcat gatgtcgtcg atgttctgag caaaatcaga agtcaggcga gggaggataa
     3421 tctgagtttc tgggtcaccg aaacgtgagt tgaactcgat aaccttcgga ccatcagccg
     3481 tcaggataag ccccgcatag aggacgccta gataaggacg cccttcagta atcataccct
     3541 caaggatagg cttaacgatg gtttcaaccg ccgtatccac tacgctctga ggcaggtggg
     3601 gaactggcgc ataggcaccc ataccgccag tgtttggtcc catatcaccg tcataggcac
     3661 acttgtgatc ctgagctgtc ggcatgatgt aaaacttgtc gccattagcg aaggcaaaga
     3721 gggagaactc ttcaccatcc aaaaattcct cgataaccac acgcgctcct gagtcgccaa
     3781 acttgttgtc caaaagcatc tcttgcgccg cctctaccgc ctgctcaacg gtttcagcca
     3841 cgaccacgcc cttgcctaac gccaagccat cagccttgac cacgattggt gcaccctgct
     3901 cttcgatgta ggctttagct ttttcaaaat ctgaatatgt gccataggct gctgttggaa
     3961 cgttgtattt aaccatgatt tccttagcaa agtcttttga ccactctagc tccgctgctg
     4021 cctttgttgg cccaaaagct ctaagtccag cactgttgaa atcatcaacg ataccagccg
     4081 ctagcgcatc atcaggacca ataaaagtcc aagcaatctc attctcctta gcaaagtcaa
     4141 tcagcctaga atgttcggaa attacgatat ttaccaagcc tagaccatcc aaggtcatac
     4201 catcattacc aggtgccaca aaaacctgat ctacaccctt agacactaac aacttcttag
     4261 caatcgcatg ctcacgacca ccagaaccaa caacaagcaa tttcatatca gaataactcc
     4321 tattaaataa tataaaaaga aatttgcaat aaaaacgtat tatttataaa attataacac
     4381 atttgttcgc ttttaagttt tttgttgcat cttttttgaa aaattaataa cataatatat
     4441 acttcacata tattatgtta ttaatccttc atattgctat ttaaaactta tcattgctgt
     4501 ttcttagaac ttagtttggc ccctcgctaa ctttatgtta tactgaatat gttaagcgct
     4561 ttcttaaaga ggagaggacc gtgcattatg actatttatg accaaattga gtctgctctt
     4621 gatttaatga ctgatttaga acatgaaatt gcttgttatt ttatggggca gcccatttca
     4681 aaagacgctc ttgcttcttc cattgtcact aagcaactac acatttcaca agcagcctta
     4741 acgcgttttg ctaaaaaatg tgggtttaaa ggctatcgtg aatttgtttt tgaatattta
     4801 aaaagccatg agaccatcag tcaacaatta tacggtcttc aaaatgacaa cactaagaaa
     4861 gtatttatga attatcaaga gatggtttct aaatccgctg atattattga tgaagaacag
     4921 cttctagaag tatcacatat gattgagcaa gctgatcgtg tttattttta tggcaaaggg
     4981 agctcatcgc tagtagctaa agaatttaaa atacgcctta tgaggctggg agtgatttgt
     5041 gaggctctgg atgatacaga tagtttctca tggactaata gtattgttaa tgatagatgc
     5101 ctcgtcattg ccttctcact ttctgggaac accaactctg ttatcggtgc cttaaaaatc
     5161 gcaagttgtc acggagctaa aaccgttcta tttacaaaac aacctcacac aattgactat
     5221 gcttttgaca agattattca ggtggcctct gctaggcatt tagactacgg caatagaatt
     5281 tcgcctcaga tcccaatgtt aatcatggta gatattatct atgcacaatt tttagatatt
     5341 aataaaatag aaaaagagcg aatttttaga gaaaccatta ttcaacgcta aatgaaaatc
     5401 gagtagaaat aatctactcg attttttgct ttgtaaattg acttcctgtc agttgatcaa
     5461 aaatatagtc ttttacttgt actgtcattg gatcatgtcc atagtctggt aataaaagat
     5521 gctttttagt tgatgtcaaa cggttaaaaa tggcaaattg acttgatgga ggacaaatat
     5581 catctttcaa ggctgttagt aaaacaacgg ggcaagaaat cctatgtgca aaatttttaa
     5641 catcaatata agccaaagtt tttaacacat tattttctgt tttatgaaaa ggatcactat
     5701 atttaaaata gcggaagagt tcgtcataag gctcactgac attccctaaa tctaatactc
     5761 gtctaaagtc tgacaaaaat gggtaaacag caacggtctt gacgattttg ggattaagag
     5821 ctgcagctat caaagcaagt gctccacctt gagaccagcc ataactataa agttgattac
     5881 tatcaacact ctctaatgta gcaatgatat caatcaattg gaaaacatca agataaatgt
     5941 ctttataaaa aagatgattt ggtcctgaga tcattcctcg cacaatctgg cccttaactg
     6001 taatgccatc aaaatgcccc ttgtcttgag actgtccagc ttgacctcga acatccattg
     6061 aaacaacgcc gtaacctgct gctacataat taagttggtc actccagtca ggactttggt
     6121 tttggtaacc atgaaaataa aaaactacag gatagggttt atttgtttta gggaataaac
     6181 atttggcata gacaatagag ttattgctgc ctttaaaatg aagatcataa gcttgtgcaa
     6241 aagtaatatt gaaatctttt ttgtctagac gatactcaat attcccttga tatttcattg
     6301 tttgtttttt ccagaagtca tcaaaatcct cgggaatttg gtcttgacct aggtattctc
     6361 tcatgtcatc tagcgacatt gtttcaatca tatacccgtc accccctgtt tttaaaatgg
     6421 tagtaagctc ctagcatacc agcttggttt tcgtgttgtg caaaaactat tattgttttt
     6481 tcagcaagac ttgtcacaag atttcgtttc aaagattcac taagtttatc ttgtaaataa
     6541 tctttttgag ccatgattcc accacctaaa acaacttttt caggatttac cacataaacc
     6601 atattagcga ttccttgacc aaggtagttt atcatacggt ctatacttgc aatacatttc
     6661 tcattgcctt ctttagcctc ctgaaaaata cgacgtccat cccatcggtt aatttcatct
     6721 ccatgcgctt tggcaacatc agcaatcagt gcagttgtag aggctaaatc ttgaaaatca
     6781 ccatctgaaa ggtgcatata gccaacttca caagcagaat tactaaagcc atgaaaaact
     6841 gttttatcaa taatcagaca accaccaata cctgttccaa tagtaagaca aagcgctatt
     6901 gaactatctt tggcactacc tgagacagct tccgctaatc ctgcacaatt aacatcattt
     6961 tcaatttctg tgcgtacttg ataagtgtct tctaaaactt tcttaaattg tgtcccagca
     7021 tagtttggaa tttgaggacc agagtaaaat atgcatcctt catctggatc taccatgcca
     7081 gcagatgaga tagcaattcc atcaatgctc ccttctgtta gatagttatc gataatttga
     7141 caaacttttt gtaaaatgcc tggaccacct ttgtaggctt ctgtggcaag ctcacttgcc
     7201 tcaacaatac aacctaaatt atccacaatg ccatgcttaa tcattgtacc gccaatatca
     7261 attgctactg ttctggtcat aattatctac ctcactttaa caaaactgtt ttttagcatg
     7321 tctaatcatt tctgctgcct ctttaataat aggtttatct tcttctgtta cagatgctaa
     7381 aggtaaccga acggaaccta tagttagttg ctcattgatt tccaatacag ctttgatcac
     7441 agcatacata ttaccatgac ccgaacaaag ctttgtgata atatcgttaa ttgtaaattg
     7501 aagttcacgc gctttttcta aatctttatc aacaattaac tgatttaaag tcaagtataa
     7561 ttctggcatt acgccatagg taccaccaat acctgcagct gctcccatga gacgaccacc
     7621 caaaaattgt tcatctggac cattaaaaac aatatgattt tctcctccaa tagccacaaa
     7681 attctgaatg tcttgaactg gcatggaaga atttttaaca ccaattactt gcggattttg
     7741 taacattttt cgatataaat ccgatgtcaa agcaacccct gccaattgag gaatattgta
     7801 aataataaaa tcagtttgtg gtgctgcttg actaatagta ttccaataat ctgctatggc
     7861 atactctggt aatcgaaaat aaattggtgg aattgctgca atagcatcaa cacccatagc
     7921 ttcagcatgc atagccaact cgacgctatc cttagtatta ttacatgcta catgcgctat
     7981 cactgttagt ttacccttag caacggacat aacattttcc agtacaagtt tacgatctgc
     8041 tacactttgg taaatacatt ctcctgatga accattaaca taaagtcctt gaacaccttt
     8101 atcaataaaa taattggtca gggcttttac tcgttcggga caaatatcac ccttatcatc
     8161 atagcaagca taaaatgcag gaataattcc ttgatatttt tgtaaatctt tcattgataa
     8221 tccccttgtt gtttgctgaa ataggcaaat tgtttttgta aaataataat tttaaagtaa
     8281 gcaaaggcgg tataagcaaa aataaatata gctgacactg taagcataaa taagagacta
     8341 gactgcacca taataaacca agtcatgatt aataaaaaca tcaatacacc agtccatttc
     8401 aagtttaaac atgccattat aaagctcttt ttcacggtat ctaaaagaga catttcatag
     8461 cgggcagcca taggaaatag gtatatgctc gtcatcacag tcaataacaa aaaggcgtaa
     8521 caaattatct tcaagaaagg cacaatgaaa cctatttgat aatgaagaat tgttaaatca
     8581 aatatgatca caaccaaaat acttagttca accaaaccta aaagcatacc ttggaaccac
     8641 tcttttttta ggtgttttat atacaatatt aaaagatttg tctgatcacc ttctaatttt
     8701 tgccacaacg ttcgatacaa actaatttta gcagcaccaa tagttaccaa tggtaggcaa
     8761 gttatcataa agagaaggtt tagcaatgcc agatcaaaaa caacattgca accttgcata
     8821 aaggggttgt taagatcaaa aatcgctgct attaattgat ttgctttttt cacaatatac
     8881 tccctgtatt tcttacgcca ttaacacttt caatacccct tttttgacat aattacctag
     8941 acttcccata ccatttggct gatgagcttc ttctgggaaa aagaaggcta tataattatc
     9001 tctcaaatca aaagaagtct cttctgagca cttgacaaat cctatatcgc ctaagtattc
     9061 ctctacctct accttatcac cttttcccaa tttgataact tcatatcctt caatgacata
     9121 atgtatatca aggtattttt tatggtattc aaaaatatga tcaaatgact cactaagttg
     9181 atttgattgg accatgagta tcacttctgg gctaatatga taggtgccta tatctaaatt
     9241 ccttaaatca tgacttagta gataatcaat cgctaaatct aaattaggat taatatcttt
     9301 ataatgcgtt aaattcaata aatgatcgta aatcataata aatcctacct atcctttaac
     9361 agctcccatt gtaatcccct gagtaaagga tttttggaaa actaagaata ctgtcacaat
     9421 aggaacagca gctaaggctg cacctgccat aattaagcca tagtttgttg ccatttcagc
     9481 ctgcatagta gctaccccaa gagaaatggt cagattattt ctagaagtta gcataactaa
     9541 ttgcataaaa tagtcattcc aagtattgat aaatgtgaaa atagctaggg cagcaaatcc
     9601 aggtttgaca attggaaatg ctacattaat aaatgttcga atctctccac aaccatcaat
     9661 tttagctgat tccagtaatt ctgttgaaat attttcagaa aactgtttca tcaagaagac
     9721 cccaaaaggc caaccaacaa gaggtaaaat aaccgcccat aaggtatcat gtatccccat
     9781 aaaattgata attctaacta atggcactaa aacaacttgt ttaggtaaag ccatagcagc
     9841 aataaaaagg gagaacaata ttttttgacc gtaaaatcgt ttcttagcta aaacatagcc
     9901 tgccattgac gatgtacagc aaactaggaa cattgtcata attgagataa agacactatt
     9961 ccaaagccat ctcaaagctg ggttttgtac agttaaagct ttaaaatttt ctaaggtcgg
    10021 tgctttgggc caccattgtg gggggatgat aatagtatca ggttgagatt taaaagctcc
    10081 tgtcataatc caataaaatg gaaagataaa aagcactgtc aaaagacaaa ggataagagc
    10141 cgttaaaaag ttataagcac tgaatgtttt ctttttcatg ttttcctcct aatattccac
    10201 atcatttcct aagatcttaa attgagcaaa acttattaaa gcaatcatta ctgctaggaa
    10261 tactcccata gtattagcat agccatattc tgatagttta aaggcttttt cataaagata
    10321 atacatcaaa gtgcttgttg aataatttgg tccaccagat gttaggagct ggattaaggc
    10381 aaaacattga aatgaattaa ttgttgttat aactgcaata tagagtgtag ttggtaagag
    10441 actcggccat ttaatttgcc aaaaaacttg catttcattg gctccatcaa cacgcgcagc
    10501 ttcgcaaaga gaattatcaa tatttcccat cgccgcaata tatagaataa taggttgacc
    10561 aactgaggtc gttagcaaaa taataataat agcaagtaat gcccagtgct tatcaccaag
    10621 ccagctaata ttctgttcaa tgacatgacc tgatttcaag atgtaattta aaattccgga
    10681 catagggtca taaatccatt tccaaacaac tgtgactgca acactaccag ttactactgg
    10741 caagaagaag acacaacggt agaaagagcg tgaaaaaaca tttttttcat aagtattggc
    10801 agcaacaaaa agtgagaaga aaaccactac tggcacagag ccaatcacaa tgattactgt
    10861 atttatcaag gatttcatga aaatagaatc atgaaacata cgattgtaat tagctaaccc
    10921 aataaaagtg aactgtgtca tagaataatt aaaaaaacta gtgacaaagc ccataaccat
    10981 cggtgcaaaa acaaaaatta agaaaaataa gagaataggc gctagaaaag cataagctat
    11041 catcgtttcc ctcatcttta acttattagt acgcatacgg tactctcctt tacatcttaa
    11101 cttgactaaa aactcctctt ctatcccaat atgtgattga ccatagaaaa ggagtctcct
    11161 taattattta gctgcttttt taatggtatc atttgctttt tgagtaaagt ctttcaaagc
    11221 atctgctggt ttttcatcac cattggatac agattgaacc attgggaacc ataaggttct
    11281 catttcagaa aatccatcga tagtgttgta atatggtgaa taatattgag tccattttga
    11341 aatcttcatc atacgtttat cacctttata aagatcccca aatgatgttc taactgggaa
    11401 agcacctgta cgtataacat cttttggtcc ccatttttta tcatcagcaa taaaagtgat
    11461 aaatttctta gaggctttta ctttgttttc atctttatta ttaaagaccg caaaaccatt
    11521 aacaaggtat tctaaatctg gttttccatc ttctgatggg aatggcactt caaggtaatc
    11581 cactttactt gactctaata attttgcttg agtttttggt tgagctggag cccataggat
    11641 agtgaaagca gtttgtccat tggcgaagtt ttgaatgtca gctgagccat catactgaga
    11701 cccattcatc aagtagcctt tcttaatcca ttcaactatt tttttcattg attttacaga
    11761 atttttagtg tcagtggtat attttgttac ttctttatct gttattggag cactataaag
    11821 attagcaaaa aatgcacgtg gtccttgatc tcctccttgc ccgtttgcaa agaatgaacc
    11881 tggtgtatag cctttatttt ttagtgcttt tagtactttt tcaaaatcac tagtagtcca
    11941 accttctttt acaagtttca aaactcctgc atctttaagc atttttttat tgaacgccat
    12001 ataaaatggg gcagaactta ttggatacat gtaggcttta tcgccagact tagaagcttg
    12061 aatgatgttc ttattattga catccttaat aaattggtct gtaaataaat cattcaaatc
    12121 tgctaattta ccatttttac catattgaat aattcgccct ggtgcatcaa aaagcacatc
    12181 aggtgctgtc cctgcttcaa ttgctgtagt gattttttca gatccagatg tgaaatcaat
    12241 tgtctctagt tttactttta tattaggatt tttcttttca aaagccttga tgactttttt
    12301 ctcataagta cctactccat ccttagcctt ttcttgagta aaggttggaa aagcccacca
    12361 cgtaatttcg actttatcct tctcggcagc cataacagat tgactactgt ctacacaagc
    12421 aaacattgat aatgctgcag ctccgaggca aaaaccaatc acactttttt tgatactcat
    12481 aactattccc cttttttatt tttattttga tttttgagta acacttttaa ctaagtcctg
    12541 agatgaaacg ctccgctatt tcttttggtc tagtgatagc acccccaact acaattcctg
    12601 caacacctat atgattaatt tcattagcct gcttaggagt atgaatttta ccttccgcaa
    12661 tcacatctat accggcttga caaagcttat taaggagttc tatatccggt ccttcttctt
    12721 ggcggctgta atctgtgtat cccgatagag ttgtacccac aaaatcaact cctgcttcaa
    12781 aagcattttt gccttcttca aaagtactta tatcagccat taacaactgt ttaggatatt
    12841 tcctttttat cttttgaata aactcagcta cactcaaacc atcatgacgc tctctaagtg
    12901 tacaatctaa ggctattact gcaatatcta aagtagctaa ttgatccacc tctgtcatcg
    12961 tagcagtgat aaatggttct tgtggaggat attcacgttt aataatgccg atgataggta
    13021 aattagtaac ttcttgaatt tccttaatgt cgcggacact attggctctt ataccaaccg
    13081 ctcctgcttc ttgagctgcc aaagctaaaa gaggcataac acctccactt tcagtataaa
    13141 gaggctcccc aggcaaagct tgacatgaca caataatgcc attttttatt tgctttttaa
    13201 aagcttcttt acttagatgt ggcatattct cacttcctta tcttttttaa ttttagatat
    13261 ctacaaacct tattatatct atttttaaag cgttttcaat aatctttaca gacttagatt
    13321 ttagttttaa cgttttgatt tagtcacggt taacgtgaaa gtagtttcag acttatagct
    13381 taatctcctc aatttaataa aaattatata agaaccttga aagtctattc aaggcatata
    13441 aaaaagaagc caaatagctt ccttttttat attatttgtt aaatgatacg tgaacgtggt
    13501 catagtggtt ggcagtaacg ccaccacgat ctggcattgc attccaagta ttagcaggtc
    13561 cataaatact atttgtattt gaataaaact tttgttgcca gataacatat gaaatgttat
    13621 ttgctgccat attttgtgta gagtactgtg caacttcatt accaagtgct tggtttttac
    13681 ctacaataaa gtcaactgct aaacctttac catgatcacc tggatctccc gcacggtatg
    13741 tactgaattc attaactcca taagttgacg ctactttttc tttataagct gcaacatgag
    13801 gttggagccc tgcattttca ggatgtgcag ctactgcatt tgttgttgaa gctggttgtg
    13861 ctaccggtgt tgctgttgga gctttttgtg ctaccggaac gctcttaact tcagtcgctt
    13921 gtaacttact gtctgtagct ggtgaagtcg tagtcacagg aactgctgga gctgatacat
    13981 gctctggtga tgcaccagtt tctactttag gagtgactac tttagcactt gccactctag
    14041 gggctgctac agttcttacc ggtgctactt tagctactgg agctggtgtt tcagcggcaa
    14101 cagaagctgg tgatactgtt gttaactgac tgactgacgt ctgagttggt ttaacttcct
    14161 ctttagctgc tggaacttct gaagtaatcg acttcacagg agctggtgat acctgttcat
    14221 tagctgctac ttgactaaca gcttgctctt gtgctaatac ttcttttgat ttcaaagctg
    14281 gcgcagaaga atatgtcttc attggcgaaa caatcgttgt tgctgcttct ggtgtcatac
    14341 cttccgaaat tgtattgaga gaaacttttt ggtctgcaac aaaaacttga ttggttttca
    14401 aatcgacagt agctgttgtt tgaccagcag catttgttgc tggtgtttct attttcattg
    14461 aagtggcagt atgactcttc tgatcgtaag ttactgtcag tgttgtctca ggataaataa
    14521 gattgatatc tgcaatgtta ttaatttttg ctaagacatt catatcaatt gacattgctt
    14581 ctgaaataac gcttagtgta tcaccatatt tcacagtata tgatgattta ttgtcttgct
    14641 ttaccaaatc agcctttacc tctgaaacag tacgtgctgt ccacgtcgta tctgtttctt
    14701 gtgcttgaac acttgcgact gataatagcg aagctgccat tgtcgatgtc aatagtacct
    14761 ttttattcat tttcattgct tcaattccta tttccttttt attagtccga gactataata
    14821 tcacacttta ctggactata aaattatttt cagacggttt aaagaaattg tacttagtta
    14881 gtaacaaaac tgtcatttaa aacaatgaat caatacctca aactatttgt aaagcagttc
    14941 ttgactgttg tcaacgcgaa gccaaactga actaccatta gcaaagcgtg tcttagccca
    15001 agttaaacca gctgttcctt ggatgggttt ttctacagtc ttaagagttt taggattaaa
    15061 gataaagtaa ccaccttttt gaagaacctg gtcagctgtc aaattaccat tagcatcaac
    15121 ctcatcaatt tctgatgctg gtataccatt atcgttccaa tcaaacccag tcggcgttag
    15181 ggtgttattt ttgactaacc aaacaccatt aaccttttgt aattcatcta ctcgatacac
    15241 ttttaattta ctagattgat tctgtacagg agctgattgt aatggcttaa tgcttggagc
    15301 tgatgcttgc gtttttccac taaaggtcgc aacgttagca attagtgacg ttggattgat
    15361 acgtccatgg aaaccatttt gaaaattagg gttagctggt aaaaattcaa aatgaaggtg
    15421 aggtcccgtc gccgtaccag ttgctcctac gtaaccgatg atatctcctt gtttgacttt
    15481 ttccccagtc ctagccacca cacgtgacat atgagcgtaa ccactatgca ttccatccgc
    15541 atgttgaatc atgacacaat ttcctgctaa gtctgtcatc caagaaaagt tggctccagc
    15601 tcctgcaaat ttcacagtac catctgccac tgccctaata atcgttccag tcggaacagc
    15661 ataatccacc ccacaatgtc caggataacc attgaaacct gttgtaattc taccattatc
    15721 aattggacgg acataagtat ccgctaaaac tcgggaaccc gcagataaaa ccataccacc
    15781 taaaacaact aaggaacttg ccttaactaa ccatttattc aagaaaaacc tccaaataga
    15841 attaatataa tgaagtgctc aaacacttgc aattaactta ttcgaaaatt taaatcaaaa
    15901 tatcaaaaaa agtactcacc ttgatgagta cttaggaggt ttaataaaaa gaaaagtttt
    15961 taggatatac gtagtatacc tgagttaact taagaaaacc taaactacta ttttttaatg
    16021 tctaaaatgt ctcacgcctg tgaagatcat ggtcaagcca tgtttgtttg cggcgtcaat
    16081 agattcttga tcacgaactg aaccacctgg ctggatgatt gctttgatgc ctgcggcagc
    16141 tatttcttca atgttgtccg caaatgggaa gaaggcatct gatgctagaa cggcaccgtc
    16201 aaggtggtcc ttagcctgct cgatagcaat cttgactgag ccgacacggt tggtttgacc
    16261 tacacccagt ccaagcgtca tgtgatcgtt agtaataata atcccgttag acttaacata
    16321 cttgatagcc ttccaggcaa actcaagggc agtcacctct tgttctgttg gctggcggtc
    16381 tgtcaccact tgccagtcag atggattttc agccacaacg tcttggtttt gcaccaaaag
    16441 tccaccaact acgccagtgt actcagcttc cacttcgctg gcagcttggg catcaaacgg
    16501 caactcaagg atacgcaagt ttttcttttt atttgtgaga atagctagcg cttcttctga
    16561 gtatgatggt gcgatgatga tttccaagaa gatagggtgc atcttctcag ctgtcgctgc
    16621 gtcaacttca cggttaagga caacaattcc accaaagatt gaaactggat cagcttcata
    16681 agcgtaatcc caagctgtct caatatcatc agcctgtccg ataccacatg ggttcatgtg
    16741 tttgagggca acaaccgttg gactgtcttt gaaatcgcgg ataatacgga ttgctgcatc
    16801 agcatcacgg atattattga aggacaattc ttttccattg agctgtttag ctgaagcgat
    16861 tgagtagtct gttggtaagg ctttttggta gaaatcagcg tcttgctgtg gattttctcc
    16921 gtaacgcata gcctgtttaa gatcataagt gatggtcaat ttttcaggct tagcctctcc
    16981 cacttgagct gtgaagtact cagcaatcaa agcgtcgtag gctgccgtat gacggaaggc
    17041 cttagctgcc aagcgttgac gagttttaaa tgtcgtctga ctagcgtcag ccaattctcc
    17101 caaaacagtg gcatagtcag ctggatcaac cacaacggtt acgctagcgt ggtttttagc
    17161 agctgagcga agcattgatg gaccgccgat gtcgatgttt tccaccgcca aatcgttggt
    17221 cacgtctggg cgaaggatag tctccttgaa gggatagagg ttgacaacca cgaggtcaat
    17281 caactcgatg ttgttgtcct tagcagcctg aaggtggctg tcagcgtcgc gacgagccaa
    17341 aatcccaccg tgaatgtttg agtggagggt cttaacacga ccatccatca tttctgggga
    17401 gccagttaca tcgtcaatag caatggtctc aacaccagca tcgtcaaggg caaccttagt
    17461 cccaccagtt gagataatat cccaacccaa gtttttcaat tcttttgcaa agtcaataat
    17521 tcctgatttg tcagaaactg agattaaagc acgtttagtc atgagttctc cttttcttac
    17581 ttcaatctat ttcataggct atgtattcat gagaaaattc ataaccgagt ttttcagata
    17641 aattcaatga agtccttgta tgagcatccc agctaggata aattccctta tctaaacaag
    17701 ttagtatcaa ctgagctgca actattgttg ctaaaccacg ccgacgaaaa tctggatgcg
    17761 tatctacttc tatctcgatc ccatttttat aggttgaata agatgaagct cctgcaatga
    17821 tattcccctg ataatatata acataaccta taccttgttt tttatagtac tgataagtag
    17881 cgtaatttgc tactaaatcc tgtgaccatt ccttttctaa gcaagagttg taaactttct
    17941 catcaataac acgtaattca aaaccatttg gcaactgagt aacaaacttc tctaacctac
    18001 ttcgctcaaa caaagtatct tttttcgtcg cataacgctt aaaagaatgg gcattctggc
    18061 cataagttga ttcaatcaaa tctgaccatc ctttatgctg aggaacaagg ataatatcct
    18121 ccccagagca aacttctagt agaaataaag taggctgacc tgctagaaag ccaaaagatg
    18181 atttccttcc caattttgcc aaagaagatt ttggttggtc taaactatct acaaaaactt
    18241 ctcccataat accttggaca cacgaccaga ttatggtctc gtcccaatca ccaaaaatag
    18301 actctactat ctctaaattt ttcactagtt tcattagctt attataacaa attttactat
    18361 tttctcttta tgcccaaact atccaaaaca tctggataga gttgatattc ggtttcatgg
    18421 atgcgggttt caaagctttc taggctatcg tctgctaggc gtggcacgcg cacttgttgg
    18481 atgacctgac cggtatccac accagagtcc acccaatgga tggtcacacc agactggtca
    18541 acacctgcct cccaagcatc cttgataccg tgagcacctg gaaattcagg cagataggct
    18601 gggtgaatat tgatgatacg cccttcatag gctgagagca aggtttctcc gacaatcttc
    18661 atgtagcccg ccagacagac caagtcaatc tcgtgtttgt ccaacaaatc aacgacggct
    18721 tgctcgtaag ccgccttatt ctcaaactct ttgagttcaa aagcgaagct tggaatggct
    18781 aagttctgag cacgctctaa aacataggca tcacgatgat ctgaaaagac aaaactaact
    18841 ggaaactgct ctgctatgac ctgaaagttg gaaccattag cagaagcaaa aacagcgatt
    18901 ttcatattcg atacaaaggg atgattcggc ttgccgaaaa atttctagaa aaataggaaa
    18961 acgaacaacg acgcttgcgt ctaagtcgtt ttatcttttt tcccagaaat ttaatcccgt
    19021 gttcagttag gcaaactaac taaacactcc tttcttataa aatttagtga cttatttaat
    19081 caccacacta tcgtctgctt tcttgatgat acgaccgatt tcataaactg gttcgtccaa
    19141 gagctctttg acgcggttaa cattttcagg actaacagct agcataagac cgacacccat
    19201 attgaagatt tcaaacattt cttcgtgctt gatgtcacca tatttttcaa gcgccttgaa
    19261 aatcggaagt actggcacct tatcctcatc gatttccgca gccaaatcat ccgcaaacat
    19321 acgaggaaca ttctcgataa agccaccacc cgtgatgtgg ccgataccgt tgactagttc
    19381 ttccttgatt aatggcagag ctgcttgaac atagatacga gttggctcaa gaaggacatc
    19441 cttgagttgt ttaccttcaa gctctggaag cagctcttta ccagtatagt cagcaaagac
    19501 acgacgcacc agcgaataac cgtttgagtg gataccactt gaagcaagtc ccaaaagaat
    19561 atcgccttcc tttactttgg aaccatcaag gagctgagat ttttcagcca caccaacagc
    19621 aaagccagca aggtcataat catcttcgcc atacatccca ggcatttcag cggtctcacc
    19681 tccaatgagt gctgctcctg cttgaacaca accttctgca acgccagaaa caacttcttc
    19741 aagtttgact gggttatttt ttcctgtcgc aatataatct aaaaaatata atggctcagc
    19801 acctgctgca ataatatcat tgacacacat ggcaacacaa tcttggccta tggtgtcatg
    19861 ctgttgatat ttaatcgcaa gcatgagttt tgtgccaaca ccgtctgttc ctgaaaccaa
    19921 aataggctct ctaactcctg ttttactcaa gtcaaacacc ccaccaaagc cacctagagc
    19981 tcccatgact cccgcacgtt ctgtacgaga aacgtgtttt tttattcttt caaccacttc
    20041 ataaccagct tcaacatcaa caccagattt tgcataagca tttttttcag acatgttttc
    20101 tccttatata attagcagag cttatttcac tttttgaata taaaaacttg tttcttcttc
    20161 taaacttctg aggtattctt cttcataatc ataaagcggt gttggataat gtccatcaaa
    20221 gtaagctacg cataaaccac catttggtgc ttttgtttcg agtccaattg attcaatcaa
    20281 cccatctagt gagagatagg tcagactatc tgcgccaata atatcacaca cttcgtcagc
    20341 agcgtgattt gctgaaatta attctcgtct tgtttgaata tcaataccat aaaaacaagg
    20401 atactttaat tctggactag ctatagcaac atgtacttca ctagctcctg cttctcttaa
    20461 taatccgaca atccttctag aagtcgttcc tcttacaatt gagtcatcaa tcataacaac
    20521 gcgctttcct ttgacaacac cagataccgc tgatagtttc attcgaacac cttgttccct
    20581 taattcttgt gtcggttgaa taaaggttcg ctgcgtatac tgatttttta caagacccat
    20641 ctcatttggt aatccggatt cttcagcaaa gcccatagca gccgataagg acgaatttgg
    20701 gacaccaatt acaatatcag catcctgttt aaattcttgt gcaagacgct ttcccatatt
    20761 ttttcgagcc gtatgaacat taacaccatg tatagttgaa tccggccttg caaaatagac
    20821 atattccatc gaacaaattg ccagttgcgt ttcatcagta taacgatcac attgaatacc
    20881 actatcgtct ataaggataa cttcgccagg ttcaacatct ctaacccatt ttgcgcctac
    20941 cacctcaaaa gcacaggtct cactggaaat aacccaagca ccattttgca tttgtccaat
    21001 tgacaaagga cgaaaggcat tagggtcaag agcagcaatt aatttatctt ctgtcatcag
    21061 tagataggcg aagcctccct ttacagtgct taaagcttct tttaccttcc ccataaaact
    21121 tgggttatgg cttcgacgaa tcaagtgcat caaaatttca gtatctgagg aggcattgaa
    21181 aattgcacct tgcttttcta attctttcct taaagaaata gcatttgtca aattaccatt
    21241 atggcataaa gcaaattgcc cgtcatgaaa tttataaaga aaaggctgaa tattgcgaat
    21301 atctgcagaa cctgcagtag cataccgaac atgtccaata gccgcattcc cagttaaatt
    21361 atctaattca gattgattct taaaaacttc agaaaggagc ccaacatttc gataaccata
    21421 gagtttccca ttatcattcg aaacaatacc agcaccttct tgaccgcgat gttgaaggct
    21481 atgaagccca aagtaagtga cttgagctgc ctgaggatgc ccccagatac caaagactcc
    21541 acattcttca tttagagatt ttacttcgta tgtcattctt ttttcctaat tctttttgta
    21601 tctcatctta agtagctttg atgattttct caattttgga tctatcgtca ccaatgccaa
    21661 catcaaaata gaaaatctat caagctattt tcaagctaat cattgagtct tagcctcaac
    21721 ggctcccaaa taccactacg acagaaagat acagttaagc tcgcttcgct cttgtttcct
    21781 tatctgtttt atgaaaaaat ttgaaaaaat tattttcctg taaaatattt tactgcacta
    21841 gcaaacaagg cttggtcttt gttaccaggg atattttgga agaggccgtc ttcccagcgt
    21901 tctgagtgcc ccatcttacc gatgatttga ccattcttgc tggtaatccc ttcgatggca
    21961 ttgacagagc catttggatt gtatttagaa tccatagatg gttgtccgtc aaagtccaca
    22021 tattggctcc agatttgacc attgtctctt agctctgcaa attcagaagc gctgacaaca
    22081 aatttacctt caccgtgtga aactgggatg gcatgaatat cgccgacctc aactcctacc
    22141 aaccaaggtg agttggtatt tgcgatacga gtctcaacca tcttggcaac gtgctggttg
    22201 gcatcgttat agaagagggt tggacttgtc tcaccagctt cctcgaagtt tccgtatgga
    22261 agaagacctg atttaacaag ggcttggaaa ccattacaga taccgatgat aaggccacct
    22321 ttttcgatga agctgtcaat agctgcgcga accttcttgt taagcaagat attgacgata
    22381 aacttagcag acccatctgg ttcatccgct gctgagaatc ctccagcaaa gaagatgata
    22441 tttgccttag caatattagc gaccattgtg tcaactgagt cagcaatagc agcctcattc
    22501 aaggttacaa atggtaccaa gttgacacta gctccaacct gttcaaaggc cttagctgaa
    22561 tcatattctg agttggtacc agggaagact ggaatgtaaa ccactggttt ttcaattgtt
    22621 tccttagcct tgataacagt atctgatacc acagcaggaa cttcttcaag agcgtctgcc
    22681 tgttcaaact ctgttgggta aacctcttcc aatttgcctt cgaagactga tagaaggctt
    22741 gccccagcaa ggtcatttcc attgacagta agtgtaaagg ctgcctgagt ttgaccgatt
    22801 ttcaccgagt cagcaatttc ctcagctgat gtaaagacaa agcctccgag ttgagctgtc
    22861 aagctactgt caagctctgc aatttcaaca gaggccccga tacggttacc aaaagtcatg
    22921 agagcaagac tttctaggac accaccgtat ttaacagctg aagcagcagt aatcttatgt
    22981 tgagcttgaa tagtctcgaa ctggctaaag ttagccttga taaggtcaaa atcaatatct
    23041 tctgaaatag cttgacctgg aatgtagtag atattctcac cagccgcttt aaactcagga
    23101 gagagaacct tgcggctgtc cgctgttgtc acaccgaaag ctaccaaggt tggcggtact
    23161 gtcaattctt cgaaagtacc agacatagag tccttaccgc cgattgatgg caatccaagt
    23221 tgaatctgag cctcaataga accaagaaga gctgatactg gctgatcaaa acgctctgcc
    23281 tgtttatcca tacgctcgaa atactcttgg taagagaaac gtgcacgaga ccagtcagca
    23341 cccgttgcta ccaagcgagc tgtcgcttca atgacagcat aggcagcacc gtggtaaggt
    23401 gaccattctg caatataagg attgtaacct tgagccataa cagatgcagt tgttgtgaca
    23461 ccgtgttgaa ctggcaattt ttgaacagag ctttctgttg gtgtgatttg gtaacggcca
    23521 ccgattgggt ggttaacggt tgagcgacca acagatgagt caaagatagt ttggagacct
    23581 ttttgactag cgtggttgag gtcagacaag accttaagcg tatccgcttc aagtgtctct
    23641 gctgatgttg tgcgtacctc tggaactgtc aagtctttgt caacgacttt agcatcaaca
    23701 acgacacgga caccgttggt atcaaggaaa cggcgttcca agtcaacaat tgtttcacca
    23761 tcccaagtca tgacaaggtt tggttttgca gtaatagtag caaccacaac ggcatcgata
    23821 ttttccttgt tgcaggctgc gatgaaggta tccacgtcac taggacgaac aacgactgac
    23881 atacgctctt gtgattctga gattgcaatt tcagtaccat taagaccttg gtatttaagc
    23941 ggtaccttgt ccaaatcgat ttcaagaccg tcagccaatt caccgatggc aacacagaca
    24001 ccacctgcac cgaagtcatt tgatttcttg attaggcgag tgacattacc atcacggaaa
    24061 aggcgttgaa tcttacgttc ttcgatggca ttcccttttt gtacctctgc gccagctgtt
    24121 tccacagatt caaccgtttg aaccttagat gaacctgtcg caccgccgac accgtcacga
    24181 cctgttttac caccgagcaa gatgaccaca tcgcctgctt ctggtttttc acgaaccaca
    24241 ttttccttag gtgcagcacc aaccacagct ccaagctcca tgcgtttggc tacaaagcca
    24301 gggtgaaagt actcacgcac ataagttgtc gcaagcccaa tctggttacc atatgaagaa
    24361 tagccgtgcg ccgcagtttt agaaataacc tgttgcggca atttaccagc acgtgtttcc
    24421 gcaatcggag tcgtaatatc gcctgcgcct gaaatacgca tagcctgata aacgtatgaa
    24481 cgtcctgaca acgggtcacg aatggcacca ccgatacaag ttgctgcgcc accaaatggc
    24541 tcaatttctg ttgggtgatt gtgagtctca ttcttaaaca tgaggagcca aggctctttt
    24601 acaccatcaa catctacttc aatttcaact gagcaggcat tgatttcatc tgagacttcc
    24661 atatcatcca gtcgtccatt ggcacgttca taacgaccaa agatagtcgc catatccata
    24721 agtgtctgcg gcttttcaga acgaccaagt tcatcacgca tggcgatata tttgtcataa
    24781 gtcgcctgca attgtttttg gaatttagaa gctgaaaagt cgatgttttt caactcagtt
    24841 tcaaaggttg tgtgacggca gtggtctgac cagtaagtat ccaaaacttt caactcagtt
    24901 tcagttggca cacgcccgat tgatttgaaa taatcttgga tgaagagaag atcatcgacc
    24961 tccatagcca agccctgctc tgccttatag gccgcaaaat cgtcagcctt ataagtttca
    25021 aagaaatcaa gactagggat tgtcttatca gacacagaga aagcctgctc ttcaagcggc
    25081 aaggtaatgt ccttgaaacg tgaatcaaca ggattcaaaa gatagttctt aacggcttca
    25141 agctctgctt ctgcaatatc cttattgacc aagtaaagct gggctgtatt gaccttaacc
    25201 tgactgtcac ttccaagcaa tagcaaagct tcttgcgaac tagcagcacg ttggtcaaat
    25261 tgaccaggaa gcgcctcaat ggcaaagaag gcaaccttat caagctccgc agtgatttca
    25321 gtttccgtca aaagacagtc tgtcacctgc tcagagaaaa tatgcttctc agcacgcgcc
    25381 agcaaatcct cagccaaatt gaagacatca tagacctgca caatacgcaa atccttcaaa
    25441 gaagtcagtt gtagattatg cgtcaactct ttcacaagac tagccgattt aatgtcaaag
    25501 tcagcctttt tctcaacaaa aatacgttta ttcatataag ataccaagtc cgacaaggga
    25561 gcgactgaaa aataggagga tatctgaaat tgagatttca gtaagcccaa tcttttttct
    25621 gagtttcccg aacggattca attccttcct cttctaatta caatgctatc aacttctcaa
    25681 gtacaacttg atagacatcg gtcaagctac caagatctct acgaaaaaca tccttgtcca
    25741 tatgatttcc ttcagcatcc catagacggc agttgtcagg tgagaactca tcagccaaga
    25801 tgattttgcc atccttgtca aatccgaact ccaacttgaa gtcaatcaag ttaagcccaa
    25861 tctgagcaaa ccaatctttc aagagttcat taatacggcg agtctctcct ttcagataag
    25921 caatttcttc atcattaaca atacccaaaa acttcatatg ctcatcattg ataaaaggat
    25981 cattcagatc atcatttttg tagtaaaatt ccacaattgg agtttcaaga acgcgtcctt
    26041 cttccactcc aaaacgtttt gaaaatgagc ctgctgtcac attccgtaat accacctcaa
    26101 gtggaataat atcaactttt ttattcaatt gttcgttttt tgaaatttgc tcaatataat
    26161 gtgtcaccac accagccata tttaatttct caaaaataag agatgaaatc tgattattta
    26221 gtgctccctt gccatcaata gtctccttac gagctccatt caacatagta gcttggtctt
    26281 tatagaccgt tctaatcaca ttttcatctt ttgtactata aatatctttc gcttttcctt
    26341 tataaataag ctggttagtc acttgattat tcgcctttct cttattttaa gagttattct
    26401 aacacacaac attatctaat acaacaaaaa ctttatttaa aatcaaaaaa aagaagaaac
    26461 gttcgtattt tcttcctttt tattattact tatgttcaat gatataatct aatagttgtt
    26521 ggacgcttga catgtgttcg acagcctcat ctggaatctc tagatgaaac acatcttcca
    26581 aattgataat aaactctgtt aaagcgatag agtcaattgc taaatcatca tgtaacgatg
    26641 actctggtgt gatatctaac tgtgggtcac caagttgctg ccttaataat tccagcatct
    26701 tttcaaaaac ttcatctctt gacatgagtt cactcctatt catctgtaaa ggcatcaact
    26761 aattgatcaa ctacttgagt ttccaacatt gtacgaactt gcttcaaagt agaataaaca
    26821 gctttagaat cacttgatcc atggcatttt actattggag cttttaaacc aaaaagaacc
    26881 gcacctcccg cgcttgaata gtccatacta tcttttagct gatacaggct atcttttaat
    26941 agaagtgctc ctaatttagc tttcacgcca cctgatttaa ttgaagattt tagacttccc
    27001 ataatattca tagctgtacc ttccattgtc tttagaacag catttccagt aaaaccatca
    27061 gtgacaacaa tatctgctac actagacatc aaatcacgtg cttcaatatt tccaataaaa
    27121 ttaatagaag gctctgctgc taacagttca taggcttctt tatgaagaga atcgcctttt
    27181 gtttcttccg ttccgttatt taagagacct acacgaggaa cttcaatacc acggacattt
    27241 tttgcataaa acgaccctaa aatagcatat tgatgaagat gagaagctgt gttttctgca
    27301 ttagctccta aatcaagcat atcaaaaccc acaccatcta acgtaggcat agttgacatt
    27361 aagcctggtc gatcaacacc ttttatacgt cctactacaa acaaaccagc agctaataaa
    27421 gctcctgtat tccctgcaga aataaaggcc tgtgcaacac catctttgac tgccttagct
    27481 cctaagacca tcgaagactg ttttttacga cgaacagctt tagcaggctc atcatcagaa
    27541 ttaatttttt cttcagtatg aataatacta actcgctccg ttactgttaa atatttctca
    27601 atttttttct gatcgccata gagttgtact tcaatatcac taaaatctga aatagcttga
    27661 ttaacaccct ctacaatagc tttgggagcg taatctccac ccatagcatc tacagcaatt
    27721 ttcttcatga atgaactcct cataaaaact tagtggctat attatagcat gtttctttat
    27781 ttttttcaca agaaggcaat aatagctaat atctaacaga tactttcact ctttccaaag
    27841 aaaagtatgc taaacaacct actaatatca aagaacatag tactccccct tcaaaaccaa
    27901 aatccccacc actccaaata gcaaagctag aattttcagt aattctaaaa agagaaacag
    27961 gagaataccc cccactaacc ttattaccta atacacatcc catagtaaaa ttccatgcag
    28021 tatgaactcc tgtaacaaaa agaatatttt tggtcatatt aaaaagaata gaaaaaataa
    28081 taccaagtaa aaataaatta aaaagtgcta ttgctgtgat accatagttt ctaaagtgga
    28141 taaaggaaaa caacatagaa ttgcataaaa cacctgcaaa actaccttta aatttggtaa
    28201 ctgttgtcat aacatatcct ctaaataaaa tttcctctgt caagctttgg ataaagtatg
    28261 caagtaataa aaagaaaaaa ggtccaaaag aaaaggaatt gctggaagaa atttccacaa
    28321 aacctgataa ccagtggcaa ata
//
LOCUS       DATROD010000015        27900 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.015, whole genome shotgun sequence.
ACCESSION   DATROD010000015 DATROD010000000
VERSION     DATROD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27900
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.015"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            22..471
                     /locus_tag="VBC74_000650"
     CDS             22..471
                     /locus_tag="VBC74_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEO6941621.1"
                     /translation="MNKNQYPKWEELPELDLYLDQVLLYVNQLINPKTITNDKLLTAS
                     MINNYVKHNYISKPIKKKYNRRQVARLIVITAFKQVFAIQEISQTLELLTADNHSEEA
                     YNGFAACMNKEEVHDLPPVVISACQTLNLYQETQKLVLELGGKNKNA"
     gene            464..1120
                     /locus_tag="VBC74_000651"
     CDS             464..1120
                     /locus_tag="VBC74_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEO6941622.1"
                     /translation="MLKEMSIKLSPQLSFGEELANSITHAVGALLMLILLPITAVYSH
                     NHFGLQAALGTSIFVTSLFLMFLSSSIYHSMTYNSLQKYVLRMIDHSMIYIAIAGSYT
                     PVALSLIGGWLGYLIIFLQWGITLFGILYKIFAPKINDKFSLVLYLIMGWLVIFIFPA
                     IITKTGPAFWGLLLAGGICYTIGALFYARKRPYDHMIWHLFILLASILQYIGIVYFML
                     "
     gene            1207..2121
                     /locus_tag="VBC74_000652"
     CDS             1207..2121
                     /locus_tag="VBC74_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEO6941623.1"
                     /translation="MKSAYIFFNPKSGKDEQALAKEVKSYLIEHDFQDDYVRIITPSS
                     VEEAVALAKKASEDHIDLVIPLGGDGTINKICGGVYAGGAYPTIGLVPAGTVNNFSKA
                     LNIPQERNLALENLLNGHVKSVDICKVNDDYMISSLTLGLLADIAANVTSEMKRKLGP
                     FAFLGDAYRILKRNRSYSITLAYDNNVRSLRTRLLLITMTNSIAGMPAFSPEATIDDG
                     LFRVYTMEHIHFFKLLLHLRQFRKGDFSQAKEIKHFHTNNLTISTFKRKKSAIPKVRI
                     DGDPGDQLPVKVEVIPKALKFIIPNSLP"
     gene            2222..3076
                     /locus_tag="VBC74_000653"
     CDS             2222..3076
                     /locus_tag="VBC74_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO6941624.1"
                     /translation="MTTFTAKFIDQEWEVPVESGRYHMIVGEFCPYAQRPQIARQLLG
                     LDKHISISFVDDVPSDIGLIFSQPEQVTGAKSLRDIYHLTDPTYQGPYTIPILIDKTD
                     NRIVCKESADLLRLFTTDFSDLHQEDAPVLFSQETASLIDNDIKDINKNFQSLMYKLA
                     FLDKQADYDTYSKEFFTFLDQKEHLLGQRPFLLGDNLSEVDIHFFTPLVRWDIAGRDL
                     LLLNQKALEDYPNIFSWAKTLYNDFNLKTLTNPQSIKNNYYLGKFGRAVRHHTIVPTG
                     PNMAKWEK"
     gene            complement(3122..3340)
                     /locus_tag="VBC74_000654"
     CDS             complement(3122..3340)
                     /locus_tag="VBC74_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEO6941625.1"
                     /translation="MIKLYYKDAYDTERCQVFENPNDFIRVLMGCVTVPDNYPITKMT
                     YKDKDIAYSGTYGDLFKTFIEFDWSTLD"
     gene            complement(3408..4403)
                     /locus_tag="VBC74_000655"
     CDS             complement(3408..4403)
                     /locus_tag="VBC74_000655"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEO6941626.1"
                     /translation="MTNRKDDHIKYALKYQSHYNSFDDIELIHSSLPKYNVNDIDLST
                     HFAGQSFEFPFYINAMTGGSEKGKAVNHKLAQVAQATGIVMVTGSYSAALKNDEDDSY
                     PTTDLYPDLKLATNIGLDKPVPAAESTVKAMNPIFLQVHVNVMQELLMPEGEREFHMW
                     RSHLKEYVDNIQCPLILKEVGFGMDLQSIKDAYDIGITTVDISGRGGTSFAYIENQRG
                     RDRSYLNTWGQTTAQSLINAQSMMDKMDILASGGIRHPLDMVKCLVLGAKAVGLSRTV
                     LELVERYPVDDVIAILNSWKEDLRMIMCALNCKKITDLRQVNYILYGQLKEANAK"
     gene            complement(4400..5392)
                     /locus_tag="VBC74_000656"
     CDS             complement(4400..5392)
                     /locus_tag="VBC74_000656"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEO6941627.1"
                     /translation="MVKVQTGGKLYIAGEYAILYPGQVAILKNVPIYMTALATFADNY
                     SLYSDMFNYTASLQPDKQYSLIQETILLMEEWLINFGKNIKPIHLEITGKLERYGLKF
                     GIGSSGSVVVLTIKAMAALYEIEMPSDLLFKLSAYVLLKRGDNGSMGDIACIAYEHLI
                     SYSAFDRRAVSKMIETKPLEQVLEAEWGYRITKIQALLEMDFLVGWTMQPSISKEMIN
                     IVKSTITQRFLDDTKYQVVQLLSAFKEGDKEAIKRCLEEISLLLFNLHPSIYTDKLQK
                     LKEASKGLDIVTKSSGSGGGDCGIAISFNKNDNQTLIKRWESAGIELLSKETLS"
     gene            complement(5385..6329)
                     /gene="mvaD"
                     /locus_tag="VBC74_000657"
     CDS             complement(5385..6329)
                     /gene="mvaD"
                     /locus_tag="VBC74_000657"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEO6941628.1"
                     /translation="MDGKSISVKSYANIAIIKYWGKADAEKMIPATSSISLTLENMYT
                     ETRLTALGKDAKKDEFYISGVLQNDHEHDKMSAILDRFRQNRSGFVKIETTNNMPTAA
                     GLSSSSSGLSALVKACNDFFGTNLSQSQLAQEAKFASGSSSRSFFGPVAAWDKDSGDI
                     YKVHTNLDLAMIMLVLNDKRKPISSREGMKICTETSTTFNEWVRQSEQDYQDMLVYLK
                     NNDFQKVGQLTERNALAMHSTTKTATPAFSYLTEETYKAMDVVKKLREKGHECYYTMD
                     AGPNVKVLCLRQDLEALAAILEKDYRIIVSTTKELADG"
     gene            complement(6311..7189)
                     /gene="mvk"
                     /locus_tag="VBC74_000658"
     CDS             complement(6311..7189)
                     /gene="mvk"
                     /locus_tag="VBC74_000658"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEO6941629.1"
                     /translation="MKEKFGIGKAHSKIILMGEHSVVYGYPAIAIPLKNIEVTCLIEE
                     APQLIALDMTDPLSTAIFAALDYLGKTSSKIAYHIESQVPERRGMGSSAAVAIAAIRA
                     VFDYFDEDLEADLLECLVNRAEMIAHSNPSGLDAKTCLSENTIKFIRNIGFSTVPMHL
                     NAYLVIADTGIHGHTKEAVDKVKSSGEAVLPFLKELGYLAEASEDAIHKSDSKQLGSL
                     MTKAHQSLKQLGVSSLEADHLVEVAISCGALGAKMSGGGLGGCIIALVKEKREAERLS
                     QQLEREGAVNTWTEKV"
     gene            complement(7305..8534)
                     /locus_tag="VBC74_000659"
     CDS             complement(7305..8534)
                     /locus_tag="VBC74_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6941630.1"
                     /translation="MFRNLRLRFIGIAALAILVVLFSVVGVLNSANHYQTKNEIYRVL
                     TILADNNGRIPNKLEFSKELGDDLSTDAIFQFRYFSARTDAKGNVTSFDSRNIFEVSD
                     RQIKNYAKRIVSQNSHSGHITYNFSTYSYLLKKVGKNDYLVVFLDTTNQYLDNQRLLQ
                     LSIWMSLVSFIVFMVIVSVLSGRVILPFVANYEKQRRFITNAGHELKTPLAIISANNE
                     LVEMMSGESEWTKSTNDQIQRLTGLINGMVSLARFEEQPDISMVDLDFSHITKDAAED
                     FKGPIIKDGKDFIMSIQPGIHVKAEEKSLFELVTLLVDNANKYCDPMGTVTVKLSRSS
                     RLRRAKLEVSNTYKNGKDIDYSKFFERFYREDESHNNKKSGYGIGLSIVTSLVHLFKG
                     SIDVNYKHDTITFVIYI"
     gene            complement(8534..9220)
                     /locus_tag="VBC74_000660"
     CDS             complement(8534..9220)
                     /locus_tag="VBC74_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6941631.1"
                     /translation="MRVLIAEDEEQMSRVLSTAISHQGYVVDVAYDGQTAIDLANQNA
                     YDVMVMDVMMPVKTGVEAVKEIRQSGNKSHIIMLTAMAEIDDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMSRRLEDFTPNVLSLGRVTLSVGEQELQCENTIRLAGKEAKMLAFF
                     MLNHDKELSTQQLFEHVWGADKDQEDVDEGYVFIYVSYLRQKLQAIGANLEIIGQEGS
                     SYKLSEINGG"
     gene            complement(9222..9848)
                     /locus_tag="VBC74_000661"
     CDS             complement(9222..9848)
                     /locus_tag="VBC74_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEO6941632.1"
                     /translation="METNIYGDYGRYLPLILEDFSQRIQLENDKAKVETGYKLYEHII
                     GRIKTSDSMIEKCRRKQLPVTVDSALKVIRDSIGVRIICGFVNDIYQIVERIKTFDDC
                     RIVVEKDYIQHVKPNGYRSYHVILEIDTPYPDCLGNSEGKYYMEIQLRTIAQDSWASL
                     EHQMKYKHDIENPERIVRELKRCADEIASVDLTMQTIRQLIESGTKKE"
     gene            complement(9956..11710)
                     /locus_tag="VBC74_000662"
     CDS             complement(9956..11710)
                     /locus_tag="VBC74_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6941633.1"
                     /translation="MKTKSQKTSFFSNLSPYLKGYKLLFALAIVLATLSSLTTVIGPD
                     RLKEMTDLMVKGLAGNIDLAKIGHIAGQLAVLYVLGALVSYLASFIVTTIIQKFAQGM
                     RNAIADKINKIPLSYFDSHTQGDTLSRVTNDVDLMTQSFNQSLVTMVASTVLLVGSLI
                     MMFKTDWHLATTAIISVFSGFALSSIIMVKSQPLFKKQQVNLANISGYVEEIYSGHNI
                     VTSHNARQQAKSDFQLLNDKLHSSMWKSQFFSGIMMPLMQFIGNFGYVMVCVVGAMLA
                     IKGTITMGTIVAFMTYVRIFTQPIGQIAQGITQLQSASAAMGRVFEFLNETNMQEDKH
                     KVLNLPKFKGHVSFEHVKFGYSDNKTIIHDFTSEAQPGQKVAIVGPTGAGKTTIVNLL
                     MRFYDVNSGSISIDDINIQDMKRSQVHDAFSMVLQDTWLFEGTIKENLIFNQTTISDD
                     EVQEAAKAVGIHHFIMTLPKGYDTVLDDAVTLSVGQKQLLTIARALLKNSPLLILDEA
                     TSSVDTRTEELIQKAMDKLMEGRTSFVIAHRLSTIKNADLILVMKDGNIIEQGNHDVL
                     MSQSGFYADLYNSQFESA"
     gene            complement(11700..13517)
                     /locus_tag="VBC74_000663"
     CDS             complement(11700..13517)
                     /locus_tag="VBC74_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6941634.1"
                     /translation="MFNIFKRLNGKEWLMIVASTILVCLAVWMDLKTPEYMSDITRML
                     QTKGTKLEALWDPGLKMLAFSFGSFLVAVMVGFLGARTAASFTSRLRSDIFNHVMDYS
                     DAEIKRFSIPSLLTRTTNDLTQLQILIVMGMQVVTRGPIMAIWALTKIGGKSDQWTGA
                     VGVAVIIVMILLSVLLLLAFPRQRQIQGFTDALNATTREALSGVRVIRAYNAEKYQTD
                     KFKRENDDLTKLNLFVYRLMSLMNPVMTVVSSGLTLAIYWIGSYLINDISVPKVTGTS
                     VSVLAAKSAITDRVDLFSNMVVFSSYAMQVVMGFMMMVAIFIIMPRALVSARRINEVL
                     TLAPTVTFKENSHIQSAPKGQIEFQDVSFHYSKDSEAVIEHVSFKADTGQTVAFIGST
                     GSGKSTLVNLVPRFYDATEGSIKLDGIDIKEYSHNDLNNKIGYIPQKAVLFSGTVRSN
                     MIFGESGREELSDEQIWQALELAQAKEFIEAKEKGLDTEVAQGGSNFSGGQKQRLAIA
                     RALARHPEILIFDDSFSALDYKTDHILRHVLKEKTAAMTKLIVAQRISTIMDADLILV
                     LDQGKVVGQGTHKELLSNNEVYQEIAYSQLSKEELENEN"
     gene            complement(13564..14004)
                     /locus_tag="VBC74_000664"
     CDS             complement(13564..14004)
                     /locus_tag="VBC74_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6941635.1"
                     /translation="MENPLQKARILVNQLEKYLDRYAKEYDVEHLAGPQGHLVMHLYK
                     HPDKDMSIKDAEEILHISKSVASNLVKRMEKNGFIAIVPSKTDKRVKYLYLTHLGKQK
                     ATQFEIFLEKLHSTMLAGITKEEIRTTKKVIRTLAKNMAMEDFD"
     gene            14273..16345
                     /locus_tag="VBC74_000665"
     CDS             14273..16345
                     /locus_tag="VBC74_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEO6941636.1"
                     /translation="MKKKIILKSSVLGLVAGTSIMFSSVFADQVGVQVIGVNDFHGAL
                     DNTGTANMPDGKVTNAGTAAQLDAYMDDAQKDFKQTNPNGESIRVQAGDMVGASPANS
                     GLLQDEPTVKTFNAMNVEYGTLGNHEFDEGLAEYNRIVTGKAPAPDSNINNITKSYPH
                     EAAKQEIVVANVIDKVNKQIPYNWKPYTIKNIPVNNKSVNVGFIGIVTKDIPNLVLRK
                     NYEQYEFLDEAETIVKYAKELQAKNVKAIVVLAHVPATSKDDIAEGEAAEMMKKVNQL
                     FPENSVDIVFAGHNHQYTNGLVGKTRIVQALSQGKAYADVRGVLDTDTQDFIETPSAK
                     VIAVAPGKKTGSADIQAIVDQANTIVKQVTEAKIGTAEVSGMITRSVDQDNVSPVGSL
                     ITEAQLAIARKSWPDIDFAMTNNGGIRADLLIKPDGTITWGAAQAVQPFGNILQVVEI
                     TGRDLYKALNEQYDQKQNFFLQIAGLRYTYTDNKEGGEETPFKVVKAYKSNGEEINPD
                     AKYKLVINDFLFGGGDGFASFRNAKLLGAINPDTEVFMAYITDLEKAGKKVSIPNNKP
                     KIYVTMKMVNETITQNDGTYSIIKKLYLDRQGNIVAQEIVSDTLNQTKSKSTKINPVT
                     TIHKKQLHQFTAINPMRNYGKPSNSTTVKSKQLPKTNSEYGQSFLMSVFGVGLIGIAL
                     NTKKKHMK"
     gene            complement(16382..16792)
                     /locus_tag="VBC74_000666"
     CDS             complement(16382..16792)
                     /locus_tag="VBC74_000666"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEO6941637.1"
                     /translation="MIKPIVRDTFFLQQKSQMASRADVSLAKDLQDTLHANQNYCVGM
                     AANMIGSLKRVIIINVGITNLVMFNPVLVAKSDPYETEESCLSLVGCRSTQRYHHITI
                     SYRDINWKEQQIKLTDFPAQICQHELDHLEGILI"
     gene            complement(16862..18211)
                     /gene="gdhA"
                     /locus_tag="VBC74_000667"
     CDS             complement(16862..18211)
                     /gene="gdhA"
                     /locus_tag="VBC74_000667"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEO6941638.1"
                     /translation="MTSGKTYVASVLEKVKKQNEHEEEFLQAVEEVFESLVPVFDKYP
                     QYIEENLLERLVEPERVISFRVPWVDDKGQVQVNRGYRVQFSSAIGPYKGGLRFHPTV
                     TQSIVKFLGFEQIFKNSLTGLPIGGGKGGSNFDPKGKSDNEVMRFTQSFMTELQKYIG
                     PDLDVPAGDIGVGGREIGYLYGQYKRLNGYQNGVLTGKGLTYGGSLARTEATGYGAVY
                     FAKEMLAARGQDLTGKVALVSGSGNVAIYATEKLQELGATVVAVSDSSGYVYDPDGID
                     LETLKQIKEVERARIVKYTEKHPKANFTPADQGSIWSIKADLAFPCATQNELDEEDAK
                     LLVENGVLAVTEGANMPSTLGAIKVFQKAGVAFGPAKAANAGGVAVSALEMAQNSSRR
                     AWTFEEVDQELQRIMKTIFVNASKAADEFGDSGNLVLGANIAGFLKVAQAMSAQGIV"
     gene            18379..18888
                     /locus_tag="VBC74_000668"
     CDS             18379..18888
                     /locus_tag="VBC74_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEO6941639.1"
                     /translation="MKWFPLVGGIIALLAGFYLFINPVTVVATLGWFIALFIFIYGIS
                     NLVRYLQADSYHRSPWYLVQSILAIIFGIILLSSSAMSLSNTVMTILAYWLLVSGILR
                     LLGGFQMQKINFPGAKSYKRSAYIALILGIILLVQPTLSAAIIGRFVAFILIAIGASA
                     IYTFTRINY"
     gene            complement(18929..20698)
                     /locus_tag="VBC74_000669"
     CDS             complement(18929..20698)
                     /locus_tag="VBC74_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6941640.1"
                     /translation="MRKKSIFLRLWSYLTRYKATLFLAIFLKVLSSFMSILEPFILGL
                     AITELTANLVDMAKGVSGAELNVPYIAGILIIYFFRGVFYELGSYGSNYFMTTVVQKS
                     IRDIRHDLNRKINKVPVSYFDKHQFGDMLGRFTSDVETVSNALQQSFLQIINAFLSII
                     LVVVMVLYLNVPLAMIIIACIPVTYFSAQAILKRSQPYFKEQAKILGELNGFVQEKLT
                     GFNIIKLYGREEASSQEFRDITDNLRHVGFKASFISGIMMPVLNSISDFIYLIIAFVG
                     GLQVIAGTLTIGNMQAFVQYVWQISQPVQTITQLAGVLQSAKSSLERIFEVLDEEEEA
                     NQVTEKLSHDLTGQVSFHGVDFHYSPDKPLIRDFNLDVEPGQMIAIVGPTGAGKTTLI
                     NLLMRFYDVSEGAITVDGHDIRHLSRQDFRQQFGMVLQDAWLYEGTIKENLRFGNLEA
                     SDEDIVAAAKAANVDHFIRTLPGGYNMVMNQESSNISLGQKQLLTIARALLADPKILI
                     LDEATSSVDTRLELLIQKAMKKLMEGRTSFVIAHRLSTIQEADNILVLKDGQIIEQGN
                     HQKLLADKGFYYELYNSQFSNSK"
     gene            complement(20703..22442)
                     /locus_tag="VBC74_000670"
     CDS             complement(20703..22442)
                     /locus_tag="VBC74_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6941641.1"
                     /translation="MAYIWSYLKRYPNWLWLDLLGAMLFVTVILGMPTALAGMIDNGV
                     TKGDRTGVYLWTFIMFIFVVLGIIGRITMTYASSRLTTTMIRDMRNDMYAKLQEYSHH
                     EYEQIGVSSLVTRMTSDTFVLMQFAEMSLRLGLVTPMVMIFSVVMILITSPSLAWLVA
                     VAMPLLVGVILYVAIKTKPLSERQQTMLDKINQYVRENLTGLRVVRAFARENFQSQKF
                     QVANQRYTDTSTGLFKLTGLTEPLFVQIIIAMIVAIVWFALDPLQRGAIKIGDLVAFI
                     EYSFHALFSFLLFANLFTMYPRMVVSSHRIREVMDMPISINPNTEGVTDTKLKGHLEF
                     DNVTFAYPGETESPVLHDISFKAKPGETIAFIGSTGSGKSSLVNLIPRFYDVTLGKIL
                     VDGVDVRDYNLKSLRQKIGFIPQKALLFTGTIGENLKYGKADATIDDLRQAVDISQAK
                     EFIESHQEAFETHLAEGGSNLSGGQKQRLSIARAVVKDPDLYIFDDSFSALDYKTDAT
                     LRARLKEVTGDSTVLIVAQRVGTIMDADQIIVLDEGEIVGRGTHAQLIENNAIYREIA
                     ESQLKNQNLSEGE"
     gene            complement(22509..22982)
                     /locus_tag="VBC74_000671"
                     /pseudo
     CDS             complement(22509..22982)
                     /locus_tag="VBC74_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(22979..24847)
                     /locus_tag="VBC74_000672"
     CDS             complement(22979..24847)
                     /locus_tag="VBC74_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO6941642.1"
                     /translation="MSDFLVDGLTKSVGDKTVFSNVSFIIHSLDRIGIIGVNGTGKTT
                     LLDVISGELGFDGDRSPFSSANDYKIAYLKQEPDFDDSQTILDTVLSSDLREMALIKE
                     YELLLNHYEESKQSRLEKVMAEMDSLDAWSIESEVKTVLSKLGITDLQLSVGELSGGL
                     RRRVQLAQVLLNDADLLLLDEPTNHLDIDTIAWLTNFLKNSKKTVLFITHDRYFLDNV
                     ATRIFELDKAQITEYQGNYQDYVRLRAEQDERDAASLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFQNLKNDLHQTSDTSDLEMTFETSRIGKKVINFENVSFSYPDKSILKD
                     FNLLIQNKDRIGIVGDNGVGKSTLLNLIVQDLQPDSGNVSIGETIRVGYFSQQLHNMD
                     GSKRVINYLQEVADEVKTSVGTTSVTELLEQFLFPRSTHGTQIAKLSGGEKKRLYLLK
                     ILIEKPNVLLLDEPTNDLDIATLTVLENFLQGFGGPVITVSHDRYFLDKVANKIIAFE
                     DNDIREFFGNYTDYLDEKAFNEQNNEVISKKESTKTSREKQSRKRMSYFEKQEWATIE
                     DDMMILENTITRIEDDMQTCGSDFTRLSDLQKELDAKNEALLEKYDRYEYLSGLDT"
     gene            complement(24858..26066)
                     /locus_tag="VBC74_000673"
     CDS             complement(24858..26066)
                     /locus_tag="VBC74_000673"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEO6941643.1"
                     /translation="MRLNYLPSEFQKALPILKKIKKAGYEAYFVGGSVRDVLLDRPIH
                     DVDIATSSYPEETKQIFKRTVDVGIEHGTVLVLEKGGEYEITTFRTEEVYVDYRRPSQ
                     VNFVRSLEEDLKRRDFTVNAFALNEDGEVIDLFHGLDDLDNHLLRAVGLASERFNEDA
                     LRIMRGLRFSASLNFDIETTTFEAMKKHAPLLEKISVERSFIEFDKLLLAPYWRKGML
                     ALIDSHAFNYLPCLKNRELQLSAFLSQLDKDFLFETSEQAWASLILSMEVEHTKTFLK
                     KWKTSTHFQKDVEHIVDVYRIREQMGLTKEHLYRYGKTIIKQAEGIRKARGLMVDFEK
                     IEQLDSELAIHDRHEIVVNGSTLIKKLGIKPGPQMGDIISQIELAIVLGQLINEEEAI
                     LHFVKQYLMD"
     gene            complement(26251..27099)
                     /locus_tag="VBC74_000674"
     CDS             complement(26251..27099)
                     /locus_tag="VBC74_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO6941644.1"
                     /translation="MKLALITDTSAYLPEAIENHEDVYVLDIPIIIDGKTYIEGQNLT
                     LDQYYDKLAASKELPKTSQPSLAELDDLLCQLEKEGYTHVLGLFIAAGISGFWQNIQF
                     LIEEHPNLTIAFPDTKITSAPQGNLVRNALMCSREGMDFDVIVNKIQSQIEKIEGFIV
                     VNDLNHLVKGGRLSNGSAIIGNLLSIKPVLHFNEEGKIVVYEKVRTEKKALKRLAEIV
                     KEMTADGEYDIAIIHSRAQDKAEQLYNLLAKAGLKDDLEIVSFGGVIATHLGEGAVAF
                     GITPKN"
     gene            complement(27099..27479)
                     /locus_tag="VBC74_000675"
     CDS             complement(27099..27479)
                     /locus_tag="VBC74_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEO6941645.1"
                     /translation="MEVIREQEFVNQYHYDARNLEWEEENGTPKTNFEVTFQLANRDE
                     AAKVTSIVAVLQFVIVRDEFVISGVISQIAHIQGRLINEPSEFSQDEVENLAAPLLEI
                     VKRLTYEVTEIALDRPGVTLEFNS"
     gene            complement(27551..>27900)
                     /locus_tag="VBC74_000676"
     CDS             complement(27551..>27900)
                     /locus_tag="VBC74_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941646.1"
                     /translation="TTETSFSKYFEYIYGINGKEHLEFLIKNGFVRMESPLDSLDHLS
                     APLLKLFLKEKNVKGLSKMKRSDLDQAIAIAFTEEELGKLFVVRGLALTEKGLAALSN
                     NQEVIDRHPKKKF"
BASE COUNT         8945 a         5578 c         4293 g         9084 t
ORIGIN      
        1 tagattatat ggagttttaa aatgaataaa aatcaatacc caaaatggga agaattacct
       61 gaactagatt tgtatcttga ccaagtactt ctctatgtga atcaattaat taatcctaaa
      121 acaataacta acgataaact actcaccgct tcgatgatca acaattatgt caaacataat
      181 tatatatcca aacctataaa aaaaaaatac aatcgccgtc aagtagcacg attgattgtt
      241 attacagctt ttaaacaagt ttttgctatt caagaaatca gtcaaacatt agaacttctg
      301 acagctgaca atcattctga agaagcatat aacggatttg ctgcttgtat gaacaaagaa
      361 gaggttcacg atttaccacc agttgtcatt agtgcctgtc agacgttgaa cctttatcaa
      421 gaaacacaaa aattagtttt agaactagga ggaaaaaata aaaatgctta aagaaatgtc
      481 tatcaaatta agtcctcaac tttcctttgg cgaagagctt gcaaatagta tcactcacgc
      541 tgtaggagct ttgcttatgc taatcctatt accaatcacc gctgtctata gtcacaacca
      601 cttcggatta caagcagcac ttggtacctc aatattcgtc acttctttat ttttgatgtt
      661 tttatcatca agtatttacc actccatgac ttataacagt cttcaaaaat atgttctacg
      721 catgattgat cactctatga tttatattgc tattgcaggt agttatactc ctgtcgcatt
      781 gtcacttatt ggcggctggt taggatatct catcattttt ttacaatggg ggattacatt
      841 atttggtatc ctttataaaa tcttcgctcc caaaatcaat gataaattta gccttgtact
      901 ttacctcatt atgggatggc tggttatctt tattttccct gctattatca caaaaactgg
      961 accagctttt tggggattac tcttggctgg tggtatttgc tatactatcg gtgcactctt
     1021 ttatgctcgc aaacgtcctt atgatcacat gatttggcac ctttttattc tattagcttc
     1081 tattttacaa tacattggaa ttgtctactt catgctataa aatttaacta cattttattg
     1141 attatttttt tgctaaacca tgatataata ataatcatct aaatcttaga gagaaggcgt
     1201 ggacatatga aaagtgctta tatctttttt aatccaaaat ctggtaaaga tgaacaagca
     1261 ttggcaaaag aagtaaaatc ttatttaata gaacatgatt ttcaagacga ttatgttcgt
     1321 attattacac cttcatctgt tgaggaagct gtcgccttag caaaaaaagc atctgaagac
     1381 catatcgact tggttattcc tttaggggga gatggcacta ttaataaaat ctgtggtggt
     1441 gtctacgctg gtggtgctta tcctactatc ggactagtac ctgccgggac cgtcaataac
     1501 ttttcaaaag ctttgaacat cccgcaagag aggaatctcg ctcttgaaaa ccttctaaac
     1561 ggtcatgtaa agtccgtaga tatctgtaaa gttaatgacg attacatgat tagcagttta
     1621 acacttggtc tccttgctga tattgctgct aatgtcactt ctgaaatgaa gcgtaaatta
     1681 ggcccttttg ctttcttagg tgatgcctac cgtatattaa agcgtaatcg atcatattct
     1741 atcactcttg cttacgataa taatgtacgg tctttacgca cgcggttact ccttattacc
     1801 atgaccaatt cgattgccgg tatgccagcc ttctctccag aagcaacaat tgatgatgga
     1861 ctttttagag tctacactat ggaacatatt cacttcttca aattattgtt gcatctaaga
     1921 caatttcgta agggagattt tagtcaagca aaggagataa aacattttca taccaataat
     1981 ctgactattt caacttttaa acgtaaaaaa tcagctatcc ctaaagttcg tattgatggc
     2041 gatccaggtg accaattacc tgtaaaagtt gaagtcatcc ctaaagcctt aaagtttatc
     2101 attcctaact cattaccata atataaaagg acctagacat aagtattaga gtaaatacta
     2161 tctgtctagg ttcttttttg ttatacttaa agcgataaat atcattgaaa ggaagtatac
     2221 gatgacaaca tttactgcta aatttattga ccaagagtgg gaagtcccag tagaatcagg
     2281 tcgctaccat atgattgttg gggaattttg tccttatgca caacgtccgc agattgcgcg
     2341 ccaactgctt ggtttggata aacatatttc tatcagtttt gtggatgatg ttccaagtga
     2401 tataggactt atttttagcc aaccagaaca ggtcactggt gctaagtcgc ttcgtgatat
     2461 ttatcatttg actgacccga cctaccaagg tccttatact atcccaattt taattgacaa
     2521 gaccgataat cgtattgtct gtaaggaatc tgcagatttg cttcgacttt ttactacaga
     2581 tttctctgat ttgcatcaag aggatgctcc tgtccttttc agccaagaaa ctgcttcact
     2641 tattgataat gacattaaag acattaataa gaacttccaa agcttaatgt ataagttagc
     2701 ttttcttgat aaacaagcag attatgatac ttatagtaag gaattcttca cttttctaga
     2761 tcaaaaagaa cacttactag gacaacgtcc tttcttgctc ggagataatc taagtgaggt
     2821 tgatattcat ttctttacac cattagtccg atgggatatt gctgggcgtg atcttctact
     2881 acttaatcag aaagcgctag aagactaccc aaatattttt tcttgggcta aaactttata
     2941 caatgacttt aatttaaaaa cattaaccaa tccacaatcc ataaaaaata actattactt
     3001 aggcaaattt ggaagagctg ttcgtcatca taccatcgtc ccaacaggtc caaatatggc
     3061 aaaatgggaa aaataaagct aaaaagctaa ggcaattcac agccttagct ttttagctat
     3121 actagtccaa agtggaccaa tcaaattcaa taaaagtctt gaagagatct ccataagtac
     3181 cactgtaagc aatatcttta tctttataag tcattttcgt aataggataa ttgtcaggaa
     3241 cagtaacaca tcccattaaa actcgaataa aatcatttgg attttcaaaa acttggcatc
     3301 gttcagtgtc gtaagcatct ttataataca gtttaatcat cttattacct ctgacgtttt
     3361 acaaggaaac tttgacagga agctatatct acttcacaaa aataatatca tttagcattg
     3421 gcttccttca attgaccata caatatataa ttgacttgtc gtaaatcagt tattttttta
     3481 caattcagtg cacacattat cattcttaaa tcttctttcc aagaattgag aattgcaata
     3541 acatcatcta caggataacg ctcaactaat tctaaaactg ttctagaaag tccaacagct
     3601 ttagcaccca acactaaaca ttttaccata tctaaaggat gacgaatgcc accagatgct
     3661 aggatatcca ttttgtccat catagattga gcgtttatta aggattgagc tgttgtttgc
     3721 ccccaggtat tgagatacga acggtcgcgt ccacgttgat tttcgatgta agcaaaacta
     3781 gtccccccac gccccgaaat atctacagtt gtgataccta tatcgtaagc atcctttatt
     3841 gattgtaaat ccatcccaaa accaacctct tttaaaataa gtgggcattg gatattgtcg
     3901 acgtattctt ttaagtgaga acgccacatg tgaaattcac gctctccttc aggcatcaaa
     3961 agttcttgca tcacattaac atgaacttgt aaaaaaatgg ggttcatagc cttaacggtt
     4021 gactctgctg ctggtacagg cttgtctaac cctatatttg tcgccagttt taaatcagga
     4081 tacaagtctg ttgttgggta ggaatcatct tcatcgtttt tgagagctgc cgaatatgaa
     4141 cctgtcacca taacaatccc tgtagcttga gcaacctgtg ccaatttatg attgactgct
     4201 ttcccttttt cactaccgcc tgtcatagca ttgatataaa aaggaaattc aaagctttga
     4261 ccagcaaaat gtgttgataa atcgatatcg ttgacattat attttggtaa ggatgaatgt
     4321 attaactcaa tatcgtcaaa actattataa tgcgactgat atttaagagc atattttatg
     4381 tgatcatctt ttcgatttgt catgacagtg tctccttact aagtagttca ataccagcac
     4441 tctcccaacg cttgattaga gtttgattat catttttatt aaaggatatg gctataccac
     4501 aatcgcctcc tccagatcct gaagattttg tgactatatc taggcctttg cttgcctctt
     4561 taagcttttg aagtttatcg gtgtaaatag atggatgaag attaaataat agcaagctaa
     4621 tttcttctaa acatctctta atcgcttctt tatctccttc tttaaaggct gaaagtaact
     4681 ggacaacttg atactttgta tcatctaaaa aacgctgcgt aatagttgat ttaactatat
     4741 ttatcatttc ttttgaaatg gacggttgca ttgtccatcc cactaaaaaa tccatctcca
     4801 ataaagcttg tattttggta atacgataac cccattcagc ttccaatact tgttccaaag
     4861 gttttgtttc aatcattttt gaaacagccc tacgatcaaa agctgaataa gaaattaaat
     4921 gctcataagc aatacaagca atatctccca ttgagccatt atcacctctt tttaagagaa
     4981 cataggcaga taatttaaaa agcaagtcgc ttggcatctc tatctcataa agcgcagcca
     5041 tagctttaat agtcaaaacg acaacacttc cgctagagcc tataccaaat ttaagtccat
     5101 agcgctctaa ttttcccgta atttcgaggt gaatcggttt tatatttttg ccaaaattaa
     5161 tcaaccattc ttccataagc aatatcgttt cctgaatgag actatattgc ttatctggct
     5221 gtaagcttgc cgtatagtta aacatatcag aataaaggct gtagttatct gcaaaagtag
     5281 ctaatgcagt catataaata ggaacgttct ttaaaatagc tacttgccca ggatacagta
     5341 tcgcgtattc acctgcaata tataatttgc cacctgtttg caccttaacc atctgctaat
     5401 tcctttgtcg ttgaaacaat aatacgataa tctttctcca agatagcagc taatgcctca
     5461 aggtcttgac gtaagcaaag tactttaaca tttggacccg catccatcgt ataataacat
     5521 tcatgacctt tttcgcgaag tttcttcaca acatccatag ctttatatgt ctcttctgtc
     5581 aaatatgaaa aggctggagt tgctgtttta gttgttgagt gcattgcaag tgcattacgc
     5641 tctgtcagtt gacctacctt ttgaaaatca ttatttttta ggtatactaa catatcttga
     5701 taatcttgtt cagattgcct tacccattca ttaaaagtag ttgaagtttc ggtgcagatt
     5761 ttcatacctt cacggcttga aatcggcttt cttttgtcat ttaagaccag cataatcata
     5821 gctaaatcta aattagtgtg aactttatag atatcaccac tatctttatc ccaagcagca
     5881 acaggtccaa aaaaagagcg tgaagaggag cctgaagcaa acttggcttc ttgagctagc
     5941 tgtgattggc taaggttagt accaaaaaaa tcattgcagg cctttactaa ggctgataaa
     6001 ccacttgatg acgatgataa gcctgcagct gttggcatat tattagttgt ttcaatcttt
     6061 acaaagccgg acctattttg tcgaaagcga tccaagatag ctgacatttt gtcatgttca
     6121 tgatcatttt gaagaacacc agagatgtaa aattcatctt tcttagcatc cttcccaagg
     6181 gcagtcaaac gtgtttcagt gtacatgttt tcaagtgtta gagaaatact actggtagct
     6241 ggaatcattt tttcagcatc tgcttttccc caatatttga taatagcaat gttagcataa
     6301 gacttaacac ttatactttt tccgtccatg tgttgacagc tccttctctt tctaattgct
     6361 gacttaagcg ctcagcttct cttttttctt ttacaagtgc gataatacaa ccacctaatc
     6421 cgccgccact cattttagcg cctagagcac cacaactaat agctacctct accagatgat
     6481 cagcttctaa acttgagaca cccaattgtt ttaaactttg atgtgcttta gtcatcaagc
     6541 taccaagttg tttggaatca cttttatgaa tagcatcctc tgatgcctct gctaaataac
     6601 ctaattcttt taaaaagggt agtactgcct cgccagatga tttcacctta tccactgctt
     6661 ctttagtatg cccatgaatt cctgtatcag caataactaa ataagcattc aaatgcatgg
     6721 gtactgttga aaaaccaata ttgcggataa atttaatagt attttcacta agacaagttt
     6781 ttgcatctaa tccactcgga tttgaatgtg ctatcatttc tgctcgatta acaaggcatt
     6841 ctaacaaatc tgcctccaaa tcttcatcaa aataatcaaa aactgcacga atagctgcaa
     6901 tagcaactgc agctgatgat cccataccac gtctttctgg gacttgtgac tcaatatgat
     6961 atgcaatttt agaagaagtt ttacctaaat agtccaaggc agcaaaaatg gctgtcgaca
     7021 aaggatcagt catatctaat gctattaatt gaggagcctc ttcaataagg caagttactt
     7081 caatattttt taaaggaata gcaatggctg gataaccata tactactgag tgctcaccca
     7141 tcaagataat cttgctatga gcctttccta taccaaactt ttctttcatc tttttctctt
     7201 tacaattcta ttaccttact attttaatat aaatccttaa aaaaagcgac tttaacgtga
     7261 taataacata aattaaagga actacaattg cagtcccttt aatcttaaat gtaaatgaca
     7321 aaggtaatgg tatcatgttt ataattcaca tcgatacttc ctttaaatag atgcaccaaa
     7381 ctagttacga tagaaagacc aataccatat cccgattttt tgttattatg tgactcatcc
     7441 tctcgataaa atcgttcaaa aaacttgcta tagtctatgt ctttaccatt cttataagta
     7501 tttgaaactt caagcttagc tcgtctcaaa cgacttgaac gtgataactt aactgtcaca
     7561 gtccccatag gatcacaata cttattagca ttatcaacca ataatgtaac caattcaaat
     7621 aatgattttt cttcagcttt gacatgaata cctggttgga tactcattat aaagtcttta
     7681 ccatctttaa tgattggacc tttaaaatct tctgcagcat cttttgtaat atgtgaaaag
     7741 tctaagtcaa ccatacttat atcaggctgt tcttcaaagc gggcaagact gaccatgcca
     7801 ttaattagac ccgttaaacg ttggatttga tcatttgtag atttagtcca ctctgattca
     7861 ccactcatca tttccaccaa ttcattatta gcggagatga tagcaagtgg agtttttagt
     7921 tcatgtcctg cattggtaat aaaacgcctt tgcttttcat agttagcaac aaatggtaag
     7981 ataactctcc ctgataaaac tgaaacaatt accatgaaga cgataaaact taccaaggac
     8041 atccatattg aaagttgcaa taatctttga ttatcaagat attggttggt tgtatctaaa
     8101 aataccacta aataatcatt tttaccaacc ttttttagta gataagaata agtactaaaa
     8161 ttataggtga tatgacctga atgactattt tgtgatacga tacgcttggc ataatttttt
     8221 atttgcctat cgcttacttc aaagatattt ctagaatcaa aactagtcac gttacctttt
     8281 gcatctgttc gcgcactgaa ataacggaat tggaaaatag cgtccgttga taggtcatca
     8341 cctaattctt tactaaattc aagtttattc ggtatacgtc cattgttgtc tgcaaggata
     8401 gttaacacac gataaatttc attcttcgtt tgatagtgat tagcagagtt gaggacaccg
     8461 actactgaaa ataagacaac aagaatagct agcgcagcta ttccgataaa acgaagtcgt
     8521 aaatttctaa acattagcca ccatttattt cacttaattt atagctacta ccttcttgac
     8581 caataatctc caagttagca ccaattgcct gcaatttttg tctcaaataa gaaacataga
     8641 taaagacgta tccctcatct acatcttctt ggtccttatc tgctccccag acatgttcga
     8701 aaagttgttg tgtagataat tctttatcat ggttcaacat gaaaaatgct aacattttag
     8761 cttctttacc cgctagacga atagtgtttt cacattgcaa ctcttgttca ccgactgata
     8821 aggttactct tcccaatgac aatacattag gtgtgaaatc ttccaatctt cgggacatag
     8881 agcgaagcct tgctaataat tcttttagag agaagggttt tgttaaataa tcatctgcac
     8941 cagcatctag acctgttact ctatcatcaa tttctgccat ggctgttagc ataataatgt
     9001 gagatttatt tcctgattgt ctaatttctt tgacagcctc aacacctgtt ttaacaggca
     9061 tcatgacatc cataaccatg acatcatatg cattttgatt agctaaatca atagcagtct
     9121 gaccatcgta ggcgacatca acaacataac cttgatgact aattgccgta ctaaggacac
     9181 gtgacatctg ttcttcatct tctgcaatta atactctcat tttattcctt ctttgttcca
     9241 ctctctatca attgacgaat cgtttgcatc gttaaatcaa cagatgctat ttcatcagca
     9301 caacgtttta attctcttac aattcgttcg ggattttcaa tatcatgctt gtatttcatc
     9361 tgatgttcta aactagccca agaatcttgc gcaattgtac gcaactgaat ctccatgtaa
     9421 tatttacctt ctgaattacc caaacagtcc ggatagggag tatcaatttc taaaatcaca
     9481 tggtaagagc gatacccatt tggcttaaca tgctggatat agtctttttc aactacaata
     9541 cgacaatcat caaatgtctt aatacgttct acaatttgat aaatgtcgtt aacaaaacca
     9601 caaataatac ggactccaat actatctcta ataactttta gtgcagaatc tactgttaca
     9661 ggtaactgtt tacgacgaca tttctctatc atactatctg atgtcttaat acgaccaatg
     9721 atatgttcgt acaatttata gcctgtttca accttagctt tatcattttc taattggata
     9781 cgctgactga aatcttctaa aattaatggt agatatcgac cataatctcc ataaatattt
     9841 gtttccataa gaaaccttcc tattctctct taagctccat tatatcatag aaacttaagg
     9901 caaactaaaa aggaatgaga attttttctt cccatttcct tctaaaatgt ttatgttaag
     9961 ctgattcgaa ttgagaatta tacagatcag cataaaaacc actttgagac atcaaaacat
    10021 catgattgcc ttgctcaatg atattcccat ctttcatgac caaaatcaag tctgcgtttt
    10081 taatcgttga taaacgatgt gcgataacaa atgatgttct tccttccata agtttgtcca
    10141 tagctttttg aattaactct tctgtacgtg tatcaacaga tgaagttgct tcatctaaga
    10201 taagtaaggg agaatttttc aaaagagctc gagcaatggt taataattgc ttttgtccaa
    10261 ctgacaatgt cacagcgtca tcaaggacag tatcataccc tttaggtagt gtcattataa
    10321 aatgatgtat accgactgcc ttagcagctt cttgaacctc atcatcactg attgtcgtct
    10381 ggttaaaaat caaattttct ttaatagttc cctcaaacaa ccatgtatct tgaagaacca
    10441 tcgagaaagc atcatgaact tgactacgtt tcatgtcttg gatattgatg tcatctatgg
    10501 aaatactacc tgaattaaca tcataaaaac gcatcaataa attaacaatt gtcgttttac
    10561 ctgcgccagt tggtcctaca atagcaactt tctgaccagg ctgtgcctcc gaagtaaagt
    10621 catgaataat agtcttgtta tcactataac caaacttgac atgttcaaag gacacatgcc
    10681 ccttaaactt aggaagattt aacactttgt gtttatcctc ttgcatattt gtttcattta
    10741 aaaattcaaa aacacgtccc atggctgcac tagcagactg taattgtgta attccttgag
    10801 caatctgacc aataggctgt gtaaaaatac gaacataggt cataaaagca acaattgtac
    10861 ccatagtaat tgttcctttg atagctaaca tggcaccaac cacacacacc atcacataac
    10921 caaaattacc aataaactgc atcagaggca tcataattcc agagaaaaat tgtgatttcc
    10981 acatggaact gtgaagttta tcatttaata gttgaaaatc tgacttagcc tgctgtcttg
    11041 cattatgact agtaacaatg ttgtgtcctg agtaaatttc ttctacatat ccagatatat
    11101 tagccaaatt cacctgttgc tttttaaata aaggctgact cttcaccata atgattgaag
    11161 aaagagcgaa tcctgaaaat accgatataa tcgccgttgt tgctaaatgc caatctgttt
    11221 tgaacatcat aattaatgaa ccaaccaata acactgtaga tgcaaccata gtaactaaac
    11281 tttggttaaa agactgtgtc atcaaatcca catcatttgt aacacgagat aaagtatcac
    11341 cttgagtatg gctatcaaaa taactcaaag gtattttatt gattttatca gcaatagcat
    11401 tgcgcatacc ttgagcaaat ttttgaataa tagtcgtcac aataaaactt gctagatagg
    11461 atactaatgc acctaaaaca tataatactg ccaattgccc tgcaatatga ccgattttgg
    11521 ctaagtcaat attaccagcc aagcctttta ccataagatc cgtcatctct ttaagacggt
    11581 ctggtcctat aactgttgtc aagcttgata aagttgctaa cacaattgct aacgcaaaaa
    11641 gaagtttgta acctttgagg tacggagata aattagagaa aaaagacgtt ttttgtgatt
    11701 tagttttcat tttccaattc ctccttagac aattgagaat aggctatctc ttgatatact
    11761 tcattatttg ataacaattc cttatgtgtt ccttgcccaa caactttacc ttgatctagc
    11821 actaaaatga gatcagcatc catgatagtt gaaatgcgtt gagcgacaat taatttcgtc
    11881 atagcagctg ttttttcttt cagcacatgg cgtaaaatat gatctgtttt ataatctaac
    11941 gctgaaaatg agtcatcaaa aatgagaatt tcagggtgac gtgctaaagc acgtgcgatt
    12001 gccaagcgtt gtttttgtcc tcctgaaaag ttactaccac cttgagcgac ttctgtatct
    12061 aatccttttt ctttagcttc tataaattct ttagcttgcg ctaattcgag tgcttgccaa
    12121 atttgttcat cactaagttc ctccctgcca ctctctccaa aaatcatatt agaacgtact
    12181 gtcccactaa agagaacagc tttctgtgga atatagccaa ttttattatt caaatcattg
    12241 tgagagtatt cctttatatc aatgccatca agtttaattg acccctctgt ggcgtcataa
    12301 aaacgtggaa ctaaattgac taaagtggat tttccagaac ctgtagaccc aataaatgcc
    12361 actgtctgac cagtatctgc tttgaaagat acatgttcaa taaccgcttc agaatcttta
    12421 ctataatgaa atgacacatc ttgaaactca atttgacctt ttggtgcact ttggatatga
    12481 ctattttctt taaaagttac tgttggggct aaagttagta cctcattaat acgtctagcc
    12541 gatactaaag cacgaggcat gatgataaaa atagctacca tcatcataaa tcccataaca
    12601 acttgcatag cataagatga aaaaacaacc atgtttgaaa aaagatccac tcgatcagtg
    12661 atagctgatt tagcagccaa tacagataca cttgttcctg taactttagg cacagaaata
    12721 tcattaatca aataagagcc aatccaataa atagctaaag tcaatccaga tgaaaccact
    12781 gtcatcacag gattcataag cgacatcaat ctataaacaa aaaggttcaa ttttgttaaa
    12841 tcgtcatttt cacgtttgaa cttatctgtt tgatactttt cagcattgta ggcacgaata
    12901 actctaacac cagagagtgc ctctctagta gttgcattaa gcgcatctgt aaaaccttgg
    12961 atttgacgtt gtctcggaaa agcaagtaag agtagaacgc taagtaatat cataacgata
    13021 ataaccgcta ctccaactgc cccagtccat tgatcgcttt tacccccaat tttagtcaag
    13081 gcccatattg ccataattgg tccacgggta acaacctgca ttcccattac aataaggatc
    13141 tgtagctgag tcaagtcgtt agttgtgcgc gttaatagag aggggataga aaatcgctta
    13201 atttctgcat cagaataatc catcacatgg ttaaaaatat ctgaacgtaa tcgactagta
    13261 aaactagctg ctgtcctagc acctaaaaaa cctaccatta ctgctactaa aaaactacca
    13321 aatgaaaaag caagcatttt tagtccagga tcccaaagag cctctaactt tgtaccttta
    13381 gtttgcaaca tacgtgtgat atcagacata tattctggag tctttaggtc catccaaacc
    13441 gctagacaca ctaatatagt actcgccaca atcatcaacc attctttacc attgagtcgc
    13501 ttgaaaatat taaacatcgc aaactccttt tactttcata tttatcctat tatcctaaaa
    13561 tagttaatca aaatcttcca tcgccatatt tttagctaat gttctaataa cctttttggt
    13621 agtacgtatc tcttctttag taatacctgc taacatggta ctgtgcaatt tttctaagaa
    13681 aatttcaaat tgtgtagcct tttgtttacc tagatgagtc aaataaaggt atttcacacg
    13741 cttatctgtc ttagatggaa caatcgcaat aaatccattt ttttccatcc gttttaccaa
    13801 attagacgct acagacttag aaatgtgtaa aatttcttca gcatctttta tagacatatc
    13861 cttgtcagga tgtttataga gatgcatcac taagtgtcct tgtggacccg ctaaatgttc
    13921 aacatcatac tcttttgcat aacgatctaa atacttttca agttgattaa caagtattcg
    13981 tgctttttga agaggattct ccataataat actcctttaa tagttatcgt gagaactatt
    14041 ttaaaacaac ctttattaat tgtcaattaa aagttaccgt gagaactatt tttacattaa
    14101 tatttctaaa aaacacctat cattcgtcaa ataaaaaatg atttcgctta ctttttaata
    14161 ttaaaaatat atgttcgctt tttatatttt taattataaa tttatattga tttttaatgt
    14221 aaatgatagt aaaatacaaa tgtaatactt atttaattta caggagattt aaatgaaaaa
    14281 gaaaattatt ttgaaaagta gtgtccttgg tttagtcgct gggacttcta ttatgttctc
    14341 aagcgtattc gctgaccaag tcggcgtcca agttataggc gtcaatgact ttcatggtgc
    14401 acttgacaat actggaacag caaatatgcc tgacggaaaa gttactaatg ctggcactgc
    14461 tgctcaatta gatgcttata tggatgatgc tcaaaaagat ttcaaacaaa ctaaccctaa
    14521 tggtgaaagc attagagttc aagctggtga tatggttgga gcaagtccag ctaactcagg
    14581 gcttcttcaa gatgaaccaa ccgttaaaac atttaatgca atgaatgttg agtatggcac
    14641 attaggtaac catgaatttg atgaaggttt ggcagaatac aatcgtatcg ttactggaaa
    14701 ggcccctgct ccagattcta atataaataa tattacgaaa tcatacccac acgaagctgc
    14761 aaaacaagaa attgtagtgg caaacgttat tgataaagtt aacaaacaaa tcccttacaa
    14821 ttggaaacct tacactatta aaaatattcc tgtaaataac aaaagtgtga acgttggctt
    14881 tatcggaatc gttaccaaag acatcccaaa ccttgtctta cgtaaaaatt atgaacaata
    14941 tgaattttta gatgaagctg aaacaatcgt taaatacgcc aaagaattac aagctaaaaa
    15001 tgtcaaggct attgtagtcc ttgctcatgt acctgcaaca agcaaggatg atattgctga
    15061 aggtgaagca gcagaaatga tgaaaaaagt caatcaactc ttccctgaaa atagcgtaga
    15121 tattgtcttt gctggacaca atcatcaata tacaaatggt cttgttggta aaactcgtat
    15181 tgtacaagcg ctctctcaag gaaaagccta tgctgatgta cgtggtgtcc tagatactga
    15241 tacacaagat ttcattgaaa ccccttcagc taaagtaatt gcagttgctc ctggtaaaaa
    15301 aacaggtagt gccgatattc aagccattgt tgaccaagct aatactatcg ttaaacaagt
    15361 aacagaagct aaaattggta ctgccgaggt aagtggcatg attacgcgtt ctgttgatca
    15421 agataatgtt agtccggtag gcagcctcat cacagaggct caactagcaa ttgctcgaaa
    15481 aagctggcca gatatcgatt ttgccatgac aaataatggt ggcattcgtg ctgacttact
    15541 catcaaacca gatggaacaa tcacctgggg agctgcacaa gcagttcaac cttttggtaa
    15601 tatcttacaa gtcgtcgaaa ttactggtag agatctttat aaagcactca acgaacaata
    15661 cgaccaaaaa caaaatttct tccttcaaat agctggtctg cgatacactt acacagataa
    15721 taaagagggc ggggaagaaa caccatttaa agttgtaaaa gcttataaat caaatggtga
    15781 ggaaatcaat cctgatgcaa aatacaaatt agttatcaat gactttttat tcggtggtgg
    15841 tgatggcttt gcaagcttca gaaatgccaa acttctagga gccattaatc ccgatacaga
    15901 ggtatttatg gcctatatca ctgatttaga aaaagctggt aaaaaagtga gcattccaaa
    15961 taataaacct aaaatctatg tcactatgaa gatggttaat gaaactatta cacaaaatga
    16021 tggtacatat agcattatta agaaacttta tttagatcga caaggaaata ttgtagcaca
    16081 agagattgta tcagacactt taaaccaaac aaaatcaaaa tctacaaaaa tcaaccctgt
    16141 aactacaatt cacaaaaaac aattacacca atttacagct attaacccta tgagaaatta
    16201 tggcaaacca tcaaactcca ctactgtaaa atcaaaacaa ttaccaaaaa caaactctga
    16261 atatggacaa tcattcctta tgtctgtctt tggtgttgga cttataggaa ttgctttaaa
    16321 tacaaagaaa aaacatatga aataataaaa ataggtttgg ataaaatatc caaacctatt
    16381 tttatatcaa tatgccttcc aaatgatcta actcgtgttg acaaatttga gctggaaaat
    16441 ctgtcaactt gatttgctgt tctttccaat tgatatcacg ataagaaatc gtaatgtgat
    16501 gatatcgttg tgtacttctg caacctacga gagaaaggca agattcttct gtctcatatg
    16561 gatccgattt tgcaactaat acagggttaa acattactaa atttgtaata cccacattga
    16621 tgataatgac acgcttcaga ctaccaatca tattggctgc catacctaca caataatttt
    16681 gattagcatg caaagtatcc tgtaaatctt ttgctaagct cacatctgct ctactagcca
    16741 tttgtgattt ttgttgtaaa aagaaagtat ctctaacaat tggttttatc atttatttta
    16801 acctaactct attatgataa tactagtata ccataggtta gctaaggact atgataagag
    16861 attaaacaat accttgtgca gacatagctt gagcaacttt taaaaagcct gcaatgttag
    16921 cacctagaac caggttccca ctatcgccaa attcatcagc cgctttggaa gcattcacaa
    16981 aaatagtttt cataatacgt tgcaattctt gatcaacttc ttcaaaagtc catgccctac
    17041 gactagaatt ttgtgccatt tcaagcgctg aaacagctac tcctcctgca ttggcagctt
    17101 tagcaggacc aaaagcaacc cctgcctttt ggaaaacttt aatagcaccc aaggtagatg
    17161 gcatatttgc tccttctgtt actgccaaga caccattttc aactaataac ttagcgtctt
    17221 cttcgtctaa ttcgttttga gtagcacacg ggaaagctaa atctgcttta atagaccaga
    17281 tactaccttg atctgctggt gtgaaattag cttttggatg tttctccgta tatttaacaa
    17341 tacgtgctct ttcaacctct tttatctgtt tcaatgtttc aaggtcaata ccgtctggat
    17401 cataaacata acctgaagaa tctgacacag ctacaacagt tgcacccaac tcttgtaatt
    17461 tttcagtagc ataaatagcc acgttaccag atcccgaaac cagtgcaact ttgccagtta
    17521 aatcttgacc acgcgctgcc agcatttctt tagcaaaata gactgcccca taaccagttg
    17581 cttctgtacg agccaaagag ccaccataag tcagtccttt tccggttaaa accccatttt
    17641 gataaccatt taaacgctta tattgaccat aaagataacc aatttcacgt ccacctactc
    17701 caatatcgcc agctggcaca tctaaatctg gtccaatgta tttttgaagt tcggtcataa
    17761 aactttgtgt gaaacgcatc acttcattat ctgatttccc ttttggatca aagttcgacc
    17821 ctcctttgcc accaccaatt ggtagacctg tcagtgaatt cttaaaaatt tgttcaaatc
    17881 ctaaaaattt gacaatagat tgtgttactg tagggtgaaa acgtaaacca cctttataag
    17941 gaccaatagc tgatgaaaat tgtacacgat atcctcggtt aacttgaact tggcctttat
    18001 catctaccca tggaactcta aaagaaatca cgcgctcagg ctcaaccaaa cgttctaaaa
    18061 gattttcttc aatgtactga gggtatttat caaaaactgg tactagtgac tcaaaaacct
    18121 cttcaaccgc ttgtagaaat tcttcctcat gctcattttg cttcttcact ttctctaaaa
    18181 cacttgctac atatgtcttt ccgcttgtca tttattttcc tccgtattgt tttaatatag
    18241 ttagcattat aaattgtcag aattttcttg tcaactattt gctatggtat cattattagg
    18301 agcaatttta aaggtaatta tttgactttt actgactttc gttctataat gagacaaata
    18361 tttgattgga gtatctatat gaaatggttc ccacttgttg gaggtataat tgccttatta
    18421 gctggcttct atctctttat aaatcctgta acggttgttg caactctggg ttggttcatt
    18481 gctttattta tctttattta tggcatttca aatttagtac gctacttgca agctgattct
    18541 tatcaccgta gcccttggta tctggttcag agtatccttg ctattatttt tggtatcatt
    18601 ttactatcca gctcagcaat gtcactttca aatactgtca tgactatttt agcatactgg
    18661 ctactggtat ctggtatttt acgacttttg ggtggtttcc aaatgcaaaa aattaatttc
    18721 ccaggtgcca aatcctataa acgttcggct tatattgcac tcattttagg tattatttta
    18781 ctggttcaac ctaccttatc agcagccatc attggaagat ttgtagcctt tatattaata
    18841 gcaattggag cttcggctat ttacactttt actcgtatta attattaaaa ggacatgttt
    18901 cgtgtccttt ttttatctat tgatgacgtc atttactatt tgaaaattgg ctattatata
    18961 attcgtaata aaatccttta tctgctagga gtttctggtg attaccttgt tcgatgattt
    19021 gaccatcctt tagaactaaa atgttgtctg cctcttgaat cgttgataga cggtgtgcaa
    19081 tgacaaaact agttcttcct tccatcaatt ttttcatagc tttttgaata agcaattcca
    19141 accttgtatc aacagatgaa gtcgcttcat ctaaaattaa aattttagga tctgctaata
    19201 aagcacgtgc aatagtcaac agttgctttt gtcctaagga gatattgctt gattcttgat
    19261 tcatcaccat attgtagcca ccaggtaacg ttcgaataaa gtggtcaaca ttcgcagctt
    19321 tggcagcagc aactatatcc tcatcactgg cttcaagatt accgaatcta agattttctt
    19381 ttattgtacc ttcatataac caagcatctt gcaacaccat gccaaattgt tgccggaaat
    19441 cttggcgtga taggtgtcta atatcgtgac catctacggt gatagcacct tcactaacat
    19501 cgtagaatct catgagtaag tttataagtg ttgtcttacc tgcacctgtt ggccctacaa
    19561 ttgcaatcat ttgccctggt tctacatcta agttaaagtc gcggataagt ggcttatccg
    19621 gagaatagtg aaaatcgaca ccatgaaagc tgacttgacc ggttaagtca tggctcaatt
    19681 tttcagtgac ttgattggct tcctcttctt catctaagac ttcaaaaata cgttctaagg
    19741 aagattttgc actttgcaaa acacctgcta attgtgtaat ggtttgaacg ggttgactaa
    19801 tttgccaaac atactgcaca aatgcctgca tgttaccaat tgttaaggta ccagcaataa
    19861 cttgtaaacc gccaacaaat gcaatgataa gataaataaa atcagagata ctattcaata
    19921 ctggcatcat aattcctgaa atgaagctag ctttgaaacc tacatgacgt agattatccg
    19981 ttatatcacg aaattcttga ctagacgcct cttctctacc atataatttt atgatattga
    20041 aaccagttaa tttctcttgt acaaaaccat tcagttctcc cagaatcttt gcctgttctt
    20101 taaagtaagg ttgggatctt tttagtatcg cttgtgcact aaaataagtt acaggaatac
    20161 aagcaatgat aatcatggct aatggtacat ttagataaag taccataacg actactagaa
    20221 taatacttaa aaaagcattg ataatctgta aaaagctctg ttgtaaagca tttgagaccg
    20281 tttcaacatc acttgtaaaa cgtcccaaca tatctccaaa ttggtgctta tcaaaatacg
    20341 atacaggaac tttattgatt ttacgattca aatcatgacg gatatcacgg atactttttt
    20401 gaacgacagt agtcataaaa taatttgagc cataagaacc taattcatag aaaacacctc
    20461 tgaaaaaata aataatcaga ataccagcaa tataaggaac gttcaattct gccccagaaa
    20521 ctcccttagc catatcaaca aggttagcag tcaactctgt tatcgctaac cctaaaataa
    20581 aaggctccag aatactcata aaactagata aaactttcaa aaaaatcgct aagaaaagag
    20641 tagctttgta gcgagttagg taagaccata atctcaaaaa aatagatttt tttctcatac
    20701 aatcactctc cttctgataa gttttggttc ttcagttgtg actcagcgat ttcacgataa
    20761 atagcattat tttctattaa ttgagcgtgg gtaccacgac cgacaatttc gccttcatca
    20821 aggacaataa tctgatcagc atccataatc gtacccaccc tttgagcaac tatcaaaacc
    20881 gtagaatcac cggttacttc ttttagacgc gctcttaaag tagcgtctgt cttataatcg
    20941 agagcagaaa atgaatcatc aaaaatatat aaatctggat ctttaacaac agccctagca
    21001 atggataacc gttgtttttg acccccagaa agattgctcc caccttcagc taaatgcgtt
    21061 tcaaaggctt cttggtgact ctcaataaac tctttagctt gagaaatatc aaccgcttgt
    21121 ctaagatcat caatagtagc atcagctttt ccatatttta aattctctcc tattgtccct
    21181 gtaaataaaa gagctttttg ggggataaat ccaatctttt ggcgaagtga tttaaggtta
    21241 taatctctta catcaactcc atctactaag atttttccaa gtgtcacatc ataaaaacgt
    21301 ggaatcaaat taacaagaga agattttcct gaacctgttg aaccaataaa agcaattgtt
    21361 tctccaggct tagctttaaa agaaatatca tgcaaaacgg gactctctgt ttctcctgga
    21421 taagcgaatg ttacattatc aaattctaaa tgccctttaa gtttcgtatc cgtaacacct
    21481 tcggtattag gattaataga aatcggcata tccattacct ctctaatacg atggctagat
    21541 accaccatac gaggatacat agtaaaaaga ttggcaaata gcaaaaatga aaagagagca
    21601 tggaagctat attcgataaa agcaactaaa tcccctattt taatagcacc tctttgtaag
    21661 ggatccaaag caaaccaaac gatagccaca atcattgcaa taataatttg aacgaaaagt
    21721 ggttctgtta gccctgttaa tttaaaaaga ccagttgaag tatctgtgta acgttggtta
    21781 gcgacttgaa atttttgtga ttgaaaattc tctcttgcaa aggccctaac aacgcgtaac
    21841 cctgttaaat tttcacgaac atattgattg attttatcaa gcatagtctg ttgtctttca
    21901 gataaaggtt ttgtttttat agctacatat aaaatgactc ctaccaaaag aggcatcgca
    21961 accgctacaa gccaagccaa agatggactc gtaattagta tcataaccac gctaaaaatc
    22021 attaccatag gagttactag gcctaaacgt aaagacattt cagcaaattg catcaaaaca
    22081 aaagtatcgc ttgtcatacg tgtcactagt gaagatacac ctatctgttc atattcatga
    22141 tgggagtatt cttgaagctt agcatacata tcattacgca tatctctaat cattgttgtc
    22201 gttaagcgac tagatgcgta agtcatcgta atacgcccaa taatacctag tacaacaaat
    22261 ataaacatga tgaacgtcca cagataaact ccagtccgat caccttttgt aacgccatta
    22321 tcaatcatac ccgctaaggc tgtgggcatt cctaggataa ccgtcacaaa aagcatagct
    22381 cctagtaaat caagccataa ccaatttggg taccttttca aataagacca aatatatgcc
    22441 atatatgtct ccttttcttt cctcttatct caaaataatt aatccatgtt cactaatcca
    22501 ctttcaactt ataaatcttt aatcatccaa atatccatag ctgtatgacc ttgatcattt
    22561 gctattggtt gcgataaggc acaatatccc atatgcttat aaaccgcagt tgccctactc
    22621 aaaatactgg ctgtctctaa ataaagttgt ctatacccaa tttttcgagc ttctacttca
    22681 atcattttca ctaaatcagt agcaagcccc ttaccacgga aacgttctgc aatgtacacc
    22741 ttctgcatct ctgcaattag atttttcagc ggaccaaagc cgcttcagcc aataatctca
    22801 tctctctcct caatgacaaa gaatcctgac ttctctattt tttcgtagta tgaggtcaag
    22861 tgatctaagt gagggtctga atatgctgta ccaggtttat ctaaatcata ggcgcgtaaa
    22921 ctctgtcgaa ttaattgtgt aactgcttgg tcatcatttt taataatcgg acggataatc
    22981 atgtgtctaa cccactaagg tactcataac ggtcatactt ttctagaagt gcttcatttt
    23041 ttgcatctaa ttccttttgt aaatcagata accttgtaaa atcactacca catgtttgca
    23101 tatcatcttc tatacgagtg atagtatttt ccaatatcat catatcgtct tcaattgtcg
    23161 cccattcttg tttttcaaag taagacattc ttttacgact ttgcttttca cgacttgtct
    23221 tggtactctc ttttttactg ataacttcat tattttgctc attaaatgct ttttcatcta
    23281 aataatcagt ataattacca aaaaattcac ggatatcgtt atcttcaaat gcaataattt
    23341 tattagccac tttatctaaa aagtaacgat cgtgactaac tgtaatcaca ggaccaccaa
    23401 agccttgtaa aaaattttca agaacagtta atgtagcaat atctaagtca tttgtcggct
    23461 catcaagtag taacacgtta ggcttttcaa tcaggatttt taaaaggtaa agtcgttttt
    23521 tctcaccacc tgataatttt gcaatttgtg ttccatgtgt cgaacgtgga aagagaaatt
    23581 gttccaatag ttctgtcaca cttgttgtac cgacactagt tttaacctca tctgcaacct
    23641 cttgcaaata attaataaca cgttttgagc catccatatt atgaagttgt tgtgaaaagt
    23701 aacctacacg tatcgtttca ccaatagaga cattacccga atccggctgt aaatcttgaa
    23761 caattaaatt aagtaaggtt gactttccaa caccattatc tccaacgatg ccaatacggt
    23821 ctttattttg aattaacaaa ttaaagtctt tcaagataga tttatctggg taagaaaaag
    23881 agacattttc aaaattaata acctttttcc caattcgact tgtttcaaat gtcatttcca
    23941 aatcgcttgt atcgcttgtt tggtgtaaat cgttttttag attttgaaaa cgattaatac
    24001 gagcctgttg tttcgttgca cgagcttgtg gctgagtacg catccaagct agttcctgtt
    24061 tataaagctg tttcttttta tgtaaactag cagcatcacg ctcgtcttgt tctgcacgaa
    24121 gtcggacata atcctgataa ttgccttgat attctgtaat ctgtgcctta tctaattcaa
    24181 aaatacgtgt tgcaacattg tctagaaaat aacgatcatg agttataaaa agcactgtct
    24241 ttttactatt tttcaaaaaa ttcgttaacc atgcaatagt gtcaatatct aagtggttag
    24301 taggttcgtc taagagcaat aaatctgcat catttaataa tacttgcgct aattgaacac
    24361 gtcttcgtaa tcctcctgat aattcaccaa ccgacaactg caaatcagta atacctaatt
    24421 tggataatac tgttttgact tcgctctcaa tagaccaagc atctaaagaa tccatttctg
    24481 ccattacttt ctctagacgt gattgcttac tttcttcgta gtgattaagc aataattcat
    24541 attctttaat taaagccatc tctcttaagt cagaagaaag tacggtgtcc aaaattgtct
    24601 gagaatcatc aaagtctggt tcttgtttta aataagcaat cttataatca ttagctgatg
    24661 aaaaagggga acgatcacca tcaaaaccta attcacccga aataacatct aatagtgttg
    24721 tctttccagt tccattgaca ccaataatcc caatacggtc taaactatgg atgataaatg
    24781 aaacattact aaagaccgtc ttatcaccaa ccgacttagt caatccatct actaaaaaat
    24841 cgctcatata atcctctcta atccatcaag tactgcttaa caaaatgtaa aatagcctct
    24901 tcttcattaa tcagttgtcc taaaacaatg gctaattcaa tttgagagat aatatctccc
    24961 atctgtggac caggttttat tcctaatttc ttgattaagg tgctaccatt gacaactatc
    25021 tcatgcctat catggatagc taactcacta tctagttgtt ctattttttc gaaatcaacc
    25081 atcaagcctc ttgctttgcg aataccttcc gcttgtttta ttatagtttt tccataacga
    25141 taaagatgtt ctttagtcaa tcccatttgt tcacgaatac gataaacatc cactatatgc
    25201 tcaacatctt tttgaaagtg agttgatgtc ttccattttt ttaaaaaagt ctttgtgtgt
    25261 tcaacttcca tactcaagat gagtgatgcc caagcttgtt ctgatgtctc aaataaaaaa
    25321 tctttatcta actgggacaa aaaggcgctt aattgtaatt ccctattttt taagcatggt
    25381 agataattaa aagcatgact atctattagt gccagcattc ctttacgcca ataaggtgct
    25441 aatagaagtt tatcaaattc aataaaagaa cgctcaacag atattttctc caataatggc
    25501 gcgtgctttt tcattgcctc aaaagttgtt gtttcgatat caaaatttag actagcagaa
    25561 aaacgcaaac ctcgcataat tcgaagagca tcttcattaa aacgctcact ggcgagtcct
    25621 actgctctaa gaaggtgatt atctaaatca tccaatccat gaaagagatc aataacttct
    25681 ccatcttcat ttaaagcaaa ggcattaact gtgaaatctc gacgttttag atcttcttct
    25741 agcgacctaa caaaattgac ctgactcgga cgcctataat caacataaac ctcctcagtc
    25801 ctaaaagtgg taatctcata ttcaccacct ttttccaata ctaagactgt tccatgttca
    25861 ataccaacat caactgtcct tttaaaaatc tgctttgttt cttctggata agatgacgtt
    25921 gctatatcaa catcatgaat tggacggtca agtaaaacat cccgaacact accacctaca
    25981 aagtatgctt cataaccggc tttttttatc ttttttaata ttggtaaagc cttctgaaac
    26041 tcagaaggca aataatttaa tctcatcata ctattccaaa tctaaacgaa ctaaagtcgc
    26101 attattattt cttataaatg gattcagaaa caaaaagaac tgcctaaggg acactcccta
    26161 cctactcaaa ttatcaaaaa agaatctcct aacttcttct ttgatatctg caataaggat
    26221 caacctccaa gcaactcttt ttatcacaca tcaatttttt ggggtaatac caaaggcaac
    26281 cgcaccttca ccaagatgag ttgcaataac gccaccaaat gatacaattt ctaaatcgtc
    26341 tttaagacca gctttagcta acaaattata taattgttct gccttatctt gtgctctaga
    26401 gtgaataata gcaatatcat attcaccatc tgctgtcatt tctttgacaa tctcagccaa
    26461 gcgttttaag gcttttttct ctgtacgcac tttctcatag acaacgattt taccttcttc
    26521 attaaaatgt aagaccggct tgatacttaa aaggttacca atgatagctg aaccgtttga
    26581 caatctacca ccttttacta gatgattcaa atcattaaca acaataaatc cttcaatttt
    26641 ttcaatttgt gattgtatct tgttaactat gacatcaaaa tccatccctt cacgtgaaca
    26701 catcaaagca tttctaacta aattgccctg tggtgctgaa gttattttag tatccggaaa
    26761 agcaattgtc aaatttggat gttcttcaat aagaaactga atattctgcc agaaacctga
    26821 tatccccgct gcaatgaata aaccaaggac gtgtgtatac ccttctttct ccaattgaca
    26881 taagagatca tctagttcag ctaagctagg ctgacttgtt ttaggtaatt cttttgatgc
    26941 cgctagctta tcataatatt ggtctaatgt cagattttgt ccctcaatat aagttttacc
    27001 atcaatgata attggaatat ctaaaacata gacatcttca tgattttcga tagcctcagg
    27061 caagtaagct gaggtatccg taataagtgc taatttcatt aactattaaa ctccaatgtt
    27121 actcctggtc tatctaaagc aatttctgta acttcataag tcagacgttt aacgatttct
    27181 aaaagaggag ctgctagatt ctcaacttca tcttgactaa actcactagg ctcattgata
    27241 aggcgccctt ggatatgagc tatttgtgaa atgacaccag aaataacaaa ctcatcacgt
    27301 acaataacaa attgtaacac tgctacaatg ctcgttactt ttgctgcttc atctctatta
    27361 gctaattgga aagtaacttc aaaatttgtt tttggggtac cattttcctc ttcccattct
    27421 aaattgcgcg catcatagtg gtattgattg acaaattctt gttcacgaat gacttccatg
    27481 tattttctcc ttgatagaca atatgtatta ttatagcata aatttgtaga ttcttaaagc
    27541 aggtgggaat ctagaacttc ttcttaggat gtctatcaat cacttcttga ttgttactca
    27601 aggctgctaa acctttttct gtaagtgcca accctctgac aacaaacaac ttccctaatt
    27661 cctcttctgt aaaagcaata gctattgcct gatctaaatc agagcgtttc atctttgaca
    27721 agccttttac gtttttttct tttaagaata gttttaaaag tggagcagat aagtgatcta
    27781 aagaatcaag cggtgactcc attcttacaa atccattttt tattaaaaat tctaggtgtt
    27841 cttttccatt tataccataa atgtattcaa aatattttga aaaacttgtt tcagttgtga
//
LOCUS       DATROD010000016        25842 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.016, whole genome shotgun sequence.
ACCESSION   DATROD010000016 DATROD010000000
VERSION     DATROD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25842
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.016"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(308..694)
                     /gene="rplQ"
                     /locus_tag="VBC74_000677"
     CDS             complement(308..694)
                     /gene="rplQ"
                     /locus_tag="VBC74_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEO6941647.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEASDKYTSTTALQKLFDDIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(709..1647)
                     /locus_tag="VBC74_000678"
     CDS             complement(709..1647)
                     /locus_tag="VBC74_000678"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEO6941648.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELDVEGP
                     AEITAGDILTDSDIEIVNPDHYLFTIAEGHSLKATMTVAKNRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKTEPEMMKVRNLGRKSLEEVKIKLADLGLGLKNDK"
     gene            complement(1697..2080)
                     /gene="rpsK"
                     /locus_tag="VBC74_000679"
     CDS             complement(1697..2080)
                     /gene="rpsK"
                     /locus_tag="VBC74_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEO6941649.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(2098..2463)
                     /gene="rpsM"
                     /locus_tag="VBC74_000680"
     CDS             complement(2098..2463)
                     /gene="rpsM"
                     /locus_tag="VBC74_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEO6941650.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLSTSKKILAAAGISEDIR
                     VKDLTPDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2481..2597)
                     /gene="rpmJ"
                     /locus_tag="VBC74_000681"
     CDS             complement(2481..2597)
                     /gene="rpmJ"
                     /locus_tag="VBC74_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEO6941651.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2623..2841)
                     /gene="infA"
                     /locus_tag="VBC74_000682"
     CDS             complement(2623..2841)
                     /gene="infA"
                     /locus_tag="VBC74_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEO6941652.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(2957..3595)
                     /locus_tag="VBC74_000683"
     CDS             complement(2957..3595)
                     /locus_tag="VBC74_000683"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEO6941653.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTQ
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPTCLIERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPILEHYRKLGLVTDIEGNQEITEVFADVEKALLELK"
     gene            complement(3690..4994)
                     /gene="secY"
                     /locus_tag="VBC74_000684"
     CDS             complement(3690..4994)
                     /gene="secY"
                     /locus_tag="VBC74_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEO6941654.1"
                     /translation="MFLKLLRDALKVKMVRNKILFTIFILLVFRIGTHITVPGINVKS
                     LEQMGELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLFLAFVQSIGITAGFNTLSSVALVKTPNVQTYLLIGAILTTG
                     SMVVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAITTIYEDFFVNVRSSAITNSYIF
                     VGILIVAVLAIVFFTTFIQQAEYKIPIQYTKLVQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFFQNGKEIPWLTKLQELLNYQTPVGMIIYAILIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSIRPGRETEEYMSSLLKKLATIGSVFLAFISLLPIIAQQALHL
                     SSSIALGGTSLLILIATGIEGMKQLEGYLLKRRYVGFMNTTE"
     gene            complement(5015..5455)
                     /gene="rplO"
                     /locus_tag="VBC74_000685"
     CDS             complement(5015..5455)
                     /gene="rplO"
                     /locus_tag="VBC74_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEO6941655.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRMPKRGFSNINAKEYALVNLDQLNVFEDGTEVTPVVLKEAGIV
                     RAEKSGVKILGNGELTKKLSVKAAKFSKSAEAAITAKGGSIEVI"
     gene            complement(5580..5759)
                     /gene="rpmD"
                     /locus_tag="VBC74_000686"
     CDS             complement(5580..5759)
                     /gene="rpmD"
                     /locus_tag="VBC74_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEO6941656.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVNAISHLVTVEEA"
     gene            complement(5774..6268)
                     /gene="rpsE"
                     /locus_tag="VBC74_000687"
     CDS             complement(5774..6268)
                     /gene="rpsE"
                     /locus_tag="VBC74_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEO6941657.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDRNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMVEVPMVGTTIPHEVRSEFGGAKVLLKPAVEGA
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(6287..6643)
                     /gene="rplR"
                     /locus_tag="VBC74_000688"
     CDS             complement(6287..6643)
                     /gene="rplR"
                     /locus_tag="VBC74_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEO6941658.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNIFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSNGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADSARENGLKF"
     gene            complement(6744..7280)
                     /gene="rplF"
                     /locus_tag="VBC74_000689"
     CDS             complement(6744..7280)
                     /gene="rplF"
                     /locus_tag="VBC74_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEO6941659.1"
                     /translation="MSRIGNKVITLPAGVEIINKDNVVTVKGPKGELTREFNKNIGIT
                     VEGTEVTVTRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKALEMRGVGYRAQLQGSK
                     LVLSVGKSHQDEVEAPEGVTFEVPTPTTINVIGINKESVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(7390..7788)
                     /gene="rpsH"
                     /locus_tag="VBC74_000690"
     CDS             complement(7390..7788)
                     /gene="rpsH"
                     /locus_tag="VBC74_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEO6941660.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIADILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGQNGERVITNLKRISKPGLRVYTKHEDMPKVLNGLG
                     IAIVSTSEGLLTDKEARQKNVGGEVLAYIW"
     gene            complement(7943..8128)
                     /locus_tag="VBC74_000691"
     CDS             complement(7943..8128)
                     /locus_tag="VBC74_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEO6941661.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCEKCGRPHSVYRKFQLCRVCF
                     RDLAYKGQVPGVTKASW"
     gene            complement(8146..8688)
                     /gene="rplE"
                     /locus_tag="VBC74_000692"
     CDS             complement(8146..8688)
                     /gene="rplE"
                     /locus_tag="VBC74_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEO6941662.1"
                     /translation="MANRLKEKYTNEVVPALTEKFNYSSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(8712..9017)
                     /gene="rplX"
                     /locus_tag="VBC74_000693"
     CDS             complement(8712..9017)
                     /gene="rplX"
                     /locus_tag="VBC74_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEO6941663.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVVVEGVALIKKH
                     QKPNNENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYNKKSGEVLD"
     gene            complement(9097..9465)
                     /gene="rplN"
                     /locus_tag="VBC74_000694"
     CDS             complement(9097..9465)
                     /gene="rplN"
                     /locus_tag="VBC74_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEO6941664.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9490..9750)
                     /gene="rpsQ"
                     /locus_tag="VBC74_000695"
     CDS             complement(9490..9750)
                     /gene="rpsQ"
                     /locus_tag="VBC74_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEO6941665.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(9776..9982)
                     /locus_tag="VBC74_000696"
     CDS             complement(9776..9982)
                     /locus_tag="VBC74_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEO6941666.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(9992..10405)
                     /gene="rplP"
                     /locus_tag="VBC74_000697"
     CDS             complement(9992..10405)
                     /gene="rplP"
                     /locus_tag="VBC74_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEO6941667.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGTPEGWVAPVKRGKV
                     MFEIAGVSEEVAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10409..11062)
                     /gene="rpsC"
                     /locus_tag="VBC74_000698"
     CDS             complement(10409..11062)
                     /gene="rpsC"
                     /locus_tag="VBC74_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEO6941668.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(11075..11419)
                     /gene="rplV"
                     /locus_tag="VBC74_000699"
     CDS             complement(11075..11419)
                     /gene="rplV"
                     /locus_tag="VBC74_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEO6941669.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKNVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11435..11713)
                     /gene="rpsS"
                     /locus_tag="VBC74_000700"
     CDS             complement(11435..11713)
                     /gene="rpsS"
                     /locus_tag="VBC74_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEO6941670.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(11812..12645)
                     /gene="rplB"
                     /locus_tag="VBC74_000701"
     CDS             complement(11812..12645)
                     /gene="rplB"
                     /locus_tag="VBC74_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEO6941671.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTNTPEKSLLVSLKNKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YILAPKGLEVGQRIISGPEADIKVGNALPLANIPVGTVIHNIELQPGKGAELIRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCSATIGTVGNEQQSLVNIGKAGRNRWKGVRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNQ
                     K"
     gene            complement(12663..12959)
                     /locus_tag="VBC74_000702"
     CDS             complement(12663..12959)
                     /locus_tag="VBC74_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEO6941672.1"
                     /translation="MNLYDVIKKPVITEKSMVALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVTVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(12959..13582)
                     /gene="rplD"
                     /locus_tag="VBC74_000703"
     CDS             complement(12959..13582)
                     /gene="rplD"
                     /locus_tag="VBC74_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEO6941673.1"
                     /translation="MANVKLFDQTGKEVSSVELNEAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVENLSFAAPKTAEFASVLSALSIDSKVLVILEEGN
                     EFAALSARNLPNVTVATATTASVLDIVNADKLLVTKEAISTIEGVLA"
     gene            complement(13606..14232)
                     /gene="rplC"
                     /locus_tag="VBC74_000704"
     CDS             complement(13606..14232)
                     /gene="rplC"
                     /locus_tag="VBC74_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEO6941674.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVVLIKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(14305..14613)
                     /gene="rpsJ"
                     /locus_tag="VBC74_000705"
     CDS             complement(14305..14613)
                     /gene="rpsJ"
                     /locus_tag="VBC74_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEO6941675.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(14820..16100)
                     /locus_tag="VBC74_000706"
     CDS             complement(14820..16100)
                     /locus_tag="VBC74_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO6941676.1"
                     /translation="MRYSKEIIQLAIPAMIENILQMLMGVVDNYLVAQLGVVAVSGVS
                     VANNIITIYQAIFIALGASIASLLAKSLAGSEKDDAISVCSQAIFLTSLIGAVLGIIS
                     IVFGQTFFKLLGTTKSVAQVGGLYLAIVGGGVVTLGILTTLGSFLRVQGQPRLPMYVS
                     IFVNFLNAVLSGFAIFEWRYGLVGVAVSTLIARLIGICILAKYLPIKKIIKRMTWKIS
                     AQIWNLALPSAGERLMMRAGDVVIVAIVVQLGTNVVAGNAIGETLTQFNYMPGLGIAT
                     ATIILTAKYVGQKNRESIEETIQSSYYIGLVLMILISSFMLLAGKPLTQLFTNNPSAI
                     KGSLIVILLSFVGVPATIGTLVYTAAWQGLGNAKLPFYTTTIGMWLIRVVLGYLLGIV
                     FELGLLGVWMATIADNIFRWLFLKVRYHRYIQKM"
     gene            complement(16205..17695)
                     /locus_tag="VBC74_000707"
     CDS             complement(16205..17695)
                     /locus_tag="VBC74_000707"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEO6941677.1"
                     /translation="MAIVYQSTRDASNKVTASQAILKGLADDGGLFTPITFPKVDLDF
                     TKLKDASYQEVAKLVLSAFFDDFTEQELDYCISQAYDTKFDTTEIAPIVKIGDRYHLE
                     LFHGPTIAFKDMALSILPYLLTTAAKKQGVDDKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKHGVSYIQELQMITQAGQNTHVVAIEGNFDDAQTSVKEMFNNSLLRSKLSQ
                     QHMQLSSANSMNIGRLVPQIVYYIYAYAQLVKSKEISIGQLINFSVPTGNFGNILAAY
                     YASQIGLPVTKLICASNDNNVLTDFFKTQTYDKNREFKVTSSPSMDILVSSNLERLIF
                     HLLGDDAETTKKLMEDLVTTGEYALEARQANILESFVAGFATEQFVELDIKRLFDQYQ
                     YIEDPHTAVASAVYQAYQTETKDQTPTVIVSTASPYKFPCVVTKAITNKEEIQDFAAI
                     SILNDLSGVSLPKAVTDLQKAEVIHRMVVPTSNMRETVEECLGLKQ"
     gene            complement(17815..18831)
                     /gene="adhP"
                     /locus_tag="VBC74_000708"
     CDS             complement(17815..18831)
                     /gene="adhP"
                     /locus_tag="VBC74_000708"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEO6941678.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPKVGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLRVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAIVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIAVYGAGGLGNLAVQYAKKVFNAHVVAVDINADKLQLAKEVGADLTVNGKEIK
                     DVAAYIQEKTGGCHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIRVVGSLVGTRKDLEEAFAFGAEGLVVPVVEKVPVDTAPQVFDEMERGLIQGRKVL
                     DFTK"
     gene            complement(19009..21651)
                     /gene="adhE"
                     /locus_tag="VBC74_000709"
                     /gene_synonym="adhC"
     CDS             complement(19009..21651)
                     /gene="adhE"
                     /locus_tag="VBC74_000709"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEO6941679.1"
                     /translation="MTEKTKAVETTDVALAIDTLVQNGLKALDEMRQLNQEQVDYIVA
                     KASVAALDAHGELALHAVEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEEDDV
                     TGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWIEQPSIDATNALMKHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYKEFVEEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKSAVWIAEQAGFTVPEGTN
                     ILAAECTEVSEKEPLTREKLSPVIAVLKAESTEDGVEKARQMVEFNGLGHSAAIHTKD
                     ADLAREFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTDLMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVMTVPGFIAADTGMDVLTHATEAYVSQMANDY
                     TDGLALQAIKIVFDYLERSVKDADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPAATP
                     EEAVESYAKAVYDLGTRLGIKMNFRDQGIDEKEWKEKSRELAFLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYEERPGRRK"
     gene            complement(21920..23698)
                     /locus_tag="VBC74_000710"
     CDS             complement(21920..23698)
                     /locus_tag="VBC74_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEO6941680.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFKNSFPGGFVGVDIFFTFSGFL
                     ITALLIDEFSKTKKIDFVSFCRRRFYRIFPPLVLMVLVTIPFVFLVKSDFRASIGSQI
                     MTALGFTSNFYEILTGGNYESQFIPHLFVHTWSLSIEVHFYVLWGLTVWLLSKRSKDQ
                     KQLRGTLFLVSMGVFGVSFLTMFVRAFFVDNFSTIYFSTLSHIFPFFLGAMVATISGI
                     REITGRFKKNIKNLTLKHNLIMMGSAFAGLMILTFALDFDNRLTYLFGFVLSSIFASV
                     MIYNARILHEHTPDISEPFVITYLADISYGMYLFHWPFYIIFSRLSPNWIAVILTVVL
                     SAVFSTLSFYIIEPFILGRKPKFLDYEFDLLPYKKWLFSIGGVLTLITVVTMLTAPSI
                     GSFETELLQNSLQQARTNTNRTHTLAAGDAGALSDVTVIGDSVALRSSAAFNKLLPEV
                     QLDAAVSRNFSKSFDIFENRIQNKALSKIVVLAVGVNSLDNYKTDLSQFIKSLPKGHR
                     LIIVTPYNAKNMSQVTTVRDYELSLMKKYNYITVADWYKVATEHPEIWGNTDGVHYSD
                     SDTTGADLYVSNVKKAIQKSAQRAAK"
     gene            complement(23695..24075)
                     /locus_tag="VBC74_000711"
     CDS             complement(23695..24075)
                     /locus_tag="VBC74_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941681.1"
                     /translation="MKNFKITRTHLEILSLIIIVVFGLSVFTLTTSSQGVLSYDGGKI
                     KYVGSIVNHHMTGKGKLTYENGDYYKGDFVNGVFEGKGTFVSVHGWSYTGDFKKGQPD
                     GQGRLNAKNKKVYKGTFKQGIYQK"
     gene            complement(24082..24519)
                     /locus_tag="VBC74_000712"
     CDS             complement(24082..24519)
                     /locus_tag="VBC74_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEO6941682.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMANLDKEDSLSVESRATSTWEHG
                     NPIHRGTQAILQRYQIPYTNDKGSQPISKDDLAYFDYIIGMDEQNVKDLKALSQGTYD
                     DKISLFCDGGVPDPWYTGDFEETYRLVSDGCKQWLTLINNSRD"
     gene            complement(24671..25669)
                     /gene="ruvB"
                     /locus_tag="VBC74_000713"
     CDS             complement(24671..25669)
                     /gene="ruvB"
                     /locus_tag="VBC74_000713"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001902090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEO6941683.1"
                     /translation="MTRFLDSDAMGDEELVERTLRPQYLREYIGQDKVKDQLKIFIEA
                     AKLRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAIEKSGDLVAILNDLE
                     PGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGAGETSRSVHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYEENDLTEIIERTADIFEMKITYEAASELARRSRGTPR
                     IANRLLKRVRDYAQIMGDGLIDDNITDKALTMLDVDHEGLDYVDQKILRTMIEMYNGG
                     PVGLGTLSVNITEERDTVEDMYEPYLIQKGFIMRTRTGRVATDKAYEHLGYQRFDK"
BASE COUNT         7782 a         5361 c         4419 g         8280 t
ORIGIN      
        1 attttataaa tttagtatag acataactat tcctctatat tcaattaaga gaaattatat
       61 aaccactatt gagaaatgta gtctagctat taaactcttt gctcatcgaa aatatccaat
      121 aaatataata cataaaacgc ctgcttacga aatataaaca aaattgtttg catttcgtaa
      181 acaagcgtta cctatttaac aatatatgat gagtgttccc gctgagaata attctcagcg
      241 gtagaccaga gctagactaa gaatcgattg attccatcat cataacactc aacaaaattg
      301 ataaaaatta tactaattca ataattgcca ttggggcagc atcgccacgg cgtggttctg
      361 ttttaagaat acgagtgtat cctccgttac gttcagcata acgaggtgcg atgtcgtcaa
      421 aaagtttttg aagagctgtt gttgatgtat atttatcaga agcttcatca tagttttctg
      481 atgcaatttc attacgtaca taagcagcag cttgacgacg agcatgtaaa tcaccacgtt
      541 tacctagagt aatcattttt tcaactgttt tacggatttc tttagcacgt gcttcagtag
      601 ttacaattga ttcgttgata agaagatcgg ttgtcaaatc acgaagcatt gccttacgtt
      661 gtgagctagt gcgtcctagt ttacggtaag ccattatgtc ctcctatttt atttatcgtt
      721 ttttaatcca agacctagat cggcaagttt gattttaact tcttcaagac tcttacgtcc
      781 taagtttcgg actttcatca tttcaggctc agttttttct gttaaatcaa atactgtatt
      841 aattccagca cgttttaaac agttatatga gcgcactgac aaatcaagtt cctcaattgt
      901 gcggtcaagc actttctcat cgttcacttt ctctgtttcc ttcattactt cagttgcttt
      961 agcaacctct gttaaatcag taaacaagtt taagtgttca attaagacgc gagctgaaag
     1021 accaagagca tcctcaggaa tgattgtgcc atttgtcatg atttcaatag ttaatttatc
     1081 aaagccatca ttactaccta cacgagcagg ttcaacttga taattaactt ttttcactgg
     1141 tgtatagatt gaatctacag ccaatgttcc tactggtgca tcatcttttt tatttccttc
     1201 tgctggaaca taacctcggt ttttagcaac ggtcattgtt gctttcaaac tgtgaccttc
     1261 agcaattgtg aaaagataat ggtcagggtt tacaatttcg atatcactat ctgttaaaat
     1321 atctccggca gtaatttctg caggaccttc aacgtctagt tcgataatct tttcgtcttc
     1381 gacgtaagat ttcacagcaa gtccttttac attaaggatg atttgcatca catcttcacg
     1441 tacacctggg atagtatcaa attcgtgtaa tactccatca attttgattg atgtgactgc
     1501 tgcacctgga agtgaagaca gaagcacacg acgaagagag tttcctagag ttgtaccgta
     1561 gccacgttct agtggttcga tgacaaatct gccgtaatct ttattttcat caatttttgt
     1621 tattattggt ttttcaaact caatcattta gttactccct cttaaacgaa aagttgtgta
     1681 ctataaaatg ctatgattat acacgacgac gttttggagg acgagcacca ttatgtggta
     1741 caggagtcac atcacgaatt gcagttactt caagaccagc tgcagcaagg gcacggatag
     1801 cagattcacg acctgaacca ggacctttta cagtaacttc aacagtttta agaccgtgtt
     1861 cttgtgcaga tttagcagca gcttcagcag ccatttgagc agcaaatgga gtagatttac
     1921 gagaaccttt aaatccaaga gcaccagctg atgaccatgc aagagcgtta ccatgcacat
     1981 ctgtaatcat aacaatagtg ttattgaatg tagcgtgaat atgagcaaca ccagattcga
     2041 tgttcttttt cacacgacgt ttgcgtgttg gtttagccaa ttgttttacc tcctatttta
     2101 ttttttctta cctgcaatcg caacagcttt acctttacga gtgcgagcgt tatttttagt
     2161 attttgtcca cggacaggaa gtccacgacg gtgacggatt ccacggtatg aaccgatttc
     2221 catcaaacgt ttaatattca agtttacctc acgacgtagg tcaccttcaa ctttgattgc
     2281 atcaacttca cgacggatag cgtcctcttg atctggtgtc aaatctttaa cgcggatatc
     2341 ttcagaaata cctgcagctg ctaagatttt tttagatgta gaaagaccaa ttccgtatac
     2401 ataagtaagt gaaattacta cacgtttgtc atttggaata tcaactccag caatacgagc
     2461 cattttatct cctttctata ttatccttga cgttgtttgt gttttggatt tgtcggacaa
     2521 attaccataa cacgaccgtt acgacgaata actttacagt attcgcaaat tggtttaacc
     2581 gatggtctta ccttcatgtt ttaatccctc caattatttc gactatttaa agcggtatgt
     2641 gatgcgtcca cgtgttaaat cgtatgggct catttcaaca gtaacacgat cacctaccaa
     2701 aatacggatg tagtttttac ggatttttcc tgatacagtt gccaaaattt ggtgtccatt
     2761 ttccaactca acagtaaaca ttgcgttagg catagtttca acaactttac cttcaatttc
     2821 aatcacatct tcttttgcca cgcaaaagta ccccctaaat ttcgatttga tgtcctctat
     2881 aaagaggtaa caattataag tcagactaac ttagtatatc attactaatc tacttatgca
     2941 agtagattag cataatttat ttgagttcta gcaacgcttt ttcaacatct gcaaaaactt
     3001 ctgttatttc ttgattacct tcaatatctg taacaagacc aagcttacga tagtgttcaa
     3061 gaataggttc tccttgagca atattaacgt ccaagcgacg tttgacagtt tcaggcttat
     3121 catcttcacg ttgatagtaa tcttcttctt tataatctac tggtgggttg aacactttgt
     3181 ggaaagtttc accagtttta cgattgataa tacggccact caaacgctct ataaggcatg
     3241 ttggatccac tttaatatta ataacaccat ctaagcgtag tcctagttct tcaagcgtag
     3301 catctaaggc gtgtgcttgc tcaatagtac gtggatatcc atcaagtaaa aaaccttttt
     3361 ctgcgatatc atcctcagct aagcgctctt ttacaatccc gtttgttact tcatcaggaa
     3421 ccaattcacc tttatcaata taacttttag ctaaacgtcc catttgggtt tgattagcca
     3481 ttgcggcgcg gaacatatcc cctgttgaga tgtgagcaac accaaattct tcaacaatct
     3541 tagctgcttg agtaccttta ccagcaccag gcaaacccat aattaaaaga ttcattatag
     3601 tctccttatt ttgtttttaa ataaatccaa cacagcatgt cggacttatt aaaaaacaaa
     3661 atagaaggtt gacagagtca acctatattc tattctgttg tattcatgaa tccaacatag
     3721 cgtctcttca aaagatagcc ctcaagttgt ttcatacctt caatacctgt tgcaatcaaa
     3781 atgagtaagc ttgtaccacc taacgcaata cttgatgaca aatgcaacgc ttgttgagca
     3841 ataattggta acaaggaaat aaatgctaga aatactgaac caattgttgc taattttttc
     3901 aatagcgatg acatatactc ttctgtttct cgtcctggtc gaatacttgg aatgtaggac
     3961 gaattttttt gaagattctc tgctgtcttt tcagggttta cttgtacaaa agtatagaag
     4021 aaagagaaca gaataatcaa gatagcatag ataatcatac cgactggggt ttgataatta
     4081 agtaattctt ggagtttagt aagccaagga atttctttac cattttgaaa gaaaggaata
     4141 atcgtacttg gaattgttgt aatcgagcta gcaaagataa cgggaataac accagctgga
     4201 tttactttta gaggaagata tgaacttgta ggagctcctt gaacaagttt tgtatattgg
     4261 attggaattt tgtattcagc ttgttgaata aatgttgtaa aaaatacaat tgctaatact
     4321 gctacaataa gaatcccgac aaagatgtaa gaatttgtaa ttgctgaaga gcgtacattt
     4381 acaaagaaat cttcgtagat agttgtaatt gcacttggaa tggaagaaat gatccccgca
     4441 aagataatca tagacactcc atttccgaat cccttatcag taatttgttc acctagccaa
     4501 gtaaccacca tactacctgt tgtaaggatt gcaccaataa gcaaatatgt ttgtacattc
     4561 ggagttttaa ctaaagcaac acttgataga gtgttaaaac cagcagtaat accaattgat
     4621 tgtacgaatg caaggaacaa agaaatataa cgtgtagctt ggttaagctt acgacgacct
     4681 acctctcctt gttttcccca ttcaacaaac ttcggaagaa tatccatttg aagtagctga
     4741 acaacaatag acgcagtaat gtatgggcta actcccatag agaataccga aaagtttctc
     4801 attgcattac cactaactaa gttaagcata tttagaaatg gaagttcacc catttgttct
     4861 aaactcttaa cattgatgcc cggtactgtg atatgcgttc cgatacgaaa tacaagaagg
     4921 ataaagatag taaataaaat tttatttctt accatcttta ctttgagtgc atctctaagt
     4981 aattttaaaa acataatgag ttacctctca ttaattagat gacttcgatt gaaccacctt
     5041 tagcagtgat agctgcttca gcagatttag aaaatttagc tgctttaaca gacaatttct
     5101 tggttaattc accattacca agaattttaa cgcctgattt ttcagcacgc acgattccag
     5161 cttctttaag aacaactgga gttacttcag taccatcttc aaagacattt aattggtcaa
     5221 ggttcacaag agcatattct tttgcattga tgtttgagaa cccacgtttt ggcatacgac
     5281 ggaacaatgg agtttgtcca ccttcaaaac caaggcgtac tccaccacca ctacgcgctt
     5341 tttgtccttt ttgaccacgt ccagaagttt taccattacc tgaagatgta ccgcgaccca
     5401 cacgattgcg gactttacgt gatccttctg caggttttaa ttcatgaagt ttcattattt
     5461 tttctcctta tttgtaaaat gctaacgcct ttacgagggg aaaggtactc cccgcaaaga
     5521 ctcgcctata cgatttgttt aaatcgcacc aataacaatc agtgcgattt agcttttaat
     5581 taagcttctt caacagttac taagtgagag atagcgttaa ccataccacg gatggcagca
     5641 ttatcttcct taacaactga agagtttaat ttaccaagtc caagggcaac aactgtttta
     5701 cgttgttctg gtttgcgacc gattggagac ttagtcaaag taattttaat ttgagccatt
     5761 ataatcccct ttcttatgct aagtcagaaa cagaaatgcc acgtaaagca gcaacttctt
     5821 ctgcacgttt taattgtttc aagccctcaa cagttgcgcg aacaatgttg attggagtgt
     5881 ttgaaccaag tgatttagat gtaatatctg caacacctgc taattcgatg acggcacgaa
     5941 cagcaccacc agcggcaacc ccagcaccct caacagctgg tttcaacaat actttcgctc
     6001 caccaaattc tgaacgaact tcgtgaggaa ttgttgtacc gaccatagga acttcaacca
     6061 tgtttttctt agcagcttca acagctttgc gaatagcttc tggtacttct tgagctttac
     6121 ctgtaccaaa accaacacga ccattacggt caccaacaac tacaagagct gcaaaacgta
     6181 gacgacgtcc acctttaaca acttttgtaa cgcggttaat cgcaacaacg cgctcttcaa
     6241 gttctactgc attatcttta aatgccatta ttaagtgtcc tccctattag aatttcaatc
     6301 cgttttcacg agctgagtca gccaaagctt ttacacgtcc gtgatagaga tatccaccgc
     6361 ggtcaaacac cacttcagaa atacctttag ctactgcgcg ttcagcaaca agtttaccga
     6421 caacaacggc ttgttctgtt tttgttccgt tagaaacttc tttatcaaga gttgatgcac
     6481 ttgcgagcgt tacacccgct acgtcatcaa ttacttgagc gtagatgcct gtattagaac
     6541 ggaaaatgtt caaacgtggg cgatcagcag ttccagagag tttaccgcga acgcgacggt
     6601 ggcgtttttg gcggatttta tttttatctg gtttcgaaat cacaattttc acctcttaaa
     6661 tatattgtta tgtcaaaatg acaatattaa aatgaaaaga tagagaattt gcagaaaaga
     6721 ttctacaaaa tctatcaatc ctattattta cctgttttac cttctttacg gcgtacgaat
     6781 tcaccaacgt aacgaatacc tttaccttta tatggttctg gtgaacgtaa gctacgaacg
     6841 taagcagcag tttgtccaac gctttctttg ttgataccga ttacgttgat tgttgttgga
     6901 gttggcactt caaaagtaac accttctgga gcttcaactt cgtcttggtg agacttacca
     6961 acagaaagaa caagttttga tccttgaagt tgtgcacggt aaccaacacc gcgcatttca
     7021 agagcttttt tgaaaccttc agaaacacca acgaccatgt tgttcaagtt cgcacgagtt
     7081 gtaccatgaa ttgttttcat ttcttttgag tcgttaggac gagtaactgt tacttcagtc
     7141 ccttcaacag tgattccaat atttttatta aactcacgag tgagttcgcc tttaggtcct
     7201 tttacagtaa caacattatc cttgttaatg atttcaacac ctgcaggcaa tgtaattact
     7261 ttattaccaa tacgtgacat gtttattttc tcctgttaaa ttgtcaagct gcttgagcaa
     7321 ctagttttca cggggactca tgagaccaca ggattatact gtgactcaat gagcatttta
     7381 ggtaaaattt taccaaatat atgcaagtac ttctccacca acattttttt ggcgagcttc
     7441 tttgtcagtc aaaagacctt cagaagttga tacaattgca ataccaagtc cgttaagaac
     7501 tttaggcata tcttcatgtt tagtataaac acgaagacct ggttttgaaa tacgtttcaa
     7561 gttagtgata acacgttcac cattttgtcc atatttaagg aacacacgga tgatgccttg
     7621 tttgtcatct tcaataactt caacgttttt tacaaaacct tcacgtttaa ggatatcagc
     7681 aatccctttt ttaatgtttg atgcaggtac ttctaacact tcatgttttg cttggttagc
     7741 attacgaata cgagttaaaa agtctgcaat tgggtcagtc ataaccattt tttaattctc
     7801 ctcttattag cagtttgaat ttaatcactt gctaattaat gagtccttac ggacgttgta
     7861 agactaggtc ttagtatatt tgcgtttaaa aatcagtgcc attatcaatg acacctttta
     7921 aacacaaagc atcattaatt tattaccaag atgctttagt tacgcctggt acttgacctt
     7981 tataagccaa gtcacggaag caaacacggc aaagttggaa tttgcggtaa actgagtgtg
     8041 gacgtccgca tttttcacaa cgagtgtaag cttgcgtaga gaacttcgct gggcgtttgt
     8101 tcttagcaat catagatttt ttagccaatt tatttacctc ctagattact ttgcaaaagg
     8161 cattccaagg ccttttagca attcacgtga ctcttcatca gtgttagcag tagtaacgat
     8221 aacgatatca agaccacgta ctttatcaac gtcatcaaag ttgatttctg ggaagatcaa
     8281 ttgttcttta actccaagag tgtagtttcc acgtccatca aatgatttcg ttggaacacc
     8341 atggaagtca cgtacacgtg gaagtgaaac agaaactaat ttatccaaga attcgtacat
     8401 acgttcacca cggagggtaa cttttgcacc gatcgctaca ccttcacgaa gacggaagcc
     8461 agcgattgat ttcttagctt tagtaataag tggtttttga cctgaaataa gtgctaactc
     8521 agcagcagct ttctcaagat ttttagcgtt tgatacagca tcaccaacac ccatgttaag
     8581 aacgattttc tcaacttttg gcacagccat aacagatgaa tagttaaatt tttctgtcaa
     8641 cgcaggaaca acttcattag tatatttttc ttttaagcga tttgccatta tgcttctcct
     8701 ttccttcgtg attaatcaag cacttcgcct gattttttgt tgtaacgaac ttttttgccg
     8761 tcaacaactt tgtaaccaac acgtcctgct acaccatttt tatcaagaac ttgaacgttt
     8821 gacacatgga ttggagcttc tttttcaacg atagcgcctt gagggttttc gttatttggt
     8881 ttttgatgtt tcttgatgag ggctacgcct tcaacaacaa ctttgtttac ttttggaaga
     8941 gctttaagaa ctacagcttc agtgcctttg tccttaccag caataacgcg aactttgtcg
     9001 ccttttttta caaacatttg agtttttctc ctatttttct tacgcctcga aaggcaccct
     9061 aggaaatcct aggggactac gtttgttaat ctgagattaa agtacttctg gtgccaatga
     9121 aacgatcttc atgtagccac cttcacgcaa ttcacgtgca actgggccaa agatacgagt
     9181 tccgcgagga gttttatcgt cacggatgat tacagcagca ttgtcgtcaa atttgatgta
     9241 tgaaccgtct gggcggcgtg caccagtttt tgtacgaacg ataacagctt taaccacatc
     9301 acctttttta actgctccac caggagtagc ttgttttaca gaagcaacga ttacgtcacc
     9361 gatgttagcg aatttacgtc ctgaaccacc aagtactttg atagtcaaga tctcacgagc
     9421 accgctatta tcagcaactt tcaagcgagt ttcttgttga atcatttcaa ttttctcctt
     9481 ttagtttgat tagataataa cagctttttc cactacttcc acaagacgga aacgttttgt
     9541 agctgaaagt gggcgagttt ccatgatacg aacgatatcg ccttctttag caacgttgtt
     9601 ttcatcatgt gctttatatt ttttagaata gttgatacgt ttaccataga ctgggtggtt
     9661 acgtttagtt tcaactacaa ctgtgattgt tttgtccatc ttgtcagaca ctacgcgtcc
     9721 gtaaagagtt ttacgttgat tacgttccat ttagaatttc tcctttccca atctattatt
     9781 tcatttctga ttgcacagtc ttaacacgtg caatttgttt cttaacttcg tcaaggcgag
     9841 cagtcttttc aagttgacct gctgcagctt ggaaacgaag atcaaacaat tcttttttga
     9901 gttcgttttc tttcttagca agttcttctt gagacaatcc acgaagctct ttaacaaaat
     9961 ctttaatttc ttgaagtttc atgtcttctc cttattctgc ttcacgtttt acgaatttgc
    10021 atttaactgg taatttgtgg ctagcaagac gtaatgcttc acgtgcaact tcttcagaaa
    10081 caccagcaat ttcaaacatc actttaccac gtttaactgg tgctacccaa ccttcaggtg
    10141 tccctttacc agaacccata cgtacaccga tagcttttgc agtgtatgat ttgtgtggga
    10201 agattttaat ccaaacttta ccaccacgtt tcatgtaacg agtcatcgca atacgggcag
    10261 cttcgatttg acggtttgta atccagtggc tagttgtagc ttgaagtccg tattcaccga
    10321 atgaaacttc tttaccacct ttagcctcac cgcgcatttt tccacggaat tcgcgacggt
    10381 gtttaacacg tttaggtact aacatttgtt atttgcctcc tttagtgttt ttacgagctg
    10441 gaagaacttc tccacggtaa atccaaactt taacgccaag tttaccataa gttgtgtcag
    10501 cttcttccca agcatagtcg atatcagcac gaagcgtgtg aagtggaaca gttccttctg
    10561 aataaccttc agcacgagcg atatcagcac cgttcaaacg accagaaact tgagttttaa
    10621 tacctttagc tcctgcacgc attgtacgtt ggattgcttg tttttgagca cgacggaaag
    10681 caacacgttg ctctaattga cgagcaatat tttcaccaac aaggtgagca tctaaatcag
    10741 gttgtttgat ttcaatgatg ttgatgtgta cttgttttcc agttaatttg ttaagttgac
    10801 cacgaagagc gtcaacgttt gctccacctt taccgataac catacctggt ttagcagtgt
    10861 gaagtgaaac aataacttta tttactgcac gttcaatttc aatagttgaa actgatgcgt
    10921 cagctaattc tttattaatg aatttacgga ttgcaagatc ttcatgaagg taatccgcgt
    10981 attctttttc agcataccat ttcgcatccc agtcacggat gattccgaca cgcataccaa
    11041 ttggatgtac tttttgaccc acgattttac ctccttattt ttctgataca actacagtta
    11101 cgtgagttgt acgtttgttg attggtgaag ctgaaccttt cgcacgtgga cggaaacgtt
    11161 tcattgttgg tccttcgtta gcgaaagttt cagatactac caagtttgct ttttccaaac
    11221 caaagttgtt ttctgcattt gcaattgctg agttaagagt tttctcaata acacgagctg
    11281 ctttgtttgg agtgaatttt aagattgcga ttgcgtcagc aacattctta ccacggataa
    11341 gatcaagtac taaacgtgtt ttacgaggtg aaacacgcac tgtacgagcc attgctttag
    11401 ctgaagtaat ttctgccatt gtgtcctcct cctattaacg acgtgttttc ttgtcgtcag
    11461 ctgcgtgacc tttgtaagta cgtgttggtg caaattcacc aagtttgtga cctaccatgt
    11521 cctcttggat ataaacagga acatgtttac gtccatcata aactgcgatt gtgtatccga
    11581 tgaaacttgg gaaaatcgtt gaacgacgtg accaagtttt gattactttt ttcttttcgt
    11641 catttgcttg agcttcaact tttttcatta aatgctcgtc gacgaaaggt ccttttttaa
    11701 gactacgtcc cattttgtag ttttctcctt taaataatgt accacaacgg cctgtacact
    11761 atagtgtact accgagctgg cggatttgta taagattatg ctattaacct attatttttg
    11821 gttacgacga cgaacaataa gtttgtctga tttagctttc ttattacgag ttttaagtcc
    11881 aagcgcaggt ttaccccatg gagtagatgg tgctttacga ccaactggtg ctttaccttc
    11941 accaccaccg tgtgggtgat cgttagggtt cattacagaa ccacgaactg tagggcgaac
    12001 acctttccaa cgattacgtc ctgctttacc aatgttaaca agtgattgct gttcattacc
    12061 aacagtacca attgttgcac tgcaagtacc aaggatcata cgtacttcac ctgattgaag
    12121 gcgaacaaga acatatttac cttcttgacc aagtacttga gcagaagcac cagcggcacg
    12181 aatcaattca gcacctttac ctggttgcaa ttcaatatta tggataactg taccgactgg
    12241 aatgttagca agcggaagtg cgttaccaac tttgatgtct gcttctggac cagaaataat
    12301 gcgttgacct acttcaaggc ctttaggcgc aaggatgtaa gctttaacac catcagtata
    12361 gtgtacaaga gcaatatttg cagtacgatt tggatcgtat tcaattgttt taacaactgc
    12421 ttcaacacca tctttattac gtttgaagtc aatcaaacgg taatgacgtt tgtgtccacc
    12481 accttggtga cgaacagtaa tacgtccgtt gttgttacgt cctgctttat tctttagtga
    12541 aacaagcaat gatttctcag gagtgtttgt agtgatttct gcaaaatcca aagaagtcat
    12601 attacgacgg ccatttgtcg ttggtttata aactttaata cccacgttat ttcctcctta
    12661 gattattcag cttcagcagc aaacaactcg attgcttttg aatcagctgt aagagtgata
    12721 atagcttttt tagtttttga agtaaaacct gtgtaacgac caacacgttt tgctttgggt
    12781 ttaactgtca cagtgtttac acttgcaact tttactccgt caaatgcagc ttcaacagct
    12841 tgtttgatca aaagtttgtg tgcacgtgtg tcaacttcaa atgtatattt ccctgcttca
    12901 agagcaacca ttgatttctc agtgataaca ggttttttga ttacgtcata caaattcatt
    12961 atgcaagaac cccctcgatt gtagagattg cttctttagt aacaagaagt ttgtctgcgt
    13021 taacgatatc aagaacactt gcagtagttg cagtagcaac tgtaacgttt ggaaggttac
    13081 gcgctgaaag tgcagcaaat tcgttacctt cttcaaggat tacaagtact tttgaatcaa
    13141 tgctaagtgc tgaaagaact gacgcaaatt cagcagtttt tggagctgca aatgaaaggt
    13201 tttctacagc tacaaattta tcttcagcaa cttttgctga ataaactgat ttcaacgcaa
    13261 ggcgacgaac tttttgtgga agtttgtagc cgtatgaacg tggagttggt ccaaagacaa
    13321 caccaccacc acgccattgt ggtgagcgga tagaaccttg acgagcgcgt ccagttcctt
    13381 tttgacgcca tggtttacgt ccaccaccag atactgctga acggttttta accgcgtgag
    13441 taccttggcg aaggctagcg cgttggctga ttacaacatc aaaaacaact gattcgtttg
    13501 gttcgatacc gaagatagct tcgttaagct caactgagct aacttcttta ccagtttggt
    13561 caaatagttt aacgtttgcc attttaactg ttttctcctt tcttattatt tagcagcttt
    13621 aactgctgat ttgatagtga taagagattt cttagcacct ggtacgttac ctttgatgag
    13681 gacaacgttt ttctctggga taacttgtac aatttcaagg ttttgaactg ttacacggtt
    13741 accacccata cgtcctgcca agcgtttgtt tttgaaaacg cggttaggcg caacaggtcc
    13801 catagaacct ggacggcgat ggtaacgaga accgtgagcc ataggaccac gtgattgacc
    13861 atggcgtttg ataacacctt ggaaaccttt accttttgat gtccctgtga cgtcaacaac
    13921 atcaccagct tcaaattgtt ctacagataa ttctgcacca acttctaagc cttcaatgtt
    13981 tttgaattca cgaatgaagc gcttaggagc tgtgtttgct tttgcaacat ggcctttggc
    14041 aggtttgtta ctcaatactt cacgtttgtc atcaaaacca acctgaactg cttcgtaacc
    14101 gtctgtttca acagttttaa cttgaagcac aacgtttgga gttgcttcaa tgacagtaac
    14161 agggatgaat tcgcctgatt cagtgaagat ttgagtcatt cccacttttt tccctaagat
    14221 tccttttgtc atgagaaaat atttcctttt cttattttat ttcaaaaagt tttttacgag
    14281 cgttttttgt gctcagaaat caaattaaag tttgatttct acgttgacac cacttggaag
    14341 atcaagtttc atcaaagcat caacagtttt ttgtgttggg ttgatgatgt ctaccaaacg
    14401 tttgtgtgta cgcatttcaa actgttcgcg agaatcttta tatttgtgag tcgcacgaat
    14461 aattgtgtaa agactgcgtt cagttggaag cggaactggt ccagcaactg tagcacctgt
    14521 acgtgttgca gtttcaacga ttttttctgc cgctgtatca agtgtacggt gttcgtacgc
    14581 tttcaaacgg atacggattt ttttgtttgc catcttttct cctctcgtct atttacgata
    14641 ataggctagc tcctcaagaa aaccaacacg agttgcgtgg caatgcaacc tagcgtgtcg
    14701 caacctcttg catcatagct aaagctgttc atacacagca ccagtatatg atatcaaaaa
    14761 aaaacagcac acgcaagcat tattttattt tttttcaaaa aaaatgcaac catttctggt
    14821 tacatttttt gaatatatcg atgatagcga actttcaaaa acagccacct aaagatatta
    14881 tctgctatcg tagccatcca aaccccaagc aaacctaatt caaaaactat tcctaataaa
    14941 taaccaagaa ctactctgat taaccacatg cctatagtcg ttgtgtaaaa tgggagttta
    15001 gcatttccta atccttgcca agctgctgta taaaccaagg tcccaatagt agcaggtact
    15061 cctacgaaag atagcaaaat aacaatcaaa cttcctttaa tggcagaagg attatttgta
    15121 aataactgtg tcaatggttt gccggcaaga agcataaaac ttgatataag gatcattaga
    15181 actaagccaa tataatacga actttgaata gtttcttcta tagattctct atttttttgt
    15241 cctacatatt ttgcagttag gataattgta gcagttgcga tgccaagtcc tggcatataa
    15301 ttaaattgtg tcaaggtttc tccaatagca tttcccgcta caacatttgt acctaactga
    15361 acaacaatag caactataac aacatctcca gctcgcatca tcagacgttc tcctgctgat
    15421 ggtaaggcaa gattccaaat ttgtgcactg attttccaag tcattctttt tattattttt
    15481 ttaataggaa gatacttcgc aaggatacaa ataccaatta atcttgcgat aagagttgat
    15541 actgccactc cgaccaaacc atagcgccac tcaaaaatag caaaacctga taagactgca
    15601 ttgaggaaat tgacaaaaat actgacatac attggtaaac gaggttgccc ttgcacacgt
    15661 aaaaatgagc ctagggtggt taatattcct aaagtaacaa cacctccacc cacaattgct
    15721 aaatataaac caccaacttg tgcgacactt tttgttgtcc ctaaaagttt aaagaaagtt
    15781 tgtccaaaaa caatcgagat aattcctaat actgccccta tcagtgatgt tagaaaaatg
    15841 gcttgagaac atactgaaat tgcatcatcc ttctcactac ctgctaacga cttggccaat
    15901 agacttgcta tactcgcccc taaagctata aaaatagctt gataaatagt aattatatta
    15961 ttagcaactg aaacacctga tactgctaca acacctaact gagccactag ataattatca
    16021 actactccca tgagcatttg taagatattt tcaatcatag ctggtatagc taactgaata
    16081 atctctttac tataacgcac agtatctcct ttctattacc caggatcatt aaaaagagag
    16141 tgatgggaaa atttctaaaa tgtttctttc tcgctctaaa tattttaaaa caatcgtatt
    16201 tttattattg tttcaaacct agacactctt caaccgtttc tcgcatattt gaggtaggta
    16261 ctaccattct atggattact tcggcctttt gtagatctgt aaccgcctta ggtaaagaaa
    16321 cacctgacaa atcgtttaag attgaaatag cagcgaaatc ctgtatctct tctttatttg
    16381 taatagcctt tgtaacaaca cacgggaact tataaggact tgctgttgac acaattactg
    16441 ttggagtttg atctttagtt tctgtctgat aagcttgata aacagctgat gctacagctg
    16501 tatgcggatc ctctatatac tgatactggt caaataaacg tttgatgtct aattcaacaa
    16561 attgttctgt cgcaaatccc gctacaaagc tctctaaaat atttgcttgc ctagcctcta
    16621 aggcatactc cccagtagtc actaaatctt ccattaattt tttagtcgtt tctgcatcat
    16681 cgcctaaaag gtgaaagatt aatcgttcta gattagagga gaccaaaata tccatagagg
    16741 gacttgatgt cactttaaat tctcgatttt tatcgtaggt ttgtgtctta aaaaaatctg
    16801 ttagaacgtt attatcattt gaggcacaaa tgagtttggt aacaggtaat ccaatttgac
    16861 tagcataata tgctgctaaa atattaccaa aatttcctgt aggtacacta aaattaatta
    16921 gttgaccaat tgagatttcc tttgacttaa ctaattgagc atatgcatat atatagtaaa
    16981 caatttgagg aacaaggcga ccgatgttca ttgaattggc tgatgataac tgcatatgct
    17041 gctgtgataa ttttgatcga agaagggaat tattgaacat ttctttaacc gaagtctgtg
    17101 catcatcaaa atttccttca atcgcaacaa catgggtatt ttgcccggct tgtgttatca
    17161 tctgcaattc ttgaatataa ctaacaccat gtttaggata aaatacaata atctcagttc
    17221 ctggaacatc tgcaaaacct gccatcgctg ccttaccagt gtcacctgat gttgctgtta
    17281 aaataacgat tttatcatca accccttgtt ttttggctgc tgttgttagc aaataaggaa
    17341 gaatggataa agccatatct ttaaatgcaa ttgtaggacc gtgaaataat tcaaggtgat
    17401 accggtctcc aatctttact attggggcta tttcagttgt atcaaattta gtatcataag
    17461 cttgactaat acagtaatcc aattcttgtt cagtaaaatc atcaaaaaaa gctgacaaaa
    17521 cgagtttagc cacttcttgg taggaagcat ccttaagttt agtgaaatct aaatcaactt
    17581 ttggaaatgt gatgggggta aagagtcctc catcgtcagc taatcccttt agaatcgctt
    17641 gactagctgt cactttatta ctagcatcac gtgttgattg atatactatt gccataagaa
    17701 tccctcttta tataaatatc ttttagttat tgtaacacaa tttaataaca tgtccattgt
    17761 tcgttatata attaaccccc ctttattaaa ataaaggggg gcgtaatatc attatcactt
    17821 tgtaaagtcg agaactttac gtccttgaat caatcctctt tccatttcat cgaatacttg
    17881 aggagctgta tccacaggca ctttttcaac cactggtact accaaacctt ctgctccaaa
    17941 tgcgaaggct tcttctaaat ctttacgagt tccaactaag gaaccaacca cacgaatccc
    18001 atcaagtact gtttttacaa tactcaattc catatattct gatggaagac caacagctac
    18061 cactgtacct ccagcacgaa cgctgtcaat cgcctgatta aacgcaactt tagaaacagc
    18121 agtcacaacc actccatgac aaccgcctgt cttttcttga atataggccg caacatcttt
    18181 tatttctttg ccattaactg tcaaatctgc tccaacctct ttagctaatt gaagtttatc
    18241 tgcgttaata tcaacagcta caacatgagc attgaatact ttttttgcat attggactgc
    18301 taagtttcca agaccacctg caccatacac tgcaatccac tgaccaggag cagctccagc
    18361 ttctttgata gccttgtatg ttgttactcc agcacaagtg attgatgatg cttgagctgg
    18421 atctaatccc tcaggaacct taaccgcata gtccgcggta acgatagcgt attcactcat
    18481 accaccatca acactgtatc cagcattttt aacactacga caaagcgtct cacgtcctgt
    18541 agtacagtat tcgcaatgac cgcacccttc aaagaaccat gcaatagaga cacgatcacc
    18601 aaccctcaaa gacgttacgc cttctccaat ttcttctact ataccaatgc cttcgtgacc
    18661 caaaatgcgt cctggtacct ggccgaaatc tccatgtgca acgtgtaaat cagtatggca
    18721 cacaccacag tattctactt ttacaagtgc ttcaccatga ccaacttttg gaagatcatg
    18781 ctcaacaact tcaacacctg tactagcttg attaacaaca accgctttca tgataaaacc
    18841 tcctagaaat aaatatatct tatttgtaat cgtttacatt ttatattgta ttattattca
    18901 caaactatgt caagtgattt tcagaaaatt ttttgaaaat attattaact ggttaatctt
    18961 tttgaaacta aaaatcccac tcaatagagt gagattcttc gactgttgtt acttacggcg
    19021 tcctggacgt tcctcataac cataataagc atcttcgatg atttcttgca tgtgatctac
    19081 cattggaagg cgtggatttg ctggtgaaca ttgatcttca tatgctaaga aagctagttc
    19141 acgagatttt tctttccatt ctttttcatc aatgccttgg tcacggaaat tcatcttaat
    19201 acctaaacgt gttccaaggt catacacagc ttttgcatat gattcgacag cttcttctgg
    19261 tgttgctgct ggtaagccga gtaatttagc gatatcttga tacttttcat cagcacgata
    19321 gtaattgtac ttaggccatg ttgctgtttt agctggacga gttccattat agcggataac
    19381 atacggcaaa agaattgcat ttgtacgtcc gtgtacggta tggaattgtg cgccaatttt
    19441 atgcgccatt gagtgagaaa tacctaagaa tgcattggcg aaagccatac ctgccatagt
    19501 tgacgcattg tgcatctttt cacgtgcttc aaaatcagca tcttttactg agcgttctaa
    19561 gtagtcaaat acaattttaa ttgcttgaag agcaagtccg tctgtataat catttgccat
    19621 ctgtgaaaca taagcctctg ttgcatgtgt caatacatcc atacccgtat cagccgcaat
    19681 aaaaccagga acagtcataa caagggccgg atcaacgata gctacagttg gtgttaatga
    19741 ataatcagcg attggatatt tacgattatt tgctttgtca gagataacgg caaatggtgt
    19801 cacttcagaa cctgttccag aagttgttgg aatagcaaca aattttgttt tcttaccaag
    19861 ttctgggaat ttgaacgcac gtttacggat atccatgaat ttttgaacga ggtcatggaa
    19921 atcaacttct ggttgttcat agaagagcca cataactttc gcagcatcca taggagaacc
    19981 accacctaat gcaataatag tgtctggttt gaaggtacgc attaaatcag tccctttcat
    20041 tactgttgta atgtctggat ctggttctac ttcagcaaaa atctgataaa caactttatt
    20101 gcgacgaaga tcaagttgtt caatgatacg atccaagaaa ccaagctcaa ccatggcgtg
    20161 atctgtaaca atcatgacac gttcaacatc gcgacatttt tgcaaatatt gaatagaatc
    20221 acgttcgaag tatgtttttg aagggacttt aaaccattgc atattatttc tacgtcttcc
    20281 tacttttttg atattcaaga gatttatagc actaacgtta tcaccaactg agttacgtcc
    20341 atacgaacca caaccaagag tcaatgatgg caagaaagca ttgtaaacat ccccaatacc
    20401 accaaaagta gaaggagaat tccaaataac acggatagca cgaattctag ttccaaattc
    20461 acgagctaag tctgcatctt ttgtatgaat agcagctgag tgccctagac cattaaactc
    20521 aaccatttga cgagcttttt caacaccatc ttctgtagat tcagctttca atactgcaat
    20581 aacaggtgat agtttttcac gtgtcaaagg ttctttttca ctaacttcag tacattctgc
    20641 ggcaagaata tttgttcctt ctggaacagt aaatccagct tgttcagcaa tccaaactgc
    20701 tgattttcca acaatatttg gatttaattt tgcaccagca cagtttttgc tatttgcttt
    20761 tgcaccaaaa cagaattctt caagaagagc tttttctttc ttattaacga aataggtatg
    20821 ataagattta aattcttcaa caaattcttt ataaatttct ttatcaataa taacagcctg
    20881 ttctgacgca cataccatac cgttatcaaa tgatttagac ataacaatat cgtgagcagc
    20941 ttgacggata ttagctgatt tttcaacata agctggaaca ttcccagcac ctacaccaag
    21001 ggcaggtttc ccacatgagt aggcagcttt aaccatcgca ttaccacctg tcgcaaggat
    21061 agtggcaata ccatcatgtt tcatcaaagc attagttgca tcaatcgatg gttgttcaat
    21121 ccattggaca caattttcag gagctccagc agcgatagca gcatcacgaa caatacgtgc
    21181 tgcatgtgct gatgattctt gtgctgatgg gtggaaggca aaaataattg gattacgtgt
    21241 tttaagagag attaatgact tgaaaatagc agttgaagtc gggttggttg ttggcgtaat
    21301 accacagaca actcccactg gttcagcaat taatgtcaag cctgtgacat cgtcttcttc
    21361 aataactccc actgtctttg tatgacgcat gttgttaacg acgtgttcac atgcaaataa
    21421 attttttgtc gctttatctt caaagacacc acgaccagtt tcttctactg catgaagagc
    21481 aagttctcca tgggcatcca aggctgcaac agaagcttta gctacaatat aatcaacttg
    21541 ttcttgatta agttgacgca tctcatcaag tgcttttaaa ccgttttgaa ctaatgtatc
    21601 aattgcaaga gcaacatctg tagtttctac cgctttagtt ttttcagtca tttgagactc
    21661 cttttgattt gttaaatgtt tcacaaattt gtttacgaga ttattatagc atatttaaaa
    21721 actttgtaaa tagtttcaca acttttttgt gaactttttt tcaatctttc ggaaacattt
    21781 cttgaaagcg ctttaattaa atttgttttt tcattactaa ttttcagatt aaagccaatc
    21841 gatttcattt gtatcaccat aaacggaggt atctattatt cgttttcaca aaaaagccga
    21901 gtacatgact cggacttgtt tattttgcag ctcgttgagc tgatttttga atagcttttt
    21961 tgacgttact aacatataaa tcagcccctg tagtatcgct atcactataa tgcacaccat
    22021 ctgtatttcc ccagatttct ggatgttctg tcgcaacctt ataccagtca gcaaccgtta
    22081 tgtaattata ttttttcatt agcgagagtt cataatcacg cactgtcgta acttgactca
    22141 tatttttagc attataaggc gttactataa tcaaacggtg tcccttaggc aaagatttaa
    22201 taaattggct caagtctgtc ttgtagttat ctaatgaatt cacaccaact gccaaaacaa
    22261 caattttgga caaggcttta ttttgaatac ggttttcaaa aatatcaaat gatttactaa
    22321 aattacgact caccgcagca tctaattgta cttctggtaa caatttatta aaagcagcac
    22381 ttgaccttaa ggcaactgaa tctccgatta ccgtcacatc actaagagca cctgcatccc
    22441 ctgcggctag tgtatgtgtt cggttagtat ttgttctagc ttgttgtaac gaattctgca
    22501 ataattctgt ctcaaaactt cctatacttg gagctgtcaa cattgtcact acagtaataa
    22561 gagttagtac ccctccgata ctaaataacc actttttgta aggcaacaag tcaaattcat
    22621 aatcaaggaa tttaggtttt ctacctaaaa taaaaggttc aatgatataa aatgataatg
    22681 ttgagaagac tgctgataat actactgtca aaataacagc tatccagttt ggggataaac
    22741 gactaaaaat aatataaaat ggccaatgaa aaaggtacat accgtaactg atatcagcaa
    22801 gataagtaat cacgaaaggt tctgaaatat ctggtgtatg ctcgtgtaaa atacgtgcat
    22861 tgtaaatcat tacactagca aaaatgctag acaaaacaaa accaaaaaga tatgttagcc
    22921 gattatcaaa atcaagtgca aaagttaaaa tcattagacc agcaaaagca ctacccatca
    22981 tgattaaatt atgcttcaaa gtcagatttt tgatattttt cttgaatcgt ccggtaatct
    23041 ctcgaatacc agatattgta gccaccatcg ctcctaaaaa gaacggaaat atatgtgata
    23101 atgtagagaa atatatcgtt gaaaaattat caacgaaaaa ggctctaaca aacatggtaa
    23161 ggaagctgac tccaaaaact cccatggaaa caaggaacaa agtccccctc aattgctttt
    23221 gatcttttga tcttttcgat aagagccata ccgttaagcc ccataaaaca taaaaatgga
    23281 cttcaataga aagactccaa gtatgcacaa ataagtgagg gataaattga ctttcgtagt
    23341 taccaccagt caagatttcg taaaagttgc tggtaaaacc taaagcagtc ataatttgag
    23401 agccaatact tgctctaaaa tcgcttttaa ccagaaatac aaaaggtatt gtcaccaaaa
    23461 ccatcaatac taatggtggg aaaatacggt aaaaacgtct acgacaaaaa ctaacaaaat
    23521 caattttttt cgttttagaa aattcatcaa tcaacaaggc cgtaatcaga aaaccagaaa
    23581 atgtaaagaa gatgtcaaca ccaacaaatc ctcctggaaa actattctta aagaaatggt
    23641 aaagtaaaac aagtagtagt cctgttatac gtaccaaaga aaaccatttt attctcattt
    23701 ttgataaatc ccctgtttaa atgtcccctt atagactttc ttatttttag cattgaggcg
    23761 accttgtcca tcaggttgac cttttttaaa gtcacctgta taagaccagc catgaactga
    23821 aacaaatgtt cctttccctt caaagacccc atttacaaaa tcgcccttat agtaatcccc
    23881 attctcatag gttaatttac ctttaccagt catatggtga ttaacaatag agcccacata
    23941 ttttatctta ccgccatcat agctaaggac tccttgacta gaagtcgtta aggtaaatac
    24001 tgataaacca aaaacaacaa taattatcaa agataatatt tctagatgag ttcgtgttat
    24061 tttaaaattc ttcatttcta cttaatctct actattattt attagcgtta accactgttt
    24121 acagccatca ctgactagac gataggtttc ctcaaaatca ccagtatacc atggatcagg
    24181 aacaccacca tcacaaaaaa ggctaatctt atcatcatag gttccttgtg atagagcttt
    24241 taaatccttg acattttgct catccatacc gatgatataa tcaaaatatg ctaaatcgtc
    24301 tttagaaata ggttgtgacc ctttatcgtt agtgtaagga atttgataac gctgcaaaat
    24361 cgcttgcgtt cccctatgta taggatttcc atgctcccat gttgaagtcg cgcgactctc
    24421 aaccgagagg ctatcctcct tatctaaatt cgccataaca aactcagcca tagggctacg
    24481 gcagatattt ccaagacaaa caaaacagac tttcttcata gcaaactcct tttggtctat
    24541 tataacagaa aaaaggtact tataatacgt ttaactgctc aaagaataac tacaacatat
    24601 ctaatactta acattgcatg aaaattaaaa aattaagact agatacccta gccttaaaat
    24661 gacttatgtt ttatttatca aatcgctgat aacctaaatg ttcgtatgcc ttatccgtag
    24721 ctacacgacc ggtacgggta cgcataataa aacctttttg aattaaataa ggttcgtaca
    24781 tgtcttcaac agtatctcgt tcttcagtaa tattaacgga tagagttcct aaaccaacag
    24841 gacctccatt atacatttca atcatggttc ttaagatttt ttgatcgacg taatcaagcc
    24901 cctcgtgatc aacatctaac atcgttaatg ctttatctgt aatattgtca tctatcaaac
    24961 catctcccat gatttgagca taatctcgaa cacgtttcaa taaacggtta gcgatacgtg
    25021 gcgttccacg actgcgacgc gctaattcag aagcagcttc ataagtaatt ttcatttcaa
    25081 aaatatctgc tgtacgctca ataatttctg tcaaatcgtt ttcttcataa tactccatat
    25141 gccctgtaat accaaagcga gcacgtaagg gattagataa catacctgca cgtgtcgttg
    25201 caccaattaa ggtaaagggc ggcaaatcta aatgaacact tctactagtt tctcctgcac
    25261 cgatcataat gtcaatatag aagtcttcca ttgcactata aagtacctct tcaaccgcca
    25321 tcggcatacg atgaatttca tcaataaaaa gaacatcacc tggttctaaa tcatttaaaa
    25381 tggctactaa atcccctgat ttttcaattg cgggacctga tgtttgtttg agattgacac
    25441 ccaactcatt agcaattaca aatgccatgg ttgttttccc taaaccagga gggccaaata
    25501 ataacacatg atccaatgac tcatcacgca atttagcagc ttcaataaat atttttagct
    25561 gatctttaac cttatcttgt ccaatatact ctcttaaata ctgcggacga agtgtacgtt
    25621 ctaccaattc ttcgtcaccc attgcatcac tatctaaaaa tcttgtcatg atattattat
    25681 aacatttgta taggaaaagt taagaactaa gttggtgtat tagtatctcc cttatttaaa
    25741 ggaagaataa actgatctta tagttaatcc aacctcaaga aatcacagaa cagcttactt
    25801 agcccatatt ccaccaaccc attcctaaaa acaaatcaaa tg
//
LOCUS       DATROD010000017        25780 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.017, whole genome shotgun sequence.
ACCESSION   DATROD010000017 DATROD010000000
VERSION     DATROD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25780
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.017"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            135..1427
                     /locus_tag="VBC74_000714"
     CDS             135..1427
                     /locus_tag="VBC74_000714"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEO6941684.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDNKKFKLHLIPSGIFFKEKISVIGNGVVVNPKSLVKELAYLHGEGVTTDNLRISDRA
                     HVILPYHIKLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDREVFAERL
                     KINLAEKNRLFEKMYDSTPLEFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGDRIREIGKEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGKRIDYYPASLEQLKRCKPIYEELPGWSEDITACRSLDDLPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWSNI"
     gene            complement(1450..1743)
                     /locus_tag="VBC74_000715"
     CDS             complement(1450..1743)
                     /locus_tag="VBC74_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941685.1"
                     /translation="MILINLIALPICFLAELSAGLLLKLEMHKQHGYYPLERLLMTGS
                     ILTVVSLVLLHTLIHNINSLLFLSIIVVLLISGKSLSLISIYLLKRKSKHQKV"
     gene            2082..2699
                     /locus_tag="VBC74_000716"
     CDS             2082..2699
                     /locus_tag="VBC74_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941686.1"
                     /translation="MRKFFQDVIVEVAYYLEIALSVILATAIIFFSLALFHDLFSMLS
                     VDQNVDKLFQTFLARAMTIAVGIELIKMFSKPSPDTLIEVLLFALARQLVIEHGSVSD
                     YLMGIASVAILFAVRKYLFTHFDESSNIVLRANNKVKKANILARVQLPCQPNETLGDL
                     LVRHLNDEEKDVAIGSVVYINNVALRVDSMHDGLVTRVEIIRSLH"
     gene            2867..4306
                     /locus_tag="VBC74_000717"
     CDS             2867..4306
                     /locus_tag="VBC74_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6941687.1"
                     /translation="MENFLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPLHDVFAG
                     FIKATVGYLILNVGAGGLVNTFRPILVALAKKFHLEAAVIDPYFGLASANAKLETMGF
                     ISVATTALLIGFGINILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQLRNG
                     FGAWAVGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGGILAVLGPDIMSNVKLIGPGAFVPTKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVTELTNAFQGISNKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVVFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGIIQVALGAVAVGLLGLAGGYHGNIDFEFPWLAFGYIFKYLGIAGYVIV
                     CLFFLAIPQLQFMKSKDKEAYYRGDAPAE"
     gene            4334..4612
                     /locus_tag="VBC74_000718"
     CDS             4334..4612
                     /locus_tag="VBC74_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6941688.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVSNFESASCSVGEA
                     KGLAANYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEIL"
     gene            4679..5164
                     /locus_tag="VBC74_000719"
     CDS             4679..5164
                     /locus_tag="VBC74_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6941689.1"
                     /translation="MNLKQAFIENDSIRLKLSASDWKEAIKLSIDPLIESGAVDAEYY
                     DAIIESTEEFGPYYILMPGMAMPHARPEAGVKRDAFSLITLTEPVVFPDGKEVSVLLA
                     LAATSSAIHTSVAIPQIIALFELENSIQRLTECQEAKEVLAMVEESKNSPYLEGLDLE
                     S"
     gene            5277..5942
                     /locus_tag="VBC74_000720"
     CDS             5277..5942
                     /locus_tag="VBC74_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEO6941690.1"
                     /translation="MTKRLPNLQVALDHSDLQGAIKAAVSVGHEVDVIEAGTVCLLQV
                     GSELVEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKEERGDRGEIQIELYGDWTYEQAQQWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLSTDTLQLFEGVDVFTFIAGRGITEADDPAAAARAFKDEIKR
                     IWG"
     gene            5946..6809
                     /locus_tag="VBC74_000721"
     CDS             5946..6809
                     /locus_tag="VBC74_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEO6941691.1"
                     /translation="MTRPIGIYEKATPKHFNWLERLQFAKELGFDFVELSIDESDERL
                     ARLEWSKEERLELVKAIFETGVRVPTITFSGHRRFPMGSNDPEKEARAMDMMKKCIVF
                     AQDIGIRNIQLAGYDVYYEEKSPETRARFIKNLRQACTWAEEAQVILSIEIMDDPFMN
                     SIEKYLAVEKEIDSPYLFVYPDTGNVSAWHNDLWSEFYNGHRSIAALHIKDTYAVTET
                     SKGQFRDVPFGQGCVDWEEMFAVIKKTNYNGPFLIEMWSENCETVEETRAAIKEAQDF
                     LYPLMEKAGVK"
     gene            6811..7527
                     /locus_tag="VBC74_000722"
     CDS             6811..7527
                     /locus_tag="VBC74_000722"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEO6941692.1"
                     /translation="MAKSLQEMRERVCEANKSLPVHGLVKFTWGNVSEVDREAGLIVI
                     KPSGVDYDQLTPENMVVTDLEGNIVEGDLNPSSDLPTHVQLYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLSEDEVNTAYEKETGSVIIEEFERRNL
                     DPMAVPGIVVRNHGPFTWGKDPAQAVYHSVVLEEVAKMNRFTEQINPRVEPAPKYIMD
                     KHYLRKHGPNAYYGQKGQEH"
     gene            7538..8185
                     /gene="fsa"
                     /locus_tag="VBC74_000723"
     CDS             7538..8185
                     /gene="fsa"
                     /locus_tag="VBC74_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEO6941693.1"
                     /translation="MKYFLDTADVSEIRRLNRLGIVDGVTTNPTIISREGRDFKEVIN
                     EICQIVDGPVSAEVTGLTCDEMVTEAREIAKWSPNVVVKIPMTEEGLAAVSQLSKEGI
                     KTNVTLIFTVAQGLSAMKAGATFISPFVGRLEDIGTDAYALIRDLRHIIDFYGFQSEI
                     IAASIRGLAHVEGVAKCGAHIATIPDKTFASLFTHPLTDKGIETFLKDWDSFKKK"
     gene            8348..9331
                     /locus_tag="VBC74_000724"
     CDS             8348..9331
                     /locus_tag="VBC74_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6941694.1"
                     /translation="MFEKVTISDIANLVGVSKATVSYYLNGNYKKMSLQTKEKIRLAI
                     KETGYQPSKIAQSLVTKNTRTIGVVIADITNPFISSVMKGIHDTCQQFGYSVNFTNSD
                     NDIDIELENLNRLNQQNVSGIILDSVDPNHSFIETLSNDRLVMVDRQAKDIKVDTVVS
                     DNKESTQIFLEKMQETGYHDIYFVTYPIEGISTRELRYEGFKEVVSSNPDKLIIITED
                     GSTQRILDIIEHSEQKPGFLMMNGPTLLNFMKKLNQSTVSYPEDYGLGSYEDLEWMQV
                     LTPNVSCIKQDSYGIGCLAAQCLIEKISQGNEPTTARLLEVKNQIVIRQSF"
     gene            9566..10585
                     /locus_tag="VBC74_000725"
     CDS             9566..10585
                     /locus_tag="VBC74_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941695.1"
                     /translation="MKINKLLSDRLEACYSVCRLQNQDYDFLLVASEVDQACFAYDLN
                     SDLAKIVVWEDVGGTMSIIPIPGTMDFLATQKFYPGFKAKDCQVVYASFQGDHWDICN
                     AFDFPYLHRFDLIKGKENQLYFIGCTIANSKAYTEDWSDKGRIFVARFNTQNHTLEGL
                     QQLPQTLLKNHGYYAIQDEGYSLITSVEGVLKLTYPEFSTTGDWQLERLFDEETSDVV
                     KVDINRDGKDEYVIIQGFHGDRLRIFTEDFGRELFHYPEKTPFGHAIWSGRLLNQTCF
                     VFGWRSEKAELRLFHFVDGHLVSELVDAKAASSNVLAFEKDGKAYLFSANNGRGEVAL
                     YQLVK"
     gene            10604..11560
                     /locus_tag="VBC74_000726"
     CDS             10604..11560
                     /locus_tag="VBC74_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO6941696.1"
                     /translation="MDKKKILVTGIVPKEGLRKLMDRFDVTYSEDRPFSRDYVLEHLS
                     EYDGWLLMGQKGDKEMIDAGENLQIISLNAVGFDHVDTAYAKEKGIVVSNSPQAVRVP
                     TAEMTFALILAASKRLAFYDSIVRSGEWIDPSEQRYQGLTLQGSTLGIYGMGRIGLTV
                     ANFAKAFGMTVVYNDVYRLPEDKEKELGVTYLEFDQLIETADVITIHAPALPSTIHKF
                     NKDVFAKMKNRSYLINAARGPIVSEEALIEALKEGEIAGAGLDVFENEPQVSEGLRSL
                     DNVIMSPHAGTGTIEGRRTLAEEAADNIIAFFDGKPQNIVNK"
     gene            11658..13109
                     /locus_tag="VBC74_000727"
     CDS             11658..13109
                     /locus_tag="VBC74_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEO6941697.1"
                     /translation="MLEALHKFFEVFGAATVVPAMIFIVSLFLKVNPRRAFFSALRAG
                     VGLTGFGWIISAFAPMVTKYIQQMVKTTGLHLPIVDIGWQAGSLTAFSSEIGLSFFVF
                     GLLIELGLFLLGITRVFVPSNLWNNFGYMIWGTMAYAATGNFILSFAFMVFVLLYSLV
                     MSEVLADRWSEYYGVKNATINSIHNIETLIPALILDPLWNLLGVNKVKLNPESLKTKL
                     GIFGEPMTLGFILGVIIGVLGSLRNLASIDTWGGILGFAVALAAVMTIFPLITGVFAS
                     AFAPLAEAVERNKKKESQAEQGALDKKRWFIAVDDGVGFGEPATIIAGLILVPIMVVI
                     SLILPGNEALPVVDLIAIPFMIEAMIAVSKGNILKAILNGIIWFSLGLYAASALGPIY
                     TEAVKHFGTALPAGVTLIMSFNLLAHPLTALVFFAWISGNPLYIGVTVIIYLAFLFLL
                     RTRRESIYAYLKTMADKNSGYEGSCEKIFIAED"
     gene            13131..14120
                     /locus_tag="VBC74_000728"
     CDS             13131..14120
                     /locus_tag="VBC74_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO6941698.1"
                     /translation="MKKIDGHAHIVDTIAGFNGKGRLNALGNGYAIWDDGDLIKLIPD
                     EYGDKAFTAESFLKYMDANGVDKAVILQGHLNGYQNYYTHLAIKRYPERFTGAFSVDP
                     FADNAMQIVKRHVEVLGFRAIKFEISQGGGIHGYRGQKTPFRLDTDPHVSRILTYLLD
                     YPGFVVTVDYGNWDQISHQPDAIANLARLYTSLDFVVCHLSFPHVEHSNRLRAELNMW
                     KNFNNIYTDISAIQDIDAPDSFPFPKSEQNVRIAKEVLGAKRIIWGTDSPWSATFNTY
                     EELATWLENVDIFSQEELEDVMYNNAERVYFKEEHVKANRAGIDDTTKELNLY"
     gene            14186..15703
                     /locus_tag="VBC74_000729"
     CDS             14186..15703
                     /locus_tag="VBC74_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEO6941699.1"
                     /translation="MTYYLSIDYGGTNTKALIFDKLGHQIAVSSFETLKNEAQSGHRQ
                     VNLVKTWNAITSAIREVIQISKLSPEQISAVACIGHGKGLYLLDNKLEPLEQGILSTD
                     NRAKDLAQYFESKLDNIWELTRQHIFPSQSPVILRWLKDYQPETYKSIGAVLSAKDFI
                     RYKLTGKVQQEYGDASGNHWINFQTGTYDPAILDFFGIREIENSLPELIDSADLVPGG
                     ISSQAAKETGLVEGTPVVGGLFDIDACALGSGVLESDTFSVISGTWNINTYPSLKPAK
                     QDSGLMTSYFPDRRYLLEASSPTSAGNLDFMLKMLMHQEIDNAKSSGGSIYDNLEEFL
                     THTDATHHGLIFFPFLYGSNTSQDASACFFGLTTKSTKSQMIRAVYEGIAFAHKQHIT
                     DLIKSRGGVPKIIRFSGGATNSPAWMQMFSDILNLPIKTVEGTELGGLGGAILARHAL
                     DKISLKEAVQDMVRVKAIYKPQLSEVKGYEKKYHAYQKLLETLDPIWSELGHLNK"
     gene            15723..16484
                     /locus_tag="VBC74_000730"
     CDS             15723..16484
                     /locus_tag="VBC74_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP endonuclease"
                     /protein_id="HEO6941700.1"
                     /translation="MKQEDIIINSIVFKKQLDKGFTQVQLIKLVKDLGFSRFEIRQEL
                     LQDPDRELPALKAEADFYDINLYYSANEDLIKGGKVNPYLNKGLKEASQLGAPFIKLN
                     VGQTRNLSKEELEPLKEILKSQTIGIKVENNQDPKAATVENCQYFMTLVKELQIPISF
                     VFDTANWAFINQDLYQAVNNLACDTTYLHCKNFIQVAGKPHLSKSLFEGEINLTDLLK
                     SFSNCEYLALEYPTELEILKRDVQRLISISNSQNF"
     gene            16550..18229
                     /locus_tag="VBC74_000731"
     CDS             16550..18229
                     /locus_tag="VBC74_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEO6941701.1"
                     /translation="MIILDKKSYDLLFYLLKLEEPETVMAIANALNQSRRKVYYHLEK
                     INDALPSDVPQIVSYPRVGILLTEKQKAACRLLLDEVTDYSYVMKSSERLQLSLVSIV
                     VAKDRITIDRLMQLNDVSRNTILNDLNELRSELAEKEYNLQLQSTKCRGYFLDGHPLS
                     IIQYLYKLLDDIYHNGSSSFIDLFNHKLSQAFGASTYFSKEVLDYFHHYLFISQRSLG
                     KKINSQDGQFMIQILPFILMAYRKMRLSPEVQTSLNSDFSLVWQRKEYEIAKELADEL
                     EENFQLSLDEIEVGLVAMLMLSFRKDRDNHLESQDYDDMRATLTSFLKELEERYHLHF
                     VHKKDLLRQLLTHCKALLYRKRYGIFSVNPLTEHIKDKYEELFAITSSSVKLLEKAWQ
                     IKLTDDDVAYLTIHLGGELRNSQQSPNKLKLVIVSDEGIAIQKLLLKQCQRYLTNSDI
                     EAVFTTEQYQSVSDLMHVDMVVSTSDALESRFPMLVVHPVLTDDDIIRLIRFSKKGNC
                     ANSNQFTNELEKTIAQYVKEDSERYVLKSKIEKLIHQELLQDVLPLQSTVC"
     gene            18546..19637
                     /gene="ulaG"
                     /locus_tag="VBC74_000732"
     CDS             18546..19637
                     /gene="ulaG"
                     /locus_tag="VBC74_000732"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEO6941702.1"
                     /translation="MPNVKDITRESWILSTFPEWGTWLNEEIEEEVVAEGNFAMWWLG
                     NCGVWIKTPGGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVI
                     DPFAINELDYYLVSHFHSDHIDINTAAAIINNPNLDHVKFVGPYECGEIWKKWGVPEE
                     RIIVIKPGESFEFKDIKVTAVESFDRTCLVTLPVDGAEEHDGELAGLAVTDEEMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDYKIDVAINNYGDNPVGIQDKMTSIDLLR
                     MAENLRAKVIIPVHYDIWSNFMASTDEILQLWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DKDRIEYHHPRGFDDCFEQESNIQFKALL"
     gene            19727..22105
                     /locus_tag="VBC74_000733"
     CDS             19727..22105
                     /locus_tag="VBC74_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEO6941703.1"
                     /translation="MSEFDTKSYLEKLDAWWRAANYISAAQMYLKDNPLLRRELVEND
                     LKVHPIGHWGTVPGQNFIYAHLNRAINKYDLDMFYIEGPGHGGQVMVSNSYLDGSYTE
                     LNPNIEQTEDGFKQLCKIFSFPGGIASHAAPETPGSIHEGGELGYALSHATGAILDNP
                     DVIAATVIGDGEGETGPLMAGWLSNTFINPVNDGAVLPIFYLNGGKIHNPTIFERKTD
                     EELSQFFEGLGWKPIFADVVELSEDHAAAHALFAEKLDQAIQEIKTIQSEARQKPAEE
                     AIQAKFPVLVARIPKGWTGPKAWEGTPIEGGFRAHQVPIPVDAHHMEHVDSLLSWLQS
                     YRPEELFDENGKIVDEIAAISPKGDRRMSMNPITNAGIVKAMDTADWKKFALDINVPG
                     QIMAQDMIEFGKYAADLVDANPDNFRIFGPDETKSNRLQEVFTRTSRQWLGRRKPDYD
                     EALSPAGRVIDSQLSEHQAEGFLEGYVLTGRHGFFASYESFLRVVDSMVTQHFKWLRK
                     SKTHTTWRKNYPALNLIAASTVFQQDHNGYTHQDPGILTHLAEKTPEYIREYLPADTN
                     SLLAVMDKAFKAEDKINLIVTSKHPRPQFYSIAEAEELVAEGYKVIDWASNVSLNQEP
                     DVVFAAAGTEPNLEALAAISILHKAFPELKIRFVNVLDILKLRHPSQDARGLSDEEFN
                     KVFTTDKPVIFAFHGYEDMIRDIFFSRHNHNLHTHGYRENGDITTPFDMRVMSELDRF
                     HLAQDAALASLGNKAQAFSDEMNQMVAYHKDYIREHGDDIPEVQNWKWENIK"
     gene            22482..23705
                     /locus_tag="VBC74_000734"
     CDS             22482..23705
                     /locus_tag="VBC74_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEO6941704.1"
                     /translation="MTNILEVKNLTKIFGKKQKAALEMVKQGKSKTEILEKTGATVGV
                     YDASFEIKEGEIFVIMGLSGSGKSTLVRMLNRLIDPSSGNIYLDGKDIAKMNVEDLRN
                     IRRHDINMVFQNFGLFPHRTILENTEFGLEMRGVSKEERTTLAEKALDNAGLLPFKDQ
                     YPSQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDTNKQTI
                     IFISHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTVLEIDGPQVALTRMHREEVSMLMATNRRRQLLGSLTADAAIEARKKGL
                     PLSEVIDKDVVTVSKDTVITDIMPLIYDSSAPIAVTDDNDRLLGVIIRGRVIEALANV
                     QDETVVESPKETVEA"
     gene            23724..25451
                     /locus_tag="VBC74_000735"
     CDS             23724..25451
                     /locus_tag="VBC74_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEO6941705.1"
                     /translation="MENLLQHKLPVAPFVESTTNWITKTFSGLFDFIQTIGNALMDWM
                     TKTLLFINPLLFIVLITIAVFFLAKKKWQLPTFTFIGLLFIYNQGLWEQLINTFNLVL
                     VASLISIIIGVPLGIWMAKSDKVKQVVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVVFALPPTVRFTNLAIREIPLELIEASDSFGSTAKQKLFKVELPLAKNTIMAGIN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADIGTGFVSGLSLVILAIVLDRVSQFF
                     NSKPGEKQAKTSKVKKWVGLGALALFILAALGRIVVNMTSGNEAKGQKVKIAYVQWDS
                     EVASTNVIAEVLKSKGYDVELTPLDNAVMWQTVANGNADFTTSAWLPKTHGQYFNKYK
                     NSLDDLGPHVENVKIGLVVPKYMNVNSIEELSNQADKQITGIEPGAGIMKSAKQSLKD
                     YPNLSSWKLLSASTGAMTTTLGKAIKNKDQVVITGWSPHWMFAKYDLKYLKDPKKSFG
                     GEEHINTIARKNLKKDMPKVYKIIDKFKWTKEDMESIMLDMDKGMEPAKAAQKWIKNH
                     KKEVSEWTK"
BASE COUNT         8159 a         4227 c         5187 g         8207 t
ORIGIN      
        1 tttgaggaat ttggcaaaaa aaaacatttt ttgctataat aaggtgtcag acaaactcgc
       61 aggtgaaatt cctgccatgt ttttccagaa aggattgggg agtcccaaaa aggctcactc
      121 agtgttaaat aaatatgact tcagttgttg tagtaggtac ccaatggggt gacgaaggta
      181 aaggtaaaat tacagatttt ttatctgctg acgcagaagt aattgcacgt tatcaaggtg
      241 gtgataacgc tgggcatact atcgttattg ataacaaaaa atttaaactt catttaattc
      301 cttcaggaat tttcttcaaa gaaaaaattt ctgtcattgg taatggtgtt gtcgttaatc
      361 cgaaatcatt agttaaagag ttagcttatc ttcacggaga aggtgtaaca acggataacc
      421 ttcgtatttc agatcgtgcg catgttatct tgccatacca catcaagctt gaccaactac
      481 aagaagatgc taaaggtgat aataaaattg gaacaaccat caaaggaatt gggccagctt
      541 acatggacaa ggctgcgcgt gtcggtatcc gtattgctga ccttcttgat agagaagttt
      601 ttgctgagcg tttgaaaatt aatttagctg agaaaaatcg tttatttgaa aaaatgtatg
      661 acagtacacc tcttgaattt gatgatattt tcgaagaata ttatgaatat ggtcaacaaa
      721 ttaagcaata tgtgactgat acatccgtta tcttaaatga tgctcttgat gccggtaaac
      781 gtgttctttt tgaaggagca caaggagtta tgctcgacat tgatcaagga acatacccat
      841 ttgtaacatc ttccaatcca gtagcaggtg gtgtcacaat tggttcggga gttggaccaa
      901 gtaaaattaa taaagtggta ggtgtatgta aagcttacac tagccgtgtt ggcgatggac
      961 cattcccaac agaacttttt gatgaggttg gtgaccgtat tcgtgagatt ggtaaagagt
     1021 atggtacaac gactggtcgt cctcgtcgcg ttggatggtt tgattctgtt gttatgcgtc
     1081 acagccgtcg agtatcaggt attactaacc tctctctgaa ttcaattgat gttctttcag
     1141 ggcttgatac ggtgaaaatt tgtgtggctt atgaccttga tgggaaacgt attgactatt
     1201 acccagcaag ccttgaacag ctaaaacgtt gtaaaccaat ctatgaagaa ttaccgggct
     1261 ggtctgaaga tattacagct tgccgtagct tagatgatct tccagaaaat gcacgtaatt
     1321 acgttcgccg tgttggcgaa ttggttggtg ttcgtatttc tactttctca gtagggcctg
     1381 gtcgtgaaca aacaaacatt cttgagtctg tttggtcaaa tatttaatat taaaaatctg
     1441 actgtacagt cagacttttt gatgtttact ttttcttttt aatagataga tactaataag
     1501 acttaaggac ttcccagaaa tcaataaaac tactataatt gataaaaaaa gaagtgaatt
     1561 gatattgtga atcaaagtat gtaataatac tagcgataca actgttagaa tactaccagt
     1621 catcaataag cgttccagag gatagtagcc atgttgctta tgcatttcta attttaatag
     1681 tagaccagct gacaattctg ctaggaagca aataggtaga gctatcaaat taataagaat
     1741 catagaccaa ccaaatcctt gaccataacc tatatatgat agactatttt tgggaattgt
     1801 tgacgtgtga attataaaat caatcgtaaa gataatgtct ataagaagta gtagcgctaa
     1861 cagataatgt agtaagaaga ttgttcttaa ccgtttatta atcaatattt catttcctcc
     1921 tacttattta ccaataagct tattatatca taatgtataa tcaaacattc gttagtactg
     1981 attaaaaggt ttatggaggg gcggttacta ttgtttcatt gaattagtaa ctttaacgac
     2041 gaaatgttat aatgttatta gtttataaaa gaggagattg tatgcgtaaa tttttccaag
     2101 atgttattgt agaggtggct tattatttag agattgcttt gtcagttatt ctggcaacag
     2161 ctattatttt ctttagttta gcactctttc atgatttgtt tagcatgttg tctgttgatc
     2221 aaaatgttga taagttgttc cagactttcc ttgctcgcgc catgacaata gcagtcggga
     2281 ttgagttgat caaaatgttt tcaaaacctt cccctgatac tttaattgaa gtgttattgt
     2341 ttgccttagc tcgtcaactt gtaattgaac atggtagtgt ttcagattac cttatgggga
     2401 ttgcttcagt tgctattttg tttgctgttc gtaaatatct tttcacccat tttgatgagt
     2461 caagcaacat tgttttacgt gctaataaca aagtcaaaaa agctaatatt ttagctaggg
     2521 ttcaactacc ttgtcaaccc aatgaaactt taggagactt acttgttcgt catttaaatg
     2581 atgaggaaaa ggacgttgct attggttcag tagtctatat taataacgtt gcattgcgtg
     2641 ttgatagtat gcacgatggt cttgtgacgc gtgtagaaat cattaggagt ctacattaaa
     2701 aaatataaaa aatcatgtat cttaggatac atgatttttt tgtaaattag gttcagcaca
     2761 attttgcaca cttaagtgtg tacttcttgg tcttttttaa aacgcttaca aaaaatataa
     2821 taaagataca aaaaaacatt acttaaataa agagaggtct ttagttatgg aaaacttcct
     2881 agctcccctt aattggttct cgcaaaatat cttgcagaat ccagccttct tcgtaggtct
     2941 gttggtactc attgggtatc ttttattgaa gaaaccactt catgatgtct ttgctggttt
     3001 tatcaaagcg accgttggat atttgatttt gaacgtaggt gcaggaggat tggttaatac
     3061 attccgtcca atcttagttg ctcttgctaa aaaatttcat ttagaagctg ctgttattga
     3121 cccttacttt ggtttagcat cagcaaatgc taagttagaa acaatgggct ttatcagtgt
     3181 tgcaacaaca gcactcttga ttggttttgg gattaacatt ctcttggttg ctcttcgtaa
     3241 agtaactaaa gtccgtactc tttttattac tggtcatatc atggtacaac aagctgcaac
     3301 aatttcagta tttgtactgc tcttgattcc acaacttcgt aatggttttg gtgcatgggc
     3361 tgttggtatc atttgtggtc tatattgggc ggtaagttct aatatgaccg ttgaagcaac
     3421 tcaacgcctt acaggtggtg gcggatttgc catcggacat caacaacaat ttgcaatttg
     3481 gtttgttgat aaagttgcgc catttttcgg taaaaaagaa gaaaatcttg ataacttaaa
     3541 attaccaaca ttcttaaata ttttccatga tacagttgtt gcatcagcaa cattgatgct
     3601 agttttcttt ggaggaatct tagctgtcct tggtccagac attatgtcta atgttaaatt
     3661 aatcggacct ggtgcatttg taccaactaa gcaagctttc ttcatgtaca tccttcaaac
     3721 atcattgaca ttctcagttt accttttcat tttgatgcaa ggtgtacgca tgttcgttac
     3781 agaattgaca aatgccttcc aaggtatctc aaacaaactt cttccaggat cattcccagc
     3841 ggttgacgtt gcagcttcat atggttttgg ttcttcaaat gcagttcttt caggatttgc
     3901 ttttggtttg attggtcaat taattacaat cgctctactt gttgtcttca aaaatccaat
     3961 cttgattatc acaggattcg ttccagtatt ctttgataat gcggcaatcg ctgtttatgc
     4021 agataaacgt ggtggctgga aagcagcagt agctctatca tttatctcag gtattattca
     4081 ggtggcactt ggtgctgttg ctgtaggtct tttaggactt gcaggtggtt atcatggaaa
     4141 tatcgacttt gaattcccat ggttagcatt tggttacatc ttcaagtatt taggtattgc
     4201 tggttacgtg attgtatgtc tcttcttctt agctattcca caacttcaat ttatgaaatc
     4261 taaagataaa gaagcatact atcgtggtga tgcgcctgca gaataataga aatttataac
     4321 ggaggaaata aaaatggtta aagttcttac agcatgcgga aacggcatgg gatcatcaat
     4381 ggttatcaaa atgaaagttg aaaatgcgct tcgtcaatta ggcgttagca actttgagtc
     4441 agcatcttgc tcagtcggag aagcaaaagg tttagctgcg aactatgata tcgtggttgc
     4501 ttcaaaccac cttatccatg aattagacgg acgtacaaaa ggtcatttag taggtcttga
     4561 caaccttatg gatgacaatg aaataaaaac aaaactacaa gaaattttgt aagttaatag
     4621 acaaagggag tgagacaaaa gtttctctcc catatcttta gacttttaag gagaaatcat
     4681 gaatttaaaa caagccttta tcgaaaatga ctcaattcgt cttaaattat ctgcaagcga
     4741 ttggaaagaa gcaatcaaat tatcaattga tccattaatt gaaagtggtg ctgttgatgc
     4801 agaatattac gatgccatta ttgaatctac agaggaattt ggtccgtact acattcttat
     4861 gccgggtatg gcgatgcctc atgctcgtcc agaagcaggc gtaaaacgtg atgcattttc
     4921 attaattaca ttaacagagc cagtagtctt tccagatggc aaagaagtct cagtattgtt
     4981 agcacttgct gcaacaagtt cagctattca tacttctgtt gctatccctc aaattattgc
     5041 cctttttgaa ttggaaaatt caattcagcg cttgacggaa tgccaagaag caaaagaagt
     5101 ccttgcaatg gtagaggaat caaaaaacag cccatattta gaaggtttag atttagaaag
     5161 ctaatcaata aactgatatt aatagaaagg ataggactta taaaaaatct ctgtgataag
     5221 aaactctttg ccacaaataa agtgagtatt tttatcaggt ccttagaata aacaacatga
     5281 caaaacgttt accaaattta caagttgcac ttgaccactc agatttacaa ggagctatca
     5341 aagctgccgt atcagtagga catgaagttg atgttattga agctggaact gtttgccttc
     5401 ttcaagtagg tagtgaatta gttgaggtcc ttcgcagtct cttccctgac aaaataattg
     5461 ttgctgatac aaaatgtgct gatgctggtg ggactgttgc taaaaataat gctgttcgcg
     5521 gtgcagactg gatgacttgt atctgttgtg ctactattcc aacaatggaa gcagccttaa
     5581 aagctatcaa agaagaacgc ggcgatcgtg gagaaattca aatcgaacta tatggtgatt
     5641 ggacttatga gcaagcgcaa caatggttgg atgcaggtat ttcacaagcc atttatcacc
     5701 aatctcgtga tgcgcttctt gctggcgaaa catggggaga aaaagatctt aataaggtta
     5761 aaaaattgat tgatatgggc ttccgagttt ctgtaactgg tggtttatca actgatactc
     5821 ttcaattatt tgaaggtgtt gatgtcttta cttttattgc gggtcgtggt attacagagg
     5881 cagatgatcc agccgcagca gcacgtgcat ttaaggatga aattaaacgt atttgggggt
     5941 aatgtatgac acgtccaata ggaatttacg agaaggcaac accaaaacat tttaactggt
     6001 tagaacgcct acaatttgct aaagaattgg gatttgattt tgtagaatta tccattgatg
     6061 agtcagatga acgtttggca cgtttggagt ggtcaaaaga agaacgcctt gagttggtca
     6121 aagctatttt tgagacagga gttcgtgttc caacaattac atttagtggt catcgtcgtt
     6181 tcccaatggg atctaatgat cctgagaaag aagcgcgtgc gatggatatg atgaaaaaat
     6241 gtatcgtctt tgctcaagat attggtattc gtaatattca attagcagga tatgatgtgt
     6301 actatgaaga aaaatctcca gagacacgtg cacgctttat taagaatctt cgtcaagctt
     6361 gtacttgggc cgaagaagca caagttattc tgtctattga gattatggat gacccattta
     6421 tgaattccat tgagaaatat ttggctgtgg aaaaagagat tgattctcct tatctctttg
     6481 tctacccaga tactggaaat gtatcagcat ggcataatga tctttggagt gaattttata
     6541 atggacatcg ctcaattgca gcgcttcata ttaaagatac ctatgctgtt accgaaacgt
     6601 ctaaaggtca attccgtgat gttccatttg gtcaagggtg tgttgattgg gaagaaatgt
     6661 ttgctgtcat taagaaaaca aattataatg gtccattctt gattgaaatg tggtcggaga
     6721 actgtgagac tgtagaggaa acacgtgcag ccattaaaga ggcgcaagat tttctttacc
     6781 cattaatgga gaaagcagga gtgaaataat atggcaaaat cattgcaaga aatgcgtgaa
     6841 cgcgtgtgtg aagctaataa atcactacca gttcatggtt tagttaaatt tacttggggt
     6901 aacgtttctg aagttgaccg cgaagcagga ttgattgtta tcaaaccttc aggagttgac
     6961 tatgaccaat tgacacctga aaatatggtt gtcacagact tagagggaaa tatagtggaa
     7021 ggggatttga atccatcatc tgatcttcca actcatgttc aactttataa agcatggcca
     7081 gaagtcggtg gtattgttca tactcattca acagaagcag ttggctgggc acaagcgggt
     7141 cgcgatattc cattttatgg aacaacacat gcagattatt tttatgggcc agtaccatgt
     7201 gctaggtcac ttagtgaaga tgaagttaac actgcttatg aaaaagaaac aggtagtgtt
     7261 attatagagg aatttgaacg tcggaattta gatccaatgg ctgttcctgg tattgttgta
     7321 cgtaatcatg gaccatttac ttgggggaaa gatccagcac aagcagttta tcactcagtc
     7381 gttcttgaag aagttgccaa gatgaatcgt ttcactgaac aaattaatcc acgtgttgaa
     7441 ccagctccaa aatatattat ggataaacac tatttacgta agcatggacc aaatgcttat
     7501 tatggtcaaa aaggacaaga acattaagga gaataatatg aaatactttt tagatactgc
     7561 cgatgtttct gaaattcgtc gtcttaatcg tttaggaatc gttgatggtg ttactaccaa
     7621 tcctactatt atttcacgtg aggggcgtga ctttaaagaa gttattaacg aaatttgcca
     7681 aatcgttgat ggtccagtta gtgcagaagt gacaggttta acttgtgatg aaatggttac
     7741 cgaagctaga gaaatcgcta agtggtcacc aaatgttgtt gttaaaattc caatgactga
     7801 agaaggttta gcagcagttt cacaattatc aaaagaaggt ataaagacaa atgtgacatt
     7861 aatatttact gtggctcaag ggctttcggc aatgaaagct ggtgcaacct ttattagtcc
     7921 ttttgtgggt cgtcttgaag atattggaac agatgcttat gctttgattc gtgaccttcg
     7981 tcacattatt gatttttatg gtttccaatc tgagattatt gctgctagca ttcgtggatt
     8041 ggcgcatgtg gagggcgtcg ctaaatgcgg tgcgcacatt gccactattc cagataaaac
     8101 atttgcatca ctatttactc acccgcttac tgataaaggg attgaaactt tcttaaaaga
     8161 ttgggatagt ttcaagaaga agtagtgtta cgataaagca aactcttaat tatactagtg
     8221 aaagacaaac tttgataatg ggtttgtctt ttttataatc ttctccaata agaacactta
     8281 ttttcatttt tcttattaaa tgatataata cggataaatt aagtctaatt agaataagga
     8341 aatagagatg tttgagaagg taaccattag tgatattgca aatttagtag gtgtgtcaaa
     8401 agctacagtt tcctattatc tcaatgggaa ctataagaaa atgtctctgc aaacgaaaga
     8461 aaaaatccgt ttagctatta aggagactgg ttatcaacca agtaagattg ctcaaagttt
     8521 agttacaaaa aatacccgta ctattggggt cgttattgct gatattacca atccatttat
     8581 ttcgtctgtg atgaagggaa tacatgatac ttgtcaacaa tttggttact cggttaactt
     8641 tactaactcg gataatgata ttgacattga gttggaaaat ttaaaccgtt taaatcagca
     8701 aaacgtttca ggaattattt tagattctgt agatcctaat cattcattta tagaaacttt
     8761 gagtaatgat cgtcttgtca tggttgatag acaagctaaa gatattaagg tagatactgt
     8821 agtatccgac aataaagaat cgactcaaat atttcttgaa aaaatgcaag aaactgggta
     8881 tcatgacatc tattttgtca cttatcccat tgaagggatt tcaacccgtg agcttcgtta
     8941 cgaaggtttt aaagaagtgg tcagttcaaa tcctgataag ttgattatta tcactgaaga
     9001 tggtagcaca cagagaattc ttgatattat tgaacattca gagcaaaaac cagggttctt
     9061 aatgatgaac ggtccaactc ttttgaattt tatgaaaaaa ttaaatcaat caacggtcag
     9121 ctaccctgag gattatggtc ttgggagtta cgaagattta gaatggatgc aggtgcttac
     9181 accaaacgtt tcctgtataa aacaggattc atatggcatt ggttgtctgg ctgcccaatg
     9241 tttaattgaa aaaattagtc aaggaaacga accaactact gcaaggttac tagaagtaaa
     9301 aaatcaaatc gttattcgtc aatcttttta aaaatgtttg cgagagcaag cattttttta
     9361 taaaaaagca aaaaacacta tgtaaacgct tgcttttata aaaaatgata gtataataga
     9421 actataaatg agttaaccgt ttaacttaag aataggtaag cttttaacga accatctcta
     9481 actctcttaa ctcaaatgtt tttatttttt tgcaccatta gttaaacggt taactcaagt
     9541 tttatcttca ccaaaggagg atagaatgaa gattaataaa ttattgtcag acagacttga
     9601 agcttgttat tcagtttgtc gtttgcaaaa tcaagactat gattttttat tagtagcttc
     9661 agaggttgat caagcctgct ttgcttatga tttgaatagc gatttagcaa aaattgttgt
     9721 ttgggaagat gtgggaggta ccatgagtat tattccaatt cccggaacca tggatttttt
     9781 ggcaacccag aaattttacc cagggtttaa ggccaaagat tgtcaggttg tttatgcaag
     9841 ttttcaagga gatcattggg acatttgtaa cgcatttgat tttccgtatt tacatcgctt
     9901 tgatctcatt aaaggtaaag aaaatcaact ttactttata ggttgtacaa ttgctaacag
     9961 taaagcctac actgaggatt ggagtgataa aggccgaatt tttgttgctc gttttaatac
    10021 tcaaaaccat acattggaag gattgcaaca attgcctcaa actttattaa aaaatcatgg
    10081 atactatgcc attcaggatg aaggatattc attgattact tcagtagaag gggtactcaa
    10141 actcacttat ccagaatttt ctactacagg cgactggcaa ttagaacggc ttttcgatga
    10201 ggagacaagc gatgtggtga aagtggatat taatcgggat ggtaaggatg agtatgtgat
    10261 catccaaggt tttcatggag atcgtttacg tatcttcact gaagatttcg gtcgagaatt
    10321 attccattat cctgaaaaaa ccccatttgg tcacgctatt tggagtggtc gtttacttaa
    10381 tcagacttgt ttcgtattcg ggtggcgatc agaaaaagca gaattaaggc tttttcactt
    10441 tgtagatggg cacttggttt cagaattagt agatgcaaaa gcagcttcta gtaatgtctt
    10501 agcttttgaa aaagatggaa aagcttatct tttctcagcc aataacggac gtggcgaagt
    10561 tgctctttat caattagtaa aataaattta agaaggagac aatatggaca agaaaaaaat
    10621 cttagtaacg ggtattgtgc ctaaagaagg tctaagaaag cttatggacc gatttgatgt
    10681 tacttattca gaagatcgcc cattttcacg tgactatgtg ttagagcatt tatctgaata
    10741 tgacggatgg ttactcatgg gacaaaaagg tgataaagag atgattgatg caggtgaaaa
    10801 cttacaaatt atttctttaa atgcggttgg ttttgatcat gtagatactg cgtatgctaa
    10861 agaaaaagga attgtagtct ctaattcccc tcaagcagta cgagttccga cagcggaaat
    10921 gacttttgct ttaattttag ctgccagtaa gcgccttgca ttttatgata gtattgtaag
    10981 aagtggagag tggattgatc cgagcgaaca acgttatcaa ggtttgactt tgcaaggttc
    11041 aacattaggt atttacggaa tgggacgcat cggtttgaca gtcgcaaatt ttgctaaagc
    11101 atttgggatg actgttgttt acaatgatgt ttatcgactt cctgaagata aggaaaaaga
    11161 gcttggtgta acctatctcg aatttgatca gcttattgaa acagctgatg tcataaccat
    11221 ccacgcccca gcattaccat caacaatcca caaattcaat aaagatgttt ttgctaagat
    11281 gaaaaatcga agctacttaa tcaacgctgc tagaggccca atcgtttctg aagaagcact
    11341 tattgaagct ctgaaagaag gtgaaatcgc gggtgcggga ttagatgttt ttgagaatga
    11401 accacaggta tctgaaggct tgagaagtct tgataatgtt atcatgagtc cacatgctgg
    11461 gacaggtact attgagggac gtagaacttt ggctgaagaa gcagctgaca acatcattgc
    11521 cttctttgat ggcaagccac aaaatattgt caataagtaa ttggctagtg agtgcagagc
    11581 catcttttga aaaaatggga cctcccattt ttttacgggt aaatgtaagc gcttaataat
    11641 ttaataaagg tgaacttatg ctagaagctt tacataaatt ctttgaagtt tttggtgcag
    11701 caactgttgt acccgctatg atttttattg tcagcctttt tctaaaggtc aatccaaggc
    11761 gagctttctt tagcgcctta cgagctggtg ttggactcac tggctttggt tggattatat
    11821 cagcttttgc tcctatggtg actaagtata tccagcagat ggttaaaact actggtctcc
    11881 atttaccaat tgtagatatt ggttggcagg ctggttctct caccgccttt tcttctgaga
    11941 ttggcctctc tttctttgtt tttggcttgc taattgaatt ggggcttttc ttgcttggga
    12001 ttacccgagt atttgtccca tctaatcttt ggaataactt tggctatatg atttggggta
    12061 caatggccta tgcagcaaca ggtaatttta tattgtcttt cgcatttatg gtttttgtct
    12121 tactctattc attagtgatg tcagaagtat tggcagatcg atggtcggag tattacggtg
    12181 ttaaaaatgc aacgatcaac tcgattcata atattgaaac ccttattcca gctcttattc
    12241 ttgatcctct ttggaaccta cttggtgtca ataaggtaaa gttgaatcca gaatcattaa
    12301 agactaagtt agggatattt ggtgagccaa tgacacttgg ttttatactt ggtgtgatta
    12361 ttggtgtatt aggtagttta cgtaaccttg caagtattga tacttgggga ggtatacttg
    12421 gttttgcagt tgctcttgca gcggttatga ctatttttcc tttgattacg ggggtatttg
    12481 ctagtgcttt tgctcctctt gctgaagcag ttgaacgcaa taagaaaaaa gaaagtcaag
    12541 ctgagcaagg agcactggat aaaaaacgtt ggtttattgc agtagatgac ggtgttggtt
    12601 ttggagaacc agcaactatt atcgcaggtc tcattttagt accaataatg gttgttattt
    12661 ccttgatttt acctggaaat gaagctttac cagtcgttga ccttattgct attcctttta
    12721 tgattgaagc tatgatagct gtgtcaaaag gaaatatcct taaagctatt ttgaatggaa
    12781 tcatatggtt ttcactaggt ctttatgctg ctagtgccct aggtccaatc tacacagaag
    12841 ctgttaagca ttttggaact gctttaccag caggggtaac tttgattatg agctttaatc
    12901 tcctagctca tcctttgaca gctctagtct tctttgcttg gatatctggg aatccactct
    12961 atattggtgt gacagttatt atctatcttg ctttcttgtt cttgcttcgt actcgtcgtg
    13021 aatctattta cgcatacctt aaaactatgg ctgataagaa tagcggatat gaaggtagtt
    13081 gtgaaaaaat ctttattgct gaagattaaa aatctgttgg aggaatactc atgaaaaaaa
    13141 ttgatggaca tgctcatatt gttgatacaa ttgcaggatt taatggtaaa gggcgtctta
    13201 atgcccttgg aaacggttat gctatttggg atgatggtga tttaattaag ctcatcccag
    13261 acgaatacgg tgacaaagca tttacagctg aatctttttt gaagtatatg gatgctaatg
    13321 gtgtagataa agcagtcatt ttgcaaggtc atcttaatgg ttatcaaaat tattatacgc
    13381 atttagctat taaacgttat ccagaacgtt ttacaggcgc attctctgtt gatccatttg
    13441 cagataatgc aatgcaaatt gtaaaacgtc atgttgaagt ccttggtttt cgagctatta
    13501 aatttgaaat tagtcaagga gggggtatcc atggataccg tggacaaaaa acgcctttcc
    13561 gtcttgatac agacccacat gtgagtcgta ttttgactta tctgttagac tatccaggat
    13621 ttgtagttac agttgattat ggcaattggg atcaaattag tcaccaacct gatgctattg
    13681 ctaatttagc tcgcctttat acgagtctcg attttgttgt ttgtcacctc tcttttccac
    13741 atgttgagca ttcaaatcga ttgcgtgctg aacttaatat gtggaaaaac ttcaacaata
    13801 tctatactga tatttcagct attcaagata ttgatgcccc agatagtttt ccttttccaa
    13861 aatcagaaca aaatgtacgt attgctaaag aagttcttgg ggctaagcgt atcatttggg
    13921 gaactgattc tccatggtca gcgaccttca atacttacga agaattagct acttggctag
    13981 agaatgttga tattttcagt caagaggaac tagaagatgt tatgtacaat aatgctgagc
    14041 gtgtttactt taaagaagaa catgtaaagg ctaacagagc aggtatagat gatacaacca
    14101 aagagctcaa tctttattag aagagttttc aaggtacaag agtttcttgt acctcctttt
    14161 taaatcaaca atgaggagac aaattatgac ttactacctt agcattgact acggcggtac
    14221 aaataccaag gcgcttattt ttgacaaatt aggacaccaa atcgctgttt cgagttttga
    14281 aactttaaaa aatgaggctc aatctggtca tcgtcaagta aaccttgtta aaacgtggaa
    14341 tgctataact tctgctatta gagaggttat tcaaatctca aaactcagcc ctgagcagat
    14401 tagtgcagta gcatgtattg gacatgggaa aggtctttat ctgctagata ataagttgga
    14461 gccacttgaa caaggaattt tgtctacaga taatcgtgcc aaagatttgg cgcaatattt
    14521 cgaatctaaa cttgataata tttgggagtt gactcggcaa catattttcc cttcacaaag
    14581 tccagttatt ttacgttggc ttaaagatta tcagcccgaa acctataaat caataggggc
    14641 ggtcctttct gcaaaggact ttattcggta taagcttaca ggaaaagtac agcaagaata
    14701 tggtgacgct tcaggtaatc attggataaa ttttcaaaca ggaacttatg atccagctat
    14761 tttagatttt tttggcatta gagagataga aaactcactt cctgaactta tagatagtgc
    14821 agatttagtt cctgggggaa ttagttctca agcagcaaaa gagactggtc ttgtagaagg
    14881 aacccccgtt gttggagggc tctttgacat cgatgcttgt gctcttggat caggtgtttt
    14941 agagtcagat acttttagtg ttatttcggg aacttggaat attaatactt atccaagttt
    15001 aaaaccagca aagcaagata gtggtcttat gacttcctat tttccagatc gtcgttatct
    15061 cttagaggca agtagcccta cttctgcagg gaatcttgat tttatgttaa aaatgctcat
    15121 gcatcaagaa attgataacg ctaaatctag tggaggttct atctatgata atttagaaga
    15181 atttctcact catactgatg ccacacatca tggacttatt ttctttccgt ttctttacgg
    15241 tagtaacaca tcacaagatg ctagcgcttg cttttttggg ctaacaacta aatcgacgaa
    15301 atctcagatg atacgtgcgg tatatgaagg tattgcgttt gcacataagc agcatatcac
    15361 tgatttaata aaaagtaggg gcggtgtgcc aaaaataatt cgtttctctg gcggagctac
    15421 caactcacca gcatggatgc aaatgttttc tgatatctta aaccttccta ttaaaacagt
    15481 agaaggcaca gaattaggag gattaggagg agctatttta gcacgtcatg ctttagataa
    15541 gatttcgtta aaggaagcag tccaagatat ggttcgtgta aaagctattt ataaacctca
    15601 attatccgaa gtaaaggggt acgaaaaaaa atatcacgcc taccaaaaat tattggaaac
    15661 actggatcct atttggtcgg aactcggtca tctgaataag tagggatatc aaaggagttg
    15721 ttatgaaaca agaagatatt attatcaata gtattgtatt caaaaaacag ttagacaaag
    15781 gttttacaca agttcagctc attaagttag taaaggattt aggattttca cgatttgaga
    15841 tacgtcaaga attgcttcaa gatccagata gagagctacc cgctttaaaa gcagaggctg
    15901 atttttatga tatcaatctt tactatagtg ctaatgaaga ccttatcaaa ggagggaaag
    15961 ttaatcctta tcttaataaa ggccttaaag aagcatcaca gttaggagct cctttcatta
    16021 agctaaatgt tggacaaacc agaaacttaa gtaaagaaga gttagaacct cttaaagaga
    16081 tattgaaaag ccaaactatt ggtataaagg tggaaaataa tcaagaccct aaagcagcta
    16141 cagtggagaa ttgccaatat tttatgactc tagtgaaaga attacaaata ccaataagtt
    16201 ttgtttttga tactgctaat tgggccttta tcaaccaaga cctataccaa gcggtaaata
    16261 acttagcttg tgataccacc taccttcact gtaaaaattt tatacaggta gctggaaagc
    16321 cccacctctc gaagagcctc ttcgaaggag agattaatct gactgatttg ttaaagagtt
    16381 tctctaattg tgagtatcta gctctagaat accccacaga gttggagata ttaaaaaggg
    16441 atgttcaacg tttaatctct atcagtaata gtcaaaactt ttaatgaaaa ggctttaaaa
    16501 agatgttata ataagtctgt atgaattcga gtagaaaaga ggtatattct tgattattct
    16561 cgataaaaaa agttatgacc tcctctttta cctattgaaa ttagaggaac ctgaaacagt
    16621 tatggcaatt gccaacgcac ttaatcagtc tagacgtaaa gtgtattatc acttagagaa
    16681 gataaacgat gcactgccta gcgatgtgcc tcagattgtt agttatccac gtgtaggaat
    16741 cttgctaaca gaaaaacaaa aagcagcctg tcgtctttta ttagatgaag tgactgatta
    16801 tagttacgtc atgaaaagta gtgagaggtt gcagctgtct ttagtatcta tcgtagtagc
    16861 taaggaccgc ataacgattg ataggttgat gcaactaaac gatgtttctc gaaatactat
    16921 cttaaacgat ttaaacgaat taagaagtga gttagcagag aaagaatata atttacagtt
    16981 acaatcaaca aaatgtcgtg gttatttttt agatggtcat ccattgtcca ttatccagta
    17041 cttatataag ctcttagatg atatctacca taatggaagt agtagtttta tagacctttt
    17101 taatcataaa ctgtctcaag cttttggtgc cagcacttat ttttctaaag aggttcttga
    17161 ttattttcat cattatctct tcatttctca acgaagtcta ggtaagaaaa tcaacagtca
    17221 agatggtcag tttatgattc agattttgcc ttttatacta atggcttatc gtaagatgcg
    17281 attaagtcct gaagtacaga cctctcttaa tagtgatttt agcttggttt ggcaacgtaa
    17341 ggaatatgag attgctaaag agttggctga tgagctggaa gaaaattttc agttatcact
    17401 ggatgagatt gaagtgggac tagtagccat gcttatgctt agtttccgca aggaccgtga
    17461 caatcattta gagagccagg attatgatga tatgcgagct actctaacca gttttttgaa
    17521 agaattggaa gaacgatatc accttcactt tgttcataaa aaggacttac taagacaact
    17581 tcttactcac tgcaaggcac tcttatatcg taaacgttat ggtatttttt ctgttaatcc
    17641 tttaacagag catattaaag acaaatatga agaacttttt gccataacct cgtcttctgt
    17701 aaagctttta gagaaagctt ggcaaatcaa attgaccgat gatgatgtag catatctaac
    17761 gattcattta ggaggggaac ttcgtaatag tcaacaatct cctaataaac ttaagttagt
    17821 tattgtatct gatgaaggaa tagcgattca gaaacttctt ttaaagcaat gtcaacgcta
    17881 cttaacaaat agtgatatag aagctgtttt tacaaccgaa cagtatcaaa gtgtgagtga
    17941 tcttatgcat gtagatatgg ttgtctctac tagtgacgct ttagaatctc gttttccgat
    18001 gttagtagtt caccctgttt tgacagatga tgatattatt cgcttgattc gcttttctaa
    18061 aaaaggtaac tgtgcaaata gtaatcaatt taccaatgaa cttgaaaaaa caattgctca
    18121 atatgtcaag gaagatagtg aacgctacgt gctgaaatct aagattgaga aacttattca
    18181 tcaagaattg ctccaagacg tccttcccct tcaaagtaca gtttgttaaa atataatttt
    18241 atggtagcct ctcagctttt ctgaagacag aaaaagaagc tggggctttt ttggtgcttt
    18301 taaacaatgt gctagatatt taatcgcaat gagttcctct atgacatgat aattaaagtt
    18361 gagaaagcta gtacttttaa tgggagtact ttttatttgt gcttatttta attaaaatta
    18421 tgagtctaat ttggaacaga cagcccagtt ttgaacacat aaatgtgtga cacttggtct
    18481 ttttttcaaa agtatttgaa cttatactag tagatgtaag aaacaattaa tcaggaggaa
    18541 ataaaatgcc taacgttaaa gatattacta gagaatcatg gattttatca acattcccag
    18601 aatggggtac atggctaaac gaagaaattg aagaagaagt tgttgctgag ggcaactttg
    18661 caatgtggtg gttaggaaac tgtggtgttt ggattaaaac accaggtggt gctaatgttg
    18721 ttatggacct atggtcaaac cgcggtaaat caacaaaaaa agtaaaagat atggttcgtg
    18781 gacaccaaat ggctaatatg gcaggtgttc gtaaactcca accaaactta cgcgcacaac
    18841 caatggttat tgatccattt gctattaatg aattggatta ctatcttgtt tcacatttcc
    18901 acagtgacca cattgatatc aacactgcgg cagccattat taataatcct aacttagacc
    18961 atgttaaatt tgttgggcca tatgaatgtg gtgaaatttg gaaaaaatgg ggagttccag
    19021 aagaacgaat catcgtgatt aaaccaggtg aatcatttga gtttaaagat atcaaagtta
    19081 ctgctgttga atcatttgac cgtacttgct tggtgacact tccagtagat ggtgcagaag
    19141 aacatgatgg agaacttgct ggtttagccg tgactgatga agaaatggct cgtaaagcag
    19201 ttaactatat ttttgaaaca ccaggtggaa caatctatca tggagctgac tcacattttt
    19261 caaattattt tgctaaacat ggtaaagact acaagattga cgtagctatt aacaactatg
    19321 gtgataaccc agttggtatt caggataaaa tgacttcaat cgatcttctt cgtatggcag
    19381 aaaatctccg tgccaaagtc attattcctg tacactacga tatctggtct aacttcatgg
    19441 cttctacaga cgaaatctta caattgtgga aaatgcgtaa agaacgcctt caatatgact
    19501 tccatccatt tatttgggaa gttggaggta aatacactta tccacaagat aaagatagaa
    19561 ttgaatacca tcacccacgt ggttttgatg attgcttcga acaagaatca aatattcaat
    19621 ttaaagcact tctataatgg ctaatgagag actgtatgtc gcatgatatg cagtttttca
    19681 attttaaaat atgttactaa aatcacaatt aagattagga gaaataatgt cagaatttga
    19741 tacaaaatca tatttagaaa aacttgatgc atggtggaga gcagctaatt atatttctgc
    19801 agcacaaatg tatctaaagg ataaccctct cttgagacga gagctggttg aaaacgactt
    19861 aaaggttcat ccaattggtc actggggcac tgtacctgga caaaatttta tctatgctca
    19921 cttaaatcgc gctattaata aatacgattt agacatgttc tatattgaag gtccaggtca
    19981 tggtggtcaa gttatggtat ctaattcata tttagatggt tcatatactg aattaaaccc
    20041 aaatattgag caaacagagg atggctttaa gcagttatgt aaaatcttct ctttcccagg
    20101 tggaattgca tcccatgctg caccagaaac accagggtca attcatgaag gcggtgaact
    20161 tggttacgca ctttctcatg cgacaggtgc catcctagac aatccagatg ttattgctgc
    20221 gactgttatt ggtgatggtg aaggtgaaac aggaccactt atggctggtt ggctatctaa
    20281 tacctttatc aatccagtaa atgatggtgc tgttctacca atcttctatt taaatggtgg
    20341 taaaatccat aacccaacta tctttgaacg taagacagat gaagaattat ctcagttttt
    20401 tgaaggatta ggttggaaac ctatttttgc agatgttgtt gaactctctg aggatcatgc
    20461 ggctgctcat gctttgtttg cagaaaaatt agatcaagct attcaagaga ttaaaaccat
    20521 tcaatcagaa gcacgacaaa aaccagcaga agaagctatc caagcaaaat tccctgtctt
    20581 agttgcacgt attcctaagg gttggactgg tccaaaagct tgggaaggaa caccaattga
    20641 aggtggcttc cgcgctcacc aagtaccaat tccagtagat gcccaccata tggaacatgt
    20701 tgattctctt ttgtcatggc ttcaatcata ccgtccagaa gaattatttg atgaaaatgg
    20761 taaaatcgtt gatgagattg ctgctatttc accaaaaggt gatcgtcgca tgtccatgaa
    20821 tccaataacc aatgctggta ttgttaaagc aatggataca gcagattgga agaaatttgc
    20881 tcttgatatt aatgttccag gtcaaattat ggcacaagat atgattgaat ttggaaaata
    20941 tgcagcagat ttggtagatg ctaatccaga taatttccgt atttttggtc cagatgaaac
    21001 gaaatcaaat cgtcttcaag aagtgtttac acgtactagc cgtcaatggc ttggtcgtcg
    21061 taaaccagat tatgacgaag ctttaagtcc agctggacgt gttattgatt cccaattgtc
    21121 tgaacaccaa gcagaaggtt tcttagaagg ttatgtttta actggtcgtc acggcttctt
    21181 tgcttcatac gaatcattcc ttcgcgttgt agattcaatg gtaacacaac atttcaaatg
    21241 gttacgtaaa tcaaaaacac acacaacatg gcgtaaaaat tatccagcgc ttaacctaat
    21301 tgcagcttca actgttttcc aacaagatca taatggttat actcaccaag atccaggtat
    21361 tttaactcat ttagctgaaa aaacacctga atacattcgt gaatatttac cagcagatac
    21421 taactcactt ctagctgtta tggataaagc gtttaaagct gaagataaaa ttaatttaat
    21481 tgtgacatct aagcaccctc gtccacaatt ttactctatt gctgaagcag aagagttagt
    21541 ggcagaagga tataaggtga ttgactgggc ttcaaatgtg tcgcttaatc aagagccaga
    21601 tgttgtcttt gctgcggcgg gaacagaacc taacttagaa gctttggcag ctattagcat
    21661 tcttcataag gctttcccag aacttaagat tagatttgtt aatgtattag acatcctaaa
    21721 acttcgtcat ccttcacaag atgctcgcgg tttatcagat gaagagttta acaaagtctt
    21781 taccacagat aaacctgtta tttttgcttt ccatggttat gaagatatga ttcgagatat
    21841 tttctttagt cgtcacaatc ataatttgca tacgcatggt taccgtgaaa atggtgacat
    21901 cacaacacca tttgatatgc gcgtgatgtc agaattggac cgattccatc tggcacagga
    21961 tgcagctctt gcttctttag gaaataaagc acaagccttc agtgatgaaa tgaatcaaat
    22021 ggttgcttat cacaaagact atattcgtga acacggagac gatattccag aagtacaaaa
    22081 ttggaaatgg gaaaatatca agtagtcact tttttcaggc ctatacttac aaacaaaaac
    22141 acttttagat tttttctaag ggtgttttgt tattttatag aagaatgacc gctttttcaa
    22201 aagtattaag tggtcacttt taaagttgta atattaatac taataagtct ttaaataatt
    22261 caataatgcc ataacaataa tggtgtgggc taattaaaat atctctaagc aaaggtgttg
    22321 acataaagag accatacatg atattatagt tatgttctgt ttcggcagtt tatttttttg
    22381 agtaaaaaag tgactaccct tttacgataa cttaacgtca ctttatagat aagacacata
    22441 accttaaaaa taagatacga aatttttagt aggagataag aatgacaaat attttagaag
    22501 tgaagaattt aaccaaaatt tttgggaaaa agcaaaaagc tgctcttgaa atggttaagc
    22561 aaggaaaaag taagacagaa attcttgaaa aaacaggagc gactgtaggt gtctatgatg
    22621 cgagttttga aataaaagaa ggcgaaattt tcgttatcat gggactttct ggttcaggaa
    22681 aatcaacttt agttcggatg ttaaaccgtt tgattgaccc atcatcagga aatatctatt
    22741 tagatggtaa agatattgca aagatgaatg tagaagattt acgtaacatt cgtcgtcatg
    22801 atatcaacat ggttttccaa aattttggtc ttttcccaca tcgtacaatt ttagaaaata
    22861 cagagtttgg gttagagatg cgtggtgttt ctaaggaaga acgtacaacg ttagctgaaa
    22921 aagctttgga caatgcaggt ctattaccgt ttaaagatca gtaccccagt caattatcag
    22981 gaggaatgca acagcgtgtt ggtttagcac gtgcccttgc taacagccct aaaatccttc
    23041 tgatggatga ggccttctca gctttagacc cattaattcg tcgtgaaatg caagatgagc
    23101 ttcttgatct tcaagacacg aataaacaaa ctattatttt tatcagccat gatttgaatg
    23161 aagcgttacg tattggtgat cgtattgctc tgatgaaaga tggagaaatc atgcagattg
    23221 gtacaggaga agagattttg actaatccgg ctaatgattt tgttcgtgag tttgttgaag
    23281 acgttgatcg ctcaaaagtg ttaactgctc aaaatattat gataaaacct ttaacaactg
    23341 ttttagaaat tgatggccca caagttgctt taacaagaat gcatcgtgag gaagtctcaa
    23401 tgttaatggc aacgaatcgt cgtcgccaac ttctgggatc cctaactgca gatgctgcaa
    23461 ttgaagctcg taagaaaggt ttgccgcttt ctgaagtgat tgataaagat gttgtaacgg
    23521 tctcaaaaga tacagttatt acagatatca tgcctttaat ttatgattca tctgctccga
    23581 tagccgttac tgatgataat gatagactcc taggggttat cattagagga cgtgtgatcg
    23641 aagcacttgc taacgttcaa gatgaaacag tagtagaatc gccaaaagaa acggtagaag
    23701 cttaatagag aggacaattc attttggaaa atttattaca acataagtta ccggtcgcac
    23761 catttgtaga gtcaacgacc aattggatta cgaaaacttt ttctggttta tttgacttta
    23821 tccagacaat cggtaatgca ttgatggatt ggatgacaaa aacattacta ttcattaatc
    23881 ctttactttt tattgtacta attacaattg ctgtattttt cttagctaag aaaaaatggc
    23941 aattaccgac atttactttc attggtttgc tatttatcta taaccaaggg ctgtgggaac
    24001 agttgattaa tacttttaac ttggttttag tagcaagttt aatttctatt attattggtg
    24061 taccactagg tatttggatg gcaaaaagtg ataaagttaa acaagttgtt aatccaattc
    24121 ttgactttat gcaaactatg ccggcattcg tttaccttat tcctgcagta gccttctttg
    24181 gtatcggtat ggtaccaggt gtttttgcct ctgttgtatt tgctctgcct ccaacagtgc
    24241 gctttactaa cttggctatt cgtgaaatcc cacttgaatt aatagaagca tctgattcat
    24301 ttggttcaac agctaagcaa aaattattca aagttgaact accacttgct aagaacacta
    24361 ttatggcagg aatcaatcaa actatgatgt tggctctttc tatggtggtt acaggatcta
    24421 tgatcggtgc acctggactt ggtcgtgagg tgttatcggc gcttcaacat gctgatattg
    24481 gtacaggatt tgttagtggt ctatcgctag ttattttagc aatcgtgtta gaccgtgtta
    24541 gtcaattctt taattccaaa ccgggtgaga agcaagctaa aacatcaaaa gttaaaaaat
    24601 gggtaggttt aggggccctg gctctcttca ttctagcagc tcttggtcgc attgttgtca
    24661 atatgacttc aggaaatgaa gccaaaggtc aaaaagtgaa gatagcttat gtccaatggg
    24721 attcagaagt tgcttcaacc aacgttattg ctgaagtttt aaaatctaaa ggatatgatg
    24781 ttgaattgac accacttgac aatgcggtca tgtggcaaac agtcgccaat gggaatgctg
    24841 attttacaac aagtgcttgg ttaccaaaga cccacgggca atattttaat aaatacaaaa
    24901 atagtttaga tgacttaggt cctcacgttg agaacgtcaa aataggttta gttgttccta
    24961 aatacatgaa cgtaaatagt attgaagaat tatcaaatca agcagataaa caaattacag
    25021 gtatcgaacc aggtgcaggt attatgaagt ctgctaaaca atcccttaaa gattatccaa
    25081 acctttcaag ttggaagttg ttatcagcat caacaggagc tatgacaaca accttaggga
    25141 aggctatcaa aaacaaagat caagtagtca ttacaggttg gtcacctcac tggatgtttg
    25201 ctaagtatga tttaaaatac cttaaagatc ctaagaaatc atttggtggt gaagaacata
    25261 tcaatacaat agcacgcaag aatttgaaaa aagatatgcc aaaagtatat aaaattatcg
    25321 ataagttcaa gtggactaaa gaagatatgg aatctatcat gttagatatg gataaaggta
    25381 tggagcctgc taaagcagct caaaaatgga ttaaaaacca caaaaaagaa gtttcagagt
    25441 ggactaaata gtatagaaaa aaacacgtct ctcttagagc gtgtttttta atgtgactta
    25501 agtcgtttta gctatttaat ataagtttta tattatttta atacatcaaa agtgagagaa
    25561 tgaaagaaat ggcaggttat aaagtattac taagctaaat tctcaaagta agttccacct
    25621 ctagtagaaa tggaatttag tctgataaaa accaataaaa accaacgaaa atccgtctca
    25681 gactaagttc cgttggtttt tataatactt gatttcatag gttttcagag ttaaaaatcg
    25741 caaaaaaagg gtacaagaaa acatcctcat ctgaaagcgt
//
LOCUS       DATROD010000018        22435 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.018, whole genome shotgun sequence.
ACCESSION   DATROD010000018 DATROD010000000
VERSION     DATROD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22435
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.018"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(76..810)
                     /locus_tag="VBC74_000736"
     CDS             complement(76..810)
                     /locus_tag="VBC74_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019805208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6941706.1"
                     /translation="MVEPIISIQGLHKSFGKNEVLKGIDLDIHQGEVVVIIGPSGSGK
                     STFLRTMNLLEVPTKGTVTFEGIDITDKKNDIFKMREKMGMVFQQFNLFPNMTVLENI
                     TLSPIKTKGLSKLDAQTKAYELLEKVGLKEKANAYPASLSGGQQQRIAIARGLAMNPD
                     VLLFDEPTSALDPEMVGEVLTVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMDAGII
                     VEQGTPKKVFEQTKEIRTRDFLSKVL"
     gene            complement(803..1495)
                     /locus_tag="VBC74_000737"
     CDS             complement(803..1495)
                     /locus_tag="VBC74_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6941707.1"
                     /translation="MNFSFLPQYWSYFNYGVMVTIMISTCVVFFGTIIGVLIALVKRT
                     NLHFLTILANFYVWVFRGTPMVVQIMIAFAWMHFNNLPTISFGVLDLDFTRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPSGQIEAAYSLGIRPKNTLRYVILPQAFKNILPALGNE
                     FITIIKDSALLQTIGVMELWNGAQSVVTATYSPVAPLLFAAFYYLMLTTILSALLKPM
                     EKYLGKGVKIDG"
     gene            complement(1636..1866)
                     /locus_tag="VBC74_000738"
     CDS             complement(1636..1866)
                     /locus_tag="VBC74_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HEO6941708.1"
                     /translation="MESHLVRIINRLELMATDGGNLKRNFEREGVVVAEVSFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            2164..4425
                     /locus_tag="VBC74_000739"
     CDS             2164..4425
                     /locus_tag="VBC74_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6941709.1"
                     /translation="MLCQNCKLNESTIHLYTNVNGKQKQVDLCQNCYQIIKTDPNNPL
                     FSGLNHVSHAPGGINPFFDDFFGDLNNFRAFNGQDLPNTPPTQAGGNRGGGNGNGRNN
                     NRNQTATPPQAKGILEEFGINVTEIARHGDIDPVIGRDSEIIRVIEILNRRTKNNPVL
                     IGEPGVGKTAVVEGLAQKIVDGNVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKL
                     MEEIRQRQDVILFIDEIHEIVGAGTAGEGSMDAGNILKPALARGELQLVGATTLNEYR
                     IIEKDAALERRMQPVKVDEPSVEETITILKGIQKKYEDYHHVKYNNDAIEAAAVLSNR
                     YIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDQRLIEAENLKAQATREEDYERAA
                     YFRDQIAKYKEMQQQKVDDQDTPIITEKTIEHIIEEKTNIPVGDLKEKEQSQLINLAD
                     DLKQHVIGQDDAVIKIAKAIRRNRVGLGSPNRPIGSFLFVGPTGVGKTELSKQLAIEL
                     FGSADSMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEKVRRNPYSLILLDEIE
                     KAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGSGKTEASVGFGASREGR
                     TNSVLGQLGNFFSPEFMNRFDGIIEFKALDKENLLNIVDIMLSDVNARLAINGIHLDV
                     TDKVKEKLVDLGYDPKMGARPLRRTIQEHIEDAITDYYLENPSEKELRAIMTSNGNII
                     IKSSKKTEESTKG"
     gene            4611..4997
                     /locus_tag="VBC74_000740"
     CDS             4611..4997
                     /locus_tag="VBC74_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013642821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6941710.1"
                     /translation="MTNPTFGEKIDNVDYRSRFGVYAIIPNPTHDKIILVQAPNGAWF
                     LPGGEIEENENHLEALTRELIEELGYSATIGHYYGQADEYFYSRHRDTYYYNPAYIYE
                     VTAYHKEQAPLEDFNHLAWFPIQEAK"
     gene            5130..5432
                     /locus_tag="VBC74_000741"
     CDS             5130..5432
                     /locus_tag="VBC74_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEO6941711.1"
                     /translation="MRLINTTSSHPELVRNQLQNTDAKLVEVYSAGNTDVVFTTAPKH
                     YELLISNKYRAIKDEELEAIREFFLKRKIDQSIIIQEQMKSLHTAKLIEISYPTTA"
     gene            complement(5502..8294)
                     /locus_tag="VBC74_000742"
     CDS             complement(5502..8294)
                     /locus_tag="VBC74_000742"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEO6941712.1"
                     /translation="MKLKETLNLGQTAFPMRAGLPNKEPQWQEAWDQADIYKKRQALN
                     EGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFRAPYVPGWDTHGLPIE
                     QVLAKKGVKRKEMDLAEYLEMCRDYALSQVDKQRDDFKRLGVSADWENPYITLTPDYE
                     ADQVRVFGAMADKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTVTASRGLTVGPDMEYVVVAPVGSERKYLLAEVLVDSLAAK
                     FGWENFEIVTHHTGKELNHIVTEHPWDTEVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLDVVVTVDSRGLMMENAGPDFEGQFYDKVTPLVKEKLGDLLLASEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKTNFQPEWGKKRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFAEYGSIVWWQRDAKDLLPAGY
                     THPGSPNGLFEKETDIMDVWFDSGSSWNGVMNARENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAVLSQGFVLDGKGEKMSKSLGNTILPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILKQTSETYRKIRNTLRFLIANTSDFNPKQDAVAYENLGAVDRYMT
                     IKFNQVVDTINKAYAAYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSPERRR
                     MQTVFYDILVKLTKLLTPILPHTAEEIWSYLEHEEEEFVQLAEMPVAQTFSGQEEILE
                     EWSAFMTLRTQAQKALEEARNAKVIGKSLEAHLTIYASQEVKTLLTALNSDIALLMIV
                     SQLTIADEADKPADSVSFEGVAFTVEHAEGEVCERSRRIDPTTRMRSYGVAVCDASAA
                     IIEQYYPEAVAQGFEA"
     gene            complement(8579..9349)
                     /locus_tag="VBC74_000743"
     CDS             complement(8579..9349)
                     /locus_tag="VBC74_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEO6941713.1"
                     /translation="MPLTALEIKDKTFSSKFRGYSEEEVNEFLEIVVDDYEDLIRRNR
                     EQEQYIKDLEEKIAYFNEMKESLSQSVILAQETAERVKISAQDEASNLMGKATFDAQH
                     LIDEAKLKANQILRDATDDAKRVAIETEDLKRQSRVFHQRLLSELEGQLKLANSSAWE
                     ELLKPTAIYLQNSDASFKEVVEKVLDEDDALPVVDDTESFDATRQFSPDEMEELQRRV
                     EESNKQLEESGLLDTNNFQMEEPINLGETQTFKLNIED"
     gene            complement(9359..10147)
                     /locus_tag="VBC74_000744"
     CDS             complement(9359..10147)
                     /locus_tag="VBC74_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEO6941714.1"
                     /translation="MTLDDIYQHFRPEEYAFINKIDHLAQYVENTYSFITTEFLNPRE
                     FKILESVLERRGSHYYTSGQYFQTEYVKVIIAPEYYQLDMVDFNLSLIEIKYNAKFNH
                     LTHAKIMGTLLNYLGVKRSILGDILVEEGCAQVLVDSQMTNHLVHSVTKIGTASVQLA
                     EVPLSKLLTPKQDIQKLTVIASSLRLDKILATILKISRTQSTKLIEADKVKVNYAIVN
                     RVSEQLVEGDLISVRGYGRFTLNHNLGLTKNQKYKLEVDKMIHN"
     gene            complement(10149..10403)
                     /locus_tag="VBC74_000745"
     CDS             complement(10149..10403)
                     /locus_tag="VBC74_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEO6941715.1"
                     /translation="MILVLLILLKTIRVYSYILLAYALLSWLPGAYDSWIGRLVVQIV
                     DPIIRPLRRFNLQFLGLDFTVIVAFFLLNMLSRFLILLFT"
     gene            complement(10406..11011)
                     /locus_tag="VBC74_000746"
     CDS             complement(10406..11011)
                     /locus_tag="VBC74_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEO6941716.1"
                     /translation="MALKDRFDKIISYFDTDDVSENEVHEVQERTSVQRDSRAATAQE
                     ASQRSHMTNSAEEEMIGSRPRTSTYNPNRQERQRVQRDNAYQQATPRIQNKDSVRQQR
                     EQVTIALKYPRKYEDAQEIVDLLIVNECVLIDFQYMLDAQARRCLDYIDGASRVLYGS
                     LQKVGSSMFLLTPANVMVDIEEMNIPKTGQETSFDFDMKRR"
     gene            complement(11023..11697)
                     /locus_tag="VBC74_000747"
     CDS             complement(11023..11697)
                     /locus_tag="VBC74_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEO6941717.1"
                     /translation="MMNLQENKTAIFDNVSKLALKAGRAPESVHIVAVTKYVNCQTTE
                     ALIRTGVNHIGENRVDKFLEKYQALKDEKLTWHLIGSLQRRKVKDVINYVDYFHALDS
                     VKLAAEIQKRAQKPVKCFLQVNISREDSKHGFTIEQIDDALNLISRYDKIELIGIMTM
                     APLKATKEEISSIFEETESLRKRLQARNIERMPFTELSMGMSRDYDIAIQNGSTFVRI
                     GTSFFK"
     gene            complement(11703..12983)
                     /gene="ftsZ"
                     /locus_tag="VBC74_000748"
     CDS             complement(11703..12983)
                     /gene="ftsZ"
                     /locus_tag="VBC74_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEO6941718.1"
                     /translation="MVFSFDTASVQGAVIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEVLTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVITRPFGFEGNKRSNFAIEGIQELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERITEAARKAIYSPLLETTIDGAEDVIVNVTGGMDMTLTEA
                     EEASEIVSQAAGKGVNIWLGTSIDMDMKDEIRVTVVATGVRKDKTNQVSGFTTSAPTN
                     QAPSERQSTSNSNFDRRGNFDMTESREMPTQQNQPHAQNQQQSSAFGNWDLRRDNISR
                     PTEGELDSKLSMSTFSENDDMDDELETPPFFKNR"
     gene            complement(13005..14378)
                     /gene="ftsA"
                     /locus_tag="VBC74_000749"
     CDS             complement(13005..14378)
                     /gene="ftsA"
                     /locus_tag="VBC74_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEO6941719.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFIANEMNVIGVSNVPSSGVKD
                     GIIIDIEAAATAIKEAVKQAEEKAGITIDKINVGLPANLLQIEPTQGMIPVPNESKEI
                     KDEDVESVVKSALTKSITPEREVISLIPLEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPTTILHNLRKTVERAGIKVEHVVIAPLALAKSVLNEGEREFGATVIDMGGGQTTVAS
                     MRNQELQYTNIYSEGSDYVTKDISKVLRTTVEIAEALKFNFGQANVEEASTSDTVQVN
                     VVGNEEPVEITESYLSQIISGRIRQILEHVKQDLGRGRLLDLPGGIILVGGGAIMPGV
                     VEVAQQIFGTRVKLHVPNQVGIRNPMFANVISIVDYVGMMSEVDIIAQHAVTGDEMLR
                     HKPVDFDYKEKTNTMSTMPYSEPLTSSMEDSNLEPIRARENAQEPTEPKTNIGERIRG
                     IFGSMFD"
     gene            complement(14651..15787)
                     /locus_tag="VBC74_000750"
     CDS             complement(14651..15787)
                     /locus_tag="VBC74_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEO6941720.1"
                     /translation="MPKKKSDTPEKEEVVLTEWQKRNLEFLKKRKEDEEEQKRINEKL
                     RLDKRSKLNISSPEEPQNTTKIKKLHFPKISKPKIEKKQKKEKIVNSLAKTNRIRTAP
                     IFVVAFLVILVSVFLLTPFSKQKTITVSGNQHTPDDILIEKTNIQKNDYFFSLIFKHK
                     AIEQRLAAEDVWVKTAQMTYQFPNKFHIQVQENKIIAYAHTKQGYQPVLETGKKADPV
                     NSSELPKHFLTINLDKEDSIKLLIKDLKALDPDLISEIQVISLADSKTTPDLLLLDMH
                     DGNSIRIPLSKFKERLPFYKQIKKNLKEPSIVDMEVGVYTTTSTIESTPVKAEDTKNK
                     STDKTQTQNGQVAENSQGQTNNSNTNQQGQQIATEQAPNPQNVN"
     gene            complement(15791..16867)
                     /locus_tag="VBC74_000751"
     CDS             complement(15791..16867)
                     /locus_tag="VBC74_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEO6941721.1"
                     /translation="MGKKIVFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     EQINQSGLDITFHSIATGKLRRYFSWQNMLDVFKVGVGVLQSIAIIAKLRPQALFSKG
                     GFVSVPPVVAARLLKVPVFVHESDLSMGLANKIAYKFATIMYTTFEQSKDLIKTKHIG
                     AVTKVMDCKKSFENTDLTSIKEAFDPNLKTLLFIGGSAGAKVFNDFITQTPELEEKYN
                     VINISGDSSLNRLKKNLYRVDYVTDLYQPLMNLADVVVTRGGSNTIFELVAMKKLHLI
                     IPLGREASRGDQLENAAYFEEKGYALQLPESELNINTLEKQINLLISNSESYEKNMSQ
                     SSEIKSQDEFYQLLIDDMAKVTKG"
     gene            complement(16870..18225)
                     /locus_tag="VBC74_000752"
     CDS             complement(16870..18225)
                     /locus_tag="VBC74_000752"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEO6941722.1"
                     /translation="MKTITTFENKKVLVLGLARSGEAAARLLAKLGAIVTVNDGKPFD
                     ENPTAQSLLEEGIKVVCGSHPLELLDEDFCYMIKNPGIPYNNPMVKKALEKQIPVLTE
                     VELAYLVSESQLIGITGSNGKTTTTTMIAEVLNAGGQRGLLAGNIGFPASEVVQAADD
                     KDILVMELSSFQLMGVKEFRPHIAVITNLMPTHLDYHGSFEDYVAAKWNIQNQMSSSD
                     FLVLNFNQGISKELAKTTKATIVPFSTTEKVDGAYVQDKQLFYKGENIMSVDDIGVPG
                     SHNVENALATIAVAKLAGISNQVIRETLSNFGGVKHRLQSLGKVHGISFYNDSKSTNI
                     LATQKALSGFDNTKVILIAGGLDRGNEFDELIPDITGLKHMVVLGESASRVKRAAQKA
                     GVTYSDALDVRDAVHKAYEVAQQGDVILLSPANASWDMYKNFEVRGDEFIDTFESLRG
                     E"
     gene            complement(18355..18600)
                     /locus_tag="VBC74_000753"
     CDS             complement(18355..18600)
                     /locus_tag="VBC74_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEO6941723.1"
                     /translation="MFYLIVALLIALYYFFMAPKTVRNTLNAIGLVGLVALLLVLAVM
                     SFIKILQLPGELYIGLIMIPLGYTAFKETLNLSEKKK"
     gene            complement(18645..20486)
                     /gene="typA"
                     /locus_tag="VBC74_000754"
     CDS             complement(18645..20486)
                     /gene="typA"
                     /locus_tag="VBC74_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEO6941724.1"
                     /translation="MTNLRTDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELEER
                     AMDSNDIEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPSEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSMSDDPSDQEKTMAPIFDTIIDHIPAPVDNSEEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERKEIQEAKAG
                     DLIAVSGMEDIFVGETVTPTDAIEPLPVLRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVQCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGVLVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLADDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            complement(20718..21098)
                     /locus_tag="VBC74_000755"
     CDS             complement(20718..21098)
                     /locus_tag="VBC74_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO6941725.1"
                     /translation="MDMSVILIIVILLAFVAWASWNYWRVRRAAKFLDNESFQKEMSR
                     GQLIDIREAGAFHRKHILGARNIPASQFKVALSALRKDKPVLLYDASRGQSIPRIVLL
                     LRKEGFNQLYVLKDGFNYWTGRVK"
     gene            complement(21110..22078)
                     /locus_tag="VBC74_000756"
     CDS             complement(21110..22078)
                     /locus_tag="VBC74_000756"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEO6941726.1"
                     /translation="MSKKLLGIDLGGTTIKFGILTLEGEVQEKWAIETNTLENGRHIV
                     SDIVESLKHRLSLYGLTKDDFLGIGMGSPGAVDRTSKTVTGAFNLNWADTQEVGSVIE
                     KEVGIPFFIDNDANVAALGERWVGAGANNPDVVFVTLGTGVGGGVIADGNLIHGVAGA
                     GGEIGHMIVDPENGFTCTCGNKGCLETVASATGVVRVARQLAEQYEGSSAIKAAIDNG
                     DTVTSKDIFIAAEDGDKFANSVVERVSRYLGLAAANISNILNPDSVVIGGGVSAAGEF
                     LRSRVEKYFVTFAFPQVKKSTKIKIAELGNDAGIIGAASLANQQAS"
     gene            complement(22075..22284)
                     /locus_tag="VBC74_000757"
     CDS             complement(22075..22284)
                     /locus_tag="VBC74_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEO6941727.1"
                     /translation="MKVLFDVQNLLKKFGIYVYIGKRLYDIEVMKIELQRLYDNGLIS
                     RDDYLKAELILRREHRLELEKENKK"
BASE COUNT         7055 a         4453 c         3599 g         7328 t
ORIGIN      
        1 caaacaatac agtaacaatt tactcataac aatggaataa taaaagtcta aaacaagttg
       61 ttttagacaa tgaaattata atactttact taagaagtct cttgtgcgga tttcttttgt
      121 ctgctcaaat actttcttag gggtcccttg ctcaacaata atccctgcat ccataaaaat
      181 gacacgatcc gctacttcac gtgcaaaacc catttcatga gtgacaataa ccatcgtcat
      241 accagattta gctaaatctt gcataacagt caagacttca cctaccattt caggatcaag
      301 agctgaagta ggttcatcaa aaagaaggac atcaggattc attgcaagac ctcttgcaat
      361 agcaatccgt tgttgttgtc ctccagataa gcttgctgga taagcattag ccttctcttt
      421 gagtccaact ttttcaagta gctcgtatgc ttttgtctga gcatcaagct tagaaagtcc
      481 ctttgtctta ataggtgata aagtaatatt ttctagtaca gtcatattgg gaaatagatt
      541 gaactgttga aaaaccatgc ccattttttc gcgcatttta aaaatatcat tctttttgtc
      601 tgttatatca atcccttcaa aagtcactgt tccctttgtt ggtacttcca agagattcat
      661 tgttcttaaa aatgttgact taccagagcc agaagggcca ataataacca ccacttctcc
      721 ttgatgaata tccaagtcaa tgccttttaa aacctcattt ttcccaaaac ttttatgaag
      781 tccttgtatt gaaataattg gctcaaccat ctatttttac ccctttccca agatatttct
      841 ccattggttt taacaaagct gagagaatcg ttgtcaacat taaatagtaa aatgctgcaa
      901 ataataacgg tgcaactggt gagtaagtag ccgttacaac tgattgtgct ccgttccata
      961 attccatgac accaatagtt tgaaggagag cactatcttt aataattgta ataaattcat
     1021 tccctagagc aggtaaaata tttttaaaag cttggggtaa gataacatag cgaagtgtat
     1081 ttttaggtcg aatccccaac gagtaagctg cttctatttg tccagatggt acagcctcaa
     1141 tccctgcacg tacaatttcc gaaatatagg caccactatt taaggaaatg ataatgatac
     1201 caggtaaaag tcgtgtaaaa tctaaatcta aaacaccaaa gctaattgtt ggtaaattgt
     1261 taaaatgcat ccatgcgaaa gcaatcataa tttgaactac catcggtgtc ccacgaaata
     1321 cccatacata gaaattagct aatattgtga gaaaatgtaa attagtacgc tttactaaag
     1381 caattaacac gcctataata gttccaaaaa aaacaacaca tgttgaaatc ataatggtta
     1441 ccatcacacc ataattaaaa taggaccaat attgtggtaa aaatgaaaaa ttcattgaca
     1501 tctcctaaaa aagtaattgc caatagtcta tcacacaaag aataaaaata caagtttaaa
     1561 taaatatttt tgtttttatt ctattttaat gtatatttag atataaaaaa agaccttcaa
     1621 ggtcttttat aattatcagt ataataaatc gtaaatttcc attgcgataa ggtcaatgct
     1681 gtcaaatgaa tatgattcac gcgcttctac atcacgtaat gtgaaaacag gaccgttttc
     1741 agggtcatta ctaaaagata cttctgctac aacaacacct tcacgttcaa aattacgttt
     1801 taaattacca ccatcagtcg ccattaattc gaggcgattg atgattctca ctaaatgtga
     1861 ttccatctta cttactccta tatattttct agtcttttct attttaacac aaaagtgacc
     1921 aaagcaacat gaaaagcaac atttttacta actttttaac gattttatcg tctaaaatcc
     1981 gcaaataacg atttttctca gtagtcatct atttttcaat ttagcactct aatatattca
     2041 gtgctaaaaa cagaactttt ttcttgattt ctatgcttta ggtgctataa taaaagcata
     2101 aagtttgact atatttgact atatacacat caatagttaa atgtcataag aaaggggtag
     2161 cctatgctct gtcaaaactg caaactaaac gaatcaacta ttcatttgta tacaaatgtt
     2221 aatggaaaac aaaagcaagt tgatctttgt caaaattgct accaaattat taaaactgac
     2281 cctaataatc cacttttttc aggtttaaat catgtttcac atgcgcctgg tggcatcaat
     2341 cctttctttg atgatttctt tggagattta aataatttca gagcctttaa tggccaagat
     2401 ttaccaaaca ctcctcctac acaagcagga ggaaaccgag gtggaggaaa cggtaatgga
     2461 cgaaataata atcgcaacca aactgcaact cctcctcaag ccaaagggat tctagaagaa
     2521 tttggtatca atgtcactga aatagcccgc catggtgata ttgaccctgt tattggacgt
     2581 gactcagaaa ttattcgtgt tatcgaaatc cttaatcgtc gtacaaaaaa taatcctgta
     2641 cttattggcg aacccggcgt agggaaaact gccgttgttg aaggacttgc tcagaaaatt
     2701 gttgatggta atgtgcctca taaacttcaa ggcaaacaag ttattcgttt ggatgttgtg
     2761 agtctagtac aaggaacggg cattcgtggt caatttgaag aacgcatgca aaagttgatg
     2821 gaagaaattc gtcaacgtca agatgttatt ctattcatag atgaaattca cgaaatagtt
     2881 ggagctggta cagctggcga aggtagtatg gatgcaggta atattttaaa acctgccctt
     2941 gcacgtggag aattacaact agtaggtgcc actactctca atgaatatcg tattatcgaa
     3001 aaggacgctg ccttagaacg ccgtatgcaa cctgtaaaag tcgatgaacc ttctgttgaa
     3061 gaaaccatta ctattttgaa aggtatccaa aaaaaatacg aagattatca tcacgtaaaa
     3121 tataataatg atgccataga agcagctgca gtactatcta atcgttatat ccaagaccgc
     3181 tttttacctg ataaagcaat agacttatta gatgaagctg gttctaaaat gaacctaaca
     3241 ctaaattttg ttgatccaaa agaaattgat caacgtctca ttgaagcaga aaatttaaaa
     3301 gcgcaagcga ctcgtgaaga agattacgaa cgtgcagctt acttccgtga ccagattgca
     3361 aaatataaag aaatgcagca acaaaaggtc gacgatcaag atacacctat tattaccgaa
     3421 aaaacaattg agcacatcat tgaagaaaaa acgaatatcc ctgttggtga tttaaaagaa
     3481 aaagaacaat ctcaattaat taatctcgca gatgacttga aacagcatgt gatcggccag
     3541 gatgacgctg tcattaagat tgcaaaagct attcgtcgta atcgagttgg tcttggtagc
     3601 ccaaaccgtc ctattggttc ctttttattt gtaggaccaa ccggtgttgg taaaactgaa
     3661 ctttctaaac aactagcaat tgagctcttt ggttcagctg atagtatgat tcgttttgat
     3721 atgtcagagt acatggaaaa gcatgctgtt gctaaattag tcggagcgcc tccaggatac
     3781 gtgggatacg aggaagctgg acaactaact gaaaaggttc gtcgaaatcc ttactcgctc
     3841 atccttctag atgaaattga aaaagctcat cccgatgtca tgcatatgtt cttgcaggtc
     3901 cttgatgacg gtcgattaac agatggacaa ggaagaactg ttagttttaa agataccatt
     3961 atcatcatga cctcaaatgc tggttctggt aaaactgaag caagtgttgg ctttggtgcc
     4021 tcacgagaag gtaggacgaa ttccgtacta ggtcaactag gtaacttctt cagccctgaa
     4081 tttatgaacc gctttgacgg tattattgaa ttcaaggctt tagataaaga gaatctcctc
     4141 aatatcgttg atattatgtt atctgacgtt aatgcacgtc tcgccatcaa tggtattcat
     4201 ctagatgtca ctgataaagt gaaagaaaaa ttggttgatt taggttacga tcccaaaatg
     4261 ggagcacgtc cattacgtcg taccattcaa gaacatattg aagatgctat cacagattac
     4321 tatcttgaga atccaagcga aaaagaactt cgtgctatta tgactagcaa tggaaatatc
     4381 ataataaaat cttctaaaaa aactgaggaa agtacaaaag gttgagtaag aaactcaacc
     4441 ttttttcaca ctcttctcaa aaaataatag cctctataaa gctgtttctc tcctatctcg
     4501 tctttaaatt ttaatacaat tattaaggaa atattccaaa aattttagta taataaataa
     4561 aaaataatta aagcttttaa aatcataaaa cacaatcagg agagagaact atgactaatc
     4621 ctactttcgg tgaaaaaata gataatgtag actataggtc acgatttggt gtctatgcta
     4681 ttattccaaa tccaactcat gataaaatta ttttagtaca ggcacctaat ggtgcttggt
     4741 ttcttccagg tggggaaatt gaagaaaatg aaaatcacct agaagcttta actcgtgagt
     4801 taattgaaga attaggttat tctgcaacaa ttggccatta ctatggtcaa gctgatgaat
     4861 atttttactc tagacaccgt gatacttact actataatcc cgcctatatt tatgaagtta
     4921 ctgcctatca taaggagcaa gcgccactag aagatttcaa ccatctagcc tggtttccta
     4981 tccaagaagc taaataaaag ctaaaacgag gaagccatag atggggtgtc caagcttggg
     5041 aaaaaaatca ccattctagg aaataattca tacctttctt cctaaatagt gttataataa
     5101 acctaataag aatcaggggg aaaatagtta tgagactaat taatacaacc agtagtcatc
     5161 ctgaactagt tcgaaatcaa ctccaaaata cagatgcaaa attagtggaa gtctattcag
     5221 ctggcaacac cgatgtcgtt tttacaacag cacctaaaca ttatgaatta ttaatttcaa
     5281 acaaatatcg tgctatcaaa gatgaagaat tagaagctat tcgtgagttc ttcttaaagc
     5341 gtaaaataga tcaatctatt attattcaag agcagatgaa atcactccat actgccaagt
     5401 taattgaaat ttcatatcca acaacagcat aattaaaaga agcctaatca aggcttcttt
     5461 tttgatgact actaaacaaa tatagcatta aataattttt attaagcttc aaaaccttga
     5521 gctactgctt caggataata ttgctcaata attgctgcac tagcatcaca aacagctaca
     5581 ccatacgaac gcatccttgt ggttggatca atacggcgtg agcgttcgca gacttcacct
     5641 tcagcgtgtt caacagtaaa tgcaacacct tcaaatgata ctgagtcagc tggtttgtct
     5701 gcttcatcag caattgttaa ttgtgagaca atcatcaaga gtgcaatatc gctatttaac
     5761 gctgttaata acgttttcac ttcttgacta gcataaattg ttaaatgtgc ttctagagat
     5821 ttaccaatga ctttagcgtt acgtgcttct tcgagagctt tttgagcttg tgttcgtaac
     5881 gtcatgaatg ctgaccactc ttcaagaatt tcttcttgac cagagaatgt ctgtgcaaca
     5941 ggcatttctg ctaattgaac aaattcctcc tcctcatgtt caaggtatga ccaaatttct
     6001 tcagcagtat gtggtagaat cggagtcaaa agttttgtaa gtttaactaa aatatcgtag
     6061 aaaacagttt gcatacgacg tcgttctgga ctgttagccg cttcaatgta aaccacatct
     6121 tttgcaaaat caaggtaaaa tgctgataga tcaactgtta cgaagttaac gacagcttta
     6181 taaattgcca taaaatcata agcagcataa gctttgttga ttgtatccac aacttgatta
     6241 aatttaatag tcatataacg atcaacggca cctaaatttt cataggctac tgcatcttgt
     6301 ttaggattaa aatcggaagt atttgcaatc aagaaacgca atgtatttct gatcttacga
     6361 taagtttctg atgtttgttt taagatatcc attgaaatac gaacatcatt acttgaatca
     6421 acagaagtta cccacagacg caagatttcc gcaccaaatt gtttttcaac atcactcgga
     6481 agaattgtat ttcctagaga tttagacatt ttctcacctt taccatcaag tacaaaacct
     6541 tgagataaga ctgctttgta tggagcgtga ccattaacag caactgatgt aattagtgat
     6601 gagttgaacc aaccacggta ttgatcagaa ccttcaagat aaagatctgc tggataggaa
     6661 aggttttcac gagcattcat tactccattc catgatgaac cagaatcaaa ccaaacatcc
     6721 atgatgtctg tttctttctc aaagagacca ttaggtgaac ctggatgagt ataacctgct
     6781 ggtagaaggt ccttagcatc acgttgccac caaacgattg agccgtattc agcaaataaa
     6841 tctgcaacgt gatcagtcac ttcttttgtc atgatagctg taccatcttc tgcataaaaa
     6901 attggaagtg gaacacccca agcacgttga cgtgagatta cccagtcgcc acggtcacgg
     6961 atcatgttat aaagacgttt tttaccccac tcaggttgga aatttgtctt ttcaatttca
     7021 tcgaggattt cttgacggaa tttcgaaaca gaggcaaacc attgtggaac cgcacgccaa
     7081 atgattggct ttttcgtacg ccagtcaaat ggatatgagt ggttgataac ttcgctggct
     7141 aaaagaagat ctcccaattt ttcttttaca agtggtgtca ctttgtcata gaattgacct
     7201 tcaaaatcag gaccagcatt ttccatcatc aaaccacgac tatctacggt aactacaaca
     7261 tcaagtccat tagcaatacc aacgttatag tcatcttcac caaaaccagg agccgtgtgg
     7321 acaatacctg taccagaatc tgttgtaaca tggtctccaa ggataaccaa ctcttctact
     7381 tctgtatccc atggatgttc tgtaacaatg tgattaagtt ctttaccagt gtgatgagtc
     7441 acaatttcaa agttttccca gccaaactta gcagcgagac tatctacaag aacctctgca
     7501 agaaggtatt tacgctcact acctactggt gcaactacaa catactccat atctggtcct
     7561 actgttaaac cgcgtgaagc tgttacagta aatggtgttg tcgtccaaac gacgatatag
     7621 gtatctgtat caagaattcc cttaccatct ttaactttat tggcatagta gagtgatgtc
     7681 gaatcaatat catgatattc gatttcagcc tcagcaaggg cagactctga tgaccatgac
     7741 caatacactg gtttagcacc acgatagata tatcctttat ctgccatagc accgaaaaca
     7801 cgtacttgat ctgcttcata atctggtgtc agtgtaatat aaggattttc ccaatcagca
     7861 gaaacgccca gacgtttaaa atcatcacgt tgtttgtcaa cttggctgag agcataatca
     7921 cgacacattt caaggtattc agccaagtcc atttctttac gtttaacccc ttttttagct
     7981 aatacttgct caattgggag tccatgagta tcccaaccag gcacataagg agctcgaaaa
     8041 cctgacattg attttgaacg tacaataatg tctttagaaa ttttatttag tgcatgtcct
     8101 acatgaatat tcccattagc atagggaggt ccgtcatgaa ggtgaaaggc tggttttcct
     8161 tcattcaatg cttgacgttt tttataaata tcagcttgat cccatgcttc ttgccattga
     8221 ggttccttat ttggaagccc tgcacgcatt ggaaaagctg tttgtcctaa attcaaggtt
     8281 tcttttaatt tcatactaac tccttatctc atagtaatag ttatcacaat tttttaaatt
     8341 gctaaataaa aaagctttca tcacataaag gacgaaagct cgtggtacca ccttaattca
     8401 gagcagaaaa tagctatttc ttactccctc aacaacccgt aaggtgggtt aagccttcta
     8461 ccttacttaa ataatagttc aggtagaatg attgaaaatg ataatttcaa tattagggat
     8521 tgtagggctc tcaccatctc ctactcgctg ataatataat attaaaacct tgtttttctt
     8581 aatcttcaat atttaattta aaagtttgag tttcacctaa attgatcggt tcttccattt
     8641 ggaaattatt agtatctaat aaacctgact cttcaagttg tttgttactt tcttcaacac
     8701 gacgctgtaa ttcttccatt tcatctggtg agaattgacg agtagcatca aatgactcag
     8761 tgtcatccac cactggtaaa gcatcatctt catcaagaac tttttcaaca acttctttga
     8821 aagaagcatc agaattttga agataaatag ctgttggttt caacaattct tcccaagcag
     8881 atgagttagc taattttaac tgaccttcta attcagaaag taagcgttgg tggaaaacac
     8941 gtgattgacg tttcaaatct tctgtttcta tagcgacacg cttagcatca tctgtcgcat
     9001 cacgaaggat ttgatttgct tttaatttag cctcatcaat taaatgttga gcatcaaatg
     9061 tagctttccc cattaggtta gatgcttcat cctgtgctga aattttcaca cgttcagcag
     9121 tttcttgagc taaaataact gattgactta acgattcctt catttcgttg aagtaagcga
     9181 ttttctcttc taaatcttta atgtattgct cttgctcacg attacgtcta atcaagtctt
     9241 cgtaatcgtc aacaacaatc tctaaaaatt cattaacttc ttcttcgcta taaccgcgaa
     9301 attttgatga aaatgtttta tctttaattt caagtgctgt aagtggcatg ttttctcctt
     9361 agttatgtat cattttgtct acttctaact tatatttttg atttttagtt aaccctaggt
     9421 tatggttcaa cgtaaatcga ccatatcctc taacactaat caaatccccc tctactaatt
     9481 gttcagaaac tcgattgacg attgcataat tgaccttgac tttatctgcc tcaatcaatt
     9541 tcgtcgactg tgttcgtgaa atctttaaaa tcgttgctaa aattttatca agacgtaaac
     9601 tggaagcaat aacagttagt ttttgaatat cttgtttggg agttagaagt ttggacaagg
     9661 gaacttcagc taattgtaca ctagcagttc caatttttgt aactgaatga acaaggtgat
     9721 ttgtcatttg actatcgacc aaaacctgag cacagccttc ttcaactagg atatccccta
     9781 aaattgatcg ttttacacct aagtaattca gtaaagttcc cataatttta gcatgggtaa
     9841 ggtgattaaa tttagcatta tactttattt caattaggct aaggttaaaa tcaaccatat
     9901 ctaactgata gtattccggt gctattatta ctttaacata ttctgtttga aaatactgac
     9961 cagaagtgta ataatgacta cctcgcctct ctagaacact ttcgagtatt ttaaattccc
    10021 taggatttag aaattcagtg gtaataaacg aataggtgtt ttcgacatat tgagctaaat
    10081 ggtctatttt attgataaac gcatactctt ctggtcgaaa atgctgataa atatcatcta
    10141 acgtcatctt atgtaaaaag caagattaaa aatctactca acatatttag taaaaagaat
    10201 gctactatta ctgtaaaatc tagacctaaa aattgtagat taaatcttct gagtgggcga
    10261 ataatcggat caacaatctg taccactagt ctaccaatcc atgaatcgta tgctcccgga
    10321 agccaagata acaaggcata ggctagtaag atataagaat agaccctaat tgtttttaat
    10381 aatattaata gtactaatat catatttatc gtctcttcat atcaaaatca aaactcgtct
    10441 cttgaccagt ctttggaata ttcatctcct caatatcgac cataacattg gctggtgtta
    10501 atagaaacat tgaactccca accttttgta atgaaccata taatactcta cttgcaccat
    10561 caatataatc caagcaacgt cgtgcttgcg catcaagcat atattgaaaa tcaatcaaga
    10621 cacattcatt aacgataagt aaatcaacaa tttcttgtgc atcctcatac ttacggggat
    10681 acttcagagc aattgttact tgttctctct gttgtcgaac tgagtcttta ttttgaatcc
    10741 taggagtagc ttgctgataa gcattgtctc tctgtaccct ttgtctctcc tgacgattag
    10801 gattataggt agaagttctc ggacgactac caatcatctc ttcctctgcg gagttagtca
    10861 tatgactacg ctgagaagct tcctgtgctg tagctgctct ggaatctctt tgcactgaag
    10921 ttctctcttg tacttcgtgt acttcatttt cacttacatc gtcagtgtca aaataagaaa
    10981 taattttgtc aaatctatct tttaatgcca tattcccctc cattatttaa agaatgaagt
    11041 tcctatcctt acaaatgttg atccattttg aatagcaata tcataatctc tactcatgcc
    11101 catgcttaat tctgtaaacg gcattcgttc tatgttccta gcttgaagcc ttttcctaag
    11161 actctcagtc tcttcaaaaa ttgatgatat ttcctcttta gttgctttta agggagccat
    11221 agtcatgatg ccgataagtt caattttatc gtaccgagaa attaagttca gtgcatcatc
    11281 tatttgctca atagtaaaac catgcttact gtcttctcgt gatatattaa cttgtagaaa
    11341 acatttaact ggtttttgag cacgtttttg aatctcagct gctaacttaa cagaatctaa
    11401 agcatgaaaa taatcaacat aattaatgac atctttaact tttcgacgtt gtaaactacc
    11461 aatgagatgc catgtaagct tttcatcttt taatgcttga tatttttcaa gaaatttatc
    11521 aacacgattt tcaccgatat gattaacacc tgttctaata agcgcttctg ttgtttgaca
    11581 gttaacatat tttgttacag ctacgatatg aactgattca ggagcgcgac ctgcttttag
    11641 tgctaattta ctaacattgt caaaaatcgc tgttttattt tcttgtaaat tcatcatttt
    11701 aattaacggt ttttaaagaa tggaggtgtt tcaagttcat catccatatc atcattttct
    11761 gaaaacgttg acatagacaa cttgctatca agttcacctt ctgtcggacg tgaaatatta
    11821 tcacgacgaa ggtcccaatt accaaaagca gaactttgtt gctgattttg tgcatgaggc
    11881 tgattttgtt gagttggcat ttcgcgagac tctgtcatat caaagttgcc acgacgatca
    11941 aagtttgaat ttgaagtact ttgacgttct gaaggtgctt gattagttgg agctgatgtt
    12001 gtaaaaccag atacttgatt agttttatct ttacgcacac ctgttgcaac aactgttacg
    12061 cggatttcgt ctttcatatc catgtcaatt gaagtgccta accaaatatt gacaccttta
    12121 cctgctgctt ggctaacaat ctctgatgct tcctctgctt cagtaagtgt catatccata
    12181 ccgccggtaa cattgacaat aacgtcttct gctccatcaa tcgttgtttc aagaagtggt
    12241 gaataaattg cttttcgagc agcttcagta atacgttcct caccagaacc gataccaatt
    12301 cccatcagtg cattaccttt atttgccata actgttttaa catctgcaaa gtcaaggtta
    12361 attaaacctg ggttagtaat caaatctgta ataccttgaa caccttgacg taaaacatta
    12421 tctgcttcac taagagcttc aagcaatggt gtcttcttat ctacaatttc aagaagatta
    12481 ttatttgaaa tgataagcag tgtatcaact tgttctctta attcttgaat accttcgata
    12541 gcgaagttag aacgtttatt accctcaaag ccaaaaggac gtgtgataac cgctactgta
    12601 agtgctccta aactctttgc aatacgtgca ataactggag ccgcacctgt accagatccg
    12661 ccccccatac ctgctgtgat aaataccata tcagcgccag taagtgcttc tgttaaaact
    12721 tcttcacttt cttcagctgc tttacgacca acttcaggtt gtcctcctgc accaagtccg
    12781 cgagttaatt ttggaccaag ttgaatgaca gtttcagctt ttgagctact taaggcttga
    12841 atatcagtat ttgctgcaat aaactcaacg ccagcgacac cttcgtcaat catgcggtta
    12901 atagcgttac cgccaccgcc tccaacacca ataactttaa taactgcacc ttgtactgat
    12961 gctgtatcaa atgaaaatac catttttatt cctcacttat tgttttagtc aaacatactt
    13021 ccaaaaattc ctctgatgcg ctcaccaata ttagttttag gctcagtagg ctcttgagcg
    13081 ttttctcttg ctcggattgg ttctaaatta gaatcttcca tagaagaggt taacggctca
    13141 gaataaggca ttgtcgacat tgtatttgtt ttttctttat aatcaaagtc gactggttta
    13201 tgtcttagca tttcgtcgcc agttacagca tgttgtgcga taatgtcaac ttctgacatc
    13261 attcctacat aatcaacgat actaataaca ttagcgaaca ttggattgcg aatacctact
    13321 tggtttggaa cgtgcaattt aactctagtt ccaaaaattt gttgagcaac ctcgacaact
    13381 cctggcataa ttgcaccacc tccaacaaga ataataccac caggtaaatc aagtaaacga
    13441 cctctaccca agtcttgttt gacatgttct aaaatttgac gaatacgtcc tgaaataatt
    13501 tgcgaaagat agctttctgt aatttcaaca ggttcctcgt taccaacaac attaacttga
    13561 actgtatcag aagtacttgc ttcttcaaca ttagcttgtc caaagttgaa tttcaatgct
    13621 tcggcaattt ctactgttgt acgtaacact ttagaaatat ctttggtgac ataatcgcta
    13681 ccttcagaat aaatattagt atattgcaat tcttgattac gcatagaagc gactgttgtt
    13741 tgtccgcctc ccatatcaat cacagttgca ccaaactcac gttcaccttc gtttagaact
    13801 gattttgcta aggctagagg ggcaatcaca acatgctcta cttttatacc tgctcgttca
    13861 acagttttac gaagattatg aaggatagtt gttggtccag tatagataag accacgcatc
    13921 tcaaggcgaa tccccatcat ccctctaggg tctctaatcc cttggaaacc atctacaatg
    13981 aattcaagcg gaatcaatga aataacttct ctttcaggag taatgctttt agtcaatgct
    14041 gatttaacaa cactttcgac atcttcatcc ttaatttctt ttgattcatt gggaacagga
    14101 atcattcctt gagttggttc aatttggaga agatttgctg gtaatccaac attgattttg
    14161 tcaatggtga tgcctgcttt ctcctcagct tgttttaccg cctctttgat tgcagttgct
    14221 gctgcctcaa tatcaataat tataccatct tttacaccgg aactagggac gttgctaacc
    14281 ccaattacat tcatttcatt tgcaataaat tctgcaacta aaactttaat cgagcttgtt
    14341 cctatatcca aaccagtaaa aaagccattt ctagccattc actcgacctc gctatctttc
    14401 cattcttttc tattctacat ctgccaaaaa cagaattatt cagaaaatat tataccataa
    14461 aaaaagcaga attgttacca aattacgttt ttttattaag caacatcaaa ttgaaatatt
    14521 tctaacttat ttaaaccatt cgcaacttat aacaaactta aatttttttc aagaaatcaa
    14581 tttttactag caatctatga ctgacttgat atgtataaaa aagactgata aatccagtct
    14641 ttttttatcg ctaattaaca ttttgagggt tgggtgcctg ctctgttgct atctgttgtc
    14701 cttgttgatt agtatttgag ttatttgttt gtccttgact attttccgca acctgaccat
    14761 tttgtgtttg tgttttatca gttgatttat tttttgtatc ttccgctttc acaggggttg
    14821 attcaatagt acttgttgtt gtgtaaactc ccacttccat atcaacaata gaaggttcct
    14881 taaggttctt cttaatttgt ttgtaaaaag gaagtctttc tttaaattta gataatggta
    14941 ttctaatact atttccatca tgcatatcta acagcaggag gtcaggtgtc gttttagaat
    15001 cagctaaact tatcacctga atctcactta ttaaatcagg gtctaaagcc tttaaatctt
    15061 taattaatag cttaatacta tcttccttat caaggttaat tgttaagaag tgctttggta
    15121 gctctgaact atttacagga tcagcctttt ttccagtttc caagacaggc tgatatcctt
    15181 gctttgtatg tgcatatgca ataatcttat tttcttgaac ttgaatatga aacttattgg
    15241 gaaattgata agtcatctga gctgttttta cccatacatc ttctgcagct aaacgttgtt
    15301 caatagcttt atgtttaaaa attaaagaaa agaaataatc gtttttttga atattcgttt
    15361 tttctatcaa aatatcatca ggtgtatgct gattgccact aactgttatt gttttttgct
    15421 tactaaaagg agttagtagg aaaacggaaa ctaaaatgac taggaatgct actacaaata
    15481 taggtgcagt tctaatgcga ttagttttgg ctaagctgtt gactattttt tcttttttct
    15541 gtttcttttc aatcttaggt tttgaaatct ttggaaaatg aagcttctta attttagtag
    15601 tattttgagg ttcttcagga gaagaaatat ttaatttact tcttttatct aagcgtaatt
    15661 tttcgttaat acgtttttgt tcttcttcat cttctttgcg tttttttaaa aattcaaggt
    15721 tacgcttttg ccattccgtt aagacaactt cttctttttc tggggtatct gatttcttct
    15781 taggcatgtt ttatcctttt gttacttttg ccatatcatc aattaataat tgataaaatt
    15841 catcttgaga ttttatttca gatgattgtg acatgttttt ttcgtaactc tcactgttag
    15901 aaattaataa atttatctgt ttctcaagtg tatttatgtt taattcagat tctggtaact
    15961 gcaatgcgta gcccttttct tcaaaataag cagcattctc taattggtca cctcggctcg
    16021 cttcacgtcc aagagggata ataagatgaa gttttttcat agctactaat tcaaaaatag
    16081 tattagagcc acctcgtgta acaaccacat ctgctaaatt cattaaaggc tgataaaggt
    16141 ctgtaacata atccacgcga taaaggttct tcttcaatcg attgagagaa gaatcgcctg
    16201 aaatattgat aacattatat ttttcttcca gctcaggtgt ttgagtaata aaatcattga
    16261 atacttttgc acctgctgat cctccaataa atagtaaggt ctttaggttt ggatcgaagg
    16321 cttcttttat actagttaag tcagtatttt caaacgactt tttacaatcc atgacttttg
    16381 ttacagcacc aatatgctta gttttgatta aatctttaga ttgttcaaaa gtcgtataca
    16441 taatagtcgc aaatttataa gcaatcttat ttgctaaccc cattgataaa tctgattcat
    16501 gaacaaaaac aggaactttt aataggcgtg cagctactac aggaggtact gaaacaaatc
    16561 ccccttttga aaataaggct tggggtctaa gtttagcaat aatagcaatc gattgaagaa
    16621 cgccaacccc tactttaaag acatctaaca tattttgcca tgaaaaatag cgacgtagtt
    16681 tcccagtagc tatagagtga aatgtgatat caagtccaga ctgattaatt tgttcatgtt
    16741 ctatgccatt tttatcacca atataatgta cttcccaacc atctttgata aatttgggga
    16801 ttaatattag gtttagtgtt acatgaccta ccgtaccacc acctgtaaaa acaatttttt
    16861 tccccatagt tactctcctc taagactttc gaaagtatca atgaattcat caccacggac
    16921 ttcgaaattc ttatacatgt cccatgatgc atttgcagga cttagcaaga taacatcgcc
    16981 ctgttgtgcc acctcataag ctttatgtac cgcatctcta acatctaaag catcgctata
    17041 agttactcct gctttttgtg cagcacgttt tactcgagat gccgattccc ctaaaacaac
    17101 catatgttta agtccagtga tatctggtat caattcatca aactcattac cgcgatcaag
    17161 acctcctgca attaggataa ctttagtatt atcaaagcca gataatgctt tttgagttgc
    17221 caatatatta gttgacttgc tgtcgttata gaaactaata ccatgaacct taccgagtga
    17281 ttgcaagcgg tgtttaacac ctccaaaatt gcttaaagtt tctctaataa cttgattact
    17341 gataccagct agtttagcaa ccgcaatagt tgctagagca ttctctacgt tatggcttcc
    17401 tgggacacca atgtcatcta ctgacataat attctcccct ttatagaaaa gttgcttgtc
    17461 ttgtacgtaa gcaccatcaa ctttttccgt agtagagaaa ggaacgattg ttgctttagt
    17521 agttttagct aactctttag aaataccttg attaaaatta agtaccaaaa aatcagatga
    17581 agacatttga ttttggatat tccattttgc agcaacataa tcttcaaaag acccatgata
    17641 atctaaatga gttggcatta aattagtaat tactgcaata tgaggacgaa attccttaac
    17701 tcccattagc tgaaaacttg ataattccat aactagaata tctttatcat ccgcagcctg
    17761 aacaacttca ctagcaggaa agccgatatt cccagctaac aaacctctct gacctccagc
    17821 atttaagact tctgcaatca tcgttgtcgt tgtcgttttc ccgttagagc ctgtaatacc
    17881 tattagctga gattctgaaa ctaagtatgc taattccact tcagtcaaaa cagggatttg
    17941 tttttctaat gcttttttga ccataggatt gttataaggt attcctggat ttttaatcat
    18001 gtaacaaaaa tcctcatcta acaattctaa aggatgacta ccacaaacca ctttaatacc
    18061 ctcttccaac aaagactgtg ctgttggatt ttcatcaaat ggtttgccat cattaactgt
    18121 cactattgct cctaacttag ctaacaaacg tgcagcggct tctccagatc gtgctaaacc
    18181 aaggactaaa acttttttat tttcaaatgt tgttattgtt ttcattactc gtcccattct
    18241 atcattatta tccctattat tttaccttta atcagtggct ttttcaaggt aaaggatatg
    18301 tctctaattt ctcaaaacaa aagaacttag gagacaaaat cctaagttca taagctattt
    18361 cttcttttct gacaaattca atgtttcttt aaatgctgta tatcctagag gaatcattat
    18421 aagcccaata tacaattcac ctggcaattg aaggatttta ataaaactca taactgccaa
    18481 gacaagtaat aaggctacca aacctactaa accaatagcg ttcaaagtat tacgtactgt
    18541 ttttggtgcc ataaaaaagt aatataaggc aatcaaaagg gcaacaatta agtaaaacat
    18601 agaactcctt tcttattaat cataaataag aaatactttg gatattattc agctgatttt
    18661 ttctttttat tagctttgtc acgtgctgct ttatttaaaa tttgtttacg aagacgaata
    18721 gactcaggag ttacttccat gtactcatca tctgctaaaa attcaagtga ctcttctaaa
    18781 gtaaggatac gaggcgtctt gatgactgca gtttgatctt tagttgctga acggacattt
    18841 gtcatttgtt tagcagttgt aatattgact ccgagatcat tatcacgaga attctcacca
    18901 acaatcattc cttcataaac ttctgtacct ggatttacaa agatagtccc acgttcttca
    18961 atacgcataa ttgaatatgt agttgcttta ccattttcaa tagaaaccaa gacaccacga
    19021 tgacgaccac caatttctcc ttgaacaacc ggtagatact ggtcaaaagt atgattcatg
    19081 ataccatatc cacgtgtcat tgaaagaaac tctgttgaat aaccaatcaa accacgtgca
    19141 ggaatcaaga aaatcaaacg cgtttgacca ttaccaacca tctgcatatc aagcatatca
    19201 cctttacgct ctgacaaact ttggataata gcaccctgat attcttctgg agtatcaatt
    19261 tgaacacgct caaacggctc gcattgaaca ccatcaattt ctttgatgat aacttctgga
    19321 cgtgatactt gaagttcata tccctcacga cgcattgttt caatgaggat agataaatgt
    19381 aattctccac gccctgaaac cgtccattta tctggcgaat ctgttgggtc aacacgcaaa
    19441 gaaacgtctg tttgtaattc tgctaaaaga cgttcttcaa ccttacgtga cgtaatccat
    19501 ttaccttcgc gacctgcaaa tggtgaatta ttcaccaaga aagtcatttg aagtgttggc
    19561 tcgtcaatac gtaaaactgg tagtggttca atagcatctg tcggagttac tgtctcacca
    19621 acgaagatat cttccatacc agaaacagcg attaaatcac ccgctttagc ctcttggatt
    19681 tctttacgtt caagtccaaa gaaaccaaaa agttttgtta cgcggaagtt tttagttgta
    19741 ccatcaagtt ttgaaagagt aacttgatct ccaactttga cagtcccgcg gaaaacacgt
    19801 ccaataccaa tacgtcctac aaaatcattg taatcaagaa gagaaacttg gaattgaagt
    19861 ggttcttccg agttgtcaac tggggctgga atgtgatcaa tgatagtatc aaaaatcggt
    19921 gccattgttt tttcttgatc tgaaggatca tctgacattg aagatgttcc attgatggct
    19981 gaagcataaa caacagggaa atctagttgg tcatcatcag caccgagctc aatgaagagt
    20041 tcaagaactt catcaacaac ctctgatgga cgagctgatg gcttatcaat tttatttaca
    20101 acaacgattg gaattaagtt ttgttcaaga gctttcttca aaacaaaacg tgtttgtggc
    20161 attgttcctt cgtaagcatc aacgactaaa acaacaccat caaccatttt cataatacgc
    20221 tcaacttcac caccaaagtc cgcgtgacca ggtgtgtcca taatattgat acgaacatcg
    20281 ttgtatgcta cggctgtatt ttttgcaaga atggtgatac cacgttcttt ctcgatatca
    20341 tttgaatcca ttgcacgttc ttcaagctct ttacgctcat caagagtatg tgattgtttt
    20401 aataattcat caacgagagt tgttttaccg tggtcaacgt gggcaatgat cgcaacgtta
    20461 cggatatctg ttcttaaatt tgtcatgttt tcctctattt tataatgtaa ttctgttaac
    20521 ttatgaatta taacacaatt tttcttaaaa aacagaattt acacaagtgt aaatgttttc
    20581 atattaactg cctatttgtg ataggaaaac ttttcatcat ttcattattt atgtcattta
    20641 ttttctactt ttttcaattt aattcatttc tacaaaaaga gaagagagtt attaatagta
    20701 actctctcct tagtatatta cttaactcta cccgtccagt aattgaaacc gtcctttaac
    20761 acgtaaagtt gattaaaacc ttcttttctg agtaacaaaa caatccttgg aatagactga
    20821 ccacggcttg cgtcatataa gaggacaggt ttatctttgc gtaaagccga taaggcaact
    20881 ttaaattgac ttgctggaat gttacgtgct ccgagaatat gctttctatg aaaagcacca
    20941 gcttctcgga tatcaattaa ctgtcctcgt gacatttctt tttgaaaaga ctcgttatct
    21001 aaaaatttag ctgccctacg aaccctccaa taattccaag atgcccaagc aacaaaagcc
    21061 agaagaataa cgataattaa aataacagac atatccatat cttatctcct taacttgctt
    21121 gttgattggc taagcttgct gcaccaataa taccagcatc attacctagt tcagcaatct
    21181 taattttagt tgacttttta acttgtggga aagcaaatgt gacaaagtat ttctcaacgc
    21241 gactacgtaa aaattcacct gctgctgaga caccgccacc aataaccaca gaatcagggt
    21301 ttaaaatatt tgaaatatta gctgctgcca gtccaaggta acgtgataca cgttcaacaa
    21361 cagaattagc aaatttatcc ccatcttctg ctgctataaa aatatcttta cttgtaacag
    21421 tatcaccgtt gtcaatcgct gctttaatgg cagacgaacc ttcatattgt tctgcgagtt
    21481 gacgtgctac tctaacaaca cctgtcgctg atgcaactgt ctcaagacag cctttgttac
    21541 cacatgtgca cgtaaatcca ttttctggat caacaatcat atgcccaatt tctccacctg
    21601 ctcctgcaac accatggatg aggttaccat ctgcgataac acctccacct actcctgttc
    21661 cgagggttac gaaaacaaca tcgggattat tggcaccagc acctacccag cgttcaccaa
    21721 gtgctgcaac attagcatcg ttatcaataa aaaatggaat tccaacttct ttttcaataa
    21781 ccgaacctac ttcttgagta tcagcccaat ttagattaaa agcacctgtt actgttttac
    21841 tagttctatc aacagctcct ggagaaccca taccgatacc gagaaagtca tcttttgtta
    21901 atccatagag gctcaaacga tgtttgagag attcaacgat atcagaaacg atatgtcttc
    21961 cgttttctaa agtattggtc tcaattgccc atttttcttg tacttctccc tcaagcgtca
    22021 agataccaaa tttaatggtc gttcctccga ggtcaattcc caataatttc ttactcattt
    22081 tttattttcc ttctccaatt ctagtctgtg ctctcttctt aaaatcagtt ctgcttttaa
    22141 atagtcatct ctacttatca gaccattatc gtagagacgt tgcaattcaa tcttcataac
    22201 ttcgatatca taaagacgtt taccaatata aacataaata ccaaattttt tcaaaagatt
    22261 ctgcacatcg aataaaactt tcatatatga attttaacag aaaaccattc aattttcaag
    22321 aaaatccgca accgcttgct aattgttatt atgacgttag gctgactatc tataatagaa
    22381 atcataatct cttttctacc atttttcgaa tataaatata aactataaaa aggat
//
LOCUS       DATROD010000019        21934 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.019, whole genome shotgun sequence.
ACCESSION   DATROD010000019 DATROD010000000
VERSION     DATROD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21934
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.019"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..139
                     /locus_tag="VBC74_000758"
     CDS             <1..139
                     /locus_tag="VBC74_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEO6941728.1"
                     /translation="GYVVDMVHLDFINFAIFNVADSYLTIGIICLMIALWKEESNGNH
                     N"
     gene            123..1013
                     /locus_tag="VBC74_000759"
     CDS             123..1013
                     /locus_tag="VBC74_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEO6941729.1"
                     /translation="MEITIKIAGVRLDKALADNSELSRSQANEEIKKGIVLVNGQVKK
                     AKYTVQEGDRITFDIPKEEVLDYQAENIPLDIIYQDDDVAVVNKPQGMVVHPSAGHSS
                     GTLVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKNDRAHQVLAEELKDKKS
                     LRKYLAIVHGNLPNDRGVIEAPIGRSDKDRKKQAVTAKGKPAITRFHVLERFGDYTLV
                     ELSLETGRTHQIRVHMAYIGHPLAGDPVYGPRKTLGGKGQFLHAQTLGFTHPSNGENL
                     IFSVEVPEIFQTTLDKLRKN"
     gene            1189..1710
                     /gene="pyrR"
                     /locus_tag="VBC74_000760"
     CDS             1189..1710
                     /gene="pyrR"
                     /locus_tag="VBC74_000760"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEO6941730.1"
                     /translation="MKRKEIIDDVTMKRAITRITYEIIERNKNLDNIVLAGIKTRGVF
                     LAKRIQERLKQLENLDIPVGELDTKPFRDDMKVEVDTTTMPVDITDKDIILIDDVLYT
                     GRTIRAAIDNLVSLGRPSRVSLAVLIDRGHRELPIRADYVGKNIPTSQFEEILVEVME
                     HDGYDRVSIIDPS"
     gene            1759..2829
                     /locus_tag="VBC74_000761"
     CDS             1759..2829
                     /locus_tag="VBC74_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEO6941731.1"
                     /translation="MAKKLLILEDGTVFEGLSFGSSLDVTGELVFCTGNTGYQEIITN
                     PSHNGKILVFTSPLIGNYGIHRSYSEAIIPTCLGVVVAEYSRCVSSDTSKMNLDEFLK
                     MKKVPAMSGVDTRYLMQVIKEKGFVKATLAEAGDVLSHLQDQLIATVLPTNNVEQVST
                     KTAYPSPASGRNIVVLDFGLKHSVLRELSKRQCDVTVIPYNTSLEGIKNLYPEGIILS
                     NGPGNPEKLQEVLNTIKELQKSVPMLGIGLGHQLIAMANGAEIMRLPVAKKGPNYPMR
                     DIATGRLETVIQFNHFTVNRLNLPHDLLVTHEGLNDQEIVALRHRSFPVMSVQFYPEA
                     APGPHDVTYFFDEFLEMIGSRA"
     gene            2885..4483
                     /locus_tag="VBC74_000762"
     CDS             2885..4483
                     /locus_tag="VBC74_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEO6941732.1"
                     /translation="MLNTKDKQPILLIIFGQKMKEYDDLIRSTVERLSKRNRPVSILI
                     SKELNHVIHHYDAQFENLETLVGYINTHEDSLIYPLCLDKNHELFQEFLAWQSHYSRQ
                     YISDNLSEKSVQAIDNNQDFVQVDCLVMRDSLNNCLYVSDLEYIESNKTTGKSLAIVP
                     SQTLSDAARQTIRDVAFDVCRKANIIGVCYFSFLIDLNSLDYHIISLSSGLSYQSILF
                     ETITTYPVLEIATKLTVGYTFSQLKHSYYPNTSAFLEPQLDYVATVSFSFEKVDYIFF
                     ARNIEQLFLNLLEASSHDHFPFLSDISEEDLMFALIQKKENRLAYLLEAFRRGFDLYD
                     LSSVTKINPFYLDKCLHIVELYENLNKSQYNVDIYKDAKRYGFSDDYIASSWQISLID
                     MLEYRKKHSVAPVLKQVEQSSGVLTGHQIQYFRSYDWHSDYSSSGCQKALIMVDKGYS
                     LVKLNELIKQIKQTHLELLIVTNQPLLIEQLNDTSIIFDTIGIETILTIMGIEEVEEV
                     YLLGESQADMFSKMKNFSISCQSI"
     gene            4685..5929
                     /locus_tag="VBC74_000763"
     CDS             4685..5929
                     /locus_tag="VBC74_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEO6941733.1"
                     /translation="MSKRQNLGISKKGAIISGLSVALIVAVGGFLWVQSQPNKSAVKT
                     NYKVFNVREGSVSSSTLLTGKAKANQEQYVYFDANKGNRATVTVKVGDKITAGQQLVQ
                     YDTTTAQAAYDTANRQLNKVARQINNLKTTGSFPAMESSDQSSSSSQGQGTQSTSGAT
                     NRLQQNYQSQANASYNQQLQDLNDAYADAQAEVNKAQKALNDTVITSDVSGTVVEVNS
                     DIDPASKTSQVLVHVATEGKLQVQGTMSEYDLANVKKDQAVKIKSKVYPDKEWEGKIS
                     YISNYPEAEANNNDSNNGSSAVNYKYKVDITSPLDALKQGFTVSVEVVNGDKHLIVPT
                     SSVINKDNKHFVWVYNDSNRKISKVEVKIGKADAKTQEILSGLKAGQIVVTNPSKTFK
                     DGQKIDNIESIDLKSNKKSEVK"
     gene            5932..6642
                     /locus_tag="VBC74_000764"
     CDS             5932..6642
                     /locus_tag="VBC74_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6941734.1"
                     /translation="MTTNRKELIRLHQIVKSYQNGDQKLQVLKNIDLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDSPTSGDYSLNGKRVEELSQTKLAQVRNKEIGFVFQQFFLL
                     SKLTALQNVELPLIYAGVAPKKRKNLAKQFLDKVELRERMNHLPTELSGGQKQRVAIA
                     RALVNSPSIILADEPTGALDTKTGEQIMQFLTELNQEGKTIIMVTHEPEIADYATRKI
                     VIRDGEITADTTDSIRID"
     gene            6657..7904
                     /locus_tag="VBC74_000765"
     CDS             6657..7904
                     /locus_tag="VBC74_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6941735.1"
                     /translation="MENWKFALSSILGHKMRAFLTMLGIIIGVASVVLIMALGKGMKD
                     SVTNEITKSQKNLQIYYKTKEDQKNEDNFGAQGAFMQGSDTNRKEPIIQESWLKKIAK
                     EVDGVSGYYVTNQTNAPVAYLEKKAKTVNITGINRTYLGIKKFKIKSGRQFQEEDYNQ
                     FSRVILLEEKLAQRLFQTNEAALNKVVTVKNKSYLVVGVYSDPEAGSGLYGSNSDGNA
                     ILTNTQLASEFGAKEAENIYFHLNDVSQSNRIGKEIGKRLTDISHAKDGYYDNFDMTS
                     IVKSINTQVGIMTGVIGAIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRRK
                     ILAQFLIESMVLTILGGLIGLLLAYGGTMLIANAQDKITPSVSLNVAIGSLIFSAFIG
                     IIFGLLPANKASKLNPIDALRYE"
     gene            8033..8305
                     /gene="rpsP"
                     /locus_tag="VBC74_000766"
     CDS             8033..8305
                     /gene="rpsP"
                     /locus_tag="VBC74_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEO6941736.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQVTIKEERVLEWLSKGAQPSDTVRNLLSKAGVMTKFHDQKFSK"
     gene            8315..8557
                     /locus_tag="VBC74_000767"
     CDS             8315..8557
                     /locus_tag="VBC74_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEO6941737.1"
                     /translation="MDTIENLIIAIVKPLISQPDQLTIKIQDGPEFLEYHLDLDTQDI
                     GRVIGKKGRTITAIRSIVYSVPTQGKKVRLIIDEKE"
     gene            8689..10200
                     /locus_tag="VBC74_000768"
     CDS             8689..10200
                     /locus_tag="VBC74_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6941738.1"
                     /translation="MLFDFLEEKIKLKAYLMTALLDDGEINLDHFKTEYALSEKILQG
                     LISELQILYSDFYLVVKSRTMVLFHYETLSKLETLHTIYRESNTLQFLAYFIAPQNVS
                     FSEFTAKKFISVASAYRIKKNCKLYLESVKLSIQKNKVTGPEYQIRLLIALLQYHFGF
                     IIYPFDATSEVMIRDFIEYSNFKGCDYDLKDLSEEYYYFYILVNLVWKRKEYKVEIPQ
                     TESFNQLKKSILYMELSKAAQKSIAIHLGIELNEGLLDYLYLVFCISDGPLFSNRDDI
                     NINEEFIFASYKISKSKAFKNFCGRLLGREFIDSQHFKDINAYFLRHFICYCYYFIPD
                     FYFLNTSRLSYSKDLYHLLDKGLADIFNLKGGNLTFSKHETVLLTMQLSNLIETFLAP
                     LSVYVISSSNIRLQTYQVMLNQYFTSKIAEFFFVNYQTTQIDEKLLKKADIIIAERRY
                     ISSLKNDSGVAIQDILEIGMNNKQYHELLLQAIIEKRDQKVFEYISKMKHKIL"
     gene            10308..10379
                     /locus_tag="VBC74_000769"
     tRNA            10308..10379
                     /locus_tag="VBC74_000769"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10340..10342,aa:Gln,seq:ttg)
     gene            10388..10459
                     /locus_tag="VBC74_000770"
     tRNA            10388..10459
                     /locus_tag="VBC74_000770"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10421..10423,aa:Glu,seq:ttc)
     gene            10522..11040
                     /gene="rimM"
                     /locus_tag="VBC74_000771"
     CDS             10522..11040
                     /gene="rimM"
                     /locus_tag="VBC74_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEO6941739.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVTDFVEERFKKGQVLALFDEK
                     NQFVMDIEIASHRKQKNFDIIKFKGMYHINDIEKYKGFTLKVAEDQLSDLKDGEFYYH
                     EIIGLDVYEGEELIGKIKEILQPGANDVWVVERHGKRDLLLPYIPPVVLEVNLSNQRV
                     QVELMEGLDDED"
     gene            11027..11782
                     /gene="trmD"
                     /locus_tag="VBC74_000772"
     CDS             11027..11782
                     /gene="trmD"
                     /locus_tag="VBC74_000772"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEO6941740.1"
                     /translation="MMKIDILTLFPEMFAPLEHSIVGKAKERGLLEINYHNFRENAEK
                     SRHVDDEPYGGGQGMLLRAQPIFDTIDKIDAQKARVILLDPAGRTFDQDFAEELSKED
                     ELIFICGHYEGYDERIKSLVTDEVSLGDFVLTGGELAAMTMVDATVRLIPEVIGKETS
                     HQDDSFSSGLLEYPQYTRPYDYLGMTVPDVLMSGHHENIRKWRLEQSLRKTLERRPDL
                     LENYAMTDEERLILEKIKTEIERTDTVNEQNNL"
     gene            11763..12755
                     /locus_tag="VBC74_000773"
     CDS             11763..12755
                     /locus_tag="VBC74_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEO6941741.1"
                     /translation="MNKTIYDITIVGGGPVGLFAAFYAGLRGVSVKIIESLSELGGQP
                     AILYPEKKIYDIPGYPVITGRELIDKHIEQLERFKDSIEICLKEEVLSFEKVDDVFTI
                     QTDKDQHLSRAIVFACGNGAFAPRLLGLENEENYADNNLFYNVTKLEQFAGKHVVICG
                     GGDSAVDWANELDKIAASVAIVHRRDAFRAHEHSVDILKASGVRILTPYVPIGLNGDS
                     QRVSSLVVQKVKGDEVIELPLDNLIVSFGFSTSNKNLRYWNLDYKRSSINVSSLFETT
                     QEGVYAIGDAANYPGKVELIATGYGEAPVAINQAINYIYPDRDNRVVHSTSLIK"
     gene            12892..13962
                     /locus_tag="VBC74_000774"
     CDS             12892..13962
                     /locus_tag="VBC74_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEO6941742.1"
                     /translation="MTNTVTPKETAGSFINKVLGGTATAIVVALIPNAILATFLKPFL
                     SYGLAAEFLHIVQVFQFFTPIMAGFLIGQQFKFTPMQQLAVGGAAYIGSGAWAYTEVI
                     QKGVATGSFQLRGIGDLINMMLTAALAVLAVKWFGNKFGSLTIILLPIIIGTGVGYLG
                     WKLLPYVSYVTTLIGQGINSFTTLQPIAMSILIAMAFSMLIVSPISTVAIGLAIGLNG
                     MSASAASMGVASTTAVLVWATMKANKSGVPIAIALGAMKMMMPNFLKHPVMAIPMLMT
                     ATVSSLTVPLFKLVGTPASSGFGLVGAVGPIASFEAGASMLIVILSWLVIPFAVGFVS
                     HKICKDILKLYKDDIFVFEGQN"
     gene            13975..14898
                     /locus_tag="VBC74_000775"
     CDS             13975..14898
                     /locus_tag="VBC74_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEO6941743.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNHDVILLDNWADHIMAIKENGL
                     KVTGDTEDLVKLPIMKPTDATEEADLIILFTKAMQLPNMLQDIKKIIGKKTKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLKGPGHAHLEGVGSVNLQSIDPNNQEAGHRV
                     TELLNEAKLQATYDENVLPNIWRKACVNGTMNSTCALLDCTIGQLFASEDGVNMVHEI
                     IHEFVTVGKAEGVELDEEEITKYVMDTSVKAAHHYPSMHQDLVQNQRLTEIDFLNGAV
                     NKKGENLGIDTPYCRLITQLIHTKENVLSIK"
     gene            15110..16744
                     /locus_tag="VBC74_000776"
     CDS             15110..16744
                     /locus_tag="VBC74_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEO6941744.1"
                     /translation="MMKKGQVNDTKQSYSLRKYKFGLASVILGSFIMVTSPVFADQTT
                     SVQVNNQTGTSVDANNSSNETSASSVITSNNDSVQASDKVVNSQNTATKDITTPLVET
                     KPMVEKTLPEQGNYVYSKETEVKNTPSKSAPVAFYAKKGDKVFYDQVFNKDNVKWISY
                     KSFGGVRRYAAIESLDPSGGSETKAPTPVTNSGSNNQEKIATQGNYTFSHKVEVKNEA
                     KVASPTQFTLDKGDRIFYDQILTIEGNQWLSYKSFNGVRRFVLLGKASSVEKTEDKEK
                     VSPQPQARITKTGRLTISNETTTGFDILITNIKDDNGIAAVKVPVWTEQGGQDDIKWY
                     TAVTTGDGNYKVAVSFADHKNEKGLYNIHLYYQEASGTLVGVTGTKVTVAGTNSSQEP
                     IENGLPKTGVYNIIGSTEVKNEAKISSQTQFTLEKGDKINYDQVLTADGYQWISYKSY
                     SGVRRYIPVKKLTTSSEKAKDEATKPTSYPNLPKTGTYTFTKTVDVKSQPKVSSPVEF
                     NFQKGEKIHYDQVLVVDGHQWISYKSYSGIRRYIEI"
     gene            16888..17973
                     /locus_tag="VBC74_000777"
     CDS             16888..17973
                     /locus_tag="VBC74_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6941745.1"
                     /translation="MTLRELTIEEFKEHSGNYDSQSFLQTPEMAKLLEKRGYDVRYLG
                     YQVENKLEIISLSYIMPVTGGFQMKIDSGPVHSNSKYLKQFYKALQGYAKSNGVLELI
                     VEPFDDYQLFTSSGVPSNQGNDNLIEDFTSSGYHHDGLTTGFTGKYLSWHYVKNLEGV
                     TSETLLSSFSKTGRALVKKAMSFGIKVRVLKRDELHLFKEITTSTSNRRDYMDKSLDY
                     YQDFYDSFEGKAEFVIATLNFREYDHNLQIKAEALENKLKLLDERFRENADSPKYHRQ
                     RSEIINQLASFETRRQEVQSFIQKYDNQDVVLAGSLFVYSLKETVYFFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRGSTFYNLLGI"
     gene            17998..18123
                     /locus_tag="VBC74_000778"
     CDS             17998..18123
                     /locus_tag="VBC74_000778"
                     /inference="COORDINATES: protein motif:HMM:NF014445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEO6941746.1"
                     /translation="MLRFKQNFNGYIIRKMGTFNYYPSPFKYKGIQLLKKVLKRN"
     gene            18188..18997
                     /locus_tag="VBC74_000779"
     CDS             18188..18997
                     /locus_tag="VBC74_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO6941747.1"
                     /translation="MLDIERKRDIIKLQTISIQGGTMLKSKRKEIILSRLEQNKSVTL
                     DELTSILETSESTVRRDLDELESEGFLKRVHGGAELPYSLGQELSNQEKSIKNVQKKL
                     DIARQTAKLIAKQDVIFIDAGTTTELLIDFLPHEQLTVVTNSIHHAAKLVDRGIKTII
                     IGGAVKHSTDASIGQVAINQIRQITVDKAFLGMNGIDEVYLTTPDLEEAAIKEAIINN
                     SQQTFILMDSSKIGQVTFAKVKEINDINLVTNKTDSELMTIIKEKMKVIQV"
     gene            18994..19905
                     /gene="pfkB"
                     /locus_tag="VBC74_000780"
     CDS             18994..19905
                     /gene="pfkB"
                     /locus_tag="VBC74_000780"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEO6941748.1"
                     /translation="MIYTVTLNPSIDFIVRLDTLLLGSVNRMTSDDKYVGGKGINVSR
                     ILKRLKIDNTATGFIGGFTGHFVEDGLVSEGIKTDFVSVNEDTRINVKVKAKIETEIN
                     GGGPRITNEQLHRLEKLLSRLTPEDTVVFAGSAPASLGNKVYNTLIPIAKKTGAEVVC
                     DFEGQTLLDALAYQPLLVKPNNHELADIFGVELEGLPDIEKYAHKILDKGAKNVIVSM
                     AGDGALLVTPEASYFAKPIKGEVKNSVGAGDSMVAGFTGEFVKSKNPVEALKWGVACG
                     TATTFSDDLATAEFIQDIYNKVEVEKL"
     gene            19902..21866
                     /locus_tag="VBC74_000781"
     CDS             19902..21866
                     /locus_tag="VBC74_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6941749.1"
                     /translation="MKIQDLLKKEVMIMDLKATSKEAAIDEMITKLVDTGVVTNFAIF
                     KDGIMKREAQTSTGLGDGIAMPHSKNAAVKEATVLFAKSASGVDYEALDGQPTDLFFM
                     IAAPDGANDTHLAALAELSKYLLKEGFADQLRQAKTPDDIIATFDSNSISQETVAPQT
                     VQSTSKGSDYIVAVTACTTGIAHTYMAEEALKKKAAEMGVGIKVETNGASGVGNKLTS
                     SDIARAKGVIIAADKAVEMDRFDGKPLVSRPVADGIKKSEDLINIILDNKAQTYHAKN
                     QNDKQSGESDGKSGLGSAFYKHLMGGVSQMLPFVIGGGIMIAIAFLFDNILGVPKDQL
                     SNLGSYHEIAALFKNIGGAAFAFMLPVLAGYIAYSIAEKPGLVAGFVAGSIASSGLAF
                     GKVPFAEGGKATLALAGVPSGFLGALVGGFLAGGVILLLRKLLSGLPKSLEGIKSILL
                     YPLLGVLITGFLMLLVNIPMAAINTALNTFLQGLSGSSAVLMGLLVGGMMAVDMGGPV
                     NKAAYVFGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFNNEERQSGL
                     TNIVMGLSFITEGAIPFGAADPARAIPSFIVGSALTGALVGLAGIKLMAPHGGIFVIA
                     LTSNPLLYILFILIGAIVSGVLFGLFRKTK"
BASE COUNT         7379 a         3271 c         4370 g         6914 t
ORIGIN      
        1 aggttatgtc gttgatatgg tgcatttaga ttttattaat tttgctatct ttaatgtagc
       61 agactcatat ctgacaatcg gtattatttg cttaatgatt gcactttgga aagaggaaag
      121 taatggaaat cacaattaaa atagcaggag ttaggctaga taaagcttta gctgataatt
      181 ctgaactgtc gcgtagtcaa gctaatgaag aaattaaaaa agggattgtg ttagtaaatg
      241 gacaagtaaa aaaagcgaaa tatactgttc aagaaggaga tcggattact tttgatattc
      301 ctaaagagga agtactagat tatcaagctg aaaatatccc tttagatatt atctatcaag
      361 atgatgacgt tgctgtggtt aataagccgc aagggatggt tgttcaccct tctgcaggcc
      421 acagttcggg caccttagtt aacgccttga tgtatcatat taaggattta tcatcaatta
      481 atggcgttgt tcgcccaggg atcgttcatc gtattgataa agatacttct ggtttattga
      541 tggttgcaaa aaatgataga gctcaccaag tgttggctga agagttgaaa gataaaaaat
      601 cgcttcgtaa atatttagca attgtgcatg gtaatttacc aaatgatcga ggcgtgattg
      661 aggctccgat tggtagaagt gataaagatc gtaagaagca agccgtgact gctaaaggta
      721 agccagcaat aactcgtttt catgttttag aacgttttgg tgattatacc ttagttgaat
      781 tgtcattgga gacaggtcga acgcaccaaa ttcgggttca tatggcttat attggccatc
      841 ctctagccgg tgatcctgtc tacggtcccc gcaagacttt agggggtaaa ggacagtttt
      901 tgcatgcaca aacactaggt tttacgcacc ctagtaacgg agagaatttg attttttcag
      961 tagaggttcc ggaaattttt cagacaactt tagacaagtt gagaaaaaat taagtttttc
     1021 ttgaaaaata cttttaccat ttgctataat ataagcaaat cattccttta accttatcca
     1081 gtgagatagt taaggagaaa cttcagacta aatcaatgcc tgagcggctt tgtcattcta
     1141 gagagtctcc ttttgaaggg gctcttttat cttagagagg atactgcaat gaaaagaaaa
     1201 gaaattattg atgatgtgac catgaaacgt gctattacac gtattaccta cgaaattata
     1261 gaacgtaaca aaaatttaga taatattgtt ttagcaggca ttaaaacaag aggtgttttt
     1321 ttagccaaac gcattcaaga acgtttgaag caattagaaa atttagatat tcccgttgga
     1381 gaattggata ctaaaccttt ccgtgatgat atgaaagttg aagttgacac cacaactatg
     1441 ccagttgaca tcactgataa agatattata ttaattgatg atgttctcta tacaggtcgc
     1501 actattcgag ctgctattga taatcttgtt agtttggggc gtccgagtcg tgtcagttta
     1561 gctgtattaa ttgaccgtgg gcatcgtgaa ctacccattc gtgcagatta tgtggggaaa
     1621 aatattccaa caagtcaatt tgaagaaatt ctagttgaag tgatggaaca tgatggttac
     1681 gatcgtgtga gtattattga tccgagttaa gtagacgact attatttacg atgattttat
     1741 agaaggggaa gaaccacaat ggcaaaaaaa ttgttaattc tagaggatgg tacggttttt
     1801 gaaggactat cctttggttc atctcttgat gtaactggcg aattagtttt ttgtacgggg
     1861 aataccggct atcaggaaat aataacgaat ccttcacata atggaaaaat tttagttttt
     1921 acctcaccgc taataggaaa ttatggtatt catcgtagtt actctgaagc cattattccc
     1981 acttgtttag gagttgttgt tgcagagtat tccagatgtg taagtagcga tactagcaag
     2041 atgaatctag atgaattttt gaaaatgaaa aaggttcctg caatgtcggg ggtcgataca
     2101 aggtatttga tgcaagttat taaagaaaaa ggatttgtaa aagctacttt agcagaagca
     2161 ggtgatgttt tatcacattt acaagatcaa ttaatagcga cagtattacc gacaaataat
     2221 gtggaacaag tttcgacaaa aacagcttat ccgtcgcctg ctagcggtcg aaatattgtt
     2281 gtacttgatt ttggccttaa acattctgtt ttgagagaat tgtctaaacg tcagtgtgac
     2341 gtgactgtca ttccctacaa tacgtcttta gaaggtatca aaaatctata cccagaaggt
     2401 atcatattat ctaatggtcc gggaaatcca gaaaagcttc aggaagtctt aaacactatt
     2461 aaagaattac aaaaatctgt tcctatgtta ggtattggtt tggggcatca attaattgcc
     2521 atggcaaatg gtgcagaaat tatgagactc ccagttgcta agaaggggcc aaattatccc
     2581 atgagagata ttgctacggg gcgtttagaa acagttattc agtttaatca ttttaccgtt
     2641 aaccggctaa atcttccgca tgatttactg gtaacacacg aaggtttgaa tgatcaagag
     2701 attgttgctc taaggcaccg ctcatttcca gtgatgtccg tgcaatttta cccagaggct
     2761 gctccaggcc ctcatgatgt gacctatttt tttgatgaat ttttagaaat gattggatca
     2821 cgcgcttaaa cataaggtat tatttaaagt cagtcggtga gctgattaat aaggaggtgc
     2881 gtatatgcta aatacaaagg ataaacagcc tattttgctt attattttcg gacagaaaat
     2941 gaaagaatat gacgatttaa ttagatcaac tgttgagagg ttatctaaaa ggaatagacc
     3001 tgttagtatt cttatctcga aggagctaaa ccatgtgatt caccattatg atgcacaatt
     3061 tgaaaatctt gagacgcttg tggggtatat aaatacacat gaagatagtt tgatttatcc
     3121 cctttgctta gataaaaatc atgagctatt tcaagagttt ttagcgtggc agagtcatta
     3181 ttcaaggcag tatatcagtg ataatttatc agaaaaaagt gttcaggcaa tagacaataa
     3241 tcaagacttt gtgcaagtag attgtttagt aatgagagac tctttgaata attgtttata
     3301 tgtctctgat ttagaatata ttgaatctaa taaaacaact ggtaagtctc tagcgattgt
     3361 accaagtcaa actttatccg atgctgctag gcaaacaatt cgagatgttg catttgatgt
     3421 gtgtcgaaag gcaaacatta ttggagtatg ttactttagt ttcttaatag atttgaacag
     3481 tttggattat catataatta gcttatcatc tggtttgtct tatcaaagca ttttatttga
     3541 gactattacg acctatcctg ttttagagat agcaactaaa ttgacagtgg gatatacttt
     3601 tagtcaactg aagcattctt attacccaaa tacctctgcc tttttagagc cgcaattaga
     3661 ctatgtagct accgtatctt ttagttttga aaaagttgac tatatctttt ttgctcgtaa
     3721 tatagagcaa ttatttttaa atttactcga ggctagcagt catgatcatt ttccatttct
     3781 gagtgatatt agcgaggaag atttgatgtt tgctttgatt cagaaaaaag aaaatcgtct
     3841 agcttatctt ttagaggctt ttagacgtgg ctttgatttg tatgatttat cctcagttac
     3901 taaaatcaac cctttttact tagataaatg tttacatatt gtagagttgt atgaaaacct
     3961 gaataaaagc caatataatg tagatattta caaggatgct aaaaggtatg gtttttcgga
     4021 tgactatatt gctagtagtt ggcaaatatc tttgatagac atgttagaat atcgaaaaaa
     4081 acatagtgtc gcacctgttc tgaagcaagt cgaacaaagt tctggtgttc taactggcca
     4141 tcaaattcag tattttcgaa gctatgattg gcattcagac tacagttcta gcggttgtca
     4201 aaaggctttg ataatggtag acaagggtta ttccctagta aaattaaatg agttaattaa
     4261 gcaaatcaaa caaactcatt tagaattatt aattgttacc aatcaacctc tcctcataga
     4321 acaattaaat gatacatcta taatttttga cactataggt attgagacca tattaacaat
     4381 tatgggtata gaagaggtgg aagaagtgta tttattggga gaaagtcaag cagacatgtt
     4441 ttcaaaaatg aaaaattttt ctatctcttg tcagtctatt tagcaattct taataaaagc
     4501 tgagatatag atctcagctt ttatttgtgg taaaaagtaa agtactttca attttgtaag
     4561 ttctttctat gagcaaaata gtatacttaa tgagtaaaaa tattattttt aaagaatttt
     4621 cttgacaata ttatttaaaa ataatacact tgttctatat taataataca aacaggagat
     4681 ggacatgagt aaacgacaaa atttagggat tagtaaaaaa ggagcaatta tatcagggct
     4741 ctcagtggca ctaattgtag cagtaggtgg ctttttatgg gtacaatctc aacctaataa
     4801 gagtgcagta aaaactaatt acaaagtttt taatgttaga gaaggaagtg tttcgtcctc
     4861 aactcttttg acaggaaaag ctaaggctaa tcaagaacag tatgtgtatt ttgatgctaa
     4921 taaaggtaat cgagcaactg ttacagttaa agtgggtgat aaaatcacag ctggtcagca
     4981 gttagttcaa tatgatacaa caactgcaca agcagcctac gacactgcta atcgtcaatt
     5041 aaataaagta gcgcgtcaga ttaataatct aaagacaaca gggagttttc cagctatgga
     5101 atcaagtgat caatcttcat catcatcaca aggacaaggg actcaatcga ctagtggtgc
     5161 gacgaatcgt ctacagcaaa attatcaaag tcaagctaat gcttcataca accaacaact
     5221 tcaagatttg aatgatgctt atgcagatgc acaggcagaa gtaaataaag cacaaaaagc
     5281 attgaatgat actgttatta caagtgacgt atcagggaca gttgttgaag ttaatagtga
     5341 tattgatcca gcttcaaaaa ctagtcaagt acttgtccat gtagcaactg aaggtaaact
     5401 ccaagtacaa ggaacgatga gtgagtatga tttggctaat gttaaaaaag atcaggctgt
     5461 taaaataaaa tctaaggtct atcctgacaa ggaatgggaa ggtaaaattt catatatctc
     5521 aaattatcca gaagcagaag caaacaacaa tgactctaat aacggctcta gtgctgtaaa
     5581 ttataaatat aaagtagata ttactagccc tctcgatgca ttaaaacaag gttttaccgt
     5641 atcagttgaa gtagttaatg gagataagca ccttattgtc cctacaagtt ctgtgataaa
     5701 caaagataat aaacactttg tttgggtata caatgattct aatcgtaaaa tttccaaagt
     5761 tgaagtcaaa attggtaaag ctgatgctaa gacacaagaa attttatcag gtttgaaagc
     5821 aggacaaatc gtggttacta atccaagcaa aactttcaag gatgggcaaa aaattgataa
     5881 tattgaatca atcgatctta agtctaataa gaaatcagag gtgaaataat cgtgacaact
     5941 aatcgaaaag aactgattag attgcatcag attgttaaaa gttatcaaaa tggggatcaa
     6001 aaacttcaag tcttaaagaa cattgatttg acggtttatg aaggtgaatt tcttgcaatc
     6061 atggggccat ctggctcagg gaaatccaca ctaatgaata ttattggctt gttggattcc
     6121 ccaacttcag gagattatag tttaaatggt aaacgtgtcg aagaactaag tcaaacaaaa
     6181 ttagctcaag ttcgaaataa ggaaatcggt tttgtctttc aacaattttt cttgttatct
     6241 aaactaacgg ctttgcaaaa tgttgaatta cccttaattt atgcgggtgt agctcctaaa
     6301 aagcgtaaaa atttagcaaa acaattttta gataaagttg aattgagaga gcgtatgaac
     6361 catctgccaa cagaattatc tgggggacag aagcaacgtg tagcaatcgc gcgtgctttg
     6421 gtgaatagcc catctattat tctagcagat gagccgactg gtgccttgga tacaaagacg
     6481 ggtgagcaaa ttatgcagtt cctaacggaa ttaaatcaag aaggtaaaac cattatcatg
     6541 gtaactcatg aaccagaaat cgccgattat gcgacacgta aaattgttat tcgtgatggc
     6601 gaaattacag cagatactac tgatagtatt agaattgatt aaggggagat tcgattatgg
     6661 aaaattggaa atttgcccta tcatcgattc tagggcacaa gatgcgtgct tttctgacca
     6721 tgttagggat tatcattggg gttgcatcag ttgtgttaat tatggcctta ggtaagggca
     6781 tgaaagattc ggttacaaat gagattacaa agtctcagaa gaatcttcaa atttattata
     6841 aaacaaaaga agaccaaaaa aatgaggata actttggagc tcaaggggct ttcatgcaag
     6901 gtagtgatac caaccgtaag gaaccaatta ttcaagaatc atggttaaaa aagattgcta
     6961 aggaagtaga tggtgttagt ggctactatg taaccaatca aacaaatgct ccagtagctt
     7021 accttgaaaa aaaggcaaag acggttaata taacaggaat taaccgcact taccttggta
     7081 tcaaaaagtt caaaattaaa agtggtcgtc aattccaaga ggaggattat aaccaattct
     7141 cgagagttat tttactagaa gaaaaattag ctcagagatt atttcagaca aatgaagcag
     7201 cgttaaataa agtggtgact gtaaaaaata aatcttactt agtagtgggg gtttattcgg
     7261 atccagaagc tggctcaggt ttatatggaa gtaattctga tgggaatgct attttaacca
     7321 atacacagtt agcttcggag tttggtgcta aagaagcaga aaacatatat tttcatctta
     7381 atgatgtgtc gcaatccaat cgcattggta aagaaatagg caaacgttta acagacatta
     7441 gccatgctaa ggatgggtac tatgataatt ttgatatgac atctatagtt aaatctatca
     7501 atacccaagt tggtattatg acaggtgtta ttggagcgat tgcagcaatt tctctcctag
     7561 ttggtggtat cggagtgatg aatattatgt tagtctctgt aactgagcgc acgagagaaa
     7621 ttggccttcg taaagccctt ggggcaactc gacgtaaaat tttagctcaa tttttaattg
     7681 aatcaatggt cttaacgatt ttgggtgggt taattgggtt attactagcc tatggaggta
     7741 cgatgttaat tgctaatgcg caagataaga ttactccttc tgtgtcactt aacgtggcta
     7801 taggcagttt aattttctca gcatttattg gaattatatt tggtttatta cctgctaata
     7861 aagctagcaa attaaaccca attgatgctt tacgatatga ataaaaaaac atgtactctg
     7921 tcaagatttt ttcttgacag agtttgtatt tatgttatac ttagtaaggt aaagttgtta
     7981 gttaagctat caacgaccaa atataaagaa aaagaaaaga gactatttaa aaatggcagt
     8041 aaaaatccgt ttaactcgta tgggttctaa gaagaaacct ttctaccgta tcaatgttgc
     8101 agattcacgt gcaccacgtg acggacgttt tattgaaact gttggaacat acaacccatt
     8161 agttgcagaa aaccaagtta caatcaaaga agaacgtgtt cttgaatggt tatcaaaagg
     8221 tgcacaacct tcagatacag ttcgtaacct tctttcaaaa gctggagtta tgactaaatt
     8281 ccacgatcaa aaattctcaa aataataaaa gcctatggat accattgaaa atctcattat
     8341 tgctattgtg aaacctttga tttcacaacc agatcagtta accataaaaa ttcaggatgg
     8401 cccagaattt ttagagtacc atcttgattt agatacccaa gacatcggtc gtgttatcgg
     8461 caaaaagggt cgtacaatta cagcgataag gtcgattgtc tattcggtac caacacaagg
     8521 gaaaaaagta agactcatca ttgatgagaa agaataattt attctaaaag ataggaactt
     8581 cctgtctttt ttgttatatc tacacaaaaa ttgtgagttt tttagtcttt tagaatataa
     8641 tggatgtgaa agggaatttt ctgatttttt attaaggagg ataagatatt gctatttgat
     8701 tttctcgaag aaaaaataaa actgaaagca tacctgatga ccgccttact tgatgatgga
     8761 gaaattaacc ttgatcactt taaaacagaa tatgctttga gtgaaaagat tttacaaggt
     8821 cttatttcgg aactacaaat actttattct gatttctacc ttgttgtcaa aagtagaaca
     8881 atggtattgt tccattacga aacactttct aagctagaga ctttgcatac catttatcga
     8941 gagtctaata cgttacagtt tttggcatat tttattgctc ctcaaaatgt cagtttttct
     9001 gaattcacgg ctaagaaatt catatccgtc gcaagtgcct accgaataaa gaaaaactgt
     9061 aaactttatt tggagagtgt taagttatca atacaaaaaa ataaagtgac aggtccagaa
     9121 tatcagatta ggctgctgat tgctttgtta cagtatcatt ttggttttat tatttatcct
     9181 tttgatgcta caagtgaggt aatgataagg gattttattg aatactcgaa ttttaaaggg
     9241 tgtgactacg accttaaaga cttatctgaa gaatattact atttttatat tttggtgaat
     9301 ttagtttgga aaagaaaaga atataaagta gaaattcctc aaacagaatc ctttaatcaa
     9361 ctaaaaaaat ctattctcta catggagtta tccaaagcag ctcaaaagag tattgctata
     9421 catcttggta tagagttgaa tgaaggttta cttgattatc tatatttagt gttttgtatt
     9481 agtgatggtc ctctatttag caatcgagat gatatcaata tcaacgaaga atttattttt
     9541 gcttcttata agatatcaaa gtctaaagcc tttaagaatt tttgtggtcg attattgggt
     9601 agagaattta ttgactcgca gcattttaaa gatattaatg cctatttttt gagacatttt
     9661 atttgttatt gttattattt tattcctgat ttttattttt taaatacatc aaggctgagc
     9721 tattctaagg atttatatca cctattagat aaaggtctgg ctgatatttt taaccttaaa
     9781 ggagggaatt taactttttc aaaacatgag acagtgctac taacaatgca attatctaac
     9841 ttaatagaga ctttcttagc tcctctttct gtatatgtaa tttcatctag taatattaga
     9901 ttacaaacct atcaagtaat gttaaaccaa tattttacat ctaagatagc ggaattcttt
     9961 tttgtaaact atcaaacgac tcagatagat gaaaaattac taaaaaaagc agatatcatt
    10021 attgctgaga gacgctatat atcttcttta aaaaatgact caggtgtggc tatacaagat
    10081 atattagaaa ttgggatgaa taataagcag taccatgaac tacttttaca agctattatc
    10141 gaaaaaaggg accaaaaagt ttttgaatat atttcaaaaa tgaaacataa aatactttga
    10201 tagactctat atgagcttta ttaaaaggga taaaatcgaa atttaaaatt taattcgaaa
    10261 agatattgac agtaatctag aatttcgata tactagattt tgtcttttgg ggtatagcca
    10321 agcggtaagg caagggactt tgactccctc atgcgttggt tcgaatccag ctaccccagt
    10381 ttcacatggt ccgttggtca aggggttaag acaccgcctt ttcacggcgg taacacgggt
    10441 tcgaatcccg tacggactat cgtggccagt tgcattttag cggctggttt ttcttttatt
    10501 ttcaaaaata tcggagttac tatggaatac tttaacgttg gaaaaattgt caatacacaa
    10561 ggattacaag gcgaaatgcg tgtattaagc gtgactgact ttgtagaaga acgctttaaa
    10621 aaaggtcaag tgttagcttt attcgatgaa aagaatcagt ttgttatgga tattgaaatt
    10681 gctagtcatc gtaagcagaa gaattttgac attataaaat tcaaaggaat gtatcatatt
    10741 aatgatattg aaaaatataa aggtttcaca ctgaaggtgg cagaggatca actgagcgac
    10801 cttaaagatg gtgaatttta ttatcatgaa attattggct tagatgtcta tgaaggggaa
    10861 gagctgattg gaaaaatcaa agaaatttta caaccaggag caaatgacgt ttgggttgtg
    10921 gaacgtcatg gaaaacgtga tcttttatta ccctatatcc cgccagtagt attagaagtg
    10981 aacctttcta atcagcgtgt tcaagttgaa ttgatggaag gtttagatga tgaagattga
    11041 tattttaact ttatttcctg aaatgtttgc ccctctggaa cattctatag ttggtaaggc
    11101 aaaagaaaga ggacttcttg aaattaatta tcacaatttc agagaaaatg ctgagaagtc
    11161 tcgacatgtt gatgatgagc cttacggtgg ggggcaaggg atgcttttac gtgctcaacc
    11221 tatttttgat acaattgata aaatagatgc tcaaaaagca cgtgttattt tgctagaccc
    11281 agcgggcaga acatttgatc aggattttgc agaagaacta tccaaagaag atgaattaat
    11341 ttttatttgt ggtcactatg aaggttatga tgaacgtata aaatcattag taacagacga
    11401 agtatctttg ggagattttg ttcttacggg aggagaattg gcggctatga caatggtcga
    11461 tgctaccgtt cgtctgattc cagaagttat aggtaaagaa acaagtcatc aggatgattc
    11521 attttcatcg ggcctattag aatatcctca gtatacgcgc ccatatgatt atttaggcat
    11581 gacagttcct gatgttctca tgagtggtca tcatgaaaat atccgaaaat ggcgtttaga
    11641 acaaagtttg cgaaaaacac tagaacgtcg accagacttg ttagaaaatt atgcaatgac
    11701 ggatgaagag agacttatct tagagaaaat taaaacagaa atagaaagaa cagatacagt
    11761 aaatgaacaa aacaatttat gatatcacta ttgtaggagg cggaccagta ggtctctttg
    11821 ctgcttttta tgctggacta cgcggagtat ccgttaaaat tattgaaagc ctttcggaat
    11881 taggaggcca gccagctatc ctttatcctg agaaaaaaat ctatgatatt cctggttatc
    11941 cggtgattac tggacgtgaa ttaattgata aacatattga acaattagag agatttaaag
    12001 actctattga aatctgttta aaagaagaag ttttatcatt tgaaaaagta gatgatgttt
    12061 tcacaattca aacagataaa gatcaacatt tgtcacgtgc tattgttttt gcttgtggca
    12121 acggtgcttt tgctccgcgt ttactaggtt tggaaaatga agaaaattat gcagataata
    12181 atttattcta taacgttaca aagttagaac agtttgcagg taaacatgtt gttatttgtg
    12241 gtggaggcga ctcagctgtt gattgggcca atgaattaga taagattgca gctagtgtag
    12301 cgattgttca tcgtagagat gcttttagag cgcatgagca tagtgtggat atactaaagg
    12361 catcaggtgt tagaatttta accccttatg tcccaattgg tttaaatgga gatagtcaga
    12421 gagttagcag cttggtcgtt caaaaagtta aaggtgatga agtaattgaa ttacctttag
    12481 acaacttgat tgttagtttt ggcttttcta cttctaataa aaatttacgt tattggaatt
    12541 tggattataa acggtctagt atcaatgttt cttcactttt tgagacgact caagaaggcg
    12601 tgtatgctat tggagatgct gctaattatc caggaaaggt agaattgatt gcaacaggat
    12661 atggggaagc accagtcgct ataaaccaag ctatcaatta tatttatcct gatcgagata
    12721 accgtgtagt ccattcaaca tcattgataa aataactata aataaaactg ataagtttat
    12781 aaaaaaggat tatatatatt gttaaaaaaa taacaaaact tcctatttat tttgtgatat
    12841 aattaacttt gtcgttatga atgatattaa ataaatgaag cgaggtattt tatgacaaat
    12901 acggtaacac caaaagagac ggctggctct tttatcaata aagtgttagg tgggacagca
    12961 actgcaatcg ttgtagcact catcccaaat gctatcctag ccactttttt gaaaccattt
    13021 ttatcttacg gacttgctgc agagttctta cacatcgtgc aggtatttca atttttcaca
    13081 ccaattatgg caggattttt gattggtcaa cagtttaaat tcaccccaat gcaacagctt
    13141 gctgttggag gtgctgcata tattggttct ggtgcgtggg cttacacaga agttattcaa
    13201 aaaggagttg ctactggtag tttccaattg cgtgggattg gtgatctcat caatatgatg
    13261 ctaacagcag ctttagcagt tttagcagtt aagtggtttg gtaataaatt tggttcccta
    13321 accattatct tgttacctat tatcataggt acaggcgtag gctatttagg atggaaatta
    13381 ctcccttacg tttcatatgt gacaacactt attggtcaag gaattaattc ttttactact
    13441 ttacaaccaa ttgcgatgtc aattctaatt gcgatggctt tttcaatgct tattgtaagt
    13501 cctatttcaa cagtagcgat tggtctagcg attggtctta atggtatgtc agcaagtgca
    13561 gcttcaatgg gagttgcttc aacaacagca gtgctagttt gggcaacgat gaaggctaat
    13621 aagtctggag ttccaattgc tattgcttta ggtgcaatga agatgatgat gccaaatttc
    13681 ctaaagcatc cagtaatggc gattccaatg cttatgactg ctacagtaag ttctctaaca
    13741 gtcccattgt ttaaattggt ggggacacca gcttcgtctg gttttgggct tgttggtgca
    13801 gtaggtccga ttgcttcatt tgaagcagga gcaagtatgt tgattgttat cttatcttgg
    13861 ctcgttattc cttttgcagt aggttttgta tcacacaaaa tttgtaaaga cattctgaaa
    13921 ctttataaag acgatatttt cgtttttgaa ggtcaaaatt aatttggagg ttgtatgtta
    13981 gtttatattg ctggttcagg cgctatggga tgtcgttttg gatatcaaat ttctaaaaca
    14041 aatcatgatg ttattttact tgataattgg gcagaccata ttatggctat taaagaaaat
    14101 ggcttgaagg taacaggtga tacagaggac cttgttaaac ttcctatcat gaagccaact
    14161 gatgcgacag aagaagcaga tttaattatt ttgtttacta aagcaatgca gttgccaaat
    14221 atgcttcaag atattaaaaa aattattggt aagaaaacaa aggtcttatg tcttttaaat
    14281 ggattaggac atgaagatgt cattcgtcaa tatatccctg aacacaatat tttgatggga
    14341 gtaacagttt ggactgcagg gttgaaagga ccagggcatg ctcatctgga aggagttggt
    14401 tctgtaaatc ttcaaagtat cgatcctaac aatcaagaag caggtcatcg cgttactgaa
    14461 ttgcttaatg aagcaaaatt acaggctact tatgacgaaa atgttttgcc aaatatttgg
    14521 agaaaagctt gtgttaacgg aacgatgaat tcaacttgcg ccttgctaga ttgtacaatt
    14581 ggtcaactct ttgctagtga agacggcgta aatatggtac atgaaattat ccatgaattt
    14641 gttactgttg gaaaagcaga aggtgtagag cttgatgaag aagaaataac aaagtacgtt
    14701 atggatacat ctgttaaagc agcccatcat tacccttcaa tgcatcaaga cttagttcaa
    14761 aatcagcgct taactgaaat tgatttctta aatggcgcag tgaataaaaa aggtgaaaac
    14821 ctaggcattg atacacctta ctgtcgatta atcacacaat taattcacac taaagaaaat
    14881 gtacttagca taaaataata atcaaccggg agaatagtgc ccggtttttt gatatgctta
    14941 atgataatga ttttgttata ctaagaataa taagagttgg gtattttttt cgagaagtat
    15001 ttacagtata aaatattaca tttgtattac aaatatttct aaaatatgtt gacatgttac
    15061 aaaaaggata ttataatagt agtgtaaata aataaggaag taggtgttta tgatgaaaaa
    15121 aggacaagta aatgatacta agcaatctta ctctctacgt aaatataaat ttggtttagc
    15181 atcagtaatt ttagggtcat tcataatggt cacaagtcct gtttttgcgg atcaaactac
    15241 atcggttcaa gttaataatc agacaggcac tagtgtggat gctaataatt cttccaatga
    15301 gacaagtgcg tcaagtgtga ttacttccaa taatgatagt gttcaagcgt ctgataaagt
    15361 tgtaaatagt caaaatacgg caacaaagga cattactact cctttagtag agacaaagcc
    15421 aatggtggaa aaaacattac ctgaacaagg gaattatgtt tatagcaaag aaaccgaggt
    15481 gaaaaataca ccttcaaaat cagccccagt agctttctat gcaaagaaag gtgataaagt
    15541 tttctatgac caagtattta ataaagataa tgtgaaatgg atttcatata agtcttttgg
    15601 tggcgtacgt cgatacgcag ctattgagtc actagatcca tcaggaggtt cagagactaa
    15661 agcacctact cctgtaacaa attcaggaag caataatcaa gagaaaatag caacgcaagg
    15721 aaattataca ttttcacata aagtagaagt aaaaaatgaa gctaaggtag cgagtccaac
    15781 tcaatttaca ttggacaaag gagacagaat tttttacgac caaatactaa ctattgaagg
    15841 aaatcagtgg ttatcttata aatcattcaa tggtgttcgt cgttttgttt tgctaggtaa
    15901 agcatcttca gtagaaaaaa ctgaagataa agaaaaagtg tctcctcaac cacaagcccg
    15961 tattactaaa actggtagac tgactatttc taacgaaaca actacaggtt ttgatatttt
    16021 aattacgaat attaaagatg ataacggtat cgctgctgtt aaggtaccgg tttggactga
    16081 acaaggaggg caagatgata ttaaatggta tacagctgta actactgggg atggcaacta
    16141 caaagtagct gtatcatttg ctgaccataa gaatgagaag ggtctttata atattcattt
    16201 atactaccaa gaagctagtg ggacacttgt aggtgtaaca ggaactaaag tgacagtagc
    16261 tggaactaat tcttctcaag aacctattga aaatggttta ccaaagactg gtgtttataa
    16321 tattatcgga agtactgaag taaaaaatga agctaaaata tcaagtcaga cccaatttac
    16381 tttagaaaaa ggtgacaaaa taaattatga tcaagtattg acagcagatg gttaccagtg
    16441 gatttcttac aaatcttata gtggtgttcg tcgctatatt cctgtgaaaa agctaactac
    16501 aagtagtgaa aaagcgaaag atgaggcgac taaaccgact agttatccca acttacctaa
    16561 aacaggtacc tatacattta ctaaaactgt agatgtgaaa agtcaaccta aagtatcaag
    16621 tccagtggaa tttaattttc aaaagggtga aaaaatacat tatgatcaag tgttagtagt
    16681 agatggtcat cagtggattt catacaagag ttattccggt attcgtcgct atattgaaat
    16741 ttaaagctaa atctttttag atttagcttt actaatatta tgtgtgttgg aaaaaacagt
    16801 ggagatttac gtcaaaatta tattatttgg atagtatcga atagatagaa tgtggagaaa
    16861 agtaggaaat agttaagaaa ggtatttatg acgcttcgag aattaacaat agaagaattt
    16921 aaagaacatt caggaaatta tgattcacaa tcatttttac aaacacctga gatggctaaa
    16981 cttttagaaa aacgcggcta tgatgttagg tatttgggat atcaagtaga aaataaacta
    17041 gagataatca gtttatctta tattatgcca gtcactggtg gttttcaaat gaaaattgat
    17101 tcaggaccag ttcattcaaa ttctaagtat ctaaaacaat tttataaagc attgcaaggc
    17161 tatgccaaat ccaacggtgt tctagaatta atagttgagc cttttgatga ttaccaatta
    17221 ttcactagtt cgggagttcc tagtaatcag ggaaatgata atctgattga agattttacc
    17281 agttcaggtt atcaccatga tggtttaaca actggtttta ctggtaaata tttatcttgg
    17341 cactatgtta aaaatttaga aggtgtcact tctgaaacgt tactatcttc attctctaag
    17401 acaggacgag ctttggttaa gaaagcaatg tcttttggaa tcaaggttcg cgttcttaaa
    17461 cgtgatgagc tacatttatt taaagagata acaacttcta cgtcaaatag acgtgattat
    17521 atggataagt ccttagatta ttatcaagat ttttacgata gctttgaagg caaggctgaa
    17581 tttgtgattg ccactttaaa ttttagagaa tacgaccata acttgcaaat aaaagctgaa
    17641 gcattggaaa ataagcttaa attgcttgat gaacgtttta gggaaaatgc agactctcct
    17701 aagtatcata ggcaacgctc tgaaataatt aatcaactgg ctagttttga aacacgacga
    17761 caagaagttc agtcatttat tcaaaagtat gataaccaag acgttgtttt agctggtagt
    17821 ctatttgttt atagtcttaa ggagacagtt tatttctttt caggatccta cacagaattt
    17881 aataaatttt atgcaccagc agttcttcag gaatatgtta tgcaagaggc tctcaaaaga
    17941 ggttctactt tctataacct cctaggtatt tagggaactt ttgatggttc agatagtatt
    18001 cttcgcttta aacagaattt taatggttat atcattcgta aaatgggaac ttttaactat
    18061 tatccaagtc cttttaaata caaaggaatt cagcttctta aaaaggtttt gaagagaaat
    18121 taaagtattt gtaaaagcta atctttagat taggcttttt tagtttattg atagtttttg
    18181 gaagaaaatg cttgacattg aaagaaaaag agatataatt aaattgcaaa cgatttcaat
    18241 tcaaggaggg actatgctaa agtcaaagag aaaagaaatt atcttatcac gacttgagca
    18301 aaataagagt gttactttag atgaattaac gtctatctta gagacttctg aatctacagt
    18361 tcgcagggat ttagatgaac ttgaatctga aggtttttta aaaagggtgc atggtggtgc
    18421 tgaattacca tactcacttg gccaggaatt aagtaatcaa gaaaaatcta tcaaaaacgt
    18481 tcaaaaaaaa ttggatattg ctagacaaac tgcaaagctg atagcaaagc aagatgttat
    18541 atttattgat gccggaacta cgacagaatt attaattgat tttctccctc acgagcaatt
    18601 aacggtggtg acgaattcta ttcatcatgc tgcaaagtta gttgatagag ggataaaaac
    18661 aatcattatc ggaggagcgg ttaaacattc aacagatgct agtattggcc aggtagccat
    18721 taatcagatt aggcagataa cggttgacaa agcttttctg gggatgaatg gtatagatga
    18781 agtttattta actacaccag atttagaaga agctgctatt aaagaagcta ttattaataa
    18841 tagtcaacaa acctttattc taatggattc atctaaaatt ggacaagtta catttgctaa
    18901 agtgaaagag ataaatgaca ttaatttagt aactaataag actgattcag aacttatgac
    18961 aataataaaa gagaaaatga aggtgattca agtatgattt atacagtaac attaaatcca
    19021 tcgattgatt ttattgttcg tctagataca ctattgttgg gtagtgttaa ccgtatgact
    19081 agcgatgata aatatgtagg tggtaaagga attaatgtta gtcgcatatt aaaacgctta
    19141 aaaattgaca atactgctac aggttttatc ggtggattta caggacattt tgttgaagat
    19201 ggacttgtat cagaaggtat caaaactgac tttgtttctg ttaatgaaga tacacgtatc
    19261 aacgttaaag taaaagcaaa aattgaaact gaaatcaatg gcgggggtcc taggataaca
    19321 aatgaacaac ttcatcgtct agagaaactt ctttcgagac taacacctga ggacacagta
    19381 gttttcgcag gttcagcacc agctagcctt ggaaataagg tctataatac attgattcca
    19441 attgctaaaa agacaggagc ggaagttgtt tgcgattttg aaggtcagac acttttagac
    19501 gctcttgctt atcagcctct tttagtaaaa ccgaataacc atgaattagc agatattttt
    19561 ggcgtagagc tagaaggtct gcctgatatc gaaaaatatg cacataagat tctagataaa
    19621 ggagctaaga atgttatcgt ttctatggca ggggatggtg cattgttagt aactccagaa
    19681 gcttcatatt ttgcaaaacc gattaagggt gaagtgaaaa attccgttgg ggctggtgat
    19741 tcaatggtag ctggctttac aggagaattt gttaagtcaa aaaatccagt agaagcacta
    19801 aaatggggcg tagcttgtgg tacagcaacc actttctcag atgatttagc aacagctgag
    19861 tttatccaag atatttataa taaagttgag gtagaaaaat tatgaaaatc caagacctac
    19921 taaaaaaaga agttatgatt atggatttaa aagcaaccag taaagaggct gcaattgatg
    19981 aaatgattac aaaattagtt gacacaggtg ttgtgacaaa ctttgctatt tttaaagatg
    20041 gtatcatgaa gcgtgaggca caaacatcaa ctggattagg tgatggtatc gctatgccac
    20101 atagtaaaaa tgctgccgtt aaagaagcta ctgtcctttt tgcaaaatct gcatcaggtg
    20161 ttgattacga agcacttgat ggacagccaa ctgatttatt ctttatgatt gctgcgccag
    20221 atggtgctaa tgatacgcat ttagctgcct tggcagaact atctaaatat cttttaaaag
    20281 aagggttcgc tgatcaatta cgtcaagcaa aaacgccaga tgacattatt gctacttttg
    20341 atagtaatag catttctcaa gaaacagtag ctccacaaac agtacaatca acaagtaaag
    20401 gttcagatta tattgttgca gtaacagctt gcactacggg aattgcccac acgtatatgg
    20461 cagaagaagc tttaaagaaa aaagcagctg aaatgggtgt aggtattaaa gttgaaacaa
    20521 atggcgcttc tggtgtagga aataagttga cttcaagtga tattgcaagg gctaagggtg
    20581 ttattattgc agcagataag gctgtagaga tggatcgttt tgatggcaaa cctttggtat
    20641 cacgtccggt agctgatggt attaaaaaaa gtgaagactt aattaacatt attctcgata
    20701 ataaggcaca aacctatcat gcaaaaaatc aaaatgataa acaatcaggt gaatctgatg
    20761 gaaaatcagg cttaggctct gctttttata aacaccttat gggtggtgta tcacaaatgt
    20821 taccattcgt tattggtggt ggtattatga ttgcgattgc tttccttttt gataacatac
    20881 ttggtgtacc aaaagaccaa ttaagtaatt taggttcata ccatgaaatt gcagccttat
    20941 tcaaaaatat cggtggcgca gcatttgcat ttatgttacc agtattggca ggttatatcg
    21001 cttattcaat agctgaaaaa ccaggactag tagctggctt cgtagctggt tcaattgcat
    21061 caagtggact ggcattcggt aaagtacctt ttgcagaagg tgggaaagca accttagcat
    21121 tagcaggtgt gccatcaggt ttcttagggg ctttagtagg tggtttctta gctggtggag
    21181 ttatcctact acttagaaaa ttactttcag gtctcccaaa atcgcttgaa ggtattaaat
    21241 caattcttct atacccattg ttaggtgttt tgattacagg tttcttgatg ttgctcgtaa
    21301 acattccaat ggcagctatc aacacagctc ttaacacttt cttacaaggt ttatctggta
    21361 gttcagcagt cttgatgggc ttactagttg gtggtatgat ggctgtagat atgggtgggc
    21421 ctgttaataa agcagcttat gtttttggta caggtactct tgcagcaact gttgcaaatg
    21481 gcggctctgt tgttatggca gctgtaatgg ctggaggaat ggttccaccg ttagcagtat
    21541 ttgtggcaac tttattattt aaagataaat ttaataatga agaacgtcaa tctggtctaa
    21601 caaatatcgt tatggggtta tcttttatca ctgaaggtgc tattccgttt ggtgctgctg
    21661 acccagctcg tgcaatccct agctttattg taggttcagc cttaactgga gcacttgttg
    21721 gtttagctgg tatcaagttg atggctcctc atggagggat ttttgtcatc gcattaacaa
    21781 gtaatccttt actttatatt ctatttattc taattggagc gatagtctca ggtgtactct
    21841 ttggcttgtt ccgtaaaaca aaatagttaa tctcaactta tccaaattta cattgaataa
    21901 aaggtgaata aactatcgtt attgggtcta ttcg
//
LOCUS       DATROD010000020        21265 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.020, whole genome shotgun sequence.
ACCESSION   DATROD010000020 DATROD010000000
VERSION     DATROD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21265
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.020"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(1..109)
                     /locus_tag="VBC74_000782"
                     /pseudo
     CDS             complement(1..109)
                     /locus_tag="VBC74_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484319.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
     gene            complement(104..761)
                     /locus_tag="VBC74_000783"
                     /pseudo
     CDS             complement(104..761)
                     /locus_tag="VBC74_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018433.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            1072..1326
                     /locus_tag="VBC74_000784"
     CDS             1072..1326
                     /locus_tag="VBC74_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEO6941750.1"
                     /translation="MSYEQEFLKDFEEWLQSQISINQMAMDSAKKVLEEDKDERAADA
                     YIRYESKLDAYRFLQGKFNNYHNQKSFHDLPDGLFGQRHY"
     gene            1435..1746
                     /locus_tag="VBC74_000785"
     CDS             1435..1746
                     /locus_tag="VBC74_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941751.1"
                     /translation="MAKRIKPNKKRELLKAKGINWKDVRLSQTLQEVASPKKKAPEAP
                     KAVDLLEGLTANKQDVLKEAGLVSLEAFAKVSEADVLALKGIGPAAIKQLVDNGAVFA
                     K"
     gene            2014..2583
                     /locus_tag="VBC74_000786"
     CDS             2014..2583
                     /locus_tag="VBC74_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6941752.1"
                     /translation="MQILEDFDGKALPKLETDRLILRQRTVGDVPAMFDYVCLEEVAY
                     PAGLSPIASLEDEYDYFENRYYQNLEKAKLPSGYGITVKGSDRIIGSCDFNHRHEDDV
                     FEIGYMLHPDYWGHGYMTEAVAALIEVGFTLLNLHKIEIRCYDYNKQSRRVAEKLGFT
                     LEATIRDRKDNQGNRCVNLIYGLLRSEWE"
     gene            2681..3265
                     /locus_tag="VBC74_000787"
     CDS             2681..3265
                     /locus_tag="VBC74_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6941753.1"
                     /translation="MIKILGEIVDNQLPVVETNRLLLRQRKLEDAKEIFEFVKLDEVT
                     YPAGFPAVKSLEEEITYIQEIYPTNLEKEKLPSGYAITLKGDDKVIGSVDFNHRHEDD
                     IFEIGYLLHPDYWGQGIVPEAASVLVEIGFTLLGLHKIELGCYDYNKQSQAVARKLGF
                     TLEANIRDRRDAQGQRCGDMRFGLLRSEWEKKRR"
     gene            3262..3828
                     /locus_tag="VBC74_000788"
     CDS             3262..3828
                     /locus_tag="VBC74_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6941754.1"
                     /translation="MNIIIIGAQASGKMTIGQEIAKQTGMTLFLNHDSIDFVLRFMPW
                     SPDSIALTESIRFKFFETFAKTGQEMIFTIVIDFNDSRDVAFLEKIQTVFQSHNQEVL
                     FVELETELSERLKRNRTENHLKHKPSKRDIKWSENDICSTMDYAIFNPEVAPEALTYY
                     YKINNTCLTATETAYLIIQKINQIKEKN"
     gene            3828..6479
                     /locus_tag="VBC74_000789"
     CDS             3828..6479
                     /locus_tag="VBC74_000789"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEO6941755.1"
                     /translation="MSKELSPKYNPAEVEAGRYQTWLDQDVFKPSGDAEAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQG
                     ISRYDLGREKFLDKVWEWKDEYAATIKSQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYNKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGQNVILPIINKPIPIVADEHADPEFGTGVVKIT
                     PAHDPNDFAVGQRHNLPQVNVMNDDGTMNELADEFNGMDRFEARKAVVAKLESLGNLV
                     KIEKMTHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FMSWMENVHDWVISRQLWWGHQIPAWYNVNGEMYVGEDAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPDTEAADFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFSNVL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDQARENVEKVVNSQVGNVTDRWILHNLNETVGKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDEKVVTRSVLLYTLDQI
                     LRLLHPIMPFVTEEIFGQYADSSIVTAAYPTVNPAFENAAAHKGVESLKDLIRSVRNS
                     RAEVNVAPSKPITILVKTSDSELESFFKDNSNYIKRFTNPETLEISSAIATPELAMSS
                     VITGAEIFLPLADLLNVEEELARLEKELAKWQKELDMVGKKLGNERFLANAKPEVVQK
                     EKDKQADYQAKYDATQERIVEMQKLVK"
     gene            complement(6697..7476)
                     /locus_tag="VBC74_000790"
     CDS             complement(6697..7476)
                     /locus_tag="VBC74_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941756.1"
                     /translation="MLNSDFIISKSLANYIHHRRLEVGVSSTDLAEISNMSKSDWESF
                     EKNGGAIPLKSKDIILDLLFLERFPKEKECDFIDKLFEEAKENKLWPEKIYQTMGLTP
                     ALSFIAGCEILSDDINNDLEELSKLPKESHLGQLDTSLLLSLLPQQFITKYDYEFVYK
                     LSKVLAQYTSRNKVGSSYTAHSVIEEIYLYLIAKESILYFESLDENSHLQLKELLDYN
                     DEWPFDIFDDMDSYTFLYTDIYIEEDSPYHFKNWFVPQFYL"
     gene            8490..9908
                     /locus_tag="VBC74_000791"
     CDS             8490..9908
                     /locus_tag="VBC74_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HEO6941757.1"
                     /translation="MMTKETLAHYQKKIEQESEKKQSLDEHSWHVACFSRQEASIIGQ
                     GDVLFLIGLYHDLGKADRAFQDKLLNNPNRHVDHSYAGAKYLCSIIGPHLKSRGVDKG
                     ERMTFNEMVGYVISAHHGMYDFCYCSDDAEYYSFNKFKNRINRDLDDYHYHEDIKGYA
                     IKLEEKLCDYGYKDLRELIDKAFDNYQQAMSSLNWQDNSEWDYYQSCMVRLYLSLLKN
                     ADILDTVNAYGLKISPMDKTERSSLKHSYLAAIEQKYASFGRPNNQLNTIRTEIAERV
                     KERGKRDSKGIYRLDLPTGAGKTNLSMRYAFHQLVHQDKSRFFYITPFLSVLEQNASE
                     IRKVTGDLGVLEHHSNMVKQANEDDDKDSLLSAYLIDSWDSQVVLTSMVQFFQTLFKT
                     KSANLRRFSSLINSVVILDEVQSLPIEVTTLFNLTMNFFNKVMDTTIVLCTATQPAYD
                     SSEIDHRICYGGNLGELAEIVD"
     gene            10179..11138
                     /locus_tag="VBC74_000792"
     CDS             10179..11138
                     /locus_tag="VBC74_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6941758.1"
                     /translation="MLKVRYGVVSTAKVAPRFIEGVRLAGNGEVVAVSSRTLESAQAF
                     ANKYHLPKAYDKLEDMLADESIDVIYVATINQDHYKVAKAALLAGKHVLVEKPFTLTY
                     DQANELFTLAESCNLFLMEAQKSVFIPMTQVIKKLLASGEIGEVISISSTTAYPNIDH
                     VTWFRELELGGGTVHFMAPYALSYLQYLFDATITHASGTATFPKGQSDSQSKLLLQLS
                     NGVLVDIFLTTRLNLPHEMIIYGTEGRLIIPHFWKTTHAKLVRNDTSARTIQVDMVSD
                     FEKEAYHVSQMILEGQRVSHIMTPQLTLSGVKIIEGLYRSWGK"
     gene            11299..12201
                     /locus_tag="VBC74_000793"
     CDS             11299..12201
                     /locus_tag="VBC74_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO6941759.1"
                     /translation="MFSSDFGFEIINVDDLSLQEEKFLPSELLAYARDENESSFVRDI
                     EGHLTLVYQLLDTQGHVDDVRHVPRVIPVTLFLKEDGLFVLANHKNIILVKKALNRVE
                     KVDSPKHLLLSLVTAFSKQYFDVLDTISEERDKLINDLRKRPNKSNLARLANLQSGTV
                     HLMMGTKQNFEMLTDLQNIEQDKENTRNEKMQLQDAIIEARQLSNMCSLNSQVFQELS
                     SSYNNVLSNNLNDNVTTLTIISIGISIIAMVTSFYGMNVKLPFDSVDAVWVLIILITT
                     IITIMLSIVMYIYVHKEDNHPKHK"
     gene            12361..13425
                     /locus_tag="VBC74_000794"
     CDS             12361..13425
                     /locus_tag="VBC74_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEO6941760.1"
                     /translation="MSIESDFFRKKRFIFSSLEEFGFIKSDQEYIYCQTFMDNDFKAI
                     ITISLDGKIAGKVIDSALEEEYLPLRAANYNGSFVGEVRSAYMAILGDISDSCCKDLL
                     FTKDQSNRLAEKIATTFEDSVDYPFAKHPQYASYRVSGKWYALLFPLKMGKLENVPAQ
                     LSEEEVEVLNIKVNPQDMEILLQKEGIYPSYHMSKKTWVSIVLDNTLSDIEIFKLVSD
                     SRKLVSPNKKSNSEPEFWIIPANPKFYDIDKEFAEHQLIDWTQKGKIAVGDYVFIYIT
                     APIRALRYVCQVIERDLKTSTYLGARDVKAYMRLKLIRQFEDSTYPVAFLAEHGVKTV
                     RGPRRMTKELRQVMAKTILE"
     gene            13540..14532
                     /locus_tag="VBC74_000795"
     CDS             13540..14532
                     /locus_tag="VBC74_000795"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEO6941761.1"
                     /translation="MKKSFIHQQQEISFVKNTFTQYLIDKLEIVEVQGPILSQVGDGM
                     QDNLSGIEHPVSVKVLNIPEAEFEVVHSLAKWKRHTLARFGFNEGEGLFVHMKALRPD
                     EDSLDPTHSVYVDQWDWEKVIPDGRRNLDYLKETVEKIYKAIRLTELAVEARFDIESI
                     LPKRITFIHTEELVEKYPDLSPKERENAIAKEYGAVFLIGIGGELADGKPHDGRAPDY
                     DDWTTPSENGFKGLNGDILVWNEQLGTAFELSSMGIRVDEDALKRQVVLTGDEDRLEF
                     EWHKTLLRGFFPLTIGGGIGQSRLAMFLLRKKHIGEVQSSVWPKEVKDTFENIL"
     gene            complement(14577..15026)
                     /locus_tag="VBC74_000796"
     CDS             complement(14577..15026)
                     /locus_tag="VBC74_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001893380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin transport accessory protein"
                     /protein_id="HEO6941762.1"
                     /translation="MIKKLLLTLIAILCLTTLKSNLSSADTMTISQQQYQHMITNSDL
                     IAITDKALVSKSQQSEATIFIGRPTCQYCRAFLPKLLKSQATLHSKIYYLDSQKYKGK
                     RLKSFFKKHHITTVPNLAHYQRGKMTKYLVQGSQATPQQIQTFLSQK"
     gene            14992..15696
                     /gene="rsmD"
                     /locus_tag="VBC74_000797"
     CDS             14992..15696
                     /gene="rsmD"
                     /locus_tag="VBC74_000797"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEO6941763.1"
                     /translation="MAIKVNNNFLIIMGTSFLLLLAYHIKKEGKEILSSLFVISNFFD
                     NSTLFFVIIVVMRVVAGTFGGRPLKTLDGKTTRPTTDKVKGAIFNMIGPFFEGGRVLD
                     LFSGSGSLAIEAISRGMDQAVLVEKDRRAQVVIQENIAMTKSPEQFQLLKIEANRALE
                     QLTGQFDLVLLDPPYAKEEIVKQIQIMDSKGLLGDDIMIVCETDKSVDLPEEIASFGI
                     WKQKIYGISKVTVYVR"
     gene            15708..15998
                     /locus_tag="VBC74_000798"
     CDS             15708..15998
                     /locus_tag="VBC74_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941764.1"
                     /translation="MDYKLVMIILILVILIYLLLRLHYLEELLKHYHFKNDEGVCSLS
                     RTKNPLYLLMAMITFLPNLINWIANGFEAIDIVRLIPVVVFLWLYYCGDDDE"
     gene            15995..16480
                     /gene="coaD"
                     /locus_tag="VBC74_000799"
     CDS             15995..16480
                     /gene="coaD"
                     /locus_tag="VBC74_000799"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEO6941765.1"
                     /translation="MTKKALFTGSFDPVTNGHLDIIERASYLFDHVYIGLFHNLEKQG
                     YFSIECRKKMLEEAIRQFKNVSVLVAQDRLAVDLAREVGAKYFVRGLRNSQDFDYEAN
                     LEFFNKQLADGIETVYLSTSPSLSPISSSRIRELIHFKASVKPFVPKSVFREVEKMSE
                     E"
     gene            16470..17543
                     /locus_tag="VBC74_000800"
     CDS             16470..17543
                     /locus_tag="VBC74_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEO6941766.1"
                     /translation="MKNKDPKRKHKSLLGLLKWWIIGFAFLLLVLASLVVRLPYYLEM
                     PGGAYDIRSVLKVNKKADKAKGSYNFVAVSVSQATPAQVLYAWLTPFTELSSKEETTG
                     GFSNDDYLRINQFYMETSQNESIYQALKLANKQVSLTYKGVYVLNLAKNSTFKDRLHL
                     ADTVTGVNGKSFKNSSQLIKYVAALHLGDKVKVQYTSQGKKKESVGKVIKLSNGKNGI
                     GIGLTDHTEVSSDVPVDFNTEGVGGPSAGLMFTLAIYDQLVKEDLRKGRKIAGTGTIE
                     QNGHVGDIGGAGLKVVSAAKKGMDIFFVPNNPIDKNAKKGKTKVQTNYQEAKAAAKRL
                     GTKMKIVPVQNVQQAIDYLKKTK"
     gene            17618..18952
                     /locus_tag="VBC74_000801"
     CDS             17618..18952
                     /locus_tag="VBC74_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEO6941767.1"
                     /translation="MTELIRILHLNDLHSHFENFPKVKRFFHNNQAQPIETISLDLGD
                     NIDKSHPLTEASSGKANVQLMNELGIELATIGNNEGVGLSKKDLDQVYKDSDFTVIVG
                     NLKDNIIEPSWAKPYIIYETQQGTKLAFLAYTFPYYKTYEPNGWTIEDPIDCLKCHLQ
                     INEIKEANCRILMSHLGIRFDTRIAQEFSEIDLIIGAHTHHLFEEGELITGTYLAAAG
                     KYGRFVGSIDITFDNHTLKDILISTYDTKQLTGYPSDSDWAKEIESEGKEQLRKESLI
                     SFPAPLSLEESCQLVMDAMLFYAGADVAIINSGLIVQPFEKDFSRKNLHESLPHQMRL
                     AKLTVSSQELLEIYETIYQQGQFLAQQKIHGMGFRGKYFGEVLHSGFDYKNGKIVYNE
                     KDIDAKEEVILVIVDQYYFASYFECLKTKKVDLLFPELLRDVVADYLINMTL"
     gene            19021..19599
                     /locus_tag="VBC74_000802"
     CDS             19021..19599
                     /locus_tag="VBC74_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEO6941768.1"
                     /translation="MRKEVTPEMLNYNKYPGPQFIHFENIVKSDDIEFQLVINEKSAF
                     DVTVFGQRFSEILLKYDFIVGDWGNEQLRLRGFYKDASTIRKNSRISRLEDYIKEYCN
                     FGCAYFVLENPNPRDIKFDDERPHKRRKSRSKSQSSKSQTRNNRSQSNANAHFTSKKR
                     KDTKRRQERHIKEEQDKEMTSAKQHFVIRKKK"
     gene            19650..20756
                     /gene="rlmN"
                     /locus_tag="VBC74_000803"
     CDS             19650..20756
                     /gene="rlmN"
                     /locus_tag="VBC74_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEO6941769.1"
                     /translation="MPKKTDTPDYKPSIYSLTRDELIAWAIEHGEKKFRASQIWDWLY
                     KKRVQSFDEMTNISKDFIALLNENFVVNPLKQRIVQESADGTVKYLFELPDGMLIETV
                     LMRQYYGLSVCVTTQVGCNIGCTFCASGLIKKQRDLNNGEITAQIMLVQKYFDERGQG
                     ERVSHIVVMGIGEPFDNYTNVLKFLRTVNDDNGLAIGARHITVSTSGLAHKIREFANE
                     GVQVNLAVSLHAPNNELRSSIMRINRSFPLEKLFAAIEYYIETTNRRVTFEYIMLNGV
                     NDTPENAQELADLTKKIRKLSYVNLIPYNPVSEHDQYSRSPKERVEAFYDVLKKNGVN
                     CVVRQEHGTDIDAACGQLRSNTMKRDRQKAKVGR"
     gene            20756..>21265
                     /locus_tag="VBC74_000804"
     CDS             20756..>21265
                     /locus_tag="VBC74_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEO6941770.1"
                     /translation="MLKRLFTEDGELTKISRRFVWMLVVIYCLIIVRMCFGPQIMIEG
                     VSTPNVQRFGRIVALLVPFNSFRSLDQLTSFKEIFWVIGQNVVNILLLFPLIIGLLSL
                     KPSLRKYKSVILLAFLMSLFIECTQVVLDILIDANRVFEIDDLWTNTLGGPFALWSYR
                     NIKGWLLTIR"
BASE COUNT         6857 a         3380 c         4284 g         6744 t
ORIGIN      
        1 taataaggct atctttttca aggatatgtt tctgatcacc aaggataatt tcaagttttc
       61 tttccaaaac atttattaat tttggatgag ggcatcgttc atttttcagt ccccggctga
      121 tgattttaat ggtattgaat aaaccttcat ctttaatcat tcctaacggg gacataagac
      181 ttatttttcc aaattgatat tctgaacttt aaaaactatt ttgtaagaat aaattttgcc
      241 aatcatctat ttttagagga gcaacgttaa cattcatagt atgtctaagt tgatcgatca
      301 gcttggcagt gtcttcatct tgataagaaa catcatgtgt gagtaaacgt ccaccaggtt
      361 ttaggacacg taagtactct tttatggctt tctccttagc tgaattgtta agcatcgtta
      421 acattgcttc gttgataacg ataaactatt atcaggaaag ggcagtctga gtgcatttcc
      481 ctgaataact tcaataaatt cttctaattg ggcctttttg acattttctt tggccttttc
      541 tattacttta ctatctagat caagcgctgt aatgtgacag gaaaatgctt tagcgatatc
      601 gatggaactg gtacattgat tacaagctac ttctagaaca cgactgtcag cactaaagtg
      661 tccttttttg ataagccaat tcgttgcttg gacacctcct ggtcttaagc gttttttcct
      721 agtcgtgcta agaattgatg tccaatttct ttatgtggca ataagatcac ctctttcttt
      781 taatagtaat atcatttttt cttaattata acacatagat gctaagataa caaggagtaa
      841 tagtgattga atggtataag tgatgaattt tagagagcgt tttcttttaa ctaggggtgt
      901 gatgccaaaa ctagaatggc atagagaacc atacatctca aaaggtcttc cttagataaa
      961 actagttgtt tttatatctg tcagaaaaat cataatttat ttttgatata atgatttttt
     1021 gagtatttaa tttatatgtt ataatgaaaa aaactaaaaa agaggaatat catgtcatac
     1081 gaacaagaat ttttaaaaga ctttgaagag tggttacaat cgcaaataag tatcaaccaa
     1141 atggcaatgg acagtgcaaa gaaagtttta gaggaagata aggatgagcg agcagcagat
     1201 gcgtatattc gttatgagag taagttagac gcttatcgct ttttgcaagg gaaatttaat
     1261 aactatcata accaaaaatc tttccatgat ttgcctgatg gtctttttgg acaacgtcat
     1321 tattaaaagg tttatcgcta gaaagcttgt caaataggca tttctatgat acattgttat
     1381 agtagttttt agatactatg atagatttca taaatgagga gaaagcatta tacaatggca
     1441 aagcgtatta aaccgaataa aaaaagagag cttttgaaag ctaaagggat caattggaag
     1501 gatgttcgac tatctcaaac tttacaagaa gtcgcatcac cgaaaaaaaa ggctcctgaa
     1561 gcgccaaagg cagtcgatct tttagaaggt ttaactgcta ataaacaaga tgttttaaaa
     1621 gaagctggcc ttgtcagcct tgaagcgttt gcgaaagtaa gtgaagcaga tgttttagca
     1681 ctaaaaggta ttggtccagc agctattaaa cagttagttg ataatggtgc tgtttttgca
     1741 aaatgatttg attcaccttg cgaaagtagt cttattttgc tagaataaat taacttagag
     1801 gtgttttgaa atcccatttg tcacaggaag cggtcatact gagaaatccg cacaaatgtc
     1861 aaagcttgtt gctaagtcca ataattgaaa caaaacgagt tgcggatgtt atcatccgaa
     1921 tttgggtggt accgcggaaa tcgtaatacg aaattcgtcc ctgtcagaat tgacagggat
     1981 tttttgtatt taggagtgag gagattgaag tatatgcaga ttctagaaga tttcgatggc
     2041 aaagcattac caaaactaga aacggatcga cttatcttgc gtcagcgaac ggtgggggat
     2101 gtcccagcca tgtttgatta cgtttgcttg gaagaggttg cctatccagc agggctcagt
     2161 ccaatagctt ctttagaaga tgagtatgat tattttgaaa atcgttacta tcaaaatctg
     2221 gaaaaggcaa aattaccatc tggttatggt attaccgtta aaggaagtga ccggattatc
     2281 ggctcttgcg actttaacca ccgccatgaa gacgatgttt tcgagattgg ctacatgctt
     2341 catcctgact attggggtca tggttatatg acagaagctg tcgcagcctt gattgaagtc
     2401 ggttttaccc tcctcaatct ccacaaaatc gaaatccgct gctatgatta caataagcaa
     2461 agccggcgcg tggcagaaaa actaggcttc acccttgaag ccaccatccg tgatcgtaag
     2521 gataatcaag gtaatcgctg tgtcaatttg atatatggtt tgctgaggag tgagtgggag
     2581 taattattct actcttcata gaagttacta gatagactaa tttggctgtg cagggtactt
     2641 agctaactca tcaataatat cgtagatatg gaggagacaa atgattaaga tacttggaga
     2701 aatcgtagat aaccagctac ctgttgtgga aacgaatcgt ctgttgttgc gtcaacgcaa
     2761 gttagaagat gctaaggaga tatttgaatt tgtcaaactt gatgaggtga cttatccagc
     2821 aggttttccg gctgttaaaa gcttggaaga agagataacc tatattcaag agatttatcc
     2881 aactaatctt gaaaaagaaa agttaccctc tggttatgct attactctaa aaggcgatga
     2941 taaggtaatt ggttcagtag attttaacca tcgccacgaa gacgacattt ttgaaattgg
     3001 ctacctgctc caccctgatt attggggaca aggcattgtg ccagaagcag cttcagtcct
     3061 tgtggagata ggtttcacac tgctaggact acataagatt gagctgggat gctatgacta
     3121 taataagcaa agtcaggcgg ttgcgcgcaa attaggtttt actttagagg ctaatattcg
     3181 tgatcgaaga gatgcccagg ggcaacgttg tggcgatatg cgctttggtt tactgcgaag
     3241 cgagtgggag aaaaaaagaa gatgaatatt attatcatcg gagcacaagc ttctgggaaa
     3301 atgacaattg gtcaggaaat cgcaaaacag acaggcatga ccttattcct taaccatgat
     3361 tctatagatt ttgttttacg cttcatgcca tggtctccag atagtattgc tctaacggag
     3421 tctattcgct ttaaattttt tgaaacgttt gctaagactg gtcaagaaat gatttttact
     3481 attgtaattg atttcaatga ttcaagagat gtagcatttc tagaaaagat tcaaacagtt
     3541 tttcaatctc acaaccaaga ggtattattt gtagaattgg aaacagagct aagtgagcgt
     3601 ctcaaacgaa atcgcacaga aaatcacctc aagcataagc cttcaaaacg tgatatcaaa
     3661 tggtcagaga acgatatttg ttcaaccatg gattacgcta tctttaatcc tgaagtagcc
     3721 ccagaagcgc ttacatatta ttataaaatc aataatactt gcttaactgc tactgaaaca
     3781 gcatatctta ttattcaaaa aataaatcaa atcaaggaga aaaactaatg tcaaaagaac
     3841 tatcaccaaa atacaatcct gccgaggttg aggcaggccg ttatcaaact tggcttgacc
     3901 aagatgtttt caagccatca ggggatgctg aggctaagcc ttattctatt gtgattccac
     3961 caccaaacgt tacgggtaaa ttgcacttgg gtcacgcttg ggatacaact ctacaagata
     4021 tcattattcg tcaaaagcgt atgcagggct ttgatacact ctggcttcca ggtatggacc
     4081 acgctggtat agccactcaa gctaaagttg aggagcgcct tcgtgagcaa ggcatttcac
     4141 gttatgatct tggtcgtgaa aaattcctag ataaagtttg ggaatggaaa gacgagtatg
     4201 cagcaactat taaatctcag tgggggaaaa tgggtctttc ggttgattat agccgtgagc
     4261 gttttaccct tgatgaggga ctttcaaaag cggtaagaaa agtctttgtt gatttgtaca
     4321 ataaaggttg gatctatcgt ggggagttca tcattaactg ggatccagca gctcgtacag
     4381 ccctttcaga tattgaagtt attcacaagg atgttgaggg tgctttctat catatgaatt
     4441 atatgctgga agatggctca cgcgccctag aagtagcaac aactcgtcct gagactatgt
     4501 ttggagatgt ggctgtagcg gtaaatccag aagatccacg ttacaaggat ttgattggtc
     4561 aaaatgttat ccttcctatc atcaataaac ctattccaat cgtcgctgat gaacacgctg
     4621 atccagaatt tggaacaggt gtggttaaga ttacgccggc tcacgaccct aatgacttcg
     4681 ctgtaggtca acgccacaac ttaccacaag ttaacgttat gaacgatgat gggactatga
     4741 atgaacttgc ggatgaattt aatggtatgg atcgtttcga agctcgtaag gcagtagtcg
     4801 ctaaattaga atcgttagga aacttagtaa aaattgaaaa aatgactcat tctgttggtc
     4861 attcagaacg aacaggcgtt gtggttgaac cacgtttatc tactcaatgg ttcgtcaaga
     4921 tggatcaatt ggctaagaat gccattgcca accaagatac agaagacaag gtagagtttt
     4981 atccaccacg atttaacgat acttttatga gctggatgga aaatgtccat gactgggtta
     5041 tctctcgtca attatggtgg ggacaccaaa ttcctgcatg gtacaacgtc aatggtgaga
     5101 tgtatgttgg agaagatgcc ccagagggtg acggatggac acaggatgaa gacgttctag
     5161 acacatggtt tagttctgct ttatggccgt tttcaacaat gggatggcca gacaccgaag
     5221 cagctgactt caaacgctac ttcccaacgt caaccttggt aactggatac gatatcatct
     5281 tcttctgggt atcgcgcatg attttccaat ctcttgaatt tacaggacgc caaccattct
     5341 caaatgttct tattcatgga ctcatccgtg atgaagaagg acgtaaaatg tctaaatcac
     5401 ttggcaatgg gattgatccg atggatgtca ttgagaaata tggtgcggat gctcttcgtt
     5461 ggttcctctc aaatggttca gcccctggtc aagatgtgcg tttttcatac gaaaaaatgg
     5521 atgcttcgtg gaatttcatt aataagattt ggaacatttc acgctatatc cttatgaata
     5581 acgaaggttt aacgttggat caagccagag aaaatgtgga aaaagttgtt aatagtcaag
     5641 ttggaaatgt aacagaccgt tggattctcc acaatttaaa tgagactgtg ggtaaagtta
     5701 ctgaaaactt tgacaagttt gaatttggtg ttgctggtca catcctttac aactttatct
     5761 gggaagagtt tgctaattgg tacgttgagt taacaaaaga ggttctttat agtgacaatg
     5821 aagatgagaa agttgttaca cgttctgttc ttctttatac cttagatcaa atcttgcgtc
     5881 ttcttcatcc aatcatgcca ttcgtgacag aagaaatctt tggtcagtat gcagatagct
     5941 caatcgtaac agctgcttac cctacggtta acccagcctt tgaaaatgct gctgcccaca
     6001 agggagttga aagcttgaag gacttgattc gttccgtgcg taatagccga gcagaggtca
     6061 atgttgctcc atctaaacca atcactatct tggttaagac aagtgacagc gaactcgaaa
     6121 gcttcttcaa agacaatagt aactacatca aacgtttcac aaacccagaa acacttgaaa
     6181 tcagctcagc tattgccaca ccagaacttg ccatgtcatc agtcatcaca ggagctgaaa
     6241 tcttcctccc acttgctgac ctcctcaacg tagaagaaga gttagcacga cttgaaaaag
     6301 aactagccaa atggcagaaa gaactggaca tggtaggcaa aaaactcggc aacgaacgct
     6361 tcctcgccaa cgccaaacca gaagttgtcc aaaaagaaaa agacaaacaa gccgactacc
     6421 aagctaaata cgacgcaacc caagaacgga ttgtagagat gcaaaaactt gttaaatagt
     6481 taaaatcatc cattacttcc gcagagtcct gccgctagac catcagtcta agcgacaggg
     6541 ctttttgcta tattcaaaga ttgtgaaaaa ttaatatata tgatgcctac ttcttgcgtc
     6601 tgaaatacaa gaagtgaaaa tgatttggcc ataaagaaaa acaggcagaa acccaaatga
     6661 ttaatgccta tttcttatat tttttcgtca attattttat aaataaaatt gaggtacaaa
     6721 ccagttttta aaatgataag gtgaatcttc ttcaatatag atgtctgtat ataaaaaggt
     6781 atagctatcc atatcatcaa aaatatcaaa aggccactca tcgttataat ctaataattc
     6841 cttaagttgt agatgggagt tttcgtccag agattcaaaa taaagaatgg attcttttgc
     6901 aattaaatag agataaattt cctctattac actatgcgct gtataagatg aaccaacttt
     6961 atttcttgat gtatattgag ccaaaacttt gcttaattta taaacaaact cataatcata
     7021 ttttgtgata aattgttgag gcaatagtga taaaagtagg gaagtatcta attgtcccag
     7081 atgactttct ttaggtagtt tagataattc ctccaaatca ttattaatat catcagaaag
     7141 tatttcacaa ccagcaataa atgaaagagc aggagtaagt cccatagtct ggtaaatctt
     7201 ttcaggccaa agtttatttt cctttgcttc ctcaaataat ttatctataa aatcacattc
     7261 tttctcttta gggaatctct ccaaaaataa taagtcaagt atgatatcct tagatttcaa
     7321 aggaattgcc ccaccatttt tttcaaatga ttcccaatct gatttagaca tattggatat
     7381 ttctgccaaa tcagttgacg acacgccaac ttctaaccta cgatggtgaa tgtagttagc
     7441 aagtgactta ctaataataa aatcagaatt tagcattccg ttctcctttt gattcctatt
     7501 cattatgatt atcacatata aaaaattttt ttcaagtaaa aacacatgca tcttttgatt
     7561 tagcacatca tgattttcta gagatgttgt aatgattcat aatattagta gtattttcta
     7621 ttttctatca atttgggttt gtttagtagt tttaacattc cttaaaaact taattttata
     7681 acgagcaaac aaaaatatat gctaatctac ttctttttat gcgtaaagat atattttaaa
     7741 atgacaatca gattataaag gaacataatg ctaaaaatga atagacatag aaagtgaaga
     7801 tacatgagga ctttataaat agcaggaaag tagatactag ttagatatag tagtgcaaga
     7861 tagatgagaa aaaataacga atactttaac ttggtcataa caattccatt ctaatggtag
     7921 taagcatgca taaaatgtag gttttatcat actacttgaa tccagcaact agatagaggg
     7981 ggttacatac gataagacag tagtatttta aaccgatcgg ttgacttatt gatactgttt
     8041 agggaacaag tgttttgttg tcaatgttaa atttaattta gcattttgat ggtatcaccg
     8101 caagaatgaa aggaaaagtt gtatgtgctg aaaaaactaa tgcttggcga tttagaacac
     8161 acgtttacta gtcgagatga caaaaaataa gtaccaaatg taaaggtcga tattatcagc
     8221 attgtcagcg ttaggtgtta taaaaattta acatcttcca attaagctag gtttaagttt
     8281 cctaagttga actacgccta gccgaaaaat aatttctttt ttacagaact cctaacaagg
     8341 attttgaatt gtcaagattc ttgttttttt ggaaagtcat ttagaggctt tttgggggaa
     8401 gtattaacta ttgtatccgt ttacaattcc tttcaaaaat gttagtatat cattgaggga
     8461 tatgagttat ggataaggga gagaggatga tgatgactaa ggaaacgttg gcacattatc
     8521 aaaagaaaat agaacaagaa tctgaaaaaa agcagtcttt agatgagcac tcatggcatg
     8581 tggcctgttt tagtcgacag gaagcatcta taattggtca aggagatgtg ctttttttaa
     8641 ttggtctata ccacgatcta ggcaaagctg atcgagcctt tcaagataaa ttattaaata
     8701 atccaaatcg acatgttgat cattcttatg caggggcaaa atacttatgc tctattattg
     8761 ggcctcatct aaaaagtcga ggggttgata aaggtgagag aatgacattc aatgaaatgg
     8821 tgggttatgt catctcggct catcatggga tgtatgattt ttgctactgt tctgacgatg
     8881 ctgaatatta tagctttaat aaatttaaaa accgtatcaa cagagactta gatgattatc
     8941 actatcacga agatattaaa gggtatgcta taaaattaga agaaaaattg tgtgattacg
     9001 gctacaaaga tttaagggag cttattgata aagcttttga taattaccaa caagctatgt
     9061 cttccttaaa ctggcaagat aatagtgagt gggattatta tcagtcttgt atggtgagac
     9121 tttacttgtc gctcttaaaa aacgctgata ttttggacac agtaaatgcc tatggtctta
     9181 aaataagtcc tatggataaa acagagcgat cctctctaaa acattcttat ttagcagcca
     9241 ttgaacaaaa atatgctagc tttggacgac caaacaatca gttgaatact attcggacag
     9301 aaattgctga gcgtgttaaa gaaagaggta aacgggattc caaagggatc tatcgcttag
     9361 atttaccgac aggagctggc aagactaatc ttagtatgcg ctatgcgttt caccaattag
     9421 ttcatcaaga caaatcaaga tttttttaca taacgccctt tctttcggtt cttgagcaaa
     9481 atgcttccga aattagaaaa gttacgggtg accttggcgt tctagaacac cattccaata
     9541 tggttaaaca ggctaatgaa gatgatgaca aggacagttt attgtcagct tatcttattg
     9601 atagctggga tagtcaagta gtcttgactt ctatggttca atttttccaa acgcttttta
     9661 aaacaaaatc ggctaatttg agacgttttt caagtttgat taatagtgtt gtgattctag
     9721 atgaagttca gtcactgcct attgaagtca ccactttgtt taatttaacg atgaattttt
     9781 ttaataaagt tatggataca accatcgttc tttgtacagc aacacaacct gcttatgatt
     9841 cttcagagat tgaccatcgt atctgttatg gagggaattt gggagaatta gctgaaatag
     9901 tggattgaaa taagtttata cgttgcttca tcaccaagtt ttacgtcgca cccattgcgg
     9961 gtgcgtggat tgaaatataa gttcagcaac ttcacgagaa aatgttcctc gtcgcaccct
    10021 ttgcgggtgt gtagtttcaa ctattttaaa tctttcaaat tatgctatta aaattgtcta
    10081 acttttaaca gagatgcgat tgatttgtat taatccctaa aaagataaat tgctagttct
    10141 tatgctataa taactctatc aatgtcaaag gagtttttat gttaaaggta agatatggtg
    10201 ttgtgtcaac ggcaaaggtg gcgcctcgtt ttattgaagg agtccgctta gcaggaaatg
    10261 gtgaagttgt ggctgtatcg agtcgaactc ttgagtctgc gcaagctttt gccaacaaat
    10321 atcatctccc aaaggcttac gataagttag aggatatgtt agcagatgag tcgattgatg
    10381 tgatttatgt ggcaacaatt aatcaagacc attataaagt tgctaaagca gctctgttag
    10441 cgggaaaaca tgtgttggta gaaaagcctt tcacattgac ttacgaccaa gcaaatgaat
    10501 tattcacttt ggcagaaagt tgtaatctgt ttttgatgga agcacaaaaa tcggtattta
    10561 ttccgatgac acaagtgatt aaaaaattac ttgcctctgg tgaaatagga gaagtgatct
    10621 ccatctcatc tacaacagcc tacccaaaca ttgaccatgt aacatggttt agagaacttg
    10681 agttaggtgg aggaacagtg cattttatgg cgccctatgc cctgtcttat ttacaatacc
    10741 tatttgatgc gactataact catgctagtg ggactgcgac ttttccaaaa ggtcaaagcg
    10801 atagtcaatc caaactactc ttgcaattat caaatggggt cctcgtagat atcttcttga
    10861 caacacgttt aaatttaccg catgaaatga ttatttatgg aactgaaggt cgtctaatca
    10921 ttccacattt ttggaaaacc actcatgcaa aattggtgag aaatgatact agtgcgcgaa
    10981 cgattcaagt tgacatggtt agtgattttg agaaagaagc ttatcatgtt agtcaaatga
    11041 ttttagaagg gcaaagggta agtcatatca tgacgcctca gttgacgctg tcaggtgtta
    11101 agattataga aggcttatac cgttcatggg ggaaatgatg ctggctatct agcatattaa
    11161 taaggagtac tagatgttat tttagtagtc cttttttgtt ttgctctcat aaggttaatt
    11221 agatttaaaa gggttttcta cttgtttttt ctcgattttt gtttcataat gtagacaagt
    11281 ttagagtgag gtgcttctat gttttcgagc gattttggat ttgagataat taatgttgac
    11341 gatctatcct tacaagagga gaaattttta ccttcagaat tgctggctta tgcacgtgat
    11401 gaaaacgagt cttctttcgt acgtgatatt gaggggcatt tgacacttgt ttaccagctt
    11461 ttggatacgc aaggtcacgt tgatgatgtt cgtcatgtgc cgagagtgat accagtgact
    11521 ctatttttga aagaagatgg cttgtttgta ttagctaatc ataaaaatat tatccttgta
    11581 aaaaaagcac ttaatcgcgt tgaaaaggta gattcgccga aacacctctt attatcatta
    11641 gtgactgctt tctcaaagca atattttgat gttttagata ctatttctga agaaagagat
    11701 aaactaatca atgatttgcg taaaagacca aacaaatcga acttggcaag gctggcaaat
    11761 ttacaaagtg gaacagtaca cttaatgatg ggaactaagc aaaattttga aatgctgacc
    11821 gatttacaaa acattgagca agataaagag aatactagaa atgaaaagat gcaacttcaa
    11881 gatgctatta ttgaagcaag acaattatct aacatgtgtt ccttaaattc acaggttttc
    11941 caagaacttt cttcttctta taataatgtc ctcagcaata accttaacga caatgtaact
    12001 accttaacaa ttatttccat tggtatttcc attattgcta tggttaccag cttttatgga
    12061 atgaatgtta agttgccttt tgatagtgtt gatgctgtct gggtattgat tattttaatt
    12121 acgactatca ttactattat gctctctatt gttatgtaca tttatgtcca taaagaggat
    12181 aatcatccca aacataaata gagaattaat aagagggtgc tatcaattag tttagtttta
    12241 agcatggcaa catgcttttt ttgtacaaag aaactagaat tcatctttga ctttgagctt
    12301 gctttctaac tcttaaaacg acataataga ggataatgta cagtgaatag agaggttact
    12361 atgtcaatag aatcagattt tttccggaaa aaacgtttta ttttttcatc gcttgaggag
    12421 tttggattta tcaaatctga ccaagagtat atctattgtc aaacatttat ggataatgac
    12481 tttaaagcta ttatcacgat ttcattggat ggaaagatag ctggtaaggt tattgattcg
    12541 gcacttgaag aagaatattt accgcttcga gcagcaaatt ataatggttc gtttgttggc
    12601 gaagtacgat cggcttatat ggctatatta ggtgatattt cagattcttg ttgtaaggat
    12661 ttattattta ccaaagatca aagtaatcgt ttagcggaga agatagctac aacttttgaa
    12721 gattcagttg attatccatt tgcgaagcac ccacaatatg cttcttatcg tgtatcaggt
    12781 aaatggtatg ctcttctttt tccattgaag atggggaagc tagaaaatgt tccagctcaa
    12841 ctgtcagaag aagaggttga agttttgaat attaaagtca atcctcaaga tatggaaata
    12901 ctcttacaaa aagaaggtat ttatccttct tatcatatgt ctaaaaagac ttgggtatct
    12961 atcgttttag acaatacttt gtctgatata gaaattttta aattggtatc agatagtcgt
    13021 aagcttgttt ctcccaataa aaaatcaaat tctgagccag agttttggat tattcctgct
    13081 aatcctaaat tttacgatat tgataaggag tttgcagagc atcaattgat tgattggact
    13141 caaaaaggaa agattgcggt gggagattat gtgtttattt atattacagc acctattcgt
    13201 gctttgcgct atgtctgtca agtgatagaa cgtgacctta aaacaagtac ttacttagga
    13261 gcaagagatg tcaaagctta tatgcgactc aaactgataa ggcaatttga agattctacc
    13321 tatcctgtag catttttagc agaacacggc gttaagactg tgagaggacc acggcgaatg
    13381 accaaggaat tgcgacaagt catggcaaaa acaattctag agtaatgact ttcagaaaaa
    13441 aacttgtaaa aaagtcagaa taattctaca attcctcttc cattttgtta cttgaaggag
    13501 tataatagct ctaacatttt ataatttaag gaggaacata tgaagaaaag ctttattcat
    13561 cagcaacagg agatttcttt tgtcaaaaat acttttacac aatatttgat tgataagtta
    13621 gaaatcgtcg aagtgcaagg acctattctt agtcaggtcg gggatggtat gcaagataat
    13681 ttatctggta ttgaacatcc tgtatcggtg aaagtcctta atatacctga agctgaattt
    13741 gaagtcgttc actcattggc taaatggaaa cgtcatactt tggcaagatt tgggtttaat
    13801 gagggagaag gtcttttcgt tcatatgaaa gctcttcgtc cagatgaaga ttctcttgat
    13861 ccaacacact cagtttacgt agatcagtgg gactgggaga aggtcattcc tgatggtaga
    13921 cgtaatttgg actacctaaa agaaactgtg gaaaaaatct acaaagctat ccgcctaaca
    13981 gagcttgctg tggaagctcg ttttgatatt gaatccattt taccaaagcg tattaccttt
    14041 attcatacag aagaattggt agaaaaatac ccagacttaa gccctaaaga gcgtgaaaac
    14101 gccatcgcta aggagtatgg cgcagtcttt ttgattggta ttggtggaga gttagcagat
    14161 ggtaaaccac atgatggtcg tgcaccggac tatgatgact ggacaacacc atcagaaaat
    14221 ggttttaaag gtctcaatgg tgatatttta gtatggaacg aacagcttgg aactgccttt
    14281 gaactatcat caatgggaat tcgagttgat gaagatgctc ttaaacgtca agttgttctc
    14341 accggtgatg aagatcgttt agagtttgag tggcataaga cgctattacg tggtttcttc
    14401 cctctgacta ttggtggggg aattggtcaa tcacgattag ctatgttctt gcttcgtaaa
    14461 aaacatattg gagaagtaca gtcaagtgtc tggccaaaag aagtcaaaga taccttcgaa
    14521 aatatattat aaaaaatcag ccaagcagtt ttgtttggct gattttaaca ataagtttat
    14581 ttttggctta agaaagtttg gatttgttga ggtgtagctt ggcttccttg aacaagatat
    14641 ttagtcattt ttcctcgttg ataatgtgct aaatttggaa cagtggtaat atgatgcttt
    14701 ttgaaaaaac tcttcaatcg tttccctttg tatttttgac tatcaaggta gtaaattttt
    14761 gaatgaagtg tggcttgaga ttttaataat tttggtaaga aagctctaca atattggcaa
    14821 gttggacgtc ctataaaaat agtcgcttca gattgttgac ttttgcttac taaggcctta
    14881 tctgtgatgg cgattaagtc actatttgtt atcatgtgtt ggtattgttg ttgagaaata
    14941 gtcatggtgt cagcgctact taagtttgat tttagcgttg taaggcataa tatggctatt
    15001 aaagttaata ataatttttt aatcattatg ggaacctcgt ttctcttact gctagcttat
    15061 catatcaaaa aagaaggaaa agagatacta tcttctctgt ttgttataag caattttttt
    15121 gataactcta ccttattttt tgttataata gtggttatga gagtagtagc aggaacattt
    15181 ggagggcgtc ctttaaaaac gcttgatgga aaaacaactc gtccaacaac tgataaggtt
    15241 aaaggcgcta tttttaatat gattggtcct ttttttgaag gtggtcgtgt tttagacctt
    15301 ttttctggca gtggtagctt agctattgag gcgatctcaa ggggaatgga ccaagctgtc
    15361 ctagttgaaa aagataggcg tgcgcaggtc gttattcaag aaaatattgc aatgactaag
    15421 agtccggagc aatttcaatt attaaaaata gaagccaacc gtgctttaga acaattaacg
    15481 ggacaatttg atttggtctt gttggacccg ccatatgcta aggaagaaat tgtaaagcaa
    15541 atccaaatca tggatagcaa gggtttatta ggcgatgata tcatgattgt ttgtgaaacg
    15601 gacaaatcag ttgatttacc tgaagaaatt gcttcttttg ggatatggaa acagaaaatt
    15661 tatggcattt ctaaagtaac ggtctacgtt cgttaggagg ctttggtatg gattacaagt
    15721 tagtgatgat tattctcatc ttagtcatat tgatttattt gttgttgcgc cttcactatt
    15781 tagaagagtt gctaaaacat tatcacttca aaaatgatga aggagtttgt tcattatcaa
    15841 gaacaaaaaa tccattgtat ctattaatgg caatgattac ttttttgcca aatcttatca
    15901 attggatagc taacggtttt gaggcaatag acattgttcg cctgattcct gtggtagtct
    15961 ttttatggtt gtattattgt ggagatgacg acgaatgaca aagaaagctt tatttacagg
    16021 ttcatttgat cctgttacta atggacattt agatattatt gagcgcgcca gctatctatt
    16081 tgatcatgtt tacattgggt tatttcataa ccttgagaag caaggttact tttcgattga
    16141 gtgccgtaaa aaaatgttag aagaagctat ccgtcagttt aagaatgttt cagttttagt
    16201 ggcgcaagat agactagctg ttgacttagc aagggaagtg ggagcaaaat attttgttcg
    16261 aggtctgcgt aatagccagg attttgatta tgaagccaat ttggaatttt ttaataaaca
    16321 gttggcagat ggtattgaga cagtttattt atcaacgtcg ccatctttga gtccgatatc
    16381 atctagtcgc atccgtgaat tgatacattt taaggcttct gtgaagcctt ttgttccaaa
    16441 gagtgttttt agagaagtgg agaaaatgag tgaagaataa agatccaaaa agaaaacata
    16501 aaagtttgct aggtctgctt aaatggtgga ttataggttt tgctttttta ttgttagtat
    16561 tagcaagcct tgtggtgagg ctaccttatt atttggaaat gccaggtgga gcttatgata
    16621 tccgttcggt attgaaagtg aataaaaagg ctgataaagc taagggttcg tacaattttg
    16681 tagcggtatc tgtcagtcaa gcaacgccag ctcaggttct ttatgcttgg ttaactcctt
    16741 ttacagaatt atctagtaaa gaggagacaa caggtggctt tagtaatgac gattatctca
    16801 gaattaatca attctacatg gagacttctc aaaatgagtc catctatcaa gctttaaagt
    16861 tagctaataa gcaagtatct cttacttata aaggagttta tgtgcttaac ttagctaaaa
    16921 attcgacatt taaagataga ttacatttag ctgatacagt cacaggagtt aatggcaaaa
    16981 gtttcaaaaa ttcttctcaa cttatcaaat atgttgcagc tcttcatcta ggtgataaag
    17041 taaaggtaca atacacaagt caaggtaaaa agaaagaatc agttggtaaa gttattaaac
    17101 tatcaaacgg taaaaatggt attggtatcg gtttgacaga ccatacagaa gtatcatctg
    17161 atgttcctgt ggactttaat actgaaggtg ttggaggacc aagtgcaggt ttaatgttta
    17221 ctttagcgat ttacgaccaa ttagttaaag aagatttacg taaaggacgt aagattgcag
    17281 gcactggtac tattgagcaa aatgggcatg ttggcgatat tggtggagca ggtttgaaag
    17341 ttgtttcagc ggctaaaaaa ggaatggata ttttctttgt tcctaataat ccgatcgata
    17401 aaaatgctaa aaaaggcaaa acaaaggttc agacgaatta tcaagaggca aaagcagcag
    17461 ctaagcgatt aggtacgaag atgaaaattg taccagttca aaatgttcag caagctattg
    17521 attatttgaa aaaaacaaaa taaactcaaa gagttcgggc gtctgctcgg cttttttgtt
    17581 ttgcgacatt taactatcaa agtgctaaaa tgatagtatg acagagctta taagaatact
    17641 acatttaaat gatctgcact ctcattttga gaattttcct aaggtaaaac gattttttca
    17701 taacaatcaa gcgcagccaa tagagacgat ttcacttgac ttgggggata atattgataa
    17761 aagccatcct ttgacagaag caagctcggg caaggctaat gtccaattga tgaatgaact
    17821 gggtattgag cttgctacta ttggtaataa tgaaggagtt ggtttatcca aaaaagactt
    17881 ggaccaagtc tacaaagatt cagattttac ggttattgtt ggtaatctaa aagataacat
    17941 aatcgaaccg agttgggcaa aaccttacat tatttatgag acacagcaag gaactaagct
    18001 tgctttttta gcttatactt ttccttatta taaaacctat gaacctaatg gatggacgat
    18061 agaagatcca attgactgtc ttaaatgcca tttacaaata aatgagataa aagaggcaaa
    18121 ttgtcgtatt ttgatgagtc atttaggtat tcgttttgat acaaggattg ctcaagaatt
    18181 ttcagaaatt gaccttatta taggtgcaca tacacaccat ttatttgagg aaggggagct
    18241 aattaccggt acatatcttg cagcagctgg taaatatggt cgctttgttg ggagtattga
    18301 tataactttt gataatcata cactaaaaga tattttaatc tcaacatatg atacaaaaca
    18361 actaactggt tatccttcgg acagtgattg ggctaaggag attgagtcag aaggtaaaga
    18421 acagcttaga aaagaaagtt taatatcctt tccagcacca ttatcattag aagaatcgtg
    18481 tcaactagtc atggatgcga tgctgtttta cgcaggtgca gatgttgcta ttatcaattc
    18541 tggcttaatt gttcagccat ttgaaaaaga cttttcgagg aaaaatctgc atgaatcatt
    18601 acctcatcaa atgcgtctcg ctaaattgac agtgagtagt caagaacttt tagagattta
    18661 tgaaactatt tatcaacaag gtcaattttt agcgcaacaa aagattcatg gtatgggctt
    18721 tcgtggcaag tactttggag aagttttaca ttcgggattt gattacaaaa atggaaaaat
    18781 agtgtataat gagaaagata ttgatgccaa agaagaagtg attcttgtaa tcgttgatca
    18841 gtattatttt gcatcgtatt ttgagtgttt aaaaacaaaa aaagtagact tgctttttcc
    18901 tgaattactc agagatgtgg tagcagatta tttaataaat atgacactat aaatgaaaaa
    18961 tagataaata aagagtagaa gctagtcgtt tctctcaatg attttagaga aaggagtatt
    19021 atgcgaaaag aagtgacacc agagatgctt aactataata agtatcctgg cccacagttt
    19081 attcactttg aaaatatcgt taaaagtgat gatattgaat ttcaacttgt tattaatgaa
    19141 aaatcagctt ttgatgtgac tgtctttgga caacgttttt ctgagatttt attaaaatat
    19201 gattttatcg ttggcgattg gggtaacgag cagttgaggc taagaggctt ttacaaagat
    19261 gctagtacga ttagaaaaaa tagccggatt tcacgtttag aagattatat taaagagtat
    19321 tgtaactttg gttgtgctta ttttgtgttg gagaatccaa atcctagaga tattaaattt
    19381 gatgatgaaa gacctcataa gcgtcgtaag tcaagatcca aatcacaatc atcaaagtca
    19441 caaactagaa ataatcgttc ccagtcaaat gccaatgctc attttacaag taaaaagcgt
    19501 aaagacacaa aacgccgtca agaacgtcat attaaagaag agcaagataa ggaaatgacc
    19561 tctgcaaagc agcattttgt tattcgtaag aaaaaataaa aaaaggataa actgtaatcg
    19621 tttattccac catgaagaaa gaaaatatta tgcctaaaaa aactgataca cccgactata
    19681 aaccctctat ttatagctta actcgcgatg aactaatagc ttgggctatt gaacatggag
    19741 aaaagaaatt ccgtgcttca caaatctggg actggctcta taaaaagcgt gtgcaaagct
    19801 tcgatgaaat gactaacatt tccaaagatt ttattgcact tttgaatgag aattttgttg
    19861 tcaatcctct aaaacaacgt attgttcaag aatctgctga tggaactgtt aaataccttt
    19921 ttgaattacc tgatggcatg ctgattgaga cggttttgat gcgtcaatat tatggtttat
    19981 cagtatgtgt aacgactcaa gttggttgta acattggttg cactttctgt gcaagtggtt
    20041 taataaagaa acaacgtgat cttaacaatg gcgaaatcac tgctcaaatt atgcttgttc
    20101 aaaaatattt tgatgaacgt ggacaaggtg agcgcgttag tcacattgtg gtcatgggaa
    20161 taggtgaacc ctttgataac tataccaatg tattaaaatt cttgcgtaca gttaatgatg
    20221 ataatggatt agctattggt gcacgtcaca ttacagtgtc tacttcaggt ttagcacata
    20281 aaattcgtga atttgcaaat gaaggtgtac aagttaactt agcagtatct cttcatgctc
    20341 caaataatga attacgatca agtatcatgc gtattaatcg ttctttccct cttgaaaagt
    20401 tgtttgcggc tattgaatat tatattgaga cgactaaccg tcgtgtaaca tttgagtata
    20461 ttatgttaaa tggtgtcaac gataccccag aaaatgctca agagttagca gatttaacga
    20521 aaaagattcg caaattatct tatgtcaatt taattcctta caatcctgtc tcagaacatg
    20581 accagtatag tcgtagtcct aaagagcgtg tggaagcttt ctatgacgtt ttgaagaaaa
    20641 atggagttaa ttgtgttgtt cgtcaagaac atggtacgga cattgatgca gcttgcggac
    20701 aattacgttc aaacacaatg aaacgcgatc gtcaaaaagc gaaggtaggg cggtaatgct
    20761 taaaagatta tttactgaag atggggaatt gacaaagatt agtcgtcgtt tcgtttggat
    20821 gttagtggtt atctattgtc ttattattgt caggatgtgt tttgggcctc aaattatgat
    20881 tgagggggta tcaactccga atgttcagcg cttcggaaga attgtagctc ttttagtacc
    20941 atttaattct tttcgtagtt tagatcagct aactagcttt aaagagattt tttgggttat
    21001 tggtcaaaat gtagtgaata ttttactgct gtttcctctc attatagggt tactatccct
    21061 aaagccaagt ttacggaaat ataaaagcgt tatattactt gctttcttga tgtctctttt
    21121 catagagtgt actcaagttg ttttagatat tttaatagat gctaatcggg tttttgaaat
    21181 cgacgatcta tggacaaata ccttaggcgg tcctttcgcc ctatggagtt atcgaaacat
    21241 aaaaggttgg cttctaacta ttaga
//
LOCUS       DATROD010000021        21171 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.021, whole genome shotgun sequence.
ACCESSION   DATROD010000021 DATROD010000000
VERSION     DATROD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21171
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.021"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            697..1821
                     /gene="mutY"
                     /locus_tag="VBC74_000805"
     CDS             697..1821
                     /gene="mutY"
                     /locus_tag="VBC74_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEO6941771.1"
                     /translation="MWPEDRIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YSKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFQREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFSLYPFATPQKKMLETYLKQKNA"
     gene            1998..2591
                     /locus_tag="VBC74_000806"
     CDS             1998..2591
                     /locus_tag="VBC74_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6941772.1"
                     /translation="MEIGQQIIRYRKQQALSQEELAEKVYVSRQSISNWENDKTYPDI
                     HSLLLLSQIFQVSLDQLIKGDIEKMKYTITQVDKKNFERDTKVMVTLMILLMISSYPL
                     VYFLEWLGLGIFVLLSIITMTYANRVERFKKKYDVQTYKEILAVSSGKLLDEIEKREE
                     RAKLPYQKPLIVTVFFLITVATFFASRFIFTWLFHLS"
     gene            complement(2637..2951)
                     /gene="trxA"
                     /locus_tag="VBC74_000807"
     CDS             complement(2637..2951)
                     /gene="trxA"
                     /locus_tag="VBC74_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEO6941773.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(3032..3532)
                     /locus_tag="VBC74_000808"
     CDS             complement(3032..3532)
                     /locus_tag="VBC74_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO6941774.1"
                     /translation="MESYEQFYAKLSQPFRKSPQLIILLNFLLKIVTGMMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRLDFPRPYEKWNIKPLIYKDTEGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLIL
                     GLCLFI"
     gene            complement(3536..5875)
                     /locus_tag="VBC74_000809"
     CDS             complement(3536..5875)
                     /locus_tag="VBC74_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEO6941775.1"
                     /translation="MNNKILEQLEFNKVKELILPYLKTEQSQEELSELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEESRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPEISNDSTLALINVRHPLLSNPVANDLHFDQDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADHNSLVLFDELGAGTDPQEGASLAMAILEHLRLSNIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFTLVRVQEEQ
                     KVKSKQINVVKKADSSGPRARLDLRGKRYEEAMQELDNFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRNKHVKHFAYAPQNAGGSGATIVTLG"
     gene            complement(5960..6502)
                     /locus_tag="VBC74_000810"
     CDS             complement(5960..6502)
                     /locus_tag="VBC74_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEO6941776.1"
                     /translation="MLSLLLLIIVIWHFYIGYSRGIFLQVFYVLMSMVSLMIASQFYQ
                     ELASQITLWVPYSNPVQGVEVYFFKDISKFQLSHVYYAGVAFVFIYSLSYLVGRLLGV
                     LLHLAPVEHFDSLQNNIISGFLAVLVCLLFMSMCLTILATVPMSFVQEKLWNSLFVRF
                     LINDLPFFSQFLVRTWAVGL"
     gene            complement(6505..6816)
                     /locus_tag="VBC74_000811"
     CDS             complement(6505..6816)
                     /locus_tag="VBC74_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941777.1"
                     /translation="METKINNSQKNRYKFVFGDKPLTLTTDKDNLFMEEIERVATEKY
                     EAIKEKLPNADNETIAILMAINALSVQLSREIDIEKMEDELNKLRSKTISDIKEKVSE
                     D"
     gene            6927..7820
                     /locus_tag="VBC74_000812"
     CDS             6927..7820
                     /locus_tag="VBC74_000812"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEO6941778.1"
                     /translation="MNTIVMQADKKLQEKIRTDLAQHHISNNNPYVVFSAKISGATVL
                     LYTSGKLVFQGSNASHIAQKYGFIEQKESCSSESQDIPIIGTDEVGNGSYFGGLAVVA
                     SFVTPKDHAYLKKLGVGDSKTLTDQKIKQIAPLLEKAIPHKALLLSPQKYNQVVSPNN
                     KHNAVSVKVALHNQAIFLLLQDGFEPEKIVIDAFTSSKNYQNYLKNEKNQFKQTITLE
                     EKAENKYLAVAVSSIIARNLFLENLNKLSDDVGYKLPSGAGHQSDKVASQLLKAYGIS
                     SLEHCAKLHFANTKKAQALLK"
     gene            7836..8429
                     /gene="lepB"
                     /locus_tag="VBC74_000813"
     CDS             7836..8429
                     /gene="lepB"
                     /locus_tag="VBC74_000813"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO6941779.1"
                     /translation="MKEFIKEWGVFILILSLFLLSRIFLWQFVKVDGHSMDPTLADKE
                     QLVVLKQTKINRFDIVVANEEEGGQKKKIVKRVIGMPGDVIKYKNDTLTINNKKTEEP
                     YLKEYTKLFKKDKLQEKYSYNPLFQDLAQSSTAFTTDSNGSSEFTTVVPKGHYYLVGD
                     DRIVSKDSRAVGSFKKSTIVGEVKFRFWPIRRFGTIN"
     gene            8558..10978
                     /locus_tag="VBC74_000814"
     CDS             8558..10978
                     /locus_tag="VBC74_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEO6941780.1"
                     /translation="MEVFFTGTIERIIFENASNFFKILLLEIEDTDSDFDDVEVIITG
                     TMADVIEGEEYTFWGTLTQHPKYGEQLQSVRYERAKPTSGGLVKYFSSEQFKGIGKKT
                     AQRIVELYGDNTIDKILESPEQLSTISGLSKINREAFIAKLKLNYGTEQVLAKLAEYG
                     LSNRAAIQIFDHYKEESLEVINENPYQLVEDIQGIGFKIADQLAEQVGIESDSPKRFR
                     AAIIHTLVESSMEQGDTYIEARTLLEKTITLLEEARQIELDPSIVAKELTNLIAEDKV
                     QHIGTKIFSNTLFFAEEGIKKNLQRILNQPLDKQLNHKDIDREIRDIQKSLNIHYDNI
                     QEKAIREALLSKVFILTGGPGTGKTTVINGIIEAYSELHHIDLNKNDIPIVLAAPTGR
                     AARRMNELSGLPSATIHRHLGLNGDSDYQSLDDYLDCSLIIIDEFSMVDTWLANQLFD
                     ALDSHTQVIIVGDSDQLPSVGPGQVLADLLNINALPHVKLEKIFRQSEESTIVTLANQ
                     MRQGFLPEDFTAKKADRSYFEASANIIPNMISKIVQSALKSGIEAHEIQILAPMYRGQ
                     AGINNLNLIMQNLLNPLKDNNQFTFNDINFRIGDKVLHLVNDTELNVFNGDIGYITDL
                     IPAKYTESKQDEIYMTFDGQEVIYQRKEWLKITLAYAMSIHKSQGSEFQVVILPITRQ
                     SGRMLQRNLIYTAITRSKSKLILLGEIGAFDFAVKNEGAKRNTYLIERFENKQEIANS
                     QKIEDSSIDQKIDNTIINTSIPKTATPIEQTNLSKITYRLTEENYLTIDPMIGINQQD
                     ISAIFDSK"
     gene            11092..11574
                     /locus_tag="VBC74_000815"
     CDS             11092..11574
                     /locus_tag="VBC74_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941781.1"
                     /translation="MVSYEKVRRSLRTATITIIVLNSLSLVFRLFTGISVQLAKTEIN
                     KGNTGNLPKEHIEAVLSATTPFMLFVTALIVLVNIAIVIFCIKNLRAIKRNQMVNYLP
                     YYLGFAITVGLVILGFLTTKAPWAIAINIVFQAIFGLLYFHAYQKAQKLNERDLEETN
                     "
     gene            complement(11645..12754)
                     /gene="dinB"
                     /locus_tag="VBC74_000816"
     CDS             complement(11645..12754)
                     /gene="dinB"
                     /locus_tag="VBC74_000816"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEO6941782.1"
                     /translation="MGERTMLIFPLINDTSRKIIHIDMDAFFASVEERDNPSLKGKPV
                     IIGSDPRKTGGRGVVSTCNYEARKFGVHSAMSSKEAYERCPQAIFISGNYQKYRQVGM
                     EVRDIFKKYTDLVEPMSIDEAYLDVTENKMGIKSAVKLAKMIQYDIWNDVHLTCSAGI
                     SYNKFLAKLASDFEKPKGLTFILPDQAQDFLKPLPIEKFHGVGKRSVEKLHALGVYTG
                     EDLLSLSEISLIDMFGRFGYDLYRKARGINASPVKPNRVRKSIGSEKTYGKLLYNEAD
                     IKAEISKNVQRVVASLEKNKKVGKTIVLKVRYADFETLTKRMTLEEYTQDFQIIDQVA
                     KAIFDTLEESVFGIRLLGVTVTTLENEHEAIYLDF"
     gene            12924..15236
                     /gene="pflB"
                     /locus_tag="VBC74_000817"
     CDS             12924..15236
                     /gene="pflB"
                     /locus_tag="VBC74_000817"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEO6941783.1"
                     /translation="MATVKTNTDIFEQAWEGFKGVDWKEKASIARFVQANYAPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEETRFPMDTRVASISELPAGFIDKDNELIFGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYATTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLAVYGADYLMQEKVNDWNALNDIDEESIRLREEI
                     NLQYQALGEVVKLGDLYGVDVRKPAMNTKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFARTKAYDALYSGDPTFITTSMA
                     GMGADGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWSDQLPYAFRRYCMSMSHKHSSI
                     QYEGVSTMAKEGYGEMSCISCCVSPLDPENEDKRHNLQYFGARVNVMKALLTGLNGGY
                     DDVHKDYKVFDIDPIRDEVLNFDTVKANFEKSLDWLTDTYVDAMNIIHYMTDKYNYEA
                     VQMAFLPSHVRANMGFGICGFANTVDSLSAIKYATVKPIRDEDGYIYDYETVGDFPRY
                     GEDDDRVDSIAEWLLEAFHGRLAKHKLYKDAEATVSLLTITSNVAYSKQTGNSPVHKG
                     VYLNEDGSVNLSKVEFFSPGANPSNKAKGGWLQNLNSLSKLDFAHANDGISLTTQVSP
                     RALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLKDVYDKIMNGEDVIVRISGYCV
                     NTKYLTPEQKTELTQRVFHEVLSMDDALTN"
     gene            15337..15710
                     /locus_tag="VBC74_000818"
                     /pseudo
     CDS             15337..15710
                     /locus_tag="VBC74_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
     gene            complement(15745..16674)
                     /locus_tag="VBC74_000819"
     CDS             complement(15745..16674)
                     /locus_tag="VBC74_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEO6941784.1"
                     /translation="MIKTNTVLELIKEQIKDNLYHGASLAIYENGEWHEHYLGTIDGN
                     EKVKAGLVYDLASVSKVVGVGTLLAKLVYQGTIDIDKPLRYYYPTFHHQTLTVRQLAT
                     HSSGIDPFIPNRDQLNATQLKDAINHIKVLEDKSFKYTDINFLLLGFMLEEVLGDSLD
                     KLFKRYIFTPFQMKETSFGPRVEAVPTVAGINDGIVHDPKAKVLGKHTGSAGLFSTID
                     DLQRFSIHYLKDDFAKPLWNNYSLSKSRSLAWDIDKDWINHTGYTGPFIALNYQKQAA
                     AIFLTNRTFSYDDRPLWIKKRRHVQEAITSYFE"
     gene            complement(16671..17426)
                     /locus_tag="VBC74_000820"
     CDS             complement(16671..17426)
                     /locus_tag="VBC74_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEO6941785.1"
                     /translation="MTLFHSLTFKHPVLRVNNRDLNIAFYQESLGFKLISEENAIAVF
                     SAWQNKEASFIIEESPTYRTRAVNGTKKLAKIIVKSQDAKDIEKLLANGAQAIQVYQG
                     QNGYAYETVSPEGDLFLLHAEDDLSQLVAIERPELEKKDDTTGLSNFAFQSISLNVPD
                     AVKAEAFYDKVFAGKFPINLSFKEAQGQDLQIAPNETWDIEILECCVNEDTNLNDLKS
                     TFESWGLDVYLDSKEKILVISDTSNIEIWISKE"
     gene            17551..18447
                     /locus_tag="VBC74_000821"
     CDS             17551..18447
                     /locus_tag="VBC74_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEO6941786.1"
                     /translation="MNETLLLHGIQLILIIAMIITFYQIVRHIRSQKINPFKRFFTGL
                     WIGFVTDALDTLGIGSFATTTTFFKLTKLVEDDRKIPATMTAAHVLPVLLQSLCFIFV
                     VKVEALTLITMAGAAFIGAFVGAKMTKNWHAPTVQRILGTLLITAAIIMLYRMITNPG
                     AGISDSVHGLHGIWLFVGIVFNFIIGVLMTMGLGNYAPELIFFSLMGLSPAVAMPVMM
                     LDAAMIMTASSTQFIKSGRVNWNGFAGLVTGGILGVIVAVLFLTNLDLNSLKTLVVGI
                     VLFTGAMLIRSSFKKTMPKSHH"
     gene            complement(18526..19374)
                     /locus_tag="VBC74_000822"
     CDS             complement(18526..19374)
                     /locus_tag="VBC74_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEO6941787.1"
                     /translation="MIEITWTVKYITEFIATAFLIILGNGAVANVDLKGTKGNNSGWI
                     IIAIGYGLGVMMPALMFGNVSGNHINPAFTLGLAFSGLFPWAHVGQYILAQILGAIFG
                     QLVVVMVYQPYFVKTENPNHVLGSFSTISALDDGQKSSRKAAYINGFLNEFVGSFVLF
                     FGALALTKNYFGVELVGKLVQAGYDQTTAATRISPYVTGSLAVAHLGIGFLVMTLVAS
                     LGGPTGPALNPARDLGPRIVHRLLPKQILGQAKEDSKWWYAWVPVLAPIVASILAVAL
                     FKLLYL"
     gene            complement(19542..19994)
                     /locus_tag="VBC74_000823"
     CDS             complement(19542..19994)
                     /locus_tag="VBC74_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEO6941788.1"
                     /translation="MTQKYERILIAIDGSYESELAVEKGINVALRNDAELLLTHVIDA
                     HAYQSEGVFSDYVFDRQEQESADVLAYFEKLAHSKGLTKIKKITEIGNPKTLLAKDIP
                     IREKADLIMVGATGLNTFESLLIGSTSEYILRHSKVDMLVVRDSKKTL"
     gene            complement(20012..>21171)
                     /locus_tag="VBC74_000824"
     CDS             complement(20012..>21171)
                     /locus_tag="VBC74_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6941789.1"
                     /translation="LNFIVYMVYYLFTVIIAFIATKELGVSTSQAGLATGIYIVGTLI
                     ARLIFGKQLEVLGRKLVLRGGAIFYLLTTLAYFYMPSIGVMYLVRFLNGFGYGVVSTA
                     TNTIVTAYIPADKRGEGINFYGLSTSLAAAIGPFVGTFMLDNLHINFKMVIVLCSILI
                     AIVVLGAFVFPVKNITLNPEQLAKSKSWTIDSFIEKKAIFITIIAFLMGISYASVLGF
                     QKLYTTEINLMTVGAYFFIVYALVITLTRPSMGRLMDAKGDKWVLYPSYLFLTLGLAL
                     LGSAMGSVTYLLSGALIGFGYGTFMSCGQAASIKGVEEHRFNTAMSTYMIGLDLGLGA
                     GPYILGLVKDGFLGAGVQSFRELFWIAAIIPVVCGILYFLKSSRQVETKTI"
BASE COUNT         6995 a         4058 c         3450 g         6668 t
ORIGIN      
        1 acctttggta acaccaaaac atacacgaac ttttattcat atgtgttacc attttacatc
       61 tttcatattg agatatttga ttagcaatac ctccttgact aacaaaaatt actaacacat
      121 ctaggggtat accctagttt atatttgttc atgattacct caagcgagca tgcgctaatg
      181 gtaatgaact attaccacat tgtctgtctt ccttaaatta tataaggact tatgtgtcac
      241 cgttactgta gcgacctaca tctaaaccta acataagtgc ccctccaact atttctagga
      301 aatgaagaaa acttcaaatc cttgtgactg tttgcaacat caatgcattg ataacaattt
      361 tagtgtatcg ccattcttat agcgacccat attaaaaatt aacataagcg ctacctccat
      421 taactaaagt tagcttgcct accaatcttt cttttggaga ctagtctcca ttcttatacc
      481 tagagatatt gtaatagatc atggataacc tccttgattt aaactttatc tttataattt
      541 aattatagtg taaacgcttc cttaagtcaa gaaaaaaata ttgatgcaac atttttcttc
      601 atcattaagt acgatctagt caacatacct ctttgtcaaa tcctctttct ttgatacaat
      661 taatctatga tagatttaaa agactatgga attgagatgt ggcctgaaga tagaatcgct
      721 tcttttcgtc gcacactttt agggtggtat gaccaagaaa aacgtgatct cccttggcga
      781 agaactacta atccatacta tatttgggtt tctgaaatca tgttgcaaca aacacaagtt
      841 aatacggtaa tcccttatta taaacgtttt ctagagtggt ttcctcaaat aaaagatttg
      901 gcagacgctc ctgaagaaca attactaaag gcatgggagg gactaggcta ttattctcgg
      961 gtgcgtaata tgcaaaaggc tgcacagcaa gtgatggttg attttggtgg catatttcct
     1021 cacacttatg atgatattgc ttcattaaaa gggattggtc cttatactgc aggtgctatc
     1081 gccagcatct cattcaactt accagagcct gctgttgatg gcaatgtgat gcgtgtcatg
     1141 gctcgtttgt ttgaagttaa ttatgacatc ggtgacccta aaaatcgtaa aatttttcaa
     1201 gctattatgg aaatactaat tgaccctgac agacccggtg attttaacca agcacttatg
     1261 gatctgggta cagacatcga atccgctaag acacctagac ctgatgaaag ccctattcgt
     1321 ttctttaatg ctgcttattt gaatggtact tatagtaaat atcccataaa aaatcctaag
     1381 aaaaagccta aaccaatgcg aattcaggct tttgtcattc gtaaccaaaa tggtcaatat
     1441 ctactggaga aaaatactaa agggagactt ctagggggtt tttggtcatt tcctattatc
     1501 gaaacgtcac ctctttctca acaattagat ttatttgatg acaatcaatc caaccccata
     1561 atctggcaaa cccaaaatga aactttccaa agagaatatc aattaaagcc ccaatggact
     1621 gataatcact ttccaaatat taagcacact tttagtcacc agaagtggac aatagaactc
     1681 atagaggggg tggtcaaagc aacagattta cctaatgctc ctcacctaaa gtgggtagca
     1741 attgaggact tttctctgta tccctttgca acaccacaaa agaaaatgct agagacctac
     1801 ttaaaacaga aaaatgctta atcacaagtg aactatgacg gttcactttt tgtttacaat
     1861 tcctttacgc cgctgtaaag ttttcctcag attatctttt aataccctat aaatagtggt
     1921 ctatccctaa tgtcaaatac atttgataga cccgttagct tatcatcctt atactaatat
     1981 tatgaaagga gatatttatg gaaattggtc agcaaattat tcgctaccgt aaacaacaag
     2041 cgctctcgca agaagaatta gccgagaaag tctatgtaag ccgtcagagc atatctaact
     2101 gggaaaatga caaaacttac ccagacatcc atagcctctt actcctaagt caaatttttc
     2161 aagtgagctt agaccaacta attaaaggag acattgagaa aatgaaatat accatcactc
     2221 aggttgataa aaagaatttt gaacgagata ccaaagtaat ggtgacattg atgatactat
     2281 taatgatttc aagttatcca ttagtctatt tcttagagtg gctaggactt ggcatttttg
     2341 ttttattatc gataattact atgacttatg ctaatcgtgt ggaacgtttt aagaaaaaat
     2401 atgatgtgca aacttataag gaaatcctag ctgtttcaag tggaaaatta ttagacgaaa
     2461 tcgaaaaacg ggaagaaaga gcaaaacttc cttatcagaa accccttatt gtcactgtat
     2521 ttttcctcat tactgttgcc accttttttg caagtcgttt tattttcaca tggttgttcc
     2581 atttaagtta gtagataata aaataaagct cagcgtgaca ctgggcttta ttttatttaa
     2641 ctcaattcag caataatagc tttcagttgg tctttagtat gaacacctgc aacttgttta
     2701 accacttctc cgtctttttt aaacatcaat gttggaattg acataatacc aaactgacgg
     2761 gcagtttccg gattctcatc aacatccatt ttcaaaattt tgagttcatc ttcatcgatt
     2821 tcttgagata actgttctaa gattggtgct tgcatacgac atggaccaca ccaagttgcc
     2881 caaaaatcaa tgagtactaa accttcttta gtttcttcta caaatgtagc atctgttact
     2941 tctaatgcca tcttttttct ccatttcatc ttaatgataa gcctattgta caagaattta
     3001 gttgtttttg aaactatttg ctacgaactc gctaaataaa taaacaaagc cctaaaatca
     3061 agccaataag gtaacctaca ataacatcct taggatagtg aatacctgct atcacacggc
     3121 aaattgccaa tagaaccgat aatattaaac aaactattcc aaagtaaaca taatatcgta
     3181 acaaacacat actaatcatc gtagctgaaa aaacatgtct gctaggcatt gaccttccct
     3241 cagtatcctt ataaattaaa ggtttaatat tccatttttc atatggtcgt ggaaaatcaa
     3301 ggcgtttcct aatgtaagac aaagctataa agccaacagc agggataatt aatagcctta
     3361 accataattg aaatgtcatc ccttgccaaa gagtaaatat cagaaaagat ggatagagta
     3421 tgtacatcat tcctgtgact atttttagta aaaaatttaa aagaattatt aactgtgggg
     3481 attttctaaa aggttgagat agtttggcat aaaattgttc atatgattcc ataatttacc
     3541 ctaacgttac aattgtggcg ccagatcccc ctgcattttg tggggcataa gcaaaatgct
     3601 taacgtgctt attacgacga agatattttg tcactccctc acggataacg cctgtaccaa
     3661 taccatgaat gatatcaact tgtcccatat tgttaagcaa tgcttgatca ataaaattat
     3721 ctaactcttg catagcttct tcgtatcttt tacctctaag atcaagtcga gctcttggtc
     3781 cagaactatc agccttttta accacattaa tctgtttact tttgactttc tgttcttctt
     3841 ggactctaac aagggtaaat tcatcttgtg ttaatgtcat tttgataatt cccacttgtg
     3901 cttcccaacg tccatctttt aattgactag ttaaggtacc tcgctgtcca tagctagtca
     3961 ctataatatc atcaccaatt ctaggagcac gagctgcttt aattttttta gccttattta
     4021 agactttatt ttttgataaa tcaacttgag gtgctaattt ttttatttgt gccttagcat
     4081 ctataatttc gtgaggtttt aattggctct tatcattgag tttttttaag atagtatcac
     4141 tctcattcaa agccatatct acaatttctt gagcttcttg atagattttt tctaactctt
     4201 tatcgcgctc atgtgaaaat tcattataga gtttcttaac cgcacgattg aatttgaggt
     4261 tttcttgttc aacttcttta atatgatcca gtcttctacg tgtctcaagt gtctgtgcct
     4321 ctaactgttc aataatacgg ttaacatctg agtcagaatc tgtcatctgc ttagcttgtt
     4381 taacaataaa tggagctaaa ccaaggcgag aagcaatttc aaatgcattt gatcgtccag
     4441 gaactccttg cataaagcga tacgtagggc taagcgtttc ggcatcaaat tccatgctcg
     4501 cattctctac aaaatttgtc tcaatcccat aagcttttaa ttctggatag tgcgtggtcg
     4561 ccatcgtttt gatattactt aacctaaggt gttctaaaat agccatagcc aaactagcac
     4621 cttcttgagg atccgttcct gctcccagtt catcaaagag gactaaacta ttgtggtcag
     4681 cctcgtttaa aatactgact atgtgcgtca tatgactaga aaaagttgat agactttgtt
     4741 caatagattg ctcatcgcca atatctgcaa agatattgtt aaatactgca attttactac
     4801 ctttatccgc taatactggc aaaccagact gtcccattaa ttgtgctaaa ccgagtgttt
     4861 ttagcataat cgtcttacca ccagtattgg gaccagtgat gacaattgca gttaaatctt
     4921 gatcaaaatg taagtcatta gccacagggt tacttaacag aggatgacga acattgatta
     4981 atgctaacgt gctgtcatta gaaatctcag gtatcgtcgc cttattatca gacataaaaa
     5041 gatatttagc ccttacaaaa tcaagatgcc caagaatcca tgcattattt ctaatagtgg
     5101 cgacatgggg tcttaacaag tctgaaaatg cgtgtaaaat acgactttct tcatgacgtt
     5161 cgtcagctct aagctgcgtt atctcttcgt ttagtgtaac tacagcacga ggctcaatat
     5221 aaacagtact tcctgaagaa gagatgtcat gaaccacacc agaaatacga ttccgataag
     5281 tattttttac tggtaggaca cttcgtccac tacgactagc gattagattc tctgataaaa
     5341 gctctgcttt ttccttaagc atatcctgta aaatctgacg aacccgtcgt tcactgtttg
     5401 ttaattgacg acggatacgc tctaattctg gactcgcaaa atgttctaaa aaaccaccat
     5461 cattgatagc ttgaaaagac ccttgcagat tagggaattg ttccaaattt tcaaacaaac
     5521 gatccaaaga ttggaaagaa acattatcca aatcagaata aaagtgaatc atatccgaag
     5581 aactctgtaa aacttttttg atagccaaaa gttcttgaat attaagatca gctgaaagct
     5641 ctaagcgttt taaactctca gagattgaac ttaggctgac tatgccaaat gagtgatgtt
     5701 caacaaaaat ctgttccatg tcagaaattt cattaaaact tttttctatt ttaggagcct
     5761 ctgtcatcgg ctccagctct gataattctt cttgtgattg ttctgtcttg agataaggta
     5821 atatcaattc cttaactttg ttaaattcta actgttctaa aatcttgtta ttcatactct
     5881 tcattatacc taaatttatc acaaaatgac aacgttaaaa aggttggaac tcttactcca
     5941 accttactaa acattatatt caaagaccta ccgcccaagt ccttaccaaa aactgtgaga
     6001 agaatggtaa gtcgttaatt aaaaatctaa caaacagact attccataac ttttcttgaa
     6061 caaatgacat agggactgtt gccagaatag tcaaacacat actcataaat aagagacaaa
     6121 ccagaacggc taaaaaacca ctaataatat tattttgcaa gctatcaaaa tgttcaactg
     6181 gtgctaaatg tagtaaaaca cctaacaagc gacctactaa gtaagataaa ctgtagataa
     6241 aaacaaaggc aacacctgca taataaacat gactcaattg aaatttgcta atatctttaa
     6301 aaaagtaaac ttctactcct tgtacaggat tagaatacgg tacccataac gtaatctgac
     6361 tagctaattc ttggtaaaat tgactagcaa tcatcaaaga taccattgac attaacacgt
     6421 aaaagacttg taaaaaaata cctctactgt agccaatata aaaatgccaa ataacaataa
     6481 tcagcaaaag caatgatagc atgattaatc ctcactcact ttttctttaa tgtccgaaat
     6541 cgttttggag cgtaacttat tgagctcatc ttccattttt tcaatgtcaa tttctcgact
     6601 tagttgcact gataaagcat taattgccat gagaatagca atcgtttcat tatcagcatt
     6661 aggtaatttt tctttaatag cttcatactt ttctgtagct acacgctcaa tctcttccat
     6721 aaaaaggttg tctttatctg tcgtcaatgt gagaggctta tctccaaaga caaatttata
     6781 acgatttttc tgactattat ttatttttgt ttccatacta tacctctgct agcattatac
     6841 cgtaaaatag ggctttcgta aaattctacc ccttatcatt acttgtctat tcccttattc
     6901 ctctttttta tgatagaata ttgtctatga acacaatcgt catgcaagca gataaaaaat
     6961 tgcaagaaaa gataagaacc gatttagctc aacatcatat aagtaataac aatccttatg
     7021 tggtattttc agctaaaata tcaggagcta ctgttctttt atatacttct ggaaaacttg
     7081 tcttccaggg ttctaacgct tcacatatcg cccaaaagta tggttttatt gagcaaaaag
     7141 aaagttgttc ctctgagagt caagatattc ccataattgg tactgatgaa gtgggaaatg
     7201 gctcttactt tggtggactg gcagtggtag ctagttttgt aactcctaag gaccatgctt
     7261 acctcaaaaa attaggtgtt ggtgattcaa aaacacttac tgatcaaaaa attaagcaaa
     7321 tcgcgcctct tttggaaaaa gcaattccac acaaagcatt attgttatcg cctcaaaaat
     7381 ataatcaagt ggttagtcca aataacaaac ataatgctgt ctccgtaaaa gttgctttac
     7441 acaatcaagc tatcttcctt cttcttcaag atggttttga accagaaaaa atcgttattg
     7501 atgcttttac tagctccaaa aactatcaaa attatttgaa aaatgaaaag aatcaattta
     7561 aacaaacgat tactctggaa gaaaaagctg aaaataaata tctagctgta gcggtcagtt
     7621 ctattattgc acgtaatcta ttcttagaaa accttaacaa attaagcgac gacgtaggat
     7681 ataaacttcc aagtggagct ggtcaccaat ctgataaggt cgctagccaa ttactcaaag
     7741 cttatggtat ctcttcgctt gaacactgcg caaaacttca ttttgccaac accaaaaaag
     7801 cacaagcatt attaaaataa atagcaggga aaattatgaa agaatttatt aaagaatggg
     7861 gtgtctttat cctcatcctc tcactttttt tactatcgcg tatcttttta tggcaattcg
     7921 ttaaagttga cggacactcc atggatccaa ctttagctga caaggaacag ctagtagttc
     7981 tcaaacaaac aaaaatcaat cgattcgata ttgtagtggc taacgaagaa gaaggcggcc
     8041 aaaagaaaaa aattgttaaa cgtgtcattg gtatgccagg tgatgtcatc aaatataaaa
     8101 atgacacctt aactattaac aataaaaaaa cagaagaacc ttacctcaag gaatatacta
     8161 aattatttaa aaaggataaa ttacaggaaa aatattcgta taacccactt ttccaagacc
     8221 tagcacaaag ctctaccgct ttcaccactg acagcaatgg cagcagcgaa tttactactg
     8281 tcgtgcctaa aggccactac tatcttgttg gtgatgaccg aattgtctct aaagatagtc
     8341 gtgccgtcgg ttccttcaaa aaatcaacga ttgtgggaga ggttaaattc cgcttctggc
     8401 caattcgtcg ttttggaact atcaactaaa ggaaattaaa agttcacctg acaaaagttg
     8461 aaatagcata ttatattgaa aatagggagt gacaaatcca tttaaaatag ggatttacaa
     8521 tcactccctt taactgttta ttaaaaagga aggtacaatg gaagtatttt ttacaggaac
     8581 cattgaacgc attatatttg aaaatgccag caattttttt aaaatattac ttctggaaat
     8641 cgaagatacg gatagcgatt ttgatgatgt agaagtaatt attactggta ctatggccga
     8701 cgttatagag ggagaagaat atactttttg gggaacacta acccaacatc caaaatacgg
     8761 cgagcaacta caaagtgtac gctatgaaag agccaagcct acttcaggtg gccttgtgaa
     8821 atacttttct agcgaacaat ttaaaggaat tggtaaaaag actgcccaac gcatcgtaga
     8881 actctatgga gataatacca ttgataaaat ccttgaatcc cctgaacagt taagtacaat
     8941 ctcaggacta tcaaaaatta atagagaagc attcatcgct aaactgaaac tcaattatgg
     9001 aacagagcaa gttttagcta aactcgctga atatgggtta agcaatcgtg ctgctattca
     9061 aatatttgat cactataaag aagaatcact tgaagtgata aacgaaaatc cttatcaact
     9121 tgttgaggat atccaaggta tcggttttaa aatagctgac caacttgctg aacaagtcgg
     9181 tattgaaagt gattctccta aacgctttcg tgctgctatt attcatactt tagttgagtc
     9241 ttctatggag caaggagata cctatattga ggctcgtact ctcttggaaa aaaccattac
     9301 tctccttgaa gaagctcgtc aaatcgaact agatccttca attgttgcta aggaattgac
     9361 aaatttaatt gcagaagata aagttcaaca tattggaact aaaattttta gtaatactct
     9421 attttttgcc gaagaaggca tcaaaaaaaa cttacaacga atactaaatc agcctcttga
     9481 taaacaacta aatcacaaag atattgaccg tgaaatcaga gatattcaaa aatctttaaa
     9541 tatccactac gataatatcc aagaaaaagc tattagagaa gctttactaa gtaaggtatt
     9601 tatcttgact ggtggcccag gaacaggtaa aacaacagtt ataaatggta ttattgaagc
     9661 ctattccgag ttacatcata ttgatttaaa caaaaatgat attcctattg tacttgctgc
     9721 tcctacaggt cgtgctgcta gacgcatgaa tgaacttagc ggtttaccga gtgctactat
     9781 tcataggcat ttgggattaa acggagatag tgactatcaa agtctagatg actatctaga
     9841 ttgttcctta attatcattg atgaattttc aatggttgac acttggcttg ctaatcaatt
     9901 atttgacgct ttggactcgc acacacaagt tattattgtc ggagatagtg atcaattacc
     9961 gtctgtaggt ccaggacaag ttctagcaga tcttttaaat ataaatgcac ttccacacgt
    10021 taaacttgaa aagattttta gacaatcaga agaatcaact attgtgactt tagctaatca
    10081 aatgagacag ggctttttgc cagaggactt tacagctaaa aaagccgatc gctcatactt
    10141 tgaagcatct gctaatatca tcccaaatat gatttctaaa attgttcaat ctgcacttaa
    10201 gagcggtatt gaagctcatg aaattcaaat tttagctcct atgtatcgcg gccaagcagg
    10261 tattaataat cttaacctta tcatgcaaaa tctcctcaat ccattaaaag ataataacca
    10321 atttactttt aatgatataa atttccgaat aggagataaa gtgttacatt tagtcaacga
    10381 tacagagctt aatgttttta atggggatat tggttacata acggacctta taccagccaa
    10441 atatactgag agcaagcaag atgagattta tatgactttt gacggtcaag aagttatcta
    10501 tcaacgcaaa gaatggctta aaataacact agcctatgct atgagtattc ataaatcaca
    10561 gggttctgaa tttcaagttg ttattctgcc aataacgaga cagagtggtc gaatgttaca
    10621 gcgaaacctt atttatacag ctattacgag atctaaaagt aaactaatac ttttaggaga
    10681 aattggtgca tttgatttcg ctgttaaaaa cgagggggca aagcgcaata cttatctgat
    10741 agaaagattt gaaaacaagc aagaaatagc taattctcaa aaaattgaag atagctcaat
    10801 agatcaaaag attgacaaca caataattaa tacgtccatt ccaaaaacag caacaccaat
    10861 tgagcagact aatctttcaa aaataactta tcgcctaact gaagaaaatt atttaacaat
    10921 cgatccaatg attggtatta atcaacaaga tataagtgca atttttgata gtaaataaat
    10981 tttttcataa aatcttgaaa actcttccaa atatgttata atatcattgt attaagaacg
    11041 tagttcaaat ttaatacttt tattaaggtt tatttagaaa aaggagatat tatggtttca
    11101 tatgaaaaag tccgccgctc tctgcgcaca gctacaatca ctatcattgt attaaatagt
    11161 ttgtctttag tattcagatt gtttacagga atttcagtgc agctagccaa aactgaaatt
    11221 aataaaggga acacaggtaa tcttcctaaa gaacatatcg aagcagtctt gagcgccaca
    11281 acaccattta tgctatttgt cactgcttta atagtattag tcaacattgc cattgttatt
    11341 ttctgtatta aaaatttaag agcaattaaa agaaatcaaa tggttaatta ccttccttat
    11401 taccttggct tcgccattac tgtaggactt gttattttgg gttttctaac tactaaagca
    11461 ccatgggcaa tagctataaa tatcgtcttc caagctattt tcggtcttct ttatttccat
    11521 gcttatcaaa aagcacaaaa actcaacgaa cgtgatcttg aagagactaa ctaaaagtaa
    11581 aagcactaga atctagtgct tttttctttt caaagagagt taaatatagt catcgtccca
    11641 acttttaaaa atctaaatag atagcttcat gttcattttc cagggtcgtt actgtaacac
    11701 ctaagagtcg tatgccaaaa actgactctt ctaaggtatc aaatatcgct ttagcaactt
    11761 gatcaatgat ttgaaaatct tgagtatact cttctaatgt catacgtttc gtcaatgttt
    11821 caaaatccgc atatctaact tttaaaacga ttgtcttacc aactttctta tttttctcca
    11881 gagaagcaac aactctctga acgttctttg aaatctcagc tttgatatct gcttcgttat
    11941 atagtaattt cccataggtt ttctcacttc ctattgattt acgaactcta ttaggtttta
    12001 ccggggaagc attaatccct cttgccttac gataaaggtc atacccgaaa cgaccaaaca
    12061 tatcaattaa agaaatttca gataaggaaa ggaggtcctc accagtataa acccctaaag
    12121 catgtaattt ttcgacggag cgtttaccaa caccatgaaa tttctctatt ggtaagggct
    12181 tcaagaaatc ttgagcttga tctggtagga tgaaggtaag tccttttggt ttctcaaagt
    12241 cagaagccaa ttttgccaaa aatttattgt aggaaatacc cgctgaacag gtcaaatgga
    12301 catcattcca aatatcgtat tgtatcattt tagcaagttt aacagctgat tttattccca
    12361 ttttattttc agttacatct aaataagctt cgtctatact catcggttca accaaatccg
    12421 tatacttttt aaatatatct cgtacttcca ttcctacttg gcgatatttt tggtaatttc
    12481 ccgaaataaa gatagcttga gggcatcgtt catacgcttc ttttgaagac atagctgaat
    12541 gaactccaaa ctttcgagcc tcataattac aagtagaaac aacccctctg ccgccggttt
    12601 ttctgggatc agaaccaata ataacaggct ttcctttaag agagggattg tcacgctctt
    12661 ccacagatgc aaaaaaagca tccatatcaa tgtggataat tttacgcgac gtatcattta
    12721 ttaaaggaaa aattaacatt gttctctccc ccattctctc tactccatta taacagatta
    12781 caacttaatg atacagatta aaaaaactgt attataaaag aatcataatt ttgtcaagaa
    12841 atatgcttgc gaaaacgttt actttttcct aaaatggata tgtaaacaaa attaaatctg
    12901 tcacaatgtt taaggagaaa actatggcaa cagttaaaac aaacacagat atctttgaac
    12961 aagcttggga aggctttaaa ggtgttgatt ggaaagaaaa agcaagtatt gctcgcttcg
    13021 ttcaagcaaa ctatgcacct tacgatggag atgaaagctt cttagctgga gcaactgaac
    13081 gttcacttca catcaaaaaa gtgattgaag aaacaaaagc tcactatgaa gaaactcgct
    13141 tcccaatgga tactcgtgtt gcttcaattt ctgaattacc agctggtttt atcgacaaag
    13201 ataacgagct tatctttggt attcaaaacg acgagctttt caaattgaat ttcatgccta
    13261 aaggtggtat ccgtatggcg gaaactacac ttaaagaaaa tggttatgaa cctgatccag
    13321 ctgttcacga aatcttcact aagtacgcta caacagtaaa tgatggtatc ttccgcgctt
    13381 atacttctaa cattcgtcgt gctcgccatg cacacactgt tactggtctt ccagacgctt
    13441 attcacgcgg acgtatcatt ggtgtatatg ctcgtctagc tgtttacggt gctgactact
    13501 tgatgcaaga aaaagttaat gactggaatg cattgaatga tattgatgaa gagtcaattc
    13561 gtcttcgtga agaaatcaac cttcaatacc aagctcttgg tgaagttgta aaacttggtg
    13621 acctttacgg cgtagatgtt cgtaaaccag ccatgaacac taaagaagcc attcaatggg
    13681 tgaacattgc tttcatggct gtatgtcgtg ttatcaatgg tgctgcaaca tcacttggac
    13741 gtgttcctat tgtccttgat atctttgcag aacgtgacct cgctcgcggt acattcacag
    13801 aatcagaaat ccaagaattt gttgatgatt ttgtccttaa actccgtact gtaaaatttg
    13861 ctcgtactaa agcttatgat gccctttact caggtgaccc aacctttatc acaacttcaa
    13921 tggcaggtat gggagctgat ggacgtcatc gtgttaccaa aatggactat cgtttcttaa
    13981 atacacttga caatatcggt aactcaccag aaccaaactt aactgttctt tggtcagatc
    14041 aattaccata tgctttccgt cgctactgta tgtcaatgag tcataaacac tcttcaatcc
    14101 aatatgaagg tgtatcaaca atggctaaag aaggctacgg tgaaatgtca tgtatttcat
    14161 gttgtgtatc tccacttgat ccagaaaacg aagacaaacg tcacaacctt caatattttg
    14221 gtgctcgtgt taacgttatg aaagcacttc ttactggtct taacggtggt tatgatgatg
    14281 ttcataaaga ttacaaagta tttgacatcg acccaattcg tgacgaagta ctcaactttg
    14341 acactgtaaa agctaacttt gaaaaatctc ttgactggtt gactgatact tatgttgatg
    14401 cgatgaatat catccactac atgactgata aatacaacta tgaagctgta caaatggcat
    14461 tcttaccttc acatgtacgt gctaatatgg gatttggtat ctgtggtttt gcaaatactg
    14521 tcgattcatt atctgcgatt aaatacgcta ctgttaaacc aatccgtgac gaagatggtt
    14581 acatctacga ctacgaaaca gttggtgact tcccacgtta tggtgaagat gatgatcgtg
    14641 ttgactcaat cgctgaatgg ttacttgaag ccttccatgg acgccttgct aaacacaaac
    14701 tttataaaga tgctgaagca actgtttctc ttcttacaat cacatcaaac gttgcttact
    14761 ctaaacaaac tggtaactca ccggtacata aaggagtata tctcaatgaa gatggtagtg
    14821 taaacctttc taaagtagaa ttcttctcac caggtgctaa cccttctaac aaagctaaag
    14881 gtggctggtt acaaaacttg aattcacttt ctaaacttga tttcgctcat gcaaatgatg
    14941 gtatttcatt gacaactcaa gtttctccac gcgctcttgg taaaacattt gatgaacaag
    15001 ttgataactt ggtaacagtc cttgatggtt actttgaaaa cggtggtcaa cacgttaact
    15061 tgaatgtcat ggaccttaaa gatgtctatg acaagattat gaatggtgaa gatgttatcg
    15121 ttcgtatctc aggttactgt gtcaatacta aatatctaac tcctgagcaa aaaactgagt
    15181 taacacaacg tgtcttccat gaagtacttt caatggacga tgcattaact aactagtttg
    15241 tattcgatga agtaggtttg attatatcaa acctactttt atctatatta tcaatagtac
    15301 taattacttc tttatataac ctcaaggagg ggcctaatgt taaatcacaa atttcttcaa
    15361 gttctaaaat atgagggtgt cgtttcgata acaagctgga ccgaattagc tcctcacgtt
    15421 acaaacactt ggaatagcta cctgactatt acagatgacc agcgtatatt agctcctgca
    15481 gcaggaatga ctcatcttga aatgatctca ataataattc taaaattatt atgactctgg
    15541 gcagccgtga agtcgagggt cgtgatggtt atcaaggtac aggctttaga attgaaggaa
    15601 ctgctaagct attagaagca ggaagcgatt ttgaaattgt taaagaaaaa tacccttttc
    15661 tgcgtaaagt attggaagta actcccataa atgttatcca acttttataa aaaacttctc
    15721 ctagaaaatt ctaggagaag ttttttattc aaagtacgat gttatcgcct cttgtacatg
    15781 tcgtcgtttc ttaatccaaa gtggacggtc atcataggaa aaggttctgt ttgtcaaaaa
    15841 aatagctgct gcctgctttt gataatttag tgcaataaaa ggaccagtat aaccagtatg
    15901 gtttatccaa tccttatcta tatcccaagc taatgatctt gatttactca aactataatt
    15961 attccacaaa ggcttcgcaa aatcatcttt taaataatga atagaaaatc tttgtaaatc
    16021 atcaatagtt gaaaataaac ctgcagaacc agtatgcttt cctaatacct tagctttagg
    16081 atcgtgtaca ataccatcgt taataccggc tacagttgga acagcttcta ctctaggacc
    16141 aaaagaggtt tctttcatct gaaaaggagt gaaaatataa cgtttgaaaa gtttatccaa
    16201 actatcccct aatacctctt ctaacataaa accaagtaaa agaaaattaa tatcagtgta
    16261 tttaaacgat ttatcttcca aaaccttgat atgattaatg gcatctttca gctgagtagc
    16321 attcaactga tcacgatttg gaataaaagg gtcaattcca ctgctatgtg tagctaattg
    16381 ccttacagtt aacgtctgat gatgaaacgt agggtaataa taccttaatg gcttatcaat
    16441 atcaatagtc ccttgataaa ctaactttgc tagtaaagtt cccacgccga caacttttga
    16501 tacactggct agatcataga ccaatcccgc tttaactttc tcattgccat caattgtccc
    16561 caagtaatgt tcgtgccact ctccattttc ataaatagct aagcttgcac catgataaag
    16621 attatctttg atttgctcct taatgagttc taaaacggta ttagttttaa tcattctttt
    16681 gaaatccaaa tctcaatatt actagtgtca gagatgacta aaatcttttc tttagaatca
    16741 aggtaaacat caagtcccca tgattcaaaa gttgatttca agtcatttaa attagtatct
    16801 tcattaacac agcattctag tatctcaata tcccaagttt catttggagc aatttgcaaa
    16861 tcttgtccct gtgcttcttt gaaagataaa ttaattggga acttcccagc aaatacttta
    16921 tcgtaaaaag cttcagcttt cacggcatca ggtacattca aactaataga ttggaaagca
    16981 aagtttgata gacctgtagt gtcatctttt ttttctaact ctgggcgctc aattgccact
    17041 aattgtgaca aatcatcttc agcatgaagt aagaaaaggt ctccctcggg cgaaactgtt
    17101 tcataggcat aaccattttg accttgatat acttgaatgg cttgcgcacc atttgccaaa
    17161 agcttttcaa tatcttttgc atcttgagac ttaacaataa tctttgctaa cttcttagta
    17221 ccattcaccg cacgagtacg ataagttggg gattcctcaa taataaagct agcctctttg
    17281 ttctgccatg ctgagaaaac tgcgattgca ttttcttcac ttatcaattt aaatccaaga
    17341 gactcctgat aaaatgcaat attcaagtct ctattattaa cacgtaatac tgggtgttta
    17401 aatgtcaatg aatgaaataa cgtcataaat tcctccttgt tcagtctagt ctattttaga
    17461 taattttaga taatttagca agaatctttg tgctctcaat accataaagt caaattgaca
    17521 gtttcactat ttttttgtag actggatatt atgaatgaaa cactactatt acatgggatt
    17581 cagcttattc tgattatcgc catgattatc actttttatc agattgttag acatattagg
    17641 tctcaaaaaa taaatccttt caaacgattt ttcactggtt tgtggatcgg ttttgtaact
    17701 gatgctctcg atactcttgg tatcggctct tttgcaacaa cgaccacatt ttttaaacta
    17761 actaagcttg ttgaagacga tcgtaagata cccgcaacaa tgacagctgc acacgtactt
    17821 ccagttttgt tgcagtcact ctgttttatt tttgttgtaa aagtagaggc tttaacacta
    17881 ataacaatgg ctggtgcagc ttttattggg gcttttgttg gagctaaaat gactaaaaat
    17941 tggcacgctc ctactgttca acgtattcta ggaacactgc tcatcacagc tgctattatt
    18001 atgctctatc gtatgatcac taaccctggc gctggtattt cagattcagt gcatggtcta
    18061 cacggcattt ggttatttgt tggtatcgtc ttcaatttta ttattggtgt attaatgact
    18121 atgggactag gtaattatgc tcccgaactt atcttcttct ccctaatggg actgagtcct
    18181 gcagttgcta tgcctgtcat gatgctagat gcagctatga taatgacagc ttctagtaca
    18241 caatttatca agagtggtcg tgttaattgg aacggctttg ccggtctggt tactggggga
    18301 atattaggcg ttattgtcgc tgttctcttc ttaactaacc ttgatcttaa cagcttgaaa
    18361 acactggttg taggtattgt tttatttact ggagcaatgt taatacgttc ttcctttaaa
    18421 aaaactatgc ccaaaagtca tcattaaacg aaatcagcta gcaagactgg ctgattttta
    18481 tatttaaaac aaaaaaagac cggtaaaccg atctttcttt aactattaaa gatataatag
    18541 tttgaacaag gctacagcga gtatacttgc tacgattggt gctaaaactg gcacccatgc
    18601 ataccaccat tttgaatctt ctttagcttg tccaagaatt tgttttggta ataagcggtg
    18661 aacaatacgt ggaccaaggt cacgcgcagg gttcaatgca ggtccagtcg gtccacctaa
    18721 agaagcaact agagtcatta ccaagaaacc aatacctaag tgagctacag caagtgaacc
    18781 agttacgtat ggtgaaatcc ttgtcgctgc tgtcgtttgg tcataaccag cttgaacaag
    18841 tttaccaaca agttctacac caaaatagtt cttcgttaaa gccaaagctc caaagaaaag
    18901 aacaaaagaa ccaacgaatt catttaagaa accattaatg taagccgctt tacgacttga
    18961 tttctgtcca tcatctaatg cagaaatagt tgaaaatgat ccaagaacat gatttggatt
    19021 ttctgtctta acaaagtaag gttgataaac cataacgaca actaactgtc caaatattgc
    19081 tcctaaaatt tgtgcaagaa tgtattgtcc aacgtgtgcc caagggaaaa gacctgaaaa
    19141 tgccaatcca agtgtgaaag ctgggttaat atggtttcca gagacattac caaacataag
    19201 cgctggcatc ataacaccta aaccataacc aattgcaata ataatccaac ctgaattatt
    19261 tccttttgtg ccttttaagt caacgttagc tacagcccca tttcctaaga tgataagaaa
    19321 agcggttgcg ataaattctg tgatatactt cacagtccaa gtgatttcga tcaatccttg
    19381 tttcctcccc aaaaaatatt tacaacatta accattctac caagtaagct taatattgta
    19441 aagcttatca caaaataaaa ttaataaaat ttttaaccaa atatatttga taaaaaatag
    19501 agtactaggt tcggactacc tagtactcta ttttatttag tttataaggt tttcttacta
    19561 tcacgaacga ctagcatatc aactttcgaa tgacgtaaaa tgtattctga agttgagccg
    19621 atcaataggc tttcaaaagt gtttaatcct gttgcgccta ccataattaa atctgctttt
    19681 tcccttatgg ggatatcctt tgccaaaaga gtcttaggat ttccgatttc agtaattttt
    19741 ttaatctttg taaggccttt tgaatgtgct aatttctcaa aataggctag cacatctgct
    19801 gactcttgtt cttgcctatc aaaaacatag tcactaaaga ctccctcact ttggtaagcg
    19861 tgagcatcta taacatgcgt tagcagtaat tcagcatcat tacgaagagc aacatttatc
    19921 cctttttcta ccgccaattc tgactcgtaa gacccatcaa tagcaataag aatacgctca
    19981 tatttttgtg tcatagctta attcctccct tttatatagt tttagtttca acttgtctag
    20041 atgattttaa gaaatataga ataccacaaa caacaggaat aatcgctgct atccagaata
    20101 attctctaaa ggattgcaca ccagctccaa gaaaaccatc tttaacaagt cccaaaatgt
    20161 aaggtccagc acctaaccct aaatcaagac ctatcatgta agttgacatg gctgtattga
    20221 aacgatgttc ctcaacacct ttgattgatg ctgcttggcc acaagacata aaggtgccat
    20281 aaccaaaacc aatcaaagca cctgatagaa ggtaggtaac acttcccata gcactcccta
    20341 ataaagcaag tcccaaagtt aagaacagat aacttggata aagcacccac ttatctccct
    20401 tagcgtccat taatcttccc atagatggtc tggttaaagt gatgacaagt gcataaacaa
    20461 taaagaaata agctcctact gtcatcaaat taatttctgt tgtatataat ttttggaaac
    20521 ctaacacgga agcataggag atacccatca aaaatgcaat aattgtgata aaaattgctt
    20581 ttttctcaat gaaactatca atagtccatg attttgattt agctaactgt tctggattta
    20641 aagtaatatt tttgactggg aaaacaaatg ctcccaacac tacaatcgca attaaaatac
    20701 tacataatac aataaccatt ttaaagttaa tatgaaggtt gtctagcata aatgttccta
    20761 caaaaggacc aatagctgcg gctaaacttg ttgatagacc gtaaaagtta atcccctcac
    20821 ctcttttatc agctggtata taggctgtta caatagtatt agttgctgtt gacacgacgc
    20881 cataaccaaa accatttagg aaacgaacta aatacattac tccgatactt ggcatataaa
    20941 aataagctaa agttgttagt aagtaaaaaa tagcccctcc acgtaaaact aacttacgtc
    21001 ctagaacttc taattgctta ccaaatataa gacgagcaat caaagtccct acaatataaa
    21061 tccccgttgc taatcctgct tggctagtgc taacacctaa ctctttagtc gcaataaaag
    21121 ctatgataac ggtaaacaaa taatagacca tataaacaat aaagtttaaa a
//
LOCUS       DATROD010000022        20055 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.022, whole genome shotgun sequence.
ACCESSION   DATROD010000022 DATROD010000000
VERSION     DATROD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20055
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.022"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(82..2664)
                     /locus_tag="VBC74_000825"
     CDS             complement(82..2664)
                     /locus_tag="VBC74_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO6941790.1"
                     /translation="MNRKKTVIISALSVALFGTGVGAYQLGSYNAQKSDNSVSYVKTD
                     KSDSKAQATAANKTPDQISKEEGISAEQIVVKITDDGYVTSHGDHYHYYNGKVPYDAI
                     ISEELIMKDPSYVFNKADVINEVKDGYIIKVNGKYYLYLKEGSKRTNVRTKEQIQKQR
                     EEWSKGGSKGESGKHSSAKTQALSASVREAKASGRYTTDDGYVFSPTDVIDDMGDAFL
                     VPHGDHFHYIPKADLSPSELSAAQAYWNRKTGRSGNSSKPSNSSSYIHASAPSGNVST
                     GRHANAPISIPRVTHANHWSKPAGNHATAPKHHAPTTKPIKKDSALDKMLKRLYAQPL
                     SARHVESDGLVYDPAQVNAFTAIGVSIPHGNHFHFIHYKDMSPLELEATRMVAEHRGH
                     HIDALGKKDSTEKPKHISHEPNKEPHTEEEHHAVTPKDQRKGKPNSQIVYSAQEIEEA
                     KKAGKYTTSDGYIFDAKDIKKDTGTGYVIPHMTHEHWVPKKDLSESELKAAQEFLSGK
                     SEANQDKPKTGKTAQEIYEAIEPKAIVKPEDLLFGIAQATDYKNGTFVIPHKDHYHYV
                     ELKWFDEEKDLLADSDKTYSLEDYLATAKYYMMHPEKRPKVEGWGKDAEIYKEKDSNK
                     ADKPSPAPTDNKSTSNSSDKNLSAAEVFKQAKPEKIVPLDKIAAHMAYAVGFEDDQLI
                     VPHHDHYHNVPMAWFDKGGLWKAPEGYTLQQLFSTIKYYMEHPNELPKEKGWGHDSDH
                     NKGSNKDNKAKNYAPDEEPEDSGKVTHNYGFYDVNKGSDEEEPEKQEDESELDEYELG
                     MAQNAKKYGMDRQSFEKQLIQLSNKYSVSFESFNYINGNQVQVTKKDGSKVLVDIKTL
                     AEVK"
     gene            complement(2691..3614)
                     /locus_tag="VBC74_000826"
     CDS             complement(2691..3614)
                     /locus_tag="VBC74_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEO6941791.1"
                     /translation="MKRIRKSLIFVLGIVTLICLCACTKQSQQKNGLSVVTSFYPVYS
                     ITKAVSGDLNDIKMIRSQSGIHGFEPSSSDVAAIYDADLFLYHSHTLEAWARRLEPSL
                     HHSKVSVIEASKGMTLDKVHGLEDVEAEKGVDESTLYDPHTWNDPVKVSEEAQLIATQ
                     LAKKDPKNAKVYQKNADQFSDKAMAIAEKYKPKFKAAKSKYFVTSHTAFSYLAKRYGL
                     TQLGIAGVSTEQEPSAKKLAEIQEFVKTYKVKTIFVEEGVSPKLAQAVASATRVKIAS
                     LSPLEAVPKNNKDYLENLETNLKVLVKSLNQ"
     gene            complement(3869..4312)
                     /locus_tag="VBC74_000827"
     CDS             complement(3869..4312)
                     /locus_tag="VBC74_000827"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEO6941792.1"
                     /translation="MKIIIQRVSQASVSIEDDVVGSIEKGLVLLVGIAPEDTTEDIAY
                     AVRKITSMRIFSDDEGKMNLSIQDIKGSVLSISQFTLFADTKKGNRPAFTGAADPVKA
                     NQFYDIFNQELANHVSVETGQFGADMQVSLINDGPVTIVLDTKNK"
     gene            complement(4322..6538)
                     /locus_tag="VBC74_000828"
     CDS             complement(4322..6538)
                     /locus_tag="VBC74_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEO6941793.1"
                     /translation="MVKEINLTGEEVVAITSQYMSETDVAFVKFALNYATAAHYYQAR
                     KSGEPYIIHPIQVAGILADLHLDAVTVACGFLHDVVEDTEITLDEIETDFGKDVRDII
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLSFRYLNETEFYKISHMMSEKRREREELV
                     DIIVDKIRSYTEEQGLYGDIYGRPKHIYSIYRKMRDKKKRFDQIYDLIAIRCIMETAS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKEMHQV
                     AEFGVAAHWAYKKGITSKVNQAEQSVGMGWIQELVELQDESKDAKDFVDSVKEDIFTE
                     RIYVFTPNGAVQELPRESGPIDFAYAIHTQVGEKATGAKVNGRMVPLTAKLKTGDVVE
                     IITNPNSFGPSRDWIKIVKTNKARNKIRQFFKNQDKETSINKGRELLVDYFQEQGYVP
                     NKYLDKKHIEEILPRVSVKSEEALYAAVGFGDLSPISIFNKLTEKERREEERAKAKAE
                     ADELINGGEIKTDKRDVLKVKSENGVIIQGASGLLMRIAKCCNPVPGDLIEGYITKGR
                     GVAIHRSDCQNLKSQENYEQRLIDVEWDDDGSKKEYMAEIDIYGLNRSGLLNDVLQTL
                     SNATKLVSTVNAQPTKDMKFANIHVSFGISNLAQLTTVVDKIKIIPDVYSVKRTNG"
     gene            6798..9200
                     /locus_tag="VBC74_000829"
     CDS             6798..9200
                     /locus_tag="VBC74_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase/3'-nucleotidase"
                     /protein_id="HEO6941794.1"
                     /translation="MSKHYFSKSIFALTVLTATTTSGLAVQAEDIVTTPSSTATKVES
                     TTPTSTIAEEKSNVTSTPTAITDASTATSTNTTANNQNPQPVATETTTGDLKPIEGEK
                     VDLRIMSTTDLHTNLVNYDYYQDKESQKIGLAKTAVLIEEAKKENPNTVLVDNGDVIQ
                     GTPLGTYKAIVKPVAENEEHPMYQAMNALGYDASTLGNHEFNYGLDYLKKIIATANLP
                     ILNANVLDFKTHQPVFKTYDIITKTFKDSTGRAVALNIGITGIVPPQILNWDKANLEG
                     KVIVKDSVKAIEEIVPTMRAKGADVILVLSHSGIGDDRYEEGEENVGYQIASIKGVDA
                     VVTGHSHAEFPSGNGTGFYEKYTGVDGINGKINGTPVTMAGKYGDHLGIIDLGLSYTN
                     GKWQVSESSAKIRKIDMNSTTADERIIALAKEAHDGTINYVRQQVGTTTAPITSYFAL
                     VKDDPSVQIVNNAQRWYVANELKGTPEANLPLLSAAAPFKAGTRGDATAYTDIPAGPV
                     AIKNVADLYLYDNVTALLKVTGADLREWLEMSAGQFNQIDPNNKAPQNIINTEYRTYN
                     FDVIDGLTYKFDITQPNKYNKDGKVVNSQASRVRDLMYNGKPVADKQEFMIVTNNYRA
                     SGTFPGAKNATMNRLLNLENRQTIINYIISEKTINPTADNNWGFTESIKGLDLRFQTA
                     DKAKNLVTNSKDIQYIASSTKDEGFGDYRFVYTEQEKVDLTEPSHQEKLSQKEKQKIH
                     HMVATAYTKAKASPNIAGTIYTQQKEKTLPKTGQEKQSYLAFFGVAILGLVGNLLKKK
                     QI"
     gene            9357..9812
                     /gene="nrdI"
                     /locus_tag="VBC74_000830"
     CDS             9357..9812
                     /gene="nrdI"
                     /locus_tag="VBC74_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO6941795.1"
                     /translation="MPKLTLVYISLSGNTQSFVKRLSEQLSTWHNCQVSTINIKEQNH
                     QTFPVDQPFVALLPTYLEGGNGIDNGDQEILTIPLGDFIAAHNNVKNCIGIVGSGNRN
                     FNNQYCLTAKQYSERFGFPMLGDFELRGTSSDVERIANIIVETLSNFKA"
     gene            9823..10860
                     /locus_tag="VBC74_000831"
     CDS             9823..10860
                     /locus_tag="VBC74_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEO6941796.1"
                     /translation="METTLNYIKTLTSIPSPTGFTQTIMTYIIKELEAFGYSPIRTNK
                     GGVMVSLKGKNNTKHRMITAHLDTLGAMVRAIKPDGRLKIDLIGGYTYNAIEGENCTI
                     HLSKNGKEISGTALIHQTSVHVYKDAGTAERNQTNMEIRLDEKVTTADETRALGIQVG
                     DFISFDPRTIITGSGFIKSRYLDDKVSAGILMELLSVYKKEDIQLPYTTHFYFSAFEE
                     LGHGANSSIPNETVEYLAVDMGAMGDDQETDEYTVSICVKDASGSYHYELRQHLVSLA
                     ENNNIPYKLDIYPYYGSDASAAMRAGAEVKHALLGAGIESSHSYERTHIDSIQATELL
                     VDAYLKSNMVN"
     gene            10860..11357
                     /locus_tag="VBC74_000832"
     CDS             10860..11357
                     /locus_tag="VBC74_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO6941797.1"
                     /translation="MVATLIIIRGNSASGKSTIAKQLQAELGEDTLLLSQDYLRREML
                     GTKDGENTTTIPLLINLLNYGYHNCSYIILEGILRSDWYTPVWKHILKHNPNNTYAYY
                     YDLSFQETVKRHSTRLKSLEFGEDSLARWWLEKDFLKEIPEKILTKSMSLEDVKKLIM
                     TDVLP"
     gene            complement(11579..12616)
                     /locus_tag="VBC74_000833"
     CDS             complement(11579..12616)
                     /locus_tag="VBC74_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO6941798.1"
                     /translation="MKEKQSKRLIYILLIVPIIFISVFTYSISQPSKLLPPKELVILS
                     PNSQAILTGTIPAFEEKYGIKVKLIQGGTGQLIDRLSKEGKQLKADIFFGGNYTQFES
                     HKALFESYVSKNVHTVIPDYIHPSDTATPYTINGSVLIVNNELAKGLTIKSYEDLLQP
                     SLKGKIAFADPNTSSSAFSQLTNILLAKGGYTNPKAWNYVKKLQHNINAIKSSSSSEV
                     YQSVAEGKMIVGLTYEDPSVNLQKSGANVSIVYPTEGTVFVPSSVAIIKNAPSMKEAK
                     LFINFMLSLDVQNAFGQSTSNRPIRKDAQTSNGMKALKDIATLKEDYRYVTKHKSQIL
                     KTYNRIRRNAD"
     gene            complement(12628..13401)
                     /locus_tag="VBC74_000834"
     CDS             complement(12628..13401)
                     /locus_tag="VBC74_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO6941799.1"
                     /translation="MYRLLIVEDEHLIRKWLRYAIDYQSLNILVVGEAKDGKEGAQLI
                     QEEQPDIVLSDINMPIMTAFDMFEATKGQSYAKIILSGYADFPNAQSAIHYGVLEFLT
                     KPLEKQALIDCLKTIMARIEEHKEKHLQEHTELYLPLPQANDQVPEVIKDMLAWIHSH
                     FHGKIVISQLAHDLGYSESYLYTVTKKHLHITLSDYINQYRINQAIQLMFREPDLMVY
                     QIAEAVGIYDYRYFDRVFKKYLGQTVKAFKEEHIFKQMD"
     gene            complement(13405..15054)
                     /locus_tag="VBC74_000835"
     CDS             complement(13405..15054)
                     /locus_tag="VBC74_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6941800.1"
                     /translation="MRGYRMEERFKKRLQDDISKHFSRQSLILSLLLIALFVLFSLAP
                     QQIGLYKDVNSVSYSYKQLIQKHDTLLDDLGKNSLKPFVSGHLGSADLSKQYYHLRNH
                     LQSQTELLVFSPNQELLFASNSHLGNFFSKSIYISEVLDKAKINQRLLKIIVDSEGGH
                     YLALIKPIIVNKKVSGYAFLLMNGKDFLLPTKAINSDLIIADQLNNSFTFTNRDFISS
                     SLDKVDSQFLTRYFSFHDHRAFVVRKVALQDNILLYMYRPLIPVTLVVLFSLVSSVII
                     FVILRRKSRVLADRIAVKNSGAINQMVLDMDAISRQEKSSIELDSQDEFQYLSVQINQ
                     MVSRLKDLHEKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSQLTERIV
                     IQLTKLLRYSLSGSTEAAVLKDDLAIIESYLLINQVRFEELTYTISVSPELEHMRVPK
                     LFLLPLIENAIKYGLKERHDVAINIDIWQDSDGIWFTVSNNGSGISLARQKAIRTMLR
                     SNHSHHGLINSYRRLQYQFSTVLLEFTKTDDAFRVSYIVKE"
     gene            complement(15192..16019)
                     /locus_tag="VBC74_000836"
     CDS             complement(15192..16019)
                     /locus_tag="VBC74_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO6941801.1"
                     /translation="MKSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASTGSAMGAVVFLLLYNIPAFLIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQSYLDQLFVGVVPLLVTILAFWLLRKKVNINWIMFGIMVLGIVLGLLGIC"
     gene            complement(16016..16825)
                     /locus_tag="VBC74_000837"
     CDS             complement(16016..16825)
                     /locus_tag="VBC74_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEO6941802.1"
                     /translation="MIMPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGMLLGDI
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISNIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNSFVHHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDIELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            complement(16829..17332)
                     /locus_tag="VBC74_000838"
     CDS             complement(16829..17332)
                     /locus_tag="VBC74_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6941803.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEESIALFKDPRATDKRIFVIVNSVKDACTIAKNITDLEAVN
                     VANVGRFDKSDPATKVKLTSSLLLNPEELEAAKELASLPDLDVFNQVLPSNTKVNLSQ
                     LVKQSEV"
     gene            complement(17350..17775)
                     /locus_tag="VBC74_000839"
     CDS             complement(17350..17775)
                     /locus_tag="VBC74_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEO6941804.1"
                     /translation="MKRKFLIGSHGKLASGLQSSIDILTGKGQEIQTIDAYIDDSDYT
                     KSIVEFIDEIAPDEQGLIFTDLLGGSVNQKMATAVMNSGKNNVFLITNSNLATLLSLL
                     FLKPEEELTKEEIVTVINESQVQLVDLSFKACSEDDFFD"
     gene            complement(18022..19056)
                     /locus_tag="VBC74_000840"
     CDS             complement(18022..19056)
                     /locus_tag="VBC74_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEO6941805.1"
                     /translation="MDNKQHNTSMISLLQYLFSILVILVHSGRLFSQDVIHFTFKSFL
                     GRMAVPYFLICTAFFLRGRIQQGLCNHSYFRKLIKKYSMWTIIYLPYGYFFFESLNIA
                     KIYLLPGFIVAFLYLGMSHTLWYIPAVILGWFIIQGLLKYVGTRGTFITVVVLYCIGA
                     VETYSVFVQSTKFYPLMSTYMSIFQTTRNGLFYTPVYLLAGYLLYDYFNTDLFTKSRG
                     LKYILFLLLLALENVLIYFNQGLDKNFFLLAPLCAVFLFNWSIRTSLFKGYRLSPLKQ
                     LSVYYFFLHPLFVGIVSYCLKSTSLVAHHQGKVIFVVTLALTHACALLIIKVVNKIPY
                     QKIIPLSLSK"
     gene            19108..19290
                     /locus_tag="VBC74_000841"
     CDS             19108..19290
                     /locus_tag="VBC74_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941806.1"
                     /translation="MIPISSEEYLNYPQKSLKRLKWYLIIIVNTLSTILGNKKADFAK
                     ENQLRIISCSDNQWEL"
     gene            complement(19285..>20055)
                     /locus_tag="VBC74_000842"
     CDS             complement(19285..>20055)
                     /locus_tag="VBC74_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941807.1"
                     /translation="KDQKQLGSALPLKQLKLGNFAPWNQLFEVSNSTYILIVLFTLFL
                     SAPLFSGDRAKKINPILLTTRYGRGQLTWAKLASTMTIGISTFILAYALILTVFADYF
                     GFSGWETSVQLNLYWTSPLANIMGFPVPMTISTAFVHIIVLQFVGLLFIASLNFFILS
                     ITNSPLTSFAVSTLAFFAPSFLMAMFNKGVINKLLTIFSVSTTDTSALLLKLSRNGSH
                     GFFFDSFSANEICLISLRMLIAVLLIAYTYRLMRRRSL"
BASE COUNT         6487 a         3884 c         3280 g         6404 t
ORIGIN      
        1 ctagaaatca taactaaaaa acaaacaagt gcggtgactt gtttgttttt agttgtcagc
       61 taagtttatt caactctttt attatttcac ttctgctagt gttttaatat caacaagcac
      121 tttagagcca tcttttttag taacttgaac ttgattacca ttaatataat taaagctttc
      181 aaaacttaca ctatatttat ttgataattg gatgagttgc ttttcaaaag attgtctatc
      241 cataccatat ttcttagcgt tttgtgccat tcctagttca tattcatcta gctctgattc
      301 atcttcttgt ttttctggtt cttcttcgtc tgaaccttta ttaacatcat aaaaaccata
      361 gttgtgagtt actttccctg aatcttcagg ttcttcatct ggagcataat ttttggcttt
      421 attgtcttta tttgagcctt tgttatgatc actgtcgtgt ccccaaccct tttcttttgg
      481 taattcatta ggatgttcca tgtagtattt aattgttgag aagagttgtt gtaatgtata
      541 gccttctggt gctttccata aaccaccctt gtcaaaccat gccataggaa cattatgata
      601 atgatcatga tgaggaacaa tcaattgatc atcttcaaat ccaactgcat atgccatgtg
      661 agcagcaatt ttatcaagcg gtacaatttt ttctggtttt gcttgtttga atacttctgc
      721 agcacttaag tttttgtcac tagaatttga tgttgattta ttatcagttg gtgcaggact
      781 tggtttatct gctttattag agtccttttc cttataaatt tcagcatctt taccccatcc
      841 ttcaacttta ggacgttttt ctgggtgcat catgtaatat ttagccgtag ctaaatagtc
      901 ttctaaagaa tatgtcttat ctgaatcagc taaaagatct ttttcttcat caaaccattt
      961 taattccaca taatggtaat gatctttatg aggaattaca aatgtaccat tcttatagtc
     1021 tgtcgcttgt gcaattccaa ataataaatc ttcaggttta acaattgctt ttggttcaat
     1081 tgcctcatag atttcttgag ctgttttacc tgtttttggt ttgtcttgat ttgcttcaga
     1141 ttttcctgaa agaaattctt gagctgcttt taattccgac tctgataaat ctttctttgg
     1201 tacccaatgc tcatgtgtca tatgtggaat gacataacct gtacctgtat cttttttaat
     1261 atctttagca tcaaaaatgt aaccatcaga tgttgtgtat ttaccagctt tttttgcctc
     1321 ttcaatttct tgagcactgt agacaatctg gctatttggt ttgcctttac gttggtcttt
     1381 cggtgttact gcatggtgtt cttcctctgt gtgaggttcc ttattaggtt catgagaaat
     1441 atgctttggt ttctctgtag aatctttttt ccctaatgca tcaatatgat gtcctctatg
     1501 ctctgccacc atccttgttg cttctaactc taatggagac atatccttat agtgaataaa
     1561 gtggaaatga ttaccgtgcg gaatagaaac accgattgct gtgaaagcat taacttgtgc
     1621 tgggtcataa accaaaccat cactttctac gtgacgagca gataacggtt gagcatataa
     1681 acgtttcaac attttgtcta aagcagaatc ttttttgatt ggtttggttg taggtgcatg
     1741 gtgttttggt gccgtagcat gattaccagc tggttttgac cagtgatttg cgtgagtgac
     1801 tcttggaata gaaatagggg cattagcatg tcttcctgtt gatacattac cgcttggtgc
     1861 acttgcatga atgtatgaac ttgaatttga tggttttgag ctattacctg atcggccagt
     1921 cttgcggttc caatatgcct gtgcggctga taactctgat ggagataaat cagctttcgg
     1981 gatataatgg aaatgatcgc catgtggtac taaaaaggca tctcccatgt catcaataac
     2041 atctgttgga ctaaaaacat aaccatcatc tgtagtatag cgaccgctag ctttcgcttc
     2101 acgtacgctg gctgataaag cttgagtttt tgcactacta tgctttcctg attctccctt
     2161 agaaccaccc ttagaccatt cttcgcgttg cttttggatt tgttcctttg tacgaacatt
     2221 tgttcgtttg ctaccttctt taagatagag gtaatactta ccattaactt tgataatata
     2281 accatctttg acttcattaa tgacatcagc tttattaaag acatagctag ggtctttcat
     2341 aatcaattct tcactaatga tggcatcata tggcactttc ccattgtaat aatggtaatg
     2401 gtctccatga gaagttacat aaccatcatc agtaatttta acaacgattt gttctgcaga
     2461 tatgccttct tctttactga tttggtcggg cgttttattt gcagctgttg cttgtgcttt
     2521 gctatcagat ttatctgttt tcacataaga aacactattg tctgattttt gggcattata
     2581 gctacctaat tggtaagcac ctacaccagt accaaataga gctactgata aagcactaat
     2641 aataactgtt tttttacgat tcatcctgcg tcctttcttt atataactac ctattgattt
     2701 aacgatttga caagtacctt aagattagtt tccaaatttt ctaagtaatc tttattgttt
     2761 ttgggaactg cttctaaagg acttaaactt gcaattttaa ctcgagtagc tgaagctact
     2821 gcttgagcta atttaggtga gactccttct tcaacaaaaa tagtcttaac cttatatgtt
     2881 ttcacaaact cctgaatttc ggctaatttt ttagcactag gttcttgctc ggttgagaca
     2941 cctgcaatac ctaactgagt caatccgtat cgcttagcta agtatgagaa tgctgtatgt
     3001 gaagtcacaa agtatttaga ctttgcagct ttaaattttg gcttatactt ctctgcaata
     3061 gccattgcct tgtcactaaa ttgatcagca tttttttgat aaaccttagc gtttttagga
     3121 tcctttttag ctagttgtgt agcgatgagt tgtgcttcct cagatacttt tacagggtca
     3181 ttccaagtgt gagggtcata caaggttgac tcatctactc ctttttctgc ctctacatct
     3241 tctaagccat gaactttatc caaagtcata ccttttgaag cttcaattac agatacttta
     3301 gagtgatgca aactaggttc caaacgtctc gcccaagctt ctagtgtgtg cgaatgataa
     3361 agaaatagat cagcatcata aatggcagca acatcacttg atgagggttc aaaaccatga
     3421 atgcctgact gtgatcgaat cattttaata tcattcaaat caccagaaac tgcttttgta
     3481 atggaatata ctggataaaa gctagtcacg accgacaagc catttttttg ctggctctgt
     3541 ttagtacaag cacataagca aattagggta actattccga gaacaaaaat aaggcttttc
     3601 cgtatccttt tcattaaacc tcctttaaaa taaaaagtac ttaaccagtt aacattttag
     3661 agtattctta ttttagtgtc aacgaaaaac cataaatcta atatttttct atttttaata
     3721 ttgttaagtg cacttaattt catatcaaca acctttattc atgcctttcc aagctacagt
     3781 gaatcttttt ataaacatga atgggttaag gagtataatt agaaccacaa taaaaagagc
     3841 caagcatttt gcccaactct ttgatttttt atttattttt agtatctaaa acgatagtta
     3901 ctggtccatc attaatcaga ctgacttgca tatcagctcc aaattgacct gtttctaccg
     3961 aaacatgatt tgctaattct tgattaaaga tatcataaaa ctgattcgct ttaacaggat
     4021 cagctgcccc agtaaaagct ggacgatttc cttttttggt atctgcaaag agtgtaaatt
     4081 gtgagataga taagacgctt cctttaatgt cttgaattga aaggttcatt ttcccctcat
     4141 catcagaaaa aatacgcatg ctagtaattt tacgaactgc ataagcaata tcttctgttg
     4201 tatcttctgg agcaatccca actagaagta ctaaaccctt ctcaatagat ccgactacat
     4261 catcttcaat agaaacactt gcttgactaa cacgttgaat aatgattttc attttgctct
     4321 cctaaccatt cgtacgttta acagaataaa catctgggat aattttgatt ttatcaacca
     4381 cagttgtcaa ttgtgctaaa tttgaaattc caaagctaac atgaatatta gcaaatttca
     4441 tatcttttgt tggttgtgca tttactgtcg atactagctt tgttgcattt gataaggttt
     4501 gaagaacatc attaaggagg ccactacgat tcaaaccata aatatcgatt tcagccatgt
     4561 actctttttt agatccatca tcatcccact caacatcaat caaacgttgt tcgtagttct
     4621 cctgactctt taaattttga caatctgaac gatggatagc cacgcctcta cctttagtta
     4681 tatagccttc aatgagatct ccaggaacgg gattacaaca ttttgcaata cgcattaata
     4741 aacctgaagc tccttgaatg ataacaccat tttcactctt aactttgaga acatcacgct
     4801 tatcggtttt gatttcgcca ccgttaataa gttcgtctgc ttccgcttta gccttagcac
     4861 gttcctcctc acggcgttct ttttccgtca gtttattaaa aatactaatc ggactcaaat
     4921 caccaaaacc tacggctgca tacaaagctt cctcactttt aacactgaca cgtggaagga
     4981 tttcttcaat atgtttctta tctaaatatt tgttaggcac ataaccttgt tcttggaaat
     5041 aatcaaccag taattctcta cctttattaa tagaagtttc tttatcttgg tttttgaaga
     5101 actgacgaat tttattgcga gctttattgg tcttaacaat cttaatccag tcacgacttg
     5161 gtccaaatga attagggttt gtgataatct caacaacatc tcctgttttt aacttagcag
     5221 tcaatggaac catacgtcca tttactttag cgccagtagc tttttctcca acttgcgtgt
     5281 gaatagcata agcaaaatca attggtccag attcccttgg caattcctga acagcaccat
     5341 ttggggtaaa aacatagatg cgttctgtaa agatatcttc cttaacagag tcaacaaaat
     5401 ccttggcgtc ttttgattca tcttgaagct caactaattc ttgaatccat cccataccaa
     5461 ccgattgctc tgcttgatta accttactgg tgattccttt tttataggcc caatgggctg
     5521 caacaccaaa ctcggcaact tgatgcattt ctttagttcg aatctgaatt tcaattggcc
     5581 ctttaggtcc ataaaccgtc gtgtgaatag attgataacc attggccttt ggtgccgcaa
     5641 tataatcttt aaaccttcct ggcatcggac gccataactc atggatatac cccaccatgg
     5701 cataaacatc actagcagtt tccataatac agcgtatcgc aattaaatca taaatctgat
     5761 caaagcgttt ctttttatca cgcatttttc tgtaaataga ataaatgtgt tttggtctac
     5821 cataaatatc accatataaa ccctgttcct cagtatagga tctgatttta tcgacaatta
     5881 tatcaaccaa ctcttcgcgt tcacgacgtt tttcactcat catatgagaa atcttataaa
     5941 attccgtctc attcaagtaa cgaaaagata aatcttctaa ttcccacttg atacgactaa
     6001 tccccaaacg atgcgctaaa ggagcgtata tttccatagt ctcacgtgaa atacgctctt
     6061 gtttatcttt tctgaggtgt ttaagggttc tcatattatg taagcgatca gcgagcttaa
     6121 ccaaaataac acggatatcc ttagacatag ccattaacat tttacggtga ttttctgcta
     6181 actgttcttc atgagatttg tactccactt tacccaattt tgttacaccg tcaataatat
     6241 cgcgaacatc cttgccaaaa tcagtttcaa tctcatcaag ggtaatttcc gtatcttcta
     6301 caacatcatg taaaaaacca caagctaccg taacggcatc aaggtgtaaa tctgcaagaa
     6361 tacctgctac ttgaattgga tgaataatgt agggctcgcc cgactttctt gcttgataat
     6421 aatgagctgc ggttgcgtaa tttaaggcaa attttacaaa agcaacatcc gtctcactca
     6481 tgtactggga tgtgattgca acaacctctt ctcctgttaa attaatttct ttgaccatag
     6541 ttatcctctt taatcatagt gatagattgt acctatttta tcacttttta acctaaaaat
     6601 aaaagaagtt agaggctcaa aaagaattaa ataccaagta aatgtgtcta accaagttat
     6661 cctttataaa taaacgattg tttttagtgt aattactaaa aataattcgt aaatgagatt
     6721 gattaaacgt tttcaataag ctataatagt ttttagatta ggataatata catgtaatgt
     6781 tttaatggag gagatttatg tcaaaacatt atttttcaaa aagcatattc gctttaactg
     6841 tcttaacagc tacaacaaca agtggtttag ctgttcaagc agaagacatt gttacaacac
     6901 caagttcaac cgctacaaag gttgaatcaa ctacccccac tagcacaatt gcagaggaaa
     6961 agtcaaacgt tacttctact ccaactgcaa taactgatgc ttcaacagcc acaagcacaa
     7021 ataccactgc caataatcaa aatccacagc ctgttgccac agaaaccaca actggtgatc
     7081 tcaaacctat tgaaggtgaa aaagttgacc ttcgcatcat gtcaaccaca gacctacaca
     7141 ccaatttagt taactatgat tattatcaag ataaagaatc tcaaaaaata gggttagcaa
     7201 aaactgctgt ccttatcgaa gaagcaaaaa aagaaaatcc taataccgtc ttagttgata
     7261 acggagatgt cattcaaggg acacctttag gaacttataa agcaatcgtt aaacctgtcg
     7321 cagaaaatga agaacatcct atgtaccaag ccatgaatgc tcttggttat gatgcatcaa
     7381 cactaggtaa tcatgaattt aattacggtt tagattacct caaaaaaatc attgctacag
     7441 ccaaccttcc aatcctcaac gcaaatgttt tggatttcaa aacacatcaa ccagttttta
     7501 aaacttatga catcattact aaaaccttca aagattcaac aggaagagct gttgcactaa
     7561 atattggaat tacaggaatt gtacctcctc aaatcttaaa ctgggacaag gctaacttag
     7621 agggaaaggt tattgtcaaa gattcagtta aagcaatcga agaaatcgtt cctactatgc
     7681 gtgctaaagg agcagatgtc attctcgttt tatcacactc tggcattgga gatgatcgat
     7741 atgaagaagg tgaagaaaac gttggctatc aaattgccag catcaaggga gtggatgccg
     7801 ttgttacggg acactcacac gctgaatttc catcaggtaa cggtactggc ttctatgaaa
     7861 aatacactgg agttgatggt atcaatggaa aaataaatgg aacacctgtt acaatggcag
     7921 gcaagtacgg ggatcacctt ggtattattg atttaggact tagttatact aatggaaaat
     7981 ggcaagtctc cgaaagcagt gctaaaatcc gtaaaattga tatgaactca acaactgctg
     8041 acgagcgtat cattgcattg gctaaggaag cacacgatgg cactatcaac tatgttcgcc
     8101 aacaagtagg tacaacaact gcgccaatta caagttactt tgcactagtt aaagatgacc
     8161 cttcagttca aattgttaac aacgctcaac gttggtacgt tgcaaatgaa ttaaaaggaa
     8221 cacctgaggc aaatttacca cttctatctg ctgcagcacc atttaaagcc ggaacacgtg
     8281 gcgatgctac cgcatatact gatattccag ctggacctgt tgcaattaaa aacgtagctg
     8341 atctttacct ttatgataat gttactgcac ttttaaaagt gaccggagca gacttacgtg
     8401 aatggttaga aatgtccgct ggtcaattta accaaattga ccctaacaat aaagcaccgc
     8461 aaaatattat caataccgaa tatcgtacct ataattttga cgttattgat ggcttgactt
     8521 ataaatttga tattacacaa cctaataaat acaacaaaga tggaaaagtt gttaatagtc
     8581 aagctagtcg agtacgcgat ttaatgtaca atggtaagcc tgttgcagac aaacaagagt
     8641 ttatgattgt gaccaataac taccgtgcta gtggtacctt ccctggggct aaaaatgcca
     8701 caatgaaccg tttgctgaac ttagaaaatc gccaaactat tataaactat attatttcag
     8761 agaaaaccat caatcctaca gctgataata actggggctt cacagagtct attaaaggcc
     8821 ttgatttacg attccaaaca gccgataaag ctaaaaatct tgttactaat agcaaggata
     8881 ttcagtatat cgctagttct accaaagatg aaggatttgg tgattatcga tttgtctata
     8941 ctgaacaaga aaaagtagac cttactgagc catcacatca agaaaagtta agccaaaaag
     9001 agaaacaaaa aattcaccac atggttgcaa ctgcttatac aaaagcaaaa gcttctccaa
     9061 atatcgcagg cactatctat acacaacaga aagaaaaaac attacctaaa actggacaag
     9121 aaaaacaatc ttatctggct ttctttggcg tcgcaatact tggactcgtg ggaaatttac
     9181 ttaaaaagaa acaaatatag tcatcactaa aaactgaggc caatcaacct cagtttttag
     9241 tctcaacaat agattttctc tctaaacagt cagatattga gataccactc tagcttgttt
     9301 atctgacatg attttgctaa actgataaca agaaatttag atttaaggag tactaaatgc
     9361 ctaaattaac cctagtctat atcagtctta gcggcaatac tcaaagcttt gtcaaacgtc
     9421 ttagtgaaca actgtcaact tggcataact gtcaggtttc gactattaat atcaaggaac
     9481 aaaatcatca aacctttcct gttgaccaac ctttcgtagc actattaccc acttacctcg
     9541 agggaggaaa tggcattgac aatggcgatc aagaaattct taccattcca cttggtgatt
     9601 ttatagctgc acacaataat gtcaaaaatt gtatcggaat tgttggctct ggaaatagaa
     9661 attttaacaa tcagtactgt ttaactgcaa aacaatactc tgaacggttt ggatttccta
     9721 tgttaggaga ctttgaattg cgaggaactt ctagcgacgt tgaacgtatc gcaaatatta
     9781 tcgttgaaac attatcgaac tttaaagcct aaaggaaata gtatggaaac aacattaaac
     9841 tacattaaaa ccctgacatc aatcccttca ccaacagggt ttacccaaac aatcatgacc
     9901 tatatcatca aagaattgga agcgtttggc tactcaccaa ttcgcacaaa caagggaggc
     9961 gtcatggttt ctctaaaagg aaaaaataat actaaacatc gcatgataac tgctcacctt
    10021 gatacacttg gtgctatggt tagggccatc aaaccagatg gtcggttaaa aattgacctt
    10081 attggcggat acacatacaa tgccattgaa ggagaaaact gtactataca cctctcaaaa
    10141 aatggtaaag aaatttctgg aactgctctt attcatcaaa ctagtgtcca tgtttacaaa
    10201 gatgctggaa ctgctgaacg taatcaaaca aatatggaaa ttcgtttaga tgagaaagta
    10261 acaactgctg acgaaacacg tgctttaggc atccaggtcg gtgatttcat ttcatttgat
    10321 ccgcgtacaa tcataacagg cagcggcttt attaaatcac gttacctaga tgacaaggta
    10381 tccgctggta tcctaatgga acttctttct gtttacaaga aagaagacat tcaacttcct
    10441 tatactactc atttctactt tagtgccttt gaagagctag gacatggagc aaattcaagc
    10501 atcccaaatg aaactgtaga atatctagca gttgatatgg gagctatggg agacgatcaa
    10561 gaaactgacg aatatactgt ctctatctgt gttaaagatg cttctggttc ttatcattat
    10621 gaattacgtc aacatcttgt ttctctagct gaaaacaata atattcctta taaacttgat
    10681 atttatcctt attatggtag tgacgcctcc gctgccatgc gtgctggtgc ggaagttaaa
    10741 cacgcgctac ttggtgcagg tattgaatct agtcattctt atgaacgtac ccatatcgat
    10801 tctattcaag caactgaact cttagtggat gcctatctca aaagcaatat ggtgaactaa
    10861 tggtagccac gctcattatt atcaggggaa attcagctag cggaaagtct acaatagcta
    10921 aacaacttca agccgaatta ggggaagata cccttctctt atctcaggat taccttcgta
    10981 gagagatgtt aggaactaag gatggtgaaa atactactac cattcccctt ttgatcaatt
    11041 tactaaatta tggttaccat aactgttcct acatcatcct tgaaggcata cttcgttctg
    11101 attggtatac gcctgtttgg aaacatattt taaaacataa ccccaacaat acttatgctt
    11161 attactatga tttaagcttt caagaaactg ttaaaagaca ttcaactcga cttaaatctt
    11221 tagaatttgg agaagattct cttgcaaggt ggtggcttga gaaagacttc ttaaaagaga
    11281 tacctgaaaa aatattaaca aaatctatga gtttagagga tgtcaaaaaa cttataatga
    11341 ccgacgtttt accttaacat tattgtacgc actataaaca ataatgactt cttatcattg
    11401 gagtatattg cccatggata cagtgattga cctcttattt attatcgttc ctattatagc
    11461 cggatttctg gcatgcatta gtctttatcg ttattggaaa aaagatttcg aaagcgacga
    11521 ggacgatgat taaactcctc ggaatcaatg attccgagga gtttttctat attttcattt
    11581 aatcagcatt tctacgaatg cgattatagg ttttaaggat ttggctctta tgcttagtga
    11641 catagcgata atcttcttta agagtagcga tatcctttaa agctttcatg ccattacttg
    11701 tttgggcatc tttacgaata ggtcggttac tcgttgactg cccaaaggca ttttgaacat
    11761 ctaaagaaag cataaaatta ataaataact ttgcttcttt catagaagga gcattcttta
    11821 taattgcaac cgaagatggg acaaaaactg tcccttctgt cgggtataca atagaaacat
    11881 tggcaccact tttttgcaaa ttgacactag ggtcttcgta agtcaacccc acaatcattt
    11941 ttccttctgc aactgattga taaacttctg aagagctaga agatttgata gcattaatat
    12001 tatgttgtag ctttttaaca tagttccacg cttttggatt ggtgtaacca cccttggcca
    12061 agagtatatt agtgagttgt gagaaagcac tagaggaagt attcggatct gcaaaggcaa
    12121 ttttaccttt taaggaaggc tgtaataaat cttcataact cttgatggta agtcccttag
    12181 ctaattcgtt atttacaatc aagacactcc catttatagt ataaggtgtc gccgtatcac
    12241 tcggatggat atagtctgga ataacagtat gaacattctt tgatacgtaa gactcaaaca
    12301 atgccttatg actttcaaat tgcgtataat ttcctccaaa gaaaatatcc gccttcaact
    12361 gcttaccctc cttacttaat ctatctatta gttgccctgt cccaccttga ataagcttaa
    12421 cttttatacc gtatttttcc tcaaaagctg gaatcgttcc tgttaaaatg gcttgactat
    12481 ttggacttag aataactaat tcttttggtg gaagtagttt agaaggctgg ctaatactgt
    12541 atgtaaaaac acttataaag ataatgggaa caatcagtag tatataaata agccttttcg
    12601 actgtttttc tttcatcttt cacctaccta atccatctgt ttaaaaatat gttcttcctt
    12661 aaacgctttt actgtctgac ccaagtactt tttaaaaacc ctatcaaaat aacgataatc
    12721 ataaatacca acagcttctg caatttggta aaccattagg tctggttctc taaacatcaa
    12781 ctgaatggct tgattaatac gatattgatt tatataatca ctcaaagtta tatgaaggtg
    12841 ctttttagtg actgtgtaaa gataactttc actgtaacct aaatcgtgag ctaactggct
    12901 aatcacaatt ttcccatgaa aatggctatg aatccatgct agcatatctt taataacttc
    12961 aggaacttgg tcattagctt gtggtagagg taaatacaat tctgtatgct cttgtaggtg
    13021 cttctcctta tgttcctcaa ttctagccat aattgttttt aaacaatcaa tcaaagcttg
    13081 cttttctagt ggttttgtta aaaattcaag aacgccatag tgaatagctg attgcgcatt
    13141 tggaaaatca gcatagccag ataaaataat tttggcataa gattgtcctt tggttgcctc
    13201 aaacatatcg aatgctgtca taattggcat attaatatca cttaaaacaa tgtctggctg
    13261 ctcttcctga attaattgag ctccttcttt accatctttt gcttcaccaa ccactaaaat
    13321 attcaatgat tgataatcaa tagcatagcg caaccactta cgaataaggt gctcatcctc
    13381 aacgataagt aatctataca taactcactc ctttactata tagctaactc gaaaagcatc
    13441 atctgtcttc gtaaactcta acaatactgt tgaaaattga tattgtaatc gtctataaga
    13501 attaataagc ccatgatgcg agtgatttga tcttaacatt gtacgtatag ctttttgcct
    13561 agccaaacta atacccgatc cattatttga aacagtaaac caaattccat cactatcttg
    13621 ccaaatatct atgtttattg ctacatcatg acgttctttt aagccatatt taatagcatt
    13681 ctcaattagc ggtaacaaga aaagttttgg tacacgcatg tgttcaagtt caggagatac
    13741 tgaaatagtg taagttaatt cttcaaatct cacttgatta atcaaaaggt aggattcaat
    13801 gattgctaaa tcatccttaa gcacagctgc ctctgtgcta ccactaaggc tatagcgcaa
    13861 taattttgtt agttgtataa cgattctttc tgttaattgt gaatcatagt gactggttat
    13921 taaaattgtc tcaagggtat tataaaggaa gtgaggatta aactgagctt ctaacatcct
    13981 cttttcaaac agcaactttt gtgtttccaa atcgagagtt ttctcatgta aatcttttaa
    14041 ccgtgaaacc atctgattga tttgaacaga tagatattga aattcatctt gactatcaag
    14101 ctcaatacta gatttttctt ggcgggaaat ggcatccata tctagcacca tttgatttat
    14161 ggcgccagaa ttttttactg cgatgcgatc ggctagaact ctagattttc gtcgtaaaat
    14221 cacaaaaata ataactgatg atactagaga aaaaaggacg accagagtta ctggtatcag
    14281 gggacgatac atataaagta ggatattatc ttgcagagct actttcctaa caacaaaagc
    14341 tcgatgatcg tgaaaactaa aatatctagt taagaattga ctatctacct tatctaaact
    14401 agaggagata aaatcacgat tagtaaaagt aaagctatta tttagctgat cggcaataat
    14461 caagtctgag ttaatagcct tcgttggaag taaaaagtct ttcccattca tcaataaaaa
    14521 ggcataacct gaaacttttt tattaactat aattggctta atgagtgcaa ggtaatgccc
    14581 tccctcacta tctacaataa tttttagtag cctttgattt attttagcct tatctaaaac
    14641 ttcactaatg taaatagact tactaaaaaa attacctaaa tgtgaattac tagcaaacaa
    14701 aagttcttga ttaggtgaga atactaaaag ttctgtttga ctttgtaaat gattccttaa
    14761 atgataatat tgcttactca aatcagcact tcctaagtgt cctgagacaa atggtttcaa
    14821 actattttta cctaagtcat ctaataaggt atcatgtttt tgaatcaatt gcttataact
    14881 ataggaaact gaattaacat ctttataaag gcctatctgc tgaggcgcta aagaaaaaag
    14941 gacgaaaagt gcaatcaata acaaggataa aattaaagac tggcgggaaa aatgtttaga
    15001 gatatcatct tgtagtcgtt tcttaaagcg ttcttccatt ctatatcctc tcatttccat
    15061 ctccttttat tatagcaaat agctatccaa taaagatagt caactccttt catacaataa
    15121 ctttcaaaag aaaaaaagat aagctaaaca agtatcgtct tgttccactt acctttaatg
    15181 ctaggagatt attagcaaat acctaagaga ccaagtacaa tacctaacac cataatacca
    15241 aacatgatcc agttgatatt gactttttta cgtagaagcc agaaggctaa tatcgttacc
    15301 aacagtggca ctacgccaac aaataattgg tcaaggtaac tttgtatgga agctgcttcc
    15361 ttagctcctt tagctgctac cgttaagatt gtcttgaatt taacatttga agcagtcatt
    15421 gagcctacca tcattaaacc aagcatactt gaagtcttag taacaatttt aataccacct
    15481 gattcataca attttttgat aaaccctgca cctacagagt aacctccata gagactataa
    15541 taatgaatca gaaaagctgg aatgttatac aagagtagaa agacaacagc tcccatggct
    15601 gagcctgtac ttgctaagga gataccgata cctgccgcaa taacccttag aattccccaa
    15661 aagaatgaat caccaacacc agatataggt cccatcaaag aagccttaac tgcggtgata
    15721 gcactagcgt caaaatcagg atcttcactg tttttctttt ccatagatgc aacaagaccc
    15781 ataataaaat tattaatgtg catggtggca ttaaaccatg ttgtatgacg gaccagtgcc
    15841 tctgctttat ccttatcagt cttataaaaa cgattgatga ctggtagtaa agtataaata
    15901 actcctactg cgtggtattg tgttgcccca gtacgactgg cattcatcgt ccatgagcgc
    15961 cagaagactg agcgaagcat tttacggtct tctttagtca aattatcctg tgatttcatg
    16021 cgaagaagtc ctcctcttct gattctttag agtcaaaggt tgtctgttta ctgatcgcac
    16081 ctctagtaat tgcgtctaat tctatatcac gttgtgaact aatgacgcaa ataactgctc
    16141 ctagggcagc aactgcaacc gctggtaatt ttaaataagc tgttaaaaca aaaccaagca
    16201 agtaaaaaac agctaattta tttgtccata ataatttcat cagcatggca aaaccaacag
    16261 ctggaaggag gcctcccgcc gcactcaagc cattcatcag gttttgagga atatgatgga
    16321 caaaactatt aacaggccca ctacctacta aaataccaat gaaagaaata gaagcgataa
    16381 tgagatagta aataatccat gtaccatagt gaagcatcac aagtttacct tgatggtttg
    16441 cggctgcggc tttatccacc atcggtgcaa agatattcat gaagacgttt ttcaaaaaca
    16501 taacaacaaa tgctgccaac attccaatcg gaacagctag agtcattgct gctttttgat
    16561 caatgtttga aataatggta aaagttgttg ccatcgctgt tgctgttact ggctctgcgg
    16621 caataacacc cccaatatta acgtttccca aaaatagcgc ttctagggaa gcacccatta
    16681 agataccaac cttaatatca cctagtagca ttcctgttac aagaccaact acaagtggtc
    16741 tctccatcat actttgacct gttaagtaat ttcccccaaa acaaatcaac acagctaaag
    16801 ctgccatcgt cgctggcatt atcatatgtc atacctcact ttgtttgact agctgactca
    16861 agttaacctt agtatttgaa ggcaatactt ggttaaagac gtctaaatct ggtaagctgg
    16921 ctaattcttt tgcagcttcc aactcttctg gatttaataa gagacttgat gttagtttga
    16981 cttttgtagc tggatcagac ttatcaaagc gtcctacatt agcgacgttt actgcttcta
    17041 aatcggttat atttttagca attgtacagg catctttaac agaattgaca atcacaaaaa
    17101 tgcgcttgtc agttgcgcgt ggatctttaa atagagctat tgattcttca actgaacgga
    17161 ttaataattt catgccatta ggcacagcca ttttaagagt catctgtgtg atttcatttt
    17221 tggcagcctc atcattagcc accactaaaa gtggtgcatt taattcttta gtccatacaa
    17281 ctgccacttg cccgtgaatc aaacggtcgt caacacgaat ttgagtaatc atttttttcc
    17341 tcctaattat taatcgaaaa agtcatcttc tgaacacgct ttaaagctga gatctaccag
    17401 ctgcacttga gattcattga taacggttac aatttcttct ttagttaatt cctcttcagg
    17461 tttcaaaaat agtaatgaca acaaggtcgc taaattgctg ttcgttatta aaaaaacatt
    17521 atttttccca ctattcatta cagcagtcgc cattttctga ttaacgctgc cacctaacaa
    17581 atctgtaaaa ataagcccct gttcatctgg tgcaatctca tcaataaatt ctacaataga
    17641 cttggtatag tcactatcat caatgtaagc atctattgtc tgaatctctt gtcccttgcc
    17701 tgtcagaata tcaatagaac tctgcaaccc actcgcaagt tttccgtgac tgccgattag
    17761 gaatttccgc ttcatctttc tcctcctatt tattacatta ccctaaatta gttctgaaag
    17821 ttttatcaat actttgaata agataacctt tactatcatt ctaacaaatg aaaacggttt
    17881 aaaaatggac tattctactc tatgtgtgga ctattctata taattaatat ttaaaaacag
    17941 aacaataaaa aggtcatgta tcctaatagt ttacacaacc tttacatcac tataacttta
    18001 aactttctct tatatgaatc actatttcga aagagataat ggaataattt tttgataggg
    18061 tatcttatta acaaccttta ttatgagtag agcacaagca tgtgttaatg ccaaggtcac
    18121 cacaaaaatc acttttcctt gatggtgtgc aactaaggaa gttgatttta aacagtaaga
    18181 cactatgcca acaaaaagag ggtgaaggaa gaaataatag acactcaact gttttaaagg
    18241 acttaaacga tagcccttaa acagtgatgt acgtatactc cagttaaata agaatactgc
    18301 acataaagga gctaataaga agaaattttt atccaaccct tgattaaaat aaataagaac
    18361 attctctaaa gctagaagta aaaggaagag aatatactta agaccacgcg atttggtaaa
    18421 taggtcagta ttaaaataat catagagtaa atatcctgct agaagataaa ctggagtgta
    18481 aaataatccg tttcgtgttg tctgaaaaat tgacatataa gtggacatca ggggataaaa
    18541 ttttgttgac tgtacaaaga cagaataggt ctctactgct ccaatacaat agagtacaac
    18601 aactgtgata aatgtccccc tagttcctac atacttcaat aatccttgaa taatgaacca
    18661 acctagaata acagcaggga tgtaccaaag agtatgtgac atacctaaat acaaaaatgc
    18721 cacaataaaa cccggcagca aataaatctt cgctatattt agagattcaa aaaagaaata
    18781 accataaggc aaataaatga tagtccacat gctatacttt ttaatcagtt tacgaaagta
    18841 ggagtgatta cacaaacctt gctgtatacg acctctcaaa aagaaagcag tacaaatcaa
    18901 aaaataagga acagccatcc gacctaggaa acttttaaag gtaaaatgaa tgacatcctg
    18961 actaaataac cttccactat gaactaaaat gaccagtatg gaaaaaagat attgtagcaa
    19021 gctaatcatt gatgtattat gctgcttatt atccatgcac tgacttctct tcatccttaa
    19081 cattactttc tataagttat cattactatg ataccaatct cttcagaaga atatctcaat
    19141 tatccccaaa agtcattgaa acgccttaaa tggtatctga taattattgt taatacctta
    19201 tcaaccatat taggaaataa aaaagctgat ttcgcaaaag aaaatcagct tagaataatt
    19261 tcttgttcag ataaccagtg ggaactataa acttcttcgt cgcattaaac gataggtata
    19321 agctatcaat aacactgcaa ttaacatacg tagactaatc agacagattt cattagcaga
    19381 aaaactatca aagaaaaagc catgactacc attacgtgat aattttaaaa gtaaagctga
    19441 agtgtctgtc gttgatactg agaaaatagt taataacttg tttatcacac ctttattaaa
    19501 catagccatt aaaaagctag gagcaaaaaa ggcaagcgtt gaaacagcaa aagatgttaa
    19561 tggcgaattt gtgatactta atataaagaa attaagactg gctataaaaa ggagtccaac
    19621 aaattgtaag acaataatat gaacaaaagc agttgatatt gtcataggta caggaaatcc
    19681 cataatatta gccaaaggtg atgtccagta taaattcagt tgaacacttg tctcccaacc
    19741 actaaaacca aaataatctg caaataccgt cagaatgagg gcatatgcta ggataaatgt
    19801 ggaaataccg atggtcattg tactagctaa cttagcccag gtcaactgac cacgtccata
    19861 ccgagtcgtc agtagtatag gattaatctt cttagcccta tctcctgaaa aaagaggagc
    19921 tgataagaat agagtaaata agacaattaa aatataagta ctattactta cttcaaaaag
    19981 ttgattccaa ggagcaaagt tacctagctt tagttgtttt agtggtagcg cagaacccaa
    20041 ctgtttttga tcttt
//
LOCUS       DATROD010000023        19448 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.023, whole genome shotgun sequence.
ACCESSION   DATROD010000023 DATROD010000000
VERSION     DATROD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19448
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.023"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..324
                     /locus_tag="VBC74_000843"
     CDS             <1..324
                     /locus_tag="VBC74_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6941808.1"
                     /translation="QQNLTIEFLADYMGYSKTHFMTVFKQHTGTSCTEFIIQVRLNKA
                     SEHLINSTTAIIDIANSVGFNNLSNFNRQFKRYYHTTPRQYRKQFTNKKDGSTLVYRN
                     INTKK"
     gene            complement(381..1913)
                     /locus_tag="VBC74_000844"
     CDS             complement(381..1913)
                     /locus_tag="VBC74_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEO6941809.1"
                     /translation="MENHKNEFQFSLESILGFVWRGIVVGLIAGFVVSIFRLAIEKIF
                     LVVMELYKSAHYQPIILLSITVTSIIAAVIIGFFIKSDPDIKGSGIPHVEGELKGMLS
                     PDWFSIVWKKFIAGILAISSGLMLGREGPSIQLGAMTGKGIAQYLNASRMEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLVWITALVASLVANFVSLNIFGLTPVLA
                     LPSELPSLNLNFYWIFLLMGLFLGILGFIYEWVILRFHVIYDYLGKLFHLPSHLYGIL
                     AVIFILPIGYYFPQLLGGGNGLIVSLPRSNLSLMMLGLFFLIRFLWSMLSYSSGLPGG
                     IFLPILALGSLAGAFFAVGMQYFGIISHQQISLFVVLGMAGYFGAISKAPLTAMILVT
                     EMVGDLKQLMAIGIVTMVSYIVMDLLKGEPIYEAMLAKMTFNPKDKVMTPTLIELTVS
                     DKISGKYVRDLELPENVLITTQIHHKTSAVVSGNTILNAGDTIFLVVNESEIKEVREQ
                     LM"
     gene            complement(2022..3095)
                     /locus_tag="VBC74_000845"
     CDS             complement(2022..3095)
                     /locus_tag="VBC74_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6941810.1"
                     /translation="MRRVYSFLGGIILVILILFGLTTYLEKKSSSTPNSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMHTKIKQGGTTYDIAVPSDYMIDKMIKEN
                     LLVKLDHSKIANWDAIGARFKNLSFDPKNKYSIPYFLGTVGIVYNDQLVKTPPKHWDD
                     LWRPEFRNKIMLVDSAREVIGVGLNSLGYGLNTKNISELKAASKKLDALTPNVKAIVA
                     DEMKGYMIQGDAAIGVTFSGEAREMLDGNKHLHYVVPSEGSNLWFDNIVIPKTVKHRK
                     EAYAFINFMMEPKNAAQNAEYIGYATPNLKAKALLPADIKNDKAFYPPDKTIDHLEVY
                     NNLGQKWLGIYNDLYLQFKMYRK"
     gene            complement(3088..3864)
                     /locus_tag="VBC74_000846"
     CDS             complement(3088..3864)
                     /locus_tag="VBC74_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6941811.1"
                     /translation="MKKFANIYLAFVFIILYIPIIYLIFYSFNKGGDMNSFTGFTFSH
                     YGELFQDSRLMLILVQTFFLAFLSALLATIIGTFGAIWIYQVRRRHQTSILSLNNILL
                     VAPDVMIGASFLLVFTVIGLQLGFTSVLLSHVAFSIPIVVLMVLPRLKEMNDDMINAS
                     YDLGASTWQMLKEVMLPYLSSGIISGFFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARRGISLEINALSTIVFLFSILLVIGYYYISKEKGEKNA"
     gene            complement(3861..4655)
                     /locus_tag="VBC74_000847"
     CDS             complement(3861..4655)
                     /locus_tag="VBC74_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6941812.1"
                     /translation="MKKTSSLFSIPYMAWLFLFVLAPVALIAWNSFFDINGHFTLANY
                     QTFFSSGTYLKMSFNSVLYAGIVSFITLLISYPAAYLLTKLKHKQLWLMLIILPTWIN
                     LLLKAYAFMGIFGQQGGINAFLTFIGIGPKQILFTDFSFLFVAAYIELPFMLLPIFNA
                     LDDIDQNLIYASDDLGANAWQTFQKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLITQNKGMGSTIGVILILVMVAIMWLTKERKA"
     gene            complement(4639..5793)
                     /locus_tag="VBC74_000848"
     CDS             complement(4639..5793)
                     /locus_tag="VBC74_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6941813.1"
                     /translation="MTNPIIAFKNVSKVFEDSNTVVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLEASTGDIYLDGKRINDVPTNKRDVHTVFQNYALFPHMTVFENVAF
                     PLKLKKMDKKEIQKRVQETLKMVRLEGFEKRAIQKLSGGQRQRVAIARAIINQPKVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNEGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILSGKMIEDYLVEFNGKRFEAVDGGMRPNESVQV
                     VIRPEDLQITLPDEGKLQVKVDTQLFRGVHYEIIAYDDLGNEWMIHSTRKAIEGEVIG
                     LDFTPEDIHIMRLNETEEEFDARIEEYVDTDDHEDGLINAIEEERNEENL"
     gene            complement(5842..6744)
                     /gene="murB"
                     /locus_tag="VBC74_000849"
     CDS             complement(5842..6744)
                     /gene="murB"
                     /locus_tag="VBC74_000849"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEO6941814.1"
                     /translation="MIKTIQKELEGLDIRFDEPLKKYTYTKVGGPADYLAFPRNRLEL
                     SRIVKFANSQNIPWMVLGNASNIIVRDGGIRGFVIMFDKLSTVTVNGYVIEAEAGANL
                     IETTRIARYHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILLSAQVLTPQGELKT
                     IEARNMQFGYRHSVIQESGDIVISAKFALKPGDHLMITQEMDRLTYLRELKQPLEYPS
                     CGSVFKRPPGHFAGQLISEAHLKGQRIGGVEVSQKHAGFMVNIAEGSAQDYENLIEHV
                     INTVESTSGVHLEPEVRIIGESLL"
     gene            complement(6888..7376)
                     /gene="folK"
                     /locus_tag="VBC74_000850"
     CDS             complement(6888..7376)
                     /gene="folK"
                     /locus_tag="VBC74_000850"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEO6941815.1"
                     /translation="MTTVYLSLGSNIGDRETFLKQALFSIDHLQKTKVAQISAIYETA
                     AWGNTNQEDFFNICCQVETDLAPFELLDYCQEIEKRLKRVRHEHWGPRTIDIDILLFG
                     NQVINQEDLVVPHPYMTKRAFVLVPLLEIAPQLSLPNGSKLEDYLEKLNLGEVHYLKP
                     VE"
     gene            complement(7373..7735)
                     /gene="folB"
                     /locus_tag="VBC74_000851"
     CDS             complement(7373..7735)
                     /gene="folB"
                     /locus_tag="VBC74_000851"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEO6941816.1"
                     /translation="MDKIYLNKCRFYGYHGAFSEEQTLGQVFQVDAVLSLDLAKASQT
                     DDLIDTVHYGEVFDCIKNHVENEQYQLIEKLAGVIVEDIFLQFHPVQAITLKITKDNP
                     PINGHYESVGIELERKRV"
     gene            complement(7737..8540)
                     /gene="folP"
                     /locus_tag="VBC74_000852"
     CDS             complement(7737..8540)
                     /gene="folP"
                     /locus_tag="VBC74_000852"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEO6941817.1"
                     /translation="MKIGQYDITGKACIMGILNVTPDSFSDGGSYTTIDSALNQVGEM
                     LEQGVAIVDIGGESTRPGAVFVTAEEEIKRVVPMIKAIREVYPDLLLSIDTYKTEVAQ
                     AALDAGVHILNDVWSGLYDGKMLSLAAERNVPIILMHNQEEAVYQDIKKEVCEFLLER
                     AERALEAGVSKDNIWIDPGFGFAKTEEQNLELLKGLEQVCDLGYPVLFGISRKRTVNY
                     LLGGNREVTERDMGTAALSAWAIAKGCQIVRVHNVEVNKDIVTVISQLV"
     gene            complement(8544..9107)
                     /gene="folE"
                     /locus_tag="VBC74_000853"
     CDS             complement(8544..9107)
                     /gene="folE"
                     /locus_tag="VBC74_000853"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEO6941818.1"
                     /translation="MPNQEKMEKAIYQFLEALGENPDREGLKDTPKRVAKMYIEMFSG
                     LNQDPKEQFTAVFSENHEEVVIVKDIPFYSMCEHHLVPFYGKAHIAYLPNDGRVTGLS
                     KLARAVEVASKRPQLQERLTAQVAQALEDALAPKGIFVMIEAEHMCMTMRGIKKPGSK
                     TITTVARGLYKDDRYERQEILSLIQKG"
     gene            complement(9126..10388)
                     /locus_tag="VBC74_000854"
     CDS             complement(9126..10388)
                     /locus_tag="VBC74_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEO6941819.1"
                     /translation="MTYQEALEWIHSKLAFGIKPGLERMRWMLEQLGNPQNNLSAIHV
                     VGTNGKGSTTSYLQHIFTNSGYQVGTFTSPYIVDFRERISIDGQMIPESDFIKLVETV
                     RPVVERLHLETNLEPATEFEVITVLMFYYFGNSCPVDIVIIEAGMGGYYDSTNMFKAL
                     AVTCPSIGLDHQEVLGRTYVDIAEQKVGVLKKGVPFVYANDRQDVEEVFQIKAKETHS
                     QTYRLHNDFYIKEEENYFNYIGPQANIDHIQLQMPGHHQVSNASIAITTSLLLRDKYP
                     KLTLQTIKDGLEMTKWVGRTELIFPNVMIDGAHNNESVDALVQVIKKYQQKNVHILFA
                     AINTKPIESMLESLSSIAPVSVTSFDYPKSINLDKYPKAYTRVSDWKKWLHDINLTSD
                     KDFYVITGSLYFISQVRQELLLIKTTTT"
     gene            complement(10390..11277)
                     /gene="rarD"
                     /locus_tag="VBC74_000855"
     CDS             complement(10390..11277)
                     /gene="rarD"
                     /locus_tag="VBC74_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEO6941820.1"
                     /translation="MRKDNLGIILGLSAYVLWGLLSLYWKLLSGIEAYSTFAYRIIFT
                     VLTMLIYMLVSGRKTVYLKDLKGLVNNKKSFWTMFVASILISINWLVYIFAVTHGHAT
                     EASLGYYMMPIISILLSVLVLREHLARVVSLAILIAIMGVGILVYQTGHFPLISLTLA
                     LSFGFYGLLKKSISLSSDFSMLVESSFIAPFALIYIVFFAKDFLTDYNILQLVLLSLS
                     GIITAVPLLLFAEAIKRAPLNIIGFIQYINPTIQLLLALFIFKETIVSGEVIGFIFIW
                     LAILVFSIGQVHTMLKKGK"
     gene            complement(11264..12130)
                     /locus_tag="VBC74_000856"
     CDS             complement(11264..12130)
                     /locus_tag="VBC74_000856"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEO6941821.1"
                     /translation="MRIIVPATSANIGPGFDSIGVALSKYLSIEVLEESTEWLVEHNL
                     VNIPKDHTNLLIQTALHVKSDLAPHRLKMFSDIPLARGLGSSSSVIVAGIELANQLGN
                     LALSQKEKLEIATRLEGHPDNVAPAIFGDLVISSIVKNDIKSLEVMFPDSSFIAFIPN
                     YELKTSDSRNVLPQKLSYEDAVASSSVANVMVASLLKGDLVTAGWAIERDLFHERYRQ
                     PLVKEFGVIKQISTQNGAYATYLSGAGPTVMVLCSKEKEQPIVTELSKLCLDGQIQVL
                     NIERKGVRVEKR"
     gene            complement(12132..13415)
                     /locus_tag="VBC74_000857"
     CDS             complement(12132..13415)
                     /locus_tag="VBC74_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEO6941822.1"
                     /translation="MTIKIALLGFGTVAKGIPYLLKENQHKLLSLEGEDIVIDKVLVR
                     DNESRQRFINQGFTYNFVTEINTILQDPQIDIVVELMGGIEPAKTYLSQALGFGKHIV
                     TANKDLIALHGKELMDLADARGLALFYEGAVAGGIPILRTLSHSFASDKMTRLLGILN
                     GTSNFMLTKMFEEGWSYEQALKKAQELGYAESDPTNDVEGIDTAYKATILSQFGFGMP
                     IDFDDVNYKGISSIRSEDVEVAQEMGFAIKLVTDLRETPTGISVDVSPTLISQEHPLA
                     AVNHVMNAVFIESIGIGQSLFYGPGAGQNPTATSVLADIIDISRSIRSQIKIKPMNTY
                     HCPCRLSMQSDIFNEYYLAISLRNAEDSDTLGRYFEQENIGLKNVIEKALDDKQQEIY
                     VLTDEVSQEKITQFIEEFPESGVIQLINVFKVIGG"
     gene            13592..14479
                     /locus_tag="VBC74_000858"
     CDS             13592..14479
                     /locus_tag="VBC74_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO6941823.1"
                     /translation="MAHTPTSHRKPRKRSPWLAIASVFFLLIALIGIFLFFNNRSKQE
                     IKTKTNASSHRKIVTSIKKKKWVKQKTPVKIPILMYHAVHVMDPSEAASANLIVAPDI
                     FESHIKRLKKEGYYFLAPNEAYRALNENALPEKKVIWITFDDGNADFYTKAYPILKKY
                     KVKATNNIITGFVQEGRESNLNVQQMLEMKQNGMSFQGHTVTHPNLSLLTPELQTQEM
                     TLSKLFLDQKLSQDTLAIAYPSGRYNPTTLDIASQYYKLGLTTNEGVATKDNGLLSLN
                     RVRILPTTSDDDLIKTINQ"
     gene            complement(14502..16049)
                     /locus_tag="VBC74_000859"
                     /pseudo
     CDS             complement(14502..16049)
                     /locus_tag="VBC74_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
     gene            complement(16192..17568)
                     /locus_tag="VBC74_000860"
     CDS             complement(16192..17568)
                     /locus_tag="VBC74_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEO6941824.1"
                     /translation="MAYKTIYPYTNEVLHEFDNISDSDLEQSLDIAHALYKTWRKEDN
                     VEERQNQLHKVADLLRKDRDKYAEVMTKDMGKLFTEAQGEVDLCADIADYYADNGQKF
                     LKPVPLESPNGEAYYLKQAVGVLLAVEPWNFPFYQIMRVFAPNFIVGNTMLLKHASIC
                     PASAQAFEDLVREAGAPEGAFKNIFASYDQVSNLISDPRVAGVCLTGSERGGASIAAE
                     AGKNLKKSSMELGGNDAFLILDDADFDLLSKTIFFARLYNAGQVCTSSKRFIVMADKY
                     DEFVNMVVETFKSAKWGDPMDSETTLAPLSSAGAKDDVLKQIKLAVDHGAEVVFGNDT
                     IDHPGNFVMPTVLTNITKANPIYNQEIFGPVASIYKVDTEEEAIALANDSSYGLGSTV
                     FSSDPEHAKKVAAQIETGMTFINSGWTSLPELPFGGIKNSGYGRELSQLGFDTFVNEH
                     LVFTPNSD"
     gene            complement(17642..18649)
                     /locus_tag="VBC74_000861"
     CDS             complement(17642..18649)
                     /locus_tag="VBC74_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEO6941825.1"
                     /translation="MLFKEKIPGLILCFIIAIPSWLLGLYLPLIGAPVFAILIGIIVG
                     SFYQNRQLFNKGIAFTSKYILQTAVVLLGFGLNLMQVMKVGISSLPIIIMTISISLII
                     AYVLQKLFKLDKTIATLIGVGSSICGGSAIAATAPVINAKDDEVAQAISVIFLFNILA
                     ALIFPTLGNFIGLSDHGFALFAGTAVNDTSSVTATATAWDAINHSNTLGGATIVKLTR
                     TLAIIPITIVLSIYHMKQTQKEQSVSVTKIFPKFVLYFILASLLTTIVASLGFSLRIF
                     EPLKVLSKFFIVMAMGAIGINTNVSKLIKTGGKSILLGAACWLGIIIVSLTMQAILGT
                     W"
     gene            complement(18757..19443)
                     /locus_tag="VBC74_000862"
     CDS             complement(18757..19443)
                     /locus_tag="VBC74_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941826.1"
                     /translation="MKKFLKVFLTVLVLILIVGAGGLYFWNNRQSLEGKWRTVSLEKQ
                     VEKEIEQQLGSQAADMGISAADLVKGANMHMNVKNDEAKITVTAQIDEVKFHQAIKTF
                     IDKALEKQLKDQGLTYNDLSEAGKKIFDETKITDKQIDQQIDRSFQSAAQAVGGKYNT
                     NTGEMTLPVMDGKVHRLTSVIKVSHINKKANAFYGNIVKNGEKTAYKKEGSKLILGNE
                     KSYPFMKVTK"
BASE COUNT         6585 a         3928 c         2920 g         6015 t
ORIGIN      
        1 cagcaaaatc ttaccattga atttttagct gattatatgg ggtatagtaa gacacatttt
       61 atgaccgtat ttaaacagca tactggaaca tcctgtacag aatttatcat acaagttcgt
      121 cttaataaag ctagtgagca tcttattaac tccactacgg ccattattga tattgccaac
      181 agcgttggtt ttaataatct atcaaatttt aatcgccaat ttaaacgcta ttatcataca
      241 actcctcgcc agtaccgcaa acaatttacc aacaaaaaag acggtagtac gcttgtctat
      301 agaaatataa atacaaaaaa atagaaacta gttaaatcca tgactagttt ctatttttta
      361 ttatttaagc gttaattagt ttacatcagc tgctcacgta cttctttaat ttccgattca
      421 ttcacaacta aaaaaatggt gtcgccagca ttcaagatag tatttcctga tactacagcc
      481 gatgtcttat gatgaatttg agtcgttatt agcacatttt ctggtaattc taaatctcgg
      541 acatactttc ctgatatttt atctgaaaca gttaattcaa tcaatgtagg tgtcattact
      601 ttatcttttg gattgaaggt cattttagcc aacatcgctt cataaatagg ttctcccttt
      661 aataaatcca tgacaatata agataccatt gttacaattc caatagccat taattgtttt
      721 aaatcgccaa ccatttctgt aaccaaaatc atagctgtta gtggtgcttt tgagattgca
      781 ccaaaatagc ccgccattcc tagaacaaca aataaagata tctgctgatg agatattata
      841 ccaaagtact gcatacctac tgcaaaaaaa gcacctgcta aagatcctaa agctagaatt
      901 ggtaaaaaaa ttcctcctgg caagccacta ctgtaactca acatactcca taaaaaacga
      961 atgaggaaaa ataatcctaa catcatcaga ctaagattac tcctaggcag tgatacaatt
     1021 aagccatttc caccccccaa taattgtggg aaatagtaac caataggaag aataaaaatt
     1081 accgctaata taccatatag gtgagatggt agatgaaata gtttacctaa ataatcatag
     1141 atcacatgaa atctcaaaat tacccactca tagataaagc ctaagatacc caaaaagaga
     1201 cccatgagta aaaaaatcca ataaaagttg agatttaaag atggtaattc tgatggtaaa
     1261 gctaaaactg gggttaaacc aaagatatta agtgagacaa agtttgccac taaacttgct
     1321 accaaagctg ttatccaaac taagcgagaa aaatgatggt aaatttcttc aactacaaat
     1381 aaaaggcctg caataggagc attgaaggct gctgaaagac ctgctgctgc accactagca
     1441 attaaaacac gtttttccat tcgactagca ttgagatact gtgcaattcc cttacctgtc
     1501 atagctccta actgaatact gggaccttcg cgaccaagca taagtcccga agaaatagct
     1561 aaaatacccg caatgaattt cttccataca atactaaacc agtctggcga tagcattcct
     1621 ttaagttcac cttcaacatg cgggatacct gaacctttaa tatcaggatc agatttgata
     1681 aaaaaaccta ttatcactgc agcgataata ctagtaaccg taatgctcaa taaaataatt
     1741 ggttgataat gggcactttt ataaagttcc ataacaacta aaaaaatttt ttcaatcgct
     1801 aatcgaaata tgctgacaac aaatcctgct attaagccta ctactattcc tctccaaaca
     1861 aatcccagaa ttgattcaag tgaaaactga aattcatttt tatgattttc catattttcc
     1921 tccttttctt acaattattt taacaaaaat atcacaaact ttctaggaac tcttggtaac
     1981 taaaaatacc tctcatcaga gaagtacttt tagttaatag attacttacg atacatttta
     2041 aattgtaagt acaaatcatt ataaatacca agccattttt gacccaagtt attataaact
     2101 tctaaatgat caattgtttt atcaggagga taaaatgctt tatcattttt gatatctgct
     2161 ggtaacagcg cttttgcttt gagattaggg gtcgcataac caatatattc tgcattttgt
     2221 gccgcatttt taggttccat catgaaatta ataaaggcat aagcttcttt gcgatgcttt
     2281 actgttttgg ggattacaat attatcaaac caaaggttcg atccttcact aggaacaacg
     2341 taatgcaaat gcttgttccc atcaagcatt tctcgtgctt ctccagagaa tgtaacacca
     2401 atcgctgcat ctccctgaat catatagcct ttcatctcat cagcaacaat agccttaacg
     2461 tttggtgtta gagcatctaa ctttttagaa gcggctttta attctgatat gttctttgta
     2521 ttcaatccat atcccaagga atttaaacca actccaataa cctcacgtgc actatcaact
     2581 agcatgattt tatttcgaaa ctctggacgc cataaatcat cccaatgttt aggaggagtt
     2641 ttaactaatt gatcattata aacaatccca acagttccca aaaaataagg aatcgaatat
     2701 ttatttttag gatcgaaact caaattttta aacctagctc caatagcatc ccaattagct
     2761 atctttgaat gatcaagttt aaccagaagg ttttctttta tcattttgtc aatcatataa
     2821 tcactaggta cagcaatatc atatgtcgtc ccaccctgct taattttggt gtgcatagcc
     2881 tcattagagt caaacgtttc gtattgtact tcaattccag tttcttttgt aaattttttt
     2941 aataaggctg gatcaatata gtctccccag ttataaataa ctaacttatc tgagttaggt
     3001 gttgaactac tcttcttttc taagtaagtg gttaaaccaa agaggatcag gataacaaga
     3061 ataataccgc ctaaaaagga ataaactcta cgcattcttt tctcccttct ctttcgaaat
     3121 atagtaataa ccaattacca ataaaatact aaataaaaag acaatagttg acaaggcatt
     3181 aatttccaat gatataccgc gacgtgcgcg tgaatagatt tctactgaaa gcgttgaaaa
     3241 gccattacct gtaacaaaga aagtaaccgc aaaatcatct agcgagtaag tgaaagccat
     3301 aaagaaacct gaaataatac ccgacgacaa atagggtaac ataacttctt taagcatttg
     3361 ccaagttgaa gctcctaaat cataggaagc attaatcata tcatcattca tttcttttaa
     3421 acgaggtaag accattaaaa caacaatagg aatagagaat gcaacatggc tcaataatac
     3481 agaagtgaaa cctaactgta aaccaataac agtaaatact aagagaaaac ttgcaccaat
     3541 cataacatct ggtgctacta aaaggatatt attaagcgat aaaatagagg tttgatgtct
     3601 acgtcttacc tgataaatcc aaatagctcc aaaggtacca ataattgtag ctaataaggc
     3661 gctaagaaaa gccaaaaaga atgtttgcac taaaattagc attaagcggc tatcctgaaa
     3721 cagttctcca tagtgactaa aggtgaatcc agtaaaagaa ttcatatccc cacctttatt
     3781 gaaagaataa aaaatcaaat aaataatagg aatataaagg ataataaaaa caaatgctaa
     3841 atagatattt gcgaattttt tcatgctttc ctctcctttg ttaaccacat aatagcaacc
     3901 atcactaaaa ttaaaataac ccctatggtt gagcccatac ctttattttg tgtgattaaa
     3961 aaatgttgtt ctatggctgt ccctaaagta ataactcgat taccaccaat aagacgcgta
     4021 agcataaaga gacttaaact aggaataaag acagattgta cacctgctct tacaccattc
     4081 agagatagag gaaatataac cttctgaaaa gtttgccatg catttgcacc caagtcatca
     4141 cttgcataaa tgagattttg atcaatatca tccaaggcat taaaaatggg taataacata
     4201 aacggtaatt caatgtaagc tgctacaaaa agaaatgaaa aatctgtaaa tagaatctgt
     4261 tttgggccga tacctataaa ggttaagaag gcgttaattc ccccttgttg accaaatatc
     4321 cccataaaag cataggcttt taacaaaaga ttaatccatg ttggcaaaat aattagcatt
     4381 agccataatt gcttgtgttt taattttgta agcaaataag cagctggata gctaatcaaa
     4441 agcgttatga aactaacgat gcctgcatat aggaccgagt taaagctcat tttaagatag
     4501 gttcctgagc taaaaaacgt ctggtaatta gctaaagtaa agtgcccatt aatatcaaag
     4561 aacgagttcc aagcaatcaa agcaacaggt gctaatacaa ataagaataa ccaagccata
     4621 taaggaattg aaaatagact agaggttttc ttcatttctc tcctcctcga tagcattgat
     4681 taaaccatct tcatgatcgt cagtgtctac atattcttca atgcgcgcat caaattcttc
     4741 ttctgtttca ttaagacgca taatatgtat atcttctgga gtgaaatcta acccaataac
     4801 ttcaccctca atagctttac gagttgaatg aatcatccat tcattaccta aatcatcata
     4861 agcaataatt tcatagtgga cacctcgaaa aagttgtgtg tccactttaa cttgaagttt
     4921 tccttcatcg ggtagggtaa tttgaagatc ttctggacga ataactactt ggactgattc
     4981 attagggcgc ataccgccat caacagcctc aaagcgctta ccgttaaatt caaccaaata
     5041 atcctcaatc atcttgccag ataagatatt tgattcacct ataaaagttg ctacaaaatg
     5101 attgataggt tcatcgtaga tatctactgg tgttcctgat tgaacaattt caccttcatt
     5161 catgacgaaa atccaatcac tcattgccaa cgcttcttct tgatcatgtg tgacaaaaac
     5221 aaaggtaatt cctaaacgtt gttgcagttc gcgcaattca tattgcatct ctgtacgcaa
     5281 tttcaaatcc aatgctgata agggttcatc tagtaagact actttaggtt gattgatgat
     5341 agcacgcgct atcgcaacac gctggcgttg tcctcctgat aatttttgaa tagctcgctt
     5401 ttcaaaccct tctaaacgaa ccatttttag agtttcttga acacgttttt gaatttcttt
     5461 cttatccatt tttttcaact ttaatggaaa agcaacgttt tcaaaaaccg tcatatgtgg
     5521 aaacaaagca tagttttgga aaacggtatg aacatcccgt ttattagtgg gaacatcatt
     5581 gatgcgtttc ccatctaagt aaatatcgcc tgtactcgcc tctagcaaac ctgcaataat
     5641 atttaagata gttgatttac cacttcctga ggcccctaat aaggtgtaaa atttaccttc
     5701 ttcaagttca aaattaatat cttttaagac aaccgtgttg ctgtcttcga atacctttga
     5761 tacattttta aatgcaatga ttgggttagt caattctcat aaatcctcca tttttctgac
     5821 taaagtaagt actaaactac tttataatag cgactcaccg ataatacgaa cttctggttc
     5881 caaatggaca cctgaagttg attcaacggt gttaataaca tgttcaataa gattttcata
     5941 atcttgagct gaaccttctg caatgttaac cataaaacca gcatgttttt gactaacctc
     6001 tactccacca atacgttgac ctttaagatg tgcttcgcta atgagttgcc ctgcaaaatg
     6061 tcctggagga cgtttgaaca cagaaccaca agaagggtat tctaaaggtt gtttcagctc
     6121 acggagatag gttaaacgat ccatttcctg agtaatcatg agatgatctc ctggttttaa
     6181 ggcaaactta gctgatataa caatatctcc actctcttga atgacagaat gacgataacc
     6241 aaattgcata ttcctagctt caatcgtttt gagttcaccc tgtggtgtca aaacttgtgc
     6301 agacaataaa atatgagcaa tttcaccacc gtaagcgcca gcattcataa agactgctcc
     6361 acctacactt ccaggaattc cgcaagcaaa ctcaaagcca gttaaactat gataacgtgc
     6421 aatacgagtt gtttcaatga gattagctcc tgcttctgct tcaataacat aaccgttgac
     6481 tgtaacagtt gacagtttat caaacatgat aacaaaacca cggataccac catctctaac
     6541 gataatatta ctcgcatttc ctaaaaccat ccaaggaata ttttgactgt tagcaaactt
     6601 aacaatacgc gatagttcaa ggcgattcct tgggaatgca agataatcag caggtcctcc
     6661 tactttagta taagtatatt tctttaaagg ctcatcaaag cgaatatcga gaccttctaa
     6721 ttctttttgt attgttttaa tcataacctt tatctcaatt ctgttttatt gtgttttata
     6781 gacacaaaaa atcggcataa aaccgatttc tattatacca aaaatatagg ttgttgacga
     6841 tacattatca caatttctgt gtattttaat tttaaaatcc taactgacta ttcgactggt
     6901 tttaaataat gaacttctcc taaattcaat ttttcgagat agtcttctag cttagaacca
     6961 ttaggtaaac ttagttgagg tgctatttcg agtaacggaa ctagaacaaa tgctcgcttc
     7021 gtcatatacg gatggggtac tactaaatct tcttggttaa tcacttgatt accaaaaagt
     7081 aagatatcaa tatctattgt ccttggaccc cagtgctcat gtctcacgcg ctttaagcgt
     7141 ttttcaattt cttgacaata atccaataat tcaaaaggtg ctaagtctgt ctctacctga
     7201 caacatatat taaaaaaatc ttcttggtta gtattccccc aagcagctgt ttcataaata
     7261 gcggaaattt gcgcgacttt tgtcttttgc aaatgatcaa ttgaaaagag agcctgcttc
     7321 agaaaagttt ctctatcacc aatattacta cctaaactca agtaaacggt tgtcatactc
     7381 gcttgcgctc caattcaatt ccaactgatt catagtgccc attaattggt ggattgtctt
     7441 ttgtaatctt taatgtaata gcttgtactg gatgaaactg taagaaaata tcttcaacaa
     7501 taacacctgc taatttctct attaattgat actgttcgtt ttcaacatga tttttaatac
     7561 agtcaaaaac ttcaccataa tgaaccgtat caattaaatc atcagtttgt gatgcttttg
     7621 ctaaatcaag tgatagaaca gcatcaactt gaaaaacttg ccccaatgtt tgttcttccg
     7681 aaaaagcacc atgataacca taaaaccgac atttatttaa ataaatctta tccatattac
     7741 accaactgac taattactgt cacaatgtcc ttatttactt caacattgtg aacacgtaca
     7801 atctgacacc cctttgctat tgcccatgcc gataaagcag cagtccccat atcacgttca
     7861 gttacttccc tattaccacc tagaagataa ttaacagtac gttttcgaga gatcccaaat
     7921 aacacaggat aacctaaatc acacacttgt tccaaacctt ttaatagctc taaattttgc
     7981 tcctctgttt tagcaaaacc aaagccagga tctatccaaa tattatcctt tgaaacacct
     8041 gcttctaaag cacgctctgc tctttcaagt aagaattcac aaacttcttt tttgatatct
     8101 tgataaacag cttcttcctg gttatgcatc aaaataattg gaacatttcg ttctgctgct
     8161 aaagatagca ttttaccatc atataaacct gaccaaacgt catttaaaat atgtacacct
     8221 gcatcaaggg ctgcttgagc tacctctgtt ttataagtat ctattgataa caaaagatca
     8281 gggtagacct ctctaatagc tttaatcatt ggaactacac gtttaatttc ttcttcagca
     8341 gtcacaaata ctgcacctgg ccgcgttgat tctcccccaa tatctacaat tgccacccct
     8401 tgttcaagca tctcaccaac ttgatttaaa gctgaatcaa ttgtggtata agaaccacca
     8461 tctgagaagg aatcaggcgt tacatttaaa atccccatga tacaagcttt acctgtaata
     8521 tcatattgtc ctattttcat cttttatcct ttttgtatta gcgatagaat ttcttgtctt
     8581 tcatatcgat cgtccttgta taaaccacga gctaccgttg taatcgtctt acttcctggt
     8641 tttttaatgc cacgcattgt catgcacata tgctctgctt ctatcataac aaaaatacct
     8701 ttaggcgcta aagcatcctc taaagcttgt gcaacttgtg ctgttaaacg ttcctgaagt
     8761 tgaggacgct tactcgccac ttcaactgca cgtgcaagct tactaagtcc agtaacacgt
     8821 ccatcgtttg gtaaataagc aatatgagct ttaccataaa aaggaaccaa atgatgttca
     8881 cacatggaat agaatggaat atccttgaca atcacaacct cttcatgatt ttcagaaaag
     8941 actgcagtga attgctcctt tggatcttga ttaagtcctg agaacatttc tatatacatt
     9001 tttgccacac gcttcggtgt atctttcaac ccttctcgat caggattttc tcctaaagcc
     9061 tctaaaaatt gataaatagc tttttccatt ttttcttgat tgggcattaa tttatcctat
     9121 cctttttaag ttgttgtagt tttgattaat agtagttcct gacgaacttg agaaataaaa
     9181 taaagggagc cagtaatcac atagaaatct ttatctgatg tcagatttat atcatgtagc
     9241 catttcttcc aatcactaac tctagtgtac gctttaggat atttgtctaa gtttattgat
     9301 ttagggtaat caaagctagt aaccgaaacc ggagcgatac tagacaatga ttctaacata
     9361 ctctctatcg gtttagtatt aattgcagca aatagaatat gcacgttttt ctgctgatat
     9421 ttttttataa cttgaacgag agcatcgaca ctttcattat tatgagcgcc atcaatcatt
     9481 acatttggaa agattaattc tgtacggcct acccattttg tcatttcaag cccatcttta
     9541 atggtctgta gggttaactt tggatatttg tctcttaata ataaactggt agttatagct
     9601 atgctagcat tggaaacttg gtgatgtccc ggcatttgca actgaatatg atcaatattt
     9661 gcttgaggac ctatatagtt aaaataattt tcctcttctt ttatataaaa gtcattgtgt
     9721 aaacgataag tctggctgtg ggtttccttg gcttttattt gaaagacttc ttcaacatcc
     9781 tgacgatcat tagcatatac gaatggaact ccttttttta aaacacctac tttttgttca
     9841 gctatatcta cataagttcg tcctaaaacc tcctgatgat ctaatccaat tgacggacaa
     9901 gtaaccgcta aagccttgaa catgttggta gaatcataat aaccgcccat tccagcctca
     9961 ataatgacga tatcaactgg acagctattg ccaaaataat agaacatcaa aactgttata
    10021 acttcaaatt ctgtagctgg ttctaggttg gtctcaaggt gcaaccgttc aacaactgga
    10081 cgaacagtct caacaagttt gataaaatca gactctggaa tcatttgacc atcaatgcta
    10141 atacgctctc taaagtcaac aatatatggt gatgtaaatg ttccaacttg ataaccagaa
    10201 tttgtaaaaa tatgttgtaa ataactagtt gttgatcctt taccattcgt tccaacaaca
    10261 tgaatggcag ataggttgtt ttgagggttt cctaactgct ctagcatcca tctcatacgt
    10321 tccaaacctg gtttaatacc aaaagctaat ttgctatgaa tccattctag cgcttcttga
    10381 taagtcatct tatttacctt tctttaacat cgtatgtact tgtccaatac taaaaactaa
    10441 aatagcaagc cagataaaaa taaaacctat tacctcccca ctaacgatag tttctttaaa
    10501 aataaacaat gctagcaata attggatggt tggattgatg tattggataa aaccgataat
    10561 atttagagga gccctcttaa tggcttctgc aaataataaa agaggaactg ctgtaatgat
    10621 tcctgaaagt gagagtaaaa ctaattgtaa tatattataa tcagttaaaa aatctttggc
    10681 gaaaaacact atataaatga gtgcaaacgg agcaataaaa ctactttcta ccagcatcga
    10741 aaaatcgcta gataaagaaa ttgatttttt taataaccca taaaaaccaa aagaaagcgc
    10801 tagagttagt gaaattaaag gaaaatgacc agtttgataa actaaaattc caactcccat
    10861 gattgcaatc aaaattgcaa ggctaactac tcttgccagg tgttcacgta aaacaaggac
    10921 cgacaacaaa attgaaataa tcggcatcat ataataacca agacttgcct cagtagcgtg
    10981 accatgcgtg actgcaaaaa tatagaccaa ccaattaatt gaaattaaaa tactggctac
    11041 aaacattgtc caaaatgact ttttattatt aactaagcct tttaagtctt ttaaatagac
    11101 tgtttttcta cccgaaacga gcatataaat aagcatagtt aaaacagtga atataatcct
    11161 ataagcaaac gtactatatg cttcaattcc agataataat ttccaataaa gagataaaag
    11221 cccccataaa acataggctg acaagccaag gataattcct aaattatctt ttctcaactc
    11281 tcactccttt tcgctcaata ttaagtactt gaatctgtcc atctaaacat aatttactta
    11341 actctgtgac aattggttgt tctttttctt tcgaacacag aaccattacc gtcggtcctg
    11401 ctccagacaa ataagtagca tacgcaccgt tttgagtgct gatttgtttt ataactccaa
    11461 attccttcac caaaggttga cgatatcttt catgaaagag gtccctttct atagcccatc
    11521 cagcagttac caaatcacct ttcaacaacg acgcaaccat cacattagcg acgctgcttg
    11581 aagcaactgc atcctcataa ctcaattttt gaggtagtac atttcgacta tcacttgttt
    11641 ttaattcata atttggaata aaagcgataa aggaactatc tggaaacatc acttccaaac
    11701 ttttaatatc atttttcact attgatgaaa taactaaatc accaaaaatg gccggtgcta
    11761 cattatcagg atgtccttct aatcgggtag ctatctctaa tttttctttt tgagaaagag
    11821 ccaaatttcc taattgattg gctaattcta ttcctgcaac aattacagag gaagaagaac
    11881 ctaaacctct ggccaaagga atatctgaaa acatttttaa gcggtgtggt gccaaatccg
    11941 acttaacatg caaagctgtt tgaataagga ggttagtatg atcttttgga atatttacta
    12001 aattgtgttc aactaaccac tctgtacttt cttctaatac ttcaatgctt aaatactttg
    12061 ataacgcaac tcctattgaa tcaaagcctg gaccaatatt cgcactagtt gcaggtacaa
    12121 ttatcctcat ttcaccctcc tattactttg aaaacattga ttaactgaat gacaccactc
    12181 tcaggaaact cctcaataaa ttgagttatt ttctcttggc taacttcatc tgttaataca
    12241 tagatttctt gttgtttatc atccaatgct ttttcaataa catttttcaa acctatattt
    12301 tcttgctcaa agtaccttcc aagtgtatca ctatcttcag catttctcaa agaaatagct
    12361 agatagtact cattgaagat atcagactgc attgacaacc tacacggaca atgataagta
    12421 ttcataggct taatttttat ctgtgatcga atactacgac taatatcgat gatatcggct
    12481 aaaacagagg ttgctgttgg attttgtccc gcacctggtc cataaaaaag agactgacca
    12541 atccctattg attcaatgaa tactgcattc atcacatgat taactgcagc taagggatgc
    12601 tcttgagaaa ttagtgtcgg agaaacgtct acacttatac cagttggagt ttcacgaaga
    12661 tcagttacca acttaatggc aaagcccatc tcctgagcta cttcaacgtc ctctgagcga
    12721 atactagaaa tccccttata attaacatca tcaaaatcaa taggcatacc aaatccaaat
    12781 tgacttaaga tagtggcttt gtaggcagta tcaatacctt caacatcatt tgtaggatca
    12841 ctttcggcat aacctaactc ttgtgccttt tttagagctt gttcataaga ccatccctct
    12901 tcaaacattt ttgttaacat gaagttggag gtaccgttga gaattcctaa taaacgtgtc
    12961 attttatctg aggcaaacga atgcgatagg gtccttaaaa taggaatgcc tccagcaact
    13021 gctccctcat agaataaagc tagacctcta gcgtctgcta aatccatcaa ctcttttccg
    13081 tgtaaagcaa tgagatcttt attggctgtc acaatatgtt taccaaatcc taatgcttga
    13141 ctcaaataag ttttagctgg ctcaatcccc cccattaatt ccactacaat atcaatttgt
    13201 ggatcttgaa gaatagtatt tatctctgtc acaaagttat aagtaaatcc ctgattgatg
    13261 aaacgctggc ggctttcatt atctcttact aatactttat caattacaat atcttcgcct
    13321 tctaaagaaa gtagcttatg ttgattttct tttagcaaat atggaatacc cttagcaacc
    13381 gttccaaaac ctaataaagc tattttaata gtcatgaatt tctccttaga aattgtctct
    13441 aacttcaaga caaacattgg ctatattata acaaattacc aatatttttt atagggtaaa
    13501 cgcgtcttgt cctatgaaag ttcttaaaaa gagaatatta tttcagaaaa gatgatacaa
    13561 taaaagtact ttaaaagtta aggagaaata aatggcacac acacctacta gtcatcgtaa
    13621 accacgaaaa cgttccccat ggttagctat tgcttctgtc ttttttttgt taatagcttt
    13681 aatcggcata tttctattct tcaacaatcg ttcaaagcaa gaaataaaaa caaaaacgaa
    13741 cgcctctagt catcgaaaaa ttgtcacatc tatcaaaaaa aagaaatggg ttaagcaaaa
    13801 gactcctgtt aaaataccca ttttaatgta ccatgctgtt catgttatgg acccttctga
    13861 agccgccagt gctaacttaa ttgtggcacc tgacattttt gaaagtcata taaaacgtct
    13921 caaaaaggaa ggatattact tcttagcccc taacgaggca tatcgtgcac taaacgaaaa
    13981 tgctttacct gaaaaaaaag tcatttggat tacttttgat gatggaaatg ccgatttcta
    14041 cactaaggca taccctatac tcaaaaagta caaggttaaa gccactaata atatcatcac
    14101 tggctttgtt caagaaggac gagaatctaa cctcaacgtt caacaaatgc tagaaatgaa
    14161 gcaaaacggc atgtcattcc aaggacatac ggttactcac cccaatcttt ctctcttaac
    14221 tccagaacta caaacccaag aaatgacact atcaaaactg tttttagatc aaaaactctc
    14281 tcaagatact cttgcgattg cttatccatc aggacgctac aatcctacaa ctttggatat
    14341 tgctagtcaa tattataaac tagggcttac aacaaacgaa ggagttgcaa caaaagataa
    14401 cggtttgctt tctcttaatc gagttcgtat cttaccaact actagcgatg atgatttgat
    14461 caaaacgatt aaccaataaa aagaagcctt ctggcttctt tttattcttc ataagattta
    14521 aatggttcat ttttacgtgg ataacgatct ggagaaatcg ataagcccat ttcttttttc
    14581 tcatggaaaa gctccactaa tagcgctagc atcatcaaaa tcattacgaa tgaaaatggc
    14641 agagcgacaa taattaaaac gttctgcagc gctaacagac caccagacaa gagtaagaca
    14701 atagcaatca ctgccaacac tagcccccaa attactttag ttcgattttt tggatttaaa
    14761 ttaccatctt ctgtcaacat agccaaaacg tacgttgcag aatctgctga tgtaataaaa
    14821 aatgaaaaaa ttaaaatgat agcaatagtg gataaaagcc aacctaaagt gtagtgatta
    14881 aaagtagcaa acaatacttc ctctgttgca aactttgtca aattaatacc cagttgttgt
    14941 acctgagttg ataatgtgcc aaatgctgca aaccatacaa aactcaataa tgtcggtatt
    15001 aaaagaacta ccgttaaaaa ctcccgaatg ctacgccctt tagaaatacg agcgatgaaa
    15061 actccaacaa aaggagacca ggaaatccac cacgcccaat aaaaaatcgt ccaattatca
    15121 atccaagaac gtttagtatt gtcaaaagca gctgcacgaa agctcattcc aaagaaattt
    15181 tgtaaataag agcctaaaga ctctgttagg gtatcaaaaa tacgaaccgt tggtcccaat
    15241 acaataacta aagctaagag ggctaccgct aaaatcaaat ttaagttcga taaaatttta
    15301 acaccttttc ctaaacctga taaagctgac ataacaaata aagctgttgt aataaggata
    15361 ataacaattt gaacaaatgc attattggga acacccaata aaaaacttaa ccctccgttg
    15421 atttgagcag ctccaaatcc aagtgtcgta gcaactccaa taactgtagc aacaactgtg
    15481 gtgatatcaa caatttttcc tagtcaacca tctgtcttat caccgaaaag aggctttaaa
    15541 gtgacagaca agaggtattt ctcttgcttt cgaaatccaa aataagctag agccaaagca
    15601 accaatgcat atactgccca agcatgtatc ccccagtgaa aaaatgtaaa acgaaatgca
    15661 tcagctaatg ctgtttgcga ttccttaggt gcgccaggtg tcgaaatagc aaaatgcgat
    15721 aaggggtcag ctgctccgta gaagaccaaa ccaatcccca tacctgctga aaacatcatt
    15781 gctatccatg atacagttga atattcagga cgtgaatgag gtcgccctaa acgtatttgt
    15841 ccaagaggac tcataataaa aaagacacac aaagcaagaa ttgaagtaac taacaacaag
    15901 tacaaccaac cgaaattggt agatacctgt tcacgcaaga tttgtgtcca agattgaaaa
    15961 ccacgaggaa caataattcc taataagact aaaataagcg atacaattag tgaacctgta
    16021 aacacaggcg taatatgttt tttactcaaa atttcctcct atactaaaat agtatagcat
    16081 actttaataa atactcctct cattttaatc acctgtatca agacctctcc ttgtctcctc
    16141 aacaaaaaag aaccagataa cattatctgg ttcttgtgtt tgacttagtg attaatcact
    16201 atttggtgta aataccaaat gttcgttgac aaaggtatca aatccaagtt ggctcaattc
    16261 acgaccatat cctgaatttt taatacctcc aaacggtaat tccggtaatg atgtccaccc
    16321 tgaattaata aatgtcatcc ctgtttcaat ctgagccgcc acttttttag catgttctgg
    16381 atcagaagag aaaacagtac ttcctaaacc ataactagaa tcattagcta aagcaatagc
    16441 ttcttcttca gtatccactt tatagataga ggctacgggg ccgaaaattt cttgattata
    16501 gattggattt gctttagtga tattcgttaa aacagttggc atgacgaagt ttcctggatg
    16561 atctatagtg tcatttccaa aaactacctc agcaccgtgg tctaccgcca atttaatttg
    16621 ttttaaaaca tcatcctttg cacctgcaga tgacagtgga gccaaggttg tttccgaatc
    16681 cattgggtca ccccatttag cagacttaaa ggtctcaact accatattta caaattcgtc
    16741 atacttatca gccataacaa tgaaacgttt tgaagatgta catacttgac ctgcattata
    16801 taaacgcgca aagaatattg ttttactaag taaatcaaaa tcagcatcat ctaaaataag
    16861 aaaagcatcg ttcccaccta attccataga cgattttttc aaatttttac cagcctcagc
    16921 tgctattgat gccccaccac gctcagaacc agttaaacac acacctgcaa cacgaggatc
    16981 tgatataagg tttgaaactt gatcatacga agcaaaaata tttttaaacg ctccttctgg
    17041 agctcctgct tctcggacta aatcctcaaa tgcttgagct gatgcaggac aaatagaagc
    17101 atgttttaaa agcatagtat tacccactat aaagttaggg gcaaatacac gcataatctg
    17161 ataaaatggg aaattccatg gctcgacagc aagtaaaaca ccgacagctt gtttcaaata
    17221 gtaagcctct ccatttggac tttctaacgg aacaggtttt aaaaactttt gcccattatc
    17281 agcataatag tcagctatgt ctgcacacaa gtccacttct ccttgtgctt cagtgaaaag
    17341 ttttcccata tctttagtca taacttcagc gtacttatca cggtccttac gtaataaatc
    17401 tgctacttta tgcaattgat tttggcgttc ttcaacatta tcttctttgc gccatgtctt
    17461 gtagagggca tgcgctatgt ctaatgactg ttcgagatca ctgtcactga tattatcgaa
    17521 ttcatgcaat acttcgttag tgtacggata aattgtttta tatgccataa tgttccctcc
    17581 ttttgatatc ctcattataa catttatgaa atcgattaca aatatttagc tttagacaag
    17641 atcaccaagt tccaaggata gcttgcattg ttaaactaac tataataatg cctaaccaac
    17701 acgctgcacc taaaagaatt gattttcctc cagttttaat tagtttgctg acattagtat
    17761 ttataccaat tgcccccatt gccataacaa taaaaaattt agataaaacc ttaagtggct
    17821 caaagatacg taacgaaaaa cctaatgaag caactatcgt tgttagcaaa gaagctaaga
    17881 taaaataaag cacaaacttc ggaaatatct tagtaacact tacagattgt tctttctgcg
    17941 tttgtttcat atgataaata gataatacaa tcgtaattgg aatgatggct aatgttcgtg
    18001 tcaatttaac aatagtagct ccgcccaaag tattgctgtg attgatagca tcccatgcag
    18061 tagcagttgc tgtaactgac gaggtatcat tgacagcagt ccctgcaaat aatgcaaaac
    18121 cgtgatcaga gagaccaata aaattcccca gcgttggaaa aattaaagcc gctaagatat
    18181 tgaataaaaa aataaccgaa atagcttgtg ctacttcatc atctttagca ttaataactg
    18241 gtgcagtcgc tgcaattgcg gatccaccac aaatagatga accaacacct attaaagtag
    18301 caatcgtttt atctaactta aataactttt gcaaaacata agcaataatc aatgaaatgg
    18361 aaatcgtcat aataatgatg ggtaacgatg atataccaac tttcatcact tgcatgaggt
    18421 ttagtccaaa acccaataag acaactgctg tttgtaaaat atatttagac gtaaaagcaa
    18481 tacccttatt aaacaattgt cgattttgat aaaaggatcc aacaattatt ccaatcaaga
    18541 tagcaaagac tggtgctcct attaaaggga gataaagccc aagcaaccaa gatggtatag
    18601 caataataaa gcataatatt agtccaggaa ttttttcctt aaataacatg atagtcctcc
    18661 tcacattgac tattatatca caaactttaa aacgtggcag gaaaaccgaa agaggaccag
    18721 aatatgctct gatcctcttt cattactaat tcacaattat ttagttactt tcataaatgg
    18781 atatgatttt tcgttaccta aaatcaattt tgagccctct tttttatagg ctgttttttc
    18841 accatttttc acaatattac catagaacgc atttgccttt ttattgatat gcgatacttt
    18901 aataacactt gttaagcgat gtactttacc atccataact ggcaatgtca tttcaccagt
    18961 attagtatta tattttcctc cgacagcttg tgcagcactt tggaaacttc tatcaatttg
    19021 ttggtcaatt tgtttgtcag tgatttttgt ttcatcaaaa atctttttac ctgcttcact
    19081 taaatcattg taagtcaaac cttgatcttt taattgtttt tcaagagctt tatcaataaa
    19141 agtttttata gcttgatgga attttacctc atcaatttga gcagtgactg ttatctttgc
    19201 ctcatcgttt ttgacgttca tatgcatatt agcgccctta accaaatcag ctgcactaat
    19261 ccccatatcc gctgcctgac ttccaagttg ttgttctatt tctttctcaa cttgcttctc
    19321 taaagaaact gtacgccatt tcccctctag tgactgacga ttattccaga aatagagacc
    19381 gcctgcacct acaattagaa taagtactag tacagttaaa aaaactttta aaaacttttt
    19441 catgataa
//
LOCUS       DATROD010000024        19417 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.024, whole genome shotgun sequence.
ACCESSION   DATROD010000024 DATROD010000000
VERSION     DATROD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19417
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.024"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            6..731
                     /locus_tag="VBC74_000863"
     CDS             6..731
                     /locus_tag="VBC74_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6941827.1"
                     /translation="MLKIENVTGGYVNIPVLKNISFEVNDGELVGLIGLNGAGKSTTI
                     NEIIGILRPYQGDITIDGISLEANQELYRKKIGFIPETPSLYEELTLREHLETVAMAY
                     DIATDEVMARAQKLLEMFRLTDKLDWFPMHFSKGMKQKVMIICAFVVSPSLFIVDEPF
                     LGLDPLAISDLINLLAEEKAKGKSILMSTHVLDSAEKMCDRFVILHKGEIRAVGTLEE
                     LRAIFGDSNANLNDIYIALTKEV"
     gene            733..1767
                     /locus_tag="VBC74_000864"
     CDS             733..1767
                     /locus_tag="VBC74_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6941828.1"
                     /translation="MKKLFNKRRSLFLTQNSKYLRYVFNDHFVLVLMFLSGFLLYQYS
                     QLLKDFPKTHWPIIVIVSIIILMLLAMGEIASYLEPADKQFLLIKEEAIKEIINSAKK
                     RTYIFWLVVQTLFLVLISPILIKLGLSVFMITLLIFGLGIIKWLVITYKVKVFYNNQN
                     LNWDAAINHEQERKQAILKFFSLFTNVKGISTSVKRRSFLDVILKLISKTPSRLWTNL
                     FVRAFLRSSDYLGLTIRLVTLNILSVIFVNETYLALALAFVFNYLLLFQLLALGHHFD
                     YQYMNQLYPVRLNAKASQLKGFLRVLSYAVTVIDIILIRELKPVILLIVLMLIVTEYY
                     IPYKIKKMID"
     gene            1825..2625
                     /locus_tag="VBC74_000865"
     CDS             1825..2625
                     /locus_tag="VBC74_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEO6941829.1"
                     /translation="MTISNQELTLTPLRGKSGKAYIGTYPNGERVFVKYNTTPILPAL
                     AKEQIAPQLLWARRTSNGDMMSAQEWLDGRTLTKEDMGSKQIIHILLRLHKSRPLVNQ
                     LLQLGYKIENPYDLLMDWEKQTPIQIRENTYLQSIVTELKRSLPEFRTEVATIVHGDI
                     KHSNWVITTSGLIYLVDWDSVRLTDRMYDVAYILSHYIPQKHWKDWLSYYGYKDNEKV
                     WSKIIWYGQFSYLSQIIKCFDKRDMEHVNQEIYELRKFRELIKKYNAS"
     gene            2615..3250
                     /gene="trmB"
                     /locus_tag="VBC74_000866"
     CDS             2615..3250
                     /gene="trmB"
                     /locus_tag="VBC74_000866"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEO6941830.1"
                     /translation="MRVRKRKGAEEHLENNPHYVISNPEEAKGRWHEIFGNNNPIHIE
                     VGSGKGAFITGMAEQNPDINYIGIDIQLSVLSYALDKVLDSGAKNIKLLLVDGSSLSN
                     YFDTGEVDLMYLNFSDPWPKKKHEKRRLTYKTFLDTYKDILPEQGEIHFKTDNRGLFE
                     YSLASFSQYGMTLKQVWLDLHASDYQQNIMTEYERKFSNKGQVIYRVEARF"
     gene            3294..3381
                     /locus_tag="VBC74_000867"
     tRNA            3294..3381
                     /locus_tag="VBC74_000867"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3328..3330,aa:Ser,seq:gga)
     gene            3739..4215
                     /gene="rimP"
                     /locus_tag="VBC74_000868"
     CDS             3739..4215
                     /gene="rimP"
                     /locus_tag="VBC74_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEO6941831.1"
                     /translation="MANQTIVDIVTQVVTPAIQAPFELVDVEYEKMGGDYVLSILIDK
                     PGGITVEDTAQLTDVVSPLLDTIQPDPFPEQYMLEVSSPGLERPLKTAEALSNAVGSY
                     INVSLYKSIDKVKIFEGDLLSFDGETLTIDYMDKTRHKTVDIPYQTVAKARLAVKL"
     gene            4242..5402
                     /gene="nusA"
                     /locus_tag="VBC74_000869"
     CDS             4242..5402
                     /gene="nusA"
                     /locus_tag="VBC74_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEO6941832.1"
                     /translation="MDTMSKEMLEAFRILEEEKHINKEDIIDAVTESLKSAYKRRYGQ
                     SESCVIEFNEKKADFTVYTVREVVDEVFDSRLEISLKDALAISSAYELGDKIRFEESV
                     TEFGRVAAQSAKQTIMEKMRRQMREVTFNEYKQHEGEIMTGTVERFDQRFIYVNLGSL
                     EAQLSHQDQIPGESFKSHDMIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEREIPEV
                     FDGTVEIMSVSREAGDRTKVAVRSHNSNVDAIGTIVGRGGSNIKKVISNFHPKRVDAK
                     TGLEIPVEENIDVIQWVEDPAEFIYNAIAPAEVDMVLFDDEDTKRATVVVPDSKLSLA
                     IGRRGQNVRLAAHLTGYRIDIKSASEYEKMEAQELQTEEVAQESEVISDKAE"
     gene            5424..5720
                     /locus_tag="VBC74_000870"
     CDS             5424..5720
                     /locus_tag="VBC74_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEO6941833.1"
                     /translation="MAKTKKIPLRKSVVSGEVIDKRDLLRIVKNKEGQVFIDPTGKQN
                     GRGAYIKLDNDEAILAKKKRVFDRSFSMEVSDEFYDELLAYVDHKVKRRELGLE"
     gene            5713..6015
                     /locus_tag="VBC74_000871"
     CDS             5713..6015
                     /locus_tag="VBC74_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEO6941834.1"
                     /translation="MNNSEKVLNLIGLAQRAGRLITGEELVIKAIQNQQVSLIFLAND
                     AGPNLTKKVTDKSNYYKTEVSTVFSTLELSDALGKPRKVVAVADAGFSKKMRTLMA"
     gene            6035..8818
                     /gene="infB"
                     /locus_tag="VBC74_000872"
     CDS             6035..8818
                     /gene="infB"
                     /locus_tag="VBC74_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEO6941835.1"
                     /translation="MSKKRLHEIAKEIGKTSKEVVEQAQSLGLPVKSHASSVEENDAT
                     RIVESFSSSKTKAPTNSVQTNQGVKTESKTVETKQGLSDDKPSTQPVAKPKPQSRNFK
                     AEREARAKAEAEKRQHNGDHRKNNRHNDTRSDDRRHQGQKRSNGNRNDNRQGQQNNRN
                     KNDGRYADYKQKPQTRPQQPAGNRIDFKARAAALKAEQNAEYSRHSEQRFREEQEAKR
                     QAAKEQELAKAAALKAQEEAQKAKEKLASKPVAKVKEIVNKVAATPSQTADSRRKKQT
                     RSDKSRQFSNENEDGQKQTKNKKNWNNQNQVRNQRNSNWNHNKKNKKGKTNGAPKPVT
                     ERKFHELPKEFEYTEGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGDTIELL
                     MVDYGIEAHAKVEVDEADIERFFADEDYLNPDNLTERPPVVTIMGHVDHGKTTLLDTL
                     RNSRVATGEAGGITQHIGAYQIEEAGKKITFLDTPGHAAFTSMRARGASVTDITILIV
                     AADDGVMPQTVEAINHSKAAGVPIIVAINKIDKPGANPERVISELAEHGVISTAWGGE
                     SEFVEISAKFGKNIQELLETVLLVAEMEELKADADVRAIGTVIEARLDKGKGAVATLL
                     VQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGDHFAV
                     YADEKAARAAGEERAKRALLKQRQNTQRVSLENLFDTLKAGEVKSVNVIIKADVQGSV
                     EALAASLLKIDVEGVKVNVVHSAVGAINESDVTLAEASNAVIIGFNVRPTPQARQQAD
                     ADDVEIRQHSIIYKVIEEVEEAMKGKLDPEYQEKILGEAIIRETFKVSKVGTIGGFMV
                     INGKVTRDSSVRVIRDGVVIFDGKLASLKHYKDDVKEVGNAQEGGLMIENYNDLKEDD
                     TIEAYIMEEIKRK"
     gene            8908..9276
                     /gene="rbfA"
                     /locus_tag="VBC74_000873"
     CDS             8908..9276
                     /gene="rbfA"
                     /locus_tag="VBC74_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEO6941836.1"
                     /translation="MKKGLIMANHRIDRVGMEIKREVNEILRLRVNDPRVQDVTITDV
                     QMLGDLSMAKVFYTIHSTLASDNQKAQIGLEKATGTIKRELGKNLTMYKIPDLQFVKD
                     ESIEYGNKIDEMLRNLDKKD"
     gene            complement(9348..10352)
                     /locus_tag="VBC74_000874"
     CDS             complement(9348..10352)
                     /locus_tag="VBC74_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6941837.1"
                     /translation="MKPSFKKLLLLFSIITILSIACTPHAKASGRSWKSWFIEQYFWL
                     KRDKSYYKVQDESSFQKYLNASREQSDKGYYLDPNSVNGGLVQERLFDMQVYSWNDNG
                     KANQKTIFYLAGGSYLNNPTPYHISMLKTLSTSLDAKIILPIYPKTPRYTYDYAIPRL
                     VNLYRHFHEKNANLTLMGDSAGGGLALGLAHALSHQSGQEAIPQPKNIILLSPWLDVT
                     MKHPEIPKYEDTDPILSAWGLARVGEIWANGSNNTNHTYVSPKNAPATKLAPITLFTG
                     TREIFFPDIRDYAAQLQAANHPVNYIAQEGMNHVYPIYPIEEAKTAQYQMIDIINKTP
                     "
     gene            10515..10931
                     /locus_tag="VBC74_000875"
     CDS             10515..10931
                     /locus_tag="VBC74_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEO6941838.1"
                     /translation="MTISNAEWEIMRVVWAQQNTTSNEILAVLLEKYDWTPSTVKTLL
                     RRLLDKGYVSREKMGKGFSYSSLIDEDLAMMSEVDSVLQKVCQTKHVAIVRHLLESIP
                     MTEKDRLNLQASLEAKKGKTLERVACNCIPGQCQCH"
     gene            10944..13178
                     /locus_tag="VBC74_000876"
     CDS             10944..13178
                     /locus_tag="VBC74_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEO6941839.1"
                     /translation="MANRKETFLIDGMTCASCALTIEKAVNKLDHVDSAVVNLATEKM
                     TVTFDDTTLSPNVIEECVSETGYEASLFKEETSKSQSERHQLAIEKMWHRFWMSAVAT
                     IPLLYISMEPMINLWLPSFLMPDKGPLNYGVIQLLLTLPVMYFGRIFYQNGFKALFKR
                     HPNMDSLVAIATTAAFIYSLYGLYEILQGDIHYAHQLYFESVAVILTLITLGKYFEIL
                     SKGRTSASIEKLLTLSAKEARVIKDGEDYMVPLDKVKIGETILVKPGEKIPLDGHVVA
                     GESSIDESMLTGESIPVEKKVGSKVYGASINGQGSLTIFVEKEAVGSLLSQIINLVEV
                     AQASKAPIANLADKVSGVFVPFVIVIAILSGLSWYLILGQSFAFSLKIMIAVLVIACP
                     CALGLATPTAIMVASGKAAENGILFKGGEVLEKAHHIDTIVFDKTGTLTKGKPEVVAI
                     KTYGVDKEEFLGQVASVEKLSNHPLSQTIVNKAKEKELPLREVMAFKNILGYGLSAII
                     NGKTMLVGNANLMTKNDVNLDLAKADIEIAQEEAQTVVYVSENGVLSGLITLTDQLKT
                     DSQETVKQLQRLGFNLVLLTGDNKASADAIAQKLGITTVVSEVLPDQKANVILELKEK
                     GGQIAMVGDGINDAPALASSDVGISMSSGTDIAIESADIVLMKPELTDLLKAMTISKQ
                     TIQIIKENLFWAFFYNVLAIPVAMGVLHLFGGPLLNPMLAGLAMAFSSVSVVLNALRL
                     KVLK"
     gene            13219..13425
                     /locus_tag="VBC74_000877"
     CDS             13219..13425
                     /locus_tag="VBC74_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEO6941840.1"
                     /translation="MKHTYRVSGMKCDGCAKTVSDKLSSVMGVDEVNVDLTKNQVVVS
                     GKTFKCLLKRSLKDTKYSLEEEIN"
     gene            13535..14149
                     /locus_tag="VBC74_000878"
     CDS             13535..14149
                     /locus_tag="VBC74_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEO6941841.1"
                     /translation="MSIDIWNILSLVGTVAFASSGAIVAIEEEFDVLGLFILGFVTAF
                     GGGAIRNVLIGLPIETLWSQGIAFYAAAAAILFIMIFPNLLSGKGRDAEVVSDAIGLA
                     AFSVQGALYATQSHQPLSAVIVAAVLTGAGGGIVRDVLAGRKPGVLRSEIYAGWSILV
                     GIILYFKIAKTTTDYYLLVLVVTSLRMLGYKKQWHLPVVRPRKK"
     gene            14164..14976
                     /locus_tag="VBC74_000879"
     CDS             14164..14976
                     /locus_tag="VBC74_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6941842.1"
                     /translation="MIKLIAIDMDGTLLNDEKKIPKENIQAIKEATQAGIKIVLCTGR
                     PMSGILPYFNELGLTKEEYIIMNNGCSTYSTKDWQLIDSATLTHDELIFLEEVVKEFP
                     NVCLTLTAENTFYAVGEEVPEIVAYDADLVFTKAKSISLDALRNQEEIVFQAMYMGLD
                     ADVTAFQEAVEEALISKFSGVRSQDYIYEIMPQGVTKARGLKSLIAKLGLDINQVMAI
                     GDAPNDIELLDLVPNSVAMGNASDEIKSRCKYITVDNNQAGVAKAIYDYALR"
     gene            15089..17731
                     /gene="polA"
                     /locus_tag="VBC74_000880"
     CDS             15089..17731
                     /gene="polA"
                     /locus_tag="VBC74_000880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEO6941843.1"
                     /translation="MTNKNKLLLIDGSSVAFRAFFALYNQIDRFKNNSGLHTNAIYGF
                     HLMLNHILGRVQPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPYIRQQLD
                     VLGIKHYELEHYEADDIIGTLAKQAEASKEHFDITVVSGDKDLIQLTDTNTVVEISKK
                     GVAEFEEFTPAYLMEKMGITPSQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLSEYGS
                     LEGIYENIEAMKQSKMKENLINDKEQAFLSKTLATINIASPITIGLEDILYSGPQDIK
                     ALSQFYDEMDFKQFKAALGEETSQEDFEVDFTEVEQLKPEMFSDNDFYYFEMLGDNYH
                     VEDLIGIAWGNSDTIYATSDVSLLQEALFKKALSKPIKTYDFKRSKVLLNRFNIDLPE
                     PAFDTRLAKYLLSTTEDNLVSTIARLYTNLPLDTDDAVYGKGAKRAIPEKTRFLEHLA
                     KKVKVLVDSEANIMQQLKANEQEELLFEMEQPLANVLAKMEIRGIKVKKNTLNEMAIE
                     NQKVIETLTQEIYELAGQEFNINSPKQLGKLLFETLGLPVEMTKKTKTGYSTAVDVLE
                     RLAPISPLVTKILEYRQITKLQSTYIIGLQDYILEDGKIHTRYVQDLTQTGRLSSSDP
                     NLQNIPVRLEQGRLIRKAFVPSEDNAVLLSSDYSQIELRVLAHISKDEHLIAAFKEGA
                     DIHTSTAMRVFGIEKPENVTPNDRRNAKAVNFGIVYGISDFGLSHNLGIPRKLAKQYI
                     DTYFERYPGIKNYMETVVREAKDKGYVETLFHRRRSLPDINSRNFNIRQFAERTAINS
                     PIQGSAADILKIAMINLDRVLDKGGYKSKMLLQVHDEIVLEVPNEEIGAIKELVTKTM
                     ESAISLSVPLIADENAGETWYEAK"
     gene            17761..18201
                     /locus_tag="VBC74_000881"
     CDS             17761..18201
                     /locus_tag="VBC74_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEO6941844.1"
                     /translation="MVYHFQNLSDFMLKNYLTKAKTIAVVGLSDRQETAAYQVSKIMQ
                     EAGYQIIPVNPKNAGQKILGQMTYASLKDVTEHIDIVNIFRRSEYLPDIAREFLEVDA
                     DIFWAQLGLESQEAETILKQAGHKQIVMNKCLKVEYQKLHSETN"
     gene            18283..18762
                     /gene="perR"
                     /locus_tag="VBC74_000882"
     CDS             18283..18762
                     /gene="perR"
                     /locus_tag="VBC74_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEO6941845.1"
                     /translation="MDSHSHGHRPLDAYENVLEHLREKRIRITETRKAIISYMVNSRE
                     HPSAEKIYNDLLPEYPNMSLATVYNNLKVLVDEGFVTELKLCNYSTTYYDFMGHQHLN
                     IACEDCGKIVDFVDVDLLDISREAHQQTGFEVTRVQLVAYGICPECQRKRKDKYKKD"
     gene            18915..>19417
                     /locus_tag="VBC74_000883"
     CDS             18915..>19417
                     /locus_tag="VBC74_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703861.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEO6941846.1"
                     /translation="MKISQYNKWSIRRLKVGAASVMIASGSIVALGQSHIVSADEMSQ
                     PKTTITAPTANTSTNVESSTDKALSKVTTMETSSEMPKMQNMAKVEKTSDKPMMVATS
                     VRKMMATPTPVAMTKTTSVDEVKKSTDTAFKQTVDVPAHYVNAAKGNGPFLAGVNQTI
                     PYEAFGG"
BASE COUNT         6537 a         2980 c         4005 g         5895 t
ORIGIN      
        1 tgacaatgtt aaaaattgaa aatgttacag gggggtatgt taatatccca gttttaaaaa
       61 atatttcttt tgaagtaaat gatggagagt tagttggatt aattgggctt aatggtgcag
      121 gaaaatcaac cactattaac gagattattg gtattttgag accctatcaa ggagatatta
      181 ccattgatgg cattagtctt gaagccaacc aagaacttta tagaaagaaa ataggcttta
      241 ttcctgagac accaagtctc tatgaggaac taactctcag agaacattta gaaacggtag
      301 ctatggcgta tgatattgcc accgatgaag ttatggcacg tgcgcaaaaa ctattagaga
      361 tgtttcgtct aacagataaa ttagactggt ttccaatgca tttttcaaag ggaatgaaac
      421 aaaaggttat gattatctgt gcttttgttg tatctccaag tttatttatt gttgatgaac
      481 catttttagg tttggatcca ctagctattt cagatttgat taatcttcta gcagaagaaa
      541 aagctaaagg gaaatctatt ttgatgtcaa cacacgtatt agattctgcc gaaaaaatgt
      601 gtgatcgttt tgttatttta cataaaggag aaatacgggc agttggaacc ctagaggaat
      661 tacgggctat ttttggagac agtaatgcta atttaaatga catctacata gctctgacaa
      721 aagaggtgtg atatgaaaaa gttatttaat aaacgtcgaa gtctcttttt aactcaaaat
      781 agtaaatatt tgagatacgt ctttaatgat cattttgtat tagtattaat gtttttgtca
      841 ggttttttgt tatatcagta tagccagtta ctaaaagact ttccaaaaac acattggcca
      901 attatcgtga ttgtgtcaat cattatctta atgttactag cgatggggga aatagccagc
      961 tatttagaac cggctgataa acaattttta cttataaaag aagaagcaat aaaggaaatt
     1021 ataaattctg ctaaaaaacg gacttatatc ttttggttgg ttgttcaaac cttattttta
     1081 gtattaatat caccaatttt gataaagtta ggcttgtcag tttttatgat tacattgtta
     1141 atttttggtc taggaataat caaatggttg gtgataactt acaaggtaaa ggtcttttat
     1201 aacaatcaaa atttgaactg ggatgcagct ataaatcatg agcaggaaag aaaacaggct
     1261 atattaaaat ttttctcttt gtttacaaat gttaagggaa tttcgacaag tgtaaaacgt
     1321 cgttcatttt tagatgttat tttaaaactt atttctaaaa cgccatcgcg tttatggact
     1381 aacttatttg tgagggcatt tctaagaagc tcagattatc ttggtttaac cataaggttg
     1441 gtaactttaa atatcttatc agtcatcttt gttaacgaaa cctacctcgc ccttgctctg
     1501 gcatttgtat ttaactactt attattgttc caattattag ctttaggtca ccattttgat
     1561 tatcaataca tgaatcagct ctatccagtt agattaaatg ctaaagcaag tcagctaaaa
     1621 ggttttctca gagtgttatc atatgctgtg acagtaattg atatcatact tataagagaa
     1681 ttgaaacctg ttatattatt gatagttttg atgttaattg tgacggaata ttacatccct
     1741 tacaaaataa agaagatgat tgactaaagc ctataaaaca agtaaaataa gaggaaacga
     1801 ctaaatgaaa aggagggact gctggtgaca atatcaaatc aagaattgac gcttactccc
     1861 ttaagaggaa aaagtggcaa agcctatatt ggtacttatc caaatggtga acgtgtcttt
     1921 gtcaaatata atacgacacc tattttgcca gccttagcta aggaacagat tgcgccacaa
     1981 ctactgtggg cacgccgtac aagtaatggc gatatgatga gtgctcaaga atggttagat
     2041 ggtcgaacat tgactaaaga agatatgggt agtaaacaga ttattcatat tttactgcgc
     2101 ctgcacaagt ctcgtccctt agtaaatcaa ctgttacaat tagggtataa aattgaaaac
     2161 ccttatgatt tgttgatgga ttgggaaaaa caaactccta ttcaaatccg agaaaacact
     2221 tatttgcaat ctattgtgac tgagttaaag cgtagtttac ctgagtttcg aacagaggtt
     2281 gcaacaatcg ttcacggaga tatcaaacat agcaactggg ttattacaac aagtggttta
     2341 atttatttgg ttgattggga ttctgttcgt ttaacagatc gcatgtatga cgttgcatat
     2401 attttaagtc attatattcc acaaaaacac tggaaagatt ggctgtctta ctacggttat
     2461 aaagataatg agaaagtctg gagcaaaatt atttggtatg ggcaattttc atatttatca
     2521 cagataatca aatgttttga taaacgagat atggagcatg tcaatcaaga aatttatgaa
     2581 ttacgaaagt ttagagagtt aattaaaaag tataatgcga gttagaaaac gtaagggtgc
     2641 agaggaacac ttagaaaata atccgcacta tgttatttca aatccagaag aagccaaagg
     2701 gcgttggcat gagatttttg gaaataataa ccccattcac atagaagttg gttccggaaa
     2761 aggggctttt atcactggta tggcagagca aaatccagat atcaattata ttggaattga
     2821 tattcaattg tcagtgcttt cctatgctct ggataaagtt ctagatagcg gtgctaagaa
     2881 tatcaaatta ctattagttg atggttctag tctatcaaac tactttgata ctggcgaagt
     2941 tgatttgatg tatcttaact ttagtgatcc atggcctaag aaaaagcatg agaaacgtcg
     3001 attgacctat aagactttct tggataccta taaagacata cttcctgagc agggggaaat
     3061 tcattttaaa acagataata gaggtttatt tgaatatagc cttgctagct tttctcaata
     3121 cggtatgact ttaaaacaag tttggttaga tttacatgca agtgattacc agcaaaatat
     3181 catgactgag tacgaacgca aattttcaaa taaaggacag gtcatctatc gtgtagaagc
     3241 acgattttag aataagtaaa tagtaagtgg gtagttattt gctactcctt tatggagagg
     3301 tcgcatagtg gctgagtgcg cacgcttgga aagcgtgtag tccctaatgg gggctcgggg
     3361 gttcgaatcc cctcctctcc tttaatcttg ttaatgtatc agtgtttatt aagtgttcac
     3421 ctttttcact cgtcaaaagt ccaagtttcg aaaaatttct tttattttat catcgtttct
     3481 ctttgagctt gaagtattag cataaagttt tctaagctct tacaaagaat tgaggacaaa
     3541 ttttaatttt ttgatataag attagtgtac ggtgatgcta atttaaagta aatgattgca
     3601 ataatcgtat tttgtgctat aatagtatta aataatacag aaaggggcgg agttttactt
     3661 cgcctcttat gtgtgtgatt catagaattt atagtcattg taagttctaa agcaagttag
     3721 aaagaagagg tagctgttat cgcaaatcaa accattgttg atattgttac tcaagtcgta
     3781 actcctgcta ttcaagcacc gtttgaatta gtagatgtag aatacgaaaa aatgggtggc
     3841 gactatgtgc tcagtatttt aattgataaa cccggaggca ttacggttga agatacagca
     3901 caattgacag acgttgtaag tcccttactt gacacaattc aaccagatcc atttccagaa
     3961 cagtatatgc ttgaagtctc aagtcctggg ttggaaaggc cacttaaaac agcagaagca
     4021 ttatcaaatg ctgttgggtc ttatatcaat gtaagtctgt ataaatccat tgataaagtt
     4081 aaaatatttg aaggtgactt attatcattt gatggtgaaa ccttgacaat tgattatatg
     4141 gacaaaacac gtcataaaac tgtagatatt ccctatcaga cggttgcaaa agcccgtctt
     4201 gcggttaaac tttaagaaag gatgattctt gcgaagcaag aatggacact atgagcaaag
     4261 aaatgctaga agccttccgt attttggaag aagaaaaaca cattaataaa gaagatatta
     4321 ttgatgcagt tactgagtca ctaaaatctg cctataagcg tcgttatggt caatcagaat
     4381 catgcgttat tgaatttaac gagaagaaag cagactttac cgtctatact gttcgtgaag
     4441 ttgttgatga agtttttgat agccgtttgg aaattagcct aaaagacgca cttgctatta
     4501 gctcagcgta tgaacttggt gataagattc gctttgagga atctgttaca gaatttggac
     4561 gcgtggcagc tcagtcagca aaacaaacta ttatggaaaa aatgcgtcgt caaatgcgtg
     4621 aagtaacctt caatgaatac aaacaacacg aaggtgaaat tatgactggt actgttgaac
     4681 gatttgacca acgttttatc tatgtcaatc tcggttcact cgaggcgcaa ttatcgcatc
     4741 aagatcagat tcctggggag agctttaaat ctcacgatat gattgacgtt tatgtctata
     4801 aagtggagaa taatcctaaa ggtgtcaacg tttttgttag ccgtagtcat ccagagttta
     4861 tcaaacgcat tatggagcga gaaattccag aagtatttga tggaactgtt gagattatga
     4921 gcgtctctcg tgaggctgga gatcgtacta aagttgctgt acgcagtcat aattcaaacg
     4981 ttgatgcgat tggaacaatt gttggacgcg gtggaagtaa cattaaaaaa gttatcagta
     5041 atttccatcc taaacgcgtt gatgctaaaa caggattgga gatcccagtt gaagaaaaca
     5101 ttgatgttat tcaatgggtt gaagatccag cggaattcat ttacaatgct attgcaccag
     5161 ccgaagttga tatggtactt tttgatgatg aagatacaaa acgtgctact gtagttgttc
     5221 cagatagcaa gttatcgcta gctattggtc gccgaggaca aaatgtgcgt ttagcagctc
     5281 atttgactgg ctatcgtatt gatattaaat ctgcaagtga atacgaaaaa atggaagcgc
     5341 aagaacttca aactgaggaa gtagctcaag aatctgaagt tatttcagat aaggcagaat
     5401 aatataaatg agagaggtgt tttatggcta aaacaaaaaa aataccttta agaaaatcag
     5461 ttgtttctgg tgaagtgatc gataagcgag atcttttacg aattgttaaa aataaagaag
     5521 gacaggtttt tatagatcct accggaaagc aaaatggtcg aggtgcctac ataaaattgg
     5581 acaatgatga agctatccta gcgaaaaaga aacgagtgtt tgatcgcagt ttttcaatgg
     5641 aagtttctga tgaattctat gatgaactat tggcatatgt cgaccataag gtcaaaagaa
     5701 gagagttagg ccttgaataa ttcagaaaaa gtattaaatc tcattgggtt agcacagcgt
     5761 gcggggcgcc ttattacagg tgaagaattg gttattaaag caattcaaaa tcaacaagta
     5821 tcgctaatct ttctagcaaa tgatgctggc ccaaatttga cgaaaaaagt aactgataaa
     5881 agtaattact acaaaacaga agtctccaca gtgttttcaa cactggaatt aagtgatgcg
     5941 ttaggcaaac caagaaaagt ggttgctgtc gcagacgctg gattttcaaa gaaaatgagg
     6001 actcttatgg cataacagaa taggaggaca tcaattgtca aagaaaagat tacacgaaat
     6061 cgctaaggaa attggaaaaa cgagtaagga agtggttgag caagctcaat cgcttggctt
     6121 acctgtaaaa agtcatgctt ctagcgttga agaaaatgat gctacacgta ttgttgagag
     6181 ttttagctct tctaaaacta aagctccaac aaattctgtt caaactaatc aaggtgtgaa
     6241 aacagaatct aaaactgttg aaaccaaaca aggcttgtcc gatgataaac cttcaactca
     6301 acctgtagct aaaccgaaac cacaaagtcg taactttaag gcggagcgcg aggctcgtgc
     6361 taaagctgaa gcggagaagc gtcaacataa tggggatcac cgtaagaata atcgccataa
     6421 tgatacacgc tctgacgaca gacgccatca aggtcaaaag cgttcaaatg gtaatcgtaa
     6481 tgataaccga caaggtcagc aaaataaccg taataaaaat gatgggcgtt atgctgatta
     6541 taaacagaaa ccccaaacgc gtcctcagca acctgcaggc aatcgcattg actttaaagc
     6601 tcgagcagca gctttgaaag ctgagcaaaa tgctgaatac tcacgtcata gtgagcagcg
     6661 tttccgtgaa gagcaagaag ccaaacgtca agcagctaag gagcaagaac tagctaaagc
     6721 agcagcgtta aaagcgcaag aagaagctca aaaagctaag gaaaaacttg cttctaaacc
     6781 tgttgctaag gtaaaagaaa ttgtcaataa agtagccgct acgccatcac agacagcaga
     6841 tagtcgtcgt aaaaaacaaa ctcgttctga taagtctcgt caattttcaa atgaaaatga
     6901 agatggtcaa aagcaaacaa aaaataagaa aaactggaat aatcaaaacc aagtgagaaa
     6961 tcaaagaaat agtaactgga atcataataa gaaaaataaa aaaggtaaga ctaatggagc
     7021 tccaaaacca gtaacagagc gtaaattcca tgaattacca aaagaatttg aatatactga
     7081 gggcatgacc gttgcggaaa ttgcaaaacg tattaaacgc gaaccagcag aaattgttaa
     7141 aaaactcttt atgatgggag ttatggcaac gcagaatcaa tcattagatg gagacactat
     7201 tgagctattg atggttgact acgggattga agcacatgct aaggttgaag ttgatgaagc
     7261 tgatattgaa cgcttctttg cagatgagga ttaccttaat cctgataact taacggaacg
     7321 tccgcctgtt gttactatca tgggacacgt tgaccatggt aaaacaactc ttcttgatac
     7381 tctacgtaat tcacgtgtcg ctactggcga agctggtgga attactcaac atattggtgc
     7441 ttatcagatt gaagaagctg gcaagaaaat tactttcttg gatacaccag ggcatgcggc
     7501 ctttacttct atgcgtgcgc gtggtgcatc agtaactgat attactatcc ttatcgttgc
     7561 cgctgatgat ggtgtcatgc cgcagacagt tgaggcaatt aatcactcta aagctgcggg
     7621 tgtcccaatt attgttgcta tcaacaaaat tgataaacct ggtgcaaatc cagaacgcgt
     7681 tatcagtgaa ttagctgagc atggtgtcat ctctactgca tggggtggtg agtctgaatt
     7741 tgttgaaatt tcagctaagt ttggtaaaaa tattcaagaa ttacttgaga cagttctttt
     7801 agttgctgaa atggaagaat taaaagcaga tgcagatgtt cgtgctattg gtactgttat
     7861 tgaagcccgt cttgataaag gtaagggagc cgttgcaacg cttcttgtcc aacaaggtac
     7921 tttaaatgta caagatccaa tcgttgttgg taatacattt ggacgtgttc gtgcgatgac
     7981 taatgatctt gggcgtcgtg tcaaagttgc gggtccatct acaccagttt ctattactgg
     8041 tttgaacgaa gctccaatgg ctggtgatca ttttgctgtt tatgcagatg agaaagctgc
     8101 gcgtgctgca ggtgaagaac gtgcaaaacg tgctctctta aaacaacgcc aaaacacgca
     8161 acgtgttagc ctcgagaact tatttgatac ccttaaagct ggtgaagtta agtcagttaa
     8221 tgttattatc aaagctgatg tgcaaggttc tgtagaagca cttgcagctt ctctccttaa
     8281 gattgatgtt gaaggagtaa aagttaatgt tgttcactca gcagttggtg ctattaatga
     8341 gtctgatgta actcttgctg aagcatcaaa tgctgttatt ataggattta acgtacgccc
     8401 tactccacaa gctcgtcaac aagccgatgc tgatgatgtc gaaattcgtc aacatagcat
     8461 tatctataaa gttatcgaag aagttgaaga agcgatgaaa ggtaaacttg atcctgaata
     8521 ccaagaaaaa atccttggtg aagccattat tcgtgagacc tttaaagttt caaaagtggg
     8581 aactattggt ggatttatgg ttatcaatgg taaagttact cgtgattcaa gtgttcgtgt
     8641 tatccgtgat ggtgttgtca tttttgatgg taaattagca agtcttaaac actataaaga
     8701 tgatgttaaa gaagtcggca atgctcaaga aggtggtcta atgattgaga attataatga
     8761 tctcaaagaa gatgacacta ttgaagcata cattatggaa gaaattaaac gtaaataacc
     8821 cctatgagaa acccaacttg tatctttagt tggtagggaa tacaaaatta tggctaggct
     8881 cgtcctagct ttcaagttat gagcacaatg aagaaaggat taattatggc taatcatcgt
     8941 attgatcgcg ttggaatgga aattaaacgt gaagtcaatg agattttgcg tctccgagtt
     9001 aatgatccac gtgtacaaga tgtcacaatt actgatgttc agatgttggg tgacctttca
     9061 atggctaaag tattttatac tattcattcg acacttgctt ctgataatca aaaagcacaa
     9121 attggtttag aaaaagcaac tggtactatt aagcgtgaac ttggcaaaaa ccttactatg
     9181 tataaaatac cagatttaca atttgttaaa gatgaatcta ttgaatacgg aaataagatt
     9241 gatgaaatgt tgcgaaatct agataaaaaa gattaagaat aataaaaaat aaaccatgtc
     9301 aaaatcatgg tttatttttt atagctttag agttgagata gctagtatta aggagtttta
     9361 ttgatgatat caatcatttg atactgtgct gttttagctt cttcaatagg gtaaattgga
     9421 taaacatgat tcattccctc ttgtgcaata tagttaacgg ggtgattagc tgcttggagt
     9481 tgtgcagcat aatcgcgaat atctgggaag aatatttctc gtgttccagt aaataacgta
     9541 ataggagcga gtttagtagc aggggcattt ttaggactaa catatgtatg gttagtatta
     9601 ttagaaccat ttgcccaaat ttcacctaca cgtgcgagac cccaagcaga taggatagga
     9661 tcggtatctt cgtatttggg aatttctgga tgtttcattg tcacatctag ccatggagat
     9721 aagaggataa tatttttagg ttgaggaata gcctcttgcc ctgattggtg agacaatgca
     9781 tgcgctaatc ctagtgctaa tcctccgcca gctgaatcac ccatcaaagt gagattagcg
     9841 tttttttcat gaaagtgtct ataaaggtta accaatctag ggatagcata atcataggta
     9901 taacgaggag tttttggata aattggtaaa ataatttttg catcaagact agttgagagc
     9961 gtcttaagca tgctgatatg gtaaggcgtt ggattattta agtaagagcc accagctaaa
    10021 tagaaaatgg tcttttgatt agctttaccg ttgtcattcc aagaatagac ttgcatatca
    10081 aaaagtctct cttgaacaag ccctccatta acagagtttg ggtctaagta ataaccctta
    10141 tcagattgtt cacggctagc atttaagtac ttttgaaatg agctttcatc ttgaacttta
    10201 taataacttt tatcgcgctt tagccaaaag tattgttcga taaaccaact tttccatgaa
    10261 cgcccacttg cttttgcatg tggtgtacag gcgatggata aaatggtgat aatactaaat
    10321 agtagcaata gttttttgaa ggacggtttc aaggtattct ccttaatata atgttatttt
    10381 tgtgtaatat tattataata aaaccaacat ctttgtcaat agaataaaga aagatgagaa
    10441 atgtttttcg gtttacaaat gtagatttat ttgctaaaat tggtttacaa acgtaaatcg
    10501 aaagaggtga gaaaatgact atttcaaacg cagaatggga aattatgaga gtggtttggg
    10561 cacagcagaa tacaaccagt aatgagatac tagcagtttt actagagaag tatgattgga
    10621 ctccttcaac tgtcaaaacc ttattaagaa ggctcttaga taaaggctac gtgagcagag
    10681 aaaaaatggg aaaaggattt agctattctt ctttaattga tgaagattta gctatgatgt
    10741 cagaggtaga tagtgtattg cagaaagttt gccagaccaa acatgttgct attgttagac
    10801 acttattgga aagtatacca atgacagaga aggatcgatt aaatttgcaa gcatcactag
    10861 aagctaagaa aggcaaaaca cttgaacgtg tagcttgtaa ttgcattcct ggtcagtgtc
    10921 aatgccatta gaaaggagaa aatatggcta atagaaaaga aacatttttg attgatggga
    10981 tgacttgtgc atcttgtgct ttaacgatag aaaaagctgt caacaagtta gaccatgtgg
    11041 atagtgcagt agtaaattta gcaacagaga aaatgacagt tacgtttgat gatacaacct
    11101 tatcgccaaa cgtgattgaa gagtgtgttt ctgagactgg atacgaagca tcgcttttca
    11161 aagaggaaac ttctaaaagc caatctgaac ggcaccaatt agcaattgaa aagatgtggc
    11221 atcgcttttg gatgtcagca gttgcaacta tacctcttct ctatatttcc atggagccaa
    11281 tgattaactt atggttacca tcatttttga tgccagataa gggacctcta aactatggtg
    11341 tgatacaatt attgttaaca ctaccggtga tgtattttgg gcgtatcttt taccaaaatg
    11401 gttttaaagc actttttaaa aggcatccca atatggattc attagttgct atagccacaa
    11461 cagcggcttt catttatagc ttatatggat tgtacgaaat attacagggt gacatacact
    11521 acgctcacca gctttatttc gaatctgttg cagttatttt aacacttatt actctaggaa
    11581 aatattttga aatattatcc aaagggcgta cttcggcctc aattgaaaaa ttattaactt
    11641 tatcagcaaa agaagcaaga gttatcaaag atggtgaaga ctatatggtt cctttggata
    11701 aagttaaaat aggtgaaaca attcttgtca aacctggaga aaaaattcct cttgatggcc
    11761 atgttgtagc aggagaatca agtattgatg aatccatgtt aacaggtgaa agtattccag
    11821 ttgaaaaaaa ggttggtagt aaggtttacg gtgccagcat taatgggcaa ggtagcttaa
    11881 cgattttcgt tgaaaaagaa gctgtcggtt cccttctatc acaaattatt aacttagtag
    11941 aagttgcaca ggcaagtaag gctccaattg ctaacttagc agacaaagta tcaggagtct
    12001 ttgtcccttt tgttattgtc attgcaattt tatcaggttt gagttggtac ttaattctgg
    12061 ggcaaagttt tgccttctct ctgaagatta tgattgcagt cttagtgatt gcttgtccat
    12121 gtgctttagg gttagcaact cctacagcta ttatggtggc tagtggtaaa gcagctgaga
    12181 atggtatttt atttaaaggt ggtgaagtgt tggaaaaggc tcaccacatt gatacaatag
    12241 tttttgataa aacaggaacc ttgaccaaag ggaaaccaga agtagttgct atcaaaacat
    12301 atggggtaga taaagaggag tttttaggtc aagtagcatc agtagaaaaa ctttcaaatc
    12361 atccacttag ccaaacaatt gttaacaaag caaaagagaa agaacttccc ttaagggaag
    12421 tcatggcttt taaaaatatt ttgggttacg gtttgagtgc aattattaat ggtaaaacta
    12481 tgcttgtagg taatgctaac ttaatgacta agaacgatgt taatttagat ttagcaaagg
    12541 ctgatataga aatagctcaa gaagaagcac aaactgtggt ttacgtttcc gaaaatggag
    12601 tgttgagcgg tttaataact ttaacggacc agctaaagac agatagccaa gaaacagtta
    12661 agcagttaca aagattagga tttaatttag ttttacttac tggtgataat aaggctagtg
    12721 cagatgccat agcacaaaaa ttgggtatca cgacagttgt ttcagaagtt ttacctgatc
    12781 agaaagcaaa cgttatttta gaacttaaag aaaaaggcgg acagattgcg atggttggtg
    12841 atggcatcaa tgatgctcct gcgctagcta gcagtgatgt aggtatctct atgagttctg
    12901 gaacagatat tgctattgaa tcagcagata tcgtgttgat gaaacctgag ttaacagatc
    12961 tcttaaaagc aatgactatc agtaaacaaa cgattcaaat tattaaggaa aatttatttt
    13021 gggctttctt ttataacgtt ttagcgattc cagtggcaat gggtgtttta catctttttg
    13081 gtggtccctt actcaatcct atgttggcag gcttagctat ggcgtttagt tcagtttcag
    13141 tagttttaaa cgcacttcgc ttaaaagtct taaaataata aagataataa gactgaattt
    13201 ttatgaaggg agaagaatat gaaacatacc tatcgagtta gtggcatgaa atgtgatggt
    13261 tgtgccaaaa cagtttctga taagttatca agtgtgatgg gtgttgacga ggttaatgtt
    13321 gatttgacta aaaatcaagt tgtcgtaagt ggaaaaacat tcaaatgtct tttaaaacgt
    13381 tctttaaaag acacaaagta ttcactagaa gaggaaataa attaataact aaagaagtgg
    13441 ttacagcttt taatttctta atttgagcag tttctcactt cttttttatt ctaaaaatga
    13501 taaactagaa tagaattagt tgtgaggagt gcagatgtca atcgatattt ggaacatttt
    13561 aagtttggtt ggaaccgtag cttttgcaag ttcgggagct attgtggcga ttgaggaaga
    13621 atttgatgtt ctagggcttt ttatcttagg ttttgtaacg gcatttggtg gtggagctat
    13681 tagaaatgta ctaattggct tgcctattga aacactttgg agtcaaggaa ttgcttttta
    13741 tgcagcagca gcagctattt tgtttattat gattttccct aacttgttga gtggtaaggg
    13801 acgggatgcc gaagttgtat cagatgctat tgggttagca gcgtttagtg ttcaaggagc
    13861 actatatgcg actcaatctc atcaacctct tagtgctgtc attgtcgcag cagtactaac
    13921 tggtgcaggt ggtggtattg tcagagatgt tctggctggc cgaaaacctg gggtactaag
    13981 aagtgaaata tatgcaggtt ggtcaatatt agtggggatc attttatatt ttaaaattgc
    14041 taaaacaaca actgactatt atttgttagt gttagttgta actagcctaa ggatgttggg
    14101 gtataagaaa caatggcact tgccagttgt cagaccacgt aagaaatgat agaggagaaa
    14161 gagatgataa aattaattgc tattgatatg gatggaacgc ttttaaatga tgaaaagaaa
    14221 attccaaagg aaaatatcca agctattaaa gaagcaactc aagcaggtat taaaattgta
    14281 ctgtgtactg ggcgaccgat gtcaggtata cttccttatt ttaatgaact aggtttaaca
    14341 aaagaggagt atattatcat gaataatggt tgtagcactt atagcacgaa agattggcaa
    14401 ttgattgatt ctgcaacttt aacacatgat gaactcatct ttcttgagga ggttgttaaa
    14461 gaatttccca acgtttgttt aacgttaaca gctgaaaata cattttacgc tgttggtgag
    14521 gaagttcctg aaattgttgc ttatgatgct gatttagttt ttacaaaagc aaagtcaata
    14581 agtttagatg ctcttcgcaa tcaagaagaa attgtctttc aagcaatgta catgggatta
    14641 gatgcagatg taactgcttt tcaagaagct gttgaagaag ccttaatctc gaaatttagt
    14701 ggagttcgaa gccaagacta tatttatgag attatgcctc aaggagtaac aaaagcaaga
    14761 ggcttaaaat ccttgatagc aaaattagga ctggatatta atcaagttat ggctattggt
    14821 gatgctccta acgatattga attattagat ttagttccca atagcgttgc tatggggaat
    14881 gcatcagatg agattaaatc tagatgtaaa tatataacgg tggataataa ccaagccggg
    14941 gttgctaaag ctatttatga ctatgcttta cgttaaaata gatatagaat aataaaagga
    15001 actttaaaca ctgtttgttt agggttcttt tgtttaataa actaacaaaa ttcagaagtt
    15061 tacagatagc ttatgctata atagacctat gacaaacaag aataaattac ttttaattga
    15121 tggctcatct gtagcctttc gtgctttttt tgctctttat aatcaaattg accgctttaa
    15181 aaacaattct ggacttcata ctaatgccat atatggtttc catttgatgt tgaatcatat
    15241 tttaggtcgt gtccagccta gtcatatttt agtggctttt gatgctggaa aaactacttt
    15301 ccgtacggaa atgtatgcag attataaagg tgggcgtgct aaaactccgg atgaatttcg
    15361 tgaacaattt ccttatattc gtcaacaatt agatgtctta ggcatcaaac attatgaatt
    15421 ggaacattat gaagccgatg atatcattgg tactcttgca aaacaagccg aagctagtaa
    15481 agagcatttt gatataacgg tggtttctgg ggataaagat ttaattcaat taacagacac
    15541 taacacggta gtagaaatct ctaaaaaggg agttgctgag tttgaagaat tcacgccagc
    15601 ttaccttatg gaaaaaatgg gaattacgcc gagtcaattt attgatttaa aagctttgat
    15661 gggagataaa tcagataata ttcctggggt tactaaaatc ggtgaaaaaa ctggcttgaa
    15721 gttgctatca gaatatggtt ccttagaagg gatctatgaa aatattgagg ctatgaaaca
    15781 gtctaagatg aaagaaaacc ttatcaatga taaggagcaa gctttcttat caaagaccct
    15841 agctacaatc aacatagcat cacctataac aatcggctta gaagatatcc tttatagtgg
    15901 accgcaagat ataaaagcct tgtctcaatt ttatgatgag atggatttta aacagtttaa
    15961 agcagctctg ggtgaggaga caagtcagga agattttgag gttgacttta ctgaagtgga
    16021 gcaattaaaa ccagaaatgt tctccgataa tgatttttat tactttgaga tgttaggtga
    16081 taactaccac gtagaagatt taattgggat agcttgggga aatagcgata caatctacgc
    16141 tactagtgat gtatcgttat tgcaagaagc tttatttaaa aaagctttat caaaacctat
    16201 caaaacttat gattttaaaa gaagtaaggt ccttttaaat cgttttaata ttgatttacc
    16261 agagccagcc tttgatacta gattggccaa gtaccttttg tctactactg aggataatct
    16321 agtatcaaca attgctaggt tatatacaaa tctgccactg gatactgatg atgctgttta
    16381 tgggaaaggt gctaaacgcg ctataccaga aaaaacacga tttcttgagc atctagctaa
    16441 aaaagtaaaa gttttagtgg attctgaggc taatattatg caacagctta aagctaatga
    16501 gcaagaagaa ttgctgtttg aaatggagca acctttagcg aatgttttag ctaaaatgga
    16561 aattcgaggt attaaggtta agaaaaatac cttgaatgaa atggctattg aaaatcaaaa
    16621 agtcatcgaa accttaacac aagagatcta tgaattggct gggcaagagt ttaatattaa
    16681 ttctccaaaa cagttaggaa agttgttgtt tgaaacgtta ggtcttcctg tagaaatgac
    16741 taaaaaaaca aaaacaggtt attcaactgc agtagatgta ttagagcgct tagctccgat
    16801 ttctcctctt gtaactaaaa ttttagagta ccgtcaaatc actaaattgc aatcaaccta
    16861 tattattggt ttacaagatt atattttaga agatggtaaa attcatacac gttatgttca
    16921 agatttgact cagacaggtc gtctttcaag ttctgaccca aaccttcaaa atataccagt
    16981 tagactagag caaggacggc ttatccgaaa ggcttttgtt ccatcagaag acaatgctgt
    17041 tttattgagt tcagattatt ctcaaataga attgcgcgtg ttagcacata tttcaaaaga
    17101 cgaacatttg atcgctgcct ttaaagaggg agcagatatt catacttcaa cagctatgcg
    17161 tgtttttggt atcgaaaaac ctgagaatgt cactcccaat gatcgtcgaa atgctaaggc
    17221 tgttaacttt ggaattgttt atggtatttc agattttggt ctatcgcata atttaggaat
    17281 acctcgtaaa ctagcgaagc aatatattga cacctatttt gagcgttatc ctggaataaa
    17341 aaactatatg gaaacagttg ttcgtgaagc aaaagataag ggttacgttg aaacactttt
    17401 ccatcgtcgt cgttcactac cagatattaa ttctcgtaat tttaacattc gccaatttgc
    17461 agagcgcaca gcaatcaatt caccaatcca aggaagtgca gctgatattc ttaaaattgc
    17521 aatgataaat ttagataggg tacttgataa aggtggttat aaatcaaaaa tgctgttgca
    17581 agttcatgat gaaattgtac ttgaagtccc taacgaggag ataggagcaa tcaaggagtt
    17641 ggttacaaaa acaatggagt cagcaattag tttatcggta cctttgattg cagatgagaa
    17701 cgcaggtgag acttggtatg aagcgaagta gtggacaaca tacgtaaagg agtcattaat
    17761 atggtttatc attttcaaaa tctaagtgat tttatgttaa aaaattattt aacaaaagca
    17821 aagaccatcg cagttgtagg attgtcagac cgtcaagaaa ctgcagctta tcaagtatca
    17881 aaaataatgc aagaagcagg ttatcaaatt attccagtta atcctaaaaa tgcaggacag
    17941 aaaatacttg ggcaaatgac atatgcctca cttaaggatg tcactgaaca tattgatatt
    18001 gttaatattt ttcgacgtag cgagtattta cctgatattg caagagaatt tttagaagta
    18061 gacgctgata ttttctgggc tcagcttgga cttgaaagtc aagaagcaga gacaattcta
    18121 aagcaagcag gacacaaaca gattgtcatg aataaatgtc tgaaagttga ataccaaaaa
    18181 ttacactcag aaactaacta attaccgtgt ttaataggca aataatataa attatgctat
    18241 aataaccatg ctaaattaaa gttcgtgctg gaaaggagtc ctatggacag tcattcacat
    18301 ggtcatagac ctctcgatgc ttatgaaaac gtcctagagc atctccgtga aaaacgtatt
    18361 cgaattactg agactcgaaa agctattatt tcctatatgg ttaatagtcg tgagcatccg
    18421 agtgcagaaa agatttacaa tgatttatta ccagaatatc ctaacatgag cttggcaaca
    18481 gtttacaata accttaaggt ccttgtggat gaggggtttg tgacagagct aaaactttgc
    18541 aattatagta caacttatta tgattttatg gggcatcagc acctaaacat tgcctgtgaa
    18601 gattgtggga aaatcgttga ttttgtcgat gttgacttat tggatatttc tagagaagca
    18661 caccaacaaa caggttttga agtgactaga gttcagttag tagcttatgg tatctgtccg
    18721 gagtgtcaga ggaagcgtaa ggataaatat aaaaaagatt gaaacgtaat tgttcaatct
    18781 ttttttgttt ctagactaag agaaaaagtt taagttttac taagtaataa ttaagaaata
    18841 atttataatt gcttaaaaaa tgatttattt tttgttggta tactctcatt aacaaaaaga
    18901 aagaggaatc ccttatgaaa atatcacaat acaataaatg gtcaattcgc cgattgaaag
    18961 ttggagctgc atctgtaatg attgctagtg gaagcattgt tgctttaggg caatctcata
    19021 ttgtttcagc agatgagatg tcccaaccta aaacaaccat tacagcccca acagctaaca
    19081 cttctacaaa tgtagaaagt agtactgata aggcattatc aaaagttact acgatggaga
    19141 caagttcaga aatgcctaaa atgcaaaata tggcaaaagt tgaaaaaacg agtgacaaac
    19201 ctatgatggt ggctacctct gttagaaaaa tgatggcaac gccgacacct gtagcgatga
    19261 ccaaaacgac tagtgtagat gaggtaaaga aatcaactga tactgctttt aaacagacag
    19321 tagacgttcc ggctcattat gttaatgcag caaaagggaa tggcccattt ctagcaggtg
    19381 taaatcaaac gattccatat gaagcgtttg gtggaga
//
LOCUS       DATROD010000025        19308 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.025, whole genome shotgun sequence.
ACCESSION   DATROD010000025 DATROD010000000
VERSION     DATROD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19308
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.025"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..1025
                     /locus_tag="VBC74_000884"
     CDS             <1..1025
                     /locus_tag="VBC74_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO6941847.1"
                     /translation="LILPLVISGLLYYLLKPMVTFLEKRGIKRVTAILSVFTIIILLL
                     IWAMSSFIPMMSNQLRHFMEDLPSYVNKVQMETSSFIDHNPWLKSYKGEISSMLSNIS
                     SQAVSYAEKFSKNVLDWAGNLASTVARVTVATIMAPFILFYLLRDSRNMKNGFLMVLP
                     TKLRQPADRILREMNSQMSGYVQGQIIVAITVGVIFSIMYSIIGLRYGVTLGIIAGVL
                     NMVPYLGSFVAQIPVFILALVAGPVMVVKVAIVFVIEQTLEGRFVSPLVLGNKLSIHP
                     ITIMFILLTSGAMFGVWGVFLSIPIYASIKVVVKELFDWYKAVSGLYTIDVVTEERSE
                     EVKNVE"
     gene            1015..2241
                     /locus_tag="VBC74_000885"
     CDS             1015..2241
                     /locus_tag="VBC74_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941848.1"
                     /translation="MLNSEKMIVSIQNQDLEHANKYFEKALKNDPEEILLELGAYLES
                     IGFLPQAKRLYDQIRPNYPEVAINLAQIVAEDGDIEQAFLYLDYISEDSQEYVSALLV
                     MADLYDMEGLTDVAREKLLLASKLSDDPLVTFGLAEMNLSLEHYQEAIEGYASLDNRE
                     ILETTGVSTYQRIGKSYAIIGKFDAAIEFLEKAVDIEYDDLTVFELATILYDQEEYQK
                     ANLYFKQLDTINPDFDGYEYIYGLSLREEHKSEEALRLVQQGLRKNSFDGQLLLLASQ
                     LSYELHDVPSSESYLKQAEKVSENQDEIVMRLSNLYLEEERFEEVLELDNDDLENILA
                     KWNIAKAHKALEMDDSVDYYQSLYNDLKDNPEFLQDYAYILREFGYLDKAQEVGKAYL
                     KLVPDDIEMSEWVNNI"
     gene            2351..4033
                     /gene="alsS"
                     /locus_tag="VBC74_000886"
     CDS             2351..4033
                     /gene="alsS"
                     /locus_tag="VBC74_000886"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEO6941849.1"
                     /translation="MTESHNQYGADLIVDSLINHDVKYVFGIPGAKIDRVFDTLEDKG
                     PELIVARHEQNATFMAQAVGRITGEPGVVIATSGPGISNLATGLVTATDEGDAVLAIG
                     GQVKRGDLLKRAHQSMNNVAMLEPITKYSAEVHDPNTLSETVANAYRLAKSGKPGASF
                     ISIPQDVTDSPVSVKAIKPLSAPKLGSASVLDINYLAQAINNAVLPVLLLGNGASSEG
                     VTAAVRRLLDAVKLPVVETFQGAGIVSRELEDETFFGRVGLFRNQPGDMLLKRADLVI
                     AIGYDPIEYEARNWNAEISARIIVIDVEQAEIDTYFQPERELIGDMAHTLDLLLPAIK
                     GYELPEGSKEYLKGLRNNIENVSDVKFDRDSAHGLVHPLDLIDVLQDNTTDDMTVTVD
                     VGSHYIWMARYFKSYEARHLLFSNGMQTLGVALPWAISAALLRPNTKVISVSGDGGFL
                     FSAQELETAVRLHLPIVHIIWNDGKYNMVEFQEEMKYGRSSGVDFGPVDFVKYAESFG
                     AKGYRVDSKDSFEETLKQALIDAENGPVLIDVPIDYKDNVTLGETILPDEFY"
     gene            4047..4766
                     /gene="budA"
                     /locus_tag="VBC74_000887"
     CDS             4047..4766
                     /gene="budA"
                     /locus_tag="VBC74_000887"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEO6941850.1"
                     /translation="MSETVKLFQYSTLSSLMAGLYKGSLTIGELLTHGDLGIGTVHMI
                     DGELIVLDGKAYQAIGTDGKAEIIQLSDDVTVPYAAVLPHHIQKQFDINAETDNKDLE
                     EMILKNFEGQNLFKSLKIKGTFSRMHVRMIPKSPQHKRFADIASNQPEFTRENVSGTL
                     VGIWTPELFHGVGVKGFHVHFISDDLTFGGHVMDYSLTQGKVEIGKVDQLDQCFPTQD
                     QEFLKANFDLQKLREDIDLSE"
     gene            complement(4820..6475)
                     /locus_tag="VBC74_000888"
     CDS             complement(4820..6475)
                     /locus_tag="VBC74_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEO6941851.1"
                     /translation="MSFDGFFLHHLTNELQEQIEKGRIQKVNQPFDHELVLTIRNNRR
                     NYKLLLSAHPVFGRIQTTEANFQNPQNPNTFTMIMRKYLQGAVIETIQQIENDRILEI
                     IVSNKNEIGDHIKATLVVEIMGKHSNIILIDKNEHKIIESIKHVGFSQNSYRTILPGS
                     TYIAPPKTKAINPFDISDQTLFELLQTNDLSPKNLQQLFQGLGRDTALELSHCLKDNK
                     LNDFRQFFSREYYPSLTEKSFSAVQFSSSHETFQSLGQLLDYYYQEKAEKDRIAQQAS
                     DLIHRVQSELEKNIKKLAKQQDELLATENAEEFRQKGELLTTYLSMVPNNQDIVVLDN
                     YYTNQTIEISLDRALTPNQNAQRYFKKYQKLKEAVKHLKGIISDTKNTITYLESVETS
                     LNHASMEDINDIREELVETGFIKRRAHDKQHKRKKPEQYLASDGKTIIMVGRNNLQND
                     ELTFKMARKGELWFHAKDIPGSHVLIRDNLNPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLNKPSGTKPGFVTYTGQKTLRVTPTQEKIDSLKLKK"
     gene            6834..7838
                     /locus_tag="VBC74_000889"
     CDS             6834..7838
                     /locus_tag="VBC74_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6941852.1"
                     /translation="MKNKGLIATLILLTILVVGELFYNKSEKHLNLSEKQVVKIGILQ
                     YVTHDALDAIEKGVEDGLAQEGYKGKKVKLTVLNAEADQSKIQAMSKQLVNHHNDILI
                     GIATPSAQGLAASTKDTPIIMGAVSDPLGAKLVTNMKKPTTNVTGLSNVVPTKQTVQL
                     IKDITPNVKRIGILYASSEDNSVSQVTEFTKYAQKAGLEVLKYSVPSTNEIKTSMSVM
                     TKKVDAVFVPQDNTIASAFRTVIVAANQANIPVYSSVDTMVEQGSIASVAQSQYGLGL
                     ETAKQAIKVLRGKPVKDVPVKVIDTGKPSLNLKAAKHLGIKIPKKIMKQAEITVKVDD
                     "
     gene            7851..8714
                     /locus_tag="VBC74_000890"
     CDS             7851..8714
                     /locus_tag="VBC74_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6941853.1"
                     /translation="MIISSVSQGLLWGILGLGIYLTFRILKFPDMTTEGSFPLGGAVC
                     VTLMNQGVNPILATILGMLSGMLAGFVTGLLYTKGKIPTILAGILVMTSCHSIMLMVM
                     KRANLGLNEIQTLKDFLPFSNDLNLLVLGLIAILLAISVLIYFLYTRLGQAYIATGDN
                     PDMAKSFGIDTDKMEMLGLIVSNGLIALSGALVSQQDGYADVSKGIGVIVIGLASIII
                     GEVLYSTGLTLFERLIAIVVGSILYQFLITAVIALGFNTNYLKLFSAIVLGICLMVPV
                     LKTKILKGVRL"
     gene            8714..9475
                     /locus_tag="VBC74_000891"
     CDS             8714..9475
                     /locus_tag="VBC74_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6941854.1"
                     /translation="MMMKIIELKEATVQVSNGLAEMKTILDHVNLSIYEHDFITILGG
                     NGAGKSTLFNVIAGTLMLSSGNIYIMGQDVTNLSAEKRAKYLSRVFQDPKMGTAPRMT
                     VAENLLVAKFRGEKRPLVPRKITNYTEEFQKLIARTGNGLDRHLETPTGLLSGGQRQA
                     LSLLMATLKKPNLLLLDEHTAALDPRTSVSLMGLTDEFIKQDSLTALMITHHMEDALK
                     YGNRVLVMKDGKIVRDLNQAQKNKMAIADYYQLFD"
     gene            9772..11433
                     /locus_tag="VBC74_000892"
     CDS             9772..11433
                     /locus_tag="VBC74_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEO6941855.1"
                     /translation="MSDIKIIALGGVRENGKNLYVVEVNDSIFVLDAGLKYPENEQLG
                     VDVVIPNLDYLIENKKRVQGIFLTHGHADAIGALPYIIAEVKAPVFGSPLTIELAKLF
                     VKNSTAVKKFNNFHVIDSETEIEFQDAVISFFKTTHSIPESMGIVIGTKEGNIVYTGD
                     FKFDQAARKYYQTDLARLAEIGRDGVLALLSDSANATSNEQVASEYEVGDEIKSVIED
                     AEGRVIVAAVASNLIRIQQVFDAAAENGRRVVLTGFDIENIVRTAIRMKRIHIADENM
                     IIKPKDMTRYEDNELLILETGRMGEPINGLQKMAIGRHRYVQIKDGDLVFIVTTPSIA
                     KEAVVARVENLIYKAGGSVKLITQNLRVSGHANGRELQLLMNLLKPKYLFPIQGEYRD
                     LSAHAGLAQEVGMSADDIYIVKRGDIMVLEKDGFFHSGSVPAGDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVVITVSKKEKKIISKARVNTRGFVYVKKSRDILRESAELVNTT
                     VEDYLSKDTFDWGELKGKVRDEVSKFLFDQTKRRPAILPVVMEVR"
     gene            11490..12278
                     /locus_tag="VBC74_000893"
     CDS             11490..12278
                     /locus_tag="VBC74_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEO6941856.1"
                     /translation="MAFFNIEYHSKVLGTERQVNVIYPDAFEMSDDKIDDCDIPVLYL
                     LHGMGGNENSWQKRTNIERLLRHTNLIVVMPSTDLAWYTNTKYGLDYFDAIAIELPKV
                     LKRFFPNMSDKREKNFIAGLSMGGYGAYKIALLTNRFSHAASLSGALSFDFDLLFNNG
                     NDNTNYWSGIFGDLNNTDNIERHSLRRYVESFDMKTKFYAWCGYEDFLFEANEVAIDE
                     LRQLGLTIDYSNDHGKHEWYYWNQQLEKVLEWLPVDYVKEERLS"
     gene            12368..13078
                     /locus_tag="VBC74_000894"
     CDS             12368..13078
                     /locus_tag="VBC74_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEO6941857.1"
                     /translation="MRTLFRMIFAIPKFIFRLIWNIIWGIFKTVLVIAIILFGLYYYA
                     NHSQSEFANQLSDIIQTGKTFLNFADTNQLKNSFANLATDNVHHSENTRWDKNQATIY
                     IATRDSELASAYKQAISNWNATGAFVLQTTDNPNADIIAKDYSDAKTQAAGVAETERN
                     ALTNRISKVTVKLNTFYLKNKQFGYDHTRIVNTAEHELGHALGLGHNDQQHSVMQSKG
                     SHYGIQEVDIQTLKNLYA"
     gene            13316..13987
                     /gene="rpiA"
                     /locus_tag="VBC74_000895"
     CDS             13316..13987
                     /gene="rpiA"
                     /locus_tag="VBC74_000895"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEO6941858.1"
                     /translation="MDELKKLAGVTAAKYVKNGMIVGLGTGSTAYFFVEEIGRRVKEE
                     GLQVIGVTTSNRTTEQARGLGIPLKSADDIDVIDVTVDGADEVDPDFNGIKGGGGALL
                     MEKIVATPTKEYIWVVDESKLVETLGAFKLPVEVVRYGSERLFRVFKSKGYCPSFRET
                     EGDRFITDMGNYIIDLDLKKIEDPKQLANELDHTVGVVEHGLFNGMVNKVIVASKNGL
                     DILEK"
     gene            14044..15255
                     /locus_tag="VBC74_000896"
     CDS             14044..15255
                     /locus_tag="VBC74_000896"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEO6941859.1"
                     /translation="MSQFDRIHLVVLDSVGIGAAPDANDFVNAGVPDGASDTLGHISK
                     TVGLAVPNMAKIGLGNIPRPQALKTVPAEENPSGYATKLQEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEDIITKIEDFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLEELYRICEYARSITMERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFEDTVLNKLDQAGIDTYAVGKINDIFNGSGINHDMGHNKSNSHGIDTL
                     IKTMGLSEFEKGFSFTNLVDFDALYGHRRDPHGYRDCLHEFDERLPEIVSAMRDKDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPSFTGNGLIPVGHFADISATVADNFGVDTA
                     MIGESFLQDLV"
     gene            15306..15713
                     /gene="arsC"
                     /locus_tag="VBC74_000897"
     CDS             15306..15713
                     /gene="arsC"
                     /locus_tag="VBC74_000897"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416958.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEO6941860.1"
                     /translation="MEKVRIYHNPNCGTSRNVLAIIRHCGIEPEIIYYLKTPPSKMAL
                     VELLLEMKLSVRELLRTDVPAYEKFNLESSSVTDEEMIDAMIQDPVLINRPIVVTSKG
                     AKLCRPCEEILTILPVKMEKDFVKEDGQIIQSL"
     gene            15752..16561
                     /locus_tag="VBC74_000898"
     CDS             15752..16561
                     /locus_tag="VBC74_000898"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO6941861.1"
                     /translation="MTLLEKINETRDFLQAKGVTAPEFGLILGSGLGELAEEIENPIV
                     VDYADIPNWGQSTVVGHAGKLVYGDLSGRKVLALQGRFHFYEGNTMEVVTFPVRIMRA
                     LACHSVLVTNAAGGIGYGPGTLMLIKDHINMIGTNPLIGENLEEFGPRFPDMSDAYTA
                     TYRQKAHQIAEKQNIKLEEGVYLGVSGPTYETPAEIRAFQTMGAQAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTQRIKEDFKGLVKSLVAEL"
     gene            16563..17819
                     /locus_tag="VBC74_000899"
     CDS             16563..17819
                     /locus_tag="VBC74_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEO6941862.1"
                     /translation="MVSRLYYAVKFMIAVLFMTVMAGVGAILMHYVLMFTEWLAFGDS
                     RENTLSLLNSVTPIKRVLSLTLVSFLASLSWYYLQIKPKQITSIKQQVVFKDFSVKKS
                     PYWLHIGHAFLQLIYVGAGGPIGKEGAPREFGAINAGKISDLLALKVLDKRLLIVSGA
                     AAGLSAVYQVPLASVFFAFETLALGISLKNIVTLLVSTFGAASIAQLVISTAPLYHIS
                     KMSLNSQSLAFMFLIVLCVTPIAISFRYLNQKVTERRIKNIKILLSLPVVSLIVSVLS
                     IIYPQILGNGNALVQEVFKGTTVSLIAILVVLKMIATLSTLYAGAYGGILTPSFSIGA
                     CLGFLLASISIPLLPHLSIVTSMLVGAAIFLAITMRAPLTAVGLVISFTGQSVITIVP
                     LTIAVLFATAYDYFIRKMRSLYVNPY"
     gene            17803..18513
                     /gene="deoD"
                     /locus_tag="VBC74_000900"
     CDS             17803..18513
                     /gene="deoD"
                     /locus_tag="VBC74_000900"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEO6941863.1"
                     /translation="MSIHIEAKQGEIADKILLPGDPLRAKFIAENFLEDAVCFNTVRN
                     MFGYTGTYKGHRVSIMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAINPDIHVRE
                     LVLAQAAATNSNIIRNDWPEFDFPQIADFKLLDKAYHIAKEMDITTHVGSVLSSDVFY
                     SNQPDRNMALGKLGVHAIEMEAAALYYLAAQHNVNALAMMTISDNLNNPEEDTSAEER
                     QTTFTDMMKVGLETLISE"
     gene            18522..19289
                     /locus_tag="VBC74_000901"
     CDS             18522..19289
                     /locus_tag="VBC74_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEO6941864.1"
                     /translation="MTDFSTALKVLVEQYSYHNAFLLLRKHGPLNSDLLFLLEMMKER
                     RELNIDFLFAHQEQVVILQEKYNIKLLHNSYDLELLANYIMDLEAKVKNGLIIDFVRS
                     VSPILYRLFMILLAQEVPHLHDYIYNARDDHYDTWKFKELKESNHPVLLAFSERWHDS
                     RLTSKSLAECLQLTDLDEEVKSTIIQLRQFEKSVRNPLAHLIKPFDEQELYRTTQFSS
                     QAFLDQIIFLAKVIGVEYDTVNFHYDTVNKLIIKILE"
BASE COUNT         6131 a         3049 c         3946 g         6182 t
ORIGIN      
        1 tgctgatatt gccgcttgta atttctggct tgctttatta cctattaaaa cctatggtta
       61 catttttaga gaagcgggga attaagcgtg taacagcgat attatcagtt tttactatta
      121 taatccttct gttaatttgg gcaatgtcta gttttattcc catgatgagt aatcaattac
      181 gccattttat ggaagatctc ccttcatatg tgaataaagt gcaaatggaa acaagttcgt
      241 ttatagatca caacccttgg ttaaaatctt ataaagggga aatatcgagc atgttatcta
      301 atatcagtag ccaagcggtc tcttatgctg aaaaattttc aaagaatgtt ttagattggg
      361 caggaaattt agctagtaca gttgcacgtg tgacagtagc aacaatcatg gctcccttta
      421 ttttgtttta tcttttaaga gatagtcgca acatgaagaa tggtttctta atggttttac
      481 caaccaaact acgccaacca gctgatcgta ttttgcgaga aatgaatagt caaatgtcag
      541 gatatgtgca aggacaaatc attgttgcta ttactgttgg tgttattttt tcaataatgt
      601 atagtattat aggccttaga tatggcgtga cattagggat tattgccggt gtgttaaata
      661 tggttcccta tttgggaagt tttgtcgccc aaattccagt gtttatctta gcgcttgtcg
      721 caggacctgt tatggttgtt aaagttgcga ttgtttttgt tattgagcaa actctagagg
      781 gacgctttgt ctcacctttg gttttaggta ataaacttag cattcatcca attacaatta
      841 tgtttatttt attaacctct ggagcgatgt ttggtgtttg gggagtattc ctcagtattc
      901 cgatttatgc atctatcaaa gttgttgtta aagaattgtt tgattggtac aaagctgtca
      961 gtgggctata tacaatagat gttgttactg aagaaagaag tgaagaagtt aaaaatgttg
     1021 aatagtgaaa aaatgattgt ttctatccaa aatcaggatt tagaacatgc taacaagtat
     1081 ttcgaaaaag cgttgaaaaa tgatcctgag gaaatcttat tggaactagg agcttatttg
     1141 gagagtattg gttttttacc ccaagcaaaa cgtctctatg atcaaatccg accaaattat
     1201 ccagaagttg ctatcaattt agctcaaatt gttgccgaag atggagatat tgaacaagca
     1261 ttcttatact tagattatat ctcagaagat agtcaagagt atgtttcagc cctcctcgtt
     1321 atggctgact tatatgatat ggaaggcttg actgatgttg cgcgtgaaaa gcttttgcta
     1381 gccagcaaac ttagcgatga cccattggtt acatttggtt tagctgaaat gaaccttagt
     1441 ttggaacatt atcaagaagc gatagaagga tatgcatcac ttgacaatcg agaaatctta
     1501 gaaacgactg gagtttcaac ttatcagcgt attggtaaat cctacgctat tatagggaaa
     1561 tttgatgcag ctatcgaatt tttagaaaaa gccgttgaca ttgagtatga tgatttaaca
     1621 gtttttgagt tagcaacaat cctttatgat caagaagaat atcaaaaagc taacctttat
     1681 tttaaacagt tggatactat taatccagac tttgatggct atgaatatat ctatggttta
     1741 tctcttcgtg aggagcataa atctgaagaa gctttacgtc tagttcagca aggtcttcgt
     1801 aaaaatagtt ttgatggtca acttctctta ctagcttcac aattatcgta tgaattacat
     1861 gatgttcctt cgtcggagtc ttatttgaaa caagctgaaa aagtttctga aaaccaagat
     1921 gaaattgtta tgagattgtc aaatttgtat cttgaagaag agcggtttga ggaagtcttg
     1981 gaattagaca atgatgattt agaaaatatc cttgcaaaat ggaatatcgc taaagcacac
     2041 aaggctttag aaatggatga tagtgtagac tattatcaat ctttgtataa tgatttaaaa
     2101 gataatccag aatttttaca agattatgct tacattttga gagaatttgg ttaccttgat
     2161 aaggcacaag aagtaggaaa ggcttattta aaattagttc cagacgatat agagatgtca
     2221 gaatgggtga ataacattta aataggaaaa cacttacaga aaaaaataaa aaattcacaa
     2281 gtaaattttc acaatctgat gtaaaaactg tgatataatt gaaccattct tgtgaaaaga
     2341 gggagataaa atgacagaat cacataatca atatggggct gacttaattg tcgatagcct
     2401 cattaatcat gatgtcaaat atgtttttgg tatcccagga gctaagattg accgcgtatt
     2461 tgacactcta gaagataaag gtccagagtt aattgttgct cgtcatgagc agaacgcgac
     2521 ttttatggct caggctgtcg ggcgtattac gggagaacca ggagttgtta ttgcaactag
     2581 tggacctggg atctcgaact tggcaacagg gttagttacc gcgacagatg aaggtgatgc
     2641 tgttcttgca attggtggac aggttaagcg aggagattta ttgaaacgtg ctcatcaatc
     2701 aatgaataac gttgctatgc tagaacctat tacaaaatat tctgctgagg ttcatgaccc
     2761 taatactttg tcagaaactg ttgcaaacgc ctatcgtctt gctaaatctg gtaaaccagg
     2821 agcaagtttt atctctattc ctcaagacgt tacagacagt ccagtctcag taaaagcaat
     2881 caaacctctt agtgcaccta aattgggttc ggcatctgtt ttagatatta attatttagc
     2941 gcaagctatt aacaatgctg ttttaccggt actgttatta ggaaatggcg catcttcaga
     3001 gggagtaact gctgccgtac gtcgacttct tgatgctgtt aaactaccag tagtagaaac
     3061 tttccaaggt gcgggtattg tgtcacgtga gctcgaggat gaaacattct ttggtcgtgt
     3121 aggcctcttc cgtaatcaac ctggagatat gcttttgaag cgtgcagacc tagttattgc
     3181 tatcgggtat gaccctattg aatatgaggc gcgtaactgg aatgctgaaa tttctgctcg
     3241 tattatcgtt attgacgttg aacaagcaga aattgatacg tatttccaac ccgagcgaga
     3301 actgattggg gacatggcac atacacttga tttactttta ccagctatta aaggatatga
     3361 attgccagaa ggttcaaaag agtatttgaa aggtcttcgt aataacattg aaaacgttag
     3421 cgatgttaaa tttgatcgtg actcagctca tggacttgtt catcctcttg atctcattga
     3481 tgtactccaa gataatacca ccgatgacat gactgttaca gttgatgtgg gaagccatta
     3541 tatttggatg gctcgctact ttaaatcata tgaagcgcgt cacttgctct tctcaaacgg
     3601 aatgcaaaca ttaggagttg ctctaccttg ggcaatttct gcggctcttt tgcgtcccaa
     3661 cacaaaagtt atttcagttt ctggtgatgg tggcttctta ttctcagctc aagagttaga
     3721 aactgcagtt cgtcttcatc taccaattgt tcacattatt tggaacgatg gaaaatataa
     3781 catggttgaa ttccaggaag aaatgaagta tgggcgttct tcaggtgttg actttggacc
     3841 agttgatttt gtaaaatacg ctgaaagttt cggtgctaaa ggttaccgtg tagacagcaa
     3901 agacagtttt gaagaaacac tcaaacaagc tctaatcgat gctgaaaatg gaccagtgtt
     3961 gattgatgtg cctattgact ataaagataa cgtgacacta ggtgaaacaa tcttacctga
     4021 cgagttctac taagacgagg tctaatatgt cagaaacagt taaattattt caatacagca
     4081 ccttgagttc actgatggca ggattgtaca aaggcagtct aactatcgga gaattattaa
     4141 ctcacggtga cttaggaatc ggtactgttc atatgattga tggtgaattg attgtattgg
     4201 atgggaaagc ttatcaagct attggtacag atggtaaagc agagattatc caattatcag
     4261 atgatgtgac agtaccctat gcagcggttt taccacacca tatccaaaaa caatttgata
     4321 tcaatgctga gacagataat aaggatttgg aagagatgat tctaaaaaac tttgaaggtc
     4381 aaaatctctt taagtcttta aaaatcaaag gaacgttttc aagaatgcac gttcgtatga
     4441 ttccaaaatc tccacaacat aaacgttttg cagatattgc atctaatcag ccagaattta
     4501 ccagagagaa tgtatctggt acgttggttg ggatttggac gccggaatta tttcatggtg
     4561 tgggggtaaa agggttccat gtccacttta tttcagacga tttaactttc ggtgggcatg
     4621 ttatggatta ctcgctcaca caaggaaaag ttgaaattgg aaaggttgac caacttgacc
     4681 aatgtttccc aactcaagat caagaatttt tgaaagctaa ctttgaccta caaaagctta
     4741 gagaagatat tgatttaagt gaataagaat acctataaat aattaataac actaaaaata
     4801 gacagggata tgtcccagtc tattttttta gtttaaggct atctattttt tcttgtgtgg
     4861 gagtgactcg taaggttttt tgaccagtgt aagttacaaa acctggctta gtaccacttg
     4921 gtttattgag tttttttgct tcaatcatgt ctacttgaac aagattggag agacgagctt
     4981 tagagtaata agcagctaac tcggcggcat ctgtcttaac ctcgtcactt ggattgaggt
     5041 tatcccgaat taacacatgg cttccgggga tatccttggc atgaaaccac aactcccctt
     5101 tacgagccat tttaaatgtt aactcgtcat tttggagatt attacgtccc accataataa
     5161 tagtcttgcc atcggatgct aaatattgtt caggtttttt acgcttatgt tgtttatcat
     5221 gtgcgcgtcg cttaataaat ccagtttcaa caagttcttc acgaatatca ttgatatctt
     5281 ccatagaagc atgatttagc gatgtttcaa cagattcaag gtaggtgatt gtatttttcg
     5341 tatctgaaat aattcctttt aaatgtttta cagcttcttt taatttttga tattttttaa
     5401 agtagcgttg ggcattttgg ttgggtgtta aagctcgatc aagtgaaatt tcaatcgttt
     5461 ggttggtgta ataattatca agaacaacga tatcttgatt atttggcacc atagagagat
     5521 aggtagttag aagctctcct ttttggcgaa actcctctgc attttcagta gctagaagtt
     5581 cgtcttgttg cttagctagt tttttgatat ttttctctaa ctcactttga acacggtgaa
     5641 tgaggtcact agcttgctgt gctatgcgat ctttttcagc cttctcttgg tagtaataat
     5701 ctaacaattg tccaagagac tgaaatgttt cgtgactgct tgagaattgg acagcggaaa
     5761 acgatttttc tgttaggcta ggataatatt ctctcgaaaa gaattgacga aaatcgttaa
     5821 gtttattgtc tttcaaacag tgagataatt ctagcgcagt atcgcgtcct aaaccttgga
     5881 agagctgttg gaggttttta gggcttaggt cattggtttg aagcagctca aatagagttt
     5941 gatcagaaat atcaaaagga ttgattgctt ttgttttggg tggggcaatg taagttgaac
     6001 caggaagaat agtacggtag gagttttgcg agaaaccaac atgcttaatt gattcaataa
     6061 ttttatgttc atttttatca atcaagataa tgttactgtg ttttcccatg atttcaacaa
     6121 caagtgtcgc tttaatgtga tcaccgattt cgtttttatt agacacgatg atttctaata
     6181 tacggtcatt ttcaatttgt tgaatggttt caatcactgc accttgcaaa tactttctca
     6241 tgatcattgt aaaagtattt ggattttgag gattttggaa atttgcttcg gtagtttgga
     6301 tacgtccaaa tacagggtga gcagatagga gtagtttata gttgcgacga ttattgcgta
     6361 ttgttaatac caattcatgg tcaaaaggtt ggttaacttt ttggatacgt cctttttcaa
     6421 tctgttcttg taattcattt gtgaggtgat gtaaaaaaaa tccatcaaaa gacatggcgt
     6481 cccccttatt ctaaaaatta tagcttattt attataccat aggctgagct aaaaatattt
     6541 ttttataacg caaaaggctt gcaattttca gaatatagta tataattata gaaaaattca
     6601 tgagaaagga tgactagtaa atgactaaaa cattatttac caattggcaa aactggcgta
     6661 gataaagttg tgtttatggg tgcatagata cgactttttg gagtacttca aaaaagtatc
     6721 tatgcgctgg tctagtctaa caatagagag cgcaaggtaa gttatttatc aggcgctttt
     6781 tcttttgtgc taaactccag aaagtcagga tattattaaa gtgaggagaa aatatgaaaa
     6841 ataaaggact gatagctacg ctaattctat tgaccatatt agtggttggg gaattatttt
     6901 ataacaaatc agaaaaacat cttaatttat cagaaaagca agttgttaaa attggtattt
     6961 tacagtatgt aacccatgat gctttggatg ctattgaaaa gggagttgaa gatggtctag
     7021 ctcaagaagg atataaaggg aaaaaagtta aattaaccgt tttaaacgcc gaagctgatc
     7081 aaagtaaaat tcaagcgatg agtaaacaat tagttaatca tcacaatgat attttgattg
     7141 gaattgcgac accatcagca caaggtttag cagcttcgac taaggatact cctataatca
     7201 tgggagctgt ttctgatcct ttaggggcca agttagtaac gaatatgaag aaaccaacaa
     7261 ctaatgttac aggattatca aacgttgttc cgaccaagca aactgtgcaa ttaatcaagg
     7321 atataactcc caatgttaaa cgtataggaa ttctttacgc tagtagcgaa gataattctg
     7381 tgtcacaagt aactgagttt acaaaatatg ctcaaaaagc agggctagaa gttcttaaat
     7441 attctgtacc atctacaaat gaaattaaaa caagtatgtc agtcatgacc aaaaaagtag
     7501 acgctgtctt tgttccacaa gataatacta ttgcgagtgc ttttagaaca gttatcgtag
     7561 cagcaaacca agctaatata cctgtgtatt ccagtgttga taccatggtt gaacaaggaa
     7621 gtatagcatc agtagcgcag agtcaatatg gcttagggtt agaaactgca aaacaggcca
     7681 ttaaagtcct aagagggaag ccggttaaag atgtaccagt caaagttatt gatacaggga
     7741 agccaagcct caatttgaaa gcagccaaac acttaggaat taaaattcct aagaagatta
     7801 tgaaacaggc agaaattacc gttaaggttg acgattagaa agagggtttt atgattattt
     7861 catcagtttc acaaggttta ttatggggga tattaggatt agggatatac ttaaccttta
     7921 gaatcttaaa atttccagac atgacaacag agggctcctt ccctctaggg ggagcggttt
     7981 gtgttacctt aatgaatcaa ggggttaatc ctattttggc gacgattcta ggcatgctat
     8041 cagggatgtt agcgggattt gttactggtt tactttatac taaaggaaag ataccgacga
     8101 ttttagcagg gattttagta atgacctcat gccactctat tatgctaatg gttatgaaaa
     8161 gagcaaatct aggtttaaat gagattcaaa cattgaaaga ttttcttccg ttttcaaatg
     8221 atttaaatct tctcgtatta ggtttgatag ctattctctt ggcgatttct gtcttaattt
     8281 attttctcta tacacgcttg gggcaagctt atattgcaac aggagacaac cctgatatgg
     8341 caaagagctt cggcattgat accgacaaaa tggaaatgct tggcttaatt gtatcaaatg
     8401 gcttaattgc tttatcagga gctttagtaa gtcaacaaga tggatatgct gatgtatcaa
     8461 aaggtatagg tgttattgtt attgggttag ctagtattat tattggagag gtactttact
     8521 ctactggttt aactctcttt gaacgtttaa ttgctatagt ggtgggttct attctttatc
     8581 aatttttaat aacggcagta atcgccttag gatttaatac gaattaccta aaattattta
     8641 gtgcaatagt gcttggaatt tgtttaatgg ttcctgtcct aaaaacgaag attttgaaag
     8701 gagtacgctt ataatgatga tgaaaatcat agagttaaaa gaagcaaccg tacaagtcag
     8761 taatggttta gcagaaatga aaacgatatt agatcatgtt aatttgagta tttatgaaca
     8821 tgacttcatt acaatattgg gtggaaatgg agctggaaaa tcaacgcttt ttaatgtaat
     8881 tgcaggtacc ttgatgttaa gcagtggaaa tatttacatc atgggacaag atgttactaa
     8941 tttatcagca gaaaaacgag ctaaatattt atcacgggta tttcaagatc cgaaaatggg
     9001 aacagcacct aggatgacag ttgctgaaaa tttattagtt gctaaatttc gaggtgaaaa
     9061 gagaccgtta gttcctagaa agattactaa ctataccgaa gaatttcaaa aattgattgc
     9121 tagaactggt aatggacttg atcgccattt agagacacct acaggtttat tatcaggtgg
     9181 acaaagacaa gcgcttagtt tattaatggc aactttgaaa aaaccaaatc tgttattatt
     9241 ggacgaacac acagcagcgc ttgatcctag aacaagtgtt tcgcttatgg gcttgactga
     9301 tgagtttatc aaacaagatt cgttgactgc cttaatgatt acacatcata tggaagatgc
     9361 tctcaaatat ggaaatcgtg tattagtaat gaaagatggc aagattgtca gagatttaaa
     9421 tcaggcacaa aaaaacaaga tggctatagc tgactattat caattatttg attaaaaaga
     9481 agtgaaatgg aagtgtttca cttctttttc tatacacttt gcaagaaatt attttcaaaa
     9541 tatgatatac taataatgct gtaaatcgac ttgtagagac aaggaccgta agtacaaatg
     9601 gaatgaaaat aaggacacac tctttaattc atattcctat tattgattaa taaggttttt
     9661 cttcaaaccc aagaaaagtt accgataaat caattactaa tgataataat tggacaattt
     9721 cttgtctgct ttataaaaaa gaattaacaa aatttaagga gaaaactcat tatgagcgac
     9781 ataaaaatca ttgctcttgg aggtgttcgt gaaaacggaa aaaacctata tgtagtagaa
     9841 gtcaatgatt ctatctttgt tttggatgct ggactcaaat atcctgaaaa tgaacaactg
     9901 ggtgttgatg ttgttattcc aaatcttgat tacttaattg aaaataaaaa acgtgttcag
     9961 gggattttct taacccatgg acatgcggat gctattggtg cattgcctta tattatcgcc
    10021 gaagtcaaag cacctgtgtt tggttcacct ctcacaattg aattagctaa gttattcgtt
    10081 aaaaatagta cagcagtcaa gaaattcaat aatttccatg tgattgatag tgaaacggaa
    10141 attgaatttc aagatgcggt gatttcgttc tttaaaacga ctcattcaat tccagagtca
    10201 atgggaattg ttattgggac aaaagaggga aatatcgttt acacaggtga ctttaaattt
    10261 gatcaagctg caaggaaata ttaccaaaca gatctggcgc gtttagctga aattggtcgt
    10321 gatggtgttt tagcactttt atcagattct gcaaatgcta caagcaatga gcaagttgct
    10381 agcgaatatg aggtcggtga tgaaattaag agcgttattg aagacgcaga aggtagagtc
    10441 atcgtagcgg cagttgcaag taatctcatt cgtattcaac aagtctttga tgcagcggca
    10501 gaaaacggtc gtcgagtagt tttaacgggt tttgatattg aaaatattgt tcgtacggcc
    10561 attcgtatga aacgcattca tattgctgat gaaaatatga ttatcaaacc taaagatatg
    10621 acacgttacg aagataacga attattaatc cttgaaactg gacgtatggg agagccgata
    10681 aatggtctcc aaaaaatggc gatcggacgt catcgttatg ttcaaattaa agatggtgat
    10741 ttagtattta tcgtaacaac accaagtatt gcaaaagagg cagttgtagc gcgtgttgaa
    10801 aatctgattt ataaagctgg tggctcagtt aaattgataa cacagaattt gcgcgtatca
    10861 ggtcatgcaa atggtcgcga attacaatta ttaatgaatc tattgaaacc taaatacctt
    10921 ttcccaattc aaggagagta tagggatttg tcagcacatg cgggcttagc tcaagaggta
    10981 ggaatgtcag cagatgatat ttatattgtg aagcgtggtg acatcatggt tcttgaaaaa
    11041 gatggcttct tccatagtgg atctgttcct gctggagatg ttatgattga tggaaatgct
    11101 attggtgacg ttggtaatat tgtacttcgt gaccgcaagg tattatctga agatggaatt
    11161 tttattgttg ttataactgt cagcaaaaaa gaaaagaaaa ttatttctaa agctcgtgtt
    11221 aatacgcgtg gttttgtgta tgtgaaaaaa agcagagata ttttgcgtga gagcgcagaa
    11281 ttagttaata caacggtaga agattactta agtaaagata catttgactg gggtgaactt
    11341 aaagggaaag tgcgtgatga ggtcagcaag ttcttatttg accaaacgaa acgtcgccca
    11401 gcaatccttc ctgtggttat ggaagttcgt tgatagtgtt aagaggttac caaattttgg
    11461 taacctttct tgctagaatg gaggttttta tggcattttt taatattgaa tatcactcta
    11521 aggtactagg aacggaacgt caagtcaatg ttatttaccc agatgctttt gagatgtctg
    11581 atgataaaat tgatgattgt gacattcctg ttttgtatct tctccatgga atggggggaa
    11641 atgagaattc ttggcaaaaa cgcacaaata tagagcgtct tttacgtcat actaatctta
    11701 tcgtcgtgat gccatcaaca gacttagcat ggtataccaa cacaaagtat gggttggatt
    11761 attttgatgc aatagctatt gagttaccaa aagttttgaa acgtttcttt cctaatatgt
    11821 ctgataagag agagaaaaat ttcattgccg gattatctat ggggggatat ggagcatata
    11881 aaatagctct actgactaat cgtttttctc atgctgcctc tttgtcaggt gcactttctt
    11941 ttgactttga tttattattc aataatggca acgataacac taactattgg tcgggaattt
    12001 ttggtgattt gaataacact gataacatag agcgtcattc actaaggcgt tacgttgagt
    12061 cttttgatat gaagactaaa ttttatgcat ggtgtggtta tgaagatttt ctttttgaag
    12121 ctaatgaagt cgcaattgat gaacttcgtc aattaggttt aactattgac tattccaatg
    12181 atcatggtaa acatgaatgg tattactgga atcaacaatt agaaaaagtc ttagaatggc
    12241 tccctgttga ttatgtcaaa gaagagcgcc tttcttagta ttagctaaat cgttgaaatc
    12301 caagtaaagt cttgttatac taaatcatta agttttggga aattagacac agaaggtgag
    12361 ataaaaaatg agaacactat ttagaatgat atttgctatt ccaaagttta tctttagatt
    12421 gatttggaat atcatttggg gaatattcaa gacagttctt gttattgcga ttattttatt
    12481 tggcttgtat tactatgcga atcacagtca atcagaattt gctaatcaac ttagtgacat
    12541 tattcagaca ggaaaaacat ttttaaattt tgctgacact aatcagctca aaaattcctt
    12601 tgctaatcta gctacggata atgttcatca ttcagaaaat actagatggg ataaaaatca
    12661 ggcaactatc tatatagcaa ctagagattc tgagttggcc tctgcttaca agcaagcaat
    12721 ctccaattgg aatgcaacag gtgcttttgt gttacaaaca acagataatc ctaatgctga
    12781 tattattgct aaggattact cagatgctaa aacacaagct gcaggtgtag ctgaaaccga
    12841 gagaaatgct ttaactaatc gtatttcaaa ggtaacagtt aaattaaaca cattttacct
    12901 taagaataaa cagtttggtt atgaccatac taggattgtt aatacggccg aacatgaatt
    12961 aggacatgct ttaggtctag gacataatga tcaacaacat tccgttatgc agtcaaaagg
    13021 ttcgcattat ggcattcaag aagttgatat tcaaacgcta aaaaacttat atgcataaaa
    13081 agatgagcta ataaactcat cttttttgtt tgaacactta ttttaggtga tataagtgcg
    13141 ttagcaatag attattaaaa ataaagaatt tgactatcac tactaactaa aatgcataaa
    13201 gaacaaagaa aatgttttca aaaatcactt aaaaaatggt ataatgtgtc ttgagatagt
    13261 gttttgaata gacttatcaa aaatattaga taacattaac ttaaaagggg aaaatatgga
    13321 cgaactaaaa aaacttgctg gagtgaccgc tgcaaaatac gttaaaaacg gtatgattgt
    13381 gggtttaggg acaggttcta cagcatattt ttttgttgaa gaaattggac gccgtgtcaa
    13441 agaagaggga ttgcaagtca taggcgttac aacgtcaaac cgtacgactg agcaggcaag
    13501 aggattaggt attccgttga aatcagcaga tgatattgat gtcattgatg tcactgtgga
    13561 tggagcagat gaagtagacc ctgattttaa tggtatcaag gggggcggtg gtgcgctgct
    13621 catggaaaaa atagtagcta ctccaactaa agaatatatc tgggttgtcg atgagagcaa
    13681 acttgttgaa acgttaggtg catttaaatt gcctgttgag gttgttagat atggttctga
    13741 gcgtcttttt cgggttttta aatcaaaagg ttattgtcct tctttccgtg aaacagaagg
    13801 tgaccgcttt atcactgata tggggaacta catcatcgac ctagatttga aaaaaataga
    13861 ggatccaaag cagttagcga atgaacttga ccatacagtt ggtgttgttg aacatggttt
    13921 atttaatggt atggttaata aagttatagt agcaagcaaa aatggtcttg acatacttga
    13981 aaaataatta tttaaataat cggttttagg agctaaccgt aacctcctta gaaagaagat
    14041 ataatgtcac aatttgatcg cattcactta gttgtcttag attcagtggg tattggagcg
    14101 gcaccagatg ccaatgattt tgtcaatgca ggagttcctg atggtgcttc agacacgctt
    14161 ggccacattt caaaaacagt tggacttgcg gtgccaaaca tggctaaaat cggacttggt
    14221 aatattcctc gtccccaagc gcttaagacc gtccctgcag aagaaaatcc aagtggctac
    14281 gcgacgaagt tacaagaagt atcacttggt aaggatacaa tgacagggca ttgggagatc
    14341 atgggcttaa atattacaga gccatttgat actttctgga atggttttcc agaagatatt
    14401 atcacaaaga ttgaggattt ttcaggacgt aaagttattc gtgaagcgaa taaaccttat
    14461 tcagggacag ctgttattga tgacttcgga ccacgccaaa tggaaactgg tgagctcatc
    14521 atctatacct cagctgatcc agtcctacaa attgcagctc atgaggatat catccctcta
    14581 gaagaactct accgtatttg tgaatatgct cgtagtatca caatggagcg tccagctctc
    14641 cttggtcgta ttatcgctcg tccatatgtg ggtgaaccag gcaactttac gcgtacagcc
    14701 aaccgtcacg actatgcagt atcaccattt gaagataccg tattaaataa actagatcaa
    14761 gctggcatcg atacttatgc tgttggtaaa atcaatgata ttttcaatgg ttcaggcatt
    14821 aatcatgata tgggacataa caaatcaaat agtcatggta ttgatacttt gataaaaacg
    14881 atggggttat cagagtttga aaaaggcttc tcctttacta atttggttga ctttgatgct
    14941 ctctatggtc accgtcgtga tcctcatggt tatcgtgatt gtcttcatga gtttgatgag
    15001 cgtcttccag agatcgtttc agcgatgcgt gacaaagact tacttttgat cactgccgat
    15061 catggtaatg acccaactta tgcaggtaca gaccatacaa gagaatacat tcctctctta
    15121 gcctatagcc catctttcac aggaaacggc cttatcccag tagggcattt cgcagatatt
    15181 tcagcaacag ttgcggataa ctttggtgtt gatactgcga tgattggaga gagcttcttg
    15241 caggacttgg tataaatttt tatttcctta agacacttgt agcattaaaa attgagagga
    15301 agatgatgga aaaagttagg atttaccata acccaaattg tgggacatca aggaacgtac
    15361 ttgccattat tcgtcattgt ggtattgaac cagaaattat ttattatctg aaaacaccac
    15421 ctagcaagat ggcactagtt gaactgttgt tagaaatgaa actttctgtg agagaacttc
    15481 taagaacaga tgttcctgct tatgagaaat tcaatttgga atcttctagc gttacagatg
    15541 aagaaatgat tgatgcgatg atacaagatc ctgttcttat caatcgtccc atagttgtta
    15601 cgagtaaagg tgcaaaactc tgccgaccct gtgaagaaat tttaaccatt ttacctgtaa
    15661 agatggaaaa agattttgtt aaagaagatg ggcagattat ccaaagttta tagcatattg
    15721 tatagtgaat aatagtttag gaggtaagta aatgacatta ttagaaaaaa ttaatgagac
    15781 tagagacttt ttgcaagcaa aaggcgtcac agcaccagaa tttggtctta ttttaggctc
    15841 tggtttagga gaattagctg aagaaatcga aaatcccatt gttgtggatt atgcagacat
    15901 cccaaattgg ggacagtcaa cagtagttgg tcatgctgga aaattagtgt atggggattt
    15961 atcaggccgt aaggtattag cgcttcaagg tcgttttcac ttttatgaag gaaatacaat
    16021 ggaagtcgtt actttcccag tacgtatcat gagagcattg gcttgccaca gtgtgcttgt
    16081 gactaatgca gcgggtggga ttggatacgg accaggaact ttaatgctga tcaaagacca
    16141 catcaatatg attgggacta accctctcat aggtgagaac cttgaagaat ttggaccacg
    16201 tttcccagac atgtcggatg cttatacagc aacatatcga caaaaagctc accaaattgc
    16261 tgaaaaacag aatatcaaac tcgaagaagg tgtgtacttg ggtgtatcag gacccactta
    16321 tgaaacacct gcagaaattc gtgcattcca aacaatgggc gcacaagcgg taggtatgtc
    16381 cacggttcca gaggtgatcg ttgcagctca ctcagggctt aaagtgttag gaatttcagc
    16441 aattactaac tttgccgctg gcttccaatc agagctcaat catgaggagg tcgttgaagt
    16501 tactcagcgt attaaagaag atttcaaggg attagttaaa tcattagttg ctgaacttta
    16561 aaatggtttc tcgattatac tatgctgtca agtttatgat agccgttcta tttatgacag
    16621 ttatggcagg agttggagct atcttaatgc actatgtatt aatgtttaca gagtggttag
    16681 catttggtga tagtagagaa aatactctgt ctttgttaaa tagtgtcaca ccaattaaaa
    16741 gagtgttatc acttacttta gtctcctttt tagcatccct tagttggtat tacttacaaa
    16801 tcaagccaaa gcaaataact agtattaaac aacaggttgt ttttaaggat ttttctgtaa
    16861 aaaaatcacc ttactggcta catataggtc acgcattttt gcaattgatt tatgtaggag
    16921 ctggtggtcc tattggaaaa gaaggagcgc cacgagagtt tggagccata aatgctggga
    16981 aaatatcgga tttattagca cttaaagtgc tggataaacg tttattaatc gttagtggtg
    17041 cagctgcagg tttgtcagca gtttatcaag tacccttagc tagtgtattc tttgcttttg
    17101 aaactttagc tttaggaatt tcactgaaaa atattgtgac attgctagtc tctacttttg
    17161 gtgcagctag tattgctcaa ttagttattt cgacagcccc tctttatcat atttcaaaga
    17221 tgagtctgaa ttcacagtca ttagctttta tgttcttgat cgttttatgt gtaactccga
    17281 ttgcgattag ctttagatat ttaaatcaaa aagtgacaga gagacgtatt aaaaatataa
    17341 aaatattgtt aagtttgcca gtagtgtcat tgatagtatc tgttttatca ataatctatc
    17401 cccagatttt aggaaatggt aatgccctag ttcaagaagt ctttaaaggg accacagtta
    17461 gcttaatcgc tattttagtt gttctcaaga tgatagcaac attatccact ctttacgctg
    17521 gtgcttatgg cggtatactg acaccatctt tttccattgg tgcttgttta ggttttttgt
    17581 tagctagtat ttctattcct ttacttcccc acctatcgat agtaaccagt atgttggttg
    17641 gtgcagctat ttttttagct attacaatga gagcgccact gacagctgtc ggtttagtca
    17701 tatcttttac tggtcaatct gttataacta ttgttcccct aactatagca gtactttttg
    17761 ctactgccta tgattatttt ataagaaaaa tgaggagttt atatgtcaat ccatattgaa
    17821 gcaaaacaag gtgaaattgc agataaaatt ttacttccag gagatccact ccgtgcgaaa
    17881 tttattgccg agaatttttt agaagatgcg gtgtgtttta atacagttcg caatatgttt
    17941 ggttatacag gtacctataa aggacatcgt gtttctatta tgggaactgg tatgggtatg
    18001 ccttcaattt caatttatgc tcgcgaacta attgttgatt atggggttaa aacattgatt
    18061 cgcgtaggaa cagcaggtgc tattaatcct gatatccatg ttcgtgaact tgttttggca
    18121 caagcagcag caacaaattc taatattatc cgtaatgatt ggcctgaatt tgattttccg
    18181 caaattgctg attttaaact tttggataaa gcttatcaca tcgcaaagga aatggatatt
    18241 acaactcatg taggcagtgt attatcttca gacgtgtttt attcaaacca accagatcgt
    18301 aatatggctt tgggtaaact aggtgttcat gctattgaga tggaagcagc agctctctac
    18361 tatcttgcag ctcaacacaa tgttaatgct cttgctatga tgacgatttc agacaacttg
    18421 aataatccag aagaagatac aagtgcagaa gagcgtcaaa caacatttac tgatatgatg
    18481 aaagttgggc tcgaaacact tatttctgaa tgaggataga aatgacagat ttttcaactg
    18541 ctcttaaagt attagtggaa cagtattctt atcataatgc tttcttatta ttacgaaaac
    18601 atggtccttt aaactcagat ttattatttt tattagaaat gatgaaagag cgtcgagagt
    18661 taaacattga ttttcttttt gctcatcaag aacaagttgt tatattacaa gaaaaataca
    18721 atataaagct tttgcataac tcgtatgatt tagagttgct ggcaaattac atcatggatt
    18781 tagaagcaaa agtaaaaaat ggattaatca tagattttgt ccgtagtgtt agtcctattt
    18841 tatatcgtct atttatgatt ttacttgcac aagaagtacc tcacttgcat gattacatct
    18901 ataatgcaag agatgatcac tacgatactt ggaagtttaa agaattaaag gagtcaaacc
    18961 atccagtcct tttggcattc tctgaaaggt ggcacgatag tcgcttgact tctaaaagcc
    19021 ttgcagaatg tttacaatta accgaccttg atgaagaagt gaaatcgacc atcattcaat
    19081 taagacagtt cgaaaaatca gtcagaaatc ctttggctca cctgattaaa ccttttgatg
    19141 agcaagaact atatcgtaca actcaatttt cttctcaagc atttttagac cagattatct
    19201 tcttggcaaa ggtaattggt gttgagtatg atactgttaa ttttcactac gatacggtta
    19261 acaagcttat tataaagata cttgagtaat ttaaaaacag ttctgaga
//
LOCUS       DATROD010000026        17547 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.026, whole genome shotgun sequence.
ACCESSION   DATROD010000026 DATROD010000000
VERSION     DATROD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17547
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.026"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            131..2449
                     /locus_tag="VBC74_000902"
     CDS             131..2449
                     /locus_tag="VBC74_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEO6941865.1"
                     /translation="MLDELKAIIKSPKLWITMAGVALIPTLYNVIFLSSMWDPYGNTK
                     NLPVAVVNQDKSAKLNGKTISIGKDMEDNLSKNDSLDFHFTTAKRAEKELEKGHYYMV
                     ITFPKDLSRKATTLMTEKPERLNITYKTTKGRSFVASKMSETAANKLKDEVAESITGT
                     YTESVFKNMGSMKTGINKAADGSQELLNGSNKLQNGSQTLTSNLDVLASSSQTFSGGA
                     NKLNSGINLYTDGVGTLSNGLETLSDGVTAYTTGVHKLSEGSQKLDDKSQALVEGSEK
                     LTVGLQQLSQATQLKPEQERTLQNLSDGLKNLNQIITNLQSTATTDSDTNSKLFNFLS
                     TIESSTTALMNTAAADKQKQMTAVQSTSAFKSLTPEQQSQITSAVTGTPTSAETIAAN
                     ISSNIENMKTVLSEASSSAPSNNNSQNFQTLSGTANNLVLKAISDLDKIQKLPTATKQ
                     LYQGSQTLTKGITDYTNAVGQLRKGAETLDSKSNQLISGTQKASQGAQTLDSKSDQLR
                     DGAGQLASGSDRIADGSNKLAGGGHQLTDGLTELSGGVSQLSSSLGKAGDQLSMVSVN
                     KDNANAVSSPVTIKHEDYDSVDTNGVGMAPYMISVALMVVALSANVIFAKALSGKEPA
                     NRFSWAKNKLLINGFIATLAATILFFAVQFIGLKPDYPGKTYFIILLTAWTLMALVTA
                     LVGWDNRYGSFLSLLILLFQLGSSAGTYPIELSPKFFQTIQPFLPMTYSVSGLRETIS
                     LTGDVNHQWRMLVIFLVSSMILALLIYRKQED"
     gene            2516..2872
                     /locus_tag="VBC74_000903"
     CDS             2516..2872
                     /locus_tag="VBC74_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEO6941866.1"
                     /translation="MSTLTIIIATLTALEHFYIMYLETLATQSNMTGKIFSMSKEELS
                     YLPVIKLFKNQGVYNGLIGLFLLYGLYISQNQEIVAIFLINVLLVAVYGALTVDKKIL
                     LKQGGLPILALLTFLF"
     gene            3006..3749
                     /locus_tag="VBC74_000904"
     CDS             3006..3749
                     /locus_tag="VBC74_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6941867.1"
                     /translation="MTENWLHTKDGSDIYYRVVGQGQPIVFLHGNSLSSRYFDKQIAY
                     FSKYYQVIVMDSRGHGKSHAKLNTISFRQIAVDLKDILVHLEIDKVILVGHSDGANLA
                     LVFQTMFPDMVRGLLLNSGNLTIHGQRWWDILLVRIAYKFLHYLGKLFPYMRQKAQVI
                     SLMLEDLKISPADLQHVSTPVMVLVGNKDIIKLNHSKKLASYFPRGEFYSLVGFGHHI
                     IKQDSHVFNIIAKKFINDTLKGEIVEKAN"
     gene            3733..6273
                     /gene="mprF"
                     /locus_tag="VBC74_000905"
     CDS             3733..6273
                     /gene="mprF"
                     /locus_tag="VBC74_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEO6941868.1"
                     /translation="MKKLIEKVKSLTSVIKIVFFISVLVLIIVEMIHLKRTISVEQLK
                     SVFGQLSPMNLFLIILVGVIAVLPTTGYDFVLNGLLRTDKSKRYILQTSWCINTFNNL
                     SGFGGLIDIGLRMAFYGKKGQEKSDLREVTRFLPYLISGLSFISVIALIMSHIFHAKA
                     SVDYYYLVLIGASMYFPVIYWISGHKGSHYFGDMPSSTRIKLGIVSFFEWGCAAAAFI
                     IIGYLMGIHLPVYKILPLFCIGCAVGIVSLIPGGLGSFELVLFTGFAAEGLPKETVVA
                     WLLLYRLAYYIIPFFAGIYFFIHYLGSQINQRYENVPKELVSTVLQTMVSHLMRILGA
                     FLIFSTAFFENITYIMWLQKLGLDPLQEQMLWQFPGLLLGVCFILLARTIDQKVKNAF
                     PIAIIWITLTLFYLNLGHISWRLSFWFILLLLGLLVIKPTLYKKQFIYSWEERIKDGI
                     IIVSLMGVLFYIAGLLFPIRAHITGGSIERLHYIIAWEPIALATLILTLVYLCLVKIL
                     QGKSCQIGDVFNVDRYKKLLQAYGGSSDSGLAFLNDKRLYWYQKNGEDCVAFQFVIVN
                     NKCLIMGEPAGDDTYIREAIESFIDDADKLDYDLVFYSIGQKLTLLLHEYGFDFMKVG
                     EDALVNLETFTLKGNKYKPFRNALNRVEKDGFYFEVVQSPHSQELLNSLEEISNTWLE
                     GRPEKGFSLGYFNKDYFQQAPIALVKNAEHEVVAFANIMPNYEKSIISIDLMRHDKQK
                     IPNGVMDFLFLSLFSYYQEKGYHYFDLGMAPLSGVGRVETSFAKERMAYLVYHFGSHF
                     YSFNGLHKYKKKFTPLWSERYISCSRSSWLICAICALLMEDSKIKIVK"
     gene            complement(6352..7866)
                     /locus_tag="VBC74_000906"
     CDS             complement(6352..7866)
                     /locus_tag="VBC74_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019319700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEO6941869.1"
                     /translation="MTTLITTFQERFGDWTQSLIEHLQLSLLTLILATLIAIPLGIII
                     SHYKKISHVVLQITGIFQTIPSLALLGLFIPFMGIGTVPAVVALIIYALFPILQNTVT
                     ALMQIDANLIEAATAFGMTRWERLKKFELALSMPVIISGIRTASVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNPSLILIGAISSAVLAIIFSGLIGLLEKARLRTIAVSGILLLA
                     GLGLSYAPKWMPGTNTATITVAGKLGTEPDILINMYKELIEDQTDIKVKLKPNFGKTT
                     FLYQALKSGDIDLYPEFTGTITSSLLKNPPKVSNNPKQVYNLAKNGILKQDKLSLLSP
                     MAYQNTYAVAVKKDYAEANQLKNISDLKKLDKLKAGFTLEFKDREDGSIGLQKHYGLN
                     LDISTLEPALRYQAINSKDVNIIDAYSTDSELIQYQLQILKDDKHLFPPYQGAPLLRQ
                     DTIKKYPQVKKALNKLAGHITEKEMQEMNYQVAVKHKSAATVAKQYLKAHHIIK"
     gene            complement(7869..8591)
                     /locus_tag="VBC74_000907"
     CDS             complement(7869..8591)
                     /locus_tag="VBC74_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6941870.1"
                     /translation="MISFENVSKSYGDHTIIDNISCHIQRGEFFVLVGASGSGKTTIL
                     KMINRLIEPSQGAITLDGENITSLDLRQLRLETGYVLQQIALFPNLTVGENIELIPEM
                     KGWSKGDQKKAASDLLDKVGLPAKDYFNRYPHELSGGEQQRIGILRAIVAKPKVLLMD
                     EPFSALDPISRRQLQDITKQLQSELGITLVFVTHDMKEAMRLADRICVIKEGKIVQLD
                     RPEIIQNNPSDQFVRTLFEEEN"
     gene            complement(8813..9646)
                     /locus_tag="VBC74_000908"
     CDS             complement(8813..9646)
                     /locus_tag="VBC74_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEO6941871.1"
                     /translation="MKFYIIDDDPTITMILQDIIEEDFNNTVVRVNNVSSKAYNELLI
                     ADVDIVLIDLLMPILDGVTLVQKIYKQRSDLKFIMISQVKDNDLRQEAYKAGIEFFIN
                     KPINIIEVKSVVKRVTDTIEMQKKLNTIQNLLENTPSYQKPITTSNLTKIRSILSYLG
                     ITSETAYTDILNICELLLKQELNFAQFDFQKELSIDEHQQKIILQRIRRAVKKAMINM
                     AHLYIDDFENELTLQYANALFGFQNIHNEAQLIQGKSMYGGKISLKRFFDELILQSKT
                     F"
     gene            complement(9643..10938)
                     /locus_tag="VBC74_000909"
     CDS             complement(9643..10938)
                     /locus_tag="VBC74_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEO6941872.1"
                     /translation="MLDILSEKHLERRQRIIISAIAIALAAQINISILADGFIMTLSL
                     FILPVFLYFNDDINPILLCLGITFASPIFRGIILSIAGEAEIHQIIEFVLTDMAFYIC
                     YGITFYTIYWHRSYRNKGTFFFSIIICDYFANLVEISFLIKFNNYTITIFATLFAIAL
                     LRAFISCAVAYTYSYLSLLLQKDDHERRYYYFMWSTSAVKSEVYFMQKNIIEIENIMK
                     NAYLLDKELSKYHLPKEYQHLSLDISRDVHEVKKDYQNIIKGLGTYFSVKNESTMALK
                     DIFQIVLSYTRSIIQFRHQDIIILENNKCNLIISNYYYLLTIISNIVLNAVEAIDKQK
                     KGTISVHTEELEDFIKIEISDNGPGIPDKMKHMIFKPGFSTKFDANGDIYRGIGLSHV
                     RILMEEQYQGTITVCPNQPNGTTFTLLFRKNRFKLGDNT"
     gene            11041..12039
                     /gene="argF"
                     /locus_tag="VBC74_000910"
     CDS             11041..12039
                     /gene="argF"
                     /locus_tag="VBC74_000910"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO6941873.1"
                     /translation="MKNLRNRSFLTLLDFSTAEVEFLLKLSEDLKRAKYAGIEQQKLV
                     GKNIALIFEKDSTRTRCAFEVAAHDQGAHVTYLGPTGSQMGKKETSKDTARVLGGMYD
                     GIEYRGFSQETVETLAEFSGVPVWNGLTDADHPTQVLADFLTAKECLHKPYKDIRFTY
                     VGDGRNNVANALMIGASIVGMTYHLVCPKELEPDPELLSKCQEIAKTTGASIEITADI
                     AEGVRDSDVLYTDVWVSMGEPDEVWKERIALLEPYRITQEMLNMTENPNVIFEHCLPS
                     FHNIDTKVGYDIYEKYGLKEMEVSDEVFEGPHSVVFQEAENRMHTIKAVMVATLGD"
     gene            12051..12977
                     /gene="arcC"
                     /locus_tag="VBC74_000911"
     CDS             12051..12977
                     /gene="arcC"
                     /locus_tag="VBC74_000911"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO6941874.1"
                     /translation="MAKIVVALGGNALGNSPEEQLRLVKHTAKSLVALIKKGHEIVVS
                     HGNGPQVGAINLGMNFAAESGQGTNFPFPECGAMSQGYIGYHLQQSLLNELRQEGINK
                     EVATIITQIEVDESDQAFSAPTKPIGTFYDKETSEKIAIEKGYTFVEDAGRGYRRVVA
                     SPEPKKIIEINSIKTLIENDTLVIAGGGGGIPVINKGGYEGIAAVIDKDKSSALLAGE
                     LAADQLIILTAVDYVYTQFGKENQKALTEVNENQMIDYVNQGEFAKGSMLPKVIACMS
                     FLDHNPKGIALITSLNGLEDALDGKLGTRITK"
     gene            13071..14624
                     /locus_tag="VBC74_000912"
     CDS             13071..14624
                     /locus_tag="VBC74_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEO6941875.1"
                     /translation="MPEKMTNSENKRSKGFRMPGAFTILFILTIFSVLATWWIPAGSY
                     SKLQFDTASSKLVVTDPNGKTVHVPATQTQLDKMNVKIKIKEFTSGAISKPVSVPNTY
                     KRLKQNPAGIGSVTTSMVNGTIEAVDIMVFIMVLGGMIGVVRKSGAFESGLLALTKKT
                     KGREFLLIFLVSLLMVLGGTLCGIEEEAVAFYPILVPIFLAMGYDSIICVGAIFLASS
                     VGTSFSTINPFSSVIASNAAGISFTEGLSWRTAGCIAGAIFVVVYLHWYAKKIKANPE
                     FSYSYEDRVEFNAKWGMTTNHTPSLFTIRQKIILSLFVISFPLMVWGVMSQGWWFPTM
                     ASSFLAITIIIMFLTATGANGIGERDVVDEFVNGASSLVGVSLIIGLARGINIILSQG
                     YISDTMLYTASKLASHVSGPVFIIVMMFIYFVLGFVVPSSSGLAVLSMPILAPLADTV
                     GIPRSVVVMAYQFGQYAMLFLAPTGLVMATLQMLDMKYSHWLKFVWPVVLFLLIFGGG
                     LLVLQVLIL"
     gene            complement(14734..16098)
                     /locus_tag="VBC74_000913"
     CDS             complement(14734..16098)
                     /locus_tag="VBC74_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6941876.1"
                     /translation="MLFQSKINYPKKSSIITQITLWYSSFIFILVIGVLIGSFFISKS
                     IAENKSKKNLEAKAVQMSQALAKGHRYEAFEDGIFYSVYDQNGKVIYSGFPKGFKRDL
                     DHQHKHKKKLSLFSMENRTFQYVDIPISGKNQWLRAIRTVDRLDKQLTELLFSLGIVL
                     PLMLIIITVGGYLILKRAFRPIQEITETAQFITQNEDYTKRIITKNNENELTELAAVI
                     NTMLASIESSFVREKQFNNDVSHELRTPVTVILSESEYGKNYAENLSEAKESFEVIHR
                     QSLSMKKLVEQLLELTKAENPLSIQLEPLNFSIMMKQLVSDSSRLLDNTPIHLDSQIE
                     DDLWIIGQQTLLKRLFDNLFSNAIKFTNNHISISLRQSDNQIVFSIKDNGLGISVDDQ
                     SKIWNRFYQVDSARTKDSQSGIGLGLSLVKQIATIHRAKIWVDSKPDDGSQFTLTFPK
                     LKKD"
     gene            complement(16082..16753)
                     /locus_tag="VBC74_000914"
     CDS             complement(16082..16753)
                     /locus_tag="VBC74_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6941877.1"
                     /translation="MKILVVEDEFDLNRSIVKLLKKQHYSVDSASNGEEALQFVSVAE
                     YDVIILDVMMPKMDGFTFLKLLRNKGSQVSILMLTARDAVEDRIAGLDFGADDYLVKP
                     FEFGELMARIRAMLRRTNRQVSSDDIQIQDITINLSTKQVWRNDNLIDLTAKEYEVLE
                     YLARHRDQVLSRHQIREHVWDYDYYGESNIIDVLIKNLRRKLDNNRDGSLIKTKRGLG
                     YVIPK"
     gene            complement(16838..17509)
                     /locus_tag="VBC74_000915"
     CDS             complement(16838..17509)
                     /locus_tag="VBC74_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO6941878.1"
                     /translation="MTNYKNNFKKFMSNKLYVGLASITLLGIGGAIGVSGAALAHNHH
                     SEHMSFEHKHEGHDKHHDHREKYSLKTLKNISTKIDYKTALETAKKEARSGFISQIEL
                     NTKGDTPFYEIKVIDNKIAKKYKIDANSGKILSTTSDNQLNSKDAQLKQPSKDIQTAL
                     QAIQAKYKNAKIKEISLANNKSNKLVYKVKLLDGNTPKIAQFDSDKASFIKANKLNTT
                     HKNHD"
BASE COUNT         5515 a         2717 c         3289 g         6026 t
ORIGIN      
        1 ccccttaata tttttgtaca tttttaaaca cagtgtccaa aaacgagatt tttatcttga
       61 aattatctat catcactgta taatatattt tatcaaaaaa atgaacaata tgtccaaaat
      121 aggagaaaaa atgttagacg aattaaaagc aatcataaaa agcccaaaat tatggattac
      181 gatggctggg gttgcactga ttccaacgct atataatgtt atctttctaa gctcaatgtg
      241 ggatccttat gggaatacta aaaatttacc tgtggcagtc gttaaccaag ataaatcggc
      301 taaattaaat ggaaaaacta tttcaattgg caaagatatg gaggacaatt tgtctaaaaa
      361 tgatagctta gatttccatt ttacaacagc taaaagagca gaaaaagaat tagaaaaggg
      421 tcactattat atggttatta cctttcctaa agatctttct agaaaagcga caactctcat
      481 gacagaaaaa ccagaaaggt taaatattac ttataaaact actaaggggc gtagttttgt
      541 agcttcaaaa atgagtgaga cagcagctaa taaactaaaa gatgaggtag cagaatctat
      601 tacagggact tatactgaat cagtttttaa aaatatgggt tctatgaaaa caggtatcaa
      661 taaagctgct gatgggagtc aagaattatt aaatggttct aataagctac agaatgggag
      721 ccaaaccttg acaagtaatt tagatgtttt agcaagctct agtcaaactt tttctggagg
      781 agctaataaa ctaaatagtg gaataaacct ttatacagat ggtgtgggaa ccctatctaa
      841 cgggttagaa acattatcag atggtgttac tgcttacaca acaggtgtgc ataagttgag
      901 tgaaggtagt caaaaattag atgataagtc tcaagcattg gtggaaggat cagaaaagtt
      961 gacggttggt ttacaacaat tgtcacaagc aactcaacta aaaccagagc aagaacgtac
     1021 tttgcagaac ctttcagatg gattaaagaa tctaaatcag attattacaa atttacaatc
     1081 gacagcaact actgatagcg atactaatag taagttattt aattttttat caactattga
     1141 atcaagtaca acggctttga tgaatacggc tgctgctgat aaacaaaagc aaatgactgc
     1201 ggtacaatca acgtcagctt ttaaaagttt aacccctgaa caacaatctc aaattacctc
     1261 agcggttact ggaacaccta cttcagctga gacaattgca gcaaatattt cttcaaatat
     1321 agaaaatatg aaaactgtgc ttagtgaagc atctagttca gccccttcta ataataactc
     1381 acaaaatttt caaactttgt caggaacagc taacaattta gtattaaaag ctatctcaga
     1441 tttagataaa attcaaaaat tgccaacggc tactaagcag ttatatcaag gtagtcaaac
     1501 cttgacaaaa ggaatcacgg attatacgaa tgctgtaggg caattacgca aaggtgcaga
     1561 aactttagat agtaaatcca atcaattgat ttcaggaaca caaaaagcaa gtcagggagc
     1621 tcaaacttta gatagtaaat cggatcaatt gagagatgga gcggggcaat tagctagtgg
     1681 ttctgataga attgcagatg gttctaataa gcttgctggg ggaggacatc agttgacaga
     1741 tgggttgaca gagttgtcag gtggtgtttc tcaattatca agttctttgg gtaaagctgg
     1801 ggaccagttg tccatggttt ctgtaaacaa ggataatgcc aatgctgtat ctagcccagt
     1861 cactatcaag catgaagatt atgatagcgt tgatacgaat ggtgttggaa tggcaccata
     1921 catgatttca gttgcgttga tggtggtagc tttatcagcc aatgttattt ttgcgaaagc
     1981 tttatctggc aaagagccag ctaatcgttt tagttgggct aaaaataaat tattaatcaa
     2041 tggattcatt gcaactctag cagcaactat cttatttttt gcagttcaat tcataggtct
     2101 taaaccagat taccctggaa aaacctactt tattatccta ttgacagcat ggactttgat
     2161 ggcattagta actgctttag tgggatggga taataggtat ggttccttct tgtcgttatt
     2221 aatattatta ttccagcttg gttcaagcgc aggaacttac ccaatagaat tgagtcctaa
     2281 gttctttcaa acaattcaac catttttacc gatgacttac tctgtttcag gattaaggga
     2341 gaccatctcg ttgacaggag acgttaacca tcaatggaga atgctagtaa tctttttagt
     2401 atcatcgatg atacttgctc ttcttattta tcgtaaacaa gaagattaat agaaagtatc
     2461 tagtgataga ctaacagtat gatatgctat gtcaaagtat ttaggaggag aagatatgtc
     2521 tactttaaca ataattattg caacattaac tgctttggaa catttttata ttatgtattt
     2581 ggagacgtta gccacccagt caaatatgac tgggaagatt tttagtatgt ctaaagaaga
     2641 gttgtcatat ttacccgtta ttaaactttt taagaatcaa ggtgtataca acggcttgat
     2701 tggtctattc ctcctttatg ggttatatat ttcacagaat caagaaattg tagctatttt
     2761 tttaatcaat gtgttgctag ttgctgttta tggtgctttg acagttgata aaaaaatctt
     2821 attaaaacag ggtggtttac ctatattagc tcttttaaca ttcttatttt aatactactt
     2881 agccgttcga tttagttgaa cggcttttag taatcatttt tttctcataa tacaggtagt
     2941 ttaagtaatt tgtctttaaa aatagtataa tataactatg aattcaaaga gaggtgactt
     3001 tggttatgac tgagaactgg ttacatacta aagatggttc agatatttat tatcgtgtcg
     3061 ttggtcaagg tcaaccgatt gtttttttac atggcaatag cttaagtagt cgctattttg
     3121 ataagcaaat agcatatttt tctaagtatt accaagttat tgttatggat agtagagggc
     3181 atggcaaaag tcatgcaaag ctaaatacca ttagtttcag gcaaatagca gttgacttaa
     3241 aggatatctt agttcattta gagattgata aagttatatt ggtaggccat agcgatggtg
     3301 ctaatttagc tttagttttt caaacgatgt ttccagatat ggttagaggg cttttgctta
     3361 attcagggaa cctgactatt catggtcagc gatggtggga tattctttta gtaaggattg
     3421 cctataaatt ccttcactat ttagggaaac tctttccgta tatgaggcaa aaagctcaag
     3481 ttatttcgct tatgttggag gatttgaaga ttagtccagc tgatttacag catgtgtcaa
     3541 ctcctgtaat ggttttggtt ggaaataagg acataattaa gttaaatcat tctaagaaac
     3601 ttgcttctta ttttccaagg ggggagtttt attctttagt tggctttggg catcacatta
     3661 ttaagcaaga ttcccatgtt tttaatatta ttgcaaaaaa gtttatcaac gatacgttga
     3721 aaggagaaat tgttgaaaaa gctaattgaa aaagtcaaat cactgacttc tgtgattaaa
     3781 attgtatttt ttatatctgt tttagtgctt attattgttg aaatgattca tttgaaacga
     3841 actatttctg ttgagcaact aaagagtgtt tttgggcaat tatctccaat gaatcttttc
     3901 ttaattatcc ttgtgggggt tatcgctgtc ttaccgacaa ccggatatga ctttgtactg
     3961 aatggacttt tacgtacaga taaaagcaaa aggtatattt tacagactag ttggtgtatc
     4021 aacactttta ataacttgtc aggattcggt ggcttaatcg atattgggtt gcgcatggct
     4081 ttttatggta aaaaaggtca agagaagagt gacctaagag aagtgactcg ttttttaccc
     4141 tatcttattt ctggtctgtc atttattagt gtgattgcct taatcatgag ccatattttt
     4201 catgccaaag ctagtgttga ttactattat ttggtattaa ttggtgctag tatgtatttt
     4261 cctgttattt attggatttc tggtcataaa ggaagccatt atttcggaga tatgccatct
     4321 agtactcgta taaaattagg tattgtttct ttttttgaat ggggatgtgc agccgcagcg
     4381 tttataatta tcggttattt aatgggcatt catctaccag tttataaaat tttaccacta
     4441 ttttgtattg gttgtgccgt cgggattgta tcccttattc ccggtggatt aggaagtttt
     4501 gaattagttc tatttacagg gtttgctgcc gagggactac ctaaagaaac tgtggttgca
     4561 tggttattac tttatcgttt agcctactat attattccat tctttgcagg tatctatttc
     4621 tttatccatt atttaggtag tcaaataaat caacgttatg aaaatgtccc gaaagagtta
     4681 gtatcaactg ttctacaaac catggtgagc catttgatgc gtattttagg tgcattctta
     4741 atattttcaa cagcattttt tgaaaatatt acttatatta tgtggttgca gaagctaggc
     4801 ttggacccat tacaagaaca aatgttatgg cagtttccag gtttattgct gggggtttgt
     4861 tttattctct tagctagaac tattgatcaa aaagtgaaaa atgcttttcc aattgctatt
     4921 atctggatta ctttgacatt gttttatctt aatttaggtc atattagttg gcgactatct
     4981 ttctggttta ttttactatt gttaggctta ttagtcatta agccaactct ctataaaaaa
     5041 caatttattt atagctggga agagcgtatt aaggatggaa tcattatcgt tagtttaatg
     5101 ggagttctat tttatattgc aggactacta ttccctatca gggctcatat tacaggtggt
     5161 agtattgaac gcctgcatta tatcatagca tgggagccga tagcattggc tacgttgatt
     5221 cttactctcg tttatttatg tttggttaag attttacaag gaaaatcttg tcagattggt
     5281 gatgtgttca atgtggatcg ttataaaaaa ctacttcaag cttacggtgg ttcttcggat
     5341 agcggtttag cctttttaaa tgataaaagg ctctactggt accaaaaaaa tggagaagat
     5401 tgcgttgcgt tccaatttgt aattgtcaat aataaatgtc ttattatggg ggaaccagcc
     5461 ggtgatgaca cttatattcg tgaagctatt gaatcgttta ttgatgatgc tgataagcta
     5521 gactatgacc ttgtttttta cagtattgga cagaagttga cactactttt acatgagtat
     5581 ggttttgact ttatgaaagt tggtgaggat gctttagtta atttagaaac gtttactctt
     5641 aaagggaata agtacaaacc tttcagaaat gccctaaata gagttgaaaa ggatggtttc
     5701 tatttcgaag ttgtacaatc gccacatagt caagagctac taaatagttt ggaagagatt
     5761 tctaatactt ggttagaagg acgtcctgaa aaaggtttct cactaggata ttttaataaa
     5821 gattatttcc aacaagcccc aatagctttg gtgaaaaatg ctgaacacga agttgttgct
     5881 tttgctaata ttatgccaaa ctatgaaaag agtattatct ctattgattt aatgcgtcac
     5941 gataaacaga aaattccgaa tggcgttatg gatttcctct ttttatcatt attctcttat
     6001 tatcaagaga agggatacca ctattttgat ttggggatgg cacctttatc aggagttggt
     6061 cgcgttgaaa caagttttgc taaagagaga atggcgtatc ttgtctatca tttcggtagt
     6121 catttctact catttaatgg tttacacaag tataagaaga agtttacacc attgtggtcg
     6181 gaacgttata tttcttgttc tcgttcgtcc tggttaattt gtgctatttg tgccctatta
     6241 atggaagata gtaaaattaa gattgttaaa taagctttat ttggcaatta aaaagagcat
     6301 gtcatgcgac atgctctttt taaatcattt aataccattg attgcttgaa tctactttat
     6361 aatatgatgt gcttttaaat attgtttagc tactgtagct gctgatttat gctttacagc
     6421 tacttggtag ttcatttctt gcatttcttt ttcagtgata tgaccagcaa gtttattgag
     6481 agcttttttt acttgagggt attttttaat cgtatcttgc cttagtagcg gtgctccttg
     6541 atatggcgga aatagatgct tatcgtcttt gagaatttga agttgatact ggattaattc
     6601 actatcggta gagtaagcgt caataatatt gacatcttta ctattgatag cttggtatcg
     6661 taaagcaggt tctaaggttg agatatcaag gtttaaacca taatgtttct gtaaaccaat
     6721 actcccatcc tctctatcct taaattctaa agtgaaacca gcttttagtt tatctaattt
     6781 ttttaggtcg ctgatatttt tgagttgatt agcttcagcg taatcttttt taacagctac
     6841 agcgtaggtg ttttgataag ccataggtga caaaagtgac aacttatctt gttttaaaat
     6901 accgtttttg gctaagttgt aaacctgttt agggttatta cttactttag gaggattttt
     6961 aagtaaactt gatgtaatag tacctgtaaa ctctgggtaa agatcaatat caccagattt
     7021 tagggcttga tataagaaag ttgtttttcc gaaattaggt ttaagcttaa ctttgatatc
     7081 tgtctgatcc tcaataagtt ctttgtacat attaattaaa atatctggtt cagttcctaa
     7141 cttccctgca acagttatcg ttgcagtatt tgttccaggc atccattttg gagcatagga
     7201 tagtcctaag cctgctagta aaagtatacc agaaacggct atggttcgta atctagcctt
     7261 ttctagtaat ccaattaaac cactaaatat aatagctaag acagcagaag agattgcccc
     7321 tatgagaatt agagaaggat tgttgcgatc aatccctaac aaaataaatg atccaaggcc
     7381 accagctccg ataagactag ctaatgtagc tgtaccaata atcataacag aggcagttct
     7441 aatgcctgag atgataacag gcatacttaa agctaactca aactttttaa gcctttccca
     7501 acgtgtcatt ccaaaagcgg ttgctgcctc aatgagatta gcatcaattt gcatcagtgc
     7561 tgttacagta ttttgtaaaa ttggaaatag agcgtaaata attagtgcaa cgacagctgg
     7621 tacagttcca attcccataa aaggtataaa taaaccaagc aaggctaaag aaggaattgt
     7681 ttgaaatatc ccagttatct gaaggacaac atgactgatt tttttataat gactaatgat
     7741 gataccgagt ggaatagcaa ttaaagttgc taaaataagt gttagtagtg atagctggag
     7801 gtgttcaata agactttgag tccaatctcc aaaacgctct tgaaaggtag tgattagtgt
     7861 tgtcatactc agttctcctc ttcaaatagg gttctaacaa attggtcaga gggattattt
     7921 tgaattattt ctggtctatc caattgcact atcttacctt ccttaataac acaaattcta
     7981 tctgcaagac gcatagcttc cttcatgtca tgggtgacaa acactaatgt aatacccaat
     8041 tcagactgta attgtttggt gatatcttgt aattgacgtc ttgagatagg gtctaaagca
     8101 gaaaatggct catccataag tagtacctta ggtttagcaa cgattgctcg taggatacct
     8161 atgcgttgtt gttccccacc agagagctca tgaggatagc gattaaagta gtccttagca
     8221 ggtaaaccca ccttatcaag aaggtcgcta gcggcttttt tttggtcacc ttttgaccag
     8281 cccttcattt cagggataag ttctatattt tctccaactg ttaaattagg gaaaagtgca
     8341 atttgttgca agacataacc agtttctaag cgtaattgtc gcaaatcaag tgatgtaatg
     8401 ttttccccat ctaaagtaat agcgccttgg cttggttcga tgagacgatt aatcatcttg
     8461 aggatggtag ttttaccact gccactggcc cccacaagga caaagaattc tcctctttga
     8521 atatggcaac taatattatc aatgatggta tgatcaccat atgatttact aacattttca
     8581 aatgaaatca tgtaatttac tttctagtgc gtaacgagtg taggtcgtat gatattaggg
     8641 ttatattagc ataaagtaga cattcaaaca actatctgac atttaaattg tcagaaacga
     8701 taaactagaa gcaaaaacaa acaccttgcg gtagacaagg tgtttgttgg agatttatga
     8761 aaagaaaagt tttaggatgg ttatagtata taaggccatg cttaaacaag tcttaaaacg
     8821 ttttactctg taaaataagt tcatcgaaaa aacgtttaag tgaaatttta ccaccataca
     8881 tgctttttcc ttgaataagt tgtgcctcat tatggatatt ctgaaatcca aacaatgcat
     8941 tagcatattg cagtgttaat tcattttcaa aatcatcaat gtacagatga gccatattta
     9001 tcatagcttt tttaacggca cgacgaatac gttgtaaaat gattttttgt tggtgttcat
     9061 cgatagaaag ttctttttga aaatcaaatt gggcaaaatt taattcttgt tttaataaaa
     9121 gttcacagat gttaagaata tctgtgtagg ctgtctctga agtaatacct aaataactga
     9181 gtattgaacg gattttagtg aggttactag tcgttatagg cttttgatat gatggcgtat
     9241 tttctaggag attttggatt gtatttaatt ttttttgcat ttcgatagta tcagtgacac
     9301 gtttcacgac gctttttact tcaataatat taatgggttt attaataaaa aattcgattc
     9361 ctgctttata tgcttcttgt cgtaaatcat tatctttgac ttgggaaatc ataataaatt
     9421 ttaaatcgga acgctgttta tagatttttt ggactaatgt tacaccgtct aatattggca
     9481 ttaataaatc aatcaatacg atatcaacat ctgcaatcag caattcgttg tatgctttac
     9541 tggagacatt attcactctg actactgtat tattgaaatc ttcttcaata atatcttgta
     9601 aaatcatagt gatagtgggg tcatcatcaa taatatagaa tttcacgtat tatctcctaa
     9661 cttaaatcga tttttacgga ataacagtgt aaatgttgtt ccatttggct gattaggaca
     9721 aacagttatc gttccttgat attgctcttc cattaaaata cgcacgtggg atagcccgat
     9781 tcctcgatag atatcaccat tagcatcaaa tttagtcgaa aatccaggtt tgaaaatcat
     9841 gtgtttcatt ttatcaggta ttcctggtcc gttatcacta atctcaattt taataaaatc
     9901 ttctaattct tctgtgtgaa cactgattgt accctttttt tgtttatcaa tagcttctac
     9961 tgcatttaaa acgatattgg aaataatggt tagtaaatag taatagtttg atattatcaa
    10021 attacattta ttattttcga gaataatgat atcttgatgc cgaaattgaa taatactcct
    10081 agtataagat aaaacaattt gaaagatatc tttcaatgcc atagttgatt cattttttac
    10141 actaaaatag gttcctaagc ctttaataat attttggtaa tctttcttaa cttcgtgaac
    10201 gtcccgcgaa atatctagag agaggtgttg gtattcttta ggtaggtgat atttggataa
    10261 ctctttatct aataaataag catttttcat gatgttttca atttcaatga tatttttttg
    10321 cataaaataa acctcgcttt taacggctga agttgaccac ataaaataat agtatcgacg
    10381 ttcgtgatcg tctttttgta agagaagtga tagataacta taagtataag ccacagcgca
    10441 actaataaaa gctcgcaaaa gtgctatggc aaataaagta gcgaatatag ttatagtata
    10501 attgttaaac tttatgagaa aactaatttc tacaaggttg gcaaaataat cacaaataat
    10561 gatagaaaag aaaaaagtac ctttattacg gtaggaacga tgccaataaa tagtataaaa
    10621 ggtgatgcca taacaaatat aaaacgccat atcagttaaa acgaattcaa ttatttgatg
    10681 aatctcagcc tcgccagcga tacttaatat gattcctctg aagataggag aagcaaaagt
    10741 aattcctaga cagagaagaa ttggattaat gtcgtcatta aaatataaaa agacaggcaa
    10801 aatgaacaaa gataaggtca taataaaccc atcagcaagt attgagatat tgatttgagc
    10861 agctaaggcg atagctattg cagagataat aatacgctgc cttctttcta gatgtttttc
    10921 tgataatata tctagcatat aaatgtcctt aaagtttttt tgaagatttt tgatgaattt
    10981 tgattatttt tgaaagcgct atctacaata agagtataaa aaaaaaggag gcaataagca
    11041 atgaaaaatt tacgtaaccg tagttttctt actttattgg attttagtac agcagaagtt
    11101 gaatttcttt taaaactttc agaagattta aaacgtgcta aatacgcagg aattgaacaa
    11161 caaaaacttg ttggtaaaaa tatcgctttg atttttgaaa aagattctac acggacacgt
    11221 tgcgcttttg aagttgctgc tcacgatcaa ggagctcatg ttacttattt aggtccaaca
    11281 ggcagtcaga tgggtaaaaa agagacctca aaagacactg cgcgtgtgct aggtggtatg
    11341 tatgatggta ttgagtatcg tggattttct caagaaacag ttgagacttt agccgaattt
    11401 tcaggagttc ctgtgtggaa tggtttaacc gatgctgatc atccaacgca agtactagcc
    11461 gattttctga ccgctaagga atgcttacat aagccctata aagatattcg ctttacgtat
    11521 gtgggtgatg gacgtaacaa tgtcgctaat gccttgatga tcggtgcttc tattgtaggc
    11581 atgacttatc atttggtttg tcctaaagaa ttagagccag atcctgaact actctctaaa
    11641 tgtcaagaaa ttgcgaaaac tactggtgct tctattgaaa tcactgctga tattgcagaa
    11701 ggcgtcagag attcagacgt cctatataca gatgtttggg tttcaatggg agaaccggat
    11761 gaagtttgga aagaaagaat agctctttta gaaccctatc gtattaccca agaaatgctt
    11821 aatatgacag aaaatccaaa cgttattttc gagcattgct tgccctcatt tcataatata
    11881 gatactaaag ttggatacga catttatgaa aaatatggtt tgaaggaaat ggaagtatca
    11941 gatgaagttt ttgaaggccc tcactcagtt gttttccaag aagctgaaaa tcgtatgcat
    12001 actattaaag cagttatggt agcaacttta ggtgattagg aggaattttc atggcaaaaa
    12061 ttgttgttgc actaggagga aatgccttag gaaattctcc agaagaacaa ttaagattag
    12121 taaaacatac tgcaaaatca ttggtggcct tgataaaaaa gggacacgaa attgttgtaa
    12181 gtcatggtaa tggaccacaa gttggtgcca ttaatttagg aatgaatttt gcggctgaga
    12241 gtggtcaagg aactaatttt ccttttccag aatgtggagc aatgagccaa ggatacatag
    12301 gctatcatct ccaacagagc cttttaaatg aattacgtca agagggaata aataaggaag
    12361 ttgctacaat tattacccaa attgaggtgg atgaatcaga ccaagcattt agcgcaccta
    12421 ccaagcctat aggcaccttt tatgataagg agacatcaga aaagattgct atagagaaag
    12481 ggtatacttt cgtagaagac gctggacgag ggtatcgtcg tgttgttgct tcacctgaac
    12541 ctaaaaaaat catcgaaatt aatagcatta aaacacttat tgaaaatgat accctagtta
    12601 tcgcaggtgg aggtggaggc atccctgtca ttaataaggg aggttatgaa ggtattgcag
    12661 ctgttattga taaagacaaa tctagtgcac tgttagcagg agaactagct gcggatcaat
    12721 tgattattct cactgcagtt gattatgttt atactcaatt tggtaaagaa aatcaaaaag
    12781 ctttaacaga ggttaatgag aatcaaatga ttgattacgt taaccaaggt gagtttgcaa
    12841 aaggaagtat gttacctaaa gtaatagcct gtatgagttt cttagatcac aatccgaaag
    12901 gaatagcctt aatcacctca ttaaatggtt tagaagatgc attagatggg aaattaggaa
    12961 cacgtataac taagtaatct cagataagtg caaagaaatg attatttaac cttcatcttt
    13021 gcactttttc tgatgaagtc aatctatttt tattatagag gaggcgtgtc atgccagaaa
    13081 aaatgacaaa cagtgaaaac aaaaggagta aaggttttcg aatgccaggt gcctttacaa
    13141 tattattcat tttaacaatc ttttcggttt tggctacatg gtggatacct gctgggtcct
    13201 attcaaaatt acaatttgat acagcatcat ccaaactagt agtaacagat ccaaatggta
    13261 agacagttca tgttccagca acgcaaacac agttggataa gatgaatgtc aaaattaaaa
    13321 ttaaagaatt tacttctggt gctattagta agccagtttc agttccaaat acttacaaac
    13381 gtttgaagca aaatccagca ggcattggct ccgtgacgac tagtatggta aatggaacca
    13441 ttgaagctgt tgatattatg gtatttatca tggtacttgg aggcatgata ggagtagtta
    13501 gaaagagtgg cgcttttgag tctggtcttc tagctttaac aaagaaaacc aaaggtagag
    13561 agtttttatt gatattttta gtttccttat tgatggtttt aggcggtact ctatgtggta
    13621 ttgaagaaga agcagttgct ttttatccta ttttagttcc aatcttttta gccatgggtt
    13681 atgattctat catttgtgta ggtgctattt tcttagcaag ttctgttggg acatcatttt
    13741 caacaattaa tccgttttct tctgttattg cttcaaatgc agctggtatt agtttcacag
    13801 aaggcttatc atggcgtaca gcagggtgta ttgcaggtgc tatttttgtt gtggtgtatc
    13861 tacattggta tgctaaaaaa ataaaagcga atccagagtt ttcatattcc tatgaagata
    13921 gagtagaatt taatgctaaa tggggcatga caactaacca tacaccctca ctctttacta
    13981 ttagacaaaa aattatttta agtttatttg ttatttcatt tcctttgatg gtttggggag
    14041 taatgtcaca gggatggtgg tttccaacca tggcttcttc ctttttagct attacaatta
    14101 ttattatgtt tttaacagca actggggcaa atggtattgg agaaagggat gttgttgatg
    14161 agtttgttaa tggtgcatca agccttgttg gggtttcatt aatcattggt ttagcgcgtg
    14221 gtatcaatat tattttgagc caaggttata tatcagatac gatgctttac acagcttcaa
    14281 aattagcttc acatgttagt ggccctgtct ttatcattgt tatgatgttt atctactttg
    14341 tgttaggatt tgttgttcca tcctcgtcgg gtttagcagt actttcaatg ccaattctag
    14401 cacctcttgc agatactgtt gggattcctc gttcagtagt agtaatggct tatcaatttg
    14461 ggcagtatgc aatgttgttt ttagcaccta ctggactagt tatggcaacc ttgcagatgt
    14521 tagatatgaa gtactcccat tggctaaaat ttgtgtggcc ggttgtttta ttcctcctaa
    14581 tatttggtgg cggtttgtta gttctacaag ttttaatact ttaaagattt cagctattag
    14641 tctcaaaaat atctgaagag atataataaa ttgagtttaa gttaaataaa aaaaacgata
    14701 ggtgtaatct atcgtttttt ttatttcctt tctctagtct ttcttcaatt ttggaaaggt
    14761 gagcgtaaac tgactgccat catcaggttt agaatcaacc catattttag cacggtgtat
    14821 tgtagcgatt tgttttacta aagatagccc taaaccaatt ccagactgac tatccttggt
    14881 tcgagcagag tccacttgat agaaacggtt ccaaatttta ctttggtcat caactgagat
    14941 acctagacca ttatctttaa tagagaagac aatttgatta tcggactgtc ttagactaat
    15001 agaaatatga ttgtttgtaa atttaatagc atttgaaaat agattatcaa aaagccgttt
    15061 gagtagtgtt tgttgaccta taatccaaag gtcatcttcg atttgagagt caaggtgtat
    15121 aggggtatta tctaataatc tactcgaatc ggatactagt tgcttcatca taattgagaa
    15181 attaagaggt tctaactgaa ttgataatgg attttctgcc ttagtcaatt ctagaagttg
    15241 ctcaactaat tttttcattg atagtgactg acggtgtata acctcaaagg attcttttgc
    15301 ttcacttaaa ttttctgcgt agttttttcc gtattcactt tcagaaagta taactgttac
    15361 aggagtccgt agttcatgag aaacatcatt gttaaattgt ttttcccgaa caaatgatga
    15421 ttcaatagaa gccaacatag tattgataac agctgctagc tcagttaatt cattttcgtt
    15481 atttttagtg ataattcgct tagtatagtc ttcattttgg gtaataaatt gagcggtttc
    15541 agtaatttct tgaatcggtc taaaagcgcg ctttaaaatt aaatagccac caacagttat
    15601 tataatcaac ataagaggaa gaacgatacc caaactaaat aacaattcag ttaattgctt
    15661 atccagtctg tcaacagtac ggatagcacg taaccactga tttttcccag aaattggaat
    15721 atcaacatac tgaaaggtac gattctccat ggaaaataga gataatttct ttttatgttt
    15781 atgttgatgg tctaaatccc ttttaaatcc ttttggaaaa ccagaataga taacttttcc
    15841 attttggtca tagactgaat aaaatattcc atcttcaaaa gcctcatacc gatgaccttt
    15901 agctaacgct tgactcattt gcacagcttt agcttctaaa ttttttttag atttgttttc
    15961 agcaatgctt tttgaaataa aaaatgaacc aattaataca ccgataacaa ggataaaaat
    16021 aaaagagctg taccagaggg taatttgagt gatgatgcta gactttttag ggtaatttat
    16081 tttactttgg aataacatat cctaaacctc gtttagtttt tattagtgat ccatctcgat
    16141 tgttatctag ttttcgacga aggtttttga taaggacatc aataatattg gactctccat
    16201 aataatcata atcccaaacg tgttcacgaa tttgatgacg agagaggact tggtctctgt
    16261 gacgtgccaa atactcaagg acttcgtatt ccttagctgt taaatcaatc aaattgtcgt
    16321 ttctccaaac ttgctttgta gataaattga ttgttatatc ttgaatttga atatcatcag
    16381 aagatacttg tctatttgta cgtcgtaaca tagctcgaat tcgggccatg agttctccaa
    16441 attcaaatgg ttttacaagg taatcatccg caccaaaatc tagacctgcg atacgatctt
    16501 caacggcatc tcgagcagtc aacataagaa tagatacttg acttcctttg ttgcgaagca
    16561 gttttaaaaa ggtgaagccg tccatttttg gcatcattac atctaagata atcacatcat
    16621 attctgccac tgaaacaaat tgtaaggctt cttcaccatt tgaagcactg tcaacactgt
    16681 agtgttgctt ttttaaaagt tttacaatac tacggttcaa gtcaaactca tcttcaacaa
    16741 caaggatttt cattttttgg cctccgtatg gcttttttcc attatatcac aaaagggaat
    16801 ggagtactga ggtcctattc cctttaatac actagtatta gtcgtgattt ttatgagttg
    16861 tattaagctt atttgccttt ataaatgatg ctttatcact atcaaattgg gcaatcttag
    16921 gagtatttcc atcaagtagt tttactttat aaactagctt atttgatttg ttattcgcaa
    16981 gagaaatttc ttttattttt gcatttttat attttgcttg aatagcttgt aatgcagtct
    17041 gaatatcttt acttggttgt tttaattggg catcttttga attgagttga ttatctgatg
    17101 ttgttgaaag gatcttccca gaattagcat caattttata ttttttagct attttattat
    17161 caataacttt gatttcataa aaaggtgtat cacctttggt atttaattca atttgtgaaa
    17221 taaagcctga gcgagcttct tttttggcag tttctaaggc agtcttataa tcgatttttg
    17281 tcgagatatt ctttaatgtt tttagtgagt atttttctct atgatcgtga tgtttatcgt
    17341 gtccttcatg cttatgttca aaagacatgt gttctgagtg atggttatgc gctaatgcag
    17401 ctccacttac tcctatagct cccccaatac caagtagcgt aatggaagct aaccctacgt
    17461 ataatttatt gctcatgaat tttttaaagt tgtttttata gtttgtcata ctgacttcct
    17521 tctttctttt ttcaaacctt ggattag
//
LOCUS       DATROD010000027        17424 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.027, whole genome shotgun sequence.
ACCESSION   DATROD010000027 DATROD010000000
VERSION     DATROD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17424
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.027"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..2716)
                     /locus_tag="VBC74_000916"
     CDS             complement(<1..2716)
                     /locus_tag="VBC74_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEO6941879.1"
                     /translation="MASTNNVSDTNTRFAKEELEEVFEELGTTQGGLSDEEVAVRQKK
                     YGLNLLSEVKQESIILLFLKNFTSLMAILLWVGGFVAIVSNSLELGLAIWMVNVINGI
                     FSFIQEYRASQATQALKKMLPSYSRVLRKSSEEKVLSEQLVPGDIVLIEEGDCISADG
                     RLIKTTDLQVNQSALTGESNPIYKDSNVENYQSKTLIECDNMVFAGTTVSSGSATMVV
                     TAIGMQTQFGQIADLTQGMKSEKSPLQRELDRLTKQISIISITVGIIFFLAATFFVKE
                     PVSKSFIFALGMIVAFIPEGLLPTVTLSLAMAVQRMAKEHALVKKLSSVETLGATSVI
                     CSDKTGTLTQNEMTVNHLWQNGKSYQVTGLGYAPEGQILFEGDNICFGNSDRGDLEKL
                     IRFAHLCSNAQVLPPNDDRSTYTVLGDPTEACLNVLLEKSGINIQENRKFAPRLKELP
                     FDSVRKRMTTIHSLGGDEKDKKISITKGAPKEILDLSDYVLSDGKVIPLNKEERNKIQ
                     LANDTFAKDGLRVLAVSYCDIEGFSKEQWTQENLEQHMVFIGLIAMSDPPREGVREAI
                     DKCHAASIRIIMVTGDYGLTALSIAKNIGIIRNDDAKVISGLELSEMTDSQLKKELSG
                     EVVFARVAPEQKYRVVTILQEMGEVVAVTGDGVNDAPALKKSDIGVAMGVTGTDVAKE
                     SADMILTDDHFASIVHAVEEGRAVYQNIKKFLTYIFNSNTPEAVPSAFFLFSKGFIPL
                     PLTVMQILAVDLGTDMLPALGLGVEPPETDVMNRPPRRLTDRLLDKGLLIKSFLWYGT
                     IESVLAMGGFFWAHYLRYGNFTFFVANGIPYREATTMTLGAIIFSQIGMVMNSRTSYQ
                     SIKTLSIFGNKLINFGIIMEILAFLVLVYVPLFHNLFNTASLGLSHWLY"
     gene            complement(2865..3302)
                     /gene="msrB"
                     /locus_tag="VBC74_000917"
     CDS             complement(2865..3302)
                     /gene="msrB"
                     /locus_tag="VBC74_000917"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEO6941880.1"
                     /translation="MKETQEELRQRIGHTAYQVTQNSATEHAFTGKYDDFFEEGIYVD
                     IVSGEVLFSSLDKFQSGCGWPAFSKPIENRMVTNHQDHSHGMHRIEVRSRQADSHLGH
                     VFNDGPVDAGGLRYCINSAALDFIPYDQMAKRGYGDYLSLFDE"
     gene            complement(3302..3766)
                     /locus_tag="VBC74_000918"
     CDS             complement(3302..3766)
                     /locus_tag="VBC74_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6941881.1"
                     /translation="MIRRAKEKDLPAIAELLKQILVLHHGVRPDIFHTRGSKFSKEQL
                     KEMLMDESRPIFVYESDEGKVVAHLFLQLQEKRDLPRKSFKTLYIDDLCIDEEVRGQQ
                     IGQKLMDFARQYAKKHGCYNITLNVWNDNQRAVSFYEKLGFKPQQTQMEEIL"
     gene            complement(3775..4434)
                     /locus_tag="VBC74_000919"
     CDS             complement(3775..4434)
                     /locus_tag="VBC74_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO6941882.1"
                     /translation="MTMVKREDVILNATENMVYHKLKNDPSGHDWFHIVRVRNLAVEL
                     AHKEGANTFICQMAALLHDIIDDKICQDSKQASYELTQWLYSQDLAIAEVEHILDILE
                     NISFKAGTGLTMKTLEGQIVQDADRLDAMGAIGIARTMAYSGSKGRLIHDPNLKPREN
                     LTLEEYRNGQDTAIMHFYEKLLKLKDLMNTKQGKMLAQKRHDFLELYLAEFYAEWNGK
                     G"
     gene            complement(4534..7167)
                     /locus_tag="VBC74_000920"
     CDS             complement(4534..7167)
                     /locus_tag="VBC74_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO6941883.1"
                     /translation="MKGQKIIALAGLVLSCHFALTACHTQEHKNSYHIKTKQVAKKKA
                     NKKKVSVKESHKKRKGVAGVDFPTDDGFLLTKDSKILSHTDSGIVVAHGNHSHFIFYS
                     DLKGSKFSYLIPNANAKTNKNQAVRNFKAGAVAVNTLNDGYVFNPADIVSEDANGYVV
                     RHGDHFHYIPKASLSQQKQVQASRAVSRLGNQSNSHYRVNSSKIAGLHYPTSDGFLFN
                     GRGIKGTTPTGILVEHHNHLHFISFADLRKGGWGSIADRYQPQKKADSKKQSPSSKKP
                     RNENTLPKDIKDKLAYLARELHLDISRIRVLKTLNGEIGFEYPHDDHTHVIMAKDIDL
                     SKPIPNPHHDDEDHHKGHHHDESDHKHEEHEHTKSNKLSDENQKKLIYLAEKLGLNPN
                     QIEVLTSEDGSIIFKYPHDDHSHTIASKDIEIGKPIPDGHHDHSHAKDKVGMATLKQI
                     GFDDEIIQDILHADAPTPFPSNETNPEKMRQWLATVTKINIGQRTNPFQRFGLSLMPN
                     IEVLGIGFTPINDMTPVLQFKKLKQLWMTNTGITDYSFLDKMPLLEGLDISQNGIKDL
                     SFLTKYKQLSLIAAANNGITSLKPLAELPNLQFLVLSHNNISDLTPLSNLTKLQELHL
                     DHNNVKNLSALSGKKDLKVLDLSNNKSADLSTLKTTSLETLLLNETNTSNLSFLKQNP
                     KVSNLTINNAKLSSLDGIEESDEIVKVEAEGNQIKSLVLKNKQGSLKFLNVTNNQLTS
                     LEGVNNYTSLETLSVSRNKLKSLDIKTPNKTVTNLDFSHNNVPTSQLKLNEKNIPEAV
                     AKNFPAVVEGSMVGNGSLAEKAAMASKEDKQVSDNTNHQKNTEKSAQANADSKKENPK
                     THDEHHDHEETDHAHVGHHHH"
     gene            complement(7413..9245)
                     /gene="lepA"
                     /locus_tag="VBC74_000921"
     CDS             complement(7413..9245)
                     /gene="lepA"
                     /locus_tag="VBC74_000921"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEO6941884.1"
                     /translation="MNIEDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGFTIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRAEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGEVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGMVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYIAASIKTVAD
                     TRVGDTITLANNPAIDPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVNTTDGEMLE
                     VSNPSEFPDPTRVDSIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYEISEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDDKK"
     gene            complement(9381..9797)
                     /gene="ndk"
                     /locus_tag="VBC74_000922"
     CDS             complement(9381..9797)
                     /gene="ndk"
                     /locus_tag="VBC74_000922"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEO6941885.1"
                     /translation="MEQTFFMIKPDGVKRGFIGEVISRIERRGFSIDRLEVRHADADI
                     LKRHYAELTDRPFFPTLVDYMTSGPVIIGVISGEEVISTWRTMMGSTNPKDALPGTIR
                     GDFAQAPSPNQATCNIVHGSDSPESAAREIAIWFNN"
     gene            complement(9938..10075)
                     /locus_tag="VBC74_000923"
     CDS             complement(9938..10075)
                     /locus_tag="VBC74_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941886.1"
                     /translation="MVKGLDCSTLYLIISLTALVLLVIAGVSLSLILASLQGKQWRTY
                     P"
     gene            complement(10107..10367)
                     /locus_tag="VBC74_000924"
     CDS             complement(10107..10367)
                     /locus_tag="VBC74_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941887.1"
                     /translation="MLGHRCGVLVFCYTEINFQRMWGKAVIRLIQDVIQNLVGQANEI
                     TPVYQFDWETYILATKKYERHLEVCLLVENSNYFSDSKRMCR"
     repeat_region   10420..11248
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=10420..10456
                     /rpt_unit_seq="gttttggaaccattcgaaacagcacagctctaaaact"
     gene            complement(11348..12013)
                     /gene="csn2"
                     /locus_tag="VBC74_000925"
     CDS             complement(11348..12013)
                     /gene="csn2"
                     /locus_tag="VBC74_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HEO6941888.1"
                     /translation="MIKINFPILDEPLVLSNATILTIEDVSVYSSLVKHFYQYDVDEH
                     LKLFDDKQKSLKATELMMVTDILGYDVNSAPILKLIHGDLENQFNEKPEVKSMVEKLA
                     ATITELIAFECLENELDLEYDEITILELIKALGVKIETQSDTIFEKCFEIIQVYHYLT
                     KKNLLVFVNSGAYLTKDEVIKLCEYINLMQKSVLFLEPRRLYDLPQYVIDKDYFLIGE
                     NMV"
     gene            complement(12000..12341)
                     /gene="cas2"
                     /locus_tag="VBC74_000926"
     CDS             complement(12000..12341)
                     /gene="cas2"
                     /locus_tag="VBC74_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEO6941889.1"
                     /translation="MSYRYMRMILMFDMPTETVEERKAYRKFRKFLLSEGFIMHQFSV
                     YSKLLLNNTANNAMIGRLKVNNPKKGNITLLTVTEKQFARMVYLHGERNTSVANFDSR
                     LVFLGDSYDQD"
     gene            complement(12338..13207)
                     /gene="cas1"
                     /locus_tag="VBC74_000927"
     CDS             complement(12338..13207)
                     /gene="cas1"
                     /locus_tag="VBC74_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEO6941890.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLVFKDSYQTEMIHLSEIDILIME
                     TTDIVLSTMLIKRLVDENILVIFCDDKRLPTAMLMPYYARYDSSLQLSRQMSWIEDVK
                     ADVWTSIIAQKILNQSFYLGECSFFEKSQSIMNLYHDLESFDTSNREGHAARIYFNTL
                     FGNDFSREHDNPINAGLDYGYSLLLSMFAREVVKCGCMTQFGLKHANQFNQFNLASDI
                     MEPFRPIVDRIIYENRQSDFVKMKRELFSMFSETYSYDGKEMYLSNIVSDYTKKVIKS
                     LNSDGNGIPEFRI"
     gene            complement(13209..17321)
                     /gene="cas9"
                     /locus_tag="VBC74_000928"
     CDS             complement(13209..17321)
                     /gene="cas9"
                     /locus_tag="VBC74_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040090.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEO6941891.1"
                     /translation="MNKPYSIGLDIGTNSVGYSVVTDDYKVPAKKMRVLGNTDKEYIK
                     KNLIGALLFDGGNTASDRRLKRTARRRYTRRRNRILYLQEIFAEEMSKVDDSFFHRLE
                     DSFLVEDDKRGSKYPIFATMQEEKDYHEKFPTIYHLRKELADKKEKADLRLVYLALAH
                     IIKFRGHFLIEDDSFDVRNTDIQRQYQAFLEIFDTTFENNHLLSQNIDVEGILTDKIS
                     KSAKKDRILAQYPNQKSTGIFAEFLKLIVGNQADFKKHFNLEDKTPLQFAKDSYDEDL
                     ENLLGQIGDEFADLFSVAKKLYDSVLLSGILTVTDLSTKAPLSASMIQRYDEHREDLK
                     QLKQFVKASLPEKYQEIFTDSSKDGYAGYIEGKTNQGAFYKYLSKLLTKQEGSEYFLE
                     KIKNEDFLRKQRTFDNGSIPHQVHLTELKAIIRRQSEYYPFLKENLDRIEKILTFRIP
                     YYVGPLAREKSDFAWMTRKTDDSIRPWNFEELVDKEASAEAFIHRMTNNDLYLPEEKV
                     LPKHSLIYEKFTVYNELTKVRYKNEQGETYFFDSNIKQEIFDGVFKEHRKVSKKKLLD
                     FLAKEYEEFRIVDVIGLDKENKAFNASLGTYHDLKKILDKDFLDNPDNESILEDIVQT
                     LTLFEDREMIKKRLENYKDLFTESQLKKLYRRHYTGWGRLSAKLINGIRDKESQKTIL
                     DYLISDGRANRNFMQLIHDDGLSFKPIIDKAQAGSHSDNLKEVVGELAGSPAIKKGIL
                     QSLKIVDELVKVMGYEPEQVVVEMARENQTTNQGRRNTRQRYKLLEEGVKNLASDLNG
                     NILKEYPTDNQALQNERLFLYYLQNGRDMYTGEALDIDNLSQYDIDHIVPQAFIKDDS
                     IDNRVLVSSAKNRGKSDNVPSIDIVKARKAFWKKLLDAKLMSQRKYDNLTKAERGGLT
                     SDDKARFIQRQLVETRQITKHVARILDERFNNELDSKGRRIRKVKIVTLKSNLVSNFR
                     KEFGFYKIREVNDYHHAHDAYLNAVVAKAILTKYPQLEPEFVYGDYPKYNSYKTRKSA
                     TEKLFFYSNIMNFFKTKVTLADGTVVVKDDIEVNNDTGEIVWDKKKHFATVRKVLSYP
                     QVNIVKKTEIQTGGFSKESILAHGNSDKLIPRKTKDIYLDPKKYGGFDSPIVAYSVLV
                     VADIKKGKAQKLKTVTELLGITIMERFRFEKNPSAFLESKGYLNIRDDKLMILPKYSL
                     FELENGRRRLLASAGELQKGNELALPTQYMKFLYLASRYNELKGKPEEIEQKQEFVVQ
                     HVSYFDDILQIINDFSNRVILADANLEKINKLYQDNKENISVDELANNIINLFTFTSL
                     GAPAAFKFFDKIVDRKRYTSTKEVLNSTLIHQSITGLYETRIDLGKLGED"
BASE COUNT         5395 a         3421 c         2692 g         5916 t
ORIGIN      
        1 aatagagcca atgtgataaa cctaggcttg ctgtgttgaa taagttatgg aatagtggca
       61 cgtatactaa gactaaaaat gctaaaatct ccattattat accaaaatta attaatttat
      121 taccaaaaat agatagtgtt ttaatagatt gatagctcgt tctgctattc ataaccatac
      181 ctatctggct aaagataata gctccaagtg tcatagttgt cgcttctctg tacggtatgc
      241 cgtttgcgac gaaaaaagta aaattaccat aacgtaagta atgagcccag aaaaaaccac
      301 ccatagctaa tacggattca atagtgccgt accaaaggaa agattttata agtagccctt
      361 tgtccaataa gcgatctgtt agccgtcttg gaggacgatt cataacatct gtttctggtg
      421 gttccacgcc tagacctaaa gcgggaagca tatcggttcc caaatcaaca gcaagtattt
      481 gcattacagt caatggtaaa ggaataaaac ctttagaaaa aaggaagaat gccgacggaa
      541 ccgcctcagg agtattacta ttaaaaatat acgtcaagaa tttcttaatg ttttgataaa
      601 cagcccgccc ttcttccacg gcatgtacaa tagaagcaaa atgatcatct gtcagaatca
      661 tatcagctga ttctttagca acatcagtac cggtaacacc catggctaca ccaatatcgg
      721 atttttttag cgcaggcgca tcattgaccc cgtctcctgt aactgctact acttcaccca
      781 tctcctgtaa tatcgttact actcgatatt tttgttctgg tgctacacga gcaaaaacga
      841 cttcacctga caattctttt ttcaattggc tatccgtcat ttctgataac tctaaacctg
      901 aaattacttt agcatcatcg ttacgaataa taccaatatt tttagcgata ctcagtgccg
      961 ttaatccgta atctccagtc accataataa tacgaatgct tgctgcatga catttatcaa
     1021 tagcttcacg aacgccttca cgaggtggat cactcattgc tatcaaccct ataaacacca
     1081 tgtgttgttc caaattttct tgcgtccatt gttcttttga aaagccttca atatcgcagt
     1141 atgaaacagc taagacgcga agtccatctt tagcaaatgt atcattagcc aactgtattt
     1201 tattacgttc ttccttattt aaggggataa ctttgccatc agataagaca taatctgata
     1261 aatcaagtat ttctttcggc gcaccttttg tgattgaaat ttttttatcc ttttcatcgc
     1321 ctcctaagct atgaatagtt gtcatgcgtt ttctaaccga atcaaaaggt aactctttca
     1381 aacgtggtgc aaacttacga ttttcttgta tgtttatccc tgatttttcc aataaaacat
     1441 tcaaacaagc ttctgtcgga tctccaagta cagtataagt tgaacgatca tcatttggtg
     1501 gtaatacttg agcgttgcta cagagatgag caaaacgaat aagtttttct aagtctcctc
     1561 tatcactatt accaaagcaa atattgtcac cttcaaaaag aatctgtcct tctggagcat
     1621 aacccagtcc tgtcacttgg taactctttc cattttgcca caaatgattg acggtcattt
     1681 cattttgcgt taatgtacca gtcttatcag aacagataac tgaggttgct ccaagagtct
     1741 caactgaaga aagttttttg acaagtgcgt gttctttagc cattcgttgg actgccatag
     1801 ctagagaaag tgtaacggta ggtagtaaac cttctgggat aaatgcaacg atcatcccca
     1861 aggcaaagat aaatgatttt gacactggct ccttaacaaa aaaagtcgct gctaagaaaa
     1921 agataattcc tacagtaatt gaaatgatag aaatctgttt ggtcaaccga tcaagttctc
     1981 tttgaagagg gcttttttct gatttcatcc cttgtgttaa atcagcgatt tgaccaaatt
     2041 gggtttgcat tccaattgcc gtaacaacca ttgttgcgct accactggag actgtcgttc
     2101 ctgcgaaaac catattatca cattcaatca atgttttaga ctggtagttt tcaacattgc
     2161 tatctttata aataggattt gattcccctg tcaaagctga ttggtttact tgcaaatctg
     2221 ttgttttaat caagcgacca tctgctgaaa tacaatctcc ttcttcaata agaacaatat
     2281 ctcctgggac tagttgctct gataatacct tttcttctga gctttttcta agaacacgtg
     2341 aataagatgg taacattttc ttgagagctt gtgttgcttg gcttgcacgg tattcctgaa
     2401 tgaaactaaa aataccatta atcacattaa ccatccaaat ggcaagacct agctctaagc
     2461 tattagaaac aatagccaca aatcctccaa cccataatag tattgccata agactagtaa
     2521 aatttttcag aaacaataaa ataattgatt cttgctttac ttcagataac aggtttaaac
     2581 catatttttt ctgacgtaca gcaacctctt catctgataa accaccttga gttgtcccta
     2641 attcttcgaa aacctcttct aattcctctt tagcaaaacg tgtgtttgta tctgaaacat
     2701 tatttgtaga agccatattc tagtctcctt ctctaaaacg ttttcatttt tgatacctat
     2761 attatactct tttttcagaa aataaactga tgaagaatac acctgccacc aattcttttt
     2821 accatacata aaaaacgact taagatatac ccttaagtcg tctcttattc atcaaaaagt
     2881 gataagtagt ccccatagcc tctctttgcc atctggtcat aaggaataaa atctaaggca
     2941 gctgaattga tacaatagcg taagccacct gcatcaaccg gtccatcatt aaatacatgc
     3001 cctaaatgag agtcagcttg acgactccta acctcaatac gatgcattcc atgagagtga
     3061 tcttgatgat ttgtcaccat tcgattctca attggttttg aaaatgctgg ccaaccacaa
     3121 ccagattgaa atttatctaa agaagagaat aaaacctctc cactgacgat atctacatat
     3181 atcccctctt caaaaaaatc atcatacttc ccagtaaaag catgttccgt agctgaattt
     3241 tgggttactt gataagctgt atgtccgata cgctgacgta attcttcttg tgtttctttc
     3301 attatagtat ctcctccatt tgagtttgtt gaggtttaaa acctaatttt tcataaaaag
     3361 agaccgctct ctgattatca ttccaaacat ttaaagttat attataacac ccatgtttct
     3421 tagcatattg tcttgcaaaa tccatcaact tttgtccaat ttgctgacct cttacttcct
     3481 catcaatgca caaatcatct atatagagag ttttaaagga ctttcgaggt aagtcacgtt
     3541 tttcttgtaa ttgaagaaac aagtgggcaa caactttacc ctcgtctgat tcataaacaa
     3601 agataggcct tgattcgtcc atcagcattt ctttcaattg ttctttagaa aacttacttc
     3661 ctcttgtatg aaaaatatct gggcgaaccc catgatgtaa cactaaaatt tgtttcaata
     3721 gctctgcaat agcaggcaaa tctttttctt tcgctctacg aatcatatct actcctaacc
     3781 tttaccattc cattcagcat aaaattcggc taaatatagt tctaaaaaat catgacgttt
     3841 ttgagcaagc attttccctt gttttgtatt cataaggtct tttaacttta ataatttttc
     3901 gtaaaaatgc atgatagctg tatcctgacc attacgatac tcttctaaag tgaggttttc
     3961 tcgtggtttt aagttagggt catgaattaa acgtccttta ctccctgaat aagccatcgt
     4021 tcttgcaata ccaatagccc ccatagcatc taggcgatct gcatcttgaa caatttgacc
     4081 ttccaatgtt ttcatagtca atccagttcc tgctttgaat gaaatatttt caagaatatc
     4141 aaggatatgt tctacttctg ctatggcaag atcttgacta tataaccatt gagtcaattc
     4201 atacgacgct tgcttactat cttggcagat tttgtcatca ataatatcat gcaaaagtgc
     4261 agccatttga caaataaagg tattagcacc ttctttgtgt gctaattcta ctgcaagatt
     4321 tcttactcta acaatatgaa accagtcatg gccacttgga tcatttttta atttatggta
     4381 aaccatattt tcagttgcat ttaaaatcac atcttctctt ttaaccatag tcatattata
     4441 gctaattttc taaaaaaata aatgatttag catataaatg caaaatcctc caagaaattc
     4501 ttgaaggatt ttatatatta gctccgtaaa agattaatgg tgatgatgac ctacatgtgc
     4561 atgatctgtt tcttcatggt catgatgttc atcatgtgtt ttagggtttt cttttttact
     4621 atcagcattc gcttgagcag atttttcagt gtttttttgg tgattagtat tatctgaaac
     4681 ttgtttgtcc tctttagaag ccatagctgc tttttcagca agactaccat ttccaaccat
     4741 tgaaccttct acaactgctg gaaaattctt agctactgct tcaggaatat ttttttcatt
     4801 caatttcaat tgtgatgttg gaacattgtt atggctaaaa tcaagatttg taactgtttt
     4861 gttaggtgtt ttaatatcta aagatttaag tttatttcta gaaacactta aggtctcaag
     4921 tgaagtataa ttattaacac cttcaagtga tgttaactga ttattagtca cattcaagaa
     4981 ttttaacgaa ccttgtttat ttttcaatac tagtgactta atttggttcc cttcagcttc
     5041 tactttaaca atttcatcgc tctcttcaat accatctaat gatgataatt tcgcattatt
     5101 aattgttaaa ttagatacct taggattttg ctttaagaaa cttagattgc ttgtattcgt
     5161 ctcatttaaa agaagcgtct caagactagt tgtttttaga gtcgataagt ctgcggattt
     5221 attgtttgat aaatctagaa ctttcaaatc ttttttacca gaaagtgcac ttaaattctt
     5281 cacattatta tgatcaaggt gcaattcttg gagttttgtc aagtttgata acggtgtcaa
     5341 gtcagagata ttgttatgac tcaaaactaa gaattgaagg tttggtaatt cagccaatgg
     5401 ttttagcgaa gtaatgccat tattagctgc agcaataaga cttaattgtt tatattttgt
     5461 aaggaaagat aaatccttaa taccgttttg tgaaatatcc aatccttcta atagtggcat
     5521 cttatcaagg aaagaataat ctgtaatacc agtatttgtc atccaaagct gtttaagttt
     5581 tttgaattgc aaaactggcg tcatatcatt aatcggagta aacccaattc ctagaacttc
     5641 aatattaggc atcaatgata gaccaaaacg ctggaatgga ttggttcttt gtccaatgtt
     5701 aatcttagta acagttgcaa gccactgacg cattttttca ggattagttt catttgatgg
     5761 gaatggtgtt ggagcatctg catgcagaat atcctgaata atctcatcat caaaaccaat
     5821 ttgttttaga gttgccatac caactttatc ttttgcatga gagtgatcgt ggtgtccatc
     5881 tgggataggc ttcccaattt cgatatcctt acttgctata gtgtgtgagt ggtcatcatg
     5941 tggatactta aagatgatac ttccatcctc tgacgttaat acttcaattt gattaggatt
     6001 taaaccaagc ttctctgcta aataaattaa tttcttttga ttttcatcag ataatttatt
     6061 tgatttagtg tgctcgtgtt cttcatgctt atggtcggac tcatcatggt gatgaccttt
     6121 gtgatgatct tcgtcatcat gatgtggatt tggaattggc ttacttagat caatatcttt
     6181 ggccataata acatgtgtat gatcatcatg tgggtattca aatccaattt caccatttag
     6241 tgttttaagg actctgatac gtgaaatatc taaatgtaat tcacgagcaa ggtaagctag
     6301 cttatctttg atgtccttag gtaaagtatt ttcatttctt ggctttttac ttgatggtga
     6361 ctgtttttta gaatcagctt tcttttgtgg ttggtaacgg tcagcaattg atccccaccc
     6421 cccttttctt aaatcagcaa aactaataaa atgcagatga ttatgatgtt ctactaaaat
     6481 acctgttggg gtagtgcctt ttattcctcg tccattaaat aagaaaccat cacttgttgg
     6541 gtagtgaagc cctgcaattt tagaactatt aaccctgtaa tggctattac tttgatttcc
     6601 taaacgtgaa acagctctac ttgcttgtac ttgcttttgc tgagataaac ttgcttttgg
     6661 aatatagtgg aaatggtcac catgtctgac cacgtagcca ttagcatctt ctgaaacaat
     6721 atctgcggga ttaaaaacat agccatcatt taatgtatta acagctaccg cccctgcttt
     6781 aaagtttctt accgcttgat ttttattagt ttttgcatta gcattaggga ttaaatagct
     6841 aaattttgaa ccttttaaat cagagtaaaa aataaaatga gaatgatttc catgtgctac
     6901 aacgattcca gaatctgtat gagataaaat cttcgaatct ttagtcagta aaaagccatc
     6961 atcagttgga aagtcaaccc ctgcaacacc cttgcgtttt ttatgacttt ccttaacaga
     7021 gactttcttt ttattagctt ttttcttagc aacctgcttt gttttaatat gataagaatt
     7081 tttgtgttct tgagtatgac atgccgtaag agcaaaatgg catgacagga caagaccagc
     7141 tagagcaata attttttgac ctttcatgag tcctccataa atgttttttc gttttagtta
     7201 actagttaat tatatcagat gtttttcttt ttgcaatact atttgccatc tttttattat
     7261 ttatttgcta acaaaaactc atttatagct catttatatt taaataaaga tattataaag
     7321 ccatattatt tgataattgc agacaaagaa aaagtaactt aagcacgaag ctaagttact
     7381 ttaaaataga taaatgatat catctttctg aattattttt tgtcatcatc gtccattgat
     7441 aaaacactta agaaagcttc ctgaggaact tcgacggaac caattgcttt catacgcttt
     7501 ttaccagctt tttgtttttc aagcaactta cgtttacgtg aaacgtcacc accataacat
     7561 tttgctaaga catttttgcg tagtgctttt atatcagaac gagccacaat tttttgtcca
     7621 atagctgctt ggataggaac ttcaaactgc tgacgaggaa tgattttttt caacttatca
     7681 acgatcaatt taccacgttc ataggcaaat tctttgtgaa caataaagct taaagcatcc
     7741 actttatcac catttaaaag aatatccatt ttaactaact gtgagcgacg atattcggaa
     7801 atttcataat caaaactagc atagccacgt gttgatgatt taagcttatc aaagaagtca
     7861 aacacaattt cagctaaagg aatttggtaa ataacattaa cacgattatc atcaatataa
     7921 tccattgtta caaagtcacc acgtttacgt tgtgctaact ccattacagc accgacaaat
     7981 tcttgaggaa ccataatttg cgccttaaca tatggttctt caatgctgtc aacacgagta
     8041 ggatcaggaa attcagatgg attcgaaact tcaagcattt caccatcagt tgtattaaca
     8101 tgataaacta ctgatggtgc agtcataata agatcaatat tgaactctcg ttccaagcgc
     8161 tcttggataa cgtccatatg taaaagtcct aagaaaccac aacggaaacc aaaccctaat
     8221 gcttgtgatg tttccggttc aaactgtaga ctagcatcat tcaattgtaa tttttcaagg
     8281 gcttcacgca gatcgttata cttatttgat tcaattggat aaagaccggc aaaaaccatt
     8341 gggttcattt gcttatagcc atgcaagggg tctatagctg ggttattagc aagggtaatg
     8401 gtatcaccaa cacgtgtatc agcaactgtt ttaattgagg cagcaatata ccctacatct
     8461 ccagttgcta aaaaatcacg tccaactgct ttgggagtga aaataccaac ctcagtaacg
     8521 tcgaaggttt taccattaga catcatttga atcttatctc ctggcttaac cataccattt
     8581 acaatcctta cttgcagaat tactccacga taagcatcgt aaacagaatc aaaaattaga
     8641 gcttgaagag gagcatctac ttccccagtc ggagcaggaa ctttttcaac gatctgctct
     8701 aaaatttctt caataccaat ccctgcctta gctgaggcaa gtacagcttc tgaagcatct
     8761 agaccaataa catcctctac ttctgcacgt acacgttctg gatctgctgc tggtaaatca
     8821 attttattaa taactggtag tatctctaaa tcattgtcta gagccaaata aacatttgcc
     8881 agagtttgag cttcaattcc ttgggctgca tcaacaacca gtatagcacc ctcacaagct
     8941 gccaatgaac gtgatacttc atatgtaaag tcaacatgcc ctggcgtgtc aatcaagtga
     9001 aaaatgtaag tttcaccatc tttagcagta taattaagct caatagcatt taactttata
     9061 gtaaacccac gctcacgttc taggtccata gagtccaaca gttgcgcctg catttcacga
     9121 ctagaaaccg tttctgtttt ctctaaaata cggtctgcta aggttgattt tccatggtca
     9181 atatgagcga taatcgaaaa gtttctaatc ttttcttgac gtttcttcaa atcttcaata
     9241 ttcattctat ttcctataca atttgatatg tttaacttct aaaatattaa aacagtccta
     9301 tagactctag aatattatat caaaaagaag accgatgtaa aagagaatta acacttgaac
     9361 atcaatcttc attaagtcta ttagttattg aaccaaatgg ctatctcgcg agctgcagat
     9421 tcaggtgagt ctgacccatg aacaatatta catgtcgctt ggtttggaga gggagcttgt
     9481 gcaaaatctc ctcgaatagt tcctggaagg gcatctttgg ggttagtact ccccatcatg
     9541 gtgcgccaag ttgaaatgac ctcttctccc gaaataactc caatgattac tggaccacta
     9601 gtcatataat ctactaatgt tggaaagaat ggtctatccg tcagttctgc ataatgtctt
     9661 ttcaaaatat ctgcatctgc atgtctaacc tctaagcgat caatagagaa gcctcgacgc
     9721 tcaatacgac taataacttc tccaataaaa ccacgtttta caccatcagg ttttatcata
     9781 aaaaaggttt gttccataat attagcacct catttctttc tccactatta taacatgaaa
     9841 atatgttttc tgtaacttcc aactgtattt caaaaaagga gtgtcggaca ctccttaact
     9901 atattaatag gtgagatcca tcatagctgt tagttgatta tggataggtt ctccactgct
     9961 ttccttgtaa gcttgcaagt attagtgaca aagaaacacc agcaatcact aaaagaacca
    10021 acgctgtcaa actaataatt aagtaaagcg tagaacaatc tagcccttta accataaggt
    10081 tggaattata gtctttcgta tctcttctat cgacacattc tctttgaatc cgaaaaataa
    10141 ttcgaatttt ctactaatag acacacctct aaatgacgtt catatttttt agtcgccaat
    10201 atataagttt cccaatcaaa ctgataaact ggggtgattt cgttagcttg gccaactaag
    10261 ttctgaatta cgtcttgaat taatctaatt accgccttcc cccacattct ctgaaaattt
    10321 atttcagtat aacaaaaaac tagaacacca caacggtgcc ctagcataat aaccgcacag
    10381 ctctaaaaca catatcagga atataaaaat cgttcaaatg ttttggaacc attcgaaaca
    10441 gcacagctct aaaactgcct tttaattcag ctcccacttt ttatcgtttt ggaaccattc
    10501 gaaacagcac agctctaaaa cttgtatatg acccgttacc gctatcaaat agttttggaa
    10561 ccattcgaaa cagcacagct ctaaaacttg tatatgaccc gttaccgcta tcaaatagtt
    10621 ttggaaccat tcgaaacagc acagctctaa aaccatgatg ttatggtata cggacaatac
    10681 ttagttttgg aaccattcga aacagcacag ctctaaaact ttattttttc tcagttcctt
    10741 gattttagag ttttggaacc attcgaaaca gcacagctct aaaactaaat actttagaag
    10801 aaagagttgg tttttgtttt ggaaccattc gaaacagcac agctctaaaa ctcagaaagg
    10861 ttgatagaat aatgtttcca agttttggaa ccattcgaaa cagcacagct ctaaaacagt
    10921 gtattcttgg ttatctttag ctatattgtt ttggaaccat tcgaaacagc acagctctaa
    10981 aactagaaat ccttaggcgt tcctcttaaa gtagttttgg aaccattcga aacagcacag
    11041 ctctaaaact tttccaatca cggttcatgt tccaacggtg ttttggaacc attcgaaaca
    11101 gcacagctct aaaacttgac tgattttgcc caattgctcg tgcaagtttt ggaaccattc
    11161 gaaacagcac agctctaaaa ctttgcttct aaaaaaaata cagatgaaga tgttttggaa
    11221 ccattcgaaa cagcacagct ctaaaacctc gtagaaaatt ttcttttgag ctttcgtaat
    11281 cgcgccattc gtctcagcag gacttcagtt tcgatgattc cttgttatta ctgtgctttt
    11341 actaatatta taccatattt tcgcctatca agaaataatc cttatcaata acatattgcg
    11401 gtaaatcata gagtcttcta ggttctagaa agagtactga cttttgcatt aaattgatgt
    11461 attcacataa ttttataact tcatctttgg taagataagc tccgctatta acaaaaacca
    11521 agagattctt tttcgttaaa taatggtaaa cttgtataat ttcaaaacat ttttcaaaga
    11581 tagtgtcgct ctgtgtctca attttgactc ctagtgcctt aatgagttct aaaatcgtga
    11641 tttcatcgta ttctaaatca agctcattct ctagacactc aaatgcgata agttctgtaa
    11701 tagtagctgc taatttttct accattgatt tcacttctgg cttttcgttg aattgatttt
    11761 ctaagtcacc atgtatcaac tttagaatag gtgctgagtt gacatcgtat cctaagatat
    11821 ctgtaaccat cattaactct gttgccttca gacttttctg cttatcatca aataatttca
    11881 aatgttcatc tacgtcatat tgataaaaat gtttcaccaa tgaagaataa actgaaacat
    11941 cttctatcgt taaaatcgta gcattactta acactaatgg ttcatctaaa attggaaaat
    12001 taatcttgat cataagaatc tcctaggaaa accaagcgac tatcaaagtt ggcaacactt
    12061 gtgttgcgtt ctccatggag gtaaaccatt ctcgcaaatt gtttttccgt aactgttaag
    12121 agtgtgatat tacccttctt aggattattc actttaagcc gacctatcat agcattatta
    12181 gctgtattat tgagtaataa tttactataa acagagaact gatgcataat aaaaccttcg
    12241 ctcaaaagaa actttctaaa cttacgatac gccttacgtt cttctactgt ttcagtaggc
    12301 atatcaaaca ttaaaatcat tctcatatac cgataactca tatcctaaac tccggaatcc
    12361 cattcccatc actatttagc gacttaataa cttttttggt gtaatcgctg acaatatttg
    12421 aaagatacat ttctttaccg tcgtaactgt atgtctctga aaacatagaa aagagttctc
    12481 ttttcatttt gacaaaatca ctctgcctat tttcataaat aatcctatca acgattgggc
    12541 gaaatggttc cataatatcg cttgctaggt tgaactgatt aaattgatta gcatgcttta
    12601 acccaaattg tgtcatgcaa ccacacttaa cgacttcacg cgcaaacata ctcaaaagca
    12661 atgaatatcc gtagtctaaa ccagcattta ttggattatc atgttctctt gaaaaatcat
    12721 ttccaaaaag tgtattgaaa taaatcctag ctgcatgccc ttcacgattg gaagtatcaa
    12781 aagattctaa atcatgatag agattcataa tagactggga tttttcaaag aaagaacact
    12841 caccaagata aaaagactga ttcaaaattt tttgtgcaat gattgacgtc caaacatccg
    12901 ctttgacatc ttcaatccat gacatctgcc tagataattg taaactcgaa tcatatctgg
    12961 catagtacgg cattaacata gctgttggca agcgtttgtc atcacaaaat ataactaaaa
    13021 tattttcatc aaccaaacgt ttaatcagca tggtcgacaa aacgatatct gttgtttcca
    13081 tgattagaat gtcaatctct gatagatgaa tcatttccgt ctgataagaa tctttaaaaa
    13141 ccagatgatt atttttataa gagagcttag aatgtgtatt tacaacaacg gttcgccaac
    13201 ctgccatatc aatcttctcc taacttaccc aaatcaatac gtgtttcata aagtccagta
    13261 atagattgat gaattagggt agaattaagt acttctttag ttgatgtata gcgttttcta
    13321 tcaactattt tatcaaaaaa tttaaaagct gctggagctc ctagactggt aaaagtaaat
    13381 agattgataa tattattagc aagttcatct actgatatat tttccttatt atcttggtaa
    13441 agcttattga ttttctctaa attagcatct gctagaataa ctcgatttga aaaatcgttg
    13501 attatttgaa gaatatcgtc gaaataagaa acatgttgaa ctacaaattc ctgtttttgt
    13561 tcaatttctt ctggttttcc ttttagctca ttgtaacgac ttgcaaggta taagaacttc
    13621 atatattgtg ttggtaaggc tagctcgtta cctttttgta attcaccagc actagcaagt
    13681 aatcgacgac gcccattttc taattcgaac agactatact tcggtaaaat cattaattta
    13741 tcgtccctaa tatttaaata accttttgat tcaaggaaag ctgatggatt tttctcaaat
    13801 ctgaatctct ccatgatggt aattcctaaa agttccgtaa ctgtttttag tttttgtgct
    13861 ttaccttttt tgatatcagc tacaactaaa acagagtaag ctactatcgg actatcaaaa
    13921 cctccatatt tcttaggatc taaataaata tccttcgttt ttcttggaat caacttatct
    13981 gagttaccat gcgccaagat tgattcctta gagaaaccac ctgtctgaat ctctgtcttc
    14041 ttcacgatat tgacctgagg gtatgacaag acttttctaa ctgtcgcaaa gtgtttcttc
    14101 ttatcccaaa caatttcacc cgtatcatta ttaacttcaa tatcatcttt tacaacaacg
    14161 gttccatccg ccaaagttac cttagtttta aagaagttca taatatttga atagaaaaat
    14221 agcttttctg tagcggattt acgcgttttg taactgttat attttggata gtcaccgtag
    14281 acaaattctg gctctaactg aggatatttg gttagaatag ctttagcaac tactgcatta
    14341 agataggcat catgtgcatg gtgataatca ttaacttcac gaattttata gaatccaaat
    14401 tcttttcgga aatttgaaac caaatttgac ttcaaggtta caattttaac tttgcgaatc
    14461 cttctacctt tactatcaag ctcattatta aagcgttcat ccaagatacg ggcaacatgc
    14521 ttggtaattt gtcgtgtctc aaccaactga cgttggataa atcttgcctt atcatcggaa
    14581 gttaggcctc cgcgctctgc cttagtcaaa ttatcatact tacgctgact cattaactta
    14641 gcatcaagta attttttcca gaatgctttg cgtgctttta caatatcaat gctaggaaca
    14701 ttatctgatt ttccacggtt tttagcagat gataccaaaa cacgattatc aatagaatca
    14761 tcttttatga aagcttgagg aacaatgtga tcaatatcat attgacttaa attgtcaata
    14821 tctagtgctt cccctgtgta catatctctt ccattttgta agtaataaag gaaaagtctt
    14881 tcattttgca acgcttgatt atccgtagga tattctttca aaatattgcc attcaagtca
    14941 ctagctagat tcttaacgcc ttcctcaaga agtttatagc gttgtcgagt gttacgacga
    15001 ccttgatttg ttgtttgatt ctcacgcgcc atctcaacca caacttgttc aggttcgtat
    15061 cccatgactt taacaagctc atcaacaatt ttcaaacttt gtagaatccc ctttttaata
    15121 gcagggctac ctgcaagctc acctacaact tcttttagat tatctgaatg actaccagcc
    15181 tgtgccttgt caataattgg tttaaatgat agaccatcat catgtatcaa ctgcataaag
    15241 ttgcgatttg ctcttccatc agaaatcaga tagtccaaga ttgttttttg actctcttta
    15301 tctctaatgc cattaattaa cttagcagac aatcgtcccc agccagtata gtgacggcga
    15361 tagagttttt ttagttgtga ctctgtaaaa agatctttat agttttcaag acgcttctta
    15421 atcatttctc tatcttcaaa taaggttaga gtttggacga tatcttccaa aatagactca
    15481 ttatctggat tatcaagaaa atctttgtct agtatttttt tgagatcgtg gtaagttccc
    15541 aatgaggcgt tgaaagcttt attttcttta tctagaccaa taacatctac tatcctaaac
    15601 tcctcatatt ctttagccag aaaatctagc aacttcttct tggatacctt acgatgttcc
    15661 ttgaatactc catcaaagat ttcttgttta atattgctat caaaaaaata agtctcacct
    15721 tgctcatttt tatatctaac cttagtcaac tcattataga ccgtaaattt ttcataaata
    15781 agactatgtt ttggtaggac tttttcttca ggaagataga ggtcattgtt ggtcatacga
    15841 tggataaaag cttccgcaga tgcttcctta tcaaccaatt cttcaaaatt ccaaggtcga
    15901 atactgtcat ctgttttgcg agtcatccat gcaaaatcac tcttctcacg tgctagtggc
    15961 ccgacataat aaggaattct aaaggtaagg attttttcaa tcctatctag attctctttc
    16021 aagaatggat agtattctga ttgacgtcga ataatagcct tcaattctgt caaatggact
    16081 tggtgtggaa ttgagccatt atcaaaggtc ctttgttttc tcaaaaaatc ttcattcttg
    16141 attttttcaa gaaaatactc gctaccttct tgcttggtca acaattttga caagtattta
    16201 taaaatgctc cttgattagt tttgccttca atataaccag cgtagccatc ttttgatgaa
    16261 tcagtaaata tttcttgata tttctcaggc aatgaagctt ttacgaattg ttttaactgt
    16321 ttcaagtcct ctctatgttc atcgtaacgc tgaatcatag aggcagaaag tggcgccttg
    16381 gtactgagat cagttactgt aagaatgcca gataaaagaa cactatcata tagctttttc
    16441 gctactgaga ataagtcagc aaattcatca ccaatctgcc caagaagatt ttctaaatct
    16501 tcatcgtagc tatccttagc aaattgaagc ggcgttttat cttccaaatt gaaatgtttc
    16561 ttgaagtcag cttgatttcc gacaatcaat ttcaaaaatt ctgcaaaaat gccagtagat
    16621 ttttggttag ggtactgcgc taaaatgcga tctttcttcg cagacttgct aatcttatct
    16681 gttagaattc cctctacatc tatgttttga gataacaaat ggttattttc gaaggtagta
    16741 tcaaagattt ctaaaaaagc ttgatattgt ctttgaatat cagtattcct cacatcaaag
    16801 ctatcatcct caattaggaa atgtcctctg aatttaatga tatgagctag agccagataa
    16861 acaagacgaa ggtctgcttt ttctttcttg tcagctaatt cttttctcaa gtgataaatc
    16921 gttgggaact tttcatgata atctttctcc tcctgcattg ttgcaaagat aggatacttg
    16981 ctccctctct tgtcgtcctc aactagaaaa gaatcctcta atcgatgaaa gaaactatca
    17041 tcaactttac tcatttcctc tgcaaaaatt tcttgtaaat atagaatacg atttctacga
    17101 cgtgtataac gacggcgagc agttcgcttc aagcgtctat ctgaagcagt atttccacca
    17161 tcaaaaagca gagcacctat aagattcttc tttatgtatt ctttatctgt attccctaag
    17221 accctcatct tcttagcagg taccttataa tcatctgtaa ccacagaata tccgacggaa
    17281 ttagttccga tgtcgagacc tattgaatat ggtttgttca taatttatct ccttttattt
    17341 aaaatatcac tttaaaaacg caaactttaa agttttgcta atttttactt gattattaca
    17401 ttaaaataac atataataat tttg
//
LOCUS       DATROD010000028        17325 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.028, whole genome shotgun sequence.
ACCESSION   DATROD010000028 DATROD010000000
VERSION     DATROD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17325
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.028"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(34..744)
                     /locus_tag="VBC74_000929"
     CDS             complement(34..744)
                     /locus_tag="VBC74_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6941892.1"
                     /translation="MNIKRHSIFLWLTTSILAILMAVGMTIYFNTYQASPLARRIAKT
                     ATNHKNYLLFRSKGKVKANIIFYQGALVEEEAYSQLARDLADKGYNTYILKTPLNLPV
                     LSPYKAKTIINQNHLTNVYLAGHSLGGVVASQNAKVAPVRGLILLASYPSSKSDLSHK
                     KLRVLSITASNDHILNWEKYEEAKKRLPNSSTFRTIVGGNHSRFGNYGHQKGDGKATL
                     SHKSSEKQLATFISNFIH"
     gene            complement(741..1439)
                     /locus_tag="VBC74_000930"
     CDS             complement(741..1439)
                     /locus_tag="VBC74_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001907086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO6941893.1"
                     /translation="MPFIFRHSFFNKVLIFWYTIIMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDTFDNPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIINVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPSHYSVHPGHGMN
                     TTIGHEKNFNPFFQ"
     gene            1607..2371
                     /locus_tag="VBC74_000931"
     CDS             1607..2371
                     /locus_tag="VBC74_000931"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEO6941894.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGVLDYNEETAQAAVD
                     KLSPEDAVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGIAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIVHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLAGDDSNYITGQTIVVDGGMVFH"
     gene            2479..4986
                     /locus_tag="VBC74_000932"
     CDS             2479..4986
                     /locus_tag="VBC74_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEO6941895.1"
                     /translation="MFCFIDIACYNRLTMTQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLVKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEQLFLEGFELRTPRIDTVELSQVFYPCLEKYSLGALAESLNIEL
                     TDAHTAIADARATAQLFIKLKAKISSLPKEVLETILTFADNLLFESYLLIEEAYQEAD
                     FVNPKEYYFWQGLVLKKEKAVGKPKKLSSDFQVNMALLGMDARPKQIVFADLVKAHFN
                     DQTTTFLEAQSGLGKTYGYLLPLLDQSQKQQIIVSVPTKILQDQIMAKEIKHIQELFH
                     IPCHSIKGPRNYLKLDAFYKSLQVQDRNRLINRFKMQLLVWLTETTTGDLDEIKQKQR
                     LESYFDQLKHDGEVTQSSLFYDLDFWKRSYDKVAQSQLVIINHAYFLERVQDDKDFAK
                     GKVLVFDEAQKLVLGLENFSRGQLDISHQLQVIQKIIDSPIPLLQKRLLESISYELSH
                     AAELFYRHNSFEFSETWLKRLKNSINALEVVGLDDLQTFFTATYTNYWFETDKVNEKR
                     LTILRGAREDFLKFSEFLPPTKKTYMISATLQISPKVYLSDLLGGFSSISTEKIAHEK
                     NANQKVWIDTSMPNILDLSPEQYAYEIAKRLQDIMTLKQPTLVLLTSKQTMFMVSDYL
                     DKWEIKHLTQDKNGLAYNVKKRFDRGESNLLLGTGSFWEGVDFVHRDRLIEVITRLPF
                     DNPKDYFIQKLSQSLTKEGKNFFYDYSLPMTVLKLKQALGRTTRREEQKSAVIILDSR
                     LVIKSYGQTIMHSLGRDFEISKEKINKVLTEMAKFLI"
     gene            5072..6265
                     /locus_tag="VBC74_000933"
     CDS             5072..6265
                     /locus_tag="VBC74_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6941896.1"
                     /translation="MTYLSERVLNMEESVTLAAGAKARELRVQGRDILSLTLGEPDFA
                     TPKNIQQAAIEAITDGRASFYTPSSGLPELKSAINAYFERFYGYSLKPNQVVVGTGAK
                     FILYTFFMTVLNPGDEVIIPTPYWVSYADQIKMAEGKPVFVTAKEVNHFKVTVEQLEA
                     VRTDKTKVILLNSPSNPTGMIYKAEELEAIGNWAVEHDILILADDIYGRLVYNGNIFT
                     PISSLSESIRNQTIVINGVSKTYAMTGWRVGFAVGNHDIIAAMSKVVSQTTSNLTAVS
                     QYATIEALNGSQESFEKMRLAFEERLNIIYPLLCQVPGFEVVKPQGAFYLFPNVTKAM
                     EMKGYTDVTAFTDAILEEVGLALVTGAGFGAPENVRLSYATDLGTLKEAVRRLHVFMG
                     SNEIN"
     gene            6286..7632
                     /gene="asnS"
                     /locus_tag="VBC74_000934"
     CDS             6286..7632
                     /gene="asnS"
                     /locus_tag="VBC74_000934"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEO6941897.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEVIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYSTYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYIAEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIKP"
     gene            complement(7672..8229)
                     /locus_tag="VBC74_000935"
     CDS             complement(7672..8229)
                     /locus_tag="VBC74_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6941898.1"
                     /translation="MTKSPARLISFISIAIAINLIGANLALFLRLPIYLDTIGTLLIA
                     VILGPWYAASTAFLSALINWMTTDIFSLYYSPVAIVVAIITGILIKRNCKPSSLLWKS
                     LIISLPGTIIASVITVILFKGITSSGSSIIAQFLHGIGLDMTSSLILVQVGTDYIDRL
                     ISLILVFSTITLLKKHSPNLINQRF"
     gene            complement(8226..9209)
                     /locus_tag="VBC74_000936"
     CDS             complement(8226..9209)
                     /locus_tag="VBC74_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEO6941899.1"
                     /translation="MNKEKIIIDCDPGIDDTLALMYAIQHPKLEVVAITITAGNSPVE
                     LGLKNTFVTLELLNRRDISVYVGNNLPLQREFVSAQDTHGMDGLGENNFTLAQPIIFQ
                     EESADCFLANYFEHKNDTSIIALGPLTNIARALQTNPKLGKHCKRFVSMGGNFKSHGN
                     CSPVAEYNYWYDPHAAQYVFENLDKKIEMVGLDITRHIVLTPNHLSYMERINPDVSTF
                     IQKITKFYFDFHWQYEHIIGCVINDPLAIAYFVNENIATGFDSYTDVACHGIAMGQTI
                     VDQYHFYKKDANSKILTSVNTNLFWNDFMTVLLHAQNSVIIEDLEMLGLNK"
     regulatory      complement(9353..9397)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            complement(9455..9868)
                     /locus_tag="VBC74_000937"
     CDS             complement(9455..9868)
                     /locus_tag="VBC74_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEO6941900.1"
                     /translation="MLYEVTSSNTQGVDGKVYLSNGKIVETNHPLNHLPGFNPEELIA
                     LAWSTCLNATIKAILEQKGFKDLKSRVDVNCQLMKEKQVGKGFYFQVNAVASIEKLSL
                     SDSKLIVNKAHSRCPISKLISNAKTINLDTVKWSV"
     gene            10022..10912
                     /gene="rapZ"
                     /locus_tag="VBC74_000938"
     CDS             10022..10912
                     /gene="rapZ"
                     /locus_tag="VBC74_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEO6941901.1"
                     /translation="MSDEQIKLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPTLVPK
                     FLELAAQSGDTSKIAMVVDMRSRLFFREINSILDSLEINDNINFKILFLDATDTELVS
                     RYKETRRSHPLAADGRVLDGISLERELLAPLKSMSQNVVDTSELTPRQLRKVISEEFS
                     NQDSQSSFRIEVMSFGFKYGIPLDADLVFDVRFLPNPYYKPELRDKTGLDTEVYDYVM
                     SFDESDDFYDHLLALIKPILPGYQKEGKSVLTVAIGCTGGQHRSTAFAHRLSEDLKAD
                     WTVNESHRDKNKRKETVNRS"
     gene            10909..11883
                     /locus_tag="VBC74_000939"
     CDS             10909..11883
                     /locus_tag="VBC74_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEO6941902.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLRLEDVEITAVVTVADDGGSSGE
                     LRSVMQLTPPGDLRNVLVALSDMPKFYEQIFQYRFAEGDGDFAGHPLGNLIIAGVAEM
                     QGSTYNAMQSLTQFFHTTGKIYPSSEHPLTLHAVFKDGHEVVGESQIADYKGMIDHVY
                     VTNTYNEETPTASRKVVDAILESDMIVLGPGSLFTSILPNLVIPEIKQALLETRAEVA
                     YVCNIMTQRGETEHFTDADHVEVLKRHLGQDAIDTVLVNIEKVPESYMENNHFDEYLV
                     QVEHDFSGLRKHARRVISSNFLKLEKGGAFHHGDFVVEELMNLVGTHL"
     gene            11880..12791
                     /gene="whiA"
                     /locus_tag="VBC74_000940"
     CDS             11880..12791
                     /gene="whiA"
                     /locus_tag="VBC74_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000011317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEO6941903.1"
                     /translation="MSFTVKVKEELLGHKSENKMELSAIIKMSGSLGLANHGLNLSIT
                     TENAKIARHIYSMLEEHYHLQPEIKYHQKTNLRKNRVYTVFIEEKVDVILADLKLADA
                     FFGIETGIEHSILDNDENGRAYLRGAFLSTGTVREPDSGKYQLEIFSVYLDHAQDLAN
                     LMKKFMLDAKVIEHKHGAVTYLQKAEDIMDFLIVIDAMEARDAFEEIKMIRETRNDIN
                     RANNVETANIARTITASMKTINNIIKIMDTIGFDALPSDLRQVAQVRVAHPDYSIQQI
                     ADSLETPLSKSGVNHRLRKINKIADEL"
     gene            12806..14203
                     /locus_tag="VBC74_000941"
     CDS             12806..14203
                     /locus_tag="VBC74_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEO6941904.1"
                     /translation="MACTTILVGKKASYDGSTMIARTEDSVNGDFTPKKLKVMTSKDQ
                     PRHYKSVLSNFEVDLPDNPLPYTSVPDALGKDGIWGEAGINSKNVAMSATETITTNSR
                     VLGADPLVSDGIGEEDILTLVLPYIQSAREGVERLGAILEKYGTYESNGIAFSDTEEI
                     WWLETIGGHHWIARRVPDDVYVTNPNQLGIDHFEFNNCDDYMCSSDLKEFIEQYHLDL
                     TYSNEHFNPRYAFGSQRDKDRHYNTPRSWAMQRFLNPEIEQDPRSLFIPWCQKPYRKI
                     TVEDIKYVLSDHYQDSVYDPYGPEGDAVSRRAFRSVGINRTSQTSILQLRPNKSLETT
                     GVQWLSYGSMPFATMVPLFTQVETVPDYFSNTTKDASTDNFYWTNRLIAALADPHFYQ
                     HEADIESYIERTMAQGHAHINGIDREVAENKEIDFQQKNQEMSDYIQKESQELLNRIL
                     FDASNLMTNRFSMGD"
     gene            14345..15865
                     /locus_tag="VBC74_000942"
     CDS             14345..15865
                     /locus_tag="VBC74_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEO6941905.1"
                     /translation="MRKKFLLLISFVAMFAAWQLVQVKQVWADSKLKVVTTFYPVYEF
                     TKNVVGDKADVSMLIKAGTEPHDFEPSTKNIAAIQDSNAFVYMDDNMETWAPKVAKSV
                     KSKKVTTIKGTGDMLLTKGVEEEGEEHEGHGHEGHHHELDPHVWLSPERAISVVENIR
                     NKFVKAYPKDAASFNKNADAYIAKLKELDKEYKNGLSNAKQKSFVTQHAAFGYMALDY
                     GLNQVPIAGLTPDAEPSSKRLGELAKYIKKYNINYIYFEENASNKVAKTLADEVGVKT
                     AVLSPLEGLSKKEMAAGEDYFSVMRRNLKVLKKTTDVAGKEVAPEEDKTKTVETGYFK
                     TKDVKDRKLTDYSGNWQSVYPLLQDGTLDPVWDYKAKSKKDMTAAEYKKYYTAGYKTD
                     VESIKIDGKKHQMTFVRNGKSQTFTYKYAGYKILTYKKGNRGVRYLFEAKEKDAGQFK
                     YIQFSDHGIKPNKAEHFHIFWGSESQEKLFEEMENWPTYFPAKMSGREVAQDLMSH"
     gene            complement(15978..16238)
                     /locus_tag="VBC74_000943"
     CDS             complement(15978..16238)
                     /locus_tag="VBC74_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEO6941906.1"
                     /translation="MKKDIHPDYRPVVFLDTTTGYKFLSGSTKSTKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAQ"
     gene            complement(16347..17282)
                     /locus_tag="VBC74_000944"
     CDS             complement(16347..17282)
                     /locus_tag="VBC74_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEO6941907.1"
                     /translation="MIIFQQILDKIKEYDTIIIHRHMRPDPDALGSQIGLRDIIRHNF
                     PKKKVLATGFDEPTLAWIAKMDQVTDQDYQGALVIVTDTANTPRIDDERYKKGDFLIK
                     IDHHPNDEVYGDLSYVDTNASSASEIVTDFALSCDLLLSTSAARVLYNGIVGDTGRFL
                     YPATTSKTLKIASKLREFDFDFSAMARQMDSFPFKIAKLQGFIFEQLEIDKNGAACVT
                     LTQEDLKRFDVTDAETAAIVGIPGKIDIVESWAIFVEQSDGHYRVRLRSKSHIINEIA
                     KRHNGGGHPLASGANSYSLEENQAIYQEIQEVLSL"
BASE COUNT         5637 a         2780 c         3461 g         5447 t
ORIGIN      
        1 tttcatctct ttttattaat tgtagatatc atgctagtgg ataaaatttg aaataaacgt
       61 agccaattgt ttttcagatg atttgtgaga aagggtagct ttaccatccc ctttttgatg
      121 tccgtagtta ccaaatcggc tatggtttcc cccgacaata gttcgaaaag tacttgaatt
      181 tgggaggcgt tttttagctt cctcatattt ttcccagttt aaaatatgat cgttacttgc
      241 tgtaatggag agtactctta actttttatg agataggtcg gatttacttg aagggtagga
      301 agctaataat atgagccctc taacaggggc tactttggca ttttggctag caacaactcc
      361 tcctaaagaa tgtccagcaa ggtaaacatt agttaagtgg ttctgattaa taattgtttt
      421 agctttataa ggtgataaaa ctggcaaatt taaaggtgtc ttcaaaatat aggtattata
      481 ccctttatca gctaaatctc tagcaagttg agaataagct tcttcttcca caagagcccc
      541 ttgatagaaa ataatatttg cttttacctt acccttgctt ctaaaaagaa ggtagttctt
      601 atggtttgta gcggttttag caattctcct agctaatggg gaggcttggt aagtattgaa
      661 gtaaatggtc atcccgacgg ccattaaaat agcaaggata cttgttgtta accaaagaaa
      721 aatagagtga cgttttatgt tcattggaag aaaggattaa agtttttttc gtggccaata
      781 gttgtattca tgccatggcc tgggtgaaca ctataatgac taggtaaggt gaatagttct
      841 tggcgaatac cagtaatgag gtcctcaaag ttactggtag gtaagtcagt acgaccgata
      901 gtttcacgaa atagagcatc gccagtaacg actaattcat ctgagtggaa aacaaatgag
      961 actcctcccc aagaatgacc aggtgttggt aaaactgtga attcaaaacc atttagttga
     1021 tagggttgct ttaatttaaa aaagttctct gccggtcttg ctataacatt aatgatatca
     1081 tcatgacggc ctaacccaga taaattatca tctggggaag atagccaagc ggcttctttt
     1141 tctgaaacat aaacaggtgg attatcaaaa gtatctctga ccaaatctaa actaaaaata
     1201 tgatcatagt gagtatgagt taagaggata gccactaaag gtttttcaaa ggatttaatt
     1261 ttagcaatga tttcttggcc attgctccct ggatcaatga ggataacagc ttgatcatta
     1321 acgaggtaat aagtattttc accagcaatg tggtttattg ttttatagat tttcataatg
     1381 atagtgtacc aaaaaatcaa gactttgtta aaaaaagagt ggcggaaaat aaatggcaac
     1441 tagtttcaga aaatatggga tctcacagca atttcttgac accgttttca taaaggtgta
     1501 tcataaaagt aatcaataag ccaagcctaa agctgaactt agtttaagaa cactgacttt
     1561 ttgagagcaa cctaattggt tgactaaaga atatgaggtg gaagatatgt caaaagttgc
     1621 tattgttact ggtgctggtc aaggaattgg atttgctatt gcgaaacgtc ttcatgcaga
     1681 cggctttaaa ataggcgttt tagactataa tgaagagact gctcaggctg cggttgataa
     1741 actatcacct gaggatgctg ttgcagttgt agcagatgtt tcaaaacgtg atcaagtctt
     1801 tgatgctttc caaaaagttg tagatacttt tggtgatttg aatgttgttg ttaacaatgc
     1861 aggtattgcc ccaacaaccc ctcttgacac tattactgaa gagcagtttg aaaaggcttt
     1921 tgcaatcaat gtcggaggaa ctatttgggg aagtcaagca gctcaaaaac attttagaga
     1981 attaggtcat ggcggaaaaa ttataaatgc aacttcacaa gcaggatgtg aagggaatcc
     2041 taatttgact gtttatggtg gtacaaaatt tgcagttcgt ggtattacac agactttggc
     2101 aaaggatttg gcatcagaag gaatcacagt caatgcctac gcaccaggta ttgttaaaac
     2161 acctatgatg tttgatattg tccatgaagt tggtaagaat gcaggcaaag atgatgaatg
     2221 gggtatggag cagtttgcaa aagatatcac tttgaaacgc ttgtctgaac cagaagatgt
     2281 tgctaatgca gtaggcttct tagcgggaga tgattcaaat tatatcactg gacaaacaat
     2341 agttgtcgat ggtggtatgg tattccatta aaaatgatta atgctaacac tgtgagtgaa
     2401 actcacagtg ttttttatat ttcttaaaaa attatcttat cttggttatt ggagtgatgg
     2461 catctttgag gtacagtcat gttttgtttt atagatatag cgtgctataa tagacttact
     2521 atgacccaga aaaaacttag aaaatatgca gtagttgact tggaggctac tggagcaggt
     2581 cccaatgctt ctattattca ggttgggatt gttattatcc aaggaaataa aattattgat
     2641 agttatgaaa cagatgttaa tcctcatgaa tctttggatg agcatattgt tcatttgaca
     2701 ggtattacgg ataagcagtt ggttaaagct ccggattttg gtcaagtggc gcatcacatt
     2761 tatcaattga ttgaagattg tatttttgtt gcccacaatg taaagttcga tgcaaattta
     2821 ttggctgagc aactattttt agaaggcttc gagttacgta caccaaggat agatacagta
     2881 gagttatcac aagtgtttta tccctgttta gaaaaatatt ctttaggggc attagcagaa
     2941 agcttaaata ttgaattaac agatgcgcac actgctattg cagatgcaag agctacagca
     3001 cagttattca ttaagttaaa agctaaaatt agtagtttac ctaaagaagt gttggagacc
     3061 attttaacat ttgctgataa tcttttgttc gaatcctatt tattgattga agaagcttat
     3121 caagaagctg atttcgttaa tcctaaagaa tattatttct ggcaaggatt ggtcttaaaa
     3181 aaagaaaaag ctgttggaaa gcctaagaaa ttatctagtg attttcaagt taacatggct
     3241 ttgttaggta tggatgctcg ccctaagcaa attgtttttg ctgacttggt taaagcgcat
     3301 tttaatgatc aaacaacaac gtttttagaa gctcaatcag gacttggtaa aacatatgga
     3361 tatctcttgc ctttattgga tcagtctcaa aaacaacaaa taatagtatc tgttccaaca
     3421 aagattttgc aagatcaaat aatggctaaa gaaataaagc acattcaaga actttttcat
     3481 attccctgtc acagtatcaa aggaccgaga aattacttga agttagatgc gttttataag
     3541 agcctccaag tgcaggatcg aaatcgattg ataaatcgtt ttaaaatgca actgctagtg
     3601 tggttgacgg aaacgactac aggtgatttg gatgaaatta aacaaaaaca acgattagaa
     3661 tcttactttg accagctaaa acacgatgga gaagtgactc aatcatcact tttttatgat
     3721 ctggattttt ggaaacgcag ttatgataaa gttgcacaaa gtcaacttgt tattattaat
     3781 catgcttatt ttcttgagcg tgttcaggat gacaaagatt ttgcaaaggg gaaggtttta
     3841 gtttttgatg aagctcagaa acttgttttg ggactggaaa atttttctcg tgggcaactt
     3901 gatattagtc accaattgca ggttattcaa aaaataattg atagtcctat tccgttatta
     3961 caaaagcgcc ttctggaaag tatttcttat gagttaagtc atgcagcaga acttttttat
     4021 cgtcataatt cttttgaatt ttcagagacc tggttgaaac gtctaaaaaa tagtataaac
     4081 gctttagaag tagtaggatt agatgattta caaacttttt tcacagctac ttatactaac
     4141 tattggtttg aaactgataa agtcaatgag aagcgtttaa ccattttacg tggggcgaga
     4201 gaagattttc ttaaattttc agagttcctc cctcccacaa aaaagaccta tatgatatca
     4261 gctactttac aaatcagtcc caaagtctac ttgtcagatc ttttaggagg attttcaagt
     4321 ataagcacag agaagatagc gcatgagaaa aatgccaatc agaaagtatg gattgatacc
     4381 tctatgccta atatacttga tttatcaccg gaacagtatg cttacgaaat agccaagcgt
     4441 ttacaagata taatgacgtt aaagcagcct accttagtct tattaacttc taaacagaca
     4501 atgtttatgg tatctgatta tttggataag tgggagataa aacatcttac tcaagataaa
     4561 aatggcttag cttataacgt taaaaaacgt tttgatcgtg gagaaagcaa tcttctttta
     4621 ggtacaggat ctttttggga aggtgttgac tttgtacatc gtgatcgtct tattgaagtt
     4681 atcacgcgat tgccttttga taatcccaaa gactatttta tccagaaact atcgcagagt
     4741 ttaacaaaag aggggaaaaa cttcttttat gactattctt taccaatgac tgtcttaaaa
     4801 ttaaaacagg ccttgggacg tactacaagg agagaagaac aaaagtcagc ggtaattatt
     4861 ttggatagca gattggttat taaatcttat ggacaaacca tcatgcattc tcttggtaga
     4921 gattttgaaa tttctaagga aaaaattaac aaggtcttga ctgaaatggc taaatttttg
     4981 atataatggt aaagatcatt gtctaaaaat aagatgttga cagtgcgact caagattaaa
     5041 taatagtagt acgttgataa aaggaggagt gatgacctat ttgtcagaac gtgtactcaa
     5101 tatggaagag agtgttactt tagctgcggg agctaaagca agagaactca gagtacaagg
     5161 aagagatatt cttagtttaa ctttagggga gcctgatttc gctactccta aaaacattca
     5221 gcaagcggct attgaggcta taacagatgg acgtgcaagt ttttacacac catcaagtgg
     5281 tttgccggag ttgaagtcag caattaatgc gtattttgaa cgtttttatg gctactccct
     5341 taaacctaat caggtggttg tgggaactgg tgctaagttt atcctttata ctttctttat
     5401 gacagtactg aatcccggtg atgaagtaat tattccgact ccttattggg tatcttatgc
     5461 tgatcagatt aagatggctg aaggtaagcc agtttttgtc acagcaaaag aagttaatca
     5521 cttcaaagta actgtggaac agttagaagc tgttaggact gataagacaa aagtcatact
     5581 tcttaatagt ccatcaaatc ctacaggtat gatttataaa gctgaggagt tggaggctat
     5641 tggaaattgg gcagtcgaac atgatatcct aattttagca gatgatattt acggtcgatt
     5701 ggtttacaat ggcaatattt ttacccctat ttcaagcctt tcagagtcca ttcgaaacca
     5761 aacgatagtc ataaatggag tttctaagac ttatgcgatg acaggttggc gtgttggttt
     5821 tgcagtgggt aatcatgata ttatagcggc aatgtctaaa gtagtcagtc aaacaacttc
     5881 taaccttacg gctgtctcac agtatgctac tattgaggct ttaaatggtt ctcaagagtc
     5941 atttgaaaaa atgaggctag catttgaaga acgccttaat atcatttatc cattgttgtg
     6001 tcaggtccct gggtttgagg ttgttaaacc acaaggtgct ttttatcttt tcccaaatgt
     6061 tacaaaagca atggaaatga aaggatatac ggatgttaca gcattcacgg atgccattct
     6121 tgaagaagtt ggacttgctt tagtaacggg tgctggtttt ggagcgccgg aaaatgttcg
     6181 tctgagttat gctacagatc ttggaaccct taaagaagct gttcgtagac tgcatgtctt
     6241 tatgggaagt aatgaaatta attaaataaa aatagagaga aacttatgtc aaaaaaactt
     6301 atttcaattg ttgatgttaa agattatgtt ggtcaagaag ttactattgg tgcttgggtt
     6361 gctaacaaat caggtaaagg taagattgcg tttgtacagt tgcgcgatgg ttctgccttt
     6421 ttccaaggtg tcgcttttaa accaaacttc attgaaaaat atggtgaaga atcaggtctt
     6481 gaaaaatttg acgttattaa acgtctcaac caagaaacat ctgtttatgt aacagggatt
     6541 gttaaagagg atgaacgttc aaaatttggc tatgagcttg atattactga ccttgaagtc
     6601 attggagagt cacatgaata tcctataact cctaaagagc atgggacaga cttcttgatg
     6661 gacaaccgtc atctttggtt gcgttctcgt aaacaaatgg ctgttatgca aattcgtaac
     6721 gctattattt attcaactta tgaattcttt gaccaaaatg gttttatcaa atttgatagc
     6781 ccaattttat cagaaaatgc agcagaagat tcaacagagt tattcgaaac agactatttt
     6841 gggaaaccag catttcttag ccaatctggt caattatacc ttgaagctgg agcaatggct
     6901 ctaggccgag ttttcgactt tggtccagtc tttcgtgctg aaaaatctaa aacacgtcgc
     6961 catttaacag aattttggat gatggatgcc gagtattctt tcttgtcaca tgaggaatca
     7021 cttgaccttc aagaagctta tgttaaagca ttgatccaag gcgttctaga tcgtgcgcct
     7081 caagcacttg atattttaga acgtgatgtt gaggctctca aacgttatat cgcagagcca
     7141 ttcaagcgtg tttcttatga tgatgctatt acacttcttc aagagcatga agcagatgaa
     7201 gatacagatt atgaacattt agagcatggt gatgactttg gttcaccaca tgaaacttgg
     7261 atttctaatt actttggagt gccgacattt gtagtgaatt acccagcaag ctttaaagca
     7321 ttttatatga aaccagttcc tggaaatcca gaacgcgttc tttgtgctga tcttttagct
     7381 ccagaaggtt atggagagat cattggcggc tcaatgcgtg aagacaacta cgatgctctt
     7441 gtggctaaaa tggatgaact aggtatggat aaatcagaat acgactttta ccttgacctt
     7501 cgtaaatacg gctctgttcc tcatggcggt tttggtatag gtattgaacg tatggtaact
     7561 tttgttgctg gtaccaaaca tattcgcgaa gctattccat tcccacgtat gttacatcgt
     7621 atcaaaccat aataaaaaag cgctagctag tgcgcttttt tattatggtc tttagaatcg
     7681 ttggttaatt aaattgggac tatgtttttt taacaatgtg attgtcgaaa agacaaggat
     7741 aagactgatt agtcgatcaa tataatctgt tccaacttga acaagtatca aactacttgt
     7801 catgtctaat ccaattccat gtaagaattg tgctataata cttgagccac tagaggtaat
     7861 tcctttaaaa aggataactg ttataactga agcaataata gttcctggta aagaaataat
     7921 cagtgatttc cacaataggc tagagggttt acaattcctt ttaatcaaga taccagtaat
     7981 gatagcaact acgatggcga ctggtgaata ataaagtgaa aaaatatcag tagtcatcca
     8041 attgatgagt gcactgagaa aagcagtgct ggcagcgtac catggtccca ggataactgc
     8101 tattagcaat gtacctatag tatctagata gataggtagg cgtaaaaata gagctaaatt
     8161 agccccaata aggttgatgg cgatagcgat tgagataaat gaaattaaac gagcaggtga
     8221 ttttgtcatt tgttaagtcc taacatttct aagtcttcaa tgatgactga attttgtgca
     8281 tgtaatagga cggtcataaa atcattccaa aataaattag tattgactga ggttaaaatc
     8341 ttactgttag catctttttt ataaaaatga tactgatcta caattgtttg tcccatggcg
     8401 attccgtgac aagcaacatc ggtatagcta tcaaaaccag tagcgatatt ttcattaaca
     8461 aagtaagcaa tagctaaagg atcatttatc acacatccaa taatatgctc atattgccag
     8521 tgaaaatcga agtagaactt tgtgattttt tggataaaag ttgatacgtc tggattaata
     8581 cgctccatat aagataaatg attgggggtt aatacaatat gacgtgtgat gtctagtcca
     8641 accatctcta tttttttgtc aaggttttca aatacatatt gtgcagcatg gggatcatac
     8701 caatagttgt attcagcaac tggagaacaa ttgccatgtg atttaaaatt tcctcccatg
     8761 ctaacaaatc gcttgcaatg tttgcctaat ttgggattag tttgtaaggc tcgagcgata
     8821 ttggtcagag gtccgagagc tataatagaa gtatcatttt tgtgttcaaa atagtttgct
     8881 aaaaaacaat ctgctgattc ttcttgaaag ataattggtt gcgccaatgt aaagttattc
     8941 tctccaaggc catccattcc atgagtatct tgagcagata caaattcacg ttgtaaaggc
     9001 aagttattac caacataaac tgaaatgtcg cgacgattta gtaattcaag cgttacaaag
     9061 gtattcttta gtcctagctc aacaggcgaa ttgcctgctg ttatagtaat agcaacaact
     9121 tctaacttag gatgctgaat agcatacatc agtgctaagg tatcatcaat accgggatcg
     9181 caatcgataa taattttttc tttgttcatt tgtctccttt cttttaatat tagtgaatgt
     9241 tctatgcttt tatgaaacta ttaacaatag tcatggcaat aaaaaagatc ttggcagtta
     9301 ccaagatcac acgaaaaaat gaatttagtt ttctaaattc aatcatattt tcagtcactt
     9361 agttttttat tctgggaagt tttcgaacca gtccgagaaa tcctcgatta tgcacgtgtt
     9421 ttattataat atgaaagcac taacttgtaa agattcaaac tgaccattta acagtgtcta
     9481 gattgattgt tttagcattt gaaattaatt ttgaaatcgg acatctgctg tgtgctttgt
     9541 taacgattag cttactatca gataatgaca gtttttcaat actagcaaca gcgttaactt
     9601 ggaaataaaa tcccttacct acttgctttt ccttcatgag ctggcaatta acatcaacac
     9661 gagattttag gtctttgaat cctttttgtt ctaaaatagc ttttatagtt gcatttaaac
     9721 atgttgacca tgctaaagca attaattctt caggattaaa tcctggtaaa tgatttaaag
     9781 ggtgattggt ctccacaatt tttccattgg agagataaac ttttccatca acgccttgag
     9841 tgtttgatga ggttacttca taaagcataa taaactcctt tgggtctagt ttaccaaatt
     9901 tgggagcatt ccgacaagat tttactattt agaatgagtt tcttttcctc tgaagtaaaa
     9961 tcctaattgc tagccatttg tggtataatg gcataaaaca taagagaaag aggtaatcgt
    10021 aatgtcagac gaacaaatca aacttgtgat tgtaacaggt atgagtgggg caggaaagac
    10081 agtggctatt cagtcatttg aagatttagg atattttaca atagataata tgccgcctac
    10141 tctagtgcct aagtttttag aattagcagc acaatcagga gatacaagca agattgctat
    10201 ggtagttgac atgcgtagtc gtcttttctt tagagaaatt aactctattt tggatagcct
    10261 tgaaattaat gataatatta actttaagat tctattttta gatgctactg acaccgagct
    10321 agtgtcacga tataaagaga cacgtcgtag ccaccctctt gctgccgacg gcagagtgtt
    10381 agatggtatt tctctagaga gagaactatt agcaccgctt aaaagtatga gtcagaacgt
    10441 tgtggacact agtgaattaa caccaagaca actacgtaag gtcatttctg aagaattctc
    10501 aaatcaagat agtcaaagtt cttttcgtat tgaagttatg agttttgggt ttaaatatgg
    10561 tatacctttg gatgcggatt tagtattcga tgtgcgtttt ttaccaaatc catactataa
    10621 accagaatta agagataaga caggtttaga tactgaggtt tatgactatg tcatgagttt
    10681 tgatgaatca gacgattttt atgaccatct tcttgcctta ataaagccaa tcttaccagg
    10741 ctatcaaaaa gaagggaaat ctgttttgac tgtggcaatc ggatgtacag gtgggcaaca
    10801 tcgtagcacc gcttttgctc atagattatc agaagattta aaagcagatt ggacagtgaa
    10861 tgaaagccat cgtgataaaa ataagcgtaa ggagacggtg aatcgttcat gagaaaacct
    10921 aaaataaccg ttattggcgg aggaacggga attccagtta ttttgaaaag tcttcgctta
    10981 gaagatgttg agattacagc ggttgtcacg gtggcagatg atggtggttc gtcaggtgag
    11041 ttgcgctcag ttatgcaact aacaccgcca ggagatttaa gaaatgtttt ggttgccttg
    11101 agtgatatgc cgaagtttta cgaacaaatt ttccaatatc gttttgcaga aggtgatggg
    11161 gattttgcag gtcatccttt aggaaatctt attattgctg gtgttgctga aatgcaaggt
    11221 tcaacttata acgctatgca gagtttaaca cagttctttc atacaacagg aaagatatat
    11281 ccatctagtg aacatccgct cactctgcat gctgttttta aagatggcca tgaagtggtt
    11341 ggagagagtc aaatagccga ttataaaggc atgattgacc atgtgtatgt cactaatact
    11401 tataatgaag agactcctac ggccagccgc aaagttgttg atgcaatttt agaaagtgat
    11461 atgattgtgt tgggtccagg gtcacttttc acctctattt tacctaacct agttattcca
    11521 gagattaaac aagctctttt agaaactcgg gcagaggtag cttatgtatg caatattatg
    11581 acacaacgtg gtgaaactga gcattttaca gacgcagacc acgttgaagt attgaaacgt
    11641 catttaggac aagatgctat cgatacagtt ttggttaata ttgaaaaagt gccagagtct
    11701 tatatggaaa acaatcattt tgatgagtat ttggtacagg ttgaacatga tttttcaggg
    11761 cttagaaaac atgccagacg tgtcatctcg tctaactttt taaaattaga aaaaggtgga
    11821 gccttccatc atggtgactt cgttgttgag gaactaatga atttagtagg aacacattta
    11881 tgagttttac agtaaaagtc aaagaagagt tgctaggtca taagagtgaa aataagatgg
    11941 aactgtcagc tattatcaaa atgtcaggta gtttaggatt agctaaccac ggcctcaacc
    12001 tttctattac cacagaaaat gctaaaattg ctcgtcatat ttatagcatg ttagaagaac
    12061 attatcattt gcagccagaa atcaaatacc atcaaaaaac gaacttacga aagaatcgtg
    12121 tgtatactgt ttttattgaa gaaaaagttg atgttattct tgctgatttg aagttggctg
    12181 atgctttttt tggtattgaa actggtattg aacactctat attggacaat gatgaaaatg
    12241 gaagagctta tctcagggga gcattcttat caacgggtac tgttagagaa cctgattctg
    12301 gaaaatatca actagaaatt ttttctgttt atctagacca tgcacaagac ttagctaatc
    12361 ttatgaaaaa atttatgtta gatgcaaagg ttattgaaca taaacatgga gcagtaactt
    12421 atttgcaaaa agcagaagat attatggatt tcttgatagt cattgatgcc atggaagcta
    12481 gagatgcttt tgaagagatt aaaatgatac gtgagacacg taatgatatt aaccgtgcta
    12541 acaacgttga aacagctaat attgctagaa caatcacagc tagtatgaag actatcaata
    12601 atattatcaa gattatggat actattggtt ttgatgcact gcctagtgat ttaagacaag
    12661 tagctcaggt gcgtgtggct catccagatt actctattca acaaatagca gacagtttag
    12721 aaacgcctct gagtaagagt ggcgttaacc atcgcttacg gaaaataaat aaaattgcag
    12781 atgaattata gaaggagata ttagcatggc ttgtacaaca atattggttg gtaaaaaggc
    12841 ttcttatgat ggttcgacta tgatcgctag aacggaagac tctgttaatg gcgatttcac
    12901 acccaaaaaa ttaaaggtaa tgacatctaa agatcaaccg cgtcattaca aatcagtttt
    12961 atcaaatttt gaagtagatt taccagataa cccacttcct tatacttcag taccggacgc
    13021 attgggaaaa gatggtatat ggggtgaagc cggtattaac agtaaaaatg tagcgatgag
    13081 tgctacagaa actattacaa cgaattcccg cgttttgggt gcagatcctt tggtttcaga
    13141 tggtataggg gaagaggata tactcacttt agtgcttccc tatattcagt cagcgcgaga
    13201 aggtgtggag cgtttaggtg ctattttgga aaaatatgga acctatgaat caaatggtat
    13261 tgctttttca gataccgaag aaatatggtg gttagaaaca attggtgggc atcattggat
    13321 tgctcgtcgc gtacctgatg atgtttatgt tactaatcct aaccaactag gaattgatca
    13381 ttttgaattt aataactgtg atgactacat gtgctctagt gatttgaaag agtttatcga
    13441 acaataccat ttagatttga cctattctaa tgagcatttc aatcctcgat atgcttttgg
    13501 tagccaacgt gataaagatc gtcattacaa cacaccaaga agttgggcaa tgcagcgttt
    13561 tttaaatcct gaaattgaac aggatccacg tagtttgttt attccctggt gtcaaaagcc
    13621 ttaccgaaaa attactgttg aggatattaa atatgtgttg agtgatcatt atcaagacag
    13681 tgtgtatgac ccatatggac cagaagggga tgcggtaagt aggagagctt ttcgttcagt
    13741 tggtatcaac cgaactagtc aaacgtctat tctacaattg cgaccaaata aatcacttga
    13801 aacgacaggt gttcaatggt tatcttatgg ctctatgcca tttgcaacca tggtgccgtt
    13861 gtttacacaa gttgagactg taccagacta tttttcgaat acaaccaagg atgcttcaac
    13921 agataatttt tattggacca atcgtttaat tgcagctcta gcagatccac acttttatca
    13981 acatgaagct gatattgaaa gctatatcga gagaacgatg gcccaaggac atgcacatat
    14041 taatggtatt gatagagaag ttgctgagaa taaagagatt gattttcaac agaaaaatca
    14101 agaaatgagt gactatattc aaaaagaaag ccaagaattg ttaaatcgta ttctatttga
    14161 tgcaagtaat ttaatgacaa atcgcttttc aatgggagat taaaaaactc ggaatatttt
    14221 ccgggttttt taagataaaa acgcttgcat aagtatataa aacttgcaaa aaaaaaatat
    14281 cgtgctatac tttactagta aaatattaac cggtaaataa cgggttaaat aaaaaggaga
    14341 aaaaatgaga aagaaatttc ttttattgat aagctttgta gctatgtttg cagcttggca
    14401 acttgttcaa gttaaacaag tttgggctga tagtaaactt aaagtggtaa caacttttta
    14461 cccagtttat gagtttacaa aaaatgtcgt tggtgataaa gctgatgtat ctatgttaat
    14521 taaagcaggt acagagccgc atgattttga accatcaact aaaaacatcg ctgccatcca
    14581 agattcaaat gcttttgttt acatggatga taacatggaa acttgggctc caaaagtagc
    14641 taagtcagtt aaatccaaaa aagtaacaac tattaaaggt actggcgata tgttacttac
    14701 taaaggcgtc gaagaagaag gtgaagaaca tgaaggacat ggtcatgaag ggcatcatca
    14761 tgaacttgac ccacacgtat ggttgtctcc agaacgtgcg atttctgttg tagaaaacat
    14821 ccgtaataaa tttgtcaaag cttatccaaa agatgcagct tcatttaata aaaatgcaga
    14881 tgcttacatt gcaaaattaa aagagcttga caaagaatac aaaaatggtt tgtcaaatgc
    14941 taaacaaaag agttttgtga ctcaacacgc agcgtttggt tacatggcgc ttgattacgg
    15001 tttaaatcaa gttccaattg ctggtcttac tccagatgca gaaccttcat caaaacgttt
    15061 aggcgaatta gctaaataca tcaagaaata taacatcaac tacatttatt ttgaagaaaa
    15121 tgcttcaaat aaagttgcta aaactttagc agatgaagtt ggcgtgaaaa cagctgtgct
    15181 tagtccactt gaaggacttt ctaaaaaaga aatggcagct ggcgaagatt acttctcagt
    15241 tatgagacgt aatttgaaag ttcttaaaaa gacaacagat gttgcaggta aagaagtagc
    15301 tcctgaagaa gataaaacta aaacagttga aacaggttac tttaaaacta aagatgttaa
    15361 agaccgtaaa ttgacagatt actctggtaa ttggcaatca gtatatcctc ttcttcaaga
    15421 tggaacactt gatccagttt gggattacaa agctaaatct aaaaaagata tgactgctgc
    15481 agagtacaaa aaatattata cagcaggtta caagactgac gtagaatcaa tcaagattga
    15541 tggtaaaaaa caccaaatga cctttgtacg taatggtaaa tcacaaacat ttacatacaa
    15601 atatgcaggt tacaaaatct taacttataa aaaaggtaat cgtggagtac gttatctctt
    15661 tgaagctaag gaaaaagatg ctggtcaatt caaatatatc caatttagtg accatggtat
    15721 taaaccgaat aaagctgaac acttccatat cttctggggt tcagaaagcc aagaaaaatt
    15781 atttgaggaa atggaaaact ggccaacata cttcccagct aaaatgtctg gacgtgaagt
    15841 tgcccaagac cttatgtctc attaatattt aaaagatgat atcggatacg atatcatctt
    15901 tttttcttcg tacgtgatag gctcaaacat agaaaaaagc acttctatta gaagtgcttt
    15961 taaaattaat gctatgctta ttgagcagcg tttgcgtctt tgagaccata ttttttgttg
    16021 aaacggtcca cacgtccatc tgcttgagtg aatttttgac gtcctgtata gaatgggtgt
    16081 gaatctgatg agatttccac acggataagt gggtaagttt caccttcgaa ttctactgtt
    16141 tctttagttg atttagttga accactaagg aatttgtaac ctgtagttgt atctaagaaa
    16201 acaactggac gataatctgg atggatgtct tttttcatct ttaaaaattt cctttctgcc
    16261 ataactcgtc tgagtcatag ttattaacca ctctattcta tcaaataaga atagccttga
    16321 caagtgtttt gataaaaaaa gtaaaattat aaagaaagta cttcctggat ttcttggtaa
    16381 atagcttgat tctcttccaa actataagaa ttagctccac tggctaaagg atggccgcct
    16441 ccattatgac gtttagctat ttcattaatg atgtgtgact tactacgtag gcgcacacga
    16501 taatgcccat cgctttgttc aacgaaaatt gcccaacttt ctacaatatc aatttttcca
    16561 ggtataccaa cgattgcagc agtctctgca tctgttacat caaagcgttt taagtcttct
    16621 tgtgttaaag taacgcatgc agcgccgttc ttgtcaattt caagttgctc aaaaataaag
    16681 ccttgtaatt ttgcaatttt gaaaggaaag gaatccattt gacgtgccat tgcagaaaaa
    16741 tcaaagtcaa attcacgaag tttgctagca atcttcagtg ttttacttgt ggtagcagga
    16801 tataaaaagc gtcctgtatc tcctacaata ccattatata gaactcttgc tgcacttgtt
    16861 gataacaaca aatcacaaga aagagcaaag tctgtcacaa tttcacttgc actagaggcg
    16921 ttagtatcta cataagagag gtctccatag acttcatcgt ttggatgatg gtcaatttta
    16981 attaaaaagt cacctttttt ataacgttca tcatcaatgc gaggggtatt tgctgtatct
    17041 gtaacaataa caagagcgcc ttgatagtct tggtcggtca cttgatccat tttagcaatc
    17101 caagcgagtg tgggttcgtc aaaaccagta gcaagtactt ttttctttgg gaaattatgc
    17161 ctgatgatat cccttaagcc gatttggcta ccaagagcat cagggtctgg tctcatatgt
    17221 cgatggataa tgatggtgtc gtactctttg attttatcaa gaatttgttg aaaaataatc
    17281 attttttacc tacctaactg ttttgtcttt tagtgaagaa caagg
//
LOCUS       DATROD010000029        17218 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.029, whole genome shotgun sequence.
ACCESSION   DATROD010000029 DATROD010000000
VERSION     DATROD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17218
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.029"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            92..844
                     /locus_tag="VBC74_000945"
     CDS             92..844
                     /locus_tag="VBC74_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6941908.1"
                     /translation="MFNKIGFRTWKSGKLWLYMGVLGSTIILGSSPVSAMDSVGNQSQ
                     GNVLERRQRDAENKSQGNVLERRQRDAENKSQGNVLERRQRDVENKSQGNVLERRQRD
                     AENKSQGNVLERRQRDVENKSQGNVLERRQRDAENKSQGNVLERRQRDADNKSQVGQL
                     IGKNPLFSKPTVSRENNHSSQGDSNKQSFSKKVSQVTNVANRPMLTNNSRTISVINKL
                     PKTGGDQNVIFKLVGFGLILLTSRCGLRRNEN"
     gene            1052..2128
                     /locus_tag="VBC74_000946"
     CDS             1052..2128
                     /locus_tag="VBC74_000946"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEO6941909.1"
                     /translation="MGYTVAIVGATGAVGTQMIRQLEQSNLPIEQVKLLSSSRSAGKI
                     LHFKDEAIRVEETTKESFYDVDIALFSAGGSISAKFAPYAVKSGAVVVDNTSYFRQNP
                     DVPLVVPEVNAHAMIGHNGIIACPNCSTIQMMIALEPIRQKWGIERVIVSTYQAVSGS
                     GARAVEETKEQLRQVLNDNLSPDQLIATVLPCSSDQKHYPIAFNALPQIDIFTDNDYT
                     YEEMKMTLETKKIMEDATIKVSATCVRIPVLSGHSESIYIETKELASISEIKKAIANF
                     PGAVLQDLPSQQIYPQAINAVGHRETFVGRIRKDLDQENGVHMWVVSDNLLKGAAWNS
                     VQIAETLHKNGLVKPAKELKFELI"
     gene            2298..2636
                     /locus_tag="VBC74_000947"
     CDS             2298..2636
                     /locus_tag="VBC74_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941910.1"
                     /translation="MQIIKRDGQIVEFDPEKIYQAILKAAQTVYVLDESLRQDLAQVT
                     KKVILDLEDAQVERPTINMIQSLVENRLLDAGYIHIAEHYISYRLQRDLERNGYDDQI
                     TVHLHFEKIK"
     gene            2767..4308
                     /locus_tag="VBC74_000948"
     CDS             2767..4308
                     /locus_tag="VBC74_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO6941911.1"
                     /translation="MSILEVKNLSHGFGDRAIFENVSFRLLKGEHIGLIGANGEGKST
                     FMSIVTGHLQPDEGKIEWSKYVTAGYLDQHTVLEEGQTVRDVLRTAFDELFKTEARIN
                     DIYISMADDGADVDSLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDSDVT
                     ELSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQDLVRYSGDYTNFESVYAMKKAQLEAAYERQQKEIADLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPSFEFKESRTPGRFIFQAKDLQIGY
                     DRALTKPLNLTFERNQKIAIVGANGIGKTTLLKSLLGIIPPISGNVERGDFIDLGYFE
                     QEVPGGNRQTPLEAVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQSKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKAYKGSILMVCHEPDFYEGWMDDVWD
                     FNQLS"
     gene            4348..5580
                     /locus_tag="VBC74_000949"
     CDS             4348..5580
                     /locus_tag="VBC74_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEO6941912.1"
                     /translation="MQNDMSVVLRAGKLLIESGAEVYRVEDTMKHFAKALQIENFEAY
                     VVSSSIIASGINRYGKQEAKVCNTDGVTANLGRLEAVNNLSRQIAKQDLVSPEEIVKQ
                     LDLIEHQKDYSLLVTLISYFCGAGSFSLALGSSLLDSFSAAVTGLILGYFLNLMESRI
                     HTGFLLTILGSSVVALSANLLYFSGLGEHRSIIILGALMVMVPGAAFVNSVREFSQNN
                     FSTGLALIMSALLICISISAGVAITIEIIPFAEQMTGSFSGVPNTILEIIIRTLMAGL
                     GTIAFSILYHVPKRYFLDLGILGAISWMLYLILWQQFHMDAIAVFFPGLFITYFSRLL
                     AAKRKCPATVFLATSMFPLIPGLSFYRAVYFLLTGADALAMEYFRSCFVTAFTIAIAI
                     SIVQQIPLSFFIRRKMIK"
     gene            5793..6494
                     /gene="pyrF"
                     /locus_tag="VBC74_000950"
     CDS             5793..6494
                     /gene="pyrF"
                     /locus_tag="VBC74_000950"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEO6941913.1"
                     /translation="MLEKCPIIALDFSDLASVTTFLEHFPKEELLFVKIGMELYYSEG
                     PSIIRYIKSLGHRIFLDLKLHDIPNTVRLSMSVLAKLGIDMTNVHAAGGVEMMKAARE
                     GLGEGPILLAVTQLTSTSQEQMQVDQHINLSVVDSVCHYAQKAQEAGLDGVVASAQEV
                     KQIKKQTNEHFICLTPGIRPPQTNQLDDQKRTMTPEQARIVGADYIVVGRPITKAENP
                     YQAYLDIKEEWNRIK"
     gene            6507..7136
                     /locus_tag="VBC74_000951"
     CDS             6507..7136
                     /locus_tag="VBC74_000951"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEO6941914.1"
                     /translation="MDLARQIAMELLDIQAVYLRPQQPFTWASGVKSPIYTDNRVTLS
                     YPETRTLIENGFVKQIQKHFPNVDIIAGTATAGIPHGAIIADKMNLPFAYIRSKAKDH
                     GVGNQIEGRVYSGQKMVIIEDLISTGGSVLEAVTAAQSQGIEVLGVVAIFTYQLAKAE
                     QAFREADIPLVTLTDYNQLIKVAKVNGYITADQLVLLKKFKEDQMNWQS"
     gene            7148..8440
                     /locus_tag="VBC74_000952"
     CDS             7148..8440
                     /locus_tag="VBC74_000952"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEO6941915.1"
                     /translation="MYIIKNGLIIDPQSGFNQVSDMLIDQGKIKQISKEIDIKGIPII
                     DASNKIVAPGLVDIHVHFREPGQTHKENIHTGALSAAAGGFTTVLMMTNTNPTISSSE
                     IVKQVKESAAKEAIKIETVATITKSLNGKDLVNFEELLEAGVAGFSDDGIPLTDTKVL
                     QEAMNLARKHDVVLSLHEEDPSLNGVLGINEHIAQKIYHVCGASGLAEYSMIARDAMI
                     AYQTQAKVHIQHLSSSESVEVVDFAQKLGANLTAEVTPQHFSKTEDLLLTKGANAKLN
                     PPLRLEKDRQALIDGLKSGVISIIASDHAPHHIMEKAADNISQAPSGMTGLETSLALG
                     ITYLVSTKELSMIDFLAKMTCNPAQLYGFDAGYLREGGPADIVIFDQAEERIIKAEFA
                     SKSSNSPFIGDKLKGVVHYTICNGEIVYQKDSHAATLV"
     gene            8605..9528
                     /locus_tag="VBC74_000953"
     CDS             8605..9528
                     /locus_tag="VBC74_000953"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEO6941916.1"
                     /translation="MNHQTQTLSLEHFVSLEELSNQEVMSLIKRSIEVKENPSNIGFD
                     KDYYVSNLFFENSTRTHKSFEMAELKLGLKTIEFNADTSSVNKGETLYDTILTMSALG
                     LDVCVIRHPDIDYYKELIASPNIHSAIVNGGDGSGQHPSQSLLDLVTIYEEFGYFKGL
                     KIAIVGDLTHSRVAKSNMQVLKRLGAEIFFSGPKEWYSSQFDEYGQCLPIDQLVDQID
                     VLMLLRVQHERHDGKGVFSKESYHQQFGLTKERYKHLRDTAIIMHPAPVNRDVEIASD
                     LVEADKARIVKQMSNGVYARIAILEAVLNSR"
     gene            9542..10618
                     /gene="carA"
                     /locus_tag="VBC74_000954"
     CDS             9542..10618
                     /gene="carA"
                     /locus_tag="VBC74_000954"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000826106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEO6941917.1"
                     /translation="MKRLLLLEDGSVFEGEAFGADVGTSGEIVFSTGMTGYQESITDQ
                     SYNGQIITFTYPLIGNYGINRDDYESIRPTCKGVIVYEWAEYPSNWRQQMTFDEFLKL
                     KGIPGISGIDTRALTKIIRKHGTMKACLINEGNSIHEALENLQKSVLLNNQIEQVSTK
                     LAYASPGVGKNIVLVDFGLKHSILRELSQRQCHITVVPHTTTAQEILNLNPDGVLLSN
                     GPGNPEQLPNALQMIQEIQGKIPIFGICMGHQLFAKANGAKTYKMTFGHRGFNHAVRH
                     LQTGQVDFTSQNHGYAVSREDFPEALFITHEEINDKTVEGVRHKYYPAFSVQFHPDAA
                     PGPHDTSYLFDEFINMIDDFQQKS"
     gene            10649..13831
                     /gene="carB"
                     /locus_tag="VBC74_000955"
     CDS             10649..13831
                     /gene="carB"
                     /locus_tag="VBC74_000955"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEO6941918.1"
                     /translation="MPKRTDIRKIMVIGSGPIVIGQAAEFDYSGTQACLSLKEEGYQV
                     VLVNSNPATIMTDKDIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMAL
                     SKNGILEELNVELLGTKLSAIDKAEDRDLFKQLMEELNQPIPESEIVNSVEEAIQFAE
                     QIGYPLIVRPAFTLGGTGGGMCDNQEQLVDITTKGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDAADNALVVCNMENFDPVGIHTGDSIVFAPAQTLSDVENQLLRDASLDIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPITKTTYAMFEPALDYVVAKMPRFPFDKFESGDRKLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVDHIKIADLDNVSDDVLLEKIRKAEDDRLFYLAEALRRHYSIEKLAS
                     LTSIDSFFLDKLRVIVELEDLLSKNRLDINILKKVKNKGFSDKAIASLWQINEDQVRN
                     MRKEAGILPVYKMVDTCAAEFDSATPYFYSTYAVENESLISDKASILVLGSGPIRIGQ
                     GVEFDYSTVHSVKAIRESGFEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIDL
                     EKPEGVILQFGGQTAINLAKDLNKAGVKILGTQLEDLDRAENRKQFEATLQALNIPQP
                     PGFTATTEEEAVNAAQKIGYPVLVRPSYVLGGRAMKIVENEEDLRHYMTTAVKASPDH
                     PVLIDAYLIGKECEVDAISDGQNILIPGIMEHIERAGVHSGDSMAVYPPQTLSETIIE
                     TIVDYTKRLAIGLNCIGMMNIQFVIKDQKVYVIEVNPRAGRTLPFLSKVTHIPMAQVA
                     TKVILGDKLCNFTYGYDLYPASDMVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     INLQKALYKAFEAAYLHMPDYGNIVFTVDDTDKEEALELAKVYQSIGYRIYATQGTAI
                     YFDANGLETVLVGKLGENDRNHIPDLIKNGKIQAVINTVGQNNIDNHDALIIRRSAIE
                     QGVPLFTSLDTAHAMFKVLESKAFTLKVLD"
     gene            13957..14280
                     /locus_tag="VBC74_000956"
     CDS             13957..14280
                     /locus_tag="VBC74_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941919.1"
                     /translation="MLKTRTHINELETKIALDLVHRYLGIQQLVFSKWRHSLETGSWS
                     SIITINGANIINYIVDDISDSDSISGIICHPKSFHLRPREKKEPIQSIDELDIIWSLN
                     REENE"
     gene            14277..14747
                     /locus_tag="VBC74_000957"
     CDS             14277..14747
                     /locus_tag="VBC74_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
                     /protein_id="HEO6941920.1"
                     /translation="MIPDSSFKVLSDMAYNIEKSKAESNGHLKIIEGSQIIDFHQKRS
                     FMVLEVNDNIDNGMQAMACQSLDESNHIVIAYSGTNAFNKKDILTDIDSVVLGKNNKL
                     LTCPSSITLREDGISLPCKNPSQITTSKLFAQKIKMNYPDATLWLTGHGKCSVR"
     gene            14725..17199
                     /locus_tag="VBC74_000958"
     CDS             14725..17199
                     /locus_tag="VBC74_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage infection protein"
                     /protein_id="HEO6941921.1"
                     /translation="MVNVQSGNISNYQKNLLDSATNFQNIFPALVNQYSSSITANESL
                     KKSLEASDNMFNDLMTTQTNTGKDLSSLIEQRHQDSISYEAFSASLLEMNNELLEKQL
                     SDIITQAQKDQETLSSQLNSIMGDDNDNDHNHKENSSAYLNVARQKIQELSEALKSQD
                     NIAKDQSEQLDKIVREGLASYFAKNNKDNITLLELLKSHSTNEKTLKDFKVKVADFTN
                     SLISSIPSLNLSELHLTQEEKAIQFTSSDSEIIKKVSNERTLYLNTNLLNRLFEARKN
                     RDEAKNKVNQLKLPSSSTRTGEQIVSVESNNPDYRVDTWTVNGKQTRTLDPSQNNNII
                     INSSYQGELSNSSITKPSYTITIGNQKQIVQNDDGEIQRSYLEAEATYQRTLQEVIDA
                     YNTTHELVAKYYIISDGEEPQNLFDQFLNQSVNDTMVDLVKNGITKYLMDENTADAQQ
                     KVKDVMEEVENSQDELADQMAKVTETNVRLTDTIKKQLETLQTINMKVQNITQDQSKV
                     NDSQKTTDQQLSDLKNQLDGLMTSATGVKDMSKSNSQEADQVNQIFTSFNKDVQDAKN
                     SGNKLSTDATDLMANFQKELANNGDFVASFSKVFNTAYKNGVPNDILLNFLSRPVAES
                     ASAVRATENTYRPFTWILLLEVVSLFIAYIFATQNLIKKLTDRYNVNRWLQTDFLNII
                     VISGLSLVIGLALGVISSRSLHVMPEYVPSWFLVMTLFSFLLIHSQYFFIKNFKAVGM
                     GLALFMIISFVYLSNAVGTVATVSGFPKLLKAINPLSILENQLSSYFDNVTTGFIFLI
                     LVLLVDVAFIIMNIFITLNFEAKVKE"
BASE COUNT         5824 a         2758 c         3343 g         5293 t
ORIGIN      
        1 atagattata aaatgtttta atatatataa tctaaataaa aataataata aaatgacttt
       61 taaaatttaa aaaaagtaag gagaaaatta attgttcaat aaaataggtt ttagaacttg
      121 gaaatcagga aagctttggc tttatatggg agtgctagga tcaactatta ttttaggatc
      181 aagtcctgta tctgctatgg atagtgttgg aaatcaaagt caaggtaatg ttttagagcg
      241 tcgccaacgt gatgcggaaa acaaaagtca gggtaatgtt ttagagcgtc gccaacgtga
      301 tgcggaaaac aagagccaag gcaatgtttt agagcgtcgt caacgcgatg ttgagaacaa
      361 gagccaaggt aatgttctag agcgtcgcca acgtgatgcg gaaaacaaga gccaaggcaa
      421 tgttttagag cgtcgtcaac gcgatgttga gaataagagc caaggcaatg ttttagagcg
      481 tcgtcaacgt gatgcggaaa acaaaagtca gggcaatgtt ctagagcgcc gccaacgtga
      541 tgcggataac aagagccaag taggtcaact tatagggaaa aatccacttt tttcaaagcc
      601 aactgtatct agagaaaata atcactctag tcaaggtgac tctaacaaac agtcattctc
      661 taaaaaagta tctcaggtta ctaatgtagc taatagaccg atgttaacta ataattctag
      721 aacaatttca gtgataaata aattacctaa aacaggtggt gatcaaaatg tcatttttaa
      781 acttgtaggt tttggtttaa ttttgttaac aagtcgctgc ggtttgagac gcaatgaaaa
      841 ttaagtataa tcaatcattt agtaactatt ataatgatat atacaatcaa taaaaaggaa
      901 tcggatacga gattcctttt tataattagg ttggttaggg tgactttttt catttggcta
      961 ttcttgaaag tttataaaaa tgtagtataa tagtcacatt aaaatgtttt gaaaatattg
     1021 atgaataaca tcaacaaata gaggtcatta tatgggatat accgttgcta tcgtgggtgc
     1081 tacaggtgcc gtaggaacac aaatgattcg tcaattagaa caatcgaatt taccaataga
     1141 acaagtgaaa cttttatcat caagtcgctc agcaggtaaa attttacatt ttaaagatga
     1201 ggctatacgt gttgaagaga caacaaaaga atcattttac gatgttgata ttgccttgtt
     1261 ttcagctggt ggatctattt cagcaaagtt cgctccttat gcagtaaagt ctggagcagt
     1321 tgtagtagat aacacgtcat attttcgtca gaatcctgat gttccactag ttgttcctga
     1381 agtaaatgct catgccatga ttggtcataa tggtatcata gcttgtccca attgttctac
     1441 tattcaaatg atgattgctt tagagcccat tcgtcaaaaa tgggggatag agcgtgttat
     1501 agtttccacc tatcaagctg tttcgggttc aggtgcacgt gctgttgaag aaactaagga
     1561 acagttgaga caagttttaa atgataacct ttcacctgat caattaattg caacggtctt
     1621 gccatgttct agcgatcaaa aacattatcc tatcgcattc aatgcattac ctcaaattga
     1681 tatttttact gataatgatt atacgtacga agaaatgaaa atgactttgg agacaaagaa
     1741 aattatggaa gatgctacta ttaaggtttc agctacctgc gtccgcattc ccgttttatc
     1801 gggtcattct gagtctattt atattgagac gaaagaatta gcctcaatct ctgaaattaa
     1861 aaaggctatt gcaaattttc ctggtgccgt cctacaagac cttccatctc agcaaattta
     1921 ccctcaagcg attaatgctg taggccatag ggagaccttt gttggtcgta ttcgaaaaga
     1981 ccttgatcaa gaaaatggtg ttcatatgtg ggtagtttca gataatctac tgaagggggc
     2041 agcttggaat tctgttcaaa tcgctgaaac attgcataag aatgggctgg ttaaaccagc
     2101 gaaagagtta aaatttgaat taatatagaa gaatatgaca ttgcttaata cttatactga
     2161 cttctagtga tattaatttt tcatcaatat caggtaaaac acttgacaaa ttatgaaaac
     2221 atttgcataa tgaaattaac aaatgaaaat gattgcaaat cgttttaaca ttgtttccac
     2281 attaaggagg ggctcttatg caaatcatca aacgtgatgg acagattgtt gaatttgatc
     2341 ctgaaaaaat ctaccaagct atcttgaaag ctgctcaaac agtttatgtg cttgatgagt
     2401 cacttcgtca agaccttgct caagtgacta aaaaagtaat cttagattta gaggatgctc
     2461 aggttgaacg accaacgatc aatatgattc agtctttggt tgaaaatcgt ttattggatg
     2521 caggatacat tcatattgct gaacactata tttcataccg tttgcaacgt gatttagagc
     2581 gaaatggtta tgatgatcaa atcactgttc atttacactt tgaaaaaatt aagtaaggtt
     2641 tgacttttaa gtttaaactc tctatacttg tatatggaac ttactatgta atgtgctaat
     2701 ttaaaaactt gtagagtgtc tctatgagtt ttttaataac ctctattatc aatcaaagga
     2761 ttttaaatga gtattttaga agtaaaaaac ttaagtcacg gttttggtga tcgtgctatt
     2821 tttgagaatg tttcttttcg ccttttaaaa ggtgaacata taggacttat tggtgctaat
     2881 ggtgaaggaa aatcaacatt catgtcaatt gttacaggtc atcttcaacc tgatgaagga
     2941 aaaattgaat ggtcaaaata tgtaactgct ggttaccttg atcaacatac tgtgttagaa
     3001 gaaggtcaaa cagttcgtga tgttttgcga acagcatttg atgaactttt taaaacagag
     3061 gcacgtatca atgatatata tatatccatg gcagatgatg gcgcagatgt agatagcctt
     3121 atggaagaag ttggagaact acaagatcgt ttagaatcac gtgattttta tacgcttgat
     3181 gctaaaattg atgaagtcgc acgggcatta ggagttatgg attttggtat ggattctgat
     3241 gttactgaat tatctggtgg tcaaaggaca aaggtccttt tagcaaaact gttattagaa
     3301 aagccagata tcctcctgct tgatgagcct acaaactacc ttgatgctga acacattgat
     3361 tggttaaaac gctatttaca aaattatgaa aatgcttttg ttttaatctc acacgatatt
     3421 ccatttttga acgatgttat caatattgtc tatcatgttg aaaatcaaga tttagtaaga
     3481 tattccggtg attatactaa ttttgaatca gtctatgcaa tgaaaaaggc tcaattagaa
     3541 gcagcctatg aacgtcaaca aaaggaaata gctgatttac aagattttgt taatcgtaat
     3601 aaagcacgcg ttgcaacacg taatatggca atgtctcgtc aaaagaaact agacaaaatg
     3661 gatattattg agttacaagc agagaaacca aagccaagtt ttgaatttaa agaatctcgc
     3721 acaccaggac gttttatttt ccaagctaaa gatcttcaaa ttggatatga tcgtgcttta
     3781 acaaaacctc taaacctaac atttgaacgt aatcaaaaaa tagctattgt tggagcaaat
     3841 gggataggaa aaacaacatt acttaagagt ttactaggta ttatcccccc tatttctggt
     3901 aatgttgaac gaggtgattt tattgattta ggttattttg aacaagaagt acccggaggg
     3961 aatcgccaaa ctccacttga agcagtatgg gatgcttttc ctgctttgaa ccaagcagag
     4021 gttagggcag ctcttgctcg ttgtggttta acttcaaagc atattgaaag tcagattcag
     4081 gtgttatctg gtggggagca atcaaaggtc agattttgct tattaatgaa tcgcgaaaat
     4141 aatgtccttg tacttgatga accaaccaat catttagatg tagatgctaa agatgagtta
     4201 aaacgtgctt taaaagctta taagggatca atcttgatgg tatgtcatga accggacttt
     4261 tatgaaggat ggatggatga tgtttgggac tttaaccaac ttagttaagt ggctagggta
     4321 ataaagtaga aggcagagcc aaataagatg caaaatgata tgagcgttgt tcttagagct
     4381 ggtaaattac tgattgaaag cggagcagaa gtctatcgtg tcgaagacac aatgaagcat
     4441 tttgctaagg ctcttcagat agaaaatttt gaagcttatg tggttagtag cagtattatc
     4501 gcttccggga ttaaccgcta tggtaaacaa gaagctaaag tatgcaatac agatggtgta
     4561 acagcaaatt taggtagact tgaagctgtt aataaccttt ctaggcaaat tgctaaacaa
     4621 gacttagtat ctccagaaga aattgtaaag cagttagact taattgaaca tcaaaaagat
     4681 tatagtttgc ttgttacttt aatttcttat ttttgtggag cgggtagttt ttctttagcc
     4741 ttgggtagtt ccttattaga ttcattttcc gctgctgtaa caggcttaat tttaggatat
     4801 tttttgaatt tgatggaaag tcgtatacat acaggatttc ttttgaccat tctaggtagt
     4861 tctgtagtgg ctttaagtgc taatttatta tatttttcag gattaggtga gcatcgtagc
     4921 attatcatat taggggcatt aatggttatg gtgccagggg ctgcctttgt taattcggtg
     4981 agagaatttt cacagaataa tttttcaaca ggtttagctt tgattatgtc tgctttactc
     5041 atttgtattt ctatatcagc aggcgtggct attacaattg aaattattcc gtttgcagaa
     5101 caaatgacag gttctttttc tggtgttcct aataccattc ttgagattat catcagaaca
     5161 ctaatggccg gtttagggac gatagccttc tctattctct accatgttcc aaaacgttat
     5221 ttccttgact tagggatact aggagcaata tcttggatgc tctacctgat cttatggcaa
     5281 caatttcaca tggatgctat agccgtattt tttccagggc ttttcatcac ttatttttca
     5341 cggctattag ctgctaaacg taagtgtcct gccactgtct ttttagcgac aagtatgttt
     5401 cctcttatcc caggacttag cttctataga gccgtttact ttttattgac gggagcagat
     5461 gccttagcaa tggaatattt tagatcctgt tttgtaacag cttttacaat tgctatagca
     5521 atcagtattg tacagcagat tcctctatcg ttctttatta gacgtaaaat gataaaataa
     5581 tttagtattt atttgacaaa atgattgtaa acttgtagaa tacctacaaa tgtatcttta
     5641 agtgagtcca gagaggcgaa caaggtcacc atcataagtc aaatagcgag tgctttttcg
     5701 ctaacttttt atgaaacctt gccctcaggc aaggtttttt gtgtcatact ggtacatttc
     5761 caacaattat tatttctata gagaggaatt atatgttaga aaaatgccct atcattgcct
     5821 tagatttttc tgatttggct tctgtaacaa cttttttaga acattttcca aaagaagaat
     5881 tgctttttgt caaaatagga atggagcttt attattccga aggtccctca attattagat
     5941 atattaaatc cttaggacat cgcatttttt tggatttaaa attacatgat atccctaata
     6001 cagttcgatt atctatgtct gtcttagcca aattaggcat tgatatgact aatgtccatg
     6061 ctgcaggagg agtcgaaatg atgaaagcag ctagagaagg tttgggggag ggtccaatac
     6121 tattagcagt gacacaatta acctcgactt cacaagaaca aatgcaggtg gatcaacata
     6181 ttaacttaag tgtagttgat tcggtttgcc attatgctca aaaagctcaa gaagcaggct
     6241 tagatggagt agtcgcatcg gctcaagagg taaagcaaat caagaaacag actaacgaac
     6301 atttcatttg cttaacgcct ggtatcagac ctccacagac caatcagtta gatgatcaaa
     6361 aaagaacgat gactcctgag caagcacgta tagttggggc tgattatatc gttgtgggaa
     6421 gacctataac gaaagcagaa aatccatatc aagcatatct agacataaaa gaagaatgga
     6481 atcgtattaa ataaaggagt actgagatgg atttagcaag acagattgcc atggaactat
     6541 tagatattca agctgtttac ttaagacccc aacaaccctt tacttgggct tcgggggtca
     6601 aatcgccaat ttacactgat aatcgtgtaa ccttatcgta tcctgagaca aggacactga
     6661 ttgaaaatgg ttttgttaaa caaatacaga aacatttccc aaacgttgat atcatcgcag
     6721 gaacagcgac tgcaggcata ccacatggcg ctattatagc tgataagatg aacttgccct
     6781 ttgcctatat acgctcgaag gcaaaggatc atggtgtggg gaatcaaatc gagggacgtg
     6841 tttactcagg ccaaaaaatg gttatcattg aagatttgat ttcaacagga ggatctgttt
     6901 tagaggctgt tacggctgcg cagagtcaag gtattgaggt tttaggggtg gtagcgatat
     6961 ttacatatca attagctaag gctgaacaag catttcgtga agctgatatt cctttggtga
     7021 ctttgacgga ttacaatcaa ttaattaaag ttgctaaagt taatggatat attacagctg
     7081 accaactggt attactgaaa aaatttaaag aagatcaaat gaattggcaa agttagagag
     7141 gacagatatg tatatcatta aaaacggatt gattatcgac cctcaaagtg gttttaatca
     7201 agtatcagac atgctgattg atcaaggaaa aattaaacaa atatcgaaag aaattgatat
     7261 taaaggcatt ccaatcattg atgcaagcaa taaaattgta gctccaggat tagtcgatat
     7321 tcatgtacat tttcgtgagc ctggacagac acataaagaa aatatccata caggagcctt
     7381 atcagctgca gcaggtggtt ttacgacagt tcttatgatg accaatacta acccaacgat
     7441 ttctagctca gaaattgtaa aacaagttaa ggagagtgca gcaaaagaag caattaagat
     7501 tgaaacagtt gctacaatta ctaaatccct gaatggtaaa gatttagtta atttcgaaga
     7561 attattagaa gctggagttg ctggattctc agacgatggt atccctttga cagatactaa
     7621 ggttttgcaa gaagctatga acttagctag aaaacatgat gttgtcctat cgttacatga
     7681 agaggatcca tcgttaaatg gtgttcttgg tatcaacgaa catattgctc agaaaattta
     7741 tcatgtctgt ggagctagtg gtcttgctga atattcaatg attgcgcgag atgctatgat
     7801 tgcttaccaa acacaggcga aagtccatat ccagcatttg tcaagttcag aatcagtaga
     7861 agtagttgat tttgcgcaga agcttggtgc taacttaaca gcagaagtaa ccccccaaca
     7921 tttttcaaag acagaggact tacttttaac taagggtgct aatgcaaaat taaatccacc
     7981 actgcgactt gaaaaggata gacaagccct cattgatggt cttaaaagtg gtgttatttc
     8041 aattattgca agtgatcacg ctccccatca tattatggag aaagcagcag ataatattag
     8101 tcaagcgccc tctggtatga ctggcttaga gacatcatta gccttaggga ttacttattt
     8161 agtatctact aaagaactct caatgattga ctttttggca aaaatgacat gtaatcctgc
     8221 acagttatat ggatttgatg caggttatct cagagaaggt ggacctgcag atatcgttat
     8281 ttttgatcaa gctgaagaga gaataataaa ggcagaattt gcctccaaat ccagcaactc
     8341 tccttttatc ggtgataaat tgaagggagt tgttcactac acaatttgca atggtgaaat
     8401 agtctatcaa aaagatagcc acgcagcgac attggtatag gtaaaacatt tcttattaat
     8461 atcctttaag gtattgtcca gagaggcaaa caaggaattg cgttaacaaa tagtaagtta
     8521 gttccctatc tagcttacta ttttgcagag cctccttgtt ttaggaggct tttttctagc
     8581 taaaatttat agaaagaaat tatcatgaat catcaaacac aaacactttc attagaacat
     8641 tttgtcagtt tagaagaatt atcaaatcaa gaagttatgt ctttgataaa gagaagtatc
     8701 gaagttaaag aaaatcctag caacattggt tttgataaag attactatgt gtcaaatctc
     8761 ttttttgaga attcaacaag aacacataag tcatttgaaa tggcagaact caaattagga
     8821 ttaaaaacaa ttgaatttaa tgctgataca agttctgtta ataaaggaga aaccctttat
     8881 gacactatct taacaatgag tgctctagga ttggacgttt gtgtcattag gcaccctgat
     8941 attgactatt ataaagaatt gattgctagt ccgaacattc attcagctat tgtcaatggg
     9001 ggtgatggct caggccaaca tccaagccaa tcacttttgg atttggtaac tatttacgaa
     9061 gaatttggat acttcaaagg actaaaaatt gcgattgtag gagatctaac tcactcacgg
     9121 gtagctaaat ctaacatgca agtgctaaaa cgattaggag ctgaaatttt cttttctgga
     9181 cccaaagaat ggtatagtag tcagttcgat gaatatgggc aatgtttacc aatagatcag
     9241 ttagttgatc aaattgatgt ccttatgctt ttacgtgttc aacacgaacg tcatgatggt
     9301 aaaggggtgt tttcaaaaga aagctatcat caacaatttg gtcttacaaa agaacgctat
     9361 aaacatttaa gggatacagc aattattatg cacccagcac ccgtcaatag ggatgtagag
     9421 attgctagcg atttagtaga agcagataag gctcgaatag tgaaacagat gagtaatggc
     9481 gtctatgcaa ggatagctat cttagaagca gtgctcaata gcagataata aaggagataa
     9541 aatgaagcgt ctattattat tggaagatgg tagtgtgttt gagggtgagg ctttcggagc
     9601 ggatgtagga acaagtggtg aaattgtttt tagtacagga atgacagggt atcaagaatc
     9661 tataacagat caatcttaca acggtcaaat cattactttt acttatcctc ttattggaaa
     9721 ttatgggata aatcgagacg attacgaatc tattagacca acatgtaagg gagtaattgt
     9781 ttatgaatgg gcagagtatc caagtaactg gcgtcaacag atgacatttg atgaattttt
     9841 aaaattaaaa ggaatacctg gaatttcagg tatagatact cgagcattaa caaaaattat
     9901 tcgaaaacat ggaacaatga aagcttgttt aataaacgag ggaaattcta ttcatgaagc
     9961 actagaaaac cttcaaaaaa gtgttctttt aaataatcaa attgaacaag tatcaaccaa
    10021 attagcttat gcttcgcctg gagttggcaa aaatattgta ttagttgact ttggtttaaa
    10081 acattctatt ctaagagaac tgtcacaacg tcaatgtcat attacagtcg tccctcatac
    10141 tacgacagcg caagaaatct taaatctcaa tccagatgga gtactcttat ctaacggccc
    10201 tgggaaccca gaacaattac cgaatgcttt acaaatgatc caagaaattc aaggtaaaat
    10261 tccaattttt ggtatttgta tgggacatca actatttgct aaagccaatg gtgcaaagac
    10321 ttataaaatg acttttggtc atcgaggttt taaccatgct gttcgtcatt tgcaaacagg
    10381 acaggttgat tttacaagtc aaaatcatgg ttatgctgtc tcgagagaag atttccctga
    10441 ggctctcttc attacacatg aagagattaa tgataaaacc gtcgaaggtg ttcgacataa
    10501 atactatcct gccttttcag tgcagtttca cccagatgca gcaccagggc cgcatgatac
    10561 atcttatctt tttgatgaat ttattaatat gattgatgat ttccagcaaa aaagctagct
    10621 ttaaaatata gaattagagg agtttgatat gccaaagcgc acagacatac gtaaaattat
    10681 ggttatcggt tctggcccga ttgtgattgg acaagcagca gagtttgact attctggtac
    10741 acaagcctgt ctctctttaa aagaggaggg ataccaagtt gttttggtca attcgaatcc
    10801 ggctactatc atgacagata aagatattgc tgataaagtt tatattgaac ctattacctt
    10861 ggagtttgtc acaagaattc tcaggaagga aagacctgat gctctccttc caactcttgg
    10921 aggtcaaact ggattgaata tggctatggc tctatcaaaa aatggtatcc tagaagaatt
    10981 gaatgttgaa ctattaggga ccaaattatc agccattgat aaagctgaag atcgtgattt
    11041 atttaaacag ttgatggaag aactcaatca acctattcca gaatccgaga ttgttaattc
    11101 ggtagaagaa gctattcaat ttgcagagca aatcgggtat ccattaattg ttcgtcctgc
    11161 ctttacttta ggaggtacag gtggaggcat gtgcgataat caagaacaat tggttgacat
    11221 cacgacgaag gggttaaagt tatcgcctgt gacacagtgc cttattgaac gttcaattgc
    11281 tggctttaaa gagattgaat acgaagttat gcgtgatgca gcagataacg ctcttgttgt
    11341 ttgtaatatg gagaattttg atcctgttgg tatccataca ggggactcta tagtttttgc
    11401 acccgcacaa acgttatctg atgtggaaaa tcagttatta cgagatgcga gtttagatat
    11461 tataagagct ttgaaaattg aaggaggatg caatgttcaa ctagcccttg atccgaatag
    11521 ttttaaatac tatgtcattg aagttaatcc aagagtatca agatcttctg ctttagcttc
    11581 aaaagctact ggatatccaa ttgctaaatt agcagctaag atagctgttg gtttgacact
    11641 agatgaggta attaatccta ttacaaaaac gacttacgct atgtttgaac ctgctttaga
    11701 ttacgtagtc gcaaaaatgc cgagatttcc ttttgataaa tttgaaagtg gcgatagaaa
    11761 attgggaaca cagatgaaag ctacaggcga agtcatggca ataggacgga atatagaaga
    11821 atctttgttg aaagcttgtc gttcactaga aataggtgtc gatcacatta aaatagcaga
    11881 tttagataat gtatcagatg atgttttatt agaaaaaata agaaaggcag aagatgatcg
    11941 cttattttat ctagcagaag ctttacgtag acattatagt attgaaaaat tagcaagttt
    12001 gactagcatt gattctttct tccttgataa gttaagagtg attgtagagt tagaagatct
    12061 attatctaaa aacagactgg atatcaatat tctcaaaaag gttaaaaata aaggtttttc
    12121 agataaagct atagcgagtt tatggcaaat aaacgaagat caagttcgta acatgcgaaa
    12181 agaagcagga attcttccag tttataagat ggttgataca tgtgcggctg agtttgattc
    12241 agcaacgccc tacttttatt cgacttatgc cgtagaaaac gagtcattga tatcagataa
    12301 agcctctatt ttggttttag gatcgggccc aatccgaatt ggacagggag ttgaatttga
    12361 ttattcaacg gttcattctg ttaaagccat cagagagtct ggttttgaag cgattatcat
    12421 gaactctaat ccagagacgg tctcaacgga tttctctatt tcagataagc tttactttga
    12481 acctttgact tttgaagatg ttatgaatgt tattgactta gaaaaacctg aaggggtcat
    12541 cttacaattc ggtggtcaga ctgctattaa tctagcaaaa gatttaaaca aggcaggggt
    12601 taaaatacta ggcacacaat tagaagattt ggatcgtgct gaaaatagaa aacaatttga
    12661 agccacttta caagctctaa atatccctca accaccaggt tttaccgcca ctacggagga
    12721 agaggcagtt aatgcagcgc aaaagattgg ttatcccgtg ttggttagac catcatatgt
    12781 tcttggtgga cgagctatga agatagttga aaacgaagaa gatttacgtc attatatgac
    12841 aaccgcggta aaggcaagcc cagatcatcc agtgcttata gatgcatacc ttataggtaa
    12901 agagtgtgag gttgatgcta tatccgatgg tcagaatata ctgattcctg gaataatgga
    12961 acatatagag cgagctggtg ttcattcagg agattccatg gctgtatacc ctcctcaaac
    13021 attgtctgag accattatcg aaactattgt agattataca aaacgcttag caattggatt
    13081 aaactgtatc ggtatgatga atattcagtt tgtaatcaag gatcagaaag tatatgttat
    13141 tgaagttaat cctcgtgctg gtagaacact tccattctta tcaaaagtaa ctcacattcc
    13201 tatggcccaa gtagcaacaa aagttatctt aggagataaa ctctgcaatt ttacatatgg
    13261 ttatgatctc tatccagctt cagatatggt ccacattaaa gcccctgtct ttagttttac
    13321 caaacttgct aaagtagata gtttattagg tcctgaaatg aaatcaacag gcgaagtgat
    13381 gggatcagat attaatcttc agaaagcctt gtataaagcc tttgaagcag catatctcca
    13441 tatgccagac tatggtaaca ttgtttttac agtagatgat acagacaaag aagaagcttt
    13501 agaacttgca aaagtctatc aaagtattgg ttatcgtatt tatgctaccc aagggactgc
    13561 aatttatttt gatgctaatg gtttagagac agttttagta ggtaagttag gagagaatga
    13621 tcgaaatcat attcctgatt taattaaaaa tggtaagatt caagcagtta tcaacacagt
    13681 tgggcaaaat aacattgata accatgatgc tctcatcatc agacgttctg caattgaaca
    13741 aggagttcct ctatttacat ctttagatac tgcacatgca atgttcaaag ttcttgaaag
    13801 caaagcattt acattaaaag tactagatta atcaaacaat caaggtaaaa ttaacttcca
    13861 gcaaaacaga atcggactag attaaatata gtttgttctg tttttataaa aaagcctaat
    13921 tgtgataaaa tagggaacaa acttaaggag ttaattatgt taaaaacacg aacacatatt
    13981 aatgaattag aaacaaaaat cgcccttgat ttagtacaca gatatctagg tattcaacaa
    14041 cttgtttttt caaagtggag acattcttta gaaactggtt catggtcttc tattataaca
    14101 ataaatggcg ctaatattat caattacatt gttgatgata ttagcgacag tgatagtatt
    14161 tcaggtatta tttgtcatcc taaaagtttt catctcagac ctagagaaaa aaaggaacct
    14221 attcagtcta ttgatgagtt agatatcatc tggtctttga atagagagga aaatgaatga
    14281 ttccagatag tagttttaaa gtgctatcag atatggcata taatattgaa aaatccaaag
    14341 cagaaagtaa tggacacctt aaaattatag aaggtagtca aattattgat ttccatcaaa
    14401 aacgctcttt tatggtatta gaggttaatg ataatatcga taatggtatg caggctatgg
    14461 catgtcagtc tttggatgag agtaatcata ttgttatagc atattcagga acgaacgctt
    14521 ttaataagaa agatatctta acagatatcg attcagttgt attggggaag aacaataaat
    14581 tgctaacttg tccttcaagt attaccctga gagaggatgg catttctctg ccttgtaaaa
    14641 atccaagtca aattacaaca tctaaactat ttgcacaaaa aattaagatg aactatcctg
    14701 atgcaaccct ctggttaaca ggccatggta aatgttcagt caggtaatat ctcaaattac
    14761 caaaaaaatc ttttagattc tgcaactaat ttccaaaata tctttccagc cctcgttaac
    14821 cagtatagta gttccattac tgctaacgaa tcactgaaaa aatctttaga agcttctgat
    14881 aacatgttta atgatttgat gacaacccag acaaatactg gaaaagattt atcaagctta
    14941 atagaacagc gccatcaaga tagcatttcg tatgaagcgt tttcggcctc attactagaa
    15001 atgaataacg agttattaga gaagcaatta tctgatatta tcacacaagc acaaaaagac
    15061 caagaaacat tatcatcaca actcaatagt attatgggtg atgataacga taatgatcat
    15121 aatcataaag aaaactcatc agcttatcta aatgttgcaa ggcaaaaaat ccaagaacta
    15181 tctgaagcac tcaagtcaca agataacatc gctaaagatc aaagtgaaca actagataaa
    15241 attgttagag agggactagc aagttacttt gctaagaata ataaagataa tattacttta
    15301 ttagaattat tgaagagtca ttctactaat gagaagactt tgaaagattt taaagttaag
    15361 gtagcagatt tcacaaattc tctcatttca agcattccct cactaaacct ttcggagttg
    15421 cacttaaccc aagaagaaaa agccattcag tttacatctt ctgattcgga aatcattaaa
    15481 aaagtaagca atgaaagaac tctgtattta aatactaatt tattgaatcg tctttttgaa
    15541 gctaggaaaa atagagatga agccaaaaat aaagttaatc aattaaaatt gccatctagt
    15601 tctactcgaa ctggtgagca gatagtttct gttgaatcaa ataatccaga ttacagggta
    15661 gatacttgga cggttaatgg taagcaaaca agaaccttgg accccagcca aaacaataat
    15721 attattatca atagcagtta tcaaggggaa ttatctaact ctagtattac aaaaccaagc
    15781 tatacaatca ctatcgggaa tcagaaacaa attgtacaaa atgatgacgg tgaaattcaa
    15841 cggtcttact tggaggcaga agcaacgtat caacgaactc tacaagaagt aattgatgca
    15901 tataacacca ctcacgaatt agtagctaaa tactacatta tttctgatgg cgaagaaccg
    15961 caaaatttat ttgatcaatt tttaaatcaa agtgttaacg atacgatggt tgatctggtc
    16021 aaaaatggta taactaagta tttgatggat gaaaacactg ctgatgcgca acagaaagtt
    16081 aaagatgtca tggaggaagt tgaaaatagc caagatgaat tggcggatca aatggcaaaa
    16141 gtaactgaga caaatgtacg actaacagat acaatcaaaa aacagcttga gactcttcaa
    16201 acaattaaca tgaaggtcca aaacatcaca caggatcaat ctaaagtaaa cgactcacag
    16261 aaaacaactg atcaacaatt atctgatttg aagaaccagc tagatgggct aatgacctct
    16321 gcaactggcg taaaagatat gtccaaatct aatagtcaag aggcggacca agttaatcaa
    16381 atattcacat cattcaataa agatgttcaa gatgctaaaa attcgggcaa caaactttcg
    16441 acggatgcaa cagatttaat ggctaacttc caaaaagaat tggccaataa cggtgatttc
    16501 gtagcttctt tctctaaagt ttttaatact gcttataaaa atggagtgcc aaatgatatt
    16561 ctccttaatt tcttatcacg acctgtcgct gaatcagcgt ctgcagttag ggcaactgaa
    16621 aatacttatc gcccattcac ttggatacta ttattagaag ttgttagcct atttatagca
    16681 tatatctttg cgactcaaaa ccttattaag aaattgacag ataggtataa tgtcaatcgc
    16741 tggcttcaaa cagacttttt aaatatcatt gtcatttcag gcctctctct tgtgattggt
    16801 ttagcactgg gagttatctc aagcagaagt cttcatgtca tgcctgaata tgtaccatct
    16861 tggttcctag ttatgactct gtttagtttt ttattaattc atagtcagta tttctttatt
    16921 aaaaatttta aagcagttgg tatgggttta gctttgttta tgattattag ttttgtgtat
    16981 ctatcaaatg ccgtaggcac agtagcgact gttagtggat ttccaaaatt actaaaagct
    17041 attaatccac tatctatcct tgaaaatcaa ttatcatctt attttgataa tgtaacaact
    17101 ggatttattt tccttatctt agtacttctt gtggatgttg cttttattat catgaatatt
    17161 tttatcacct taaattttga agctaaagta aaagagtgag gttgttgatg aagttgaa
//
LOCUS       DATROD010000030        16690 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.030, whole genome shotgun sequence.
ACCESSION   DATROD010000030 DATROD010000000
VERSION     DATROD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16690
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.030"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(55..1215)
                     /locus_tag="VBC74_000959"
     CDS             complement(55..1215)
                     /locus_tag="VBC74_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEO6941922.1"
                     /translation="MIPFTIEYIFVLIGAFLLSIILTPIIRVISLKVGAVDKPNARRI
                     NKVPMPSSGGLAIFLSFVVTTLFFMPMAASRHFIEVSYFHYILPVIIGGLVVTTTGFI
                     DDIFELRPRYKMLGIIIAAIIIWKFTHFRFDSFKIPIGGPLLEFGPILTFFLTVLWII
                     SITNAINLIDGLDGLVSGVSIISLATMAVVSYFFLPKIDFFLTLTIVILIASIVGFFP
                     YNYHPAIIYLGDAGALFIGFMIGVLSLQGLKNSTAVAVITPVIILGVPILDTAVAIVR
                     RKLSGKKISEADKMHLHHRLLSMGFTHRGAVLVVYGIAIIFSLIALLLNVSSRIGGIF
                     LLLALLLVMEIFIEGLNIWGENRTPLFNLLKFIGNSDYRQSVIAKYSDKHQK"
     gene            complement(1217..1972)
                     /gene="mecA"
                     /locus_tag="VBC74_000960"
     CDS             complement(1217..1972)
                     /gene="mecA"
                     /locus_tag="VBC74_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEO6941923.1"
                     /translation="MEMKQISETTLKITISMEDLEDRGMELKDFLIPQEKTEEFFYSV
                     MDELDLPENFKNSGMLSFRVTPKKDRIDVFVTKSELSKDLNLEELADLGDISKMSPED
                     FFKTLEQSMLEKGDTDAHAKLAEIENMMDKATQEVVEENVSEEQPEKEVETIGYVHYV
                     FDFDNIEAVVRFSQTIDFPIEASELYKNGKGYHMTILLDLENQPSYFANLMYARMLEH
                     ANVGTKTRAYLKEHSIQLIHDDAISKLQMIEMG"
     gene            complement(2092..2931)
                     /locus_tag="VBC74_000961"
     CDS             complement(2092..2931)
                     /locus_tag="VBC74_000961"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEO6941924.1"
                     /translation="MLIIELLKALFLGVVEGVTEWLPVSSTGHLILVQEFMKLNQSKS
                     FVEMFNIVIQLGAIMAVIVIYFKRLNPFQPGKSAREIRLTWQLWLKVVIACIPSILIA
                     LPFDNWFEAHFNFMIPIAIALIFYGFVFIWVEKRNAHLKPQVTELASMSYKTAFLIGC
                     FQVLSIVPGTSRSGATILGAIIIGTSRSVAADFTFFLAIPTMFGYSGLKAVKYFLDGN
                     VLSLDQSLILLVASLTAFVVSLYVIRFLTDYVKRHDFTIFGKYRIVLGSLLILYWLVV
                     HLF"
     gene            complement(2977..4860)
                     /locus_tag="VBC74_000962"
     CDS             complement(2977..4860)
                     /locus_tag="VBC74_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEO6941925.1"
                     /translation="MKKCFLAICLALSFFMVSVQADEVDYNIPHYEGNLTIHNDNSAD
                     FTEKVTYQFDSSYNGQYVTLGTAGKLPDNFDINNKPQVEVSINGKVRKVSYQIEDLED
                     GYRLKVFNGGEAGDTVKVNVQWKLKNVLFMHKDVGELNWIPISDWDKTLEKVDFWIST
                     DKKVALSRLWGHLGYLKTPPKIRQNNNRYHLTAFNVNKRLEFHGYWDRSYFNLPTNSK
                     NNYKKKIEHQEKIIERHGFILSFLLRILLPSFFIIVTLFISIRVFLFRKKVNKYGQFP
                     KDHHLYEAPEDLSPLELTQSIYSMSFKNFQDEEKKTHLISQEQLIQSILLDLIDRKVL
                     NYDDNLLSLANLDRASDAEIDFIEFAFADSTSLKPDQLFSNYQFSYKETLRELKKQHK
                     ASDLQNQMRRRGSNALSRITRLTRLISKDNINSLRRKGISSPYRKMSSEESKELSRLK
                     RFSYLSPLISFVVIIYTLFLNYFTYFCIYLLLFGVILLLNKIIFMMTRKISNGYIVTE
                     DGASRVYQWTSFRNMLRDIKSFDRSELESIVLWNRILVYATLFGYADRVEKVLRVNQI
                     DIPERFANIDSHRFAISVNQSSNHFSTITEDVSHASNFSVNSGGSSGGFSGGGGGGGG
                     GAF"
     gene            4996..6546
                     /locus_tag="VBC74_000963"
     CDS             4996..6546
                     /locus_tag="VBC74_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO6941926.1"
                     /translation="MKKILLSLFTALLITFGGMTSIQADEYLRVGMEAAYAPFNWTQN
                     DNTNGAVPIEGTDQYANGYDVQVAKKLAKKLNKKVVVVKTKWEGLVPALTSGKLDMII
                     AGMSPTEERKKEINFSKPYYISEPTLVVNAEGKYTNAKNISDFKNAKVTAQQGVYLYN
                     LIDQINGVKKEVAMGDFNQLRQAVESGVVDAYVSERPDATSAQTANPKLKMIELHQGF
                     KTSDADTNISVGMRKGDNRINQVNQVLESISRDKQIALMDKMIKEQPSVKKEKNGKPN
                     FFEQMATILKNNGSQFLRGTATTLLISMVGTIVGLFIGLLIGVFRTAPKSDNKLKAAL
                     QKLLGWLLNIYIEVFRGTPMIVQSMVIYYGTAQAFGVSLDRTLAAIFIVSINTGAYMS
                     EIVRGGIFSVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFILTFTVTRILRYIEKRFDSDNYT
                     TGANQLQV"
     gene            6556..7296
                     /locus_tag="VBC74_000964"
     CDS             6556..7296
                     /locus_tag="VBC74_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6941927.1"
                     /translation="MTQAILEIKHLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STFLRSINLLEEPSGGEILYHGHNVLEKGYDLNNYREKLGMVFQSFNLFENLNILENA
                     IVAQTTVLKRERQEAEKIAKENLNAVGMTEQYWKAKPKQLSGGQKQRVAIARALSVNP
                     EAILFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFAKEVSDRVIFMDKGI
                     IAEQGTPKQLFENPTQERTKEFLQRFLK"
     gene            7638..7868
                     /locus_tag="VBC74_000965"
     CDS             7638..7868
                     /locus_tag="VBC74_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941928.1"
                     /translation="MKLKISVLTAASAVTLLLATSASLAKADTVSYPSRDAGFIYSRG
                     YAPSESSYPSRDAGFIYSRGYAPKHFLSAYDK"
     gene            8236..8352
                     /locus_tag="VBC74_000966"
     CDS             8236..8352
                     /locus_tag="VBC74_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEO6941929.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKHYGKK"
     gene            complement(8444..9328)
                     /locus_tag="VBC74_000967"
     CDS             complement(8444..9328)
                     /locus_tag="VBC74_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEO6941930.1"
                     /translation="MSLIILTVILVLVIVLLITSLYVVKQQTVAIIERFGKYQKTATS
                     GIHIRVPLGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNIATQYRVNENNVTDAYY
                     KLIKPEAQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIVKT
                     LITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVGIA
                     QQRKAIVDGLADSIQELKDANVTLTEEQIMSILLTNQYLDTLNTFAINGNQTIFLPNN
                     PEGVEDIRTQVLSALKTR"
     gene            complement(9476..11140)
                     /locus_tag="VBC74_000968"
     CDS             complement(9476..11140)
                     /locus_tag="VBC74_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEO6941931.1"
                     /translation="MSKITTSLFQEMVQAASTRLSKQAEYVNSLNVFPVPDGDTGTNM
                     GMTMENGAKEVSDKPATTVGEVGQILSKGVLMGARGNSGVITSQLFRGFGQSIKDKEE
                     LTGQDLAHAFQNGVEVAYKAVMKPVEGTILTVSRGAATAALKKAEETDDAVEVMRATL
                     KGAKRALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFKATPATM
                     TEMVNAEHHKAVVGHVATEDIKYGYCTEVMVGLKQGPTYVKEFNYEEFQGYLSNLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVENMRNQHDAQMQKVEVEETVK
                     ETKEYGIIAVVAGDGLAEIFKSQGVDYIISGGQTMNPSTEDIVKAIEKVNARNVIILP
                     NNKNIFMAAQSAADVVDIPAAVVETRTVPQGFTSLLAFDPAKSLETNVADMTNSLSDV
                     ISGSVTLAVRDTTIDGLEIHENDILGMVDGKILVSTPDMEKALKDTFDKMIDEDSEIV
                     TIYVGEDGKQALAETLSEYLEETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            complement(11140..11505)
                     /locus_tag="VBC74_000969"
     CDS             complement(11140..11505)
                     /locus_tag="VBC74_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6941932.1"
                     /translation="MTVKINTRDGQIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQALLRKENYAKGVVVRSSEAGIAVDVYTVMSYGVKISEVSKNIQERVKFNLENQLG
                     LSADMVNVYVQSIKVVGED"
     gene            complement(11637..11825)
                     /locus_tag="VBC74_000970"
     CDS             complement(11637..11825)
                     /locus_tag="VBC74_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEO6941933.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTVLIDGKP
                     KKVWVSARALKSGKVERV"
     gene            12064..12981
                     /locus_tag="VBC74_000971"
     CDS             12064..12981
                     /locus_tag="VBC74_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO6941934.1"
                     /translation="MTRKIGIIGMGHVGSTVAHALIAQGIGDDYVFIDTNVDKVNADM
                     IDFQDALANATYHANIVLNDYDALSDADVVISALGDISLQNNKEANRFAELPFTSKEV
                     YDVSQKLKSSGFRGILIVVTNPVDAITQLYQEYTQFPKSRVIGTGTLLDTARMKRVLG
                     QRLQVDPRSIGGYNLGEHGNSQFVAWSQVTVKGQPAISLLKVEELEQIKEASLKGGHT
                     VFFGKKYTSYGIATAAVRIVLAVLNDSHELLPVSTFYESEGVYLGYPAIIGRVGIVER
                     ISLSLSPKEEQQLTDSAAFIRNYLKHINL"
     gene            complement(13066..13947)
                     /locus_tag="VBC74_000972"
     CDS             complement(13066..13947)
                     /locus_tag="VBC74_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEO6941935.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKLCKQLIETLVESMGITVPVAIHLDHGHYDDALECIEVGYTS
                     IMFDGSHLPVEENLEKAREVVAKAHAKGISVEAEVGTIGGEEDGIVGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPANWEGLDLDHLKKLTEAVPGFPIVLHGGSGIPDDQI
                     QEAIKLGVAKVNVNTECQLAFCQATRDYAVEFNANEAEYMKKKLFDPRKFLKPGFDAI
                     TEAVEERIDVFGSANKA"
     gene            complement(14087..15475)
                     /gene="argH"
                     /locus_tag="VBC74_000973"
     CDS             complement(14087..15475)
                     /gene="argH"
                     /locus_tag="VBC74_000973"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEO6941936.1"
                     /translation="MTKNHKLWGGRFESSLEKWVEEFGASISFDQKLAPYDMKASMAH
                     VTMLGKTDIISQEEAGLIKDGLKILQDKYRAGQLTFSISNEDIHMNIESLLTAEIGEV
                     AGKLHTARSRNDQVATDMHLYLKDKLQEMMKKLLHLRTTLVNLAENHIYTVMPGYTHL
                     QHAQPISFGHHLMAYYNMFTRDTERLEFNMKHTNLSPLGAAALAGTTFPIDRHMTTRL
                     LDFEKPYSNSLDAVSDRDFIIEFLSNASILMMHLSRFCEEIINWCSYEYQFITLSDTF
                     STGSSIMPQKKNPDMAELIRGKTGRVYGNLFSLLTVMKSLPLAYNKDLQEDKEGMFDS
                     VETVSIAIEIMANMLEKMTVNEHIMMTSTETDFSNATELADYLASKGVPFRKAHEIVG
                     KLVLECSKNGSYLQDIPLKYYQEISELIENDIYEILTAKTAVKRRNSLGGTGFDQVKE
                     QILLARKELKAE"
     gene            complement(15494..16684)
                     /locus_tag="VBC74_000974"
     CDS             complement(15494..16684)
                     /locus_tag="VBC74_000974"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEO6941937.1"
                     /translation="MGKEKLILAYSGGLDTSVAIAWLKKDYDVIAVCMDVGEGKDLDF
                     IHDKALTIGAIESYILDVKDEFAEHFVLPALQAHAMYEQKYPLVSALSRPIIAQKLVE
                     MAHQTGATTIAHGCTGKGNDQVRFEVAIAALDPELKVIAPVREWQWHREEEITFAKAN
                     GVPIPADLDNPYSIDQNLWGRANECGVLENPWNQAPEEAFGITKSPEEAPDCAEYIDI
                     TFQNGKPIAINNQEMTLADLILSLNEIAGKHGIGRIDHVENRLVGIKSREIYECPAAM
                     VLLAAHKEIEDLTLVREVSHFKPILENELSNLIYNALWFSPATKAIIAYVKETQKVVN
                     GTTKVKLYKGSAKVVARHSSNSLYDENLATYTAADNFDQDAAVGFIKLWGLPTQVNAQ
                     VNKG"
BASE COUNT         5370 a         3243 c         2640 g         5437 t
ORIGIN      
        1 atgaggtgtg actgctatct aaatgattaa aactaaaaaa gccttttcag gcttttattt
       61 ttgatgctta tcgctatatt tagcaataac actttgtctg taatcactgt tgccaatgaa
      121 ctttaagaga ttaaatagcg gtgtccgatt ctcaccccaa atatttaaac cttctataaa
      181 aatttccatt actaatagta gagccaatag aagaaaaatt cccccaatac gactagaaac
      241 atttaaaagc aaagcgataa gcgaaaagat aattgcaata ccataaacaa ctaaaactgc
      301 tcctctatgt gtaaatccca tagatagcaa tcggtgatgc aggtgcatct tatcagcttc
      361 agaaatcttt ttccctgata atttacgcct cacaatagca acagctgtat ccaaaatagg
      421 aactcctaaa ataatgacag gtgttatcac tgcaacagct gtggagtttt ttaaaccttg
      481 aagcgataat acaccaatca taaatcctat aaacaaggca ccagcatcac ctaggtagat
      541 aatggctgga tgataattat aagggaaaaa accaactatt gaggcgatta aaataacaat
      601 agttagcgtg agaaaaaaat ctattttagg taaaaagaaa taagaaacca ctgccatcgt
      661 tgctagactg atgattgaaa caccactaac tagaccatct agaccatcaa tcagattgat
      721 agcattagtg attgaaataa tccataaaac tgtcaaaaag aaagttaaaa taggaccaaa
      781 ctccaagagt ggaccaccta ttggaatctt aaaactatca aatctaaaat gtgtaaattt
      841 ccatatgata atagctgcaa taataattcc caacatttta taacgaggtc ttaattcaaa
      901 gatgtcatct ataaatcctg ttgttgtaac tacgagaccg ccaataatta caggcaaaat
      961 gtagtgaaaa taagagactt ctataaagtg tctactggcc gccattggca tgaaaaataa
     1021 tgttgttaca acaaaggaga gaaatattgc taaaccacca ctacttggca ttggtacttt
     1081 gttgattctt cgagcgttcg gtttatcaac cgctcctacc ttcaaagata taactcgaat
     1141 aataggagtt agaatgattg ataataaaaa tgctccaatc aatacaaaaa tatactcaat
     1201 tgtaaaaggt atcattttat cccatctcaa tcatttgtaa ttttgaaata gcatcatcat
     1261 gaatcaattg aatactatgc tctttcaagt aagctcgtgt ttttgtgccc acattagcat
     1321 gttcaagcat tcgagcatac atcaaattag caaagtagct tggttggttt tccaagtcta
     1381 gcaaaattgt catatgatag cctttaccat ttttatagag ttcagaagct tctattggaa
     1441 aatcaatcgt ttgtgaaaat cgaactacag cttcaatatt atcaaaatca aagacatagt
     1501 gaacatatcc aatcgtttct acttcctttt caggttgttc ttcagaaaca ttttcctcaa
     1561 ctacttcttg agttgcttta tccatcatat tttcaatttc tgctaatttg gcatgggcat
     1621 ccgtatcccc tttttccaac atcgattgtt ccaaggtttt aaaaaagtct tctggagaca
     1681 tttttgaaat gtcacccaaa tctgctaatt cttctaaatt taaatcttta cttaattcag
     1741 actttgtaac aaaaacatca atgcgatctt ttttaggtgt tactcgaaaa cttaacatac
     1801 cactattttt aaagttttct ggcaagtcta attcatccat gacagaatag aaaaattcct
     1861 cagtcttctc ctgagggatt aggaaatctt tcagctccat accacgatct tctaaatctt
     1921 ccatactaat tgtaattttc agtgttgttt cactgatttg tttcatttcc atatcatcac
     1981 ctcatacgag tattcagttt tcttattata ccgaaaaagc tattttttag caatttttca
     2041 cagcctaaca cgctaaaaag ctgaggaata ctcagctttt tatgttatat tttagaataa
     2101 atgaacaact aaccagtaga ggatgagtaa acttcctaag actatacgat acttaccaaa
     2161 gatggtgaaa tcgtgtcgtt tgacatagtc tgtcaagaaa cgaataacat ataaactaac
     2221 tacgaaagct gtcagacttg ctactaaaag tattaaagat tggtctaaac tcaagacgtt
     2281 accatctaaa aaatatttaa ccgccttaag tccactataa ccaaacatag ttgggatggc
     2341 aaggaagaaa gtaaagtcag cagcgaccga acgactagtt ccaataataa ttgctcctaa
     2401 aatagtagct ccagaacgac tggttcccgg aacaatactg agaacctgga aacatccaat
     2461 caagaaagct gtcttgtaag acatacttgc caattcggtt acctgtggtt tgaggtgtgc
     2521 attacgtttt tcaacccata tgaagacaaa accataaaag attagggcta ttgcaatcgg
     2581 aatcatgaaa ttaaaatgag cttcaaacca attatcaaat ggtaaagcaa ttaaaataga
     2641 tggaatacaa gctatcacta cttttaacca taattgccaa gttaaacgaa tttctctagc
     2701 agatttacct ggctgaaatg ggttaaggcg tttaaaataa atgacaataa ctgccataat
     2761 tgccccaagt tgtataacaa tattaaacat ttcaacaaag gatttgcttt ggttcaactt
     2821 cataaactct tgaaccaaga ttaaatggcc agtactagaa acaggtaacc attctgtcac
     2881 tccctcaacg actcctaaaa atagggcttt gagtaattca attattaaca taataactcc
     2941 ataaattttt ctttattata ccatactttt ggaaaattag aaggcaccgc cacctccgcc
     3001 gcctccgccg cctgagaaac cacctgaaga accgcctgaa ttaacactaa aattagaagc
     3061 gtgactaaca tcttcagtta tcgttgaaaa atgattacta gattgattga ctgaaatcgc
     3121 aaatcgatga ctatcaatgt ttgcaaatct ttctggaata tctatttggt tcactctgag
     3181 tactttctca acacggtcag cgtagccgaa taaagtagcg taaaccaata ttcgattcca
     3241 taatacgata ctttctaact ctgaacgatc aaacgatttg atatccctta gcatgttcct
     3301 aaaactagtc cattggtaga cacgacttgc tccatcttca gttacaatat aaccgttact
     3361 tatttttctt gtcatcataa aaatgatttt attcaacaac aggataacac caaacaataa
     3421 gagatagata cagaaatagg taaaataatt taaaaaaagc gtataaatta taacaacaaa
     3481 agaaataaga ggtgataggt aactgaatct ttttaaccta gataattctt tagactcttc
     3541 tgaagacatt ttacgataag gggatgaaat tccctttctt ctaagagagt ttatattgtc
     3601 tttagaaatc aaccttgtga gacgcgtaat tcttgataag gcattacttc ctcggcgtct
     3661 catttgattt tgcagatctg aagccttgtg ctgctttttc agttcacgta gtgtttcttt
     3721 ataactaaat tggtaattag aaaagagttg atctggcttc aaactcgtag aatccgcaaa
     3781 agcaaactct ataaaatcta tttctgcatc agaagctcta tctaagttag ctagagataa
     3841 caagttatca tcataattca atacttttct atcaatcaag tctaatagaa ttgactgtat
     3901 gagttgttct tgactgataa ggtgagtttt cttctcctca tcttgaaaat ttttaaagct
     3961 catactataa atgctttgag ttaactctaa tggtgaaagg tcctcaggtg cttcatataa
     4021 atgatgatcc ttagggaatt gcccgtattt attaactttt tttctaaaca ggaacaccct
     4081 aattgagatg aatagtgtca caataataaa gaatgaaggt aataatatcc ttaacaagaa
     4141 acttaggata aaaccatgac gctctattat cttctcttga tgttcaattt ttttcttgta
     4201 attattttta ctgtttgtag gtagattaaa ataagatcta tcccaataac catgaaattc
     4261 taatcgtttg tttacattaa aagctgtcaa atggtaacga ttattatttt gtcttatttt
     4321 aggaggagtt ttaagataac ccaagtgccc ccaaagacga gaaagagcaa cctttttgtc
     4381 agttgatatc caaaaatcta ctttctctaa cgttttatcc cagtcgctaa taggaatcca
     4441 gttaagttca ccaacatcct tatgcataaa tagaacattt tttagtttcc actgaacatt
     4501 gactttaaca gtatcacctg cttcaccacc attaaacact ttcaaacggt agccatcctc
     4561 caaatcttct atctggtaac taactttcct tactttacca ttaattgaaa cttcaacctg
     4621 tggcttatta ttaatatcaa aattgtcagg taacttaccc gccgtaccta acgtgacata
     4681 ctgtccatta taggacgaat caaattggta agtaaccttc tctgtaaaat cagcactatt
     4741 atcattgtga atagttagat taccctcata atgaggaatg ttatagtcca cctcatctgc
     4801 ttgaactgaa accataaaaa aactaagagc taaacaaata gccaaaaaac atttcttcat
     4861 tatcatcctc cctcctttgt ttgtatacca ttttaacata aaaatacaat taatcgcttt
     4921 caataagata atgatattga aatcgacctt gtttttcggt aaaataggag gaagtaactt
     4981 atcggaggct gttgaatgaa aaaaatatta ctaagtttat tcactgcttt gcttatcacc
     5041 tttggaggta tgacaagtat acaagcagat gaatatctac gtgtcggtat ggaggcagcc
     5101 tatgcgcctt tcaactggac acaaaatgat aacacaaatg gtgcagttcc tattgaagga
     5161 actgatcaat atgctaatgg ttatgatgta caagtcgcta aaaagttagc taaaaagtta
     5221 aataaaaaag ttgttgttgt caaaacaaaa tgggaaggcc ttgttccagc actaacttct
     5281 ggaaaactcg atatgattat tgcaggaatg agtcctaccg aagaacgtaa aaaagaaatt
     5341 aatttttcaa agccttacta catttcagaa cctactctag ttgttaacgc tgaaggaaaa
     5401 tatactaatg ctaaaaatat tagtgatttc aaaaatgcta aagtaactgc ccaacaagga
     5461 gtctacttat acaacctcat tgatcaaatc aatggtgtca aaaaagaagt tgccatgggg
     5521 gactttaatc aattaagaca agctgtagaa tctggtgttg tagacgccta tgtttcagaa
     5581 cgtccagatg caacttccgc tcaaactgct aatcctaaat taaaaatgat tgaactgcac
     5641 caagggttta aaacatctga tgccgataca aatatttccg ttgggatgcg taaaggtgac
     5701 aatcgtatta atcaagtcaa tcaagttctc gaaagcattt ctagagataa acaaatcgcc
     5761 ttgatggata agatgattaa agaacaacca tccgttaaaa aagaaaaaaa tggaaaacca
     5821 aacttctttg aacagatggc aactatttta aaaaacaatg gttctcaatt cctcagagga
     5881 acagctacaa ccctcctaat ttctatggta ggaactatcg tcggattatt tatcggcctt
     5941 ctcatcggtg ttttccgtac agcaccaaaa tctgacaata agttaaaggc tgccttacaa
     6001 aaattattag gttggttact taacatctat attgaggtct tccgcgggac acctatgatt
     6061 gtacaatcaa tggttatcta ctacggaaca gcacaagctt tcggagtttc gctggaccgt
     6121 acactagcag ctatatttat tgtttcaatc aatactggtg cttatatgag cgaaattgta
     6181 cgtggtggta ttttctctgt tgataaagga caatttgaag ccgcaactgc acttggtttc
     6241 acccatggac aaaccatgcg aaaaattgtt ctaccacaag ttgtacgtaa catactccca
     6301 gctactggta atgagtttgt tatcaatatc aaagatacct ctgtactgaa cgttatctct
     6361 gttgtcgaac tctacttctc tggtaatact gttgcaacac aaacctacca atatttccaa
     6421 acattcacca ttattgcgat tatttacttc atcttaacct tcaccgtaac acgtatctta
     6481 cgttacattg aaaaacgttt tgattcagat aattacacaa ccggtgcgaa tcaattacaa
     6541 gtctaggagg ttggtatgac acaagctatt ttagaaatta aacatttgaa aaaatcttac
     6601 ggaagcaatg aagtgttaaa agacatttcg ctttcggtca acaaaggcga agttatttct
     6661 atcattggtt cttctggatc aggaaaatca actttcctac gttctattaa cctcttagaa
     6721 gaaccaagtg gaggagaaat cttatatcac ggtcacaatg ttcttgaaaa aggctatgat
     6781 ttaaataatt atcgtgaaaa acttgggatg gtctttcaat cttttaacct ctttgagaat
     6841 ttaaacatct tagagaacgc tattgttgca cagactactg ttctaaaacg tgaacgccaa
     6901 gaagctgaaa agattgctaa agaaaactta aatgctgttg gcatgactga gcaatattgg
     6961 aaagctaaac caaaacaatt atcaggtggg caaaagcaac gtgtcgcaat cgcccgagca
     7021 ctttctgtta acccagaagc tatacttttt gatgaaccaa cttcagcctt ggatccagaa
     7081 atggttggtg aggtccttaa aacaatgcaa gatctagcta aatctggatt aacaatgata
     7141 attgtgacac acgagatgga atttgctaaa gaagtatcag accgcgttat tttcatggac
     7201 aaaggaatca ttgcagagca gggtactcct aagcaacttt tcgaaaatcc aacacaagaa
     7261 agaactaagg aatttctaca acgcttctta aaataaaatt tatccacatt taaatataga
     7321 ctatttatac ccctacaggt tttgtcattg tcatcattac aatggaactg gaggggattt
     7381 tttattacca catctttgat gaaatctacg actagcctca actatagatc aaattagaca
     7441 gtcgttattg gattgtatac tattaaataa gttactaaat ctcatttttt aattttaatc
     7501 gatatttaat taattatata tttgtttaaa attaaaaaca tgttgtttta atgagctttt
     7561 gatgatataa tatagtatgc acaaaatgaa ctaaccactg tgcaagaata gtttattatc
     7621 tttgaaagga ttttataatg aaacttaaaa tcagtgtttt aacagcagct tcagctgtca
     7681 ctctattatt agctacttca gcaagtcttg cgaaggcaga cacagtgtct tatccttcac
     7741 gtgatgccgg atttatctac tctcgtgggt atgcgccttc tgaatcaagc tatccttcac
     7801 gcgatgccgg gtttatctac tctcgtggat acgctccaaa acacttccta agtgcttacg
     7861 ataaataaaa actgacctta ttaacttaac cgatacactt tcacaagtat aaattttcat
     7921 tcaattacat taaaaataat tatttagttt gctgaaaaac gatcactcat cgatcgtttt
     7981 tttatatgca caatgatagc cattagctac tttctcgttg ggtattttcc tcattcatct
     8041 ttcacttaac atcacttgtc aaatctaaac ctcattaata aaggtttaat atatctttga
     8101 acttattact tttgattgat atttaattac tttgctattt atttttaaat ttatcaacaa
     8161 agtactttac cagttaattt ttttgtggta tactggtgtc tgttaacgtt aataaattgt
     8221 ctataggagg taatcatggc tgaaaaaaca gacctatcgt cagcctatcg cagacttaaa
     8281 agtcctaaca ttaaaactag aaaacgtgct ctgaaaatca ttcatgagta caaacattac
     8341 ggtaaaaaat aacttttttc cgtctctcca attataataa ataagtcaga cacaaaataa
     8401 ccagagcaaa tgctctggtt attttgcttc tataaaaagt tcattatctt gttttaagag
     8461 cagataaaac ttgtgtacga atatcttcaa caccttctgg gttatttggt aagaaaatcg
     8521 tttgattacc attaatagca aatgtattta aagtatctaa atattgattt gttaacaaga
     8581 tagacatgat ttgttcttct gtcaatgtca cgttagcatc tttcaattct tgaatcgaat
     8641 cagctaaccc atctacaatt gctttacgtt gttgcgcaat acccacacca tgtagacgat
     8701 ctttttctgc ttctgcttct gcagcagtta caattttgat cttatcagca ttcgctaatt
     8761 cttgagctgc tacacgttta cgttgtgcag cattgatttc gttcatggat tgttttactt
     8821 ctgcatctgg ctcaacctta gtaattaaag tcttaacaat gatgtagccg tatgttgaca
     8881 tttcttctgc cacttgatgt tgaacttcca aggctatttc atctttcttt tcgaatagct
     8941 catctaatgt aagtttagga actgatgaac gcaaagcatc ttcgatataa gatttaatct
     9001 gagcttctgg ctttattaat ttgtaataag cgtctgtaac gttattttca ttgacacgat
     9061 attgtgtagc aatatttaaa gtgacaaaaa cattatcttt tgtttttgtc tcaactataa
     9121 tttcactctg taaaagacgt aactgcactc gtgctgcaat cttatcgata cccaatggta
     9181 cacgaatatg aataccgcta gtagcagtct tttgatattt accaaagcgt tcaatgatgg
     9241 ctaccgtctg ttgtttaaca acgtataaag aagttatcaa cagaacaatc actaaaacca
     9301 aaataaccgt aagaataatt aatgacatca taaatctcct ttgtatgaat gttttaaaac
     9361 aattctagca caatttgtca aaattttcaa aatactgatg ctttatcata ctaaaataga
     9421 aactctgata aaataaaaaa ttagaagtat ctctcctaat tttttatttt atcaattatt
     9481 caacgctcat gaggtatgga tatacaggtt gatctccttg gtggatctca acttcaacat
     9541 cttcataagt ctcctcaaga tattccgaca aggtctcggc taatgcttgt ttaccatctt
     9601 caccaacata gattgtcacg atttcactat cttcatcaat cattttatca aaagtgtctt
     9661 taagtgcttt ttccatatct ggtgtagata ctagaatttt accatcaacc atcccaagga
     9721 tgtcgttttc atgaatttca aggccatcta tcgttgtatc acgaacagct aaagtcacac
     9781 taccactaat gacatctgag aggctatttg tcatatcagc tacattagtt tcaagagact
     9841 tagcaggatc aaaagcaagt aagctagtaa atccttgtgg aactgtacgt gtctctacta
     9901 ctgctgctgg aatatcaacg acatctgcag ctgactgtgc agccatgaag atgttcttat
     9961 tgtttggtaa aataataaca tttctagcgt ttactttttc aatcgctttt acaatatctt
    10021 ctgtagatgg gttcatagtc tggccgccag agatgatata atcaacacct tgtgatttaa
    10081 aaatctcagc tagaccgtca ccagctacaa cagcaataat accatattct ttagtttctt
    10141 tgacagtttc ttcaacttca accttttgca tttgggcatc atgttggtta cgcatgtttt
    10201 caactttaac cttcacaagc gatccatatt tcaaaccttc ttgcataaca agacctggat
    10261 cttctgtatg aacatgaacc ttgacaattt catcatcatt aacaactagt aaagaatccc
    10321 ctagattgct aagataacct tgaaattctt cgtagttaaa ttccttaacg taagttggtc
    10381 cttgctttaa accaaccatg acttctgtac aatagccata tttaatatct tctgtggcta
    10441 cgtggcctac tacagcttta tggtgctctg cattaaccat ttcagtcatt gttgcgggag
    10501 ttgctttaaa atcttctgat gcgatatatt ctccagtcag tgctgagagg aaaccttcgt
    10561 aaataaagac gagtccttga ccaccagaat caacaacacc aacctcttta agaacaggta
    10621 gcatatccgg tgttttagca agagcacgct tagcaccttt gagagttgcg cgcataactt
    10681 caactgcatc atcagtttct tcagcttttt ttagagcagc ggtagctgca ccgcgtgata
    10741 ctgtcaagat agttccttca actggtttca taacggcttt ataagctact tcaacaccat
    10801 tctggaaagc gtgtgccaaa tcttgacccg ttaattcttc tttatccttg atactttgac
    10861 caaaaccacg gaataattgt gatgtaatta cacctgaatt tccacgtgct cccatcaata
    10921 cacctttaga aagaatttga ccaacttctc caacagtcgt cgctggtttg tccgaaactt
    10981 ccttggctcc gttttccata gtcatcccca tattcgtacc agtatcacca tctggaactg
    11041 ggaatacatt taatgagttg acatactctg cctgtttact cagacgtgta ctggcagctt
    11101 gtaccatttc ttggaataaa cttgtcgtaa tctttgacac taatcttctc ctacaacctt
    11161 aatactttgc acataaacat tcaccatatc agcagataaa ccaagctgat tttcgagatt
    11221 gaatttcact cgctcttgaa tattttttga aacttcacta attttgacgc cgtaactcat
    11281 gacagtataa acatctactg cgatgcccgc ttcactagac ctcacaacaa caccttttgc
    11341 gtagttttcc ttacgcaaaa gtgcttgaaa gttatctttg atagcacttt tacttgccat
    11401 accaacaacg ccaaaaattt ccgttgctga acctccaacg acagttgcaa taacgtcatc
    11461 agagagttca atttgaccat ctcttgtatt gatttttaca gtcatatcta ctacctcaaa
    11521 aagtttttat cactctattc tatcatatct cactattatt gtaaaaaaat agaggtatct
    11581 aactatcaaa tcagccttag aaacactaaa aaaagcccta aggctttctc agtttattaa
    11641 acgcgttcta ctttaccaga tttaagcgca cgagctgaaa cccaaacttt ttttggttta
    11701 ccgtcgataa gaacagtaac tttttgaagg tttggtttaa cagtacgttt tgtttggttc
    11761 atcgcgtgtg aacggttgtt accagaaact gttttacgtc ctgtgaaata acatacttta
    11821 gccattttac gagttcctcc tatattgatc aatatttacg gatgtgctag caccacatac
    11881 tagactattc tatcacaact tacttcttct agcaagttct tttcaaaatt actttttcat
    11941 ttgttttatc taaatcgctt acatattttt tataataaga cacacttcct tctaattcat
    12001 tttttagttt acattttctt tcaaaatatt ataatagaca acaataatca aaggaggttt
    12061 catatgacac gtaaaattgg aattataggc atggggcacg ttggatcaac tgttgcacac
    12121 gctcttattg cacaaggtat tggtgatgat tatgtcttta ttgatactaa tgttgataag
    12181 gttaatgctg atatgattga ttttcaagat gctctggcaa atgctactta tcacgctaat
    12241 atagtattaa acgactatga cgcgttgtca gatgctgatg ttgtgatttc agccttaggt
    12301 gatatttcac tacagaataa caaagaagct aatcgctttg ctgaactacc atttaccagt
    12361 aaagaagtct acgacgttag ccaaaagtta aaatcatctg gttttagagg gatccttatt
    12421 gtcgttacta atcctgttga tgccatcacc cagctttatc aagaatatac tcaatttcct
    12481 aaatcacgcg ttatcggaac aggaacctta ttggatactg ctcggatgaa aagagtgctt
    12541 ggtcaacgcc tacaagttga tccaaggagt atcggtggtt ataatctagg agagcatggt
    12601 aactcccaat ttgtcgcttg gagtcaggta actgtaaaag gacaaccagc tatatcacta
    12661 ttaaaagtag aagaattaga acagataaaa gaagcttctt taaaaggcgg gcacactgtc
    12721 ttttttggta aaaaatatac tagttatggt atcgctaccg cagctgtccg catcgtttta
    12781 gctgtgttaa atgattccca tgagttacta cctgtatcta ccttctatga gtccgaaggg
    12841 gtttaccttg gatatccagc tattattggt agagtaggaa ttgtagaaag aatttctctt
    12901 tcactaagcc caaaagaaga gcaacaactc acagattcag cggcatttat tcgaaattat
    12961 cttaagcata tcaatcttta aagagtatct atttaataaa aagtcatata atccaaaaaa
    13021 agctcaccat aagtgagctt ttaactatta tctgttttat gttaattatg ctttgtttgc
    13081 tgaaccaaac acgtcaatac gttcttcaac tgcttctgta attgcatcga aaccaggttt
    13141 caagaattta cgtgggtcaa agagtttttt cttcatgtat tcagcttcgt tagcgttgaa
    13201 ttcaacagcg tagtcacgag ttgcttgaca gaaagctaat tgacattctg tgttaacgtt
    13261 aactttagca acaccaagtt tgatagcttc ttggatttga tcatcaggaa tacctgaacc
    13321 accgtgtaac acgattggga aaccaggaac agcttcagtc aatttcttca agtgatcaag
    13381 gtcaagtcct tcccagtttg ctgggtatgg tccatgaatg ttaccgatac ctgcagccaa
    13441 gaagtcaata ccagtttcaa ccatagcttt agcgtcttcg attggtgcta attcaccttt
    13501 accgacaata ccatcttctt caccaccgat agtaccaact tcagcttcta ctgaaatacc
    13561 tttagcatgt gctttagcaa caacttcgcg tgctttttca aggttttctt caactggaag
    13621 gtgtgaacca tcaaacataa ttgaagtgta acctacttca atacactcaa gggcatcatc
    13681 gtaatgaccg tggtcaaggt ggatagcaac tggtacagta atacccattg attcaaccaa
    13741 agtttcaata agttgtttac aaagtttgta accacccatg tatttagccg cacccattga
    13801 agtttggata agtactggag cttttttagc ttctgctgcg cgcaagatag cttgagtcca
    13861 ctcaaggttg tttgtgttaa atccacctac tgcatagccg ttatcacgag ctgcttggac
    13921 gaatttttct gctgaaacga ttgccattat tcaggcctcc tatttatttt ttgggcgttg
    13981 cccacttaca tgttaattct agcacaattc tataaaaaat gctagttttt tactgaaaat
    14041 acgtgagaaa cttaacagag ttaaacgagg ttttttcaga aagcagtcat tccgctttca
    14101 attctttacg tgccaacagg atctgttctt taacctggtc aaaccctgtt cctcctaatg
    14161 aatttcttcg tttaactgct gtctttgcag ttaatatctc atagatatcg ttttctatca
    14221 actctgaaat ttcttgataa tattttaaag gtatatcctg taaataagaa ccatttttac
    14281 tgcattctaa aaccaattta cctacaattt catgtgcttt tctgaaaggt acccccttac
    14341 ttgctaaata atctgctaat tctgttgcat ttgaaaaatc agtctccgtt gaggtcatca
    14401 ttatgtgctc attcacagtc attttctcaa gcatattagc catgatttca atagctatgc
    14461 tgactgtttc tacgctatca aacatccctt ctttatcttc ttgtaaatct ttgttataag
    14521 ctaacggcaa tgatttcata acagttagta aactaaataa atttccataa acacggcctg
    14581 tcttccctcg aattaattct gccatatcag gatttttctt ttgaggcata atagatgatc
    14641 ctgtagagaa agtatctgat aatgtgatga attgatattc ataactgcac caattaatga
    14701 tttcttcaca aaaacgtgat aaatgcatca ttaaaatgct agcattggat aaaaattcta
    14761 tgataaagtc acgatctgag acagcatcaa gcgaattact atatggtttt tcaaaatcta
    14821 ataaacgagt tgtcatatgg cggtcaatag gaaatgttgt ccctgctaga gctgctgctc
    14881 ctaatggtga tagattggtg tgtttcatat taaactctaa tcgctctgta tcacgcgtaa
    14941 acatattata gtaagccatt agatgatgtc caaatgaaat cggctgagca tgttgtaaat
    15001 gtgtatatcc tggcataaca gtatagatat gattttctgc taaattaact aaggttgttc
    15061 gtaaatgaag caatttcttc atcatttctt gcaatttatc tttcaaataa aggtgcatat
    15121 ctgtagccac ttgatcattt cgtgaacgtg cagtatgaag cttacctgct acttctccaa
    15181 tttcagcggt taataagctc tctatgttca tatgaatgtc ttcatttgat atgctaaaag
    15241 tcaattgacc tgctctgtat ttatcttgca gtatttttaa accatctttt atcaagcctg
    15301 cttcttcttg actaataata tccgttttgc ctaacattgt gacatgggcc atagaagcct
    15361 tcatatcata gggagctaac ttttggtcga aactgattga tgcaccgaac tcttcaaccc
    15421 atttttcaag actactttca aaacgaccac cccataactt atgatttttt gtcatcctct
    15481 tatcttcctt tatttatcct ttgtttactt gtgcattaac ttgggttgga agtccccaga
    15541 gtttgataaa accaactgct gcatcttgat caaagttatc agcagctgta taagttgcta
    15601 agttctcatc atataatgaa ttagacgagt ggcgagcaac gactttggca gaacctttat
    15661 ataacttaac ttttgtagta ccattaacca ctttttgtgt ttctttgaca taggcaatga
    15721 tagcttttgt agctggacta aaccataaag cattataaat aagatttgat agttcatttt
    15781 caaggatagg tttaaaatga gacacttcac ggactaatgt taaatcttca atttctttat
    15841 gagcagctaa cagtaccatt gccgcaggac attcataaat ttcacgtgat ttgataccga
    15901 ctaaacgatt ttcaacatga tcaatgcgtc caataccatg ttttcctgca atttcattta
    15961 gcgataaaat caaatctgct agtgtcattt cttgattgtt tatagctata ggcttcccat
    16021 tttggaacgt aatatcaata tattctgcac aatcaggagc ttcttctggt gattttgtaa
    16081 taccaaaagc ttcttctggc gcttgattcc aaggattctc aagcacacca cattcattgg
    16141 cacgtcccca taaattttgg tctatagaat aagggttatc taaatctgct ggaataggta
    16201 cgccgttagc ttttgcaaaa gtaatttcct cttcacgatg ccactgccac tcacgaacag
    16261 gggcaattac ttttaattca ggatctagtg ctgcaattgc aacttcaaaa cgcacttggt
    16321 catttccttt acctgtacat ccatgtgcaa ttgtggtcgc acctgtttga tgtgccattt
    16381 caactaattt ttgggcaata attggacgac taagcgctga aacaagcggg tatttttgtt
    16441 catacatcgc atgtgcctgt aaagctggta aaacaaaatg ctcagcgaac tcatctttaa
    16501 catctaaaat ataagattct atggcaccta ttgttaaagc tttatcgtga ataaaatcaa
    16561 gatctttacc ttcgccaaca tccatacaga cagcaataac gtcataatct ttttttaacc
    16621 aagctatagc aacagaggta tctaaaccac cagaataagc taaaattaat ttttcttttc
    16681 ccataaaaaa
//
LOCUS       DATROD010000031        16604 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.031, whole genome shotgun sequence.
ACCESSION   DATROD010000031 DATROD010000000
VERSION     DATROD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16604
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.031"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            123..1742
                     /gene="nikA"
                     /locus_tag="VBC74_000975"
                     /pseudo
     CDS             123..1742
                     /gene="nikA"
                     /locus_tag="VBC74_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
     gene            1729..2673
                     /locus_tag="VBC74_000976"
     CDS             1729..2673
                     /locus_tag="VBC74_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6941938.1"
                     /translation="MYLSSSIIKKILSAFLALFFISLLTFILIKLSTVNPAENYLRLS
                     KISVSPEALKEAEHYLGLDKPLWKQYWLWFQKALTGDFGYSYVLRLPVLDLVLQRFLA
                     TLFLGTSAFLLIVTISTPLGVWAGLHESARSDHLIRFLSFSSVSMPNFWVAYLLMLLF
                     SAKLNLLPVSGGNDLQSLILPSITLSFSTVGQYIALIRKAISQENRSLNVENARLRGV
                     KERYIVTHHLLRNALPAIMTALSLTWVYLLTGSIIVEEIFSWNGIGRLFVTSLRTSDL
                     PVIQACMLIFGTLFLANNFMTQCFMNWVDPRLRKSREK"
     gene            2673..3479
                     /locus_tag="VBC74_000977"
     CDS             2673..3479
                     /locus_tag="VBC74_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6941939.1"
                     /translation="MSKRIYFSSSLLVLLVISAIFAPILSSFDPQYVDLSQKLLAPNN
                     VHLLGTDQLGRDVLSRLLYGARYSLFLAIIISLLELTIGMFVGLIVGWYQGKLENLFL
                     WIANIILAFPSFLLSLATVGILGHGLGNLIFAIVFVEWVYYAKLMTNLVKSAKKEPYV
                     INAQIMGLSVWHILRKHIFPFVYQPILVMVLMNIGNIILMISGFSFLGIGVQPNVTEW
                     GMMLHDARGYFRTATWMMLSPGIAIFLTVFSFNTLGDAIDKKDWKRQWNS"
     gene            3467..4255
                     /locus_tag="VBC74_000978"
     CDS             3467..4255
                     /locus_tag="VBC74_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6941940.1"
                     /translation="MEQLEIRKLSLQIGEVPVLRDFSCKIDMGESLTIIGESGSGKTL
                     LAKLLVGHIPQGMTVRGNIFFKGVDLGKLTVKQWQKLRGRDIAYLVQNPMSMFNPFQK
                     IEAHILETILSHEKCSKRVALSKALEWMKRLNLDDAISLLKKYPFELSGGMLQRIMLA
                     TILSLDPQVIILDEPTSAVDCHNCSTISAILQELQNNGKTLITVTHDYQLARDLGGQL
                     LVISEGEVVEQGQTQAILSNPQHNYTKALTVQMEYEGDILNVGL"
     gene            4242..4922
                     /locus_tag="VBC74_000979"
     CDS             4242..4922
                     /locus_tag="VBC74_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6941941.1"
                     /translation="MLVCNHVGKTFGRQEVLKDCHFHLKRGEIIGIMGKSGSGKSSLA
                     RLIIGLDSPTCGSIYFQGKIYTPKDGKAQIILVFQDALSSVNPYFSIEEILNEAFYGK
                     KTTFELCQILEAVGLDGTYLKYKARQLSGGQLQRVCIARALLLKPKIIIFDESLSGLD
                     PVTQIKMLRLLQKIKRRYELSFIMISHDPKICQAICNRVFLIKNGYLVEDNEFLKRAC
                     STNCLTNL"
     gene            5043..5771
                     /locus_tag="VBC74_000980"
     CDS             5043..5771
                     /locus_tag="VBC74_000980"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEO6941942.1"
                     /translation="MEPKYQRILIKLSGEALAGDKGVGIDIPTVQSIAKEIAEVHNSG
                     VQIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQYGVDTRVQT
                     AIPMQTVAEPYVRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEAEAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHVEVIKRGLKIMDATASTISMDNDIDLVVFNMN
                     ETGNIKRVVLGEQIGTTVSNKASE"
     gene            5787..6344
                     /gene="frr"
                     /locus_tag="VBC74_000981"
     CDS             5787..6344
                     /gene="frr"
                     /locus_tag="VBC74_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEO6941943.1"
                     /translation="MTKEIVTKAQERFEQSHQSLSREFAGIRAGRANASLLDRIQVEY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERAINESDLGINPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKIAIRNIRRDAMDEAKKQEKNKEITEDDLKSLEKDI
                     QKATDDAVKHIDEMTANKEKELLEV"
     gene            6462..7316
                     /gene="cvfB"
                     /locus_tag="VBC74_000982"
     CDS             6462..7316
                     /gene="cvfB"
                     /locus_tag="VBC74_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEO6941944.1"
                     /translation="MNTLLATVITGLVTDENKDFYFIQKDGFTFALSKSEGEHHIGEM
                     VKGFAYTDMQQKARLTTKETFATRDHYGWGTVTEVRKDLGVFLDTGLPDKQVVVSLDV
                     LPELKELWPKKGDRLYVCLDVDKKDRLWALPADPEVFQRMATPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYSEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKKIKQ
                     DQLGTELL"
     gene            7454..7963
                     /gene="msrA"
                     /locus_tag="VBC74_000983"
     CDS             7454..7963
                     /gene="msrA"
                     /locus_tag="VBC74_000983"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEO6941945.1"
                     /translation="MERAIFAGGCFWCMVQPFEELDGIESVLSGYTGGHVENPTYKEV
                     CSKTTGHTEAVEIIFNPEKISYADLVELYWAQTDPTDAFGQFEDRGDNYRPVIFYENE
                     EQRQIAQKSKDKLQASGRFDRPIVTSIEPADTFYPAEDYHQAFYRTNPARYALSSARR
                     HAFLEENWH"
     gene            7960..8175
                     /locus_tag="VBC74_000984"
     CDS             7960..8175
                     /locus_tag="VBC74_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEO6941946.1"
                     /translation="MRKSFYSWLMTQRNPKSNEPVAILADYAFDETTFPKHSSDFETV
                     SRYLEDEASFSFNLTDFDDIWEDYLNH"
     gene            8332..10104
                     /locus_tag="VBC74_000985"
     CDS             8332..10104
                     /locus_tag="VBC74_000985"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEO6941947.1"
                     /translation="MRYTNGNFEAFARPRKPEGVDKKSAYIVGSGLAGLAAAVFLIRD
                     GQMDGQRIHIFEELPLSGGSLDGVKRPDIGFVTRGGREMENHFECMWDMYRSIPSLEV
                     PDASYLDEFYWLDKDDPNSSNCRLIHKQGNRLESDGDFTLGTHSKELVKLVMETEESL
                     GAKTIEEVFSKEFFESNFWTYWATMFAFEKWHSAIEMRRYAMRFIHHIGGLPDFTSLK
                     FNKYNQYDSMVKPIISYLESHNVDVQFDSKVTNISVDFKNGQKLAKAIHLTVGGEAKT
                     IDLTPNDFVFVTNGSITESTNYGSHDTVAKPNTDLGGSWNLWENLAAQSDEFGHPKVF
                     YKDIPKESWFVSATATIKDPAIEPYIERLTHRDLHDGKVNTGGIVTVTDSNWMMSFAI
                     HRQPHFKEQKENETIVWIYGLYSNVEGNYIKKPIEECTGREITEEWLYHLGVPEMKIH
                     DLSDKQYVSTVPVYMPYITSYFMPRVKGDRPDVIPQGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYTFLNIERGVPEVFNSAFDIRVLLQSLYYLNDKKSVEDMDLPIPAL
                     MRKVGMKKIRGTYLEELLREAHLL"
     gene            10228..11214
                     /locus_tag="VBC74_000986"
     CDS             10228..11214
                     /locus_tag="VBC74_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEO6941948.1"
                     /translation="MQEYSIEITLQHPDDMMSLFGSNERHLKLIEENLDVIIHARTER
                     VQVLGDSEEAVETARLTIEALLVLVNRGMTVNTSDVVTALSMAQNGSIDKFVALYEEE
                     IIKDSYGKPIRVKTLGQKIYVDSVKNHDVVFGIGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKEQTSRLMEREIIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDVSQIDLPKNVKS
                     GLIDAVEKLRNIKKIDFIHLSAKDVVRHPVVAEIINAYSDSESSHKLQSDKD"
     gene            complement(11305..12108)
                     /locus_tag="VBC74_000987"
     CDS             complement(11305..12108)
                     /locus_tag="VBC74_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4037 domain-containing protein"
                     /protein_id="HEO6941949.1"
                     /translation="MDHFTKLWQDFSKLPNVVAIALGGSRSGDSFDQSSDYDLYVYCA
                     ATPDITSRKRILNKHCHYIELNNHYWELEDNGTLNDGTDIDILYRNIDNFLSDLEDVV
                     EHHNSRIGYTTCFWHNLINCQILYDPENQLQSLKERFEVSYPSQLQKQIIIQNRNLLT
                     GKLPSYDKQIIKALKRQDFVSTHHRTTAFLDSYFDIIFALNKLTHPGEKRMISYAKKN
                     ATLLPKHFEENIIKLCHHNSNEHTVKETLNDIIMHLDVMLKENFQHFIG"
     gene            12300..12776
                     /locus_tag="VBC74_000988"
     CDS             12300..12776
                     /locus_tag="VBC74_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO6941950.1"
                     /translation="MKQDYISYIRSKVGHETIFLTYSGGILTDGKGRVLLQLRADKNS
                     WGIIGGRMELGESSVDTLKREFFEETGLRVEPIRLLNVYTNFQDSYPNGDKAQTVGFI
                     YEVSCPKPVNIEGFHNEETLQLDYFSKEDVKNITIVNEQHQLILDEYFSQTFQMGR"
     gene            13098..13904
                     /locus_tag="VBC74_000989"
     CDS             13098..13904
                     /locus_tag="VBC74_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6941951.1"
                     /translation="MKITLHGVAETLLITLYIRAKDAMAKHPILNDQKSLAIVEQIEY
                     DFDKFDNSEASFYATLARIRVMDREIKKFIRENPNSQILSIGCGLDTRFERVDNGQIR
                     WYNLDLPEVMEIRKLFFEEHERVTNIAKSALDETWTREVNPQNAPFLIVSEGVLMFLK
                     EDDVETFLHILTNSFSQFMAQFDLCHKEMINKGKQHDTVKYMDTEFQFGITDGHEIVD
                     LDPKLKQINLINFTDEMSKFELGTLRSLLPTIRKFNNCLGVYEYKASEKK"
     gene            14299..14796
                     /gene="ybeY"
                     /locus_tag="VBC74_000990"
     CDS             14299..14796
                     /gene="ybeY"
                     /locus_tag="VBC74_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEO6941952.1"
                     /translation="MYVEMIDETGQVSEDIKKQTLDLLEFAAQKTGKENKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPEVDISFDEEDLAENPELAEMLEDFDSYIGEL
                     FISIDKAKEQAEEYGHSYEREMGFLAVHGFLHINGYDHYTPEEEKEMFSLQEEILTAY
                     GLKRQ"
     gene            14777..15175
                     /locus_tag="VBC74_000991"
     CDS             14777..15175
                     /locus_tag="VBC74_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEO6941953.1"
                     /translation="MDLNDNNQKKWKNRTLTSSMEFAVTGIFTAFKEERNMRKHLVSA
                     ILVILAGLTFQVSMVEWLFLLLSIFLVITFEIINSAIENVVDLASNYHFSMLAKNAKD
                     MAAGAVLVVSLFAVLVGLIIFIPKILALLL"
     gene            15217..16116
                     /gene="era"
                     /locus_tag="VBC74_000992"
     CDS             15217..16116
                     /gene="era"
                     /locus_tag="VBC74_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEO6941954.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGD
                     DMIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALQGNNVPT
                     LIKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
     gene            16353..>16604
                     /locus_tag="VBC74_000993"
     CDS             16353..>16604
                     /locus_tag="VBC74_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941955.1"
                     /translation="MHNILRFLGIVIISAVILFSIGSFYDLSLMKNILLICWSFLFDL
                     LVFVFKQRQTTEVLTWYQVVKQFWLFIKCTILIPILVAFI"
BASE COUNT         5509 a         2415 c         3307 g         5373 t
ORIGIN      
        1 aagtaaattt tgtatcgttg tagatttttt attttaagat tcttgacaag acattactaa
       61 aacgatttaa ttaagtttgc ttaaaaaaat aattaactaa acttaaaaga aagttggtaa
      121 aaatgagacg taacatatta ttgagcatta cttgtttatt gatggtgact ctgactgcat
      181 gccattcaca ggatagcaaa agtcataaac taaacagtga taaactcacg cttgcttggg
      241 gagaagactt tggtgatgtt aatccgcatc gctataaccc tgatcagttt gttatccagg
      301 atatggttta tgaggggttg gtacgatatg gtgacaatgg gaaaattgaa ccagctttag
      361 cgaaaagctg gagcattagt caagatggaa aaacctacac ttttaaattg agaaatgcta
      421 aatattctga tggcagtaac tttaatgctg cgaatgtcaa acgtaatttt gatagtattt
      481 tttcgaagtc taatagaggt aatcataatt ggtttaattt aacgaatcaa ttggaaaatt
      541 atcgtgcctt gaatcaaagt acttttgaaa ttaaactgaa acaagcttat agtgctactt
      601 tatatgatct ctctatgatt cgaccaattc gctttttatc tgatagcgct ttccctaaag
      661 gtgatgatac aactaagaag aatgtaaaaa aaccaattgg aacgggtcaa tgggttgtga
      721 aaagtaaaaa gcaaaatgaa tatattacct tcaaacgtaa tgagaattac tggggaaaga
      781 aaccaaaatt aaaagaagta accgtaaaag ttatacccga tgctcagacg cgtgctttag
      841 cattcgaatc aggtgacgtt gatttgatct atgggaatgg cattattggt ttagacactt
      901 ttgcccaata tacgaaggac aagaaatatg tcacagctat ttctcaacct atgtcaacaa
      961 gacttttgtt gctaaatgct aaggaaagta ttttccaaga taaaaaagtt cgtcaggcaa
     1021 tgaatcatgc tattgacaag gtttctattg ctaagaacac ctttagagga acagaaaagc
     1081 cagcagacac tattttttca aaatcaacat ctcactcaga tgctaagtta aatccttata
     1141 gctgatttgc ttttgataaa gcaaatcagc tattggatca agctggttgg aagatgggaa
     1201 aagataaggt tcgtgaaaaa gatggtaaag ctctaacctt acgtcttcct tatatcgcca
     1261 ctaaggcaac tgataaagac ctggtaactt atttccaagg agaatggcgt aaaatcggta
     1321 ttaatgtttc tcttattgct atggaagaag atgattattg ggctaatgct aaaaagggta
     1381 attttgatat gatgttaacc tattcttggg gagcaccatg ggaccctcat gcttggatgt
     1441 cggcattaac tgccaaggca gatcatggac atccagaaaa tatagcttta gaaaatctag
     1501 caactaaaac tgaaatggat agacttatta agtcagctct agtagatcct aaggaagaaa
     1561 atgttgatag agactataag aaggttcttg aattattgca tgatgaggct gtatatatcc
     1621 cattaactta tcaatcggtg atttctgttt ataggaaagg tgattttaaa accatgcgtt
     1681 ttgcgccaga agaaaattca ttcccattac gctatataga aaaaaacaat gtatctaagt
     1741 agttcgatca tcaaaaaaat attgtcagct tttttggcat tgtttttcat atcacttcta
     1801 acatttattc ttatcaaact atcaacagtt aatccagcag aaaattacct tcgtttatca
     1861 aaaataagtg ttagtccaga agcgctaaaa gaagcagagc actatttagg tttagataag
     1921 ccattatgga agcagtactg gctgtggttc caaaaagcat taacaggaga ttttggatat
     1981 tcttatgtgc tcaggttgcc agtattagat ttggttttac aacggttttt ggctacttta
     2041 tttttaggca cgagtgcctt cttgctaatt gttaccattt ctaccccgtt aggtgtgtgg
     2101 gctggcttac atgaatctgc tcggagtgat cacttgattc gatttttgag tttttctagt
     2161 gtgtctatgc caaatttttg ggttgcttac ttgttgatgc tgctattttc ggcaaaatta
     2221 aatcttctgc ctgtttcagg aggtaacgat ttacaaagtt taattttgcc aagtattacc
     2281 ttaagttttt ctaccgtagg tcaatacata gcccttattc gaaaagctat tagtcaagaa
     2341 aatagaagtt taaatgtgga aaatgcccga ttaagaggag taaaagaacg ttatattgtc
     2401 acacatcacc tccttagaaa tgccttacct gcgataatga cagcacttag cttgacttgg
     2461 gtttacttat taacaggatc aattattgtt gaagaaattt tttcatggaa tggaatagga
     2521 cgtttatttg tgaccagtct aagaacttca gatcttccag taatacaagc ttgtatgcta
     2581 atttttggaa ccttattttt agctaataat tttatgacac agtgttttat gaattgggtt
     2641 gatcctcggt tacgaaagtc aagggagaaa taatatcaaa aagaatttat ttttcgagta
     2701 gcttactggt attgttagtg attagtgcca tttttgcccc tattctatct agctttgatc
     2761 cacaatacgt agatttatca caaaaattat tggctcccaa taatgttcat ttgttaggga
     2821 ctgaccaatt gggtagagat gtattatctc gcttgcttta tggtgctaga tattcactgt
     2881 ttttagctat tattattagc ttattggaat taacgattgg tatgtttgtg ggtcttattg
     2941 ttggttggta tcaaggaaag ttagaaaacc tctttttatg gatagctaat attattttag
     3001 cttttccgag ctttttgctc tctcttgcaa ccgtggggat cttaggtcat ggtctaggga
     3061 atttaatttt tgcaattgtc tttgtggaat gggtttacta tgctaaatta atgaccaatt
     3121 tagttaagag tgcaaaaaaa gagccttatg tgataaatgc gcaaattatg ggactttcag
     3181 tctggcatat tttaagaaaa catatttttc cttttgtgta tcagccaatc ctcgttatgg
     3241 tccttatgaa tataggaaat attattttaa tgatttctgg cttttctttc ttagggattg
     3301 gtgtgcaacc taatgtcaca gaatggggaa tgatgttgca cgatgctaga ggatatttta
     3361 ggacagctac ttggatgatg ttatctcctg gaattgcgat ttttttaaca gtattttcct
     3421 ttaatacttt aggcgatgcc atagataaaa aagattggaa acgacaatgg aacagttaga
     3481 aataagaaaa ttgtcattac agataggaga ggtccctgta ctaagagatt ttagttgtaa
     3541 aatagatatg ggagaatcgc taactattat tggtgagagt ggttcaggga aaaccctctt
     3601 ggcaaaatta ttggttggtc acattccaca aggtatgaca gttagaggaa acatattttt
     3661 taagggagtt gatttaggta aactaactgt aaagcagtgg cagaaattaa ggggacgaga
     3721 tattgcctat ttagtgcaaa atcccatgtc tatgtttaat ccttttcaaa aaattgaagc
     3781 gcatattttg gaaacaatcc ttagtcatga gaaatgttca aagagagtag ccttatctaa
     3841 agcgttagaa tggatgaaac gtttaaattt agatgatgcg atatccctgt taaaaaaata
     3901 cccttttgag cttagtggag gaatgctaca aagaattatg ttagccacta tattatctct
     3961 tgatcctcag gtaatcatat tagatgagcc gacctcagcg gttgattgtc ataattgttc
     4021 tactatatca gctattttgc aggaattgca aaataatgga aaaaccttga ttactgtaac
     4081 gcatgattat cagttagcta gagacctggg gggacaatta ttagttataa gtgagggaga
     4141 agttgttgaa cagggacaaa cccaagctat tttaagcaat cctcaacata actatacgaa
     4201 agcactgaca gtgcaaatgg agtatgaagg agatattctt aatgttggtt tgtaatcatg
     4261 tcggaaaaac atttggacga caagaggttt taaaggactg tcattttcat cttaaaagag
     4321 gagaaataat tggtataatg ggtaaaagtg gcagtggtaa aagtagcctt gcccgactca
     4381 ttataggact agatagtcct acttgtggtt cgatatattt tcaaggaaaa atttacacac
     4441 ctaaagatgg taaggcgcag attattcttg tttttcaaga tgcgcttagt tcggtcaatc
     4501 catattttag tattgaggaa attttgaatg aagcttttta tggaaaaaaa acaacttttg
     4561 aattatgtca aatattagaa gcggtgggtt tagatggaac ttatctaaaa tataaagcta
     4621 gacaacttag tggtggtcag ctacagagag tttgtatcgc tagagcttta ctcttaaagc
     4681 ctaaaattat tatttttgat gaatccttaa gtggattaga cccagtaact caaattaaaa
     4741 tgctacgttt attacaaaaa ataaaacgcc gttatgaatt aagttttata atgatttctc
     4801 atgaccctaa aatttgtcaa gctatctgta accgggtctt tctgataaaa aatggttatt
     4861 tagtagaaga taatgaattc ttgaagagag cgtgttctac taactgtttg accaatctct
     4921 gaattattat gtttttctta ttttaggagt tgataaagaa atcaacttac tagtgtcatt
     4981 gctctattaa aattttgcct tttatggtaa aataataaag acaaaataag gagaacatag
     5041 tagtggaacc taaatatcaa cgtatattaa ttaagttatc tggtgaagca ttggcgggag
     5101 ataagggtgt cggtattgac attcctaccg ttcaatctat tgcaaaagaa attgccgaag
     5161 tacacaattc aggtgttcaa atagcacttg tcattggtgg aggtaatctt tggcgtggag
     5221 aacctgcagc agaagcaggg atggatcgtg ttcaagctga ttatacaggg atgttaggga
     5281 cagtaatgaa tgctcttgta atggctgata gccttcaaca atatggggta gatactagag
     5341 ttcaaacagc tattccaatg caaactgttg ctgagcctta cgtacgtggt cgtgctttac
     5401 gtcatctaga gaagaatcgt attgttgtct ttggagcggg tattggttcg ccttatttct
     5461 caactgatac aacagcagcg cttcgtgctg ctgagattga ggcagaagca attttaatgg
     5521 ctaaaaatgg agtagatggt gtttataatg ctgatccaaa gaaagatgcc aatgctgtca
     5581 aatttgatga attaactcac gttgaagtaa taaaacgagg gttaaaaatc atggatgcaa
     5641 ccgcatcaac tatttcaatg gataatgata ttgaccttgt tgttttcaat atgaatgaga
     5701 ctggtaatat taagcgtgtt gttcttggag aacaaatcgg aacaactgtt tcaaacaaag
     5761 catctgaata ggagtgtata aaaaatatga ctaaggaaat cgttacaaaa gctcaagagc
     5821 gttttgagca atcacaccaa agtttatcaa gagaatttgc agggattcgt gcaggacgtg
     5881 ctaatgctag cttgcttgat cgtattcaag ttgagtatta tggagcgcca accccactta
     5941 accaattagc ttctattact gtacctgaag ctcgtgttct tttaatttca ccatttgata
     6001 aatcatcaat caaagatatt gagcgtgcta ttaacgaatc agatttaggt attaatcctg
     6061 caaatgatgg ttcagttatt cgtcttgtta ttccagcttt gacagaagaa acacgtcgtg
     6121 atttagctaa agaagtgaaa aaagtcggag aaaatgctaa gattgctatc cgtaatattc
     6181 gccgtgatgc tatggatgaa gctaagaagc aagaaaagaa taaagaaatt acagaagatg
     6241 acttgaaatc tcttgaaaaa gatattcaaa aggctactga tgatgctgta aaacatattg
     6301 atgagatgac tgctaataaa gaaaaagaat tgttggaagt ttaatagtaa cgtttgctat
     6361 ctttattatg attggcaagt aataaaatga ttatggaggt aactgataga atcaaaacca
     6421 gatggatttg gtttgtcggt taccttttta aaaggagaaa aatgaataca ttattagcga
     6481 cagtcataac aggcttagta actgatgaaa acaaagattt ttactttatc caaaaagatg
     6541 gctttacctt tgcattatca aagtcagaag gagagcatca cattggtgaa atggttaaag
     6601 gattcgccta tacagatatg caacaaaaag cacgtcttac aactaaagaa acatttgcga
     6661 ctcgagatca ttatggttgg gggacagtta ctgaggtacg taaagacttg ggagtatttc
     6721 ttgatactgg tcttccagat aagcaagtgg ttgtttcttt ggatgttctt cctgaattga
     6781 aagaactttg gcctaaaaaa ggtgatcgcc tctacgtttg cttagatgtt gataaaaaag
     6841 atcgtctctg ggctctccct gcagatcctg aagtgtttca acgaatggca acccctgcat
     6901 ataacaatat gcaaaatcaa aattggccag caatcgttta tcgcttaaaa ttgtcaggaa
     6961 cttttgttta tctaccagaa aataatatgc tcggctttat tcaccctagt gaacgttaca
     7021 gtgaacctcg tttagggcaa gtgctggatg cacgagttat tggcttcaga gaagttgata
     7081 gaaccttaaa tttatcatta aagcctcgtt cttttgaaat gttagaaaat gacgcacaaa
     7141 tgattttaac gtatttagaa agtaacggag gattcatgac tttaaatgat aaatcttctc
     7201 cagaggaaat taaagctact tttggaattt ccaaaggtca gtttaaaaaa gcacttggag
     7261 gactcatgaa agctaaaaag atcaaacaag atcagctagg tacagaatta ttataaactt
     7321 ttgaactttt aaagttttct cacgaggtcg ttttagttat agctagaatt aatggctaat
     7381 tatagaaaaa agctgagagg aaattttggc tttttttgtt ataatgtatc tattaaaaag
     7441 aatggagaac gatatggaac gtgcgatttt tgctggaggt tgtttttggt gtatggtaca
     7501 accatttgaa gaattagatg gtattgaatc tgttttatct ggatacactg gaggacatgt
     7561 tgagaatcca acttataaag aagtttgtag taaaactact ggacatacag aagctgttga
     7621 aattattttt aatcctgaaa aaatctctta tgcagattta gttgagttat attgggcaca
     7681 aacagatcca acagacgctt ttggtcagtt tgaagatcgt ggggataatt atcgtcctgt
     7741 catcttttat gagaatgaag agcaacgtca gattgctcaa aaatcaaaag ataagctaca
     7801 agcttcgggt agatttgata gaccaattgt aaccagcatt gagcctgctg atacttttta
     7861 cccagcagaa gactatcatc aagcattcta tcggactaac cctgctcgtt acgccttgag
     7921 tagtgccaga agacatgcat ttctagagga gaactggcat tgagaaaatc attttacagt
     7981 tggttgatga cacaaagaaa tccaaaatca aatgaaccag tagcaatcct agcagattac
     8041 gcttttgatg aaaccacttt tccaaaacat agttcggatt ttgaaacagt tagtcgctat
     8101 ttagaagatg aggccagttt ttcctttaat ttaacagatt ttgatgatat ttgggaagat
     8161 tatctcaatc attaacaata atatagagat tgtattttca atctcttttt taggttatag
     8221 aaattggaca cattgtctaa gactgtctga aaagcaggtt ttttaatggc aatcgttttc
     8281 acaaaggagt agaatatagt catctaaaat atagccctag ggaggttgtt tatgagatat
     8341 acaaatggaa attttgaagc ctttgcaaga cctcgaaaac ctgaaggtgt ggataaaaaa
     8401 tccgcttata ttgttggttc tggtttagca ggattagctg ccgctgtctt tttaatacgt
     8461 gacggtcaaa tggatggtca acgtattcat atttttgaag aactacctct ttctggagga
     8521 tcacttgacg gtgtcaaacg acctgatatc ggttttgtaa cgcgtggtgg tcgtgaaatg
     8581 gaaaatcact tcgaatgtat gtgggatatg taccgttcca tcccctctct cgaagttcca
     8641 gatgcttctt atctagatga attttattgg cttgacaagg atgatcccaa ttcatctaac
     8701 tgtcgcctca ttcataaaca ggggaatcgc ttagaatctg atggtgattt tacactcgga
     8761 acacattcca aagagttagt taagctagtc atggagactg aagagtcttt aggtgctaag
     8821 acgattgaag aagttttttc aaaagaattt tttgaaagta atttttggac ttattgggct
     8881 actatgtttg cctttgagaa atggcattca gcgattgaaa tgcgtcgata tgctatgcgc
     8941 tttatccatc atattggtgg tctgcctgat ttcacttcat taaaatttaa taaatataat
     9001 caatatgatt ctatggtgaa accaatcatc agttatttag agtctcataa tgtagatgtt
     9061 caatttgata gcaaggtaac taatatctcc gtagacttta agaacggtca gaagcttgca
     9121 aaagccattc atctcacagt tggtggtgaa gcaaaaacaa ttgatcttac tccaaatgat
     9181 tttgtttttg taacgaatgg ttctatcact gaaagtacga actatggtag tcatgatact
     9241 gtagctaaac ctaataccga tttgggaggt tcttggaatc tttgggaaaa cttagctgct
     9301 caatctgatg aatttggaca tcctaaagtc ttctacaaag atattccaaa agagtcatgg
     9361 tttgtttctg ctactgcaac aataaaagat ccagctattg aaccttatat agagcgttta
     9421 acacatcgtg acctccatga tggtaaagta aataccggag gcattgttac agttacagat
     9481 tctaattgga tgatgagttt tgctatccat cgccaaccgc atttcaaaga gcaaaaggaa
     9541 aatgaaacaa ttgtttggat ttatggactc tattcaaatg tggaaggtaa ttatattaaa
     9601 aaaccaattg aagaatgtac tggtcgagaa atcacagaag agtggttata tcacttaggt
     9661 gttcctgaaa tgaagattca tgatttatct gataaacaat atgtaagtac agttcctgtt
     9721 tatatgcctt acattactag ttatttcatg cctcgagtta aaggtgaccg accagatgtt
     9781 atcccacaag gatctgttaa cctagccttc attggtaatt ttgcagaatc tccatccaga
     9841 gatacagtat ttacaacgga atattctatt agaactgcta tggaagctgt ttatactttc
     9901 ttaaatattg aacgtggtgt tccagaagtc tttaattcag catttgatat tcgtgtcttg
     9961 ttgcaatctt tatactatct caatgataag aaatctgttg aagatatgga tttaccaata
    10021 cctgccttaa tgcgtaaagt aggcatgaaa aaaattagag gaacttattt agaagaatta
    10081 cttcgagaag ctcatctttt ataatgtaaa ataaaatcga gaaaacttgt ttttctcgat
    10141 tttttagttt tacaacgact aaaatcacca aagaaaatga aattttgtta taatgtagtc
    10201 acttactgta cgaaaaggaa gaataatttg caagaatact ctatcgaaat tactttacaa
    10261 catccagatg atatgatgtc actctttggc agcaatgagc gccatttaaa attaatcgaa
    10321 gaaaatttag atgttatcat tcatgctaga actgaacgtg ttcaggtatt aggtgactct
    10381 gaagaagcag tagaaaccgc tcggttaacc attgaagcat tattagtgct tgttaaccgc
    10441 gggatgactg tgaatacttc agatgttgta acagccttat ccatggcaca aaatggttca
    10501 attgataaat tcgttgccct ttatgaggaa gaaattatta aggatagcta tggtaagcct
    10561 atccgtgtta agactttagg gcaaaaaata tacgttgata gcgttaaaaa tcatgatgtt
    10621 gtttttggta ttgggcctgc tggaacggga aaaacttttt tagcagtcac tctagcagta
    10681 acggcactaa agcgtggtca agttaaacga ataatcttaa cgagaccagc ggttgaagct
    10741 ggtgaaagcc ttggtttctt gcctggagat ctcaaagaga aagttgatcc ttatttgaga
    10801 ccagtttatg atgccctata tcaaatttta ggtaaagagc aaacaagtcg cttaatggag
    10861 cgtgaaatca tcgaaattgc tccattggct tacatgcgtg gtcgtacttt agatgatgca
    10921 tttgtcatat tagatgaagc gcaaaacacc actataatgc aaatgaaaat gttcctaaca
    10981 cgtcttggct tcaattctaa aatgattgta aatggcgatg ttagccaaat tgacttacca
    11041 aagaatgtta aatctggttt gattgatgct gttgagaaat tacgaaatat caaaaagatt
    11101 gactttattc atttatcagc aaaggatgtt gtaagacacc ctgtcgttgc tgaaataata
    11161 aatgcttatt cagattcaga aagtagtcac aagctacaat ctgataagga ctgatgtttg
    11221 ccactaggag acgttgttag agagtgtgtc aatatgtaaa aaacgcctaa tgctttaggc
    11281 gtttttatga tggtgtactt caacttaacc tataaagtgt tgaaaatttt ctttaagcat
    11341 gacgtcgaga tgcattatta tatcgtttaa tgtttcttta acagtgtgtt cgttggagtt
    11401 gtgatgacat agtttaatga tattttcttc gaaatgttta ggaagcaatg tagcattctt
    11461 cttagcatag gaaatcattc ttttttcgcc aggatgtgtc aacttattaa gtgcaaaaat
    11521 aatatcaaag taggaatcta agaaagcagt agttctatgg tgagtactaa caaagtcttg
    11581 gcgtttaagg gcttttataa tttgtttatc gtaagaggga agcttgccag ttaataagtt
    11641 acgattttga atgataattt gtttttgtaa ctgactggga taagaaactt cgaatctctc
    11701 tttgagtgat tgtaattgat tttcaggatc atagagtatt tggcaattga tgaggttatg
    11761 ccaaaaacaa gtagtgtatc caattcgaga attgtggtgt tcaacgacat cttctaagtc
    11821 tgataaaaag ttatctatgt tacgatagag aatatcaata tcagttccgt cgtttaaagt
    11881 accattatct tcaagctccc agtaatgatt gttaagttca atgtaatgac agtgcttgtt
    11941 aaggatacgt ttacgactag tgatatcagg agttgctgca caataaacat acaaatcata
    12001 atcggaagat tggtcaaaac tgtcaccaga gcgtgagcct ccgagtgcta tagcaacaac
    12061 atttggaagt ttagagaaat cttgccagag ttttgtgaaa tggtccataa atgctctcct
    12121 aaaaatgtaa aatattgatg tgtctccatt ctacagggct atctcttttt agtcaataat
    12181 taatagtaag ttaataaaag aggtaacggt tctaatattc aagaagtgta tatgagtgat
    12241 ttgaaaagga gatattttat gctacaatac gtacatatta gtttaaacaa ggtgatagaa
    12301 tgaaacaaga ttatatttcc tacattaggt caaaagttgg acatgagact atctttttaa
    12361 catattctgg tggtattttg acagatggta agggacgtgt cttactccaa ttaagagctg
    12421 ataagaattc ttggggaatt attggtggac gtatggagtt aggtgagtca tcagtagata
    12481 cattgaaaag agaatttttc gaagaaactg gattgagagt ggaaccaatc agactgttaa
    12541 atgtttatac taactttcaa gactcttatc ccaatggcga taaagcacaa accgtcggtt
    12601 ttatttatga agtaagctgt ccaaaaccag ttaatataga aggttttcat aatgaggaaa
    12661 cattgcaatt ggactatttt tctaaagaag acgtcaaaaa tattacgatc gtaaatgaac
    12721 aacaccaatt aattttagat gaatattttt cacaaacatt ccaaatgggg cgttaaaagg
    12781 atgggaaata ctaggtgtga ctttaaagaa cttctaactt ttattgatat gttgttaggc
    12841 attcttgtag aaacaaatgt taagaaaata aggagttctc atttgtatat tttataatta
    12901 ttaaaaagaa aaacagtagc ttgcaataat ttagtgactg tttttttaga aagcataaga
    12961 agtacagaaa aagaaaatta tggaatattg gatggtgggg atatgcttta cttacggtat
    13021 tggaatgata tacttaaaga aatccagttt tagaaatcca aatttctaag taaaatagca
    13081 taaaggagag tagatgaatg aagataacat tacacggcgt tgctgaaacg ttattaatca
    13141 ccttatatat tagggctaaa gatgcgatgg ctaaacatcc gatacttaat gatcaaaaat
    13201 ccttagcaat tgttgaacag atagaatatg attttgataa attcgataat tcagaagctt
    13261 ctttttatgc aacattagct agaattcgcg ttatggatag agaaatcaaa aaatttatta
    13321 gagaaaatcc aaatagtcaa atcctttcaa ttggttgtgg acttgataca aggtttgaaa
    13381 gagtcgataa tggacaaatt aggtggtata accttgattt gccagaggtt atggagataa
    13441 gaaaattatt ttttgaagag catgaaagag ttactaatat agcaaaatca gccctagatg
    13501 aaacttggac acgggaggta aatccccaaa atgccccttt tctaatcgtg tcagaaggtg
    13561 ttttaatgtt tctaaaagaa gatgacgtag agacttttct tcatatcctg acaaattcat
    13621 ttagccaatt tatggcacaa tttgatttgt gtcataagga aatgattaat aaaggaaagc
    13681 aacatgatac agtaaagtat atggatacag aatttcagtt tggtatcaca gatggtcatg
    13741 agattgtgga tttagaccct aaattaaagc aaataaatct gattaacttt acagatgaga
    13801 tgagcaaatt tgagttaggc acacttcgct ctttacttcc aacaattcgt aaatttaata
    13861 attgtttagg tgtgtacgaa tataaagcat ctgagaaaaa gtaacaaaaa tctatttata
    13921 taaagagaca ttctaaatgt aaatttgtca taatcgttat agcatttgta gctttgttta
    13981 tttaggaatt agttttacgt aagttaggcg ctctaaagtt tgtattttga agtaaatact
    14041 aattcataag tcagaatttt attgaaatgg attataagtg tgtaatagtg attattagct
    14101 ttctctccct tttttaatac tcttatggta taatgatgtg aaagaatagc ttgaaggaag
    14161 gaagagaacg cacttttatt ttgagaacta gttttagata aaagtgaaaa acttaggaaa
    14221 gtagatgtgt cgattattta gaattatgca ttacacttta tcttttctaa acgttttggg
    14281 tcgttcttac tatataagat gtacgttgaa atgattgatg aaactggaca agtatcagaa
    14341 gatatcaaaa agcaaactct tgatttgtta gagtttgcag cacaaaaaac aggtaaagag
    14401 aataaagaaa tggctgtaac gtttgtcact aatgaacgta gccatgaatt gaatttagag
    14461 tatcgagata ccgatcgtcc gactgatgtt atcagtttag aatataagcc tgaggttgat
    14521 atttcttttg atgaagaaga tctggctgaa aatcctgaac ttgctgagat gctagaagat
    14581 tttgattctt atattggtga actttttatt tctattgata aagctaaaga acaagcagaa
    14641 gagtacggtc actcttatga acgtgaaatg ggttttttag ctgtacatgg ttttttgcat
    14701 attaacggtt atgatcatta tacacccgaa gaagaaaaag agatgttcag cttacaggaa
    14761 gaaattttaa ctgcttatgg acttaaacga caataatcaa aaaaaatgga aaaatcgcac
    14821 cttaacatca agcatggaat ttgctgttac agggattttt acagctttta aagaagaaag
    14881 aaatatgcgt aagcatttgg tttctgccat tttagtaata cttgctggtc ttacttttca
    14941 agtatctatg gtggagtggt tatttctttt attgagtatt tttttggtaa ttacttttga
    15001 aattatcaat tcagctattg aaaatgtagt tgatttggca agtaattacc atttctcgat
    15061 gctggctaaa aatgctaaag atatggcagc aggagcggtt ttagttgttt ccttatttgc
    15121 cgtattggtg ggattgatta tttttatacc caagatactt gctttacttt tatgatgaga
    15181 agtattacga ataaattgaa tggagaaaga aaagaaatga catttaagtc aggatttgta
    15241 gcaattttag gtcgtccaaa tgttggaaaa tcaaccttcc taaaccatgt tatgggacaa
    15301 aaaattgcta tcatgagtga taaagctcaa acaacacgta ataaaattat ggggatttat
    15361 acaacagaaa ctgagcaaat tgttttcatt gataccccag gaattcataa accaaagacc
    15421 gcccttggtg attttatggt tgaatctgct tatagtactt tacgtgaagt tgaaacagtt
    15481 cttttcatgg ttcctgccga tgaaaagcga ggcaaaggcg atgatatgat tattgaacgt
    15541 cttaaggctg ccaagattcc tgttatttta gtaatcaaca aaattgataa ggttcatcca
    15601 gaccaacttt tggaacagat tgatgacttc cgtagtcaaa tggattttaa agaagtagtg
    15661 ccaatttctg cccttcaagg aaataatgtg ccaaccctaa tcaagttatt gactgataat
    15721 cttgaagaag gtttccaata tttcccagaa gatcagatta ctgatcatcc ggaacgattt
    15781 ttagtatcag aaatggttcg tgagaaggta cttcatttaa cgcaacaaga ggttcctcac
    15841 tcagtagctg ttgttgtcga atccatgaaa cgtgatgaag aaactgataa agtgcatatt
    15901 cgtgcaacta tcatggttga acgtgatagt caaaaaggca ttatcattgg aaaacaaggt
    15961 gctatgctca agaaaatagg aaaaatggcg cgccgtgata ttgaacttat gttaggtgat
    16021 aaagtttacc tagagacttg ggtaaaagtc aagaaaaact ggcgtgataa aaagcttgat
    16081 ttagctgatt ttggctataa tgaaaaagag tattaatgag tttattttga gatccgcaag
    16141 ttagtgggga agagttagat aagttcaccg tgagacttga ttcgcacaga aaaagcaatt
    16201 cacgaggata aaaccaacta gtcataattt tgagattata ttcacaaatt aattaaaggg
    16261 agccaaacat gcttgttggc cccttcttaa ataaaggaga gcacatgact caagattata
    16321 tttgctacct ataaagtaga ggaggtgagg atatgcataa tatattaaga tttttaggca
    16381 ttgttattat ttctgcagtg attctattta gtataggtag tttctacgat ttatcattaa
    16441 tgaaaaatat actacttatc tgttggagtt ttctctttga tctattggtt tttgtcttca
    16501 aacaaagaca aacgacagaa gtactcacat ggtatcaagt tgttaagcaa ttttggttat
    16561 ttattaagtg tactattctt attcctatct tagttgcttt tatt
//
LOCUS       DATROD010000032        16289 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.032, whole genome shotgun sequence.
ACCESSION   DATROD010000032 DATROD010000000
VERSION     DATROD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16289
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.032"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(17..394)
                     /gene="mscL"
                     /locus_tag="VBC74_000994"
     CDS             complement(17..394)
                     /gene="mscL"
                     /locus_tag="VBC74_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEO6941956.1"
                     /translation="MIKELKEFLFKGNVLDLAVAVILGAAFNAIITSLVKDVITPLIL
                     NPVLKAAGVSNIAQLSWNGVAYGNFLSAVINFLIVGTTLFFIVKAANKVMAKKPAEEE
                     IIEVVEPTQEQLLAEIRDLLANK"
     gene            517..2391
                     /gene="dnaG"
                     /locus_tag="VBC74_000995"
     CDS             517..2391
                     /gene="dnaG"
                     /locus_tag="VBC74_000995"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEO6941957.1"
                     /translation="MKKLLKKFTQKRKNDIIKCKTIMGYFCGGHDLAIDKEKISEIKN
                     SVNIVDVIGEVVGLTKTGRNHLGLCPFHKEKTPSFNVIEDRQFFHCFGCGRSGDVFKF
                     VEDYQHISFLDSVQVLAERSGIPLDTNFKGQVPKKPKANQSLLDIHRVASGFYHAYLM
                     TTNDGERARQYLAERGVTEDLIKHFQIGLSPGGQDFLYRRLAKEFDEKTLMSSGLFNY
                     SENSNQFYDSFNNRIMFPLTNDIGEVIAFSGRVWTQEDIDRKQAKYKNSRATPIFNKS
                     YELYHLDKARAVINKAHEVYLMEGFMDVIAAYRAGIENVVASMGTALTNEHVRHLKRF
                     TKKVVLTYDGDRAGQNAIDKSLELLSDMTVDIVRIPNKMDPDEFLQANSAEDFKQLLE
                     NGRISNTEFYIHYLKPENTDNLQSEIAYVEKIAKLIAKSPSITAQNSYITKVAELLPD
                     FDYFQVEQSVNNERLHHRSQQQASSSVQTSATVQLPQTGKLSAITKTEMQLFHRLLNH
                     PYLLNEFRNRDNFYFDTTEIQVLYELLKESGEITSYDLSQESDKVNRTYYIILEEQLP
                     VEVSIGEIEAVEKARDRLLKERDLRKQSQLIRQSSNQGDEEGALAALENLIAQKKNME
                     "
     gene            2399..3508
                     /gene="rpoD"
                     /locus_tag="VBC74_000996"
     CDS             2399..3508
                     /gene="rpoD"
                     /locus_tag="VBC74_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019787874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEO6941958.1"
                     /translation="MAEKKGNTTFNVQVADFIRNHKKQGTAIDDEVTEKLVIPFVLDA
                     DQIDDLLERLTDGGISITDKEGNPSTKYVVEGPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELAVAVAEGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLKDFMED"
     gene            3617..3958
                     /locus_tag="VBC74_000997"
     CDS             3617..3958
                     /locus_tag="VBC74_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEO6941959.1"
                     /translation="MSEVKQYSEEEVGKIKDRILEALEMVIDPELGIDIVNLGLIYEI
                     RFEDNGRTEIDMTLTTMGCPLADLLTDQIHDVMKTVPEVTETEVKLVWYPAWSVDKMS
                     RYARIALGIRS"
     gene            4048..4902
                     /gene="rfbD"
                     /locus_tag="VBC74_000998"
     CDS             4048..4902
                     /gene="rfbD"
                     /locus_tag="VBC74_000998"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEO6941960.1"
                     /translation="MILITGANGQLGSELRHLLDERTQEYVAVDVAEMDITNAEMVDK
                     VFEEVKPSLVYHCAAYTAVDAAEDEGKELDFAINVTGTENVAKAAAKHDATLVYISTD
                     YVFDGEKPVGQEWEVDDLPDPKTEYGRTKRMGEELVEKYTSKFYTIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQHGRPTWTRTLAEFMTYLAENQKDFGYYHLSNDAKEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLEKAKATGFVIPTWQDALKE
                     FYKQEVKK"
     gene            5019..6173
                     /locus_tag="VBC74_000999"
     CDS             5019..6173
                     /locus_tag="VBC74_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEO6941961.1"
                     /translation="MQDVFIIGSRGLPARYGGFETFVSELINHQKSSDIKYHVACLSD
                     KEHHTHFNFADADCFTINPPQLGPARVIAYDIMAINYALDLVKTHDLKEPIFYILGNT
                     IGAFIWHFANKIHKVGGLLYVNPDGLEWKRSKWSRPTQRYLKYAEKCMTKNADLIISD
                     NIGIENYIQSTYSNVKTRFIAYGTEINSRKLSSDDPRVKQLFKKWNIKSKGYYLIVGR
                     FVPENNYETAIREFMASDTKRDLVIICNHQNNPYFEKLSLKTNLQQDKRVKFVGTLYE
                     KDLLDYVRQQAFAYIHGHEVGGTNPGLLEALANTDLNLVLDVDFNKSVAGLSSFYWTK
                     KEGDLAKLINDSDQQQDLSTYGDRAKAIIQENYTWKKIVEEYEDLFLNES"
     gene            6163..7104
                     /locus_tag="VBC74_001000"
     CDS             6163..7104
                     /locus_tag="VBC74_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6941962.1"
                     /translation="MKVNILMATYNGEKFLAQQIESIQKQTFKEWNLLIRDDGSSDKT
                     CDIIRNFTAKDSRIRFINENEHHNLGVIKSFFTLVNYEVADFYFFSDQDDVWLPEKLS
                     VSLEAAKHKASDVPLLVYTDLKVVNQELNILQDSMIRAQSHHANTTLLPELTENTVTG
                     GTMMINHALAEKWFTPNDILMHDWFLALLAASLGEIIYLDLPTQLYRQHDNNVLGART
                     MDKRFKILREGPKSIFTRYWKLIHDSQKQASLIVDKYGDIMTANDLELIKCFIKIDKQ
                     PFMTRLRWLWKYGYSKNQFKHQVVFKWLIATNYYNKR"
     gene            7106..7837
                     /locus_tag="VBC74_001001"
     CDS             7106..7837
                     /locus_tag="VBC74_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6941963.1"
                     /translation="MMKKILLVIPAYNEEGSIAKTVQTIVDFKASRSLPFELDYIVIN
                     DGSTDGTPELLDRLGLNHIDLVQNLGIGGCVQTGYLYANRNHYDVAVQFDGDGQHDIR
                     SIEDVVMPILNDEADFVIGSRFVDKKHQNFQSTAMRRLGINLISAAIKLTTGHKVYDT
                     TSGYRAANAALIAYLSCHYPVQYPEPESTARILKKGYRLKEVTANMFEREAGTSSISS
                     LKSIFYMTDVLTSIIIAGFIKEDDK"
     gene            7834..8187
                     /locus_tag="VBC74_001002"
     CDS             7834..8187
                     /locus_tag="VBC74_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEO6941964.1"
                     /translation="MSLALQIEMFILAIVLLYMIIRMVNKNTFSVKRSLPWLLVGLIL
                     VFISVFPGVVSFIAHHLGFALTMNFLLFGAILFLFCLELFDTSSNTKRDEQIKRLIQE
                     VSLLKKEIEDSTLDE"
     gene            8180..9913
                     /locus_tag="VBC74_001003"
     CDS             8180..9913
                     /locus_tag="VBC74_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HEO6941965.1"
                     /translation="MSKKHIFICLFFMLFLVGCQNNKKVKTDKKVDITAYYYHNNTPQ
                     YSVQINAIHLQTALDKVRTGEFESKDFTHYTFDSIKRKYQVSGKKVLMDNNYRFGSGI
                     KNTRQDAIAIVRLLLEKNKDYLIYMNGLEEPSVLYNPENKRYKFTNNKGKAIGNIPVG
                     LTAFENSAETQEYVLKNIQKNETIYLGNTRVDNPRVTVNNKKRDTIGVEYGKRITYRI
                     PIYSKQLTVRVSPNFVVDSTNYNYRLSQAPIIAEEGKEGRLYPIDSSISVDGDKIVLN
                     SSGDETEQKELLQNSLYKLHSIDKLDFDLTNADIKELEITGHVASKRDYNLSVAQEKK
                     GVIETKRSLISVDNQKQDQGIFVEDSKGYIKTPSLSSYGINFAMYDDNQNRLLQGSEY
                     ILGRLNADKKVSLYQPNGFWINTQKTIKSLNKEYLNQNAYHIRGNNIIGIDGYSNDID
                     LNPRLWAYNYKKQVKSNQSLFKLRGLSNKYKYFLIQTKSPKGYLQSNKPFYFTVNKDS
                     VSKAQFGNYNINGYIMDMQYNKQEYNALANTPKGQKTKKTFKPMTLVILFAVALFIFY
                     ITAIRFVFKRM"
     gene            9938..10762
                     /locus_tag="VBC74_001004"
     CDS             9938..10762
                     /locus_tag="VBC74_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEO6941966.1"
                     /translation="MKEMTVSEIREVQLEMLAYIDKVARDNKIEYSLGGGSLLGAMRH
                     KGFIPWDDDIDLMLERSQYERLMKALADANNSDFKLLHHSVEKNLWPFAKLYHTKSMY
                     LSKTDRIHPWTGIFIDIFPLDRLPESAEERQRFFKKVHSAAANLMCTTYPNFASGSRK
                     LYANARLILGLPRFIAYHGQAKKRAEIVDQVMETYNNQEVPYMGYTDSRYRLKEYFPR
                     EIFSEYEDVMFENIKTRKIKNEHAYLNQLYGGSYMELPPESKRESHSYYTWYWKEG"
     gene            10762..11484
                     /locus_tag="VBC74_001005"
     CDS             10762..11484
                     /locus_tag="VBC74_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEO6941967.1"
                     /translation="MMNIGVIFAGGVGRRMNTKGKPKQFLEVHGKPIIVHTIDIFQNT
                     EAIDAVVVVCVSDWLDYMNNLVERFNLTKVKAVVAGGETGQMSIFKGLEAAEQLATDD
                     AVVLIHDGVRPLINEEVINANIQSVKETGSAVTSVRAKETVVLVNDSSKISEVVDRTR
                     SFIAKAPQSFYLSDILSVERDAISKGITDAIDSSTLMGMYNRELTIVEGPYENIKITT
                     PDDFYMFKALYDARENEQIYGM"
     gene            11484..12542
                     /locus_tag="VBC74_001006"
     CDS             11484..12542
                     /locus_tag="VBC74_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEO6941968.1"
                     /translation="MMIKFSNKIVQEDLALISNQNYINWERIANKGVLISGSNSMLAS
                     YMVFLLAYLNETRNYQTQIIATARNIEKARDKFSDLVGKDYFTLIPYDVEERLEYDGK
                     VDYIIHAASNASPTAILSNPVSIIKANTIGTLNLLDFAKEKTIENFLFLSTREVYGTS
                     IKEVIDEEAYGGFDILATRACYPESKRMAETLLQSYYDQYKVPFTIARIAHSFGPGME
                     LGNDGRIMNDLLSNVIDGKDIVLKSSGTAERAFCYLADAVSGLFTILLNGEVGQAYNV
                     ANEDQPIMIKDLAQKLVDLFSDKNISVVFDIPKTMSAGYSKMGRTRLTMAKLEALGWK
                     REVSLESGILKTVQAFEE"
     gene            12544..13479
                     /locus_tag="VBC74_001007"
     CDS             12544..13479
                     /locus_tag="VBC74_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6941969.1"
                     /translation="MVKTAVLMATYNGEKFISEQLDSIRQQTLKPDYVLLRDDCSTDE
                     TVNVVNNYIAKHELEGWKIVKNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDIWYL
                     DKNERQFAIMSDNPQIEVLSADVDIKTMSTEASVPHFLTFSSSDRISQYPKVYDYQTF
                     RPGWTIAMKRDFAQAIADNWTFGANISHDNLIETTASIIGNGYNLNESVGVHIRYGGN
                     ASGNSKFVLNWKSPKTQHITGLKTFVDFYETMQKVVNKYQNDVALNQRLAFEKERLEA
                     SKLSLIHNSAFVIKNWRLYNSNSGRMRDLLFAFKK"
     gene            13488..14435
                     /locus_tag="VBC74_001008"
     CDS             13488..14435
                     /locus_tag="VBC74_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6941970.1"
                     /translation="MVKTAVLMATYNGQGFIHDQLDSIRNQTLRPDYVLMRDDGSTDD
                     TVKVVEDYIKEHRLDGWSITSNDKNLGWRLNFRQLLIDVLAYEVDYVFFSDQDDTWYH
                     HKNKMQVDIMEERQDINLLSADIDIKKLSEEATIPNNFKFNGEETISKYPLDFSYHNY
                     RQGWTFCLRKSFVDQVMTFYSDNLILSHDNLFAGVSGVIGSGYNLNRSVGEHKRHAEN
                     ASGNILNMSSSYERHLQELYFVLSYYIILVPFLEKSSSSQLREAKAYLDFNQQRYKNA
                     QNKDFVAILKQMMTKKRFYDSFSNRIRDLVFLLKDIKRS"
     gene            14437..15801
                     /locus_tag="VBC74_001009"
     CDS             14437..15801
                     /locus_tag="VBC74_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEO6941971.1"
                     /translation="MNLKKVRLFEKFFLDKNLHKTYFILGLTITIVLSLLMPFFNEPD
                     GQYHLAVSGRISNNIIDTSYYGEYNIDSGMRRQSTSYRDGTRFEKYYLNKANFVDGNH
                     LPREINYSKHNFVFWGHLVPAVGLFLGKFVYPSMGVMITVARLFSSFVSITLVALIIR
                     YVKKGKLIYFAIFLSPVVLNSFASLSYDSTGYIVFGAFLSLLINLLVDKSLTKNRSIA
                     LIGLILLVIVACKQNYWLFLLLVIPVLLQVNNPKLMGYNQKLSHLFQGLKSKKLFWLC
                     LTFLSLAIVYVLTARHGGVLAVLRRYVMTFGYNYAGINILSNDITSWLASPYPSQNFI
                     PTWVSSVWYLMVFLVWFSEEKFVKSKNMGFLFLASFFVGILGVFFIMLEYRGAPTSYI
                     EGVQGRYFTVTLLLVQVFASSIPARLNEAGRKVVPYALTGLVIVSNALLLFDTVISLI
                     MRRN"
     gene            15801..>16289
                     /locus_tag="VBC74_001010"
     CDS             15801..>16289
                     /locus_tag="VBC74_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEO6941972.1"
                     /translation="MKLLKNMFYNTSYQLLTLLLPLVTVPYVSRVLSPQGIGINAYTS
                     SIVMYFTLFGALGISLYGNREIAFVQSNKYKRSKIFWELVVLKLASVSIATLLFFGFV
                     LLTNEWQLFYLIQGINLLATATDISWYFIGVEDFKIIVIRNTIVKLITVVLTFLVVKT
                     PDD"
BASE COUNT         5488 a         2380 c         3176 g         5245 t
ORIGIN      
        1 taatccgagt cttttattat ttgttagcga gtaagtcgcg gatttcagca agtaattgct
       61 cttgtgttgg ttcaacaact tcaataattt cttcttcagc aggtttctta gccataacct
      121 tgttagcagc tttaacgatg aagaataaag tagtaccaac gattaagaaa ttaataactg
      181 cacttaagaa attaccgtaa gcaacaccat tccatgaaag ctgtgcgata tttgaaacgc
      241 cagcagcttt taatacagga ttaaggataa gcggtgtaat aacgtcttta acaagtgaag
      301 tgatgatagc attaaaagct gcacctaaaa taacagcaac agcaaggtct aaaacattac
      361 ccttaaataa aaattctttt agttctttaa tcatgtgaac aagctcctta atattattta
      421 attcacgttc attatagcag atttcttttg agaaatccaa gtaataggtt tcaaattatc
      481 tgtaaatttt atattaaatc agtgaagaag aaatccttga aaaagctttt gaaaaaattt
      541 acacagaaaa gaaaaaatga tataatcaaa tgtaaaacta taatgggtta tttctgtgga
      601 ggtcatgact tggcaattga taaagaaaaa atttctgaaa tcaagaacag tgttaacatt
      661 gttgatgtca tcggtgaagt tgtcggtttg acaaaaacag gacgcaatca tcttggttta
      721 tgcccatttc ataaagaaaa aacgccatct tttaatgtta tcgaagatag gcagtttttt
      781 cattgttttg gttgtggtcg ctctggtgat gtctttaaat ttgtggaaga ttaccaacat
      841 attagttttt tagatagtgt acaagttcta gctgaacgtt caggaatacc tctagatact
      901 aattttaagg ggcaagttcc taaaaaacct aaagctaatc agtcgctttt agatattcat
      961 agagttgctt cgggatttta ccatgcctat ttaatgacaa ctaatgatgg tgaacgcgct
     1021 agacaatacc ttgccgagcg cggagtgaca gaggacttaa ttaaacattt tcaaatagga
     1081 ttatcgccgg gagggcagga ttttctatat cgtcgtctgg caaaagagtt tgatgaaaaa
     1141 actttgatgt cttctggttt gttcaattat tcagaaaata gcaatcagtt ttacgattca
     1201 tttaataata gaattatgtt tccgttaact aatgatattg gtgaagtcat tgcgttttca
     1261 ggacgagttt ggactcaaga ggacattgat agaaaacaag ctaaatataa aaattctcgt
     1321 gccaccccta tttttaataa gtcttacgag ctttaccatt tagataaagc gcgtgctgta
     1381 attaataaag cacacgaagt ttacctaatg gaaggcttta tggatgttat tgcagcttat
     1441 cgtgcaggaa ttgagaatgt tgttgcttct atggggaccg ctttaaccaa tgaacacgtt
     1501 agacacctta aaagatttac taagaaagtt gttctgactt atgatggtga tagagctggt
     1561 caaaacgcaa ttgacaagtc tttagaactg ctatcagata tgactgttga tatcgttagg
     1621 atacctaata aaatggaccc tgatgaattt ctacaagcaa attcagcaga agattttaaa
     1681 caactcttag aaaatgggcg catcagtaat acagagtttt atatccacta cttaaagcct
     1741 gaaaacaccg ataatttaca atctgaaatt gcttatgttg aaaaaatagc gaaattaatt
     1801 gctaaatctc catcgataac agcacaaaat tcatatatta caaaagtggc tgaactcttg
     1861 ccagattttg attattttca agtagaacaa tcggttaata acgaacgatt gcatcataga
     1921 agtcaacagc aagcttcatc ttcagtacaa acaagtgcta cagtgcaatt accacagaca
     1981 ggtaagttat cagctatcac taaaacagaa atgcaattgt ttcatcgatt attgaatcat
     2041 ccctacctgt taaatgaatt ccgtaatagg gataattttt attttgatac aacagaaata
     2101 caagttttat atgagttgct aaaagaaagt ggtgagatca cgtcttatga tttatcacaa
     2161 gaaagtgaca aagttaatcg tacttattat ataattttag aggaacaatt acctgtagaa
     2221 gtatctattg gagagattga agccgtcgaa aaggcacgtg accgtttatt gaaagaacgt
     2281 gatttaagaa aacaaagcca gttaattaga caatctagta atcaaggtga tgaagaagga
     2341 gccttggctg cattagaaaa tttaattgct caaaaaaaaa atatggaatg aggaacctat
     2401 ggcagagaaa aaaggaaata caacttttaa tgttcaagtt gcggatttta ttcgcaatca
     2461 taaaaaacaa ggtactgcaa ttgatgatga ggtaacagaa aaacttgtta ttccttttgt
     2521 tttagatgca gatcaaattg atgatttgtt agaacgattg acagacggtg gcatttcaat
     2581 cacagataaa gaaggcaatc cttcaacaaa gtatgtggta gaagggccaa aacctgaaga
     2641 attgactgat gaagagctca taggaagtaa ttctgctaaa gtcaatgacc cagtacgtat
     2701 gtacttgaag gaaattggtg ttgttcctct tttaacgaat gaagaagaaa aagaattggc
     2761 tgttgcggtt gctgagggag atttaatggc taaacaacgt ttagctgaag caaaccttcg
     2821 tcttgttgtc tcaattgcaa aacgttatgt tggacgtgga atgcaatttc ttgatttaat
     2881 tcaagagggt aacatggggc tcatgaaagc tgttgataaa tttgattatt caaagggatt
     2941 caaattttca acttatgcaa catggtggat tcgtcaagct ataacacgtg caattgcaga
     3001 tcaagcacgt acgattcgta ttcctgttca tatggttgaa acgattaata aattggtccg
     3061 tgaacaacgt aatcttcttc aagaacttgg tcaagatcca actcctgagc aaatcgcaga
     3121 acgcatggat atgacaccag ataaagtacg tgaaatccta aaaattgctc aagagccggt
     3181 atcgctagaa acgccaatcg gtgaggaaga tgatagccat ttgggtgatt tcattgaaga
     3241 cgaagttatt gaaaatccag tagattatac tacacgtgtt gttcttcgtg agcaattaga
     3301 tgaggtttta gatactctta ctgaccgtga agagaatgtt cttcgtttac gctttggtct
     3361 tgacgatggt aaaatgcgta cgttagagga cgtaggtaaa gtatttaatg ttactcgtga
     3421 gcgcattcgt cagattgaag ctaaagccct tagaaaactt cgccatccta gtcgtagcaa
     3481 gcaacttaaa gatttcatgg aagattgatt gcttctcgat aatcgttttt agaataggaa
     3541 gtttagtttc taataaaaat gatttgcaaa ggagttatct taaagacggt attaatattt
     3601 tcagagaggt attataatgt cagaagtaaa gcaatatagc gaagaagaag ttggaaaaat
     3661 aaaagatcgt atcctagaag ctttggagat ggttatcgat cctgaattgg gaatagatat
     3721 tgttaacctt gggttaattt atgagattcg ctttgaagat aacggtcgaa ctgaaattga
     3781 tatgacttta acgactatgg gatgcccgtt agcagattta ttgacagatc aaattcatga
     3841 tgttatgaaa acagtcccag aggtaactga aaccgaggtg aaattggtat ggtacccagc
     3901 ctggtctgtt gataagatga gtcgttatgc tcgtatcgct ttgggaattc gttcataaga
     3961 taaagagtaa agggatgaaa tattcccttt tttagtgtac aataaacgaa gaattaaaag
     4021 ttaaacttat ttttaaagga gcaaggaatg attttaatta caggggcaaa cgggcaatta
     4081 ggtagtgaat tacgtcacct tttagacgaa cgaactcaag agtatgttgc tgttgatgta
     4141 gctgaaatgg atattacaaa tgccgagatg gtggacaaag tctttgaaga ggtgaagcca
     4201 tctttagttt accattgtgc agcttacaca gctgttgatg cggcagaaga tgaaggaaaa
     4261 gagctagact ttgcaatcaa tgtcactggt acagagaatg tcgctaaggc tgctgctaaa
     4321 catgatgcaa cacttgttta catttcgacg gactacgtgt ttgatggtga aaaaccagtg
     4381 gggcaagaat gggaagtaga tgatctgcct gatcccaaaa cagagtacgg tcgtacaaaa
     4441 cgtatgggtg aagagttagt tgaaaaatat acttcaaaat tctataccat tcgcacagcg
     4501 tgggtatttg gtaactatgg taaaaacttt gtctttacga tgcaaaacct tgcaaaaaca
     4561 cacaaaactc taactgttgt taacgaccaa catggacgac caacatggac acgtactttg
     4621 gctgagttta tgacttattt agctgaaaat caaaaagatt ttggttacta tcatttatca
     4681 aatgatgcta aagaagatac aacatggtat gattttgcgg ttgaaatctt aaaagacaca
     4741 gatgtggagg ttaagcctgt tgattctagt caattcccag caaaagcaaa acgtccttta
     4801 aattcaacta tgagtttgga aaaagcgaaa gcaacaggat ttgtgattcc tacttggcaa
     4861 gatgctttaa aagagttcta caagcaggaa gttaaaaaat aagatcaaaa caaagagtag
     4921 taaggctaga gaatcctttt agcttactgc tctttttagc taacttttct aatttatggt
     4981 ataattgtat ggattgttta gctagaatgg agaagatgat gcaagatgtt ttcattatag
     5041 gaagtagagg gttgccagct cgttacggtg gttttgaaac ttttgtttca gaattgatta
     5101 atcatcaaaa aagttccgac ataaaatacc atgttgcatg ccttagtgat aaagaacatc
     5161 atactcattt taactttgct gacgctgatt gttttactat aaatcctccc caattagggc
     5221 cagcacgtgt gattgcttat gatattatgg ccattaatta tgcccttgac ttggttaaga
     5281 cacatgattt aaaagagcct attttttata ttttaggaaa tacaattggt gcctttattt
     5341 ggcattttgc caataaaata cataaagtcg gtggcttatt gtatgttaat ccggatggtt
     5401 tagagtggaa gcgatcaaag tggtctcgtc ccacacagcg ttatttaaaa tacgccgaaa
     5461 aatgtatgac taaaaatgca gacctaatta tttctgataa tattggtatt gaaaattaca
     5521 ttcaatctac ctactctaat gtgaagacaa ggttcattgc ttacggtaca gagattaatt
     5581 ctaggaaatt atcgtcagat gatccacgtg tcaaacagtt gtttaaaaaa tggaatatta
     5641 agtctaaggg ttactatcta atcgttggtc gatttgtccc tgaaaacaat tatgaaacgg
     5701 ctattaggga gttcatggct tcagatacta agcgtgattt agttattatc tgtaaccatc
     5761 aaaataaccc ctactttgaa aagttgtcct taaagacaaa ccttcaacaa gataaaagag
     5821 ttaagtttgt aggtacgctc tatgaaaaag atctgctgga ttatgttcgt caacaagcct
     5881 ttgcttatat tcatgggcat gaagttggcg gtactaatcc aggactgctt gaggctttag
     5941 ctaatactga tttgaatctt gttctagatg ttgatttcaa caaatcagta gcaggtctct
     6001 caagttttta ctggactaaa aaagaggggg atttagctaa gcttattaac gatagtgatc
     6061 aacaacagga tttatctact tacggtgatc gagcaaaagc cattattcaa gaaaactata
     6121 cttggaaaaa aattgttgaa gaatatgagg atttgttttt aaatgaaagt taatatttta
     6181 atggccacct acaatggaga gaagtttctg gcgcagcaaa tcgaaagtat ccaaaagcaa
     6241 acctttaaag agtggaacct cctaatcaga gatgatggtt caagtgacaa gacatgtgat
     6301 attattagaa actttactgc taaagattct cgaattcgtt tcattaatga aaatgagcac
     6361 cataatttag gagttattaa atcatttttt acgctagtta attacgaagt tgcagatttc
     6421 tattttttta gtgatcaaga tgatgtatgg ttgccagaaa agttatctgt aagtttagag
     6481 gcagcaaagc ataaagcatc tgatgtacca ttacttgttt atactgattt gaaagttgtt
     6541 aatcaagaat taaatatttt acaagacagc atgattcggg ctcaatcgca ccatgccaat
     6601 acgacattat taccagaact aactgagaat actgttactg gtgggactat gatgataaat
     6661 catgcattag ctgagaagtg gtttactcct aatgatatct taatgcatga ttggttttta
     6721 gcactcttag cagcaagttt aggagaaatc atttatttgg atttaccaac tcaactatac
     6781 cgtcaacatg acaataatgt tttgggggcg aggacaatgg ataagagatt taagatactt
     6841 cgtgaaggtc ctaaatctat atttacaagg tattggaaac ttatccatga ctcacaaaag
     6901 caagcttcct taattgttga taagtatggt gatattatga cagctaatga tcttgagctc
     6961 attaaatgtt ttataaaaat tgacaaacag ccttttatga caagattaag gtggttatgg
     7021 aaatatggtt atagcaaaaa tcaatttaag catcaagttg tgtttaaatg gttgattgca
     7081 acaaattatt acaataagag ataagatgat gaaaaaaatt ttactagtga tacctgctta
     7141 taatgaagaa ggtagtattg caaagacagt ccagactatt gtagatttta aggcgtctcg
     7201 ctcactacct tttgagctgg attatattgt tattaacgat gggtcaaccg atggtactcc
     7261 agagttatta gatagattgg gtttaaatca tattgacctt gttcaaaatc taggtattgg
     7321 tggctgtgtg cagacaggat acttatacgc taatcgtaat cattacgatg ttgccgttca
     7381 atttgatggt gatggtcaac atgatattag aagcattgaa gatgttgtta tgccaatctt
     7441 aaatgacgaa gcagattttg ttattggttc tcgctttgtt gataaaaaac atcaaaattt
     7501 tcaatcgaca gcaatgaggc gtcttggcat aaatttaata tctgctgcta taaaattaac
     7561 gacgggacac aaagtctatg atacaacaag tggttatagg gcagccaacg cggcgctaat
     7621 agcttattta tcgtgtcatt atccagtaca atacccagag cctgaatcaa ctgctcggat
     7681 tttgaaaaaa ggctatcgtt tgaaggaagt cacagctaat atgtttgaac gtgaggcagg
     7741 tacttcaagt ataagttccc taaaatcgat tttttatatg acggatgttt taacatcaat
     7801 tattattgca gggtttatta aggaggatga taaatgagtt tagcattgca aattgaaatg
     7861 tttattttag ccattgtcct actttatatg attataagaa tggttaataa aaacacattt
     7921 tctgttaaac gttctttacc ttggctatta gttggtttaa ttctagtctt tattagtgta
     7981 tttcctgggg tggtctcatt tatcgctcat caccttggat tcgctttaac gatgaatttc
     8041 ttgttatttg gtgctatttt atttctgttt tgtttagaat tatttgatac tagtagcaat
     8101 accaaaagag atgagcaaat taaacgcctg attcaagagg tttctttgtt gaaaaaagag
     8161 atagaggata gcactttaga tgagtaaaaa acatattttt atttgtctgt ttttcatgct
     8221 ttttttagta ggttgccaaa ataataaaaa agtaaaaaca gataaaaaag tagatattac
     8281 agcttattat tatcataata ataccccgca atacagtgtt cagattaatg ctattcattt
     8341 acaaacagca ttggataaag tgaggacggg ggagtttgag agcaaagact ttacacacta
     8401 tacttttgat agtatcaagc ggaagtacca agttagtggt aaaaaagtcc ttatggataa
     8461 taactataga tttggttctg gtataaaaaa tactagacag gatgctattg ctattgtaag
     8521 gttactgctt gaaaagaata aagattattt aatttacatg aatggcttag aagagccaag
     8581 tgttttatat aatccagaaa ataaacgtta taagttcaca aataacaaag gaaaagcaat
     8641 tggtaatatt ccagttggtt taactgcttt tgaaaacagc gcagaaactc aagaatatgt
     8701 gttgaaaaat attcagaaaa acgaaacgat ttatcttggt aatacccgag tagataatcc
     8761 tcgagtaact gttaataaca aaaaaagaga tactatagga gttgaatacg gtaaacgtat
     8821 tacttatagg attccaattt attcaaagca gctgactgtc agagttagcc ctaattttgt
     8881 agtagatagt actaattata attaccgact ttctcaagcg cctattattg cggaagaagg
     8941 taaagaggga cgtttgtatc caatagatag cagtattagt gtcgatggtg ataaaattgt
     9001 tttaaatagt agtggtgatg aaacagagca aaaagaatta ttacaaaata gcctgtataa
     9061 acttcactct attgataagt tagactttga tttgacaaat gctgatatta aagagttaga
     9121 gattacagga catgttgctt caaaaagaga ttataatttg tctgttgctc aggaaaagaa
     9181 aggcgtcata gagactaaac ggagtctaat ttctgttgat aatcaaaaac aagatcaagg
     9241 gatttttgtc gaggattcta aaggctatat aaagacacct agtttgagca gttatggtat
     9301 aaattttgcc atgtatgacg acaatcaaaa tagattattg caaggttcag agtatatttt
     9361 aggaagattg aatgcagata aaaaagtatc tctatatcaa cctaatggct tttggataaa
     9421 tactcaaaaa acgattaaga gtttaaataa agaatatctt aaccaaaatg cttaccatat
     9481 tagaggaaat aacattatag gtattgatgg ctattcaaat gacatagact tgaatcctag
     9541 gttatgggct tataactata aaaaacaagt gaaaagtaac caatcattat ttaaattacg
     9601 aggtttgtct aataagtata agtatttctt aatacagaca aaatctccaa aaggctactt
     9661 acaaagcaat aaaccatttt attttacagt aaataaagat agtgtttcta aagcacaatt
     9721 tggtaactat aatataaatg gttatataat ggacatgcag tacaacaaac aagaatacaa
     9781 tgcacttgca aacactccaa aaggtcaaaa aacaaagaaa actttcaaac ccatgaccct
     9841 agtgattctt tttgcagtag ctttatttat tttctatatt acagctataa gatttgtatt
     9901 taaaagaatg tgagttaact gttagacaga ggtaattatg aaagaaatga cggtctcaga
     9961 aataagagaa gttcaacttg aaatgttagc ttatattgat aaagttgcgc gtgataataa
    10021 aattgaatat tctttaggcg gaggcagcct actgggagca atgagacata aaggattcat
    10081 tccatgggat gacgatattg atttgatgct agaaagaagc cagtatgagc gtttgatgaa
    10141 agcattagct gatgctaata atagcgattt taaattgcta catcattctg ttgaaaaaaa
    10201 tctatggcca tttgccaagc tgtatcacac aaagtcaatg tacttaagta aaacagatag
    10261 aattcatcct tggacaggga tttttattga tatcttccca cttgaccgat taccagagag
    10321 tgcagaagaa cgtcaaagat tctttaaaaa agttcactct gctgctgcaa atttgatgtg
    10381 tacgacttac cccaattttg ctagcggttc ccgaaagctt tatgcaaatg caaggctaat
    10441 tttggggcta ccccgattca tcgcttatca cggccaagct aaaaaacgtg ctgaaattgt
    10501 tgatcaagtg atggaaacat ataataatca agaggttcct tacatgggtt ataccgattc
    10561 aagatatcgc ttgaaagaat attttccaag agaaattttt tctgagtatg aagatgttat
    10621 gtttgaaaac attaaaaccc gaaaaattaa aaatgagcat gcttatctga atcaattata
    10681 tgggggtagt tatatggaat tacctccaga aagtaagaga gaaagtcata gttattacac
    10741 ttggtactgg aaagaaggtt aatgatgaat attggagtta tttttgcagg tggtgttggt
    10801 cgtcgcatga ataccaaagg taaaccaaag caattcctag aagtgcatgg taaacccatt
    10861 attgtacaca caattgatat tttccaaaat acagaagcta ttgatgctgt agtagttgtc
    10921 tgtgtatcag actggctaga ttatatgaat aatctggtag agcgtttcaa ccttacgaaa
    10981 gtaaaagcag ttgttgcagg tggagaaact ggccaaatgt caatttttaa ggggcttgaa
    11041 gccgctgaac agctggctac agatgatgcc gttgttttaa ttcatgacgg agtacgtcca
    11101 ttgattaatg aagaagttat taatgctaat atacagtcag taaaagaaac aggaagtgcg
    11161 gtaacttcag ttcgtgctaa agagacagtt gttctagtca atgattcttc taaaatatct
    11221 gaagttgttg atcgtactcg ttccttcatt gctaaagcgc cacaaagttt ttatctatca
    11281 gatatccttt ctgttgaacg tgatgccatt agcaaaggga ttactgatgc aattgattct
    11341 agtactttga tgggtatgta caatcgtgaa ctcactattg ttgagggacc atatgaaaat
    11401 atcaagataa caacaccaga tgatttttat atgtttaaag ctttgtatga tgctagagaa
    11461 aatgaacaaa tttatgggat gtaatgatga ttaaatttag taataaaatt gtacaagaag
    11521 atttagcact tattagtaac caaaattata ttaattggga aagaattgct aataaaggcg
    11581 ttttaataag tggttctaat agtatgttag cgagttatat ggtattttta ctagcctatc
    11641 tcaatgagac aagaaattac caaacgcaaa ttattgcgac agctagaaac attgaaaaag
    11701 cccgtgataa attttcagat ttagttggga aagattattt caccttaatt ccttatgatg
    11761 tagaagaacg cctagagtat gatggaaaag tggattacat tatccatgca gcaagcaatg
    11821 ctagtccaac tgctattttg agtaatcctg ttagcatcat aaaagctaat acaattggta
    11881 cattgaattt attagacttt gcaaaggaaa aaacaattga aaacttcttg ttcttatcaa
    11941 cgcgtgaagt atatggaaca tctattaaag aagtgattga tgaagaagct tatggtggat
    12001 ttgatatttt agctactaga gcttgttacc cagaaagcaa acgtatggct gaaactctac
    12061 tgcaaagtta ctatgatcaa tataaggtac cctttactat tgctcgtatt gcacattcat
    12121 ttggtcctgg tatggagctt ggtaacgatg gtcgtattat gaatgatctt ttatcgaatg
    12181 tcattgatgg caaagatatc gttttaaaaa gtagtggtac agcagagcgt gctttctgtt
    12241 atttagcaga tgctgtatca gggttattta ctattttatt aaatggtgaa gttggtcaag
    12301 cttacaatgt agcaaatgaa gatcaaccca ttatgattaa ggatttggca caaaagttag
    12361 ttgatctctt ttctgataaa aatatttcag tagtattcga tattcctaaa actatgagtg
    12421 caggttatag caaaatgggg agaacacgtc tcactatggc caaattagaa gcgttgggat
    12481 ggaagcgaga agtttctctt gaatctggaa tattaaaaac agtacaagca tttgaggagt
    12541 aatatggtaa agacagcagt tttaatggcg acatacaatg gcgaaaaatt tatatctgaa
    12601 caacttgatt caattcgcca acagacatta aaaccagatt atgtattatt gagggatgat
    12661 tgttcaacgg atgaaacagt caatgtcgtc aataactata tcgcaaaaca tgagttagaa
    12721 ggctggaaaa ttgttaaaaa cgacaaaaac ttaggctggc gtttaaattt tcgtcaatta
    12781 cttattgatg tgttagccta tgaggttgac tatgtctttt ttagtgatca agatgatatt
    12841 tggtatcttg ataaaaacga acgacagttt gccattatgt cagataaccc tcaaattgag
    12901 gttttgagtg cagacgttga tatcaaaacg atgtctacag aagccagtgt tccacatttt
    12961 ctaacttttt cttctagtga tagaatcagt cagtatccta aagtatatga ttatcaaaca
    13021 ttccgtcccg gatggaccat tgctatgaag agagattttg cgcaagctat cgctgataat
    13081 tggacttttg gagctaatat ttcgcacgat aatcttatcg agacaactgc cagtattatt
    13141 ggaaatggtt ataaccttaa cgaaagcgta ggagttcata ttcgatatgg aggaaacgca
    13201 agtggtaata gcaaatttgt tttgaattgg aagtcgccta aaacacaaca tatcacagga
    13261 ttaaaaacat ttgttgattt ttacgaaacc atgcagaagg ttgtcaacaa atatcaaaat
    13321 gatgtcgctt taaatcaacg cttagccttt gaaaaagaaa gattggaagc ttctaaattg
    13381 tcattaattc ataatagtgc ttttgtgatt aaaaattggc gactctataa ctctaatagt
    13441 ggacgtatga gagatttatt atttgcgttt aaaaagtaag gaataatatg gtaaaaacag
    13501 cagttttgat ggcaacatat aatggtcaag gtttcatcca cgatcagcta gattcaatta
    13561 gaaatcaaac gttgagacca gactatgtct taatgaggga cgatggctct actgatgata
    13621 ctgtaaaggt ggttgaagac tatataaaag agcaccgttt agatggttgg tcaataacta
    13681 gtaatgataa aaatttaggt tggcgtttaa attttcgtca attacttatt gatgtgttag
    13741 cctatgaggt tgactatgtc ttttttagtg atcaagatga cacatggtat catcataaaa
    13801 ataagatgca agttgatatt atggaagaac gccaagatat aaaccttctt agcgccgata
    13861 ttgatatcaa aaagttatca gaagaggcta ctattcccaa taattttaag ttcaatggtg
    13921 aggaaacaat ttctaagtat ccattagact tttcatatca taactatcgt caaggttgga
    13981 ctttttgttt acgcaagtcg tttgttgatc aagtaatgac tttttacagt gataatttaa
    14041 ttttgtcaca tgataatctt tttgcagggg tatctggtgt cattggttca ggttacaatt
    14101 taaacagaag tgttggtgaa cataagcgac atgctgagaa tgcaagtggg aatatcttga
    14161 atatgagctc aagttacgaa aggcatttac aagaactgta ttttgttttg agttactata
    14221 tcatccttgt accattttta gaaaaaagta gctctagtca attaagggaa gctaaagctt
    14281 atctggattt taatcagcaa cgttataaaa atgctcaaaa taaagatttt gtagcaatac
    14341 ttaaacaaat gatgactaaa aaacggttct atgatagctt ttcaaatcgc attcgtgact
    14401 tggtgttttt attaaaagat ataaaaagga gttaatatga acctcaaaaa agttcgtcta
    14461 tttgaaaagt tttttctaga caagaacttg cataaaacat attttatatt aggcttaacg
    14521 attactattg tattgagcct attaatgcca tttttcaatg aacctgatgg acaataccat
    14581 ttagctgtgt ctggtcgtat ttcaaataac attattgata cttcttacta tggagaatat
    14641 aatattgatt caggtatgag acgacaatcg acctcctata gagatggtac tagatttgaa
    14701 aaatactatt taaataaagc caattttgtt gatggtaatc atttgcctcg tgagataaac
    14761 tattctaaac ataactttgt tttttggggt catcttgtgc cagcagttgg cttattttta
    14821 ggcaaatttg tgtatccttc tatgggggta atgattacag tagcaaggtt atttagttcc
    14881 tttgtctcaa tcaccttggt tgccctcatt atccggtatg tgaaaaaagg aaagttaatc
    14941 tactttgcta ttttcctcag tcccgtagtt ctaaattcat ttgctagtct atcttatgac
    15001 tcgactggat atattgtatt tggagcattt ttatcgcttt tgataaatct tttagttgat
    15061 aaatcgttga ctaaaaatcg tagtattgct cttataggat tgattctttt agtcattgtt
    15121 gcatgtaagc agaattactg gctgttttta ttgctagtaa taccggtatt attgcaggtt
    15181 aacaatccta aattaatggg ctataatcag aagctatcac atttatttca aggtcttaaa
    15241 tctaaaaagc tattttggct atgtctaacc tttttgtcac ttgctatagt ttatgttctg
    15301 accgcaagac atggtggtgt ccttgctgtt ttaagacgtt atgtaatgac atttggctat
    15361 aattatgctg gtatcaatat tttaagtaat gacattactt cctggcttgc ctctccatat
    15421 ccaagtcaga attttattcc cacttgggta tcatcagtat ggtatctcat ggtctttcta
    15481 gtatggtttt ctgaagaaaa atttgttaag tcaaaaaata tgggatttct tttcttagct
    15541 agtttctttg tgggaattct tggtgtcttt tttatcatgt tagaatatcg tggagcgcca
    15601 acaagctaca ttgaaggagt tcaaggacgt tactttactg ttacattgct tttggtacaa
    15661 gtttttgcat caagtattcc agcgcgtcta aatgaggcag gaaggaaagt ggtgccgtat
    15721 gcgttaactg gcttggtaat tgtatcaaat gccttattgt tatttgatac tgttattagt
    15781 ttaattatga ggagaaacta atgaaacttt tgaaaaatat gttttataat acctcatacc
    15841 agttattgac tttgttgtta cctttagtaa cagttcctta tgtttcccgt gtactatctc
    15901 ctcaaggcat cggtattaat gcctatacga gctccattgt catgtacttt actttatttg
    15961 gtgctttagg gatatctttg tacggtaatc gtgagatagc ttttgtgcag tcaaataagt
    16021 ataagagaag taagatattt tgggaattag ttgtattaaa acttgcctct gtatctatag
    16081 caacgttatt attctttgga tttgtcttac ttacaaacga atggcaatta ttctatttaa
    16141 ttcaaggtat taatttatta gcgactgcaa ctgatatttc gtggtatttt attggagttg
    16201 aagactttaa gataattgtc attcgtaata ccattgttaa gcttattaca gttgttttga
    16261 ccttcttagt tgtgaagacg ccagatgac
//
LOCUS       DATROD010000033        16214 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.033, whole genome shotgun sequence.
ACCESSION   DATROD010000033 DATROD010000000
VERSION     DATROD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16214
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.033"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            3..395
                     /locus_tag="VBC74_001011"
     CDS             3..395
                     /locus_tag="VBC74_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1310 domain-containing protein"
                     /protein_id="HEO6941973.1"
                     /translation="MKSIKLLSFIVIILLGLGFTVGGLKVTENNKQQELWEIANSKDA
                     KNAYQKWIYAEDEDAFKENAVIKSYDIDKESIKKNPMGGISVRLIINKDPNLYITCNL
                     DRDNQGHLVSQSSHQSPQLTHLLESRGH"
     gene            395..1687
                     /locus_tag="VBC74_001012"
     CDS             395..1687
                     /locus_tag="VBC74_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6941974.1"
                     /translation="MEKVLTDLDRVNIAKQEYELGSQLDTLVKIMSQDKVLPIGKVAH
                     VQDGGKETGEQIYTITPNGTLDKPEDVKEVTVLFKGSTAPFGGDDWKTDWFKNDIPIA
                     SKLLLKKFGSQSVSHKQGTKQLEQSAHLLKEVMNKYPNAKISVYGHSLGSMNAQYAIA
                     ELDDNQIYRVQGAWIYNGPNLYSILTDKQKEQVDKIKGVVNNYVDSKDKIGIGYPKEG
                     SDNAVGIVKIVDSKAVSGGSWVANFTNQHIWGGYQYNPDGSLKLAEDKFAYKENYSVV
                     HTNVNQRMHAIKAKLSSFKSTGKEVSKNQAIFLDSQQAKVISSGLVTISKEAVEEVKK
                     VEQKAVAKAEELYQSLKVAPTGVTELSPQELEEAYAQSGVSYASIVGEAQTFFSNKTQ
                     AAESISHAYVELENQINKGVEAMLRQDSNLSGEFKQWH"
     gene            1678..2070
                     /locus_tag="VBC74_001013"
     CDS             1678..2070
                     /locus_tag="VBC74_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941975.1"
                     /translation="MALIDIIEKQLADTQRKISDLDDAYHHSCCQFEEKLDDLSVRKN
                     KIINMLQETYDAVEYDLRYSNDSSDMMTLNRILGSYHDDLEQAYHKEYYALSAQEEEY
                     RANYIRQRSEHELTFEELQREKKRELMK"
     gene            2080..2361
                     /locus_tag="VBC74_001014"
     CDS             2080..2361
                     /locus_tag="VBC74_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941976.1"
                     /translation="MSLMWDVLTGKESVRTLKKQLDKELIHSVYAPRKRSKFHDIITS
                     HHKEIDEVAINTKTAIHKVANQLDEAIKGEFRSKVEEAIESNQQKYNHL"
     gene            2543..3325
                     /locus_tag="VBC74_001015"
     CDS             2543..3325
                     /locus_tag="VBC74_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEO6941977.1"
                     /translation="MIKIFDTHTHLNVENFEGKIDEEINLASELGVTKMNVVGFDQDT
                     ISKSLELSSQYAQVYSTIGWHPTEAGSYDDNIESMIISHLENPKVIALGEIGLDYYWM
                     EDPKDIQIEVFKRQIELSKEYNLPFVVHTRDALEDTYEVIKESGVGPFGGIMHSFSGS
                     LEMAQKFIDLGMMISFSGVVTFKKALDVQEAARELPLDKILVETDAPYLAPVPKRGRE
                     NKTAYTRYVVEKIAELRGITVEEVAEATYQNAVRIFRLDEKN"
     gene            3312..3872
                     /gene="rnmV"
                     /locus_tag="VBC74_001016"
     CDS             3312..3872
                     /gene="rnmV"
                     /locus_tag="VBC74_001016"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEO6941978.1"
                     /translation="MKKIDIQEVIVVEGKDDTANLRRFYNVDTYETRGSAIDEDDLER
                     IERLHNLRGVIVFTDPDYNGERIRKIIMNAIPTVRHAFLNRDEAKPGSKTKGRSLGVE
                     HASFEDLQKALSKVTQHFDDEDHFDITQADLIRWGFITASDSRKRREYLGNQLRIGYS
                     NGKQLLKRLRLFGVTKAEVEECMEGY"
     gene            3891..4382
                     /locus_tag="VBC74_001017"
     CDS             3891..4382
                     /locus_tag="VBC74_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6941979.1"
                     /translation="MEYNVTEALTRAGVDLATLAVKGTATMVTSKIQSIKDVKDAATL
                     RATYDDLINELLAEREQAIRIAQSYKEEYEKINIDDEDIEYLSNTLEQGISLLYNFSG
                     LNEEQERTMKQLVTLLNKDTLKTMQLIGFNYKQAIGEPLTEACATAIKNKFITQNSKG
                     KRK"
     gene            4462..5334
                     /gene="rsmA"
                     /locus_tag="VBC74_001018"
     CDS             4462..5334
                     /gene="rsmA"
                     /locus_tag="VBC74_001018"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEO6941980.1"
                     /translation="MRIADKTVTRAILERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DKGVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLIPILADTLARFDNVQVVNQDIL
                     KADLQTQIQAFKNPDLPIKVVANLPYYITTPILMHLIESKIPFAEFVVMIQKEVADRI
                     SAMPNTKAYGSLSIAVQYYMTAKVSFIVPRTVFVPAPNVDSAILKMVCRDQPVVSVQD
                     EDFFFRVSKVAFVHRRKTLWNNLTSHFGKSEDTKAKLEKALEIAKIKPSIRGEALSIP
                     DFASLADALKEVGI"
     gene            5338..6189
                     /locus_tag="VBC74_001019"
     CDS             5338..6189
                     /locus_tag="VBC74_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEO6941981.1"
                     /translation="MLVDKKWRFEDSASYFACPKCQNPLIKESNSLKCSDNHCFDLSK
                     FGYVNLLGGKKVDEHYDKKSFENRQLVLENGYYNHILEAISKVLENNSQFHSVLDIGC
                     GEGFYSRQLVNKHEKTFLAFDISKDSIQLAAKSDQSRLVKWFVSDLANLPIQDSSIDI
                     ILDIFSPANYKEFRRVLSDDGILVKVVPVAEHVQELREKASQYLKQKDYSNQKILDHF
                     RENFEIISEQKVVQSYNCSQQERQAFIDMTPLLFSVDKTTIDWASISEITVGALIVIG
                     KKRSVSK"
     gene            6345..7217
                     /gene="rsgA"
                     /locus_tag="VBC74_001020"
     CDS             6345..7217
                     /gene="rsgA"
                     /locus_tag="VBC74_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEO6941982.1"
                     /translation="MQGRIVKSLAGFYYVESDGVVYQTRARGNFRKKGQIPYVGDWVE
                     FSSQDQSEGYILSIEERKNSLVRPPIVNIDQAVVIMSAKEPDFNANLLDRFLVLLEYK
                     MIQPIIYISKLDLLDDLVVIDDIRELYQNIGYVFCYSQEELLPLLANKVTVFMGQTGV
                     GKSTLLNKIAPELKLETGEISGSLGRGRHTTRAVSFYNVHKGKIADTPGFSSLDYEVD
                     NAEDLNESFPELRRLSHFCKFRSCTHTHEPKCAVKEALTQGQLWQVRYDNYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            7224..7886
                     /locus_tag="VBC74_001021"
     CDS             7224..7886
                     /locus_tag="VBC74_001021"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEO6941983.1"
                     /translation="MSTNKIAPSILAADYANFANELKRIEETTAEYVHIDIMDGQFVP
                     NISFGADVVSSMRKHSKLVFDCHLMVVDPERYIEAFAQAGADIMTIHVEATKHIHGAL
                     QKIKEAGMKAGVVINPGTPVESLIPILDLVDQILIMTVNPGFGGQAFIPEMMSKVKTV
                     AAWRKEYGHHYDIEVDGGIDNTTIKAAAEAGANVFVAGSYLFKASDLPAQVETLRVAL
                     DD"
     gene            7879..8511
                     /locus_tag="VBC74_001022"
     CDS             7879..8511
                     /locus_tag="VBC74_001022"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEO6941984.1"
                     /translation="MTKIALFAGGDLTYFEYDFDYFVGIDRGSLFLLKNGLSLDMAVG
                     DFDSITEDELLYIKHYCSNIVSASAEKNDTDTELALKTIFKEFPEAQVTVFGAFGGRI
                     DHMMSNIFLPSDRDLEPFMSQIRLKDEQNIVTYLPSGKNQVSRIEGMSYVSFMPESES
                     TLQISGAKYELNKSNYFKKKMYSSNEFMTSPIEVELKDGYLIIIYSKDRG"
     gene            8513..9787
                     /locus_tag="VBC74_001023"
     CDS             8513..9787
                     /locus_tag="VBC74_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEO6941985.1"
                     /translation="MLVIILIIVLASLTVTIISYPKMTELTKSVEKQLEDNADNLSDQ
                     LTYQIEVAQKDQILTLTNQLNRMQQEIYQLLTDMRTELNQHLTESRDRSDKRLELINS
                     NLSQSVQKMQDSNEKRLDQMRQTVEEKLEKTLQTRLQTSFETVSRQLESVNQGLGEMK
                     TVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTVSQYEREFPTVSGSSERVEYAI
                     KLPGNGQGDYIYLPIDSKFPLEDYYRLEDAYELGDKVQIELYRKSLLASIRKFAKDIN
                     NKYLNPPETTNFGIMFLPTEGLYSEVVRNATFFDSLRRDENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNANDISKILGNVKVEFGKFGGMLSKAQKQLNTASKSIDSLLTTRTNAI
                     IRVLNTVEEHQDQATTSLLNLPITEEEEINEN"
     gene            9777..10718
                     /locus_tag="VBC74_001024"
     CDS             9777..10718
                     /locus_tag="VBC74_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEO6941986.1"
                     /translation="MKINQMKKDELFEGFYLIKKAEVRKTRAGKDFIAFTFQDDTGEI
                     SGNMWDAQTYNVEEFVAGKIVHMKGRREVYNGTPQVNQITLRNIKDGEPNDPRDFKEK
                     PPINVDNVREYMEQMLFKIENATWQRVVRALYRKYNKEFFTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPELNKSLMFAGIMLHDLAKVIELSGPDNTEYTIRGNLIGHIS
                     LIDEELTKILAELNIDDTKEEVTVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALNRVNEGEMTNRIFAMDNRSFYKPNIK"
     gene            10815..11627
                     /gene="purR"
                     /locus_tag="VBC74_001025"
     CDS             10815..11627
                     /gene="purR"
                     /locus_tag="VBC74_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEO6941987.1"
                     /translation="MKLRRSERMVVISNYLINNPYTLTSLNTFASKYGAAKSSISEDI
                     AIIKKAFEQAQIGDIKTVTGASGGVIFTPTIAEAEAKEIVEELRQRLSENDRILPGGY
                     IYLSDLLSTPKMLQSIGRIIANAYRGQKIDAVMTVATKGVPLANAVANVLDVPFVIVR
                     RDLKITEGSTVSVNYASGSSGRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTVSGMISL
                     LSEFDSTLVGVAVFAENAQEQREKMAYKSLLRVSEIDVKNNRVSVEAGNIFD"
     gene            11846..12259
                     /gene="rpsL"
                     /locus_tag="VBC74_001026"
     CDS             11846..12259
                     /gene="rpsL"
                     /locus_tag="VBC74_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEO6941988.1"
                     /translation="MPTINQLVRKPRKSKVEKSDSPALNIGYNSHRKVHTKLSAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            12281..12751
                     /gene="rpsG"
                     /locus_tag="VBC74_001027"
     CDS             12281..12751
                     /gene="rpsG"
                     /locus_tag="VBC74_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEO6941989.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFEAIKEATGTDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            12906..14984
                     /gene="fusA"
                     /locus_tag="VBC74_001028"
     CDS             12906..14984
                     /gene="fusA"
                     /locus_tag="VBC74_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEO6941990.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQSLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYVDQANEYREKLVEAV
                     ADTDEDLMMKYLEGEEITNEELMAAIRKATINVEFYPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDEEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVLNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     SKVILESIEVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEARGNTQVVRAFVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNSGE"
     gene            15188..16198
                     /gene="gap"
                     /locus_tag="VBC74_001029"
     CDS             15188..16198
                     /gene="gap"
                     /locus_tag="VBC74_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEO6941991.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGQFVKVSAEREPANIDWATDGVEIVLEATGFFAS
                     KEKAEQHIHENGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALQDNFGVKQGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVATLEKDVTVEEVNAAMKAAANDSYGYTED
                     PIVSSDIVGISYGSLFDATQTKVQTVDGNQLVKVVSWYDNEMSYTSQLVRTLEYFAKI
                     AK"
BASE COUNT         5436 a         2677 c         3351 g         4750 t
ORIGIN      
        1 agatgaagtc aataaaatta cttagtttta ttgtaataat ccttttaggg cttggtttca
       61 ctgtaggagg tttaaaagtg actgaaaaca ataaacaaca agaattatgg gaaatagcta
      121 atagcaaaga cgctaaaaat gcttatcaaa agtggattta tgcagaagat gaagatgctt
      181 tcaaagagaa tgctgttatt aagtcgtatg atattgacaa agaaagtatc aaaaaaaatc
      241 ctatgggagg gatttcggta aggttaatca ttaataaaga tcctaactta tatattactt
      301 gtaatttgga tagggataat caagggcatt tggtcagtca aagtagccat caatctcccc
      361 agttaactca tttattggaa agtagaggtc attaatggaa aaagtcttga ctgatttaga
      421 tagggtaaat atagcgaagc aagagtatga attaggtagc caacttgata cactagtcaa
      481 aataatgagc caagataagg tactccccat cggtaaagtt gctcacgttc aagatggagg
      541 caaagaaacc ggggaacaaa tttataccat cactccaaat ggaactttag acaagccaga
      601 ggatgttaag gaagttacgg ttttatttaa ggggtcaaca gcaccgtttg gaggggatga
      661 ttggaaaaca gactggttta aaaatgatat tccaatagct agtaaactat tattaaagaa
      721 atttggttca caaagtgtat ctcataaaca aggtacaaag cagttagaac aatcagctca
      781 tctgctaaaa gaagttatga ataaatatcc taacgcaaaa atttcggtct acggacactc
      841 gttagggtca atgaatgcac agtatgctat tgcagaattg gatgataatc aaatttaccg
      901 agttcaagga gcttggatat ataacggtcc aaacctttat agtattttga cagataaaca
      961 aaaagaacag gttgataaaa taaaaggggt ggtcaataat tacgttgatt ccaaggataa
     1021 aattggaata ggttatccta aagaaggaag cgataatgct gtcggaattg ttaaaatagt
     1081 tgacagtaaa gctgtttcag gaggtagctg ggttgctaat tttaccaatc agcatatttg
     1141 gggaggctac cagtacaatc cagatggttc tttaaaatta gcagaagata aatttgctta
     1201 caaagaaaac tattcagtag ttcatacgaa tgtgaatcaa agaatgcatg ctattaaagc
     1261 gaaacttagt agctttaaaa gtacgggaaa agaagttagt aagaatcaag ctattttcct
     1321 agatagtcaa caagcaaaag tgatctctag tggtttggta acgatatcaa aagaagcagt
     1381 tgaagaagtt aaaaaagttg agcaaaaagc agttgctaaa gcagaagagc tttatcaatc
     1441 tttaaaagta gctcctacag gagttactga actaagtcct caagaattgg aagaagcgta
     1501 tgctcagtca ggtgttagct atgcgagcat tgtcggagaa gctcaaacct ttttctcaaa
     1561 taaaactcaa gcggcagaaa gcatttctca cgcttacgtt gaattagaaa accaaattaa
     1621 taaaggagtt gaagctatgt tgcgacaaga tagtaacttg tcaggggagt ttaaacaatg
     1681 gcactgattg atatcattga aaaacaatta gctgatactc agagaaaaat atcagattta
     1741 gatgatgcct atcaccatag ttgttgccag tttgaagaaa aattagacga tctctcagta
     1801 agaaaaaata agattattaa tatgcttcaa gaaacttacg acgctgttga atatgatttg
     1861 agatatagta atgatagctc agatatgatg acgctcaatc gtattttagg ttcttatcat
     1921 gacgacttag agcaagctta ccataaagag tactatgcct tgtccgctca agaagaagaa
     1981 tatagagcta actatattcg gcaacgttca gaacatgaac taacctttga agagttgcaa
     2041 agggaaaaga aaagagaatt aatgaagtag gagaaattat tgagtttaat gtgggatgtc
     2101 ctgactggta aggagtcagt aaggacttta aaaaaacaat tggataaaga attgatacat
     2161 agcgtgtacg cgccacgcaa gagatctaag tttcacgata ttattacgtc gcatcataaa
     2221 gaaatagacg aagtagccat caacacaaag acagctattc ataaagttgc taatcaattg
     2281 gacgaggcaa taaaaggaga atttcgttca aaggttgagg aggctattga aagcaatcag
     2341 caaaaatata atcatttata gtctagtttc tctaccatta gctagacact tgtagaggat
     2401 ttttacagca agtattcaaa agtcagagca aaaatgtttt gacttttttg atttactctt
     2461 tctgatcaga atgcttcagt cttatgttat aataataaag attaatgctt gtagctacga
     2521 taagcaaaga aagaacgatg atatgataaa aatatttgat acccataccc accttaacgt
     2581 agaaaatttt gaaggaaaga tagacgagga aattaattta gctagcgaac tcggtgttac
     2641 aaaaatgaat gttgtcggct ttgaccaaga taccatttca aaatctttgg aattatcttc
     2701 ccaatatgca caggtatata gcacaatagg atggcatcca actgaagctg gttcttatga
     2761 tgataatatt gagtcaatga ttatcagtca tttagaaaat cccaaggtta ttgcacttgg
     2821 tgaaattggt ctggattatt attggatgga ggatcccaaa gatattcaga tcgaggtgtt
     2881 taagcgtcaa attgaacttt caaaagagta taaccttcct tttgtcgttc atacacgtga
     2941 tgcgttagaa gacacttatg aagtcattaa ggaatctgga gttgggcctt ttggtggtat
     3001 aatgcactct ttttctggtt ctttggaaat ggctcagaaa tttattgatt tagggatgat
     3061 gatttccttt tctggtgttg tgacctttaa gaaggcatta gatgtacaag aagctgcacg
     3121 agagttgcca ttagataaga tattagtgga aacagatgct ccttatttgg ctcctgtacc
     3181 taaaagaggt cgagaaaaca aaaccgctta tacgagatat gtcgttgaaa aaatagcaga
     3241 gctacgaggc ataactgtgg aagaggtagc ggaggctaca taccaaaatg cagtaaggat
     3301 atttagactt gatgaaaaaa attgatattc aagaagtgat cgttgtcgaa gggaaagatg
     3361 atactgctaa tctgcgacgc ttttacaatg ttgataccta tgaaactaga ggttcagcaa
     3421 ttgatgaaga tgatttagag cgtatcgaac gtttgcacaa tttgcgggga gttatcgtct
     3481 ttacagaccc agattataat ggcgagcgta tccgtaaaat catcatgaat gccattccga
     3541 cagtccgcca tgccttcctc aatcgagacg aggcaaagcc aggctcaaag accaagggac
     3601 gatctctggg tgtcgagcat gccagctttg aagatttgca gaaggcactc tcaaaagtga
     3661 ctcagcattt cgatgatgaa gatcatttcg atatcaccca agcagacctc attcgttggg
     3721 gattcattac tgctagcgat agccgaaaac gccgtgagta tcttgggaat caactccgta
     3781 tcggctactc aaatggtaaa caactcctca aacgcctcag actcttcgga gtgaccaagg
     3841 cagaagttga agagtgtatg gaggggtatt aatagaaaag gagaaaaata gtggagtata
     3901 atgtaacgga agcattgact agagctgggg tagatttagc aacgcttgca gtcaaaggga
     3961 ctgcaactat ggtaaccagt aaaattcaat caataaagga tgtaaaagat gctgcaacat
     4021 tgagagctac ttatgatgat ttaattaacg aattattagc tgaacgggag caagcaattc
     4081 gcattgcaca gtcatataaa gaagagtatg aaaaaattaa tatagatgat gaggatattg
     4141 aatatctatc caatactctc gagcaaggaa tctcattgtt atataatttt tctggtctaa
     4201 atgaggaaca ggaacgaaca atgaaacaat tggttacatt attgaataaa gatactttaa
     4261 aaacaatgca gttgattgga tttaattata agcaagcaat aggagaacca ctaaccgaag
     4321 cttgtgcaac tgcaattaaa aataagttta taactcagaa tagtaaaggc aaaaggaagt
     4381 agtggtataa tccagttgta atatcacaaa tagcctcaaa tgttatttca aaaatgatca
     4441 atattaagaa atagaggaat tatgagaatc gcagataaaa ccgttacccg cgctattttg
     4501 gagcgtcatg ggtttacatt taagaaatca ttcggtcaga actttttgac cgatactaat
     4561 attttacaga agattgttga cacggcagag attgacaagg gtgtcaacgt gattgagatt
     4621 ggtcctggta tcggggcttt gacagagttt ttggctgaga atgcagctga ggttatggct
     4681 tttgagattg atgatcgtct cattcctatc cttgctgata cgcttgcgcg ttttgacaat
     4741 gtccaagtgg tcaatcagga tatcctcaag gctgatttgc agacccagat tcaggctttc
     4801 aagaatcctg atctgcctat caaggtggtc gcaaacctac cttactacat cacaacacca
     4861 atccttatgc acttgattga gagcaagatt ccttttgcgg agtttgtggt catgatacaa
     4921 aaagaagtgg cagatcgtat ttcagctatg ccaaatacta aagcatacgg ttctttgtca
     4981 atcgcagtgc agtattatat gactgccaaa gtatcattta tcgttccgag aactgttttt
     5041 gttcctgctc caaatgtaga ttcagctatt ttgaaaatgg tctgtagaga tcaaccagta
     5101 gtgagtgttc aagatgagga tttctttttc agagtgtcaa aagtagcctt cgtacatcgt
     5161 cgtaaaacac tttggaataa tctaacgagt cattttggaa aatcagaaga tactaaagca
     5221 aaattggaaa aagcattaga aatagcaaaa attaagccat ctattcgcgg agaagcctta
     5281 tctatccctg attttgcaag tttagcagat gccttgaaag aggttgggat ataaactatg
     5341 ttggttgaca agaagtggcg ttttgaagat agtgcaagtt atttcgcttg tcctaaatgt
     5401 caaaatccct tgataaaaga aagtaacagc ctcaaatgct ctgataatca ttgttttgat
     5461 ttatccaaat ttggatatgt taatcttctt ggaggaaaga aggtagatga acattatgat
     5521 aaaaaatcat ttgaaaatcg gcagcttgtc ttagaaaacg ggtattataa tcatatttta
     5581 gaagctattt caaaggtttt ggaaaataat tctcagtttc attcggtttt agatattggt
     5641 tgtggagagg gcttttattc tcgtcaatta gttaacaagc atgagaagac tttcttggct
     5701 tttgatattt ctaaagattc gattcaatta gctgctaaaa gtgatcagtc taggcttgtg
     5761 aaatggtttg tttctgattt agctaacctt cctattcaag actcatcaat tgatatcatt
     5821 ttagacattt tttctcctgc aaattataaa gaatttaggc gggtgctgag tgatgatggt
     5881 atccttgtaa aggttgttcc tgtagctgaa catgttcaag aattgcgaga aaaagctagt
     5941 caatacttga aacagaaaga ttactctaat caaaaaattc tagatcactt tagagaaaat
     6001 tttgaaatta tttctgagca gaaagttgtt caaagctata actgttctca acaagaacgt
     6061 caagctttta ttgatatgac accgttgtta ttcagcgtag ataaaacgac tattgattgg
     6121 gcgagtatat cagaaattac agtaggtgcc ttaatagtaa ttggaaaaaa gcgcagtgta
     6181 tctaaataag atcactgctt ttatgttggg aattcataaa attgaggtta tttgagagaa
     6241 atcgaacacc tgcaaatagt ttctatttag cttatctctg agagtagagc ttatttctca
     6301 ttttatgata taatagtggt attctaaaca attaaggaga acaattgcaa ggtagaattg
     6361 tcaagtcgct agcaggtttt tattatgtgg aatctgacgg tgttgtttat cagacacgtg
     6421 cgcgtggaaa ttttagaaaa aaaggtcaaa ttccctacgt tggggattgg gttgaatttt
     6481 cttcgcaaga tcaatcagaa ggatatattc tatcaattga agagcgtaaa aatagcctcg
     6541 tacgaccacc tattgttaat attgatcaag ctgtcgttat catgtctgct aaagaacctg
     6601 attttaacgc taacttatta gaccggttct tagtacttct agaatataag atgattcagc
     6661 ctatcattta tatttccaag ttggatttgt tagacgattt ggttgttatt gatgatattc
     6721 gcgaacttta tcagaatata ggctacgttt tctgttattc tcaagaggaa ctcttgcctc
     6781 tgttagcaaa taaagtgact gtttttatgg gccagacagg tgtcggaaaa tcaacgcttc
     6841 tcaacaaaat tgctcctgag ttgaaacttg aaactggtga aatttcgggt agtttaggcc
     6901 gtggccgaca caccacaaga gcagttagtt tctataacgt tcataaagga aaaattgcag
     6961 acactcctgg tttttcttct ttggactatg aagtggataa tgcagaggac ttaaacgaat
     7021 cctttccaga gcttcgtaga cttagtcatt tttgtaaatt ccgctcgtgt acgcatacac
     7081 atgagcctaa atgtgctgta aaagaagcct taactcaagg tcagttatgg caagtccgct
     7141 atgataacta cctccaattt ttaagtgaaa ttgagaaccg tcgtgaaaca tataagaaag
     7201 ttattaaaag aaagtaggtt tctatgtcaa ccaataaaat tgccccatcg atattagcag
     7261 cagactatgc taattttgct aacgaattga aacgtattga agagacaaca gcagaatacg
     7321 ttcatattga tatcatggat ggtcaatttg tccctaatat tagttttggt gcggatgttg
     7381 tatccagcat gcgtaaacac agtaaacttg tttttgattg tcatctgatg gttgttgatc
     7441 cagaacgtta tattgaggct tttgctcaag ctggtgctga tatcatgacc attcatgttg
     7501 aagcaacgaa acatatacat ggtgcgcttc aaaagataaa agaggcagga atgaaagcag
     7561 gtgtggttat taacccagga acacccgtag aatctcttat tcctatctta gatttggttg
     7621 accagatttt aattatgact gttaatcctg gctttggtgg acaagccttt attccagaaa
     7681 tgatgtcaaa agtaaagaca gttgcagcat ggcgcaaaga gtacggtcat cattatgata
     7741 ttgaagttga tggcggtata gataatacta caattaaggc agcagcagag gctggtgcaa
     7801 atgtctttgt tgcaggttct tatctcttta aagcaagtga tttacctgca caagtagaaa
     7861 ccttaagggt ggcgctcgat gactaaaatt gctttatttg ctggcggtga cttaacttat
     7921 tttgaatatg attttgacta ttttgtcgga attgaccgtg gaagcctatt tttattaaaa
     7981 aatgggcttt ctttggacat ggcagtgggt gattttgatt ctataacaga agatgaactg
     8041 ctttatatca aacactattg tagcaatatc gtttctgcta gtgcagaaaa aaatgataca
     8101 gatacagaat tagctttaaa aactattttc aaagaatttc cagaagctca ggtgaccgta
     8161 tttggagcat ttggtggcag gattgaccat atgatgtcta atatcttttt accaagtgac
     8221 agagatctag aaccatttat gtcacaaatc agactcaaag atgaacaaaa catagtgact
     8281 tatctaccat ccgggaaaaa tcaagtctca aggattgaag ggatgtccta tgtctcgttc
     8341 atgcctgaga gtgagtctac tttgcagatt tctggggcta aatatgaatt gaacaaaagt
     8401 aattatttta agaaaaaaat gtattccagt aatgaattta tgacttcgcc aattgaagta
     8461 gagctcaaag atggttactt aatcattatt tatagtaagg atagaggtta atatgcttgt
     8521 catcattttg atcattgtac tagctagtct gacagtgacg ataatttctt acccaaaaat
     8581 gacggaatta acaaagtccg ttgaaaaaca acttgaagat aatgctgata atctatcaga
     8641 ccaactgaca tatcagatag aagtggcgca aaaagatcaa attctaacgt tgactaatca
     8701 gctaaaccgt atgcaacagg aaatttatca gttgcttacc gatatgcgta cagaattaaa
     8761 tcagcattta acggagagtc gagatcgatc agacaaacgc ttggaattga ttaactccaa
     8821 tttatctcag tcagttcaga aaatgcaaga ttccaatgaa aaacgcttgg atcaaatgcg
     8881 ccaaactgtt gaggaaaagc tagaaaaaac gctacaaacg cgtttgcaaa cttcttttga
     8941 aactgtatcg cgtcaactag agagcgtcaa tcaaggtctt ggggagatga aaactgttgc
     9001 ccaagatgtt ggtacactga acaaagtctt gtcaaatact aagacaaggg gaatattagg
     9061 ggagttacaa ctcggccaaa ttatagaaga tattatgaca gttagtcaat atgagagaga
     9121 atttcctacg gtgtctggct cttctgagcg tgttgaatat gctattaaat tacctggaaa
     9181 tggtcaggga gattatatct atttgcctat tgactctaag tttcctctag aagattatta
     9241 ccgattggaa gatgcttatg aattgggtga caaggttcaa atagaactct atcgtaaatc
     9301 tttactggca agtattcgta aatttgcaaa agatataaac aataagtact taaatcctcc
     9361 tgaaacgaca aattttggta tcatgttctt accaactgaa gggctctatt ctgaagtggt
     9421 aagaaatgca acattctttg atagtctaag acgtgacgaa aatattgtag tagctggacc
     9481 gtcaacctta tctgctttac taaattcatt atctgttggc tttaaaacct taaatattca
     9541 aaagaatgct aatgatatta gtaaaatttt agggaatgtc aaggttgaat ttggaaaatt
     9601 tggtggcatg ttatcaaaag ctcaaaaaca attaaataca gcaagtaaaa gtattgatag
     9661 tttactcaca acgcggacaa acgctattat cagagtctta aatacagttg aagaacatca
     9721 agatcaagca accactagct tgttaaacct tcctataaca gaagaagagg aaattaatga
     9781 aaattaacca aatgaaaaaa gacgagcttt ttgaggggtt ttaccttata aagaaagcag
     9841 aagttcgtaa aacgcgtgca ggtaaagact ttattgcctt tacctttcaa gatgatactg
     9901 gcgaaatttc aggaaatatg tgggatgcac agacctataa tgtagaagag tttgtagccg
     9961 gtaaaattgt tcatatgaaa gggcgtcgtg aagtttataa tggaactccc caagtcaacc
    10021 aaattacatt aagaaacatc aaagatggtg aacctaatga tcctagagat tttaaagaaa
    10081 aacctccaat caatgttgac aacgtgagag agtacatgga acaaatgctc tttaaaattg
    10141 aaaatgcaac ctggcagcgt gtggtaagag cactttatcg taaatacaat aaggaatttt
    10201 ttacctatcc agccgccaaa acaaaccacc acgcttttga atcaggattg gcatatcaca
    10261 cggcaacaat ggttcgtttg gcagatagta tcggagatat ctatccagaa cttaataaaa
    10321 gtttgatgtt tgctggtatt atgctacatg atttagccaa ggtcattgag ttatcgggtc
    10381 ctgataatac agaatatact attcgaggta atcttatcgg tcatatttca cttattgatg
    10441 aggaattaac aaaaatacta gcagagctta atattgatga tacaaaagaa gaggttacag
    10501 tgttacgcca tgttattctg agtcatcatg gacaattaga atatggaagc ccggttcgtc
    10561 ctagaatcat ggaggcagaa attatccata tgatcgataa cattgatgca aatatgatga
    10621 tgatgacaac agctttaaac cgtgtcaacg aaggtgaaat gacaaatcgc atttttgcca
    10681 tggataaccg ttctttttat aaaccgaaca ttaaataatt taggtagaat aattaagacc
    10741 taaaataaaa tgttcgtttt tagcatttgt tatgttatac tatatgtaaa gaatgctatg
    10801 aataaggaga aaatatgaaa ttaagacgta gcgaacgcat ggttgttatt tcaaattacc
    10861 taataaataa tccctataca ttaactagtt taaatacatt tgctagtaaa tacggtgccg
    10921 cgaaatcttc gatttcagaa gatattgcta ttatcaaaaa agcttttgaa caagctcaaa
    10981 tcggagatat taaaactgtg actggtgcaa gtggaggtgt tatctttaca ccaactattg
    11041 ctgaagcaga agcgaaagaa atcgttgaag aattaagaca gcgtttatct gaaaacgatc
    11101 gtattcttcc gggaggttac atttatcttt cagatctact cagtacacca aaaatgttac
    11161 aaagtattgg tcgcattata gcgaacgctt acagaggtca aaaaattgat gctgtcatga
    11221 cggttgcaac aaaaggtgtt cctttagcaa acgcagtagc aaatgtttta gatgttcctt
    11281 ttgttattgt gagacgtgat ttgaagatta cggaaggttc tactgtgtca gtaaactacg
    11341 ctagtggttc aagtggccgt attgaaaaaa tgtttctttc taagcgtagc ttaaaaccta
    11401 acagtcgagt tttaatagtt gatgatttct tgaaaggtgg aggtactgtt tcggggatga
    11461 ttagtttact ctcagaattc gatagcacat tagtaggtgt cgctgtattt gcagaaaatg
    11521 ctcaggaaca acgtgagaag atggcctata aatctcttct gagagtatct gaaattgatg
    11581 taaaaaataa tagagtctct gtagaagcgg ggaatatatt tgattaaaga gtcaaggtga
    11641 catccttgat tctttttgct tgtaaaagta ataaaagctt gacacattat gtgtaaaatg
    11701 atactatgat aaacggtact tttacttttg gtctctcaaa agtgtacaga gacgtgctga
    11761 caattgttgc aaaagtacac gcagatatag gctgtcacca agtgctatat caaccaaaaa
    11821 taaaaaactt acaggagaaa tgtagatgcc tacaattaac cagttggtac gtaaaccacg
    11881 taaatctaaa gttgaaaaat cagattcacc tgctttgaat attggttaca acagtcacag
    11941 aaaagttcac actaaattat ctgcaccaca aaaacgtggt gttgcaactc gtgttggaac
    12001 tatgacacct aaaaaaccta actctgccct tcgtaaattt gctcgtgtac gtttgagcaa
    12061 ccttattgaa gttactgctt acattccagg tatcggacac aacttgcaag aacacagtgt
    12121 tgtgcttatc cgtggtggac gtgtaaaaga ccttccaggg gtacgttacc atatcgttcg
    12181 tggtgcactt gatactgcag gtgtagctga tcgtaaacaa ggccgttcta aatacggtgc
    12241 taaacgtcct aaagggtaat tagaaggggg ataagagaaa atgagtcgta aaaatcaagc
    12301 gcctaaacgc gaagtattgc cagatccatt gtataactca aaaatcgtta cacgtcttat
    12361 caaccgtgta atgcttgatg gtaaacgtgg tactgctgca actatcgtat atgatgcttt
    12421 tgaagcaatt aaagaagcta ctggaacaga tgctcttgaa gtgttcgaaa cagctatgga
    12481 aaacatcatg cctgtacttg aggtacgtgc acgccgtgtt ggtggatcaa actaccaagt
    12541 tcctgtcgaa gttcgtccag aacgtcgtac aactttagga cttcgttggt tggttaacgc
    12601 atcacgtgct cgtggtgaac acactatgaa agatcgtctt gcaaaagaaa tcatggatgc
    12661 tgcaaacaac acaggagcat cagttaagaa acgtgaagat actcacaaaa tggctgaagc
    12721 taaccgtgcc tttgcacact tccgttggta agattactct ctttttgaga cttatcatag
    12781 ctactaaaca aattgtttgg tagatagcaa tgttagcaat tagaacgggt aggtcctgcc
    12841 tatccgtttt ttataaatag tgttataata aagaagtaaa taatttaaaa tataggagaa
    12901 accaaatggc tcgcgaattt tcacttgaaa aaactcgtaa tatcggtatc atggctcacg
    12961 tcgatgctgg taaaacaaca acaactgagc gtattcttta ctatactggt aaaatccaca
    13021 aaatcggtga aacacacgaa ggtgcttcac aaatggactg gatggaacaa gaacaagaac
    13081 gtggtatcac aattacatca gctgcaacaa cagcacaatg ggatggtcac cgtgttaaca
    13141 tcatcgatac accaggacac gtggacttca caattgaagt tcaacgttca cttcgcgtac
    13201 ttgatggtgc ggtaacagtt cttgatgctc aatcaggtgt agaacctcaa actgaaacag
    13261 tttggcgtca agcaacagaa tacggagttc cacgtatcgt ttttgctaat aaaatggata
    13321 aaatcggtgc tgacttcctc tattcagtac aatcacttca tgaccgtctt caagcgaatg
    13381 cacacccaat tcaattgcca attggttcag aagacgattt ccgtggtatt attgacttaa
    13441 tcaaaatgaa agctgaaatc tatactaatg accttggtac agatatcctt gaagaagata
    13501 ttccagcaga gtatgttgat caagcaaatg aatatcgtga aaaattagta gaagcagttg
    13561 ctgatactga tgaagatttg atgatgaaat accttgaagg tgaagaaatc actaacgaag
    13621 agttgatggc tgctattcgt aaagcaacta tcaatgttga attctaccct gtattgtgtg
    13681 gttcagcctt caagaataaa ggtgttcaat tgatgcttga tgctgttatt gattaccttc
    13741 caagcccact tgatattcct gcaatcaaag gtattaaccc tgatactgac gaagaagaaa
    13801 cacgtcctgc ttcagatgaa gagccatttg cagcgcttgc cttcaagatc atgacagatc
    13861 ctttcgtggg tcgcttaaca ttcttccgtg tgtactcagg tgttcttaat tcaggttcat
    13921 acgtgttgaa tacatctaaa ggtaaacgtg aacgtatcgg acgtatctta caaatgcacg
    13981 caaatagtcg tcaagaaatt gaaactgttt atgctgggga tatcgcagcg gctgttggtt
    14041 tgaaagatac aactacaggt gattcattga ctgatgaaaa atcaaaagtt attcttgaat
    14101 caattgaagt tccagaacca gttatccaat tgatggttga gccaaaatct aaagctgacc
    14161 aagataaaat gggtatcgcc cttcaaaaac ttgctgaaga agatccaacg ttccgcgttg
    14221 aaactaacgt tgaaactggt gaaacagtta tctcaggaat gggtgaactt caccttgatg
    14281 tccttgttga ccgtatgaaa cgtgaattca aagttgaagc aaacgttggt gcacctcaag
    14341 tatcataccg cgaaacattc cgcgcttcta cacaagcacg tggattcttc aaacgccaat
    14401 ctggtggtaa aggtcaattt ggtgatgttt ggattgaatt tacaccaaac gaagaaggta
    14461 aaggattcga attcgaaaat gctatcgtcg gtggtgtggt tcctcgtgaa tttattccag
    14521 ccgttgaaaa aggacttgta gaatctatgg caaacggtgt tcttgctgga tatccaatgg
    14581 ttgacgtaaa agctaaactt tacgatggtt cataccatga tgttgactca tctgagacag
    14641 ccttcaagat tgctgcatca cttgcactta aagaagctgc taaatcagcg caaccagcta
    14701 tccttgagcc aatgatgctt gttacaatta cagctccaga agacaatctt ggtgacgtta
    14761 tgggtcacgt gactgcacgt cgcggtcgtg ttgatggtat ggaagcacgt ggtaatacac
    14821 aagttgttcg tgcctttgtt ccacttgctg aaatgtttgg ttatgcaaca gttcttcgtt
    14881 ctgcaacaca aggacgtggc acattcatga tggtatttga ccactacgaa gatgttccta
    14941 agtcagttca agaagaaatc atcaagaaaa actctggtga ataatttctg aaaacttatg
    15001 aaaggtactc gaaagagtat ctttttttct ttttgaaagt ggtaaattca ttgcaatttt
    15061 tctcgaaaaa agatataata attgtgttga aagatttttg cacatatgta caagttaatc
    15121 ttttcacatt gattaggggg atccctaaaa taaaatattt gagattttca taaggaggaa
    15181 atcactaatg gtagttaaag ttggtattaa cggtttcggt cgtatcggtc gtcttgcatt
    15241 ccgtcgcatc caaaacgtag aaggtgttga agttactcgt atcaacgacc ttacagatcc
    15301 aaacatgctt gcacacttgt tgaaatatga cacaactcaa ggtcgtttcg acggtactgt
    15361 tgaagttaaa gaaggtggat tcgaagttaa cggtcaattt gttaaagttt ctgctgaacg
    15421 cgaaccagca aacattgact gggctactga tggcgtagaa atcgttcttg aagcaactgg
    15481 tttctttgca tcaaaagaaa aagctgaaca acacatccat gaaaatggtg ctaaaaaagt
    15541 tgttatcaca gctcctggtg gaaacgacgt taaaacagtt gttttcaaca ctaaccacga
    15601 tatccttgat ggaactgaaa cagttatctc aggtgcttca tgtactacaa actgtcttgc
    15661 tccaatggct aaagctttac aagacaactt tggtgttaaa caaggtttga tgactactat
    15721 ccacgcatac actggtgacc aaatgatcct tgacggacca caccgtggtg gtgaccttcg
    15781 tcgtgctcgt gcaggtgctg caaacatcgt tcctaactca actggtgctg caaaagctat
    15841 cggacttgtt atcccagaat tgaacggtaa acttgatggt gctgcacaac gtgttcctgt
    15901 tccaactgga tcagtaactg aattggttgc aactcttgaa aaagacgtaa ctgtcgaaga
    15961 agtaaatgca gctatgaaag cagcagctaa cgattcatac ggttatactg aagatccaat
    16021 cgtatcatct gatatcgttg gtatttcata cggttcattg tttgatgcta ctcaaactaa
    16081 agttcaaact gttgacggta accaattggt taaagttgtt tcatggtacg ataacgaaat
    16141 gtcatacact tcacaacttg ttcgtacact tgaatacttt gcaaaaattg caaaataatt
    16201 tttaattaat atga
//
LOCUS       DATROD010000034        15436 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.034, whole genome shotgun sequence.
ACCESSION   DATROD010000034 DATROD010000000
VERSION     DATROD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15436
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.034"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            159..1496
                     /locus_tag="VBC74_001030"
     CDS             159..1496
                     /locus_tag="VBC74_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEO6941992.1"
                     /translation="MADVKVVNNEGSRGQKQDLKTKLFHIKVGSVPLPVYVCLALLIL
                     LAGFLQKLPVNMLGGFAVILTMGWFLGTIGASIPGFKNFGGPAILSLLVPSILVFFNL
                     INKNVLESTNMLMKQANFLYFYIACLVSGSILGMNRKMLIQGLLRMIFPMLLGMVCAM
                     MVGTFVGVILGLEWRHTLFYIVTPVLAGGIGEGILPLSLGYSSITGVASEQLVAQLIP
                     ATIIGNFFAILCTALLNRLGEKKPHLSGQGQLVRLNKGEDMSDIIADHSGPIDVKKMG
                     GGVLTACSLFIFGHLLQQLTGFPGPVLMIIAAAILKYINVIPRETQNGAKQLYKFISG
                     NFTFPLMAGLGLLYIPLKDVVATLSIQYFIVVISVVFTVISVGFFVSRFLNMNPVEAG
                     IISACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRMLF"
     gene            1521..2684
                     /locus_tag="VBC74_001031"
     CDS             1521..2684
                     /locus_tag="VBC74_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEO6941993.1"
                     /translation="MSENLGQLAINQARENGGKLEVISKVKVEDKRDLSIAYTPGVAS
                     VSSAIAEDVELAYELTTKKNTVAVVSDGSAVLGLGDICPEAAMPVMEGKAALFKRFAN
                     VDAVPIVLKTNDTEEIISIVKAISPTFGGINLEDISAPRCFEIEQRLIEECDIPVFHD
                     DQHGTAIVVLAALFNSLKLVKKDIEDIKVVVNGGGSAGLSITRKLLSAGAKHVTVVDR
                     FGIINDKDRESLAPHHKAIAKLTNREFQSGSLEDALENADVFIGVSAPEALHAEWISK
                     MADKPIVFAMANPIPEIYPDQALKAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAARGIASLIPEEELSTTHIIPNAFQNDVADVVAKSVSNAVQK"
     gene            2875..3219
                     /locus_tag="VBC74_001032"
     CDS             2875..3219
                     /locus_tag="VBC74_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEO6941994.1"
                     /translation="MYSFEELLATMTLITAAEIEDKIDSNQDFVLFIGRISCPFCHLF
                     VPKIVEVADEDEFELFHLDSEDFDHWTANKDFRNKYDIPTVPGLMVVKNGTIKVKCDS
                     KMTKEEIREFIG"
     gene            3296..3637
                     /gene="yajC"
                     /locus_tag="VBC74_001033"
     CDS             3296..3637
                     /gene="yajC"
                     /locus_tag="VBC74_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEO6941995.1"
                     /translation="MSLPIIMLVVMVGMMFFMQRQQKKQAQERQKQLNAVQKGDEIVT
                     IGGLFGVVDEVNTEAQRMVLDVDGVYLTFELAAIKSVVSKAATPTEPVEDVKIIEVNE
                     DENKETAIETE"
     gene            3804..4556
                     /locus_tag="VBC74_001034"
     CDS             3804..4556
                     /locus_tag="VBC74_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEO6941996.1"
                     /translation="MFIFKRKKQVEMPLEKIPAHIGIIMDGNGRWAKKRLKPRVMGHK
                     AGMDALQEVTIAASELGVKVLTVYAFSTENWSRPDDEVKFIMNLPVEFFDKYVPELDK
                     NNVRVQVIGDTHKLPKATYDAMQRACLRTKHNSGLVLNFALNYGGRSEITNAIKEIAQ
                     DVLEAKLNPDDITEDLVANHLMTNSLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEF
                     YFTPVLWPDFKKDELHKAIVDYNQRHRRFGSV"
     gene            4571..5365
                     /locus_tag="VBC74_001035"
     CDS             4571..5365
                     /locus_tag="VBC74_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEO6941997.1"
                     /translation="MKERVIWGAVALAIFIPFLVMGGLPFQFLVGLLAMIGVSELLRM
                     RRLEIFSFEGALAMIGAFVLTVPLDSYLSFLPVDASLSAYGIVIFMILAGTVLNSNSY
                     SFEDAAFPIASSFYVGIGFQNLVSARMAGIDKVLLALFIVWATDIGAYMIGRQFGQRK
                     LLPSVSPNKTIEGSLGGIASAIVVAFFFMLFDKTVYAPHSFLVMLVLVAIFSIFGQFG
                     DLVESSIKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHFFGLF"
     gene            5396..6655
                     /gene="rseP"
                     /locus_tag="VBC74_001036"
     CDS             5396..6655
                     /gene="rseP"
                     /locus_tag="VBC74_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEO6941998.1"
                     /translation="MLGILTFIIIFGVIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHIDKEGTTYTIRILPLGGYVRMAGWGDDKTEIKTGTPASLTLNKEGIVTRINLSGK
                     QLDNTSLPINVTAYDLEDKLTITGLVLSETKTYSVDHDATIIEEDGTEIRIAPLDVQY
                     QNASVWGRLITNFAGPMNNFILGLVVFIALAFIQGGVQDLSTNQVRVSENGPAASAGL
                     KNNDRILQIGSHKVSNWEQLTAAVEKSTSHLEKNQKLALKIKSKEVVKTINVKPQKVD
                     KSYIIGIMPALKTSFKDKLLGGFKLAWESFFRILNELKKLIAHFSINKLGGPVALYQA
                     SSQAAKNGFVTVLNLMGLISINLGIMNLIPIPALDGGKIVMNILEAIRRKPLKQETET
                     YITLAGVAVMLVLMIAVTWNDIMRAFF"
     gene            6747..8600
                     /locus_tag="VBC74_001037"
     CDS             6747..8600
                     /locus_tag="VBC74_001037"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEO6941999.1"
                     /translation="MKQSKMLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMRQEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDQS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHKDYEDLDVTYEDYRKAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAVTPNRT
                     DLNRWLVLDKTIPSIDDIPEDVLEEIKAELSAWLVSGEDTIAYSTESSYAANLEMATN
                     EYKPSTKAATFEEVTKVETPNCKSIDEVAGFLSIDENQTIKTLLFIADEQPVVALLVG
                     NDQVNDVKLKNYLAADFLEPASEEQAKEIFGAGFGSLGPVNLPESVKIIADRKVQDLA
                     NAVSGANQDGYHFTGVNPERDFTAEYVDIREVKEGEISPDGKGTLKFARGIEIGHIFK
                     LGTRYSDSMGANILDENGRSNPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGAYRF
                     AWGINFPEELAPFDVHLITVNVKDQESQDLTEKIEADLMLKGYEVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKASEGIVEVKIKASGDTIEVHADNLIETLEILTKK"
     gene            8725..9309
                     /locus_tag="VBC74_001038"
     CDS             8725..9309
                     /locus_tag="VBC74_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEO6942000.1"
                     /translation="MKSRKKDKLVLRLTTTLLVFGLGGVWFYNYKNDNVEPTVTSASD
                     QTTTFIQTISPTAIEISKTYDLYASVLLAQAILESSSGQSDLSKAPNYNLFGIKGEYK
                     GKSVQMPTLEDDGKGNMTQIQAPFRAYPNYSASLYDYAELVSSQKYASVWKSNTSSYK
                     DATAALTGLYATDTAYASKLNQIIETYSLDAYDK"
     gene            9432..13838
                     /locus_tag="VBC74_001039"
     CDS             9432..13838
                     /locus_tag="VBC74_001039"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEO6942001.1"
                     /translation="MSELFKKLMDQIEMPLEIKNSSVFSSADIIEVKVHSLSRLWEFH
                     FSFPELLPIEVYCELQTRLVNSFEKADIKATFDIRAETIDFSDDLLQDYYQQAFCEPL
                     CNSASFKSSFSQLKVHYNGSQMIISAPQFVNNNHFRQNHLPRLEQQFSLFGFGKLAID
                     MVSDEQMTQNLKSSFETNREQLLEKANQEAMQALEAQKSLEDSAPPSEEVTPTQNYDF
                     KERIKQRQAGFEKAEITPMIEVTTEENRIVFEGMVFSVERKTTRTGRHIINFKMTDYT
                     SSFAMQKWAKDDEELKKYDMISKGSWLRVRGNIENNNFTKSLTMNVQDIKEIVHHERK
                     DLMPADQKRVEFHAHTNMSTMDALPTVESLIDTAAKWGHPAIAITDHANVQSFPHGYH
                     RAKKAGIKAIFGLEANIVEDKVPISYNEVDMNLHEATYVVFDVETTGLSAANNDLIQI
                     AASKMFKGNIIEQFDEFIDPGHPLSDFTTELTGITDNHVRGSKPILQVLQEFQNFCQG
                     TVLVAHNATFDVGFMNANYERHNLPLITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQ
                     VALEHHHMANYDAEATGRLLFIFLKEARENRDVTNLMELNTKLVAEDSYKKARIKHAT
                     IYVQNQVGLKNIFKLVSLSNVKYFEGVARIPRSVLDAHREGLLLGTACSDGEVFDALL
                     SNGIDAAVTLAKYYDFIEVMPPAIYRPLVVRDLIKDEAGIQQIIRDLIEVGRRLDKPV
                     LATGNVHYIEPEDEIYREIIVRSLGQGAMINRTIGRGEDAQPAPLPKAHFRTTNEMLD
                     EFAFLGKDLAYEIVVTNTNTFADRFEDVEVVKGDLYTPFVDRAEERVAELTYAKAFEI
                     YGNPLPDIIDLRIEKELASILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVA
                     TMIGITEVNPMPPHYVCPNCQHSEFITDGSCGSGYDLPNKNCPKCGTLYKKDGQDIPF
                     ETFLGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGEEYAFRAGTVGTVAEKTAFGFVK
                     GYERDYNKFYNDAEVERLATGAAGVKRSTGQHPGGIVVIPNYMDVYDFTPVQYPADDM
                     TAAWQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPSNILPDDPDVMKLFS
                     GTEVLGVTEEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWL
                     GNAQDLIKEGIATLSTVIGCRDDIMVYLMHAGLQPKMAFTIMERVRKGLWLKISEDER
                     NGYIQAMRDNNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHYPIFYYCAYF
                     SIRAKAFELRTMSAGLDAVKARMKDITEKRQRNEATNVENDLFTTLELVNEMLERGFK
                     FGKLDLYRSHATDFIIEEDTLIPPFVAMEGLGENVAKQIVRAREDGEFLSKTELRKRG
                     GVSSTLVEKFDEMGILGNLPEDNQLSLFDDFF"
     gene            13965..14390
                     /locus_tag="VBC74_001040"
     CDS             13965..14390
                     /locus_tag="VBC74_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6942002.1"
                     /translation="MGNFKNSAVKSMVVMRKAFRTIDGKVSESFKEFELTPTQFAVLD
                     VLYAKGTMKIGELIENMLATSGNMTVVIKNMEKKGWVLRHSCPNDKRAFLVSLTTEGE
                     EVIKKALPEHIKRVEDAFSVLTETEQEDLINLLKKFKTL"
     gene            14523..15137
                     /locus_tag="VBC74_001041"
     CDS             14523..15137
                     /locus_tag="VBC74_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEO6942003.1"
                     /translation="MSFLEELKNRRSIYALGRNTEVSDEKIVEIIKEAVRQSPSAFNS
                     QTSRVVILLNDEVTKFWDELVANDLVETMKVQGAPETAIAGTKEKLASFGASKGTVLF
                     FEDQDVVKSLQEQFVLYADNFPVWSEQSTGIASVNTWTALSAELGLGGNLQHYNPVID
                     ASVQAVYGVPASWKLRGQLNFGSIEAETGEKEFMNDDDRFKVIG"
BASE COUNT         4849 a         2420 c         3216 g         4951 t
ORIGIN      
        1 ttcatcagca tcctccgagt ttagtataac atagcctaaa aactagaatc cagcttaaat
       61 taattaaata agttgaaata attcaattac ttaaataaac ttccttatca ctaagtccca
      121 aagtaaactg tacataagaa gtaaaaggag gtgctttcat ggcagacgtg aaggtagtga
      181 ataatgaggg ttctagaggt caaaagcaag acttaaagac taaactattt catattaagg
      241 taggctcagt tcccttacca gtatatgttt gtttagcatt attgattctt ctagcaggct
      301 ttttacaaaa attgccagtc aatatgctag gaggatttgc agtcatctta acaatgggat
      361 ggttcttagg tactattgga gctagcatcc ctggttttaa aaacttcggt ggcccagcta
      421 ttttatcttt attagtacca tctattttgg tgtttttcaa cctcattaat aaaaatgttt
      481 tagagtcaac aaatatgttg atgaagcaag ctaactttct ttatttttat attgcttgtt
      541 tagtgtccgg tagtatttta gggatgaatc ggaaaatgtt gattcaggga ttactaagaa
      601 tgattttccc catgttatta ggaatggttt gtgcgatgat ggtagggaca tttgtcggtg
      661 ttattttagg cttagagtgg cgacacactt tgttttatat cgtaacaccc gttttagctg
      721 gtggtattgg tgaaggtatt ttaccattat cgttaggcta tagttcaatt accggtgtag
      781 ctagcgaaca actagttgct caactcatcc cagccactat tattggtaat ttctttgcca
      841 ttttatgtac tgcactattg aatcgtttgg gagaaaagaa accacacttg tctggtcaag
      901 ggcaattagt aaggttaaat aaaggagagg acatgtcaga tattattgct gatcattctg
      961 gcccaattga cgttaagaaa atgggtggag gtgttttaac agcatgtagt ctctttattt
     1021 ttggacattt gttgcagcaa ttaactggat ttcctggtcc cgtattaatg attatcgcag
     1081 cagctatttt gaaatatatc aatgttattc ctagagaaac acaaaatgga gctaagcaac
     1141 tttataaatt tatttctggt aattttacat ttcctctaat ggcagggcta ggattgcttt
     1201 atatcccgtt aaaagatgtt gtggcaacgc ttagcataca atatttcata gttgttatta
     1261 gtgttgtatt tacagttatt tctgttggat tctttgtatc gcgattcctt aatatgaatc
     1321 ctgttgaagc aggtattatt tcagcttgtc aaagtggtat gggaggaaca ggagatgttg
     1381 ccattttaag tacagcagac cgaatgaact tgatgccatt tgctcaagtt gctacgcgtt
     1441 taggaggagc tattactgtt atcacgatga cagccatttt acgcatgtta ttctaatatt
     1501 tatcaaaagg agttatcaaa atgagtgaaa atttaggtca attagctatc aaccaagcta
     1561 gagaaaatgg tggaaaatta gaagtaattt caaaagttaa ggtggaagat aagagggatc
     1621 ttagtattgc ctatacccca ggggtggcgt cagtatcatc ggctattgca gaggatgtgg
     1681 agcttgcata tgagttgacg actaaaaaaa atacagtagc cgtagtaagt gatggttcag
     1741 ctgttcttgg attaggagat atttgtcctg aggcagcaat gccagtcatg gaaggtaaag
     1801 cagcactctt taaacgtttt gcaaatgtag atgctgtgcc aattgtgttg aaaacaaatg
     1861 atacagagga aattatttct atagtaaaag cgatttcgcc gacttttgga gggattaatt
     1921 tagaggatat ttcagcacca cgttgtttcg aaattgaaca acgcctaatt gaagaatgtg
     1981 atataccagt tttccatgat gaccaacatg gtacagcaat cgtagtatta gcggctttgt
     2041 ttaacagttt gaagttagtt aaaaaagaca ttgaagatat caaagtagtt gttaatggtg
     2101 gaggttcagc gggcttatct ataacaagga agcttctatc agcaggagca aaacatgtaa
     2161 cggtagttga tcgatttggc attattaacg ataaagatcg ggagtcatta gcccctcatc
     2221 acaaagcgat tgcaaaattg actaatagag aattccaatc tggatcctta gaagatgctt
     2281 tggagaatgc cgatgttttt ataggtgttt ctgctccgga agcattacat gcagaatgga
     2341 tttccaagat ggctgataaa cctattgtat ttgcaatggc gaatcctatt ccagaaattt
     2401 atccagatca agccttaaaa gccggagctt acatagttgg aactggtcgt agtgattttc
     2461 caaatcaaat taataatgtt ttagcatttc cgggtatttt cagaggagca ttagatgcta
     2521 gagcaaaaac gataactgtt gaaatgcaaa ttgcggcagc gagaggcata gctagtttaa
     2581 taccggagga agaattaagc acaactcaca tcattccaaa tgcttttcaa aatgatgtgg
     2641 ccgatgtagt tgctaaaagc gttagtaatg ctgtacaaaa ataaggtgta aaataaaaac
     2701 tgagctatgc tcagtttttt tgatgagtca tttaaatatg agtgagcatt tttgaggata
     2761 gtttgaagcg agaagtgagt taatgatttt gatagaatcg ttgttactct tgccttgcaa
     2821 gttttgccat cataggttat tctttaaata taagatttag aaagttggtt aactatgtat
     2881 tcttttgaag aacttttggc aaccatgact ttgataacgg ctgccgaaat tgaagataaa
     2941 attgattcaa atcaagattt tgtactgttc attggtcgta tttcttgtcc attttgtcac
     3001 ctctttgttc ctaaaattgt agaagtagct gatgaagatg aatttgaatt attccattta
     3061 gatagtgaag attttgatca ttggactgca aataaagatt ttcgaaataa atatgacatt
     3121 cctacagtgc caggtttgat ggttgttaaa aacggaacta tcaaggtgaa gtgcgattca
     3181 aaaatgacaa aagaagaaat ccgtgaattt attggctaag agacagtatc tccttgcaga
     3241 tatagcataa atctgctata gtaatgatta cgtagaacac ggaaaggaga atcaaatgag
     3301 cttacctatt attatgcttg ttgttatggt tggtatgatg ttcttcatgc aacgtcagca
     3361 aaagaaacaa gcacaagaac gtcaaaaaca attgaatgct gttcaaaaag gtgatgaaat
     3421 tgtcacaatt ggtggtcttt ttggagtagt tgatgaagtt aatactgaag cacaacgaat
     3481 ggttttagat gttgatggtg tctacttgac gtttgagttg gcagctatca aatctgttgt
     3541 ctctaaggca gcaacaccaa cagaaccagt tgaagatgtt aagattattg aagtcaatga
     3601 agacgaaaat aaagaaacag ccatcgaaac tgaataagat atgtaggacc agatgattgt
     3661 tctggtcttt ttagtttgct gtcacaaatg gcattttaat actcaaaaat aagttaaaat
     3721 gtggtaaaat gttatagtat gggatatttc cagctctttt aaaagggctg ataagaaaaa
     3781 cgattgctaa attaaggaac attatgttta tatttaaaag aaaaaaacaa gtagaaatgc
     3841 ctttggagaa aataccagca catattggta ttatcatgga tggtaacgga cgttgggcaa
     3901 aaaaacgttt aaaaccacgc gttatgggac ataaggcggg gatggatgct cttcaagaag
     3961 ttacaatagc agcttcagaa ttaggtgtta aagtactaac tgtctatgcc ttttctacag
     4021 aaaattggtc acgtccagat gatgaagtta agtttattat gaaccttccg gttgaatttt
     4081 ttgataaata tgtaccagag ttagataaaa ataatgtacg tgtacaggtc atcggagata
     4141 ctcataaatt acctaaggca acatacgatg caatgcaacg cgcttgtttg aggacaaaac
     4201 ataattcggg gctcgtattg aattttgcgc taaattatgg cggacgttca gaaatcacta
     4261 atgccattaa ggaaattgct caggatgttt tggaagctaa gttgaatcct gacgatatta
     4321 cagaggattt agtagcaaat catttaatga caaatagctt accctatctt tatagggacc
     4381 ctgatttgat tatccgcact agtggagagt tacgcttgag taacttttta ccttggcaat
     4441 cagcttatag tgaattttac tttactcccg ttttatggcc agattttaaa aaagacgaac
     4501 tacataaggc cattgtggat tataatcaac gccatcgtcg atttggtagt gtatagaaag
     4561 aagagagaaa atgaaagaac gtgttatttg gggagctgtc gcgcttgcca tatttatccc
     4621 ttttttagta atgggtggtt tgcctttcca atttttagtt ggacttttag ctatgattgg
     4681 tgtttctgaa ttacttcgta tgcgtcgttt agaaatattt tcctttgaag gtgcattagc
     4741 tatgatagga gcctttgttt taacagttcc tttagatagt tatttgtcct ttttaccagt
     4801 tgacgctagt ttatcagcat acggtattgt tatttttatg attttggcag gtactgtttt
     4861 gaacagtaat tcttacagtt ttgaagatgc tgcctttcca attgcgtcta gtttttatgt
     4921 gggtattggc tttcaaaatc tagtcagtgc tcgtatggct ggaatagata aggtgttgtt
     4981 agctttattt attgtctggg cgactgatat cggtgcttat atgattggtc gtcaatttgg
     5041 acaacgcaaa ctactacctt cagtgtctcc aaataaaaca attgaaggga gtctgggagg
     5101 aattgcttca gccattgtgg tagctttctt ttttatgctt tttgataaga ccgtctatgc
     5161 gcctcatagc ttcctagtca tgttagtttt agtggctatc tttagcattt ttggtcaatt
     5221 tggagattta gttgaaagta gtattaaacg tcattttggt gttaaagatt cgggtaaatt
     5281 gattccgggt catgggggga ttttggatcg ctttgatagt atgatttttg ttttcccaat
     5341 catgcacttc tttggcttat tctaaaataa tatattgtat ttagagagga attgaatgct
     5401 aggaatttta acctttatta ttatttttgg tgtcattgta gttgttcacg aatttgggca
     5461 tttctatttt gcaaagaaat caggaattct tgttcgtgaa tttgctattg gtatgggtcc
     5521 aaaaatcttt tctcacattg ataaagaagg aacaacctat actattcgta tattaccgct
     5581 aggtggttat gtccgtatgg ctggttgggg tgatgataag actgaaatta agacagggac
     5641 gccggctagc cttactttaa ataaagaagg cattgtaact cgtattaatt tatcaggaaa
     5701 acaattagac aatacttctt taccaatcaa tgtaacagct tatgatttag aagataaact
     5761 aactatcaca ggattggtct taagtgagac aaaaacctat agtgttgatc acgatgctac
     5821 tatcattgag gaggatggta ctgaaattcg tattgctccc ttagatgtgc agtatcagaa
     5881 tgcttctgta tgggggcgct taattaccaa ttttgcaggt ccgatgaata attttatttt
     5941 aggtcttgta gtctttattg cacttgcttt tattcaagga ggtgttcaag atttatcaac
     6001 caatcaggtc cgtgtatcag aaaatggtcc tgctgctagt gcaggtttaa aaaataatga
     6061 ccgtatttta caaatagggt cgcacaaagt ttctaactgg gaacagttga cagcagcagt
     6121 agaaaagtca actagtcatt tagaaaaaaa ccaaaaactt gcccttaaaa ttaagagcaa
     6181 agaggtggtg aaaacgatca acgttaagcc acagaaagtt gataaatcct acatcatcgg
     6241 cattatgcca gctctaaaga caagctttaa agacaagctt ttaggtggat ttaaactagc
     6301 atgggaaagt ttctttagaa ttttaaatga attaaagaaa cttattgctc attttagtat
     6361 taataaattg ggaggtccag tagctctcta tcaggctagt agccaagctg ctaaaaatgg
     6421 tttcgtcaca gttctcaact taatgggatt aatctctatc aacttaggta ttatgaatct
     6481 catccctata ccagcccttg acgggggtaa gatagtgatg aatattttag aagccattcg
     6541 tcgcaaacca cttaaacaag aaacagaaac ttatattacg ctcgctgggg tagcagttat
     6601 gttagtatta atgattgctg taacctggaa cgatatcatg cgagctttct tctaaaagat
     6661 aataaaacgt gaaaggaaat acgaattttt caattgcaat tctcaattga aaaagaatca
     6721 actttgatta gtcgtatctt attgtaatga aacaaagtaa aatgcttatt ccaacacttc
     6781 gcgaaatgcc aagtgatgct caggttatca gccatgctct tatggttcgt gcaggatatg
     6841 tccgccaagt ttcggctgga atctatgctt atttgcctct agctaaccgt actattgaaa
     6901 aatttaaaac aattatgcgt caagaatttg agaagattgg tgcagttgaa atgttagctc
     6961 cagctcttct tacagcagat ttatggcgtg aatctgggcg ctacgaaaca tatggtgaag
     7021 acctttataa attaaagaat cgtgatcaat cggattttat cttaggacca actcatgaag
     7081 agactttcac aactcttgtt cgtgatgcag taaaatctta taagcaatta ccactaaacc
     7141 tttatcaaat tcagtctaaa tatcgtgatg agaagcgtcc tcgtaatgga cttcttcgaa
     7201 cacgtgaatt tatcatgaaa gatggataca gcttccacaa agattatgaa gaccttgatg
     7261 tcacttacga agattaccgt aaagcatatg aagccatctt cacacgtgct ggacttgact
     7321 ttaaaggcat tataggtgac ggaggtgcta tgggaggtaa agatagccaa gaatttatgg
     7381 cagtaactcc caatcgtaca gatttaaatc gttggttagt ccttgataaa actattcctt
     7441 ctattgatga tattccagag gatgttttag aagaaattaa ggcagaactc tcggcttggc
     7501 tagtttctgg tgaggacact attgcatact ccactgaatc aagctatgct gctaaccttg
     7561 agatggcaac gaatgagtac aaaccatcta ctaaggctgc tacctttgaa gaagtaacaa
     7621 aagtagaaac tcctaactgc aaatcaattg atgaggtagc tggattttta tcaattgatg
     7681 aaaatcaaac tatcaagaca ctattgttta ttgctgatga acagcccgtg gttgctttac
     7741 tagttggtaa cgatcaagtc aatgatgtga agttaaaaaa ttatttagcg gctgatttct
     7801 tagagcctgc aagtgaagag caagcaaaag agatttttgg tgcaggtttt ggttctcttg
     7861 ggccagttaa cctacctgag tctgttaaaa ttattgctga tcgtaaagtt caagaccttg
     7921 ctaatgctgt gtctggtgct aaccaagatg gttatcattt tactggagtt aatccagaac
     7981 gtgatttcac agctgaatac gttgatattc gtgaagtaaa agaaggggag atttctccag
     8041 acggtaaagg tactctgaaa tttgctcgtg gtattgaaat tggacatatc tttaaactag
     8101 gtacccgtta ttcagatagt atgggagcaa acatccttga tgaaaatggc cgctcaaatc
     8161 ctattgttat ggggtgttat ggtattggtg ttagtcgtat tctctcagct gtcattgaac
     8221 aacatgcccg actttttgtg aataagacac caaaaggagc ttatcgcttt gcttggggga
     8281 ttaatttccc agaagaatta gccccatttg atgtacattt gattactgtc aatgtcaaag
     8341 atcaagagtc acaagatctt actgagaaaa ttgaagctga tttgatgctt aaaggttatg
     8401 aagtcctgac agacgatcgt aatgaacgtg ttggttctaa attctcagac agtgacttaa
     8461 ttggacttcc aattcgagta actgttggga agaaagcttc tgaaggtatc gttgaagtga
     8521 aaattaaagc aagtggtgat acgattgagg ttcacgctga taacttaatt gaaactttag
     8581 agattctaac aaaaaaataa gccgataaaa aagttagtct gatgtgctaa cttttttata
     8641 tggagtgaaa tttagtatgg ttcttattgg taaaaagcaa ctgccttttg ttataatgaa
     8701 atgatatttt agaaaggagc catcatgaaa agtcgaaaaa aagataaatt ggtattgagg
     8761 ttaacaacaa cactattagt ttttggtttg ggtggggttt ggttttacaa ttataaaaat
     8821 gataatgtcg aaccgacagt cactagtgca tcggatcaaa cgacgacttt tattcaaacg
     8881 atttctccaa cagctattga aatttctaag acctatgatt tgtatgcgtc agtcttatta
     8941 gcacaagcta ttttggaatc atccagtgga caatcagatt tgtctaaggc tcctaattat
     9001 aacctctttg gcatcaaagg agaatataaa ggtaaatctg tccaaatgcc tactttagaa
     9061 gatgatggga aaggcaatat gactcaaatc caagctcctt ttcgcgccta tccaaattat
     9121 tctgcttcac tatatgatta tgctgagtta gtatctagtc aaaagtatgc atctgtttgg
     9181 aaatcaaata cttcttctta taaggatgct actgcagctc taacaggtct ttatgcgaca
     9241 gatactgctt atgctagtaa attaaaccaa attattgaaa cctacagtct agatgcttat
     9301 gataaatagt ctcagttaag agagtgttgt taagaaggct aataaatgcc ttttttaaca
     9361 gcctcctttt gttatctcga aatatttaca gatatgtgat aaaatggaag taagatattc
     9421 aggagaaaac tatgtcagaa ttgttcaaga aacttatgga tcagattgaa atgccgcttg
     9481 aaattaagaa ttcaagtgtt ttttcgtctg ctgatattat cgaggtaaag gttcattctc
     9541 tatcacgctt atgggaattt catttttcat ttccagaatt attgccaatt gaggtgtatt
     9601 gtgaattaca aactcgttta gtgaatagtt ttgaaaaagc agatataaag gctacttttg
     9661 atataagagc agaaactatt gatttttctg atgatctgct acaggattat tatcaacaag
     9721 ctttttgtga acccttgtgt aatagtgcaa gttttaagag ttctttttca caattaaaag
     9781 ttcattacaa tggcagtcag atgattattt cagcacctca atttgtcaat aataatcatt
     9841 ttcgtcagaa tcatttgccg cgcttggaac aacaattttc attgtttgga tttggtaaac
     9901 ttgctattga tatggtttct gatgaacaaa tgacgcaaaa cttaaaatca tcatttgaaa
     9961 ctaaccgcga gcaattgctt gaaaaagcta accaagaggc tatgcaagcc ttggaagcac
    10021 aaaaatcact agaagattca gcgcctccat cagaagaagt gacaccaact caaaattatg
    10081 atttcaaaga gcgtatcaaa caacgtcaag ctggctttga aaaagcagag attacaccca
    10141 tgattgaggt gaccacagaa gaaaatcgta tcgtttttga aggaatggtt tttagtgttg
    10201 aacgaaaaac aacccgcact ggacgtcata tcattaattt taagatgact gattatacct
    10261 caagttttgc tatgcaaaag tgggctaaag atgatgaaga attgaagaag tacgatatga
    10321 tttctaaagg ttcgtggttg cgggtcagag ggaatattga gaacaataac tttactaaat
    10381 ctttgacaat gaatgttcaa gatatcaaag agattgtgca tcatgaacgt aaagacttaa
    10441 tgccagcaga tcaaaaacgt gtggaatttc atgctcatac caatatgtca acgatggatg
    10501 ctttaccaac tgtcgagagc ctcattgata ctgcagctaa gtggggacac ccagctattg
    10561 caattactga ccatgccaat gttcaaagtt ttccgcacgg ctaccacaga gcaaaaaaag
    10621 caggtataaa agcaattttt ggattagagg caaatattgt tgaagataaa gtgcctattt
    10681 cctacaacga agttgatatg aatttgcacg aagcaactta tgttgtattt gatgtggaaa
    10741 ctacaggtct ttctgcagct aataatgacc taattcagat tgcagcatca aaaatgttca
    10801 agggtaatat tattgagcaa tttgatgagt ttattgaccc aggccatcct ctttctgact
    10861 ttacaactga gttgacaggt attacggata atcatgtcag aggttcaaaa cctattttac
    10921 aagtccttca ggaatttcag aatttttgtc aaggtacggt tttagttgcg cataatgcaa
    10981 cctttgacgt tggttttatg aatgcgaatt atgaacgtca taatctgcct ttgatcacac
    11041 agccggtcat tgatacctta gaatttgcaa gaaatcttta tcctgaatat aagcgtcatg
    11101 gtttggggcc tctaaccaaa cgtttccaag ttgctttgga acatcatcat atggccaact
    11161 acgatgctga agcaacgggt cgcttactct ttattttctt aaaagaggcg cgggaaaatc
    11221 gtgatgtgac taatttgatg gagctcaata ctaaattggt agctgaagac tcttacaaaa
    11281 aggcacgcat aaagcatgca accatttatg tccaaaatca ggttggactt aaaaatatat
    11341 ttaagttagt tagcctttca aatgttaaat attttgaagg ggtggcaaga attcctagat
    11401 cagttttaga tgctcacaga gaaggtctct tgctaggtac tgcctgttct gatggagaag
    11461 tttttgatgc cttgttatca aatggaattg atgcagcagt gaccctagct aaatattatg
    11521 attttattga agtcatgcca cctgctattt atcgcccctt ggtggttcgt gatttaatta
    11581 aagatgaggc ggggattcaa caaattatta gagacctaat agaggtcggt cggcgtttag
    11641 ataagcctgt tttagctacg ggaaatgttc attatattga gcctgaagat gagatttacc
    11701 gtgagattat tgttcgtagt cttggacaag gtgccatgat taataggact atcggtcgtg
    11761 gggaggatgc tcaaccagcg cctttgccaa aagcacattt ccgtacaaca aatgagatgt
    11821 tagatgaatt tgcctttctt ggtaaagatt tagcctatga gatcgttgta accaatacga
    11881 atacttttgc agatcgattt gaagacgttg aagttgtaaa aggggatctc tataccccgt
    11941 ttgttgatcg tgctgaagaa cgagttgctg aattaactta tgctaaagca ttcgaaatat
    12001 atggtaatcc tcttcctgat attattgatt tacgtatcga aaaagagtta gcatctatac
    12061 ttggtaatgg atttgccgtt atctatcttg cttcgcaaat gttggtgcaa cgttctaatg
    12121 aacgtggtta tctagtagga tcacgtgggt ctgttgggtc gagttttgta gcgaccatga
    12181 taggaattac tgaagttaat ccaatgccac ctcattatgt ttgcccaaat tgtcagcatt
    12241 ctgaatttat cacagatggt tcttgtggtt caggttacga tttaccaaat aaaaattgtc
    12301 ctaaatgtgg tactttgtac aagaaagatg gacaggatat tccgtttgaa actttcttag
    12361 gatttgatgg agataaggtt cctgatattg atctaaattt ctctggagat gatcaacctt
    12421 ctgctcactt agatgttcgt gatatctttg gagaagaata tgcttttcgt gcaggaacgg
    12481 taggtaccgt agctgaaaaa actgcctttg gttttgtcaa aggttacgaa cgagattaca
    12541 ataaatttta taacgatgca gaggtcgaac ggttagcgac tggtgctgcg ggagtcaaac
    12601 gttctacagg tcaacacccc ggtggtatcg ttgttattcc taactatatg gatgtttatg
    12661 attttactcc ggttcaatat ccagcagatg atatgacagc tgcttggcag acaactcact
    12721 tcaatttcca tgatattgat gagaatgtct tgaagttaga tattctagga catgatgatc
    12781 ctactatgat tcgtaaactt caggatttat caggaattga tccatcaaat atccttccag
    12841 atgatccaga cgttatgaag ttattttctg gtacggaagt tttaggagtg acagaagagc
    12901 aaattggaac tcctacaggg atgcttggta ttcctgagtt tggtacgaac tttgttcgtg
    12961 gtatggtgaa cgaaacccat ccaaccacat ttgcagaatt gttgcaactg tcaggactat
    13021 cacatggtac ggatgtttgg ttaggtaatg ctcaagactt aatcaaagaa ggtattgcta
    13081 ccttatcaac cgttattggt tgtcgtgatg acatcatggt ttatttaatg cacgctggtc
    13141 tacaaccaaa gatggccttt acaatcatgg agcgcgtgcg taaggggtta tggttgaaaa
    13201 tttcagaaga tgagcgtaac ggttatatcc aagcaatgcg tgataataat gtcccggatt
    13261 ggtatattga atcgtgtgga aaaatcaagt atatgttccc taaagcccat gcagcagcct
    13321 atgttttaat ggccttacgt gtggcgtact ttaaagtaca ttatccaata ttttattatt
    13381 gtgcttattt ttctattcgc gccaaagctt ttgaattacg cacaatgagt gctggacttg
    13441 atgccgttaa ggcgcgcatg aaggatatca ctgagaagcg tcagcgtaat gaggcgacga
    13501 atgtcgaaaa tgatcttttt acgacattag agttggttaa tgaaatgctt gagcgtggct
    13561 ttaaatttgg gaaattagat ctttatcgta gccatgcgac tgactttatt attgaagagg
    13621 atacgctaat ccctccattt gttgctatgg aaggcttggg agaaaacgtt gcaaaacaga
    13681 tagttcgagc acgtgaagat ggtgaatttt tatcaaaaac agagcttcgt aaacgaggag
    13741 gagtctcaag taccttagtt gaaaaatttg atgagatggg tattttggga aatttaccag
    13801 aagacaatca gttgagtctt tttgatgatt tcttctaagc aataaggttg agtagttact
    13861 caaccttatt atttgccagc tgcttttgat agggcaaaga tgatattctt gcttaaaata
    13921 actaattagt gataaaataa gaaaaaatac gtttgggaga tgaaatggga aactttaaaa
    13981 attcagcggt taaatctatg gttgtcatgc gaaaagcatt tagaacaata gacggaaaag
    14041 tctcagaatc atttaaagaa tttgaattaa caccaactca gtttgcagtt ttggatgttc
    14101 tctatgctaa aggcacaatg aaaattggag aattgattga aaatatgctt gctacatcag
    14161 gtaatatgac tgttgttatt aaaaatatgg aaaagaaggg atgggtatta cgccattctt
    14221 gtccgaatga taagagagcc tttttggtga gtttaacaac tgaaggagaa gaagtgataa
    14281 aaaaagctct accagagcat attaaacgtg tagaagatgc tttttcagtc ttaacagaaa
    14341 cagagcaaga agacttgatt aaccttttaa aaaaatttaa aaccttgtga gtcacattct
    14401 ttgactttac gatatagtgg taatataatt actacatagt aataaataac tttaatctaa
    14461 ttcatatgtc actagaacaa atgtagtaag atatctgaga ttgaagaaca aggagaaaaa
    14521 atatgtcatt tttggaagaa ttaaaaaacc gtcgctctat ttacgctctt ggtcgcaata
    14581 cagaagtttc tgatgagaaa atagtagaga ttattaaaga ggcagttcgc caatccccat
    14641 ctgcttttaa tagtcaaact tcccgtgttg tcattttatt gaatgatgaa gttactaagt
    14701 tctgggatga attggttgct aatgatttgg tagaaacaat gaaagtacaa ggtgctccag
    14761 agacagctat tgctggaact aaggaaaaat tagctagttt tggagcttca aagggaacag
    14821 tactcttctt tgaagatcaa gatgttgtca aatctcttca agaacaattc gttctttatg
    14881 cggataactt tccagtttgg tctgagcaat caacaggtat tgcctcagta aatacttgga
    14941 cagccctttc tgcagaactt ggacttggtg gtaatttaca acattacaat ccagttattg
    15001 atgcatcagt acaagctgtt tatggtgttc cagctagttg gaaacttcgt ggacaactaa
    15061 attttggttc aatcgaagct gaaacaggag aaaaagaatt catgaatgat gatgaccgct
    15121 ttaaagtcat cggataatac tgtacaaaaa aaggcaagga tagcaatggc actgcatcta
    15181 tgaactaaaa tgaaaaaaac actcctactt ctagtaaaat agaggtagga gtgtttttag
    15241 tatattcaaa aaagctacaa ttcgcttgat acggagactt ctgttatttg atgtagatat
    15301 ttgaaaggtt aattcgtgtt cgtttctcct ttttcgggat tttcacttag tttcatttct
    15361 ccttctttta attgtgaata gaatgtttca ttcaaattta gactttttgt aaactctgtg
    15421 tactcatgct tgaata
//
LOCUS       DATROD010000035        15413 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.035, whole genome shotgun sequence.
ACCESSION   DATROD010000035 DATROD010000000
VERSION     DATROD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15413
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.035"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            3..239
                     /locus_tag="VBC74_001042"
     CDS             3..239
                     /locus_tag="VBC74_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEO6942004.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGDAENYVQLFDTLEVNGKVLQAT
                     PYFLINVTGEGEGFSMWAPTPCDVLAEDWIEVND"
     gene            232..930
                     /locus_tag="VBC74_001043"
     CDS             232..930
                     /locus_tag="VBC74_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEO6942005.1"
                     /translation="MTKVVLVTGCASGIGYAQAQYFLKQGYQVYGVDKSDKPNLNGNF
                     NFIKLDLSSDLSPLFTMVPTVDILCNTAGILDAYKPLLEVSDEELEHLFDINFFVTVR
                     LTRHYLRRMVEKKSGIIINMCSIASFIAGGGGAAYTSSKHALAGFTRQLALDYAKDCI
                     QIFGIAPGAVQTAMTASDFEPGGLAEWVASETPIGRWTKPSEVAELTGFLASGKARSM
                     QGEIVKIDGGWSLK"
     gene            927..1172
                     /locus_tag="VBC74_001044"
                     /pseudo
     CDS             927..1172
                     /locus_tag="VBC74_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233822.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3977 family protein"
     gene            1169..1621
                     /locus_tag="VBC74_001045"
     CDS             1169..1621
                     /locus_tag="VBC74_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEO6942006.1"
                     /translation="MKAQELWNKFKSLNPSIGDEIDAWQFGAEPDLLAKLVVDGTKTA
                     TASAYDLYELDEEGLPQVGSYDIILDSNDEAICIIEITKVDIVPFKDVSADHAFKEGE
                     GDKTLEWWRKAHIDFFKPYFEEFGLMFSEDSRIVLEEFQVVYPKEINE"
     gene            1618..2574
                     /locus_tag="VBC74_001046"
     CDS             1618..2574
                     /locus_tag="VBC74_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEO6942007.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIEGIDLVAILSTVRSLETAKDLA
                     KEYNMSLATSEYKAVLDNEEIDTVYIGLPNHLHFDYAKEALLAGKHVICEKPFTLEAS
                     QLEELVSIANTRQLILLEAITNQYLPNFDLVKEHLSNLGDIKIVECNYSQYSSRYDAF
                     KRGEIAPAFNPEMGGGALRDLNIYNLHLVIGLFGEPITAQYLPNIERGIDTSGVLVLD
                     YGHFKTVCIGAKDCSAEVKSTIQGDKGSIAILGPTNTMPKISLTMNGQESHVYQLNGD
                     RHRMHDEFVIFEGIISNLDFKRAAQALEHSRTVMKVLDMVTK"
     gene            2663..3040
                     /locus_tag="VBC74_001047"
                     /pseudo
     CDS             2663..3040
                     /locus_tag="VBC74_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625774.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            3053..3466
                     /locus_tag="VBC74_001048"
     CDS             3053..3466
                     /locus_tag="VBC74_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6942008.1"
                     /translation="MVKALETYIVTNGNGRQAVDFYKDVFQADLVNMMTWEEMDPNCL
                     EDRKDLIINAQLIFDGIRLQISDENPDFVYQAGKNVTAAIIVGSVEEAREIYEKLKKS
                     AQEVQLELQETFWNPAYANLVDQFGVMWQISTELN"
     gene            3710..5089
                     /gene="glmU"
                     /locus_tag="VBC74_001049"
     CDS             3710..5089
                     /gene="glmU"
                     /locus_tag="VBC74_001049"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEO6942009.1"
                     /translation="MSNYAIILAAGKGTRMKSDLPKVMHKVSGITMLEHVFRSVQAIE
                     PSKIVTVIGHKAELVRDVLGDKSEFVMQTEQLGTGHAVMMAEEELATSKGHTLVIAGD
                     TPLITGESLKNLIDFHVNHKNVATILTADAANPFGYGRIIRNSDDEVTKIVEQKDAND
                     FEQQVKEINTGTYVFDNQSLFEALKDINTNNAQGEYYLTDVIGIFKEAGKKVGAYKLR
                     DFDESLGVNDRVALATAEKVMRHRIARQHMVNGVTVVNPDSAYIDIDVEIGEESVIEP
                     NVTLKGQTKIGKGTLLTNGSYLVDAQVGNDVTITNSMVEESIISDGVTVGPYAHIRPG
                     TSLAKGVHIGNFVEVKGSQIGENTKAGHLTYIGNAEVGCDVNFGAGTITVNYDGQNKF
                     KTEIGSNVFIGSNSTLIAPLEIGDNALTAAGSTITDNVPIDSIAIGRGRQVNKEGYAN
                     KKPHHPSQK"
     gene            5110..5664
                     /locus_tag="VBC74_001050"
     CDS             5110..5664
                     /locus_tag="VBC74_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6942010.1"
                     /translation="MDFEEKTINRQTVFDGQIIKVAVDDVELPNGLGQSKRELVFHGG
                     AVATLAVTPEHKIVLVKQYRKAIEGISYEIPAGKLETGESGSKEEAALRELEEETGYT
                     GNLEILYSFYTAIGFCNEKIVLYLATDLQKVENPRPQDDDEVLELLELSYEDCMQMVE
                     KGMIQDAKTIIALQYYGLKMGGHS"
     gene            5664..5933
                     /locus_tag="VBC74_001051"
     CDS             5664..5933
                     /locus_tag="VBC74_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942011.1"
                     /translation="MGKPLLTDDMIERSNRGEKVSGQTILDQETKIISTEDGMEQLTD
                     ENGKHIYKSRRIENAKRNEFQRKLNLVLFILLILLALLFYAIFKL"
     gene            5943..6632
                     /locus_tag="VBC74_001052"
     CDS             5943..6632
                     /locus_tag="VBC74_001052"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEO6942012.1"
                     /translation="MKIGIIAAMEEELKLLVENLEDKSQETVLSNVYYSGRYGEHELV
                     LVQSGVGKVMSAMSVAILVESFKVDAIINTGSAGAVATGLNVGDVVVADTLVYHDVDL
                     TAFGYDYGQMSMQPLYFHSDKTFVSTFEAVLSKEEMTSKVGLIATGDSFIAGQEKIDV
                     IKGHFPQVLAVEMEGAAIAQAAQATGKPFVVVRAMSDTAAHDANITFDEFIIEAGKRS
                     AQVLMAFLKAL"
     gene            complement(6672..7319)
                     /locus_tag="VBC74_001053"
     CDS             complement(6672..7319)
                     /locus_tag="VBC74_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEO6942013.1"
                     /translation="MTPNKEDYLKCIHELGEKNNKISNKKIAEMMQVSAPAVSEMIKK
                     MISEQLIVKDKDLGYYLTKQGLLVVSDLYRKHRLVEVFLVNHLHYTADDIHEEAEVLE
                     HTVSTTFVDQLEKLLDFPQFCPHGGTIPKKGEFLVEINQMTLDQISQLGTYVISRVHD
                     DFQLLKYLEQHRLHINDTIELTQIDPYAKTYHITYNDENLTIPERIASQIYVSAT"
     gene            7486..8412
                     /locus_tag="VBC74_001054"
     CDS             7486..8412
                     /locus_tag="VBC74_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEO6942014.1"
                     /translation="MKKWLVIVSCFVAFLGLGACANKQDQAKNEKLKVVVTNSILADI
                     TKNIAGNKIDLHSIVPVGKDPHEYEPLPADVEKTTQADLVFYNGINLETGGNAWFTKL
                     IKNAKKKENKDYFAVSDGVDVIYLNGQSGKGKEDPHAWLNLENGIIYSKNIAKQLIAK
                     DPKNKATYEKNRDAYVAKLEKLDKEAKSKFNAIPANKKLIVTSEGCFKYFSKAYGVPS
                     AYIWEINTEEEGTPDQITSLVKKLKQVRPSALFVESSVDKRPMKSVSRESGIPIYAEI
                     FTDSIAKKGQKGDSYYAMMKWNLDKIAEGLAK"
     gene            8583..9299
                     /locus_tag="VBC74_001055"
     CDS             8583..9299
                     /locus_tag="VBC74_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO6942015.1"
                     /translation="MIISKHLSVSYDNNLVLEDINLRLEGSGIIGILGPNGAGKSTLM
                     KALLGLVDSTGESGIGGDLLPLMGRVAYVEQKTNIDYQFPITVGECVSLGLYKERGLF
                     KRLSKTDWEKVSRVIDQVGLRGFENRPINALSGGQFQRMLMARCLVQEADYIFLDEPF
                     VGIDSISEQIIVNLLKKLSKAGKLILVVHHDLSKVDHYFDQVIILNRHLIACGPIDQA
                     FTRENLSAAYGDAILLGQGD"
     gene            9301..10134
                     /locus_tag="VBC74_001056"
     CDS             9301..10134
                     /locus_tag="VBC74_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO6942016.1"
                     /translation="MFTKFFEGLLTYHFLQNAFITAIVIGIVAGAVGCFIILRSMSLM
                     GDAISHAVLPGVAISFILGINFFIGAIVFGLLSSIIITYIKENSVIKGDTAIGITFSS
                     FLALGIILIGLANSTTDLFHILFGNILAVQDSDKYMTIIVGLIVLTLITIFFKELLLT
                     SFDPVLAKSMGMRVSFYHYLLMILLTLVAVTAMQSVGTILIVALLITPAATAYLYVKS
                     LRTMLFLSSALGAVASVLGLYIGYTFNIAAGSSIVLTSTFMFLLAFLFSPKQSLFKKK
                     Q"
     gene            complement(10186..10989)
                     /locus_tag="VBC74_001057"
     CDS             complement(10186..10989)
                     /locus_tag="VBC74_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEO6942017.1"
                     /translation="MKKIIYLGLACVSILTLSGCESIERSLKGDRYVDQKLAENSSKE
                     ATEQLNKKTKQALKADKKAFPQLDKAVAKNEAQVLIKTSKGDINIKLFPKYAPLAVEN
                     FLTHAKEGYYNGLSFHRVIKDFMIQSGDPNGDGTGGKSIWNSKDKKKDSGNGFVNEIS
                     PYLYNIRGSLAMANAGADTNGSQFFINQSQQDHSKQLSDKKVPKVIIKAYSEGGNPSL
                     DGGYTVFGQVISGMETVDKIASVEVTKSDQPKEKITITSIKVIKDYKFK"
     gene            11155..13605
                     /locus_tag="VBC74_001058"
     CDS             11155..13605
                     /locus_tag="VBC74_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO6942018.1"
                     /translation="MVFMANKKKTKGKKTRRPTKAEIERQRAIQRMITALVLTIILFF
                     GIIRLGIFGITVYNVIRFMVGSLAYLFIAATLIYLYFFKWLRKKDSLVAGFLIASLGL
                     LIEWHAYLFSMPILKDKEILRSTARLIVSDLMQFKITVFAGGGMLGALIYKPIAFLFS
                     NIGAYMIGVLFIILGLFLMSSLEVYDIVEFIRAFKNKVAEKHEQNKKERFAKREMKKA
                     IAEQERIERQKAEEEAYLASVNVDPETGEILEDQAEDNLDDALPPEVSETSTPVFEPE
                     ILAYETSPQNDPLPVEPTIYLEDYDSPIPNMRENDEEMVYDLDDDVDDSDIENVDFTP
                     KTTLVYKLPTIDLFAPDKPKNQSKEKDLVRKNIRVLEETFRSFGIDVKVERAEIGPSV
                     TKYEIKPAVGVRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSF
                     RELWEQSDANPENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISS
                     ILMKARPDQVKFMMIDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYE
                     LFSKIGVRNIAGYNTKVEEFNASSEQKQIPLPLIVVIVDELADLMMVASKEVEDAIIR
                     LGQKARAAGIHMILATQRPSVDVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKL
                     LGRGDMLFKPIDENHPVRLQGSFISDDDVERIVGFIKDQAEADYDDAFDPGEVSETDN
                     GSGGGGGVPESDPLFEEAKGLVLETQKASASMIQRRLSVGFNRATRLMEELEAAGVIG
                     PAEGTKPRKVLMTPTPSE"
     gene            13687..15402
                     /gene="pabB"
                     /locus_tag="VBC74_001059"
     CDS             13687..15402
                     /gene="pabB"
                     /locus_tag="VBC74_001059"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEO6942019.1"
                     /translation="MHIETVIDFKELGKRYRFKNPTKELIADTLEQVLEVIKEVDYYQ
                     SQNYYVVGYLSYEASAAFDSHFKVSQQKLAGEHLAYFTVHKDCENEAFPLSYENVRLA
                     DNWTANVSEQEYQEAIANIKGQIRQGNTYQVNYTLELSQQLCSDPFSVYERLMVEQGA
                     GYNAYIAYDDKRILSVSPELFFKKKDEVLTTRPMKGTSARKPTYQEDVAERDWLANDP
                     KNRSENMMIVDLLRNDMGRICDVGTVKVKKLCQVEQYATVWQMTSTIEGVLSPEVTLM
                     SIFQALYPCGSITGAPKISTMAIINELEKRPRGIYCGTIGLCMPDGQAIFNVPIRTVQ
                     MKGQQAYYGVGGGITWESQTDSEYEETRQKSAVLTRVNPKFQLITTGRVTENKLLFSQ
                     QHVERLVESASYFAYSFDKSKFERELKKYLHQLDEKDYRLKIMLDKTGKVTFEVKQLV
                     NLSKKFLTAEVVVQDYPIKLSPFTYFKTSYRPHIIEGQNEKIFVSPEGLLLETSIGNI
                     VLEKNGRFLTPDLSEGGLNGIYRRHLLKNQKVIEAPLTLKDLESADAIYACNAVRGLY
                     PLNLK"
BASE COUNT         5046 a         2242 c         3171 g         4954 t
ORIGIN      
        1 atatgacatt tgaagaaatt ttaccaggtt taaaagcaaa gaaaaaatat gtacgtacag
       61 gttgggggga cgctgaaaat tatgttcagt tatttgatac ccttgaagtt aatgggaaag
      121 tactacaggc tacaccttat tttcttatta atgttactgg tgaaggtgaa ggtttttcca
      181 tgtgggcacc aacaccatgt gatgttttag cagaggattg gatcgaggtc aatgactaaa
      241 gttgttttag tgacgggctg tgctagtgga attggttatg cacaggctca atattttttg
      301 aaacaagggt atcaagtata tggtgtggat aagtcggata aaccgaactt aaatggtaat
      361 tttaacttta taaagttgga tttatcctct gatttatccc cactattcac aatggtaccg
      421 actgttgata ttttgtgtaa tactgcaggt attctagatg cctacaagcc acttctagaa
      481 gtaagtgatg aagaactgga acacctattt gatattaatt ttttcgtgac tgttaggcta
      541 acacgtcatt atcttagacg tatggtggaa aagaagtcag gtattattat caatatgtgc
      601 tcaatagcaa gttttatagc gggtggcggt ggagctgcat atactagttc taaacatgcc
      661 cttgcgggat tcacacgtca attggctttg gattacgcaa aagattgtat tcaaattttt
      721 ggtatagcgc caggtgctgt acaaactgct atgactgcta gtgattttga acctggtgga
      781 ttagcagaat gggtagctag tgagacgcca attggtcgtt ggactaaacc aagtgaagtt
      841 gctgaattaa ctggatttct tgcttcagga aaagcgagat caatgcaagg tgaaattgtc
      901 aaaattgatg gtggctggag tttgaaatga gaaaatagat tgaatttggt tgtggtaata
      961 cttggttatt acgtacggaa tttgaagcat ctgatggtac tgagcgggaa gttaaaggaa
     1021 tatcaggtga tattttaata aaatctgttt atttacgtgt ttggattggt caaactgttt
     1081 atattttaga tgttaaagaa ggatttaaaa gacaaagaaa aatgagtaac gcagttaagt
     1141 ttgttgtggg aattgcttcg gaggtaatat gaaggcacaa gaattatgga ataagtttaa
     1201 atctttgaat ccttcaatcg gtgatgagat agacgcatgg caatttggag cagaaccaga
     1261 tcttctggca aagttagttg tagatgggac aaaaactgca acggcctctg cctatgattt
     1321 atatgaatta gatgaggaag ggttaccaca agttggttca tatgatatta ttttggatag
     1381 caatgatgaa gctatctgta ttattgaaat aactaaagtt gatattgtcc cttttaaaga
     1441 tgtctctgca gaccatgctt ttaaagaagg agaaggagat aaaactttag aatggtggag
     1501 aaaagctcac attgatttct ttaaacctta ttttgaggag tttggtttaa tgtttagtga
     1561 ggactctagg atagttttag aggaattcca agtggtttac ccaaaggaga ttaacgaatg
     1621 aaattagcgg ttttggggac aggtatgatt gttaaggaag ttttgcctgt cttgcagaaa
     1681 attgaaggta ttgatctagt tgctatctta tcaacagtta gaagtttgga gacagctaaa
     1741 gatctagcta aggaatataa catgtctcta gctacgagtg aatataaggc tgtcttggac
     1801 aatgaggaga ttgacacagt ctatattgga ctgccgaatc acttgcattt tgactatgct
     1861 aaggaggcac tcttggcagg aaaacatgtc atttgtgaga agccatttac attagaagca
     1921 agtcaattag aagaacttgt atctattgcc aatactaggc aactaatatt actcgaagca
     1981 attacaaatc aatatttacc taattttgac cttgtaaaag aacatttaag caaccttgga
     2041 gatattaaaa ttgtagaatg taattattcc caatattcat cgcgttatga tgcctttaaa
     2101 cgtggggaaa tagcaccagc tttcaatcct gaaatgggtg gaggtgcgtt gagagacctt
     2161 aatatttata atttacattt agtaattgga ctttttggag aacctataac agcacagtat
     2221 ttaccaaata tagagcgtgg tattgatact tcaggagttc tagtcctaga ctatgggcac
     2281 tttaaaacag tctgtattgg agcaaaagat tgtagtgcag aagtaaaatc tactattcaa
     2341 ggtgataagg ggtctatagc gattttaggg ccaactaata ctatgcctaa aatttcatta
     2401 actatgaatg gacaagaaag tcatgtttat caattaaatg gtgataggca ccgcatgcac
     2461 gatgaatttg ttatttttga aggtattatt agcaatttag attttaagcg tgctgctcaa
     2521 gctttagaac atagtagaac ggtgatgaaa gttctagata tggtaacgaa ataagtaaat
     2581 gactcttttg agtcattttt tacttatttt aaatactgta tgttatacta attagtaata
     2641 aaaccgaaag tgagataata aaatgatttc atcaattgga caggtaatgc tctacgttag
     2701 caatgttgaa gcatcagcag atttttggaa aaacaaggtg ggttttgagc gtgttgaaaa
     2761 atagacacaa ggagattatg taacctatat agttgcaccg aagttggata gtgaagttag
     2821 cttcgtctta catgacaaag caattatagc tcaaatgagt cctgagctag atttagcaac
     2881 tccctctatt ttatttgaaa ctactgatat tgacagtaca tatcaagaat taacagcaaa
     2941 tgaggttatg acaaatccta ttgttgatat ggggagtatg agagttttta atttttccga
     3001 taatgacaat aattactttg caatccgtga agtaaaataa aaaggagaac ttatggttaa
     3061 agcactagaa acatacatcg taacaaatgg aaatgggcga caagcggttg atttttataa
     3121 agatgtcttt caagcggatt tagtgaatat gatgacatgg gaagagatgg accccaactg
     3181 tcttgaagac cgcaaggatt taatcattaa tgctcagctt atttttgatg gcattcgtct
     3241 tcaaatctcg gatgaaaatc cagatttcgt gtatcaagct ggtaaaaatg ttacagccgc
     3301 tattattgtg ggtagcgtag aggaggcgcg tgaaatttat gaaaaattaa agaagagtgc
     3361 acaagaagtt cagttagagt tacaagaaac tttctggaat cctgcttacg caaacttagt
     3421 agaccaattt ggtgtgatgt ggcaaatttc aacagagtta aactaatcgt ttttaccatc
     3481 tgagcattgc aatgatagat ttataaaaat actgattgta aacagagaag aaaaaattaa
     3541 cggatttcat gtattgaata gctaccaaaa ggtagctatt ttattcctaa ttgaagtaag
     3601 ctattttagc taaagtctat tgtaataggg atttaaaggg gtattagtga taagttgaag
     3661 gcattatgat ataataggtt gaattatatt cagagataga ggaattaata tgtcaaatta
     3721 tgccattatt ttagctgcgg gtaagggaac tcgcatgaaa tctgatttac caaaagttat
     3781 gcataaagta tctggtatta ccatgcttga gcatgttttc cgtagtgtgc aagctattga
     3841 gccaagtaaa attgttactg ttattggaca taaggcggag cttgtacgtg atgttttagg
     3901 tgacaaatca gagtttgtaa tgcaaacaga acagctagga actggccatg ctgtaatgat
     3961 ggcggaagaa gaattagcga catcaaaagg acatacacta gttattgcag gtgatacacc
     4021 cctgattact ggagaaagtt taaaaaactt aattgatttt catgtcaatc ataaaaacgt
     4081 tgcaacaatc ttaacagccg atgctgcaaa tccatttgga tatggtcgta ttatccgtaa
     4141 ttcagatgat gaagttacta aaattgtaga acaaaaggat gctaatgact ttgagcagca
     4201 ggttaaagag attaatactg gaacttatgt ttttgataat caaagtctat ttgaagcact
     4261 taaagacatt aatacaaata acgctcaagg agagtattat ctaactgatg ttattggaat
     4321 tttcaaagaa gcgggtaaaa aggtaggtgc ctataaactt cgtgactttg acgaaagtct
     4381 aggagttaat gatcgtgtag cacttgcaac tgctgaaaaa gtgatgcgtc accgaattgc
     4441 acgtcaacat atggttaatg gggtcacagt ggttaatcct gattccgctt atattgatat
     4501 tgatgttgag attggagaag aatctgtaat tgagcctaat gtgactttaa aaggacagac
     4561 gaagattggt aaaggtactt tattaaccaa tggtagttat ttagtagatg ctcaagtcgg
     4621 aaatgatgtt accatcacta attcgatggt tgaagaatcc attatttctg atggagtaac
     4681 cgtaggccct tatgcgcata ttagaccagg aacaagtctt gcaaaaggtg ttcatattgg
     4741 taatttcgtt gaagtaaagg gttctcaaat aggtgagaat acgaaggcag gtcatttaac
     4801 ttatatcggc aatgcagagg taggttgtga tgttaacttt ggcgcaggga cgattactgt
     4861 caattatgat ggacaaaata aatttaaaac agaaatcgga agtaatgtct ttatcggaag
     4921 taattctact ctgattgcac cacttgaaat tggtgataat gctttaacag cagctggatc
     4981 taccatcaca gataatgttc caattgatag catagctatt ggaagaggac gtcaagtcaa
     5041 taaagaagga tatgccaata aaaaaccaca tcatccttca caaaagtaac acttatttgg
     5101 gaggttctta tggattttga agaaaaaaca attaatcgtc aaactgtttt tgatggacaa
     5161 attatcaaag tagctgttga tgatgtagag ttgccaaatg gattgggaca atccaaacgt
     5221 gaacttgttt ttcatggagg agctgttgct actttggcgg taactcctga gcataaaatt
     5281 gttttagtga aacagtatcg taaggctatt gaaggtattt cttacgaaat tccagcaggg
     5341 aaacttgaga cgggtgagtc aggttctaag gaagaagctg cccttcggga attagaagaa
     5401 gagactggct acacagggaa tcttgaaatt ctttatagtt tctacactgc tattggtttt
     5461 tgtaatgaga aaatcgtcct ctatctagct actgatttac aaaaagttga aaatcctagg
     5521 ccacaagatg atgatgaggt ccttgagctt ctagagttaa gttatgagga ttgtatgcaa
     5581 atggtagaaa aaggcatgat tcaagatgcg aaaacgatca ttgctttaca atattacggt
     5641 ttaaaaatgg gaggtcattc ttaatgggaa aaccattgtt aacagatgat atgattgaac
     5701 gttcaaatcg aggtgaaaaa gtttcaggtc aaacaatctt agatcaagaa actaaaatta
     5761 tttctacaga agatggcatg gagcaactaa ctgacgagaa cggtaaacat atctacaaga
     5821 gtcgccggat tgagaatgca aaacgaaatg aatttcaacg taagttgaat ttagttttat
     5881 tcattttgct tattttgttg gctctcttgt tttatgcaat ttttaaatta taaggaaata
     5941 tcatgaaaat tggaattatt gcagccatgg aggaagagtt aaaactcctt gtggaaaatc
     6001 ttgaggataa atctcaagag acagtattat caaatgtata ttacagtgga cgttatggtg
     6061 aacatgagct tgttttagta cagtctggtg ttgggaaagt catgtcagcg atgagtgttg
     6121 ctattttagt agaaagcttt aaagttgatg ctattattaa tacaggatca gctggtgcag
     6181 tagcaacagg attgaatgta ggagacgtcg ttgtcgcaga tacattagtt tatcacgatg
     6241 tagatttaac cgcctttggt tatgactatg gacaaatgtc aatgcagcca ctttattttc
     6301 attcggataa gacttttgtt tccacttttg aagcagtttt atctaaagaa gaaatgacta
     6361 gtaaagttgg tttaattgca actggggata gttttattgc tggccaggaa aagattgatg
     6421 ttattaaggg gcattttcca caggttttag ctgtcgagat ggaaggtgca gcaattgccc
     6481 aagctgctca agctacagga aagccctttg tggtggtacg tgctatgagt gatactgcag
     6541 ctcatgatgc caatatcaca tttgatgaat ttatcattga agctggtaaa cgctctgccc
     6601 aagtattaat ggcatttttg aaagcattat aaaaaagccc gtgtatctaa tgaatacacg
     6661 gactttttta tttatgtagc agatacatat atttgacttg cgatacgctc agggattgtt
     6721 agattttcat cgttataggt gatgtgataa gtttttgcat aagggtcaat ttgtgtcaat
     6781 tcaatggtat cgttgatatg caaacggtgt tgttctaggt atttcagtag ctgaaaatca
     6841 tcatgaacac gactgataac atatgtgcca agctgactaa tttggtccaa tgtcatttgg
     6901 ttaatttcta ctaaaaactc tcccttttta ggaatagtcc caccgtgtgg acaaaattgt
     6961 ggaaagtcta acaacttttc taattgatca acaaaagttg ttgatacagt atgttctaaa
     7021 acttccgctt cttcatgaat atcatcagca gtatagtgca aatggttaac taaaaatacc
     7081 tctactagac gatgtttacg gtaaaggtca gaaacaacta ataaaccttg tttggtaaga
     7141 taatatccta aatctttatc tttaacaatc agttgttcac ttatcatttt tttaatcatt
     7201 tctgacacgg caggagccga gacttgcatc atttcagcga ttttcttatt ggaaattttg
     7261 ttgttttttt cacctaattc atgaatgcat tttaggtaat cttctttatt tggagtcata
     7321 ttcagtcttt cttatacttc ttaaaatcta tttttctaag accattatat caaaaaatac
     7381 tgaaaaagta tttgacaaat actttataat aggttatgct aatatcaaaa ttaagttaac
     7441 ttaatttaat acataactta tattaatttt aaaaggagca ttttgatgaa aaagtggtta
     7501 gttattgtat catgttttgt agcattttta ggcttaggtg cctgtgctaa caagcaggat
     7561 caagccaaaa acgaaaaatt aaaagtagtt gtaaccaact ctattttggc agatataaca
     7621 aaaaatatag ccggtaacaa aatcgactta catagtattg tacctgttgg taaagatcct
     7681 catgaatatg agccattacc agcagacgtt gaaaaaacaa ctcaagcaga ccttgttttt
     7741 tacaacggaa ttaatcttga aacaggcggt aatgcttggt ttacaaaatt aataaagaac
     7801 gctaagaaga aagaaaacaa agattacttt gcggttagcg atggagttga tgttatttat
     7861 ctaaatggtc aaagcggaaa aggtaaagaa gacccacatg cttggttgaa ccttgaaaat
     7921 ggtattattt actcaaaaaa tattgctaag caattaattg ctaaagatcc aaaaaacaaa
     7981 gctacttatg agaaaaacag agatgcttat gtagctaaat tagaaaaatt ggataaagaa
     8041 gcaaaatcta aatttaatgc cattccagct aataaaaaat taattgttac tagtgaaggt
     8101 tgctttaaat atttctcaaa agcttatgga gttccatcag catacatttg ggaaattaac
     8161 actgaagaag aaggtacacc agatcaaatt actagcttag ttaagaaatt aaaacaagta
     8221 agaccttcag ctttattcgt tgaatcaagt gttgacaagc gtcctatgaa atctgtttca
     8281 agagaatcag gtatcccaat ttacgcagaa atcttcacag actcaattgc taaaaaaggt
     8341 caaaagggtg acagctacta cgctatgatg aaatggaatc ttgataagat tgcagaaggt
     8401 ttagcaaaat aagagtgaga atcgacattg aaatcataat gaaatcaatg atgtagtatt
     8461 ctcgcttaag gctgagttgt aacaatgtcg aaatcctaat ctaaaaactg acttagcttt
     8521 aataaataca gagaagtgga gtctggtttt ataaccagct ccattctact tttggaggaa
     8581 aaatgattat tagtaaacac ttaagcgtaa gttatgataa taatttagtg ctagaagata
     8641 ttaatttacg cttagaaggc tccggaatca ttggtatttt agggcctaat ggggcgggga
     8701 aatctacgct tatgaaagct cttcttggtt tggttgattc tactggagaa tcagggattg
     8761 ggggagattt gctaccttta atggggagag tagcatatgt tgaacaaaaa actaatattg
     8821 attaccaatt ccctataact gttggagaat gcgtttcttt aggattatat aaggaacgag
     8881 gcctcttcaa gcgactatca aaaactgact gggaaaaagt atcccgagtg attgatcaag
     8941 ttggtttgag agggtttgaa aatagaccaa ttaatgcctt atcaggtgga caatttcaac
     9001 gtatgcttat ggcaagatgt ctcgttcaag aagcggatta cattttttta gatgagccgt
     9061 ttgtagggat tgattccatt agtgaacaaa tcatagttaa tttactaaaa aaattaagta
     9121 aggccggaaa attaatttta gtcgtccatc acgatttgag taaagttgat cattattttg
     9181 atcaagtaat tattttgaac cgtcatctaa ttgcttgtgg accaatagat caggcattta
     9241 cccgtgagaa tttaagtgct gcttacggtg atgctatttt gttaggacaa ggagactaga
     9301 atgtttacga aattttttga aggcttattg acttatcatt ttttgcaaaa tgcttttata
     9361 actgcaattg tgattggtat tgtagcgggt gcagtaggct gttttattat cttgcgctca
     9421 atgtctttga tgggtgatgc tatttctcac gctgttctac caggtgttgc tatatctttt
     9481 attttaggga ttaacttttt tataggtgct attgtctttg gtttgttgtc ttcgattatc
     9541 attacttata ttaaagaaaa tagtgtcata aaaggagata ctgctattgg aattactttt
     9601 tcgtcctttt tagccttagg tatcatttta attggacttg ctaatagtac aactgacctc
     9661 tttcatattc tttttgggaa tattttggca gtacaagata gtgataaata tatgactatt
     9721 attgtgggat taattgtact gactctgatt actatcttct ttaaagaatt attattgact
     9781 tcttttgatc ctgttttagc aaaatcaatg ggaatgagag tttcttttta tcattattta
     9841 ttgatgattt tgttaacatt agttgcagta acagctatgc agagtgtggg aacaatattg
     9901 atcgtagcac ttttaattac accagctgca acagcatatc tttatgttaa aagtctcaga
     9961 acaatgttgt ttttatcatc tgcattagga gcagtagcat ctgttctggg gttatatatc
    10021 ggttatacat ttaatattgc cgcaggatca agtattgttt taacatctac ttttatgttt
    10081 ttacttgctt tcctattttc tcctaaacaa agtttgttta aaaagaaaca atagtgttgt
    10141 taaaaaatga gactagaaaa tgttctagtc tcatttttga tagaattatt taaatttgta
    10201 gtctttaata actttgatac ttgtaatagt aattttttct tttggttgat ctgattttgt
    10261 aacttctacc gaagcaattt tatccacagt ttccatgcca gagattactt gtccaaagac
    10321 ggtataacca ccatctaaac ttggatttcc tccctctgaa taggctttaa taatcacctt
    10381 aggaactttt ttatctgaca gttgtttgga atgatcttgc tggctttgat tgataaagaa
    10441 ttggctgcca ttagtatcag ctccagcatt tgccattgct aaacttcccc tgatattgta
    10501 gaggtaaggg gatatttcat taacaaaacc atttccagaa tctttctttt tatctttgct
    10561 attccatata gattttccac cggtcccgtc tccattaggg tcaccagatt gtatcataaa
    10621 atctttgata actctgtgga aacttaaccc attatagtat ccttcttttg catgtgttag
    10681 aaaattttca acagctaaag gagcatattt tggaaataac ttgatattta tatcaccttt
    10741 agaggttttt ataagcactt gtgcctcgtt tttggcaaca gccttgtcca attgaggaaa
    10801 agctttttta tcagctttta aggcttgctt agtttttttg tttagttgtt cagtagcttc
    10861 ctttgaagag ttttcagcta gtttttggtc aacataacgg tctcctttta gagaacgctc
    10921 aattgactcg caaccactta aagttaatat actgacacag gctaaaccaa gataaataat
    10981 ttttttcata catactcctt gattaagtta ttaagatgat tatattttat cataaatttt
    11041 taaataattt ataaaagtaa atagtaaacc tattggggga cttgttgaaa ttatgataga
    11101 atagtatagc atgagttgat agagaaagtg aggaacaagt atttcacttg aaatatggta
    11161 tttatggcaa ataagaaaaa aacaaaagga aagaaaacca gaagacctac taaggcagaa
    11221 atagagcgtc aaagagctat tcaaaggatg attactgctc ttgttttaac aattattctc
    11281 ttctttggta ttatcagatt aggtattttt ggtattacag tctataacgt catccgtttt
    11341 atggtaggta gcttggctta cttatttatt gcggcaactt taatctacct ttatttcttt
    11401 aaatggttgc gaaagaaaga tagcttagta gcaggttttt tgatagcttc tttaggatta
    11461 ttgattgagt ggcatgctta ccttttctca atgcctattt tgaaagataa agaaattttg
    11521 cgttcaactg ctcgattaat tgtgtctgat ttaatgcaat ttaaaatcac tgtttttgcc
    11581 ggtggaggta tgttgggtgc tttgatttac aagccaattg cttttctctt ttctaatatt
    11641 ggtgcctata tgattggtgt tctcttcatc attttgggtc tctttttaat gagttctctg
    11701 gaagtttatg acatcgtcga atttattaga gcttttaaaa ataaagtggc agagaagcac
    11761 gagcaaaata aaaaggagcg ttttgctaag cgagagatga aaaaagcaat cgctgaacaa
    11821 gagcgcatag agcgtcaaaa agctgaagaa gaagcttatt tagcttcggt taatgtagac
    11881 cctgaaacgg gtgagattct agaggatcaa gctgaggaca atttggatga tgcgctacca
    11941 cctgaggtaa gtgaaacatc aactccggta tttgagccag agatccttgc ttatgagaca
    12001 tcgcctcaaa atgatccttt accagtagag ccgacaattt atttagaaga ctatgattcg
    12061 ccgattccta atatgagaga aaatgatgag gaaatggttt atgatttaga tgatgatgta
    12121 gatgatagtg atatagaaaa tgtcgacttt acacctaaaa cgacactggt ttataaatta
    12181 ccaacgatag atttatttgc accagataag cctaaaaatc aatccaaaga aaaggattta
    12241 gtccgaaaga atatcagagt tttagaagaa acatttagaa gttttggtat cgatgtaaaa
    12301 gtagaacgtg ctgaaattgg accatcagtt actaaatatg aaattaaacc agcagttgga
    12361 gttcgtgtga atcgtatttc aaatctatct gacgacctag ctcttgctct tgcagcaaaa
    12421 gatgtgcgta tagaagcacc aattcctgga aaatcattaa taggtattga agttcctaac
    12481 tcagaaattg caacggtttc tttccgcgaa ctttgggaac aatctgatgc caatcctgaa
    12541 aaccttttag aagtaccact aggaaaagct gttaacggca atgctcgcag ttttaactta
    12601 gctagaatgc cgcatctttt ggtagctggt tcaactggtt caggtaaatc tgtggcagtt
    12661 aatggaatta tttcaagtat tttgatgaag gcacgtccag atcaagttaa gtttatgatg
    12721 attgatccca aaatggttga attatctgtt tataatgata ttccacattt attaatccct
    12781 gttgtaacca atccgcgtaa agcaagtaag gcactccaaa aagttgttga tgaaatggaa
    12841 aatcgatacg agttatttag caaaattggt gtgcgtaata tagcaggtta taatacaaag
    12901 gttgaagagt ttaatgcttc ctctgagcaa aaacaaatac ctttgccttt aatcgttgtc
    12961 attgtagatg aattggctga cttgatgatg gttgctagta aagaagttga agatgctatt
    13021 attcgtttgg ggcaaaaagc acgtgctgca ggtatccata tgattcttgc aactcaacgt
    13081 ccatccgtag atgttatttc tggtttgatt aaagcaaatg ttccgtcgcg tattgcattt
    13141 gctgtttcaa gtggtactga tagccgtacg atccttgatg aaaatggtgc tgaaaagctc
    13201 ttgggacggg gtgacatgct ctttaagcct attgatgaga atcatccagt acgactacaa
    13261 ggttccttta tttcagatga tgatgttgaa aggatcgttg gttttatcaa agaccaagcc
    13321 gaggctgact atgatgatgc ctttgatcct ggagaagtat ctgaaacaga taacggctct
    13381 ggtggtggcg gcggagtacc tgaaagtgat cctctttttg aagaagccaa gggactcgtt
    13441 ttagagacgc aaaaagcaag tgcctcaatg attcaacgcc gattgtctgt tggtttcaat
    13501 agagcaacaa gactaatgga agaattagaa gcagcggggg ttattggtcc agcagaagga
    13561 accaagccac gaaaagtttt aatgactcca actccgagtg aataagaaaa aactaatctg
    13621 acaagaaatt gtcggattag ttttttatgt tataatgaag caaatagatg aagaagaagg
    13681 ttacgaatgc atattgagac tgttattgat ttcaaagaat taggaaaaag atatcgtttt
    13741 aaaaatccta caaaagaatt aatagctgat actttagaac aagtcttaga agtgataaaa
    13801 gaagttgatt attatcaatc tcaaaattat tatgttgttg gttatttatc ttatgaagca
    13861 tctgctgctt ttgattcaca ttttaaagtt tctcaacaga agttggctgg agaacatcta
    13921 gcttatttta cagtacataa agattgtgag aacgaagctt ttcctttaag ttatgaaaat
    13981 gttagattag cagataattg gactgctaat gtttctgagc aagaatatca agaggcaatt
    14041 gctaatatta aaggacaaat tagacaagga aatacttatc aagtaaatta tacactagag
    14101 cttagccaac aattatgctc ggatcccttt agtgtttatg aacgcctaat ggtagaacaa
    14161 ggagcaggat ataatgctta tattgcctac gacgataaaa ggattttatc tgtaagcccg
    14221 gaattgttct ttaagaagaa ggatgaagtc ttaacaacta gaccaatgaa gggaacaagc
    14281 gctagaaagc ctacttatca agaagatgtg gcggagcgag attggttggc aaatgatcct
    14341 aaaaatcgtt ctgaaaatat gatgattgtt gatttattaa gaaacgatat gggccgtatc
    14401 tgtgatgttg gaacagttaa ggtaaaaaaa ctatgtcaag tggagcagta tgcaactgtg
    14461 tggcaaatga catcaactat tgaaggagtt ttatcgccag aagtgacact tatgtctatt
    14521 tttcaagctc tatatccttg tggatctatc actggagctc ctaagatatc aacaatggct
    14581 attattaatg agttggaaaa acggccaaga ggtatttatt gtgggacgat aggactttgc
    14641 atgccagacg gacaagctat ttttaacgtt cctattcgca cagtacaaat gaaaggtcaa
    14701 caagcctatt acggcgttgg tggaggtatt acgtgggaga gccagacaga ttctgaatat
    14761 gaagaaaccc gtcaaaaatc agctgtttta acacgtgtca atccaaaatt tcaattaata
    14821 actacaggaa gagtgactga aaataaactt cttttctctc aacaacatgt cgagagatta
    14881 gttgaatcag cgtcttattt tgcttattct tttgataaaa gtaaattcga aagggaactg
    14941 aaaaaatacc ttcatcagct agatgagaag gattaccgct tgaaaataat gcttgataaa
    15001 actggtaagg taacgtttga ggtaaaacaa ctagtgaatt tatcaaaaaa gtttttaacg
    15061 gcagaggtgg tcgtacaaga ctaccctatt aaattatccc cgtttactta ttttaaaact
    15121 tcttatcgcc cacatattat tgaaggtcag aatgaaaaga tatttgtatc tcctgagggg
    15181 ttgctattgg aaacaagtat tgggaatatt gttttagaaa aaaatggaag gtttttaacc
    15241 ccagatttat cagaaggagg gttgaatggg atttatcgtc gtcatctcct taaaaaccaa
    15301 aaagtaattg aagcaccact aactttaaaa gatttagaat cagccgatgc tatatacgcc
    15361 tgtaatgctg ttagagggct ttatcctcta aacctaaagt aaaaagagtt gga
//
LOCUS       DATROD010000036        14603 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.036, whole genome shotgun sequence.
ACCESSION   DATROD010000036 DATROD010000000
VERSION     DATROD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14603
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.036"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            221..553
                     /locus_tag="VBC74_001060"
     CDS             221..553
                     /locus_tag="VBC74_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEO6942020.1"
                     /translation="MLRKYKQRNYNVAHTKQRFSIKKFKFGVASVLIGLTFLGMSSHS
                     VLADESSIMSTEVPQTAGVKPEVTVTQPTSDVTSLATDTSTSAVLSTDASHSNPTESA
                     EHAVGLEC"
     gene            complement(674..1818)
                     /locus_tag="VBC74_001061"
     CDS             complement(join(674..1584,1584..1818))
                     /locus_tag="VBC74_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSag5 family transposase"
                     /protein_id="HEO6942021.1"
                     /translation="MSTFKRYDEEFKQSLVNLYQTGKTQSELCKDYRVSTSALAKWIK
                     QYSQFKLEDNSVLTAKQIQELQKRNAQLEEENLIPKKSKCHIHAKLKVRLLAVYRLRF
                     EHATTTLCRVLRVNRSTYYKFLKHKPSKRELDNQIYKKQILEIYTKANKRLGVKSIKV
                     ILQRDYDTKISEGRIYHLMKNMALPKMATVKPKTALKKTQKTYPQNLLNQKFYPDKPN
                     QVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHRMDAKLACNTLELALNKRKI
                     EGTLLFHSDQGSQFKAREFRKIIDDNNIMHSFSKPGYPYDNAVTEAFFKYLKHRQINR
                     KHYQNIKQVQLDCFEYIENFYNNYIPHTANLGLTPNQKEENYFNAIK"
     gene            1951..2088
                     /locus_tag="VBC74_001062"
     CDS             1951..2088
                     /locus_tag="VBC74_001062"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942022.1"
                     /translation="MIIARENHQVTHEHVSILLTCVQELIVEVNQTQPLSREFREKYM
                     G"
     gene            2098..2353
                     /gene="mobC"
                     /locus_tag="VBC74_001063"
                     /pseudo
     CDS             2098..2353
                     /gene="mobC"
                     /locus_tag="VBC74_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
     gene            complement(2328..2823)
                     /locus_tag="VBC74_001064"
                     /pseudo
     CDS             complement(2328..2823)
                     /locus_tag="VBC74_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
     gene            complement(3017..4225)
                     /locus_tag="VBC74_001065"
     CDS             complement(3017..4225)
                     /locus_tag="VBC74_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO6942023.1"
                     /translation="MVTFDFKHVQTLHTISQLPISVMSRDKELVQLYGNEDYLLPYYQ
                     FLKHLAIPYNQDITVYEGLFEESFLIFPVCQYLIAIGPFYPYSPDINRQEQLSSRFLE
                     QFSHRSKKEILSYIKLVPCFPTTSIRSLLVSIDAFFQTQFEASCQQVINHLLEESEQI
                     VADPDIVLHLKHTKKNSFQLPTVLNHLNHIIDLVKLGNTQLLKQEINRLPSSSVTSSS
                     IPALRAEKNLTVVYLTKLLELSFEENTDVAKSYALVKHYMALNEEAPDLIDVSRIRCA
                     ALIDFSESLTNKSISDKQQMYNSILHYVDNHLYSKLKVSDIANYLYISDSHLRSVFKK
                     YSDISLQSYILKAKIKEGQLLLQRGVPIGEVAKLLHFYDTTHFLKTFKKYVGISSNEY
                     LTKYRETSCQ"
     gene            4606..6270
                     /locus_tag="VBC74_001066"
     CDS             4606..6270
                     /locus_tag="VBC74_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEO6942024.1"
                     /translation="MKLSKKLLFSAAVLTMVAGSTVEPVAQFATGMSIVRAAEVSQER
                     PAKTTVNIYKLQADSYKSEITSNGGIENKDGEVISNYAKLGDNVKGLQGVQFKRYKVK
                     TDISVDELKKLTTVEAADAKVGTILEEGVSLPQKTNAQGLVVDALDSKSNVRYLYVED
                     LKNSPSNITKAYAVPFVLELPVANSTGTGFLSEINIYPKNVVTDEPKTDKDVKKLGQD
                     DAGYTIGEEFKWFLKSTIPANLGDYEKFEITDKFADGLTYKSVGKIKIGSKTLNRDEH
                     YTIDEPTVDNQNTLKITFKPEKFKEIAELLKGMTLVKNQDALDKATANTDDAAFLEIP
                     VASTINEKAVLGKAIENTFELQYDHTPDKADNPKPSNPPRKPEVHTGGKRFVKKDSTE
                     TQTLGGAEFDLLASDGTAVKWTDALIKANTNKNYIAGEAVTGQPIKLKSHTDGTFEIK
                     GLAYAVDANAEGTAVTYKLKETKAPEGYVIPDKEIEFTVSQTSYNTKPTDITVDSADA
                     TPDTIKNNKRPSIPNTGGIGTAIFVAIGAAVMAFAVKGMKRRTKDN"
     gene            6359..7282
                     /locus_tag="VBC74_001067"
     CDS             6359..7282
                     /locus_tag="VBC74_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6942025.1"
                     /translation="MKQTLKLMFSFLLMLGTMFGISQTVLAQETHQLTIVHLEARDID
                     RPNPQLEIAPKEGTPIEGVLYQLYQLKSTEDGDLLAHWNSLTITELKKQAQQVFEATT
                     NQQGKATFNQLPDGIYYGLAVKAGEKNRSVSAFLVDLSEDKVIYPKIIWSTGELDLLK
                     VGVDGDTKKPLAGVVFELYEKNGRTPIRVKNGVHSQDIDAAKHLETDSSGHIRISGLI
                     HGDYVLKEIETQSGYQIGQAETAVTIEKSKTVTVTIENKKVPTPKVPSRGGLIPKTGE
                     QQAMALVIIGGILIALALRLLSKHRKHQNKD"
     gene            7284..8201
                     /gene="srtC1"
                     /locus_tag="VBC74_001068"
     CDS             7284..8201
                     /gene="srtC1"
                     /locus_tag="VBC74_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC1"
                     /protein_id="HEO6942026.1"
                     /translation="MGQKSKISLATNIRIWIFRLIFLAGFLVLAFPIVSQVMYFQASH
                     ANINAFKEAVTKIDRVEINRRLELAYAYNASIAGAKTNGEYPALKDPYSAEQKQAGVV
                     EYARMLEVKEQIGHVIIPRINQDIPIYAGSAEENLQRGVGHLEGTSLPVGGESTHAVL
                     TAHRGLPTAKLFTNLDKVTVGDRFYIEHIGGKIAYQVDQIKVIAPDQLEDLYVIQGED
                     HVTLLTCTPYMINSHRLLVRGKRIPYVEKTVQKDSKTFRQQQYLTYAMWVVVGLILLS
                     LLIWFKKTKQKKRRKNEKAASQNSHNNSK"
     gene            8158..9009
                     /gene="srtC2"
                     /locus_tag="VBC74_001069"
     CDS             8158..9009
                     /gene="srtC2"
                     /locus_tag="VBC74_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase SrtC2"
                     /protein_id="HEO6942027.1"
                     /translation="MKKRLVKIVTIIRNNKIRTLIFVMGSLILLFPIVSQVSYYLASH
                     QNINQFKREVAKIDTNTVERRIALANAYNETLSRNPLLIDPFTSKQKEGLREYARMLE
                     VHEQIGHVAIPSIGVDIPIYAGTSETVLQKGSGHLEGTSLPVGGLSTHSVLTAHRGLP
                     TARLFTDLNKVKKGQIFYVTNIKETLAYKVVSIKVVDPTALSEVKIVNGKDYITLLTC
                     TPYMINSHRLLVKGERIPYDSTEAEKHKEQTVQDYRLSLVLKILLVLLIGLFIVIMMR
                     RWMQHRQ"
     gene            9091..11763
                     /locus_tag="VBC74_001070"
     CDS             9091..11763
                     /locus_tag="VBC74_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEO6942028.1"
                     /translation="MKKRQKIWRGLSVTLLILSQIPFGILVQGETQDTNQALGKVIVK
                     KTGDNATPLGKATFVLKNDNDKSETSHETVEGSGEATFENIKPGDYTLREETAPIGYK
                     KTDKTWKVKVADNGATIIEGMDADKAEKRKEVLNAQYPKSAIYEDTKENYPLVNVEGS
                     KVGEQYKALNPINGKDGRREIAEGWLSKKITGVNDLDKNKYKIELTVEGKTTVETKEL
                     NQPLDVVVLLDNSNSMNNERANNSQRALKAGEAVEKLIDKITSNKDNRVALVTYASTI
                     FDGTEATVSKGVADQNGKALNDSVSWDYHKTTFTATTHNYSYLNLTNDANEVNILKSR
                     IPKEAEHINGDRTLYQFGATFTQKALMKANEILETQSSNARKKLIFHVTDGVPTMSYA
                     INFNPYISTSYQNQFNSFLNKIPDRSGILQEDFIINGDDYQIVKGDGESFKLFSDRKV
                     PVTGGTTQAAYRVPQNQLSVMSNEGYAINSGYIYLYWRDYNWVYPFDPKTKKVSATKQ
                     IKTHGEPTTLYFNGNIRPKGYDIFTVGIGVNGDPGATPLEAEKFMQSISSKTENYTNV
                     DDTNKIYDELNKYFKTIVEEKHSIVDGNVTDPMGEMIEFQLKNGQSFTHDDYVLVGND
                     GSQLKNGVALGGPNSDGGILKDVTVTYDKTSQTIKINHLNLGSGQKVVLTYDVRLKDN
                     YISNKFYNTNNRTTLSPKSEKEPNTIRDFPIPKIRDVREFPVLTISNQKKMGEVEFIK
                     VNKDKHSESLLGAKFQLQIEKDFSGYKQFVPEGSDVTTKNDGKIYFKALQDGNYKLYE
                     ISSPDGYIEVKTKPVVTFTIQNGEVTNLKADPNANKNQIGYLEGNGKHLITNTPKRPP
                     GVFPKTGGIGTIVYILVGSTFMILTICSFRRKQL"
     gene            11817..12607
                     /locus_tag="VBC74_001071"
                     /pseudo
     CDS             11817..12607
                     /locus_tag="VBC74_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
     gene            12856..13461
                     /locus_tag="VBC74_001072"
     CDS             12856..13461
                     /locus_tag="VBC74_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HEO6942029.1"
                     /translation="MARDPYSDRSRYLDPKVLSSSFGAFFPADNIKVAWSNNSSTLFT
                     PPINANYTTQIQAIGTTIKSQIPESILTVTDKKSRKFSINKIDEAKEGLVGATFTLSK
                     RTTVAADHQVQGDFIPVSKETTVGRTTLTFDNLKPGFYDLKETKAPNAYVLDPKTYVV
                     VVQNSGKTTIVDEANFKEADYPMADNTSHVECVALLQRSKG"
     gene            13982..14172
                     /locus_tag="VBC74_001073"
                     /pseudo
     CDS             13982..14172
                     /locus_tag="VBC74_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883557.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
BASE COUNT         4939 a         2362 c         2942 g         4360 t
ORIGIN      
        1 tttaaaaatt ttaaattagt gttatctgaa cttaccaata tactatcacc tttatataaa
       61 atcttaaagt tataaatctt atcgaatgtg attatacaat acaaattcaa ataatagttc
      121 acatttatcg agataatgta aaacagtaaa actttaaaaa aagtaaaaat agataacttt
      181 attggttaat ctatttttac ttttgaaaag gagagaagtt atgcttagga agtataagca
      241 aagaaattat aatgtcgcac atacgaaaca gcgtttttcg attaagaaat tcaaatttgg
      301 tgtagcatcg gtattaattg gattgacatt tctaggaatg agtagtcatt ctgttttggc
      361 ggatgagtca agcattatga gtactgaggt accacaaaca gctggtgtaa aaccggaagt
      421 tactgttaca cagcctactt cagatgtaac aagtcttgcg acagatacgt caacttcagc
      481 agtgttatca acagatgcat ctcattctaa tcctacagaa tcagcggagc atgctgtagg
      541 attagaatgc tgaagatgca gcggttccag tgacacacga aaaagaagca aataaaacat
      601 tgcgagttac ttcttcttcc acatctgaat tcacagagtg gactaatgtc aaataagtag
      661 acagaaaacc gtgttatttt attgcgttaa aataattttc ttctttctga ttaggggtta
      721 gtcctagatt agccgtatgt gggatgtaat tgttataaaa attctcaatg tattcaaagc
      781 agtctaattg aacctgtttg atattttgat aatgttttcg gttgatttgt ctatgcttta
      841 aatacttgaa aaatgcttca gttacggcat tatcataagg atatccaggt ttagaaaaag
      901 aatgcatgat attgttgtca tcaattattt ttctaaattc cctggcctta aattgtgacc
      961 cttggtcgga atgaaagaga agtgttcctt caatctttct tttattaaga gctaattcta
     1021 gagtgttaca tgctaacttt gcatccatac ggtgactcaa tttccaagca atacattttc
     1081 tagaatagag atcaagtatt gcgcagagat agacatattt cttatatcca atagaaatat
     1141 aggtgaagtc agtagaccat acttgattag gtttgtcagg ataaaatttc tggttgagta
     1201 agttttgagg atacgttttt tgagtctttt taagagctgt ttttggctta acggtagcca
     1261 ttttagggag cgccatattc ttcatcagat ggtaaattct tccttcagag atttttgtgt
     1321 cgtagtctct ttgaagaatg actttgatag acttcacacc aagtcttttg tttgctttgg
     1381 tataaatctc aagtatttgt tttttataaa tttgattatc caattctctt tttgagggct
     1441 tatgttttag aaatttatag taagtggagc gattgacacg taaaacacga cacaaggttg
     1501 ttgtggcgtg ttcaaagcgt aaccgataga cagcgaggag tcttacttta agttttgcat
     1561 gaatatggca cttgcttttt ttaggataag gttctcctct tcaagctgcg cattacgttt
     1621 ttgtaactct tgtatttgct tggcagtaag tacagaatta tcttcgagtt tgaattgaga
     1681 gtactgtttg atccattttg caagcgcaga agtggatacc ctatagtctt tacagagttc
     1741 agactgagtt tttccagttt gataaaggtt aacaagagat tgtttgaatt cttcatcata
     1801 gcgtttaaat gttgacatag gttgtccttt cgtttttgtg tcttttaaaa cagattgtaa
     1861 cacacaaagt tttgtctact tttatagtat acatccaccc tttggcaata ccaaaagttt
     1921 gagcagatta gttgagatgt gcaggaagtt ttgattatcg caagagagaa tcatcaagtg
     1981 actcatgaac acgtgtcaat cttattgacc tgcgttcaag aattgattgt ggaagtcaat
     2041 caaacacagc cactcagtcg tgagtttcgt gaaaagtata tgggttagga ggctgcaatt
     2101 gtttatcgtt atcgtaccaa tctcaaaaaa gtctttctaa cagattcaga attgaatcaa
     2161 ttaaatgagc ggattgctaa gagcaattgt caaaatttct cagtctatgc tagaagagtt
     2221 cttcttaatc ccaatatgtc atttgtcacg attaacacgg attggaaata acatcaatca
     2281 aattgcgcgt gctatcaacc aaagccatct aatttctcag gctcagtatc actttcatcg
     2341 agaagcacgt taagcccaac agcagggggg gttgctctga ttcggttaag taataagcaa
     2401 aatcctcacc aattttccca gtaataagag gcgttgttga ggtataggga ttacctgtgc
     2461 catagtcgat gatggtcaca aaatgaccat tccccataaa agggccaacc aaaacttcac
     2521 ctgtggccat tttttgatgt caacaccagt attttggatg taccccttaa catgcccttt
     2581 ggtatctgca acagagataa tatgaccaaa ggaagaatca ccgatgactt taaccgtaat
     2641 cttgctatcg cctttttgat tagccgctaa tatttgatta gcaatcaggg tgcgaccaag
     2701 agcaactgtt gaagacgaca aggtatgatg tttttcctga gccaatttaa cagtctcggt
     2761 gctatcgagg acataagacc tgaaggcacc agaggcagaa attgatttaa taattttatc
     2821 cataacctat gttatagcac aaagagagag tttgtatgga ctggattagc tgaaaatttt
     2881 agactaaaaa gtagtgccag tggattttga aaggtaacaa aagagtcttc cgtttaaacg
     2941 agaaggctgt ttttagtcgt ttcagatgaa ggcaaaagga cgatgaaata attccgtacc
     3001 ttcatggatt gctatgttac tggcatgagg tctcacgata tttagtaaga tattcgtttg
     3061 aagatattcc cacgtatttt ttaaaggttt taagaaaatg tgtggtgtcg taaaaatgta
     3121 ataatttcgc tacttcccca atcggtaccc ctctttgcag tagtaattgt ccctccttaa
     3181 tttttgcctt tagaatataa ctttgcaagg aaatgtcaga gtatttttta aaaactgagc
     3241 gtaagtggga atctgagata tataggtagt tggcaatatc agatactttg agtttggagt
     3301 agaggtggtt gtccacataa tggagaatac tattgtacat ttgctgcttg tcagagatgc
     3361 tcttattggt taaggattct gaaaaatcaa taagagctgc acagcgaatt cttgaaacat
     3421 caataagatc aggagcctct tcgtttaaag ccatatagtg ctttaccagc gcataacttt
     3481 tagccacatc agtattttcc tcgaaactta attctagtaa ttttgttaag taaacaacag
     3541 ttaagttctt ttcagctctt agggcaggga ttgaagatga ggtaacactg gatgatggga
     3601 ggcgattaat ttcttgcttt aacagttgag tgttacccag cttaacgaga tcaataatgt
     3661 gattgagatg gtttaaaaca gtgggtaact gaaaagagtt tttcttagta tgttttaggt
     3721 gaagaacaat atcaggatcc gcaacaatct gttctgactc ttctaataaa tgattgatga
     3781 cttgttggca actagcctca aactgtgttt ggaaaaaggc atcgatagac acaagaagac
     3841 tacgtatact ggtagtagga aaacaaggga caagctttat ataggataag atttcttttt
     3901 tactacgatg agaaaattgt tctagaaagc gactggataa ctgttcttgc ctattgatat
     3961 cagggctata gggataaaat ggtccaatag caataagata ttgacagaca ggaaaaatta
     4021 agaatgattc ttcaaaaaga ccctcataaa cagtgatatc ttggttataa gggatagcta
     4081 aatgttttaa aaactgatag taaggcaaca gatagtcttc gttaccatat aactgaacga
     4141 gttccttgtc tcgtgacatg actgaaatag gtagttgaga tatggtatgc aatgtttgaa
     4201 catgtttaaa atcgaatgta accatttgat agaccgcctt cattatcatt tctagaattt
     4261 ttctttaggt ttgtaaagac tacaaaataa aatgatgaaa acaactatct tgtggataca
     4321 ctaaaaagac acgctaatta gcaaactctc tcttcatcat ctctcaccat tattatacta
     4381 ctatttatat gacaaataaa ggtgattttg ttaaaatata actttgaaaa tccacatata
     4441 tttttaatct tccgtctgaa aaaataaata aaaatagtaa aaataaacac gaatttaaaa
     4501 taagcaaatt ttttaagaaa atctgtgcta aactttaata gttttgtgct taataataat
     4561 cagcacttac aaagaacaaa gggaaaagcg aggagagaac ttttaatgaa attatcgaag
     4621 aagttattgt tttcggctgc tgttttaaca atggtggcgg ggtcaactgt tgaaccagta
     4681 gctcagtttg cgactggaat gagtattgta agagctgcag aagtgtcaca agaacgccca
     4741 gcgaaaacaa cagtaaatat ctataaatta caagctgata gttataaatc ggaaattact
     4801 tctaatggtg gtatcgagaa taaagacggc gaagtaatat ctaactatgc taaacttggt
     4861 gacaatgtaa aaggtttgca aggtgtacag tttaaacgtt ataaagtcaa gacggatatt
     4921 tctgttgatg aattgaaaaa attgacaaca gttgaagcag cagatgcaaa agttggaacg
     4981 attcttgaag aaggtgtcag tctacctcaa aaaactaatg ctcaaggttt ggtcgtcgat
     5041 gctctggatt caaaaagtaa tgtgagatac ttgtatgtag aagatttaaa gaattcacct
     5101 tcaaacatta ccaaagctta tgctgtaccg tttgtgttgg aattaccagt tgctaactct
     5161 acaggtacag gtttcctttc tgaaattaat atttacccta aaaacgttgt aactgatgaa
     5221 ccaaaaacag ataaagatgt taaaaaatta ggtcaggacg atgcaggtta tacgattggt
     5281 gaagaattca aatggttctt gaaatctaca atccctgcca atttaggtga ctatgaaaaa
     5341 tttgaaatta ctgataaatt tgcagatggc ttgacttata aatctgttgg aaaaatcaag
     5401 attggttcga aaacactgaa tagagatgag cactacacta ttgatgaacc aacagttgat
     5461 aaccaaaata cattaaaaat tacgtttaaa ccagagaaat ttaaagaaat tgctgagcta
     5521 cttaaaggaa tgacccttgt taaaaatcaa gatgctcttg ataaagctac tgcaaataca
     5581 gatgatgcgg catttttgga aattccagtt gcatcaacta ttaatgaaaa agcagtttta
     5641 ggaaaagcaa ttgaaaatac ttttgaactt caatatgacc atactcctga taaagctgac
     5701 aatccaaaac catctaatcc tccaagaaaa ccagaagttc atactggtgg gaaacgattt
     5761 gtaaagaaag actcaacaga aacacaaaca ctaggtggtg ctgagtttga tttgttggct
     5821 tctgatggga cagcagtaaa atggacagat gctcttatta aagcgaatac taataaaaac
     5881 tatattgctg gagaagctgt tactgggcaa ccaatcaaat tgaaatcaca tacagacggt
     5941 acgtttgaga ttaaaggttt ggcttatgca gttgatgcga atgcagaggg tacagcagta
     6001 acttacaaat taaaagaaac aaaagcacca gaaggttatg taatccctga taaagaaatc
     6061 gagtttacag tatcacaaac atcttataat acaaaaccaa ctgacatcac ggttgatagt
     6121 gctgatgcaa cacctgatac aattaaaaac aacaaacgtc cttcaatccc taatactggt
     6181 ggtattggta cggctatctt tgtcgctatc ggtgctgcgg tgatggcttt tgctgttaag
     6241 gggatgaagc gtcgtacaaa agataactaa ataaaaggct acttcttaag taaccatgtt
     6301 taagaaaaga gaaatagcct tatttctctt tttgtcgttt ttaaaataaa ggaacatcat
     6361 gaaacaaaca ttaaaactta tgttttcttt tctgttgatg ttagggacta tgtttggaat
     6421 tagccaaact gttttagcgc aagaaactca tcagttgacg attgttcatc ttgaagcaag
     6481 ggatattgat cgtccaaatc cacagttgga gattgcccct aaagaaggga ctccaattga
     6541 aggagtactc tatcagttgt accaattaaa atcaactgaa gatggcgatt tgttggcaca
     6601 ttggaattcc ctaactatca cagaattgaa aaaacaggcg cagcaggttt ttgaagccac
     6661 tactaatcaa caaggaaagg ctacatttaa ccaactacca gatggaattt attatggtct
     6721 ggcggttaaa gccggtgaaa aaaatcgtag tgtctcagct ttcttggttg acttgtctga
     6781 ggataaagtg atttatccta aaatcatctg gtccacaggt gagttggact tgcttaaagt
     6841 tggtgtggat ggtgatacca aaaaaccact agcaggcgtt gtctttgaac tttatgaaaa
     6901 gaatggtagg actcctattc gtgtgaaaaa tggggtgcat tctcaagata ttgacgctgc
     6961 aaaacattta gaaacagatt catcagggca tatcagaatt tccgggctca tccatgggga
     7021 ctatgtctta aaagaaatcg agacacagtc aggatatcag atcggacagg cagagactgc
     7081 tgtgactatt gaaaaatcaa aaacagtaac agtaacgatt gaaaataaaa aagttccgac
     7141 acctaaagtg ccatctcgag gaggtcttat tcccaaaaca ggtgagcaac aggcaatggc
     7201 acttgtaatt attggtggta ttttaattgc tttagcctta cgattactat caaaacatcg
     7261 gaaacatcaa aataaggatt agcatgggac aaaaatcaaa aatatctcta gctacgaata
     7321 ttcgtatatg gatttttcgt ttaattttct tagcgggttt ccttgttttg gcatttccca
     7381 tcgttagtca ggtcatgtac tttcaagcct ctcacgccaa tattaatgct tttaaagaag
     7441 ctgttaccaa gattgaccgg gtggagatta atcggcgttt agaacttgct tatgcttata
     7501 acgccagtat agcaggtgcc aaaactaatg gcgaatatcc agcgcttaaa gacccctact
     7561 ctgctgaaca aaagcaggca ggggtcgttg agtacgcccg catgcttgaa gtcaaagaac
     7621 aaataggtca tgtgattatt ccaagaatta atcaggatat ccctatttac gctggctctg
     7681 ctgaagaaaa tcttcagagg ggcgttggac atttagaggg gaccagtctt ccagtcggtg
     7741 gtgagtcaac tcatgccgtt ctaactgccc atcgagggct accaacggcc aagctattta
     7801 ccaatttaga caaggtaaca gtaggtgacc gtttttacat tgaacacatc ggcggaaaga
     7861 ttgcttatca ggtagaccaa atcaaagtta tcgcccctga tcagttagag gatttgtacg
     7921 tgattcaagg agaagatcac gtcaccctat taacttgcac accttatatg ataaatagtc
     7981 atcgcctcct cgttcgaggc aagcgaattc cttatgtgga aaaaacagtg cagaaagatt
     8041 caaagacctt caggcaacaa caatacctaa cctatgctat gtgggtagtc gttggactta
     8101 tcttgctgtc gcttctcatt tggtttaaaa agacgaaaca gaaaaagcgg agaaagaatg
     8161 aaaaagcggc tagtcaaaat agtcacaata attcgaaata ataaaattag aaccctcatt
     8221 tttgtgatgg gaagtctgat tctcttattt ccgattgtga gccaggtaag ttactacctt
     8281 gcttcgcatc aaaatattaa tcaatttaag cgggaagtcg ctaagattga tactaatacg
     8341 gttgaacgac gcatcgcttt agctaatgct tacaatgaga cgttatcaag gaatcccttg
     8401 cttatagacc cttttaccag taagcaaaaa gaaggtttga gagagtatgc tcgtatgctt
     8461 gaagttcatg agcaaatagg tcatgtggca atcccaagta ttggggttga tattccaatt
     8521 tatgctggaa catccgaaac tgtgcttcag aaaggtagtg ggcatttgga gggaaccagt
     8581 cttccagtgg gaggtttgtc aacccattca gtactaactg cccaccgtgg cttgccaaca
     8641 gctaggctat ttaccgactt aaataaagtt aaaaaaggcc agattttcta tgtgacgaac
     8701 atcaaggaaa cacttgccta caaagtcgtg tctatcaaag ttgtggatcc aacagcttta
     8761 agtgaggtta agattgtcaa tggtaaggat tatataacct tgctgacttg cacaccttac
     8821 atgatcaata gtcatcgtct cttggtaaaa ggagagcgta ttccttatga ttctaccgag
     8881 gcggaaaagc acaaagaaca aaccgtacaa gattatcgtt tgtcactagt gttgaagata
     8941 ctactagtat tattaattgg actcttcatc gtgataatga tgagaagatg gatgcaacat
     9001 cgtcaataac gatgttgtga atggcttact tacttatcaa ataggtgact aatgatgatt
     9061 gtgaataatg gttatctaga agggagaaaa atgaaaaaga gacaaaaaat atggagaggg
     9121 ttatcagtta ctttactaat cctgtcccaa attccatttg gtatattggt acaaggtgaa
     9181 acccaagata ccaatcaagc acttggaaaa gtaattgtta aaaaaacggg agacaatgct
     9241 acaccattag gcaaagcgac ttttgtgtta aaaaatgaca atgataagtc agaaacaagt
     9301 cacgaaacgg tagagggttc tggagaagca acctttgaaa acataaaacc tggagactac
     9361 acattaagag aagaaacagc accaattggt tataaaaaaa ctgataaaac ctggaaagtt
     9421 aaagttgcag ataacggagc aacaataatc gagggtatgg atgcagataa agcagagaaa
     9481 cgaaaagaag ttttgaatgc ccaatatcca aaatcagcta tttatgagga tacaaaagaa
     9541 aattacccat tagttaatgt agagggttcc aaagttggtg aacaatacaa agcattgaat
     9601 ccaataaatg gaaaagatgg tcgaagagag attgctgaag gttggttatc aaaaaaaatt
     9661 acaggggtca atgatctcga taagaataaa tataaaattg aattaactgt tgagggtaaa
     9721 accactgttg aaacgaaaga acttaatcaa ccactagatg tcgttgtgct attagataat
     9781 tcaaatagta tgaataatga aagagccaat aattctcaaa gagcattaaa agctggggaa
     9841 gcagttgaaa agctgattga taaaattaca tcaaataaag acaatagagt agctcttgtg
     9901 acatatgcct caaccatttt tgatggtact gaagcgaccg tatcaaaggg agttgccgat
     9961 caaaatggta aagcgctgaa tgatagtgta tcatgggatt atcataaaac tacttttaca
    10021 gcaactacac ataattacag ttatttaaat ttaacaaatg atgctaacga agttaatatt
    10081 ctaaagtcaa gaattccaaa ggaagcggag catataaatg gggatcgcac gctctatcaa
    10141 tttggtgcga catttactca aaaagctcta atgaaagcaa atgaaatttt agagacacaa
    10201 agttctaatg ctagaaaaaa acttattttt cacgtaactg atggtgtccc tacgatgtct
    10261 tatgccataa attttaatcc ttatatatca acatcttacc aaaaccagtt taattctttt
    10321 ttaaataaaa taccagatag aagtggtatt ctccaagagg attttataat caatggtgat
    10381 gattatcaaa tagtaaaagg agatggagag agttttaaac tgttttcgga tagaaaagtt
    10441 cctgttactg gaggaacgac acaagcagct tatcgagtac cgcaaaatca actctctgta
    10501 atgagtaatg agggatatgc aattaatagt ggatatattt atctctattg gagagattac
    10561 aactgggtct atccatttga tcctaagaca aagaaagttt ctgcaacgaa acaaatcaaa
    10621 actcatggtg agccaacaac attatacttt aatggaaata taagacctaa aggttatgac
    10681 atttttactg ttgggattgg tgtaaacgga gatcctggtg caactcctct tgaagctgag
    10741 aaatttatgc aatcaatatc aagtaaaaca gaaaattata ctaatgttga tgatacaaat
    10801 aaaatttatg atgagctaaa taaatacttt aaaacaattg ttgaggaaaa acattctatt
    10861 gttgatggaa atgtgactga tcctatggga gagatgattg aattccaatt aaaaaatggt
    10921 caaagtttta cacatgatga ttacgttttg gttggaaatg atggcagtca attaaaaaat
    10981 ggtgtggctc ttggtggacc aaacagtgat gggggaattt taaaagatgt tacagtgact
    11041 tatgataaga catctcaaac catcaaaatc aatcatttga acttaggaag tggacaaaaa
    11101 gtagttctta cctatgatgt acgtttaaaa gataactata taagtaacaa attttacaat
    11161 acaaataatc gtacaacgct aagtccgaag agtgaaaaag aaccaaatac tattcgtgat
    11221 ttcccaattc ccaaaattcg tgatgttcgt gagtttccgg tactaaccat cagtaatcag
    11281 aagaaaatgg gtgaggttga atttattaaa gttaataaag acaaacattc agaatcgctt
    11341 ttgggagcta agtttcaact tcagatagaa aaagattttt ctgggtataa gcaatttgtt
    11401 ccagagggaa gtgatgttac aacaaagaat gatggtaaaa tttattttaa agcacttcaa
    11461 gatggtaact ataaattata tgaaatttca agtccagatg gctatataga ggttaaaacg
    11521 aaacctgttg tgacatttac aattcaaaat ggagaagtta cgaacctgaa agcagatcca
    11581 aatgctaata aaaatcaaat cgggtatctt gaaggaaatg gtaaacatct tattaccaac
    11641 actcccaaac gcccaccagg tgtttttcct aaaacagggg gaattggtac aattgtctat
    11701 atattagttg gttctacttt tatgatactt accatttgtt ctttccgtcg taaacaattg
    11761 taaggtgtcg ttgaaattat taaataatag aaaatgacta gttttggcct ttccctattg
    11821 tcagtcagat tagttattat caagcttctc atgccaatat taatgccttt aaaagaacag
    11881 ttacaaccat tgaccgtacg gagattcaac gacggttagg tctggctaaa gcctacaatg
    11941 ctagtatttc tggaacaagt agtcagtcga ctcaatctgt gctgagagat tcttattctg
    12001 aggagcaaaa aaggcagggt ggactgaata cgctaggatg ttagaagtca gagagcaggt
    12061 tgaccatgtg atgattccaa aaatcaatca ggatttacca atctacgctg gttcagaaga
    12121 ggacaatctg caacggggag ttggtcatct agaagggata agtttgccga ttggaggggc
    12181 ttctacacat gcggtcttga gcggtcaaag aggtatgcca gctgctcggt tgtttgcgga
    12241 tttggataag atgaaaaaag gtgattattt ttatgttacc aatctgaaag aaaccttggc
    12301 ttatcaagtg gatcgtatca tggtgattga acctagccaa ttggatgccg tgagcattga
    12361 agaggataaa gattatgtta cccttctgac ctgtacacct tatatgggct ctttgtcaac
    12421 tgtaatgggt gacttatcat taacaacgag agagaatcag cttggttctc tctctttttg
    12481 gatgttcaaa gcgatgagaa ttttgctttt aaaattttta aagttgcgaa agccaaaggc
    12541 ttgtcgcttg atgtctttga taagcttgtt ggtcgcttct aatttggcat tggaaaggga
    12601 tagctgaggg cgtttgtgat gtaagttttg tacttcatga aggtcctaaa gaccatttta
    12661 aaagcagaat tgccagttgt tacgttatca tctatgaagc taaagaattc agttgttcgc
    12721 ttttcttaga aatgaaagag tatgagctga taaaggtcgt tataatagcg gagctcatct
    12781 gagaaaactt ttatacctca aagtcagtct agctttgata tcatgaagcc attaggagtt
    12841 attccttatc ttttagtggc gcgcgatcca tatagtgata gatcgagata tttagatcca
    12901 aaagttctat catcctcttt tggcgccttt tttccagcag ataatattaa ggtagcttgg
    12961 tctaacaact ccagcacttt atttacacca cctattaatg caaactacac cactcagatt
    13021 caagctattg ggacaacgat taagtcacaa attccggaat cgattttgac ggttacggat
    13081 aaaaagagca ggaagttcag cattaacaag attgacgaag ctaaagaagg cttagtaggt
    13141 gcgaccttca ccttgtctaa acgcacaaca gtagcggcag atcatcaagt acaaggagat
    13201 ttcattcctg tcagcaaaga gacgacagtc ggtcggacaa cccttacctt tgacaacctt
    13261 aaacctggat tttatgacct taaagaaacg aaagcgccga atgcttacgt acttgatcct
    13321 aagacttatg ttgtggtcgt tcaaaattca ggaaaaacga caattgtgga tgaagctaac
    13381 ttcaaagagg ctgattaccc aatggctgat aataccagcc atgtggagtg cgtagcgttg
    13441 ctacaacgaa gcaaagggta aaaatccttt attttaagca ctttttcaag cattttgtct
    13501 ttattgaaaa gagtgatttt aacataaaaa aggtattaaa aaacatattg acgtgaccgt
    13561 ttgttttgaa gtggcttgcg tagacaaaaa aatagatacg tcagataaat ttctggcatt
    13621 acgagaacat ttttagagtg ttctcttttt ttagtttacg gaggaaaaat atatatggaa
    13681 aaacaggatt cacgagttct catccattgg gaggggaatt ctggggacaa gctcattgaa
    13741 caccaaacca gcgcaacggg gtggtactac caagtcgatc gtagctttag tcaaccaaaa
    13801 ggggaaccgc ccagaatgat ccaaaggcac tagaaagtgt ccgtaatgat tcgatttcgg
    13861 gcggtgatga tgtcatgggt tatgcttata gcaaatgtac ttggggagtt gcggcacgaa
    13921 ttaatcagtg ggactgaaac tcaaaggttg aaatggtgag aagattacca ttaccagttc
    13981 aatgggaaat ggtcaggatt gggttggaac agccgaaaga ctagatggtg aaactgatac
    14041 agttccaaaa gaaggtacta ttctctcttt ttaggaaagt agttatggtt cgtatatagg
    14101 ctacggaact atatctttcg tcacattaca tctacagata gtaccatgaa ttttgcttat
    14161 atgaccaagt aaagtgagga tatactaaca aatgaaatat ttattatcgt atttgtccat
    14221 tttatcgaaa agtttgcata ttatcattat gtttgataag atgcaaatat aatgatagta
    14281 ggagctaaat atggatattt aaaaaatcaa gagtatccta agtgctttcc attttgaaat
    14341 tcaaatatag ctaatagttc tagaacttct aattgttttt cgtcgacgat atgaattttc
    14401 aatcttaact gttaggattc cacctccctt tggttaaaga aaaaaggtca ggtcgtttag
    14461 ataactttgt caaacaagct caagctatct aaaaatagtt tgaaatgggc attactctag
    14521 tttttaataa gctatctgat gagcagaagg agaagttaat gcatgttggg aagtcttatt
    14581 ttgactatca agaaaatgct ctt
//
LOCUS       DATROD010000037        13872 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.037, whole genome shotgun sequence.
ACCESSION   DATROD010000037 DATROD010000000
VERSION     DATROD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13872
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.037"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(535..2130)
                     /gene="cls"
                     /locus_tag="VBC74_001074"
     CDS             complement(535..2130)
                     /gene="cls"
                     /locus_tag="VBC74_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEO6942030.1"
                     /translation="MIWRGEKLISNKVKIVRLLNKSKKSLLRGIFSRTTVIAILLILQ
                     LLFLLASYSWLEQYRVWLATVEHILTIGAVLYLVNSEMDALSRVTWLILVMIAPLLGA
                     MFLMYTKFDWGYRGLKQRLETLIDESQIYLEDDPETLNQLKSSTSTTYHLVQYFEKAH
                     GNFPVYRNTDVTFLPTGEAFFEKMKEELLKAKKYIFLEFFIIDEGIMWGEILSILEQK
                     VEEGVEVRILYDGMIEITKLSFDYTKRLEKIGIKAKAFSPISPFISTYYNYRDHRKIV
                     VIDGVVGMTGGVNLADEYINHIELFGHWKDSGIMLKGKAVDSFLLLFLQMWSITEEKM
                     LVAPYLGVHDDLVENEGYVIPYGDSPLDTDKVGENVYIDILNHAREYVYIMTPYLILD
                     SELEHAIQFAAERGVDVRIIMPGIPDKPIPYALAKTYYQALIKSGVKIYEYTPGFVHS
                     KIFLSDNTKAVVGTINLDYRSLYHHFECAVYLYKVDAIQDIYRDYMDTLNKSRLVSLK
                     DINNIPKFQKVIGIVTKTIAPLL"
     gene            complement(2249..3919)
                     /locus_tag="VBC74_001075"
     CDS             complement(2249..3919)
                     /locus_tag="VBC74_001075"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEO6942031.1"
                     /translation="MKTDIEIAQSVALKPIAEIVEQVGIGFDDIELYGKYKAKLSFDK
                     IEAVRSQKVGKLILVTAINPTPAGEGKSTMSIGLADALNKIGKKTMIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELDIDQRR
                     VIWKRVVDLNDRALRQVIVGLGSPVNGIPREDGFDITVASEIMAILCLATDLSDLKKR
                     LSNIVVAYSRDRKPIYVKDLKIEGALTLILKDAIKPNLVQTIYGTPALVHGGPFANIA
                     HGCNSVLATSTALRLADYVVTEAGFGADLGAEKFLDIKTPNLPTSPDAIVIVATLRAL
                     KMHGGVSKEDLSQENVEAVKRGFTNLERHVNNMRQYGVPVVVAINQFTADTESEIATL
                     KTLCSNIDVAVELASVWEDGADGGLELAQTVANVIETQSSNYKRLYNDEDTIEEKIKK
                     IVTKIYGGNKVHFGPKAQIQLKEFSDNGWDKMPICMAKTQYSFSDNPNLFGAPTDFDI
                     TVREFVPKTGAGFIVALTGDVLTMPGLPKKPAALNMDVLEDGTAVGLF"
     gene            complement(4008..5027)
                     /locus_tag="VBC74_001076"
     CDS             complement(4008..5027)
                     /locus_tag="VBC74_001076"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO6942032.1"
                     /translation="MYLIEPIRNGKRITDGAIALAMQVYILQNVFLDDDILFPYYCDP
                     KVEIGKFQNAVIETNQEYLKEHDIPVVRRDTGGGAVYVDSGAVNICYLMKDHGQFGDF
                     KRAYEPAIKALKTLGASSVEMRGRNDLVIDGKKVSGAAMTIVNGRIYGGYSLLLDVDF
                     DAMEKVLNPNRKKIESKGIKSVRSRVGDIRSHLSEDYRHITTDQFKDLMVCQLLHIDH
                     IDQAKRYHLTEKDWAAIDALADEKYKNWDWNYGNSPQYSYHRDARFPSGTYDFHLEIE
                     KGIITNCRIYGDFFSSKDISDIENLLIGCPMKEELVLEKLSTLSLEDYFGQTSPEEIK
                     AVLFS"
     gene            complement(5054..5932)
                     /locus_tag="VBC74_001077"
     CDS             complement(5054..5932)
                     /locus_tag="VBC74_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEO6942033.1"
                     /translation="MTVWKTLEKTNHSQSEILSQLIEESDAIVVGIGAGMSAADGFTY
                     IGPRFEEAFPDFIAKYQLLDMLQASLYDFEDWEEYWAFQSRFVALNYLDQPVGQAYLD
                     LKDILAKKEYHIITTNADNAFAVADYNLEKVFHIQGEYGLWQCSQHCHQQTYRNDQAI
                     RQMIAQQKDMKIPSNLIPKCPKCDQPFEINKRNEEKGMVEDADFHAQRQRYENFLSQH
                     QNDKVLYLEIGVGHTTPQFIKHPFWRFVSLNENSLFVTLNHKHYRIPQKIRSRSVQLT
                     QHIAELIAEAKKEIIG"
     gene            complement(5901..6719)
                     /locus_tag="VBC74_001078"
     CDS             complement(5901..6719)
                     /locus_tag="VBC74_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEO6942034.1"
                     /translation="MPNQKQLLLAMIEYLQSEKLTDVDDLRTTDLQTVWRGLVNQQDP
                     QNISQEYLSLEDRYLSHWWNTQKVKTIDVCHQTVYSNVFTYHGDICLLQVDAIVNAAN
                     SKLLGCFIPNHHCIDNQIHTFAGSRLRLACHQLMTQQGRMEAVGQAKLTESYHLPCKY
                     VIHTVGPYVKVDQKPSRIREDLLKSSYKSCLQLAAQANLKTIVFPCISTGEFGFPNQR
                     AAELAVQEILEWQRENQHKLYIIFNTFTPKDQEIYQKLLLKEEIDDSVEDSRKN"
     gene            complement(6712..7044)
                     /locus_tag="VBC74_001079"
     CDS             complement(6712..7044)
                     /locus_tag="VBC74_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEO6942035.1"
                     /translation="MKKIANYLLIEKNEELYTISLTPELQDDLGTVGYVEFTDDTNLE
                     VDDVILNIEASKTVMAILSPLTGKVVKVNTAASQEPTLLNSEKADENWLVVLTDVDYA
                     AFEALENA"
     gene            complement(7073..8059)
                     /locus_tag="VBC74_001080"
     CDS             complement(7073..8059)
                     /locus_tag="VBC74_001080"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HEO6942036.1"
                     /translation="MKLSVLDYGLIDYGKTASDAIQETILLSQEAEQLGYHQFWVAEH
                     HGVKAFSISNPELMIMHLANQTKSIKIGSGGIMPLHYSSFKLAETLKTLETCHPNRVS
                     IGLGNSLGTVKVSNALRSLHKAHDYEEVLEELKSWLIDESSSKEPLVQPTLSSFPDLY
                     VLGSGQKSAYLAAKLGLGFTFGVFPFMDKDPLTEAKKLSSLYYHQFEEYYPNKSPNLM
                     VAPFVVIADTSEEAENIAKTLDIWMLGNKDFNEFATFPTIEEANHYQLTPEQKAKIKS
                     NRHRMIVGDPKQVKESLDALVNASQAEELLLIPLVPGLDQRIKSLKLLSQLY"
     gene            complement(8056..9255)
                     /locus_tag="VBC74_001081"
     CDS             complement(8056..9255)
                     /locus_tag="VBC74_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEO6942037.1"
                     /translation="MNNVQGNLFRPLTLPNGLSLENRFVLSPMVTNSSTSEGFVTDDD
                     IAYAVRRAKSAPLQITGAAYITEYGQLFEYGFSVSKDEDIPGLTKLAKAMKSKGAKAV
                     LQLTHAGRFSSHTLARHGYVYGPSPMQLQSPYPHQVKELTHKDILRIIDEYVQATRRA
                     IQAGFDGVEISSAQRLLIQTFFSTFSNQRKDEYGPQTLTNRCRLGLEVFKAVQKVIRE
                     EAESDFILGFRATPEETRGSQIGYSIEEFMEFLEKILAIAQVDYLAIASWGHDVFRNT
                     IRSEGVYKGQLVNQVIFEHFGDRVPIMATGGINSASKVFEALQHAHMIGASTPLVVDP
                     EFLQKIKAKRSDQINLRIKVNDLEGLAIPKASFKDIVPLMDYGESLPKEAREVFRELR
                     SNYREER"
     gene            complement(9248..10084)
                     /locus_tag="VBC74_001082"
     CDS             complement(9248..10084)
                     /locus_tag="VBC74_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEO6942038.1"
                     /translation="MEWQDLAQLPVSIFKDYVTDAQDAEKPFIWTEVFLREINRSNQE
                     IILHIWPMTKTVILGMLDRELPHLELAKKEIISRGYEPVVRNFGGLAVVADEGILNFS
                     LVIPDVFERKLSISDGYLIMVDFIRSIFSDFYQPIEHFEVETSYCPGKFDLSINGKKF
                     AGLAQRRIKNGIAVSIYLSVCGDQKGRSQMISDFYKIGLGDTGSPIAYPNVDPEIMAN
                     LSDLLDCPMTVEDVIDRMLISLKQVGFNDRLLMIRPDLVAEFDRFQAKSMANKGMVSR
                     DE"
     gene            10223..10921
                     /gene="coaB"
                     /locus_tag="VBC74_001083"
     CDS             10223..10921
                     /gene="coaB"
                     /locus_tag="VBC74_001083"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEO6942039.1"
                     /translation="MYNMAMKILITSGGTTEKIDTVRSITNHATGALGKIIAEKYLRE
                     GHQVTLVTTKNAVKPESATNLSIFEIEDVDSLIKTLKPLVKEHDILIHSMAVSDYTPV
                     YMADFEKVKSSDDLDTFLRKDNHEGKISSESEYQVLFLKKTPKVISLVKKWNPQITLV
                     GFKLLVNVTKENLFKVARHSLIKNKATFILANDLIDITSKHHIAYLLDHDNVYKATTK
                     EDIAQLIYEKVKKI"
     gene            10918..11460
                     /gene="coaC"
                     /locus_tag="VBC74_001084"
     CDS             10918..11460
                     /gene="coaC"
                     /locus_tag="VBC74_001084"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEO6942040.1"
                     /translation="MIKRITLAVTGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNPIHLDVMDEHNPKIINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALAMPDETPKLIAPAMNTKMYHNTITQRNIDILKKIGYQEIEPRISLLACGDT
                     GQGALADISTILKCIQEVAN"
     gene            11506..12078
                     /locus_tag="VBC74_001085"
     CDS             11506..12078
                     /locus_tag="VBC74_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6942041.1"
                     /translation="MKKKNKSSNIAIIAIFFAIMLVIHFLSSFIFSFWLVPIKPTLMH
                     IPVIIASIAYGPRIGATLGALMGGISVANSSIVLLPTSYLFSPFVENGNFYSLIIALV
                     PRILIGIIPYFVYKLLHNRFGLAISGAIGSLTNTVFVLSGIFIFFSSTYNGNIKLMLA
                     GIISSNSLAEMVIAAIIVYLTVPRILNIKH"
     gene            12188..>13872
                     /locus_tag="VBC74_001086"
     CDS             12188..>13872
                     /locus_tag="VBC74_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO6942042.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKRGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSSGLIQVIGEDVDIEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVNADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMDKFRENGPKQFNNTDIVLLEDFQKQIATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATVGNDLADAETKIA"
BASE COUNT         4482 a         2689 c         2188 g         4513 t
ORIGIN      
        1 tgatatctaa aacatgatat ttttaaattt tcctttaata ttataaaaca tgatagatag
       61 tcatactaac attaggattt acaatgagat aatagtatat ttttaatact tcatctaaac
      121 catcagaaac tagattattt aaaaatttta caaagaatgt gatggtagca tattcaatta
      181 aagttaagta aaaacataat ttttagaaag aaattgtgac ttatttatgc aaaaaaaaga
      241 aagaaccaaa ctggttcttt cttttttgct tttcagctac ttaccacagt ttgtaatgaa
      301 ttacaagtag tagtaagcta agggcacctt ttcaagtgtg ataagaaggt tcacacaact
      361 tatcaagatc cgctcctgcg tttataatct ccctgcgcaa tagtggtaaa accacatatc
      421 gactcatcgc ataacagtag tttaacataa tttagttaaa atgcaacctt tactttttaa
      481 atataactag attcttcagt ttacttttaa tattaaatta aaaataaatt atttctaaag
      541 caggggagca atagtttttg tcactatgcc aattactttt tggaattttg gaatattatt
      601 tatatctttc aagctaacta accttgattt atttaacgta tccatataat ctcgataaat
      661 gtcttggatt gcatctacct tataaagata aacagcacat tcaaaatggt gatacaggct
      721 acggtaatca agatttatcg tacctacaac agcctttgta ttatcactaa ggaatatttt
      781 tgaatgaaca aatccagggg tgtattcata tattttaaca cctgatttta ttaatgcttg
      841 atagtaagtt ttagcaagag cataagggat tggcttatct gggatacccg gcataataat
      901 acgtacatct acaccacgtt ccgcagcaaa ctgaatggca tgttccaatt cactgtctaa
      961 tattaaataa ggtgtcatga tgtaaacata ttctctagca tgattaagaa tatctatata
     1021 aacattttcc cctactttat cagtgtctaa aggagaatct ccataaggaa taacatagcc
     1081 ttcattttca actaaatcat cgtgtacacc gaggtaaggc gctactaaca ttttttcctc
     1141 agtaatggac cacatctgta agaaaagtag gaggaaacta tccactgctt tcccctttaa
     1201 cataatacct gaatctttcc aatgcccaaa taactcaatg tgattaatat actcatcggc
     1261 taagtttaca ccaccagtca tgcctactac tccatcaata acaacaattt ttcgatggtc
     1321 acgatagtta taataagttg aaataaaagg agaaatcgga gaaaatgctt ttgcttttat
     1381 tcctattttc tcaagtcgtt tcgtataatc aaaggataat tttgtaattt ctatcatacc
     1441 atcatataag atacggactt caacaccttc ttccaccttt tgttctaaaa tactaagtat
     1501 ttctccccac attattcctt cgtcaatgat aaagaattct aaaaagatat acttcttagc
     1561 ttttagtagt tcttctttca ttttttcaaa gaatgcttct cccgttggta gaaatgtcac
     1621 atcagtattt cgataaactg ggaaatttcc atgtgctttt tcaaaatatt gaaccaaatg
     1681 atatgttgtg gatgtactcg acttaagctg attcaaagtt tcaggatcat cctctagata
     1741 aatctgactc tcatcaatca atgtctccaa tctttgtttc aaaccacgat aaccccagtc
     1801 gaatttggtg tacattagga acatcgcccc tagtaaaggg gcaatcatta ccaatattaa
     1861 ccaagttact cgggacaaag catccatttc actattaact aaatataaga ccgctccaat
     1921 tgttaagata tgctcaacgg ttgctaacca aacacgatac tgctctagcc atgagtaaga
     1981 ggctagtaaa aacaataatt gtaaaatcaa taaaatagcg ataacggttg ttcgactaaa
     2041 tatacccctt agtaaacttt tcttactctt attaagtaaa cgaacaattt tgactttatt
     2101 actgataagt ttttcccccc tccaaatcaa tcattttatt atagcatact gtcacttgaa
     2161 tagcattcta ttattctaaa ttatctctag ttaaaatcta atccccaata aaaagaggct
     2221 agcatcacaa gccctacctc tttattattt aaaataatcc aacggctgta ccgtcttcta
     2281 aaacatccat attgagagct gcaggttttt taggtaaacc aggcattgtc aatacgtctc
     2341 cagtaagagc aactataaaa ccagctcctg tttttggaac aaattcacga acagttatat
     2401 caaagtcagt tggagcacca aataaatttg gattatcaga aaagctatat tgtgtctttg
     2461 ccatacaaat aggcatcttg tcccagccat tgtcactaaa ctcttttaat tgtatttgtg
     2521 ccttaggtcc aaaatgaact ttattaccac catatatttt agtaacaatt tttttaattt
     2581 tttcttcaat agtgtcttca tcattatata agcgtttgta attggatgat tgtgtttcaa
     2641 tcacattagc aacagtctgt gcaagttcaa ggccaccgtc tgctccatct tcccacacac
     2701 ttgctaattc aactgccaca tcaatattac tacataaggt tttaagagtt gcaatttcac
     2761 tttctgtatc tgcagtaaac tgattgatag ctacaactac cggaacacca tattgacgca
     2821 tattgttaac gtgacgctcg agatttgtaa aacctctttt aactgcttcg acgttctctt
     2881 gtgagagatc ttcttttgaa acacctccat gcattttcaa tgcacgcaat gtagcaacaa
     2941 taacaatcgc atcgggagaa gtcggaaggt ttggcgtttt tatatcaaga aacttttcag
     3001 cgcccaagtc tgctccaaaa ccagcttcag taacaacgta atctgctaag cgtagagctg
     3061 ttgaagtcgc taagacagaa ttacatccat gtgcaatatt agcaaatggt cccccatgga
     3121 caagagccgg tgtcccataa atggtttgaa ccaaattagg tttgatagca tcttttagga
     3181 taagtgtgag agctccttca atcttcaaat ctttaacata gattggttta cggtcccttg
     3241 agtaagccac aacaatattt gacaggcgtt tctttaaatc tgataggtca gtagctaaac
     3301 atagaatagc cattatttca gaagcaacag taatatcaaa gccatcttca cgtggtatcc
     3361 cattgacagg gcttcctaag ccaacgatca cctgacgaag agcacgatca ttaagatcaa
     3421 ctacacgctt ccaaatcact cgacgttggt caatatctaa ttcattgcct tgatgaatat
     3481 gattatcaag tagagctgat aaagcattat tagcagttgt gattgcgtgc atatcaccag
     3541 taaaatgcaa attaatatct tccattggta gtacttgtgc gtatccacct ccagcagctc
     3601 ctcctttaat acccattaca ggacctaaag aaggttcgcg aagcgcaatc atcgtttttt
     3661 taccaatttt atttaaagca tcagctaatc caatgctcat agtggatttt ccctcacctg
     3721 ctggtgtcgg attaatagca gttactaaaa ttaactttcc tactttttga gatcttacag
     3781 cttcaatctt atcaaaagat aattttgctt tgtatttacc atacaattct atatcatcaa
     3841 aaccaattcc tacttgctca acaatctcag caataggctt tagggcgacg ctttgcgcta
     3901 tttcaatatc tgttttcatg atattctcct tctaaataat aaaaatagta tagcaatatt
     3961 ccctataaaa acatattttt ttgcaaaaaa aatttaaaat gttcgtctta tgaaaataaa
     4021 actgctttta tttcttctgg actagtctga ccaaaataat cttctaatga taaagtactt
     4081 aatttttcca agactaattc ttctttcata gggcaaccaa tgagaaggtt ttcaatatct
     4141 gaaatatctt tagaactgaa aaagtcacca taaatacggc aattcgtaat aatacctttc
     4201 tcaatttcta agtgaaaatc gtaagttcct gacggaaaac gagcatctct gtgatacgaa
     4261 tactgtggtg agttaccata attccaatcc caattcttat atttttcatc agctagagcg
     4321 tcaatcgcag cccaatcttt ttcggttaga tgatagcgtt ttgcctgatc aatatgatca
     4381 atatgtaata attgacaaac cattaaatct ttaaattgat cggttgtgat atgacgataa
     4441 tcttctgaca agtggctacg aatatctcct actcgacttc ggacagattt tatcccttta
     4501 gattcaattt ttttccgatt aggatttaac actttttcca tagcatcaaa atcaacatct
     4561 aataaaagag agtaaccacc gtaaatacga ccgtttacta tcgtcattgc tgcacctgat
     4621 acttttttac catcaataac taagtcattt cgtcccctca tttcaacact agaagcacct
     4681 aaagttttta gggccttaat agctggctca taagctcgtt tgaaatcacc aaattgtcca
     4741 tgatctttca ttagatagca aatatttaca gcacctgagt caacgtaaac agctcctcca
     4801 ccagtatctc gacgaacaac aggaatatca tgttctttca aatattcttg atttgtctct
     4861 ataactgcat tctgaaattt tccgatttca actttaggat cacaatagta tggaaataaa
     4921 atatcatcat ctaaaaaaac attttgtaaa atataaactt gcatggcaag tgcaatcgca
     4981 ccgtctgtga tacgcttacc atttctaatt ggttcaataa gatacattta ggtctccttt
     5041 tatagcaatt tagttatcca ataatttctt ttttcgcttc agcaattaat tcagcgatat
     5101 gctgtgtcag ttgcacactc cttgagcgaa tcttttgagg aatgcgataa tgcttgtgat
     5161 taagagtaac aaataaagag ttttcattta aagagacgaa tcgccaaaac ggatgcttaa
     5221 taaattgtgg cgtggtatgt ccaactccaa tctctagata aagaactttg tcattttgat
     5281 gttgtgataa gaaattttca taacgttgtc gttgagcatg aaagtcagca tcttccacca
     5341 tacctttttc ttcgttacgt ttattaattt caaacggttg atcacacttt gggcatttgg
     5401 ggattaagtt actaggaatc ttcatatcct tttgttgtgc aatcatttga cgaattgcct
     5461 gatcatttcg ataagtttgt tgatgacaat gttgactaca ctgccataag ccatattcac
     5521 cttgaatatg aaaaactttt tctaaattat aatcagccac agcgaaggca ttatcagcat
     5581 tagttgtaat aatatgatat tcttttttcg ctaaaatatc ctttaaatca agataagctt
     5641 gtcctaccgg ttgatctaaa taattaagtg ccacaaaacg actttggaag gcccaatatt
     5701 cttcccaatc ttcaaagtca taaagacttg cttgcaacat atctaatagt tgatatttag
     5761 caataaaatc cggaaaagct tcttcaaatc tggggccaat ataggtaaat ccatccgctg
     5821 cagacatgcc tgcaccaata ccaactacaa tagcatctga ttcttctatt aactgactta
     5881 gtatttctga ttgagaatga ttagtttttt ctagagtctt ccacactgtc atctatttcc
     5941 tcttttaata ataatttttg ataaatctct tggtctttag gtgtgaatgt attgaaaata
     6001 atatagagtt tgtgctgatt ttcgcgttgc cactctaata tctcttgaac agccaattca
     6061 gcggctcttt gattaggaaa accaaattct cccgttgata tacaagggaa gacaattgtt
     6121 ttaagattag cttgagctgc caattgtaag caggatttat aagaagactt taataaatcc
     6181 tctcttatac gagatggctt ttgatcaact tttacataag gtccgactgt atgaataaca
     6241 tacttacaag gaagatgata ggattcggta agtttagctt gtcctacggc ttccattcgt
     6301 ccttgttgtg tcatcaattg atgacatgct aaacgtaatc gtgaacctgc aaacgtatga
     6361 atctgattgt caatacaatg gtgattagga ataaaacaac ctaacaactt actatttgct
     6421 gcattaacaa tagcatccac ttgtaataag caaatatccc catggtaggt aaatacattt
     6481 gaatataccg tttgatgaca gacgtctatc gtttttactt tttgagtatt ccaccaatgt
     6541 gacaaatacc tatcttctaa agacaggtac tcttgactaa tgttttgagg atcttgttga
     6601 ttaactagac cacgccaaac cgtttgcaaa tcggtggtcc tcaagtcatc aacgtctgtt
     6661 aatttttctg actgaagata ctcaatcatg gctagcaata gttgtttttg attaggcatt
     6721 ttctaaagct tcaaaagcag cataatcaac atctgttaac acaaccaacc aattttcgtc
     6781 agctttttca gaatttaaca aagttggttc ttgacttgca gctgtattaa ctttaacaac
     6841 tttacctgta agaggagata aaattgccat aaccgtcttt gaagcttcaa tatttaaaat
     6901 aacatcatca acttctaaat ttgtatcatc tgtgaattcc acatatccaa cagttcccaa
     6961 gtcatcttga agttcgggag ttaagctgat agtataaagt tcttcatttt tttcaattaa
     7021 taagtagttc gcaatttttt tcatgagtaa tttcctttct gagaaaacgt gattaatata
     7081 actgtgataa taattttaag gatttgattc gttgatcaag tcctggaaca agaggaatca
     7141 aaagtagttc ttctgcttga ctagcattaa ctaaagcgtc aaggctttct ttaacttgtt
     7201 ttggatcacc tacaatcata cggtggcgat ttgatttaat ttttgctttt tgttcaggag
     7261 tcaattggta atgattagct tcttcaatag taggaaatgt tgcaaattca ttgaagtctt
     7321 tatttcctag catccagata tctaaagtct ttgctatgtt ttcagcttct tctgaagtat
     7381 cagctataac tacaaatgga gctaccatca aattaggaga cttattagga taatattctt
     7441 caaactgatg ataataaaga gatgaaagct ttttagcttc tgtcaatggg tctttgtcca
     7501 taaaaggaaa aacaccgaag gtaaagccta agccaagttt agccgctaaa taagctgatt
     7561 tttgaccact ccccaacaca tataagtctg ggaagctaga aagagtcggt tgaactaatg
     7621 gttccttact ggatgattca tcaataagcc atgacttcaa ttcctccagt acctcttcgt
     7681 aatcatgtgc tttatgtaag ctacgaagtg catttgaaac tttaactgtc cctagtgaat
     7741 ttcctaaacc aatacttact cgattcggat gacatgtctc taatgtcttg agagtctccg
     7801 cgagtttaaa actactatag tgcagaggca ttatacctcc agagccaatt ttgatagatt
     7861 tagtctggtt agccaaatgc attatcatta attctggatt gctaatactg aatgccttaa
     7921 caccgtgatg ttcagccacc caaaattgat gatagcctag ttgctccgcc tcttgtgata
     7981 aaagaatcgt ttcttgtatt gcatcacttg cagtttttcc ataatcaata agcccataat
     8041 caaggacact taatttcatc tttcctccct ataattgctt cgaagttctc taaaaacttc
     8101 tcgagcttcc tttggtaaag attctccata atccatcaaa ggaacaatat ctttaaagga
     8161 ggcttttgga atcgccaaac cttctaaatc attcacttta atccttaaat taatctgatc
     8221 agaacgctta gcttttattt tttgtaaaaa ttccggatca actactaagg gcgtggaagc
     8281 tcctatcata tgagcatgtt gtaaggcttc aaacaccttt gaagcactat taatacctcc
     8341 agttgccata attggaactc tatcaccaaa atgttcaaat ataacttgat taaccagctg
     8401 ccctttgtaa actccctcag aacgtatagt atttctaaag acatcatgcc cccagcttgc
     8461 aatggcaaga taatctacct gagcaatcgc taaaattttt tcaagaaatt ccataaactc
     8521 ttcaatgcta tatccaattt gtgaaccgcg agtttcttca ggcgtcgctc taaaaccaag
     8581 gataaaatct gactccgctt cttctctgat aactttttgt accgctttaa aaacttctaa
     8641 gcctaaacga cagcgattag tcaaagtttg aggaccatac tcatcttttc tttgatttga
     8701 gaaggttgag aaaaaagttt gaattagtaa tcgttgtgca gaagatattt caacaccatc
     8761 aaatccagct tgtatagctc gtcttgtagc ctggacatat tcatcgataa tccttagtat
     8821 atctttgtgt gtcagttctt taacctgatg tgggtatggc gattgcaatt gcatagggct
     8881 gggaccatat acatatccgt gtcttgccaa agtgtgactt gagaaacgtc cagcatgcgt
     8941 cagctgcaaa acagctttcg ctcccttaga cttcattgct ttagccaatt tagtcaaacc
     9001 aggaatgtcc tcatcctttg aaacgctaaa accatattca aataactgac catattctgt
     9061 gatatatgct gctccagtaa tttgtaatgg agcggattta gcacgacgaa cagcatatgc
     9121 tatgtcgtca tcagttacaa aaccttctga tgtagaagaa ttagttacca taggtgatag
     9181 aacaaaacga ttttctaagc tcaatccatt gggaagtgtt aatggacgaa ataaattacc
     9241 ttggacatta ttcatctctg ctcaccatcc ccttattagc catagactta gcctgaaatc
     9301 tatcaaactc tgcaactaaa tcgggtctaa tcatcagtaa acgatcatta aaacctactt
     9361 gtttaaggct aatcaacata cgatcaataa catcttctac tgtcatagga caatctaata
     9421 gatcagatag attagccata atttcaggat ctacatttgg ataagcaatt ggactacccg
     9481 tatcacctag accaatctta taaaaatctg aaatcatttg actccgccct ttttgatcgc
     9541 cacaaacgct aaggtaaatt gataccgcaa taccattctt tatacggcgc tgagccaagc
     9601 cagcaaattt tttgccattt atactaagat caaacttacc aggacaatag gaggtctcta
     9661 cttcaaagtg ctcaataggt tgataaaaat ccgaaaatat acttctaata aaatcgacca
     9721 ttataagata cccatctgag atagacaatt ttctctcaaa aacatctgga ataaccaatg
     9781 aaaaatttaa aattccttca tcagctacaa ctgcgagacc tccaaaattc cgaacaactg
     9841 gttcataacc acgactgatg atttcttttt tagctaattc taaatgtggt aattctcgat
     9901 ctaacatccc cagaatgact gtcttagtca tcggccaaat atgcaaaata atttcttgat
     9961 ttgagcgatt aatctccctt aaaaatactt ctgtccatat aaaaggtttt tccgcgtctt
    10021 gagcatctgt aacatagtct ttaaaaatag atacaggtaa ctgcgctaga tcttgccact
    10081 ccaataaatc tgacctcctt tctctagaaa actctgcaag atttatttta tcgcaatcgc
    10141 ttgaaatttt ccagtaagtt tccctcaaat tccattacta tcaagaagaa atatatcttt
    10201 tatttacgaa aagcattttt ttatgtacaa tatggctatg aaaattttga ttacatcagg
    10261 tggaactacc gagaaaattg ataccgttag gagtatcacg aatcacgcaa caggagcttt
    10321 aggaaaaata attgcagaaa aatatttacg agaaggacat caggtgacac tcgtgactac
    10381 taaaaatgca gtcaaaccag aatctgctac caacttatcc atatttgaga ttgaggacgt
    10441 tgactcatta ataaaaacct tgaagccatt agtgaaagag catgatattt taattcatag
    10501 catggcagtg tctgactata cccccgtcta catggctgat ttcgaaaagg ttaaatcaag
    10561 cgatgattta gatacttttt tgcgcaaaga taaccacgaa gggaaaatat catctgaatc
    10621 agaatatcaa gtcctttttc tcaaaaaaac accaaaagtt atcagccttg tgaaaaaatg
    10681 gaatcctcaa attactctag taggttttaa actacttgtg aacgttacta aagaaaacct
    10741 ttttaaagtt gctcgtcata gcttaatcaa aaataaagct acgtttattt tagctaatga
    10801 cctcattgat attacctcaa aacatcacat agcctaccta cttgatcatg ataatgtgta
    10861 taaagctacc accaaagaag atattgctca attaatttac gagaaggtaa aaaaaatatg
    10921 attaaacgta ttacactagc cgtcacaggt tcaatttcgg cctataaagc agcagacctt
    10981 acaagtcaat taactaaaat tggctatgat gttcatatca taatgacaca agctgccaca
    11041 caatttatta caccgttaac tctgcaagta ttatcaaaaa atcctattca tttagatgtt
    11101 atggatgagc ataacccaaa aatcatcaac catatcgaat tagcaaaacg cacagattta
    11161 tttatcgttg cacctgcctc tgctaatact attgctcatc tagcatatgg cttcgctgat
    11221 aacatcgtaa caagcgttgc tcttgctatg cccgatgaaa ctccaaaact tatcgcacca
    11281 gcaatgaata caaagatgta tcataataca atcacccaac gtaacattga tattttgaaa
    11341 aaaataggtt atcaggaaat tgaacctcgt attagtttgc ttgcctgtgg tgataccggt
    11401 caaggagccc tagctgatat ctctactata cttaaatgta tacaagaagt tgcaaattag
    11461 gctgtaaatt tgtatactat ttctagttta atttaaggag ttataatgaa aaagaaaaac
    11521 aaatcctcta acattgctat aattgcaatc ttttttgcta ttatgcttgt cattcatttt
    11581 ttgtcatcat ttatttttag tttttggtta gtccctatta aacctacttt gatgcatatc
    11641 ccagttatta ttgcatctat agcctatgga cctcgtattg gtgcaactct aggcgcctta
    11701 atggggggga tcagcgtagc taacagcagc attgttctat taccaacgag ttacctcttc
    11761 tcaccttttg ttgaaaatgg taatttttat tcgctaatta ttgcacttgt accacgtatt
    11821 ctaatcggga ttattcctta tttcgtttac aaattactac acaaccgctt tggtttggct
    11881 atctcaggtg ctataggctc tctaacaaac acagtatttg ttttatctgg aatttttatc
    11941 tttttttcaa gtacttataa tgggaatatc aagctaatgc tcgctgggat tatttcatct
    12001 aattcattag ctgagatggt cattgcagct atcattgtat atctaactgt tcctcgtatt
    12061 ctcaatatta aacattaaaa actggccagg aagaaatctt cctggctttt cttacttata
    12121 ttttatagtc atattgaaaa aaacatgtta taatgaaagc gtttataaat attgaaggag
    12181 tatttcaatg acttacacag aaaattttca aaaatggtta gattttgagc agcttcctga
    12241 ctatcttcgt caagaattac tatcaatgga tgaaaaaaca aaagaagatg ctttttacac
    12301 aaacttagaa tttggtactg ctggtatgcg tggttatatt ggtgctggta caaatcgtat
    12361 taatatttat gttgtccgtc aagcaaccga aggattggca aaattaatcg aaactaaggg
    12421 agaggaagct aaaaaacgtg gtgtagccat tgcttatgat tcacgccact tctctccaga
    12481 atttgctttt gagtctgcac aggtcttggc acagcatgga attaaatctt acgtatttga
    12541 agctcttcgt ccaacaccag aactctcttt tgctgttcgc catttaaatg cctatgctgg
    12601 tattatggtt actgcaagcc acaatccagc acctttcaat ggttataaag tctatggaca
    12661 agacggaggt caactaccac cagctgatgc tgatgcacta acagacttta tccgtgctat
    12721 tgaaaatcca tttgcagttg aacttgctga tttagatgaa agcaaatcat cgggccttat
    12781 tcaggttatc ggtgaagatg ttgatattga atatcttaga gaggttaaag atgttaatat
    12841 caatcaagat ttaatcaata actttggaaa agacatgaaa attgtctata ctcctctcca
    12901 cggaactggt gagatgttga cacgccgtgc ccttgctcaa gccggttttg aatctgttgt
    12961 tgtagtagaa tcacaagcta aggcagatcc agacttttct actgttaaat cgcctaaccc
    13021 tgaaagccaa gctgcctttg ctcttgctga agaactcgga cgagaagtta atgcagatgt
    13081 cttagtagct actgatcctg atgcagaccg cctaggcgtt gaaattcgcc aaccagatgg
    13141 ttcttataaa aacctttctg gaaatcaaat tggagcgatt attgcgaaat acatcctgga
    13201 agctcataaa acagcaggaa cacttccaga aaatgctgct ctcgctaaat caatcgtatc
    13261 aacagaatta gtaacaaaaa ttgccgaaag ttatggcgct acaatgttta acgttttaac
    13321 tggattcaaa ttcatcgctg agaaaattca agagtttgaa gaaaaacata accacaccta
    13381 tatgtttggt tttgaagaga gcttcggata tcttattaaa ccatttgtcc gtgataaaga
    13441 cgctattcaa gcagttcttt tggttgctga gattgcagct tattaccgtt cgcgtggttt
    13501 aacacttgca gatggtattg atgaaattta caaagaatat ggttattttg ctgaaaaaac
    13561 gatttcagta acactttcag gagtggatgg agctgctgaa attaagaaga ttatggacaa
    13621 attccgtgaa aatggtccaa aacaatttaa caatactgat attgtattat tagaagattt
    13681 ccaaaaacaa atcgctacta aaaatgatgg aacaatatct aacttaacaa ctcctccaag
    13741 taatgttttg aagtacacgt tagctgatga ttcatggatt gcagttcgcc cttcaggaac
    13801 tgaaccaaaa atcaagttct atattgctac ggtcggtaat gatttagcag atgcagaaac
    13861 aaaaatcgcc aa
//
LOCUS       DATROD010000038        13766 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.038, whole genome shotgun sequence.
ACCESSION   DATROD010000038 DATROD010000000
VERSION     DATROD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13766
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.038"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            4..459
                     /locus_tag="VBC74_001087"
     CDS             4..459
                     /locus_tag="VBC74_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942043.1"
                     /translation="MELTVAKRSIEIKFDFKTMFKINNRLGTVNKETGERNADGVGTL
                     FYKILERDDSAVVDLVKLSVGSGKKALSEDEVLDSIAELVEEEGSTEALFKEIENDMV
                     ESGFFKEKISKYIESMEKSVKYLEAREDADEAQIQIVKDMIGKMKDAIS"
     gene            534..764
                     /locus_tag="VBC74_001088"
     CDS             534..764
                     /locus_tag="VBC74_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942044.1"
                     /translation="MEGLQYKQIAERENLSELALNLRYTLNAKNVNANKLKLDKQRNK
                     IKRTYQNKQDLTNVSNDFAERVAKLNLHFQNL"
     gene            783..5009
                     /locus_tag="VBC74_001089"
     CDS             783..5009
                     /locus_tag="VBC74_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEO6942045.1"
                     /translation="MAGTFDGSIFADIGANTKDYEAAMARIVSTTQNAFRKAQDTAVN
                     NSNKMIQMIGQIMAQLANNGQSLGQRLGNAYSTGLKLSLGQIQRIAASIGEKIPEPIK
                     NGFNKAFSIIQNSVQKLSSAIPQPIKSAFTSATSAVVSFSSKITSAVSAAFNSVSSKA
                     STVADKISSSFGGRITSAVTNLATKLSTGLSNGFSRMSSSATSALNGISQRFASASSA
                     GEKLRSTVGSIISAFSLMAIARKGIQAITGAMDGAISRVDTMNRFPKTMALFGYSAQQ
                     SKASIDKLSKGIEGLPTPLDSAVKSAQQLAITTGSLDKGTELALAFNNAMIGYGATTE
                     GAEQALRQFNQSLGSGKIYAEEFNSVSEAAPGLMSKMAESFGFGKNGVQDLKAALSDG
                     SITAQEFADKMIELNKAQGGFAEMAQSSAGGIRTAWKNVHTAVVKGVAGMISAFDEAA
                     KANGMKTIAESINSIKPAINSAFATVNSIMPNLVAAFARISKMVNIDFSPLTSGIKSA
                     FGLLNTALGEFAKTGEVSSATIDRIKSKIASIGPTAIAAWALLNPASAIATIMPLISI
                     IGKIGVALGTVGSALGSFGEMVASGLSTASGLVGAFGRAMSGLPGIFGVAASRGLAVL
                     DGMTTAISSAAILAMSAIGPAAILGLVVVGLGLVNNQFGTQIDKLINTAITKGPGIIQ
                     GLVKGITSKIPALIASGTQLIAKFANALTVLLPVIIQAGVQLITSLVQGIGQNAGSLI
                     SSALKVIGTFVSSIASALPQILAVGVQLILNLARGIVQNIPLIIQYAQQIISNFGSSL
                     QANMPSIISNGIAISTNLNAGIIQMLPAVIQIATQVITGFISGIAQYLPQVIQGGIQI
                     IIMLVQGIIQNLPQVIQAAMQIIQSLISGLSQALPQIISAGIQLVVQLAVAIIKGLPQ
                     IISAGIQLIAGLGKAMLEAIPNALSGVWEGIKGGFSSMWDQITGKSSTSAAKVTSDMS
                     TMTMNVGMQTTTMANQTNIDTTTMLNSISQNTGLANLNATTNATQMATNVNAQTASMA
                     NQGLLNAAGFNEALSSNMTLANTNATNQANQMTANVNNATSQMSLGSLPNFLELSQQA
                     TSNMQTAQANATTSANGMSSGVTGATAGMASGVANNMLGITSTVSTGMNQAANAAKSG
                     GANISNSVNSSFTQAKSTVSSSMSGMNSTVTSGMNSINNTVKSGGNNIKSTFTSSFNS
                     AKSTVSNAMSSIRSSISSGMNSAVSVTRSAMNSVVSAMNAAASGARSAGYNTGIGFAN
                     GLASTAGTIMAIANSIAANVAATIRSAMDIHSPSRVTRELGAYTGEGFALGMQDWMDD
                     INAIGKEYANAVTDQRWETDSQLATSANISSAGVSSSLDNLSDEVRNQQLTSPTFEVH
                     NELVGDEIYTTIKSKEARESNKDNYFNFAT"
     gene            5022..5864
                     /locus_tag="VBC74_001090"
     CDS             5022..5864
                     /locus_tag="VBC74_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEO6942046.1"
                     /translation="MDLLVQKSDQSFRFSEIGLRILDVDDRSSSLEVDSRTVKGRSGK
                     IFASAKYGTKKVKVNGRLVVASIKDFMTKKDDVNGLLVDSEPFYITKMYPQKENLYDF
                     ELPGMKAGDLDLLNQSYTAWHYRWKVYVSSEPEYTFVGKSSEGLKYNFTIAFETAELP
                     FGETIAQNIVITNGTFNYSGTAPFSQLEYPWSVELTSTGGQSSFYLEIDGRRWAYQYS
                     APLNQGDVIVITGISTTLNKINVTARTNHEYFVLKQKVNKTILYSTNFQGTIKFLNFK
                     ELYK"
     gene            5877..9704
                     /locus_tag="VBC74_001091"
     CDS             5877..9704
                     /locus_tag="VBC74_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6942047.1"
                     /translation="MTTFLDHKDNEFEAQAVVKTTNAVNGERSLSGTIYTNEEVLNKV
                     DKGWKLEFDDEYYKIIYAKPTDTGNKIQVEFDAVHQFFYDFDKSSLHKQLNDGSHTFQ
                     AYLNFIFDGSGYTYSLEVVVKSFEKQSFGYKSRLDLFNDIIKSAGVEFFVRGKVVRIL
                     QKTGTDLSTIVRKNFNMNEIIIEKKINDFITYQKGFGAWADQEDHSKGRLEVEYESPL
                     AKEYGRIEGEPVVDERYTNADNLKSVLKQNVDSSYNIAVKIDIEDLTKAGYQYTQPIA
                     GDYIMAINETLGFKEKIRIVSFESEYDVTGQLINHKVTCNDIGTIKKQTVAVSQLSSK
                     INNNQAILDNAVFKANQALASADGKNTNYYGTEMPVDNPKGTLRKGDLLFLTVGDTTR
                     MYFWNGAEWIINSFSDDIDFVKKEITNKIIEVNAAMKLADEQLTEKYNAIVAKNVSQD
                     ELINQAKTLSDSAKKEATEALTNLISESKKLTDQMSALSKTEVEHYSTTNQQLTQIDG
                     TVKGLQTSYEALLKKDGDITQALANYKQTIDQNSASIMTNKKTADNALSSLQTQVIQT
                     SNEITTRLSQTNIEALITSKSAVIADNKVKETADSFSRELTRVNNTIDNLKIGTVNLL
                     SGTKGWTKNWFNRNNWQADDDSLSIGNFNLTVLKRKMMWSGISQSYAVKSGESYVFSA
                     YAKSSIDNDVVNLYLLPNDQSQQAKISISFKSITLNTDWQRIVIKFNVNSDGYILPRF
                     ERFNENGWLYIAGYQLEHGNVTSDYSESPADQEDKMTVEFNKINDTVNSHSQLIGKQN
                     ESLTATIQKVDNIQSTVSNVDGRLSRVTQTADGIVATVSQLDNNILPGTKKFTGWQTE
                     GAVLEELTQSPYPFVFKKWVSGNKVSPLIEYDVKKDQEYTFTAYISREQAGNLYFYLY
                     DLWNHHITSTTPRETIIKDVTQTIRRFKITFIPTRDGKIRPRFAMLASDPGSFSTGGF
                     MLSKGTVELEWSESKQDIKDSVTAINTTVTQTANGWAIKNLTSSGTVLSQLNLTDGNV
                     KLEGKLIHLSGQSIIDNGIITNAMIKDLTADKITAGTIDANRINVINLDASNITSGRM
                     SADLIRSGVLISQNGAMLADLNTGQIEFYTDSPAIKRITAGYPNQFVKFATGNVEGKG
                     AAGVTVIGSNRWGSESSNDGGFVGIRAWNGKDIDTLDLVGDSIHLASSAYTNADGWEI
                     ITLPNQLSIDARNINHRVTSKIKVGDIWLWKNTSTYTSMKDTINMIIDNLQLLHNNKT
                     TEKGYSYTIPGKI"
     gene            9713..9865
                     /locus_tag="VBC74_001092"
     CDS             9713..9865
                     /locus_tag="VBC74_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942048.1"
                     /translation="MEEKIKSNLALQIAQLSLDKATLQAQNEQLQLQIAELNQQLDEI
                     TKPGAK"
     gene            9876..10292
                     /locus_tag="VBC74_001093"
     CDS             9876..10292
                     /locus_tag="VBC74_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1366 domain-containing protein"
                     /protein_id="HEO6942049.1"
                     /translation="MKQWNIVGKPFPIYQDGVVLKTDVRLQGDDGTYLPQELIGNHVD
                     KDNQSLIKLVLETFAKENVVDFAMLESVKDIEQLKADKVTLIKKIAEADEAISKMSQA
                     TKDMQLKVDKAVVELTDLVTSSVSSLSVPSTSMESS"
     gene            10355..10516
                     /locus_tag="VBC74_001094"
     CDS             10355..10516
                     /locus_tag="VBC74_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942050.1"
                     /translation="MTYTFKKDHLIVDVWYRRVANGICDFEAVPKLFNLRDVVLELLS
                     QKVTDKESE"
     gene            10526..10816
                     /locus_tag="VBC74_001095"
     CDS             10526..10816
                     /locus_tag="VBC74_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942051.1"
                     /translation="MIDFVQVGAFAGAALSILGVWGFIVNPFKKAMEANEIAMAQLKD
                     SLKELAYELKNIDRDREITKAVIDRHEERLGRVEDDIIINKEQIKTLFNKEK"
     gene            10818..11069
                     /locus_tag="VBC74_001096"
     CDS             10818..11069
                     /locus_tag="VBC74_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEO6942052.1"
                     /translation="MINWKVRIKNKAFWSAIIPAIFLVVQAVANVFGYTLELSDLGNK
                     LLVVVNSGFSVLVIAGIVTDPTTQGVSDSSRALSYTEPK"
     gene            11199..12542
                     /locus_tag="VBC74_001097"
     CDS             11199..12542
                     /locus_tag="VBC74_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysin"
                     /protein_id="HEO6942053.1"
                     /translation="MATYQEYKSRSNGNAYDIDGSFGAQCWDGYADYCGFLGVPYANC
                     TNTGYARDIWEQRHENGILNYFGEVETMQAGDVAIFMVVDGVTPYSHVAIFDSDAGGG
                     YGWFLGQNQGGPNGAYNLVKIPYSATYPTAFRPKVFKNAVTVTGNTGLNKGDYFIDVS
                     AYQQADLTTTCQQAGTTKTIIKVSESIAWLSDRHQQQANTSDPIGYYHFGRFGGDSAL
                     AQREADLFLSNLPSKKVSYLVIDYEDSASADKQANTNAVIAFMDKIASAGYKPVYYSY
                     KPFTLNNVDYQQIIAKYPNSIWIAGYPDYEVRSEPLWDYFPSMDGVRWWQFTSVGVAG
                     GLDKNIVLLADDSSKVDIPKIDKPQEPQSQLTFNQKLDTNTKLDNSNVPYYEATLSTD
                     YYVESKPNASSTDKEFIKAGTRVRVYEKINGWSRINAPQSEQWVEDAYLIDATDM"
     gene            13493..>13766
                     /locus_tag="VBC74_001098"
     CDS             13493..>13766
                     /locus_tag="VBC74_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942054.1"
                     /translation="MIVDLKEIVNDYELNFCGKRCKVETNFKHLPEFMILFDIRDLYH
                     LLGIHKLKTKYRATNWVEAVKADVFLLSNYSKHPNFREVLPRVDNYN"
BASE COUNT         4717 a         2236 c         2807 g         4006 t
ORIGIN      
        1 taaatggaat taacagtagc aaaacgcagt attgaaatta aatttgattt taaaacaatg
       61 tttaagataa ataacagact tggaactgtg aataaagaga caggggaacg taacgctgac
      121 ggcgtcggta cgctatttta taaaatctta gagcgtgatg attcagctgt agtagattta
      181 gtcaagctgt ctgtaggcag cggtaaaaaa gcattgtcag aagatgaagt acttgattct
      241 atcgcagagc tagtcgaaga agaaggatcg acagaagcct tatttaaaga aattgagaat
      301 gacatggttg aatctggttt tttcaaagag aagatttcga aatatatcga aagcatggag
      361 aaatcagtga aatatttaga agctcgagaa gatgcggacg aagctcaaat ccaaatcgta
      421 aaagacatga ttgggaaaat gaaagacgca atctcttaat taactgtgcc caacttggcc
      481 tgacggacat tgatattatt tacaagtgta cgagaacaga attagatgca attatggaag
      541 gattgcagta caagcaaatt gcagaacgtg aaaacctatc tgaacttgct ttaaatctca
      601 gatacacgtt aaatgctaaa aacgtcaatg ctaacaagct caaattagat aaacaacgta
      661 acaaaattaa acgaacttat caaaataaac aagatctaac taacgtttca aatgatttcg
      721 cagaacgtgt ggcgaagctc aatttacatt ttcaaaattt ataacaagaa aggagggatt
      781 ttatggcagg tacatttgat gggtctatat tcgctgatat tggtgcaaat actaaagatt
      841 atgaggcagc tatggctaga attgttagta ctacacaaaa tgcttttaga aaagcgcagg
      901 atactgcagt taacaacagc aacaaaatga tacagatgat tggtcaaatt atggctcaat
      961 tagcaaataa tggtcaatca ttagggcaaa gactcggtaa tgcatatagt acaggtctca
     1021 agttatctct tggtcaaatt caaaggatag cagcatctat cggtgaaaaa attcccgaac
     1081 ccataaagaa tgggttcaat aaagcttttt caattattca aaatagcgta cagaagttat
     1141 cgagcgccat ccctcaaccc ataaaatccg cattcacaag tgcaacaagc gcagtagtta
     1201 gcttttcgtc taagatcaca agtgccgtct cagctgcttt caactctgtt agttctaaag
     1261 cgagtactgt tgcagataaa atcagtagta gttttggggg tagaatcact tcagcggtta
     1321 ctaatttagc gactaagtta agcacaggtc ttagcaatgg ttttagcagg atgtccagtt
     1381 ctgcaactag tgccctaaat ggtattagtc agagattcgc aagcgcatca tctgctggtg
     1441 aaaaactaag aagcactgtg ggaagtatca tttctgcttt tagtttgatg gccattgctc
     1501 ggaaaggaat ccaagcaatc acaggagcaa tggacggtgc aataagtcgt gtagatacca
     1561 tgaaccgctt tccgaaaacg atggcgctat ttgggtattc tgcacaacag tctaaagcat
     1621 caatagataa attatctaaa ggaattgaag gacttcctac accactagac agtgctgtta
     1681 aaagtgccca acaattagct ataactacag gtagcttaga taaaggaaca gagctagcat
     1741 tagcatttaa taatgcaatg attggttatg gagcgactac tgaaggggct gagcaagcac
     1801 ttagacagtt taaccagtcg ttggggtctg gtaagatata tgccgaagag tttaattctg
     1861 tgtctgaagc tgcccctggc ttaatgtcta aaatggcaga gtcattcgga tttggtaaaa
     1921 atggtgttca ggacttaaaa gcagctcttt cagacggcag tattactgcc caagaattcg
     1981 ctgataaaat gattgaacta aacaaggcgc agggtggttt tgctgagatg gcccaatcat
     2041 cggctggtgg aatccgaact gcatggaaga acgtacatac cgccgtcgta aaaggcgtag
     2101 caggtatgat ttcagccttt gatgaagctg ccaaagctaa tggtatgaag accattgcag
     2161 aatccattaa tagtattaag ccagctataa attctgcatt tgcaactgtg aactcaataa
     2221 tgccaaacct tgttgcagca ttcgctagaa tctccaagat ggtgaatatt gactttagtc
     2281 cactaactag tggaattaaa tcagcttttg gattattaaa tactgcttta ggtgaatttg
     2341 caaaaactgg tgaagtatcg tcagcaacta ttgatagaat caaatcaaag atagcatcta
     2401 taggtcctac tgcaatagca gcttgggcct tattaaatcc tgcaagtgct attgcaacta
     2461 ttatgccttt aatatcaatt attggtaaga ttggtgttgc tcttgggact gttggttcag
     2521 cgttgggatc attcggagaa atggtagcca gtggattgtc aactgctagt gggctagttg
     2581 gagcttttgg tagagctatg tcaggacttc ctggaatatt tggtgtagct gcaagtagag
     2641 gtcttgctgt acttgatgga atgacaactg cgattagtag tgctgcaata ctggcaatgt
     2701 ctgctatagg tcctgcagca attttaggat tggtcgtagt aggtcttggt ttagtaaaca
     2761 atcaatttgg tactcaaatt gataaattga taaatacagc aattactaaa ggacctggta
     2821 ttattcaagg ccttgtaaaa ggcattactt ctaaaatacc agctttgata gctagtggta
     2881 ctcaattgat tgctaagttt gctaatgctc taacagtatt attgccagtt attattcagg
     2941 caggtgttca attaattacc agtctggtgc aaggtattgg tcaaaacgca ggtagtttaa
     3001 ttagttcggc tcttaaagtt attggaactt ttgtaagttc aattgctagt gctctccctc
     3061 aaatactagc tgtaggtgtt caattaatac tgaacttagc tcgaggtatt gtacagaata
     3121 tacctttaat aatccagtat gctcaacaaa ttatttctaa ttttggttca agcttacaag
     3181 ctaatatgcc aagcataatt agcaacggta tagcaattag caccaatttg aatgctggta
     3241 ttatccaaat gttgccggca gttatacaaa ttgcaaccca agtcatcaca ggttttattt
     3301 ccggtatagc tcagtatttg ccacaggtaa tccaaggcgg catccagatt ataatcatgt
     3361 tagtgcaagg tattattcaa aatttaccgc aggtaatcca agcagcgatg cagattattc
     3421 agtcattgat atctggttta tcacaagcat taccacaaat tatatctgca gggatacagt
     3481 tagttgtgca gttggctgtt gcaattatta aagggttgcc gcagattatt tcagctggta
     3541 ttcaattgat tgcgggtctt ggtaaagcta tgcttgaggc tattccgaat gctctttcag
     3601 gggtatggga aggcattaaa ggtggattta gttctatgtg ggaccaaatc acaggaaaga
     3661 gctcaacaag tgccgctaag gttacatcag acatgtcaac aatgactatg aatgttggta
     3721 tgcagacgac cacaatggct aatcaaacta acatagacac aactactatg ctaaatagca
     3781 ttagtcaaaa tactggtctt gctaatttaa atgcgacaac aaatgctact cagatggcaa
     3841 caaatgtaaa tgcacaaaca gcatctatgg caaatcaagg tttgctgaat gcggcaggat
     3901 ttaatgaagc gttgagttcg aacatgactt tagcaaatac aaatgctact aatcaagcta
     3961 atcaaatgac tgctaatgtt aataacgcta ctagccaaat gagtctgggt tctctaccga
     4021 atttcttgga attatcgcaa caggcaacaa gtaatatgca aactgcacaa gcaaatgcta
     4081 ccacttccgc gaatggaatg agttctggag ttactggtgc tacagctggt atggctagtg
     4141 gtgtagctaa taacatgtta ggtataacta gcactgtatc tacagggatg aatcaagctg
     4201 caaatgctgc aaaatcaggc ggtgcaaaca tatctaatag tgtgaatagc agttttacac
     4261 aagccaaatc tacagtttca agttcaatgt caggaatgaa cagcactgtt actagtggaa
     4321 tgaatagtat taataatact gtaaaatcag gtggaaataa tattaaatcc acctttacaa
     4381 gttcctttaa ttctgctaag tctacagttt caaatgctat gtctagtatt cgttcgtcaa
     4441 tttcaagtgg aatgaacagc gctgtgagtg taacaagaag tgcaatgaat tcggttgtat
     4501 ctgcaatgaa tgcagcagca agtggtgcta gatctgcagg ttataatact gggattggat
     4561 ttgcaaatgg cctagcaagc actgcaggaa ctattatggc tattgctaat agtattgctg
     4621 ctaatgtcgc tgcaacaatc agaagcgcaa tggatattca ttcgccttcg cgagtaacgc
     4681 gagaacttgg tgcttatact ggtgaaggtt ttgcacttgg tatgcaggat tggatggatg
     4741 atatcaatgc aataggtaaa gagtacgcta atgctgtgac tgatcagcga tgggaaacag
     4801 attcacaatt agcgacatct gcaaacatat caagtgcagg tgtatcatca tcacttgata
     4861 atctaagtga cgaggtacgc aaccaacaat tgactagtcc aacgttcgag gttcataatg
     4921 aacttgttgg tgatgaaatt tatacaacta ttaaatctaa agaagcaaga gaaagtaata
     4981 aagataatta cttcaacttt gcgacataag agaggagaaa aatggattta ttagtacaaa
     5041 agagtgatca atcctttcga ttttcagaga ttggtctacg aatacttgat gtcgatgacc
     5101 gttcatcatc tcttgaggtt gatagtcgaa cagtgaaagg tcgaagcggt aaaatctttg
     5161 ctagtgctaa gtatggcaca aaaaaagtta aggttaatgg gcgcttagtt gtagcttcta
     5221 ttaaagattt tatgacaaaa aaagatgatg tgaatggctt actagtggat agtgagcctt
     5281 tttacattac taaaatgtat cctcagaaag aaaatttata tgatttcgaa ttgcctggga
     5341 tgaaagcagg tgatttagac ttattaaatc aatcttatac agcgtggcac tatcgttgga
     5401 aagtttatgt ctcaagtgaa cctgagtaca cttttgttgg taagtctagt gaaggtctca
     5461 aatacaactt tacaattgca tttgagactg cagaacttcc atttggtgaa actattgcac
     5521 aaaatatagt catcactaac ggaacgttta attattctgg tactgcacct tttagccagt
     5581 tagaataccc atggtctgta gagttaactt caactggtgg acaaagtagt ttttatcttg
     5641 aaatcgatgg gaggcgctgg gcttatcaat atagtgcacc acttaatcaa ggcgacgtta
     5701 ttgtaatcac tggtatatcc acaacattga ataagataaa tgtgactgct agaaccaatc
     5761 atgaatattt tgttttaaag caaaaagtca acaagactat tctctacagc acaaattttc
     5821 aaggcacaat caagttttta aactttaaag aactttacaa gtaaggaagt gatagattga
     5881 caactttttt agatcataaa gataatgagt ttgaagctca agctgttgtt aaaacaacta
     5941 atgcagttaa cggagaaagg tctttatcag gaacaattta tactaacgaa gaggtactaa
     6001 acaaggtcga taaaggttgg aaacttgagt ttgatgatga gtattacaag attatttatg
     6061 ctaaacctac tgatactggt aacaaaattc aagttgaatt tgatgcagtt catcaatttt
     6121 tttatgattt tgataaatca tcactacata agcaattaaa tgatggttca catactttcc
     6181 aagcatattt gaactttatt tttgatggca gcgggtatac ttacagttta gaagtagttg
     6241 tcaaatcttt cgaaaagcaa tcgtttggat ataaatcgcg acttgattta tttaatgaca
     6301 tcattaagag tgcaggagtt gaattttttg ttcgtggcaa ggttgttcga attctacaaa
     6361 aaacaggaac agatttatca acaatcgtac gtaaaaattt caatatgaat gaaatcataa
     6421 ttgaaaagaa aatcaatgat tttattacat atcagaaggg ttttggagcg tgggctgacc
     6481 aagaggatca ttccaaagga aggctcgaag tagaatatga aagtccactt gcaaaggaat
     6541 atggacgaat tgagggtgag cctgttgttg atgagcggta cactaacgct gacaatttaa
     6601 aatcagtact aaaacaaaat gtagattcgt cttacaatat cgctgtaaaa attgacattg
     6661 aagatcttac aaaagcaggt tatcagtaca ctcaacctat tgccggtgat tatattatgg
     6721 ctatcaatga gacactagga tttaaggaaa aaatccgaat agtatcgttt gaaagtgagt
     6781 atgatgtaac aggtcagctg attaaccata aagtcacttg taacgatatt ggtacaatca
     6841 aaaaacaaac tgtagctgtt agccaattat caagtaagat taataataac caagcaatac
     6901 tagataatgc agtatttaaa gcaaatcaag cactagcatc agctgatggt aaaaacacta
     6961 actactatgg tacggagatg ccagtagata atccaaaagg cacgctaaga aagggtgatt
     7021 tgcttttttt aactgttggc gatactacca gaatgtattt ctggaatggt gctgaatgga
     7081 ttatcaattc ttttagcgac gatattgatt ttgtaaagaa agaaattact aataaaatca
     7141 tagaggttaa tgcagcaatg aaacttgctg atgaacagtt aaccgaaaaa tataatgcaa
     7201 ttgttgctaa aaatgttagc caagacgaat tgatcaatca agctaaaact ttatctgact
     7261 ctgcaaaaaa agaggctaca gaagcattaa caaatcttat tagtgaatct aaaaaactga
     7321 ctgaccagat gtctgcgtta tctaaaacag aagttgagca ttattcaacg actaatcagc
     7381 aactgactca aattgatggt acagtcaagg gtttacaaac tagctatgaa gcattgttaa
     7441 aaaaagatgg agatatcact caggcacttg ctaattataa gcaaacaatt gaccaaaaca
     7501 gtgcaagtat tatgaccaac aaaaagactg ccgataatgc acttagcagt ctacagacac
     7561 aagtcataca aacgtcgaac gaaattacaa cgagattatc tcaaacaaat attgaagcgt
     7621 tgattacaag caagtctgcc gttatcgctg ataataaagt caaggaaact gcagatagtt
     7681 ttagtagaga attaactcgt gttaataata ctattgataa tttaaagata ggtacagtta
     7741 atctactatc tggtactaaa ggttggacta aaaattggtt taatcgtaac aactggcaag
     7801 ctgatgatga cagcttgtct attggtaatt tcaacttaac agtgctaaag cgtaagatga
     7861 tgtggagtgg tatatcacaa tcatatgctg taaaaagcgg agagagttat gtttttagtg
     7921 catatgctaa atcaagcata gataatgatg ttgttaattt atatttgtta ccaaatgatc
     7981 aatcgcaaca ggctaagata agtattagtt tcaagtcgat cactttaaac acggattggc
     8041 aacgtatcgt tattaaattt aacgtaaatt ctgatggcta tattttacca cgcttcgaac
     8101 gctttaatga aaatggttgg ttgtatattg caggttacca gcttgaacat ggaaatgtta
     8161 caagtgacta ttcagagtcc ccagcggatc aagaagataa aatgactgtt gaatttaata
     8221 aaatcaacga tacagttaat agtcatagtc aactgattgg taaacaaaac gagagtctga
     8281 cagctacaat acaaaaagtt gataatatac aatcaacagt atctaatgtg gatggacgct
     8341 tgtcacgagt tacccaaacg gctgacggta tagttgcaac agttagtcag cttgataaca
     8401 acattttacc tggcacaaaa aaattcacag gatggcaaac agaaggtgca gtactagagg
     8461 aactaacaca atcgccttac ccatttgttt ttaaaaaatg ggtaagcggt aataaggtgt
     8521 cacctttaat cgaatatgat gtcaaaaaag atcaagagta tacgtttact gcatacattt
     8581 ctagagaaca agcaggaaat ctatatttct acttgtatga tctttggaat catcatatta
     8641 caagcacaac tcctcgtgaa actattatta aagacgtcac tcaaaccatt agacgattta
     8701 aaataacatt tattccaact cgagacggta aaattagacc acgctttgcc atgcttgcta
     8761 gtgatccagg tagctttagt accggtggtt ttatgttatc taaaggtaca gttgagttgg
     8821 aatggtctga atctaagcaa gatattaaag atagtgtgac tgctattaat acaacagtta
     8881 cacaaacggc taacggttgg gctattaaaa atttaacttc tagcggtact gtgttgagtc
     8941 agcttaattt gacggacggt aatgtaaaac tggaaggtaa gttaatacac ttgtcaggac
     9001 agtctattat cgacaatggc attattacta atgcgatgat taaagattta actgcagata
     9061 agattacagc aggtacaatt gatgctaatc gtataaatgt cattaattta gatgctagca
     9121 acatcactag tggcaggatg tcagcagatc tgatacgtag cggcgtttta atatcgcaaa
     9181 acggtgcaat gttagcagat cttaatacag gacaaattga attttacaca gatagtccag
     9241 ctatcaaacg tattactgca ggttatccta accaattcgt taaatttgca acaggtaatg
     9301 ttgaaggaaa gggcgcggct ggcgtaacgg ttataggttc taatcgatgg ggaagtgaat
     9361 cgtcaaacga cggtggtttc gtaggtattc gcgcttggaa tggtaaagac atcgatactc
     9421 ttgatttagt tggcgatagt attcacttag caagctctgc gtacactaac gctgatggtt
     9481 gggaaatcat tactttgcca aatcagctat caatagatgc acgtaacata aatcatcgtg
     9541 taacgtctaa gataaaagta ggagatattt ggctatggaa aaatacatca acttatacaa
     9601 gcatgaaaga tacaattaat atgattattg ataacttgca gctactacac aataataaaa
     9661 ccactgaaaa aggctacagt tatactattc caggaaagat ttaggagaaa aaatggaaga
     9721 aaaaataaaa tcaaatttag ctttacaaat agcgcaatta tctttagata aagcaactct
     9781 acaagctcaa aatgagcaat tacaattaca aattgctgag ttaaatcagc aattagatga
     9841 gataactaag ccaggagcaa agtgaggtga gtaacatgaa acaatggaac attgttggaa
     9901 aaccatttcc aatctatcaa gatggagtgg ttctaaaaac ggatgtacgc ttacaaggcg
     9961 acgatggtac atatttacca caagagctaa ttggtaatca tgttgataag gataatcaat
    10021 cactgataaa acttgtactt gaaacttttg caaaagaaaa tgtcgttgat ttcgcaatgc
    10081 ttgagtctgt aaaagatatt gagcaactaa aagctgacaa agtaacttta atcaaaaaaa
    10141 ttgcagaggc tgatgaggca atttctaaga tgtcgcaagc aacaaaagat atgcagctta
    10201 aagttgataa agcagttgtc gagttaactg acttagtgac atcaagtgta tctagccttt
    10261 ccgtgccgtc tacatcgatg gaatcaagct aatggaacta acgttgaata ccgttataac
    10321 actatattat ttaataaaaa aaggagaatt taatatgaca tatactttta aaaaagatca
    10381 tcttattgtt gacgtttggt accgtcgtgt cgcgaatggt atttgtgatt tcgaagcagt
    10441 acctaaatta tttaatttac gagatgttgt gcttgagtta cttagtcaaa aagttactga
    10501 taaagaatca gagtgaggta acttaatgat agattttgtg caagttggcg cttttgcagg
    10561 tgctgcttta tctattttgg gagtatgggg atttattgtt aatcccttta aaaaagcaat
    10621 ggaagctaac gagattgcta tggctcagct caaggactca ctcaaagaac tagcatacga
    10681 gctcaaaaat atagatagag accgagagat tacaaaagct gttattgatc gtcacgagga
    10741 gcgtttaggt cgtgtcgagg atgatatcat catcaataaa gagcaaatca aaacattatt
    10801 taataaggag aaataagatg attaattgga aagtaagaat taaaaacaaa gcattttggt
    10861 cagcaattat tccagcaata tttttagttg tacaagcagt tgcaaatgtt tttggttata
    10921 cacttgaact tagtgattta ggtaataaat tgttagtagt tgttaatagt gggttttcag
    10981 tacttgttat tgcaggtatt gttacagacc ctactacaca aggagtatca gatagcagcc
    11041 gtgcgctatc ctacacagag ccaaaataat aggaggataa aatgaaagca atcactaaaa
    11101 tagcaatggt actagcaata gcaatactgt acataccgct tgcagtggtt gcttttttta
    11161 gttatccgat ttatttactt tttggaaagg aggagtaaat ggcaacttat caagaatata
    11221 aaagcaggtc aaatggtaac gcttatgata ttgatggttc gttcggtgca caatgctggg
    11281 acggttatgc agattactgt ggatttttag gtgtaccgta cgcaaactgc acaaatacag
    11341 gatacgcaag agacatctgg gaacagcgcc acgaaaatgg tatcttaaac tactttggcg
    11401 aagtagagac tatgcaagcg ggagatgtcg cgatttttat ggttgttgac ggtgtaacac
    11461 cttacagtca tgtagcaatt tttgacagcg atgcgggtgg aggctatggt tggtttttag
    11521 gacaaaacca aggtggacca aacggagcat ataatttagt aaaaatacca tattcagcga
    11581 cttatcctac tgcatttaga ccaaaagttt ttaaaaatgc agttactgtt acaggtaata
    11641 ctggcttaaa taaaggtgat tattttatcg atgtatcagc ttatcagcaa gcagatttaa
    11701 caacgacttg tcagcaggca ggtactacaa aaacgattat caaggtatcg gagtcaatag
    11761 cgtggctatc tgacagacat caacaacaag caaacacaag cgacccaatt ggctattacc
    11821 actttggacg ttttggcggt gatagtgctt tagcgcaacg agaagcagac ttatttttgt
    11881 ctaacttacc aagcaaaaaa gtatcatact tagtcattga ctatgaagat tccgcaagcg
    11941 cagacaagca agctaacaca aatgcagtta ttgcatttat ggataaaatc gctagcgctg
    12001 gttacaagcc tgtttactac agctataagc catttacact taataatgtt gattatcagc
    12061 agattattgc taaataccca aatagtattt ggattgcagg ctatcccgat tacgaggtgc
    12121 gctctgaacc actgtgggac tattttccat ctatggacgg cgtgcgctgg tggcaattta
    12181 caagtgtagg agtagcaggt ggtttagata aaaatattgt attattagca gatgatagta
    12241 gcaaagtgga tatacctaag attgacaaac cacaagaacc acaaagccag cttactttta
    12301 atcaaaagct agatactaac actaaattag acaactcgaa tgtaccttac tacgaagcga
    12361 ctctaagcac agattattac gtagaatcta aaccaaacgc aagtagtaca gataaagagt
    12421 ttatcaaagc aggtactcgt gtgagagttt acgaaaaaat caacggttgg tcacgtatta
    12481 acgcaccaca atccgaacaa tgggtagaag atgcttactt aattgatgca acagatatgt
    12541 aaaccaacag agcgacataa atgtcggtct gttaatggtg taagttacac cgcaactaaa
    12601 aaaccagttt aggaggtatt attttattgt taataataaa tattactgtt tttcttgact
    12661 tagcaaataa aaacaggtaa tataaagata caaataacga caaaaaactg cgcctctgtt
    12721 gttttgtgct cttgttgttt gtattgctgt acacctatcg ccgtaggtga cgaacaaaaa
    12781 atgtaagagg ggactccaac ctcagaaaag cacagtttgc cggctgtgct tttatttttt
    12841 gaacataaaa aagagcgggt ttgccgaccc actcttaaac aaaaaatgta atgtacgtac
    12901 tattttttgt gtaaatagtc acgtacgata tcagctaagc aattcgcagt gagagtaacc
    12961 acaaaattgc taactaatgt gaggaggaaa aattccatac tccaacctcc tttttaaaga
    13021 tttgctattt gattagttag ctctagccca tctaagctac tagagcttat ttgttgattt
    13081 tattatatct gattttgcaa ttttaaaaaa ggtcttagct gctaaaaaaa acaagaactt
    13141 ctagtattat aagacaaaac cgctcagatt aacttctggg ctgtttttta ttgcaaaaaa
    13201 tttcgcattt attgacaaaa agttcaagac atgtcataat gagggtggtt atagaagata
    13261 aatttcgttc atttatcatc atttctaacc caacgtcttc gttgttcgtt gaacccgtag
    13321 tgatacgggc gtatgactga aagcacatca tacggcttgg cagagcttaa gaactgttct
    13381 cttgcgataa gcctaagaag cacaatagag agttagagtt ttgcacctct aatcgttagc
    13441 cctgaccgga ggatatttcc ggtccgtgct tttttatttt gtagaaagtt ttttgatagt
    13501 ggatctcaaa gaaatagtta atgattatga gctaaatttt tgtgggaaaa ggtgtaaagt
    13561 tgagactaat tttaagcatt tacccgaatt tatgatttta tttgatataa gagatttgta
    13621 tcatcttcta ggtattcata agttgaaaac aaagtatcgc gcaacaaatt gggttgaagc
    13681 tgtgaaagca gatgttttcc tcttatcgaa ttattcaaaa catccaaatt ttagagaagt
    13741 tcttcctaga gtcgataatt ataatt
//
LOCUS       DATROD010000039        13562 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.039, whole genome shotgun sequence.
ACCESSION   DATROD010000039 DATROD010000000
VERSION     DATROD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13562
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.039"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            79..513
                     /locus_tag="VBC74_001099"
     CDS             79..513
                     /locus_tag="VBC74_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6942055.1"
                     /translation="MEYDQINSYLVDIFNRIMIIEEMSLKTSQFSDVSLKEMHTIEII
                     GKHSEVTPSDVARELMLTLGTVTTSLNKLEKKGYIERKRSSIDRRVVHLSLTKRGRLL
                     DRLHSKFHKSMVSHIIEDLGEEDIKMLTSALGNLHKFLEDLV"
     gene            513..1484
                     /locus_tag="VBC74_001100"
     CDS             513..1484
                     /locus_tag="VBC74_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEO6942056.1"
                     /translation="MVFAKISQLAHYAPSQIIKNEDLSLIMDTSDDWISSRTGIKQRH
                     ISKNETTADLANKVAEQLIEKSGYSASQIDFIIVATMTPDSMMPSTAARVQAHIGASN
                     AFAFDLSAACSGFVFALSTAEKLISSGSYQKGLVIGAETVSKVLDWTDRGTAVLFGDG
                     AGGVLLEASKEKHFLAESLNTDGSRQGLQSSQVGLNSPFSDEVLDDKFLKMDGRAIFD
                     FAIKEVSKSINHLIETSHLEKEDIDYLFLHQANRRILDKMSRKIDIARDKFPENMMDY
                     GNTSAASIPILLSESYENGLLKLDGNQTILLSGFGGGLTWGSLIVKI"
     gene            1542..1766
                     /locus_tag="VBC74_001101"
     CDS             1542..1766
                     /locus_tag="VBC74_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEO6942057.1"
                     /translation="MAVFEKVQEIIVEELGKDAEEVTLNTTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKVK"
     gene            1921..2880
                     /gene="fabK"
                     /locus_tag="VBC74_001102"
     CDS             1921..2880
                     /gene="fabK"
                     /locus_tag="VBC74_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEO6942058.1"
                     /translation="MKTRITELLNIKYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSMTDKPFGVNIMLLSPFVDDIVDLVIEEGVKVVTTGAGNPGKY
                     MERFHEAGITVIPVVPSVALAKRMEKLGADAIITEGMEAGGHIGKLTTMTLVRQVVDA
                     VTIPVIAAGGIADGRGAAAGFMLGADAVQVGTRFVVAKESNAHPNYKAKILKAKDIDT
                     AVSAQVVGHPVRALKNKLVTTYSQAEKDYLAGRISINEIEELGAGALRNAVVDGDVIN
                     GSVMAGQIAGLIKSEETCQEILEDIYSGARQVILSEASRWSDL"
     gene            2900..3826
                     /gene="fabD"
                     /locus_tag="VBC74_001103"
     CDS             2900..3826
                     /gene="fabD"
                     /locus_tag="VBC74_001103"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEO6942059.1"
                     /translation="MNKVAFLFAGQGAQKLGMARDLYETFPIVKETFDKASHVLGYDL
                     RELIDKDLDKLNQTKYTQPAILTTSTAIYRLILKEIGLRPDMVAGLSLGEYSALVASG
                     AIRFEDAVVLVSRRGQLMEAAAPAGSGKMVAVLNADRQIIEDACKKASQFGIVSPANY
                     NTPKQIVIGGESIAVNAAVEELKQQGVKRLIPLNVSGPFHTALLKPASQKLSDVLDKV
                     HFSVSEIPVIGNTEAQIMKKDDIKSLLARQVMEPVRFDESIETMKKMGMTQVVEIGPG
                     KVLSGFLKKIDSSLSVYSVEDKIGFNNLKELN"
     gene            3835..4569
                     /gene="fabG"
                     /locus_tag="VBC74_001104"
     CDS             3835..4569
                     /gene="fabG"
                     /locus_tag="VBC74_001104"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEO6942060.1"
                     /translation="MQLKDKNIFITGSSRGIGLAIAHQFAQLGANIVLNGRSEISEDL
                     IAEFADYGVKVIAISGDVSSFEDANRMIKEAIASLGSVDVLVNNAGITNDKLMLKMTV
                     EDFESVLKINLTGAFNMTQSVLKPMTKARQGAIINISSVVGLTGNVGQANYAASKAGL
                     IGFTKSVAREVAARGIRVNAIAPGFIESDMTDVIPEKMQEAILAQIPMKRIGKGKEVA
                     QVASFLAEQEYLTGQVIAIDGGMTMQ"
     gene            4585..5817
                     /gene="fabF"
                     /locus_tag="VBC74_001105"
     CDS             4585..5817
                     /gene="fabF"
                     /locus_tag="VBC74_001105"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEO6942061.1"
                     /translation="MTLQRVVVTGYGVTSPIGNTPEEFWNSLKEGNVGIGPITKFDSS
                     DFMVKNAAEIHDFPFDKYFVKKDLNRFDMYSLYALYASSEAIQHANLNLDEIDADRFG
                     VIVASGIGGIQEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMRLGAHGVCKSI
                     NTACASSNDAIGDAFRNIKFGIQDIMVVGGAEAAITKFAIAGFQSLTALSTTEDPSRA
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     LGATKAIQLALAEANIKPEEVNYVNAHGTSTPANEKGESQAVVAALGTDVPVSSTKSF
                     TGHLLGAAGAVEAIATIEAIRHSYVPMTAGTTELSEDITANVIFGQGQDADIRYAISN
                     TFGFGGHNAVLAFKRWED"
     gene            5783..6319
                     /locus_tag="VBC74_001106"
     CDS             5783..6319
                     /locus_tag="VBC74_001106"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEO6942062.1"
                     /translation="MQFWPSNAGRIKLDIQEIKDLMTQFDESSLREFSFKTSDGELSF
                     SKNEGKAPLVPTMSPMSHQPEATPTIATPVSNEAGEQTKQATEVVSEIPESTVTVAEG
                     DVVESPLVGVAYLASGPDKPNFVSVGDSVKKGQTLMIIEAMKVMNEVPAPHDGVVTEI
                     LVANEEVIEFGKGLVRIK"
     gene            6316..6738
                     /gene="fabZ"
                     /locus_tag="VBC74_001107"
     CDS             6316..6738
                     /gene="fabZ"
                     /locus_tag="VBC74_001107"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEO6942063.1"
                     /translation="MIDIKEIREALPHRYPMLLVDRVLEVSEDEIVAIKNVSINEPFF
                     NGHFPEYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTAKFVKRRGTIAVVEAIAEVDGKLAASGTLTFAIGN"
     gene            6776..8146
                     /gene="accC"
                     /locus_tag="VBC74_001108"
     CDS             6776..8146
                     /gene="accC"
                     /locus_tag="VBC74_001108"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEO6942064.1"
                     /translation="MFKKILIANRGEIAVRIIRAAREMGISTVAIYSEADKESLHTIL
                     ADEAICVGPAKSAESYLNVNAILSAAIVTGAEAVHPGFGFLSENSKFATMCEEMNLKF
                     IGPSGEVMDKMGDKINARAEMIKADVPVIPGSDGQVTSVEEAVSIAEEIGYPLMLKAS
                     AGGGGKGIRKVKSADELKPAFESASQEALAAFGNGAMYIEKVIYPARHIEVQILGDSF
                     GKIVHLGERDCSLQRNNQKVLEESPSVAIGNTLRQQIGEAAVRAAEAVSYENAGTIEF
                     LLDENSGQFYFMEMNTRVQVEHPVTEFVTGVDIVKEQIRIAAGIPLSVSQNDIKLTGH
                     AIECRINAENPQFNFAPCPGTINGLHLPAGGMGLRVDSAVYTGYTIPPYYDSMIAKVI
                     VHGENRFDALMKMQRALYELEIDGIITNTEFQMDLISDKKVLAGDYDTSFLMEDFLPR
                     YYGNNQ"
     gene            8155..9030
                     /locus_tag="VBC74_001109"
     CDS             8155..9030
                     /locus_tag="VBC74_001109"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEO6942065.1"
                     /translation="MALFSKKDKYIRISPNKALGSSDKRSLPEVPDELFAKCPSCKHM
                     IYQKDLGLAKICPACSYNFRISAQERLLLTVDEDSFEELFTGIETKDPLNFPNYREKL
                     AATRQKTNLDEAVVTGLAKIKGQTTALAIMDSHFIMASMGTVVGEKLTRLFELATEKK
                     LPIVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNQGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQALVGFAGRRVIETTVREDLPEGFQKAEFLLEHGFVDAIINRTELRDC
                     IAQLIAFHGNEVGHE"
     gene            9023..9796
                     /locus_tag="VBC74_001110"
     CDS             9023..9796
                     /locus_tag="VBC74_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEO6942066.1"
                     /translation="MNNDVTRILKDARDQGRLTALDYAELIFDNFMELHGDRQFADDK
                     SIIGGLGYLAGRPVTIVGIQKGKNLQDNLDRHFGQPHPEGYRKALRLMKQAEKFGRPV
                     ITFINTAGAYPGVGAEERGQGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALAVAD
                     KVWMLEHTVYSILSPEGFASILWKDGTRTTEAAQLMKMTAGELYHMEVVDKVIPEHGY
                     FSSEIVDMIKTSLISELEMLSQLSLEDLLEQRYQRFRKY"
     gene            10264..10896
                     /locus_tag="VBC74_001111"
     CDS             10264..10896
                     /locus_tag="VBC74_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEO6942067.1"
                     /translation="MYQELNDYLEFQSMKYIMPEKAGPLKDDMQTFKAKGQLARNSFT
                     ELSRALEQRMDGFKMQRVSNWANQAQVGRPHFWVYYRKDTDQLDDVAVALRVYGVKDS
                     FGVSLEVSFVERQKSDKTLEKQARVLSIPIASPLYFMVQRQGETHREEGNEENRQRLM
                     QEIKSGKVRKVLVKYDVLLTENQSLENILQRLLEGFEKVLPYYQVCQTIS"
     gene            complement(10942..12219)
                     /gene="serS"
                     /locus_tag="VBC74_001112"
     CDS             complement(10942..12219)
                     /gene="serS"
                     /locus_tag="VBC74_001112"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEO6942068.1"
                     /translation="MLDLKRIRTDFDAVAKKLATRGVDQETLTTLKELDIKRRELLIK
                     SEEAKAQRNVASAAIAQAKRNKENADEQITAMQTLSADIKAIDAELADVDANLQSMVT
                     VLPNTPADDVPLGADEDENVEVRRWGTPREFDFEAKAHWDLGESLGILDWERGAKVTG
                     SRFLFYKGLGARLERAIYSFMLDEHAKEGYTEVIPPYMVNHDSMFGTGQYPKFKEDTF
                     ELADSPFVLIPTAEVPLTNYYRDEIIDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLNLPYRVITLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEVDGKVRLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRPYMGNIDIIKPN"
     gene            12518..>13562
                     /locus_tag="VBC74_001113"
     CDS             12518..>13562
                     /locus_tag="VBC74_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEO6942069.1"
                     /translation="MEKYTSIKQSGRHYGLDLLRIISMFMIVITHVLGKGGLRSSVEG
                     HADSYFIVTWIIQVLVYGAVNCYALISGYVGINSRYRYSKLLSIWTQVFFYTFTITAL
                     FAITGHEVTLLNWRDAFFPIVSGQYWYITAYFGLLVFMPVINNGLNALTDKQLKQLVL
                     LMFIIFSILPAVLNNRVPEFSLSKGFEMTWLLILYIIGAYLKRIDLNIFKTSYLLIVY
                     LLSLVATYVMKFSIGDIWYWYVSPTLTLGAVSLFILFARASIKPSGFLKKIIVVLAPS
                     TLGVYLCHLHPLIVKYFVRDFAETFVYESIYLYPFLILGAGILIYLLAFIVERIRLRI
                     FKAIHFSNFLRWFD"
BASE COUNT         4286 a         2085 c         2913 g         4278 t
ORIGIN      
        1 ttaagattaa agtcttgcaa ataagtttga actttgatat aatttgacta tcaaagttta
       61 atgtgaggtg gtgatatctt ggaatacgat cagattaata gttatttagt tgacattttt
      121 aatagaatca tgattattga ggaaatgagt ttaaagacta gccaatttag cgatgtatct
      181 ttaaaagaaa tgcatactat tgaaattatt gggaaacatt ctgaggtaac tcctagcgat
      241 gtcgctcgag aattaatgtt aactttggga actgtaacaa ccagccttaa taagttagaa
      301 aaaaaaggct acattgagcg taaacggtca agtattgatc gtcgagttgt tcacttgagt
      361 cttactaaga gaggtaggtt attagatcga ttgcatagta aatttcataa atctatggta
      421 tctcatatta ttgaagacct aggcgaagaa gacataaaaa tgttgacgag cgctttaggg
      481 aatcttcata aatttttgga ggacttagtc tagtggtttt tgctaaaatt agtcaacttg
      541 cacattatgc accttctcaa atcattaaaa atgaggattt atcattaatc atggatacca
      601 gtgatgactg gatttcgtca cgtactggta taaaacaacg tcatattagt aaaaatgaaa
      661 caacagctga tttggcaaat aaggtcgcag aacaacttat tgaaaaatcc ggttattccg
      721 ctagtcaaat tgactttatt attgttgcaa cgatgacacc agactctatg atgccttcta
      781 ctgccgcaag agtgcaagct catattggag cgagtaatgc ttttgccttt gacttgagtg
      841 cagcatgtag tggttttgtt tttgccttgt ctaccgccga aaaattgata tcatcaggta
      901 gctatcaaaa aggtttagta ataggagcag aaactgtttc taaagtatta gactggactg
      961 atagagggac agcagtttta tttggagatg gagctggagg tgtcttgcta gaagcttcta
     1021 aggaaaaaca ttttcttgca gagtctctca atacagatgg tagtcgtcag ggacttcaat
     1081 ctagtcaagt cgggttaaat tcacctttct cagatgaagt acttgatgat aaatttttga
     1141 aaatggatgg tagagcaatc tttgattttg ctatcaaaga agtttcaaaa agtatcaatc
     1201 atcttataga aacatcccat ttggaaaaag aagatattga ttatcttttc ttacaccaag
     1261 cgaatcgaag gattttagat aagatgtctc gaaaaattga tatagcacgt gataaatttc
     1321 cagagaatat gatggattat ggtaatacta gtgcagctag tatccctatt ttactttcag
     1381 aatcttatga gaacggtttg ttgaaactag atggtaacca aacaatcctc ttatcgggtt
     1441 tcggtggagg tttgacatgg ggaagtctaa ttgttaaaat ttagttatat atagtgcata
     1501 gcacatttat aaaagattat ttaaaattta aggagctaaa aatggcagta tttgaaaaag
     1561 tacaagaaat tattgttgaa gaacttggta aagatgcaga agaagtaacg ttgaacacta
     1621 cttttgacga tcttgatgct gattcgcttg atgttttcca agtgatttca gaaattgaag
     1681 atgcatttga tatccaaatt gagacagaag aaggactaaa tactgttggt gatttagttg
     1741 cttatgttga agaaaaagta aaataattaa gtcgtaagaa aaaaagaagc aagtgataat
     1801 aaaagatatt cttaccaaat cacttattag agtgagtatt caatcaatcg ttgatcggta
     1861 ctcactcttc ttcttatcaa tatagtttga ttgtcaaatt attaatttta ggaaagaaaa
     1921 atgaaaacac gtataacaga attactaaat ataaaatatc caatttttca agggggtatg
     1981 gcgtgggtcg ctgatggaga tcttgcagga gctgtttcta aggcaggtgg tctaggtatt
     2041 attggtggag gaaatgcccc taaagaggtt gtcaaagcaa atattgataa aataaaatct
     2101 atgactgata aaccctttgg tgtgaatatt atgcttttat caccttttgt agatgatatt
     2161 gttgatttgg ttattgaaga aggtgtaaaa gttgtaacga ctggtgcagg taatcctggt
     2221 aagtacatgg aacgctttca tgaagcaggt ataacagtaa tccctgttgt tcctagtgtt
     2281 gcattagcaa aacgtatgga gaaattaggg gctgatgcta tcattacaga aggaatggaa
     2341 gcgggtggtc acatcgggaa gctgaccacg atgacattag tgcgacaggt tgttgatgct
     2401 gttacaattc cagtcattgc cgcaggtggt atagcagatg ggcgtggggc tgctgcgggt
     2461 ttcatgttag gagctgatgc tgttcaggta ggaactcgtt ttgttgttgc taaagaatct
     2521 aatgctcacc ctaattacaa agcaaaaatt ttaaaagcaa aagatatcga cactgctgtt
     2581 tctgctcagg tagtaggtca tccagttcgt gccttaaaaa ataaactagt aacaacctat
     2641 agccaagcag aaaaagatta tttagctggc cgtatttcaa tcaatgagat tgaagaatta
     2701 ggagcgggag ctcttcgcaa tgcagttgtt gatggggatg ttattaatgg ttcagttatg
     2761 gcaggacaaa ttgcaggatt aattaaatca gaagagactt gtcaagagat tcttgaagat
     2821 atttattcag gagcgcgtca agttattctt tctgaggcaa gccgctggtc agatttataa
     2881 atacaaataa ggagacacta tgaataaagt tgcatttcta tttgctggtc aaggtgctca
     2941 aaagttagga atggctagag atttatatga aactttcccg attgttaaag aaacttttga
     3001 taaggcaagt catgttttag gatatgattt gagagaattg attgataaag acttagacaa
     3061 attaaatcag acgaaatata cgcaaccagc tattttaacg acttcgactg ctatttatcg
     3121 tttaattctt aaagaaattg gacttagacc agatatggtt gcaggacttt ctttgggaga
     3181 atatagtgct ttagttgctt caggtgctat aagatttgaa gatgctgtag tacttgtttc
     3241 tagacgaggc caactaatgg aagcagcagc acctgctggt agtggcaaaa tggtagcggt
     3301 tttaaatgct gatcgtcaaa taattgaaga tgcttgtaag aaggcttctc agtttggtat
     3361 tgttagtcct gcaaattata atactccaaa gcaaattgtt atagggggag agtcaatcgc
     3421 tgtaaatgca gctgtagaag aactaaaaca acaaggagta aaacgtttaa ttcctctgaa
     3481 tgtttcaggt ccttttcaca ctgctttatt gaaacctgca agtcagaaat tgagcgatgt
     3541 tttagataaa gttcattttt cagtttctga aatccctgta attggaaata cagaggcgca
     3601 gataatgaaa aaagacgata tcaaatcttt attggcacgc caagttatgg aacctgtacg
     3661 ctttgatgag tctattgaaa ccatgaaaaa aatgggaatg acacaggttg ttgaaattgg
     3721 tccagggaag gtactctcag gtttcttgaa aaaaatagat agctcattat cagtatattc
     3781 tgttgaagat aaaattggat ttaataatct taaggaatta aactagggga aaatatgcaa
     3841 ttaaaagata agaacatttt tattacgggt tcaagtagag gaatcggcct tgcaattgcc
     3901 catcaatttg cacaattagg tgctaatatt gttttaaatg gacgttctga aatatcagaa
     3961 gacttaatag ctgaatttgc tgattatggt gtaaaagtta ttgctatttc aggagatgtt
     4021 tcttcttttg aagatgctaa tcgcatgatt aaagaagcta ttgcaagtct aggtagcgtg
     4081 gatgtgcttg ttaacaacgc tggtattact aatgataaat tgatgcttaa aatgactgtt
     4141 gaagattttg agagcgttct taaaattaat ttaacaggtg catttaatat gacacaatca
     4201 gtcttgaaac cgatgacaaa agcgcgtcaa ggtgctatta ttaatatctc tagcgtcgtt
     4261 ggattaactg gaaatgtagg gcaagctaat tacgcagcct ctaaagcagg tttaattggc
     4321 tttacaaaat cagttgccag agaagtagca gcacgaggca ttcgtgtcaa tgccattgct
     4381 ccaggcttta tcgaatctga tatgacagat gttatcccag aaaaaatgca agaagctatt
     4441 ttagctcaaa ttccaatgaa acgtattgga aaaggtaaag aagtagcgca ggtagcgtct
     4501 ttcttagcag aacaagaata ccttacagga caagttattg ccattgatgg cggtatgacg
     4561 atgcaataat aaagaggaaa taatatgaca ttacagcgag tagtagtaac aggttacggt
     4621 gtaacctcac ctattggaaa cacaccagaa gaattttgga atagccttaa agaaggaaat
     4681 gttggtattg gacctatcac taaatttgat tcaagtgatt tcatggtcaa aaatgctgct
     4741 gaaattcatg attttccatt tgataaatat tttgtcaaaa aagatctgaa cagatttgat
     4801 atgtattccc tttatgcctt atatgcttca agtgaagcta ttcaacatgc taatttaaat
     4861 ttagatgaaa ttgatgcaga tcgatttggt gtcatcgttg catcaggtat tggagggatt
     4921 caagagattg aagaacaagt gattcgtctt catgaaaaag ggccaaaacg cgtaaaacca
     4981 atgacgcttc caaaagctct accaaatatg gcagctggaa atgttgcgat gaggttaggc
     5041 gctcatggtg tatgtaaatc aattaataca gcttgtgctt cttctaatga tgctattgga
     5101 gatgctttcc gtaatataaa atttggtatt caagatatta tggtagtcgg tggtgcagaa
     5161 gctgctatta caaaatttgc cattgctgga ttccaaagtc ttacagcatt atcaacaact
     5221 gaagatccat caagagcatc tattccattt gataaagatc gcaatggatt tatcatggga
     5281 gaaggttctg gaatgttggt cttagaaagt ttagaacacg ctgaaaaacg aggagctact
     5341 attcttgcag aagttgttgg ttatggtaac acttgtgacg catatcatat gacttcacca
     5401 catccagaag gtttaggagc cactaaagct attcaacttg cactggctga agctaatatt
     5461 aaacctgaag aagttaacta tgtaaatgct catggaactt caacacctgc taacgaaaaa
     5521 ggagagagtc aagctgttgt tgctgcactt gggacagacg ttccagtttc ttcgactaaa
     5581 tcttttactg gtcatctttt gggtgccgca ggagctgttg aggcaattgc aactattgaa
     5641 gcgatacgtc atagttatgt tccaatgact gctggaacaa cagaattatc agaagatatt
     5701 acagcgaatg ttatttttgg tcaagggcaa gacgctgata ttcgttatgc catttcaaat
     5761 acatttggtt ttggaggaca caatgcagtt ctggccttca aacgctggga ggattaaatt
     5821 ggatattcaa gagattaagg atttaatgac tcaatttgat gagtcaagtc tgagagaatt
     5881 ttcattcaaa acaagtgacg gtgagctgtc ttttagtaaa aatgagggaa aagctccact
     5941 tgtaccaact atgtctccta tgagtcatca acctgaagcg actccaacta tagcaactcc
     6001 tgtatctaat gaagcaggtg agcaaacgaa acaagctact gaagtagttt cagaaatccc
     6061 agagtcgact gtgactgttg cagaaggtga cgttgtcgaa agtcctttag ttggggttgc
     6121 ttatttagct tctggaccag ataaacctaa ctttgtttca gtaggtgata gcgtgaagaa
     6181 gggacaaact ttgatgatta ttgaggcaat gaaggtgatg aatgaagtgc ctgcccctca
     6241 tgatggtgta gttactgaaa ttcttgtagc caatgaagaa gttattgaat ttggtaaagg
     6301 attggtacga atcaaatgat tgatataaaa gaaattcgtg aggctttacc tcaccgctat
     6361 ccaatgttat tagtggatcg tgttttagag gtatcagaag atgagatagt cgctatcaaa
     6421 aatgttagta tcaatgagcc ttttttcaat ggtcattttc cagaatatcc tgtaatgcca
     6481 ggcgttttaa ttatggaagc tttggctcaa actgcaggtg ttttagaatt atctaaagaa
     6541 gaaaacaaag gaaagcttgt tttttatgca ggtatggata aagtgaaatt taaaaagcaa
     6601 gttgttccag gagatcaatt agttatgact gctaagtttg tcaaacgtag aggaactata
     6661 gccgttgttg aagcaatagc agaagttgat gggaaattag ctgccagtgg aacgttaaca
     6721 tttgcaatag gtaattgaag atagtattga atttagaggg agagaagcat caaaaatgtt
     6781 taagaaaata ttgattgcta atcgcggaga aattgcagta cgtatcattc gtgcagcccg
     6841 tgaaatggga atttcgacag ttgctatcta ttcggaggct gacaaggaat cattgcacac
     6901 aattttagca gatgaagcta tttgtgttgg accagccaaa tcagcagaat cttatttaaa
     6961 cgttaatgct atattatctg ctgctattgt gactggagcg gaagctgttc atccaggttt
     7021 tgggtttttg agtgaaaatt ctaaatttgc tactatgtgt gaagagatga acttaaaatt
     7081 cattggtcca tctggagaag ttatggataa aatgggtgat aaaatcaatg ctcgtgcaga
     7141 gatgataaaa gctgatgttc cggttatccc aggatcagat ggtcaagtta caagtgtaga
     7201 agaagctgta tcaattgctg aggaaatcgg ctatccattg atgttaaaag cctcagccgg
     7261 tggcggtgga aaaggaatta ggaaggtcaa atcagcagat gaattgaagc ctgcttttga
     7321 gtctgcttca caggaggctt tggctgcgtt cggtaatggt gctatgtata ttgaaaaagt
     7381 tatataccca gctagacata ttgaagtaca aatacttgga gatagctttg gtaagatagt
     7441 tcacctagga gaacgtgatt gttctctgca gcgtaacaat cagaaggtat tggaagaaag
     7501 tccgtctgtg gcaataggta acactctgcg tcaacaaatt ggtgaagcag ctgttcgtgc
     7561 agctgaggct gtatcttatg aaaatgcagg aacaattgag tttttattag atgagaatag
     7621 cggacagttt tatttcatgg aaatgaatac tcgtgttcaa gtagaacacc ctgttacaga
     7681 atttgtaacg ggtgttgaca tcgttaaaga gcagattcgt atcgctgctg gaattccttt
     7741 atcagtttct caaaatgata ttaagttaac tggtcatgcg attgaatgtc gtattaacgc
     7801 agagaatcca caatttaatt ttgcgccatg tccaggtacc attaatgggt tacatctacc
     7861 tgctggcggt atgggcttac gtgtcgattc agcggtttac acaggctaca ctattcctcc
     7921 ttattatgat agtatgattg cgaaagtaat tgttcacgga gaaaatcgtt ttgatgcact
     7981 tatgaaaatg caacgtgcct tatatgagct tgaaattgat ggtattataa caaatacaga
     8041 atttcaaatg gatctcattt ctgataaaaa agtcttggct ggagattatg atacttcatt
     8101 cctgatggaa gactttttac caaggtatta tggtaacaat caataggagg gaagatggct
     8161 ttatttagta aaaaggataa atatatccgt attagtccaa acaaagcatt agggtcttct
     8221 gataaacgta gtcttcctga ggttcctgat gaactctttg ctaagtgtcc ttcttgtaaa
     8281 catatgattt atcagaaaga tttaggatta gcaaagattt gtccagcttg ttcttacaat
     8341 tttagaattt cagcgcaaga acgtctgttg ctaacagttg acgaagatag ttttgaggaa
     8401 ttatttacag ggattgaaac taaggatcct ttgaattttc caaactaccg tgaaaaactg
     8461 gctgctacac gtcaaaagac taacttagat gaagcggtgg tgacaggttt agctaaaata
     8521 aaaggacaaa ctacggcctt agctattatg gattctcatt ttataatggc gtctatggga
     8581 acggttgtag gcgaaaagtt gacaagatta tttgagctag caactgaaaa gaaattacct
     8641 attgttattt ttacagcgtc agggggcgcc agaatgcaag aaggtattat gagtttgatg
     8701 caaatggcaa aagtatcggc agcagttaaa cgccattcta accagggatt gttttattta
     8761 acaattttaa cagacccaac aaccggcggc gttactgcga gttttgctat ggaaggtgat
     8821 attattctgg ctgagccgca agcgttggtt ggttttgctg gtcgtcgtgt tatcgaaaca
     8881 acggtgagag aagatttacc tgaaggcttt caaaaggcag agtttttgtt agaacatggt
     8941 tttgttgatg ctattataaa tcgaacagaa ttacgcgact gcattgctca actaattgct
     9001 ttccatggaa atgaggttgg tcatgaataa tgatgtgaca agaattttaa aagatgcgcg
     9061 tgatcaaggg aggctaactg ctcttgacta cgctgaattg atttttgata attttatgga
     9121 acttcatggt gatcgccagt tcgcagatga caaatcaatt ataggaggtt tggggtactt
     9181 ggcagggcgt cctgtgacga ttgtcggtat tcaaaaaggt aagaaccttc aagataattt
     9241 agaccgtcat tttggtcaac cacatcctga aggttatcgt aaggctttac gtttaatgaa
     9301 acaagctgag aagtttggaa gacctgttat aaccttcatc aatactgctg gagcatatcc
     9361 tggggtaggt gctgaggagc gaggtcaggg tgaagcaatt gctagaaacc tcttagaaat
     9421 gagtgattta aaagtcccta ttatagctat tattattggt gaagggggtt ctggaggagc
     9481 attagcttta gctgttgccg ataaggtttg gatgttggaa catactgttt attctatttt
     9541 aagtcctgag ggctttgcct ctattttatg gaaagatggt acaagaacaa cagaagcagc
     9601 acaattaatg aagatgactg ctggagaact ttaccatatg gaagttgtag acaaagtaat
     9661 ccctgaacat ggttattttt caagtgagat tgtcgatatg attaagacaa gtttaatttc
     9721 agagttagaa atgctctcac aattatctct tgaggattta ttggagcaac gttaccaacg
     9781 ttttagaaaa tattaataga aaaaggctga gttatttact cagcctatat ttagtttttc
     9841 aaggtattat catagttata gtttcatatt ttgctataat tgataaaaag gagatatgtc
     9901 aatgacacta gcattagcca atttttagat tgtagtctaa tccaaaattt tcagaaagag
     9961 tcatttcgaa cactttattc taagtaccat tatttaggct ctccttaact tgagactcta
    10021 tctgttattg aagagaaatt ttctagaaaa aatagctatt gttagatttt agaaggctat
    10081 gaaaagattg gtttttaaag agttctatta agtgatgaag aaagtaaaac ttggttagtt
    10141 ataattgcga ttagactttg tttctagcaa cgagacttcg ctatacatac tttaaaagaa
    10201 cttgaaagta tgtatagata tataatcgtg aaaacttaac tattgtttac atggagaaaa
    10261 agaatgtatc aagaattaaa tgattattta gaattccaat caatgaagta tattatgcct
    10321 gagaaggcag gccctttaaa ggacgatatg caaactttta aggcaaaggg ccaacttgct
    10381 aggaatagct ttacagagtt atcaagagcc ttagaacaac gtatggatgg ctttaaaatg
    10441 caaagagtga gtaattgggc taatcaagct caagtagggc gtccacattt ttgggtttat
    10501 tatcgaaagg atacagacca attagatgat gtggctgttg cgctaagggt ttatggtgta
    10561 aaagatagtt ttggggttag tcttgaggta agctttgtag agaggcaaaa atctgataag
    10621 acactcgaga agcaggctag agtgttatcg attccgattg cctctccatt gtattttatg
    10681 gtccaaaggc aaggtgaaac tcatcgagag gaaggcaatg aggaaaatcg tcaacgttta
    10741 atgcaagaaa ttaagtcagg gaaggtaagg aaagtattgg taaaatatga tgttcttttg
    10801 actgaaaacc agagcttaga aaatattctg caacggttgt tagaaggatt tgagaaagtg
    10861 ctaccttact accaagtttg tcaaactatt agctaagttc atggaaaaaa tgagttggta
    10921 taccaactca ttttagagtg cctaatttgg tttaataata tcgatattac ccatatatgg
    10981 acgaagaact tctgggatag tcacagaacc atcttcattt tgataatttt cgaggattgc
    11041 tgctacagta cgtccaactg caagtcctga accatttaaa gtatgaagca acctaacttt
    11101 gccatcaact tcgtcacggt aacgaatttg agcacgacgt gcttggaaat cttcagtatt
    11161 agaacatgaa gaaatttctc gataagtatt ttgagcggga atccatacct ctaagtcata
    11221 cgttttagct gctgagaatc ccatatctcc tgtacacaag gtaatgacac ggtatgggag
    11281 attaagtttt tgaagaatat tttcagcatt tgctgtcatt ttttctaatt cttggtacga
    11341 ttcttcaggc ttggcaaatt tcaccatttc tactttatgg aattgatgaa ggcgaattaa
    11401 tccacgtgtg tcacgtcctg cagagcctgc ttctgaacgg aatgatggac tcatggcagt
    11461 aaagtatata ggtaattctt tcccatcgat aatttcatca cgataatagt ttgttaaagg
    11521 aacttcagct gttgggataa gtacgaatgg gctatctgct aattcgaaag tatcttcctt
    11581 gaactttgga tattgtcccg taccgaacat tgaatcgtgg ttaaccatat aaggagggat
    11641 cacttcagtg tacccttcct tagcgtgttc atccagcatg aagctgtaga tagcacgctc
    11701 taaacgagca cctagacctt tgtagaagag gaagcgagaa ccagtaactt ttgcaccacg
    11761 ttcccaatcg agaattccaa ggctttcccc taaatcccag tgagctttgg cctcaaaatc
    11821 aaattcacga ggagttcccc agcgacgaac ttcaacattt tcatcctcgt ctgctccaag
    11881 cggcacatca tcagctggtg tattaggaag tacggtcacc attgattgta aattagcatc
    11941 cacatctgcc aattccgcat caattgcttt gatatcagcg gaaagtgttt gcatagctgt
    12001 gatttgctcg tctgcattct ctttatttcg ttttgcttga gcaatggcag cagaagcaac
    12061 attacgttgc gctttcgcct cttcagattt gataagtaat tcacggcgtt ttatatcaag
    12121 ttctttaagg gttgttaatg tttcttggtc aacaccacgc gtggccaatt ttttagcgac
    12181 agcatcaaaa tctgtacgaa tacgttttaa gtctaacata taggaatctc ctttatacta
    12241 aaaaaaacac cctgtcatgc tggagcgcaa aggcgcggtt ccatccagct tcacagaagt
    12301 gcacttgatt gattgattaa attatttccc ataaatggaa cacggtagaa tttcataaaa
    12361 caatcttatc tgctcgcaac gaccgcagac tctctgaaaa gaactattat actacttatc
    12421 cgttaaggtt attatatcca aaaataagta aaatgtaaac cttaacttta taagcctgct
    12481 ctttgtttta gtgaaatagc tatgctaaaa tatgattatg gaaaagtata caagtattaa
    12541 acaatcaggt cgtcattacg gcttagacct tttaaggatt atttccatgt tcatgattgt
    12601 tattacgcat gttcttggaa aaggtggtct gagatcttcg gttgagggac acgcagactc
    12661 ctattttata gtaacgtgga ttatccaagt cttagtttat ggggccgtta actgttacgc
    12721 actaataagt ggttatgtgg gaattaattc gcgttatcgt tactcaaaat tactgtctat
    12781 ctggacacag gtttttttct acacctttac aataacagca ctttttgcaa tcacaggtca
    12841 tgaagtgacc ttgcttaatt ggagagatgc ttttttccct attgtcagtg ggcagtattg
    12901 gtacattact gcttactttg gtctattggt ttttatgcct gttattaata atggattaaa
    12961 tgccttgact gataaacaat taaaacaatt ggttttgtta atgtttatta ttttttcaat
    13021 tttaccagct gttttaaaca atcgtgttcc agaattttca ttgagcaagg gttttgaaat
    13081 gacttggcta ctgattttat atattattgg tgcctatttg aaacgtattg atttaaatat
    13141 atttaaaaca tcatacttac ttatagttta tctattatca ttggttgcta cttatgtcat
    13201 gaagtttagt attggtgata tttggtattg gtatgtttcg cctactttaa ctttaggggc
    13261 ggtaagtttg tttatattat ttgctagagc aagcattaag ccctcgggat tcttaaagaa
    13321 aataattgtt gtattagccc cttcaacctt gggagtttat ctctgtcatc tccacccact
    13381 aatagtaaaa tattttgtaa gagattttgc agagacgttt gtttatgagt ccatttatct
    13441 ttacccattt ttaattttag gagcagggat tttaatttat ttgttggctt ttattgtaga
    13501 acgtattaga ttacgaatat ttaaagcaat ccatttttct aactttttga gatggtttga
    13561 tg
//
LOCUS       DATROD010000040        13372 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.040, whole genome shotgun sequence.
ACCESSION   DATROD010000040 DATROD010000000
VERSION     DATROD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13372
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.040"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..1329)
                     /gene="brnQ"
                     /locus_tag="VBC74_001114"
     CDS             complement(<1..1329)
                     /gene="brnQ"
                     /locus_tag="VBC74_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO6942070.1"
                     /translation="MVKKGFLTGLLLFGIFFGAGNLIFPPALGVASGQDFWPAILGFC
                     LSGVGLAIITLLLGTLTNGGYKTEMSEKFSPWFSLTFLVILYLTIGPLFAIPRTATVS
                     FEIGVAPIVGHSPIALLCFTACFFAAAYYLAIRPNGILDSVGKILTPVFAFLILSLVV
                     VGAIAYGNLESAKASADYAGKAFGSGVLAGYNTLDALAAVAFCLVATETLKKFGFKTK
                     KEYLSTIWIVGIVTSLAFSILYIGLGFLGNKFPVPADILADPNVNKGAYVLSQASYKL
                     FGNFGRYFLSIMVTLTCFTTTVGLIVSVSEFFDKNFRFGNYKLFATVFTLIGFLIANL
                     GLNAVITFSVPVLTLLYPIVIVIVLIILINKWLPLSKKGMSLTIGLVTLVSFVEVLAG
                     QWQEKTLTQLVGFLPFHTISMGWLVPMLIGIVFSLVLSDKQKGQAFDLEKF"
     gene            complement(1525..3522)
                     /gene="metG"
                     /locus_tag="VBC74_001115"
     CDS             complement(1525..3522)
                     /gene="metG"
                     /locus_tag="VBC74_001115"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEO6942071.1"
                     /translation="MTEKKSFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRMMGFD
                     VQYLTGLDEHGQKIQQKAEEAGITPQEYVDGMAESVKTLWELLDISYDKFIRTTDTYH
                     EEAVAKIFEQLLAQGDIYLDEYTGWYSVSDEEFFTESQLAEVYRDENGNMIGGVAPSG
                     HEVEKVSEESYFFRMSKYADRLKAYYAEHPEFIQPDGRMNEMLKNFIEPGLEDLAVSR
                     TTYTWGVQVPSNPKHVIYVWIDALMNYISALGYGWSDDLSQYHKFWPADIHMIGKDIL
                     RFHSIYWPIMLMALDLPLPKRLVAHGWFVMQDGKMSKSKGNVVYPEMLVERFGLDPLR
                     YYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTIAMVNKYFDGEVPRFAVAT
                     DFDADLASVATDSIENYHKQMEAVDFPRALEAVWNLISRTNKYIDETAPWVLAKDETD
                     RDKLAAVMSHLVASLRVVAHLIQPFMMETSDAIMEQLGLGATFDLEKLTFADLPEGVR
                     VVAKGSPIFPRLDMEDEITYIKEQMNAGKAPVEKEWVPEEVELTSSKGQIKFEDFDAV
                     EIRVAEVIEVEKVEGSDKLLRFRLDAGDEGHRQILSGIAKFYPNEQELVGKKLQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTVLTVDSAVANGSIIG"
     gene            3665..4540
                     /gene="tehB"
                     /locus_tag="VBC74_001116"
     CDS             3665..4540
                     /gene="tehB"
                     /locus_tag="VBC74_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEO6942072.1"
                     /translation="MVNTKTLLPYKTMPVWTAQSIPKAFLEKHNTKEGTWAKLTILSG
                     SLVFYQLSPDGEEISRHIFDASSDIPFVDPQVWHKVSPNSPDLSCYLTFYCQKEDYFH
                     KKYGLTRTHSEVIASAPLLSEKSNILDLGCGQGRNSLYLSLLGHQVTSVDSNGQSLVA
                     LENMALEEELPYNIKRYDINTAAIEGHYDFILSTVVFMFLNPDCISDIILQMQSHTQI
                     GGYNLIVSAMDTAENPCPLPFPFTFKEGQLKSYYNDWEIIKYNENLGELHRVDENGNR
                     LKLQFATLLARKLRS"
     gene            complement(4698..5393)
                     /locus_tag="VBC74_001117"
     CDS             complement(4698..5393)
                     /locus_tag="VBC74_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO6942073.1"
                     /translation="MSKKSHRQYQIYEGLRCAVALCFISGYINAFTYVTQGKRFAGVQ
                     TGNLLSFAIHLSNKHYSQALAFLLPIMVFMLGQSFTYFMNRWANKHQLHWYLLSSFAL
                     TQVAIVTIILTPFLPSSFTVAGLAFFASIQVDTFKSLRGAPYANMMMTGNIKNAAYLL
                     TKGLYEKNSDIFLIARNTIIIIGGFIFGVVCSTYFSSKLGEWSLSLILIPLLYVNLLL
                     GHEFYNLQVERHS"
     gene            5729..6946
                     /locus_tag="VBC74_001118"
     CDS             5729..6946
                     /locus_tag="VBC74_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6942074.1"
                     /translation="MHSTIYKCHHCRYLIYKSFKDIFPFALIFSWIEILQKLVISDDS
                     FLAQTTKSSIYKNEWLYTVSNTLYDLSHLLIYFMASYFVASLVWRGLSNQTKESFSAS
                     LLGFATVWLLLTSVVPGTDYHYPSQPIWLMLALISYLFIFLTRLLQNRHHIFQYSMLA
                     ILLLISSILSHILYSYPNIDLEYLIQNHFSQWLGDGPHQLIPIMLWSILAIFLTLSGL
                     FVPDILFRPYSYFSVVSENLNAALSQHTDKIPYLYTFYTVKNSFAMFGGIGILLSLFL
                     AVLYESRKLQSKNYYKLTLLTLTPLIFDQNLPFLVGLPVILQPILFIPMVLTTIFAEA
                     FGALMLYLKFVDPAVYTVPSGTPSLLFGFLASNGDWRYLPVTAIILVVGFFIYRPFVK
                     IAFAKEEQYEKIT"
     gene            6930..7772
                     /locus_tag="VBC74_001119"
     CDS             6930..7772
                     /locus_tag="VBC74_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6942075.1"
                     /translation="MKKLLRWLPPVLFIIILIGMTILGKSYINKVTAHKIKLYNSRMT
                     PTILIPGSSATQERFNSMLAQLNQMGEKHSVLKLTVKKDNSIIYNGQISGNDHKPYIV
                     IGFENNEDGYSNIKKQTKWLQIAMNDLQKKYKFKRFNAIGHSNGGLSWTIFLEDYYDS
                     DEFDMKSLLTMGTPFNFEESNTSNHTQMLKDLISNKGNIPSSLMVYNLAGTNSYDGDK
                     IVPFASVETGKYIFQETAKHYTQLTVTGNNATHSDLPDNPEVIQYVAEKILKNEKGKL
                     PKPH"
     gene            7849..8676
                     /locus_tag="VBC74_001120"
     CDS             7849..8676
                     /locus_tag="VBC74_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEO6942076.1"
                     /translation="MKLISWNIDSLNAALTSESTRALMSRQVIDTLVAEDADIIAIQE
                     TKLSAKGPTKKHLEVLETYFPEYDLVWRSSVEPARKGYAGTMFLYRKGLNPIVSFPEI
                     DAPTTMDNEGRIITLELENCYITQVYTPNAGDGLKRLADRQIWDIKYAEYLATLDSQK
                     PVLATGDYNVAHKEIDLANPSSNRRSAGFTAEERQGFTNLLAKGFTDTFRYLHGDVPN
                     VYSWWAQRSRTSKINNTGWRIDYWLTSNRVADKITKSEMIHSGDRQDHTPIILEIEL"
     gene            complement(8716..9072)
                     /locus_tag="VBC74_001121"
     CDS             complement(8716..9072)
                     /locus_tag="VBC74_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEO6942077.1"
                     /translation="MYTFYEYPKCTTCRSAKKELTELGLTFEAIDIKSNPPKASLLKE
                     LLENSPYDLKKFFNTSGNSYRELGLKDKFDDLTLDQALDLLASDGMLIKRPLLVKDNK
                     ILQIGYRTKYKDLNLV"
     gene            complement(9074..9550)
                     /locus_tag="VBC74_001122"
     CDS             complement(9074..9550)
                     /locus_tag="VBC74_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEO6942078.1"
                     /translation="MLYQEFYQSPLGEIRLLADNLGLSGLYFVGQKYDMLAVNQEEIV
                     NMSNSYTLLGKKWLDAYFSQQNLPSIPLSLRGTAFQTRVWQELQKIPFGDTKTYGELA
                     KELNCQSAQAVGGAIGKNPISLIIPCHRVLGRYGQLTGYAGGLERKSWLLEYEKEK"
     gene            complement(9605..10755)
                     /locus_tag="VBC74_001123"
                     /pseudo
     CDS             complement(9605..10755)
                     /locus_tag="VBC74_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321778.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
     gene            complement(10828..11376)
                     /locus_tag="VBC74_001124"
     CDS             complement(10828..11376)
                     /locus_tag="VBC74_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO6942079.1"
                     /translation="MQIRLAFPNEIDQIMLLIEEARAEIAKTGSDQWQKEDGYPNRND
                     IIDDILNGYAWVGIEDGMLATYAAVIDGHEEVYDAIYEGKWLHDNHRYLTFHRIAISN
                     QFRGRGLAQTFLQGLIEGHKGPDFRCDTHEKNVTMQHILNKLGYQYCGKVPLDGVRLA
                     YQKIKEKGETSIYREIDERNPM"
     gene            complement(11444..12535)
                     /gene="serC"
                     /locus_tag="VBC74_001125"
     CDS             complement(11444..12535)
                     /gene="serC"
                     /locus_tag="VBC74_001125"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEO6942080.1"
                     /translation="MIIYNFSAGPAVLPKPVLVKAQSELLNYQGSSMSVLEVSHRSKE
                     FDDIIKGAERYLRDLMGIPDNYKVIFLQGGASLQFSMIPLNITRGRKAYYHVAGSWGK
                     KAYTEAVKLSKTIPFEPILLASSEESVYDYIPEFDEKEIDPKAAYVHVTTNNTIEGTS
                     LYDIPKTNGVPVIADMSSNILAVKYKVEDFAMIYAGAQKNIGPAGVTVVIIREDMINE
                     EPTLSSMLDYKVQSDAGSLYNTPPAYSIYIAKLVFEWVKSLGGVDAMEKANREKSGLL
                     YDYIDSSEFYSNPVRDKKSRSLCNIPFITINKDLDEKFVKEATERGFKNIKGHRSVGG
                     MRASLYNAFPKQGVIELIDFMKTFEAENT"
     gene            12668..13303
                     /locus_tag="VBC74_001126"
     CDS             12668..13303
                     /locus_tag="VBC74_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEO6942081.1"
                     /translation="MILREFCAENLTDLTRLDKAIISRVELCDNLAVGGTTPSYGVIK
                     EANQYLHEKGISVAVMIRPRGGNFVYNDLELRIMEEDILRAVELESDALVLGILTSNN
                     HIDTEAIEQLLPATQGLPLVFHMAFDVIPKSDQKKSIDQLVALGFTRILLHGSSNGEP
                     IIENIKHIKALVEYANNRIEIMVGGGVTAENYQYICQETGVKQAHGTRITQ"
BASE COUNT         4391 a         2553 c         2101 g         4327 t
ORIGIN      
        1 aaatttctct aaatcaaatg cttggccttt ttgtttatct gacagcacca aagaaaatac
       61 tataccaatc aacattggaa ctagccaccc cattgaaata gtatgaaagg ggaggaatcc
      121 aactaactga gttaatgttt tttcttgcca ttgtcccgct agaacctcaa caaacgacac
      181 taaagtgaca agaccaattg tcaaagacat tccctttttt gaaagcggca accacttatt
      241 aatcaaaata attaagacaa taacaataac aatcggatac aataaagtta gcacaggaac
      301 tgaaaaagta attacagcat ttaaaccaag attagcaatt aaaaagccaa tcaaagtaaa
      361 aacagtagca aataatttat aattcccaaa acgaaagttc ttatcaaaaa attcagatac
      421 tgacacaata agcccaactg ttgttgtaaa gcatgtcaat gttaccataa tactcaagaa
      481 ataacgacca aaatttccaa ataatttata agatgcttgt gataatacat aagctccttt
      541 attaacattt gggtcagcta aaatatcagc tgggacagga aacttattcc ctaaaaaacc
      601 taagccaata tataaaatac taaatgctaa actagtaaca attccaacaa tccaaatggt
      661 tgataaatat tcttttttag ttttaaaacc aaacttttta agcgtttctg ttgctactag
      721 acaaaaagca actgctgcaa gtgcatcaag tgtattatag cctgctaaga caccacttcc
      781 aaacgctttt cctgcatagt cagcactcgc cttagcactc tccaagtttc cataagcaat
      841 agctccaaca acaaccaaag agagaattaa aaaggcaaaa acaggcgtta agatttttcc
      901 aacactatct aatataccat ttggtctaat agcgagataa taagctgcag caaaaaaaca
      961 ggctgtaaaa cacaaaagag caatcggact atgccctaca attggagcaa ccccaatttc
     1021 aaaagaaacc gttgctgttc taggaatagc aaaaagtgga ccaatagtta ggtagagtat
     1081 aactaaaaat gttaaagaaa accacggact aaatttttcc gacatctctg tcttataacc
     1141 accattagtt agtgtgccta gtaggagggt aataatagct aatccaactc ctgacaaaca
     1201 aaatcctaaa atcgctggcc agaaatcttg tccagatgct acacctaatg ctggtggaaa
     1261 aatgaggttc cctgcaccaa agaagatacc aaataagagt aatcccgtta aaaagccttt
     1321 ttttaccata agcaaccatt agtgtaaata atctatttac accctttcca taaattctcc
     1381 ttatcaaatt ctatctaaat gcttagaatc gatgtctttt attatatcaa aaataaaaat
     1441 aaattctagg tcattttcat aattttccaa ctattcagtc acttttttct tgaaaacaaa
     1501 aaaagagcat aatgctcttt tcgattaacc aataattgat ccatttgcca ctgctgaatc
     1561 gaccgtcaac actgtcaatt taccatcatg ttctgctgag agaatcatgc cttgactgac
     1621 atattttttc atcattttac gtggtttgag gttagcaaca atttggagtt ttttccctac
     1681 taactcttgt tcatttgggt aaaattttgc aatccctgat aagatttgac gatggccctc
     1741 atcaccagca tctagacgga aacgaagaag tttatcagaa ccttccactt tctcaacttc
     1801 aattacttct gcaacgcgga tttcaacagc gtcaaaatcc tcaaacttga tttgaccttt
     1861 gcttgatgtc aattcaactt cttcaggaac ccactccttt tccactggtg cttttccagc
     1921 attcatttgt tctttgatat aagtaatttc gtcttccata tccaaccttg ggaaaattgg
     1981 tgaacctttt gcaacaactc gaacaccttc tggtagatca gcaaaagtca atttttcaag
     2041 atcgaatgtt gcccctaaac caagttgttc cataatggca tcggaagttt ccatcatgaa
     2101 cggttgaatg aggtgagcaa caacacgtag actagcaact aaatgactca ttacagctgc
     2161 taacttatca cgatctgttt cgtcttttgc aagtacccac ggtgctgttt catcaatata
     2221 tttgttagtt cgtgaaataa gattccatac agcttcaagg gcacgtggga aatccaccgc
     2281 ttccatttgt ttatgatagt tttcaatact atctgtcgca acagaagcta aatcggcatc
     2341 aaagtcagtt gcaacagcaa accttggtac ttctccatcg aaatacttgt taaccatagc
     2401 aatagtgcgg ttgagaaggt ttcctaaatc attagcaagt tcatagttaa tacgtccaac
     2461 ataatcttct ggcgtaaaag ttccgtctga accaacaggc aatgaacgca tgagataata
     2521 acggagtgga tcaagaccaa aacgctctac taacatttct ggataaacga cattcccttt
     2581 tgatttagac attttaccat cttgcatcac aaaccaacca tgagcaacta gacgttttgg
     2641 taatggcaaa tccagcgcca tcaacatgat aggccaataa atagagtgga aacgaagaat
     2701 atctttacca atcatatgaa tgtctgctgg ccaaaattta tgatattgac tgagatcgtc
     2761 agaccaacca taaccaagag cagaaatata gttcatcaaa gcatcaatcc agacataaat
     2821 aacatgtttt ggatttgatg gtacctgaac tccccatgtg taagttgtac gagacactgc
     2881 taaatcttct aaacctggtt ctataaagtt tttaagcatt tcattcatac gaccatctgg
     2941 ctgaataaat tctggatgct cagcgtagta cgcttttaaa cgatctgcat atttgctcat
     3001 gcggaagaaa taagattctt cagaaacttt ttcaacttca tgaccagatg gcgcaacacc
     3061 accaatcata ttaccgtttt cgtcacgata aacttctgct aattggcttt cagtaaaaaa
     3121 ttcttcatct gaaacagaat accatccggt atactcatca agatagatgt ccccttgtgc
     3181 caacaattgt tcaaaaattt tagcaaccgc ttcttcatgg taagtatctg ttgtacggat
     3241 gaatttgtca taggaaatat ctaaaagttc ccataatgtt ttgacacttt cagccatgcc
     3301 atcaacatat tcctgtggtg taatgcctgc ttcttcagcc ttttgttgaa ttttctgacc
     3361 atgctcatca agaccagtca aatactgaac atcaaagccc atcatgcgtt tgtaacgtgc
     3421 caaaacatca catgctatcg ttgtataggc agaaccgatg tgcaatttac ctgatgggta
     3481 ataaattggg gtagtaatgt aaaatgattt tttttctgtc atgatttctc ctataagcca
     3541 ccctaatgag aggtgttttt tatcatgata cattataaca taaaatcttt ttcaactgga
     3601 tatatctctg aataatatat ctacttttgc tataataaag gtattaaaaa tagagagtta
     3661 atatatggtt aatacaaaaa cacttctccc ctacaaaact atgccggttt ggactgcaca
     3721 gtctattcca aaggcatttt tagaaaagca taatactaag gaaggcacct gggcaaaact
     3781 aaccattcta agtggttctt tagtatttta ccagttatct cctgatggag aggaaatctc
     3841 gcggcatatt tttgatgcta gtagtgatat tccttttgtt gatccacaag tctggcataa
     3901 agtttcgccg aatagtccag acttaagttg ctatctaact ttttactgcc aaaaagaaga
     3961 ttacttccat aaaaaatatg gtctcacgcg cacacattct gaggttatcg ccagtgcacc
     4021 tctcttatct gagaagagta atatattaga ccttgggtgt ggtcaagggc gaaactcact
     4081 ttatttatcg ctgctgggac atcaagtgac ttctgtcgat tcaaacggac agagccttgt
     4141 agctttagaa aatatggcat tagaagaaga gcttccttac aatataaaaa ggtatgatat
     4201 taatactgct gctattgaag ggcactatga ttttatttta tcaactgtgg tatttatgtt
     4261 tttaaatccc gactgcattt ctgatattat tttacaaatg caatcacata ctcagattgg
     4321 aggatacaat cttatcgttt ctgctatgga taccgctgaa aatccttgcc cgcttccatt
     4381 cccttttacc ttcaaagaag gacaattgaa atcttattac aatgactggg aaatcatcaa
     4441 atataatgaa aatttaggtg aattgcacag ggtggatgaa aacggtaatc gcttaaaatt
     4501 acagtttgcg actttattag ctcgaaaatt aagatcataa aagtgagtag aaatctactc
     4561 acttttatct ttggttaggt aataagtata ttctaaaccc atacataatt atcctataaa
     4621 atatatagag atgatatttc aaatcattat tgtaacaatc agctttagta tggctcttca
     4681 acataaaacg ccatacatta ggagtgcctt tctacttgta ggttataaaa ttcatggcct
     4741 aacagcaagt tgacatataa aagaggaatt aatattaatg ataagctcca ttcgcccagt
     4801 ttacttgaaa aataagtcga gcaaacaaca ccaaagataa atcctccaat aataataatc
     4861 gtatttctag caattaagaa aatatcagaa tttttttcgt aaagtccctt tgttaataaa
     4921 taagcagcat tcttgatatt accagtcatc atcatattag cataaggtgc tcctctgagc
     4981 gatttaaagg tatctacttg tattgaagca aagaaggcta gtcctgccac agtaaacgac
     5041 gatggtaaga aaggagtcaa tataattgtt actatagcaa cctgtgtcaa cgcaaaacta
     5101 gataataggt accagtgcag ttgatgttta ttcgcccaac gattcatgaa ataagtaaac
     5161 gattgaccta acatgaaaac cattataggt agcaaaaaag caagcgcttg actataatgc
     5221 ttattactaa gatgaatagc aaaagataac aaattccctg tttgtacacc tgcaaaacgt
     5281 tttccttgtg ttacataagt aaaagcattg atataacctg aaataaaaca gagtgcaacc
     5341 gcacacctga gcccttcata tatttgatat tgacggtgag actttttaga catattttcc
     5401 tctaacttta tggcaaacaa aaataagact gggcaatctt gctcagcact ttttttctaa
     5461 tagattggtt taaaatagta ttaatattag cactagctag cctaacagtt ctaaataaat
     5521 tattaagcaa tgataaatgt agttttagta tatcatagcc tcagcattga agcaatctca
     5581 ctttatgaaa ggataaagaa aatctcataa gtcatttacc cttaatttga agtttaatta
     5641 gttactttta attttactac caaaaaactt gcaccattct taacatttgt tacaataaat
     5701 acaacctatt aggaggtcag tacagattat gcacagcact atttataaat gtcatcattg
     5761 ccgttatcta atttataaaa gttttaaaga tatctttcct tttgctctca ttttttcttg
     5821 gattgaaatc cttcaaaaac tcgttatatc agatgattct tttttagctc aaacaacaaa
     5881 gtccagtatt tataaaaacg agtggttata tacggtttcc aatactcttt atgatttatc
     5941 tcatttacta atctacttta tggctagtta ttttgtggct agtttagtat ggagaggctt
     6001 atctaaccaa actaaagaaa gtttttctgc aagtttactt ggctttgcca ctgtttggtt
     6061 gcttctaact tcagttgttc caggtacaga ttatcactat cctagccaac ccatttggct
     6121 tatgctggct ttgatttcgt atctttttat ctttttaact cgactgcttc aaaatcgaca
     6181 tcatattttc cagtatagta tgttagctat actactatta atctcttcta ttttgtcaca
     6241 tatcttatat agttatccta atattgattt agaatacctt atacaaaatc acttttctca
     6301 atggctgggt gatgggccac atcaattaat tcctattatg ctttggtcta tcttagctat
     6361 ttttctaacc ttatcaggtc tttttgtacc agatatttta tttcgtcctt actcctattt
     6421 ttcagtagtt agcgaaaatt taaatgctgc cttgagccaa catacagata aaatccctta
     6481 tctgtatacc ttttatactg ttaaaaatag ctttgctatg tttggcggta ttggaatcct
     6541 attatcctta tttttagcag ttttatatga aagtagaaaa ctacaatcga agaattacta
     6601 taaattgaca ttactgacct taactcctct tatttttgat caaaatttac catttttagt
     6661 aggtctacct gtcattttac aaccaatatt attcattccg atggtattaa caactatctt
     6721 tgcagaggct tttggagctc ttatgctcta cctaaaattt gtcgaccctg cagtttatac
     6781 tgtcccaagt ggtacaccta gtctactatt tggctttctt gcttcaaatg gggattggcg
     6841 ttaccttcca gtcactgcaa ttattctagt tgttggcttc tttatttatc gtccgtttgt
     6901 caaaatcgct ttcgctaagg aggagcaata tgaaaaaatt acttagatgg cttcctcctg
     6961 tacttttcat tattatcctt ataggaatga ctatcttagg taagtcctat atcaataaag
     7021 taacagctca caaaataaaa ctctataact ctcgaatgac tcctactatt ttaattccag
     7081 gatccagtgc tactcaagaa cgatttaaca gcatgttagc acagctcaac caaatgggag
     7141 aaaaacatag cgttttaaag ttaactgtca aaaaagacaa tagcattatc tacaatggac
     7201 aaattagcgg caatgaccac aaaccctaca ttgtcattgg atttgaaaat aatgaagatg
     7261 gttatagtaa catcaaaaaa caaacaaaat ggctacagat tgctatgaat gatcttcaga
     7321 agaaatataa atttaaacgt tttaacgcta tcggtcattc aaatggtggc ttatcatgga
     7381 ctattttcct agaagattat tacgactctg atgaatttga tatgaaatca ttgttaacaa
     7441 tgggaacacc ttttaacttt gaagaaagta acacctcaaa tcatactcaa atgcttaaag
     7501 atttaatcag taataaagga aatattccat caagtctcat ggtatacaat ttggcaggaa
     7561 ctaattcata tgatggtgat aaaattgttc catttgctag tgtggagact ggtaaatata
     7621 ttttccaaga aaccgctaaa cactataccc aactaacagt aactggtaat aatgctacac
     7681 attctgactt gcctgataat cctgaagtta tccaatatgt cgcagaaaaa attcttaaaa
     7741 atgagaaagg taaattacca aaacctcact aaagctgtag aatagcaacg cctctggtat
     7801 tttgatataa tatattgcga attaaactaa ataaaaaagg atattattat gaaactcatt
     7861 tcatggaata ttgattccct caatgcggca ttaacaagtg aatcaacacg tgctttaatg
     7921 tcacgccaag ttattgatac tttagttgct gaagatgccg acatcattgc gattcaagaa
     7981 actaaactgt ctgccaaagg acctactaaa aaacatttgg aagtacttga aacttacttc
     8041 ccagaatatg atttggtttg gcgatcatca gtcgaacctg ctcgtaaggg ctatgctggt
     8101 acaatgttct tatatcgtaa agggttaaat cctattgtta gcttccctga aatcgatgct
     8161 cctactacaa tggataatga aggtcgcatt attactctcg aattggaaaa ctgctatatc
     8221 acacaggtct atactccaaa tgcaggtgat ggcctaaagc gcttagctga ccgtcaaata
     8281 tgggatatta aatatgctga gtatttagca acattggata gtcaaaaacc tgttctagca
     8341 acaggtgact ataacgtagc ccacaaggaa atagacctag ctaatcctag cagtaatcgt
     8401 cgttctgctg gattcacagc tgaagagcgc caaggcttta caaatctact agccaaaggt
     8461 tttacagata cttttcgtta ccttcatggc gatgttccaa atgtttatag ctggtgggca
     8521 caacgaagtc gtacatccaa aatcaacaat acagggtgga gaattgatta ttggctaaca
     8581 agtaaccgtg tcgcagataa aattaccaag tcagaaatga ttcattctgg tgatcgtcaa
     8641 gatcatacac caattatttt ggagattgaa ctataaacca aaaaaagatg attgagttat
     8701 aactcaatca tctttttata ctaagttgag gtctttatac ttagtacggt agccaatctg
     8761 aagaatttta ttatctttaa ctagcaatgg acgcttaata agcattccat ccgatgccaa
     8821 taagtccaaa gcttggtcaa gagttaaatc atcaaattta tcttttaagc ctaattctct
     8881 gtaagaattt cctgaagtat tgaaaaactt tttaaggtca taaggcgaat tctctaacaa
     8941 ttcttttaat aacgaagctt tgggaggatt gctttttata tcaatcgctt caaaagtcaa
     9001 gccgagttca gttaactctt tttttgctga acgacaagtg gtacatttgg ggtattcata
     9061 aaacgtatac atattatttt tccttttcgt attctaataa ccaagatttt ctttctaagc
     9121 caccagcata accagttaat tgaccatatc ttcctaaaac acggtgacaa ggtataatta
     9181 gagaaatagg atttttacct attgctcctc caacagcctg tgcagattga caatttagct
     9241 ctttcgctaa ttctccataa gttttggtat ctccaaaagg gattttctgt aattcctgcc
     9301 atactctcgt ctgaaatgct gtccctctca atgataatgg aatactaggt aaattttgct
     9361 gtgaaaaata agcatctaac cacttcttac caagtaaagt ataggaattt gacatattaa
     9421 caatttcctc ttggttgact gctagcatat catatttttg cccaacaaaa taaagccctg
     9481 ataaacctag attgtctgct agaaggcgaa tttctccaag aggggactga tagaattctt
     9541 gatataacat atgataagca taacttaaaa agccatgaaa agcaattcat gactttttag
     9601 ttctttattg ttgttttgca attaaacgta cacggacgat attctcatca ccttcaaatt
     9661 cttcaattaa agtagaaatc ttgttattat ccgtttcatc taaatctatt agtgtgtagg
     9721 cgtagtcacc ctttgaacgg ttaataatgt tatcaatatt tatacctaac actgatacag
     9781 cagttgaaat tttagccaca atgttaggaa cattcttgtt aattaaagta atacgaaatg
     9841 gtgcggtttg aatttgatgt acattgggga aattaactga attagtgatt tctcctgtct
     9901 ccataaaaca acgaatggtt tgacttgcca taatagcaca atttagctct gcctcatctg
     9961 ttgatccgcc tacatgagga aagactgtaa ttcctttttg gtttaataat tctttgtctc
    10021 caaaatcagt aatatagcgc ttgacaacac cagtttctat cgcttcaaat agctcttggt
    10081 tattgactaa ttctgcacga gcaaagttga taatcgtagt tccttttttc atgattgaaa
    10141 aagcttccgc atcaaaagta tgcttagttt catttgttaa gggaacatga attgtgatat
    10201 agtcacaagt ttcaaaaata tccttaatct ctttaaccct ttgaacatgg cttgaaatat
    10261 tccaagctgt ttcaattgaa atatagggat cataaccaag aactgtcatt cctaagcgtc
    10321 tagcacattc gcaattctag ctccaatggc accaaggccg ataactccca atttttttcc
    10381 tgcaatttca ttaccagcaa aagctttctt tcctgcttca atttgtttgg gaatatctgt
    10441 tccagttaga gtattaaccc atcggttagc tcctaaataa tcacgagctg aaagtaataa
    10501 ggcagcaatg accgcttctt ttacagcatt agcatttgca cctggggtat taaacacgac
    10561 tattccctgt gcacttgcct cttcaatagg aatattattt gttcctgcac ccgccctagc
    10621 aatagctttg aggttacttg ggaaatcctg attatgcaaa ttttgactac gaataatata
    10681 ggcatcagga ttttctgaca tatccccatc aatctggaaa cgattcccta actcttgtaa
    10741 accaatttga ttaatattat taaatgtttt gacactaagg ttatctcttt agtcatcatt
    10801 gaccaaaata acccctttct ttttttctta catgggattt ctttcatcaa tctcacgata
    10861 gatgctcgtt tctccctttt cttttatctt ctgataagcc aatcgtaccc catctagagg
    10921 tactttacca caatattgat agcctaattt atttagaata tgctgcatag tgacattttt
    10981 ttcatgagta tcgcaacgaa aatcaggtcc tttatgacct tcaattaacc cttgaaggaa
    11041 tgtctgtgct agtcctctac cacgaaattg atttgagatc gcaatacgat gaaatgtaag
    11101 atagcgatgg ttatcatgga gccattttcc ctcataaata gcatcataaa cctcttcatg
    11161 accatcaatg acagccgcat aagtcgctag catcccatct tcaataccaa cccaagcata
    11221 accgttcaag atatcatcaa taatatcgtt acgattaggg tatccatctt ctttttgcca
    11281 ttgatcactt cctgttttag caatttcggc acgcgcttcc tcaataagaa gcataatttg
    11341 gtcaatttca tttggaaaag ctaatctgat ttgcattata ttacccactt ttatatatta
    11401 tagactataa taacggttta attttctttc gtgataacat ttactatgta ttttctgctt
    11461 caaatgtttt cataaaatca attaattcga tgacaccttg tttaggaaag gcattataaa
    11521 ggctagctct cataccacct accgaacgat gtcctttaat atttttaaat ccacgttctg
    11581 tcgcttcctt tacaaatttt tcatctaaat ctttattaat agttatgaaa ggaatgttac
    11641 atagagaacg actcttttta tctctgacag gattactata aaattctgag gaatcaatat
    11701 aatcatagag aagtcctgat ttttcacggt tagctttttc catagcatca acaccaccta
    11761 gacttttcac ccattcaaag actaatttag caatgtaaat actgtaagca ggcggcgtat
    11821 tataaagaga accagcatct gactgaacct tataatccaa cattgatgat aaagttggtt
    11881 cctcattaat catatcttca cggataataa ctaccgttac accagcaggt ccaatatttt
    11941 tttgagctcc tgcataaatc attgcaaaat cttctacttt atattttaca gctaatatat
    12001 tagaagacat atccgcaata acaggcactc cattagtttt aggaatatca taaagcgatg
    12061 ttccttcaat ggtattattt gttgtcacat gaacataggc agctttagga tcaatctctt
    12121 tttcatcaaa ctctggaata taatcataaa ctgattcttc tgatgaagct aatagaattg
    12181 gttcaaaagg aattgttttt gatagtttta cagcctctgt ataagctttt ttcccccacg
    12241 aaccagcaac atgatagtaa gctttacgcc ctctggtaat attcaaggga atcatactaa
    12301 actgtaagga tgcaccacct tgtaaaaaga ttactttata attatccgga attcccatca
    12361 aatctcttaa gtaacgctca gcacctttga taatatcatc aaactctttg gagcggtgcg
    12421 atacctccaa aacactcata ctagaccctt gataattaag gagttcagat tgggctttta
    12481 caagcactgg ttttggtaag actgcaggac ctgcagaaaa attataaatt atcatataac
    12541 acctccatat aattgtgtaa gattatagca cagtattttg aatattgtaa agattttttt
    12601 gattaatttt gctttttatt cgtgatataa tggtaaaggc gaacaatcaa gtttaaagga
    12661 gtaatttatg attttgaggg aattttgtgc agagaattta acagacttaa ccagattaga
    12721 taaagctatt atttcacgtg tcgagctttg tgataacctt gctgtcggag gcactactcc
    12781 tagctacggt gttatcaaag aagctaatca atatttacat gaaaaaggta ttagtgtcgc
    12841 tgttatgatt cgtccccgtg gaggaaattt tgtatacaat gatttagaat tacgtattat
    12901 ggaagaggat atcctacgtg ctgttgagtt agaatcagat gctcttgttt tgggaatatt
    12961 aacttcaaat aatcatattg atactgaagc cattgaacaa cttctacctg ctacccaagg
    13021 tttgccactt gtttttcata tggcatttga tgttatcccg aaatctgacc aaaaaaaatc
    13081 aatagatcaa ttagttgctt taggctttac aaggattctc ctacatggtt catctaatgg
    13141 cgaaccaatt atagaaaata tcaaacatat caaagcatta gttgaatatg ctaataatcg
    13201 tattgaaatt atggttggcg gcggtgtgac tgctgaaaac tatcaatata tttgccaaga
    13261 aactggggtt aaacaagccc atggtactag aataactcaa taaaaaattc gaaacagggc
    13321 tataagtttc tttagtatat ttactactaa gttctctcat gttttgtaaa ac
//
LOCUS       DATROD010000041        12776 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.041, whole genome shotgun sequence.
ACCESSION   DATROD010000041 DATROD010000000
VERSION     DATROD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12776
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.041"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(35..1528)
                     /gene="gtfA"
                     /locus_tag="VBC74_001127"
     CDS             complement(35..1528)
                     /gene="gtfA"
                     /locus_tag="VBC74_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEO6942082.1"
                     /translation="MVVYNLNRGIGWASSGVEYAQAYRSEVFRKLGVEAKFIFTDMFQ
                     NENIEHLTRNIGFEDNEIIWLYTFFTDLTIAATSYSLQQLKESFSLPIDRTEKNGKII
                     SFFFKGSSIVVTVMLNDESSNIVQRVEYLMGGKLVRKDYYSYTKMFSEYYAPEDIGPC
                     LYQRTFYNEDGSVAYEENVDGENSIFKFKETILYSKEELVGYMLEKLQLTNSDLILLD
                     RSTGIGQAVLRNKGNAKVAVVVHAEHYNVSATDETTILWNNYYDYQFSNADSIDAFIT
                     STETQTKTLIDQFKKYLNIEPVVYTIPVGSLSKLQRKEWHERKAFSLLTCSRLASEKH
                     IDWLINAVVEANKVIPELTFDIYGEGGERQKLQEIIAKNKANNYIRLMGHKNLSSVYK
                     DYQVYLSGSTSEGFGLTLMEAIGSGLPIIGLDVPYGNQTFIENNLNGYLIPRETPDNP
                     QQISTAFAQYIVALFNSKDICKKHEYSYRIASRFLNDKIIENWSFFF"
     gene            complement(1544..3940)
                     /gene="secA2"
                     /locus_tag="VBC74_001128"
     CDS             complement(1544..3940)
                     /gene="secA2"
                     /locus_tag="VBC74_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000758330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEO6942083.1"
                     /translation="MKKVVVKPKISILNKLRLQRIQKIVNEINNKKVYYAQLSDKELS
                     HQTLILKNRLSSGESLDDILVDAFAVIREANKRILGLFPYDAQVMGAIALHQGTIAEM
                     KTGEGKTLTATMPLYLNALTSKGAILVTTNDYLAKRDALEMGKVYQFLGMTVGINVFD
                     KKEEADATIKREVYESDITYTTAGALGFDYLVHNLASNKLEQFLRPFHFVIVDEADSV
                     LLDIAQTPLIIAGDPRVQSNLYGITNNFVLTLKENDEYIHKAKDKIVYLTEKGVSYAK
                     QYFNISELYNDEYWELNRHINLALRAHCLYKRDYDYVVKNNEVKLLDNATGRVMEGTK
                     LQSGIHQAIEAKEDVALTNESRAIASITYQSLFNMFPRLSGMTGTGKSSEDELIKTYH
                     LPVIVIPTNFPIKRVDLADKIYVSLPEKLQATIDDVKQRHSKGQPVLLISGTVEIANI
                     YSKLLLREGIAHNTLTADNVVKEAQIIKEAGQKGSVTCATVLAGRGTDIKLGEGVREL
                     GGLAVIGTERMPNLRMDWQLRGRSGRQGDPGISQFYVSLEDELIVSHSPEWVIKYLKK
                     YSRKETSNYYMVPRKKRFFYQIVKNAQLRSEDKGVSSREQTIKFGESLRIQRENIYQL
                     RNELIADSSIVVDGVIKIIQDNFNDIANDKDLTEHSLRRYILENLTYKFKYFPDEFDV
                     HNSDDVFKLLIDIFDREFTAKKTKLQSDNEFDNFVRISILKAIDKSWIEEVDSLQQLK
                     GVVTNRGIGQRDIIQEYYKESLNSYYKMGKEIRYSIVKNIMLSTISKASDGSYSIYYV
                     "
     gene            complement(3937..4482)
                     /gene="asp3"
                     /locus_tag="VBC74_001129"
     CDS             complement(3937..4482)
                     /gene="asp3"
                     /locus_tag="VBC74_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEO6942084.1"
                     /translation="MIKKRIQKVLWTDSSNVYLYGSTIKYLEHNRVFFENLRFSTGKT
                     IKKWLSKTNFQANRTLPELTLLKPNHTYELVSKLTTVPEGCVIIQINFYNRQKEKIGF
                     SIIRDHSSDFIVPDDTYFYDISLIGAGCISVEFCYLELFEVIETQEEKDGIELNSYVE
                     SSLPSELELIRTLIAFSKDKK"
     gene            complement(4475..6064)
                     /gene="asp2"
                     /locus_tag="VBC74_001130"
     CDS             complement(4475..6064)
                     /gene="asp2"
                     /locus_tag="VBC74_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEO6942085.1"
                     /translation="MEKLKILQIGEEPWDADLITPDNIEWLYCNGNAIQIFLEKLKSK
                     ELEKNNVEYIGKTSTNEVKLWFNAVILTSEVSELQLNLLSDCLEAHTLFYDEDFQLDF
                     CSENGIFKRKVLRPLPFNGSRKEKIIFLSRTLFSEQYGAKLKVSDIDLNPDFKGEIIY
                     EGNSSICFSGNFGEDFQPLFSFRYNLSSTDKAIEIWQEYQKLQGDNSIMIEVIQYQKG
                     SLDLILNTKYLSENDLADPYILEYDESVGFSSISIFARGDGVVRFGALHWRYSRKGLG
                     QFLIGGERYSDSKRQEFIYYFNPGDMKPPLNVYFSGFRGAEGFEGFYMMKRLGAPFLL
                     IGDPRIEGGSFYLGTSEYESELENVLQKYLDYLQFDHCQLILSGLSMGSFGALYYGST
                     FSPHAIVVGKPFTNLGDMVVNLKLRRPDDFETSMDILQNIIGSQNDEAINQFNQKFWN
                     KFKRSNFEKTQFAISYMKHDDYDRNATPRLIDYFSKTNTLLYASGYAGRHNDNSQSIN
                     NWFTSQYKTILSDDFGRVFSD"
     gene            complement(6066..7643)
                     /gene="asp1"
                     /locus_tag="VBC74_001131"
     CDS             complement(6066..7643)
                     /gene="asp1"
                     /locus_tag="VBC74_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEO6942086.1"
                     /translation="MFYFIPSWYNSDQKWHNEAPIWYRVLENMTFDDTVSQVRMFQNA
                     DEEVRLLLFSYNPQLRYMLHEESMLAVSYWSFFDDIQNIQQKEIKLINFKELNWPKGT
                     YFVYTPFLVLAKHGGEIIARIQFAENGNLLLIELLIDGVLDKIMIFDDRGFVSSIKYF
                     KQNRLIYQDYLNQNGVWQIREYASKFPRIVVNPMSDKVFNFTEYDDWDTLIRERILVL
                     KKLEVTPDDYIIVAVNDIHNNLVRKSFPDQKKIFSFFGERYSFDNRLDFNELLDEGEL
                     FIAESEKIERLLITSLQEKNYSSNYVENHISRIPPFDTRLRLGQSQNIEELIAYLLVD
                     DLSQTYLSEVLDQLLAKMEVNPSLELKIVSYKNDYDMTSLKEQVKDKILSSKNPDYFF
                     TSLSLEDENHFKEEQKTKIRGISFDIYTNENQIIDALDTARLVIDLRDDPDLYTQIAA
                     LSSGIPQINKVHTNYVEHQKNGWIISDSTELNNAIDYYFDGLMNWNKSLVYSVKKMGD
                     YTSGKILQRWRKLLEKE"
     gene            complement(7653..8906)
                     /gene="secY2"
                     /locus_tag="VBC74_001132"
     CDS             complement(7653..8906)
                     /gene="secY2"
                     /locus_tag="VBC74_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEO6942087.1"
                     /translation="MIGKLYYSYRKSTLLRSILWTILIVGVYMLGQRVLLSTVPLSHQ
                     EIKLAVDQHLLNNFSAVSGGSFNKLNVFTLGLSPWMSSMIIWRFVSLFSWAKNATKRK
                     AEVAQYTLMLTISVIQAYGVSGNQFIKSSLLGSYSDIVFKALTMVLLISGSFVLMWLA
                     NLNASKGLGGSIVIILVSMLQATTTGIITYFEQSHFSIMDLLGTIIAISIILSVLLLI
                     STVVYRAEYRIPIRRIDIVSKFAQDTYIPIRINPAGSYPFMYGMTLMMLPTFIIQALL
                     LAFPNERLLIQISSMIAINKPLGIGLFVILLFILSIGFAYFNYDPYNIAKNMRQRGEY
                     FQNVTPGAATQRFLQEKISIMAVVGAIFTVIMGGLPMLAVVGKTNQVSIAMIVINIYI
                     ISTFMLSIIEQVNTLKLWGSHRNII"
     gene            complement(9016..12435)
                     /gene="srr2"
                     /locus_tag="VBC74_001133"
     CDS             complement(9016..12435)
                     /gene="srr2"
                     /locus_tag="VBC74_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086874280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin Srr2"
                     /protein_id="HEO6942088.1"
                     /translation="MLKKQFGNFGEKSRKVRVKMRKSGKHWVKSVMTQIGYVILSRFS
                     GKEKSSKVQTTSEDLSRTKTSASILTAVAALGAVVGGTTDTTSVSAEETPTATELTGN
                     EKTLATAETVVVAPEVKTVNSDSSSHSTSESQSMSTSTLQSTSASLSASESLMDSTSA
                     SLSESSSLSEYSSLSLSSSESVSASESVQSSEAATTARVQPRAMRVVSSASDMETLPA
                     ALISGEGDVTTVQGQDVTDKLQNLDIKLSGGVQAKAGVINMDKSESMHMSLKFTIDSV
                     NRGDTFEIKLSDNIDTNGASNYSIVEPIKSPTGEVYATGIYDSQKKSIVYSFTDFAAS
                     KNNINGILDIPLWPDDTTVQNTKEDVLFSVKIKDQEATIKETVKYDPPVRIDFAGGVS
                     VDSRITNIDDVGKKMTYISQINVDGKSLYNYNGLYTRIYNYSKESTADLKNSTIKIYK
                     TTSDNIVESMVQDYSSMEDVTSKFANSYPEKGWYDIYWGQFIASNETYVIVVETPFTN
                     AVTLNTTLSDYNENNGVEHNHTYSSESGYSDVNAQERKILSELVSSSESVSSSESVSN
                     SESISTSESVSNSESISSSESVSSSESISTSESVSTSESISSSESVSSSESVSSSESI
                     SSSESVSNSESISSSESVSNSESISSSESVSSSESISNSESISSSESVSTSESISSSE
                     SVSNSESISSSESVSSSESISNSESISSSESVSTSESISSSESVSNSESISTSESVST
                     SESISSSESVSSSESISSSESVSNSESISNSESVSSSESVSNSESISSSESVSNSESI
                     STSESVSTSESISSSESVSNSESISSSESVSNSESISSSESVSNSESISSSESVSTSE
                     SISSSESVSSSESVSSSESISTSESVSNSESISSSKSVSNSESISSSESVSNSESISS
                     SESVSNSESISSSESVSSSESISSSESVSSSESVSTSESISSSESVSNSESISSSESV
                     STSESISSSESVSNSESISSSESVSSSESVSSSESVSSSESISSSESVSNSESISSSK
                     SVSNSESISSSESVSNSESISSSESVSMSTTESLSESEVSGDSEISSSTESSSQSESM
                     NHTEIKSDSESQHEVKHQVLPETGDNSASALGLLGAGLLLGATKSRKKKKD"
BASE COUNT         4166 a         2385 c         1837 g         4388 t
ORIGIN      
        1 agaaatcaaa tagtataatc atttaataac ctcctcaaaa aaaaaaactc caattttcta
       61 taattttatc atttaaaaat ctactagcta ttctataaga atactcgtgt tttttacaaa
      121 tatctttaga attaaataaa gcaacaatat actgagcaaa agcagttgat atttgttgag
      181 gattatctgg tgtttccctt ggaataaggt agccattcaa attattttca ataaaggtct
      241 gattaccata cggaacatct aaaccaatta tcggaagccc cgatccgatt gcctccatca
      301 aggttaaacc aaaaccttca ctggtagaac ccgataaata aacttgataa tctttataaa
      361 cagaagatag gtttttatgc cccattaatc gtatataatt atttgcctta tttttagcaa
      421 ttatttcttg taacttttgc cgttcgccac cttcaccata aatatcaaag gttaattcag
      481 gaattacttt attagcttct accacagcat taattaacca atcaatatgt ttttccgaag
      541 ctagacgtga gcaagttaac aacgaaaaag cttttctttc atgccactct tttctctgta
      601 attttgataa acttcctaca ggaattgtat aaacgaccgg ttcaatattt aaatactttt
      661 taaattgatc aatcaacgtc ttagtttgcg tttctgttga ggtgataaaa gcatctatgc
      721 tatcagcatt tgaaaattga tagtcataat aattattcca taaaatcgta gtttcatcag
      781 ttgcgctaac attataatgc tctgcatgaa caacaacagc tactttagca tttcctttat
      841 tccttagaac agcttgccca atgcctgtag agcggtctaa caagattaga tcactattcg
      901 ttaattgtaa tttttctaac atgtatccaa ctaattcttc tttagaatat agtatagttt
      961 ctttgaattt aaaaatacta ttctcgccat ctacattttc ttcatatgca acacttccat
     1021 cttcattata aaatgtacgc tgatacaggc atggacctat atcttcggga gcataatatt
     1081 ctgaaaacat tttagtatat gagtagtagt cttttctaac aagctttccc cccataagat
     1141 actctaccct ttggacgatg ttactagatt catcgttaag cataactgta actacaatac
     1201 tacttccttt aaaaaaaaat gatattattt taccgttttt ctcagtccta tcaatcggta
     1261 gtgaaaaact ttctttcaat tgttgcagtg aataagaagt tgcagcaatt gttaaatcag
     1321 taaaaaaagt ataaagccat ataatctcat tgtcttcaaa gccgatattt cgagtaaggt
     1381 gctctatatt ttcattctga aacatatcag taaaaataaa tttagcttca acacctaact
     1441 ttctaaaaac ttctgaacga tatgcttgag catactctac accgctactt gcccaaccta
     1501 tccctctatt caaattataa actaccatta actccgtttt cctttataca taatatattg
     1561 agtaacttcc atcacttgct tttgaaattg tacttaacat aatattctta acgatagaat
     1621 atcttatttc ttttcccatc ttatagtaag aattaagcga ttctttataa tattcttgaa
     1681 taatatctcg ttgaccgata cctctattgg ttacaactcc ctttaattgc tgtaggctat
     1741 ccacttcttc tatccacgat ttgtcaatag cctttaatat cgatattctg acgaaattat
     1801 caaattcatt atcactttga agtttagttt tctttgcagt aaattctcta tcaaatatgt
     1861 caatcaataa tttaaataca tcgtcagaat tatgaacgtc aaattcatca gggaaatatt
     1921 taaatttata ggtaagattt tctaatatat atcttcgtaa agaatgttcc gttaaatcct
     1981 tatcattagc aatatcgtta aaattatcct gaataatttt tatcacacca tcaaccacaa
     2041 tggaactatc agctattaat tcatttcgta attgataaat attttcacgc tgtatacgca
     2101 agctctctcc gaacttaata gtttgctccc tagaagatac acctttatct tcacttctga
     2161 gttgagcatt tttaactatt tgataaaaaa agcgtttctt tctaggtacc atatagtaat
     2221 ttgaagtttc tttacgagaa tattttttta aatactttat cacccattcc ggagaatgag
     2281 aaacaattaa ttcatcctct agcgacacat aaaattggct aatccctggg tctccttgac
     2341 gccccgaacg gcccctaagc tgccagtcca tacgtaaatt aggcatcctt tccgttccta
     2401 taactgcaag tcctcctaat tctctaaccc cttcacctag tttaatatct gttccacgac
     2461 cagcaagaac tgttgcacat gttactgaac ctttttgtcc cgcttctttt ataatttgtg
     2521 cctctttaac aacattatca gctgttaagg tattatgggc aatgccttct cgcagcaata
     2581 actttgaata aatgttagca atttcaactg ttccagaaat taacaatact ggctgacctt
     2641 tactatgtct ttgtttaaca tcatctatag tagcttgcaa cttttctggt aagctaacat
     2701 atattttatc ggcaagatct actcgtttga tcggaaaatt tgttggtata acaataacag
     2761 gcaggtggta agtttttata agttcatctt cagaagattt tccagttcct gtcatcccag
     2821 acaaccttgg gaacatatta aagagacttt gatacgttat tgaagcaatt gccctagatt
     2881 catttgtcaa agcaacatct tcttttgctt caatagcttg gtgaatccct gattgaagtt
     2941 ttgtgccttc cataacacga cctgttgcgt tatctaacaa ttttacttca ttatttttaa
     3001 ctacataatc ataatctctt ttatacaaac aatgagcacg caatgctagg tttatatgtc
     3061 tatttaattc ccaatactca tcattatata attctgaaat attgaagtac tgttttgcgt
     3121 aacttacacc cttctcagta agatagacaa ttttatcctt agctttatgg atatattcat
     3181 cattttcctt aagagtgagt acaaaattat tagtgatacc atagagatta gattgtacac
     3241 gaggatctcc cgctataatt aagggtgttt gagcaatatc taacaaaaca gagtctgctt
     3301 catcaacgat aacaaaatga aaaggtctta gaaattgttc tagtttatta ctagccagat
     3361 tgtgaacgag atagtcgaaa cctaaggcac ctgcagtagt ataagtaata tcagattcat
     3421 atacttctct cttaatagtc gcatctgctt cttctttttt atcaaaaaca ttaataccaa
     3481 ctgtcatacc tagaaattga tatacttttc ccatctctaa tgcatctcgt tttgccaaat
     3541 aatcattggt cgtcactaag attgctccct tgctagttaa agcattcaga tacaagggca
     3601 tagttgcagt taatgttttt ccttctcccg ttttcatttc agctatcgtt ccttgatgaa
     3661 gagcaatagc acccataacc tgagcatcat atggaaacaa acctaaaatt cttttattag
     3721 cctctcttat cacagcaaaa gcatcaacaa gaatatcatc tagactttct ccacttgaaa
     3781 gtctattttt taatattaaa gtttgatggc ttaactcctt atctgataat tgagcgtagt
     3841 acactttttt attatttatc tcgtttacaa ttttttgaat tctttgcaat ctcaatttat
     3901 ttagaataga gattttgggt ttaacaacaa cttttttcat tttttatcct tagaaaatgc
     3961 tatcaacgtt ctaataagtt caagctctga tggtaagctg ctttcaacat aactgtttag
     4021 ttcaatacca tctttttctt cttgagtctc aattacttca aataattcta ggtaacaaaa
     4081 ttctacactt atacaacccg caccaataag agaaatatca taaaaatatg tgtcatcagg
     4141 aactataaaa tctgaggagt gatctcgaat aatagaaaaa cctatttttt ctttttgacg
     4201 attataaaaa tttatctgaa taatcacgca tccctcaggc acagtagtta attttgatac
     4261 caactcatag gtatgattag gttttagtaa ggtaagctct ggcaacgttc tattagcttg
     4321 aaaattcgtt ttagaaagcc attttttgat tgtttttcct gtagagaatc ttagattctc
     4381 aaaaaaaaca cgattatgct ccaaatactt tatagtcgaa ccataaagat aaacattgct
     4441 actatcagtc caaagcacct tctgtattct ttttttaatc actaaacact ctcccaaaat
     4501 catcactgag aattgtttta tactgagaag taaaccaatt atttattgat tgactattat
     4561 cattatgtcg cccagcatat ccacttgcat acaacagggt gttagtttta gaaaaataat
     4621 caatcagcct tggagttgca tttctatcat aatcatcatg tttcatataa gatatagcaa
     4681 actgcgtttt ttcaaaatta gaacgcttaa acttattcca aaatttttga ttaaattgat
     4741 taatagcttc atcgttttga gaaccaataa tattttggag aatatccata ctcgtttcaa
     4801 aatcatctgg tcttcttaat tttaaattta ctaccatatc gcctaaattt gtaaagggtt
     4861 tccccacaac tatagcatgg ggggaaaaag tagaaccata ataaagggca ccaaaacttc
     4921 ccatagataa acctgataaa ataagttgac aatgatcaaa ttgtaggtaa tctaaatatt
     4981 tttgtaaaac attttctaat tcactttcgt attctgaagt acctaggtag aagcttccac
     5041 cttcaatcct tggatcacca attaagagaa acggagctcc caaacgtttc atcatgtaaa
     5101 aaccctcaaa accttcggct cctcgaaaac ctgaaaaata aacattcaat ggaggtttca
     5161 tatctccagg gttaaaatag tagataaact cttgcctttt ggaatcactg tacctttctc
     5221 caccgattaa aaattgaccc aaacctttac gcgaatatcg ccaatgtagt gcaccaaatc
     5281 tcaccactcc gtcacctcta gcaaagatag aaattgatga aaaaccaact gattcatcat
     5341 attctaaaat atatggatca gccaaatcat tttctgaaag gtattttgta tttaatatca
     5401 aatcaagtga tcctttttgg tattgaatga cttctatcat aatactatta tcaccttgca
     5461 atttttgata ttcttgccat atttctatcg ctttatcagt agaggataaa ttatagcgaa
     5521 atgagaatag aggttgaaaa tcttctccaa aattccctga aaaacaaata gagctattgc
     5581 cttcatatat tatctctccc ttaaaatcag gatttagatc tatgtcagaa accttcaatt
     5641 ttgcaccata ttgttccgaa aaaagagtac ggcttaggaa aataattttt tctttccttg
     5701 atccattaaa tggtagtggt ctcaaaacct tacgtttaaa tataccgttt tctgagcaaa
     5761 aatcgagttg aaaatcctcg tcgtaaaaga gtgtatgcgc ttctaagcaa tctgaaagta
     5821 ggttcaattg caattcgcta acctcactag ttaaaatgac cgcattaaac cataatttaa
     5881 cttcattagt tgaagtttta ccaatatatt caacattatt tttttctaac tctttacttt
     5941 ttaatttctc taagaaaatt tgaatagcat ttccattaca gtataaccat tcaatattgt
     6001 ctggagttat taaatcagca tcccatggtt cctcaccaat ctgcaaaatt tttaattttt
     6061 ccatattatt ctttttctaa taattttcgc cagcgctgta atatcttacc gcttgtataa
     6121 tcacccattt tttttacgct gtaaactaac gatttattcc aattcattag tccatcaaaa
     6181 taatagtcaa ttgcattatt gagttctgta gagtcactaa taatccagcc attcttctgg
     6241 tgttctacat aattagtgtg aactttattg atttgaggaa ttccagaact cagagcagca
     6301 atttgagtat ataggtctgg atcatctctt aaatcaataa ctaacctagc agtatctaga
     6361 gcatctataa tttgattttc atttgtatat atatcaaaac taatgccacg aattttagtt
     6421 ttttgttctt ccttaaaatg attctcatct tccaaagaaa gagaggtaaa aaaataatct
     6481 ggatttttag aagacaatat tttgtcctta acttgttctt ttagagaagt catatcataa
     6541 tcatttttat agctgactat tttcaactct aatgacggat taacttccat ttttgctaat
     6601 aattggtcta aaacttcact taaataagtt tgagaaagat catctacaag taaatatgca
     6661 ataagctctt caatattttg gctctgtccg agtctcaaac gagtatcaaa agggggaata
     6721 cggctaatgt gattctcaac atagtttgac gaataatttt tctcttgtaa tgaagtaatc
     6781 aataaccgtt ctattttttc gctttcagca ataaacaatt caccttcatc aagaagttca
     6841 ttaaagtcga gtcgattatc aaatgaatag cgttcaccaa aaaatgagaa aattttcttt
     6901 tgatctggaa atgattttct gactaaattg ttatgaatat cgtttactgc tactataata
     6961 taatcatctg gagtcacttc tagttttttt aaaaccaata ttctctctcg aataagagta
     7021 tcccaatcat catactcagt aaaattaaac accttatcag acattggatt aactactatt
     7081 ctcggaaatt ttgaagcata ctctcgaatt tgccaaacac cattttgatt taaataatct
     7141 tgataaatta atctattttg tttgaaatat ttgatactgg atacaaaacc acgatcgtca
     7201 aatatcataa tcttatcaag cacaccatct attaaaagtt caatcagtag taaattccca
     7261 ttttccgcaa attggatacg tgcaataatc tcgccaccat gtttagctaa tactaaaaaa
     7321 ggtgtataaa caaaataagt ccctttaggc caattcagtt ctttaaaatt aattaactta
     7381 atctcttttt gttgaatatt ttgaatatcg tcaaaaaaag accaataaga gacagccaac
     7441 atactctctt catgaagcat gtatctcaac tgaggattat aactaaatag aagtaaacgc
     7501 acttcctcat ctgcattctg aaacattcta acctgactaa cagtatcatc aaaagtcatg
     7561 ttctccaata cacgatacca gataggagct tcattatgcc acttttgatc agaattgtac
     7621 cacgaaggaa taaaataaaa catgattgct ccttaaataa tatttcggtg actgccccac
     7681 aacttcaagg tattaacttg ctcaataata ctgagcataa aagttgaaat aatataaata
     7741 ttaatgacga tcattgcaat actaacttgg tttgtcttcc ccaccacagc caacattggg
     7801 agacctccca taataaccgt aaaaattgct ccaactactg ccatgatact aattttttct
     7861 tgtaaaaaac gttgagtagc agctcctgga gttacatttt gaaaatattc cccccgttga
     7921 cgcatatttt ttgcaatatt atacgggtcg taattaaaat atgcaaatcc aatgctcaaa
     7981 ataaataata atataacaaa aagtccaata cctaaaggct tattaattgc aatcattgac
     8041 gagatctgaa tcaataatcg ctcgtttgga aaagcaagta acaatgcttg aattataaac
     8101 gttggaagca tcattaatgt catcccatac atgaaagggt aggaccctgc tggatttatg
     8161 cgaataggaa tataggtatc ttgggcaaat ttactaacga tatcaatacg cctaataggt
     8221 atacgatatt ctgccctata aaccaccgtt gagattaata ataaaacact aagtattata
     8281 cttatagcta taatagttcc cagcagatcc ataatactaa aatgtgactg ttcaaaataa
     8341 gttattattc cagtagtcgt tgcttggagc atgctcacta atatgataac aatacttcct
     8401 cctagccctt tagaagcatt gagattagca agccacatta acacaaatga gccacttatt
     8461 aacaagacca ttgtcaatgc tttaaaaaca atatcactat aagaacctaa taaagagctt
     8521 tttataaatt gatttcctga aacaccatat gcttgtataa ctgagatagt aagcattaaa
     8581 gtatattgag ctacttctgc ttttcgcttc gttgcatttt ttgcccacga aaataaggaa
     8641 acgaatctcc aaataatcat acttgacatc catggactca accccagtgt gaaaacattt
     8701 aatttattaa aactcccacc acttactgct gaaaagttat tgagtaaatg ttgatctact
     8761 gctagtttta tctcttgatg tgataaagga acagtggata ataaaacacg ttgtcctaac
     8821 atatatacac caacaattaa aatagtccat aaaatacttc ttaatagcgt tgactttcta
     8881 tagctataat ataattttcc aatcattttt tcccttttca aatcaattta tcacttaaga
     8941 cagtataact acttactatc ttaagtgata aatcgaaagt actttcgatt tatcatctaa
     9001 atatcacttt gaaaattaat cttttttctt cttgcgagat ttagtagctc ctaacaacaa
     9061 tcctgcacct aacagaccta atgctgaagc cgagttatca cctgtttctg gtaatacttg
     9121 atgcttaact tcgtgttgag attcggaatc ggatttaatt tcagtatgat tcatcgattc
     9181 agattgactt gatgattctg tacttgagct aatttcagaa tcccctgata cttctgattc
     9241 acttagagac tctgtagtac tcattgatac tgactctgaa cttgaaatag actctgaatt
     9301 tgataccgat tctgaacttg aaatagattc agaattagat accgatttgg aacttgaaat
     9361 agactcggag ttagataccg attcggaact tgaaatagat tccgagcttg atactgactc
     9421 tgaacttgat actgactctg aacttgatac tgactcggaa cttgaaatag actctgaatt
     9481 tgataccgat tctgaacttg aaatagactc tgaagttgat accgattcgg aacttgaaat
     9541 agactcggag ttagataccg attcggaact tgaaatagac tctgaagttg atactgactc
     9601 ggagctagat accgattcgg aacttgaaat agattctgag cttgatactg actcggaact
     9661 tgaaatagac tcggagtttg ataccgattc ggaacttgaa atagactctg aatttgatac
     9721 cgattcggaa cttgaaatag attcagaatt agataccgat ttggaacttg aaatagactc
     9781 ggagttagat accgattctg aagttgaaat agattccgag cttgatactg actctgaact
     9841 tgatactgac tctgaacttg aaatagactc tgaagttgat accgattcgg aacttgaaat
     9901 agactctgaa tttgataccg attctgaact tgaaatagac tcggagttag ataccgattc
     9961 ggaacttgaa atagactctg aatttgatac cgattcagaa cttgaaatag attccgaggt
    10021 agatactgac tcggaagttg aaatggattc ggagttagat accgattcgg aacttgaaat
    10081 agactctgaa tttgatactg actcggagct agataccgac tcggagtttg aaatagactc
    10141 ggagtttgat accgattcgg aacttgaaat agattctgag cttgataccg attcagaact
    10201 tgaaatagat tccgaggtag atactgactc ggaagttgaa atggattcgg agttagatac
    10261 cgattcggaa cttgaaatag actctgaagt tgatactgac tcggagcttg aaatagactc
    10321 tgaattcgaa atagattctg agcttgatac cgattcagaa cttgaaatgg attcggagtt
    10381 agataccgat tcggagcttg aaatagactc tgaagttgat actgactcgg agcttgaaat
    10441 agactctgaa ttcgaaatag attctgagct tgataccgat tcagaacttg aaatggattc
    10501 ggagttagat accgattcgg agcttgaaat agactcggag ttagataccg attcggaact
    10561 tgaaatagat tctgagcttg ataccgattc tgaacttgat actgactcgg agcttgaaat
    10621 agactctgaa gttgataccg attcagaagt tgaaatagat tctgagcttg ataccgattc
    10681 ggaacttgaa atagactcgg agttagatac cgattcagaa gttgaaatag attcggaatt
    10741 agataccgat tctgaacttg atactgattc tgagctactt actaattcgg ataaaatttt
    10801 tctttcttga gcatttacat ctgaatatcc cgattcagat gagtaagtat gattatgttc
    10861 tacaccattg ttctcattat aatctgataa agtagtattc aaagttactg cattagtaaa
    10921 tggtgtttct acaacaatta catacgtttc atttgatgca ataaactgcc cccaataaat
    10981 atcataccac cctttttctg ggtaactatt tgcaaactta ctagttacat cctccatgct
    11041 tgaataatcc tgtaccatac tctctacaat attatccgag gtggttttat agatttttat
    11101 cgttgaattc tttaaatctg ctgtactctc tttgctataa ttatatatcc tagtatataa
    11161 cccgttgtaa ttatagagtg attttccatc tacattaatt tgacttatat aagtcatctt
    11221 ttttcccaca tcatcaatgt tggttattcg agaatctaca cttactcccc ctgcaaaatc
    11281 aattcttacc ggtggatcat atttcactgt ttctttaatt gtagcctctt gatcctttat
    11341 ttttaccgaa aaaagaacat cttcttttgt gttttgaact gtcgtatcat ctggccataa
    11401 tggaatatct aatattccat taatattatt ttttgaagcc gcaaaatctg taaaactata
    11461 gactatagat tttttttgag aatcataaat tccagtcgca tagacctcac ccgtaggtga
    11521 ttttataggt tctacaatag aatagttaga agctccattt gtatcgatat tatctgataa
    11581 cttaatttca aaagtatcac ctctattcac agaatcaata gtgaatttta aagacatgtg
    11641 catactttct gacttatcca tatttataac acctgctttt gcttgcactc ctccagagag
    11701 ctttatatct aaattttgta acttatctgt tacatcttgt ccttgaacag ttgttacatc
    11761 tccttcacca gagattaatg ctgctggtaa agtttccata tctgaagcac tagatacaac
    11821 tctcatcgct ctcggttgaa ctctagcggt cgttgccgct tctgatgatt gaactgattc
    11881 tgatgctgag acactctcac tactagatag agataaagaa ctatattctg ataacgagct
    11941 agactcagat aaagaagcag aggtagaatc cattagtgat tcactggcag ataatgaggc
    12001 gctggtagat tgcaaggtac tagttgacat tgattgtgat tcacttgttg aatgtgatga
    12061 agaatctgag tttacagttt ttacttctgg cgcaaccact acagtttcag cagtagctaa
    12121 tgttttttca tttcctgtaa gttctgttgc agtaggtgtt tcttcagctg agacagaagt
    12181 tgtgtctgtc gtccctccaa ctacagctcc aagagctgcg actgctgtta ggatgctagc
    12241 agacgtttta gttctgctta aatcttcact agtagtttga accttagagc ttttctcttt
    12301 accgctaaac ctcgaaagga taacatagcc aatttgtgtc ataacacttt tgacccagtg
    12361 cttacctgat ttcctcattt taactcgaac tttgcgtgat ttttcaccaa agtttccaaa
    12421 ctgctttttt aacatttttc ctccatattt aaaaaataat gctatataaa aaacttttct
    12481 aattctaaga attctcaaat atagctataa caaaatacta ctttgttatt tcaagttcta
    12541 aaatattgct tgaaacatca tcaataaata gcatatttgc ctataaagac ccctcctatc
    12601 ttaaaaagct acaataaaca attcttcctc tcttctgaaa attaaatcaa ctattgcttc
    12661 tttctaatct aaataaggtg tttttaaaaa cacttttcgt attataacat actttaatca
    12721 aattggatat aaaatttcag tttttttaac acttattttt tactactatt attcaa
//
LOCUS       DATROD010000042        12733 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.042, whole genome shotgun sequence.
ACCESSION   DATROD010000042 DATROD010000000
VERSION     DATROD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12733
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.042"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(937..2094)
                     /locus_tag="VBC74_001134"
     CDS             complement(937..2094)
                     /locus_tag="VBC74_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbhA"
                     /protein_id="HEO6942089.1"
                     /translation="MKKRIIVVLVALLAIGGGSAYYFLSYAPHQEAVTNFNKAVQTVK
                     SKNKTLKSEIAKAEKLVKSNQQPLDVRTLDNLKAAVATAKSDLREIPKVADKTEAIKK
                     QTKSLNQPIDYSKATQSLADSSLAYTNSVKQLAQITNPSQSFVEERLKEIDTVTGVQS
                     VTESNDPNGKLNKQGGYTASIYFTDNQVTEAVEGKDVLDKGTDAGGNIEVFKTKEEAE
                     KRNIYLSAFDSAGMFNPGSHYVFGTVVIRTSHYLTASQQKSLTQKIYEKLIEIKSKNS
                     GSTKDSKKKEVSKDNTKTSTQEQPAPSSTTDKSQESQSSSETSNSGGRGGTRGVEDSS
                     DTSSSSEMTEEELNSAIKEIQDSRDYYAPNEFENQVGDTTSSDNGDSTYHP"
     gene            complement(2349..2936)
                     /locus_tag="VBC74_001135"
     CDS             complement(2349..2936)
                     /locus_tag="VBC74_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6942090.1"
                     /translation="MNRLKELRANQKKTQQDMSEFLNMSRRGYQKIENGESQIKPDKA
                     QALADYFNVSVGYLLGYSDDKKDPMLTSFELARKDDTGEYFDLQDMVTLMDISMLKNV
                     DTRDKILSNLKEYYDYYGIRMKEEHEIKDSEKFNAFLAEFQEDTNDYIIMLLTGIVKL
                     RDDIRFTLLDILAMEGEKLETVKNVVKFISDNGDN"
     gene            3091..3276
                     /locus_tag="VBC74_001136"
     CDS             3091..3276
                     /locus_tag="VBC74_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942091.1"
                     /translation="MLITSTQAKAIRRKQADKQLTSKSASAEIGVTEVTYRKVRNGGE
                     VKNGIYQKVMEWLAEDY"
     gene            3292..3894
                     /locus_tag="VBC74_001137"
     CDS             3292..3894
                     /locus_tag="VBC74_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HEO6942092.1"
                     /translation="MRTETWNGYDIRFVEHQGEWWAVLADICHALDLKPKRVKERLVD
                     EVVSTDHVADSLGRQQEMLIVNEFGIYDTIFSSRKPEAKSFKFWVFETIKQLRQATGL
                     EGFEVFRMLDKEHQKEAMARLTNSLDRVSKKDLIKANTITNKAVSNKFGYSKMVKKSE
                     MTPDMLVAREMILDDTVELMGIKEKFGLNISVSESIYNKN"
     gene            4130..4768
                     /locus_tag="VBC74_001138"
     CDS             4130..4768
                     /locus_tag="VBC74_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HEO6942093.1"
                     /translation="MNIIKQVKSSFGELEIDFYLDRNRNIFVTIEQLAQGFGYKSRNA
                     IEKMIERQPYLKEKRFSVTDKLSATDGKQYETRLFNKRGIFEIGMLSKTEKGKIFRQW
                     IYDHIEELERENANFKLIRELEKSNHKELTQAIKDWEHFNQWSYKAISDLLLKSVTGQ
                     TAKQLKQSRVGYEIALDCLSADELTRYRKLEQKVIVLLELNAEYNDIKKLVL"
     gene            4867..5040
                     /locus_tag="VBC74_001139"
     CDS             4867..5040
                     /locus_tag="VBC74_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEO6942094.1"
                     /translation="MTDKRAVGRPPKGEQPNNIKITVRISQGLDEKVQAYADNHGLTK
                     PEAIRKALEDLSD"
     gene            5129..5635
                     /locus_tag="VBC74_001140"
     CDS             5129..5635
                     /locus_tag="VBC74_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942095.1"
                     /translation="MRTLFQSTQKTERECAFCVLKNQNNMIKKKRNPRVLFKNVAYKL
                     SEIEGKTLTEIASFLGFGNSEVCRSTLYNWKRRKWLTFDLKNGHYRNVEVLHEVTLEK
                     MANKELKEQGLIYKASIYYEQVVSTSEIIEDIKTKTQDRIKAIHLQQKALERIPSELF
                     AELYTNMN"
     gene            5712..6044
                     /locus_tag="VBC74_001141"
     CDS             5712..6044
                     /locus_tag="VBC74_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942096.1"
                     /translation="MKYRVETNPFSKDRYTPEQREMLKKRQLSKDKAEAYFARLYNHH
                     IARVIIANVMAEYTTTFRKSATTFEEAWGALGYKQTTEIVFRAVNGLPCSEKDTGELE
                     TYLSEVSA"
     gene            6041..6262
                     /locus_tag="VBC74_001142"
     CDS             6041..6262
                     /locus_tag="VBC74_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942097.1"
                     /translation="MRCIEELNEIANDILVDYEGLCGQLMDILNVLDLAAQEYSKETT
                     SAIINTSCRALKCLISDHDKMTHKYRKEL"
     gene            6265..6456
                     /locus_tag="VBC74_001143"
     CDS             6265..6456
                     /locus_tag="VBC74_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942098.1"
                     /translation="MQELNFTLTQTLILFIVLGLIGLLLSRSKPLIEIDLPEDTQAPK
                     PRQNANYGAYIQAQNHYYN"
     gene            6467..6795
                     /locus_tag="VBC74_001144"
                     /pseudo
     CDS             6467..6795
                     /locus_tag="VBC74_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6797..7069
                     /locus_tag="VBC74_001145"
     CDS             6797..7069
                     /locus_tag="VBC74_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942099.1"
                     /translation="MNVFSQDYEAKLLEQNLTAFNRFLEVYQQPKPRVLGLITAEQVK
                     EELNIKGKTLKRWENAGLKRYQPPLEDTRKHYYKVSDILIFLGVDV"
     gene            7070..7928
                     /locus_tag="VBC74_001146"
                     /pseudo
     CDS             7070..7928
                     /locus_tag="VBC74_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029301.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
     gene            7940..9334
                     /locus_tag="VBC74_001147"
     CDS             7940..9334
                     /locus_tag="VBC74_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
                     /protein_id="HEO6942100.1"
                     /translation="MKDYQNKLSQATQPAFAPAFRTRKGRGDKEYVISSPYNVGKVFE
                     FYENIFTGIKYNEFEKTIEITKTLPWSKEKGLWTNEQTSLCIAFIDDKYRFTPRKEHI
                     EVAITALAKKNSYHPIKQRIESQKWDGKARGERYFIDLLGCADNSYNREIAKVWLTGL
                     IARIYLRKVKFEVVPILIDKRQGTGKSTVTKRLLPSYHTDSEIKFGKNDSDYQKIQAN
                     AIIELGELKGMSKAEIETVKSFISSDSDTYREPYERKATPHPRHCVFIGTANKKSFLK
                     DSGTERRFFPIECGINDVEKHPMEVEEDYFLQILAEAKVWFNNYEPLTPSKELMNQLA
                     DIQEDYKVEDVDKEIIDQLLNEFQIVEGWDSLSQYEQRQYVLKQLGEPLDNAQSYSDY
                     PSAQTNCLLQVTSPNHIAYLGFNQKPTQGGKALISQKIRDHLDNDDGWKKGENPARKR
                     LFKGGTPVSYYERV"
     gene            9627..9884
                     /locus_tag="VBC74_001148"
     CDS             9627..9884
                     /locus_tag="VBC74_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942101.1"
                     /translation="MNPNYTNHGIKKMHYHYDDLPNDQLYRLELPNGQCGYDFISKIN
                     FNTKSKPGDCDFTRPELIRRGYRWVFDCGFAIPVEKGEQTQ"
     gene            9881..10054
                     /locus_tag="VBC74_001149"
     CDS             9881..10054
                     /locus_tag="VBC74_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2758 domain-containing protein"
                     /protein_id="HEO6942102.1"
                     /translation="MKIKLFYQKHKESLDDFEYRINQFTLSVSVIDIKFSEATYGNYE
                     DMSNTTSLLVLYR"
     gene            10212..10769
                     /locus_tag="VBC74_001150"
     CDS             10212..10769
                     /locus_tag="VBC74_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942103.1"
                     /translation="MRTFSDTPKTFTFHYTFKDFDTAQVACHAILGYMTGSYEQPVID
                     ATYHNDDQGGHANQLVLEYAEDRKLNKVFKRICDSFKDYYNQPEDMTDEELDDVAQET
                     ELLKEVESTDKKRVVPLFDNSQEKADKQDIFMAFISDHNQLAEHVSMNYKKMTQEDLG
                     AVLESISQGFNYLYDMAIEGELLVK"
     gene            10842..11330
                     /locus_tag="VBC74_001151"
     CDS             10842..11330
                     /locus_tag="VBC74_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942104.1"
                     /translation="MLELAIESIIKPMKKQEKTRVTGTINDQSITIDLDNLIVHYEGQ
                     EFLLETIPGTYGGKRYFFLCPDCERRCRKLFKASHAFACGTCQKLHQATLNRSKTDCQ
                     YYWQLAFKECLKVDSKAKHKHGYYSYDDFPKRPKYMRMTKYLYHWKRFHYYMDKGDKY
                     WL"
     gene            11337..11489
                     /locus_tag="VBC74_001152"
                     /pseudo
     CDS             11337..11489
                     /locus_tag="VBC74_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889234.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
     gene            11464..11841
                     /locus_tag="VBC74_001153"
     CDS             11464..11841
                     /locus_tag="VBC74_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEO6942105.1"
                     /translation="MSKKNAIRKLKEFHRWQRIANSLDLSYNERYQFDIKYHPTRREH
                     LEISRECALEELDAIKYAINQLSKIDYRKILIECYLISEKKTQQDIIAELNKSQSWYY
                     ETKKRALLEFAQSYRNGVLITTA"
     gene            11910..12197
                     /locus_tag="VBC74_001154"
     CDS             11910..12197
                     /locus_tag="VBC74_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEO6942106.1"
                     /translation="MHALEKNTQVNFKTNSDLLEKAKAIIAAQNLDMTASFNLFLENI
                     VQNKALPFETDTDKERAELLAGLRAEIAKSFDDLEHGRVYNASEVRANLGI"
     gene            12187..12534
                     /locus_tag="VBC74_001155"
     CDS             12187..12534
                     /locus_tag="VBC74_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEO6942107.1"
                     /translation="MVSDNKTHSLIIPETVQEQLQEIKSYIETTYFSEQAGANTVNNI
                     LYGLERLEFFPEAGFNADDRVGETIYPPHNTRCIVLGDYLAFYHILEDRKAVFVSDII
                     HSKQDYIKLFKKK"
BASE COUNT         4412 a         2275 c         2385 g         3661 t
ORIGIN      
        1 atattataaa tagaaaattg tgatataata ttgttgtttt ttaagacctt gcgagcgtgg
       61 aaaacttgcg aggtcttttt ttgtttggtt ttagtaagtt ggtaaatttt tagatagccc
      121 agaaaagctg gctgtctctt ggctattgtt agccgtatat atcaaaaatt gtaattcatt
      181 catggctatt cctttaaggt tacccccatc attaaaaaat gttgttcaat ccgagtttgg
      241 aaaccggtgg gaagggtcaa ctgtccaaat attcgctttt tttctcacac gacccccctc
      301 cccagggaga aaaactagaa aggagatgtg aatttttgga tgaattaaaa cgtttagaat
      361 agcaatctca tcacatttat aagcaaaatt aacacagaca gtagcaaaag aacttccaaa
      421 ttattcaacg ttgagtaatt taggtagttc agccctctac ctcatcgcta cccttccaga
      481 tgaccaaaag caagaacagc ttgaataacc tatggcttcc tatggtggaa ggctgacgct
      541 cctgaatcgt tcaacgttga gagatttggc aactccgttg acatgttgct gtagtcccta
      601 aatgttggta cgtgccaaca taatacgtca acctatttca atagtcctaa aacgctcggt
      661 aaattcactt aaagaactta aaaaaacttg atatatcggt cgtttattaa tttatttttt
      721 ccgttgtgat agcttctaag atcagtagac ttttttagca tatagtattc agaaatcgga
      781 cttttcggct ctcctaaaag gttgttaaag ataattattt ggtgaaaaat gatggaattt
      841 cgagtgtgaa cagagagcga actccgagaa attggcttga agctagagcg aagtaagaga
      901 aatatagtaa acggtgtttc atattgaggg aataactcat ggatgataag tactatctcc
      961 attatcactg ctagttgtgt caccaacctg attttcaaat tcattcggtg catagtaatc
     1021 tcttgagtct tggatttcct taattgcaga atttaattcc tcttccgtca tttcacttga
     1081 tgaagatgta tcacttgaat cttccacacc ccgagtgcca cctctaccac cactatttga
     1141 agtttcactg cttgactgtg attcctgact tttgtccgta gttgatgaag gtgcaggctg
     1201 ttcttgtgta cttgtttttg tattatcttt gctaacttct ttcttcttgg agtctttggt
     1261 agaaccagaa ttttttgatt taatttctat caatttttca tatatttttt gtgttaaaga
     1321 tttctgttgg cttgctgtta gatagtgaga tgtcctaata acaacagttc caaatacata
     1381 gtgagaacct ggattaaaca tacctgcact gtcaaatgca ctcaggtata tattacgttt
     1441 ttctgcttct tctttggttt taaaaacctc aatatttcct cctgcatcgg tccccttatc
     1501 gagcacatcc ttaccttcta cagcttcagt tacctggtta tcagtaaagt agattgaggc
     1561 agtataacca ccttgtttat ttagttttcc attagggtca ttgctttctg tcactgactg
     1621 aacgcctgta actgtatcaa tctcttttaa gcgctcctca acaaaacttt gactgggatt
     1681 ggttatttgg gctagttgct taacactatt tgtatatgct aaagaactat cagctaaaga
     1741 ctgagtagct tttgaataat ctattggttg attaagcgac ttggtttgtt tcttgatagc
     1801 ttcggtttta tctgctacct ttggaatttc tcttaagtca cttttggcag ttgctactgc
     1861 tgcttttagg ttgtctaaag tccttacatc tagcggctgc tggtttgact taacgagctt
     1921 ttcagctttg gcaatctcac tttttagcgt cttattcttt gacttaactg tctgaacagc
     1981 tttattaaag tttgtgacag cctcttggtg aggggcgtaa cttaggaaat aataagctga
     2041 accaccacca atagctagca atgcgaccag cacaacaata attctctttt tcatgtttgt
     2101 ttctcctcta tgcgcgtgag ttaatgcgga aaatcaatat ctaaacccgc tagtccctct
     2161 aaaatattta ttatgctttc cctttctttt ggcgttagta aagaaaaata aaaaataagc
     2221 tttttaaaaa catttggggt atccccaccg tcaataaatt caataaattt ctttgtataa
     2281 agcgtttaaa tacagatacc atggttttat ttatatagat gaaacccttg aaaatagtgg
     2341 cgcgtgggtt agttgtcacc attgtcagat ataaatttga caacattttt aacggtttct
     2401 agtttctcac cttccatagc tagaatatcc aataaggtga aacgaatgtc atcacgaagt
     2461 ttaactatac ccgtaagcag catgatgata tagtcgtttg tatcttcttg aaattctgct
     2521 aaaaaagcat tgaatttctc actgtctttt atttcgtgtt cttctttcat tcgaatacca
     2581 taataatcat aatattcttt taaatttgaa agtatcttat cgcgagtatc tacatttttc
     2641 aacatagata tatccataag agtaaccatg tcttgtaaat caaaatactc tcctgtatca
     2701 tcttttcgag ctaattcaaa gctggttagc attgggtctt ttttatcatc gctatacccc
     2761 aacagatacc ccacagacac gttaaaataa tccgctaggg cttgggcttt gtctggtttg
     2821 atttggcttt caccattctc tattttctga taccctcggc gactcatatt caaaaactca
     2881 gacatatcct gctgagtctt tttttgatta gctcttaatt ctttcaatct attcataatt
     2941 tattacctca agtcgattat atcataaaaa gaaaactaag ctaacttttt ttacactttt
     3001 tagaatcacc ttcttgacaa cgaattataa gttagctaaa ataaaacctg ttaactaaca
     3061 aaaagttagc aaagaaagga gttgaaatta ttgcttatta cctcaacaca agcaaaagct
     3121 atccgaagaa aacaagctga taaacaatta acttctaaat cagcaagcgc tgaaattgga
     3181 gttacagaag tgacttatcg caaagttcgt aatggcggag aagtaaaaaa tggcatttat
     3241 caaaaagtca tggaatggct tgctgaagat tactaaagaa aggacaattt attgagaaca
     3301 gaaacatgga atggatatga tatccgattt gtagagcacc aaggcgaatg gtgggcagtt
     3361 cttgctgata tttgtcacgc gctagactta aaaccaaaac gtgtgaaaga gcgtttagtt
     3421 gatgaggtcg tttcaaccga ccacgtcgca gacagtttag gacgtcaaca agaaatgcta
     3481 attgttaacg agtttggaat ttatgacact attttttcaa gtcgtaaacc agaagcaaaa
     3541 tcatttaagt tttgggtatt tgaaaccatt aaacagttaa gacaagcaac tggtctagaa
     3601 ggctttgagg tctttagaat gctggataag gaacatcaaa aagaagctat ggcaagattg
     3661 actaatagct tagatagggt atctaagaaa gacttgatta aagctaacac aatcacaaat
     3721 aaagccgttt ccaacaagtt tggatattca aaaatggtta aaaaatcaga aatgacacca
     3781 gatatgttag ttgctagaga aatgatttta gatgatactg tggaactcat gggaattaaa
     3841 gaaaagttcg gattgaatat cagtgtgtct gagtcaatct acaataaaaa ttaaaaaaag
     3901 cgcaaaaatg cgcgtgaaaa caacaaaaaa aggcttaccg agaccaatca gcagagcctt
     3961 taactagtat aactaaactc aattaataaa gcaggcaagc tattattaaa ggggttttag
     4021 taaaagattt gatacttcca ttgtatcata ctaccgcaga gcaggcaacg acttaacagt
     4081 tgcagttctc cccgacaaaa caacaataat caaataacga ggtaaaacag tgaatatcat
     4141 aaaacaagta aaaagttctt ttggagaact tgaaattgat ttttatctgg acaggaatag
     4201 aaatattttt gtgacgattg aacaattagc gcagggattt ggatataaga gccgaaatgc
     4261 tattgaaaag atgatagagc gccaacccta cctcaaagaa aagcgatttt cagttactga
     4321 caaattgtca gctactgatg gcaaacaata cgagacccga ctattcaata agcgaggaat
     4381 ttttgagatc ggtatgctgt ccaaaacgga gaaaggtaaa atctttcgtc aatggattta
     4441 tgaccatatc gaagaactag aaagagaaaa cgctaacttt aaactgatac gagagcttga
     4501 aaagtctaat cataaagaat taacacaagc tatcaaggat tgggaacact ttaatcaatg
     4561 gagctacaag gctattagcg accttttgct aaaatctgtc acaggacaga ctgctaaaca
     4621 actgaaacag tcacgggtag gttatgaaat tgcattagat tgcctaagtg ctgatgagtt
     4681 gacgcgatat agaaaacttg aacaaaaagt aattgtccta ttggagctca atgcagaata
     4741 taacgatatt aaaaaattag tcctttaaca actgaataga gtacgcaaaa aagtttgatt
     4801 taggggttga cttattaaga cgacttaatt ataataatta tgtcgtcaaa actaaggagg
     4861 tatacaatga ctgacaaaag agcagtcggc agaccaccaa agggcgagca accgaacaat
     4921 atcaagataa cggttagaat tagccaagga cttgatgaaa aggtacaagc atatgctgat
     4981 aatcatggct taacaaagcc agaagcaatc agaaaagccc ttgaagattt gtcagactaa
     5041 acaaaaaaat agcacagtaa ccgccaacgc caatttttgg ttactatgct atcgctcgac
     5101 ccacaaagtg agtacgtaaa tattatacat gcgtactcta tttcagtcaa cacaaaaaac
     5161 agaaagggag tgcgcttttt gtgtgctcaa aaatcaaaac aatatgatta agaaaaaaag
     5221 aaatccgaga gtactattta aaaatgtcgc ttataaacta tctgaaattg aagggaaaac
     5281 attaacagaa atcgcttctt ttcttggatt tgggaattca gaagtttgca gaagtaccct
     5341 atataattgg aagcgtagaa aatggctaac attcgacctt aaaaacggtc attatcgcaa
     5401 tgttgaagta ttacatgaag ttactcttga aaagatggct aacaaggaat taaaagaaca
     5461 aggactcatt tataaagcta gcatttacta tgaacaagtg gtttcaacat ccgaaattat
     5521 agaagacatt aaaaccaaaa cacaagatag aattaaagct atccacttac aacaaaaagc
     5581 gctagagcgt atccctagcg agttattcgc agaattatac actaacatga actaatcata
     5641 aaactagcat gaatctagta taagaaacaa gcaattacac agaaaataat aattaagcga
     5701 gaaaaaacat aatgaaatat agagtagaaa caaatccttt ttcaaaagat agatacactc
     5761 ctgaacaacg agaaatgctc aaaaaacgcc aactcagcaa agataaagcg gaagcctatt
     5821 ttgctcgact atataaccat catatcgcta gggtgattat tgctaatgta atggcagagt
     5881 atacgactac attcaggaaa agtgccacta cttttgaaga agcatggggc gctttaggtt
     5941 ataaacaaac cacagagatt gtctttagag ccgttaacgg tttaccatgt tcagagaaag
     6001 acacagggga attagaaact tatttaagtg aggtatccgc atgaggtgta ttgaagaatt
     6061 aaatgaaatc gctaacgaca ttttagtaga ttatgaaggt ctttgcggtc aacttatgga
     6121 tattctaaat gtcctagatt tggctgctca agaatatagt aaggaaacaa ctagtgcaat
     6181 tataaacacg agttgtcgcg ctttgaagtg ccttatttct gaccatgata agatgacaca
     6241 taaatacaga aaggagttgt aaccatgcaa gaacttaact ttacactaac acagacactt
     6301 attttattca ttgttttagg tctcataggg cttcttctaa gccgttctaa gccattaata
     6361 gagattgact taccagaaga tacccaagca cctaaaccac gtcagaatgc aaactatggg
     6421 gcttatattc aagcacagaa ccattattac aattaggggg aactgaatga cattgcctga
     6481 aaattataga cgtgtcctta atctgattaa ggttggggca gacaatccca ttacagggca
     6541 gagattggct taatactgaa acgcgaagaa cgctccgtcc aaagtatcat cagtagctta
     6601 atcacgcgct acaacgtccc tattattggc attagacacg gattcaatcg tggttacttt
     6661 atcccagcta ataaagaaga attactagat ggtgctaaag ccttttataa tcaagtacaa
     6721 aaggaacaac aacgcctaag tgtcttattg aatgccgatt taaccagtta taaggaatta
     6781 ctcaaaggag gttagtatga acgtatttag tcaagattat gaagcaaaac tcttagaaca
     6841 aaatctgacc gcttttaatc gcttcttaga agtataccag caacctaaac caagagtttt
     6901 agggttgatc acggctgaac aggtcaaaga ggaattaaat atcaaaggta aaaccctaaa
     6961 gcggtgggaa aacgctgggt taaaacgata ccaaccacca ctagaagaca caaggaaaca
     7021 ttattacaag gtcagtgata ttcttatctt tttgggggtg gatgtgtaga tggctattta
     7081 tgaagcaaga ggctttagct cttatttgta cccctacaaa ggacaattag aaccgtttga
     7141 ctatatcgca cagtttaaac caatgaaaac gcctgaggct atcgatattg aagaatacaa
     7201 gcgaacacaa gctccgtact gtttgagtgg caaggtaacc ccagagaaaa acggtagcta
     7261 taagcgcaat aatgctagtc tagtctatcg tgatttgatt tttcttgact atgacgagat
     7321 agaaacagac gtaaacctgc ctaaaatcgt ttctaagaca ctttgggaat acagctatat
     7381 tattttatcc aacgattaaa cacacccctg agaagccacg ctatcgcctt gtcgtgaagc
     7441 ctagtgacac tatggacaaa acgacgtata aacaagtggt caaggagata gctaataaga
     7501 ttggactacc gtttgattta gctagtctta cctggtcgca attacaaggc ttacccgtca
     7561 caacaggcga cccagaagac tatcagcgct atgtgaaccg tggtcttgat tatcctgtcc
     7621 ctaaagcacc caaccgacag gttactacta cttacacgtc acgccctaga agtcagcgtt
     7681 ctattaccat gagggtcata gataccttgt ttaatggttt tggagacgaa ggcggacgta
     7741 atgtagcctt aactaaattt gttggtttgc tatttaataa atgggtggat tgtgatttag
     7801 agacggctta tgagctggta caaatagcta acagcgtgac aactaagcca ctacccattg
     7861 atgagataga caaaactttt agaagcatac tcgatacaga actaagaaag agaggaatca
     7921 agccatagaa aaggaagaat tgaaagatta tcaaaataaa ctatcacaag ccactcaacc
     7981 tgcttttgcc cctgcattca ggacgcgaaa aggtagagga gacaaagaat atgtcattag
     8041 tagcccctac aatgtcggta aggtttttga attctacgaa aatatcttca caggtattaa
     8101 atacaacgaa tttgaaaaaa ctattgaaat cactaaaaca ctcccttggt ctaaagaaaa
     8161 agggctatgg acgaatgaac agaccagcct ttgtattgca ttcattgatg ataaatatcg
     8221 gtttacccct cgtaaagaac atatagaggt agctattacc gctttagcta aaaagaactc
     8281 ttaccacccc attaaacagc gtattgaaag tcaaaaatgg gatggtaaag ctagaggaga
     8341 acgctacttt attgatctat taggctgtgc tgataattcc tataatagag aaattgccaa
     8401 agtatggcta acaggtctta tagctagaat ttatctccgt aaagtaaagt ttgaagtcgt
     8461 tcctattctc attgataaaa gacaaggaac tggaaaaagc actgttacta agcgcttgct
     8521 tcctagctac cacaccgatt cagaaatcaa gtttggtaaa aatgatagtg attatcagaa
     8581 gatacaagcc aatgccatca tcgagctagg ggagctaaaa ggtatgtcaa aggcagaaat
     8641 tgaaacggtt aaaagcttca tttcctcaga tagtgatact tatcgtgaac cttatgaacg
     8701 taaagccact cctcatccaa ggcactgtgt ctttatcggg acagctaaca aaaaatcttt
     8761 tcttaaggat agtggaacag aaagacgctt cttccctatt gaatgtggta tcaatgacgt
     8821 ggaaaaacat cctatggagg tggaagaaga ttatttctta cagatactcg ctgaagccaa
     8881 agtatggttt aacaattatg aaccactaac accatctaaa gagttgatga atcagttagc
     8941 tgatattcaa gaagattata aagttgaaga cgttgacaaa gaaatcattg accaattact
     9001 gaatgagttt caaattgttg aaggttggga tagtttatca cagtatgaac aacggcaata
     9061 cgtcctaaaa cagttgggag aacctttaga taatgctcaa agttatagtg actacccttc
     9121 tgcacagaca aattgcttac tccaggtcac aagtcctaac catattgctt atctggggtt
     9181 taaccaaaaa ccaacgcaag gcggtaaagc tcttatttct caaaaaatac gtgaccattt
     9241 agataatgat gacggctgga aaaaaggaga gaatcccgca agaaaaagac tatttaaagg
     9301 tggcactccc gtatcttact atgaacgagt ttaaaaacta cactaatact acataaaaac
     9361 tacacgtaat aacacaagcc aaaacattgg tattataagc tttatactct attgtagtat
     9421 tagtagtatt taatactata gataataata tttataacta atagtgcgcg tgcaaaaaaa
     9481 gaaagtcttt tggtcaaatt aatgctacta aaaacacaaa atgtcaaaaa cccttgatac
     9541 aactggcttt agccctgtgt tttttgctgt gctatttgat tttttaaatg ttaataacac
     9601 acattttaga gagaaaagag ggacatatga atcctaacta tacaaatcat ggcataaaga
     9661 aaatgcatta ccattatgat gacctgccaa acgaccagct ataccgattg gagcttccca
     9721 atggtcaatg cggttatgac ttcatcagca aaatcaattt caacaccaag tctaaacctg
     9781 gtgattgtga ttttacaaga ccagaactca tacgccgtgg ttatcgctgg gtctttgact
     9841 gtggctttgc tatacccgtt gagaaaggag aacaaacaca atgaaaatca agctatttta
     9901 tcaaaaacac aaagaatcac tggatgattt tgaatatcga atcaatcaat ttaccctatc
     9961 ggtatctgtg atagacatta aattttcaga agcaacttat ggcaattatg aagacatgag
    10021 taacacaact tctttattgg tcttgtacag gtaactaaaa ttacacacac gaggcggtaa
    10081 caccgtcact atacaaggag actgcaccct ttataatgag ttggtcaaat atcttctatc
    10141 tgggcaacaa ccaaactggg tagccatcat caatttatca gacattatag caatcacaaa
    10201 ggagaaataa catgagaaca ttttcagaca caccaaaaac atttacattc cactatactt
    10261 ttaaagactt tgatactgcg caagttgcct gtcatgccat tctagggtat atgactggga
    10321 gctatgagca accagtgatt gacgcaactt atcacaatga tgatcaaggt ggtcatgcta
    10381 accagttagt acttgaatat gccgaagaca gaaaactaaa taaggtcttc aagcgtatct
    10441 gtgacagctt taaggactat tacaaccaac ctgaggatat gacggatgaa gaacttgatg
    10501 acgtagctca agaaactgag ttactaaagg aggttgaaag tactgacaag aagcgcgtgg
    10561 ttcctttatt tgacaatagc caagagaaag cggacaaaca agacatattc atggctttca
    10621 tctcagacca caaccaactc gccgaacacg tttccatgaa ttataaaaag atgactcaag
    10681 aggatttagg agctgtactt gaatctatca gccaaggctt taattatttg tatgatatgg
    10741 ctattgaggg tgagttactg gttaaataaa caatcagagg gatttccctc tttttgtcgt
    10801 ttatcaatag ttttgggtta tttgaatact aaggagaaaa gatgttagaa ctagctattg
    10861 agagtatcat taaaccaatg aagaaacagg aaaagactag agttacagga acaataaatg
    10921 accaatctat caccatagac ctagataacc taatcgtcca ttatgagggg caagaattct
    10981 tacttgaaac gataccagga acttatggtg gtaagcgcta cttcttcctg tgtcctgact
    11041 gtgagagacg ttgtagaaag ttatttaagg cttctcatgc ctttgcttgt ggtacttgtc
    11101 agaagcttca tcaagcgaca ctcaatcgaa gtaagacaga ttgtcaatac tattggcaac
    11161 tagcctttaa agagtgtctg aaagtagatt caaaagcaaa acacaaacat ggttattata
    11221 gttatgatga ctttcctaag cgtccaaagt acatgagaat gactaaatac ttatatcatt
    11281 ggaagagatt ccattactat atggataagg gggacaaata ctggctataa cattcggaaa
    11341 aacagatagc tgttatcaaa gctagatacg tggatgactt atcatgggat gagatacccg
    11401 acaagctagg ttattcaagg aatactgcat tcaaattaca tagagaagct ttagaggtat
    11461 tagatgagca agaagaacgc tattcgtaaa ttaaaagagt ttcatagatg gcaacgtatc
    11521 gccaatagcc ttgatttaag ctataacgag cgttaccagt ttgatataaa atatcatccc
    11581 acgcgcagag aacaccttga aataagccga gaatgtgcct tagaggagct agacgctatc
    11641 aagtatgcta tcaaccaact gtctaagata gactacagaa agatattgat tgagtgttac
    11701 ttgattagtg agaaaaaaac tcagcaagac atcatagcag aacttaacaa aagtcaaagt
    11761 tggtactacg agactaagaa aagagctttg cttgaattcg cccagagtta taggaatggt
    11821 gtgttaataa caacagcata ggctcttgga tatatgcaac tatacaggta tagtttaaag
    11881 tgttataata actaaaagga ggtgctacta tgcacgcttt agaaaaaaat acgcaggtca
    11941 actttaaaac caacagtgat ttactagaga aagctaaagc tattatcgca gcccaaaacc
    12001 ttgacatgac agctagcttt aatttgtttt tagaaaacat tgtacaaaat aaagccttac
    12061 cttttgagac tgacaccgat aaagaacgag ctgaactact tgcaggttta cgagctgaaa
    12121 ttgctaaaag ttttgacgat ttagagcatg ggcgggtata caacgctagt gaagtgaggg
    12181 ctaaccttgg tatctgataa taaaacccat agccttatta tccctgaaac cgtacaagaa
    12241 caattacagg agataaaaag ctatattgaa actacttact tttcggagca ggcaggggct
    12301 aacaccgtca acaatattct ttacgggcta gaacgtcttg aatttttccc agaagcagga
    12361 tttaacgcag atgacagagt aggtgaaact atttacccgc cacataatac tcgttgtatt
    12421 gttttagggg actacctagc tttttatcac attttagaag acagaaaagc cgtttttgta
    12481 tctgatatta tccatagcaa acaagactat atcaaactgt ttaagaaaaa ataaaaacgc
    12541 aagatttaca gcaaactaaa aagcaccttt attgggtgct agttcttgcc tgctgaactc
    12601 attgattgac agagttgtaa attaaatttt tgcttacttt ttgctaactt tgaaggaaat
    12661 tttaatgtca actaatgttg acaaaaaaag tttaaaatgt tgatttaaag ctataaagac
    12721 ttgtaaagca acc
//
LOCUS       DATROD010000043        12517 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.043, whole genome shotgun sequence.
ACCESSION   DATROD010000043 DATROD010000000
VERSION     DATROD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12517
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.043"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..1471
                     /locus_tag="VBC74_001156"
     CDS             <1..1471
                     /locus_tag="VBC74_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEO6942108.1"
                     /translation="FYYDYVGALPKAEQRQANLYALALRANQFEKTGFKGLSRFIRMI
                     DKVLENENDLADVEVALPQNAVNLMTIHKSKGLEFKYVFILNIDKKFSMVDITSPLIL
                     SRNQGIGIKYVADMRHELEEEILPAVKVSMETLPYQLNKRELRLATLSEQMRLLYVAM
                     TRAEKKLYLVGKASQTKWADHYDLVSENNYLPLASRETFVTFQDWLLAVHETYKKQEL
                     FYDINFVSLEELTDHHIGMVNPSLPFNPDNKAENRQSEDIVHAISVLESVEQINQTYK
                     AAIELPTVRTPSQVKKFYEPILDIEGVDVMETITKTSVDFKLPDFSTSKKQDPAALGS
                     AVHELMQRIEMSSHVKMEDIQKALTEVNAETSIKAAIQIEKINYFFQENSLGKYIQEE
                     VEHLHREAPFAMLKEDPESGEKFVVRGIIDGYLLLENRIILFDYKTDKFVNPLELKER
                     YQGQMALYAEALKKSYEIERIDKYLILLGGKQLEVVKMD"
     gene            1484..2401
                     /locus_tag="VBC74_001157"
     CDS             1484..2401
                     /locus_tag="VBC74_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO6942109.1"
                     /translation="MIVEQKFGNGFTWINIEAEQLRTETSEIQAKYLDSEIITYALDD
                     YERAFMECSHIKGKEVLTIIFNTIDLKQKESYYETVPMTFCLSHDRLITVTRSRNSYM
                     LELLQKYLDRNPDVSPKKFLFAALTLITKQYFNVVSKIDREKDILNRQLREQTTNKRL
                     LAMSDLETGSVYLLTAANQNALVLEQLDVHPSQRFNSEVEKEQLSDALIEAHQLVSMT
                     QLNSQVLSQLSSTFNNVLNNNLNENLTGLNIISINLAIIAAITGFFGMNIPLPLTESR
                     SSWLIVIATSVLLWVIIAQILKRVFNLRK"
     gene            complement(2433..3809)
                     /gene="mnmE"
                     /locus_tag="VBC74_001158"
     CDS             complement(2433..3809)
                     /gene="mnmE"
                     /locus_tag="VBC74_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEO6942110.1"
                     /translation="MSITKEFDTIAAISTPLGEGAIGIVRISGTDALKIASKIYRGKD
                     LSAIQSHTLNYGHIVDPDKNEILDEVMLGVMLAPKTFTREDVIEINTHGGIAVTNEIL
                     QLILRHGARMAEPGEFTKRAFLNGRVDLTQAEAVMDLIRAKTDKAMDIAVKQLDGSLK
                     TLINNTRQEILNTLAQVEVNIDYPEYDDVEEMTTTLMREKTQEFQALMENLLRTARRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREEKAIVTDIEGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRDTDDIVEKIGVERSKKALEEADLVLLVLNSSEPLTLQDRSLLELSKESNRI
                     VLLNKTDLPQKIEVNELPENVIPISVLENENIDKIEERINDIFFDNAGMVEHDATYLS
                     NARHISLIEKAVDSLKAVNEGLELGMPVDLLQVDMTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
     gene            3937..5847
                     /locus_tag="VBC74_001159"
     CDS             3937..5847
                     /locus_tag="VBC74_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEO6942111.1"
                     /translation="MIILQGNKIERSFSGDVLFDNINIQVDQRDRIALVGRNGAGKST
                     LLKILVGEEAPTKGEINKKRDLSLSYLAQDSRFQSENTIFQEMLQVFDSLREVEKRLR
                     ELELQMGQVSGSDLEQLMKTYDILSEEFREKGGFTYESDIKAILNGFKFNSDMWEMPI
                     SELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIDTIAWLENYLVNYQGALIIVSHD
                     RYFLDKVATVTYDLTTHSLDRYVGNYSKFMDLKAEKIATEEKNFEKQQKEIAKLEDFV
                     QRNIVRASTTKRAQARRKQLEKMERLDKPNVEQKSANMTFHAGKVSGNVVLTLENAAI
                     GYEGVSLSEPIDLDVKKFDAIAIVGPNGIGKSTLIKSLVGQIPFIKGEAKLGANVETG
                     YYDQSQSNLTKTNTVLDELWNAFSTTPEVEIRNRLGAFLFSGDDVKKSVSMLSGGERA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIEFDGTLLFVSHDRYFINRVAT
                     KVLEISDKGSTLYLGDYDYYLTKKAELEELARLNEEEVSASKTEIDVTSDYETQKANQ
                     KEFRKITRRVAEIEARLEVLENDENNINGLMLETNDIGKLSDLQKELESIQEEQLLLM
                     EEWENLNMRLDL"
     gene            5997..6965
                     /locus_tag="VBC74_001160"
     CDS             5997..6965
                     /locus_tag="VBC74_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEO6942112.1"
                     /translation="MVTLSKEQHLDMFLKMQRIRDVDMKFNKLVRRGFVQGMTHFSVG
                     EEAASVGAIQDLTDSDIIFSNHRGHGQTIAKGIDIGGMFAELAGKATGTSKGRGGSMH
                     LANLEKGNYGTNGIVGGGYALAVGAALTQQYEGTDNIVIAFSGDSATNEGSFHESVNL
                     AAVWNLPVIFFIINNRYGISTDITYSTKIPHLYMRADAYGIPGHYVEDGNDLMAVYEK
                     MHEVINYVRSGNGPAIVEVESYRWFGHSTADAGVYRTKEEVDSWKAKDPVKRYRAYLI
                     ENEIATEEELAAIEAQVIKEVEEGVKFAEESPFPDMSVAFEDVFVD"
     gene            7040..8038
                     /locus_tag="VBC74_001161"
     CDS             7040..8038
                     /locus_tag="VBC74_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEO6942113.1"
                     /translation="MSETKVMALREAINVAMSEEMRKDEKVFLMGEDVGVYGGDFGTS
                     VGMLEEFGAKRVRDTPISEAAIAGSAIGAAQTGLRPIVDLTFMDFVTIAMDAIVNQGA
                     KTNYMFGGGLSTPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGLKVVAPGTVNESKAL
                     LKSSILDNNPVIFLEPKALYGKKEEVNMDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVMQAAEEVAEEGINVEVVDPRTLIPLDKELIIDSVKKTGKLILVNDAYKTGGFTGEI
                     ATMVAESEAFDYLDHPIVRLASEDVPVPYSRVLEQGILPDVAKIKDAIYKVVNKGK"
     gene            8165..9553
                     /locus_tag="VBC74_001162"
     CDS             8165..9553
                     /locus_tag="VBC74_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEO6942114.1"
                     /translation="MAVEIIMPKLGVDMQEGEILEWKKQVGDVVNEGDVLLEIMSDKT
                     NMEIEAEDSGVLLKITHGNGDVVPVTETIGCIGAEGEEVTEASSSENTSVEENATQVT
                     SEPEKVEETSEPSVPAATSGEKVRATPAARKLAREMSIDLALVSGTGANGRVHREDVE
                     SFKGAQPRITPLARRIAEDQGVDIAEITGSGIRGKIVKNDVLAAMSPQAAEAPVKTKA
                     TPTTEEKQLPEGVEVIKMSAMRKAISKGMTNSYLTAPSFTLNYDIDMTEMMALRKKLI
                     DPIMAKTGLKVSFTDLIGMAVVKTLMKPEHRYLNASLINDAQEIELHNFVNIGIAVGL
                     DDGLIVPVVHNADQMSLSDFVIASKDVIKKTQEGKLKSAEMSGSTFSITNLGMFGTKT
                     FNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRIVDGMNGAKFMVDL
                     KNLMENPFGLLI"
     gene            9613..11370
                     /gene="lpdA"
                     /locus_tag="VBC74_001163"
     CDS             9613..11370
                     /gene="lpdA"
                     /locus_tag="VBC74_001163"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEO6942115.1"
                     /translation="MAFDVIMPKLGVDMQEGEILEWKKNEGDTVNEGDVLLEIMSDKT
                     NMEIEAEDTGVLLKIVHQAGDVVPVTEVIAYIGEEGEEVGTSSPSADATITAEDGQSV
                     SGPAAPSQETVAAATPKEELAADEYDIVVVGGGPAGYYAAIRGAQLGGKIAIVEKTEF
                     GGTCLNVGCIPTKTYLKNAEILDGLKVAAGRGINLASTNYAIDMDKTVAFKNSVVKTL
                     TGGVRGLLKANKVEIFNGLGQVNPDKSVVIGDKVIKGRNVVLATGSKVSRINIPGIES
                     PLVLTSDDILDLREIPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAMDKE
                     VSLELQKILAKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVADKALLSIGRVPQMN
                     GLENLEPELEMERGRIKVNAYQETSIPGIYAPGDVNGTRMLAHAAYRMGEVAAENALG
                     GNKRKAHLDFTPAAVYTHPEVAMVGMTEEQAREQYGDILVGKNSFTGNGRAIASNEAH
                     GFVKVIAEPKYKEILGVHIIGPAAAELINEASTIMENELTVYDVAQSIHGHPTFSEVM
                     YEAFLDVLGEAIHNPPKRK"
     gene            11468..12457
                     /locus_tag="VBC74_001164"
     CDS             11468..12457
                     /locus_tag="VBC74_001164"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEO6942116.1"
                     /translation="MKYIVNTSNDPAYNVALEAYAFQKLTDIDEIFILWINEPAIIIG
                     RHQNTIQEINKEFIDKNGIHVVRRLSGGGAVYHDLNNLNYTIISNNTQEGAFDFQTFS
                     KPVIDTLAKLGVKAEFTGRNDLEINGQKFAGNAQAYYKGRMMHHGCLLFDVDMSVLGQ
                     ALKVSKDKIESKGIKSVRARVTNIVDHLSDKITVQEFSDAILAQVKEEYPEMDEYVLS
                     DAELSEIQAMRDNQFATWDWTYGKAPEYTIERGVRYPAGKITTYANVENSTIKSVKIF
                     GDFFGVKPVDDIEKMLEGVRYDYKDVLAALKTVDTSQYFSRMTPEEITKAIVD"
BASE COUNT         4118 a         2027 c         2549 g         3823 t
ORIGIN      
        1 tttttattat gattatgtag gagctctgcc taaagctgag caacgtcaag ccaatctata
       61 tgccttggca cttcgagcaa atcagtttga aaaaacaggt ttcaagggtt tatcacgttt
      121 tattcgaatg attgataaag ttttagaaaa tgaaaatgat ttggcagatg ttgaggtggc
      181 attaccgcaa aatgctgtta acctaatgac tatacacaaa tcaaagggtc ttgagtttaa
      241 gtatgtattt attttaaata ttgataagaa attttcaatg gtagacataa cttcccctct
      301 tattttaagc cgaaatcaag gaattggtat taaatatgtt gctgatatgc gacatgaact
      361 cgaagaagag atactgcctg cagtaaaggt tagcatggaa acacttcctt accaacttaa
      421 taaaagagag ttacgcttag ctactttatc agagcaaatg cgtttacttt acgtagctat
      481 gacacgtgca gagaaaaaat tatatctggt tggtaaggct agtcaaacca aatgggctga
      541 tcattatgac ttagtatctg aaaataatta tctcccatta gctagtcgtg agacttttgt
      601 gacctttcaa gattggttac tggctgttca tgaaacctat aagaaacaag agttgtttta
      661 tgacatcaac ttcgtatcat tagaggaatt aacagatcac cacataggga tggttaatcc
      721 tagtctacct ttcaatcccg ataataaggc agagaatcgt cagtctgaag atatcgtgca
      781 cgcgattagc gtgttagaat ctgttgaaca gattaatcaa acttataaag cggctattga
      841 gttaccgaca gtaagaacac caagtcaagt caaaaaattc tatgagccta ttctagatat
      901 agaaggcgtt gatgtgatgg aaactattac taaaacatca gtagatttta agttacctga
      961 tttttctaca agtaaaaagc aagacccagc agctttagga tctgctgtac atgaattaat
     1021 gcaacgcata gagatgtcat ctcatgtcaa aatggaagat attcaaaaag ccttgacaga
     1081 agtgaatgct gaaacaagta tcaaagcggc tattcagatt gaaaaaatca actatttttt
     1141 ccaagaaaac tcactcggta aatatataca agaagaagta gaacatctac atcgagaggc
     1201 accttttgcc atgttaaaag aagatccgga gagtggtgag aaattcgttg tacgtggtat
     1261 tattgatggg tatctactat tagaaaatcg gataattcta tttgattata agactgataa
     1321 atttgtcaat cctctggaat taaaagaacg ctatcagggg caaatggctt tatatgcaga
     1381 agctttaaaa aaatcttacg agatagaaag gatagacaaa tacttaattc ttttaggtgg
     1441 taaacagcta gaagtggtaa aaatggatta aagaaaggaa aaaatgatag tcgaacaaaa
     1501 atttggaaat ggttttactt ggattaacat tgaagcagag caattgcgaa cagaaacatc
     1561 ggaaattcaa gcaaaatatt tggatagtga gattattaca tatgctctgg atgattatga
     1621 acgagccttt atggaatgtt ctcatattaa aggaaaagaa gtcttgacga ttatttttaa
     1681 tactattgat ctaaagcaga aagaaagtta ttatgagacc gttccgatga ccttttgctt
     1741 atcacacgac cgtttgataa cggtgacacg gtctcgaaat agttatatgc tagaactatt
     1801 gcaaaaatat ctagaccgta atccagatgt ctctcctaaa aaatttttat ttgcagcttt
     1861 aactttgatt acaaaacaat attttaatgt tgtatcgaaa attgatagag aaaaggatat
     1921 tctcaaccgt caattgagag aacaaacaac caataagcgc ttattagcca tgagtgacct
     1981 agagacaggc tccgtttatc ttttgacagc tgccaatcaa aatgcattgg tattagagca
     2041 attagatgtt catcctagtc agcgatttaa ttctgaagtg gaaaaagaac agttatcaga
     2101 tgctctcatt gaagcgcatc agttagtcag tatgacacaa ttaaacagtc aggtactttc
     2161 acaattatca tctactttta ataatgtctt aaataataac ttgaacgaaa acttaacagg
     2221 tttgaatatc atttcgatta atttagcgat tattgcagca attactggct tttttgggat
     2281 gaatattcca ttacctctta cagaatcacg gagttcatgg ttaattgtta ttgctacttc
     2341 ggtcttactt tgggttatta ttgctcaaat tttaaaacga gtttttaact taagaaaata
     2401 aaaatgttca gtgcgtaagc accgaacatt ttttatttac ctaagcagaa ttggctgaag
     2461 agctgagtta ttagctcatc tggagctgca tccccagtga tttctccaag tatttcccaa
     2521 gttctagtca tatcgacttg taagagatca acaggcattc ctaattccaa gccctcattg
     2581 acagctttta aactatcaac ggctttttca attagtgaga tatggcgagc attcgataga
     2641 taagtagcgt catgttctac cataccagca ttgtcaaaga aaatatcgtt aatacgttct
     2701 tcgattttat cgatgttttc attttctaaa accgaaattg gaataacatt ttcaggtaat
     2761 tcgttaactt ctattttttg aggaagatcc gttttattga gtaatacgat acgattactt
     2821 tctttactta gttctagaag ggagcggtct tgtaatgtta gtggttcgga actattgaga
     2881 accaacaaca caaggtcggc ttcttctaaa gccttctttg aacgttcaac accaattttt
     2941 tcaacgatat catctgtatc acggatacca gcagtgtcaa ctagttttaa aggaactcct
     3001 ttaatattga cgtattcttc aataacatca cgagttgttc cttcaatatc tgtcacaatc
     3061 gccttttctt cacgtaagag gttatttagt aagctggatt taccgacatt tggacgacca
     3121 atgattgctg ttgaaagtcc ttctcgaaga atttttccac gtcgggcagt tcgaagtaaa
     3181 ttttccataa gtgcttgaaa ctcttgtgtt ttttcacgca tcagtgttgt agtcatctct
     3241 tcaacgtcat catattcagg gtaatcgata ttaacttcaa cttgggctag agtatttaga
     3301 atttcttgtc gagtgttatt gattagtgtc tttaaagaac catctaattg cttaacggca
     3361 atatccattg ctttatctgt tttagctcta ataagatcca taacagcttc agcctgagtc
     3421 aagtcaactc gaccgtttaa aaaagcacgt ttagtaaatt ctcctggttc agccattcgt
     3481 gctccatgtc gtaaaattaa ctgaagaatt tcgttagtaa ctgcaattcc tccatgcgta
     3541 ttaatttcaa taacatcttc tcttgtgaat gtttttggag caagcataac gcctaacata
     3601 acttcatcca agatttcatt tttatctggg tccacaatat gaccatagtt taaggtatgg
     3661 gattggatag cggatagatc ttttccacga taaatttttg atgctatctt taaggcatct
     3721 gtaccagata tacggacaat accaatagct ccctcaccga gaggagttga aatagctgcg
     3781 atggtatcaa attcttttgt aatagacata aagtctctct ctttcttggg aattactaac
     3841 attcattgta acctaaagcc ctaaaaggtg caatcatttg attaaaagag aagatttcca
     3901 taatggtttt atttgaaggt atgctataat aaaactatga ttatactaca aggaaacaaa
     3961 attgagcgct cattttcagg tgatgtgctt tttgataata ttaatattca agttgatcaa
     4021 cgtgatcgta ttgctcttgt tggacgtaat ggtgctggca aatcaacgct tcttaaaatt
     4081 ttagttggtg aagaggctcc gacaaaagga gaaataaata aaaagcgtga tttgagtttg
     4141 tcctacttgg cacaagatag tcgttttcaa tctgaaaata caattttcca agaaatgtta
     4201 caagtttttg attcattaag agaagtagag aagcgcttac gtgaattgga attacaaatg
     4261 ggacaagttt caggaagtga cctggaacaa ttgatgaaaa catacgatat tttgtcagaa
     4321 gagtttcgtg aaaaaggtgg ctttacctat gaatcagata taaaggctat tttaaatggc
     4381 tttaagttta acagtgatat gtgggaaatg cctattagtg aattgtctgg tgggcaaaat
     4441 acacgattag cgcttgcaaa aatgctactt gaaaagccag aattacttgt tttagatgaa
     4501 ccaacgaacc atttagacat tgatacgatt gcttggttag aaaattatct tgtaaattat
     4561 caaggagccc ttattattgt cagccatgac cgctattttt tagacaaggt agctacagta
     4621 acttatgatt taacgacaca ttctctagac cgctatgtag gcaattattc gaaatttatg
     4681 gatcttaagg cagagaaaat agcgactgaa gaaaagaatt ttgaaaagca acaaaaagaa
     4741 attgctaagt tagaagattt tgtacaacgt aacattgtta gggcttcaac cactaaacga
     4801 gcacaagctc gtcgtaaaca attagaaaag atggaacgac tcgacaaacc gaatgtagag
     4861 caaaaatcag ctaatatgac ttttcatgct gggaaagtct cagggaatgt tgttttaaca
     4921 cttgagaatg cagctattgg ttatgaaggt gtttctttat cggagccaat cgatttggac
     4981 gtgaagaaat ttgatgctat tgcaattgta ggtccaaatg gtatcgggaa atcaacttta
     5041 atcaaatcgc ttgttgggca aattcctttt atcaaaggag aagcaaaatt aggagcaaat
     5101 gtcgaaactg ggtattacga tcaaagtcaa agtaatttaa caaaaacgaa tactgtttta
     5161 gatgagttat ggaatgcttt ttcaacaact ccagaagttg aaattagaaa ccgtttagga
     5221 gcttttcttt tttcaggtga tgatgttaaa aaatcagttt caatgctatc cggtggtgaa
     5281 cgagcgcgtt tgctattggc aaaattatct atggaaaata ataatttcct catattagat
     5341 gagccaacaa accacttgga tattgatagt aaagaagtct tagaaaatgc actcatcgaa
     5401 tttgatggca cattactttt tgttagtcat gatcgctatt ttattaatcg tgtggcaact
     5461 aaggttctcg aaatttctga taagggaagt actctctatc taggagatta tgattattac
     5521 ctcactaaga aagccgaact agaggaatta gcccgtttaa atgaagaaga agtgagtgct
     5581 tcaaaaacag aaatcgacgt tacatcagat tatgagacac aaaaagcaaa tcaaaaggaa
     5641 tttaggaaaa ttacccgtcg cgttgcagaa attgaggcac gtttggaagt tcttgaaaat
     5701 gatgaaaaca atatcaatgg tttaatgtta gaaaccaatg acatcggaaa actctctgat
     5761 ttacaaaaag agctggaatc aatccaagaa gaacaattgc tattaatgga agaatgggaa
     5821 aatctaaata tgagactaga tttgtgagaa aatttcaaaa acaatagttt gtgaaaaaaa
     5881 gtacatttta aattgagatg ccttttacga aaacaaagaa agcggttcaa attcttgcat
     5941 cttgtgaaaa aaacttatac aatgattaag taataaagat ttttttggag gttagaatgg
     6001 taactctatc taaagaacaa cacttggata tgtttctaaa aatgcaacgt attcgtgatg
     6061 ttgatatgaa atttaacaaa ttggttcgtc gtggtttcgt ccaagggatg actcacttct
     6121 ctgttggtga agaagctgct tcagtaggtg ctatccaaga tttaacagat tcagatatca
     6181 ttttctcaaa ccaccgtgga cacggacaaa ctattgcaaa aggtattgat attggtggaa
     6241 tgtttgctga acttgctggt aaagcaacag gaacatcaaa aggtcgtgga ggctcaatgc
     6301 acttagcaaa ccttgaaaaa ggtaactatg gaacaaacgg tatcgtcggt ggaggatatg
     6361 cgcttgcggt aggtgctgcc cttacacagc aatatgaagg aactgataac attgttatcg
     6421 ccttttcggg tgactcagcg actaacgaag gctcattcca tgagtcagtt aacttagctg
     6481 ctgtttggaa cttaccagtt atcttcttta tcatcaacaa ccgttatggt atctcaacag
     6541 acatcacata ctcaactaaa attcctcacc tttacatgcg tgctgatgcg tacggtattc
     6601 cagggcatta tgttgaagat ggtaatgatc ttatggctgt ttacgagaaa atgcatgaag
     6661 ttatcaatta cgtgcgttca ggaaatggac cagctattgt agaggttgaa tcataccgtt
     6721 ggtttggtca ttcaactgct gatgctgggg tataccgtac taaagaagaa gttgattcat
     6781 ggaaagctaa agatccagta aaacgctacc gtgcatactt aattgaaaat gaaatcgcta
     6841 ctgaagaaga gcttgcagct attgaagcac aagtgattaa agaagttgaa gaaggggtta
     6901 aattcgctga agaaagtcca ttccctgata tgtctgttgc atttgaagac gtttttgtgg
     6961 actaaaataa cctaaagtag atgagattta tctcgttcta ttattcaatt gattaaaaaa
     7021 cggatattcg gaggaaataa tgagcgaaac taaagttatg gctttgcgcg aagcgattaa
     7081 tgtcgctatg agcgaagaaa tgcgcaaaga tgaaaaagta tttttaatgg gtgaagatgt
     7141 aggtgtatat ggaggagact tcggtacatc tgttggtatg ttagaagagt ttggtgctaa
     7201 gcgcgttcgc gacacaccaa tctcagaggc tgcgattgca ggttcagcta tcggtgctgc
     7261 acaaacaggt ttgcgtccta tcgttgattt gacattcatg gatttcgtaa caatcgcaat
     7321 ggatgctatc gttaaccaag gtgctaaaac gaattatatg tttggtggag gtctatcaac
     7381 tccagttact ttccgtgtgg catcagggtc aggtattggt tcggctgcac aacactcaca
     7441 atcattagaa gcttggttaa cacacattcc aggtctaaaa gttgttgcac ctggtactgt
     7501 taatgagtca aaagcacttt tgaaatcttc tattcttgac aataacccag ttatcttctt
     7561 agagccaaaa gctctctatg gtaagaaaga agaagttaat atggatcctg atttctatat
     7621 tccacttggt aaaggggata ttaagcgtga aggtactgac ttaacaattg tttcttatgg
     7681 ccgtatgctt gagcgtgtta tgcaagctgc tgaagaagtt gctgaggaag gtattaacgt
     7741 tgaggttgtt gatccacgta cacttattcc gcttgataaa gaattaatta tcgattctgt
     7801 taagaaaact ggtaaattga tcttggttaa cgatgcttat aaaactggtg gtttcactgg
     7861 tgaaattgca actatggttg ctgaaagtga agcatttgat taccttgatc atcctatcgt
     7921 gcgtcttgca agtgaagatg ttccagtacc gtattcacgt gtacttgaac aaggaatctt
     7981 acctgatgta gcaaaaatta aagatgctat ctacaaagta gttaacaaag gtaaataatt
     8041 cataaaacaa agagggactg aggaatgagt gaaaacttag cctctttcct tgtttatagg
     8101 ttttaattta tatcgttaga tgaaacatat atttgacgat atagtttagt gtgaaaggaa
     8161 aactatggct gtcgaaatta ttatgcctaa actcggggtt gacatgcaag aaggcgaaat
     8221 ccttgagtgg aaaaaacagg taggtgatgt tgttaatgaa ggtgacgtcc tacttgaaat
     8281 tatgtcagat aaaacaaata tggagattga agcagaagac tcaggtgttt tactaaaaat
     8341 cacacatggg aatggagatg ttgttcctgt aactgagaca attggctgta tcggtgctga
     8401 aggtgaagaa gtaacagaag catcttcttc tgaaaataca tcagttgaag aaaacgcgac
     8461 acaagtaact tcagaaccag aaaaagttga agaaacatca gaaccaagtg tgccagcagc
     8521 tacaagtggt gaaaaagtac gtgccactcc tgcagctcgt aaattagcaa gagagatgtc
     8581 tattgacctt gcactagtat ctggaacagg ggcaaatggt cgcgttcaca gagaagatgt
     8641 tgaaagcttc aagggtgctc aaccacgtat tacaccattg gcacgtcgaa ttgctgaaga
     8701 tcaaggagtt gatatagcag aaatcactgg cagtggtatt cgtggtaaaa ttgttaaaaa
     8761 tgatgttttg gctgctatgt caccgcaagc agcagaggct cctgtaaaaa cgaaagctac
     8821 accaacaaca gaagaaaaac aattaccaga aggtgttgaa gtcattaaga tgtctgcaat
     8881 gcgtaaggct atttctaaag gtatgactaa ctcttacctc actgctccaa gctttacact
     8941 taactatgat attgatatga ctgaaatgat ggctcttcgc aagaaattaa ttgatccaat
     9001 catggctaaa acaggcttga aagtcagctt tactgatttg attggaatgg ccgttgttaa
     9061 gacattgatg aaaccagagc atcgatacct aaatgcttca ttaattaacg atgcacaaga
     9121 aattgagctt cataattttg ttaatattgg aatcgcagtt ggtcttgatg atggtttgat
     9181 tgttcctgtt gttcacaatg ctgatcaaat gtcactttct gactttgtta tagcatctaa
     9241 agatgttatt aagaaaactc aagaaggtaa attaaaatca gctgaaatgt caggttcaac
     9301 attctcaatt actaacttag gtatgtttgg tactaagacg tttaacccaa ttatcaatca
     9361 accgaactca gcaatccttg gagtaggtgc tactattccg actccaacag tggttgatgg
     9421 tgaaattgta gctcgaccaa tcatggcaat gtgtttaaca attgaccacc gtattgttga
     9481 tggaatgaat ggtgctaagt ttatggttga tcttaagaac ttaatggaaa acccatttgg
     9541 tttacttatt tgattccgta ggtcttaatt tgatataata agttattcga aaaacgaaag
     9601 aggaaataaa aaatggcttt tgatgtaatt atgccaaaac ttggggttga catgcaagaa
     9661 ggcgaaatcc ttgagtggaa aaaaaatgaa ggtgacaccg ttaatgaagg tgatgtcctt
     9721 cttgaaatca tgtctgataa aaccaatatg gaaattgaag cagaagacac tggtgtactt
     9781 ttaaaaattg tacatcaggc aggagatgtt gttcctgtca ctgaagtgat tgcctatatc
     9841 ggagaagaag gtgaagaagt aggtacgtca tcaccttcgg ctgatgcaac tatcacagct
     9901 gaagatggtc aatcagtttc tggacctgct gctccttcac aagagacagt tgcagcagca
     9961 actcctaaag aagaacttgc agctgatgag tacgatatcg tagtcgtagg tggtggccct
    10021 gcaggttatt acgcagcaat ccgcggtgct caacttggag gaaaaattgc cattgttgag
    10081 aaaactgaat ttggcggtac gtgtttgaat gttggttgta tccctacaaa aacatatctt
    10141 aaaaatgctg aaatcctaga tggtttgaaa gtagcagctg gacgtggtat caaccttgct
    10201 tcaactaact atgcaattga tatggataag actgttgcct ttaaaaattc tgtagttaag
    10261 acattgactg gtggtgtacg tggtctcttg aaagcgaaca aagttgaaat tttcaatgga
    10321 cttggacaag taaaccctga taaatctgtt gttattggcg ataaagttat caaaggtcgt
    10381 aacgtcgtcc ttgcgactgg ttctaaggta tctcgtatta atattccagg tattgaatca
    10441 ccattagtat taacatcaga tgatattctt gatttacgtg aaattcctaa gtcacttgct
    10501 gttatgggtg gtggtgtcgt aggtattgag cttggtcttg tatgggcttc atatggtgtt
    10561 gacgtaactg ttattgaaat ggctgatcgc atcattccag caatggataa agaagtatcg
    10621 ctagaacttc aaaaaatcct tgctaagaag ggaatgaaga ttaaaacatc agttggtgtt
    10681 tctgaaattg ttgaagccaa taatcaatta acgcttaaat taaacaacgg cgaagaagtt
    10741 gttgctgata aagctcttct ttcaattgga cgtgttccac aaatgaatgg tcttgaaaat
    10801 cttgagcctg aacttgagat ggaacgtgga cgtattaaag ttaatgctta tcaagaaact
    10861 tcaattccag gtatctatgc accaggtgat gttaatggaa ctcgtatgtt agctcatgct
    10921 gcataccgta tgggtgaagt agctgctgaa aacgcactcg gcggtaataa gcgtaaagca
    10981 catctagatt tcactccagc agctgtttat actcatcctg aagtagcaat ggtggggatg
    11041 acagaagagc aagcacgtga acaatatggt gacatattag ttggtaaaaa tagctttaca
    11101 ggcaatggtc gtgctatcgc ttcaaacgaa gcacatggtt ttgtaaaagt aattgctgaa
    11161 cctaagtata aagagattct tggtgttcac attattggtc cagctgcagc ggaattgatt
    11221 aatgaagctt ctacgattat ggaaaatgag ttgactgtct atgatgtagc acaatctatc
    11281 catggtcacc caacattctc tgaagttatg tacgaagctt tcttagatgt tctcggtgaa
    11341 gctattcaca accctccaaa acgcaaataa ttttgtttaa tatatttgag attgagcttt
    11401 tatagcttca atctcttttc acgtatgtat tgtctgaata ttataaaata ttagttagga
    11461 atttaaaatg aaatatatcg ttaacacgtc taatgatccg gcttacaatg ttgctttaga
    11521 agcttacgcc tttcaaaagt tgacagatat cgatgaaatc tttattcttt ggattaatga
    11581 accggctatc attattggac gccatcaaaa tacaattcaa gaaattaaca aagagttcat
    11641 tgataaaaat ggcatccatg ttgtccgtcg tttgtcagga ggcggagcag tttatcatga
    11701 cttgaataat ttaaactaca ctattatttc taataataca caagaaggtg cttttgattt
    11761 ccaaactttt tcaaaaccag ttattgatac tcttgctaaa ttaggtgtta aagcagaatt
    11821 tactggtcgt aatgatttag aaatcaatgg acaaaaattt gcaggaaatg ctcaagctta
    11881 ttacaaaggt cgtatgatgc atcatggttg tcttttattt gatgttgata tgtcagtctt
    11941 agggcaagca ctcaaagtat ctaaagacaa aattgaatct aaagggataa aatctgtacg
    12001 cgcacgtgtt acaaatattg ttgaccatct ttcagataaa attacagtac aagaatttag
    12061 cgatgccatt ttagctcaag tgaaggaaga atatccagag atggatgaat atgttttgag
    12121 tgatgccgag ttatcagaaa tccaagctat gcgcgataat caatttgcta catgggattg
    12181 gacgtatggt aaagcacctg aatacaccat tgaacgtggt gtccgctatc cagctggaaa
    12241 aattacgact tatgctaatg ttgaaaattc aacgataaaa tctgtcaaaa tctttggtga
    12301 cttctttgga gtgaaacctg tagatgatat tgaaaaaatg ttggaaggtg tccgctacga
    12361 ttacaaagat gttttagcag cgcttaaaac agttgataca agtcaatact tctcacgtat
    12421 gacacccgaa gagattacaa aagctattgt agactaaaat caaaaaacct agtcaaagtc
    12481 tttgcctttg gctaggtttt ttgtatggtt aagtggc
//
LOCUS       DATROD010000044        12368 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.044, whole genome shotgun sequence.
ACCESSION   DATROD010000044 DATROD010000000
VERSION     DATROD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12368
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.044"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..147
                     /locus_tag="VBC74_001165"
     CDS             <1..147
                     /locus_tag="VBC74_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO6942117.1"
                     /translation="KGYISSDTVMVVRKNGKIFDYVLPGEPVRLWEVATEEKVEEVLM
                     ELDK"
     gene            360..1052
                     /locus_tag="VBC74_001166"
     CDS             360..1052
                     /locus_tag="VBC74_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO6942118.1"
                     /translation="MSKVRLYIARHGKTMFNTIGRAQGWSDTPLTTFGELGIKELGWG
                     LKASNISFKEAFSSDSGRTLQTMEIILREVQQENIPYTRDKRIREWCFGSLDGGYDGD
                     LFNGVLPRVSNGDMSHLTHEEIANLICQVDTAGWAEPWAILSNRILSGFTAIAKKIED
                     IGGGNAIVVSHGMTIATFLWLIDHSTPRSLGLDNGSVSVVDFEDGTFSIQSIGDMSYR
                     EKGREILEKTLQ"
     gene            1049..1801
                     /locus_tag="VBC74_001167"
     CDS             1049..1801
                     /locus_tag="VBC74_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEO6942119.1"
                     /translation="MKKNKIIRFSLVGVLLAILCFSLFALLKPNSQQSSSQKLRNEDI
                     KKTSSQKRNKKLRLPAVSSKDWNLILVNRDHKHEELSPDVVPVENIYLDKRITKQATQ
                     FLEAARAIDSREHLISGYRSVAYQEKLFNSYVTQEMTSNPNLTRGQAEKLVKTYSQPA
                     GASEHQTGLAMDMSTVDSLNESDPRVVSQLKKIAPQYGFVLRFPDGKTAETGVGYEDW
                     HYRYVGVESAKYMVKHHLTLEEYITLLKENNQ"
     gene            1798..2373
                     /locus_tag="VBC74_001168"
     CDS             1798..2373
                     /locus_tag="VBC74_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEO6942120.1"
                     /translation="MRKRFSLLNFIVVTFIFFFFILFPLLNHKGKVDANSRQSVTYTK
                     EEFIQKIVPDAQDLGKSYGIRPSFIIAQAALDSDFGEKILANKYHNLFGLLAEPGTPS
                     ITLNDSSTGKKQEKQFTHYKSWKYSMYDYLAHIKSGATGKKDSYTIMVSVKNPKTLVQ
                     KLQDSGFDNDKKYAKKMTEIIDLYDLTRYDK"
     gene            2468..3550
                     /gene="hrcA"
                     /locus_tag="VBC74_001169"
     CDS             2468..3550
                     /gene="hrcA"
                     /locus_tag="VBC74_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEO6942121.1"
                     /translation="MCKSANFRKTERGDVFVITQRQNDILNLIVELFTQTHEPVGSKA
                     LQRTIDSSSATIRNDMAKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQD
                     IYHVIKAFDFEAFKLEDMLQKASHILSEMTGYTSVILDVEPARQRLTGFDVVQLSNHD
                     ALAVMTLDESKPVTVQFAIPRNFLTRDLIAFKAIVEERLLDGSVMDIHYKLRTEIPQI
                     VQKYFVTTDNVLQLFDYVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLR
                     QGLKEGEMASVQVADSQEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLV
                     NIIGKVLAAKLGDYYRYLNSNHYEVH"
     gene            3553..4125
                     /gene="grpE"
                     /locus_tag="VBC74_001170"
     CDS             3553..4125
                     /gene="grpE"
                     /locus_tag="VBC74_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEO6942122.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEEIVEEVIEEIP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            4306..6135
                     /gene="dnaK"
                     /locus_tag="VBC74_001171"
     CDS             4306..6135
                     /gene="dnaK"
                     /locus_tag="VBC74_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEO6942123.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVEEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIQSNSGLTDEEIDKMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKKAQESGNLDDMKAKLEALNEKAQ
                     ALAVKLYEQAAAAQQAAQGAEGAQSADSSSKGDDVVDGEFTEK"
     gene            6424..7539
                     /gene="dnaJ"
                     /locus_tag="VBC74_001172"
     CDS             6424..7539
                     /gene="dnaJ"
                     /locus_tag="VBC74_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEO6942124.1"
                     /translation="MNNTEFYDRLGVSKDASQDEIKKAYRRMSKKYHPDINKEAGAEE
                     KYKEVQEAYETLSDTQKRAAYDQYGAAGANGGFGGFDGGGFGGFEDIFSSFFGGGGMR
                     NPNAPRQGDDLQYRVNLSFEEAIFGAEKEVSYNRESSCHTCSGSGAKPGTSPVTCQKC
                     HGSGVINVDTQTPLGTMRRQVTCDVCQGSGQEIKEKCPTCHGTGHEKKTHKVSVKIPA
                     GVETGQQIRLTGQGEAGFNGGPYGDLFVIINVLPSQQFERNGSTIYYTLNIGFVQAAL
                     GDTIDIPTVHGAVEMSIPAGTQTGKTFRLRGKGAPKLRGGGQGDQHVTVNIVTPTKLN
                     DAQKEALHAFAEASGDKMVHPKKKGFFDKVKDALDVD"
     gene            7657..8904
                     /locus_tag="VBC74_001173"
     CDS             7657..8904
                     /locus_tag="VBC74_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEO6942125.1"
                     /translation="MVTKVEEIRSYLIASIQNGKLAPGDRLPSIRQLANQFSCNKDTV
                     QRVLMELRFDNYIYAKPRSGYYVFDSHQEEVEEGVSLPNSEIANIAYDDFRLCLNETL
                     IGREDYLFNYYYRQEGLLDLSKAVAKLMEETGVYVPLDDIVITAGTQQALFILTQVTF
                     PNRKSRVLIEEPTYPRMIELIKTQNLPYETIPRGTHGIDFQRLEEIFQTQSIKFFYVI
                     PRMHNPLGTSYNPVEMKRLIEMAEKYDVYIVEDDYMSDFASQSPLHYYDTHGRVIYLK
                     SFSKAIFPALRLAAICLPQALKSTFMAYKKLMDYDTNLILQKALALYIENGLYAKNSQ
                     YLKYRYQKDLANSKSILADHPNLPPYSLHHDSVLFDCSKLDNFKILRQYGDTLENYFC
                     QKSHQSLLQVKNDSCLKQFLGSL"
     gene            8975..9751
                     /gene="truA"
                     /locus_tag="VBC74_001174"
     CDS             8975..9751
                     /gene="truA"
                     /locus_tag="VBC74_001174"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEO6942126.1"
                     /translation="MTRYKAQISYDGSAFSGFQRQPNCRTVQEEIERTLKRLNSGNDV
                     IIYGAGRTDAGVHAYGQVIHFDLPQARDVEKLRFGLDTQCPDDIDIVKVEQVSDDFHC
                     RYDKHIKTYEFLVDIGRPKNPMMRNYATHYPYPVIIELMQEAIKDLVGTHDFTGFTAS
                     GTSVENKVRTIFDAKIQFEASKNLLIFTFTGNGFLYKQVRNMVGTLLKIGNGRMPISQ
                     IKTILQAKNRDLAGPTAAGNGLYLKEIIYEDEECFSNFRK"
     gene            9714..10472
                     /locus_tag="VBC74_001175"
     CDS             9714..10472
                     /locus_tag="VBC74_001175"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEO6942127.1"
                     /translation="MKTRNVLAISGNDIFSGGGLHADLATYVVNKLHGFVAVTCLTAM
                     SDKGFEVIPIEASILKQQLESLKDVEFGSIKLGLLPNVETAQVVLEFVKSKQECPVVL
                     DPVLVCKENHDLEVSQLREQLIAFFPYADVITPNLVETQLLTGLSIENLDQMKIAAEK
                     LYDMGAKHVVIKGGNRLNAEEATDLYYDGERFETYVFPVVDANNTGAGCTFASSIASQ
                     LAMGKNVEDAVKMSKGFVYQAIKASDKYGVVQHF"
     gene            10482..10946
                     /locus_tag="VBC74_001176"
     CDS             10482..10946
                     /locus_tag="VBC74_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6942128.1"
                     /translation="MRKEKTSQLVQLAIVTAISIVLGMFISIPTPTGFLTLLDAGIFF
                     AAFYFGKKEGAVVGALAGFLIDLLKGYPNWMFFSLLIHGAQGYLAGLPGRRRLLGLIS
                     ATLVMVLGYAIASGLMYGWGAVLPDIPGNIMQNMVGMVVGFALNKSLERVKK"
     gene            10943..11512
                     /locus_tag="VBC74_001177"
     CDS             10943..11512
                     /locus_tag="VBC74_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEO6942129.1"
                     /translation="MILDYNKLKQETKAIVVDIIERSALKKGQIFVLGLSSSEVSGGL
                     IGKNSSSEIGEIIVEVILKELHSRGIYLAVQGCEHVNRALVVEAELAERQQLEVVNVV
                     PNLHAGGSGQVAAFKLMTSPVEVEEIVAHAGIDIGDTSIGMHIKRVQVPLIPISRELG
                     GAHVTALASRPKLIGGARAGYTSDPIRKF"
     gene            complement(11549..>12368)
                     /locus_tag="VBC74_001178"
     CDS             complement(11549..>12368)
                     /locus_tag="VBC74_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEO6942130.1"
                     /translation="DHLNVEEVLFTFFTKLISILLLIIAFVIVRQVINYLFEKTVNRS
                     LAFSRQKVARQKTLAKLSHNVLNYTLYFFLFYWILSILGVPISSLLAGAGIAGVAIGL
                     GAQGFLSDVVNGFFILLENQFDVGDIINVGAVSGTVTNVGIRTTQIHDFDGTLHFIPN
                     RNITIVSNKSRSNMRAQIDIPLFVHTNLDQISDIVTKINEEYVSKHPAIVGEPTVFGP
                     TTNANGQFVYRINIFTQNGAQFDIYAEFYKLYQKAILEEGIDLPTYNFSNNHSR"
BASE COUNT         4048 a         1918 c         2632 g         3770 t
ORIGIN      
        1 aaaggatata tcagcagtga tacagtgatg gttgtgcgta agaacggaaa gatatttgat
       61 tatgtgttac ctggtgagcc tgtaagattg tgggaagttg cgacagagga aaaagtggaa
      121 gaagtgttga tggaattaga taaataaaaa aagacttttc caagtccaaa aatcagatgc
      181 tccccagcgc aaaaattact acgttattta ctacgttgtt tttaaaatca gtgtaattga
      241 ccagataatg taaggttgta aaatgttgat ttatcaagtg attgggcaat gatagcaaat
      301 gataccatat taaggttctt ttttgttata atataataaa atgcttgtga ggtgtcagta
      361 tgagtaaagt tcgtttatat atcgctcgtc atggtaaaac aatgtttaat acaattggtc
      421 gtgctcaggg gtggagtgat actcctttaa ctacttttgg agaactcggg attaaagagc
      481 ttggttgggg actaaaggct tcaaatatta gttttaaaga ggctttttct agtgatagtg
      541 gacgtacttt acaaactatg gaaattattc tcagggaagt gcaacaagaa aatattccct
      601 atacgagaga taaacgaatc cgcgagtggt gctttggtag tttggatgga ggatatgatg
      661 gcgacctttt taatggcgtt ctgcctcgag tttcaaacgg agatatgagc catttgacac
      721 acgaagaaat tgctaatttg atttgtcaag ttgatacggc tggctgggca gaaccgtggg
      781 caatattgag taaccgtatt ctgtcaggct tcactgctat agctaaaaaa atagaggata
      841 ttgggggagg taatgcgatt gttgtcagtc atgggatgac tattgcgact tttttatggt
      901 taatcgacca tagtacccct cgtagtttag gcttagataa tggtagtgtc tcagtcgttg
      961 attttgaaga tggaacattt tctattcaat ctattgggga tatgtcctat cgagaaaaag
     1021 gaagagaaat tttagaaaaa acattgcaat gaaaaaaaat aaaattatcc gattcagttt
     1081 agttggtgtt ctacttgcga tactatgctt tagtcttttt gctttattga agcctaacag
     1141 tcaacaatca tcatctcaaa agttgaggaa tgaggatata aaaaagacat cctctcaaaa
     1201 aagaaataag aaattacgat taccagctgt atcatcaaaa gattggaact tgattttggt
     1261 caatcgtgac cataaacatg aagaattaag tccagatgtg gtgcctgttg aaaatattta
     1321 tttggataaa cgtattacga agcaagctac tcagttttta gaggctgcta gagcaattga
     1381 ttcacgagaa catttaattt cgggttatcg tagtgttgcc tatcaggaga agttgttcaa
     1441 ttcttatgtt actcaagaga tgactagtaa ccctaatttg acgaggggac aagcagaaaa
     1501 gttggtaaaa acttactctc agcctgcagg tgctagtgaa caccagactg gattagcgat
     1561 ggatatgagt actgtagatt ctttgaatga gagcgatcct agagtagtca gtcagttgaa
     1621 aaagatagct ccacaatatg gttttgtctt acggtttccg gatggtaaaa cagcagaaac
     1681 aggggtaggt tatgaagatt ggcattaccg ctatgttggg gtagagtctg caaaatatat
     1741 ggtcaaacat catttaacat tagaagaata cataacttta ttaaaggaga ataaccaatg
     1801 aggaaacgtt tttccttgct aaattttatt gttgttactt ttattttctt tttctttatt
     1861 ctttttccgc ttttaaacca taagggaaaa gtagatgcta attctaggca gagtgttacc
     1921 tacaccaaag aagaatttat acaaaaaatt gtgccagatg cgcaagatct aggaaagtcg
     1981 tacggtattc gtccttcatt tattattgca caggcggctt tggattctga tttcggagag
     2041 aaaatattag ctaataagta tcataatctg tttggtttgc ttgcagaacc aggaacgccc
     2101 tcaattacct taaatgatag tagtacagga aaaaaacagg aaaagcaatt tactcattat
     2161 aaatcttgga agtattcaat gtatgattac cttgctcata taaaatctgg agcgacaggc
     2221 aaaaaagatt catatactat aatggtgtct gttaaaaatc caaaaacttt agtgcaaaaa
     2281 ttacaagata gtggttttga taatgacaaa aagtacgcta aaaaaatgac ggaaatcatt
     2341 gatttgtatg atttaacaag atatgataag tgatattagc actctttgaa taagagtgct
     2401 aatttttgat gttttttcct tgactatctt ttaaaaagga gtataataaa atcatagtta
     2461 gcagtcgatg tgtaagagtg ctaatttcag aaagaccgag aggggtgatg tttttgtgat
     2521 tacacaacgt caaaatgata ttttgaattt aatcgttgag ttatttacac aaactcatga
     2581 accagtaggg tctaaagctt tacagagaac gattgattct agtagcgcca ctattcgtaa
     2641 tgatatggca aaactagaga agttggggtt attggaaaaa gcacacactt ctagtggacg
     2701 gatgccgagt ccagctggtt ttaagtattt tgtagagcac tcccttagac tagatagtat
     2761 tgatgagcaa gatatttatc atgttataaa agcttttgac tttgaagcat tcaaattaga
     2821 agacatgctt caaaaagcta gtcatatttt atcggagatg acgggctata catccgttat
     2881 tttagatgtg gagccagctc gtcaacgttt gacagggttt gatgttgttc agcttagcaa
     2941 ccatgatgct ctagcagtca tgactttaga tgaatctaag ccagtgacag tgcagtttgc
     3001 tattccaaga aactttttga cacgtgactt gatagcattt aaagctatcg tagaggagcg
     3061 cttgctggat ggtagtgtca tggatattca ttataagttg aggacagaaa ttcctcagat
     3121 cgttcagaag tattttgtaa ctacggataa tgttttacaa ctatttgatt atgttttttc
     3181 agagctgttt ttggaaacgg tatttgtagc tggcaaggta aattctttaa catattcaga
     3241 tctatcaacg tatcagtttt tggataatga gcagcaagta gcaatttctt tgaggcaagg
     3301 tctgaaagag ggtgagatgg cttctgtcca agtggcagat agtcaagaag ccgcacttgc
     3361 agatgtgtca gttttaactc acaaattttt gattccttac cgtggatttg gactcctgag
     3421 tttgattggt cctattgata tggattatcg taggtctgtt agtttggtaa acattatcgg
     3481 aaaggtattg gctgctaaat taggtgatta ttatcgatac ctaaattcta accattatga
     3541 agttcactga taatggcagt attcaataag ttatttaaaa ggaggcactc agtgtcagaa
     3601 gaaattaaaa aagacgattt acaggaagaa gtggaagcca ctgaaactga agagatagtt
     3661 gaggaagtta ttgaagaaat tcctgaaaaa tcagagttag aactcgcaaa cgagcgtgct
     3721 gatgagtttg aaaataaata cttgcgtgct catgcagaaa tgcagaatat acagcgtcgc
     3781 tctagtgaag aacgtcaaca acttcaacgc tatcgttctc aggatctggc aaaagcaatc
     3841 ttaccatcgc ttgacaatct agaacgtgcc ttggctgttg aagggttgac agatgatgtt
     3901 aaaaaaggtt tagaaatgac tcgtgatagc cttattcaag cactaaagga agaaggcgta
     3961 gaagaagtag aagtcgactc atttgaccac aactttcata tggcagttca gaccctccca
     4021 gccgatgatg aacatccagc cgatagcatt gcagaagtct ttcaaaaggg atataaattg
     4081 cacgagcgtc tgctaagacc agcgatggta gttgtctata actaaggtaa atgagttttc
     4141 gtttttgtcc gtaatgacag taaactagat agcaagttag aagctattca gcttgctgat
     4201 taaactatag tgattgctta gaattggaag taaaataatt cgagtgctta ctaagataaa
     4261 ttgaaataaa aagtaataaa gtattataaa ataagaggta ttaacatgtc taaaattatt
     4321 ggtattgact taggtacaac aaactcagca gtagcagttc ttgaagggac tgaatcaaaa
     4381 atcattgcta acccagaagg caatcgtaca actccttcag tagtatcatt caaaaatggt
     4441 gagattatcg tgggtgatgc tgcaaaacgt caagcggtaa caaatccaga tactgttatc
     4501 tctatcaaat caaagatggg aacttctgaa aaagtttctg caaatggtaa agaatatact
     4561 cctcaagaaa tttcagcaat gattcttcaa taccttaaag gttatgctga agactatctt
     4621 ggagaaaaag tagaaaaagc agttattact gttccagctt acttcaacga tgcacaacgt
     4681 caggcaacta aagacgctgg taaaattgca ggtcttgaag tagaacgtat cgttaacgaa
     4741 ccaacagcag ccgcacttgc ttatggtatg gacaagactg acaaggatga aaaaatctta
     4801 gtttttgacc ttggtggtgg tacatttgac gtatcaatcc ttgaattagg tgatggtgtc
     4861 ttcgacgttc ttgcaacagc aggtgataac aaacttggtg gtgacgactt tgaccagaaa
     4921 attattgatt tcttggtaga agaattcaag aaagaaaatg gtattgatct ttctcaagac
     4981 aaaatggctc ttcaacgctt gaaagatgct gctgaaaaag ctaaaaaaga cctttcaggt
     5041 gtaactcaaa ctcaaatttc attaccgttc atcactgctg gttctgctgg tcctcttcac
     5101 ttggagatga gcttatcacg tgctaaattt gacgatctca ctcgtgacct tgttgaacgt
     5161 acgaaaactc cagttcgtca agctctttca gatgcaggct tgtcattgtc agaaattgat
     5221 gaagttatcc tcgttggtgg atcaacacgt atcccagcag ttgttgaagc tgtaaaagct
     5281 gaaactggta aagaaccaaa taaatctgtt aaccctgatg aagtggttgc catgggtgct
     5341 gctatccaag gtggtgttat cactggggat gtgaaagacg ttgtacttct tgacgtaaca
     5401 ccattgtcac ttggtattga aacaatgggt ggtgtcttca ctaaattgat cgaccgcaac
     5461 acaactatcc caacatctaa atcacaagtc ttctcaacag cagcagacaa ccaaccagcc
     5521 gttgatatcc atgttcttca aggtgaacgc ccaatggcag cagataacaa aacactcggt
     5581 cgcttccaat tgactgatat cccagctgca cctcgtggaa tcccacaaat tgaagtaaca
     5641 tttgatatcg ataaaaatgg tattgtatct gttaaagcta aagatctcgg tactcaaaaa
     5701 gaacaacaca ttgttatcca atctaattca ggattaactg atgaagaaat tgataaaatg
     5761 atgaaagatg ctgaagcaaa tgctgaagca gatgcaaaac gtaaagaaga agttgatctt
     5821 aaaaatgaag ttgaccaagc catctttgca acagaaaaaa ctattaaaga aactgaaggt
     5881 aaaggctttg atacagaacg cgatgcagcg caatcagcac ttgatgagtt gaaaaaagct
     5941 caagaatcag gtaaccttga cgacatgaaa gctaaacttg aagctcttaa cgaaaaagca
     6001 caagctcttg cagttaaact ttacgaacaa gcagctgcag cacaacaagc agctcaaggg
     6061 gctgaaggtg cacaatcagc tgattcatca agcaagggtg atgatgttgt agatggcgaa
     6121 ttcactgaga aataattatt aatattgttc agattcattt gaatataagc atgaaaacta
     6181 tactagcata gtaaagttct tcgtgatagg ggttgctcaa taatctagat aagtttcaga
     6241 ttacataagc taatttcgct atcactaaat aaaaacatat taataataaa taggcggggc
     6301 gcctcgctcc gtctgtttta ttaagtgtca tatatatgtt aactatttag agctgtaact
     6361 gggcaagaat aattgttaat ctcttcaagt gtagtatatg aacaaaatat aaaggattag
     6421 ataatgaaca atacagaatt ttatgatcgt cttggcgttt caaaagatgc ttctcaggac
     6481 gaaataaaaa aagcttaccg taggatgtct aagaaatacc atccagatat taataaggaa
     6541 gcgggagcag aggaaaaata taaagaggtt caagaagctt atgaaacctt gagtgataca
     6601 caaaaacgtg ctgcctatga tcaatatggc gctgctgggg caaatggcgg ttttggtggc
     6661 ttcgatggtg gtggctttgg tggttttgaa gacatcttct caagcttttt tggtggagga
     6721 gggatgcgca atcctaatgc accacgtcaa ggagatgacc tacaatatcg tgttaattta
     6781 agttttgaag aagctatctt tggagctgaa aaagaagtat cttataatcg ggagtcatca
     6841 tgtcatactt gttcaggaag tggagctaag ccaggtacta gccctgtgac ttgtcaaaag
     6901 tgtcatggtt cgggtgtgat taatgttgat acacaaacac ctctaggaac aatgcgtcgc
     6961 caagtgactt gtgatgtttg tcagggtagt ggtcaagaaa tcaaagagaa atgtccaaca
     7021 tgtcatggaa ctggtcatga gaagaagacg cataaagtat cagttaaaat acctgcagga
     7081 gttgaaactg ggcaacaaat tcgtttaaca ggacaaggag aagctggctt caatggtggt
     7141 ccttatggtg atctctttgt tattattaac gttctcccaa gccaacagtt tgagcgtaat
     7201 ggatcaacaa tttattatac tttgaatatt ggctttgtcc aagctgcttt gggtgataca
     7261 attgatatac ctactgtcca tggagcggta gaaatgtcaa ttcctgctgg tactcagaca
     7321 ggaaaaacgt tccgtcttcg cggaaaaggt gcccctaaac ttcgaggggg tggacaaggt
     7381 gatcagcatg ttactgttaa catcgtaact cctactaaat tgaatgatgc tcaaaaagag
     7441 gcattgcacg cttttgcaga ggcaagtgga gataagatgg tacatccgaa gaagaaaggt
     7501 ttctttgata aagtcaaaga tgctttagat gttgattaaa aaataaaagt ctagaatcga
     7561 aagtattcta gacttttttg tagttattgc tagagggatt gcttgaacct aaattttaca
     7621 gtacaatgaa tctaatattg tagggggaca atttttatgg taaccaaagt tgaagagatt
     7681 cgctcatatt tgatagcttc tatacaaaat ggtaagttgg ctccaggaga tcgcctacca
     7741 tctataagac agttagctaa tcaattttcc tgtaacaaag atacagtgca acgagttttg
     7801 atggaattgc gttttgataa ttatatctat gcaaagccta ggtcaggcta ttacgtcttt
     7861 gattctcatc aagaggaagt tgaagaaggg gttagtttac caaactctga gattgcaaat
     7921 atagcttatg atgattttag attgtgtttg aatgagaccc ttattggtag ggaagattac
     7981 cttttcaatt attactatcg tcaagaaggt cttcttgatt taagtaaagc agtggctaaa
     8041 ttaatggaag aaacaggggt ctatgttccc cttgatgata ttgttattac ggctggtact
     8101 caacaggcat tatttatttt gacacaggtt acctttccaa atcgaaaatc tcgagtttta
     8161 atagaagaac cgacttatcc tcgtatgatt gaactaatca aaacacaaaa tttaccctat
     8221 gaaactattc ctcgaggtac tcatggaatt gattttcagc gcttagagga gattttccag
     8281 acacaatcaa ttaagttttt ttatgttata cctcgcatgc ataatccttt gggaacatcc
     8341 tataatccgg tagagatgaa aagattaata gagatggcag agaagtatga tgtttatatt
     8401 gtggaagatg actatatgtc tgattttgca agtcagtcac cattacatta ttatgatact
     8461 cacgggcgtg ttatttatct aaaatctttt tcaaaggcta ttttccctgc tttaagatta
     8521 gctgcgattt gtttaccaca agctttaaaa tcaacattta tggcttacaa gaagttgatg
     8581 gattatgata ctaatctgat tttacaaaaa gcattagcgc tttatattga aaatggcctt
     8641 tatgctaaga atagtcaata tttgaaatat cgttatcaga aagaccttgc aaattcaaaa
     8701 tctattttag ctgatcaccc taatctaccc ccatatagtt tacatcacga tagtgtatta
     8761 tttgattgtt cgaaactcga taacttcaaa atattacggc aatacggcga tactttggaa
     8821 aattattttt gtcaaaaatc gcatcaatct ctcttacaag taaaaaatga ttcctgctta
     8881 aagcagttct tgggatcgtt gtagtataat tgtaggggga cagtttaaaa tagatattat
     8941 agttttcata aatgaaaggt ggtaaaataa acccatgaca aggtataagg cacagatttc
     9001 ttatgatggt tcagcttttt caggcttcca aagacaacca aattgtagga cagttcaaga
     9061 agaaattgag agaactctta agcgcctaaa tagcgggaac gatgtgataa tctatggtgc
     9121 tggtcgcaca gatgctggtg ttcatgctta tggacaagtt attcattttg atttaccaca
     9181 ggcgcgtgat gtagaaaaat tacgctttgg tttagataca cagtgtccag acgatattga
     9241 tattgttaaa gtagagcaag tgtctgatga ttttcattgt cgatatgata aacacattaa
     9301 aacttatgag tttttagtag atatcggtag gccgaaaaat cctatgatga gaaactatgc
     9361 aacacattac ccttatcctg tcattattga gcttatgcaa gaagctatta aagatttagt
     9421 aggcacccat gactttacag gctttactgc ttcaggtacg agtgtagaaa ataaagtacg
     9481 tactattttt gatgcgaaaa ttcagtttga agcgtctaaa aatcttttaa tttttacctt
     9541 tacaggaaat ggttttttgt ataaacaggt gcgtaatatg gttggtaccc tgctaaaaat
     9601 tggtaatgga cgcatgccta ttagccaaat caaaacaatt ttacaggcta aaaatcggga
     9661 tttagcaggt ccaactgcgg caggaaatgg tttatatttg aaggagatta tttatgaaga
     9721 cgaggaatgt tttagcaatt tcaggaaatg atattttctc tggcggcggc ttgcatgcag
     9781 atttagcaac ttacgttgtt aataaattac atggttttgt ggcagtaaca tgcttaacag
     9841 caatgtcaga taaagggttt gaagtcattc ctatagaggc atctatacta aagcagcaac
     9901 tagaaagttt aaaagatgta gagtttgggt ctattaaact aggattgctt cccaatgtag
     9961 aaacagcaca agttgttttg gaatttgtaa aatcaaaaca agaatgtcca gtagtcttag
    10021 acccagtctt agtttgtaaa gaaaatcatg atttagaagt tagccaatta cgtgagcagc
    10081 taattgcgtt tttcccatat gctgatgtga ttactcctaa tttagtagag actcaattat
    10141 tgacgggtct ttcaattgaa aacttagatc aaatgaaaat tgctgctgag aaactttacg
    10201 atatgggggc taaacatgtt gttatcaaag ggggcaaccg tttaaatgct gaagaggcta
    10261 cagaccttta ctatgatggt gaacgttttg aaacctatgt ttttcctgta gtagatgcca
    10321 ataatacagg tgctggatgt acatttgcgt caagtattgc cagccaatta gctatgggta
    10381 aaaatgtaga agatgcagtc aaaatgtcta aaggtttcgt ctatcaagct attaaagcat
    10441 cggataagta cggagttgtc caacattttt aggagatttt aatgaggaaa gagaaaacta
    10501 gtcaattggt tcaattggca attgtaacag ctatatctat tgtattgggg atgtttataa
    10561 gtattccgac accaacaggt tttttgacat tactagatgc tggaatattc tttgctgcct
    10621 tttactttgg caaaaaagaa ggggctgtgg taggtgcctt agctggtttt ttaattgatt
    10681 tattgaaagg ttatcccaat tggatgttct ttagtttatt gattcatggg gcgcaagggt
    10741 atttagcggg tttaccaggt agaagacgtc ttttaggtct tattagtgca actcttgtta
    10801 tggtattagg ttatgctata gctagtggat tgatgtatgg ttggggagct gttttaccag
    10861 atattcctgg caatattatg caaaatatgg ttggtatggt cgttggtttt gctcttaata
    10921 agtctctaga aagagtgaag aaatgatact agattataat aagttaaagc aggaaactaa
    10981 agctattgta gttgatatta ttgagcgtag tgctcttaaa aaaggtcaaa tttttgtact
    11041 aggtctatca tctagtgagg tttctggtgg tttaattgga aaaaattcta gttctgaaat
    11101 tggtgaaatt atcgttgaag ttatcctcaa agagttacat tcacgcggca tctatttagc
    11161 agttcaaggt tgtgaacatg ttaatagggc gttggttgtt gaagcagaat tggctgaacg
    11221 tcaacagttg gaagtcgtta atgttgttcc taatctacat gctggcggta gtggtcaggt
    11281 tgcagctttt aagttgatga catcaccagt tgaggttgaa gaaattgtgg cacatgctgg
    11341 aattgatatt ggagatactt ctattggaat gcatatcaag agagtacaag ttcccttgat
    11401 tccaattagt cgtgagttgg gaggggcaca cgttacagca ctagctagtc gtcctaaatt
    11461 aattggcggt gctagagcag gatatacgag cgatccaatc cgtaaatttt aaaataaacc
    11521 tctgctatgc agaggtttat tgtttatctc aacgagagtg gttattggag aaattgtatg
    11581 ttggtaagtc aataccttct tcaagaatag ctttttgata aagtttgtaa aattcagcat
    11641 agatgtcaaa ctgagcaccg ttttgcgtga agatattaat acgataaaca aattgaccat
    11701 tagcattggt tgtaggacca aaaacagtag gctctccaac gatagcagga tgttttgaaa
    11761 catactcttc attgattttt gttacaatat cagaaatctg atcaaggttt gtatgaacaa
    11821 ataaaggaat atcaatttga gcacgcatat tgctacgaga cttattgctg acaattgtaa
    11881 tattacgatt aggaatgaag tgtaaggttc catcgaaatc atgaatttgt gtggtgcgta
    11941 tcccaacatt agtaacagtg cctgatacag caccaacatt aataatatca ccaacatcaa
    12001 attggttttc tagcagaata aaaaagccat tgacaacatc tgataagaag ccttgagcac
    12061 caagtccaat agcaacccca gcaataccag ccccagctaa taaactagaa attggaacgc
    12121 ccaatatact taaaatccaa tagaatagaa agaaataaag ggtatagttt aaaacattgt
    12181 gagaaagttt agctaatgtt ttttgacgag ctactttttg tctagaaaag gcgagtgagc
    12241 gattaacagt cttttcgaaa agataattga tgacttgcct aacgataacg aatgcaatga
    12301 ttaataataa gattgatatt agtttggtga agaaagtaaa taaaacttcc tctacattaa
    12361 gatggtca
//
LOCUS       DATROD010000045        12293 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.045, whole genome shotgun sequence.
ACCESSION   DATROD010000045 DATROD010000000
VERSION     DATROD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12293
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.045"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            216..1358
                     /gene="tgt"
                     /locus_tag="VBC74_001179"
     CDS             216..1358
                     /gene="tgt"
                     /locus_tag="VBC74_001179"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEO6942131.1"
                     /translation="MTDHPIKYRLIKQEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIAKAGGLHKFMNWDQAILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGAKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLNAHRRPHDQGLFGIVQGAGFEDLRRQSARDLVSMDFPGYSI
                     GGLAVGETHDEMNAVLDFTVPMLPNDKPRYLMGVGAPDSLIDAVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKFAEDFTPLDPNCDCYTCKNYTRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKDVRQAIMDDNLLEFRQDFMERYGYGTNNRNF"
     gene            1464..1775
                     /locus_tag="VBC74_001180"
     CDS             1464..1775
                     /locus_tag="VBC74_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942132.1"
                     /translation="MMQEYFGIGLDSSSRCYHYHTKLDIVALKCAVCQKYYACYKCHD
                     ALEEHCFVATKSDEMFPVLCGSCRQMLTLKEYKTGFCPYCRMLFNPNCQRHDSIYFSK
                     E"
     gene            1782..2321
                     /locus_tag="VBC74_001181"
     CDS             1782..2321
                     /locus_tag="VBC74_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEO6942133.1"
                     /translation="MFHTKSLIQMAMMATLLVVLGFIPALPLGFIPVPIVLQNLGVMM
                     AGVILGGRKGSLSILLFFLVGLVIPVFSGLRSTIPVLTGPTAGYVIAWLFVPLLISYG
                     VKLVNKKNFIAIFLIVWLAGVLFVDVAGAVWLASYMNIPLDKSLLQNLVFLPGDTIKA
                     IIAAVVAVKYKDSFFLTEK"
     gene            2460..3236
                     /locus_tag="VBC74_001182"
     CDS             2460..3236
                     /locus_tag="VBC74_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEO6942134.1"
                     /translation="MKLTTLGCWGGYPYKDGGTTSFLLTGENNFHLLIDAGSRSINEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLYHYRLLYPKDKWEPKVLPVYGHNEDDYEFS
                     KLTLPNVAQGIAYHTNGVEQVGPFNITFIKTVHPVVCYAMRITERETGQVLVFTGDSG
                     YFADLVPFTKGADLFLADVYLYEGNENHIAHLTSKEAGEIAYNAKVKKLVLTHVPQFP
                     GEGINPENHLEELRLQSEKYAGGISVDLAQPHKSWDLGKL"
     gene            3236..3742
                     /locus_tag="VBC74_001183"
     CDS             3236..3742
                     /locus_tag="VBC74_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEO6942135.1"
                     /translation="MQKFTQEEKEWFMTEALKEAKSSLEKEEIPIGCVIVKDGHIIGR
                     GHNAREEFNKAILHAEIMAINNANEKVGNWRLLDTTLFVTVEPCVMCSGAIGLARIPH
                     VIYGAKNTKFGAAGSLYDILSDSRLNHRVEVETGILEDQCAKIMQDFFRKGRQRKKEA
                     KLSEKTKI"
     gene            3777..3862
                     /gene="ffs"
                     /locus_tag="VBC74_001184"
     ncRNA           3777..3862
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VBC74_001184"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4014..5363
                     /locus_tag="VBC74_001185"
     CDS             4014..5363
                     /locus_tag="VBC74_001185"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEO6942136.1"
                     /translation="MTHITFDYSKVLGQFVGEHELDYLQPQVSAADAFLRQGTGPGSD
                     FLGWMDLPENYDKEEFSRIQKAAEKIKSDSEVLVVIGIGGSYLGAKAAIDFLNNHFAN
                     LQTAEERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKVEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVVRVEK
                     PRKNVTIPELTEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPTQDAY
                     TLGYTIYFFELAIGLSGYLNSVNPFDQPGVEAYKRNMFALLGKPGFEELSAELNARL"
     gene            5685..6212
                     /locus_tag="VBC74_001186"
     CDS             5685..6212
                     /locus_tag="VBC74_001186"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEO6942137.1"
                     /translation="MEKKLLRKEVLITLKSQPQAYKSEVDSKLLEAFIKTKAYQNSCV
                     IATYLSFDYEYNTQLLIKQALCDGKRVLVPKTYPKGKMIFVDYQKDNLRATPFGLLEP
                     VNDRAVEKASIDLIHVPGLIFNNKGFRIGYGAGYFDRYLSDFEGDTISTIYRCQRQDF
                     VEEKHDVAVKEVLCL"
     gene            6203..6880
                     /locus_tag="VBC74_001187"
     CDS             6203..6880
                     /locus_tag="VBC74_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEO6942138.1"
                     /translation="MSMKKFAKEYPTTVLLVGLTTLVFLLMQLTYGSQAESSQVIFQF
                     GGIQGDYLKAYPTNLWRLISPIFVHIGWEHFLLNGLALYFVGQMGESIWGSLRFLILY
                     ILSGLMGNIFTLFFTPHVVAAGASTSLFGVFSAIAIAGYFGKNPYLKQVGKSYQVMIL
                     LNLFFNIFTPGVSLAGHVGGLVGGVLVAIFLTKQNGSLLFKTWQSILALMIFIIVSIS
                     LIGLSLV"
     gene            7012..8055
                     /locus_tag="VBC74_001188"
     CDS             7012..8055
                     /locus_tag="VBC74_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO6942139.1"
                     /translation="MNRKIIGLTLLSLSVLTLTACGNRSDKSANKSDIKVAMVTNQGG
                     VDDKSFNQSAWEGLQKWGKKKGLTKGNGFDYFQSSNESDHANNLDTAASSGYNLIFGI
                     GFGIHDTIEKVSENNKDVKYVIVDDIIKGKENVASVTFADNEAAYLAGVAAAKTTKTK
                     TVGFIGGMEGVVVKRFEAGFKAGVKSIDPAIKVAVSYAGSFTDAAKGKTIAATQYATG
                     VDVIYQAAGGTGAGIFSEAKTENETRKESNKVWVIGVDRDQSQEGNYVSKDGKKANFV
                     LASTIKEVGKSLQSVAELTEKKQYPGGKVTVFGLKDSGVDIKEHQLSSEGSVAVKKAK
                     EGIVSGKIQVPMK"
     gene            complement(8146..9045)
                     /gene="galU"
                     /locus_tag="VBC74_001189"
     CDS             complement(8146..9045)
                     /gene="galU"
                     /locus_tag="VBC74_001189"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEO6942140.1"
                     /translation="MKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKNELLKLVDETTGIRLHFIRQ
                     SHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNNKVIPLTKQLINDFEATHASTIA
                     VMEVPHEDVSAYGVIAPQGEGVNGLYSVNTFVEKPSPEEAPSNLAIIGRYLLTPEIFN
                     ILETQKPGAGNEIQLTDAIDTLNKTQRVFARKFTGDRYDVGDKFGFMKTSIDYALQHP
                     QVKDDLKKYIIDLGKSLEKTSKK"
     gene            complement(9082..10098)
                     /locus_tag="VBC74_001190"
     CDS             complement(9082..10098)
                     /locus_tag="VBC74_001190"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO6942141.1"
                     /translation="MTKQKIAVLGPGSWGTALAQVLNDNGHEVRLWGNVVEQIEEINT
                     NHTNQRYFKDITLDSKIKAYTNLEEAINNVDSILFVVPTKVTRLVAKQVASLLKHKVV
                     IMHASKGLEPGTHERLSTILEEEISEQYRSDIVVVSGPSHAEEAIVRDITLITAASKD
                     IEAAKYVQKLFSNHYFRLYTNTDVVGVETAGALKNIIAVGAGALHGLGYGDNAKAAII
                     TRGLAEITRLGVQLGADPLTFSGLSGVGDLIVTGTSVHSRNWRAGDALGRGEKLEDIE
                     KNMGMVIEGISTTKVAYEIAQNLNVYMPITEAIYKSIYEGANIKDSILDMMSNEFRSE
                     NEWH"
     gene            10268..10597
                     /gene="rnpA"
                     /locus_tag="VBC74_001191"
     CDS             10268..10597
                     /gene="rnpA"
                     /locus_tag="VBC74_001191"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEO6942142.1"
                     /translation="MKKTYRVKSDKDFQMIFSRGKNVANRKFVIYYLEKEQKHFRVGI
                     SVSKKLGNAVVRNAVKRKIRHVLLSQKTALQDYDFVVIARKGVEELDYQALEKNLIHV
                     LKIAGLI"
     gene            10610..11425
                     /locus_tag="VBC74_001192"
     CDS             10610..11425
                     /locus_tag="VBC74_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEO6942143.1"
                     /translation="MKKKLKTFSLILLTGSLLVACGRGEVSSHSATLWEQIVYAFAKS
                     IQWLSFNHSIGLGIILFTLIIRAIMMPLYNMQMKSSQKMQEIQPRLKELQKKYPGKDP
                     DSRLKLNDEMQSMYKAEGVNPYASVLPLLIQLPVLWALFQALTRVSFLKVGTFLSLEL
                     SQPDPYYILPVLAALFTFLSTWLTNKAAVEKNIALTLMTYVMPFIILVTSFNFASGVV
                     LYWTVSNAFQVFQILLLNNPYKIIKVREEAVRVAHEKEQRVKRAKRKASKKRK"
     gene            11433..12254
                     /locus_tag="VBC74_001193"
     CDS             11433..12254
                     /locus_tag="VBC74_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEO6942144.1"
                     /translation="MVLFTGATVEEAIEKGLQELNISRLRAHIKVVSREKKGFWGFGK
                     KPAKVEIEGITDEVTDINESVALKNIKNVPSSVDVVEEYIEEVDETLEKEDVSQPELP
                     KIDDKNVVTTSEAIEKIDLLPNIEVAAAQVTKYVENIIYEMDLDATIETTTSKRQINL
                     QIETPEAGRIIGYHGKVLKSLQLLAQNYLHDRFSKSFSVSINVHDYVEHRIETLIDFS
                     KKIARRVLETNEPYHMDPMSNSERKTVHKTIATIEGVESYSEGNDPNRFVVVTKK"
BASE COUNT         4028 a         1931 c         2384 g         3950 t
ORIGIN      
        1 gcctcatttt taatttctga attcgtcata tctcctccat acataaaagt acaattcatt
       61 tcattataac acattcctta agatgagcat gacaagagta catttttttg ataaaatctt
      121 aatggctaaa aaagaaatta atacaaagta ttacatttag atattgatta gcactaaagc
      181 ccatgactgt ttttcatgga agcaaggaga aaacgatgac tgatcatcca ataaaatatc
      241 gattaattaa acaggaaaaa cacacaggag ccagacttgg tgaaataatc acaccacatg
      301 gtacatttcc gacaccaatg tttatgcctg ttgggacaca agcaactgtt aagacacaat
      361 ctcctgaaga actaaaagaa atgggttcag gaattattct atcaaatacc taccatttat
      421 ggttacgtcc aggtgatgaa ttgattgcta aagctggagg tttacataaa ttcatgaact
      481 gggatcaggc tattctaaca gacagtggag gctttcaggt ttattctttg gctgatagtc
      541 gaaacatcac tgaagaagga gtaaccttta aaaaccattt aaatggtgcg aaaatgtttt
      601 tatcaccaga aaaagctatt tcaattcaaa ataaccttgg ttctgatatt atgatgagtt
      661 ttgatgagtg tccacaattt taccagcctt atgattatgt aaaaaaatca attgagcgaa
      721 caagccgttg ggcagaacgt ggattaaatg cccaccgccg tcctcatgat caaggactat
      781 ttggtattgt tcaaggggca gggtttgagg atttacgtcg tcaatcagca cgagatttag
      841 tatcgatgga ttttccaggt tattcaattg gagggcttgc ggtcggagaa acacatgatg
      901 agatgaatgc agttcttgat tttactgttc caatgcttcc gaatgataag cctcgttatt
      961 tgatgggagt tggtgcacca gatagtttaa tagacgccgt tatccgagga gtagatatgt
     1021 ttgactgcgt cttacctact cgtattgctc gaaatgggac atgtatgaca agtcaaggtc
     1081 gtttagttgt taagaatgct aaatttgcag aagattttac gcctcttgat ccaaattgtg
     1141 attgctatac ttgtaaaaat tatacacgcg cttatatccg tcacttattg aaagcagatg
     1201 aaacttttgg tattcgtttg actagttacc ataatcttta tttccttgtc aatcttatga
     1261 aagatgtcag acaagctatt atggatgata atcttctaga gttccgtcaa gattttatgg
     1321 agcgctatgg ttatggcacg aataaccgta atttttaaaa aagttcctgg taaaacaggg
     1381 ctttttttga tctgtttgtt aaccgcgttt taaaaaaagt tgacaataga aaaacttttg
     1441 tataatatgt tataggagga atcatgatgc aagagtactt tggtattggt cttgatagta
     1501 gcagccgctg ttaccactat catactaaat tggatattgt cgctttaaaa tgtgctgttt
     1561 gtcaaaaata ttatgcttgt tataaatgtc atgatgcact cgaggagcat tgttttgtag
     1621 caacaaaatc tgatgaaatg tttcctgtct tatgcggttc ttgtcgtcag atgttgacat
     1681 taaaagagta taaaactggt ttttgtcctt attgtcgtat gctttttaat ccaaattgtc
     1741 aacgccatga ttctatttat ttttcgaagg agtaattttt aatgtttcac acaaaaagcc
     1801 taatccagat ggctatgatg gctactttat tggtggtact tggtttcatt cctgctctac
     1861 cacttggttt cattccggtc cctatcgtct tacaaaacct aggggttatg atggctggtg
     1921 ttattttggg cggaagaaaa gggagtttat ctattttgct cttctttctt gtcggtttag
     1981 ttattcctgt attctcaggt ttacgttcga ctatacctgt attgacagga ccaacagctg
     2041 gttatgttat tgcttggttg tttgttcctt tgcttatctc atatggtgta aaattagtaa
     2101 ataaaaagaa ttttatagct attttcctta ttgtatggct agcaggggtc ctttttgtag
     2161 atgtagcagg agcagtttgg ctggcatctt atatgaacat cccattagat aaatcacttc
     2221 ttcaaaactt agtatttctc ccaggagata caataaaagc cattatagct gctgttgttg
     2281 ctgttaaata taaagatagt tttttcttga ctgaaaaata atcaagattg ctatatttta
     2341 gatggactag tagatctaaa aatttagatt tactagtctt ttttgtgcat aaaatctaaa
     2401 acagttattc ttaacaaaat ttggtataat agcattaaat cagtaaggag gggattatca
     2461 tgaaattaac aacgttaggt tgttggggtg gttatcctta taaagatgga ggaaccacaa
     2521 gttttctatt aactggagaa aataattttc acttattaat agatgcggga agtcgttcaa
     2581 tcaatgagtt agaaaaagaa ataagcccgt tggatttgga tgcagttatc atcagtcatt
     2641 atcatccaga tcatgtagca gatttgggtg tgttgtatca ttatcgtttg ctatacccta
     2701 aagataaatg ggaacccaaa gtattgccag tttatggaca taatgaggat gattatgagt
     2761 tttctaaatt gacattacct aacgttgctc aaggaattgc ttatcatacc aatggagttg
     2821 agcaagttgg tcccttcaat attacgttta tcaaaacagt ccacccagtc gtctgctatg
     2881 ccatgaggat tacagaaaga gaaactggtc aagtattagt ctttacagga gattctgggt
     2941 attttgctga ccttgtacct tttacaaaag gcgcagattt atttttagca gacgtttatc
     3001 tttacgaggg aaatgaaaat catattgcac atttgactag taaagaagca ggtgagattg
     3061 cttataacgc taaagttaag aaattagttt taacacatgt tccgcagttt ccgggagaag
     3121 gaataaaccc cgaaaatcat ttggaagaac ttcgactaca gtctgaaaag tatgcaggtg
     3181 gcatctctgt tgatttagct cagccacata aatcttggga cttaggaaaa ctataatgca
     3241 gaaatttacc caagaagaaa aagaatggtt tatgacagaa gctctaaagg aggcaaaatc
     3301 ctccttagag aaagaagaaa ttcctatagg ttgtgtcatt gtaaaagatg gtcatattat
     3361 tggaagagga cataatgctc gtgaagagtt taataaagct attttacatg ctgaaatcat
     3421 ggctattaac aatgcaaatg agaaggtagg gaattggcgc ttattagata ctactttatt
     3481 tgttactgta gaaccttgtg taatgtgtag tggagctatt ggcttagcta gaattcccca
     3541 cgttatatat ggcgctaaga atacgaaatt tggagcagcg ggaagtctct atgatatttt
     3601 aagtgatagc cgacttaatc atagagttga agttgaaacc ggcattttag aagatcaatg
     3661 cgctaaaata atgcaggatt tctttcgaaa aggacgacaa cgcaagaaag aagctaaact
     3721 aagtgaaaaa actaagattt gatagcttgc aataaaatat tagttttgtt ataatatctt
     3781 tggagcaaca tttactcgtg aagtgggtca ggggaggaat ccagcagccc taagcgatgc
     3841 taaatgtgtg ctctttttat tttcaaaagt caaagtgttt ttggaaataa ttatgaaagt
     3901 ttcataaaaa ttggtagcgt ttcaaaaaat atgaaaatac agttaaaaaa ccttgaaaaa
     3961 aaactcacaa aagagtaaga tgatagtgaa acataattgt taggagaaat aaaatgacac
     4021 atattacatt tgactattca aaagtcttgg gtcaatttgt aggcgaacac gagttagact
     4081 acctacaacc acaagtaagt gcagcagatg ctttccttcg tcaagggact ggtcctggat
     4141 cagattttct cggatggatg gacctcccag aaaactatga caaagaagaa ttttctcgca
     4201 ttcaaaaagc cgctgaaaag attaaatcag atagcgaagt actcgtggtt attggtattg
     4261 gtggttcgta ccttggtgca aaagcagcaa ttgacttttt gaataatcat tttgctaatt
     4321 tgcaaaccgc agaagaacgt aaagcgcctc agattcttta tgctggaaat tctatttcat
     4381 ctacttacct tgccgattta gttgaatacg tccaagataa agaattctca gtaaatgtca
     4441 tttcaaaatc aggtacaaca actgaaccag cgattgcttt ccgtgtattt aaagaacttc
     4501 tagttaaaaa gtacggtcaa gaagaagcta ataaacgtat ctacgctaca actgataaag
     4561 ttaaaggtgc cgtaaaagtt gaagcagatg ctaacaattg ggaaacattt gtagttcctg
     4621 ataatgttgg tggtcgtttc tcagtactga cagctgtagg tcttcttcca attgctgctt
     4681 caggagcaga tattactgct cttatggaag gcgcaaatgc tgctcgtaaa gatttatctt
     4741 cagataaaat ctctgaaaac attgcttatc agtacgctgc agttcgtaat gttctttatc
     4801 gcaaaggtta tatcacagag attttagcta actacgaacc atcattacaa tactttggtg
     4861 aatggtggaa acaattagca ggtgaatcag aaggtaaaga tcaaaaaggt atctatccta
     4921 catcagcaaa cttctcaact gatcttcact cacttggtca atttattcaa gaaggttacc
     4981 gtaacctctt tgagacagtt gttcgcgttg agaaacctcg taaaaatgtg actattcctg
     5041 aattaaccga agatttagat ggtcttggtt atcttcaagg aaaagatgta gattttgtta
     5101 ataaaaaagc aacagatggt gtgcttcttg ctcatacaga tggtggggtt ccaaatatgt
     5161 ttgtaacgct tcctacacaa gacgcttaca ctcttggtta cactatttac ttctttgagt
     5221 tagcaattgg cctttcaggt tatcttaact cagtaaatcc atttgatcaa ccgggggtag
     5281 aagcatataa acgtaatatg tttgctcttc taggtaaacc tggattcgaa gagcttagcg
     5341 ctgaattgaa tgcacgtctt taaaaatatc taactaaaaa gttcctcaaa gagaggaact
     5401 ttttagtttt gttgtaggat aactaaaaac ccactagcac tggtagcgat agtgggttgg
     5461 aaaaagggtt gagaaaagtt attaactttt ctcaggagat ttatgaaaaa ggaaaataat
     5521 ttaggataat attttcctaa cggctaggat aaaccgctta ttttaggatg atgctagtat
     5581 acagtagaaa ccttaaataa accttaaaaa ttcatacttt atcattttat tttcccctat
     5641 actatttttg tataaatggt caatattaat ggtatcatag atatatggaa aagaaattat
     5701 taagaaaaga agttttgata accttaaaat ctcaacctca agcttacaaa tcagaagttg
     5761 actctaaact tttagaggct tttataaaaa ctaaagctta tcaaaatagt tgtgtaattg
     5821 ctacttacct atcttttgac tacgaataca atacacaact tcttattaaa caagctttat
     5881 gtgatggcaa acgtgtcctt gttcccaaaa cttatccaaa aggtaaaatg atttttgtgg
     5941 actatcaaaa agataacttg agggcaacac cgtttggctt attagaacct gttaatgata
     6001 gagctgttga aaaagctagt atagacttaa ttcatgtgcc cggacttatc tttaacaata
     6061 aaggttttcg tataggttat ggtgcaggtt attttgatcg ctatttgagt gattttgaag
     6121 gtgatactat tagtactatt tataggtgtc agagacagga ttttgtagaa gagaagcatg
     6181 atgtagcggt taaggaggta ttatgtctat gaagaagttc gctaaagagt atccgacgac
     6241 agtactcttg gtcggtctga caacccttgt ttttttattg atgcaactaa cttatggaag
     6301 tcaagcagag tctagccaag ttatttttca atttggtgga atacaaggtg attatctaaa
     6361 agcatatcca acaaatctat ggagactaat aagtcctatc tttgttcata ttggttggga
     6421 acatttttta ttaaatggtt tagctctgta ttttgtggga caaatggggg aaagtatttg
     6481 gggaagtttg agatttctaa ttctctatat tttatctggc ttaatgggta atatttttac
     6541 gctatttttt actccacacg tggtggcagc aggagcctca acgtctttat ttggtgtttt
     6601 ttctgctata gcaatcgcag gttattttgg aaaaaatcct tatttaaaac aagttggaaa
     6661 atcatatcaa gtgatgatct tgttaaattt attttttaat atcttcacac ccggagtgag
     6721 tttagcaggc catgttggtg gtttagttgg tggagtattg gtggctattt ttttgacaaa
     6781 acaaaatggt tctttattat ttaaaacttg gcaaagtatt ttagctttaa tgatatttat
     6841 tattgtatcg atcagtttaa taggtctatc gttggtataa ttctacggct atagtctcta
     6901 aaaccaaact ttctttggga aaaatagaat aaagtttgct aaatgatttt tttatgttat
     6961 gatagaaatc tgtttttatg tgactataac ttattaggag gttcattctc ggtgaacaga
     7021 aaaattattg gcttaactct actatcttta tctgtactaa cattaacagc atgtggtaat
     7081 cgttcagata agtctgcaaa taaatcagat atcaaagttg cgatggtaac taatcaaggt
     7141 ggagtagacg ataaatcgtt taatcaatca gcttgggaag ggctacaaaa gtggggaaaa
     7201 aagaaggggt taacaaaagg taatggcttt gattatttcc agtcatctaa tgaatcagat
     7261 catgctaata accttgatac ggcagcctct agtggctata atttaatttt tggtattgga
     7321 tttggtattc atgatactat tgaaaaagta tcagaaaata ataaagatgt taaatacgtt
     7381 attgtagatg atatcattaa aggtaaagaa aatgtagcta gtgttacttt tgcagacaat
     7441 gaagctgctt acttagctgg agttgctgca gctaagacaa ctaagacaaa aacagttggt
     7501 tttatcggag ggatggaagg cgtcgttgtt aagcgatttg aagcaggatt taaggctgga
     7561 gtaaaatcaa ttgatccggc tattaaagtt gcggtatctt atgcaggctc atttacagat
     7621 gcagctaaag gtaaaacgat tgctgccaca cagtatgcaa caggtgttga tgttatttac
     7681 caggctgctg gtggtacagg agcaggaatt tttagtgagg ctaaaactga aaatgagacc
     7741 cgcaaggaat ctaacaaagt ctgggtaatt ggtgttgaca gagatcaaag tcaagaaggc
     7801 aattatgttt caaaagatgg taagaaagca aactttgttc tggcatctac tataaaagag
     7861 gtggggaaat ctctccaatc tgtagcagaa ttaacagaga aaaaacaata tccaggaggg
     7921 aaagtaacag tttttggtct aaaggatagt ggtgtagata ttaaagagca tcaattgtct
     7981 agtgagggat ctgttgctgt caaaaaagct aaagagggga ttgtttcagg gaaaatacaa
     8041 gttcctatga aatgataaaa gtgtggagga acaataagtt cttcgtcaag tacaataaaa
     8101 aagtttccta aataggaaac ttttttattg tacttgactc atattttatt tcttagatgt
     8161 tttttctaga gattttccta aatcaatgat atatttcttc aagtcatctt taacttgtgg
     8221 gtgttgaaga gcataatcaa tagaagtttt cataaaacca aacttgtcac caacgtcata
     8281 acggtcccct gtaaattttc tagcaaaaac acgttgcgtt ttatttaaag tatcgatagc
     8341 atcggttagt tgaatctcat ttcctgcacc aggtttttga gtttctaaaa tattaaaaat
     8401 ttctggagtt aacaagtagc gaccaatgat tgccaaatta cttggagcct cttcgggaga
     8461 aggtttttca acgaaagtgt taacgctata tagaccatta actccttctc cttgtggagc
     8521 aataacacca taagcagaaa catcttcatg tggaacttcc ataacagcaa ttgttgatgc
     8581 atgagtagct tcaaagtcat taattaattg tttggtgaga ggaataactt tattattggt
     8641 aatatccatt agatcatcac ctagcataac aacaaaaggt tcattaccaa cgaaggcttt
     8701 tgcttgtagc acagcatctc caagcccacg aggatgactt tgtcgaataa aatggagtcg
     8761 aatgccagtt gtttcatcaa caagtttgag aagttcgttt ttccctttct cttttaggtt
     8821 atattctaat tcaaaatttg aatcaaagtg gtcttctata gaacgttttg atttaccagt
     8881 tacaactaaa atatcttcaa ttccagattt tagtgcttct tcaacaataa attgaatagt
     8941 tggtttatca acgattggaa gcatttcttt agctaaagct ttagtagcag gtaaaaaacg
     9001 tgttcctaaa ccagcggcag ggataactgc ttttctgact ttcatatata gctcctttaa
     9061 atagtattaa tataaacgat attagtgcca ttcattctct gaacgaaatt cattggacat
     9121 catatctaaa atgctatctt tgatattagc cccttcatat attgatttat aaatagcttc
     9181 tgtaatgggc atgtaaacat ttaagttttg ggcgatttcg taagcaacct tagttgttga
     9241 gataccttca ataaccatcc ccatattttt ttcaatatct tcaagtttct caccacgtcc
     9301 taaagcatct cccgctctcc agttgcgaga atgaacagat gtaccagtca caattaaatc
     9361 tccaactccg gataaaccac tgaaagtgag agggtcggcc cctaattgta caccaaggcg
     9421 tgttatctca gctaaaccac gcgtaataat agctgcttta gcattatcac cataaccaag
     9481 accgtgtagt gctccagcac caacagcaat aatatttttt agagcaccag ctgtttctac
     9541 tcctacgaca tcagtgttag tataaagtct aaaataatga ttactaaaca atttttggac
     9601 atacttagct gcttcaatat cttttgaagc agcagtaatc aaagtaatat cacgaacgat
     9661 ggcttcttca gcatgactgg gacctgaaac aacaacgata tcactacgat attgttcaga
     9721 gatttcttct tctaaaattg tagaaagtcg ttcatgagtg ccaggttcta gccccttaga
     9781 agcatgcata attacaactt tatgctttaa aagactagcc acttgtttag caacaagtct
     9841 tgtaacttta gttgggacga cgaatagaat gctgtcaaca ttattaatcg cttcctcaag
     9901 attagtgtag gcttttattt tggaatctaa ggtaatatct ttgaagtaac gttgattagt
     9961 atggttagta ttaatctctt ctatttgttc aaccacgttt ccccataagc gtacctcatg
    10021 tccattgtca tttaatactt gtgcaagagc agtcccccat gaaccaggtc caagaacggc
    10081 aattttttgt tttgtcatct tattccttct ttctaataat aaggcatata gcgctaacat
    10141 tatatcatac tctaatacac tttgtcttgt ttagtctgtt gtcgatgata gtagcattag
    10201 aaaagaattg agtcttttag gttatttatg gtataataaa accaatttag ataaatggag
    10261 cactattttg aaaaaaacct atcgggtaaa aagtgataag gattttcaaa tgattttcag
    10321 cagaggtaaa aatgttgcta atcgaaaatt tgttatctac tatttagaaa aagaacaaaa
    10381 acactttcgt gtgggaatct ctgttagtaa gaaattagga aatgctgttg ttcgtaacgc
    10441 tgttaaaaga aaaattaggc atgttttact ctctcagaaa acagcgctgc aagattatga
    10501 ttttgtcgtg attgcgcgta aaggtgtaga ggaattagac tatcaagcat tagaaaaaaa
    10561 tcttatacac gttttaaaaa tagcaggact catctagaaa ggtataaaat tgaagaaaaa
    10621 acttaaaaca tttagcttaa tattgctgac aggttcttta ttggttgctt gcggacgagg
    10681 agaagtatct agtcattcag ctacattatg ggagcaaata gtttatgcat ttgctaaatc
    10741 aatacaatgg ctctccttta accattcaat tggtttggga ataatcttat tcactctaat
    10801 aatcagagcg attatgatgc cgctttataa tatgcaaatg aaatccagcc aaaagatgca
    10861 ggaaattcag ccaaggctca aggaattaca aaaaaaatat ccagggaaag atcctgatag
    10921 ccgtttaaag ctaaatgatg aaatgcaatc tatgtataaa gctgagggag ttaacccata
    10981 tgcaagtgta ttacctcttc taattcagtt acctgtccta tgggctcttt tccaagcttt
    11041 gactagggtt tctttcttaa aagtaggaac gtttttgagc ctagaattat cacagccgga
    11101 cccatattat attttacctg tactggcggc cttatttacc tttttatcaa cttggttaac
    11161 taacaaagca gctgttgaaa aaaatattgc tttaactctt atgacttatg ttatgccgtt
    11221 tatcatattg gtaacaagtt ttaactttgc tagtggtgtt gttttatact ggacggtgtc
    11281 taatgctttt caagtatttc aaattctatt attgaacaac ccatacaaga ttataaaggt
    11341 tcgagaagaa gcagtaagag tggcacacga aaaagaacaa agagttaaac gtgctaagag
    11401 aaaagcaagt aagaaaagaa agtaggaaaa acatggtatt atttacagga gcgactgtag
    11461 aagaagctat tgaaaaagga ctacaagagt taaacatttc acgattgagg gcacatataa
    11521 aagttgtatc acgtgagaaa aaaggttttt ggggctttgg taaaaagcca gcaaaagttg
    11581 aaatagaagg tatcaccgac gaagtaactg atattaatga atcggtagcg ttaaaaaata
    11641 ttaagaatgt accgtctagc gtagatgtgg tggaagaata tatagaggag gttgatgaaa
    11701 cgcttgaaaa agaagacgta tcacaaccag aacttccaaa aattgatgat aaaaacgttg
    11761 taactacaag tgaagctatt gaaaaaattg acctacttcc taatattgaa gtagcagctg
    11821 ctcaggtgac taaatacgtt gaaaatatca tctatgagat ggatttagat gcaacaatag
    11881 aaactacgac aagtaagcga caaataaatc tccaaataga aacgccagaa gctgggcgca
    11941 tcattggtta tcatgggaaa gtattaaaat cgttgcagtt gttagcacaa aattatcttc
    12001 atgaccgctt ttctaagagt ttttcagtat ctattaatgt ccatgactac gttgagcatc
    12061 gtatagaaac tttaattgat ttttctaaaa aaatagctag acgtgtttta gagactaatg
    12121 agccttacca tatggatcct atgagtaaca gtgagcgaaa aactgttcat aaaacaattg
    12181 caactataga gggtgttgaa agttattctg aaggtaacga tcctaataga tttgtagtag
    12241 ttactaagaa gtaaaaagag gtttcacctc tttttttctt gcgcccgatc tac
//
LOCUS       DATROD010000046        12029 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.046, whole genome shotgun sequence.
ACCESSION   DATROD010000046 DATROD010000000
VERSION     DATROD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12029
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.046"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(37..603)
                     /locus_tag="VBC74_001194"
     CDS             complement(37..603)
                     /locus_tag="VBC74_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000596040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6942145.1"
                     /translation="MIKRPIHLSHDFLAEVIDKEAITLDATMGNGNDTVFLAKSSKKV
                     YAFDIQEEAIAKTKAKLTEQGISNAELILDGHENLEQYVHTPLRAAIFNLGYLPSADK
                     TVITKPHTTIKAIKNVLDILEVGGRLSLMVYYGHDGGKSERDAVIAFVEQLPQNNFAT
                     MLYQPLNQVNTPPFLIMVEKLQSYENEV"
     gene            complement(596..1531)
                     /locus_tag="VBC74_001195"
     CDS             complement(596..1531)
                     /locus_tag="VBC74_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747785.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEO6942146.1"
                     /translation="MKKRYRAINDYYRELFGEKIFKLPIDAGFDCPNRDGTVARGGCT
                     FCTVSGSGDAIVAPEAPIREQFYKEIDFMHRKWPEVNKYLVYFQNFTNTHAKLEIIKE
                     RYEQAINEPGVIGINIGTRPDCLPDETIYYLAELSERMHVTLELGLQTTYEATSALIN
                     RAHSYDLYKKTVKRVRELAPKVEIVSHLINGLPGETHDMMVENVRRCVTDNDIQGIKL
                     HLLHLMINTRMQRDYHEGRLRLLSQEDYISIICDQLEIIPKHIVIHRITGDAPRHMLI
                     GPMWSLNKWEVLNAIDKEMEKRQSYQGCKVEVKHD"
     gene            complement(1624..2265)
                     /locus_tag="VBC74_001196"
     CDS             complement(1624..2265)
                     /locus_tag="VBC74_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO6942147.1"
                     /translation="MLSRQNSKLIQAFIAIILFFSLGLVIKYWPDTVIAFDQTIQESV
                     RGQLPNLSTRFFKLITVVGNTVSQIAIAIMSVTFCYLKKWYPQARFIAVNAIISGICI
                     LSLKLIFQRVRPTLTHLVFAGGYSFPSGHSMGTFMIFGSSIILLQYYMPKSIWKLLCQ
                     GTLGLLIFLIGLSRIYLGVHFPTDVLAGFILAYGILNLTAYIFLSKDWLKKVK"
     gene            complement(2246..2830)
                     /locus_tag="VBC74_001197"
     CDS             complement(2246..2830)
                     /locus_tag="VBC74_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6942148.1"
                     /translation="MTNTRKMVTIAILSALSFVLMMVSFPLIPGAEFLKVDFSILPML
                     VAFILFDLKSSYGVLLLRSLLKVILANRGPETFIGLPMNMVALALFLASFAIFWKNRE
                     SAKDFIKASLFGTVSLTVSMVALNYVFAIPLYAIFANFDIRTFIGVGNYLLTMVIPFN
                     IVEGILISIVFYLTYVACLPILERYKKTNVISSK"
     regulatory      complement(2900..3045)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(3114..3713)
                     /locus_tag="VBC74_001198"
     CDS             complement(3114..3713)
                     /locus_tag="VBC74_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEO6942149.1"
                     /translation="MIRRYSANFLAILGIILVSSGIYWGWYNINQAHQADLTSQHIVK
                     VLDKSITHQVKGSENGELPVKKLDKTDYLGTLDIPNLKLHLPVAANYSFEQLSKTPTR
                     YYGSYLTNNMVICAHNFPYHFDALKNVDMGTDVYFTTTTGQIYHYKISNREIIEPTAI
                     EKVYKTATSDNDWDLSLFTCTKAGVARVLVRCQLIDVKN"
     gene            complement(3706..4377)
                     /locus_tag="VBC74_001199"
     CDS             complement(3706..4377)
                     /locus_tag="VBC74_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942150.1"
                     /translation="MIYKKILKITLLLLFSLSTQLVSADTNDQMKTGSITIQNKYNNQ
                     GIAGGNLLVYQVAQAKDVDGNQVFTLTTPFQGIGIKDDDLTQVNLDSNQAKYVNLLTK
                     AVHKTQPLQTFDNLPAEGIVANNLPQGIYLFIQTKTAQGYELMSPFILSIPKDGKYDI
                     TAFEKMSPLNAKPKKEETITPTVTHQTKGKLPFTGQVWWPIPILIMSGLLCLIIALKW
                     RRRRD"
     gene            complement(4367..5242)
                     /locus_tag="VBC74_001200"
     CDS             complement(4367..5242)
                     /locus_tag="VBC74_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEO6942151.1"
                     /translation="MKKIRQHLVSILLVTIIAVGVGLMAYPSLANYWNSFHQSRAIMD
                     YQDRVTHMDENDYKKIINRAKEYNKQFKTSGMKWHMTSQERLDYNSQLAIDKTGNMGY
                     ISIPKINIKLPLYHGTSEKVLQTSIGHLEGSSLPIGGDSTHSILSGHRGLPSSRLFSD
                     LDKLKVGDHWTVSILNETYTYQVDQIRTVKPDDLRDLQIVKGKDYQTLVTCTPYGVNT
                     HRLLVRGHRVPNDNGNALVVAEAIQIEPIYIAPFIAIFLTLILLLISLEVTRRARQRK
                     KILKQAMRKEENNDL"
     gene            complement(5280..6788)
                     /locus_tag="VBC74_001201"
     CDS             complement(5280..6788)
                     /locus_tag="VBC74_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEO6942152.1"
                     /translation="MKKKMIQSLLVASLAFGMAVSPVTPIAFAAETGTITVQDTQKGA
                     TYKAYKVFDAEIDNANVSDSNKDGASYLIPQGKEAEYKASTDFNSLFTTTTNGGRTYV
                     TKKDTASANEIATWAKSISANTTPVSTVTESNNDGTEVINVSQYGYYYVSSTVNNGAV
                     IMVTSVTPNATIHEKNTDATWGDGGGKTVDQKTYSVGDTVKYTITYKNAVNYHGTEKV
                     YQYVIKDTMPSASVVDLNEGSYEVTITDGSGNITTLTQGSEKATGKYNLLEENNNFTI
                     TIPWAATNTPTGNTQNGANDDFFYKGINTITVTYTGVLKSGAKPGSADLPENTNIATI
                     NPNTSNDDPGQKVTVRDGQITIKKIDGSTKASLQGAIFVLKNATGQFLNFNDTNNVEW
                     GTEANATEYTTGADGIITITGLKEGTYYLVEKKAPLGYNLLDNSQKVILGDGATDTTN
                     SDNLLVNPTVENNKGTELPSTGGIGTTIFYIIGAILVIGAGIVLVARRRLRS"
     gene            complement(6829..11133)
                     /locus_tag="VBC74_001202"
     CDS             complement(6829..11133)
                     /locus_tag="VBC74_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HEO6942153.1"
                     /translation="MLKKCQTFIIESLKKKKHPKEWKIIMWSLMILTTFLTTYFLILP
                     AITVEETKTDDVGITLENKNSSQVTSSTSSSQSSVEQSKPQTPASSVTETSSSEEAAY
                     REEPLMFRGADYTVTVTLTKEAKIPKNADLKVTELKDNSATFKDYKKKALTEVAKQDS
                     EIKNFKLYDITIESNGKEAEPQAPVKVEVNYDKPLEASDENLKVVHFKDDGQTEVLKS
                     KDTAETKNTSSDVAFKTDSFSIYAIVQEDNTEVPRLTYHFQNNDGTDYDFLTASGMQV
                     HHQIIKDGESLGEVGIPTIKAGEHFNGWYTYDPTTGKYGDPVKFGEPITVTETKEICV
                     RPFMSKVATVTLYDDSAGKSILERYQVPLDSSGNGTADLSSFKVSPPTSTLLFVGWSK
                     TQNGAPLSESEIQALPVSSDISLYPVFKESYGVEFNTGDLSTGVTYIAPRRVLTGQPA
                     STIKPNDPTRPGYTFAGWYTAASGGAAFDFNQVLTKDTTLYAHWSPAQTTYTINYWQQ
                     SATDNKNATDAQKTYEYAGQVTRSGLSLSNQTLTQQDINDKLPTGFKVNNTRTETSVM
                     IKDDGSSVVNVYYDRKLITIKFAKYGGYSLPEYYYSYNWSSDADTYTGLYGTTLAANG
                     YQWKTGAWGYLANVGNNQVGTYGMSYLGEFILPNDTVDSDVIKLFPKGNIVQTYRFFK
                     QGLDGTYSLADTGGGAGADEFTFTEKYLGFNVKYYQRLYPDNYLFDQYASQTSAGVKV
                     PISDEYYDRYGAYHKDYLNLVVWYERNSYKIKYLDPLDNTELPNFPVKDVLYEQNLSS
                     YAPDTTTVQPKPSRPGYVWDGKWYKDQAQTQVFDFNTTMPPHDVKVYAGWQKVTYRVN
                     IDPNGGRLSKTDDTYLDLHYGDRIPDYTDITRDYIQDPSGTYYYKYDSRDKDPDSTKD
                     AYYTTDTSLSNVDTTTKYKYVKDAYKLVGWYYVNPDGSIRPYNFSGAVTQDINLRAIW
                     RKAGDYHIIYSNDAVGTDGKPALDASGQQLQTSNEPTDPDSYDDGSHSALLRRPTMPD
                     GYRFRGWWYNGKIYNPYDSIDIDAHLADANKNITIKPVIIPVGDIKLEDTSIKYNGNG
                     GTRVENGNVVTQVETPRMELNSTTTIPENQYFTRTGYNLIGWHHDKDLADTGRVEFTA
                     GQSIGIDNNPDATNTLYAVWQPKEYTVRVSKTVVGLDEDKTKDFLFNPSETLQQENFP
                     LRDGQTKEFKVPYGTSISIDEQAYDEFKVSESITEKNLATGEADKTYDATGLQSLTVS
                     GDVDISFTNTRIKQKVRLQKVNVENDNNFLAGAVFDIYESDANGNKASHPMYSGLVTN
                     DKGLLLVDANNYLSLPVGKYYLTETKAPPGYLLPKNDMSVLVISTGVTFEQNGNNATP
                     IKENLVDGSTVYTFKITNSKGTELPSTGGIGTHIYILVGLALALPSGLILYYRKKI"
     gene            complement(11153..11722)
                     /gene="lepB"
                     /locus_tag="VBC74_001203"
     CDS             complement(11153..11722)
                     /gene="lepB"
                     /locus_tag="VBC74_001203"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO6942154.1"
                     /translation="MKRQISSDKLSQELDRVTYQKRFWSVIKNTIYILMAVASIAILI
                     AVLWLPVLRIYGHSMNKTLSAGDVVFTVKGSNFKTGDVVAFYYNNKVLVKRVIAESGD
                     WVNIDSQGDVYVNQHKLKEPYVIHKALGNSNIKYPYQVPDKKIFVLGDNRKTSIDSRS
                     TSVGDVSEEQIVGKISFRIWPLGKISSIN"
     gene            complement(11706..11978)
                     /locus_tag="VBC74_001204"
     CDS             complement(11706..11978)
                     /locus_tag="VBC74_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942155.1"
                     /translation="MEDLKKLKRKDLLEIMLEQQLLIEKQEKEIQQLKEALDDKKIKI
                     SKAGSIAEASLMLSGIFETAQLAADTYLENVKNLVDKRGVDNEKTD"
BASE COUNT         3664 a         2313 c         1931 g         4121 t
ORIGIN      
        1 tagcaattat catttttgct aattgcctaa agatagttac acctcatttt catatgattg
       61 taacttttct accataatta aaaagggggg cgtattaact tgattgagtg gttgataaag
      121 cattgtagcg aaattattct gtggtaattg ttccacgaat gctatcaccg catctctctc
      181 actttttcca ccatcatgac cataataaac cataagtgat agacgtccac ctacttctaa
      241 aatgtctaaa acgtttttga ttgccttgat agttgtgtgt ggttttgtaa taactgtttt
      301 atccgctgag ggtaagtatc ccagattaaa tatagccgct cttaaaggtg tatgaacata
      361 ctgttccaaa ttttcatgcc catccaagat aagttcagcg ttagagatcc cttgctccgt
      421 gagctttgct ttcgtttttg ctattgcttc ttcttgaata tcaaatgcat aaactttttt
      481 agaacttttc gctaaaaaaa cagtatcatt cccatttccc atagttgcat caagcgttat
      541 tgcttcttta tcaataactt ctgctaaaaa atcatgagaa agatgaattg gtcttttaat
      601 catgttttac ctcaacttta catccttgat atgactgtct tttctccatt tctttgtcaa
      661 tggcattaag cacttcccat ttattaaggc tccacatcgg tccaattaac atatgacgtg
      721 gcgcatcacc agtaattcgg tgaataacaa tatgttttgg aataatctcc agttggtcac
      781 aaataatcga aatatagtct tcttgactta acaaacgtaa acgaccttca tggtaatctc
      841 tctgcattct tgtattaatc ataagatgca gaaggtgtaa ttttataccc tgaatatcat
      901 tatccgtaac acatcgacga acattttcca ccatcatatc atgtgtctcc cctgggagac
      961 catttattag gtgagaaacg atttctactt taggagctaa ttctcgtact ctcttaacag
     1021 tttttttgta aaggtcatat gaatgtgctc tatttattaa tgcagaagtt gcttcataag
     1081 ttgtctgaag gcctaattct aaagtcacat gcattctttc agaaagttca gcgagatagt
     1141 atatagtttc atcaggtaag caatccggcc ttgttccgat gttgatcccg ataactcctg
     1201 gctcattaat agcctgttcg taacgctctt taattatctc taacttagca tgggtattgg
     1261 taaaattttg aaaatagact aagtatttat taacctcagg ccactttcta tgcatgaaat
     1321 caatttcttt atagaattgt tcacgaatag gagcttctgg agcaactata gcatcccctg
     1381 aaccagaaac tgtgcaaaaa gtgcaccctc ctctagcaac tgttccatct ctgttaggac
     1441 agtcaaaacc agcatctata ggtaatttaa atattttttc tccaaagagt tctcgataat
     1501 aatcattaat cgcacgataa cgttttttca taggataatt gtatcacaat tttaactaaa
     1561 ataacctcac tactacaata aaactaaaaa agattggaac gtcagttagt cccaatcttt
     1621 tatttacttc actttcttta accaatcctt ggataaaaag atatacgcag ttagattcaa
     1681 aataccataa gcaagtataa aaccagctaa aacatctgtc ggaaaatgaa cccctaggta
     1741 aatacgagat aacccaatta aaaaaatgag caaacccaat gtaccttggc acaacagttt
     1801 ccatatactc ttaggcatat agtactgcaa taaaataata ctactcccaa atatcataaa
     1861 tgttcccatc gagtgcccac tgggaaacga atagccacct gcaaatacta aatgggttaa
     1921 agttggtctc actctttgaa aaataagttt taaagaaagt atacatatac cagagataat
     1981 agcatttact gcgataaatc tagcttgagg ataccacttc ttaaggtaac agaaagtgac
     2041 gctcataatc gcaatagcta tctggcttac agtattacca accacagtga ttaacttaaa
     2101 aaatcttgta gaaagatttg gcaactgtcc tctaacactt tcttgaatag tttggtcaaa
     2161 tgcgattaca gtgtcgggcc aatatttgat gaccaatcct aaactgaaaa ataagataat
     2221 agcaataaat gcttgaataa gtttactatt ttgacgagat aacattagtc tttttatatc
     2281 tttctaatat tggcaaacaa gccacgtaag ttagatagaa aacaatcgaa attaaaattc
     2341 cctcaacgat attaaatgga ataaccattg ttaaaaggta attgcctaca ccaataaatg
     2401 ttctgatatc aaagttagca aatatagcat acaaaggaat cgcaaagaca tagttgagag
     2461 ctaccataga tacagtcaag ctaactgtac caaataaact agctttaata aaatcttttg
     2521 cactctctct atttttccag aaaatagcga aacttgctaa aaatagagct agagcaacca
     2581 tattcatcgg taaaccgata aaggtttctg gaccacgatt agcaagtata acttttaaaa
     2641 gtgatcttaa taagagtaca ccataacttg atttcaaatc aaataaaata aaagcaacta
     2701 acatcggaag gattgaaaaa tcaaccttta aaaattctgc tcctggtatt aatggaaatg
     2761 aaaccatcat caatacaaaa gataaggcag aaagaatggc gattgtcacc attttacgtg
     2821 tatttgtcat aaaaaaattc ctccaattta aataaattga aagaagctcc aaaggtaagc
     2881 gtaggtacgc gaaaaaaacc tttgtcttct cccatccaga ctttactgtc ggttgtggaa
     2941 tctcaccaca tcagctttcg ctcgcggact gatgcttcac aactgacaaa taagttggaa
     3001 gcgattaccg ccggtcggga attacaccct gccctgaaga cacctatagc ataacaaaaa
     3061 aaacttgcaa ttgcaagttt tttaatcact aattagtagt agattgtata atattaattt
     3121 ttaacatcaa ttaattgaca gcgcactaat actctagcta ctcctgcctt tgtacaagta
     3181 aacaagctta agtcccaatc attgtctgat gtggcagttt tataaacttt ttcaatcgct
     3241 gttggttcaa taatttctct attactgatt ttgtagtgat agatttgccc tgttgtagtt
     3301 gtaaaataaa catccgttcc catatctaca ttttttaaag catcaaaatg ataaggaaaa
     3361 ttatgcgcac aaatcaccat gttattagtt aaataagaac cataatacct tgtaggcgtc
     3421 ttagacagtt gttcaaaact ataattagca gctaccggta aatgcagttt taagttcgga
     3481 atatccagag ttcccaagta atctgtttta tccaactttt ttacaggtaa ttctccattt
     3541 tctgaaccct ttacttgatg cgtaatagat ttatcaagca ccttgacaat atgctgagaa
     3601 gttaaatcag cttgatgcgc ctgattaata ttataccaac cccaatagat tccagaactt
     3661 accagaataa ttccgagtat agctaaaaaa tttgctgaat atcttctaat cacgtcttct
     3721 tctccatttt aaagctatta ttaaacacag aagtcctgac ataattagta taggtattgg
     3781 ccaccatact tgtccagtaa acggaagctt tccctttgtc tgatgtgtta ctgtaggagt
     3841 aattgtctct tcttttttag gtttagcatt taaagggctc attttctcaa atgctgtaat
     3901 atcgtacttc ccatccttag gtattgatag tataaaggga gacattagtt cataaccttg
     3961 agccgtttta gtctgaataa atagataaat cccttgagga agattgttcg caacaatacc
     4021 ttcagccggt aaattatcaa acgtttgtaa aggttgagtt ttatgaacag cttttgttag
     4081 tagattgacg tatttggctt ggttactatc aaggtttact tgtgttagat catcgtcttt
     4141 tattccaata ccttgaaatg gggtagttag agtaaaaact tgattaccat caacatcttt
     4201 agcttgtgct acttggtaaa caagtaaatt accgccagcg ataccttgat tattatactt
     4261 attttgtata gtaatagaac ccgttttcat ctgatcattg gtatcagcag acacaagttg
     4321 agtacttaga ctaaataata agagaagagt tatctttagg atctttttat aaatcattgt
     4381 tctcttcctt tctcattgct tgttttaaaa ttttcttacg ttgacgtgct ctcctagtta
     4441 cttctaaaga gattaaaagt aaaatcaaag taaggaaaat agcgataaat ggtgcgatat
     4501 aaataggctc tatttgtatt gcctctgcta ctaccaaagc gttaccatta tcgtttggta
     4561 cacgatgtcc tctcactagt aaccgatggg tattaacgcc atatggtgta cacgtcacca
     4621 aagtttggta gtctttacct ttaacaattt gtaaatccct caaatcatcc ggtttaactg
     4681 ttctgatttg atccacttga taagtatatg tttcatttaa gatactgact gtccagtggt
     4741 ctccaacttt taacttatcc aaatcagaaa aaagccttga agagggtaaa cctctatgtc
     4801 ctgataaaat agaatgagtt gagtctcctc caattggaag actacttcct tctaaatgac
     4861 caatagaagt ttgaagcact ttttcacttg taccatgata aagtggtaat tttatgttta
     4921 tctttggaat tgaaatataa cccatattac ccgttttatc gatagccagt tgtgaattat
     4981 aatccaaacg ctcttggcta gtcatgtgcc acttcattcc tgaagtttta aattgcttat
     5041 tatattcttt ggctcggtta ataatttttt tataatcgtt ttcatccata tgcgttacgc
     5101 ggtcttggta atccataatc gctcgagatt ggtgaaatga attccaataa ttagcaagtg
     5161 aaggataagc cattaagcct acccccactg caattatagt gacaagcaaa atggatacta
     5221 aatgttgtct tatttttttc atatatttta aatctgtacc actttgctag cccatcttat
     5281 taagaacgta aacgacgacg agcaacaagc acgatacctg ctcctattac taaaattgca
     5341 cctataatgt agaaaattgt tgtaccaata ccacctgttg aaggcaactc agtacctttg
     5401 ttattttcaa cagttgggtt aactaaaagg ttatctgaat tagtcgtatc agtggctcca
     5461 tctcctaaaa taaccttctg agagttatct aacaaattgt aacctaaggg agcctttttc
     5521 tcaactagat agtatgtacc ttctttcaag cctgtaatgg taattatacc atctgctcct
     5581 gttgtatatt ctgttgcatt agcttctgtg ccccattcaa cgttatttgt atcgttaaag
     5641 tttagaaatt gacccgtagc attctttaaa acaaatatag caccttgtaa tgaagctttt
     5701 gtggaaccat caattttttt tatagtaatt tgaccatccc tcactgttac tttttgacct
     5761 gggtcatcat tgctagtatt ggggttgatg gtcgcaatgt ttgtattttc tggtaaatca
     5821 gctgaacctg gtttagctcc actctttaat actcctgtat aagtgactgt gattgtattt
     5881 attcccttat aaaaaaagtc atcattagct ccattttgag tatttccggt tggagtattg
     5941 gtagctgccc acggaatagt aatcgtgaaa ttattatttt cctctaacag gttatacttc
     6001 ccagttgctt tttccgaacc ttgagttaga gttgtaatat tccctgatcc atcagtaata
     6061 gttacttcat aagacccttc gttcaaatca actacagaag cagatggcat agtatccttt
     6121 ataacatatt gatacacttt ttctgtacca tgataattga ctgcattctt ataagtaata
     6181 gtatatttga ctgtatcacc aaccgagtac gttttttgat ctacagtttt tccaccacca
     6241 tctccccatg tcgcatcagt attcttttca tgaatagtag catttggagt tacagatgta
     6301 accataatta cagctccatt attaacagtg ctagaaacat aataatatcc atattgggaa
     6361 acattaataa cctcagtacc atcattattt gactcagtaa cagtggaaac tggtgtagta
     6421 ttagctgata tagatttagc ccatgtcgca atctcatttg ctgacgcagt atctttttta
     6481 gttacatatg ttctccctcc attagtagtt gtcgtaaaaa gagaattaaa atcagttgaa
     6541 gctttatact cagcttcttt accttgagga attaaataag aagctccatc tttattcgaa
     6601 tcagatacat ttgcattatc tatttctgca tcaaaaactt tatatgcttt ataggttgcg
     6661 cctttttgag tatcttgaac tgtaattgtc cctgtctcag cggcaaaagc tatcggcgta
     6721 actggtgata cagccatacc aaatgctaaa ctcgccacta acagcgattg aatcattttc
     6781 tttttcattg aaatctttct cctaaaatca tattgatgaa tgattaattc atattttttt
     6841 tcgatagtat aatattaatc ctgatggtag agctaaagct aaaccaacta ggatataaat
     6901 gtgtgttcca atacctccag tactaggcaa ttctgttcct ttactgttag taattttaaa
     6961 agtatatact gtacttccat ccactaaatt ctcttttatt ggtgtcgcat tattaccatt
     7021 ttgttcaaag gtaactcccg tagaaatcac taatactgac atatcatttt taggtagtag
     7081 gtaccctgga ggggcctttg tctctgttag gtagtatttt cctactggca aactgaggta
     7141 gttattagca tccactaata acaagccttt atcgtttgtc accagccctg aatacatagg
     7201 atgtgaagct ttattcccat tagcatctga ttcataaata tcaaaaactg cacctgctaa
     7261 aaaattatta tcattttcga cattaacttt ctgtagtcgt actttttgct tgatacgtgt
     7321 attggtaaag ctaatatcta cgtctcctga aactgtcagg gattgtaagc cggtagcatc
     7381 ataagtttta tcagcttcac cagttgctag atttttttct gtaattgact cagatacttt
     7441 aaattcatcg taggcttgtt catctattga tatagaagtt ccataaggta ctttaaattc
     7501 cttagtctga ccatctctca gcggaaaatt ctcttgttgc aacgtttcac ttggattaaa
     7561 caagaagtct ttcgtcttat cttcatctag tccaacgaca gttttactta ctctgacggt
     7621 gtattcttta ggttgccaaa cagcatataa ggtatttgtt gcatcagggt tgttatcaat
     7681 acctattgat tgacctgctg taaattccac acgtcctgta tcagctaaat ccttatcatg
     7741 atgccaacca ataaggttgt aacctgtcct tgtaaagtat tggttttcag gaattgtagt
     7801 tgtgctattc aactccatac gcggtgtctc cacttgtgtt accacattac cattttctac
     7861 tctagtacca ccgttaccat tgtatttgat ggaggtatct tctaatttga tatctcctac
     7921 tggaataatg acaggtttta tggtgatatt tttattagca tctgctaaat gggcgtcaat
     7981 atcaatggaa tcatatgggt tataaatttt accattgtac caccagccac ggaaacgata
     8041 gccatctggc attgtcggac gtctcagtaa ggctgaatgg gagccatcgt cataggaatc
     8101 agggtcagta ggctcattac ttgtttgtaa ttgctgacca gaagcatcca atgctggctt
     8161 tccatctgta ccaacagcat cattgctgta tataatatga taatctccag cctttcgcca
     8221 aatagctctt aaattgatat cttgagttac agcacctgaa aagttataag gtctaatact
     8281 accatctgga ttaacataat accaaccgac taatttgtaa gcgtctttta cgtacttgta
     8341 cttagttgtt gtatcaacat ttgagagact agtatctgtc gtataatagg catctttagt
     8401 tgagtcggga tctttatctc gtgaatcata cttataataa tatgtacctg aaggatcttg
     8461 gatataatcc cttgtaatat ctgtataatc cggaatacga tcaccataat gcaaatctaa
     8521 ataggtatca tctgtttttg ataatcggcc tccgtttgga tcaatattga cacgatatgt
     8581 taccttttgc caacctgcat agactttaac atcatgagga ggcatagtcg tgttaaagtc
     8641 aaatacttgt gtttgtgctt ggtctttata ccatttacca tcccaaacat accctggtcg
     8701 actaggttta ggttgaacag ttgtcgtatc gggggcataa gaggacaaat tttgctcata
     8761 tagaacatcc tttactggaa aattaggaag ctctgtatta tcaagcggat ctaaatattt
     8821 aatcttgtat gaattacgtt cataccatac cactaagttc aaataatctt tgtggtaggc
     8881 accatatcta tcatagtatt catctgaaat aggaactttt actcctgcac tcgtttgact
     8941 tgcgtactga tcaaaaaggt aattatcagg atataaacgt tgataatatt taacattaaa
     9001 ccctagatat ttttctgtaa aggtaaattc atctgcccca gcacctccac cggtatctgc
     9061 taaagaataa gtgccatcca aaccttgttt gaagaaccga taagtttgaa ctatattccc
     9121 ttttggaaag agtttgataa catctgaatc aacggtatca ttaggtaaaa tgaactcacc
     9181 caaataactc atcccatagg ttccaacttg gttatttcca acattggcta aataacccca
     9241 tgcacccgtc ttccattgat aaccattagc ggccaaggtt gtaccgtaaa gtcctgtgta
     9301 agtatcagca tcagagctcc aattatacga atagtaatat tctggcaagg aatatccgcc
     9361 atattttgca aattttattg tgatgagttt tctatcataa taaacattaa caacacttga
     9421 accatcgtct tttatcatga cagaagtttc tgtcctcgta ttattaactt taaagccagt
     9481 cggtagttta tcattaatat cttgttgtgt tagcgtctga ttagataaag ataggcctga
     9541 tcgtgttact tgccctgcgt actcatatgt cttttgcgca tcagtagcat ttttattatc
     9601 cgttgctgat tgttgccagt agtttatcgt gtaggttgtt tgtgcgggcg accaatgtgc
     9661 atatagcgtc gtatccttgg tcaagacttg attaaaatca aaggctgccc caccactagc
     9721 agctgtgtac caccctgcaa aagtataacc tggcctcgtt ggatcattag gcttaattgt
     9781 cgaagcaggt tggcctgtta acacacgacg aggtgcaata taggtaactc ctgttgataa
     9841 gtcgcctgtg ttgaattcaa caccgtacga ttctttaaag acaggataaa gacttatgtc
     9901 tgaagataca ggtaatgctt gaatttctga ttcagaaagt ggagctccat tttgagtttt
     9961 agaccaacct acaaataaca atgttgaagt gggtggtgaa actttaaatg aacttaaatc
    10021 cgcggttccg tttcctgaac tatccaatgg tacttgataa cgctccaaaa tacttttacc
    10081 agcagaatca tcatataaag ttactgtcgc cactttactc ataaatggac gaacacaaat
    10141 ttcttttgtc tcagttacag ttattggctc accaaattta acagggtcac catactttcc
    10201 agtagtagga tcataggtat accaaccatt aaaatgctct cctgctttaa tcgtcggaat
    10261 cccaacttct cctagagatt ctccatcttt tataatttga tgatgaactt gcatacctga
    10321 agctgtcagg aaatcataat cagttccgtc attattttga aaatggtaag ttaacctagg
    10381 aacttctgta ttatcctctt gaacaattgc ataaatggag aatgaatctg ttttaaaagc
    10441 aacatcactg ctagtgttct tagtttctgc agtatcttta gattttaata cttctgtttg
    10501 accatcatct ttaaagtgaa caactttaag gttttcatct gaagcttcta atggcttatc
    10561 atagttaacc tctactttta ctggagcttg gggttctgct tctttaccat ttgactcaat
    10621 agtaatgtca tagagtttga aatttttgat ttcactatct tgtttagcaa cctctgtcaa
    10681 tgcttttttc ttataatctt taaaagtagc tgaattgtct tttaattccg tcacctttaa
    10741 atcagcattt ttaggaatct tagcttcttt ggtcaaagtc actgttacag tatagtctgc
    10801 acctctaaac atcaatggtt cttcacgata agcagcttcc tcagaagatg atgtttctgt
    10861 tacactagaa gcaggagtct ggggcttgct ctgctcaaca cttgattgag aactagatgt
    10921 tgatgaagtt acctggctag aatttttatt ttctaaagta atccccacat catctgtctt
    10981 agtttcttca actgttattg ctggtagaat taaaaaataa gtcgttaaaa aagttgttag
    11041 gatcatcaat gaccacatga taattttcca ctctttaggg tgttttttct tttttaatga
    11101 ttcgattata aaagtttgac acttctttag catttttgca tcctccctaa ccttaattga
    11161 tactactaat cttacctaga ggccatattc tgaaagaaat tttacctaca atttgttctt
    11221 ctgaaacatc tcctacagaa gtacttcgag aatcaattga agtttttcgg ttgtctccta
    11281 atacaaaaat ttttttatca ggtacttgat atgggtattt tatattacta ttaccgagtg
    11341 ctttatgaat aacatatggt tctttcaact tatgttgatt cacgtaaaca tccccttgag
    11401 aatcaatatt aacccagtct cctgactctg caataacccg cttgactagg accttattat
    11461 tgtagtaaaa cgcgacaacg tctccagttt taaaatttga accttttact gtaaagacta
    11521 catcacctgc acttaaagtc ttattcattg aatgtccgta gattcttaat acaggcaacc
    11581 ataaaaccgc aattaaaatg gctattgagg caaccgccat caagatgtat atggtatttt
    11641 taatgacact ccaaaagcgt ttctgataag ttacgcgatc cagttcttga gataatttat
    11701 ctgaactaat ctgtcttttc attgtctacg cctctcttat ctactaaatt ctttacgttt
    11761 tctaaatagg tatctgcagc taactgagca gtctcaaaaa taccactaag cattaaggaa
    11821 gcttcggcaa tagaaccagc tttgctaatt ttgatttttt tatcatctag cgcttcttta
    11881 agttgctgaa tctctttctc ttgtttttca ataagaagtt gctgttctaa cataatttct
    11941 agcaagtctt ttctttttaa tttttttaaa tcttccatcg cgattacttc cttaactgaa
    12001 ccttaaatta tcgtttagat attatatca
//
LOCUS       DATROD010000047        11961 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.047, whole genome shotgun sequence.
ACCESSION   DATROD010000047 DATROD010000000
VERSION     DATROD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11961
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.047"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            100..2601
                     /locus_tag="VBC74_001205"
     CDS             100..2601
                     /locus_tag="VBC74_001205"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEO6942156.1"
                     /translation="MTFYNHKEIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVAEYKRQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFKLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKASA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQVLTASGESISYVPWPSYDESKLVENEIEIVVQIKGKVKAKLVVAKDLSREEL
                     QEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVVK"
     gene            complement(2691..3299)
                     /locus_tag="VBC74_001206"
     CDS             complement(2691..3299)
                     /locus_tag="VBC74_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome assembly protein"
                     /protein_id="HEO6942157.1"
                     /translation="MLHSKKIHSLSLIAVLSLATYTSLQPNHVAAEQSQKTSTVHMSQ
                     KTIEHKLKVADKEAAPLYAKIDHIQRHIEVKKAKDLKVIELYINKDINQLEKQNKRLL
                     TKFYTSIDNQTWDSTSEVKKLIDKTTLSTNEKDRLKLYFEQRAYLETRLNDRYQKFDN
                     SIENQNKELKILTSKIEKIYQKHGITKEVLKTYYAKKTVRAD"
     gene            complement(3392..4780)
                     /locus_tag="VBC74_001207"
     CDS             complement(3392..4780)
                     /locus_tag="VBC74_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6942158.1"
                     /translation="MKKKSLISNLILINSITLFLGFYLIYFSVNYVIKDYVHQMTRNS
                     MQANFTILDSIYNNKKIPDENMVNQDSIFVWSYYAIFDQHYRMKYGTDNHKKESHDII
                     RYLTQKRLWQISKEKDGMFITIKKKTYYVMTKDYSGILVDGSIKKAPKAQSQLFHVIN
                     FSDITYTQHLITKINHFLIVILVLTYIPMLFIMRKTFTGIRESIQSVQTYISSLWKNQ
                     GNHQSSQKEIVFSDFDPLLLESQEMANRIYQAEESQRNFFQNASHELRTPLMSIQGYT
                     EGVQEGIIDAELAHSVILQESKKMKQLVDDIILLSKLDSNLSDQKDKFSLNELLNSII
                     AYFKPLANKQKISITYRPDKHEKLLKGNEELIQRAINNILSNALRYAVSHIEISYTNQ
                     KLTISNDGPAISKEDLPYIFDRFYKGHGGQTGIGLAMTKEIIKQHHGNIIAESDSTST
                     TFTIYFNCHKIA"
     gene            complement(4777..5462)
                     /locus_tag="VBC74_001208"
                     /pseudo
     CDS             complement(4777..5462)
                     /locus_tag="VBC74_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161341.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(5695..10410)
                     /locus_tag="VBC74_001209"
     CDS             complement(5695..10410)
                     /locus_tag="VBC74_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEO6942159.1"
                     /translation="MNTKQRFSIRKYKLGAVSVLLGTLFFLGGITNVAADSVINKPSD
                     IAVEQQVKDSPTSIANETPTNNTSSALASTAQDNLVTKANNNSPTETQPVTEPHSQAT
                     ETFSPAANQPVESTQEVSKTPLTKQNLAVKPTPAISKETPQNIDSNKIITVPKVWNTG
                     YKGEGTVVAIIDSGLDTNHDALQLNDSTKAKYQNEQQMNAAKAKAGINYGKWYNNKVI
                     FGHNYVDVNTELKEVKSTSHGMHVTSIATANPSKKDTNELIYGVAPEAQVMFMRVFSD
                     EKRGTGPALYVKAIEDAVKLGADSINLSLGGANGSLVNADDRLIKALEMARLAGVSVV
                     IAAGNDGTFGSGASKPSALYPDYGLVGSPSTAREAISVASYNNTTLVNKVFNIIGLEN
                     NKNLNNGLAAYADPKVSDKTFEVGKQYDYVFVGKGNDNDYKDKTLNGKIALIERGDIT
                     FTKKVVNAINHGAVGAIIFNNKAGEANLTMSLDPEASAIPAIFTQKEFGDVLAKNNYK
                     IVFNNIKNKQANPNAGVLSDFSSWGLTADGQLKPDLSAPGGSIYAAINDNEYDMMSGT
                     SMASPHVAGATALVKQYLLKEHPELKKGDIERTVKYLLMSTAKAHLNKDTGAYTSPRQ
                     QGAGIIDVAAAVQTGLYLTGGENNYGSVTLGNIKDKISFDVTVHNINKVAKDLHYTTY
                     LNTDQVKDGFVTLAPQQLGTFTGKTIRIEPGQTKTITIDIDVSKYHDMLKKVMPNGYF
                     LEGYVRFTDPVDGGEVLSIPYVGFKGEFQNLEVLEKSIYKLVANKEKGFYFQPKQTNE
                     VPGSEDYTALMTTSSEPIYSTDGTSPIQLKALGSYKSIDGKWILQLEQKGQPHLAISP
                     NDDQNQDAVALKGVFLRNFNNLRAKVYRADDVNLQKPLWVSAPQAGDKNYYSGNTENP
                     KSTFLYDTEWKGTTTDGIPLEDGKYKYVLTYYSDVPGSKPQQMVFDITLDRQAPTLTT
                     ATYDKDRRIFKARPAVEHGESGIFREQVFYLKKDKDGHYNSVLRQKGEDGILVEDNKV
                     FIKQEKDGSFILPKEVNDFSHVYYTVEDYAGNLVSAKLEDLINIGNKNGLVNVKVFSP
                     ELNSNVDIDFSYSVKDDKGNVIKKQHHGKDLNLLKLPFGTYTFDLFLYDEERANLISP
                     QSVTVTISEKDSLKDVLFKVNLLKKAALLVEFDKLLPKGATVQLVTKTNTVVDLPKAT
                     YSPTDYGKNIPVGDYRLNVTLPSGYSTLENLDDLLVSVKEGQVNLTKLTLINKAPLIN
                     ALAEQTDIISQPVFYNAGTHLKNNYLANLEKAQTLIKNRVEQTSIDNAIAALRESRQA
                     LNGKETDTSLLAKAILAETEIKGNYQFVNASPLSQSTYINQVQLAKNLLQKPNVTQSE
                     VDKALENLDIAKNQLNGHETDYSGLHHMIIKANVLKQTSSKYQNASQFAKENYNNLIK
                     KAELLLSNRQATQAQVEELLNQIKATEQELDGRDRVSSAENYSQSLNDNDSLNTTPIN
                     PPNQPQALIFKKGMTKESEVAQKRVLRVTSQTDNQKIKTNKLPKTGESTPKITYTILL
                     FSLSMLGLATIKLKSIKRE"
     gene            10733..11425
                     /locus_tag="VBC74_001210"
     CDS             10733..11425
                     /locus_tag="VBC74_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEO6942160.1"
                     /translation="MEKGFKEGVKDALPTALGYISIGLAFGIVASASDLSAIEVGLMS
                     ALVYGGSAQFAMCALLLAKADLMTITMTVFLVNLRNMLMSLHATTIFKSAHLMNQLAI
                     GTLITDESYGVLLGEALHHKVVSPSWMHGNNVMSYLTWVISTIIGTLLGSTIPNPEMF
                     GLDFALVAMFIGLFVFQLFGMLSDGKRLVVYVLASVGLSYFLLSTFLSGALSVLLATV
                     VGCSVGVVLDDK"
     gene            11415..11738
                     /locus_tag="VBC74_001211"
     CDS             11415..11738
                     /locus_tag="VBC74_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEO6942161.1"
                     /translation="MINSTVFLAILLGFVVSWVPRVAPFIFVKYKGLPNSLVKFLKFL
                     PLSILFALTFSSFFSVKLGQLPTLKWLDLLAGIPTLVVTIRYRNLFYTVLTGIVTMAL
                     LRLLF"
BASE COUNT         3443 a         2088 c         2296 g         4134 t
ORIGIN      
        1 tggggagaga acctgctgtt gagggtattt tcaccttttt tattgctgtt tttcgtgtta
       61 taataagagg actatacttt tttgacatgg agaagtgcga tgacattcta taatcacaaa
      121 gaaatcgaac ccaaatggca agctttttgg gcagataatc acacctttaa aacaggaaca
      181 gacgcatcaa aacctaagtt ttatgcccta gatatgttcc catacccatc tggagctggt
      241 ctccacgttg gtcacccaga aggctataca gcaacggata ttctcagccg tttcaaacgt
      301 gcccaaggtc acaatgtcct ccacccaatg ggttgggatg cttttggact tcctgctgag
      361 cagtacgcta tggatactgg taatgaccca gctgagttta cagctgaaaa catcgctaac
      421 ttcaaacgcc agattaatgc tcttggtttc tcatacgact gggatcgtga agttaacaca
      481 actgacccta actactacaa gtggacacag tggatcttta ccaagcttta tgaaaaaggt
      541 ctagcttacg aagctgaagt tccagttaac tgggttgaag aattgggaac agctatcgcc
      601 aacgaagagg ttcttccaga tggtacatca gaacgtggtg gctacccagt tgtcagaaaa
      661 ccaatgcgcc aatggatgct caaaatcact gcctatgcag agcgcttgct agaagacttg
      721 gaagaagttg actggccaga atcaatcaaa gacatgcaac gcaactggat tggtaaatca
      781 actggtgcta atgtaacctt caaggttaag gatacagata aggacttcac tgtctttaca
      841 acacgtcctg acaccctttt tggtgcaact tacgcagttc ttgctcctga gcatgccttg
      901 gttgatgcta ttacaacagc tgaccaagca gaagccgttg ccgaatacaa acgtcaagca
      961 agtctcaaat cagaccttgc tcgtacagac cttgccaaag aaaaaacagg tgtctggaca
     1021 ggtgcctatg ccattaaccc agttaatggt aaagaaattc cagtttggat tgcggactat
     1081 gtccttgcca gctacggtac aggtgctatc atggctgttc cagcccacga tgaacgtgac
     1141 tgggaattcg caaaacaatt taagctagac atcattccag ttcttgaagg tggtaatgtt
     1201 gaagaggctg cctttacaga agacggtctt cacatcaact cagacttcct agatggtctc
     1261 gataaagcgt cagctattgc caaaatggtc gaatggcttg aagcagaagg tgttggtaat
     1321 gagaaagtca cttaccgtct ccgcgactgg ctcttcagcc gtcaacgtta ctggggtgag
     1381 ccaatcccaa tcatccactg ggaagatggt acatcaacag cggttcctga gagtgaattg
     1441 ccattggttc ttccagtaac taaggatatc cgtccttcag gtactggtga gagcccactt
     1501 gctaacttga cagactggtt ggaagtgact cgtgaagatg gtgttaaagg tcgtcgtgag
     1561 actaacacca tgccacaatg ggcaggttca agctggtact atctccgtta catcgatcca
     1621 cacaatactg aaaaattggc tgacgaggaa ctcctcaaac aatggttgcc agttgatatc
     1681 tatgtcggtg gtgcagagca cgccgtgctt caccttctct acgctcgctt ctggcacaag
     1741 gttctttacg atctcggtgt cgtacctacc aaagagccat tccaaaaact ctttaaccaa
     1801 gggatgattt tgggaacaag ctaccgcgat agccgtggtg ctcttgtggc aactgataag
     1861 gttgaaaaac gtgatggtag cttcttccat gtggaaactg gggaagagct tgaacaagca
     1921 ccagctaaga tgtctaaatc acttaagaac gttgtcaacc cagatgatgt tgttgaacag
     1981 tacggtgctg acacccttcg tgtttacgag atgttcatgg gaccacttga cgcttcaatc
     2041 gcttggtctg aagaaggtct tgaaggctca cgtaagttct tggatcgtgt ttaccgtttg
     2101 atcacgacta aagagattac tgaagaaaat agcggagccc ttgacaaggt ttacaacgaa
     2161 accgttaagg cagttactga gcaagttgac caaatgaaat tcaacacagc tatcgctcaa
     2221 ctcatggtct ttgtcaatgc agccaacaag gaagacaaac tctttagcga ctatgccaaa
     2281 ggctttgttc aattgattgc accattcgca ccacacttgg gtgaggaact ctggcaagtc
     2341 ttgacagcct caggtgagtc aatctcttac gtaccatggc caagttatga cgaaagcaaa
     2401 ttggttgaaa acgaaattga aatcgttgtc caaatcaaag gtaaggtcaa agctaaactt
     2461 gtagttgcta aagacctttc acgcgaagaa ttacaagaag ttgcccttgc taacgaaaaa
     2521 gtccaagccg aaatcgcagg caaagacatc atcaaagtca tcgcagtacc aaacaaactg
     2581 gttaatattg tggtgaaata agataaagat tttactagaa atagtgatat ttacgcgtat
     2641 aaaatcccaa aagtttatga acttttggga tttttctatt tttagctaaa tcagtcagct
     2701 cgtactgttt ttttagcata gtaagttttt aatacctctt ttgtaatacc atgtttttga
     2761 tagatttttt ctatttttga cgttaatatt tttagttctt tattttggtt ttcaatagag
     2821 ttatcaaatt tttgatagcg gtcgttcaac cttgtctcaa ggtaagcacg ttgttcaaaa
     2881 tataatttta atctatcttt ttcgttagtg gatagggttg tcttatcaat caattttttg
     2941 acttcacttg tgctatccca tgtttgatta tcaatagaag tatagaattt agttagtaga
     3001 cgtttatttt gcttctctag ttggttgata tctttgttaa tatacaattc aataactttt
     3061 aaatcttttg cttttttgac ttcaatatgt cgttggatat ggtcgatttt agcgtagaga
     3121 ggagcagctt ctttatctgc aacttttaac ttatgttcaa tagttttttg actcatatga
     3181 acagttgatg ttttttgtga ttgttcagcc gctacatgat ttggttgtaa actcgtatat
     3241 gttgctaaag agagaacggc aataagcgat aaggaatgta tttttttaga atgtaacata
     3301 taaaaactca ctttctttta gaataacttt ttgatctata ggtattatag gttaaaaaag
     3361 caataacttc tttgaatttt tgtaggaaaa gttaggcaat tttgtggcaa ttgaaataaa
     3421 tcgtaaaagt tgttgaggtc gaatcactct cagcgatgat attaccatga tgttgcttga
     3481 taatttcttt agtcattgca agtccaatac ctgtttggcc gccatgtcct ttatagaagc
     3541 gatcgaaaat ataaggcaag tcttcttttg aaatggctgg tccgtcgttt gagatagtta
     3601 gtttttgatt tgtgtaagag atttcaatat gagaaacagc atagcgtagt gcgttactaa
     3661 ggatattatt gatagctcgt tgaatcaact cttcgttacc ttttagtaac ttttcatgct
     3721 tgtctggtct ataagtgata ctaatctttt gtttattagc taggggtttg aaataagcaa
     3781 taatactatt aagtaattca tttaaagaaa atttatcttt ttgatcactt aagttactat
     3841 ctaatttaga gagtaagata atatcgtcta cgagctgttt catttttttg ctctcttgca
     3901 gaataacaga gtgtgcgagc tcggcatcaa taataccctc ttgaactccc tcagtatatc
     3961 cttggatgga cattaaaggt gtccgtaact cgtgggaggc attttgaaag aagtttcttt
     4021 ggctctcttc tgcctggtag attcgatttg ccatttcttg gctctcaagt agaagaggat
     4081 cgaaatcaga aaagacaatt tctttttgac tagattgatg attgccttga tttttccaaa
     4141 ggctggaaat ataagtttgt actgattgaa tagattctct aattccagtg aaagtcttcc
     4201 tcataataaa tagcatggga atataggtta gtactaagat aactataaga aaatgattaa
     4261 ttttagtaat tagatgctgg gtgtaagtaa tgtctgaaaa gttaatcaca tgaaaaagtt
     4321 gcgactgtgc tttaggagct ttttttatgc taccatcaac aaggatacca gagtagtctt
     4381 tggtcattac ataataggtt tttttcttga tggttataaa cataccatct ttttccttgg
     4441 aaatttgcca caatcttttt tgggtaagat agcgaataat atcatggctc tcttttttat
     4501 gattatcagt gccatatttc atacgataat gctggtcaaa aatagcatag taagaccaaa
     4561 caaagatgct atcttggtta accatatttt catctggtat tttcttgttg ttatagatac
     4621 tatctaaaat ggtgaaattc gcctgcatgg aattccttgt catttgatgg acatagtctt
     4681 taatgacata gttgacagaa aaatagatga ggtaaaaacc aagaaataga gtgatgctat
     4741 taataaggat aagattgcta attaaacttt tctttttcat ttgtcctctt tgattaattt
     4801 gaagccgtaa ccccagacag ttttgatact aacctgactg tggtattgct taagtttttt
     4861 acgtaatcgt ttaatagtgt catcaactgc tctgctttca atatcatcaa atccccaaat
     4921 acgattaagc agtgtttccc tagaaaaagc agtttcaggt ttttcaatta atactaaaag
     4981 aaaatcaaat tctgtcttgg ttaagggcat ttcttcattt gataataggg ctattcgctt
     5041 ttcagtatcc agaattaaat ctagaactga tactgagtgt catttttgat tgctttttca
     5101 tcaaggcgtt tgaacaatgc tttaacatgt agactaagtt ttataggtga aaaaggcttg
     5161 gtgaagtaat cgtcagttcc taaattaaaa gccgtaatga aatcaacatc agaatctcta
     5221 gctgttaaca tgataatagg aatattatca tattggcgga tgcttttcat aacatctaaa
     5281 ccattagttc caggcatcat tatgtctaat ataactaaat cgggtttttg gtttttatag
     5341 gcttctaaga gaaggtctcc agtttcaaat gctcgaacag tgaaaccatc atgttctaaa
     5401 aaaggaacca ccaagtcacg aatattcttt tcatcatcag cgaaataaat tatttttgtc
     5461 atgtatacct caatacccat agttataatt tgacttttag ctagctgttt atgttatatc
     5521 aaatataaca aaaaaagagt ttttttccta ttctttgaag tgttatagtg ttataaacca
     5581 agatattgca gagaaaaggt ctttaggaac atcattttaa gctcctcaaa aaggcaccag
     5641 tcactaacta ttaatagcta gtaattgatg ccttgctcta ttttttaaag tattttattc
     5701 tcttttgata gactttagtt tgattgttgc cagacctagc atagagagac taaatagcaa
     5761 tattgtatag gttattttag gagtgctttc gcctgtttta ggaagcttgt ttgtctttat
     5821 cttttgatta tcggtttggc tagtcaccct taagacacgc ttctgagcaa cctcactttc
     5881 tttagtcatg ccttttttga atatcaacgc ctggggctga tttggcggat tgataggtgt
     5941 ggtattgaga gagtcattat cattgagtga ttgactataa ttctctgcgg aagaaactct
     6001 atcgcgacca tcaagttctt gttcggttgc ttttatttgg tttaataact cttcaacttg
     6061 agcttgtgta gcttgtctat tggaaagcag caattctgct ttcttgataa ggttattata
     6121 attttcttta gcaaattgac tggcgttctg atatttagat gatgtttgtt tcagaacatt
     6181 tgctttaatt atcatatggt gtaaaccaga gtaatcagtt tcatgaccat ttaattgatt
     6241 tttagcaata tcaagatttt ctaaggcttt gtctacttct gattgagtga cgttaggttt
     6301 ttgtagaaga tttttcgcca attggacttg attgatataa gttgattggc ttaatggact
     6361 agcattaaca aattgatagt ttcccttgat ttctgtttca gctaaaatag cttttgccag
     6421 taaagaagta tctgtttctt taccgttaag agcttggcga ctttctctca aagcagcaat
     6481 agcattatca atacttgttt gttccactct atttttaatt aaagtttgtg ccttttcaag
     6541 attagctagg taattatttt ttaagtgagt tccagcatta taaaacacag gttgggaaat
     6601 aatgtcagtt tgttctgcta gggcattaat tagaggagct ttattaatca gcgtcaattt
     6661 tgttagattt acttgacctt cttttacgga tacaagtaaa tcatctaagt tctctaaagt
     6721 gctataccca ctaggcagcg ttacgtttaa acgatagtct cctacaggta tgtttttacc
     6781 atagtcagta ggagaataag ttgcttttgg tagatcaaca acagtatttg tcttagtaac
     6841 caactggact gttgctcctt ttggtaaaag cttgtcaaat tcaacgagta aggctgcttt
     6901 cttgagtaag ttaactttaa ataagacgtc tttaaggcta tctttttcag aaatagttac
     6961 agtgacactt tggggactga ttagatttgc tcgttcctca tcgtataaga ataggtcaaa
     7021 cgtataggta ccaaaaggca atttcagtaa attgaggtct ttcccgtgat gttgcttttt
     7081 gatgacatta cctttgtcat ctttgacaga gtaagagaaa tcaatatcga cattactgtt
     7141 aagctcaggg ctaaacactt tgacgtttac taaaccattt ttattgccaa tattgatcaa
     7201 atcttcgagt tttgctgaca ctagattgcc tgcataatct tcaacagtgt agtagacatg
     7261 agagaaatca ttaacctctt taggtagaat aaagctacca tccttttctt gtttgataaa
     7321 tactttgtta tcttcaacaa ggataccgtc ttctcctttt tgacgtaaga cgctattata
     7381 atgaccatct ttatcttttt ttaagtaaaa aacttgttct ctaaagatac cagattcccc
     7441 gtgttctact gcaggacgag ctttgaagat acgtctatct ttgtcataag ttgctgttgt
     7501 tagtgtagga gcttgtctat ccaaagtgat atcaaatacc atttgttgtg gcttagagcc
     7561 agggacatca gagtagtatg ttaaaacgta tttgtatttt ccatcttcta aaggaatacc
     7621 atcagtagtg gttcctttcc attctgtgtc atataaaaat gtagattttg gattttcagt
     7681 atttccgctg tagtaatttt tatctcctgc ttggggagca cttacccata gtggtttttg
     7741 taaattaaca tcatctgcac gatagacttt ggctcttaaa ttattgaaat tacgtaagaa
     7801 aacacctttc agtgcaacgg catcttgatt ttggtcatca ttaggtgaaa tggctagatg
     7861 aggctggcct ttttgctcta gttgtaagat ccattttcca tctatagact tatagcttcc
     7921 caaggctttc aattggatag gactagtacc gtctgttgag tagataggct ctgaacttgt
     7981 agtcattaag gcagtataat cttctgaacc aggaacttcg tttgtttgtt tcggttggaa
     8041 ataaaatccc ttttctttgt tagcaacaag cttataaatg gatttttcta aaacttctaa
     8101 gttttggaat tctcccttaa atccaacata aggaatacta agaacttccc caccatcaac
     8161 agggtctgta aaacgtacgt agccttctag gaaatagccg tttggcatta ctttttttaa
     8221 catgtcatgg tatttcgaaa catctatatc aattgtaatc gttttagttt gccctggttc
     8281 aatccgtatc gttttccctg taaatgtacc aagttgttga ggagccaatg tgacaaagcc
     8341 atctttaact tgatcagtat ttaaataggt tgtatagtgt aaatcttttg caactttatt
     8401 gatattatga acagtaacat caaaggaaat tttatcttta atatttccta atgtaacgct
     8461 accatagttg ttttccccac cagttaggta taatcctgtc tgaactgctg ctgcgacatc
     8521 gataatacct gctccttgtt ggcgtggtga ggtgtaagcg cctgtatctt tgtttaggtg
     8581 tgctttagca gtactcataa gaaggtattt gacagttctt tcaatgtcac cttttttaag
     8641 ttctggatgt tctttcaata agtattgttt aactagagca gtagcaccag cgacatgggg
     8701 agaagccata cttgtcccac tcatcatatc atattcatta tcattgatag cggcgtaaat
     8761 agagcctcca ggagcagata agtcaggttt taattgtccg tctgctgtta atccccagct
     8821 tgaaaagtca gataggacac ctgcattagg gttggcttgt ttatttttga tattgttaaa
     8881 tacaatttta tagttgtttt tagctaaaac atctccaaac tctttttgag taaaaatggc
     8941 aggaatagcg cttgcttcag gatctaaact cattgttaga ttagcttctc cagctttgtt
     9001 attaaagata atagctccca cagcaccgtg attaatagcg ttgacgactt tttttgtaaa
     9061 agtaatatct ccacgttcaa ttaaggcgat tttaccattt aaagttttgt ccttataatc
     9121 attgtcgttt ccttttccta cgaaaacata atcatattgc ttccctactt caaaggtctt
     9181 atcactaact ttaggatctg cataagcagc taatccgttg ttgagatttt tgttgttttc
     9241 taatccgata atgttgaaga ctttattaac cagtgttgta ttattatatg atgctacaga
     9301 aatggcctca cgagctgttg atggactacc aactaaacca taatcaggat aaagagcaga
     9361 aggctttgat gctccactcc caaatgtacc gtcgttacct gctgctataa caacagaaac
     9421 gccagcgagt ctagccatct ctaaagcttt tataagtcgg tcatcggcat taactaaaga
     9481 gccattagct ccacctaaac ttaaattaat gctatcagca ccgagtttaa cggcatcttc
     9541 aatagcttta acataaaggg ctggtccagt tcctcttttt tcatcagaga agactctcat
     9601 aaacattact tgtgcttcag gagcaacacc atagattaat tcatttgtat ctttcttgct
     9661 aggattagct gttgcgatac tggttacgtg cataccatga gaagtgcttt tcacctcttt
     9721 tagctctgta ttgacatcaa catagttgtg accaaagatt actttgttgt tataccattt
     9781 tccatagttt ataccagctt ttgctttagc agcattcatt tgctgttcgt tttggtattt
     9841 tgcttttgtc gaatcattta attggagagc atcgtgattg gtatctagtc ctgagtcaat
     9901 aattgctaca acagttccct ctcctttgta gcctgtgttc catactttgg ggacagtgat
     9961 aattttatta ctatcaatgt tttgaggggt ttctttagaa atagctggtg taggtttgac
    10021 tgctaaattt tgtttggtta aaggagtttt agaaacttct tgagtgcttt caaccggttg
    10081 atttgcggct ggggaaaatg tctcggtggc ttgagagtga ggctcagtta ctggttgtgt
    10141 ttctgttgga ctattattat tagcctttgt aacaagattg tcttgagctg ttgacgcaag
    10201 ggctgatgac gtgttgttag taggtgtctc atttgctatg ctcgttggac tgtcttttac
    10261 ttgctgttca actgcaatat cagatggctt atttatgaca gaatcagcag ctacatttgt
    10321 gataccgcct aaaaaaaata gggttcccaa aagtacagat acggcaccta acttatattt
    10381 ccggattgaa aaacgctgtt ttgtgttcat aaaaatctcc ttttcttgta acataataat
    10441 tacaaaaact attaaaacat aaaaaatcaa aattttctac aattttctga attttcatta
    10501 caaattattt tgtggtcaat ttaagaccgt acctaaagca ttttttaaaa atcattctat
    10561 gaaaataata atattgtcca actatatggc tacaatatca aaggtttata gaataattac
    10621 aggatattta ttagtctaat tgctggcaaa gctaaataat cattcctctt ctttcttagt
    10681 aatcaccgtt gtgctataat aactgaatat actgaaaatt tagggggata tgatggaaaa
    10741 aggatttaaa gagggagtga aagatgcctt gcccacagct ttagggtata tctcaatagg
    10801 cttggcgttt gggatagttg cttcagcatc agatttatct gcaatagaag ttggtcttat
    10861 gagtgccttg gtatatgggg gatctgctca gtttgctatg tgtgcgctat tattagcaaa
    10921 agcagacttg atgactatta cgatgacagt atttttagtt aatttgagaa atatgttaat
    10981 gagtttacac gcgactacta tcttcaagtc tgctcaccta atgaatcaat tagccatagg
    11041 aactctaata acagatgaga gctacggcgt tttacttgga gaagcattgc atcacaaagt
    11101 cgtatcgcct agttggatgc atggtaataa tgtgatgagt tatttgacat gggtaatctc
    11161 aacgataata ggaactttac tggggagtac tattcctaat ccagaaatgt ttggtttaga
    11221 ctttgcatta gtcgccatgt ttataggtct ctttgtattt cagttgtttg ggatgttatc
    11281 tgatggaaag cgacttgttg tatatgttct tgcttctgta ggtttaagtt acttcttatt
    11341 atccactttt ttgtcaggag ccttatcagt attgcttgcg actgttgttg gatgtagtgt
    11401 aggagttgtt ttagatgata aatagtaccg tttttcttgc tattctttta ggttttgttg
    11461 tttcttgggt gccaagagtc gctccattca tatttgtaaa atacaaaggg ctaccaaata
    11521 gtcttgtgaa atttttaaaa tttcttcctc tttctatttt atttgcccta acattctcta
    11581 gctttttttc agttaaatta ggacaattgc caacactaaa atggttagat ttattggctg
    11641 gcataccaac tctagttgtc actattcgat atcgaaattt attttacact gttcttactg
    11701 gtatcgttac tatggcttta ttgagattac tattttaacg tgtaggaatg gaagattaat
    11761 aaaaacacaa aaaacgcatc tatttctaga tacgtttttt gtgtttttgt ctagacagga
    11821 acttttgata gccgatgtgt aatagtttga ctaattgtta ataatatcaa cagatttttc
    11881 tgtatcttga taatatgagt tgattgtaag attgcttttt aaagatatct acatgctgct
    11941 attttaaaat ttataaaaag g
//
LOCUS       DATROD010000048        11726 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.048, whole genome shotgun sequence.
ACCESSION   DATROD010000048 DATROD010000000
VERSION     DATROD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11726
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.048"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            58..543
                     /locus_tag="VBC74_001212"
     CDS             58..543
                     /locus_tag="VBC74_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942162.1"
                     /translation="MRETETHDHQALIQKLLVSIHYLTLFRDEIILVEKTPSLLGKHF
                     SIAIVQNELGEILSKIEALSKQKKLIRSIYWYDESSFKVMNKALAIVEEWIKGLDNLL
                     EFCQSQTVFQAILGDERAHVFGILIDVYTSLNIINTSLKEENSRPVSLSDLALGLKDE
                     D"
     gene            642..1295
                     /locus_tag="VBC74_001213"
     CDS             642..1295
                     /locus_tag="VBC74_001213"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEO6942163.1"
                     /translation="MKHSSWHDLIKRELPNHYYNKINTFMDAVYESGIVYPPRDKVFN
                     AIQITPLENVKVVIIGQDPYHGPQQAQGLSFSVPDNLPAPPSLQNILKELAEDIGSRS
                     HHDLTSWAQQGVLLLNACLTVPEHQANGHAGLIWEPFTDAVIKVVNQKETPVVFILWG
                     GYARKKKSLIDNPIHHIIESPHPSPLSAYRGFFGSRPFSRTNHFLEEEGINEIDWLN"
     gene            complement(1361..1999)
                     /gene="plsY"
                     /locus_tag="VBC74_001214"
     CDS             complement(1361..1999)
                     /gene="plsY"
                     /locus_tag="VBC74_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEO6942164.1"
                     /translation="MNIIIMIIIAYLLGSIQTGLWIGKYFYQVNLRQHGSGNTGTTNT
                     FRILGVKAGIVTLTIDILKGTLATLIPIILGVTTVSPFFIGFFAIIGHTFPIFAQFKG
                     GKAVATSAGVLLGFAPSFFLYLLVIFLLTLYLFSMISLSSITVAVVGILSVLIFPLVG
                     FILTDYDWIFTTVVILMALTIIIRHQDNIKRIRKRQENLVPFGLNLSKQKNK"
     gene            2113..4062
                     /gene="parE"
                     /locus_tag="VBC74_001215"
     CDS             2113..4062
                     /gene="parE"
                     /locus_tag="VBC74_001215"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEO6942165.1"
                     /translation="MTKQDITVTNYGDDAIQVLEGLDAVRKRPGMYIGSTDGTGLHHL
                     VWEIVDNAVDEALSGFGNRIDVIINKDGSITVTDHGRGMPTGMHAMGKPTVEVIFTVL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEIIRDGAIYRQRFENGGKPVTTLK
                     KIGTAPKSKSGTSVSFMPDQSVFSTIDFKFNTIAERLKESAFLLKNVTLTLTDNRSEE
                     AEHLEFHYENGVQDFVEYLNEDKETLTPIMFFEGEEQEFHIEVALQYNDGFSDNILSF
                     VNNVRTKDGGTHETGLKSAITKSMNDYARKTGLLKEKDKNLEGSDYREGLSAILSILV
                     PEEHLQFEGQTKDKLGSPLARPIVDGIVSEKLTYFLMENGDLASNLIRKAIKARDARE
                     AARKARDESRNGKKSKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTAKAKMADIIKNEEINTMIHTIGAGVGPDFNLDDINYDKVIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVEEGHVYIALPPLYKMSKGKGKKEIVEYAWTDIEL
                     EELRQKFGKGSLLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLM
                     GDKVPPRRQWIEDNVKFTLEENTVF"
     gene            4196..6655
                     /gene="parC"
                     /locus_tag="VBC74_001216"
     CDS             4196..6655
                     /gene="parC"
                     /locus_tag="VBC74_001216"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEO6942166.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGFRKSAKSVGNVMGNFHPHGDSSIYDAMVRMSQDWKNRETLIEMH
                     GNNGSMDGDPAAAMRYTEARLSEIADYLLQDIDKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGATGISAGYATDIPPHNLAEVIDAVVYMIDHPKAKLDKLMEFLPGPDFPTGAI
                     IQGKDEIRKAYETGKGRVAVRSRTAIETLKGGKKQIIVTEIPYEVNKSVLVKRIDDVR
                     VNNKVPGIAEVRDESDRDGLRIAIELKKEADETIVLNYLFKYTDLQVNYNFNMVAIDD
                     YTPKQVGLSRILTSYIAHRREIIIARSKFDKEKAEKRLHIVEGLIRVLSILDEVIALI
                     RASENKADAKENLKVSYEFSEAQAEAIVTLQLYRLTNTDIVTLREEEEELRQQITMLK
                     AIISDERTMYNVMKRELREVKKKFANTRRSELQELAETIEIDTASLIIEEDTYVSVTR
                     GGYVKRTSPRSFNASTVDELGKREDDELIFVSNAKTTQHLLMFTNLGNLAYRPVHELA
                     DIRWKDVGEHLSQNLVNFASNEEIIYAELVDDFTKETYFAVTSLGQIKRFERQEISPW
                     RTYKSKTAKYAKLKNVDDYVVTVAPIQLEDVILVTYNGYVLRFSINDVPVVGSKAAGV
                     KAMNLKDSDHIVSAFIANTTSLYLLTHRGSLKRMAIDVIPTTSRANRGLQVLRELKSK
                     PHRVFKAGPVYLEDSSFEFDLFSSVSNHEGDTFVLEIMSKTGKVYDVDLSQWSFSERT
                     SNGSFVSDKISDEEVFSVKIK"
     gene            6769..7791
                     /locus_tag="VBC74_001217"
     CDS             6769..7791
                     /locus_tag="VBC74_001217"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEO6942167.1"
                     /translation="MTVNLDWDNLGFAYRKLPFRYISHFKDGKWDDGKLTDDATLHIS
                     ESSPALHYGQQAFEGLKAYRTKDGSIQLFRPDQNAERLQRTADRLLMPHVPTDKFIAA
                     VKSVVRANEEFVPPYGTGATLYIRPLLIGVGDIIGVKPAEEYIFTVFAMPVGSYFKGG
                     LTPTNFIVSKEYDRAAPNGTGAAKVGGNYAASLLPGKYAHEKQFSDVIYLDPATHTKI
                     EEVGAANFFGITKDNQFITPLSPSILPSITKYSLLYLAKERFGMEAIEGDVFVDELDK
                     FTEAGACGTAAVISPIGGIQNGDDFHVFYSETEVGPATRKLYDELVGIQFGDVEAPEG
                     WIYKVD"
     gene            7874..8104
                     /locus_tag="VBC74_001218"
     CDS             7874..8104
                     /locus_tag="VBC74_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001870505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEO6942168.1"
                     /translation="MSKKDKKIEIQILDAQVTVNGTKVDGYQLVIGKKIVGQIAELDS
                     KFAVIKGEQVSGFYKNLDQAMGNIIEEYNLNR"
     gene            8165..8245
                     /locus_tag="VBC74_001219"
     tRNA            8165..8245
                     /locus_tag="VBC74_001219"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8199..8201,aa:Tyr,seq:gta)
     gene            8251..8322
                     /locus_tag="VBC74_001220"
     tRNA            8251..8322
                     /locus_tag="VBC74_001220"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8283..8285,aa:Gln,seq:ttg)
     gene            8448..9650
                     /gene="rpsA"
                     /locus_tag="VBC74_001221"
     CDS             8448..9650
                     /gene="rpsA"
                     /locus_tag="VBC74_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEO6942169.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIDGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVVEESAAAARKEVFSNIEVGSVVTGKVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVTVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AAGDVIEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSAGQEVTVKVL
                     EVNSDAERVSLSMKALEERPAQAEGEKEEKRQSRPRRPRRQEKRDYELPETQTGFSMA
                     DLFGDIEL"
     gene            9723..9794
                     /locus_tag="VBC74_001222"
     tRNA            9723..9794
                     /locus_tag="VBC74_001222"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9756..9758,aa:Arg,seq:ccg)
     gene            complement(10063..10686)
                     /locus_tag="VBC74_001223"
     CDS             complement(10063..10686)
                     /locus_tag="VBC74_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEO6942170.1"
                     /translation="MIPLKKITVGTIVCLSFLGLTACSSSNTQQTSTSKSNVSQHKNI
                     KADHEELRLKFNKVKLGVKANNFKGGTSLAELKQLFGGEPNEKFDTPAGNVTLKGYRW
                     NVDDISITIQLLNDSSIVRSISNFKFIRDANITTKDYNSLKNGMSYNKVKELLGEPDD
                     ISQAVSSDKEELQAAWISGIQSSDSDPGINLTFENDKLTNKQQHGLK"
     gene            complement(10755..11450)
                     /locus_tag="VBC74_001224"
     CDS             complement(10755..11450)
                     /locus_tag="VBC74_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEO6942171.1"
                     /translation="MATLTNNPIFGIMLTVWAYYIGIRIFRKYPSPATTPLLLATILL
                     IAFLKLTHISYKDYYNGGSFLTMLITPSTVVLAIPLYRTFHLMKHHIKSISISIILAS
                     VINTVFTAIVAKFFGMKYFLAISLFPKSVTTAMAVGITSKAGGLATITLVVVVITGIL
                     TSVLGPIFLKLLKIEDPVAIGLALGGTGHAIGTGQALKYGQVQGAMAGLAIGITGICY
                     VIVSPLVAGLILK"
     gene            complement(11431..>11726)
                     /locus_tag="VBC74_001225"
     CDS             complement(11431..>11726)
                     /locus_tag="VBC74_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEO6942172.1"
                     /translation="GLILLFLALKYKIIRLRHIDTVGNFLLANMTILFLPPAVGLMEH
                     FQDIKPYLFGIAIIILGALFLNILTIGLVSQWIKKRYEGDYPEIGGKNGNFNE"
BASE COUNT         3881 a         1862 c         2336 g         3647 t
ORIGIN      
        1 tatttaaagt aagctacgta tagttttacc agtcagaaat agtaaggatg gttgagtatg
       61 agagaaactg aaacacacga tcaccaagcg ctaattcaaa aattgttagt cagtattcat
      121 tatttaacct tgtttcgtga tgaaattata ttagtagaaa aaaccccatc tttactgggg
      181 aaacattttt ctattgctat tgtgcagaat gaactaggtg agatactttc taaaatagaa
      241 gctttgagta agcaaaaaaa actgattcgt tctatttatt ggtatgatga atcgtcattt
      301 aaagtcatga ataaggctct ggctattgta gaagaatgga ttaagggctt agataatctt
      361 ttagaattct gtcaatcaca gacggttttt caagcaattt taggtgatga acgtgcacat
      421 gtatttggaa tccttattga tgtttatact tctttaaaca ttataaatac ttctttgaaa
      481 gaagaaaatt ctagaccagt gtccttatct gatttggctc taggtcttaa ggacgaagat
      541 taataaaaaa acgttactgc tttgaatgaa taaagcagta acgtttttta taaaatattg
      601 ctatactagt aatacagata tctaaaagta aggagttata aatgaaacat tccagttggc
      661 atgatttaat aaaaagagaa ctacccaatc attactacaa taaaattaat acatttatgg
      721 atgcagttta cgaaagcgga atcgtttatc ctccaagaga taaggtgttc aatgccatac
      781 agataacacc actagaaaac gtaaaagtag tgattattgg ccaagatcct taccatggcc
      841 ctcaacaagc tcaaggactc tcattttctg ttccagacaa tttaccagct cctccttcac
      901 ttcaaaatat tctaaaagaa ttagcagaag atattgggtc acgttcacat catgacttaa
      961 catcatgggc gcaacaagga gtgctgctat tgaatgcttg tttaacagtt ccagaacacc
     1021 aagctaatgg tcatgctggg cttatatggg aaccttttac tgatgctgtc atcaaagtgg
     1081 ttaaccaaaa ggaaacccca gtagtcttta ttctgtgggg tggctatgct cgaaagaaaa
     1141 agtcgctaat tgataatcct attcatcata ttattgagag tcctcatccg agtcccctat
     1201 ctgcttatcg tggtttcttt ggtagcagac ctttttctag aacaaatcat ttcttagaag
     1261 aagaaggaat aaatgagatt gattggttaa attagaaggg aaatctactc tcgaaacatt
     1321 gttagtctta taataaaaaa ccgctagtag cggtttttta ttatttattt ttttgtttac
     1381 ttaggtttaa gccaaacgga accaaatttt cttgcctttt tcgaatacgt ttgatattat
     1441 cctgatgtcg gataataatc gtgagggcca ttaagataac cacagtggta aatatccagt
     1501 catagtccgt taatataaaa cctactaatg gaaaaattaa gacactaagg atacctacga
     1561 cagctactgt aatactggat agcgaaatca tgctaaaaag ataaagcgtt aataaaaaga
     1621 tcactaataa gtaaaggaaa aaagaaggtg caaatcctag caaaacccca gcgcttgtag
     1681 caacggcttt cccacctttg aattgtgcaa aaatagggaa agtgtgacct ataatagcaa
     1741 aaaaaccgat aaaaaatgga gatactgtcg ttacacctaa tatgatagga ataagtgttg
     1801 ctagagtccc ttttaaaatg tcaatagtca aagtgacaat gccagctttt acacctaaaa
     1861 tacggaaggt gttcgtggtg ccagtattac cactaccgtg ttgacgaaga ttgacttggt
     1921 aaaaatattt accaatccaa agcccagttt ggatagagcc taacagatag gcaatgataa
     1981 tcataattat aatattcata cccttattat aacataagca taaaagtctt tttgctattt
     2041 ttaaaaaaat aattttgcaa aaacagcaaa aaacttgtaa tataaaaaag atgaaaaaat
     2101 ttggaggtca ctttgactaa acaagatata acagtgacga attatggcga cgatgctatt
     2161 caagtcttag aaggattaga tgctgttcgt aagcgtccag ggatgtatat cggttccaca
     2221 gatggaactg gtttacatca tttagtgtgg gaaattgtag ataatgcagt tgatgaagct
     2281 ctatcaggtt ttggaaatcg cattgatgtg attattaaca aagatgggag tataactgtt
     2341 acagaccatg gacgtggtat gccaactgga atgcatgcta tgggaaaacc tactgttgaa
     2401 gtcatcttca cggtgcttca tgcaggtgga aaatttggac aaggtggtta taaaacatca
     2461 ggtggtctcc atggtgttgg atcatctgta gttaatgctt tgtcttcttg gttagaagtt
     2521 gaaatcattc gcgacggagc tatttacaga caacgttttg aaaatggtgg taagcccgtt
     2581 acaactctga aaaaaattgg cacggcacca aaatcaaaat ctggaacttc tgtcagcttt
     2641 atgccagatc aatcagtatt ttcaacgata gattttaagt ttaatacaat tgcagagcgc
     2701 ttaaaggaat ctgcctttct cttaaaaaat gttactttaa ccttaacaga taatcgttca
     2761 gaagaagcag agcatctaga atttcattat gaaaacggtg ttcaagattt tgtagagtat
     2821 ttaaatgaag ataaagaaac gttgacacca atcatgtttt tcgaaggtga agaacaagaa
     2881 tttcatatcg aagttgcttt gcagtataat gatggttttt cagataatat cctttctttt
     2941 gtcaataatg ttcgtactaa agatggtgga acacatgaaa ctggcttgaa atcagctatt
     3001 acaaagtcta tgaatgacta tgcacgtaaa acaggacttc ttaaagaaaa agataaaaac
     3061 cttgaaggtt ctgattatcg tgaggggcta tcagccattt tatctattct tgtacctgag
     3121 gagcacttgc aatttgaagg tcaaactaaa gataaactag gaagtccctt agcacgacca
     3181 attgttgatg gtattgtttc tgaaaagtta acttactttc ttatggaaaa tggagattta
     3241 gcatctaatt taatccgtaa ggcaataaaa gcacgtgatg cgcgtgaagc tgcacgtaaa
     3301 gcacgtgatg aaagtaggaa tgggaaaaag tcgaaaaaag ataaaggtct actttctgga
     3361 aaattaaccc ctgcacaatc aaaaaatgct aagaaaaatg aactctattt agtcgaagga
     3421 gactctgcag gtggctctgc taagcaaggt cgtgatagaa agtttcaagc tattttaccg
     3481 ttgcgaggaa aagtattgaa tacagctaag gcaaagatgg ctgatattat aaaaaacgaa
     3541 gaaattaata caatgatcca taccattggt gcaggtgttg gtcctgattt taatcttgac
     3601 gatattaatt atgataaagt tatcattatg accgatgctg atactgacgg agctcatatt
     3661 cagacactat tattaacttt cttttatcgc tatatgagac ctcttgttga agagggacat
     3721 gtctatattg ctttgccacc tttgtataaa atgtcaaaag gaaaaggtaa gaaagaaatt
     3781 gttgagtatg cttggacgga tatagagtta gaagaattac gacaaaaatt tggtaaaggc
     3841 tcactattac aacgatataa agggcttgga gaaatgaatg ccgatcaatt gtgggaaacc
     3901 actatgaacc ctgagacaag gacattaatt cgtgtaacaa ttgaagattt agcaagagca
     3961 gaacgtcgag ttaatgtctt gatgggtgat aaagttcctc caagacgcca atggattgaa
     4021 gataatgtca agtttacttt agaagagaat acagtatttt aataaagagc cttggttatt
     4081 catgttttat agtgtacaag taatattgta tgcgttagac aagagagcca atcattcatt
     4141 tcaaataagt ggaaaaataa tactaagcca ctccaactag gagaaaatta aaaatatgtc
     4201 taatattcaa aatatgtccc ttgaggacat tatgggagag cgttttgggc gctattctaa
     4261 gtatatcatt caagaacggg cgttgccgga tattcgtgat ggcttaaaac ctgttcaacg
     4321 tcgaatcctt tattcaatga ataaagatgg taatactttt gaaaagggat ttcgcaaatc
     4381 tgcaaaatca gtcggtaatg ttatggggaa tttccatcct catggggatt cctctatcta
     4441 tgacgcgatg gttcgtatgt ctcaagattg gaaaaatcgt gagacattga ttgaaatgca
     4501 cggaaataat ggttctatgg atggagatcc tgcggcagct atgcgttata cggaagcccg
     4561 tctatcagaa attgctgatt atcttcttca agatattgat aagaatactg taccctttgc
     4621 gtggaatttt gatgatacag agaaagaacc aactgtttta ccagctgcct ttcctaatct
     4681 tttagtcaac ggagcaacag gaatatctgc aggctatgcg acagatattc caccacataa
     4741 tttagcagaa gtcattgatg ctgtcgtgta catgattgat caccctaaag ctaaattaga
     4801 taaattaatg gaatttctac ctggtccaga ttttccaact ggcgctatca ttcaaggaaa
     4861 agatgaaatt cgtaaggcat atgagactgg taaggggaga gtagcggttc gctcgcgaac
     4921 tgctattgaa accttaaaag gtggtaagaa acaaattatt gttactgaaa ttccttatga
     4981 agttaataag tcagttttag taaaacgaat cgatgatgtt cgtgttaata ataaagttcc
     5041 aggaatagca gaagtgcgtg atgagtctga tagagatggc cttcgtatcg ctattgagct
     5101 caaaaaagaa gccgatgaaa caattgtctt aaattatctt tttaaatata cagacctaca
     5161 agttaattat aatttcaata tggttgctat tgatgactat acccctaagc aggtgggtct
     5221 atcaagaata ttaactagct atatcgctca ccgaagagaa attattattg ctcgttcaaa
     5281 atttgataaa gaaaaagccg aaaaacgtct ccatattgtt gagggactga ttagggttct
     5341 ttctattttg gatgaagtga ttgcattaat tcgtgcttct gagaataaag ctgatgcgaa
     5401 agagaatttg aaggtcagct atgagttttc agaagctcaa gcagaggcta tcgtcacatt
     5461 gcaattatat cgtctcacaa atactgatat tgtgacgttg agagaagaag aagaagaact
     5521 tcgtcaacaa atcacaatgc tcaaggcaat tattagtgat gaacgtacga tgtataacgt
     5581 aatgaaacgt gagttacgtg aggtcaaaaa gaaatttgcc aatactagac gttctgaact
     5641 tcaagaatta gctgaaacta ttgaaatcga tactgcaagt ttaattatcg aagaggacac
     5701 atacgtcagt gttactagag gcggatatgt aaaacgcacc agccctcgct ctttcaatgc
     5761 atcaaccgtt gatgaacttg gtaagcgaga agatgacgaa ttgatatttg tgagcaatgc
     5821 aaagactacc caacatttat tgatgtttac taaccttggt aatcttgctt atcgtccagt
     5881 tcatgaatta gctgatattc gatggaaaga cgttggtgaa catttatcac aaaatcttgt
     5941 taactttgct tcaaatgaag aaattattta tgctgaatta gtagatgatt ttactaaaga
     6001 aacttatttt gctgtcacta gtttagggca aataaagcgt tttgaacgtc aagaaatcag
     6061 tccatggcgg acatataagt caaaaacagc taaatatgct aagttaaaaa atgtagatga
     6121 ttacgttgtg actgttgctc ctatccaatt agaggatgtt atacttgtca cttataatgg
     6181 ttatgtgttg agatttagta taaatgatgt accagtcgtt ggtagtaaag ccgcaggtgt
     6241 aaaagcgatg aatttaaaag atagtgatca catcgtatct gcatttatag ctaacaccac
     6301 aagtttatat ttattaactc atagaggaag tcttaaacga atggccattg atgttatccc
     6361 cacaactagt cgtgctaatc gaggacttca agttcttcgt gaattgaaat ccaaaccgca
     6421 tcgcgttttt aaagcaggac ctgtatattt agaggattca tcttttgaat ttgatttatt
     6481 tagttcagta tcgaatcatg aaggagatac tttcgtactt gagattatgt ctaaaacagg
     6541 aaaagtttac gatgttgatt taagtcaatg gagtttttct gaacgtacaa gtaatggctc
     6601 ttttgtatct gataagattt ctgacgaaga agtattctca gttaaaatta agtaaaaaca
     6661 tatttgtgtt tttactagtt tggtaattta tctgaatttt acaaataata ttgaaaaaat
     6721 aatgataata gcgtacaata gttagaaacc caaagaaaga ggtaccttat gacagtaaat
     6781 ttagattggg ataatttagg ttttgcttac agaaaattac cattccgata catttcgcat
     6841 tttaaagatg ggaaatggga tgatggcaag ttgacagatg atgcaacgct acatatttca
     6901 gaaagttctc ctgccctcca ctatgggcaa caagcttttg aaggattgaa agcttataga
     6961 actaaagatg gctctattca gttatttcgt ccagatcaaa atgcagaacg acttcaaaga
     7021 acagcagatc gtcttttaat gccacatgtt ccgacagaca aatttattgc agcggttaaa
     7081 tctgttgtac gcgctaatga ggagtttgtt ccaccgtatg gtacaggggc aacattatac
     7141 attcgtccct tattaattgg tgtgggagat attattggag ttaaacctgc tgaagaatat
     7201 atttttacgg tatttgcaat gccagtaggt agttatttta aaggtggttt aacaccaact
     7261 aactttattg tttccaaaga atatgataga gcagcaccga atggtacagg agctgcaaaa
     7321 gttggtggta actatgctgc tagtcttctt cctgggaaat acgctcatga gaaacaattt
     7381 tcagatgtta tctatcttga tccagcgaca catactaaaa ttgaagaggt tggagcagct
     7441 aatttctttg gaattactaa agataatcag tttatcaccc ctttgagtcc ttcaatttta
     7501 ccttcaatta ctaaatattc attgctttac cttgctaaag aaaggtttgg tatggaagcg
     7561 attgaaggag atgtctttgt cgacgaatta gataaattca cagaagctgg cgcatgtggt
     7621 acagccgcag ttatttcacc aattggagga attcaaaacg gagatgactt ccatgtcttc
     7681 tacagtgaga cagaagttgg tccagcgaca cgtaaactat atgatgagtt agtagggatc
     7741 cagtttggtg atgttgaagc accagaaggg tggatttata aagtagatta agtctatact
     7801 aaaagcttgc tcttgcaagc ttttagtatt ttgtgtacaa taaaaaacaa taaaagagat
     7861 attggagtta atagtgagta aaaaagataa gaaaattgaa attcaaattt tagacgctca
     7921 agtaacagtt aatggaacta aggttgatgg ataccaactc gttattggaa aaaaaattgt
     7981 aggtcaaatt gcagagctag attctaaatt tgctgtgatt aaaggtgagc aagtgagtgg
     8041 attctataaa aatttagatc aagctatggg aaatattatt gaagaatata atcttaatcg
     8101 ctaatattta tttaaaaagc ttgcgtgcta acaacatctg tggtagaata gtcagtgttg
     8161 ttacggaaag atagcgaaga ggctaaacgt ggcggactgt aaatccgctc cttcgggttc
     8221 gggggttcga atccctctct ttccattatt tggggtatag ccaagcggta aggcaaggga
     8281 ctttgactcc ctcatgcgtt ggttcgaatc cagctacccc agtcaaaggt atcaaacttt
     8341 tgtttgacac cgtcaaagag atatttgttg tccttgcggg ttaccttgag aaaatatatt
     8401 gttatcaaaa gctagctcgg ctagcttttg ttggaggatt ttttagaatg aatgaatttg
     8461 aagatttgct aaacagtgtt agcgaagtga atcctggtga cgttgtcact gcggaagttt
     8521 taacagttga taacggtcaa gcaaacgttg ttatcgatgg tactggtgtt gaaggtgtct
     8581 tgacacttcg tgaattgact aacgatcgtg atgctgatat caatgatttt gttaaagctg
     8641 gcgacacagt tgaagtactt gttcttcgtc aagttgtagg taaagatact gatacagtaa
     8701 ctttccttgt atctaaaaaa cgcttggaag ctcgtaaagc atgggacaaa cttgttggtc
     8761 gtgaaggtga agttgttact gttaaaggta cacgtgctgt taaaggcgga ctttcagttg
     8821 aatttgaagg acttcgtggt ttcatcccag cttcaatgat tgatactcgt tttgtacgta
     8881 acactgaaaa atttgttgga caagaatttg atgctaaaat caaagaagtt gatgcagcag
     8941 aaaatcgctt catcctttca cgtcgtgaag ttgttgaaga atcagcagct gctgcacgta
     9001 aagaagtttt ctcaaacatt gaagtaggtt cagttgtaac tggtaaagtt gctcgtttaa
     9061 caagctttgg tgctttcatt gaccttggtg gtgttgatgg acttgtacac gtaactgaat
     9121 tatcacatga gcgcaatgtt tcacctaaat cagttgtaac tgttggtgaa gaagttgaag
     9181 ttaaagtact ttcaattgat gaagaagctg gtcgtgtatc actttcactt aaagcaacta
     9241 cacctggccc atgggatggc gttgaacaaa aacttgctgc tggtgatgtt attgaaggta
     9301 aagtaaaacg cttgactgac tttggtgctt ttgttgaagt gttacctggc attgatggac
     9361 ttgtacacat ttcacaaatt tcacacaaac gtgttgaaaa tcctaaagac gtattatctg
     9421 caggacaaga agttactgtt aaagttcttg aagttaattc agatgcagaa cgtgtatcac
     9481 tttcaatgaa agctcttgaa gagcgtccag cgcaagcaga aggtgaaaaa gaagaaaaac
     9541 gtcaatcacg tccacgtcgt ccacgtcgtc aagaaaaacg cgactatgag cttccagaaa
     9601 cacaaactgg attctctatg gctgacttat tcggtgatat tgaattataa taattaaaag
     9661 acttgctaaa gcaagtcttt ttgtttttgt tgataaagta tgctataatg taacatgtac
     9721 taccatcttt agtgtaatgg atatcacaca agattccggt tcttgggata ggggttcgat
     9781 tcccttaaga tggataatag atgctccata aagctttatg ttataggctt tatattagtt
     9841 tatctccaac cttgcctcac agactgattc tcctcatttc tgataagtaa aactagggtt
     9901 gacaaataag ggagaatcag tttgaaatct gtgtaagtta gtatcataat tagttagtat
     9961 gaccattaag gggctaagtt aaggtactaa gattaaacca ttgtgtaata aaatgtttct
    10021 aataagtaat taaggtatta ataaaaaggc gttaatgcct ttttatttta aaccatgctg
    10081 ttgtttgtta gtgagtttat cattttcaaa tgtaaggtta attcccgggt cactgtctga
    10141 cgactggatg ccactaatcc atgcagcctg tagttcctct ttatctgaag aaactgcttg
    10201 agaaatatca tcaggttctc ctagaagttc ttttacttta ttgtatgaca tgccattttt
    10261 taaactatta taatcttttg ttgtgatgtt ggcatcacga ataaacttaa aattactaat
    10321 tgaacgaaca atgctactat cgtttaataa ttgtatagtg atggagatat catcgacatt
    10381 ccaacgatag cctttaagag tgacatttcc cgctggtgta tcaaattttt catttggttc
    10441 gccaccaaat aattgtttta attcagcgag actagtgcct cctttaaagt tatttgcttt
    10501 aacccctaac tttactttat taaatttcaa acgtaattct tcgtggtcag ctttgatatt
    10561 tttatgttgt gatacatttg attttgaagt agaggtttgc tgagtatttg aagaagagca
    10621 tgctgttaaa cccaaaaatg agagacagac aatcgttcca acagttattt tttttaacgg
    10681 tatcataatt atttatccta aattctattt ttaaatatta taacataatt attttgggtt
    10741 tacttttctt ttcctcattt caatattaag cctgcaacta aaggactgac aataacatag
    10801 caaattcctg tgataccaat tgctagtcca gccatggcgc cttgaacttg gccatatttt
    10861 aaggcttgtc cagttcctat agcatgccca gttcctccta gcgcgagccc tatagcaaca
    10921 ggatcttcta tttttaataa ttttagaaaa ataggcccca aaacgcttgt taagatgcct
    10981 gtaataacaa ctacgaccag agtgatagta gctaatccac ctgctttact tgtaattcca
    11041 acggccatag ctgtagttac ggactttggg aaaagtgaga ttgctaagaa atatttcata
    11101 ccaaaaaact tagcaactat tgcggtaaaa actgtgttga taacagaagc taagatgatg
    11161 ctaatgctaa tacttttaat gtgatgcttc attaaatgaa aggttctata gagaggaata
    11221 gccaatacaa ccgttgaagg cgtaattaac atcgttagaa atgaaccacc attgtagtaa
    11281 tctttatagg agatatgtgt taattttaaa aaagcaatta ataaaatagt cgccaaaaga
    11341 agtggagttg ttgcagggga aggatatttt ctaaaaatcc ttatgccaat gtaatacgcc
    11401 catacggtta acataatacc aaagataggg ttattcgtta aagttgccat ttttacctcc
    11461 aatttctgga taatctcctt catatctctt tttgatccat tgggatacca gtccaatagt
    11521 tagaatattg agaaatagcg ctcctaagat aatgatagct atcccaaaaa gatagggctt
    11581 tatgtcttga aagtgctcca tcagtccaac tgctggtggt aaaaataaaa tggtcatatt
    11641 tgctaataga aaattcccca cagtatcaat atgcctgagc ctgatgattt tgtattttaa
    11701 cgctaggaat agtaatatta atccaa
//
LOCUS       DATROD010000049        11703 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.049, whole genome shotgun sequence.
ACCESSION   DATROD010000049 DATROD010000000
VERSION     DATROD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11703
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.049"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..611
                     /locus_tag="VBC74_001226"
     CDS             <1..611
                     /locus_tag="VBC74_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEO6942173.1"
                     /translation="TQKVIVQKKNTYHKIKSMLPKEQQEKIGYLGFIYPNKKNNKGRK
                     DIFILTNSDQIEHLEVLVHHLSDYHFHIAAYTEMSFKLMSFSQEQNVTLYPNISRTDL
                     DNLFEICDIYFDINHGNEVDDVIRRAFEYNHLIFAFDNTCHNRELVLDSNIISHTTCE
                     QLINLMKNLSGSIMYLLEQQREQTSNETKERYKEILGGYGNA"
     gene            604..771
                     /locus_tag="VBC74_001227"
     CDS             604..771
                     /locus_tag="VBC74_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942174.1"
                     /translation="MPKRNELLNKEIKMSIDKLRYKEPESEHDKRPTFYLVVLILVTV
                     AVILSLFKYFL"
     gene            825..1820
                     /locus_tag="VBC74_001228"
     CDS             825..1820
                     /locus_tag="VBC74_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO6942175.1"
                     /translation="MRTYITNLNGHSITSTAQIAQNMVTDIAVSLGFRELGIHSYPID
                     TDSPEEMSKRLDGICSGLRKNDIVIFQTPTWNTTTFDEKLFHKLKIFGVKIVIFIHDV
                     VPLMFDGNFYLMDRTIAYYNEADVLIAPSQAMVDKLQSYGLTVKKILVQGMWDHPTNI
                     TLQAVNHKKLVHFPGNPERFNFIKNWRIPTELHVYTDHNMQLPTTVVKEPYQSDEQLI
                     MKMSEGGYGLVWMDDRDKQYQSLYCPYKLGAYIAAGIPVIIQKGIANQDIIEKNNLGF
                     IIEKIDDISNIVESTTEEEYMEIVSDVRRFNPLVRQGYFTRKLLTDAVFSALNSM"
     gene            1828..3540
                     /locus_tag="VBC74_001229"
     CDS             1828..3540
                     /locus_tag="VBC74_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEO6942176.1"
                     /translation="MKELEEIVVFPINKSLDYILENHSSVIRFGDGEFDIIMGKSIPY
                     QDYNPELADRLLQLLHKQSTNDLLVCLPDMFQNRQRYNQSAQLFWKNHFQKFGNFYRQ
                     HCYQDWYGSSFISRPYMDLQDKESAVESFKKLKLLWDKRDILVVEGETSRSGVGNDLF
                     NNCNSLKRIICPSKNAYIKYDEILEAVERYAQGRIILLMLGPTAKILVRDLSDRGFQA
                     IDIGHIVSEYEWYKMGATQKIKFSHKHTAEFNDDENINLIDSQNYIDEIVKTITIDDT
                     EFRDTELISIILPVYNMSGSLSRCLDSILRQVYSTFEVILVNHGSTDGSGNICEKYAM
                     KDERIRLFHEEHQVITSVWNFALEKSKGKYVTFVNPDDFLDESYLNYLYNKLRANKSD
                     ISATTYTFFNEEEKCFVYFATDDNYFEKTFFAQEWLEQEHSYHDNLKQIFPLLSMKLF
                     RKSLFNNITFPLEDLTGEYTLYNVYLLSRHITFSHIGLYITSQVFSHTEIKRFTGHDI
                     EKMLSRKEERLALLTMVGFNSEKYIKNYKETLLNYQSESLNIGDINLYNKIKQKIALI
                     DYCY"
     gene            3664..5013
                     /gene="gorA"
                     /locus_tag="VBC74_001230"
     CDS             3664..5013
                     /gene="gorA"
                     /locus_tag="VBC74_001230"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEO6942177.1"
                     /translation="MSKQYDYIVIGGGSAGSGTANRAAMYGAKVLLIEGGQVGGTCVN
                     LGCVPKKIMWYGAQVSETLHKYSSGYGFEANNLSFDFTTLKANRDAYVQRSRQSYAAN
                     FERNGVEKIDGFARFIDNHTIEVNGQQYKAPHITIATGGHPLYPDIIGSELGETSDDF
                     FGWETLPNSILIVGAGYIAAELAGVVNELGVETHLAFRKDHILRGFDDMVTSEVMAEM
                     EKSGISLHANHVPKSLKRDEGGKLIFEAENGKTLVVDRVIWAIGRGPNVDMGLENTDI
                     VLNDKDYIKTDEFENTSVDGVYAIGDVNGKIALTPVAIAAGRRLSERLFNHKDNEKLD
                     YHNVPSVIFTHPVIGTVGLSEAAAIEQFGKDNIKVYTSTFTSMYTAVTSNRQAVKMKL
                     ITLGKEEKVIGLHGVGYGIDEMIQGFSVAIKMGATKADFDDTVAIHPTGSEEFVTMR"
     gene            complement(5056..5508)
                     /locus_tag="VBC74_001231"
     CDS             complement(5056..5508)
                     /locus_tag="VBC74_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942178.1"
                     /translation="MSKGYSRQQKKSTKTNTDSTQSPAKHIKTGFSALQKTIALVGSV
                     LSIIVASITITNAINNHKDSSKTDSSTSKTVIIRESGQNSSNQVSTEKDETRASSSQT
                     LENSSASSTPSSSSNSAANNDQTSSSTNNDQTNNTTTDTSTIENTTTP"
     gene            5760..6905
                     /locus_tag="VBC74_001232"
     CDS             5760..6905
                     /locus_tag="VBC74_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6942179.1"
                     /translation="MIYFDNSATTIPYPEVLRTYQEVASKIYGNPSSLHELGTTSSRI
                     LEASRKQIASLLELKANEIFFTSGGTEADNWVIKGLAFEKQHFGNHIIVSDIEHPAVK
                     ESAKWLGEYGFEIDYAPVDDKGFVDVEALVKLIKPETILISIMAINNEIGSIQPIKAI
                     SDLLSDKPTISFHVDAVQAIGKIPTKDYLTERVDFASFSSHKFHGVRGVGFLYIKEGK
                     RISPLLTGGGQESDLRSTTENVAGIAATAKALRMVMDKEVVAIPKISKMKTIIHDELA
                     KYEDITLFSGKEGFSPNIITFGIKGVRGEVLVHAFEGHDIFISTTSACSSKAGKPAGT
                     LIAMGISTKLAQTAVRISLDDDNDMGQVEQFLTIFKQVYEKTKKVRG"
     gene            6907..8121
                     /gene="thiI"
                     /locus_tag="VBC74_001233"
     CDS             6907..8121
                     /gene="thiI"
                     /locus_tag="VBC74_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEO6942180.1"
                     /translation="MQYSEIMIRYGELSTKKKNRMRFINKLKNNMEHVLSIYPDVSVK
                     TDRDRGHVYLNGTDYHEVAESLKEIFGIQAFSPSFKVEKNVDTLVKAVQEIMTSVYKD
                     GMTFKITAKRSDHSFELDSRALNHTLGDAVFSVLPNIKAQMKQPDINLKVEIRDEAAY
                     ISYEDIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAHDLTRKLTKFGGNIQFIEVPFTEIQEEIKEKAPEAYLMTLTRRFMMRITDRIR
                     EDRNGLVIINGESLGQVASQTLESMQAINAVTATPIIRPVVTMDKLEIIDIAQKIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNTEQYEKRMDVEGLVERAVAGIMVTTIQPQAD
                     SDDVDDLIDDLL"
     gene            8223..9401
                     /locus_tag="VBC74_001234"
     CDS             8223..9401
                     /locus_tag="VBC74_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEO6942181.1"
                     /translation="MARIPYQKTLIFCVAVIIAIFILGLSKDLAQSKGQKVANNNTVK
                     TARVVANGDILLHDVLYASARQPDGTYNFTPYFKEVKSWIESADLAIGDYEGTISSEY
                     PLAGYPLFNAPNEIATTMKETGYDVVDLAHNHILDSQLAGAINTVKTFNRLGLDTIGV
                     YLKDRNKEDILIKRVNGIKIAILGYSYGYNGMEANVSKSDYEKHMSDLDTKKIKQDIK
                     KAEKEADITIVMPQMGIEYQKKPTTEQVILYHSMIKWGADIIFGGHPHVVEPSEVIKK
                     DGQKKFIIYSMGNFISNQRLETVDDIWTERGLLMDVTIEKKGQKTVIKKVKAHPTLVE
                     AKANGRYVEEGFPAYDYRTLVLEDYIEGGKKRSTIDQTMKDKVDKAYKEMNELVNLKW
                     "
     misc_feature    9477..9556
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            9591..9905
                     /gene="rplU"
                     /locus_tag="VBC74_001235"
     CDS             9591..9905
                     /gene="rplU"
                     /locus_tag="VBC74_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEO6942182.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKLDVEAGAEVTFNEVVLVG
                     GETTKVGTPVVEGATVVGTVEKQGKQKKVVSYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            9912..10250
                     /locus_tag="VBC74_001236"
     CDS             9912..10250
                     /locus_tag="VBC74_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEO6942183.1"
                     /translation="MIKATFTRNQSGYLYSAEISGHAGSGEYGFDVICAAVSTLSINF
                     INSLEALTTCQAQLIINDVEGGYMKIDLSSIPQHKEDKVQLLFESYLLGMTNLSKDSS
                     EFVSTVVMTH"
     gene            10272..10565
                     /gene="rpmA"
                     /locus_tag="VBC74_001237"
     CDS             10272..10565
                     /gene="rpmA"
                     /locus_tag="VBC74_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEO6942184.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGANVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            10781..11686
                     /locus_tag="VBC74_001238"
     CDS             10781..11686
                     /locus_tag="VBC74_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6942185.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAAKLFVSQPSLSVAVRDLETELG
                     FQIFTRTTTGAVLTNQGMTFYENALEVVKSFDSFEKQFSQSEATEQEFSIASQHYDFL
                     PPLITAFSKCNDNFSYFRIFESTTIRILDEVAQGNSEIGIIYINSQNKKGLLQRLDKL
                     GLEFVELIPFKTHIYLGKDHPLASKTSLIMTDLEGLPTVRFTQDRDDYRYYSENFVEV
                     LDSSVTYNVTDRATLNGILERTQAYATGSGFLDSRSVNGITVIPLEDHLDNQMIYIKR
                     KDRNLSQMALKFVAVMEEYFKGFNS"
BASE COUNT         3974 a         1673 c         2327 g         3729 t
ORIGIN      
        1 gaactcagaa agtaattgtt caaaagaaaa atacatatca taaaataaag tcaatgttac
       61 ctaaagaaca acaagaaaaa attggttatt taggctttat atatcctaat aaaaagaaca
      121 ataagggtag gaaagatatt tttattttga caaactctga ccaaattgaa catttagaag
      181 tgttggttca tcacttgtct gattatcatt ttcatattgc agcgtacaca gaaatgtctt
      241 ttaagttgat gtcatttagc caagaacaaa atgtaacgct atatccgaat atttcaagaa
      301 ctgatttaga caacttattt gaaatctgtg atatttattt tgacattaat catggaaatg
      361 aagttgatga tgttattaga agggcatttg aatataatca ccttatcttt gcttttgata
      421 atacctgtca taacagagag ttagtattag atagcaatat catttctcac acaacctgtg
      481 aacaattgat aaatttaatg aaaaatttat caggctccat tatgtatttg ctagagcaac
      541 aaagagaaca aacaagtaat gaaacaaaag agcgttataa agaaatatta ggagggtatg
      601 gaaatgccta aaagaaatga attactcaat aaagaaatta aaatgagtat tgataaactt
      661 agatataaag aaccagagag tgaacatgac aagcgaccta ctttttattt ggtagtactt
      721 atacttgtta ctgtagcagt tatattgtcg ttatttaaat attttttata gattcctatt
      781 aatggtagaa aagaagcctg ttgtttttta gataggggaa gtgtgtgagg acatatatta
      841 caaacttgaa tggacattca atcactagta cagcacaaat agctcaaaac atggtaacag
      901 atatagcagt aagcttaggt tttcgtgagc tgggaataca ttcttatccg attgatactg
      961 attctcctga ggaaatgagt aagcgtttag atggaatctg ttccggactt agaaaaaatg
     1021 atattgtcat atttcagaca cctacatgga acactacaac ttttgatgaa aaattatttc
     1081 acaaattaaa aatatttggt gtaaagattg ttatttttat acatgatgtt gtaccgctaa
     1141 tgtttgatgg aaatttttat ttgatggata gaactatagc ttattataat gaagcagatg
     1201 ttttaatagc ccctagtcaa gcaatggtcg ataagcttca aagttatggc ttgacagtaa
     1261 aaaaaatctt agttcaagga atgtgggatc atcccaccaa tattacttta caagcagtta
     1321 atcataagaa gcttgttcat tttcctggga acccagagcg gtttaacttc ataaagaatt
     1381 ggagaattcc taccgagtta catgtttata ctgaccataa catgcaatta cccaccactg
     1441 tggttaaaga gccatatcaa tcggacgaac aattaattat gaaaatgagc gaagggggat
     1501 atggcttagt ctggatggat gaccgtgata aacaatatca atctctttat tgcccttaca
     1561 aactgggcgc ttatatagca gcaggaattc cagtgattat tcaaaaaggt attgctaatc
     1621 aagatataat agaaaaaaat aatttgggat ttattattga aaaaattgat gatattagta
     1681 atatagttga aagtactact gaagaggaat atatggaaat tgttagcgat gtcaggcggt
     1741 ttaatccttt agttagacaa ggatatttta cgcgaaaact attaaccgat gcagtattta
     1801 gtgcattaaa tagcatgtga ggggaatatg aaagaactag aagaaatcgt agttttccct
     1861 ataaacaaaa gtttggatta cattttggaa aatcattcgt cagttattcg ctttggggac
     1921 ggtgaatttg acattataat gggaaaatcg attccttatc aagattacaa cccagagtta
     1981 gcagataggt tattgcaact tcttcataag caaagtacga atgatctatt agtatgtctt
     2041 ccagatatgt ttcaaaatcg acaaagatat aatcaatcgg cacaactttt ttggaaaaat
     2101 cattttcaaa aatttggcaa tttctatagg caacattgtt atcaagattg gtatggttct
     2161 tcttttattt caaggccata tatggactta caagataagg agtctgctgt agaatcattt
     2221 aaaaaattaa agttactttg ggacaaacgc gatattttgg tagttgaagg ggaaacttcg
     2281 cggtcgggtg taggtaatga tctttttaat aattgcaata gtttaaaaag gattatttgt
     2341 ccttctaaaa atgcttatat aaaatatgat gaaattcttg aggctgttga aagatatgct
     2401 caagggagga taatcttatt gatgctaggt ccgactgcaa aaattttagt aagagattta
     2461 tctgatagag gatttcaagc cattgatatc ggtcatattg tttctgaata tgaatggtat
     2521 aagatgggag ctactcaaaa aataaaattt tctcataaac acaccgctga gtttaacgat
     2581 gatgaaaata ttaatttaat tgacagtcag aattatattg atgagattgt taaaacaata
     2641 actatcgatg atactgaatt cagggatact gaattaatat caatcatact tcctgtctat
     2701 aatatgtctg gttctttatc aaggtgttta gattctatcc ttaggcaagt ttactcaact
     2761 tttgaggtga tcttagtcaa ccatggttct acagatggaa gtggtaatat ttgcgagaaa
     2821 tatgcgatga aggatgagcg tattcgttta tttcatgaag aacatcaagt tattacatct
     2881 gtatggaact tcgcattaga gaaatctaaa gggaagtatg ttacttttgt gaatccagat
     2941 gattttcttg atgagtctta cttaaattat ctttacaata aattaagagc taataaatct
     3001 gatatctcag caaccaccta tacttttttt aatgaagagg aaaaatgctt tgtttatttt
     3061 gcaacagatg ataattattt tgagaaaaca ttctttgctc aagaatggtt agagcaagaa
     3121 catagttacc acgataattt aaaacaaata tttcctctcc tttctatgaa gctctttaga
     3181 aaatcacttt ttaacaatat cacttttcct ttagaagatt taacaggaga atacacactg
     3241 tataatgttt atttgctatc aaggcacatt acattttctc atattggttt atacataacc
     3301 tctcaagttt tctctcatac tgaaataaaa agattcacgg gacatgatat agagaagatg
     3361 ctatctagaa aggaagagag gttagccctg ttaacaatgg tagggtttaa ttctgagaaa
     3421 tacatcaaaa attataagga aactttatta aattatcaaa gtgagtcgtt gaatataggt
     3481 gatattaatt tatataataa aattaagcaa aagattgcat tgatagatta ttgttactag
     3541 aaataatttt aacagcaagt taggatatga taatcggtaa tataaattgt ttacgtagca
     3601 gaaaattgtg actgtaaaca ctttgtgata gagtataggt atcaatgatt caaggagaga
     3661 gtaatgtcaa aacaatatga ttatatcgtt attggtggag gtagtgcagg cagtggtacc
     3721 gctaataggg cagccatgta tggagcaaaa gtcctgttaa ttgaaggtgg acaagtaggt
     3781 ggaacttgtg ttaacttagg ttgtgtacct aagaaaatca tgtggtatgg tgcacaagtt
     3841 tctgagacac tccataagta tagttcaggt tatggttttg aagccaataa tcttagtttt
     3901 gattttacta ctctaaaagc taatcgcgat gcttacgtgc agcggtctag acagtcgtat
     3961 gccgctaatt ttgagcgtaa tggggtcgaa aagattgatg gatttgctcg ttttattgat
     4021 aaccatacta ttgaagtgaa tggtcagcaa tataaagctc ctcacattac tattgcaaca
     4081 ggtggacacc ctctttaccc tgatattatt ggaagtgaac ttggtgagac ttctgatgat
     4141 ttttttggat gggagacctt accaaattct atattgattg ttggggcggg ctatatcgcg
     4201 gcagaacttg ctggagtggt taatgaatta ggcgttgaaa cccatcttgc atttagaaaa
     4261 gaccatattc tacgcggatt tgatgacatg gtaacaagtg aggttatggc tgaaatggag
     4321 aaatcaggta tctctttaca tgctaaccat gtacctaaat ctcttaaacg cgatgaaggt
     4381 ggcaagttga tttttgaagc tgaaaatggg aaaacgcttg tcgttgatcg tgtaatatgg
     4441 gctatcggcc gtggaccaaa tgtagacatg ggacttgaaa ataccgatat tgttttaaat
     4501 gataaagatt atatcaaaac agatgaattt gagaatactt ctgtagatgg cgtgtatgct
     4561 attggagatg ttaatgggaa aattgccttg acaccggtag caattgcagc aggtcgtcgc
     4621 ttatcagaaa gactttttaa tcataaagat aacgaaaaat tagattacca taatgtacct
     4681 tcagttattt ttactcaccc tgtaattggg acggtaggac tttcagaagc agcagctatc
     4741 gagcaatttg gaaaagataa tatcaaagtc tatacatcaa cttttacctc tatgtatacg
     4801 gctgttacca gtaatcgcca agcagttaag atgaagctca taaccctagg aaaagaggaa
     4861 aaagttattg ggcttcatgg tgttggttat ggtattgatg aaatgattca aggtttttca
     4921 gttgctatca aaatgggggc tactaaagca gactttgatg atactgttgc tattcaccca
     4981 actggatctg aggaatttgt tacaatgcgc taacgctatc aaagaataca aaaaaagcta
     5041 gggaacctag cttttttaag gtgttgttgt attttcaata gttgaggtat cggtagtagt
     5101 attatttgtt tgatcattgt tagtactaga tgatgtttga tcgttattgg cagcagaatt
     5161 tgaactgcta ctcggagtac tactagcaga ggaattttct agtgtttggc tggatgatgc
     5221 ccttgtctca tctttttcag tagaaacttg gttactagaa ttttgtccac tttctcttat
     5281 aatgacggtt tttgaagtag aagaatcagt cttactagag tctttgtgat tattaatcgc
     5341 attagtaata gtaatagagg caacgataat acttaaaaca ctaccgacta atgcaattgt
     5401 tttttgaagt gcagaaaaac ctgtttttat atgcttagcg ggtgattgag ttgaatcagt
     5461 attagtctta gtagactttt tctgttgtct tgaatatcct tttgacatga cgccctcctt
     5521 aaatgtacat caactattat atgattttat cctaaaaaaa gctaaagaaa aaataattta
     5581 aaacatttaa ataagcaacc taggttatta agctaagcta tgttagtgta ataggtagtg
     5641 agttttgtga taaattatga gaattatcat aaatattgtt ttgggatgtt cacaaactaa
     5701 tataaatagt aggaaaatta tgctataata acactgattg actttagaag gacagattta
     5761 tgatttattt tgataattcg gcaactacta ttccctatcc agaagtttta aggacttatc
     5821 aagaagtagc tagtaaaatc tatggaaacc cctcaagttt acatgagcta ggaacaacat
     5881 cttctcggat tttagaggct agccgtaaac aaattgctag tttacttgag cttaaagcaa
     5941 atgaaatttt ctttacctct ggtggtacag aggccgataa ttgggttatt aagggtcttg
     6001 cgtttgagaa acaacatttt ggaaatcata ttattgtttc tgatatcgaa cacccagctg
     6061 ttaaagagtc tgctaaatgg cttggagagt atgggtttga gattgattat gcgccagtag
     6121 atgataaagg atttgtagac gtagaagcac tggtcaaatt aattaaacca gaaacgattc
     6181 ttatttcaat tatggcaata aacaatgaaa ttggttctat tcagccaata aaagctattt
     6241 cagacttgtt gtcagataaa cctactatat cgtttcatgt tgatgctgtt caagcaatag
     6301 gtaagatacc gactaaagat tatttgactg agcgagttga ttttgcatct ttttctagtc
     6361 ataaatttca cggcgttcgt ggggttggct ttctatatat taaagaagga aaacgtattt
     6421 caccattact aacaggtggt ggacaagaat ctgatttacg ttcaactact gagaatgtcg
     6481 cagggattgc agctactgct aaagctttac gaatggttat ggacaaggaa gtagtagcta
     6541 ttcctaaaat aagtaaaatg aaaaccatta ttcatgatga attagccaaa tatgaggata
     6601 ttacgttatt ttcgggtaag gaaggttttt cacctaacat tatcacgttt ggtatcaagg
     6661 gtgtccgagg agaagttctt gtccatgcct ttgagggaca tgatattttt atctcgacaa
     6721 ctagcgcttg ctcttctaaa gcaggtaaac cagcaggaac tcttatagca atgggaattt
     6781 caactaagtt agcccaaact gctgtacgta tcagcttaga tgatgataat gatatgggac
     6841 aagtagagca atttttaact atttttaaac aagtttatga aaaaactaag aaagttagag
     6901 gataagatgc agtattcaga aattatgatt cgttatggag aactctctac taagaagaaa
     6961 aaccgtatgc gcttcatcaa taagttaaaa aataatatgg agcatgtact ctccatttat
     7021 ccagatgttt cagtaaaaac agatcgtgat agaggacatg tatatctcaa tggtacagat
     7081 tatcatgaag ttgcagagtc cttaaaagag atttttggta tccaagcttt ttctccatct
     7141 tttaaagtag aaaaaaatgt tgatacattg gtaaaagctg tccaggaaat tatgacttcc
     7201 gtttataaag atgggatgac ttttaaaatt accgcaaaac gtagtgacca ctcatttgaa
     7261 ttggatagcc gtgctctaaa tcatacttta ggagatgccg ttttttcagt cttgccaaat
     7321 attaaggctc agatgaagca accagatatc aatcttaaag tcgagatacg agatgaggct
     7381 gcttatattt catatgagga tattaggggt gcaggaggat taccggtagg aacatctggg
     7441 aaagggatgc tgatgttgtc tggtgggatt gattctccgg tagcaggtta cctagcgtta
     7501 aaacgtggtg tagatataga agcagtccat tttgcaagtc ctccttatac tagcccaggt
     7561 gcattgaaaa aagcacatga tttaacacgt aaattgacaa aatttggtgg taatattcaa
     7621 tttattgaag ttccattcac agaaattcaa gaggaaatta aggaaaaagc tcctgaagcc
     7681 tacttgatga cgttaacacg taggtttatg atgcgtatta cagatcgtat tcgtgaggac
     7741 cgaaatggtc ttgttattat taacggtgaa agtttagggc aggtggcaag ccaaacgcta
     7801 gaaagtatgc aagctattaa tgctgtcact gcaacaccga ttattcgtcc tgtggtcacg
     7861 atggataagc tagaaattat tgatattgct caaaaaatag atacttttga tatttcaatt
     7921 caaccatttg aggattgctg tacaattttt gcaccagatc gcccaaaaac taaccctaaa
     7981 attaagaata cagaacagta tgagaaacgt atggatgtag aaggtctggt agagagggca
     8041 gttgcaggga ttatggtaac tactattcaa cctcaagcag atagtgatga tgttgatgac
     8101 ttgattgacg atttattata atgaattttt aaactacaga tttactctgt agttttttat
     8161 taaaatgatt gtgctgtcat aaggttataa aaaatgctat aatacgaata aaggaggcaa
     8221 gtatggcaag aatcccttat caaaaaacac ttattttttg tgtagccgtt attattgcta
     8281 tttttatttt ggggttatct aaggatttag cacaatctaa agggcaaaag gttgcaaata
     8341 ataatactgt aaaaacagca cgtgtggttg caaacggaga tattttgctg cacgatgtac
     8401 tatatgccag tgcgcgccaa cccgacggca cttataattt tactccttat tttaaagagg
     8461 taaaatcttg gattgagagc gctgatttag cgattggtga ttatgaaggg acgattagtt
     8521 cagaatatcc tttagcgggc tatcctttat ttaatgctcc taatgagatt gcaactacta
     8581 tgaaagaaac tggatatgat gtcgttgact tagcacataa tcatattctg gattcacaac
     8641 tggcaggtgc tatcaatact gtcaagactt ttaatcgtct gggattggat actattgggg
     8701 tttatttaaa ggatagaaat aaagaagata tcttaattaa gcgtgtaaat ggtattaaaa
     8761 ttgctatttt aggttattcg tatggctaca atggtatgga agctaatgtt tcaaagagtg
     8821 actatgaaaa acatatgtct gatttagata ctaaaaagat taagcaagat attaaaaagg
     8881 ctgaaaaaga agcagatatc accattgtca tgccacagat ggggatagaa tatcaaaaaa
     8941 aacctactac agaacaagta atactttatc atagtatgat taaatggggg gcagatatta
     9001 tattcggagg acaccctcac gttgttgaac cgtctgaagt gataaaaaaa gatggccaga
     9061 aaaaatttat tatttactct atgggaaatt ttatttcaaa tcagcgttta gaaacggttg
     9121 atgatatctg gacagaaaga ggattattaa tggatgtaac catagagaaa aaaggtcaaa
     9181 aaacagtaat taaaaaagtt aaggcgcatc caacactagt agaagcaaaa gcaaatggcc
     9241 gttatgttga agaaggcttt ccagcttatg attataggac gctagtttta gaggattata
     9301 ttgaaggtgg taagaaacgt tctacaattg atcagactat gaaagataaa gttgataaag
     9361 catataagga aatgaatgag ttagttaatt tgaagtggta aagttgataa ttttattttg
     9421 ttttatcttg cataaaaaaa gagtttatga taaaatttga atgttgacta aatgcacatc
     9481 ctgtgcaacc gcgcgaaaat cgttagatta agtagggcaa ggctctcacg atttataggc
     9541 gagtcttcac agagggaaac ctcacaagaa atttttatag gaggtgcatc atgagcacat
     9601 acgcaatcat caaaactggt ggtaaacaag ttaaagttga agtaggacaa gctatctacg
     9661 ttgaaaaact tgacgttgaa gcaggagcag aagttacatt taacgaagtt gttcttgttg
     9721 gtggtgaaac aactaaagtt ggtactccag ttgttgaagg agctactgtt gttggtactg
     9781 ttgaaaaaca aggcaaacaa aagaaagttg tttcttacaa atacaaacct aaaaaaggta
     9841 gccaccgtaa acaaggtcac cgtcaacctt atacaaaagt tgttatcaac gcaatcaacg
     9901 cttaattttt catgattaaa gcaactttta ctcgtaatca atcaggctat ctttatagtg
     9961 ctgaaatcag cggtcatgct ggtagtggcg agtacggctt tgatgtgatt tgtgcagctg
    10021 ttagtacttt atcaattaat tttattaatt cattagaggc tttaacaacc tgccaagcgc
    10081 aattgattat caatgacgtt gaaggtggtt atatgaagat agatttatct tctataccac
    10141 aacataaaga ggataaggtt caattattat ttgagtctta ccttcttgga atgactaatc
    10201 tgtctaaaga ttcttctgaa tttgttagta cagtagtcat gacacactaa tttaagagag
    10261 gacacaacat tatgttaaaa atgaatcttg ctaacttgca acttttcgcc cacaaaaaag
    10321 gtggaggttc tacatcaaac ggacgtgatt cacaagcaaa acgtcttgga gctaaagcag
    10381 ctgacggaca aacagtatca ggtggatcaa ttctttaccg tcaacgcggt acacacatct
    10441 atccaggtgc taacgttgga cgtggtgggg acgatacact ttttgctaaa gttgaaggtg
    10501 ttgttcgttt cgaacgtaaa ggacgcgata aaaaacaagt gtctgtttac ccaatcgcaa
    10561 aataatgcct agttaagact ttatgactat tcataaggtc ttttattatt gtcagagctt
    10621 tcattttgta acaaaagaca atatgttaag agatttatca ttaatactca taaactttgt
    10681 tgttctttta tagtgaaata gtcctaattc atgaagaatt ttccgcaagg atagttattt
    10741 tttggtataa tgaagggtac taaaatgagg aagtgataaa atgaatattc aacaactgcg
    10801 ttatgttgtt gctatcgcaa atagtggtac ttttcgtgaa gcagctgcaa aactatttgt
    10861 tagtcagcca agtttatctg ttgccgttcg tgatttggaa acagaattag ggtttcagat
    10921 ttttacgcgt acaactactg gtgcagtgtt gaccaatcaa ggcatgactt tttatgagaa
    10981 tgctttagaa gttgtgaaaa gctttgattc attcgaaaaa cagttttcgc aatctgaagc
    11041 tacagagcaa gaattctcaa tagcaagtca acattatgat tttctacctc ctttaatcac
    11101 agcgttttca aaatgtaatg ataattttag ctatttccgt attttcgaat caacaacaat
    11161 acgtatctta gatgaagttg ctcaagggaa tagcgaaata ggtatcattt atatcaatag
    11221 tcaaaataaa aaaggtctac ttcagcggtt ggacaaattg gggttagagt ttgtagagct
    11281 tattcctttt aaaactcata tttatcttgg gaaagaccat cccttggcta gtaaaacatc
    11341 tctgataatg acagatttag aaggattacc aaccgtacgt tttacacagg atagagatga
    11401 ttatcggtat tactcggaga attttgtgga agtacttgat agttctgtta cctataatgt
    11461 tactgataga gcaacattaa atggtatttt agaaagaacc caagcttatg cgacgggctc
    11521 aggattcttg gatagccgca gtgtgaatgg tatcactgtt attccattag aagatcatct
    11581 agataaccaa atgatttata tcaaacgaaa agatcgtaat ttatctcaga tggctttaaa
    11641 atttgttgct gttatggaag agtattttaa gggatttaat tcataggaga agtaatgaga
    11701 aag
//
LOCUS       DATROD010000050        11637 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.050, whole genome shotgun sequence.
ACCESSION   DATROD010000050 DATROD010000000
VERSION     DATROD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11637
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.050"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..928)
                     /locus_tag="VBC74_001239"
     CDS             complement(<1..928)
                     /locus_tag="VBC74_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEO6942186.1"
                     /translation="MKLRKQLAQQIVTSIKDVCQQDINFINTKGIIFASTNPKRVGEF
                     HEIGLKVAQTGQMIEVTDQESYFGTQAGINIPFYYNCELLATIGISGNPNQVGKYALL
                     AQKMTRLILKEHELDYLDFGRKNEASIVLHHLVEGRELDYYYLNQFLNQYHLSEKTDY
                     RLLTFEINSQKQKLLLSQSEMSLLNFFDKLDTAIYTFNYPNQYWLLLSDQMFDYYYPN
                     ILSKFECEKGLYKVGIGQKSSLSLLKRSYETSILALKALKGQQKVNLVDDLDLELLLT
                     SIDSNIKQYVLNKALVNLSENDKILLNSYFKHN"
     gene            1094..2236
                     /locus_tag="VBC74_001240"
     CDS             1094..2236
                     /locus_tag="VBC74_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEO6942187.1"
                     /translation="MKVVVAIDSLKGSLSSLEAGNAIKESINEVIPGADVEVHPLADG
                     GEGTVEALTLGMGGTIEIIPVKGPLGEKVHASYGIIPQRQLAIIEMAAAAGITLIATE
                     ERNPLHTTTYGVGEMINDAISKGCRHFIIGIGGSATNDGGAGMLQALGYALLDKDNQE
                     ISLGAQGLADLKSISTDKVIEELKECDFKIACDVTNPLCGAQGCSSIFGPQKGADEDM
                     ITKMDTWLSNYATLATSVSEKADATIEGTGAAGGLGFAFLAFTNATLEPGIDIILSEI
                     NIEKAISEADLVVTGEGRLDGQTVMGKAPIGVAKLAKKYGKKVVAFSGSVTEDAILCN
                     QHGIDAFFPIVRRLISLDEAMSKEVAYKNMKETATQVFRLINLYNH"
     gene            2261..3517
                     /locus_tag="VBC74_001241"
     CDS             2261..3517
                     /locus_tag="VBC74_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEO6942188.1"
                     /translation="MEATFTTTGALIGLALAILLIIKKVHPAYSLILGALVGGLIGGG
                     DLVTIVNTMVLGAQGMMSSILRILTSGILAGALIKTGSAEKIAESIIKKLGQQRAITA
                     LAIATMIICAVGVFIDIAVITVAPIALAIGKKANLSKSSILLAMIGGGKAGNIISPNP
                     NTIAASEAFKVDLTSLMVQNIIPAIAALVVTIILAKIVSKKNNDISYDSEEQVGSDLP
                     AFLPAISGPLVVICLLALRPLFGITIDPLIALPLGGLISILATGYLKETVPFVEYGLS
                     KVVGVSILLIGTGTLSGIIKASNLQFDMIHLLEFLNMPTFILAPLSGIFMGAATASTT
                     SGTTIASQTFAETLIKSGVPAVSGAAMIHAGATVLDSLPHGSFFHATGGAVNMAIKER
                     MKLISYEALIGLTSTIVAVVYYCFFG"
     gene            3618..4682
                     /locus_tag="VBC74_001242"
     CDS             3618..4682
                     /locus_tag="VBC74_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6942189.1"
                     /translation="MKQTKKEFILGLTIIITLTLLIMLIFLVMFNKSSNSKLKQTKDR
                     RILSTSTTRTSSSSHDQKIVEKSLEKKKMVMEQDMELMNAYRLYYDYAHLSLKEVVLA
                     YMSEYGIEKNSVAFSYKNLKTGQRDAMNDTQAMTAGSTYKLPLNMLVVDAVVKGKLSM
                     EERFDITETDYEYIGEHNNYVAAFSGAMSIDDMQKYSLVYSENTPAYALAERIGGMDS
                     AYSKFGRYGQSKGDIKNIQKNGNKVTTDYYIQVLDYLWKHRKKYNSLITYLEEAFPTD
                     YYRALIPSDVVVAQKPGYVREALNVGAIVKEEVPYIVAIYTAGLGGSTQEDSEINGVG
                     LYQLEQLCFVINQWHRVNMN"
     gene            complement(4738..5073)
                     /locus_tag="VBC74_001243"
     CDS             complement(4738..5073)
                     /locus_tag="VBC74_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEO6942190.1"
                     /translation="MTYLQYVILLVLWEEDHRHMKELSYALSLNSNTLTPVLKRMENR
                     GWIVREKPRDDKRQLIIHLSNKGIESQDAVVNAVQSCGYFFSEISPESYQKTVSALSS
                     LNQVIKSHL"
     gene            complement(5262..6290)
                     /gene="queA"
                     /locus_tag="VBC74_001244"
     CDS             complement(5262..6290)
                     /gene="queA"
                     /locus_tag="VBC74_001244"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEO6942191.1"
                     /translation="MNTNDFDFYLPEELIAQTPLEKRDASKLLVIDHKNKTMTDSHFD
                     HILDELKPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTEGDQWEVLAKPAKRL
                     RVGNRVSFGDGRLIATVTKELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     RDRYQTVYAKENGSAAAPTAGLHFTKELLEKIETKGVKLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSKEAADTLNAVKESGGRIVAVGTTSIRTLETIGSKFNGELKADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGHDFVLEAYNHAVEERYRFFS
                     FGDAMFVK"
     gene            6443..7123
                     /locus_tag="VBC74_001245"
     CDS             6443..7123
                     /locus_tag="VBC74_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEO6942192.1"
                     /translation="MKEAKNFSQQLNILRAGVLGANDGIISVAGVVIGVASATHNLWI
                     IFLSAASAILAGAFSMAGGEYVSVSTQKDTEQAAVAREEKLLENNPELAKKSLVDIYL
                     AKGESHEHAQWLVDKAFSKNAIEHLVEEKYGIEFGEYTSPWHAAISSFIAFAIGSIFP
                     TITILLLPFSVRIVGTVIIVIVSLLSTGYVSAKLGQAPTVPAMRRNVMIGCLTMLATY
                     VIGQLFSI"
     gene            7274..7975
                     /locus_tag="VBC74_001246"
     CDS             7274..7975
                     /locus_tag="VBC74_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEO6942193.1"
                     /translation="MRVITVKNDIEGGKIAFTLLEEKMKTGAQTLGLATGSSPITFYE
                     EIVKSNLDFSNMVSINLDEYVGIAASNDQSYSYFMHKHLFDAKPFKENNLPNGLAKDL
                     KKEIKRYDAVINANPIDFQILGIGRNGHIGFNEPGTPFDITTHVVDLAPSTIEANSRF
                     FNSIDDVPKQALSMGIGSIMKSKTIILVAYGIEKAEAIASMIKGPITEDMPASILQKH
                     DDVVIIVDEAAASKL"
     gene            complement(8047..9003)
                     /locus_tag="VBC74_001247"
     CDS             complement(8047..9003)
                     /locus_tag="VBC74_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEO6942194.1"
                     /translation="MGLLVDGKWVDQWYDTASTGGKFVRTVTQFRHWVTKDGSAGPSG
                     DAGFKAESGRYHLYVSLACPWASRVLIMRKLKNLESHISISIVNPLMLENGWTFQEYK
                     GVIPDMINQSQYLYQIYQASQSDYTGRVTVPVLWDKKCHTIVNNESSEIMRMLNTAFN
                     HITGNTDDYYPDSLQGQIDEMNNFIYPKINNGVYKAGFATSQNVYQKEVETLFTALDQ
                     LEKHLSDNHYLVGEQFTEADIRLFTTLVRFDTVYYGHFKCNLKALHDYPHLWHYTKRI
                     YNLPGIAETINFDHIKKHYYGSHKTINPTGIIPAGPNLDWTI"
     gene            9099..9818
                     /locus_tag="VBC74_001248"
     CDS             9099..9818
                     /locus_tag="VBC74_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO6942195.1"
                     /translation="MRLDKLLGQAGFGSRNQVKKLIRSRQVSVDGQIVTKDNVIVDSG
                     LQSIFVGKERVCLKESSYYLLYKPSGVVSAVRDSEHKTVIDLISEKDKVEGLYPIGRL
                     DRDTEGLLIITNNGPLGYRMLHPKHHVAKTYYVEVNGFLERDAITFFEEGVVFDDGTK
                     CKPAELTIDTANNDKSTARITITEGKFHQVKKMFLAYGVKVIYLRRISFGDLRLDMNL
                     KPGQYRRLRDSEAAILKRYLD"
     gene            9891..11042
                     /locus_tag="VBC74_001249"
     CDS             9891..11042
                     /locus_tag="VBC74_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6942196.1"
                     /translation="MNSKLSLKLTILSISIFLMSHLAIAPAIPKLYAFYHTQNPHIGL
                     ASVESLVTIPAMMITIFVILSNFVVTKLGKKNTVLLGLCLILMSGFISFFTSNFSLAM
                     ASRLLLGIGIGLYNSLSISIITDLYEADERASMIGLRTASLNIGKALTTFIVGLVLAI
                     GVNYIYLVYLLVIPVFFFFWKNVPEVENQTHTLKASTTFDTKAALLMLITFLVGIAYI
                     GATVKIPTLLVTKYHYATSFSSNMLTLLAFSGILVGSVFGKLVKAFQEKTLLIMILAM
                     GIGNVLFALANNQIIFIVASILIGASFVGTMSSVFFYISKNYAKEHNNFITSLALTAG
                     NIGVILTPLILTKLPSQLHLEPFITPFLITSGLMVINVFVYLVLMSKNK"
     gene            11094..>11637
                     /locus_tag="VBC74_001250"
     CDS             11094..>11637
                     /locus_tag="VBC74_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEO6942197.1"
                     /translation="MLIAKDKQGNLINLLESHPGKGQYFCPTCCSAVRLKAGRIMRRH
                     FAHISLKNCQFHHENESNEHLQLKAKLYMSLSRENETMLEHHLPEINQIADLFVNETL
                     ALEVQCSRLSEQRLRERTKAYLQADFQVRWLLGEKLWLKHRLTNLHKQFLQFSQSIGF
                     HIWELDLRLEVLRLKYLIYED"
BASE COUNT         3738 a         1795 c         2242 g         3862 t
ORIGIN      
        1 gattatgctt aaaatatgag ttaagcaaga tcttatcatt ttcagataag tttacaagcg
       61 ctttatttag cacatattgt ttaatattag aatcaatact tgttaataac agttcaagat
      121 caaggtcgtc gacgaggttg accttttgtt gaccttttaa agctttcagg gctaagatag
      181 acgtttcata tgatcttttt aaaagtgata aagatgattt ttgtccgata ccaactttat
      241 agagaccctt ttcgcattca aactttgata ggatatttgg ataataatag tcaaacatct
      301 ggtcagaaag taaaagccag tattgattgg gataattaaa ggtataaata gcagtgtcaa
      361 gtttatcaaa aaagttcaag agtgacattt cagattgact taagagtaat ttttgttttt
      421 gactattgat ttcaaaagtg agtagacgat agtcagtttt ttctgatagg tggtattggt
      481 ttaaaaattg attgaggtaa tagtagtcta actctctgcc ttcaacaaga tggtgtaaga
      541 caatagaagc ttcgtttttt cgcccgaaat ccagataatc taattcatgc tcttttaaga
      601 tgagtcgagt cattttttga gcaagtaaag catattttcc tacttggttg gggtttccac
      661 taatcccaat agtagctaat aattcacaat tataatagaa aggaatattg atccctgctt
      721 gtgtcccaaa ataagattct tgatctgtta cttcaatcat ttgtcctgtc tgtgctactt
      781 ttaaaccaat ttcatggaat tctccaacac gttttggatt ggtactagcg aaaatgattc
      841 ctttggtatt aataaaatta atatcttgtt gacaaacatc tttgatagag gtaacgattt
      901 gttgtgcgag ttgttttctt aatttcattc agcttacctt ttgttatata taacaaatat
      961 catatcataa aaacgctaac atttgttcta tagtctatat tttttgttat ttgattttgt
     1021 tactataatt gagtgatata aaatagtgaa aaaataacaa agattcacaa aatattttca
     1081 aggaggtttt caaatgaagg tagtagtagc tattgattct ttgaaaggga gtctttcttc
     1141 tttagaagct ggcaatgcta taaaggagtc aattaatgaa gtaatcccag gggcggatgt
     1201 ggaagtacat ccattagcag acgggggaga aggaactgtt gaagctttga cgctaggtat
     1261 gggggggacg atcgaaataa tccctgttaa ggggcctctt ggagaaaaag tacatgcgag
     1321 ttatggtatt attccacagc gtcagttagc tattattgaa atggctgcag ctgctggtat
     1381 taccctaatt gctactgaag agagaaatcc tctccacaca accacttatg gagttggaga
     1441 aatgataaat gatgctataa gtaaaggctg taggcacttt attattggta ttggaggtag
     1501 tgctactaat gatggtgggg caggtatgct acaagctttg ggatatgcat tattagataa
     1561 agataatcaa gaaatcagtt tgggagcaca aggattggca gatttaaaat ctatctctac
     1621 tgataaggtt attgaggaat taaaagaatg cgattttaaa attgcatgtg atgtaacaaa
     1681 tcctttatgt ggtgctcaag gttgtagttc gatttttgga ccgcaaaaag gtgctgatga
     1741 agatatgatt acaaaaatgg atacttggtt aagtaattat gctactttag ctacttcagt
     1801 tagtgaaaaa gcagatgcaa ctattgaagg aacaggtgca gcgggtgggc ttggatttgc
     1861 ctttttagcc tttacaaatg ccaccttaga acctggtatt gatattattc tatcagaaat
     1921 taacatagaa aaagcgattt ctgaagctga tttagtagtt actggtgaag gtcgtttgga
     1981 tgggcaaacg gtgatgggaa aagctccaat tggcgtagca aaattagcta aaaaatatgg
     2041 taagaaagta gtggcattct ctggatctgt caccgaagat gctatacttt gtaatcaaca
     2101 tggtattgat gctttttttc caatcgtacg acgccttatc agcttagatg aggctatgtc
     2161 taaagaggta gcttataaaa atatgaaaga aactgctact caagtcttta gattaatcaa
     2221 tttgtataat cattaatatt gaaaggaatt ctgataaatt atggaagcta catttacaac
     2281 aacaggggct ttaataggat tagcgcttgc tattttactg attatcaaaa aagttcatcc
     2341 tgcctatagt ctcattcttg gagcattagt tggtggtttg attggtggcg gtgatttagt
     2401 tacaattgtt aatactatgg tattaggtgc gcaagggatg atgtcatcca tattacgcat
     2461 tttaacgtca gggattttag cgggtgcttt aattaaaacc ggatcagctg aaaaaattgc
     2521 agaaagtatc attaaaaaat taggacaaca gcgagccatc acagctttag ccatcgcaac
     2581 aatgattatt tgtgcagtag gtgtctttat tgatattgca gttattacag ttgctccgat
     2641 tgcattagca ataggtaaaa aagctaatct ttctaaatct agcatactgt tagcaatgat
     2701 tggtggtggt aaggctggga atattatttc acccaatcct aatacaattg ctgcttcaga
     2761 agcatttaag gttgatttaa caagcttaat ggtacaaaat attattcctg caattgcagc
     2821 tttagtagtt acgattatat tagcaaagat tgtttccaag aaaaataacg atatttctta
     2881 tgatagtgag gagcaagtgg gctctgatct gccagcattc ttaccggcta tttctggtcc
     2941 acttgtggtt atctgcttat tagctcttcg tcctttattt ggaatcacta ttgatccctt
     3001 gattgcactg ccgttgggtg gtctaattag tattttagca acaggttacc taaaagaaac
     3061 ggtaccgttt gttgaatacg gtttatcaaa agttgttggc gtttcaattc ttttgattgg
     3121 aacaggtaca ctttcaggta ttattaaagc atctaatcta caatttgata tgattcatct
     3181 ccttgaattt ttaaatatgc cgacctttat ccttgcccca ctctcaggta tttttatggg
     3241 agcagcaaca gcatcaacga cttctggaac aactattgct tcacaaacgt ttgctgaaac
     3301 cttgattaaa tcaggggtac cggcggtttc gggtgcagct atgattcacg caggtgcgac
     3361 tgttctggat tcgctacctc atggatcctt cttccatgcg acaggcggag ctgtcaatat
     3421 ggctataaaa gaacgtatga aactaattag ctatgaagct ttgattggat taactagcac
     3481 aatagttgct gtcgtttact attgtttctt tgggtaaaat ttggatgaat acgtgatgtt
     3541 tattatcact gtttgaatag aattctcatt gattttttct aattttctta ctataataaa
     3601 ttagtaggag aggtgttgtg aaacaaacaa agaaagaatt tattttagga ttaaccatta
     3661 ttattaccct tactttatta atcatgctga tttttttagt aatgtttaat aagtccagta
     3721 actctaagtt gaagcagaca aaagatagaa gaatattatc tacttcaacc acaaggacat
     3781 ccagtagctc acatgatcaa aaaattgtag aaaagtctct tgaaaaaaag aaaatggtca
     3841 tggaacaaga tatggaattg atgaatgcct ataggctcta ttatgattat gcccatttgt
     3901 ccttgaaaga ggttgttctg gcctatatgt ctgaatatgg cattgaaaaa aatagtgttg
     3961 ctttttctta taaaaattta aaaacaggac aaagggacgc tatgaatgat actcaggcta
     4021 tgacagcagg gagcacctat aagttacctc ttaacatgtt ggtagtagat gcagtggtca
     4081 agggcaagtt gtccatggaa gaacgctttg atattaccga gactgactat gaatatattg
     4141 gtgaacataa taattatgtt gctgctttct cgggggcaat gtctattgat gatatgcaaa
     4201 aatactcttt agtttattca gaaaatacac ccgcctatgc tttagctgag cgtattggtg
     4261 gcatggatag tgcttattca aagtttggtc gttatggcca atctaaagga gacatcaaaa
     4321 atatacaaaa aaacggtaat aaagtgacaa cagattacta tattcaagtg ctagactatc
     4381 tctggaaaca tcgtaaaaag tataatagtt taattactta tttagaagaa gcttttccaa
     4441 cggattatta tcgtgctttg ataccatctg atgttgttgt tgctcaaaag ccagggtatg
     4501 tccgtgaggc gttgaatgtt ggagcaattg tcaaagaaga agtgccttat atcgttgcta
     4561 tttatactgc aggtttaggt ggctctactc aggaagattc agaaattaat ggagtgggac
     4621 tatatcagct agaacaatta tgttttgtta ttaatcaatg gcatcgtgtt aatatgaatt
     4681 agaaataaat ctatcaaaat aataaaaacc tcttagatta actatctaag aggtttttta
     4741 taaatgtgat tttataactt gattaagtga agaaagggca gaaacagttt tttgataaga
     4801 ttcaggacta atttctgaga aaaaataccc gcacgattgc accgcattga caacggcatc
     4861 ttgtgactca atacctttgt tacttagatg aataatgagt tgccttttgt catctcttgg
     4921 tttttctcgt acaatccacc cacgattttc catacgcttt aaaactggag ttagggtatt
     4981 cgaattaaga gatagtgcat aactaagttc cttcatatgc cgatgatcct cttcccaaag
     5041 gactaacaag ataacgtatt ggagataagt caattgaaat tcatccaaag ctttttggta
     5101 aaatttattg aaaagtcgat gtgtttcact aaccgtaaaa cataatcgct cgtctaaaat
     5161 acttcgatta ctcattatca tattgtagca ttttggaggc gttatagtat gtcaatcgtg
     5221 ttaaaaaacg ataaaacttt ttcctaattg aaagcctatt cttacttaac aaacatagca
     5281 tcaccaaaac tgaagaaacg atatcgttct tcaactgcat ggttgtaagc ttctagtaca
     5341 aagtcatgtc cagcaaatgc tgatactaac atgacaagag tagactttgg taagtgaaag
     5401 ttggttgaaa aagcgtctac aaccttgaac tgataaccag gcttgataaa aatatttgtc
     5461 caccctgagt cggcttttaa ttcaccattg aatttagaac caattgtctc taatgtgcga
     5521 atagaagttg taccaacagc tacaatacgt ccaccagatt ctttaactgc attgagagta
     5581 tcagcagctt ctttagataa ttggtaaaac tcagaatgca tttcgtgttc atcaagatta
     5641 tcaactgaaa caggacggaa agttccaagt ccaacgtgaa gcgtcaaata gacaagcttc
     5701 acaccttttg tttcaatttt ttccaatagt tcttttgtaa aatgaagacc ggctgttggt
     5761 gcagcagcag aaccattttc ttttgcatac acagtctgat agcggtcacg atcttctagt
     5821 ttttcatgaa tatatggcgg aagtggcatt tcgcccaaac tttcaaggac ttctagaaaa
     5881 ataccatcat atgagaattc tacaatacgc ccaccatgtt ctaattcctt tgtgactgtt
     5941 gctattaacc gaccatcacc aaatgaaact ctgttcccta cgcgtaagcg tttagcgggt
     6001 ttagcaagca cttcccattg atcgccctct gtatttttga gaagtaataa ctctacatgg
     6061 ccgtgtgtat ctggcttttc accataaagg cgagcgggca gaacacgtgt gttattcatt
     6121 actaaagcgt caccaggctt gagttcatct aagatatgat cgaaatggct atctgtcatc
     6181 gtcttattct tatgatcaat gactaatagt ttagaagcgt ctcttttttc aagtggtgtt
     6241 tgggcaataa gttcttctgg taaataaaaa tcaaaatcgt ttgtattcat gatttctcct
     6301 atctataagt aagcttttct attatatcac gttcggtaaa aatgacccaa ctaatcttct
     6361 tatttagaat gattataaaa aataattgta atcattctaa ataagtggta taataattgt
     6421 cagaaagtga gaagtagaga agatgaaaga agcaaaaaat ttttcccaac aattgaatat
     6481 tttaagagct ggtgttcttg gtgctaatga tggtatcatc tctgtagctg gtgttgttat
     6541 tggtgttgcg agtgcaactc ataacctttg gattattttt ttatcagcag cttccgcgat
     6601 tttagctggt gcattttcta tggctggcgg ggagtatgtt agtgtttcta ctcaaaaaga
     6661 tactgaacaa gcagcagttg cgcgtgaaga gaaactacta gaaaataatc cagaacttgc
     6721 caaaaaatcc ttagttgata tctacttagc aaaaggagaa agtcacgaac atgctcaatg
     6781 gctagttgac aaggcttttt caaagaatgc tattgagcat ttagttgaag aaaaatatgg
     6841 tattgagttc ggtgaatata caagcccctg gcatgctgct atatctagtt ttatagcttt
     6901 tgccatcgga tcaatttttc caactataac tattctgtta ttaccatttt cggtacgaat
     6961 tgtaggaacg gtaataattg ttattgtttc actattatca actggttatg taagtgctaa
     7021 gttaggccaa gcccctacag tacctgccat gcgtcgaaat gtgatgattg gttgtttgac
     7081 aatgttggca acctatgtca ttggacaatt attttcaata taaaatatta atcgtggagt
     7141 tattgtctaa actagaatgg acagtaattc tttttagttt tataacgaaa ttaagaagtt
     7201 tttattgaca aaaattggta tataccatat aatataatca taaaggtcta taccaaataa
     7261 aaggagagta aacatgagag ttattacagt taagaacgac attgaggggg gaaaaattgc
     7321 atttactctt ttagaagaga aaatgaaaac tggtgctcaa actttaggat tggcaacagg
     7381 aagtagccca atcacctttt acgaagagat tgtaaaaagt aaccttgatt tttcaaatat
     7441 ggtttctatt aaccttgatg aatatgttgg aattgccgca agtaatgatc aaagttactc
     7501 ttattttatg cataaacatc tttttgatgc taaacctttt aaggaaaata accttccaaa
     7561 cggcttggct aaagatttga aaaaagaaat aaaacgatac gatgctgtca taaatgctaa
     7621 tccaattgat tttcaaattc ttggaattgg tcgtaacggt catattggtt ttaatgaacc
     7681 aggtacacct tttgatatta caacacatgt ggttgactta gctccaagta caattgaagc
     7741 taatagtcgt ttctttaata gtattgatga tgtaccaaag caagccttat caatgggtat
     7801 aggttctatt atgaaatcaa aaacaatcat attagtggcg tatggtattg aaaaagcaga
     7861 agcgattgca agtatgatta aaggacctat cacagaagat atgccagcaa gtatcctcca
     7921 aaaacatgat gatgttgtga ttattgtgga tgaagcagcg gcatcaaagt tgtaaagtaa
     7981 ataataaaaa agtaaggatg aatctcaatg agaaaaaatt cattcttact tttttattat
     8041 gctttattaa attgtccagt ctaagttagg tccagctgga ataataccgg tagggttaat
     8101 ggttttatgg ctaccgtaat agtgtttttt gatatgatca aaattaattg tctcagcaat
     8161 tccaggtagg ttgtaaatac gttttgtata atgccataaa tgtgggtaat catgtagcgc
     8221 ttttaaatta catttaaaat gaccataata aacggtgtca aagcgaacta aagttgtgaa
     8281 aaggcgaata tcagcttcgg taaattgttc tccaacaaga taatggttat cagataagtg
     8341 tttttctaac tgatctaaag ctgtgaataa ggtttcaact tccttttgat aaacgttctg
     8401 agaagtagca aaaccagctt tatagacacc attatttatt tttggataaa taaaattatt
     8461 catttcgtca atttgtcctt gtaaggaatc gggatagtaa tcgtcagtat ttccagtaat
     8521 atgattaaat gcagtattta acatgcgcat aatttcagat gattcattgt taacgattgt
     8581 atggcatttt ttatcccaaa gaacagggac tgtcacgcgt cctgtataat cactttgact
     8641 tgcttgatag atttggtaaa ggtattggct ttggttaatc atatcaggaa taacgccttt
     8701 atattcttga aaagtccaac cattctccaa cattaaaggg ttaacaatgg agatagagat
     8761 atgagattct agatttttaa gctttctcat gattaataca cgtgatgccc aagggcaggc
     8821 tagtgaaacg tagagatgat atcgtccaga ttctgcttta aaacctgcat ccccactagg
     8881 ccctgctgaa ccatctttag taacccaatg acggaattgt gtaacggttc ggacgaattt
     8941 accaccagtt gatgcagtat cataccattg atcaacccat ttgccatcta ctaaaagtcc
     9001 catgataatc tcctttgttt ttctattact aattagtagt ataaagattt taaaaggaat
     9061 tagcaaatgt ttaacttaca gtatggtaaa ctaagggtat gcgtttagat aaattattag
     9121 gtcaggcagg atttgggtct agaaatcagg ttaagaaatt aattcgttct aggcaagttt
     9181 ctgtagatgg tcaaattgtt acaaaggata atgttattgt agatagtggt ctacaatcga
     9241 tttttgtagg aaaagagaga gtctgcctaa aagaaagttc ttattacctt ctttataagc
     9301 catcaggagt tgtttcagca gttagagatt cagaacataa aacagtcata gacttaatta
     9361 gcgaaaaaga taaggtggag ggattgtatc cgattggtcg tctagatcgt gatacggaag
     9421 ggcttttaat aatcacgaat aatggtccac tgggttaccg aatgttacat ccaaaacacc
     9481 acgttgcaaa aacgtattat gttgaagtaa atggtttctt ggaaagagat gctattacct
     9541 tttttgaaga aggagttgta tttgacgatg gaacaaagtg taaaccagct gaattaacga
     9601 ttgatacggc taataatgac aaaagtaccg caagaataac tatcacagaa gggaaatttc
     9661 accaagtaaa aaagatgttt cttgcatatg gcgttaaagt tatatacttg aggcgtattt
     9721 cttttggtga cttgaggctt gatatgaatt tgaaaccagg tcagtatcgt agattgagag
     9781 attcagaggc agctatctta aaaaggtatt tagattagtg aaacgactca cttgaccttg
     9841 cttagtattc ttcatatact tataagtgat tattttaaag gagaaaacta ttgaattcaa
     9901 aactttcatt gaaattgact attttatcga tttctatctt tttgatgtct cacttagcaa
     9961 ttgctcctgc aattcctaaa ttgtatgcct tttaccatac tcaaaaccct catattggac
    10021 tagcttcagt agagagttta gttactattc cagctatgat gattactatt tttgttattt
    10081 tgagtaattt tgtagtcaca aaacttggca aaaaaaacac ggtcttactt ggtttatgtt
    10141 taatcttaat gtctgggttt atctcatttt ttacaagtaa tttttcactt gcgatggcta
    10201 gtcgactact tttgggtatt ggtattggtc tctataattc gctatcaatt agtatcataa
    10261 cggatttata tgaggctgat gagagagcca gtatgattgg tcttagaaca gcatcactta
    10321 atatcggtaa agccttaact acatttattg ttggattggt tttagctatt ggtgtgaatt
    10381 atatttattt ggtttacctt ctggttattc ctgttttctt tttcttttgg aaaaatgttc
    10441 cagaggttga aaatcaaaca cataccctta aagctagtac aacatttgat actaaagcgg
    10501 ctttgttgat gctcatcacg ttcttagtag gtattgctta tataggagca actgtcaaaa
    10561 ttccaacctt actagtgaca aaatatcact acgcgacgag cttctctagt aatatgttaa
    10621 ctctccttgc ttttagtggt attttagttg gatctgtatt tggaaaatta gtcaaagcat
    10681 tccaagagaa gacgctatta atcatgattc tagctatggg aataggtaat gtgctatttg
    10741 ctttagctaa taatcagatc atttttatcg tagctagtat tttaatagga gctagttttg
    10801 ttggcaccat gtcttctgta ttcttttata tttcaaaaaa ctatgcaaaa gaacataata
    10861 actttatcac aagccttgcc ttaacagctg gtaatatagg agttattttg acaccgctta
    10921 ttttaacgaa acttccaagc cagctacatc tggaaccttt tataacacca tttttaataa
    10981 caagtgggct gatggtgatt aatgtctttg tttacctagt tttaatgtca aaaaataaat
    11041 aaaaagttat ccgaaaggat agcttttttc gaatagtaaa agtaggagaa aaaatgttaa
    11101 ttgctaaaga caaacaagga aatttaatta atctactcga gtctcatccg ggaaagggac
    11161 agtatttttg tccaacttgt tgttcagctg taaggttaaa agcaggtcga atcatgagaa
    11221 gacactttgc acatattagt ctaaaaaatt gtcagtttca tcatgaaaat gagtctaatg
    11281 agcacttgca attaaaagca aagttatata tgagtttaag tagagagaat gaaactatgc
    11341 tcgagcatca tttgccagaa attaatcaga ttgcagattt atttgtgaat gagacattgg
    11401 ctttagaagt tcagtgtagc cgactatcag agcagcgact cagggagcga acaaaagcat
    11461 atctacaggc agactttcaa gtgagatggt tactagggga aaaactctgg ttaaagcatc
    11521 gtttaacaaa tctacataaa caatttttac aattttctca aagtatcgga tttcatattt
    11581 gggagttaga tttgaggtta gaagtactga gactaaaata tttgatttac gaagatt
//
LOCUS       DATROD010000051        11618 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.051, whole genome shotgun sequence.
ACCESSION   DATROD010000051 DATROD010000000
VERSION     DATROD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11618
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.051"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            67..342
                     /locus_tag="VBC74_001251"
     CDS             67..342
                     /locus_tag="VBC74_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEO6942198.1"
                     /translation="MRTEKDIENYLKKKTKGLCLKFTSPGTVGVPDRIVVMKTGVFFV
                     EVKAPGKKPRPSQILMHKKIKEVGQPVWVVDSYESVDIALKEMENWV"
     gene            339..1661
                     /locus_tag="VBC74_001252"
     CDS             339..1661
                     /locus_tag="VBC74_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEO6942199.1"
                     /translation="MRLHEYQEYAKTWIVEHPYCGLLLDMGLGKTLTTLSAIDEIQNI
                     FSEDHKILIVAPKKVAEETWPTEIGKWHFDFTYSKVLGSEGKRIEALETEADIYLINR
                     ENVTWLVEYYKTKWPFTFVVIDELSSFKSSKSKRFRALRKVRPKVQRLVGLTGTPAPN
                     SLIDLWPQIYLMDRGDRLETSQTRFKDKYFVPDKRNGPIIYSWALRDGAEAEIYNKIE
                     DICVSMKAKDYLKLPPRTDNVVSVKLSNMKAYKQFEADLVLEFKNKEISAANSAVLAN
                     KLLQMANGAIYDDDKATVAIHDDKLDALENIVEESQGQPILVFYQYQHDLERLKKRFP
                     QAEELTSVDKWNSEKIPILLCHPQSAGHGLNLQKGGHIIVWFGLTWSLEYYQQANARL
                     DRQGQTEPVIVHHIVAENTVDEKVLRILQGKEKNQNALLEAVKAQLGV"
     gene            1662..1832
                     /locus_tag="VBC74_001253"
     CDS             1662..1832
                     /locus_tag="VBC74_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942200.1"
                     /translation="MKKEYVVRIYTGREKNLEAKPQFEEKTFTRKADMLKFWNSCEAT
                     VKEKYTREREND"
     gene            2114..2308
                     /locus_tag="VBC74_001254"
     CDS             2114..2308
                     /locus_tag="VBC74_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942201.1"
                     /translation="MKIEFFLKQGPTKSVSNYGEVGIDEVIGAIKNNDIVALNGKNNA
                     VIIPKQNISHVIIHKEVTNE"
     gene            2301..2717
                     /locus_tag="VBC74_001255"
     CDS             2301..2717
                     /locus_tag="VBC74_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF722 domain-containing protein"
                     /protein_id="HEO6942202.1"
                     /translation="MNKLSNAQLKAFDEWLFDYRFIDNKIALRKLELQTDMNTEVDKN
                     IGGGRANFVSKVTEDVVARWDSDRELKGLSNFKEAVIATLNLLDEELTDIFNLRWGVG
                     SSNTWEEIAYTRHLSRKSIYRKRERILEIFSKKIGM"
     gene            2807..3259
                     /locus_tag="VBC74_001256"
     CDS             2807..3259
                     /locus_tag="VBC74_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEO6942203.1"
                     /translation="MLGGDGKLSKLTLKQKRFADEYIISANATAAAIKAGYSKKTARS
                     IGQENLTKPDIKAYIDERLEKLESEKIATQEEVLQYLTSIMRGDQQEKTLISVGEFGQ
                     KIVDIDVGAKDRIKAAELLGKRYRLFTDKVEMDVSSDITISVGEWDDD"
     gene            3249..4526
                     /locus_tag="VBC74_001257"
     CDS             3249..4526
                     /locus_tag="VBC74_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEO6942204.1"
                     /translation="MTIKQRPKINIVIKHPSKVFNKHIYDKLYNYSNFTEVHYGGASS
                     GKSHGVFQKIILKALNPKFKHPRKILVLRKVGATVRDSVFADIMSNLSYFGVLDKCKI
                     NMSAFRITLPNGSEFIFKGMDNPEKIKSIKGISDVVMEEASEFTLDDYTQLTLRLRDK
                     KHLEKQVYLMFNPVSKANWVYKAFFIKSPKNTVVYQTTYKDNRFLDDVTRENIEELAD
                     RNEAYYKIYALGQFATLDKLIFPKYDKQILNKDKLSHLPSFFGLDYGFINDPSAFLHV
                     KIDDANKKLYILEEYVRKNLTNDKIANAIKDLGYAKEEIRGDSAEKKSNQELRNLGIP
                     RMIDVTKGPGTVMQGIQYLLQYDWIVDERCVKTIEELENYTWKKDKKTNEYINEPVDS
                     YNHCIDAIRYAVQDRIYQSADRSKRMKNAKYYF"
     gene            4542..6074
                     /locus_tag="VBC74_001258"
     CDS             4542..6074
                     /locus_tag="VBC74_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEO6942205.1"
                     /translation="MEEKQFLVGTRFNENANKQFIMLQEDFEAIDYESKLWIDQLKNY
                     ISRFKAEQLERLKELKRYYLGDNNIKYRPAKTDKYAADNRIASDFAKYITVFEQGYML
                     GVPVEYKNENKDLQAAIDLMSVRNNEDYHNVKIKTDLSIYGRAYELLTVEEIDDKKTE
                     VKLYQLPAEQTFVIYDDTYQRNSLMAVHFYDIDYGSGKRKQIIKVYTSDTIYTYEDYN
                     FETKGMRLKEHEEHFFKGVPVNEYANNEERTGAYESVLDNIDAYDLSQSELANFQQDS
                     VNALLVIAGNTYTGADENDYLDDGRLNPNGRLAISIGFKKAQVLILDDNPNPNGVKPQ
                     AYFLKKEYDTAGSEAYKNRLVADILRFTFTPDTQDMKFSGVQSGESMKYKLMASDNYR
                     EKQERLFKKGLMRRLRLAANIWAIKGNEATTYSLVNDTSIVFTPNLPQNDNEIVTAAQ
                     NLYGIVSDQTIFEILNTVTGVDAEAELERLKEEADKKQSLPEPRLVGDASGQEEPTAE
                     KP"
     gene            6034..7482
                     /locus_tag="VBC74_001259"
     CDS             6034..7482
                     /locus_tag="VBC74_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEO6942206.1"
                     /translation="MLVVKKNQQQKSLNYWQKRQEDILSYLDRTDLDVFSELQKLYNE
                     QAFELQKELFDFYTKYSEENKMTYQDVVKKLRHEDLSDYVANANKYRKQAEKDPELLK
                     RLNEQYVSARATRMDALNLELVYRAGILKGVLDSVFENHLKKAASYAYKKAMGGRSGT
                     INGPVLEELVRTPFDGYNYSEQLWGNTDNLVKNLQKKLKQGFVRGEHPRAIARDLAKQ
                     FNVANHRAETLIRTDGTMVINNATARRYLNAGLKYYRDLVRLDDRTTEICRKIAKENK
                     RKLLSELKPGINAAPYHFNCRTTIIPDEDELSIEVDPIDDKSTKYFKDVTSDWIDGNE
                     HKPQLSLLNEYVKNGTPYKVDGHNVVLDHSNYEYRVANWLSKKTGLQVDMVPRVNSPE
                     HINTPDYLVDGVPFDLKEITGSGKNVIDGNLRKAKKQATNIIFDITKTPLSFEEIMGQ
                     LEHIYMLDRRGLDISIIKNKDEVLAVLEKEGR"
     gene            7510..7698
                     /locus_tag="VBC74_001260"
     CDS             7510..7698
                     /locus_tag="VBC74_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942207.1"
                     /translation="MGVRPSSFFISIIHQPIKTIKEENMFIWQMVLVALGVLVLILIV
                     GIAAIAVKSIIAELKKVG"
     gene            7701..7967
                     /locus_tag="VBC74_001261"
     CDS             7701..7967
                     /locus_tag="VBC74_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942208.1"
                     /translation="MNKRIKKKRKLETAVVMLVAENAMQAEALRNQNKQITELRAIVQ
                     QNAQATNEEFASVKAATLDNQSAIADIAIDVNYIKKNYKRKWGK"
     gene            8123..8698
                     /locus_tag="VBC74_001262"
     CDS             8123..8698
                     /locus_tag="VBC74_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEO6942209.1"
                     /translation="MAEENVTTETTEQVDTQQETVEHPKQEHERTFTRADISKMMTAE
                     RAKWEAEQSEAIEKARTEGERLAKLSKDERAKEEEQKRLSAIAEREKAVAEREMRIET
                     QSLLAEKGLPLDFIDVVLATTADEVKTNIDNLQSIFDQAVENRVNERLTQKSPRTGNG
                     SVGMTKAEIMAIENDDERLKAIAANRNLFTR"
     gene            8717..9601
                     /locus_tag="VBC74_001263"
     CDS             8717..9601
                     /locus_tag="VBC74_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEO6942210.1"
                     /translation="MAENNLNVMSDLGEIKSIDFVNKFSTNINELLKLLGVTRKQELT
                     NDLKIQTYKWSTTLDKTNPAEGETIPLSKVTRAKAKEYTVEWFKKRRATTAEAIARHG
                     VARAINEADTRIMREIQNGFKTNFFEFLKKSTTKVTGTTLQSALSQSWAKIATFNEFD
                     GSPIISFVSPLDVAKYLGDTNVGADASNVFGMTLLKNFLGMQNVIVLPSVPEGKVYTT
                     AVENLVFAYLNVNSGDLGGMFADFTDETGLIGAARTRQLSNLTYESVFFGANVLFAEI
                     PEGVVEATIEAPKVVPGG"
     gene            9605..9961
                     /locus_tag="VBC74_001264"
     CDS             9605..9961
                     /locus_tag="VBC74_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEO6942211.1"
                     /translation="MDKELLLTEVKLFKGISAKDTSQDDLIKLSINESIDRILAKLND
                     YSDTEMTEIPKRLTFIVRDVAIKRYNRLNSEGTVEDSEEGKTFKWDSYLKEYESTLRS
                     AAIGKVYSGKGVARFI"
     gene            9972..10250
                     /locus_tag="VBC74_001265"
     CDS             9972..10250
                     /locus_tag="VBC74_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942212.1"
                     /translation="MIYKDRVILVYVDEQDDFLDKRTVEKPSGKIPCMENTFTKSEQM
                     GLFGKYDLNAFKLHLQGRYDGFSKIIYKGKPRLIKGLAHHKNSTVIYV"
     gene            10247..10591
                     /locus_tag="VBC74_001266"
     CDS             10247..10591
                     /locus_tag="VBC74_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEO6942213.1"
                     /translation="MSLIYRMRGLDRFLRSVERKQKSVRIAVDKELSKSAARIERQAK
                     ILAPVDTGWLRAQIYSEQQRLLHYRVVSPALYSIYLELGTRKMEAQPFLDPALRKEWP
                     VLMANLKKMFKR"
     gene            10595..10954
                     /locus_tag="VBC74_001267"
     CDS             10595..10954
                     /locus_tag="VBC74_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942214.1"
                     /translation="MDYSPETLYLKKVKNRLGVLDIPIYFKLPKSDVLEPFIVVGTNI
                     SDLSKTAQTGAVIDDFSLNIDAFLPGDSRLDAEEIKSRMLRLLGRNNQIKAQILVDNS
                     IGREVYRVAINITEILI"
     gene            10966..11598
                     /locus_tag="VBC74_001268"
     CDS             10966..11598
                     /locus_tag="VBC74_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEO6942215.1"
                     /translation="METTVEITTAKPIKGKNIFYFIQSVDAELGSKAILPAYRTDGTT
                     KLGGDFIDEQTQQGRVLEKATDEHSIDLTSYYTPKDKAIEIVEIAKTTGKSVKVWRVI
                     ADESLKEKGSGTLEGKEVYPAKFGYGKIDEIEYSEGVEKLVEASYTLNIIDSLKDGKF
                     PLTAEDIALLTSVYAYQNPGETTGDYDNIQKKEVSGSLLGAVPGISSEGH"
BASE COUNT         4112 a         1867 c         2471 g         3168 t
ORIGIN      
        1 ataaaaaaga gaaagtaagt tgaaaaaaaa cggtaaccct gacctttatt gaccaaagga
       61 ggaaggatga gaactgaaaa agacattgaa aattatttga aaaagaaaac aaaaggcctg
      121 tgtttaaaat ttaccagtcc agggacggta ggagttccgg acagaattgt tgtcatgaaa
      181 actggtgtat tttttgtaga ggtcaaagcg cctggtaaaa agccaagacc gagtcaaatc
      241 ctgatgcaca aaaaaataaa ggaggtaggt caaccagttt gggttgttga ctcctacgaa
      301 tcagtggaca tagccttaaa agaaatggag aactgggtgt gagactgcat gagtatcaag
      361 aatacgctaa gacatggata gtagagcacc cttattgtgg ccttttactt gacatgggcc
      421 ttggtaaaac gctgacaaca ctatcggcaa tagatgagat tcaaaatatt ttttccgagg
      481 atcataagat tttaatcgta gctcctaaaa aagtggcgga agaaacgtgg ccaacggaga
      541 ttgggaaatg gcattttgat ttcacctatt ctaaagtttt ggggagtgag ggaaaacgaa
      601 ttgaagcctt agaaacagaa gccgatatct atttgattaa tcgtgagaat gttacttggc
      661 ttgttgaata ctacaagact aaatggccgt ttacctttgt tgttattgat gagctgtcaa
      721 gctttaagtc tagtaagtca aaacggttta gggctttgcg aaaagttaga ccgaaagtcc
      781 aacgccttgt agggctaaca ggaaccccag cgcctaacag tttgattgat ttgtggccgc
      841 agatttatct gatggacaga ggcgacaggc ttgagacgag ccagactcga tttaaagaca
      901 agtattttgt tcctgataag cgtaatggtc caatcattta cagttgggca cttagggatg
      961 gtgcagaagc agaaatctat aacaagattg aggacatctg tgtcagcatg aaagctaaag
     1021 actatctcaa gttaccgccg cgaaccgaca acgttgtatc agttaagtta tctaatatga
     1081 aagcctacaa acagtttgaa gctgatttgg tgttggagtt taaaaataaa gaaatatctg
     1141 cggctaattc tgcggttttg gccaataaat tacttcaaat ggccaatggt gctatctatg
     1201 atgatgataa agcaacagtt gctatacacg acgacaaact tgacgcgctt gagaacatag
     1261 tagaagaaag ccaaggccag cctatcttag ttttttacca gtatcaacat gatcttgaga
     1321 gacttaagaa acgatttcct caggctgaag agctgacgtc ggttgacaag tggaattccg
     1381 aaaaaatacc aattcttctg tgccaccctc aatcggcggg gcatgggctt aatctacaaa
     1441 aaggcgggca tattattgtt tggtttgggc taacatggag tcttgaatat tatcagcaag
     1501 ctaatgccag attagataga caggggcaga cagaacccgt tattgtgcac cacattgttg
     1561 cagaaaatac agttgatgaa aaagtactta ggattttaca aggcaaagaa aaaaatcaga
     1621 acgccttact tgaagcagtt aaggcgcagt taggagtcta gatgaaaaaa gaatatgtcg
     1681 ttagaatcta cacgggtaga gaaaagaatc ttgaggcaaa acctcagttt gaagagaaaa
     1741 cctttacgcg aaaagcggac atgttgaagt tttggaattc ttgcgaagca acagttaaag
     1801 aaaaatatac tagggagcgg gagaatgatt gaagaacaat aaatacttga ctgcttagtc
     1861 gacaaaatgt ttcagcagga cacaaaagca acttaaacgt gctagcgagc aaaatcagag
     1921 acagacaaag cggattgctg atttgactgg aaatgggggg taggtatgaa tataatccat
     1981 agaatggaaa acgggataaa cgctgcaggt gatttaataa cttatgcagt accatgttgt
     2041 ccagagtgta aaagtgaaac atatagtcag aaacactgtc ctttttgcgg tgaaaatctt
     2101 atggaggtag caaatgaaaa ttgaattttt tttaaagcaa ggtcctacta agagcgtttc
     2161 taattatggt gaagttggta tcgatgaagt aattggtgca ataaaaaata acgatatcgt
     2221 tgcgctaaat ggaaaaaaca acgcagttat tatacccaaa caaaatattt ctcacgtaat
     2281 tattcataaa gaggtgacga atgaataaat tatctaacgc acagctaaag gcttttgatg
     2341 aatggctgtt tgattatcgt tttattgata acaagattgc acttcgaaaa cttgagctac
     2401 aaactgatat gaatacagaa gtagataaaa atatcggtgg aggaagagca aattttgttt
     2461 caaaagttac tgaggatgtg gttgcaaggt gggattcaga tagggagcta aagggtctat
     2521 ccaatttcaa agaagcagtt attgcaacgc taaatctttt ggatgaagag ctaactgata
     2581 tttttaatct tagatggggt gttggttcaa gcaacacttg ggaagaaatt gcctatacaa
     2641 gacatctgtc gcgtaagtct atttaccgca aaagagagcg gattctagaa atattctcca
     2701 aaaaaatagg aatgtgacac aaaaatgtat ggaattgtca tctttttgat gataaattga
     2761 tagtatgagt ttaaaggtaa ggcaaatagt gtcttgcctt tttgttatgc ttggaggtga
     2821 tggaaaattg agtaaattaa ccctaaaaca gaagcgtttt gcagatgagt acatcatctc
     2881 agctaacgcg acagcagcgg ctattaaagc agggtatagt aaaaagacag caaggtcaat
     2941 aggtcaagaa aacttgacca aacctgacat taaagcttat atagacgagc ggcttgaaaa
     3001 acttgaatct gaaaagattg ctacgcaaga agaagtccta caatatctca cttcaattat
     3061 gcgaggagac caacaagaaa agacgctcat tagcgttgga gagtttggcc agaagatagt
     3121 tgacatcgat gtaggtgcaa aagatagaat caaggcagct gagctacttg gtaagcgata
     3181 tagattattc accgataaag ttgaaatgga tgtgtcatca gatataacta ttagcgtagg
     3241 tgagtgggat gacgattaaa cagcgcccta aaattaatat cgtaatcaag catcctagca
     3301 aagtttttaa caaacatatc tacgacaaac tttacaacta tagcaacttc acggaagttc
     3361 attatggtgg ggcgtctagc ggaaagtctc atggtgtttt tcaaaagata attttaaaag
     3421 cacttaaccc caaattcaaa catcccagaa agatattagt tctcagaaaa gttggtgcaa
     3481 cagttaggga ctctgtcttc gctgatatta tgtctaattt gtcgtatttt ggcgtcttgg
     3541 ataaatgcaa gataaatatg tcagctttta gaataacatt gccgaatggc tcggaattta
     3601 ttttcaaagg catggataac cctgagaaga ttaagtcaat taaggggata tctgatgtcg
     3661 tcatggaaga agctagtgag tttactcttg acgattacac acagcttacc ttacgtctta
     3721 gggacaagaa acatttagag aagcaagtct atctcatgtt caatcctgtg agtaaagcaa
     3781 actgggttta taaagctttc tttatcaagt cacctaagaa tacagttgtc tatcaaacaa
     3841 cttataaaga caaccgcttc ttagatgatg ttactagaga aaatatcgag gaactagccg
     3901 ataggaatga agcttattac aaaatatatg ctcttggcca gtttgctaca cttgataaac
     3961 taatttttcc caagtatgac aagcaaatat taaacaaaga caagttatca cacttgcctt
     4021 ctttttttgg tttggactat gggtttatca atgacccttc ggcatttttg catgttaaaa
     4081 tcgatgacgc aaacaagaag ttatacatct tagaggaata tgtcagaaaa aatttgacca
     4141 atgataaaat agcaaatgct ataaaggacc ttggctatgc caaagaagaa atcagaggag
     4201 attcggctga aaagaaatct aaccaagagc tgaggaattt aggtattcct agaatgattg
     4261 atgttaccaa aggtcctgga accgttatgc aaggaattca gtacctgctt cagtatgatt
     4321 ggattgttga tgaaaggtgt gtcaagacta ttgaagaact cgaaaattac acttggaaga
     4381 aagacaagaa gactaatgag tatatcaatg aaccagttga cagttacaac cactgcattg
     4441 acgccataag atatgccgta caagacagaa tatatcagtc ggcagataga agtaagcgca
     4501 tgaagaacgc gaagtactat ttttaggaat gaggtgagac tttggaagaa aaacaatttt
     4561 tagtaggaac tcgttttaat gagaatgcta ataagcaatt tattatgctg caagaagact
     4621 ttgaagcaat tgactatgaa tcaaaacttt ggatagatca acttaagaat tacatttcaa
     4681 gatttaaagc agaacaatta gagcgtttga aagagttaaa gcgatactat cttggggata
     4741 ataacatcaa gtatagacct gctaaaacag ataaatacgc agcggataat cgtattgcaa
     4801 gtgattttgc taagtatatt acggtatttg agcaaggtta tatgcttggc gttcccgttg
     4861 agtacaaaaa tgaaaataaa gacctccagg cagctattga ccttatgtcg gtcagaaaca
     4921 atgaggatta ccacaacgtc aaaataaaaa cagatttatc aatttatgga agagcctacg
     4981 agctgttaac tgttgaggaa atagatgaca aaaaaaccga agtaaaactc taccagctcc
     5041 cagccgagca aacttttgtt atctatgatg atacatacca gcgaaattcg ttgatggccg
     5101 ttcattttta cgacatagat tatggctcag gtaaacgcaa acagattatc aaagtatata
     5161 cttccgacac aatttatacc tacgaagact ataatttcga aacaaaaggt atgcgcttaa
     5221 aagagcatga agaacatttt tttaaaggtg taccagtcaa tgaatatgct aataatgagg
     5281 agcgtacagg ggcttatgag tctgtacttg ataatattga tgcctatgat ttatctcagt
     5341 cagagcttgc taattttcaa caagattcag tcaatgcgtt gcttgtaata gctggtaata
     5401 cttacacagg ggctgatgag aatgactact tagatgacgg acgattaaat cctaatggtc
     5461 gtttagcgat ctcaattggg tttaaaaaag cccaagtgtt aattttagac gataatccta
     5521 atccaaatgg cgttaaacca caagcgtact ttctcaaaaa agagtatgat accgccggta
     5581 gcgaagccta caaaaacaga ctagttgcag acattttgag atttactttt acgccagata
     5641 cacaagatat gaaattttca ggagttcaat ctggtgaatc aatgaagtat aagcttatgg
     5701 cttctgacaa ctatcgcgag aagcaagaga ggttgtttaa aaaaggctta atgcgacgct
     5761 tacgcttagc agctaatatc tgggccatca aagggaatga agcaactaca tatagccttg
     5821 taaatgatac gagtatagtt ttcacaccta atcttcctca aaacgataat gaaattgtta
     5881 cagctgcaca aaatctctat ggcattgtta gcgatcagac tatctttgaa atcttaaata
     5941 ctgtcacagg agtagacgct gaagcagagc ttgaacgttt gaaagaagaa gctgataaaa
     6001 agcaatcttt accggagcct agattggtag gtgatgctag tggtcaagaa gaaccaacag
     6061 cagaaaagcc ttaattactg gcaaaaacgt caagaagata ttctaagtta tttagacaga
     6121 actgatttag acgtcttttc agaactgcaa aagttatata atgaacaggc ttttgagtta
     6181 caaaaggaat tgtttgattt ttatactaag tattctgaag agaacaaaat gacttatcag
     6241 gatgtcgtta aaaagctacg tcatgaagat ttatcagatt acgtagctaa tgctaacaag
     6301 tatcgtaaac aagctgagaa agaccctgag ttgctaaaac gacttaatga gcagtatgta
     6361 tcagcaagag ctacaaggat ggatgcatta aatcttgaac ttgtttatcg cgcagggata
     6421 ctaaaaggtg tacttgattc agtatttgaa aatcatttaa aaaaagctgc ctcatatgct
     6481 tataaaaaag caatgggtgg acggtcaggg acaatcaatg gtccagtttt agaagaactg
     6541 gttagaaccc cgtttgatgg ttataactac tcagaacagt tgtggggcaa tactgacaat
     6601 cttgtcaaga atctccaaaa gaaattaaaa caaggttttg ttcgtggcga gcatcctaga
     6661 gcgatagcca gagatttagc taagcaattt aatgttgcta atcacagggc tgaaacacta
     6721 atcagaactg atggaactat ggtgattaat aatgctaccg ctaggcgcta cttgaatgca
     6781 ggacttaagt attatcgtga tttagttagg cttgatgaca ggacaactga gatatgtcgt
     6841 aaaatcgcta aagaaaacaa aagaaagcta ttatctgagc taaagcctgg aattaatgca
     6901 gcaccctatc atttcaactg cagaacaacc attattcctg atgaagatga gctaagcatt
     6961 gaagtagatc caattgatga taaaagcact aagtatttta aggatgtcac ctcagattgg
     7021 atagatggaa atgagcataa accacaactg tcactactaa atgagtatgt aaaaaatggc
     7081 actccttaca aagtagatgg ccataatgtt gttcttgatc actcgaatta tgaatataga
     7141 gttgctaatt ggttatctaa aaagacagga ttgcaagttg acatggtgcc aagagttaat
     7201 tcccctgaac atatcaatac tcctgattat ttagttgatg gagttccttt tgatctaaaa
     7261 gaaatcacag gttcgggtaa aaacgtcatt gatggcaatc tacgaaaagc aaaaaagcag
     7321 gcaactaata tcatttttga tataacaaaa acccctttat catttgaaga gataatgggg
     7381 cagttagaac acatttatat gttagatcgt aggggtcttg atatatcaat tatcaaaaat
     7441 aaagatgagg tattagctgt attagaaaaa gaaggacgat gacccaccgc ctccacagta
     7501 aactgcttca tgggcgttag accatcgtcc ttctttatct caattataca tcaacctatt
     7561 aaaactatca aggaggaaaa tatgtttatt tggcaaatgg tattagtggc actaggtgta
     7621 ttggtgctca tattgattgt tggcattgca gcaatagcgg ttaagtcaat tattgcagaa
     7681 ttgaaaaagg taggataaac atgaataagc gcattaagaa aaaacgtaaa ttggaaacag
     7741 cggttgtgat gcttgttgca gagaatgcca tgcaagcaga agctcttaga aatcaaaaca
     7801 aacaaattac agagctgaga gcgattgtac aacaaaacgc ccaagctacc aatgaagagt
     7861 ttgcgtctgt taaagctgct actttagata accaatcagc tattgcagat attgcaattg
     7921 acgttaacta tattaagaaa aattacaagc gaaaatgggg aaaataaatt ttaaacgaat
     7981 tcgacccctt tagaaatcaa agtcgtagcg atgcggcctt ttattatgtc caaactttgt
     8041 tgatgacact aaaagctaca ctgtttcgtc gcaggacgta aaactagatt atcggttggt
     8101 ggcgtaacca ctaggagaaa ttatggcaga agaaaatgta acaacagaaa ctactgaaca
     8161 agtcgacact cagcaagaaa ctgttgaaca tcctaagcag gaacatgagc gaacttttac
     8221 tcgcgcagat atctcaaaaa tgatgactgc tgaacgtgca aagtgggaag cagagcaatc
     8281 agaagcaatc gaaaaagctc gtactgaggg tgaaagacta gctaaattat caaaagatga
     8341 acgtgctaaa gaagaagaac aaaaacgtct cagtgctatt gcagaacgtg aaaaagcagt
     8401 tgcagaacgt gaaatgcgca tcgaaacaca atcattactt gctgaaaaag gattgccact
     8461 agattttatt gatgttgttt tagctactac tgcagatgaa gtgaaaacaa acattgataa
     8521 tttacagtct atctttgatc aagcagtgga aaatcgtgtt aacgaaagat taacgcaaaa
     8581 gtcacctcga actggtaatg gctctgtcgg tatgactaaa gctgaaatta tggctattga
     8641 gaatgatgat gaacgtctca aagctattgc agctaatcgt aatttattta caaggtaaga
     8701 aaggacatat taatttatgg cagaaaataa tttaaatgtg atgtcagact taggagaaat
     8761 caaatctatt gattttgtta ataagttttc aacgaatatc aatgaactac tcaagctttt
     8821 gggagtaact cgcaaacagg aattgactaa tgatctaaaa atccaaactt ataagtggtc
     8881 tactacttta gataagacaa atcccgccga gggtgaaaca attcctttat ctaaagttac
     8941 tcgtgcaaaa gctaaggaat atactgttga gtggttcaaa aaacgtcgtg caactaccgc
     9001 tgaagcaatt gcacgtcatg gtgtagctcg tgcgattaac gaagcagata cacgcattat
     9061 gcgagaaatt caaaatggtt ttaaaactaa cttctttgaa tttttaaaga agtcaactac
     9121 aaaagtgact ggaacaactc ttcaaagtgc attatcacaa tcatgggcaa aaattgctac
     9181 ttttaatgaa ttcgatggtt cacctatcat ctctttcgtt tctccacttg atgttgctaa
     9241 gtatctagga gatacaaatg taggtgcaga tgcttctaac gtgtttggaa tgacattgct
     9301 taaaaacttc ctaggtatgc aaaacgttat cgttttacca tcagtgccag agggaaaagt
     9361 atacacaaca gctgttgaaa accttgtgtt cgcttattta aacgtcaata gtggcgatct
     9421 tggagggatg tttgctgatt ttactgatga aactggattg attggtgcgg ctagaactcg
     9481 tcaactttct aatttgacct atgaatcagt attttttggt gcaaatgtgc tatttgcaga
     9541 aattccagaa ggcgttgtag aagcaacaat tgaggctcca aaagtagttc caggaggcta
     9601 atagatggat aaggagttat tactcacaga ggttaagcta ttcaaaggga tcagcgccaa
     9661 agacactagc caagatgatt tgataaaatt atccattaat gaaagtattg accgtatcct
     9721 tgcgaaactc aatgattact cagacacaga aatgactgaa attcctaagc gtcttacttt
     9781 tattgtcaga gatgtggcta ttaaaaggta taacagactt aattcagaag gaaccgttga
     9841 agatagcgaa gaaggaaaga cttttaagtg ggacagttac cttaaagagt atgaatcaac
     9901 actcagaagc gctgctattg ggaaggtata ttcaggcaaa ggggtagcaa gatttattta
     9961 ggaggtcata aatgatttac aaagatagag ttatcctagt gtatgtcgat gagcaggacg
    10021 attttctaga taaaagaact gttgaaaaac ctagcggaaa aatcccctgc atggaaaata
    10081 ctttcacaaa atctgaacag atggggttat ttggtaaata tgatttgaac gcttttaagt
    10141 tgcacttgca aggccgatac gatggcttta gcaagattat ctacaagggg aaaccgaggt
    10201 tgataaaggg actagcgcac cataaaaata gcacggttat ttatgtatga gtcttattta
    10261 tcggatgaga ggcctagata ggtttttacg cagcgttgag cgcaagcaga agtcagtacg
    10321 aatcgctgta gataaagagc ttagtaaatc agctgctagg attgagagac aggctaaaat
    10381 actagccccg gttgacactg gatggctgag agctcaaatc tacagtgagc aacaacgact
    10441 cttacactac agagtggttt ctcctgcttt atattctatt tatcttgagt taggtactcg
    10501 taaaatggaa gcccaaccat ttttagaccc tgccctgaga aaagaatggc cagtgttaat
    10561 ggctaatctc aaaaaaatgt ttaagaggtg atgcatggat tactcaccag aaacactata
    10621 tttaaaaaag gtaaaaaata gattgggagt tttagacata ccaatctatt ttaaattgcc
    10681 taaatcagac gttttagagc cttttattgt tgtaggtaca aatatatcag acttgtcaaa
    10741 aacagctcaa actggagcag ttattgatga ttttagcctg aatatcgatg ctttcttacc
    10801 tggcgatagt cgtttggatg cagaagagat aaaatctcgc atgcttagac tgcttgggcg
    10861 gaataaccaa ataaaggctc agattttggt agataattca ataggacgag aagtctatag
    10921 agttgctatc aacattacag aaatactaat ttaaaggaga attaaatgga aactacagtt
    10981 gaaattacta cagctaagcc tattaaaggt aaaaatatat tttattttat tcaatctgtt
    11041 gatgctgaat taggtagtaa agcaatactg ccagcgtatc gtacagatgg aacaacgaag
    11101 ctcggtggag attttatcga tgaacaaact caacaaggac gtgtgcttga aaaagcaaca
    11161 gatgagcact caattgattt gacatcatac tacacgccga aagacaaagc aattgaaatt
    11221 gtagaaatag ctaaaacaac tggtaaatct gttaaagttt ggcgtgttat cgcagacgaa
    11281 tctttgaaag aaaaaggttc aggaacttta gagggtaaag aagtttatcc cgcgaagttt
    11341 ggatatggaa aaattgacga gattgaatat tcagaaggtg ttgaaaaact tgtagaagca
    11401 tcatatacat tgaatattat cgatagttta aaagatggca aatttccact gacggcagaa
    11461 gatatcgctt tgttaacaag tgtttatgca tatcaaaacc caggcgaaac tactggcgat
    11521 tatgacaata tccagaaaaa agaagtttca ggaagtttac tgggagcagt tccagggata
    11581 tcatcagaag gacactaatt tataaggtag catttcgc
//
LOCUS       DATROD010000052        11599 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.052, whole genome shotgun sequence.
ACCESSION   DATROD010000052 DATROD010000000
VERSION     DATROD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11599
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.052"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            155..859
                     /locus_tag="VBC74_001269"
     CDS             155..859
                     /locus_tag="VBC74_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942216.1"
                     /translation="MRLVWKFLSEKLVCKIMDSSYGVLILFFICLSITAVGGYTLVYN
                     LSGYSSMDAQKVFGIIVLTIILLVFIVGTIFYAIGSAQLIMSILSKKKISFDEKDAPL
                     DGATHVGETVFQGKEVSLYSKQDKFFLWSRKWQKGFSKYQYYHIRPVDQTEISEVYQV
                     ERCCFYCDHIFTQFFIREGKNREKIVELCTIDVQIGNDRDLGFKQVNRGEFEKTVPFK
                     DVEIKEIKKLIDLKQI"
     gene            1285..2001
                     /locus_tag="VBC74_001270"
     CDS             1285..2001
                     /locus_tag="VBC74_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6942217.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKQGEPDPESN
                     SALKFVLDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADTVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            2156..2845
                     /locus_tag="VBC74_001271"
     CDS             2156..2845
                     /locus_tag="VBC74_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEO6942218.1"
                     /translation="MLTKPIIGITGNEREMSDIPGYYYDSVSRHISEGVKNAGGLPVI
                     LPISEAESAKAYVEMIDKLIISGGQNVLPSYYGEEKIIESDDYSLARDIFEFALVEEA
                     LKQNKPIFAICRGMQLVNVALGGTLNQSIDNHYQEPYIGFAHYLNVEKGSFLEGFISG
                     DFKINSLHRQSVKLLAEGLIVSARDPRDGTVEAYESRTEQCIIGVQWHPELMLHQIEN
                     QTLFGYFVNET"
     gene            2947..3780
                     /locus_tag="VBC74_001272"
     CDS             2947..3780
                     /locus_tag="VBC74_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6942219.1"
                     /translation="MTIKKVLSVTGIILVTVASLAACSSKSHTTKTGKKEVNFATVGT
                     TAPFSYVKDGKLTGFDIEVAKAVFKGSDNYKVTFKKTEWSSVFTGIDSGKFQMGGNNI
                     SYSSERSQKYLFSYPIGSTPSVLAVPKNSNIKAYNDISGHKTQVVQGTTTAKQLENFN
                     KEHQKNPVTLKYTNENITQILTNLSDGKADFKLFDGPTVNAIIKNQGLTNLKTIPLTM
                     RDQPYIYFIFGQDQKDLQKYVNNRLKQLRKDGTLSKIAKEYLGGDYVPNEKDLVTPKE
                     K"
     gene            3954..6191
                     /gene="metE"
                     /locus_tag="VBC74_001273"
     CDS             3954..6191
                     /gene="metE"
                     /locus_tag="VBC74_001273"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEO6942220.1"
                     /translation="MVKVSNLGYPRLGEQREWKQAIEAFWAGNLEQKDLEKQLKQLRI
                     NHLKKQKEAGIDLIPVGDFSCYDHVLDLSFQFNVIPKRFDEYERNLDLYFAIARGDKD
                     NVASSMKKWFNTNYHYIVPEWEVETKPHLQNNYLLDLYLEAREVVGDKAKPVITGPIT
                     YVSLSSGIVDFEATVQRLLPLYKQVFQDLIDAGATYIQIDEPIFVTDEGELLVDIAKS
                     VYDFFAREVPQAHFIFQTYFESAVCLDKLSKLPVTGFGLDFIHGRAENVAAVKQGLFR
                     EKELFAGIVNGRNIWAVNLEETLALLEEIGPFVKRLTLQPSSSLLHVPVTTKYETHLD
                     PVLKNGLSFADEKLKELELLASAFDGNKTKGYHEALSRFSALQAADFRHVALESLAEV
                     RLERSPYKLRQALQAEKLQLPILPTTTIGSFPQSPEIRKKRLAWKRENLSDSDYKDFI
                     KTEIRRWIAIQEDLDLDVLVHGEFERVDMVEFFGQKLAGFTTTKLGWVQSYGSRAVKP
                     PIIYGDIKHIQPLSLEETVYAQSLTKKPVKGMLTGPITITNWSFERDDISRSDLFNQI
                     ALAIKDEIQLLEQSGIAIIQVDEAALREGLPLRQQKQQAYLDDAVAAFKIATSSVKDE
                     TQIHTHMCYSKFDEIIDSIRSLDADVISIETSRSHGDIIESFETAVYPLGIGLGVYDI
                     HSPRIPTKEEIIVNIQRSLKCLSKEQFWVNPDCGLKTRREAETIAALEVLVSATKEVR
                     QQLDN"
     gene            6236..8080
                     /locus_tag="VBC74_001274"
     CDS             6236..8080
                     /locus_tag="VBC74_001274"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEO6942221.1"
                     /translation="MSKFLEKLKTDILVADGAMGTLLYTYGLDTCHESYNVTHPEKVL
                     AIHQAYIEAGADVIQTNTYGAQRHRLKNYGLEDQVVSINQAAVNIAHQATLGKETFIL
                     GTVGGFRSQRQCDLTLDNIVEETLEQVEALLVTGQLDGLLFETYYDIEEITTVLKIVR
                     EMTDLPIITNISLHEAGVTSNGKPIVEALSQLVMLGADVIGLNCHLGPYHMIQSLKQV
                     PLFAQSYLSVYPNAGQLSLDGENSQYQFSQNSEYFGKSAELLVAEGVRLIGGCCGTTP
                     DHIRAVKRSIRGLKPIERKVVTPIIPVKDFVRRIRRTDTLVDKVKKEVTIIAELDPPK
                     HLDIVQFQKAIRAIDQKGIAAITLADNSLSNTRICNLSIASLLKDEISTPFLLHIACR
                     DHNLIGLQSRLLGMELLGFNHILAITGDPTKLGDFPGATSVYDVTSFKLLSLIKQLNQ
                     GLSYSGASLRRPTDFTVAAAFNPNVKNLTRTVKLIEKKVASGADYFMTQPIFDHSVLR
                     ELADLTKTVEQPFFIGIMPITSYNNAVFLHNEVPGIKLSESFLSALEKVKDDKEACLT
                     LALNESKSLIDEALNHFNGIYLITPFLRYDLTLELIDYIQKKQVRKSS"
     gene            8427..9134
                     /locus_tag="VBC74_001275"
     CDS             8427..9134
                     /locus_tag="VBC74_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEO6942222.1"
                     /translation="MVCVDFIDQKVYSEAEGLFVSDLDLGIKGLIRARYPFALDSDFI
                     SYGNLSHYCMNYLDEIVQRANQKNEKIKYNVTTLMKEEEKPFNVEERLNKQATIGQRI
                     ADDVARFGGSWTFIIVFVSIMAIWMLVNIMKPFGIQFDPYPFILLNLALSTIAAIQAP
                     LIMMSQNRAADYDRLQARNDFNVNKTSELEIRLLHEKIDHMVQQDQFELLEIQKLQTE
                     MLVSLGNQLAQLKQLQK"
     gene            complement(9299..10384)
                     /locus_tag="VBC74_001276"
     CDS             complement(9299..10384)
                     /locus_tag="VBC74_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO6942223.1"
                     /translation="MTTNVMANYSYGEDCFKEIPSVLATYRIQSIAFVGGKRALDSSE
                     AEVRSILEEANIKVTGSFVYGTDSTQSNIDKLVANPQVQAADAILGFGGGKALDTAKM
                     VAKELGKNSFTIPTICSNCSAGTAIAVVYNDDHSFLRYGYPESPLHIFINTRIIAQAP
                     SKYFWAGIGDGISKAPEVERATLEAKTNKLPHTAVLGQAVALSSKEAFYQFGEQGLKD
                     VEANLASRAVEEIALDILISTGYASNLVNQPDFYYNSCHAHAFYYGTTAIQRQGEFLH
                     GVVVAFGVLVLHAYFNELEELEKVARFNKSLGLPTTLADVSLSEKDIPKIVEIAMTTN
                     EYKNTPFDPKMFAQAILAADAFGQTLA"
     gene            complement(10585..11124)
                     /locus_tag="VBC74_001277"
     CDS             complement(10585..11124)
                     /locus_tag="VBC74_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEO6942224.1"
                     /translation="MKIAIIGYSGSGKSTLARKLGNYYNCNVLHLDSIHFAPNWEERK
                     YDDMIDDVSSMLEKRTWIIEGNYKKLLYQERLADADEIIFFDFNRFNCLWRAFKRYCK
                     FRGKTRPDMANGCPEKLDFEFISWILKDGRSDKQKSNYKQVVEDYPQKIKVLKHQRDL
                     DQYLKELPVASNTDPLKSL"
BASE COUNT         3846 a         1785 c         2253 g         3715 t
ORIGIN      
        1 aaaataactc agcttacttc aattatagcc atgtagcata gaattttatt gagtttttga
       61 tttttgatga catgagacta gatgatcaat atccgaatat ggtcacttta atgtgtatag
      121 ctattatatt aatatattta gggagggaag tgttgtgcgc cttgtatgga aatttctatc
      181 agaaaagtta gtatgtaaga tcatggattc ttcgtacggt gtgttgatat tattttttat
      241 ttgcctatca ataactgctg taggtggata tacattggta tataatttgt ctgggtattc
      301 ctcaatggat gcacagaaag tttttggaat cattgtacta acaataattt tactagtatt
      361 tattgtagga acaatttttt atgcaatagg ttctgcacag ttgataatgt cgattttgtc
      421 taagaaaaag atttcgtttg atgagaaaga tgcaccactt gatggtgcga ctcatgtggg
      481 agagacagtt ttccaaggaa aggaggtgtc gttatatagt aagcaggata aatttttcct
      541 atggagtaga aagtggcaaa agggtttcag caaatatcaa tattatcaca tacgaccggt
      601 tgatcaaact gaaattagtg aggtgtatca agtggaaaga tgttgcttct attgtgacca
      661 tatatttaca caatttttta ttagagaagg aaaaaataga gagaaaatag ttgagttatg
      721 cactattgat gtacaaattg gtaatgacag agatctagga tttaaacaag ttaatagagg
      781 cgagtttgaa aaaacggttc cttttaagga tgtcgaaatt aaagaaataa agaaattgat
      841 tgatttgaaa caaatataag aaaagcacct ttaaaagggc ttttcttata tttttcttta
      901 aaaagctcgt tattcgtttt gtagtgtgct acaattctat tgaattaatc gaataaaaga
      961 agcgcagtta tggtaaccac tacgctttac cgagtaagtc caattacaac gttcccttac
     1021 ttagtcttta tcgtaagata caggataaca ccgaatctgt caaggctggg caaaggaaaa
     1081 tttgttcagt ctttttataa aaggaactcg gtaggttctc cttaatagaa aattgacaat
     1141 tctaaaaaat tctactgcat accgtgatag aatttccatt ttaccaattg ctatcaaagt
     1201 gctataataa tagagttaaa attaagattg cttgccaata tgagccagca ggttattaaa
     1261 aatgaaatca aaggagattt tccaatggga cgtaagtggg ccaatattgt tgccaaaaag
     1321 actgctaaag atggtgctaa ctcaaaagta tacgctaaat tcggtgttga aatatatgtt
     1381 gctgcaaagc aaggtgaacc agaccccgag tcaaactcag ctctaaaatt cgttttggac
     1441 cgtgctaagc aagcacaagt tccaaagcat gttattgata aagcgattga taaagccaaa
     1501 ggaaacacag atgaaacttt cgtagaggga cgctatgaag gttttggtcc aaatggttca
     1561 atgattattg tggatacttt gacatcaaat gttaaccgta cggcagcaaa tgtacgtact
     1621 gcttacggta agaacggtgg caatatggga gcttcaggat cggtatccta cttatttgat
     1681 aaaaaaggtg tcatcgtttt tgctggtgat gatgctgaca ctgtcttcga acaattactt
     1741 gaagcggatg tagacgtaga tgatgttgaa gcagaagagg gaacaataac agtttatacc
     1801 gccccaacag atcttcataa aggtatccaa gcacttcgcg ataatggtgt agaagaattc
     1861 caagttactg aacttgaaat gattcctcaa tcagaagtag tattggaagg tgatgacctt
     1921 gaaacttttg aaaagcttat tgacgcactc gaatcagatg atgatgttca aaaagtttat
     1981 cataatgtag cagatttcta aaaaataaaa ccgtagtatt aatactatgg tttttatttt
     2041 tgtgagagtt gataggtatt gttaaagaag tgataggtaa aaaatattat tcaaaaagtt
     2101 tttttaggtg taaaatgata attattagac agttattggt tgatgaggga gcaaaatgtt
     2161 aacaaagcct attattggta ttacaggaaa tgaaagagaa atgtcagata ttccaggtta
     2221 ttattatgat tcggtatcta ggcatatttc ggaaggagtt aaaaacgcag gcgggttacc
     2281 agttatccta ccaataagtg aagcagaaag tgccaaagct tatgttgaga tgatagataa
     2341 gctgatcata tctggtggcc aaaatgtttt accatcctat tatggtgaag agaagataat
     2401 tgagagtgat gattattcac ttgcgcgtga tatttttgaa tttgcattag tcgaagaagc
     2461 gttgaaacaa aataaaccaa tctttgcgat ttgccgtggc atgcagttag taaatgtggc
     2521 gcttggtggt acacttaatc aatctattga taatcactat caagagcctt acattggatt
     2581 tgctcattat ttaaatgtgg aaaaagggag ttttctagaa ggctttatat caggtgattt
     2641 taagatcaat tctcttcata gacaaagtgt taaactgtta gctgaaggtc tgatagttag
     2701 tgctagagat cctagagatg ggacagtaga agcttatgaa agtcgaacag aacaatgtat
     2761 tataggtgtt caatggcatc cagaattaat gcttcaccaa attgaaaacc aaaccttatt
     2821 tggttatttt gttaatgaaa cgtagcatcc atataatagc aaaaactctc agacgataga
     2881 agataagtat tagttaaaaa cttaaagatg ttatgatgtc gtgtacaatt aaagaggaga
     2941 aaaaagatga caataaaaaa agtgttaagt gtaacaggaa ttattttagt gacagtagcg
     3001 tctctagctg cttgtagctc aaaatctcat actactaaga cgggcaaaaa agaagttaat
     3061 tttgcaactg ttggaacaac ggcacctttt tcttatgtga aggatgggaa actgactggc
     3121 tttgatattg aagtagccaa agctgttttt aaaggttcag ataactataa agtcactttt
     3181 aaaaaaacag aatggtcatc ggtatttacc ggcattgatt caggaaagtt tcaaatgggt
     3241 ggaaataata tttcttattc atcagagaga tctcaaaaat atttattttc atacccaata
     3301 ggctctactc cttcagtttt agcagttcct aagaatagta atatcaaagc ttataatgat
     3361 attagtggtc ataaaacaca ggttgtccaa ggaacgacaa ctgccaagca attagaaaat
     3421 ttcaataaag agcatcagaa aaatcctgtt actctaaaat atactaatga aaatattaca
     3481 cagattctaa cgaatttgag tgatggaaaa gctgatttta aactttttga cggaccaact
     3541 gttaacgcta tcataaagaa tcaaggtctc acgaatttaa aaaccatccc tttgactatg
     3601 agagatcagc cttatattta tttcatcttt ggtcaggatc aaaaagactt gcaaaagtat
     3661 gtgaataatc gccttaaaca attacgtaaa gatgggacac tttcaaaaat agctaaagaa
     3721 taccttggag gtgattacgt accaaatgag aaagatctcg ttactcctaa ggagaagtag
     3781 taataaaaac taaggagatg tcaatcctta gttttttgtt atatagttta aaactatata
     3841 aattgctaaa aatataaaat attaaaagct attgatatta tagttttcat ggactaaaat
     3901 aattcataaa actaaaaggg ttagttatta gctataactt gatggaggaa attatggtaa
     3961 aagtttcaaa tttagggtat ccacgtcttg gtgaacagcg cgaatggaag caagcgatcg
     4021 aagctttctg ggcagggaat cttgaacaaa aagatttaga aaaacaacta aaacaattac
     4081 gtatcaatca tttaaagaaa caaaaagagg caggtattga ccttattcca gtgggggatt
     4141 tttcttgtta tgatcatgtt ttggatttgt catttcaatt caatgtaatc ccaaagcgtt
     4201 tcgatgagta tgagaggaat ttagaccttt attttgctat tgcaagaggt gacaaagata
     4261 atgtcgcatc atctatgaaa aagtggttta ataccaacta ccactacata gtcccagaat
     4321 gggaggttga gactaaacct cacttgcaga ataattactt acttgatctt tatctagaag
     4381 ctagggaagt agttggtgat aaagcaaagc cggttatcac tggtccaata acctatgttt
     4441 ccttatcatc aggaattgtc gactttgaag cgactgttca gcggttatta ccactttata
     4501 agcaggtctt tcaagatctg atagatgcag gcgccaccta tattcagatt gatgagccga
     4561 tatttgtaac tgatgaaggt gaacttttag tagatatagc taagtctgtc tatgattttt
     4621 ttgcaagaga agtaccacaa gcccacttca tctttcaaac ctactttgaa tcagcagtct
     4681 gtttagataa actctctaag ctgccagtaa cgggatttgg ccttgatttt atacatggta
     4741 gggcggaaaa tgtagctgct gttaagcaag gtctattccg cgaaaaagaa ttatttgcag
     4801 gaattgttaa tggtcgaaat atctgggcag taaatttaga agaaacgttg gctttattgg
     4861 aagagatagg tccctttgtt aaacgtttga cccttcaacc ttcttcaagt cttttacatg
     4921 taccggtgac gactaaatac gaaacacatt tagaccctgt gttaaagaat ggcttatcat
     4981 ttgctgatga aaaactaaag gaattagaac tattagcgag tgcttttgat ggtaataaaa
     5041 caaagggata tcacgaagct ttatctcgtt tttcagctct tcaagctgct gattttcgtc
     5101 atgtagcgtt ggaatcatta gcagaagtaa ggcttgaacg aagtccgtat aaattacgcc
     5161 aagctttgca agctgaaaaa ttacagttac cgattttacc aacaacaact attgggtcct
     5221 ttcctcaatc acctgaaatt aggaagaaac gccttgcttg gaaaagagaa aatctatctg
     5281 actcagatta taaagatttc ataaaaactg aaattagaag atggattgct attcaagaag
     5341 atcttgatct tgatgtgtta gtacatggcg aatttgagcg cgtggatatg gttgaatttt
     5401 ttggtcaaaa gttagctggt tttacgacaa ccaaattagg ctgggtacag tcttatggtt
     5461 caagggcggt caaaccacct atcatttatg gtgatatcaa acatattcaa cccttaagcc
     5521 ttgaagaaac ggtttatgcc caaagcttga ctaagaaacc tgttaaaggc atgttgacag
     5581 gtcctattac tataacgaac tggtcatttg agcgagatga tatcagccga tctgatcttt
     5641 ttaatcaaat tgctttggct ataaaagatg agattcaact tttggaacaa tcaggtattg
     5701 ctattataca agtggatgaa gcagcccttc gagaaggctt acccttacgc cagcaaaagc
     5761 aacaggctta cttagatgat gctgttgcgg cctttaaaat tgcaacttca tctgtgaaag
     5821 atgagacaca aattcataca catatgtgtt attcaaaatt tgacgaaatt attgattcta
     5881 tccgttcact agatgcagat gttatttcta ttgaaacgag tagaagtcat ggggacatca
     5941 ttgaaagttt tgaaacagca gtttatcctc taggaattgg tctgggtgtt tatgatattc
     6001 attctcctcg catacctact aaggaagaaa ttattgtcaa tattcaacga tcactaaaat
     6061 gtctatcaaa agagcaattt tgggtaaacc ctgactgtgg cttaaaaaca cgtcgtgaag
     6121 cagaaacaat tgctgccttg gaggttcttg tttcagctac caaagaggtt cgtcagcaat
     6181 tagataatta ataggttaat cagtaaagga ttgccatgag ggaggagtaa agaagatgtc
     6241 caagttttta gagaaattaa aaacagatat tctagtagct gacggtgcaa tgggaactct
     6301 cctttatact tatgggctag atacttgtca tgaatcctac aatgtaacac atccagaaaa
     6361 ggttctagcc attcatcaag cttatattga agcgggtgca gatgttattc agacgaatac
     6421 ctatggggct caacgtcatc gattaaagaa ctacggtttg gaagatcagg ttgtctctat
     6481 caatcaagca gcagttaata tagctcatca agcgactttg ggtaaagaaa catttatttt
     6541 aggtactgtt ggaggttttc gttctcaaag acaatgcgat ttaacattgg ataatattgt
     6601 agaagaaact ttggaacaag ttgaggcttt attggtgaca ggacagctag acggattatt
     6661 gtttgaaact tattatgata ttgaggagat aacgacagtt ttaaaaattg tgagggagat
     6721 gactgatcta ccaattatta ctaatatttc tcttcatgaa gcaggggtaa catcaaatgg
     6781 aaagcctatt gtagaggcgc tcagccaatt agttatgcta ggtgcagatg tgattggctt
     6841 aaattgtcac cttggaccct atcatatgat ccaatcattg aagcaagttc ctttatttgc
     6901 ccagtcttat ttgtcagttt atcctaatgc aggtcagtta tctttagatg gtgagaatag
     6961 tcaataccag tttagtcaga attcagagta ttttggaaaa tctgcagaac ttctcgtagc
     7021 agaaggagtt aggcttattg gtggttgttg tgggacaact ccagatcata ttagagctgt
     7081 gaaacgatct attagaggat tgaaacctat tgaacgaaag gttgttactc ctataatacc
     7141 tgtaaaagat tttgttaggc gtatcagacg aacagatact ctagtggata aggtaaaaaa
     7201 agaagtaaca attatcgcag aattagatcc tccaaaacat ttagatatcg ttcagtttca
     7261 aaaggctata cgagcgattg accaaaaagg tattgcagca attactttag cagataattc
     7321 actatctaat acacgtattt gtaatttaag tatagctagc ttgcttaaag acgaaattag
     7381 tacacccttt ttactacata tagcttgtcg tgaccataat ttaattggtt tacagtctag
     7441 actgttgggc atggaattat taggttttaa ccatatttta gccataacag gagatccgac
     7501 taaacttggt gatttcccag gagcgactag tgtttatgat gtgacttcct ttaaactcct
     7561 ttctctgatt aaacaactta atcaaggttt atcttatagt ggtgctagct tgagacgtcc
     7621 aacagatttt acagttgctg ctgcctttaa tcctaatgtg aaaaacctta ctcgaaccgt
     7681 aaaattgatt gagaaaaaag tagcttctgg tgctgattat tttatgacac aaccaatatt
     7741 tgatcactct gtgttaaggg aattagctga tcttactaaa acagttgaac aacccttttt
     7801 tatcggaatt atgccaataa caagttataa taacgctgtt tttctacaca atgaagttcc
     7861 tggtattaaa ctttctgagt catttttatc tgctcttgaa aaggtaaagg acgacaaaga
     7921 agcttgttta actttggcct taaacgagag taagtccttg attgatgaag ccttgaacca
     7981 ttttaatggt atctatttga ttactccctt tttacgctat gatctgactc ttgagttaat
     8041 tgactatata caaaaaaagc aagtaagaaa gtcctcttaa ctttcttact ttttagttgg
     8101 tcgtttatta ttataataaa agttacgtac caaaacagag gttaaactag atttttaagc
     8161 cactttctat actaagataa attagttaat ttaagttttc tttaagttta tagacttaga
     8221 ataaagcgtt attttgatgg gtagcataaa atcaaggcat aatatagatg tcagcgattt
     8281 ttttagacta acgaaataac gatgtaaagc ttaatagggg tgtatcgaat actcacttat
     8341 atagtaccaa aagcctatag cctgttggct agcttgaccg tattcaaatg ttgaataaat
     8401 cttggttgag aaagggaggt ataaaaatgg tatgtgtcga ttttattgat caaaaagttt
     8461 atagtgaagc agaaggtcta tttgtttccg atttagactt aggcattaaa ggtttaataa
     8521 gagcacgtta tcctttcgct ctggatagtg actttatttc gtatgggaat ttaagtcatt
     8581 attgtatgaa ttatcttgat gaaattgttc agcgagcgaa tcagaaaaat gagaagataa
     8641 aatataatgt gactaccttg atgaaagaag aagagaagcc cttcaacgtt gaagagcgtt
     8701 tgaacaagca agcaactatc ggtcaacgaa ttgctgatga tgttgcacgt tttggcggat
     8761 cttggacttt tattatcgtt tttgtatcca ttatggctat ttggatgttg gttaatatca
     8821 tgaagccttt tggtattcaa tttgatcctt acccatttat cctgttaaac ttagctttat
     8881 caacgattgc tgcgatccaa gcaccactta ttatgatgag tcaaaatcgt gctgctgatt
     8941 atgatcgttt gcaagctcga aatgacttta atgtcaataa aacctctgag ttagaaatta
     9001 gattattgca cgaaaaaatt gaccatatgg tacaacaaga tcagtttgaa ttgttagaga
     9061 ttcaaaagtt acagacagaa atgctagtat cattgggaaa tcagttagca caattgaaac
     9121 aactacaaaa ataatctttt agcagttatt taaaaatact gatacaatac taagaattta
     9181 aattaatact gcaacctcct ataatggaga gcagtatttt tactttatta ataacaaaaa
     9241 ccccttagat gatgtgtcta agggattttt gtatcggaat aatcgagttg gtgaattatt
     9301 aggctagtgt ctgtccgaaa gcgtctgctg ctaaaatagc ttgagcaaac attttgggat
     9361 cgaaaggggt atttttgtat tcattagtag tcattgcaat ttcaacaatt tttggaatat
     9421 ctttttctga taaagagacg tcagctagag ttgttggcaa acctagtgac ttattaaaac
     9481 gagctacttt ttcaagttct tctagttcgt tgaaataagc atgtaaaact aacacaccaa
     9541 acgcaactac aacgccatgt aagaattcac cttgtctttg aatagcggtt gtaccatagt
     9601 aaaatgcatg cgcgtgacaa gaattgtagt agaaatcagg ttgattaact aaattagaag
     9661 catagccagt tgagattaag atatcaagcg caatttcttc aactgcacgc gaagctaaat
     9721 tagcttcaac gtcttttaga ccttgttcac caaattgata aaaagcttcc tttgaagaca
     9781 gagcgactgc ttgtcctaac actgcagtat gtggtagttt attggtctta gcctctaagg
     9841 tagcacgttc tacttcaggg gcttttgaaa taccgtcccc aataccagcc caaaaatatt
     9901 tgcttggtgc ctgtgcaatg atccgtgtat tgataaaaat atgaagtgga gactcgggat
     9961 agccgtatct taagaaagaa tggtcatcat tatatacaac tgcaatggca gtacctgcgg
    10021 agcaattaga acaaatagtt gggatagtaa agctattttt acctaactct tttgctacca
    10081 ttttagctgt gtctaaagct tttccaccgc caaaacctaa gatagcatca gcagcttgaa
    10141 cttgtgggtt ggctactaac ttgtcaatgt tagattgtgt tgaatccgtt ccatagacaa
    10201 agcttcctgt gactttaata tttgcttcct ctaaaataga gcgaacctcg gcttctgagc
    10261 tgtctagggc acgtttgcca cccacgaaag caatgctttg aatacggtaa gttgccaaaa
    10321 cgctaggaat ttctttgaag caatcttctc cgtagctata atttgccatt acatttgttg
    10381 tcattcagtg tcctcctcta ttccaaaggt cgattagaga cagctatttt ctaaaagtga
    10441 atagtttttt gtctcttgac caagtgatta tcatattata gcagaatatt cagaaaattg
    10501 gaacatgaaa aataatttaa ttttcaagaa taagaataga aaaggtaaag cgagatatac
    10561 gagtaaatac tgtcagtata ataattataa gctcttgaga gggtctgtat tggatgcaac
    10621 agggagttct tttaggtatt gatcaagatc gcgttggtgt tttagcacct ttattttttg
    10681 agggtagtcc tcaacaactt gtttatagtt tgatttttgt ttgtcagagc gaccgtcttt
    10741 aagaatccaa ctgataaatt caaaatctaa tttttccggg catccattgg ccatatcagg
    10801 tctagtctta ccacgaaact tacagtaacg tttgaatgct ctccacaagc agttgaagcg
    10861 attaaagtca aaaaaaataa tctcatctgc atctgctaga cgttcttgat atagcagttt
    10921 tttgtagtta ccttctataa tccatgttct tttttctaac atgctgctga catcgtcaat
    10981 catgtcatca tattttctct cttcccaatt tggggcgaag tgaatactat caaggtgtaa
    11041 tacattgcaa ttatagtaat ttcccaattt tctcgctaaa gtggacttac ctgacccact
    11101 atatccgatt attgctattt tcatatacta cctccgttaa attgtttact tgaagttaat
    11161 gctgtactta ggatttactt attttataag ggttattcac ttttttagat taatatattt
    11221 ttgatataag ttatccatac acatggtaac ataattacca gtaaatgtaa tctagatatc
    11281 aatactatgg ttgacgatat tgttaacttt tgggttcaaa aaattggggt aaaaatcatc
    11341 ggagggaact gccaaacaaa ctcacagttt aaaatactaa gtaataaaaa gtcttgaatt
    11401 tattatgttt aaggcttttt aagcgtctaa aatacttagt ctagtatgaa tttctaaaca
    11461 gaaagtaaaa ataatgagtt ttagtatggt ttttgggcat aatggaacga ttttatctaa
    11521 aatagtacgt ttcttattaa atttattttt ttagaaaaac attaggctaa cctatttgat
    11581 aaaaatagtg ataagtaga
//
LOCUS       DATROD010000053        11532 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.053, whole genome shotgun sequence.
ACCESSION   DATROD010000053 DATROD010000000
VERSION     DATROD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11532
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.053"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..1051
                     /locus_tag="VBC74_001278"
     CDS             <1..1051
                     /locus_tag="VBC74_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEO6942225.1"
                     /translation="FIKLGIATLLTVISLYTPINLATNHTTENIVTAQEYKTKENGTL
                     PFKHKRQLVLGELDDKGRATFAHIQLKVKDEPKKKRVKRLKTTPVGWHNFKFYYNDGT
                     QKAWLMSRGRLICHQFSGLNNERKNLVPMTNWLNTGNYNSTNSSNPESMLFYEKQLKT
                     WLSTHKNYYLDYKVTPIYQNNELIPRKIELKYVGIDKTGKLLPIFIGNKSTQDQFGIS
                     TVTLENTSPNATIDYLSGKAQNTVLSAKEQRKLIAKHEEEKRLAEKKVEEEKAAAETQ
                     KKLEEEQARLAAEAQRKQKEEQARLAAETQKKQETLVQEQTSQGYKRDYRGRWHRPNG
                     QYASKAEIAAAGLQW"
     gene            complement(1159..2466)
                     /gene="eno"
                     /locus_tag="VBC74_001279"
     CDS             complement(1159..2466)
                     /gene="eno"
                     /locus_tag="VBC74_001279"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEO6942226.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNVIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEALRWGAEVFHALKKILKERGLETAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDAERKVYDYSKFEGEGGAVRTAAEQI
                     DYLEELVNKYPIITIEDGMDENDWDGWKALTERLGGRVQLVGDDFFVTNTDYLARGIK
                     EEAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            2664..3119
                     /locus_tag="VBC74_001280"
     CDS             2664..3119
                     /locus_tag="VBC74_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEO6942227.1"
                     /translation="MTELDNKILQKASGENRLDPDQQRYYLGTYKERVVLHLSLKEAQ
                     EEKFQLAITSALNNLILEYPNLSLKLSPNLPTDVQMFYMKLANRLSLAATIVNEANAN
                     SPFGIIIHSDKAENVEWNDFETQFPDLFNSPKKEESPKKSLWQHFFSQK"
     gene            3132..3617
                     /locus_tag="VBC74_001281"
     CDS             3132..3617
                     /locus_tag="VBC74_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEO6942228.1"
                     /translation="MEIWDAYDQYGFITGLTLNRDQNIPQGLFHLVVDVILFHEDGDV
                     LMMKRHPKKKAFPAYFEATAGGSALKGENAKQAILRELKEETGIVPQCLTFLNREWFS
                     ERSYFVDHFIAKYNGAKDIITLQEGETVDYIWLKPEYIDLFLSKNKLIPSQIKLLKSL
                     I"
     gene            complement(3638..4279)
                     /gene="serB"
                     /locus_tag="VBC74_001282"
     CDS             complement(3638..4279)
                     /gene="serB"
                     /locus_tag="VBC74_001282"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEO6942229.1"
                     /translation="MIKGLLVMDVDSTLIMEEAIDLLAIEAGVGKQVAALTDAAMRGE
                     LDFEEALKKRVALLKGLPVTILTDILSSIHFTPGAYELIKECHKRQMKVGLVSGGFHE
                     TIDILAKQLQVDYVKANRLGVKGGFLTGEVEGEIVTKEVKKIKLKEWASENHLDLSQT
                     IAMGDGANDLPMIKSAGVGIAFCAKPIVREEAAYQINEKNLLKVFDIIDANEN"
     gene            complement(4373..6097)
                     /gene="ezrA"
                     /locus_tag="VBC74_001283"
     CDS             complement(4373..6097)
                     /gene="ezrA"
                     /locus_tag="VBC74_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEO6942230.1"
                     /translation="MSSGIILLLVAIVLLVIIAYVVGVVIRKRNDTLIANLETRKQEL
                     VDLPVQEEIEQVKLLHLIGQSQSTFREWNQKWTDLSTNSFKDIDFHLVEAENLNDSFN
                     FVRAKHEIDNVDSQLTIIEEDIVSIREALEVLKEQEEKNSARVTHALDLYETLQKSIS
                     EKEDNYGTTMPEIEKQLKNIEAEFSHFVTLNSTGDPIEASEVLNKAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKDIEQRFQEVREAIRSNSDGLV
                     SLDLDRARDENEHIQEKIDKLYDIFEREIAAYKVAHKDSKIIPQFLAHAKSNNEQLGH
                     EIKRLSAKYILNENESLSLRSFTNDLEEIETKVLPSVENFGQEASPYTHLQILFERTL
                     KTLTTVEENQMEVFEAVKTIESVETRARQNMDKYVNKLHMIKRFMEKRNLPGIPQDFL
                     STFFTTSSQIEALINELSRGRIDIEAVSRLNDVTTNAIANLEQATYLVVQDATLTEQL
                     LQYSNRYRSFEQNVQKSFEQALYLFEVEHNYKASFDEISYALETVEPGVTDRFVTSYE
                     KTQERIRF"
     gene            complement(6191..8143)
                     /gene="gyrB"
                     /locus_tag="VBC74_001284"
     CDS             complement(6191..8143)
                     /gene="gyrB"
                     /locus_tag="VBC74_001284"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEO6942231.1"
                     /translation="MTEETKNMEQRAQEYDASQIQVLEGLEAVRMRPGMYIGSTSKEG
                     LHHLVWEIVDNSIDEALAGFAGHIKVYIEPDNSITVVDDGRGIPVDIQEKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKNGKVHYQEYQRGVVV
                     NDLEIIGDTDLSGTTVHFTPDPEIFTETTVFDFDKLAKRIQELAFLNRGLRISISDKR
                     EGQEVEKEYHYEGGIGSYVEFINENKEVIFENPIYTDGELDGISVEVAMQYTTGYQET
                     VMSFANNIHTHEGGTHEQGFRTALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFNRFLLENPQVAKKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNNAEMNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKVRYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKAYIQPGVNQEEE
                     LRQALDTYSSGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVSVDDAAEADKIF
                     DMLMGDRVEPRREFIEANAVYSNLDI"
     gene            complement(8144..8704)
                     /locus_tag="VBC74_001285"
     CDS             complement(8144..8704)
                     /locus_tag="VBC74_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEO6942232.1"
                     /translation="MNYHDYIWDLGGTLLDNYESSTRAFVETLKEFGYQADHDSVYQK
                     LKESTADAIAYFIPEEADFLKEYKANEAKVLETPILFQGAKELLAKIQRQGSRNFLVS
                     HRDNQVIVILEKTEIIDYFTEVVTADNGFSRKPSPESMLYLKEKYQIDNCLVIGDRDI
                     DKQAGESAGFDTLLVDGSKSLMEIIE"
     gene            complement(8823..10028)
                     /locus_tag="VBC74_001286"
     CDS             complement(8823..10028)
                     /locus_tag="VBC74_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEO6942233.1"
                     /translation="MSQKSNYFKGQIDYAVVIPVFFLLMIGLASIYVATMNDYPSNIY
                     IAMFQQVSWIIMGCIIAFVVMLFSTEFLWKATPYLYALGLTLMVLPLIFYSPQLFAAT
                     GAKNWVTIGSVTLFQPSEFMKISYILMLSRITVSFHQKNRKTFQDDWKLLGLFGLVTL
                     PVMILLMLQKDLGTALVFLAILSGLILLSGISWWIILPILSTIVLFIASFLMIFISPN
                     GKEWFYNLGMDTYQINRLSAWIDPFSFADSIAYQQTQGMVSIGSGGVTGKGFNILELS
                     VPVRESDMIFTVIAENFGFIGSAIVLGLYLIIIYRMLRITIESNNQFYTFISTGFIMM
                     IVFHVFENIGAAVGILPLTGIPLPFISQGGSSLLSNLIGIGLVLSMSYQNTVRQAKAN
                     GSHLKYRSI"
     gene            complement(10453..10641)
                     /locus_tag="VBC74_001287"
     CDS             complement(10453..10641)
                     /locus_tag="VBC74_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO6942234.1"
                     /translation="MSIRTDIDEFKEAIDKGYISGNTVAIVRKNGKIFDYVLLHEEVR
                     EEEVVTVERVLDVLRKLS"
     gene            complement(10683..10883)
                     /locus_tag="VBC74_001288"
     CDS             complement(10683..10883)
                     /locus_tag="VBC74_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO6942235.1"
                     /translation="MSQEKLKSKLDQAKGGAKEGFGKITGDKELEAKGFIEKTIAKGK
                     ELADDAKDAVEGAVDAVKEKLK"
     gene            11061..11532
                     /locus_tag="VBC74_001289"
                     /pseudo
     CDS             11061..11532
                     /locus_tag="VBC74_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3748 a         2290 c         1791 g         3703 t
ORIGIN      
        1 gtttataaaa ttaggaattg caaccttact aacggttatt tcgctttaca caccaataaa
       61 cctagctaca aatcatacca cagaaaatat tgttactgct caagagtata aaacaaaaga
      121 gaatggtact ttacctttta aacacaaacg acaattggtt ttaggagagt tggatgacaa
      181 aggtcgtgcc acattcgcac atattcagct aaaagttaaa gatgaaccca agaaaaagag
      241 agtaaaacgt ttaaaaacaa cccctgttgg atggcacaat tttaaattct attataatga
      301 tggtactcaa aaagcttggc taatgagccg aggccgtctt atttgccacc aatttagtgg
      361 actaaacaat gaaagaaaga atttagttcc aatgacgaat tggctaaata ctggaaacta
      421 caatagcacg aattcatcta accctgagag catgctattc tatgaaaaac aactaaagac
      481 ttggctttcg actcataaaa actactactt agattataaa gtaacaccaa tttatcaaaa
      541 taatgaacta attccaagaa aaattgaatt aaaatatgtt ggtattgata agactgggaa
      601 attattacct atttttatcg gaaacaaatc cactcaagat caattcggca tcagcactgt
      661 taccttagag aatacctcac caaatgctac cattgactac ctcagcggaa aggctcaaaa
      721 tactgtctta tcagctaaag aacaaagaaa acttatcgct aagcacgaag aagaaaaacg
      781 acttgcagaa aagaaagttg aagaggaaaa agctgctgct gaaactcaga aaaaactgga
      841 agaagaacaa gcaaggcttg ctgctgaagc tcaaaggaag caaaaagagg agcaagcaag
      901 gctcgcagct gaaactcaga aaaaacaaga aactctagta caagaacaaa ctagtcaagg
      961 ctacaaacgt gactatagag gaaggtggca tcgtccaaat ggacaatacg cttcaaaagc
     1021 agaaatcgct gctgctggat tgcaatggta gaactgttta ttatcaaatc aattctatta
     1081 ttataatgtg tacataaaaa ccatcttgca tatgcaagat ggtttttatt atattttcaa
     1141 ctgtttaata atctgagact atttttttaa gttgtagaat gatttgatac ctttgtattg
     1201 agcaacttca ccaagttggt cttcgatacg aagtaattgg ttgtatttag caatacggtc
     1261 agtacgtgat aatgaacctg ttttaatttg accagcgtta gttgcaactg cgatatcagc
     1321 gattgttgaa tcttcagttt cacctgaacg gtgtgataca actgcagtgt aaccagcttc
     1381 tttagccatt tcaatagctt cgaaagtttc tgtcaaagta ccgatttggt taactttgat
     1441 aaggattgag ttagcagctt cttctttgat accacgtgct aagtaatcag tatttgtaac
     1501 gaagaaatcg tcaccaacta attgtacacg tccaccaagg cgttctgtaa gagctttcca
     1561 accatcccag tcgttttcat ccataccatc ttcaatagtg atgattgggt atttgttaac
     1621 caactcttca aggtaatcga tttgttctgc agcagtacga actgctccac cttcaccttc
     1681 gaatttactg tagtcgtata ctttacgttc tgcatcgtag aactctgatg aagcacagtc
     1741 aaaaccaatc atgattccgt tttcgccagc ttcgtaacca gctgcttcaa tagctttaag
     1801 aatagtttct acgccatctt cagtaccttc aaatttaggt gcaaatccac cttcatcacc
     1861 aacagctgtt tcaagaccac gttctttaag gattttctta agagcatgga atacttcagc
     1921 accccaacga agtgcttctt tgaaagttgg tgcaccaact ggcataatca tgaattcttg
     1981 gaatgcgatt ggagcgtctg agtgagaacc accgttgatg atgttcatca ttggagttgg
     2041 aagaacttta gtgttgaatc caccaaggta gctgtaaagt ggtacttcaa ggtaatcagc
     2101 tgcagcacgt gctacggcaa tagaaacacc aagaatagca tttgcaccta atttaccttt
     2161 attaggagtt ccgtcaagag cgatcatagc acggtcgata gcttgttggt cgcgtacatc
     2221 ataaccaatg attgcttctg caataacgtt atttacattg tcaacagctt tttgtgttcc
     2281 aagaccaccg taacgagact tgtcgccgtc gcgaagttca actgcttcgt gttcaccagt
     2341 agaagctcct gaaggaacca taccacgtcc gaaagcacct gattctgtat aaacttctac
     2401 ttcaagtgtt gggttaccgc gtgagtcaag gacttcgcgt gcgtaaacat cagtaataat
     2461 tgacattaca atactctcct tatgagtttt aattttttac atattcataa tatcataatc
     2521 tttagttaat aacaaggaaa aacccagtga aaccgcttta ttacgtggtt tttgataaaa
     2581 aaatcactaa ttcttttcaa gtttacctgt gctaatgcaa cccatttagg ttataataaa
     2641 attagcaaat tcaaggagaa attatgactg aacttgacaa taaaatccta caaaaagcaa
     2701 gcggtgaaaa tcgtcttgat cctgatcaac aacgttacta ccttggtact tataaagaaa
     2761 gagttgtgct ccacttaagt ttaaaagagg cacaagaaga aaagtttcaa cttgctataa
     2821 catctgctct taataatctg atacttgaat atcctaatct tagtttaaaa ttatcaccaa
     2881 atctccctac tgatgttcaa atgttctaca tgaagttagc taaccgtcta tcactagctg
     2941 ctacgattgt taatgaagca aatgccaatt ctccatttgg tattattata catagtgata
     3001 aggctgaaaa cgttgaatgg aacgattttg agacacaatt ccctgattta ttcaattcac
     3061 caaaaaaaga ggagtctcct aaaaaaagtc tatggcaaca tttctttagt caaaaataac
     3121 aaggaggtct aatggaaatt tgggatgctt atgaccaata tgggttcatt acaggtctta
     3181 cacttaatag ggatcaaaat atcccacaag gcttattcca cttagttgtt gatgttatcc
     3241 tttttcatga ggatggtgac gtattgatga tgaaacggca ccctaaaaag aaagcttttc
     3301 ctgcttattt cgaagccacc gctggcggat ctgcccttaa aggagaaaat gctaagcaag
     3361 ctattctaag ggaactcaaa gaagaaactg gtatcgttcc tcaatgtcta acctttctta
     3421 atagagagtg gttctctgag cgttcttact ttgtagacca ttttatcgca aaatacaatg
     3481 gtgcaaaaga tataattaca ttgcaagaag gagagaccgt tgactatatc tggttaaaac
     3541 cagaatatat tgatttattt ttatctaaaa acaaattaat accctctcaa ataaaactct
     3601 taaaatcctt aatctaaaaa ctaggcttaa taaaggccta gttttcattt gcatcaataa
     3661 tatcaaatac cttaagcaaa tttttttcat taatctgata cgcagcttct tctctgacaa
     3721 ttggttttgc acaaaaagca attcctaccc cagcggattt gatcattggt aaatcattgg
     3781 caccatctcc cattgcgatg gtttgtgaaa ggtctaagtg attttcagaa gcccactctt
     3841 ttaactttat ttttttcact tctttagtga caatctctcc ctcaacttcg ccagtgagaa
     3901 aaccaccttt gacaccaaga cgattcgctt tgacataatc aacttgtaat tgcttagcta
     3961 agatatcaat cgtctcgtga aaacctcctg atacaagacc aactttcatc tgacgcttgt
     4021 gacactcttt aatcaattca taagcccctg gagtaaaatg tattgaggat aatatatctg
     4081 tcaaaatagt aacaggcaaa cctttaagta gcgctactct tttcttcaaa gcttcttcaa
     4141 aatctaactc gccacgcatt gcagcgtcag tcagtgcagc aacctgtttc cccacccctg
     4201 cttcaatagc taataaatca atagcttctt ccattattaa ggttgaatca acgtccatga
     4261 ctaacaaacc tttaatcatt taaaacctcc aaaacttttt tactattgta ccttaaaatt
     4321 agcacctttc atagatagca attaaaaaaa gaagagtact aactcttctt ttttagaaac
     4381 gaatacgttc ttgtgtcttt tcataagacg tcacaaaacg gtcagttaca cctggttcca
     4441 cagtctcaag tgcgtatgaa atttcatcga aagatgcttt atagttatgt tcaacttcaa
     4501 ataagtacaa agcttgttca aagctctttt gcacattttg ttcaaaacta cgataacggt
     4561 ttgaatactg tagtaactgc tctgtgagag tcgcatcttg aacaactaga tatgtagctt
     4621 gctctaaatt agcgatagca tttgtagtca catcatttaa acgtgaaaca gcctcaatat
     4681 caatacgacc gcgactgagt tcattaataa gtgcctcaat ttgagaactt gttgtaaaga
     4741 aagtgcttaa gaagtcttgc gggattcctg gaagattgcg tttttccatg aaacgtttaa
     4801 tcatatggag cttgttaaca tacttatcca tattttggcg tgctcgtgtc tcaacgcttt
     4861 caatagtttt aacggcttca aaaacttcca tttgattttc ttcaacagta gtcaatgttt
     4921 tcaacgtacg ctcaaataaa atttgtagat gagtataagg tgaagcttct tgaccaaaat
     4981 tttctacaga aggtaaaacc tttgtttcaa tttcttccaa atcatttgtg aaggaacgta
     5041 aacttaagct ttcattttca tttaagatat atttagcaga taatcgtttg atttcatgcc
     5101 ccagttgttc attgttagat tttgcatgag caaggaattg aggaataatt ttactatcct
     5161 tatgagctac tttataagca gcaatctcac gttcaaaaat atcataaagt ttatcaattt
     5221 tttcttgaat atgttcattt tcatcgcgag cacgatctag atccaaacta actaaaccat
     5281 ctgaattcga acgaatagcc tcacgaactt cttgaaaacg ttgttcaata tccttttcag
     5341 ggaaatggta gttttcctct aaaagacgac gatatcctgt ctccaaatca tctaactgat
     5401 ctggaaaatc gtcttctaat ttagcaacaa tagctggaat ttgttctgta atttgcccta
     5461 aagcaatcgt atgctcctct gctttattca aaacttcaga agcttcaatc gggtcaccag
     5521 ttgagtttaa ggtcacaaaa tgtgaaaatt cagcctcgat atttttcaac tgcttttcaa
     5581 tttctggcat ggttgtcccg taattatctt ctttttcaga aattgatttt tgaagtgttt
     5641 cgtataggtc caaagcatgt gttacacggg cactattttt ctcttcttgc tcttttaaaa
     5701 cttctaaagc ttcacgaatc gaaacaatat cttcttctat aatagttagc tgactatcaa
     5761 cattatcaat ctcgtgctta gcacgcacaa aattaaatga atcattaaga ttttcagctt
     5821 caactaaatg aaaatcaata tctttaaatg aattagttga taaatcagtc catttttggt
     5881 tccattctct aaaagttgat tgactttggc caatcaaatg aagcaatttg acttgctcaa
     5941 tctcctcttg cacaggaagg tcaactaact cttgttttct agtttctaag tttgcaatta
     6001 atgtatcatt acgttttctt attacgacgc cgactacgta ggcaataatc acaagcagga
     6061 ctatagccac aagcagcaat atgattccgc tcgacattca actctcctta taatggtcta
     6121 attttctaaa aatctactat aacgttaaga ttatagcata cttgaactgt ttttcaaagc
     6181 tatctttgga ctagatatct aagttactat aaacagcatt agcttcaatg aattcacgtc
     6241 gtggttctac tcgatctccc attagcatat cgaaaatctt atctgcttct gctgcatcat
     6301 caactgagac acgtgccatc aagcggtttt caggatccat tgttgtttcc caaagttgat
     6361 ggtcatccat ctcacctaga cctttgtaac gttgtacagt aggttttgaa cgtcctgaac
     6421 tataggtatc tagagcttga cgtaattcct cttcttgatt gacccctggc tgaatataag
     6481 ctttaatctc acttcctact ttgacaccat agattggtgg ttgagcgata taaacatatc
     6541 ctgcttccaa aacaggtcgc ataaaacgat aaatcagagt taaaagtaaa gttctgatgt
     6601 gagctccatc aacatcggca tcagtcataa tgactaattt ttgatatctg acttttgata
     6661 catcaaaatc tgcaccaaat ccagttccca tagctgtgaa gagacttcgg atttcttcgt
     6721 ttgctaaaat tttatccatt gtagcttttt caacgtttag aatcttaccc ctaatcggta
     6781 aaattgcttg aaactcacga ttacgtcctg attttgcaga ccctcccgca gaatctccct
     6841 ccactataaa aagttcattc atctcagcat tattagatga gcaatcagcc agtttcccag
     6901 gtaagtttga aatctctaag cctgactttt tacgtgttac ttcacgcgcc cgttttgctg
     6961 caatacgggc tttagatgcc aaaattcctt tttcaacaat cttcttagct acttgtggat
     7021 tttctaatag gaatcgattg aaagcttcgc taaagagacg atttgtaatt ttaacaactt
     7081 cagaatttcc aagttttgtt ttagtttgtc cttcaaattg tggatttgga tgtttaacag
     7141 atataactgc tgttaaacct tcacgaacat cttctccagt taaattatct tcattttctt
     7201 tgagaatctt attcttttta gcgtaatcat taatcacacg tgtcaaggca gtacgaaatc
     7261 cttgttcgtg cgttcctcct tcatgagtgt ggatgttatt tgcaaaactc ataacagtct
     7321 cttgatatcc agtagtatac tgcattgcta cttcaactga aataccatct aattcaccgt
     7381 cagtataaat aggattttca aaaatcactt ctttgttctc attgataaac tcaacgtatg
     7441 aacctatacc accttcatag tgatattctt tttcaacttc ttgaccttct cgcttgtctg
     7501 aaatggagat tcttagtcca cgatttaaaa aagctaattc ctgaatacgt ttggctaatt
     7561 tatcaaaatc aaatacagtg gtttcagtga aaatttctgg gtcaggcgta aagtgaactg
     7621 ttgttcctga taagtcagta tcaccgataa tttcaagatc attaacaaca acaccgcgtt
     7681 ggtattcttg ataatgaacc ttgccatttt tataaacctt gacatctaat tgtgttgaca
     7741 atgcattaac aacagatgac cctacaccat gtaagccacc agaaaccttg tatccgccac
     7801 caccaaattt accaccggca tggagaactg taaaaactgt ctctacagct ggtcttccag
     7861 ttttttcttg gatatctacc ggaataccgc gaccatcatc aacaactgta atagagttat
     7921 ctggttcaat gtaaacttta atatgtcctg caaatccagc taaggcttca tcaattgagt
     7981 tatcaacaat ttcccataca aggtgatgaa gcccttcttt cgaagtcgaa ccgatataca
     8041 tgcctggacg catgcgaaca gcttctaaac cttctaatac ttgaatctga cttgcgtcat
     8101 attcttgagc acgttgttcc atattctttg tttcttctgt catttactct attatctcca
     8161 tcaatgattt tgacccatcg actaataagg tatcaaaacc agccgattct cctgcttgct
     8221 tatctatatc acgatcgcct attactaaac aattatcaat ttggtatttt tctttcaaat
     8281 aaagcatact ctcgggacta ggtttccttg aaaagccgtt atcagcagtc actacttctg
     8341 taaaatagtc aataatttct gttttttcca gaataactat aacttgatta tcacgatgag
     8401 acaccaaaaa attacgacta ccttgtcttt gaattttcgc taaaagttct tttgcccctt
     8461 ggaatagaat tggtgtttcc aaaaccttag cttcattagc cttatactct tttaaaaaat
     8521 cagcttcttc agggataaaa tatgcaattg catctgctgt tgactctttt aatttttgat
     8581 acacactatc gtggtctgct tgatatccaa attctttcaa agtctctaca aaagcacgtg
     8641 tggaactttc gtaattatca agtagtgtac cacccaaatc ccaaatataa tcatgataat
     8701 tcataccctt tattatatca tattattacg attttttata gctaccacct tacttaatta
     8761 actcactcct ctaaaatatt tcgattatta aaaggctgga ttatctttcc aacctttttt
     8821 tgttatatac tacgatactt caaatgtgat ccattagcct tggcctgacg aactgtattt
     8881 tgataagaca tggataagac caatccaatt ccaataagat tacttagtaa tgaacttcca
     8941 ccttgcgaaa taaacggaag tgggattcct gttagaggta atataccaac tgctgcacca
     9001 atattttcaa aaacatggaa aacaatcatc atgataaaac cagttgatat aaaagtataa
     9061 aattgattat tagattcaat ggtgatcctt aacatacggt aaatgataat gagatataac
     9121 cctaaaacaa tcgccgaacc aatgaagcca aaattctcag caatgacagt aaaaatcatg
     9181 tcactttcac gaacaggaac cgagagctct aaaatattaa aacctttacc tgttaccccg
     9241 ccacttccaa ttgataccat accctgtgtt tgttgataag caattgagtc tgcaaaacta
     9301 aatggatcaa tccaagcaga taaacggttg atttgatatg tatccatccc caaattataa
     9361 aaccattcct tgccgtttgg tgagataaaa atcatcaaaa agctagctat aaaaagaacg
     9421 atagtagata aaattggcaa gattatccac caagatatgc ccgataaaag gattaaacct
     9481 gacaaaatgg caaggaaaac taatgctgtt cctaaatctt tttgtaacat tagaagaatc
     9541 atcactggca atgtaaccaa accaaacaat cctagcaact tccaatcatc ttgaaaagtt
     9601 ttgcgatttt tctgatgaaa actaacagtt attcgagata acattaaaat ataggaaatc
     9661 ttcataaact ctgacggttg aaacaaagtt accgagccaa tggtcaccca atttttagca
     9721 cctgttgccg caaaaagctg tggactataa aaaattaagg gaagaaccat tagggttagt
     9781 cctaaagcat acagataggg cgtggctttc cataaaaatt cagtactgaa aagcataacg
     9841 acaaatgcaa taatacaccc cataataatc caagacacct gctgaaacat cgcgatatag
     9901 atattagaag gataatcatt catcgttgca acataaattg aggctaaacc aatcattaat
     9961 aaaaaaaata ctggtatcac aacagcataa tcaatttgcc ctttaaaata gtttgatttt
    10021 tgtgacatag gacttccttt tttattcatg gtaattatta taacattttt tcagaatctg
    10081 acttatattt tttgcaataa aaaatgcaac tcaaatcatt atgagttgca aatattagga
    10141 gattgttctt catcagtaaa acaaagaaca tgaaaagaaa agtttattta ggatatttaa
    10201 agtatacaaa gttcacctta aacaaaactt aaataatctt atttaagttt tgttattttc
    10261 tcaacactag tctaaagaat aaaatccttc ctaacatctc aaaaaaggtt ttaatatgtc
    10321 ccctgccaac ttctctacca ttaaaatagg atatattaaa ggttaaaata cacgatacat
    10381 ccataataaa aagcatattt ttttatttta ttctatttta aaatactttt tggggcgaat
    10441 tttggtccgt tattatgata acttcctcag tacatcaagc actctctcaa ctgtaacaac
    10501 ctcttcttct ctcacttctt cgtgtagtaa cacataatca aatatctttc cgtttttacg
    10561 cactatcgct actgtgttcc ctgaaatata gcctttatca atcgcttctt taaactcatc
    10621 tatatctgtt ctgatagaca aaaagataac aaataaccgc tcgttaaatg agcggttatt
    10681 tgttacttca gtttttcttt gacagcatct actgcccctt caacagcatc tttagcatca
    10741 tctgctagtt ctttgccttt agcaattgtt ttttcgataa atccttttgc ttctaactct
    10801 ttatcaccgg ttattttgcc aaagccttct ttagcaccac cttttgcttg atctaatttt
    10861 gattttagtt tttcttgtga catactgcac ctccttatta tttttattaa tcataacatc
    10921 tatatctttc tttagcaaat aaaaacaaga gtcgctagtg tcaggcgact cttgttttag
    10981 tgattatctc atggttatgc gagtatggat gtatactata aaaatagaca acactttgtg
    11041 tgttataacc tgttttaaaa gacacaaaaa tctctgaagg aagaatttac cgtctgatga
    11101 agaatatggc gctccctaaa atggctaccg ttaagacaaa aacagctctt aaaaagactc
    11161 aaaaaacgta tcctcaaaac ttactcaacc agaaatttta tcctgataaa cctaatcaag
    11221 tatggtctac tgacttcacc tatatttcta ttggatataa gaaatatgtc tatctctgcg
    11281 caataattga tctctattct agaaaatata ttgcttggaa attgagtcat cgtatggatg
    11341 caaagttagc atgtgacact ctagaattag ctcttaataa aagaaagatt gaaggaacac
    11401 ttctctttca ttccgaccaa gggtcacaat ttaaggccag ggaatttaga aaaataattg
    11461 atgacaacaa tatcatgcat tctttttcta aacctagata tccttatgat aatgccgtaa
    11521 ctgaagcatt tt
//
LOCUS       DATROD010000054        11307 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.054, whole genome shotgun sequence.
ACCESSION   DATROD010000054 DATROD010000000
VERSION     DATROD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11307
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.054"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            100..1794
                     /locus_tag="VBC74_001290"
     CDS             100..1794
                     /locus_tag="VBC74_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001106850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEO6942236.1"
                     /translation="MNYKEIYQEWLENDSLGKDIKSDLEAIKGDESEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAIARGIAVSYDVRYQSKEFA
                     ELTCSIMAANGIKSYIYKGIRPTPMCSYAIRALGCVSGVMVTASHNPQAYNGYKAYWK
                     EGSQILDDIADQIANHMDAITDYQQIKQIPFEEALASGLASYIDESIEEAYKKEVLGL
                     TINDTNIDKSVRVVYTPLNGVGNLPVREVLRRRGFENIYVVPEQEMPDPDFTTVGYPN
                     PEVPKAFAYSESLGKSVDADVLLATDPDCDRVALEVKDSKGEYIFLNGNKIGALLSYY
                     IFSQRCALGNLPHHPVLVKSIVTGDLSKVIADKYNIETVETLTGFKNICGKANEYDIS
                     KDKTYLFGYEESIGFCYGTFVRDKDAVSASMMVVEMTAYYKERGQTLLDVLQTIYDEF
                     GYYNERQFSLELEGAEGQERISRIMEDFRQNPILQVGEMTLENSIDFKDGYKDFPKQN
                     CLKYYFNEGSWYALRPSGTEPKIKCYLYTIGCTEADSLSKLNAIESACRAKMNSTK"
     gene            1933..2787
                     /locus_tag="VBC74_001291"
     CDS             1933..2787
                     /locus_tag="VBC74_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEO6942237.1"
                     /translation="MTELIDGKALSQKMQAELGRKVERLKEQHGIIPGLAVILVGDNP
                     ASQVYVRNKERSALEAGFKSETLRLSESISQEELIDIIHQYNEDKSIHGILVQLPLPQ
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPMMVPCTPAGIMEMFREYHVDLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRNLSEVTKEADILIVAIGQGHFVTKD
                     FVKEGAVVIDVGMNRDENGKLIGDVVFEQVAEVASMITPVPGGVGPMTITMLLEQTYQ
                     AALRSVSL"
     gene            2784..3620
                     /locus_tag="VBC74_001292"
     CDS             2784..3620
                     /locus_tag="VBC74_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEO6942238.1"
                     /translation="MIVGEQEARALIKPRPKSSHKGDYGSVLLIGGFYPYGGAIIMAA
                     LACVKTGAGLVTVATQSCNIPSLHSQLPEVMAFDSDDYKWLEKSIVQSDVIVIGPGLG
                     VSESSRKILNRTMEKIQSHQSVIIDGSALTLLSEGAFPQTKAKNLVLTPHQKEWERLS
                     GIAVSQQTKENTQTALKSFPKGTILVAKSSHTRIFQDLDEKEIIVGGPYQATGGMGDT
                     LCGMIAGMLAQFKEASPLDKVSVGVYLHSAIAQELSKEAYVVLPTTISDEIPKEMARL
                     SK"
     gene            3746..5086
                     /locus_tag="VBC74_001293"
     CDS             3746..5086
                     /locus_tag="VBC74_001293"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEO6942239.1"
                     /translation="MSDYLSVSTLTKYLKLKFDKDPYLERVYLTGQVSNFRRRPNHQY
                     FSLKDDKSVIQATMWSGQFKKLGFELEEGMKVNVVGRVQLYEPSGSYSIIVEKAEPDG
                     IGALAIQFEQLKKKLSQAGYFDDRHKQLLPQFVRKIGVVTSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGEGAAQEIAQTIALANEKKDLDLLIVGRGGGSIEDLWAFNEECVV
                     EAIFESRLPVISSVGHETDTTLADFVADRRAATPTAAAELATTVTKIDILSWITEREN
                     RMYQSSLRLIRTKEERLQKSKQSVIFRQPERLYDGFLQKLDNLNQQLTYSMRDKLQTV
                     RQKQGLLHQKLQGIDLKQRLHTYQERVVQSRRLLSSTMTSQYDSKLARFEKAQDALIS
                     LDSSRIVARGYAIIEKNHTLVSTTNGINEGDHLQVKMQDGLLEVEVKDVRQENI"
     gene            5064..5279
                     /locus_tag="VBC74_001294"
     CDS             5064..5279
                     /locus_tag="VBC74_001294"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEO6942240.1"
                     /translation="MSDKKTFEENLQELETIVSRLETGDVALEDAIAEFQKGMLISKE
                     LQKTLKEAEETLVKVMQADGTEVEMDT"
     gene            5279..6151
                     /locus_tag="VBC74_001295"
     CDS             5279..6151
                     /locus_tag="VBC74_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEO6942241.1"
                     /translation="MVTIEKIDEAIHRHYKQTHSVVSPDLIKAILYSVDGGGKRIRPR
                     ILLEILEGFGVELIDGHYDVAAALEMIHTGSLIHDDLPAMDNDDFRRGRLTNHKKFDE
                     ATAVLAGDSLFLDPFDLVVKAGFKADVTVRLIELLSMSAGSFGMVGGQMLDMKGENKV
                     LSIDDLSLIHINKTGRLLAYPFVAAGILAEKSEEVKGKLHQAGLLIGHAFQVRDDILD
                     VTASFEELGKTPNKDIVAEKTTYPNLLGLDKSQEILDDTLKKAQAIFQNLEKKANFNA
                     RKIIDIIEGLRLNG"
     gene            6144..6971
                     /locus_tag="VBC74_001296"
     CDS             6144..6971
                     /locus_tag="VBC74_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEO6942242.1"
                     /translation="MAKERVDVLAYKQGLFDTREQAKRGVMAGLVINVINGERYDKPG
                     EKVADDTELKLKGEKLKYVSRGGLKLEKALQVFEISVADKLTIDIGASTGGFTDVMLQ
                     SGARLVYAVDVGTNQLVWKLRQDHRVRSMEQYNFRYAQKEDFKEGLPEFASIDVSFIS
                     LNLILPALKEILVDGGQVVALIKPQFEAGREQIGKNGIVKDKLVHEKVLTTVTNFTKD
                     YGYTVKHLDFSPVQGGHGNIEFLMHLQKCQDPQNLVLDQIQDVIEKAHKEFKKNEEE"
     gene            6958..7431
                     /locus_tag="VBC74_001297"
     CDS             6958..7431
                     /locus_tag="VBC74_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEO6942243.1"
                     /translation="MKKSERLNLIKQIVLNHAVETQHELLRRLEAYGVTLTQATISRD
                     MNEIGIIKVPSAKGRYIYGLSNENDPIFTTAVAKPIKTSILSISDKLLGLEQFININV
                     IPGNSQLIKTFIMSHCQEHIFSLTADDNSLLLIAKSEADADHIRQSMIAMLEKKD"
     gene            7443..9101
                     /gene="recN"
                     /locus_tag="VBC74_001298"
     CDS             7443..9101
                     /gene="recN"
                     /locus_tag="VBC74_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEO6942244.1"
                     /translation="MLLEISIKNFAIIEEISLNFETGMTVLTGETGAGKSIIIDAMNM
                     MLGARASVEVIRHGANKAEIEGFFSIEKNQSLVQLLEENGIELADELIIRREIFQNGR
                     SVSRINGQMVNLSTLKAVGHYLVDIHGQHDQEELMKPNMHILMLDEFGNTEFNVIKER
                     YQSLFDAYRQLRKRVLDKQKNEQENKSRIEMLEFQIAEIESVALKSDEDQTLLKQRDK
                     LMNHKNIADTLTNAYLMLDNEEFSSLSNVRSAMNDLMALEEFDREYKDLSTNLSEAYY
                     VIEEVTKRLGDVIDDLDFDAGLLQEIENRLDVINTITRKYGGDVNDVLDYFDNITKEY
                     SLLTGSEESSDALEKELKILEHDLIESANQLSLERHKLAKQLENEIKQELTELYMEKA
                     DFQVQFTKGKFNKEGNEIVEFYISTNPGEGFKPLVKVASGGELSRLMLAIKSAFSRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIHKIGSHGQVLAISHLAQVIAIADYQYFIEKIS
                     SDSSTVSTVRLLSYEERVEEIAKMLAGNNVTDTARTQAKELLGS"
     gene            9214..10050
                     /locus_tag="VBC74_001299"
     CDS             9214..10050
                     /locus_tag="VBC74_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEO6942245.1"
                     /translation="MSKIKIVTDSSITIEPELIKELDITVVPLSVMIDGTLYSDNDLK
                     AQGEFLNLMRGSKELPKTSQPPVGVFAEIYEKLMNEGAEHIIAIHLTHTLSGTIEASR
                     QGANIAGADVTVIDSTFTDQCQKFQVVEAAKLAKEGADLDTILARVEEVRQKSELFIG
                     VSTLENLVKGGRIGRVTGLLSSLLNIKVIMELTNHELVPIVKGRGLKTFSKWLDNFVE
                     SAQTRKIAEIGISYCGKADMANNFKEKLAVLGAPISVLETGSIIQTHTGEDAFAVMVR
                     YE"
     gene            10043..10882
                     /locus_tag="VBC74_001300"
     CDS             10043..10882
                     /locus_tag="VBC74_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEO6942246.1"
                     /translation="MNKKKILTGLSFFLVSLLLSFGIFSLIIPKSNPKLTKKDFLTKK
                     VIPLNYVALGDSLTEGVGDTTSQGGFVPLLSESLHNRYSYQVTSVNYGVSGNTSQQIL
                     KRMTTDPQIEKDLEKADLLTLTVGGNDVLAVIRKELSHLSLNSFEKPAEAYKERLKEI
                     LAKARQDNPKLPIYVLGIYNPFYLNFPQLTKMQTVIDNWNKATKEVVDASENVYFVPI
                     NDRLYKGINGKEGITESSNSQASITNDALFTGDHFHPNNIGYQIMSNAVMEKINETRK
                     NWP"
     gene            10857..>11307
                     /locus_tag="VBC74_001301"
     CDS             10857..>11307
                     /locus_tag="VBC74_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEO6942247.1"
                     /translation="MKQEKTGRNLNFWKWAFLLLLAINLSFTAVIASRLIQVREPNTG
                     KISTGVQDKVKVGTFTTNKSQLNKTIALYLKQYQTKKMNYKIYAASSSILFEGSYQLL
                     GYEVPLYIYFEPYRLTNGAVQLKVTSFSVGTLPLPEKDVLQYIKSSYK"
BASE COUNT         3803 a         1703 c         2350 g         3451 t
ORIGIN      
        1 aaggttaatt tgatactatt cagttagctc ctgaagaact aaaatctctt atcatggagc
       61 tgatttttac cattttggtc ttagaatagg atgtctcata tgaattataa agagatttat
      121 caagagtggt tagaaaacga ctcactcggt aaagatatta agtcagattt agaagctatt
      181 aaaggagatg aatctgaaat tcaggatcgt ttttacaaaa cattagaatt tggaacggcg
      241 ggattgagag gtaaacttgg agcaggaacc aatcgtatga atacttatat ggtggggaaa
      301 gcagcacaag cattagctaa tacgattatt gatcatggcc ctgaagctat tgcacgtgga
      361 attgcagtta gttatgatgt ccgttatcaa tctaaggaat ttgcagaatt aacttgttcc
      421 attatggcag caaatggtat taagtcttat atttataaag ggattcgccc aacaccaatg
      481 tgctcatatg ctattcgtgc tctaggatgt gtttcgggtg tgatggttac tgctagtcat
      541 aatcctcaag cttataatgg ttataaggca tattggaaag aaggatctca gattttagat
      601 gatattgctg accaaattgc caatcatatg gatgctataa ccgattatca gcaaattaag
      661 caaataccct ttgaagaggc tctggcaagt ggtttggcaa gttatattga tgagagtatt
      721 gaagaagcat ataaaaaaga agtgcttggt ttaactatta atgatactaa cattgataag
      781 tcagtccgag tagtttatac tcctttaaat ggcgtaggaa atttacctgt gcgcgaagtt
      841 ttaagacgcc gtggttttga aaatatttat gtagtacctg agcaggaaat gcccgatcct
      901 gattttacaa cagttggcta tccaaaccct gaagttccta aagcatttgc ctattcagaa
      961 tctctaggaa agtcagttga tgcagatgtc ttacttgcca cagatccaga ttgtgaccga
     1021 gtagcattgg aagtcaaaga tagtaaggga gaatatattt tcttaaatgg taataagata
     1081 ggggcacttc tttcctatta tattttttca caacgatgtg ccttagggaa tttgccacat
     1141 catcctgtat tggtaaaatc cattgtaact ggtgatctat caaaagttat tgcagataaa
     1201 tataatattg aaactgttga aactttaaca ggatttaaaa atatttgtgg aaaagctaat
     1261 gaatatgata tctcaaagga taaaacttat ctctttggct atgaagaaag tattggtttt
     1321 tgctatggca cttttgtacg tgataaagat gctgtgagtg cttcaatgat ggtagtagaa
     1381 atgactgcct attataaaga acgagggcaa acacttttag acgttttgca aaccatttac
     1441 gatgaatttg gctattacaa cgagcgccaa ttttctcttg agttagaggg tgctgagggg
     1501 caagaacgta ttagtcgtat tatggaggat tttagacaga acccaatatt acaagtaggt
     1561 gagatgacat tggagaattc tattgatttc aaggatggtt ataaggattt tccaaagcaa
     1621 aattgtttaa aatattattt taatgagggt tcatggtatg ctttaaggcc gtcagggaca
     1681 gaacctaaga taaaatgtta cctttatacg attggttgta cagaagcaga tagtttatcg
     1741 aaacttaatg caattgagtc ggcttgtcgt gctaaaatga atagtactaa ataagtcata
     1801 tgtaaaaagg cgatgaggga ttcattgcct tttctattga ttaaaattga tacaaaatga
     1861 atagcgatat aaaatagcat aatcgctatt ttttgatata atatagaaaa aacggatgaa
     1921 gaaggagtat caatgactga attaatagat ggtaaagcct tatcacaaaa aatgcaggct
     1981 gagttagggc gaaaagttga aaggttaaag gaacagcatg gtatcatacc tggtttagct
     2041 gtaatccttg ttggggataa tccagctagc caagtgtacg ttcgaaataa agaacgctct
     2101 gcattagaag ctggttttaa aagtgagact ttacgattat ctgaatccat ttctcaagaa
     2161 gaactgattg atattatcca tcaatacaat gaagataaaa gtatccatgg tattttagtt
     2221 caacttccgt taccacaaca tattaacgat aaaaaaatta ttttagcaat agatcctaag
     2281 aaagatgttg atggtttcca cccaatgaat acgggtcatc tttggtcagg acgaccgatg
     2341 atggtacctt gtacgccagc tggtattatg gagatgttta gagagtacca tgtggattta
     2401 gagggaaaac atgctgttat catcggaagg tccaatattg ttggtaagcc aatggcacaa
     2461 cttctccttg ataaaaacgc aacagttact ttgacacatt caagaactcg aaacctttct
     2521 gaggtaacaa aggaggctga tatccttatt gttgcgattg gtcaggggca ctttgttaca
     2581 aaagacttcg ttaaagaagg tgctgttgtg attgatgttg gtatgaatcg cgatgaaaat
     2641 ggtaaattga ttggagacgt tgtatttgaa caagtggcag aagttgctag tatgataaca
     2701 cctgttcctg ggggcgtagg acctatgacg attacaatgc ttttggaaca aacttatcaa
     2761 gcagctctta gaagtgtgag tctatgattg ttggtgaaca agaagcgagg gcgttaatta
     2821 agccacgccc taaatcaagc cataaaggtg attatggtag tgttcttctg ataggaggtt
     2881 tttatcccta tggaggtgct attataatgg cagctttggc ctgtgtcaaa actggtgcag
     2941 gattagttac tgtagcaacc caaagttgca atatcccctc tttgcatagt caactaccag
     3001 aggtaatggc gtttgatagt gatgattaca aatggttgga aaaatcaatt gttcaaagtg
     3061 atgttattgt aattggtcct ggattaggag tatcagaatc atctcgaaaa attttgaacc
     3121 ggaccatgga gaagattcaa tcacatcaaa gtgtcatcat tgacggatca gccttgactc
     3181 tgttatcaga aggtgcgttt ccgcaaacaa aggctaagaa tttagtgttg acacctcatc
     3241 aaaaagaatg ggagcgattg tcaggtattg ctgtatcgca acagacaaaa gaaaataccc
     3301 aaaccgctct taaatctttt cccaaaggga cgatcttagt cgctaagagt tcgcatacgc
     3361 gtatttttca agatttagac gaaaaagaaa ttatagtagg aggtccttac caggcgactg
     3421 gagggatggg ggatactttg tgtggtatga ttgcaggtat gttagcgcaa tttaaagaag
     3481 cctctcctct agataaggta tcagtgggag tttatctaca ttcggcaatt gctcaagaat
     3541 tatctaaaga agcctacgtt gttctaccga caacgattag tgatgagatt ccaaaagaaa
     3601 tggctagact atctaaataa aaaataagta gagaggtgtt agcatgacac ttcttttgct
     3661 ttatcgagct atttgtagag caaataggta ttttttaagt ttttttggta taatgaagtt
     3721 aataaaactc tcatgaggag gtgctatgtc agactattta tccgtgtcga cactgactaa
     3781 gtatttaaaa ttaaaatttg acaaagaccc ttatttagag agggtttatt taactgggca
     3841 agtgtctaat tttcgtcgtc gcccaaacca tcaatacttc tctttaaaag atgataaatc
     3901 agtcattcag gcgacaatgt ggtcaggtca attcaaaaaa ctgggttttg aattagagga
     3961 agggatgaag gttaatgtag ttggccgtgt tcaactttat gaaccaagcg gctcctactc
     4021 tattattgtt gaaaaagcag aaccggatgg tataggagct cttgctatcc aatttgaaca
     4081 actaaagaag aaactctctc aagcaggtta ctttgatgat cgtcataagc aattacttcc
     4141 tcaatttgtt agaaaaatag gagttgtgac tagtccaagt ggagctgtta ttcgggatat
     4201 tattacaaca gtatctcgtc gattcccagg agtggaaata ttacttttcc ccacaaaagt
     4261 tcaaggagag ggcgcagctc aggagatagc acaaactatt gctttagcta acgagaaaaa
     4321 ggatttggat ctattaattg ttggccgtgg gggaggttct attgaggact tatgggcatt
     4381 taatgaagaa tgtgttgtag aagctatttt tgaatctcgt cttcccgtta tttctagtgt
     4441 cggacatgaa actgatacaa ctttagcaga ttttgtagcc gatcgcagag cagcaactcc
     4501 aactgcagca gctgagcttg ctacaacggt tactaaaatt gatatcttgt cttggattac
     4561 tgaacgtgaa aatagaatgt atcagtctag ccttcgtctt atcaggacaa aagaggagag
     4621 gttgcaaaaa tcaaagcaat cagttatttt taggcaacca gagcgtctat atgatggttt
     4681 ccttcaaaaa ttagataacc taaatcagca gttaacatat tctatgcgtg ataaactaca
     4741 aacagtaaga caaaagcaag gtttgcttca tcaaaaactg caaggcatag atttaaaaca
     4801 gcggcttcat acttaccaag agcgtgttgt gcaaagtaga cgactattat caagtacaat
     4861 gactagtcag tatgacagca agctagcacg ttttgaaaag gctcaagatg cgcttatatc
     4921 attggatagt tctagaattg tagcgcgtgg ttacgctatt atcgaaaaaa accatacttt
     4981 agtatctact actaatggaa taaatgaagg agatcatttg caagttaaga tgcaagatgg
     5041 tcttttagaa gttgaggtga aggatgtcag acaagaaaac atttgaagaa aatttgcagg
     5101 aattagagac aattgtctca cgtttagaaa caggtgatgt tgccttagaa gatgccattg
     5161 ctgaatttca aaaaggaatg cttatttcaa aagagttaca aaaaacttta aaagaagctg
     5221 aagagacgct tgtaaaagtg atgcaagctg acggaacgga agtagaaatg gatacttgat
     5281 ggttactatt gaaaaaattg atgaagctat tcatcgtcat tacaaacaga cacatagtgt
     5341 cgtttctcct gatttaatta aggctatcct ctactcagtt gatggaggtg ggaaacgtat
     5401 tcgtccacgc atccttttag aaattttaga gggatttgga gttgaactaa tagatggtca
     5461 ttatgatgtg gctgctgctt tggagatgat tcacactggc tctttaattc atgatgattt
     5521 accggcgatg gataatgatg attttcgtcg tggccgctta acaaaccata aaaagtttga
     5581 tgaagctaca gcggttttag ctggagattc ccttttttta gacccatttg atttagttgt
     5641 aaaagctggt tttaaagctg atgttactgt taggttaata gagttattgt ctatgtctgc
     5701 gggtagtttt ggcatggttg gtggacaaat gttagatatg aagggagaaa acaaagtttt
     5761 atctattgac gatttaagct tgattcatat taataaaacg ggacgcctgt tagcttatcc
     5821 ctttgttgca gcaggtattt tagctgagaa gtcggaagaa gtaaaaggaa aactgcatca
     5881 agctggcctt ttaatcggtc atgcttttca agtacgtgat gatattttag atgtgactgc
     5941 tagttttgaa gaattgggta agacaccaaa taaagacatt gtagcagaaa agacaactta
     6001 tccaaattta ttgggtttgg ataagtcaca ggaaatactt gacgatactt tgaaaaaagc
     6061 tcaggcaatt tttcaaaatc tagagaaaaa agctaacttt aatgctagaa aaataataga
     6121 tataatagag ggattacggt tgaatggcta aagagagggt agatgttctt gcctataaac
     6181 agggactttt tgatacacga gagcaagcga aacgtggtgt tatggcagga ctggtgatta
     6241 acgttatcaa tggagaacgt tatgataaac caggcgaaaa ggttgcagac gatactgaat
     6301 taaaactaaa aggtgaaaaa ctaaaatatg ttagtagagg tggattgaaa ttagaaaaag
     6361 ctttacaagt ttttgaaatt tcagttgcag ataagctaac tatagatatt ggcgcctcta
     6421 cgggtggttt tactgatgtt atgctacaat caggagcgcg tttagtttac gcagtagatg
     6481 taggaacaaa tcaattagtt tggaagttac gtcaggatca tcgtgttcgt tctatggaac
     6541 aatataattt taggtatgcc caaaaagaag atttcaagga gggactgcct gaatttgcat
     6601 cgatagatgt ctcatttatc tctcttaatt tgattttacc agctctaaaa gaaattttag
     6661 tggatggtgg acaagtagtg gcattaatta aaccacaatt tgaagcaggt cgtgagcaaa
     6721 ttggtaaaaa tggtattgtc aaagacaagt tggttcatga aaaggttttg acaacagtga
     6781 ccaatttcac gaaagattat ggatatacgg ttaaacatct tgatttttcg cccgttcaag
     6841 gtggacatgg aaatattgag tttttaatgc atttgcaaaa gtgtcaagat ccacaaaatc
     6901 ttgtgcttga ccaaatacaa gatgttatag aaaaagcaca taaggaattt aagaaaaatg
     6961 aagaagagtg aacgtttaaa tttaattaag caaattgttc ttaaccatgc tgttgaaaca
     7021 caacatgagt tgttacgcag attggaagct tatggggtaa ctctaactca agcaactatt
     7081 tcacgtgata tgaatgaaat tggcattata aaagtgccat cagcaaaagg tcgctatatt
     7141 tacggtttgt caaatgaaaa cgaccctatc tttacaactg ctgtggcaaa gcctattaaa
     7201 acaagtattt tatcaatatc agataagcta ctaggtttag agcaatttat caatattaat
     7261 gtcataccag gtaacagtca attaattaaa accttcataa tgtcacattg tcaagaacat
     7321 atttttagtt tgacagctga cgataatagt ctccttttga ttgcaaaatc agaagcagat
     7381 gctgatcaca ttcgtcaatc aatgattgca atgttggaga aaaaagatta gtagaggacc
     7441 ttatgctttt agaaatttct attaagaatt tcgctattat cgaagaaatt tcacttaatt
     7501 ttgaaacagg aatgactgtt ttaactggtg agactggtgc agggaaatct atcattattg
     7561 atgctatgaa tatgatgtta ggagcccgtg ctagtgttga agtgattcgc catggtgcta
     7621 acaaagcaga aattgaagga tttttctcta ttgaaaaaaa tcaatcatta gtccaattat
     7681 tggaagaaaa tggcattgaa ttagcagatg aattaattat tcgccgagaa atctttcaaa
     7741 atgggcgcag cgttagtcga attaatgggc aaatggtgaa tttgtcaaca ctaaaagctg
     7801 tggggcatta tttggtagac attcatggtc agcatgacca agaggaacta atgaaaccta
     7861 acatgcacat tctgatgtta gatgaatttg gtaatacaga atttaatgtc ataaaagagc
     7921 gttatcaaag tctttttgat gcttatcgtc agcttcgtaa acgtgtattg gataagcaaa
     7981 aaaatgaaca agagaataaa tcacgtattg aaatgctaga atttcaaata gcagaaattg
     8041 agtctgtagc ccttaaatca gatgaagacc aaacgctact caagcaacgt gataaattaa
     8101 tgaatcataa gaatattgca gatactttga caaatgcata tcttatgtta gataacgaag
     8161 agttttcaag tttatcgaat gttcgttctg caatgaatga ccttatggct ttagaagaat
     8221 ttgatcgaga atataaagat ctttccacca atctttcaga agcttactac gttattgaag
     8281 aagttactaa acgtttaggt gacgttatcg atgatttaga ttttgacgct ggtttactac
     8341 aagaaattga aaatagacta gatgttatta acacaataac gcgaaaatat ggcggtgatg
     8401 tgaatgatgt tttagactat tttgataaca ttactaaaga atatagtcta ttgacaggta
     8461 gtgaagagtc ctcagatgct ttagaaaaag aattaaagat tttagaacac gatttgattg
     8521 aatcagcaaa tcaattaagt ttagagcgtc ataagctagc taaacaattg gaaaatgaaa
     8581 tcaaacaaga attaacagag ctttatatgg agaaagctga tttccaagtt caatttacaa
     8641 aaggaaaatt taataaagaa gggaacgaaa tcgttgaatt ttatatttca acgaatcccg
     8701 gagagggctt taagccattg gtaaaagtgg cttcaggagg agagttatct cgattgatgt
     8761 tagctattaa atctgctttt tcacgtaagg aagataaaac ttccattgta tttgatgagg
     8821 tggatacagg agtttctggt cgcgtggccc aggctattgc tcaaaaaatt cacaagattg
     8881 gtagtcatgg acaagtttta gcaatttctc atttagctca ggttattgca atagcggatt
     8941 accaatattt tattgaaaaa atttccagtg actcttcaac agtttcaacg gtaaggcttt
     9001 taagctatga agaacgtgta gaagaaatcg ctaaaatgtt ggcaggaaac aatgtaactg
     9061 atactgcacg tactcaagct aaagaattac ttggtagtta gctttggtaa cgtctttaaa
     9121 aagctaacat tgttaggaga aattaatgtt gatacactta aattttgtta taataagggt
     9181 attgaaatat taaagtatag aataggatag gatatgagta aaatcaaaat tgtaacggat
     9241 tcttccatta ccattgaacc agaacttatt aaagagcttg acattacagt tgttcccttg
     9301 tcagttatga tagatggtac cttgtattca gacaatgact taaaagcaca aggggaattc
     9361 ttgaacctaa tgcgtggaag taaggaattg ccaaaaacaa gtcaaccacc tgtaggtgtt
     9421 tttgcagaaa tttatgaaaa attaatgaat gaaggtgcgg aacatatcat tgcaattcat
     9481 ttaacgcata cactatcagg aactattgaa gcatcacgcc agggagctaa tattgctggt
     9541 gcagatgtta cagttattga ttctactttt acagaccagt gtcaaaaatt ccaggttgta
     9601 gaagccgcga aattagctaa agagggagct gatttagata caattttggc tcgtgtggaa
     9661 gaagtacgcc agaagtcaga attatttatt ggcgtatcga cccttgaaaa tttagtaaaa
     9721 ggtggtcgta ttggacgtgt aacaggtctc ctaagttcat tgcttaacat aaaagtaatt
     9781 atggagctaa caaaccatga attagtaccg attgttaaag gtcgcggtct aaagaccttc
     9841 agcaaatggt tagataattt tgttgaaagt gcccaaacac gaaaaattgc tgaaattggc
     9901 atctcctact gtggtaaagc tgatatggct aataacttta aagaaaaact agctgttcta
     9961 ggtgctccta tatccgtatt agaaactggc tcaatcatcc aaactcatac tggtgaggat
    10021 gcatttgctg ttatggttcg ttatgaataa gaaaaaaata cttaccggac tttctttttt
    10081 tctggtaagt cttcttttat cttttgggat tttttccttg atcattccaa aatcaaatcc
    10141 taaattaaca aaaaaagact tcctaacaaa gaaagttatc ccacttaact atgttgctct
    10201 tggagattct ctgaccgaag gtgtggggga tacaacctct caaggtggtt ttgtcccact
    10261 gctatcagaa tcactccata atcgatactc ttaccaagtg acttctgtta attatggtgt
    10321 gtctgggaat actagtcaac aaattttaaa acgtatgacg acagatcctc aaatcgaaaa
    10381 agatttagag aaagctgatt tattgacgct aactgttggt ggtaatgatg tcttggctgt
    10441 tattcgtaaa gagctcagtc atttatcact aaattccttt gagaaaccag cagaagcata
    10501 taaggaacgt ttgaaagaaa ttcttgcaaa agcaagacaa gataatccta aattgcctat
    10561 ttatgtttta ggcatttata atccttttta cctaaacttt ccacaattaa ctaaaatgca
    10621 aaccgttatt gataattgga ataaagctac aaaagaagta gttgatgctt cagaaaatgt
    10681 ttattttgtc ccaattaatg accgccttta taagggaata aatggtaaag agggtattac
    10741 agagtcatca aatagtcagg caagtatcac taatgatgct ctctttactg gagaccattt
    10801 tcatcccaat aatattggct atcaaatcat gtctaacgcc gttatggaga aaataaatga
    10861 aacaagaaaa aactggccgt aatttaaatt tttggaaatg ggcatttctc ttattgttgg
    10921 caattaacct ttccttcaca gcagtgattg caagtcgttt aattcaagta cgtgagccta
    10981 atacaggaaa aatttcgact ggggtacaag ataaagtaaa agtaggtact tttacgacca
    11041 ataagtcgca actgaataag acaattgcac tttatttaaa acaatatcaa actaagaaga
    11101 tgaattataa gatttatgct gcttcatctt ctatactatt tgaaggctct tatcaattat
    11161 taggttatga agtgccttta tacatctatt ttgaacctta tcgtttaaca aatggcgctg
    11221 ttcaacttaa agtgacctca ttttcagtag gaactcttcc gcttccagaa aaagatgtgt
    11281 tacaatatat aaaatcatct tataaat
//
LOCUS       DATROD010000055        11071 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.055, whole genome shotgun sequence.
ACCESSION   DATROD010000055 DATROD010000000
VERSION     DATROD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11071
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.055"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..1045)
                     /locus_tag="VBC74_001302"
     CDS             complement(<1..1045)
                     /locus_tag="VBC74_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6942248.1"
                     /translation="MQKMTKLRRFRFPLRFYFTLMFVLTMLFSVLASLLLVAAIVFTF
                     FQGVLTTHVLQVSALAVVFLSLVIASISMWYGSYHLTKPILDISHIVSNVAGGDFEGH
                     IYRNSNRRKSYEYYNELDELSESINQMIVSLSHMDHMRKDFITNVSHELKTPIAAVAN
                     IVELLQDPELDEETQSELLGLVKTESLRLTRLCDTMLQMSRVDNQETIGELSSVRVDE
                     QIRQAMISLTERWQAKRINFQLDSKPYTVYSNSDLLMQVWINLLDNAIKYSEDIVDLS
                     VRMEETNNHYLRVIISDKGRGISQYDVQHIFDKFYQADQSHNQQGNGLGLAIVKRIIV
                     LCKGHISVSSQLEI"
     gene            complement(1029..1700)
                     /locus_tag="VBC74_001303"
     CDS             complement(1029..1700)
                     /locus_tag="VBC74_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6942249.1"
                     /translation="MIKILVVEDNIVQQKIITTKLTQEGYQFITASNGQEALNCLDTE
                     EVQLIITDIMMPMMDGYQLIQELRSAAYNVPIIVMTAKSQMEDMTKGFGLGADDYMVK
                     PVQLQELALRIKALLRRANIVAQHQLIIGNTCLNEDELSLKYFEQEIVFPQKEFRVLF
                     HLLSYPNRIFTRLELLDSIWGMDTDLDERVVDACINKIRRKVEHLPDFKIETVRGVGY
                     RAKND"
     gene            complement(1731..2417)
                     /locus_tag="VBC74_001304"
     CDS             complement(1731..2417)
                     /locus_tag="VBC74_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6942250.1"
                     /translation="MSAILELKHISKHYPDGDELLSILDNLDLSVSAGEFVAILGPSG
                     SGKSTLLSIAGLLLGADQGSLYVNHENVTDLSQRQRTQLRREALGFIFQSHQLLPYLT
                     IQEQLQQEARFAKHYDKKTSLEEINKLLSDLGIEQCAHKYPNQLSGGQKQRAAIARAF
                     INHPKVILADEPTASLDEERGRQVTELIRQEVKSHNTAAIMVTHDERVLDLVDTVYRL
                     KDGKLVKENF"
     gene            complement(2417..3505)
                     /locus_tag="VBC74_001305"
     CDS             complement(2417..3505)
                     /locus_tag="VBC74_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6942251.1"
                     /translation="MSHAWKELTFYKKKYLLIELLIIVMMFMVVFLSGLANGLGRAVS
                     AAIENNPAQTYILNEGAEQVITSSVLTTKDQTDLNSLNLKDSTTLNIQRSSLTRQGHE
                     KKIDISYFAIDKDSFMAPTLSEGKQLTSYKKAIILNDSLKAEGIKLGDKVIDKSSSIS
                     LTVVGFVHNSMYGHGPVAFIDKDIYTEINKKINPQYQFLPQALVMKNDKSISHLPTQL
                     EAVSKKDVIQHIPGYSAEQSTLNMILWVLVVASAGILGVFFYIITLQKRHEFSVMKAI
                     GTKMSEIALFQLSQVIILALFGIIVGDRLAIALSYVLPAQMPFVINWQNIILVSFVFL
                     VIAMISSALSIVKVAKIDPVEVINGGGE"
     gene            3687..4673
                     /locus_tag="VBC74_001306"
     CDS             3687..4673
                     /locus_tag="VBC74_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6942252.1"
                     /translation="MSTIRQVAEKAGVSTSTVSRYISQNGYVSQKASQKIEQAIRELH
                     YVPNFLAQSLKTKKNQLVGLLLPDISNPFFPRLARGVEEFLKEQGYRVMLGNTNNKSH
                     LEEEYLNVLLQSNAAGIITTHDFTKNHPEIDIPVVVVDRVNQETQYGVFSDNKEGGKL
                     AAQAIWTAGATNILLIRGPLDKADNLNQRFQGSQNYLLNKGARFAIEDSASFDFAEIQ
                     IEAKTLLDHHPDIDSIIAPSDIHAIAYLHEILNRGKRIPEDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYIMGQKAAELIFKITNQLPITNKRIKLPVHYVERETLRRKIDE"
     gene            4666..5577
                     /gene="rbsK"
                     /locus_tag="VBC74_001307"
     CDS             4666..5577
                     /gene="rbsK"
                     /locus_tag="VBC74_001307"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEO6942253.1"
                     /translation="MSNIVIIGSISMDLVMETNRIAKEGETVFGQRFSMVPGGKGANQ
                     AVAIGRLSQDRDNITILGAIGEDSFGPILLDNLNKNHVTTDFVGTIPSSSGVAQITLY
                     NNDNRIIYCPGANGKVDTKKWSQEWSIIKEADLVVLQNEIPHQANMKIANFCKEHSIK
                     LLYNPAPSRKTDIEMLDKVDYFTPNEHECQELFPNQKLEDILATYPEKLIVTLGTKGA
                     IYSDGKESHLIPALETKAVDTTGAGDTFNGAFGYAISKKFKIANALRFATLAAHLSVQ
                     KFGAQGGMPTIKEMEDNQHYEKDWYIK"
     gene            5552..5950
                     /gene="rbsD"
                     /locus_tag="VBC74_001308"
     CDS             5552..5950
                     /gene="rbsD"
                     /locus_tag="VBC74_001308"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEO6942254.1"
                     /translation="MKKTGILNSHLAKLADDLGHTDQVCIGDLGLPVPNGIPKIDLSL
                     TSGIPSFQEVLDIYLENILVEKVILAEEIKEANSDQLSRLLAKLDNSVNIEYVSHNHL
                     KQMTQDVKAVIRTGENTPYSNIILQSGVII"
     gene            5966..7444
                     /locus_tag="VBC74_001309"
     CDS             5966..7444
                     /locus_tag="VBC74_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEO6942255.1"
                     /translation="MKIDMRNISKSFGTNKVLEKIDLELQSGQIHALMGENGAGKSTL
                     MNILTGLFPASTGTIYIDGEERTFSNPQEAEEFGISFIHQEMNTWPEMTVLENLFLGR
                     EIKTTFGLLNQKLMRQKALEAFKRLGVTIPLDIPIGNLSVGQQQMIEIAKSLLNQLSI
                     LVMDEPTAALTDRETENLFRVIRGLKQEGVGVVYISHRMEEIFKITDFVTVMRDGVIV
                     DTKETSLTNSDELVKKMVGRKLEDYYPEKHSEIGPVAFEVSNLCGDNFEDVSFYVRKG
                     EILGFSGLMGAGRTEVMRTIFGIDKKKSGKVKIDNQEITITSPSQAIKQGIGFLTENR
                     KDEGLILDFNIKDNMTLPSTKDFSKHGFFDEKTSTTFVQQLINRLYIKSGRPDLEVGN
                     LSGGNQQKVVLAKWIGIAPKVLILDEPTRGVDVGAKREIYQLMNELADRGVPIVMVSS
                     DLPEILGVSDRIMVMHEGRISGELSRKEADQEKVMQLATGGK"
     gene            7446..8387
                     /gene="rbsC"
                     /locus_tag="VBC74_001310"
     CDS             7446..8387
                     /gene="rbsC"
                     /locus_tag="VBC74_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771495.1"
                     /note="functions to transport ribose at high affinity;
                     forms a complex with RbsA2C2B; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HEO6942256.1"
                     /translation="MKNMMKYISELTTLIALAGLMVIITLINPNFLTTNNLLNLLLQV
                     TANGFIAFGMTFVILTGGIDLSVGSILALSSALTAGLIGHGVPVGIAIVLSVALGGIL
                     GMLNGLFISYGKLAPFIVTLATMTIFRGATLVYSNGNPITAGLSDSFLFQFLGQGYIV
                     GIPFPVILMFLTFIILYILLHKTAFGKSVYALGGNEKAAYISGIKLNKVKIIIYTISG
                     IMASISGLIITSRLSSAQPTAGASYEMDAIAAVVLGGTSLSGGKGRIIGTLIGALIIG
                     VLNNGLNIIGVSAFWQQVVKGIVILMAVLLDRFKVAK"
     gene            8440..9408
                     /locus_tag="VBC74_001311"
     CDS             8440..9408
                     /locus_tag="VBC74_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEO6942257.1"
                     /translation="MKFGKKLGFLALLMSIVLILGACGKTGLGNSSGNSTKNVTKKSA
                     KDLKLGVSISTTNNPYFVAMKDGIDKYASNKKISIKVADAQDDAARQADDVQNFISQN
                     VDAILINPVDSKAIVTAIKSANNANIPVILMDRGSEGGKVLTTVASDNVAAGKMAADY
                     AVKKLGKKAKAFELSGVPGASATVDRGKGFHSVAKSKLDILSSQSANFDRAKALNTTQ
                     NMIQGHKDVQIIFAQNDEMALGAAQAVKSAGLQNVLIVGIDGQPDAHDAIKKGDISAT
                     IAQQPAKMGEIAIQAAIDHYKGKKVEKETISPIYLVTKDNVEKYNW"
     gene            complement(9448..10902)
                     /locus_tag="VBC74_001312"
     CDS             complement(9448..10902)
                     /locus_tag="VBC74_001312"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEO6942258.1"
                     /translation="MANKIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGDFVIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPETHENYRQSERLELYQRYIDQLLAEGKA
                     YKSYVTEEELAAERERQELAGETPRYINEFIGMSETEKEAYIAEREAAGIIPTVRLAV
                     NESGIYKWTDMVKGDIEFEGSNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPQFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     SEAVFNFIALLGWNPGGEEEIFSREQLINLFDENRLSKSPAAFDQKKMDWMSNDYLKN
                     ADFESVFALCKPFLEEAGRLTDKAEKLVELYKPQLKSADEIVPLTDLFFADFPELTEA
                     EKEVMAAETVPTVLSAFKEKLVSLSDEEFTRDTIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIYLLGKEKSVQHIDNMLAKL"
BASE COUNT         3569 a         2077 c         1932 g         3493 t
ORIGIN      
        1 caatttccaa ctgactggag acactaatat gtcccttaca aagaacaatt atacgtttaa
       61 ctattgctag tcctaatcca tttccttgtt gattatgcga ctgatccgct tggtaaaatt
      121 tatcaaatat atgctgaaca tcatattgag aaattcccct acctttatca gaaataataa
      181 ctctcagata atgattatta gtttcttcca ttcgtacact taagtctaca atatcctcgg
      241 aatattttat tgcattatct aaaaggttta tccaaacttg catcagcaag tcagagtttg
      301 agtaaacagt atatggtttt gaatccagtt gaaaatttat ccttttagct tgccaacgtt
      361 ctgttaaact aatcattgct tgacgaattt gttcatctac acgaacagag gatagctccc
      421 caatagtctc ctgattatcc acgcgcgaca tttgcaacat cgtatcacat aatcttgtta
      481 accttaggct ctctgtttta acaagaccta ataactcact ttgtgtttct tcatctaatt
      541 ctggatcttg taatagttct acaatattag ctactgcagc aatcggagtc tttagctcat
      601 gagaaacatt agtgataaag tcttttcgca tatgatccat atgagataaa gaaacaatca
      661 tctgattaat actttcagat agctcatcca attcattata atactcatag gatttacgac
      721 gattactgtt tcgataaata tgcccttcaa agtcaccgcc tgcaacattg gacacaatat
      781 ggctaatatc taaaattggt tttgttaaat gataagaacc gtaccacata ctaatactag
      841 ctatcacaag gcttaaaaaa acaacagcga gagcgctaac ttgtaataca tgagtagtta
      901 agactccttg aaaaaaagta aaaactattg ctgcaactag taaaagagaa gctaggactg
      961 agaaaagcat cgttaacaca aacattaatg tgaaataaaa tcttaacgga aaccgaaatc
     1021 gccttagctt agtcattttt tgcacgatac cctacccctc tcaccgtttc aatcttaaaa
     1081 tctggtaagt gttctacttt tcgtctaatt ttattgatgc aagcatctac cacccgctca
     1141 tctaaatcag tatccattcc ccaaatacta tccaatagtt ctaaacgtgt gaaaatccta
     1201 ttaggatagc taagtaagtg gaaaagaacc ctaaattctt tctgaggaaa tacaatttct
     1261 tgctcaaaat atttcaagct taattcatct tcattaagac aagtattccc aataattagc
     1321 tgatgttgag ctacaatatt agctctcctc aagagtgcct taatacgtaa agctaattct
     1381 tgtaactgca caggcttaac catataatca tctgcaccaa ggccaaaccc ttttgtcata
     1441 tcttccattt gtgattttgc tgtcataaca atgattggga cattgtaagc agctgacctg
     1501 agttcttgaa ttaattgata accatccatc atgggcatca tgatatcagt tataatcaat
     1561 tgtacttctt ctgtatctag acaatttaga gcttcttgtc catttgaagc agtgataaat
     1621 tggtagccct cttgtgttaa ttttgtggta atgatttttt gttgaacaat gttatcctct
     1681 acaactaata ttttaatcat taaatcacct ctagcttatt gtaacacaag ttagaagttt
     1741 tctttaacta atttcccgtc ctttaaacga taaacggtat ctactaaatc tagaacgcgc
     1801 tcatcgtgtg tcaccataat agctgcagta ttatgtgatt taacttcctg acgaataagt
     1861 tctgtaactt gacgcccacg ttcttcatca aggctagctg ttggctcatc tgccaagata
     1921 accttaggat gattaataaa tgctctagca attgctgcgc gttgcttttg tcctcccgat
     1981 aattggttag ggtatttatg tgcacactgc tctattccta aatctgataa taatttatta
     2041 atttcttcca agcttgtttt cttatcatag tgttttgcaa atcgagcctc ttgttgcaac
     2101 tgttcttgga ttgttaaata aggaagcagt tgatgacttt gaaatataaa acctagtgct
     2161 tcacggcgca attgtgttct ttgtctttga gacaaatccg taacgttctc atgattgaca
     2221 tacaaactac cttgatctgc accaagaagt aaccctgcaa tggataaaag tgtgctttta
     2281 cctgaacctg agggtcccag tatagctaca aactcacctg cagaaactga taaatctaaa
     2341 ttatctaata ttgataaaag ctcatctcca tctggatagt gttttgagat gtgttttaat
     2401 tctaagattg ctgacattat tctcctcccc cattaataac ttcgactggg tcgattttag
     2461 caacttttac aattgacaaa gcactactaa tcattgcaat gactaaaaat acaaacgata
     2521 ctagaataat attttgccaa ttaataacaa atggcatttg agctggtaat acataagaga
     2581 gagcaatcgc caatcgatct ccaacaatga taccaaacag tgccaatatg atgacttgac
     2641 tcaattgaaa tagggcaatc tctgacattt ttgtcccaat agctttcatg acgctaaatt
     2701 catgacgttt ttgaagtgtg atgatgtaga aaaagacacc taaaatacca gctgaagcta
     2761 cgacaaggac ccataaaatc atattcaaag tggattgttc agcactataa ccaggaatat
     2821 gttgaatgac atctttttta ctaacagctt ctaattgagt tggtaaatga gatatacttt
     2881 tatcattttt cataactaat gcctgaggta agaactggta ttgaggatta atctttttat
     2941 taatttcggt ataaatgtct ttatcaataa atgccacagg tccgtgacca tacatactat
     3001 tatggacaaa tccaacgact gttagtgata tagatgatga cttatcaatc actttatcac
     3061 ctaactttat cccttcagct tttaaactat catttaaaat aatagctttt ttgtaacttg
     3121 tcagttgttt tccctcagaa agtgtaggtg ccataaaaga atctttatca atagcaaaat
     3181 aagaaatatc aattttcttt tcatgtcctt gtcgagttaa actcgaacgt tgaatattta
     3241 aagtggtact atcttttaaa ttcaaactgt ttaaatctgt ctgatcttta gttgtcaata
     3301 cagatgatgt aatcacttgt tcagcacctt cattcaaaat ataggtttga gctgggttat
     3361 tttcaatagc agcactaaca gctctaccta aaccgtttgc taaacctgat aaaaaaacta
     3421 ccatgaacat cataacaatg attagcaatt caatcaggag gtattttttc ttataaaagg
     3481 ttaattcttt ccaagcatgt gacatggttt ttctcctttg atcttttgat gtgaacatag
     3541 tatcacagct ttatgacttg aatatgacct ctaaaaataa tgatatttta tcattaaatt
     3601 atttaaaaaa tagcttgaca attttttagt aagcgattac aattctaatt atgaaaccgt
     3661 tcccacaata aaagaaagga gtttctatga gtacgattag acaagtcgcc gagaaagctg
     3721 gcgtatcaac atctactgtc tcaaggtata tatcccaaaa tggttacgtt agtcaaaaag
     3781 caagtcaaaa aatcgagcaa gctatccgcg aactccatta tgttccaaat ttccttgcac
     3841 aatctctgaa aaccaaaaaa aatcaacttg ttggattact acttcccgat atttcaaacc
     3901 ctttctttcc aagacttgct agaggagttg aagaatttct aaaagaacag ggttatcgtg
     3961 tcatgcttgg caacacaaat aacaagagcc atttagaaga agagtactta aatgtgctat
     4021 tacaaagtaa tgctgcaggg attattacaa cacacgattt caccaaaaat catcctgaaa
     4081 tagatattcc agttgtagtt gttgaccgcg ttaatcaaga aacacagtac ggcgttttct
     4141 ctgataataa agagggagga aaattagcag ctcaagctat ttggacagca ggtgctacca
     4201 atatattact cattcgtggt ccgctagata aagctgataa ccttaatcaa aggtttcaag
     4261 gaagccaaaa ttacctatta aacaaagggg ctcgttttgc tatcgaagat agtgccagtt
     4321 ttgattttgc agagattcaa attgaagcga aaactttact tgaccaccat ccagatattg
     4381 atagcattat cgctccatct gatatacatg ctattgccta cctacacgag attcttaatc
     4441 gagggaagcg aatccctgaa gatgtacaaa ttattggtta tgatgatatt ttaatgagcc
     4501 aatttatcta cccttcgttg tcaactattc accaatcatc atatattatg ggacaaaaag
     4561 cagctgaatt aattttcaaa attactaatc aattacctat taccaataag cgtattaaat
     4621 tacccgttca ttatgtcgaa cgtgaaaccc taaggaggaa aatagatgag taacattgtc
     4681 attatcggta gtatttccat ggacttagtt atggaaacca atcgtatcgc caaagaggga
     4741 gaaaccgtat ttggccaacg tttttcaatg gttccaggag gtaaaggtgc taatcaggct
     4801 gtcgctattg ggcgtcttag ccaagatcga gataatatta ctattcttgg tgccattggc
     4861 gaggattctt ttggtcccat tcttttggat aatttaaaca aaaaccatgt tactacggat
     4921 tttgtgggaa cgataccatc ttcttcaggt gttgcccaaa tcactcttta taataatgat
     4981 aatcgaatca tttactgtcc tggagccaac ggaaaagttg atactaaaaa gtggtctcag
     5041 gagtggtcaa taattaaaga agctgattta gttgtattac aaaatgaaat tcctcatcaa
     5101 gcaaatatga aaattgctaa tttttgtaaa gaacatagta ttaaattact ctataatcct
     5161 gcaccatcta gaaagactga tattgagatg ctcgataagg tcgattattt cacaccaaat
     5221 gaacacgaat gtcaagagct gttcccaaac caaaaactag aggatatatt agccacatat
     5281 cctgaaaaac taattgtaac actcggcaca aaaggagcca tctactctga tggaaaagaa
     5341 agccatttaa ttcctgcctt agaaactaaa gctgttgata caactggtgc aggtgatact
     5401 tttaacggtg cattcggtta tgctatctcg aaaaaattta agattgctaa tgccttacgt
     5461 tttgcaactc tagctgctca cttatccgtg caaaaatttg gtgcacaggg tggcatgcct
     5521 acaattaaag aaatggagga caaccaacat tatgaaaaag actggtatat taaatagtca
     5581 tctcgctaaa ttagcagatg accttgggca tactgatcaa gtttgtattg gagatctggg
     5641 tctacctgtt ccaaatggca ttccaaagat tgatttgtct ttgacctctg gaatacctag
     5701 tttccaagag gtacttgata tctatttaga gaatatctta gttgaaaaag tcattttagc
     5761 agaagaaatc aaggaagcaa attcggatca attatcaagg ttattagcta aacttgataa
     5821 ctctgttaac atagagtacg tcagtcacaa tcatttaaaa caaatgaccc aagatgtcaa
     5881 agcagttatt cgtacaggtg aaaatacacc gtattcaaat attattctac aatctggtgt
     5941 tatcatctag aaaggagttt agtccatgaa aattgacatg aggaatatct ccaaatcctt
     6001 cggtactaat aaagttctag agaagattga tttggaatta caatctggtc aaatacacgc
     6061 acttatgggt gaaaatggtg cgggtaaatc aaccttaatg aacattctaa caggactttt
     6121 tccagcaagt acaggaacta tttatattga tggcgaggaa cgaacttttt caaaccctca
     6181 agaagcagag gaatttggta ttagttttat tcaccaagag atgaatactt ggccggaaat
     6241 gactgtcttg gaaaatctct ttcttggacg tgaaatcaag acaacttttg gcttactcaa
     6301 tcaaaaatta atgagacaaa aagcacttga agcttttaaa agattaggtg tgactattcc
     6361 cctagacatc ccgattggta acttgtctgt tggacaacaa caaatgattg aaatcgccaa
     6421 aagtctccta aaccaactct ctattttggt aatggatgaa ccgactgctg ctttaacaga
     6481 tcgtgaaact gaaaatcttt tccgagttat ccgaggttta aaacaagaag gagtgggcgt
     6541 tgtctatatc tcccatcgaa tggaggaaat ttttaaaatc actgacttcg taaccgtcat
     6601 gagagatggt gtcattgtag atacaaaaga aacatcactc acaaattctg acgaattggt
     6661 taaaaaaatg gttggccgaa aattggaaga ttactatcct gaaaaacatt ctgaaattgg
     6721 gcctgttgct tttgaagtta gtaacctctg tggagataat ttcgaggacg tttcctttta
     6781 tgtccgtaaa ggcgagattc ttggattttc tggattaatg ggagctgggc gtactgaagt
     6841 tatgcgaact atctttggaa ttgataaaaa gaagtctggt aaggtgaaaa ttgataacca
     6901 agaaatcact attactagcc caagccaagc tatcaagcag ggcattggtt ttttgaccga
     6961 aaatagaaaa gatgaaggtc tcatcctaga cttcaatatc aaggacaata tgactctccc
     7021 tagcactaaa gattttagca aacatggttt ctttgatgaa aaaacaagca caacatttgt
     7081 gcaacaattg attaatcgcc tctacattaa atcagggaga ccagatttag aggttggaaa
     7141 tctttctgga ggtaaccaac aaaaggtcgt tctcgctaaa tggattggta ttgctcctaa
     7201 agtcttaatt cttgatgagc caacacgtgg tgttgatgtc ggagctaaac gagagattta
     7261 ccaattaatg aatgaactag cagacagagg tgttccaatt gtcatggtat catcagactt
     7321 acctgaaatt ctaggtgtta gtgaccgcat catggtaatg cacgaaggtc gaatcagtgg
     7381 agaactcagt cgaaaagaag ctgaccaaga aaaagttatg cagctagcca caggaggtaa
     7441 gtaaagtgaa aaatatgatg aaatatatat cagagttaac aaccctgatt gcattagctg
     7501 gtttaatggt tattatcaca ctcattaatc ctaatttcct cacaaccaat aatctactca
     7561 atttattatt acaagtaact gcaaatggtt ttatcgcttt cgggatgacc ttcgttattt
     7621 tgactggcgg tattgattta tcagtcgggt ccatattagc cctatctagt gcattaacag
     7681 ctggattaat cggccacgga gtacccgttg gtattgctat cgttttatct gttgccttag
     7741 gtggcatctt gggaatgcta aacggactct tcatttctta cggaaaatta gcaccattta
     7801 tcgttacact ggcaacaatg actatcttta gaggagcaac gctagtttat tcaaacggca
     7861 acccaattac cgctggatta tccgatagtt ttctattcca atttttggga caaggctata
     7921 ttgttggtat cccattccca gtcattctca tgtttcttac ttttattatt ttgtatattt
     7981 tgttacataa aacagccttc gggaaatctg tttacgcttt aggaggtaat gaaaaagcag
     8041 cgtatatctc gggtattaag ttaaacaaag ttaaaattat catctacact atctcaggta
     8101 tcatggcttc catttctggg ttaattatca catcacgcct tagctcagca caaccaacag
     8161 ctggagcaag ttatgagatg gatgctattg ccgccgttgt cttaggtgga acgtctttat
     8221 ctggtgggaa aggacgtatc attggaacat tgattggtgc tctcattatt ggtgtcctga
     8281 acaatggatt aaacatcatt ggtgtctctg cattttggca acaagttgtg aaaggtatcg
     8341 ttattttaat ggctgtttta cttgatcgct ttaaagtagc aaaatagtct ttactctaat
     8401 ttctataagt tcattatatt ttatctaaag gagtttcaaa tgaaatttgg aaaaaaactt
     8461 ggctttctag ccttattgat gtctattgtc ctcattctcg gtgcatgtgg taaaactgga
     8521 cttggaaatt cttctgggaa ttctacaaaa aatgtaacca aaaaatctgc taaagatttg
     8581 aaactaggtg tatctatttc aacaactaac aacccctact ttgttgcgat gaaagacggt
     8641 attgacaaat atgcgagcaa taaaaaaata agcattaagg tagctgatgc tcaagatgac
     8701 gccgcacgtc aagcagatga tgttcaaaac tttattagtc aaaacgtaga tgctattttg
     8761 attaatcccg ttgactcaaa agctattgta acagctatta agtctgcaaa caacgctaat
     8821 attcctgtta ttctaatgga ccgtggtagt gagggaggca aagtattaac tactgtcgct
     8881 tcagataacg ttgctgctgg taagatggct gcggattatg ccgttaaaaa actaggcaaa
     8941 aaagcaaaag cctttgaatt atctggagta ccaggtgcct ctgctactgt agaccgtggt
     9001 aaaggatttc attcagttgc aaaatctaaa cttgacattc tttcaagcca atctgccaat
     9061 tttgaccgcg ccaaagcttt gaatactaca caaaatatga ttcaaggaca taaagatgtc
     9121 caaattattt tcgcacaaaa tgatgaaatg gcacttgggg ctgcacaggc tgtcaaatct
     9181 gctggcttgc aaaatgttct tatcgtagga attgatggtc agccagatgc ccatgatgcc
     9241 attaaaaaag gtgatatttc agccactatt gcacaacaac cagctaagat gggagaaata
     9301 gctattcaag cagcaattga tcattataaa ggcaaaaaag tagagaaaga aacgatttct
     9361 ccaatttacc ttgttaccaa agacaatgtt gaaaaataca attggtaaaa gtacacaaaa
     9421 agtcgtgaaa attcacgact ttttatatta taattttgct aacatattat cgatatgttg
     9481 aactgatttt tctttaccta ataaataaat agtatctggt aactctggtc catgcatttc
     9541 accagaaacc gcaatacgaa taggcataaa taggttctta cctttgatgc ctgtttcttt
     9601 ttgaacagcc ttaatttgag ggaaaatagt atctctagtg aattcttcgt cagaaagcga
     9661 tactaacttt tccttaaaag cagataagac tgttgggact gtttctgctg ccattacttc
     9721 tttttcggct tctgttaatt ctggaaaatc tgcaaagaaa aggtctgtta atggtacaat
     9781 ctcatcagcc gattttaatt gaggtttata aagctcaact aatttttcag ctttgtctgt
     9841 aagtcgacca gcttcttcta agaatggttt gcaaagtgca aaaacactct caaaatcagc
     9901 attttttagg taatcattac tcatccaatc catcttcttc tgatcgaatg ctgctggtga
     9961 tttgctaaga cgattttcat caaatagatt aattaattgc tcgcgtgaga agatttcttc
    10021 ttctccacca ggattccaac caagaagggc aatgaaatta aagacagctt ccgacatata
    10081 acctttttta cgatagtctt cgataaattg aagagtattt gtgtcgcgtt ttgataattt
    10141 cttaccagtc tctgagttta taatcaatgt catgtgacca aattgtggcg cttcccaacc
    10201 gagtgcttca taaaccatta attgttttgg cgtatttgca atatggtcat cgccacgaat
    10261 aacatgcgaa atttgcatgt catgatcatc aatgactact gcgaaattgt aagttggata
    10321 accatctttc ttctgaataa cccagtctcc gccgatatta gagccttcaa actcaatgtc
    10381 accttttacc atatcagtcc acttataaat cccagattca ttaacagcaa ggcgtactgt
    10441 cggaatgatg cctgctgctt cacgttctgc aatgtaagct tctttttcag tttctgacat
    10501 tccgataaat tcgttgatat agcgaggtgt ttcgcctgcc aattcttggc gttcacgctc
    10561 tgctgctaat tcttcctcag tcacatagga cttgtaggct ttaccttctg ctaacaactg
    10621 atcaatataa cgttggtaga gctcaaggcg ttctgattga cgataatttt catgtgtttc
    10681 agggctttca tcccagtcca tacctagcca acgtaagttc tccaattgag agcgttcacc
    10741 atcttcgacg tgacgttttc ggtcagtatc ttcaatacga ataacaaaat ccccaccatg
    10801 atggcgagca taaaggtagt taaagagcgc agtacgcgca tttccgatgt gtaacaatcc
    10861 tgttggactt ggtgcataac gcacacgaat cttattagcc aaaattctac tcctatattt
    10921 tctgatagta aactttttca gtttaactga attagtcaag ggggcaaaac atcattcaaa
    10981 ctcttacaga tgattgccaa tcaaccctat tataccacta aatcactaaa aatgataatc
    11041 agtaataata gtcacactaa ttttcgataa a
//
LOCUS       DATROD010000056        10969 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.056, whole genome shotgun sequence.
ACCESSION   DATROD010000056 DATROD010000000
VERSION     DATROD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10969
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.056"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..588
                     /locus_tag="VBC74_001313"
     CDS             <1..588
                     /locus_tag="VBC74_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEO6942259.1"
                     /translation="DDNILAEEDDVRSPIAQGNVWVLDPIDGTVNFIVQKDNFAVMLA
                     YYEEGVGQFGIIYDVMADILYSGGGHFDVYANDKKIVPFQECPLERCLLGVNSAMYAE
                     NDCGIAHLASETLGVRIYGGAGISMAKVMQGKLLAYFSYIQPWDYAAAKIMGETLGFT
                     LLTLDGEEPNYSTRQKVMFLPKSKLNLIQSYLTKK"
     gene            656..1966
                     /locus_tag="VBC74_001314"
     CDS             656..1966
                     /locus_tag="VBC74_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEO6942260.1"
                     /translation="MKLPNEFIEKYQTILKDEAEAFFDSFEQKPISAYRTNPLKEKQL
                     DFPNAIPSTPWGHYGKISGKSIEHTTGLVYSQEPAAQIVAQIAEPQEGMKVLDLAAAP
                     GGKTTHLLSYLNNTGLLVSNEISNKRSKILVENVERFGARNVIVTNESSQRLAKCFNS
                     FFDLIVFDGPCSGEGMFRKDPQAIQYWHKDYPTECAQLQRDILKEAIKMLAHGGILVY
                     STCTWSPEENEEVVNWLLQEYDYLELVDIPKLNGMVEGINVPQVARMYPHHFQGEGQF
                     VAKLRDTRSKEAQKIKPKAQKINKMQLQLWQQFAQDHLKIDLNGVLDVFGDQLYLLPN
                     GLPDLSKLKIARNGLHLGTFKKNRFEPSFALGMALSEHDLVQSIEIDIEQFEVYVSGN
                     VVKLDKTVPNGWYQILVKGNGLGFAKVTNNTLKNYYPKGLRFQT"
     gene            2145..3011
                     /locus_tag="VBC74_001315"
     CDS             2145..3011
                     /locus_tag="VBC74_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEO6942261.1"
                     /translation="MKMKKNIFLLTLLATVLLTLSGCANWIDKGQSITSVGSTALQPL
                     VEAAADEFGKTNLGKTINVQGGGSGTGLSQVQSGAVQIGNSDLFAEEKDGIDAKKLKD
                     HQVAVAGLAVIVNKKVNVKNLTTHQLRDIFAGKIKNWKEVGGQDLDISIINRAASSGS
                     RATFDNTIMGNVAPIQSQEQDSNGMVKSIVSQTPGAISYLAFAYVDKSVGTLKLNGFA
                     PTAKNVTTDNWKLWSYEHMYTKGNETGLTKEFLDYMKSDKVQSSIVQHMGYISINDMK
                     VVKDAEGKVTPK"
     gene            3057..3974
                     /gene="pstC"
                     /locus_tag="VBC74_001316"
     CDS             3057..3974
                     /gene="pstC"
                     /locus_tag="VBC74_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO6942262.1"
                     /translation="MKNQELAKKLTSPSKNSRLEKFGKGITFLSLALIVFIVAMILIF
                     VAQKGLSTFFVDGVKLTDFLFNTKWEPSAKSFGALPMIAGSFIVTILSAIIATPFAIG
                     AAVFMTEISPKYGSKILQPAVELLVGIPSVVYGFIGLQIIVPFVRSIFGGTGFGILSG
                     VCVLFVMILPTVTFMTVDSLRAVPRHYKEASLAMGATRWQTIWRVILNAARPGIFTAI
                     VFGMARAFGEALAIQMVVGNSAILPTSLTTPAATLTSVLTMGIGNTVMGTVQNNVLWS
                     LALVLLIMSLAFNTVIKLITREGKKNYER"
     gene            3964..4851
                     /gene="pstA"
                     /locus_tag="VBC74_001317"
     CDS             3964..4851
                     /gene="pstA"
                     /locus_tag="VBC74_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO6942263.1"
                     /translation="MNAKKADKLATTILYSIAAIIVTILASLLIFILVRGLPHVSWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSLGAGIYLSEYAKKGRLTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFQYGFSIISGALALTVFNLPQMTRSVEDSLQNVH
                     HTQREAGLALGISRWETVIYVVVPEALPSIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNVLSVTSPISIFRQAETLAVHIWKVNSEGTIPDATQVSAGSAAVLLVVILIF
                     NLSARSIGKKLHSKLTSSN"
     gene            4863..5666
                     /gene="pstB"
                     /locus_tag="VBC74_001318"
     CDS             4863..5666
                     /gene="pstB"
                     /locus_tag="VBC74_001318"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO6942264.1"
                     /translation="MAEYNWDERHIITFPEENSALTTKDLHVYYGEKEAIKGIDMQFE
                     KNKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGQIMYEGIDVNAQDINVYEMRKH
                     IGMVFQRPNPFAKSIYKNITFAYERAGVKDKKFLDEVVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAIAVKPEILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGDLIEYDKTNNIFQNAKCQSTSDYVSGRFG"
     gene            5678..6436
                     /gene="pstB"
                     /locus_tag="VBC74_001319"
     CDS             5678..6436
                     /gene="pstB"
                     /locus_tag="VBC74_001319"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO6942265.1"
                     /translation="MTQPILQVSDLSVYYNKKKALKEVSMDFYPNEITALIGPSGSGK
                     STLLRAINRMGDLNPEVTLTGAVMYNGHNVYSPRTDTVELRKEIGMVFQQPNPFPMSV
                     FENVVYGLRLKGIKDKATLDEAVETSLKGASIWDEVKDRLHDSALGLSGGQQQRVCIA
                     RTLATKPKIILLDEPTSALDPISAGKIEETLHGLKDQYTMLLVTRSMQQASRISDRTG
                     FFLDGNLIEYGNTKEMFMNPKHKETEDYITGKFG"
     gene            6470..7123
                     /gene="phoU"
                     /locus_tag="VBC74_001320"
     CDS             6470..7123
                     /gene="phoU"
                     /locus_tag="VBC74_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO6942266.1"
                     /translation="MLRSKFDEELDKLHNQFYAMGIEAIGQIKKTVRAFVSHDRELAK
                     EVIEDDVTLNNFETKLEKKSLEIIALQQPVSQDLRTVITVLKATSDVERMGDHAAAVA
                     KATIRMKGEERIPAVELEINNMGKAVKNMLEEALTAYINGDDEKAYEVAAMDEIVDDY
                     FRDIQKMVVETIQKHPDVAFAAKEYFQVLMHLERIGDYGKNICEWIVYLKTGKIIEL"
     gene            7269..9818
                     /locus_tag="VBC74_001321"
     CDS             7269..9818
                     /locus_tag="VBC74_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEO6942267.1"
                     /translation="MKTVEHFVTKFVPENYNLFLDINRQTKTFSGNVAVSGEALDNNI
                     SFHQKGLTIKSVLLDNQPLDFQLDEDNEAMHIQLHETGSMVLVFEFSGHITDNMTGMY
                     PSYYTVNGIKKEVISTQFESHFAREVFPSIDEPEAKATFDLSLKFDQKEGEIALSNMP
                     EINAEQRQETGLWTFDTTPKMSSYLLAFALGELHGKTTHTKNGTLVGSYATKAHQLNE
                     LDFSLDIVVRVIEFYEDYFGVRYPIPQSLHVALPDFSAGAMENWGLVTYREVYLLVDE
                     NSSVSSRQQVALVVAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIDYIEPKLN
                     IFEDFQTGGLPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRW
                     LGDTDFAAGLKIYFEKHQYQNTIGRDLWNALSQTSGKDVAAFMDSWLEQPGYPVMAAK
                     IEEDELILTQKQFFIGEHEDKSRLWQIPLNSNWEGIPEILTEETVVIPNFSQLAEKNK
                     ENGALRFNTENTAHYITNYQGQLLEHIISDLPLMDNISKLQIVQERHLLAESGMISYS
                     SLIPLVSLLSQETSYLVNSAIKSVIDGLSLFVQEDSQDEFDFKEFVNKLSAFNFNRLG
                     FEKREGEGDDSEMVRHLSLSLALYSDNEHAIEEAHHIFKAHENNIAAIPAAIRLLVLT
                     NEMKHFESKELSHLLLETYSTTTDGNFKRQLASALSHTTDSKTLKKLLSDWKNKDIVK
                     PQDLAMSWYATFLKNSFTQESVWEWAQENWEWIKATLGGDMSFDKFVIYPSSSFKTEE
                     RLEQYKNFFEPQLSDMAISRNISMGIKEISARVLLITKQKEEVINTIKKYI"
     gene            9980..10660
                     /locus_tag="VBC74_001322"
     CDS             9980..10660
                     /locus_tag="VBC74_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6942268.1"
                     /translation="MIKILLIEDDLSLSNSVFDFLDDFADVMQIFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESIDDKGQGFDLGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNDNSLIYGDIRVDMSTNSTFVNQTEVELLGKEFDLLVY
                     FLQNQNVILPKSQIFDRIWGFDSDTTISVVEVYVSKVRKKLKGTLFSENLQTLRSVGY
                     ILKHVETD"
     gene            10644..>10969
                     /locus_tag="VBC74_001323"
     CDS             10644..>10969
                     /locus_tag="VBC74_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6942269.1"
                     /translation="MSKPIKISKFKKNVSDSHFIHFFTVFSGIFLVMTVIILQVMRYG
                     VYSSVDSSLKYISTHPKNYINMVMSRTAAYLDNSNIASVKLKPGGQTVANTDIILFTS
                     EEEVIN"
BASE COUNT         3628 a         1907 c         2041 g         3393 t
ORIGIN      
        1 gatgataata tacttgccga agaagatgat gtgcgctcac ccattgcaca aggtaatgtt
       61 tgggttttag accctattga tggaactgtt aattttattg ttcaaaaaga taactttgct
      121 gttatgttag cttattacga agaaggcgtt ggacaatttg gtatcatcta cgacgttatg
      181 gctgatattc tttacagcgg gggaggacat tttgatgtct atgctaacga taaaaaaatt
      241 gttccttttc aagagtgtcc tttagaacgt tgcttacttg gagtaaattc tgctatgtat
      301 gcagaaaatg attgcggtat tgcccattta gcttctgaaa ccttaggtgt tcgtatctat
      361 ggtggtgctg gaattagcat ggcaaaagtc atgcagggca aattattagc ttatttttcg
      421 tatattcaac cttgggatta tgctgcagca aaaatcatgg gggagacatt aggatttacc
      481 ttattaacgc ttgacgggga agaacccaac tattcaacaa gacaaaaagt aatgttccta
      541 ccaaagtcga aattaaatct tatccaatcc taccttacca aaaaataaac ttaaaacctt
      601 ctaattaaat agaaggtttt tttgcacgcc cgtgattatg tagtataata gaaacatgaa
      661 attaccgaat gaatttattg aaaaatatca gactatactt aaagatgaag cagaagcttt
      721 ttttgacagt tttgaacaaa aacccatatc ggcatatcgc accaatcctc tcaaagaaaa
      781 gcaacttgat tttccaaatg ccatcccaag taccccttgg gggcattatg ggaaaatatc
      841 aggaaaatca atagaacaca caactggcct tgtttattct caagaacctg ctgctcagat
      901 agtggctcag attgctgaac cacaagaagg aatgaaagtg ctagatttag cagctgcacc
      961 tggtggaaaa acaacacacc ttctatcata tttaaacaat actggtctat tagttagtaa
     1021 tgaaatttca aataaacgta gtaaaatttt agtcgaaaat gtcgaacgtt ttggtgctag
     1081 aaatgttatc gtgactaatg aaagctccca acgactagcc aaatgtttta attctttttt
     1141 tgatcttatt gtttttgacg gtccgtgttc tggtgaggga atgttccgca aagatcctca
     1201 agcaatacaa tattggcaca aagattaccc cactgaatgt gcacagttac aaagagatat
     1261 tttaaaagaa gcaatcaaaa tgctagctca tggtggtata cttgtatact ctacttgtac
     1321 atggtcacca gaagagaacg aagaagtggt taattggctc cttcaagagt atgattactt
     1381 agaacttgtt gatattccta aactaaatgg gatggttgaa gggattaatg taccacaagt
     1441 tgcaaggatg taccctcatc attttcaagg tgaaggacaa ttcgttgcaa aactgagaga
     1501 tactagatct aaggaagcac agaaaattaa gccaaaagca cagaaaataa ataaaatgca
     1561 gttacaattg tggcaacaat ttgcacaaga ccatttaaag atagacttga acggtgtttt
     1621 agatgttttc ggcgaccaac tctatcttct acctaatggt ctccctgatt tgtctaaatt
     1681 aaaaatagca cgcaatggtc tccatctagg cactttcaag aagaatcgtt ttgagccatc
     1741 gtttgcttta ggaatggcac ttagcgaaca tgacctagta cagtctattg aaattgatat
     1801 agaacagttt gaggtgtacg tatctggaaa tgtagtcaaa ctagacaaga ctgttccaaa
     1861 cggttggtat caaattcttg taaaaggcaa tggattaggt tttgcaaaag tgacaaataa
     1921 tactcttaaa aattattacc ctaaaggact acgatttcag acataactgt tattgaatac
     1981 ttttaataaa aaaggaaaaa gaaatacttt tcaaacccta gaaacttatg gtactatggg
     2041 acagtgatgt gtgtttttac tatttgtaaa tcattaaaca ctcaatgatt ttagtaatat
     2101 atatcaacta ctaaatatca aaatttattc aaaagaatag gaaaatgaaa atgaaaaaaa
     2161 acattttttt gttaaccttg ctagccaccg tcttattgac gctgtctggt tgtgctaatt
     2221 ggattgataa aggtcaatca atcaccagcg ttggttcaac cgccttacag ccacttgtag
     2281 aagctgcagc agatgagttt ggtaagacaa acctcggtaa gactatcaat gtccaaggag
     2341 gaggctcagg aacaggctta tcacaagtac agtctggagc tgttcaaatt ggtaatagcg
     2401 acttatttgc agaagaaaaa gatggtattg atgctaagaa attgaaagac catcaagtcg
     2461 cagtagctgg attagctgtt attgttaata aaaaagttaa tgttaaaaat ctaaccaccc
     2521 accaacttcg tgatattttt gcaggaaaaa tcaagaattg gaaagaagtg ggaggacaag
     2581 atttagatat ttccatcatc aatcgagcag caagttctgg atcccgtgca accttcgata
     2641 atactattat gggaaatgtt gctcctattc aaagccaaga acaagattca aatggtatgg
     2701 tgaaatcaat tgtttctcaa acacctggtg cgatttcata cctcgctttt gcatacgttg
     2761 ataaatctgt cggaacatta aaactaaatg gttttgcgcc aacagctaaa aatgttacta
     2821 ccgacaactg gaaactttgg tcatatgaac atatgtatac aaaaggtaat gaaacaggtc
     2881 taacaaaaga attcttagac tacatgaaaa gtgataaagt tcaaagtagt atcgtacaac
     2941 atatgggata tatctctatt aacgacatga aagttgttaa agatgctgaa ggtaaagtaa
     3001 ccccaaaata aaacttaatc acatctagat aaagcatctt gatcctagga ggaattatga
     3061 aaaatcaaga attagctaaa aaattaacat ctccttcaaa aaactctcga ttagaaaaat
     3121 ttggtaaagg aataactttt ctttcgcttg ccctaatcgt cttcattgtt gcgatgattt
     3181 tgatttttgt tgctcaaaaa ggactatcta cattttttgt tgatggcgtt aaattaacag
     3241 acttcttatt taatacaaaa tgggaaccaa gtgctaagtc attcggtgct cttccaatga
     3301 tagctggatc atttattgtt actattctat cagctatcat tgctacacca tttgctatcg
     3361 gtgctgccgt atttatgact gaaatctcac ctaaatatgg ttctaaaatt ttacaacctg
     3421 cagttgaatt actagttggg attccttctg ttgtttacgg ttttattggt ttacagatta
     3481 ttgttccctt cgttcgctct atttttgggg gaacaggttt tggcatttta tctggggtct
     3541 gtgtcctctt cgtcatgatt ctaccaactg ttactttcat gacagtagat agccttagag
     3601 ctgtgccacg tcactataaa gaagcaagtt tagccatggg ggcaactcgt tggcaaacaa
     3661 tttggcgtgt tatattaaac gctgcacgtc caggaatttt cactgcaatt gtttttggta
     3721 tggcacgtgc atttggtgaa gctctagcaa ttcagatggt tgtcggaaac tctgcaattt
     3781 taccaacatc attgacaaca ccagcagcaa cattaacgtc tgttctaaca atgggtattg
     3841 gtaatactgt aatgggaact gtacaaaaca atgtcctctg gtcgctagcc ttagtacttt
     3901 taattatgag tttagcattt aatacagtta ttaagttaat tacaagggaa ggaaagaaaa
     3961 actatgaacg ctaaaaaggc tgacaaatta gcaactacaa tactctatag tattgcagct
     4021 atcatcgtta caatcctcgc ctctctttta atttttattc ttgttcgcgg attgccacac
     4081 gtatcttggt catttttaac tgggaaatca tcatcatatg aagcaggagg cggtattgga
     4141 atacaattat acaattcctt tttcctactt attgttactc tgattatttc tattcctcta
     4201 tctttaggag ctggaatata cctctcagaa tatgctaaaa aaggacgttt aacaaacttt
     4261 gttagaacat gtatcgaaat tttgtcatct cttccttctg ttgtagttgg tctgtttggt
     4321 tacctaatct tcgttgttca atttcaatat ggcttctcaa ttatctcagg tgccttagca
     4381 cttactgttt ttaacttacc ccaaatgaca cgtagtgttg aagatagcct tcaaaatgta
     4441 caccacacac agcgtgaagc aggattggcc ctaggtattt ctcgttggga aacggtcatt
     4501 tacgtcgttg tccctgaagc tctgccaagt attgtaactg gtgttgtgct agcttcagga
     4561 cgtattttcg gtgaggcagc agcacttatt tatacagctg gtcaatctgc tccagcactt
     4621 gattggtcaa attggaacgt ccttagtgtt acaagtccaa tctcaatttt ccgtcaagca
     4681 gaaactttag ctgttcatat ttggaaagtt aatagcgaag ggacaatccc tgatgcaaca
     4741 caagtatctg ccggtagcgc cgcagtctta ttggttgtta ttcttatttt caacctttca
     4801 gctcgctcga tcggtaagaa acttcattcg aaattaacct cgtctaatta aaaggagtag
     4861 taatggcaga atataattgg gacgaacgcc atatcattac cttccctgaa gaaaattcag
     4921 ctctcaccac aaaagattta cacgtttact acggtgagaa agaagctatc aagggaattg
     4981 atatgcaatt tgaaaaaaat aaaattacag ccttaatcgg cccatctgga tgtggaaaat
     5041 caacatatct tcgtagctta aatcgtatga acgatacgat tgatattgca cgcgtgactg
     5101 gacaaatcat gtatgaagga attgatgtta acgctcaaga tatcaatgtt tacgagatgc
     5161 gaaaacatat cggaatggtt ttccaacgtc caaacccgtt tgcaaaatca atttataaaa
     5221 acattacctt tgcatatgag cgtgctggtg taaaagataa aaaattctta gatgaagttg
     5281 tcgaaacctc actaaaacaa gctgctctat gggatcaagt aaaagatgac cttcataaat
     5341 cagcattcac tttatctggc ggtcaacagc agcgattatg tattgcacgt gctatcgcag
     5401 ttaaaccaga aattctctta atggatgaac ctgcttcagc actcgatccg attgcaacta
     5461 tgcaactaga agagacaatg tttgaactca agaaaaatta cactataatt atcgtaacac
     5521 ataatatgca acaagcagca cgtgctagtg attatactgc atttttctat cttggagact
     5581 taatcgaata cgataaaacg aataacattt tccaaaatgc taaatgtcaa tctactagcg
     5641 attatgtttc tggtcgattt ggatagtgag gtaaaagatg acacaaccta ttttacaagt
     5701 cagtgatttg tctgtttact ataataagaa aaaagcttta aaagaagtct caatggactt
     5761 ctatcctaat gaaattacag ccttaattgg tccgtctggt tctggaaagt caacactctt
     5821 acgagctatt aaccgtatgg gagacttaaa tcctgaagtc actctaacag gagcagtgat
     5881 gtataacgga cataatgttt atagcccgcg cactgatacc gtagaacttc gtaaagaaat
     5941 tgggatggtt tttcagcagc ctaatccatt tcctatgtca gtatttgaaa atgttgttta
     6001 tggtcttaga ctcaaaggaa taaaagacaa agctacgtta gatgaagccg tagagacttc
     6061 attgaagggc gcgtctatct gggacgaggt caaagataga ttacacgact ctgctcttgg
     6121 tttatctggt ggtcaacaac aacgtgtttg tattgccaga acccttgcta ccaagcctaa
     6181 aattatcctt ctagatgagc caacctctgc acttgaccca atttctgctg gtaaaattga
     6241 agagacttta catggtctaa aagatcaata taccatgtta cttgttacac gttctatgca
     6301 gcaagcctca cgtatttctg atcgtacagg tttcttctta gacggtaact taattgaata
     6361 tggcaataca aaagaaatgt ttatgaatcc taaacataaa gaaactgaag attacattac
     6421 aggtaaattc ggttaatacc atataataag atcagaaaga gaatacaata tgttaagatc
     6481 aaaatttgat gaagaattag ataaactgca taaccaattc tatgcaatgg gaattgaagc
     6541 tatcggacaa atcaaaaaaa cagttcgtgc ttttgtcagt catgatcgag agttagctaa
     6601 agaagtgata gaagatgatg taacgcttaa caactttgaa acgaagttag agaaaaaatc
     6661 attggaaatt attgcactcc agcagcctgt atcacaagac ttacgcaccg ttattaccgt
     6721 tttaaaagct actagtgatg ttgagcgtat gggagaccat gcagcagcag ttgcaaaagc
     6781 aaccatccgt atgaagggcg aggaaagaat cccagcagta gaattagaaa tcaataatat
     6841 gggaaaagca gttaaaaaca tgcttgaaga agccttaaca gcttatatca atggtgatga
     6901 tgaaaaagct tacgaagtag cagcaatgga tgaaatagtt gatgattact tccgtgatat
     6961 ccaaaaaatg gtagttgaaa caattcaaaa gcatcctgac gtagcatttg ccgccaaaga
     7021 atacttccaa gtattgatgc acttggagcg aattggagat tatggcaaaa atatctgtga
     7081 atggattgtt tatttaaaaa ctggaaaaat tatcgagtta tgatataatt attgcttgta
     7141 gagtggtaat tgtaagtata gttcgtgcga tagaaaagct gtaactatct tgttacagct
     7201 tttctatctt ttctagccca tacaggacta gtaatgtttc aaatgtttct gaattagtta
     7261 ggagttatat gaaaactgta gaacattttg ttaccaaatt tgtccctgaa aactataacc
     7321 ttttcttgga tattaatcgt caaactaaaa ccttttcagg taacgttgct gtgtctggcg
     7381 aagccttaga taacaatatc tcttttcacc aaaaagggtt aaccattaaa tctgtcttat
     7441 tagataatca accattagat ttccaactag atgaagacaa tgaagctatg catattcaat
     7501 tgcatgaaac tggtagcatg gtattagtct ttgaattcag cggtcacatt accgataata
     7561 tgacaggtat gtatccatct tactatacag ttaatggcat caaaaaagag gttatctcca
     7621 cacaatttga aagtcatttt gctcgtgaag tattcccaag tattgacgaa ccagaagcaa
     7681 aagcaacctt tgatttgagc ctaaaattcg atcaaaaaga aggagagatt gctctttcaa
     7741 atatgccgga aatcaatgct gaacaacgtc aagaaacagg tttatggaca tttgacacca
     7801 cacctaaaat gtcctcatat ttattagctt ttgccttggg ggaattgcat ggtaaaacaa
     7861 cacataccaa aaacggcact cttgttggct catatgccac aaaagcccat cagttaaatg
     7921 aattagattt ttctttagat atcgttgtgc gtgttattga gttttatgag gactactttg
     7981 gtgtacgtta tcctattcct cagtcactcc acgttgctct acctgatttt tctgctggag
     8041 ctatggaaaa ttggggatta gtaacatatc gtgaagtcta tcttttagta gatgagaact
     8101 ctagtgtttc cagccgtcag caagtagcat tagttgtagc acatgaaata gctcaccagt
     8161 ggtttggtaa cctcgttact atgaaatggt gggatgattt atggttaaat gaatcatttg
     8221 ctaatatgat ggaatatgta tcaattgatt atattgaacc aaaacttaat atatttgaag
     8281 actttcaaac aggtggtcta ccattagctc ttaagcgtga tgccacagat ggcgtccaat
     8341 ctgttcacgt tgaggtcaat catcctgatg aaattaacac actttttgat ccagctatcg
     8401 tttatgcaaa aggtagccgc ctcatgcaca tgttacgtcg ctggttaggc gatacagatt
     8461 ttgccgcagg actaaaaatt tattttgaaa aacatcaata tcaaaatact attggtcgtg
     8521 acctttggaa tgcactttct caaacctcag gaaaagatgt tgcagctttc atggattctt
     8581 ggttagagca accaggatac cctgtgatgg cagccaaaat agaagaagat gagctcattt
     8641 taactcaaaa acaattcttt attggtgaac acgaagataa gtctcgcttg tggcaaattc
     8701 ctcttaatag taattgggaa ggaatcccag agatattaac cgaggaaacg gtagtaatcc
     8761 ccaattttag tcagttagct gaaaagaaca aagaaaatgg tgcactgcgt ttcaatactg
     8821 aaaatactgc acattacatt actaactatc aaggtcaact cttagagcat atcatttctg
     8881 atcttcctct tatggataac atttctaaac tacaaattgt acaagaacgt catttacttg
     8941 ctgaaagtgg tatgatttca tatagtagtt taataccttt agtctctctt ttgagccaag
     9001 agacatcata tcttgttaac tctgctatta agagtgttat tgatggttta tccttattcg
     9061 tccaggaaga tagccaagat gaattcgact ttaaagaatt tgttaataaa ttatcagcat
     9121 ttaattttaa tagacttggc tttgaaaaaa gagaaggaga aggagatgat agcgaaatgg
     9181 tccgtcatct atccctaagc cttgctctct actcagataa tgaacatgct attgaagaag
     9241 ctcaccatat ttttaaagcc cacgaaaata atatcgctgc tataccagct gcaattcgcc
     9301 tgttagttct taccaatgaa atgaagcatt tcgaatccaa agaacttagc cacttgttat
     9361 tagagactta tagtactact accgatggga atttcaaacg acaactagcg agtgctttat
     9421 ctcatactac agatagcaag acactcaaga aattattgtc agattggaaa aataaagata
     9481 ttgtgaaacc acaagactta gctatgagtt ggtatgctac tttccttaaa aattctttta
     9541 cacaagaaag tgtctgggag tgggcccaag aaaactggga atggatcaaa gcaactctag
     9601 gtggtgatat gagttttgat aaatttgtga tttatccctc aagtagtttc aaaacagaag
     9661 aacgccttga acaatataaa aactttttcg agcctcaatt aagcgatatg gcgattagcc
     9721 gtaatatttc aatgggaatt aaagaaattt ctgctcgtgt ccttttaatc acgaagcaaa
     9781 aagaagaagt tatcaatact atcaaaaaat atatctaata aactcaaata gagattgatg
     9841 gattattcaa tctctatttc tttgtctgtg tcatgaaatt agttgacata aaacatactt
     9901 tatttaggat ttattaagca aagtttggta ttctaatatt aatagttcag ataaaatatg
     9961 tacatcgaaa ggatatacta tgataaaaat tttattaatt gaagatgact tgagtctttc
    10021 caattccgtt tttgatttcc ttgatgactt tgccgatgtc atgcaaattt ttgatggaga
    10081 agagggtctt tacgaagccg aaagtggagt ctatgatctg attcttctag atctcatgtt
    10141 acctgaaaaa aacggcttcc aagtgttgaa agagctacgt gaaaaaggta ttacaacccc
    10201 tgtattaata atgaccgcta aagaaagcat tgatgataag ggacaaggtt tcgacttagg
    10261 tgctgatgat tatctaacaa aaccttttta cctagaagaa ctaaaaatgc gaattcaggc
    10321 tcttttaaag cgttctggaa aatttaatga taacagccta atttatggtg atattcgcgt
    10381 agacatgtct actaactcaa cctttgttaa tcaaacagaa gttgagctct taggtaaaga
    10441 gtttgatctt ttagtctatt tccttcaaaa tcaaaatgtt atcttaccga aatctcaaat
    10501 ctttgatcgt atttggggat ttgatagtga tacaacgatt tcagttgtag aagtctatgt
    10561 ctctaaggtt cggaaaaaat taaaaggaac acttttttca gaaaaccttc aaactttgcg
    10621 tagtgtagga tatattttaa agcatgtcga aaccgattaa gataagtaaa ttcaaaaaaa
    10681 atgtctcaga tagtcatttt atccattttt tcacagtttt tagtggtatt tttctggtta
    10741 tgactgttat catcctccaa gtcatgcgct atggtgttta ttcttctgtt gatagtagtt
    10801 taaagtatat cagtacccat cctaaaaatt acatcaatat ggttatgtca cgtactgctg
    10861 cctacctaga taactccaat atcgcaagtg tcaagttaaa acccggcggt cagacagtag
    10921 ctaatactga tattattctt tttacatcag aagaagaagt cattaacta
//
LOCUS       DATROD010000057        10851 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.057, whole genome shotgun sequence.
ACCESSION   DATROD010000057 DATROD010000000
VERSION     DATROD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10851
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.057"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            319..606
                     /locus_tag="VBC74_001324"
     CDS             319..606
                     /locus_tag="VBC74_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEO6942270.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILSDNGATVVESKDWE
                     KRRLAYEIQDFTEGLYHIVNVEAEDAVALNEFDRLSKINGDILRHMIVKVD"
     gene            618..1109
                     /locus_tag="VBC74_001325"
     CDS             618..1109
                     /locus_tag="VBC74_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEO6942271.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNQAVATFSLAVNRNFKNQSGER
                     EADFINCVIWRQQAENLANWAKKGALVGITGRIQTRNYENQQGQRVYVTEVVAESFQL
                     LESRATREGGSPNSYNNGGYNNAPSNNSYSASSQQTPNFSRDESPFGNSNPMDISDDD
                     LPF"
     gene            1154..1393
                     /locus_tag="VBC74_001326"
     CDS             1154..1393
                     /locus_tag="VBC74_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEO6942272.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            1563..2507
                     /locus_tag="VBC74_001327"
     CDS             1563..2507
                     /locus_tag="VBC74_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEO6942273.1"
                     /translation="MKQMFLSTAIEFKEIETFEPGAWINLVNPSQEESEQVADQFGID
                     IDDLRAPLDVEETSRISVEDDYTLVIVDVPTYEERNNKSYYMTIPMGIIVTDNAVITT
                     CLEHLTLFDHFYRRRVKNFYTFMKTRFVFQLLYRNAELYLQALRTIDRQSDKIEAQLE
                     SATRNEQLIDMMELEKSIVYLKASLKFNERIVKKLTSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMANIYENVLNAMTETTASIIGNNQNTIMKTLALVTMTLDIPTVIFSAYGMNF
                     KNNWMPLNGLAHGFIYVVLLAFLMSSFVVFYFIRKKWF"
     gene            2532..3203
                     /locus_tag="VBC74_001328"
     CDS             2532..3203
                     /locus_tag="VBC74_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HEO6942274.1"
                     /translation="MTLQDLTKKNQEFVHIATNQLLADGKSDAEIKAILEEHLPEIID
                     NQKKGITARSLLGAPTTWAASFTERPEDKARVSVQKNTNPWLMWLDTSLLFLGLVTAL
                     NGLMLLFGQSNVNTGLISILTLGFGGGAAMYVTYYYIYRHMGKPKSERPGWLKSFAVL
                     ALVMLVWFALFAVVPLLPATINPKLPEVVLFIIALASFGLRFYLQRKYNIQSSMAPVA
                     PQQRR"
     gene            3335..6163
                     /gene="uvrA"
                     /locus_tag="VBC74_001329"
     CDS             3335..6163
                     /gene="uvrA"
                     /locus_tag="VBC74_001329"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEO6942275.1"
                     /translation="MQDKLMIRGARAHNLKNISVDIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSVEQIVDKVLALPERTKMQILAPIIRRKK
                     GQHKSTFEKIQKDGYVRVRIDGDIHDVTEVPELSKSKMHNIDIVVDRLINKEGIRSRL
                     FDSVEAALRLSDGYVVIDTMDGNELLFSEHYSCPECGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDIDLVIPDRSKTLREGALVPWNPISSNYYPTMLEQAMTQFGVDVDTP
                     FEKLSKAEQDLALYGSGEREFHFHYINDFGGERNIDLPFEGVVNNINRRYHETNSDYT
                     RNVMREYMNELKCNTCHGYRLNDQALCVRVGGEEGLNIGQVSDLSIADHLELLETLRL
                     SSNEQLIARPIIKEIHDRLSFLNNVGLNYLNLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYVLDEPSIGLHQRDNDRLIDSLKKMRDLGNTLIVVEHDEDTMMAADWLIDVGPGA
                     GAFGGEIVASGTPKQVAKNTKSITGQYLSGKKVIPVPSERRVGNGRFLEIKGAAENNL
                     QNLDVKFPLGKFIAVTGVSGSGKSTLINSILKKAVAQKLNRNSDKPGKYVSLEGIEYV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     ESCSGDGIIKIEMHFLPDVYVPCEVCHGTRYNSETLEVHYKEKNIAQILDMTVNDAVT
                     FFAAIPKIARKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHADDIARLLKVLDRFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGIGGGQ
                     IVAIGTPEEVAENPKSYTGYYLKEKLAR"
     gene            6499..7998
                     /locus_tag="VBC74_001330"
     CDS             6499..7998
                     /locus_tag="VBC74_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO6942276.1"
                     /translation="MTTKAKHDIHGKPYNRTAMITLLLIATFAGVLNQTSLGTAIPTL
                     MNSFNISLSTAQQATTWFLLANGIMIPVSAYLATRFSTKWLYVTSYVVLLIGLLMTTL
                     APTSNWNLFLVGRIIQAISVGISMPLMQVVMVNVFPPEQRGAAMGLNGLVVGLAPAIG
                     PTLAGWILKQEFHFAGHDLTWRAIFLLPLLILTVTTILSPFVLKDVVDNKSVKLEVPS
                     LILSIIGFGSFLWGFTNVATYGWGDIGYVISPIMVGIIFIALFIHRQLKLETPFLDIR
                     VFKNKQFSVTTAAIALSMMAMMGVEMMLPLYLQNVHGLSALDSGLALLPGALMMGIVS
                     PISGAVYDKVGARRMAMIGFTILGVATLPFVFLTTTTPDHFITLLYAVRMFGIAMVMM
                     PLTASAMSALPPHEAAHGTAANNTARQIASAVVVALLSSVAQNIITNNKPSKDLLTMN
                     PLKYANQMLNASLDGFHVSFAIGFVFAVLGLLVSLFLRKGKIIETEKEV"
     gene            8000..8716
                     /locus_tag="VBC74_001331"
     CDS             8000..8716
                     /locus_tag="VBC74_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEO6942277.1"
                     /translation="MIILIIALATILTFVTWMLIRPKALRYILGLLSLALLFASVWML
                     TDHFINHTGMTIETKTKTREIYSAGDSKAPFGLMIYKEIGTHSSNNVLVYRTDLNQKE
                     AKAHFVPNTKEVTSAVKKSASYQLADVKKATVTTKTKRYVWKDSLSKLLYGFAGENGE
                     LISQKSVAKIPRKTWLVLTKEQSEHLESAAAKLKEQMTSQAKVNPVKAMEIKTLAKTN
                     PEAYAEMQVKALKEALNIKE"
     gene            8816..9883
                     /locus_tag="VBC74_001332"
     CDS             8816..9883
                     /locus_tag="VBC74_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEO6942278.1"
                     /translation="MGFEQRLTRCQTAISQLSCDALLITNLTNIFYLTGFSGTNATVL
                     ISPKHRIFVTDSRYALIAKNTVREFDIIISREPLAAILKIIRDDALIAIGFETDISYH
                     MYKHMVEVFEDYRLIEAPSVVEKLRMIKDVTELAIIRQACRISDQAFLDVLDFIKPDR
                     TTELQVANFLDFRMRELGATGPSFDFIVASGYRSAMPHGVASQKTIQSGETLTLDFGC
                     YYQHYVSDMTRTIHIGHVTDQEREIYDIVLKSNQAIIDNVKSGMKRCDYDYLARQVIE
                     NSGYGNHFTHGIGHGMGLDVHEIPYFGKSEGVIASGMVVTDEPGIYLDNKYGVRIEDD
                     LLITETGCEVLTSAPKELIVL"
     gene            9895..10347
                     /locus_tag="VBC74_001333"
     CDS             9895..10347
                     /locus_tag="VBC74_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEO6942279.1"
                     /translation="MNRLSWEDYFMANAELISKRSTCDRAFVGAVLVKNNRIIATGYN
                     GGVSETDNCNEVGHYMEDGHCIRTVHAEMNALIQCAKEGISTNNTEIYVTHFPCINCT
                     KALLQAGVKKITYKANYRPHPFAIELMEAKGVAYVQHDVPEVTLGMEE"
BASE COUNT         3490 a         1736 c         2159 g         3466 t
ORIGIN      
        1 aacgaaaact taaaccaata aagaattctt taaataatat gttgaaggtg aggtgtaaac
       61 ctcgcctttt tattttttaa ttgaattata gaatggggat tgaacataag tcagtaaaag
      121 aaaatggcta cataggtttg atataaggca tatttcaata aagaaaaata aaaaatgtct
      181 ttaaatgata tcagggaaat aaaaacataa aaaccttgca ttattaactt atttctacta
      241 taataatatc ttgtgagcat aacctcactt actcatgaaa aattcatgag tcattagacc
      301 aaaaggagga acatatcaat ggctaaatac gaaattcttt atattattcg tccaaacatt
      361 gaagaagaag ctaaaaacgc tttggtagca cgttttgatt ctatcttatc tgacaacggt
      421 gcaactgttg ttgaatcaaa agattgggaa aaacgtcgtc ttgcatacga aatccaagat
      481 ttcactgaag gactttacca tatcgttaac gttgaagctg aagatgcagt agctcttaat
      541 gagtttgatc gtctttcaaa aatcaacggt gacattcttc gtcacatgat cgttaaagta
      601 gactaagaag gtagtttatg attaataatg tagtacttgt aggtcgcatg acccgtgatg
      661 cagaacttcg ttatacacca agtaatcaag cggtagccac tttttcactt gcagttaatc
      721 gtaattttaa aaatcaatct ggcgaacgtg aggctgattt tattaactgt gttatttggc
      781 gccaacaagc tgaaaacttg gctaactggg caaaaaaagg tgctttggtt ggaattacag
      841 gtcgtatcca aacgcgtaat tatgaaaacc aacaaggtca acgtgtctat gtaacagaag
      901 ttgttgcgga aagtttccaa ttattggaaa gtcgtgctac acgagaaggt ggttcaccta
      961 actcttataa taacggtggc tataacaatg ctccgtcaaa taatagttat tcagcttctt
     1021 ctcaacaaac acctaatttt agtcgtgatg agagtccgtt tggtaactca aatccaatgg
     1081 atatttcaga tgatgatcta ccattctaat ataatagatg gacagaattt aaaactataa
     1141 aggagaaata aacatggctc aacaacgtcg tggcggattc aaacgccgta aaaaagttga
     1201 ttatatcgca gctaacaaga ttgaatatgt tgattacaaa gatacagagc ttcttagccg
     1261 tttcgtttca gaacgtggaa aaattttacc acgtcgtgta acaggaactt cagctaaaaa
     1321 ccaacgtaaa gtaacaacag caatcaaacg tgctcgtgtt atggctctta tgccttacgt
     1381 aaatgaagat taatataaaa tagccctagc ggctattttt ttattctttt taccattatt
     1441 attggtattt acgagtcaaa taatagaaaa atttaggata ccaccgtatt aaccgtgcaa
     1501 aacaaatggt tttcctgtat aatactaagg tatggatttt ttataacaaa ggaggaatcg
     1561 ccatgaagca aatgtttctt tcaacggcaa ttgaatttaa agaaattgaa acattcgagc
     1621 caggtgcttg gataaatctg gttaatccct cccaggaaga atccgaacaa gtagctgatc
     1681 aatttggtat tgatattgat gatttacgtg ctcccctcga tgtggaagaa acttcccgta
     1741 tctcggttga agatgattat actttagtta ttgttgacgt tcctacctat gaagaacgca
     1801 ataacaaaag ttattatatg actattccta tgggcattat cgttactgac aacgctgtta
     1861 ttactacttg cctagaacat ttgacgctgt ttgatcactt ttatagacga cgtgtcaaaa
     1921 atttttatac ctttatgaag acacgttttg ttttccaatt gctttatcgc aatgccgaat
     1981 tatacttaca ggctttgcgt actatcgata ggcagagcga taaaattgaa gcacaactag
     2041 agagtgcaac tcgaaatgag caattgatcg atatgatgga attggaaaaa tctattgtct
     2101 atctaaaggc ctctttaaaa tttaatgagc gaatcgtcaa aaaattgacc agtagtacca
     2161 gtagtttaaa aaaatacatt gaagatgaag atctgcttga agatacattg atcgaaactc
     2221 agcaggctat tgaaatggca aatatctatg aaaatgtctt aaatgccatg acagaaacga
     2281 ctgcttcaat catcggtaat aatcagaata ccattatgaa aaccttagcc ctagtaacta
     2341 tgacattgga tattccaacg gttatttttt cggcgtatgg gatgaacttt aaaaataatt
     2401 ggatgccttt gaatggtctc gcacatggct ttatctatgt tgttcttttg gcctttttga
     2461 tgagttcatt tgttgtattt tattttattc gtaaaaaatg gttttaatag tatattaatc
     2521 aaggagttca catgacttta caagacttaa ctaaaaagaa tcaagagttt gttcatattg
     2581 caactaatca acttttagct gatggaaaat cagatgcaga aatcaaagct attttagaag
     2641 aacatctccc agagattata gataatcaga aaaaaggaat taccgcacgc tcactcttag
     2701 gagcaccaac tacttgggca gcctcattca ctgagcgacc tgaggataaa gctagagtat
     2761 ctgtacaaaa aaataccaat ccttggttga tgtggttaga tacttcctta ctatttcttg
     2821 gcttagtaac tgctttgaat ggtttaatgt tgctatttgg tcaatctaat gtcaatacag
     2881 ggcttatttc aattttaact cttggttttg gtggtggtgc ggccatgtac gttacttatt
     2941 actatattta tcgccatatg ggtaaaccaa aatctgaaag accaggctgg cttaaatctt
     3001 tcgcagtact ggcactagtt atgttagttt ggtttgcctt atttgcagta gtacctcttc
     3061 tacctgcaac tattaatcct aaacttcctg aggttgtttt gtttattatt gctttagcat
     3121 cgtttggttt gcgcttttat ttacaacgta aatataatat tcaaagctca atggcaccag
     3181 tggcaccaca acaaagaaga taataacaaa gagtatgaac gtattagttc atctcttttt
     3241 tctatgaaat ttaaaactaa aaaaatactg actttattaa aatttgatat aataagtcaa
     3301 cactattcaa aaaggagtgc tgaggcacaa cattatgcaa gataaattaa tgataagagg
     3361 agcaagagct cacaatttaa aaaatataag tgtcgacatt ccacgagaca agttagttgt
     3421 cgttacggga ctatcaggat caggtaagtc tagtttggcc tttgacacta tctatgcaga
     3481 aggacaacgt cgttacgttg aaagtctttc tgcttacgcg cgccaatttt tgggaaatat
     3541 ggaaaagcct gatgtagatt ctattgatgg ccttagtcca gctatttcta ttgatcagaa
     3601 aacaacaagt aagaatcctc gctctactgt tgggactgta acagaaatta acgattattt
     3661 acgtttgctt tatgctcgtg ttggaacccc atattgtatc aatggtcacg gagccatcac
     3721 agcatcttct gtagagcaaa ttgtggataa ggtactagcg cttccagagc gaacaaagat
     3781 gcaaatttta gccccaatta tacgtcgaaa aaaaggacaa cacaaatcaa catttgagaa
     3841 aatacaaaaa gatggttacg ttcgtgttcg tattgatggt gatattcacg atgttactga
     3901 ggtgcctgaa ttatcaaaaa gtaagatgca caatattgat attgtagtag atcgattaat
     3961 taataaagag ggcattcgtt cccgcttatt tgattccgtt gaagcggctc tacgtttgtc
     4021 ggatggttat gtcgttattg atacaatgga tggcaatgag ttattgtttt cagagcatta
     4081 ttcttgtcct gaatgtggtt ttactgttcc tgaattagag ccacgccttt tttcttttaa
     4141 tgctcccttt ggttcttgtc cgacctgtga tggcttagga attaagttag aagtagatat
     4201 agatttggtt attccagata gaagtaagac ccttagagaa ggggccttag ttccttggaa
     4261 tccaatttca tcaaattatt atccaacgat gctagagcaa gctatgactc aatttggtgt
     4321 tgatgtggat acgccatttg aaaaattaag caaggcggag caggacctag ccctatatgg
     4381 ttcaggcgaa cgagagttcc atttccatta tattaacgat tttggaggcg agagaaatat
     4441 tgatttgcct tttgaagggg ttgtcaataa tattaatcgt cgttaccatg aaactaatag
     4501 cgattacaca cgcaatgtta tgcgagaata tatgaacgag ctaaaatgta acacttgtca
     4561 tggctatcgt ctcaatgatc aagcactttg cgtaagagtt gggggagaag aaggtcttaa
     4621 tattgggcaa gtctcagatt tatcgatagc agaccattta gaacttttag aaacattgcg
     4681 tttatcttca aatgaacaat taattgctcg accaattatc aaagaaatcc atgaccgtct
     4741 aagtttctta aataatgttg gacttaatta tttaaatctg tcacgttctg caggaacact
     4801 ttccgggggc gagagtcaac gtattcgttt ggctactcaa attgggtcta atttatcagg
     4861 tgtcctttat gtacttgatg agccgtccat tggacttcat cagcgagaca atgaccgttt
     4921 gattgatagt cttaagaaaa tgcgtgattt gggtaatacg cttattgttg ttgaacatga
     4981 tgaagataca atgatggcag ctgattggtt aatcgatgtg ggacctggcg ctggtgcgtt
     5041 tggtggtgag attgtggcct ctggtacacc gaagcaagta gctaaaaata ctaaatctat
     5101 tacaggacaa tatttgtcag gtaagaaagt gattcctgtg ccatctgaac gaagagtagg
     5161 taatggtcgc tttttagaaa tcaaaggtgc ggcagaaaac aacctacaaa atttagatgt
     5221 caagttccct ctcggtaaat ttattgccgt aactggtgtc tcaggttctg gaaaatcaac
     5281 cttaattaat agtattttga aaaaagctgt tgcgcagaag cttaaccgta actcagataa
     5341 accaggtaaa tatgtttctt tagaaggcat tgaatatgtt gatcgattga ttgatattga
     5401 tcaaagtcca attggtcgga ctccacgttc taatcccgca acctatacag gtgtttttga
     5461 tgatattcgt gatctttttg ctcaaactaa tgaggcaaag attcgaggtt ataaaaaagg
     5521 acgcttctca tttaacgtca aaggtggacg ttgtgagtct tgttctggcg atggtatcat
     5581 caaaattgag atgcattttt taccagatgt ctacgttcct tgtgaagtat gccacggcac
     5641 gcgctataat agtgagactc tggaagtaca ttacaaagaa aaaaatattg cacaaattct
     5701 tgatatgaca gtgaatgatg cagttacatt ttttgcagct attcctaaaa ttgctcgtaa
     5761 gttacagact attaaggatg ttggtttggg atatgtcact ttggggcaac cagctacaac
     5821 cttatcagga ggagaagctc agcgaatgaa gctggcaagt gaattacata agcgttcgac
     5881 aggtaagagt ctttacatat tagacgaacc aacaacgggg cttcatgcag atgatattgc
     5941 tcgtttactt aaggttttgg atcgttttgt agatgatggt aatacagtgc ttgttattga
     6001 gcataacctt gatgttatta aaactgctga ccacattatt gatcttggcc ctgaaggagg
     6061 tattggtggc ggacaaattg tagctatcgg aacaccagaa gaagttgctg aaaatcctaa
     6121 atcctataca ggatattatt taaaagagaa gttagcaaga tagtttattg ggagctgagt
     6181 aatattactc agttcctttt tgtaagctaa cattgcgtag aagtcattaa atggagaacg
     6241 ctttattagg aaaaatagtg gggagatatt ttaaaaaaag gaaataatga tgttttgaaa
     6301 aaaatagttt ctatgagaat ctttttcttg acaataaaat ttagctatta taaaatagtt
     6361 ctcacgataa ctaaaataat gattttttca gaaaataata ttatttcttg attaaaaaat
     6421 caaaaaataa ccttataaga cctatttgtt attcttaaaa gatattagtt aaaatcagaa
     6481 atagagattg gagataaaat gactacaaaa gcaaaacatg atattcatgg caaaccatat
     6541 aatcgtacag ccatgatcac cttattgttg attgctacat ttgcaggggt attaaatcag
     6601 acaagtttgg gaacggctat cccaactttg atgaatagct ttaacatttc gttgtcaacg
     6661 gctcaacaag caacaacatg gtttttatta gcgaatggga ttatgattcc tgtttcagct
     6721 tatttagcga cacgtttttc aacaaaatgg ttgtatgtga catcctacgt tgttttactt
     6781 attggactgt taatgacgac gttagcacct acatctaact ggaacctttt tttagtagga
     6841 cgtattattc aagcgatttc tgtagggatt tctatgcctt tgatgcaggt tgttatggtg
     6901 aatgtttttc ctccagagca gcgtggtgcc gctatgggac ttaatggttt ggtggttggt
     6961 cttgccccag ctattgggcc tacattagca gggtggattt taaaacaaga atttcatttt
     7021 gcagggcatg atttaacatg gcgtgcaatt ttccttcttc ctttactaat tttaacggtc
     7081 actacaattt tatccccctt tgttctaaaa gatgtggttg ataataagtc agttaaattg
     7141 gaagtgcctt cccttatcct ttcaataatt ggctttggta gctttttgtg gggcttcaca
     7201 aatgtggcaa cttatggatg gggagatatt ggatatgtta tttctcctat tatggttggt
     7261 attattttta tcgccttatt cattcatcgt caattaaaac tagaaacacc gttcttagat
     7321 atccgtgttt tcaaaaataa acaattctca gtaacaacag cagctattgc actttcaatg
     7381 atggcgatga tgggtgttga aatgatgcta ccgctttact tgcaaaatgt tcatggtctc
     7441 tctgcacttg attctggttt agctttatta ccaggtgcct tgatgatggg gatagttagt
     7501 ccaatttctg gagctgtata tgataaagtt ggtgcaagac gtatggctat gattggtttt
     7561 accatactag gtgtagcgac gttacctttt gttttcttaa cgactacaac accagatcat
     7621 tttattaccc tcttatatgc agtacgtatg tttggtattg ctatggttat gatgccattg
     7681 acagcaagtg cgatgagcgc acttccacca catgaggccg cacatggaac agcagctaat
     7741 aatactgctc gtcaaattgc ttcagcagtt gtagttgcat tgctttcaag tgttgctcaa
     7801 aatattatca ccaacaataa accatcaaaa gatttgctaa caatgaatcc tttaaaatat
     7861 gcaaatcaga tgttgaatgc tagtcttgat ggcttccatg tttcttttgc cattggtttt
     7921 gtatttgcgg ttcttggttt actggtatct cttttcctga gaaaagggaa aattattgag
     7981 acggaaaagg aggtatagca tgattatttt gattattgca ctggcaacta ttttgacatt
     8041 tgtcacatgg atgttgattc gtccaaaagc tctacggtat attctaggtc ttctctcatt
     8101 agctttgttg tttgcctcag tttggatgtt aacggatcac tttattaatc atacaggtat
     8161 gactattgaa actaagacaa agacacgtga gatttatagt gcgggtgata gtaaggctcc
     8221 atttggtctc atgatttata aagagattgg tactcattcg agtaataatg ttttagttta
     8281 tcgaacagat ttaaatcaaa aagaagcaaa agctcacttt gttccaaata caaaagaagt
     8341 gacaagtgct gtgaaaaaat ctgctagcta ccaattagct gatgtgaaaa aagccacagt
     8401 aacaacaaag actaaacgtt atgtgtggaa agatagtctt tcgaaactac tttatggctt
     8461 tgcaggtgaa aatggagaat tgatttctca gaaatcagtt gctaaaatac cacgaaaaac
     8521 ttggctagtt ttgactaaag agcagtccga acacttagaa tcggctgcgg ctaaactaaa
     8581 agaacaaatg acttcgcaag caaaagtaaa cccagtgaaa gctatggaga taaagacatt
     8641 agctaagaca aatccagaag cttatgctga gatgcaagtt aaagcattaa aagaagctct
     8701 taatataaaa gaatagtaaa gtaaaagcaa ctgaaatgag ttgcttttct tatagctaaa
     8761 tgttttaaaa gatgataaaa ttaacctaat taaggttaga atagcgaggt ttcttatggg
     8821 ttttgaacaa cgtctaacta gatgtcaaac agcaatttca caattatcat gtgacgcact
     8881 cttaataaca aatttaacaa atatttttta tttgactggt ttttcaggaa ctaatgcaac
     8941 ggtactcatt agtccaaaac accggatttt tgttacagat tcacgatatg ccttgatagc
     9001 aaagaatact gtaagagagt ttgatattat cattagcaga gaaccacttg cggctatttt
     9061 gaaaattatt cgcgatgacg cattgatagc gattggtttt gaaactgata tttcttatca
     9121 tatgtacaaa catatggtgg aagtttttga agattatcgc ttgatagaag ctccatctgt
     9181 tgttgaaaag ttacgtatga ttaaagatgt aacagaactt gcgatcatcc gtcaagcctg
     9241 tcgtatttcg gaccaagctt ttttagatgt gttggatttt ataaagcctg atcgaacaac
     9301 tgaattgcaa gttgctaatt ttttagactt tcgcatgaga gaattaggag caactggtcc
     9361 ttcatttgat tttattgtag catctggata tcgatcagcg atgccacatg gggttgcgag
     9421 tcagaagact attcaatcgg gagaaactct gactttagat tttggttgtt actatcagca
     9481 ctatgttagt gatatgacac gtacgattca tatcggtcat gtcactgatc aggaacgcga
     9541 gatttacgat atcgttttga aaagtaatca agctattatt gataatgtaa aatcaggaat
     9601 gaagcgttgt gattatgatt acttggcgcg acaagtcatt gaaaattctg gatacggtaa
     9661 tcattttacc cacggtatag ggcatggtat ggggcttgat gttcatgaaa ttccttattt
     9721 tggtaagtca gagggagtga ttgcatctgg gatggtagtg actgacgagc caggaatata
     9781 tctcgataat aaatacggtg ttcgtattga agatgattta ttaataacgg aaacaggttg
     9841 cgaagtattg acatctgcac cgaaagaatt aattgtactt taaaggaaaa tagaatgaat
     9901 agactttctt gggaagatta ttttatggca aatgcagagt tgatttctaa acgttctacc
     9961 tgtgaccgtg cttttgttgg agctgtttta gtcaaaaata atcgcatcat tgctacaggt
    10021 tataacggag gagtctctga gacagataac tgtaacgaag tgggacatta tatggaagat
    10081 ggtcactgta ttaggactgt tcatgctgag atgaatgccc ttattcagtg tgctaaagaa
    10141 ggtatttcta ctaataatac agaaatttat gtgacgcatt ttccttgtat caattgcacc
    10201 aaggcgctct tgcaagccgg ggttaagaaa attacttata aagctaatta tcgcccacac
    10261 ccatttgcta ttgaacttat ggaagcaaaa ggagttgctt atgttcaaca tgatgttcca
    10321 gaggtaacac tagggatgga agagtaaaga ggaagataga gtatatgcct atcttccttg
    10381 aaggttttat cgtttaagct aagggccagt tatgttaaat atattgaacg agataattct
    10441 caaaattggt caattgttag tcatgggatt actgaaaatg gcctaaaaaa ggtgatcacc
    10501 agttaatagt cattattcct aatgattttt cacagaaagt tttagatgtt gataatagaa
    10561 tagctgatcc atctattatc aacatctaaa attagtactg ctgggaatct cgaattgcaa
    10621 aaataattta caaaaaagag aaaagatatt gttgctgacc tcaatagatg taaatccagt
    10681 ttatgctaat atggcatttg attgggtgta tgataactat aaagatgata tcatcaatta
    10741 tggacaagaa actatcaaaa atattggtaa atccatttca gctaataata gtttaggtac
    10801 tttatataat taaggagcaa gatttgaaaa cggaaataga aacaaataat c
//
LOCUS       DATROD010000058        10844 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.058, whole genome shotgun sequence.
ACCESSION   DATROD010000058 DATROD010000000
VERSION     DATROD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10844
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.058"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            148..915
                     /locus_tag="VBC74_001334"
     CDS             148..915
                     /locus_tag="VBC74_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEO6942280.1"
                     /translation="MIDVENILEKMKPNQKINYDWVMQQMVKQWQASDIRPKILMHVC
                     CAPCSTYTLEYLSQWADVTIYFANSNIHPKDEYYRREYVTQKFVHDFNKNTGYSVQFL
                     SAPYEPNEFFKIVHGLEEEPEGGDRCKVCYDFRLDKTAEKAVELGFDYFGSALTISPH
                     KNSQTINTIGIDVQKIYDTQYLPSDLKKNKGYQRSVEMCKDYDIYRQCYCGCIFGAKD
                     QGINLLQIKKDAKAFVSDKDGKEEFPNIRFTFNGKSM"
     gene            complement(974..2314)
                     /locus_tag="VBC74_001335"
     CDS             complement(974..2314)
                     /locus_tag="VBC74_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEO6942281.1"
                     /translation="MYQTQTNKEKFVLFLKLFIPVLIYQFANFSATFIDSVMTGQYSQ
                     LHLAGVSTASNLWTPFFALLVGMISALVPVVGQHLGRGNKEQIRTEFHQFLYLGLILS
                     LILFLIMQFIAQPVLGSLGLEDEVLAVGRGYLNYMLIGIMPLVLFSICRSFFDALGLT
                     RLSMYLMLLILPFNSFFNYMLIYGKFGMPRLGGAGAGLGTSLTYWAIFIVIIIVMSLH
                     PQIKTYHIWTLERIKAPLIIEDIRLGLPIGLQIFAEVAIFAVVGLFMAKFSSIIIAAH
                     QAAMNFSSLMYAFPLSISTALAITISFEVGAERFQDANTYSRIGRLTAVGITSGTLLF
                     LFLFRENVAAMYNSDPHFVAITAQFLTYSLFFQFADAYAAPVQGILRGYKDTTKPFMI
                     GAGSYWLCALPLAVILEKNSQLGPFAYWIGLITGIFVCGLFLNQRLQKIKKLYY"
     gene            complement(2414..2830)
                     /locus_tag="VBC74_001336"
     CDS             complement(2414..2830)
                     /locus_tag="VBC74_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6942282.1"
                     /translation="MVMKAIEIYMNINGRGAEAITFYEEAFNTKAEHVTTFAQGWPDC
                     PPELEHLIMNAQFNIDGIRMQLSDNNPIFDFQIGQNMTAALIVESVEEAQELFNKLSR
                     DAMNIKMPLQAVPWSPAYGNVTDKYGMTWQINTELS"
     gene            complement(2834..3331)
                     /locus_tag="VBC74_001337"
     CDS             complement(2834..3331)
                     /locus_tag="VBC74_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEO6942283.1"
                     /translation="MIIDFKTVSTKGLETSPVAEALAGLRANEARYFWNKFKVAYETF
                     PVSDYPELMADINHLLKNERNLVFAAKPLEISQLFLENRILWTHVFYEDGLAINILYE
                     LDNPKKRAVGLKLSDGMDIPIELADKFKFARQKSKLAGIIRGSYFTLKGENETLMQRW
                     LDNMK"
     gene            3553..4149
                     /locus_tag="VBC74_001338"
     CDS             3553..4149
                     /locus_tag="VBC74_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HEO6942284.1"
                     /translation="MTKKIFLFICATLLVGIALYTPTTSIFNHNNVYAATKKAKIKFN
                     KTQKKIVKKAREYAKSGHMSKDSIIEKLKKDSKKYRQEDINFVINNLKVDYKKNALIS
                     AKIYSKTMNLSKQSIFEQLYSESPDKATHSDKFTKEESQYAIDHLKVDFKENALETAK
                     SYQSSSSLSKEEIYKQLTSTLGDKFTNDEAQYAVDHLK"
     gene            4338..5443
                     /locus_tag="VBC74_001339"
     CDS             join(4338..4614,4614..5443)
                     /locus_tag="VBC74_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612000.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSag2 family transposase"
                     /protein_id="HEO6942285.1"
                     /translation="MSRKVRRHFTDDFKQQIVDLYNVGRKRSSLIKVYELTPSTFDKW
                     VRQAKTTGSFKSVDNLTDEQRELIELRKHNKELEMQLDILKQAAVIMAPKREIITANK
                     DKYSISKMCRWLNMPHSSYYYQAVESVSETEFEETIKRIFLDSESRYGSRKIKICLNN
                     EGITLSRRRIRRIMKRLNLVSVYQKATFKPHSRGKNEAPIPNHLDRQFKQERPLQALV
                     TDLTYVRVGNRWAYVCLIIDLYNREIIGLSLGWHKTAELVKQAIQSIPYALTKVKMFH
                     SDRGKEFDNQLIDEILEAFGITRSLSQAGCPYDNAVAESTYRAFKIEFVYQETFQLLE
                     ELALKTKDYVHWWNYHRIHGSLNYQTPMTKRLIA"
     gene            5667..6799
                     /locus_tag="VBC74_001340"
                     /pseudo
     CDS             5667..6799
                     /locus_tag="VBC74_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558285.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6839..7078
                     /locus_tag="VBC74_001341"
                     /pseudo
     CDS             6839..7078
                     /locus_tag="VBC74_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7277..9745)
                     /locus_tag="VBC74_001342"
     CDS             complement(7277..9745)
                     /locus_tag="VBC74_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEO6942286.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGLATKDNQIAYIDD
                     SKGKVKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYHFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPGRRKAPIPDVTPNP
                     GQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEEDGL
                     IFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTDDNDSGSDHSK
                     PSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHFHY
                     IGFGELEQYELDEVANWVKAKGQADELVAALDQEQGKEKPLFDTKKVSRKVTKDGKVG
                     YIMPKDGKDYFYARYQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSSLP
                     MHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRNQIATIKYVMQHPEVRPDVWSKPGHE
                     ESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFAAPDGYIFDPRDVLAKETF
                     VWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPEEKQQAD
                     KSNENQQPSEASKEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIFQPE
                     GVQFYNKNGELVTYDIKTLQQINP"
     gene            complement(9758..10678)
                     /gene="lmb"
                     /locus_tag="VBC74_001343"
     CDS             complement(9758..10678)
                     /gene="lmb"
                     /locus_tag="VBC74_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEO6942287.1"
                     /translation="MKKVFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVNVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGHLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
BASE COUNT         3519 a         2104 c         1851 g         3370 t
ORIGIN      
        1 aacaaaatgt actgtaatct ttacgaaata aatactattc cccaacatct attgtatctt
       61 ccaatacttg aataaaaccc caatactcct agtctaagca attattttat gttacacttt
      121 aaacaagtga attattgagg agtttttatg atagacgttg agaatattct tgaaaaaatg
      181 aaacctaacc aaaaaatcaa ttacgattgg gtcatgcaac aaatggttaa gcagtggcaa
      241 gctagtgata tccgtccgaa aattctgatg catgtttgct gtgctccttg tagtacatat
      301 acccttgaat acttatcaca atgggcagat gttaccatct attttgctaa ttcaaatatc
      361 catcctaaag atgaatatta tcgccgcgaa tatgttactc aaaaatttgt tcatgatttc
      421 aacaaaaata ctggatattc tgtacaattc ttatcagcac catatgaacc aaatgaattt
      481 ttcaaaattg ttcatgggtt agaagaagaa cctgaaggtg gggatcgttg taaagtttgc
      541 tatgatttcc gtcttgataa gacagcggaa aaagccgttg aactaggttt tgattacttt
      601 ggcagtgccc taactattag tcctcacaaa aactctcaaa caatcaatac tattggtatt
      661 gatgtccaaa aaatttatga tactcagtat cttcctagcg accttaaaaa aaataaaggt
      721 taccaacgtt ccgttgagat gtgtaaagat tatgatatct accgacaatg ttactgtggg
      781 tgtatcttcg gtgctaaaga tcaagggata aatcttctcc aaatcaaaaa agatgcgaaa
      841 gctttcgtga gtgataaaga cggcaaagaa gaatttccta atatacgctt cacatttaat
      901 ggcaagtcaa tgtaaatgat aaaaaaagac ttagtaaaga actaagcctt tttggtataa
      961 atcttgtcaa actttaataa tacaacttct taatcttttg cagacgttgg tttagaaata
     1021 gaccacaaac aaaaatacct gtgattaaac caatccaata ggcaaacgga cctaactggc
     1081 tatttttttc taagataacc gctaatggca aagcacataa ccaataagag cccgcaccga
     1141 tcataaatgg ttttgttgta tccttatagc ctcgtaaaat cccctgtaca ggagctgcat
     1201 aagcatctgc aaactggaaa aagagactat aagttaggaa ttgagctgta atagcgacaa
     1261 agtgagggtc actattatac attgctgcta cattctcacg aaatagaaat aaaaatagta
     1321 aggttcctga tgtaatccct accgctgtta agcgtcctat cctactataa gtgtttgcgt
     1381 cctgaaagcg ctctgcccct acttcaaacg atattgtaat agctagagca gtggaaatac
     1441 ttaaaggaaa tgcatacatt aatgatgaaa aattcatagc agcctgatgt gctgcaataa
     1501 tgattgaaga aaattttgcc atgaataagc ctactactgc aaaaattgca acttctgcaa
     1561 aaatttgtaa accaatcggt aatcccaatc gaatatcttc aataatcaaa ggagctttta
     1621 ttctttccag agtccatata tgatatgttt taatttgagg atgaagtgac atcacaataa
     1681 taataacaat aaaaatagcc caataagtta aagaagttcc aagacctgcc cccgcacctc
     1741 ctagtctagg cataccaaat ttaccgtaga taagcatata attaaaaaat gaattaaagg
     1801 gtagaattaa aagcatcaga tacatagata accttgttaa ccccaatgca tcaaagaatg
     1861 aacggcaaat gctaaacaac accagcggca tgattccaat caacatataa tttaaataac
     1921 cacgaccaac tgctagaact tcatcttcta aacccaaact ccccaagaca ggttgagcaa
     1981 taaattgcat gattaaaaat aatattaagg acagtatcaa acctaaatat agaaattgat
     2041 gaaattctgt gcgaatttgt tctttatttc ctctacccaa atgttgacca actactggta
     2101 ctaatgctga aatcatacct actaataaag cgaaaaacgg agtccataaa ttactagcag
     2161 ttgacacacc tgccaaatgt agctgactat actgtccagt cataaccgaa tcaataaaag
     2221 tagctgaaaa attagcaaat tgataaatca atactgggat aaataatttc aaaaataaaa
     2281 caaatttttc cttatttgtc tgagtttgat acatcttttt tctccttttc accttaatca
     2341 atttatttta gcttagctag gatactctgt caatgctatt taaaaaggtt gagaaataat
     2401 cctcaacccc attttagcta agctcagtat tgatttgcca tgtcatacca tatttgtcag
     2461 tcacatttcc ataagctgga ctccacggaa ctgcttgtag aggcatttta atgttcattg
     2521 catctcgaga aagcttattg aataactctt gcgcttcttc tacagattcc acaattaaag
     2581 ctgctgtcat attttgtcca atttgaaaat caaaaatagg attattgtca gaaagttgca
     2641 ttcgaatacc atcaatatta aattgagcgt tcataattaa atgttcaagt tctggcggac
     2701 aatctggcca accctgtgca aaagttgtga cgtgttctgc ttttgtatta aaagcctctt
     2761 cataaaatgt aatagcttcg gccccacgac cattaatatt catataaatt tcaattgctt
     2821 tcattaccat acctcacttc atattatcca accaacgctg catcagagtt tcattttccc
     2881 cttttagggt aaaataagaa ccacgtatta tacctgcgag ttttgacttt tgacgagcaa
     2941 acttaaactt gtccgctaat tcaataggaa tatccattcc atctgataat tttaaaccta
     3001 cagctctctt tttaggatta tctaattcat ataaaatatt aatggctaaa ccatcctcat
     3061 agaaaacatg tgtccataaa atacgattct ctaaaaaaag ctgtgaaatc tctaaaggct
     3121 ttgcagcaaa aacaaggtta cgttcatttt ttagaaggtg attaatatct gccatcaatt
     3181 ctggatagtc tgatactgga aatgtttcgt aggctacttt gaatttattc cagaaatagc
     3241 gagcttcatt tgcacgcaaa ccagccaagg cttctgccac tggagaagtc tccaaacctt
     3301 tagttgaaac ggttttaaag tcgataatca tgttctttcc tttttgatta aaatcttcga
     3361 taatttagta tactcttttt tgaagaaaaa gtacattcta atcacttata aaaagtagta
     3421 catttagaac actaccacct cagtaatttc ttgacctttc tctacttaat acttataatt
     3481 aatctagact taatgtcaaa gcatcaatca aggtgtaaac ctgattcacc taaaaagctg
     3541 atggagctaa acatgaccaa aaagatattc ttatttatct gcgctactct tttagtagga
     3601 atagccttat atacaccaac aacttctatt tttaatcata acaacgttta tgctgccacc
     3661 aagaaagcaa aaattaaatt taataaaact caaaaaaaaa ttgtcaaaaa agcaagagaa
     3721 tatgctaagt ctggccatat gtctaaagat agtatcattg aaaaattaaa aaaggattct
     3781 aagaaatatc gtcaagaaga catcaacttt gtgattaata acctcaaggt tgattacaaa
     3841 aaaaatgcct tgatttctgc caaaatttat tcaaagacca tgaatttatc taaacaatct
     3901 atctttgaac agctctattc ggagtctcca gataaggcaa cccactctga taaatttaca
     3961 aaagaagaat cccaatatgc tatagaccat ctcaaagtag atttcaaaga aaatgctctt
     4021 gagactgcta aaagttatca aagtagttct tctttatcca aagaagaaat ctataaacaa
     4081 ttgacatcta cgctgggcga taaattcaca aatgatgaag cccaatatgc tgtagatcat
     4141 ttgaaatgat gacataccaa tagtctatcc ttatgtaata gaattgatag cttatgctaa
     4201 tattccagct gtaaaatagc aagaaccctc ctaagttcat taagacttag gagggttttt
     4261 atcaatatga ataggttaca ctaaactaga cagaaattat aaagtgttct acacttaaga
     4321 aaaactggag aaaaaccatg tctagaaaag tacgtcgtca cttcaccgat gattttaagc
     4381 aacaaattgt tgacctttac aatgttggta gaaagcgtag cagtctcatc aaggtatatg
     4441 agctaacccc ttccaccttc gataagtggg ttagacaagc caaaacaact ggttcattca
     4501 agtctgttga taatctgaca gatgaacagc gggagctaat tgaactcaga aaacacaata
     4561 aagaactcga aatgcaatta gatatcctaa agcaagcagc ggtgattatg gcacaaaaag
     4621 ggaaataatc actgctaata aggataaata cagcatttca aagatgtgtc gctggctgaa
     4681 tatgccacac tcaagttatt actatcaagc cgtggagtca gtatctgaaa cggagtttga
     4741 agaaactatt aaaagaattt tcctcgatag cgagtccaga tacggatcca gaaaaatcaa
     4801 aatatgcttg aataacgaag gtatcacact ttcacgtcgt cggattcgac gcattatgaa
     4861 gcgactcaat ttggtttctg tttatcagaa agccaccttc aaaccacatt ctagaggcaa
     4921 gaatgaagcc cctattccca accacttaga caggcaattt aagcaagaaa gaccactaca
     4981 ggccttagtc actgacttaa cctatgttcg tgtaggcaat cgttgggctt atgtttgcct
     5041 catcattgac ctatacaacc gtgaaatcat cggcctgtct cttggttggc acaagaccgc
     5101 tgaactcgtt aagcaagcca tacaaagcat cccttacgcc ctgaccaaag tcaagatgtt
     5161 ccattcagat cgtggcaaag agtttgataa tcagttaatt gatgaaatat tggaagcctt
     5221 tggaatcaca cgttcgctta gtcaggctgg ttgtccttat gacaatgccg tagctgaaag
     5281 tacgtatcgt gctttcaaaa ttgaatttgt ttatcaagaa acctttcaat tgctggaaga
     5341 actagctctt aagactaaag actatgttca ttggtggaat taccaccgca ttcatggtag
     5401 tctcaactac caaacaccaa tgactaaaag attaattgcc tagaaaaaca ctttataaat
     5461 gttgtacaga aaagtgttgc cttttcactt aataaaagaa agattgaagg aacacttctc
     5521 tttcattccg accaagggtc acaatttaag tccggggaat ttagaaaaat aattgatgac
     5581 aacaataggc actgaatccc taaaatgaga caaaagaaaa acactcctgt tgaaatttag
     5641 taaactagaa acaggagtgt tttattatgg ttaaaaaagc atattcatgg gaaactaagc
     5701 tggcttgcat cgatatgaag aaagcaggca agtctaacag agttattatg gagactttag
     5761 gcatcaaaaa taatagtcaa atctatactt ggatgaaatg gtacgaaaac gaggagcttt
     5821 atcgttttca tcaaggtgtt ggcaagcaat atacctacgg taaaggcttg gaacatctgt
     5881 ctgaagtgga acaattacaa ttacaggttg acctcttaaa aaagtatcga ggcttgataa
     5941 ggaaatcgat aaagtaagtc ttatcaagct tgtggaagac tataaaaaca actacccgat
     6001 agttttcatt ctagactgtt ttaaggtcaa gcggtcgacc tattaccgtt ggaaaaagga
     6061 gtatgagaag ccgcaaaaaa agatgacctt attgaattaa ttgaacagct ctgtatggag
     6121 aatcacttta tttatggcta tcgtaccatt acacgattgc tcaaaaaaat ccacggattg
     6181 actgttaata caaagaaagt ctatcgtatc atgaaaaata atggctggct ttgtcggaca
     6241 cgtaccaaga aagttcccaa tttgggtaaa gcttattatt taacagataa taaattgagt
     6301 cgggatttcc atgccgataa gcctaaggaa aagctcgtaa cagatattac ctacctatat
     6361 ttcggtaact gtaaattgta tctctcatca attatggatc tctataaccg agaaattata
     6421 gcttacacta tctcagattg tcaggacacg gattttgtac tagacaccct taatcagtta
     6481 aaattgccta aataaaggag caatcttaca cagtgaccaa ggctcagtgt atacttctat
     6541 ggcttactac caggcttgca cggaaaaaga cattatccgc tcaatgtctc gaaaaggaac
     6601 acctgcagat aatgcctgta ttgaatggtt tcacaccgtc ctaaagactg aaacctttta
     6661 tttccataat aggagaaaat acaacaaaga tagtataaca aatattgtta aaaattacat
     6721 tacattttat aatgaaacta gaattcaaca gagattaaac gaccagtcgc ctgtacagta
     6781 cagaaaactg attgcctaaa agtgtttttc ttgcgtctca ctattagggt gcagtgccaa
     6841 tatcatgcat tctttttcta atcctggata tccttatgat aatgccgtaa ctgaagcatt
     6901 tttcaagtat ttaaagcata gacaaatcaa ccgaaaacat tatcaaaata tcaaacaggt
     6961 tcaattagac tgctttgaat acattgagaa tttttataac aattacaacc cacatacggc
     7021 taatctagga ctaaccccta atcagaaaga agaaaattat tttaactcaa taaaataaca
     7081 cagttttctg tctacttatt tgacattagt ccaagaatcc tcctcttttt ttactattct
     7141 aacatggata ccatttcttt gcaaaagaaa accgctatta tccaactttt atcactactc
     7201 gttacataga atgacttcaa aatcaccgta acttgctttg acaaagcagt gctttaacaa
     7261 tgagatcttc ttttggttaa gggtttattt gttgaagtgt cttgatatca taagttacca
     7321 attcaccatt tttattataa aattggacac cttctggttg gaaaatgaga tacttaggat
     7381 cgatattagc tttttgtgct aattgattga tatgatcttc tagggttgct ctatctagac
     7441 catagtctgg taaactgtct ataaagtcat ctgattcttt ttcttcttta ctggcttcac
     7501 ttggctgttg gttttcattg ctcttatctg cctgttgctt ttcttcaggt ttatccgtat
     7561 cagtagcatc accagcattt ttctttgcca ataactcttg agcttgttgc cactcagctt
     7621 gggatagatc ggatttatta atggttctca atgaactgcc atctgctctt gggatgctaa
     7681 aggagccatc tttccataca aaagtttctt ttgccaaaac atctcgtgga tcgaaaatat
     7741 agccgtctgg tgctgcaaaa cgaccttctg ctagggcttt ttgaacttct tcagcagaat
     7801 ggataatttg ccagtttggc ataccagcac gtttatcaag aggcgtaaca tttggaatga
     7861 ccgaacctga ctcttcatgc cctggcttag accatacatc cggacgaact tcggggtgtt
     7921 gcatcacata cttgattgtt gcaatctgat tgcgcgtcaa ccatgaatac ggaacgacat
     7981 ggatatgatc aatatgtggg ataacaaacg aacttccagt atcgtaagta gcattaccag
     8041 catgcatcgg cagacttgac acatctacag caagtcgtgg ctcaatgcct gtatcaacaa
     8101 tgtcataacg gtaatgcttc ttatctttaa gcattagttc ttgttcggca aaggcaatct
     8161 gagtcaaatc aagttgataa cgagcataga aatagtcctt gccatctttt ggcataatat
     8221 agcccacttt accatctttt gttactttgc gactcacttt tttagtgtca aagagtggtt
     8281 tttctttgcc ttgttcctga tccaaagcag caacaagctc atcagcttga ccttttgctt
     8341 tcacccagtt agcgacctca tccaactcat attgttcaag ttctccaaat cctatatagt
     8401 ggaaatgatc tccgtgttta gctgtaactc ctgatttatc cactgaatga atggattctt
     8461 tactaaaaac ataagcatca ctcgtatcat atggtttacc atctaagcct tttccgtaag
     8521 ctttgatgcg atgaccaaga aaggtatgtg tcacttcttt atctgatggt tttgagtgat
     8581 ctgaacctga gtcgttgtca tcagtttggc cggctaagta tcgatctgct aattccattt
     8641 caagaggtga taactgactt cttgggataa tatgataatg atctccatga ggcaccacat
     8701 acccaaaagc gtttgatttg atcacttgag tcggttcaaa aatcaaccca tcttcttcca
     8761 catgacggta tttcaaatca agacggtgta gttgatctaa aagttcctta aaggtttttc
     8821 ctttaaactc atctctttgg tgtttgtttt gtgacgcatc atttggccta ggaggcgctg
     8881 gatgataacc accgttatct ggctgatgac cttgtccagg gttaggcgtc acatcaggaa
     8941 ttggggcttt cctacgacct ggggctggtg tcgggcggta atcagacggt ctagcacctc
     9001 gaccttgttt ttgactccag taggcttgtg cagcagctag ctcacttgga gacaaatctt
     9061 ttttaggaat ataatgatag tgattaccat gaggtactaa ataagcatct cctaaatcat
     9121 caatgatatc tgtcggacta aaaatatagc catcgtctgt agtatagcgt ccttgtcttt
     9181 ttgcttcatt gactgccgca acttcttctt tactgagatg ggccacttga gctaaacctt
     9241 tttctttagc ttctttagtt cctttggcta cttgctcagc aatttgttgt ttggttcgaa
     9301 tgtttttgcg cttactacct ggcttgaggt aaacataata gttgccattg actttaataa
     9361 cgtaaccgtc taagatttca ttgataacgt ctgattgttt aaaatggtaa ttaggatccg
     9421 tcatcaacaa ctcttcacta ataatcgcat cataaggaac tttcccattg taaaaatgat
     9481 aatggtcacc gtgtgaggta acataacctt ggtcagtaat tttgactacg atctgttcag
     9541 cagagatgcc ttcttcagca ctgatttgat ccatcgtttt gtttgtttta ggggctttta
     9601 ccttaccttt gctatcatca atataggcaa tctgattgtc ctttgttgct agacccatat
     9661 gatgcttacc aagctggtaa cttccaatat gagtagctag taaaatagca gcaactgagc
     9721 cgatataacc atatgttttc ttcactaata atctccttta cttcaactgt tgatagagca
     9781 cttccaaatt tgctctaaga ttttctagat atgtcttgtt tccgcttgga gcagcttcaa
     9841 gtggacttaa tgtctttact ttagctcctg ttgatttcgc aatagcatga gcaattttgg
     9901 ggttgacgtt gtcttctgca aaaatagtct tgacgttgta ttctttaaca aagtcttgaa
     9961 tttctttcaa ttggcgagga gagggctctt gctctggaga aatacccgag ataccaagtt
    10021 gtttcaagcc gaatcgttta gccagataag aaaatgccgt gtgttgcgtc acaaatgttt
    10081 ttgagcgcac ctttttaaat ttttgagtgt attcttcagt tagttgctct gcttcttttt
    10141 tgaaagcctt agccttttta gtgtaactgt ctttgtgttt aggatccaaa tgtcctagct
    10201 ctttagcgat attaacagct tcctcaccag ctaaaacggg atccgtccag gtatgtgggt
    10261 cataaagtgt cgcagggtca atgccttgtg tgacttccat atcttctagc cctttgactc
    10321 tatctagtgt cagaggtttt gacgcttcaa acacattaac ctttgatttt tttaaattag
    10381 ggtctagatc ccttgcccaa gcttctaagg tatgtgaatg gtaaacaaac aaatccgcgt
    10441 cataaatagc tgccacatca tttacagacg gttcaaagga atgaatgcct gcacctgatt
    10501 ggatcatcct cacatcattt aggtctccag atacttcttt tgtcatcgca tacattgggt
    10561 aaaagctggt tacaactgac atgccttgcg taggctgttt ggggtttgct gacttatcac
    10621 accctgctat cattactaaa ctcacaacca tagccatgag aaaaaaaact tttttcatag
    10681 tacctcctca attataattt aaccagttat taaccagtta attttactcc tttatcaatg
    10741 ctttgtcaat gacaactacc atcttttatc acatcaactg aagggataag caaaaggcaa
    10801 ttgttgacag ctatttgtcc taacttcttt ttgatgaggt agca
//
LOCUS       DATROD010000059        10809 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.059, whole genome shotgun sequence.
ACCESSION   DATROD010000059 DATROD010000000
VERSION     DATROD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10809
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.059"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..190
                     /locus_tag="VBC74_001344"
     CDS             <1..190
                     /locus_tag="VBC74_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942288.1"
                     /translation="SKSAMYNILTFIIYYMGYKLFTFTKSITMPILLVSLGIIVVILI
                     IGYLAVVVFAPKTFRLKS"
     gene            386..1555
                     /locus_tag="VBC74_001345"
     CDS             386..1555
                     /locus_tag="VBC74_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEO6942289.1"
                     /translation="MAWLGNFFTGASFSLVMPFMVLYVEQLGAPSNKVEWYAGLSVSL
                     SALSSALVAPLWGRLADKYGRKPMMVRAGLMMTFTMGGLAFIHSVTGLLILRILNGIF
                     AGYVPNSTALIASQAPQEESGYALGTLATGVTGGMLIGPLLGGLLAEWFGIREVFLLV
                     GTILLISTLMTIFMVKEDFKPISNEETMPTTEVFKSVKSLQILIGLFVTSMIIQISAQ
                     SIAPILTLYIRHLGQTENLMFVSGLIVSGMGFSSILSSPKLGRIGDRIGNHRLLLLAL
                     LYSFLMYVLCSLAQTSLQLGVIRFLYGFGTGALMPSINSILTKIAPRQGLSRIFSYNQ
                     MFSNLGQVLGPFVGSAVSIHLGFRWVFFVTSFIVLANFVWCFINFRKYIRVKEIV"
     gene            1552..1698
                     /gene="rpmG"
                     /locus_tag="VBC74_001346"
     CDS             1552..1698
                     /gene="rpmG"
                     /locus_tag="VBC74_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEO6942290.1"
                     /translation="MRVKINLKCSSCGSKNYLTSKNKSNHPDKIEVPKYCPKERKVTL
                     HIES"
     gene            1745..1981
                     /gene="secG"
                     /locus_tag="VBC74_001347"
     CDS             1745..1981
                     /gene="secG"
                     /locus_tag="VBC74_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEO6942291.1"
                     /translation="MYNLLLTILLVLSVLLIISIFMQPQKNPSSNVFDSSGSEALFER
                     SKARGFEAFMQRFTGVLVFFWLLIGLVLSILSSH"
     gene            2094..4499
                     /gene="rnr"
                     /locus_tag="VBC74_001348"
     CDS             2094..4499
                     /gene="rnr"
                     /locus_tag="VBC74_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEO6942292.1"
                     /translation="MKEKIIKYLEEHGKTSVNDLAAALDMAGAKAFPKLIKTISNLES
                     HRQLRFDDNGSLSLQKKEAKKKEITVRGLFRANKAGFGFLSIDQDEDDMFIGKNDIAY
                     AIDGDTVEAVVKKPADRLNGTAAEARVVNIVERSLKTLVGKFVLDDERPKYAGYIKSK
                     NQKINQKIYIRKEPVVLDGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVLE
                     VLESMDIVSEFPEDVIAEANAIPDAPTEKDLIGRVDLRQEVTFTIDGADAKDLDDAVH
                     IKLLDNGHFELGVHIADVSYYVTEGSALNREALSRGTSVYVTDRVVPMLPERLSNGIC
                     SLNPNLDRLTQSCIMEIDQNGRVVNHQITQSVINTTYRMTYIAVNDIIAGDEEICSEY
                     ESIVSSVQHMVTLHHTLEAMRTRRGALNFDTSEAKIMVNDKGMPVDIVIRNRGIAERM
                     IESFMLAANETVAEHYARLKLPFIYRIHEEPKAEKLQKFIDYASVFGVQIQGTATKIT
                     QSALQDFMKKVQGQPGSEVLSMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRRY
                     PDLLVHRMIRDYDDKAMDKADHFANLIPEIATQTSSLERRAIDAERIVEAMKKAEYME
                     EYVGEEFEGVVASVVKFGMFVELPNTIEGLIHVTTLPEYYHFNERTLTLQGEKSGKVF
                     RVGQQIKVKLIRSDKETGDIDFDYLPSDFDIVEKVSKSSREGRPNRSSKREHQHRISD
                     RDNKNKNTSKKKASRKPKRNSDSKSHHHKDDRTTGSTKKKTKKPFYKGVAKKGQKRKS
                     KKG"
     gene            4502..4969
                     /gene="smpB"
                     /locus_tag="VBC74_001349"
     CDS             4502..4969
                     /gene="smpB"
                     /locus_tag="VBC74_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEO6942293.1"
                     /translation="MVKGQGNVVAQNKKAHHDYTIVETIEAGIVLTGTEIKSVRAARI
                     TLKDGYAQIKNGEAWLINVHITPYDQGNIWNQDPDRTRKLLLKKREIEKISNELKGTG
                     MTLVPLKVYLKDGFAKVLLGLAKGKHDYDKRESIKRREQNRDIARQLKNYNSR"
     gene            complement(5053..6441)
                     /locus_tag="VBC74_001350"
     CDS             complement(5053..6441)
                     /locus_tag="VBC74_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO6942294.1"
                     /translation="MSKNNNDHTQKSENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SISLTGPSIVLVYAITGAFMFFMMRAIGEMLYQDPNQHTFINFITKYLGPGWGFFSTL
                     SYWISLIFIGMAEITAVGEYVQFWFPEWPSWIIQIVFLAILSSINLIAVKAFGETEFW
                     FAMIKVIAILGLIATGIFMVLTNFDTGHGYHASISNITNHFEWFPKGKLNFFMAFQMV
                     FFAYLAIEFVGVTTSETANPRKVLPKAIQEIPMRIILFYAGSLLAIMAIFPWQQLPVN
                     ESPFVTVFKLAGIKWAAALINFVVLTSAASALNSTLYSTGRHLFQLANESPNALTKAL
                     KLDQLSRQSVPSRAIIASAVIVGASALISVLPGISDAFSLITASSSGVYISIYVLIMI
                     AHWKYRKSPDFMEDGYKMPAYKILSPITLLFFLFVFVSLFLQDSTYIGAIGATIWIIG
                     FGLYSHFKHKSH"
     gene            complement(6570..7496)
                     /locus_tag="VBC74_001351"
     CDS             complement(6570..7496)
                     /locus_tag="VBC74_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6942295.1"
                     /translation="MALLSVIVPCYNEQETVSTFLTEIKKVESEMARYTHFEYIFVND
                     GSTDRTLELLKKAAKQFDNVHYLSFSRHFGKDAALLAGLEHTTGDFITVMDVDLQDPP
                     TLLPEMYLKLQEGYDIVATRRKDRKGEPLIRSLFAKLFYKLINQVSDTKMVDGARDFR
                     LMTKQVVDSILELNEVNRFSKGIFSWIGYDVAYISYENRERIAGKTSWSFFNLLKYSL
                     DGFINFSEIPLAIATWIGTLSSVLSLLAIIFIIIRKLLFGDPVSGWASTVTIVLFMGG
                     IQLLSLGIIGKYISKIFLETKKRPVYIVKEEG"
     gene            complement(7625..8017)
                     /gene="gloA"
                     /locus_tag="VBC74_001352"
     CDS             complement(7625..8017)
                     /gene="gloA"
                     /locus_tag="VBC74_001352"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEO6942296.1"
                     /translation="MPFLHTCIRVKDLDASIAFYQEALGFKEVRRNDFPENQFTLVYM
                     SLEDDPSYELELTYNYDHEAYDLGNGYGHIAVGVDDLETTYDAHQKAGYSVTKISGLP
                     GKPNMFYFIQDPDGYKIEVIRLSQFKAD"
     gene            complement(8105..8779)
                     /locus_tag="VBC74_001353"
     CDS             complement(8105..8779)
                     /locus_tag="VBC74_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEO6942297.1"
                     /translation="MTKEDIKKQVRRAFDFRMAIRVYNNNDIPKEDMEYILDTAWLSP
                     SSVGLEGWRFLVLDRQTIAKFRDKLKEVAWGAQYQLDTASHFVLLLAEKGAYYNADSM
                     INSLIRRGLGDPAALESRIPLYKSFQENDMKIDSERSLWDWTAKQTYIALGNMMTAAA
                     MIGVDSCPIEGFDYEKVNNILSKEGLIDDKKEAISCMVSFGYRLREPKHSRARKERQE
                     VITWVE"
     gene            9046..9888
                     /locus_tag="VBC74_001354"
     CDS             9046..9888
                     /locus_tag="VBC74_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6942298.1"
                     /translation="METYTLSNTLNIPKIGFGTWQLTEGEEAYKAVTHALKVGYTHID
                     TAQIYGNEHSVGRAIRDSGLARESIFLTTKIWNDKHDYHLAKASIDESLQKLGVDYID
                     LLLIHWPNPKALRENDAWKAGNAGTWKAMEEAYKEGKVKAIGVSNFMKHHLEALFETA
                     EIKPMVNQIILAPGCAQEDLVRFCKGNDILLEAYSPFGTGTIFENESIKAIAEKYGKS
                     VAQVALRWSLDNGFLPLPKSATPKNIEANLDIFDFQLNEDDIATLIQLDSGIKPKDPD
                     NVSF"
     gene            10079..10801
                     /locus_tag="VBC74_001355"
     CDS             10079..10801
                     /locus_tag="VBC74_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEO6942299.1"
                     /translation="MRLDKFLVECGLGSRTQVKLILKKKQISVNSNSETSPKVQVDEY
                     RDEIKYNGTLVSYEKFVYYMLHKPKGVISATDDPSHKTVLDLLDKTARDKAVFPVGRL
                     DIDTTGLLLITNNGELAHKMLSPKKHVDKCYEAKISGIMTEDDILAFDKGIILKDFTC
                     LPALLEIVEVNQVKKQSLVKITIKEGKFHQVKRMVAACGKEVLELKRLSMGNLQLDKQ
                     LESGQWRRLTIKEIEKLEKHMQ"
BASE COUNT         3694 a         1715 c         2086 g         3314 t
ORIGIN      
        1 atcaaagagt gctatgtata atattttaac ctttattata tactatatgg gatataagct
       61 atttaccttt actaaatcta taacgatgcc aatattatta gtaagcctag gaattatagt
      121 tgttatttta attattggct atttagcagt tgtagtattt gctccaaaaa ctttccgttt
      181 aaaaagttaa ttttaattgt ggtataataa cagaggcatg tgataacata ggtttagtta
      241 cataaaggaa attataatgg aaataacatc tcaaatagat gttaaatctc aataaaactg
      301 ctaagatagt tagtggtaga aaagtttaga aagaagatga tgttttatct cagttgaaac
      361 ggttaactgg aagcaaaatt taaaagtggc ttggctcggt aattttttta ctggggcaag
      421 cttttcactt gttatgcctt ttatggtatt atatgtagaa caattaggag cacctagcaa
      481 caaagtagag tggtatgcag gcctatcagt atccttatca gccttgtctt cagctttagt
      541 tgccccatta tgggggcgct tagctgataa atatggtaga aaaccgatga tggtaagagc
      601 agggttgatg atgactttta ctatgggagg ccttgctttc attcatagtg ttacaggact
      661 tctgatttta cgtatattaa acggcatttt tgcaggctat gtacctaact ctacagcttt
      721 gatagcaagt caagctcctc aagaggaatc aggatatgcc ttaggaacct tagcgacagg
      781 agtgactgga ggtatgttaa ttggtccact tttaggggga cttttagcag agtggtttgg
      841 tattagggaa gtttttttat tagtgggaac catattgtta atatctactt taatgaccat
      901 ttttatggta aaggaagatt tcaaacccat ttctaacgaa gaaaccatgc caactacaga
      961 agtgttcaaa agcgttaaaa gcttacaaat tttaataggg ctgtttgtaa caagcatgat
     1021 tattcaaatt tctgcacaat caattgcacc tattttaaca ctttacattc gtcatttagg
     1081 acagactgaa aacttgatgt ttgtttccgg attaattgtt tctggtatgg gattctctag
     1141 tattctgagt agtccaaagc taggaagaat cggggatcgc ataggcaatc atcgtttgct
     1201 attattagct ctactgtata gtttcctcat gtatgtctta tgttcattgg cacagacttc
     1261 attacagcta ggggttatta gattcctgta tggttttgga acaggtgcct tgatgccgag
     1321 tattaattca atcttgacta aaattgctcc aagacaaggt ttatcaagga ttttcagtta
     1381 taaccaaatg tttagcaatt taggacaagt attaggtcct tttgtcggtt cagcagtttc
     1441 tattcattta ggattccgat gggttttctt tgtgactagc ttcatcgttt tagctaattt
     1501 tgtttggtgt tttattaatt ttagaaaata tattagagtt aaggaaattg tgtgagagtt
     1561 aaaattaatt taaagtgtag ctcttgtggt agtaagaatt atttaaccag taagaataaa
     1621 agtaatcatc ccgataaaat cgaggttcca aagtattgcc caaaggagag aaaagtaaca
     1681 ctacatattg aatcttaata agttttatgt tagaatgtac tttgaccata gtggaggaat
     1741 taacatgtat aacctattac tcaccattct acttgtttta tcagtattat tgattatctc
     1801 aattttcatg caaccacaaa agaatccaag tagtaatgtt tttgatagta gcggttctga
     1861 agcccttttt gaacgtagta aagctcgagg atttgaagct tttatgcaac gttttacagg
     1921 tgttttagtc tttttctggc tactaatcgg cttagtatta tctattttat caagtcatta
     1981 atgatagcta aatacagata agtgaggatg ggggatagcc caacctcact ttttgttagt
     2041 agaatggttg tagagtaata ataaagtgac taaagttact agaaaaggaa ataatgaaag
     2101 aaaaaattat aaaatatttg gaagaacacg gtaaaaccag cgttaatgat ttagctgctg
     2161 ccctagatat ggcaggcgca aaagcatttc caaaattaat caaaacaatc tcaaatttgg
     2221 agagccacag gcaattaaga tttgacgata atggaagtct ttctcttcaa aagaaagaag
     2281 ctaagaaaaa ggaaatcaca gtaaggggtc tttttagagc aaataaagca ggatttggtt
     2341 ttcttagtat tgaccaagac gaagatgata tgtttatcgg aaaaaacgat attgcttatg
     2401 ctattgatgg tgacacggta gaagctgttg taaagaaacc agcggaccgt cttaacggga
     2461 ctgcagcaga agcgcgtgtt gttaatattg ttgaacgtag tttaaagacg cttgttggta
     2521 agtttgtctt agacgatgag cgccctaaat acgcaggata tatcaaatcg aaaaatcaaa
     2581 aaatcaatca aaagatttat atacgcaaag aaccggtcgt tttagacgga acagaaatta
     2641 tcaaagtaga tattgataaa tatccgacac gaggacatga ttacttcgta gcaagtgttc
     2701 gtgatatcgt tggacatcaa ggagatgttg gtattgatgt tttagaagtc ttggaaagta
     2761 tggatatcgt ctcagaattt cctgaggacg ttattgcaga agccaatgct atcccagatg
     2821 ctccaacgga gaaagattta attgggcgtg ttgatttacg tcaagaggtc actttcacta
     2881 ttgatggagc ggacgccaaa gatttagacg atgctgtgca tattaaatta ctggataatg
     2941 gtcattttga acttggagtt catatcgcag atgtatcata ctacgttact gaaggctctg
     3001 ccttaaatcg tgaagcactt agtcgtggta cgtccgtata tgtcacagat cgcgttgtac
     3061 caatgttacc agagcgtcta tctaacggta tatgttcatt aaatccaaat cttgatcgtt
     3121 taacgcagtc ttgtattatg gaaattgacc aaaatgggag agttgttaat catcaaatta
     3181 cacagtcagt gattaatacc acttatcgta tgacttatat cgctgtaaat gatattattg
     3241 ctggcgatga ggaaatctgc tcagaatatg agtctattgt gtcctcagta cagcatatgg
     3301 tcactttaca ccatactttg gaagctatgc gtacgcgtcg tggagcgctg aattttgaca
     3361 catctgaagc aaaaattatg gttaatgata agggaatgcc agttgatatt gttattcgca
     3421 acagaggcat tgctgaacgc atgattgagt catttatgtt ggcggcaaat gaaacggttg
     3481 ctgaacatta tgctcgtcta aaactgccat ttatttatcg tatacacgaa gagcctaaag
     3541 ctgaaaaatt acaaaaattc atcgattatg cttctgtttt cggtgttcag attcaaggaa
     3601 cagcaactaa aatcacccaa tctgctttac aggactttat gaaaaaagta caagggcagc
     3661 caggaagtga agtgttatct atgatgttat tgcgctcgat gcaacaagct agatattctg
     3721 aacacaacca tggacattat ggacttgctg cggaatatta cactcacttt actagcccca
     3781 ttcgacgtta tccagacttg ttagttcatc gtatgattcg tgattatgat gataaagcga
     3841 tggataaagc tgatcatttt gctaacctta tcccagaaat tgcaactcaa acgtctagtc
     3901 ttgagagacg tgccattgat gctgaacgta ttgtagaggc aatgaagaaa gccgaatata
     3961 tggaagaata tgttggtgag gaatttgaag gtgtggttgc aagcgttgtt aaatttggta
     4021 tgtttgtaga attaccaaat actattgaag gattgattca cgtaacgacc ttgcctgaat
     4081 attatcactt taatgaacgt acgctgacgc ttcaaggtga gaaatctggg aaagttttcc
     4141 gtgttggtca acaaattaag gttaagttga ttcgttctga taaggaaact ggtgatatcg
     4201 attttgatta tttaccatca gattttgata ttgttgaaaa ggtaagtaaa agctcgcgtg
     4261 agggtcgtcc taatagaagt tctaaacgag aacatcagca tagaatttct gatcgtgata
     4321 ataagaataa aaatacatct aagaaaaaag catctcgaaa acccaaaagg aactcggata
     4381 gcaaatcaca tcatcataaa gacgatagaa caacaggttc aaccaagaaa aaaacgaaaa
     4441 aacctttcta taaaggcgtt gctaagaaag gtcagaaacg gaaatctaag aaaggttaag
     4501 tatggttaaa ggtcaaggaa acgttgtagc acaaaataaa aaagcgcatc acgactatac
     4561 tatcgttgaa acaatagaag caggaattgt tttgacagga acagaaatca aaagtgtccg
     4621 cgcagcgcgt attacattga aagatggata tgcacaaata aaaaatggtg aagcgtggtt
     4681 gattaatgtt catattacac catatgatca aggtaacata tggaatcaag atcctgatcg
     4741 tacacgaaag ttattactaa aaaaacgtga gattgaaaaa atatcaaatg aactgaaagg
     4801 gactggtatg actctggtac ctctaaaagt ttacttaaaa gatggttttg ctaaagttct
     4861 attgggtttg gcaaaaggaa aacatgatta tgataagcga gagtctatca aacgtcgtga
     4921 gcaaaaccgt gatattgccc gtcagttaaa aaactataat tcacgatgat tatcaaaagg
     4981 ttagggaatt gcctaacctt ttttggacga aaaaagtgaa gttgattgac caacttcact
     5041 aataatatag acttaatgtg atttatgttt gaagtgacta tataaaccaa atccaataat
     5101 ccagatggtt gctccaatag ccccgatgta tgttgaatct tgcaagaaga gagaaacgaa
     5161 tacaaagagg aagaagagaa gtgtgattgg tgaaaggatt ttgtaggcag gcattttata
     5221 accatcttcc ataaaatcag gtgatttacg atatttccaa tgtgcaatca taataagaac
     5281 atagatagaa atgtaaacac cagaagatga tgcagtaatt aaggagaagg catctgaaat
     5341 accaggtaaa acactgataa gtgctgaagc tccaacgatt acagctgatg caataatagc
     5401 tcgacttgga acgctttgac gagataattg gtctaatttt aacgcttttg taagagcatt
     5461 tggtgattca ttagccaatt ggaataaatg acgccctgtt gagtacaaag tagaattaag
     5521 agcagatgct gcagacgtta ggacaacaaa gttaatcaaa gcggcagccc acttaatccc
     5581 agctagtttg aatactgtaa cgaaaggtga ctcattaact ggtaactgtt gccatgggaa
     5641 gatggccata attgctaata atgaacctgc gtagaataaa ataatacgca tcggaatttc
     5701 ttggattgct tttggtaaaa ctttacgtgg attagctgtt tcagaagttg ttactccgac
     5761 aaattcaata gctaaataag caaagaatac catttggaaa gccatgaaga aattcaattt
     5821 acctttaggg aaccattcaa aatgattggt gatatttgag attgaagcat ggtatccgtg
     5881 accggtgtca aagtttgtca ataccataaa gataccggtt gctattaaac caagaattgc
     5941 aataactttt atcatagcaa accaaaactc ggtttctccg aatgctttta cagcaattaa
     6001 gttgatagaa cttaggatag ctaaaaaaac aatttggata atccaggatg gccattcagg
     6061 gaaccaaaat tgaacatact ctcctacggc tgtaatctct gccataccga taaatattag
     6121 ggaaatccaa taagaaagtg ttgagaaaaa tccccaaccg ggtcctaaat atttggtaat
     6181 gaaattaata aaagtatgct gatttggatc ttgataaagc atctctccaa tagcacgcat
     6241 catgaagaac atgaaagcgc cagtgatagc atagacaaga actattgaag gacccgtcag
     6301 gctaattgaa cgacctgcac caagaaaaag cccagttccg attgtccctg caatcgcaat
     6361 taactgtacg tgacgatttt ctaggccgcg taccatacca ttctcagatt tttgagtgtg
     6421 gtcattatta ttttttgaca ttataaatac tccatctatt tttgaaaaac tccttcatta
     6481 tataattttt ttgcaaaatt cgcaataatt actatacaat atttttctat gataaaatct
     6541 cctagctaag tgagctaaga gatttcaatt catccctctt ccttgacaat atagactggg
     6601 cgttttttag tttctaggaa aatcttagag atgtatttgc ctataattcc tagcgacaat
     6661 aactgaatac cacccataaa aagtacaatg gtaactgtgc ttgcccaacc tgaaacggga
     6721 tccccaaata gtaattttcg aattataata aaaatgatag ctaaaagtga tagtactgaa
     6781 ctaagggttc caatccaggt agctatagct aatggaattt ctgagaagtt gatgaaaccg
     6841 tctagagagt attttaagag attaaagaaa ctccatgagg tcttacccgc aattcgttct
     6901 cgattctcat aactaatata agcgacatca tatccaatcc aggaaaaaat cccttttgag
     6961 aagcgattca cttcatttag ctcgagaata ctgtcaacaa cttgttttgt catgagacga
     7021 aaatcacgcg caccatcaac cattttagta tctgacactt gattgattaa tttataaaaa
     7081 agcttggcaa aaagagagcg tatcaacggc tcccctttac gatcttttcg ccttgtagcg
     7141 acaatatcat aaccttcttg aagttttagg tacatttctg gtaataaagt gggtggatct
     7201 tgcaagtcaa catccataac agtgataaaa tcaccagtag tatgttctaa accagccaat
     7261 aaagcagcat ctttaccaaa atggcgtgaa aatgaaaggt aatgaacatt gtcaaattgt
     7321 ttagctgctt tcttaagaag ttcgagtgta cgatcggtag aaccatcgtt tacgaaaata
     7381 tattcaaaat gggtgtaacg agccatttcg gattcaactt ttttgatttc tgttaaaaaa
     7441 gttgagacag tctcttgttc attataacaa gggacaataa ctgataataa agccatttgt
     7501 aattctccgt atcattcttg cttaaatttt aacataatat gggaaaaaat agaaagatta
     7561 taaggtaatc ataaaaagga ctaggttttt gcctcagtcc ttaacttaat atgatgagat
     7621 gttattagtc agctttaaat tgactgagtc gaataacttc aattttatag ccatctggat
     7681 cttgaataaa gtagaacata ttaggtttac ctggtaatcc agaaattttt gttacagagt
     7741 aacctgcttt ctgatgggca tcataagtag tttccaaatc gtctacacca acagcaatgt
     7801 gaccataccc atttcctaaa tcataagctt cgtgatcata gttataggtt aactctaatt
     7861 cgtaactagg gtcgtcctct agggacatat agactaaagt aaattgattt tcaggaaagt
     7921 cattacgacg aacttcctta aaaccaagtg cttcttgata aaaagcaatt gaggcgtcca
     7981 aatctttaac gcgaatacaa gtatgtaaaa atggcatagt ctctctccta tttctttatt
     8041 aaacgtatta tgtcaaaata aaattatctt ttcaaaaata ttgattataa attaggctgt
     8101 ctatttactc aacccaagta atgacctctt gacgttcttt cctagcacgg gaatgtttag
     8161 gttcacgcaa acgataacca aatgaaacca tacaagaaat agcttctttc ttatcgtcta
     8221 ttaacccttc tttactaaga atattattga ccttttcata gtcaaatcct tcaattgggc
     8281 atgaatctac gccgatcata gcagcagccg tcatcatatt accaagtgcg atataagttt
     8341 gttttgctgt ccaatcccaa agagaacgtt cagaatcaat tttcatatcg ttttcttgga
     8401 aggatttgta gagaggtatc ctactctcaa gggcggctgg atcacctaga ccacgtcgaa
     8461 tgagactgtt aatcatgcta tcagcgttat aataagcacc tttttcagct agtagtaaaa
     8521 caaagtgact agcagtatct agttgatatt gtgctcccca agcaacttcc ttaagtttat
     8581 ctcgaaattt tgcaattgtt tgtctatcta gtactaaaaa tcgccagcct tctaaaccga
     8641 ctgaagaagg actcaaccaa gcagtgtcta aaatatactc catatcttct ttaggaatat
     8701 cattattatt ataaacacga atcgccatac gaaaatcaaa agctcttcga acttgttttt
     8761 tgatatcttc ttttgtcatt ttagatcctc ctgttattaa tgctatcata tcacaattta
     8821 gaaaaaatct cagcaggcgg tatcgtggca aagtagagct acaaaattct aatgactgtc
     8881 agaaaaataa agggaatgaa caaaatgata gggttttgat gaagaaagtt atataatttt
     8941 acataaggag gggataatac catatatcaa actatcattt agatgtttaa aatagatgtt
     9001 tttcactatg aaattcattt ttgtcacata gaaaaggaga ttgttatgga aacatatact
     9061 ctatcgaata ctcttaacat tcctaaaatt ggttttggga catggcaact aactgaaggt
     9121 gaagaagctt ataaagcagt gactcatgct ttaaaagttg gttatactca tattgatact
     9181 gctcagattt atggtaatga acatagtgtt ggacgtgcta taagagacag tggtttggcg
     9241 cgagaaagta tctttttgac aactaagatt tggaatgaca aacatgacta tcatttagcc
     9301 aaagcttcta ttgatgaatc tctgcagaaa ttaggtgttg attatattga tcttcttttg
     9361 attcattggc caaatccaaa agctcttcgt gaaaatgatg cgtggaaagc tgggaatgca
     9421 ggcacttgga aagcgatgga agaagcttat aaagaaggaa aggtaaaagc tattggtgtt
     9481 tcgaatttca tgaaacatca cttagaggct ttatttgaaa ccgccgaaat aaaacctatg
     9541 gttaatcaaa ttatcctagc accaggttgt gctcaagaag atttggttcg cttctgtaaa
     9601 ggaaatgata tcttattaga agcttacagt ccatttggaa caggtactat ttttgaaaat
     9661 gaatctataa aagctattgc tgaaaaatat ggtaaatcag ttgcacaagt agctcttcgc
     9721 tggtcacttg ataatggctt tttaccatta cctaagtcag cgactcccaa aaatattgaa
     9781 gcaaaccttg atatttttga ttttcaatta aacgaagatg atattgctac cttaatacaa
     9841 ctagattcag gtattaaacc caaagatcca gataatgtct cattttaata agaaaagatg
     9901 gaaatgtatt tccatctttt ttatgtggga tagttcctta aagttaagaa aaacattaag
     9961 acctccattg acgaggagaa tatattaatg atagtattac tagaaaagta gtaataaagg
    10021 atgagtatgg taaaatcctt tacaatatat cattatcgaa agaggttata acatctaaat
    10081 gagacttgat aaatttttag tagaatgcgg acttggttca cgtactcaag tcaaattaat
    10141 attgaaaaaa aagcagatta gtgttaatag taatagcgag acatctccaa aagtacaggt
    10201 agatgaatat agagatgaga ttaaatataa tggaactcta gtgtcatatg aaaagtttgt
    10261 ttattatatg cttcataaac ccaaaggtgt tatatcagca acggatgatc cttcgcataa
    10321 aaccgtatta gatttgctag acaaaactgc tcgagataaa gctgtttttc cagtggggcg
    10381 tttagatatt gatacaacag gccttttgtt aataactaat aatggggaat tagcgcataa
    10441 aatgttatca cctaaaaaac acgttgataa atgctatgag gcaaaaatct cagggataat
    10501 gacagaagac gatattttgg catttgataa agggattatt ctgaaagatt ttacttgttt
    10561 accagcatta ttagaaattg tggaagtaaa tcaggtgaag aagcagagtc ttgttaaaat
    10621 cactattaag gaaggaaaat tccatcaagt taaacgtatg gttgctgcgt gtggcaagga
    10681 agtcttagag ctaaagcgtc taagtatggg aaatctacaa ttagataaac agctagaaag
    10741 tggacaatgg agacggctaa caataaaaga aatagaaaaa ttagaaaaac atatgcaata
    10801 atataaaat
//
LOCUS       DATROD010000060        10045 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.060, whole genome shotgun sequence.
ACCESSION   DATROD010000060 DATROD010000000
VERSION     DATROD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10045
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.060"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            223..1104
                     /locus_tag="VBC74_001356"
     CDS             223..1104
                     /locus_tag="VBC74_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEO6942300.1"
                     /translation="MKKHKMLSLLAVSGLMGIGILAGCSNDSSSSSKGTINIVSREEG
                     SGTRGAFIELFGIESKNKKGEKVDHTSDAATVTNSTSVMLTTVSKDPSAIGYSSLGSL
                     NSSVKVLKIDGKNATVKDIKSGSYKISRPFNIVTKEGKEKEATKDFIDYILSKDGQAV
                     VEKNGYIPLDNAKAYQAKVSSGKVVIAGSSSVTPVMEKIKEAYHKVNTKVDVEIQQSD
                     SSTGITSAIDGSADIGMASRELDKTESSKGVKATVIATDGIAVVVNKKNKVNDLSTKQ
                     VKDIFTGKTTSWSDLSK"
     gene            1119..1964
                     /gene="pstC"
                     /locus_tag="VBC74_001357"
     CDS             1119..1964
                     /gene="pstC"
                     /locus_tag="VBC74_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEO6942301.1"
                     /translation="MKQTKEGLMQAVFFITACVSVISAILICLFLFSSGLPAITKIGW
                     GNFIFGKVWHPSNNIFGIFPMIVGSLYVTAGALLLGGPIGILTAVFMAYFCPENIYRP
                     LKSAINLMAGIPSVVYGFFGLVVIVPMIRQYIGGFGMGVLAASILLGIMILPTIVSIS
                     ESSLRAVPESYYEGGIALGASHERSVFFAVLPAAKRGILASVVLGIGRAIGETMAVIM
                     VAGNQAVLPQSLTSGVRTLTTNIVMEMGYSSGLHRQALIGTAVVLFIFILMINISFSA
                     LQKEE"
     gene            1966..2844
                     /gene="pstA"
                     /locus_tag="VBC74_001358"
     CDS             1966..2844
                     /gene="pstA"
                     /locus_tag="VBC74_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEO6942302.1"
                     /translation="MENINQRTSKQYLASLKKDPLSFFLFAIVYLGAILSFATIAFVV
                     IYILVKGLPHVNTGLFAWTYNTQNVSLLPAFINTIFIIALTLLFAVPLGIGGSIYLTE
                     YARRDNPYLKIIRVATETLAGIPSIIYGLFGALFFVKYTHLGLSLISGSLTLSIMILP
                     LIMRTTEEALLSVPDSYREGAFALGAGKLRTIFKIVLPSAMSGIFAGIILAIGRIIGE
                     SAALIFTAGTVAKVVHSVFSSSRTLAVHMYAISGEGLYVDQTYATAVILLLLVIIVNF
                     VSGLVAKRLGSESNSE"
     gene            2837..3586
                     /gene="pstB"
                     /locus_tag="VBC74_001359"
     CDS             2837..3586
                     /gene="pstB"
                     /locus_tag="VBC74_001359"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEO6942303.1"
                     /translation="MSKLVINNLDLYYGEFHALKDVNLDIEEKEITAFIGPSGCGKST
                     LLKSINRMNDLVKNCKITGDITLEGEDVYRQLDINQLRKKVGMVFQKPNPFPMSIYDN
                     VAFGPRTHGIHSKAELDDIVERSLKQAALWDEVKDRLHKSALGMSGGQQQRLCIARAL
                     AIEPDVLLMDEPTSALDPISTAKIEELVIQLKKNYTIVIVTHNMQQAVRISDKTAFFL
                     MGEVVEYNKTSQLFSLPQDERTENYITGRFG"
     gene            3583..4239
                     /gene="phoU"
                     /locus_tag="VBC74_001360"
     CDS             3583..4239
                     /gene="phoU"
                     /locus_tag="VBC74_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEO6942304.1"
                     /translation="MIRSRFASQLNDLNKEIIFMGALCEDIIGKSLGALTNSNDVYLD
                     DISETYHKIEQMERDIEERCLKLLLRQQPVAKDLRRISSALKMVYDMKRIGAQAYEIA
                     EIVSLGHIIQGAGSERDQLNSMSNNVISMLTKSIDAFIYDNEEQAHQVIEQDRTVNQE
                     FDTIKKQLVLYFSVQDVDGEYPIDVLMIAKYLERIGDHTVNIAKWVLFSITGNLDGEA
                     "
     gene            4239..4916
                     /locus_tag="VBC74_001361"
     CDS             4239..4916
                     /locus_tag="VBC74_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6942305.1"
                     /translation="MIYCVEDDADIREMMLYTLQMAGFTAQGFSSSELFWEAVQEKVP
                     DLILLDIMLPGDDGLTILERLRRKHQTEMIPVIMTTAKGSEYDKVKGLDLGADDYLVK
                     PFGMMEMISRIKAVLRRSRQVDSKAHIIIGNLEIDPTNYWVKRGTEKIHLTLKEFELL
                     VLFFRNPNRVFTRQELLDKVWGEQFLGETRTVDVHIGTLRTKLGEDGYLIATVRGVGY
                     RLEERHD"
     gene            4909..6564
                     /locus_tag="VBC74_001362"
     CDS             4909..6564
                     /locus_tag="VBC74_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEO6942306.1"
                     /translation="MTKKIFRTTLSASLGIVLVTILMIMGFLYNYFNRIQREQLRTQT
                     ALASQGISFEGKDYFENLKTSNVRITWVDNKGQVLYDTQSDAKHMKNHANRQEIKEAI
                     KSGYGESTRWSATLTEKSIYAAQRLNNGTIVRLSVAQQTIFYLLLGMMSPLAIIILLA
                     IILSVLIARYIAKKVSEPLNNIDLDHPLSNDSYEEITPLLRRLDSHQAKIQHQKLLLQ
                     KRQKEFDTIISKIKEGMILLDDQARIVSINAEALKLFQINDDWHGRFMMEVSRDLTLK
                     DLIDQGLKGKKKEANIDIENNHYRVLVRPTTDNNRVTGLVVLLFDVTDQLQMEQLQRE
                     FTANVSHELKTPLHVISGYSELLANQMVPNEEVPQFAAKIHKESERLVKLVEDIINLS
                     HLDEQEKLPQETVNLYDLTQKVLEGLQAKADKKHIQINFNGEEAILRGNPVLLNSLVY
                     NLCDNAITYNHEKGQVNVTLKNSPDTITLEVSDTGLGIAEKDKKRIFERFYRVDKSRS
                     KIVGGTGLGLSIVKSALDFHNGSIKVDSHLGQGTTMTVLLHKQ"
     gene            6719..8902
                     /locus_tag="VBC74_001363"
     CDS             6719..8902
                     /locus_tag="VBC74_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEO6942307.1"
                     /translation="MKNNVKQLFSFEFWQKFGKALMVVIAVMPAAGLMVSIGNSIRLL
                     DPSNVLLGRIANVIAQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLSFILINLI
                     TGNFFGVKTDMLANSKATVQTVFGATIRVSDYFVNVLGQPALNMGVFVGIISGFVGAT
                     AFNKYYNYRKLPDALTFFNGKRFVPFVVIYRSVIVALILSVFWPVVQSGINGFGKWIA
                     SSQDSAPILAPFVYGTLERLLLPFGLHHMLTIPMNYTQLGGTYTVLTGATKGAQVLGQ
                     DPLWLAWVGDLINLKGSNSSQYHHLLTSVTPARFKVGQMIGASGILMGLSYAMYRNVD
                     KDKKLKYKSMFISAAAATFLTGVTEPIEYMFMFAAMPLYLVYAVVQGCAFAMADIVNL
                     RVHSFGNIEFLTRVPMGIKAGLGGDIFNFVWVTLLFAVLMYFIANFMIKKFNLATAGR
                     NGNYDNEEVDNAPSTASGSADANSQVVQVINLLGGRDNIEDVDACMTRLRVTVKDGNS
                     VGSEAAWKKAGAMGLVLKGNGVQAIYGPKADVLKSDIQDLLDSGTVIPIVDLETGQPV
                     AATQVTTYKGITEEIVSVANGQVEALDVVKDPVFSQKMMGDGFAVEPTDGNIYVPVSG
                     TVTSVFPTKHAFGLLTESGLEVLVHIGLDTVALDGQPFEVKISSGQKVVAGDLAVVAD
                     LEAIKAAGKETSVIIVFTNVSDIKTVKLEKSGPQIAKTVVAKVEL"
     gene            8941..9771
                     /locus_tag="VBC74_001364"
     CDS             8941..9771
                     /locus_tag="VBC74_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEO6942308.1"
                     /translation="MKGYSMAKCLTLNTHSWMEVNALKKLFDLAEHIFREKYDIICLQ
                     EVNQSISSPLATSSPNYHPIEGTPALHQDNFALQLVHYLNLQGLHYHWTWAYNHIGYS
                     KYHEGVAILSLKPLKPDDILVSAVDDETDYHTRRALVAETTLNDKVVTVVSLHLSWFE
                     KGFAEEWKRLETTLLEVETPLLLMGDFNNPTGNQGYELVLNSPLALKDSHQIANHVFG
                     DHTIMADIDGWEGNKKALKVDHIFTSEDLSISSSQVVFEGGEAPVVSDHYGLEITLNL
                     "
BASE COUNT         3190 a         1582 c         2054 g         3219 t
ORIGIN      
        1 cattcgtaag ttaactctat aatgggatag ttatacttca attatgtggt ataacaactg
       61 tttcaagccg acttattggc tgaattacca taatagaaaa aaggagtttg gataattttc
      121 caactctttt taattttaca ttgattttac aaaaatcctc aaagagattt gatattcctt
      181 aactataata aaatcaaatc aaaaaatatt taggagttca tcatgaaaaa acacaaaatg
      241 ctatctcttt tggcggtatc tggtcttatg ggaattggca tcctagcagg atgctcaaat
      301 gattcatcat cttcatctaa agggacaatt aacattgttt ctagagagga agggtctgga
      361 actcgtggag ctttcataga gttatttggg atcgagtcaa aaaataaaaa gggcgaaaaa
      421 gtagaccata cttctgatgc agcaactgtt accaattcaa cttcagttat gctaaccact
      481 gtctctaaag acccgtcagc tattggttat tcttcactag gttcattaaa tagtagcgtt
      541 aaagtgctta aaattgatgg caaaaatgcg actgtaaaag atattaaatc aggaagctat
      601 aagatttctc gtcctttcaa tattgtaaca aaagaaggta aggaaaaaga agcaacaaaa
      661 gattttattg attatatctt aagcaaagat gggcaagcag tcgttgagaa aaatggctat
      721 attccacttg acaatgctaa agcatatcaa gcaaaagtgt catctggtaa agttgttata
      781 gcaggttcaa gttcagtaac gccggttatg gaaaagatta aagaagccta tcataaagta
      841 aatacaaaag tagatgttga aattcaacaa agtgattctt caactgggat tacaagcgca
      901 attgatggat cagcagatat aggtatggca tcacgtgaat tggataagac agagtcatcc
      961 aaaggggtca aagctactgt tattgcaaca gatggtattg cagtagttgt taataagaaa
     1021 aataaagtta atgatctatc aacaaaacaa gtaaaagaca tttttactgg aaagacaacg
     1081 tcttggtctg acttatcaaa gtaaggtaag agtaaacaat gaaacaaaca aaagaaggtc
     1141 ttatgcaagc tgtgtttttc atcacggctt gcgtcagtgt tatatcagct attttaattt
     1201 gcctctttct cttctctagt ggtcttccag ctattacaaa aatcggctgg ggaaatttta
     1261 tatttggtaa agtctggcat cctagtaata atatttttgg catctttcca atgattgtag
     1321 gttccttata tgttacagcg ggagctttac ttttaggagg cccgataggt atccttactg
     1381 ctgtttttat ggcttatttc tgcccagaaa atatttatag gcctttaaag tcagctatca
     1441 atttaatggc gggaattcct tcagttgttt atggtttctt tggactcgtt gtcattgtcc
     1501 ctatgataag gcaatatatt ggtggctttg gtatgggagt cttagcagct tcaattttgc
     1561 taggaattat gattttacca acaattgtta gcatttcaga atcttcttta agagctgttc
     1621 cagagagcta ttatgaaggc ggtattgccc ttggtgcctc tcatgaacga agtgtcttct
     1681 ttgctgtttt accagctgct aaacgtggta ttttagcatc ggttgttttg ggaattggac
     1741 gtgctattgg tgaaacaatg gctgtcatca tggtagcggg taatcaagct gtcttgcctc
     1801 aatcgctgac atcaggcgtt agaactctaa ccactaatat tgtcatggaa atgggatatt
     1861 cttctggttt gcatcgtcaa gccttaattg gtacagctgt ggtactcttt atctttatat
     1921 taatgattaa tatcagtttt tcagcattac agaaagagga ataagatgga aaatattaac
     1981 caaagaacgt caaaacaata tttagcatct ttaaaaaaag atcccctttc cttctttctt
     2041 tttgctattg tctatttggg agcaattcta agttttgcta caattgcatt tgttgttatt
     2101 tatattttag ttaaggggct tcctcatgtg aatactggct tgtttgcatg gacttataat
     2161 actcagaacg tctcactatt accagctttc attaacacta tctttatcat cgccttaact
     2221 ttattatttg cagttcccct tggtattggt ggatcaattt atctaacgga atatgctaga
     2281 cgtgataatc cttatctcaa aattatccgt gttgcaaccg agacattggc agggatacct
     2341 tcaattattt atggtttatt tggtgcatta ttttttgtaa aatacacaca cttaggctta
     2401 tctttgatat cgggttctct gactttgagc atcatgattt tacctttgat tatgaggaca
     2461 actgaggaag ctttactatc tgttccggat tcttatcgag aaggagcctt tgcgcttggt
     2521 gctggtaaat tgagaaccat atttaaaatc gtattaccta gtgctatgtc tggtattttt
     2581 gctggtatta ttttagcgat aggacgtatc attggtgagt cagcagcact tattttcaca
     2641 gctggaacag ttgcaaaagt agtacattcg gtatttagtt catctcgtac tttagcagta
     2701 catatgtatg ctatttcggg tgagggctta tatgttgacc aaacttatgc gactgcggtc
     2761 atcttattgc ttttagttat tattgtcaat tttgtatctg gactagtagc caaacgatta
     2821 ggaagtgagt cgaacagtga gtaaattagt aattaataat ttagatttgt attatggaga
     2881 atttcacgct ctgaaggatg ttaatttaga cattgaagaa aaagaaatca cagcctttat
     2941 tggaccttct gggtgcggaa agtctactct cttgaaaagt attaaccgta tgaatgattt
     3001 ggtcaaaaat tgcaaaatca caggggatat tactttagaa ggcgaggatg tctatagaca
     3061 attggatatt aaccaattgc gtaaaaaggt tggaatggtg tttcaaaaac ctaatccgtt
     3121 tccaatgagc atttatgata atgttgcttt tggtccaaga acgcacggta ttcattccaa
     3181 agcagagtta gatgatatcg ttgaacgttc ccttaaacag gcggctctat gggatgaggt
     3241 taaagatcgt cttcacaaat ctgctctggg tatgtctggc ggacagcagc aacgtttgtg
     3301 tattgcgcgt gctcttgcta ttgagcctga tgttttactt atggatgaac ccacaagtgc
     3361 cttggatcct atttcaacag caaaaattga agaattggtt atccaactga agaagaacta
     3421 cactattgtt atagtgacac ataatatgca acaagctgtt cgtatttctg ataaaacagc
     3481 attctttctg atgggagaag tggttgaata taacaaaacg agccaactct tttcattgcc
     3541 acaagatgag cgtacagaaa actacattac agggagattc ggatgattcg ttcacgattt
     3601 gcaagtcaac ttaatgacct taataaagaa attattttta tgggggcatt gtgtgaggac
     3661 atcattggga agtccttagg tgctctaaca aatagtaatg atgtttacct tgacgatatt
     3721 tctgaaactt atcacaagat tgaacaaatg gaacgggata ttgaggagag atgtttaaaa
     3781 cttttacttc gtcagcaacc agttgccaag gatttacgcc gtatttcttc tgcattaaaa
     3841 atggtttatg atatgaaacg tattggtgct caagcttatg aaattgcgga aattgttagt
     3901 ttaggtcata ttatccaagg tgcaggttct gaaagggatc aacttaacag catgtctaat
     3961 aatgtcatta gtatgctgac taagagtata gatgctttta tttatgacaa tgaagaacaa
     4021 gcgcatcaag taattgaaca agataggaca gttaaccagg aatttgatac aataaagaaa
     4081 cagttagtgt tatatttttc ggtacaggat gttgatggtg aatatcccat agatgtttta
     4141 atgatcgcca agtacctaga gcgtattggt gatcatacag taaatattgc taaatgggtg
     4201 cttttctcaa taacaggtaa cctagatgga gaagcgtaat gatatattgt gttgaagatg
     4261 atgctgatat tcgcgaaatg atgctctata ctttgcaaat ggcaggtttt acagctcaag
     4321 gattctcgag ttcagagctt ttttgggaag ctgttcagga gaaagttcca gacttgattt
     4381 tacttgatat tatgttgccg ggagatgatg gtttgactat tttagagcgt ttaaggagaa
     4441 aacatcaaac ggaaatgata ccagtaatca tgacaactgc taaaggtagt gagtatgata
     4501 aagttaaagg gttagatctt ggagcagatg attatctcgt caaacctttt gggatgatgg
     4561 aaatgatttc acgaataaaa gcagtcttga gacgtagccg ccaagtagat tcaaaagctc
     4621 atattatcat tggaaattta gagattgacc cgactaatta ttgggtcaaa aggggaacgg
     4681 aaaaaatcca cttaacccta aaggaatttg agttattagt attattcttc cgtaatccca
     4741 atagagtctt tacaaggcaa gaacttctgg ataaggtttg gggagaacaa tttttaggag
     4801 aaactagaac tgtggatgtt cacattggga cactgcgaac aaaacttggt gaggatggct
     4861 atttgatcgc tacagtaagg ggagttggat accgtttgga ggaaagacat gactaaaaaa
     4921 attttccgta caactttatc cgctagtttg ggaattgttt tagttacaat tttgatgatt
     4981 atgggatttc tctataatta cttcaatcgc attcagcgag aacagttgag aacacaaacg
     5041 gcactggcgt cacaaggaat ttcctttgaa ggtaaagatt attttgaaaa tcttaaaact
     5101 tcaaatgtgc gtattacttg ggtagataat aagggacaag tgctttatga tacccaatca
     5161 gatgcaaaac atatgaaaaa tcacgccaat cgtcaagaaa ttaaagaagc tatcaagagt
     5221 ggttatggtg agagcacgag gtggtcagcg acacttactg agaagtctat ctatgctgcg
     5281 caacgtttaa ataatggaac gatagttcgt ttatcagttg cgcaacaaac tattttttat
     5341 cttctccttg gtatgatgag tcccttagca attatcatct tacttgctat tattttatct
     5401 gtattaattg cacgatatat agcaaaaaaa gtatcagagc ctttaaacaa tattgactta
     5461 gatcatcctt tatcaaatga tagttacgag gagataacac cattattaag gcgtcttgat
     5521 agccatcagg ctaagattca acatcaaaaa ctgttattac aaaagcgtca aaaagaattt
     5581 gataccatta tttctaaaat taaggaagga atgattcttt tagatgatca agcaagaatc
     5641 gttagtatca acgcagaagc tttaaagtta ttccaaatta atgatgattg gcatgggcgc
     5701 tttatgatgg aagttagccg agatttaact ctcaaagact taattgatca aggactaaaa
     5761 gggaaaaaga aagaggctaa tattgacatt gaaaataatc attaccgcgt cttggtaaga
     5821 ccaactacag ataacaaccg agtaacagga ttggttgttc tactgtttga tgtgactgac
     5881 caactccaaa tggaacaact tcaacgtgaa tttactgcca atgtctctca tgaattgaaa
     5941 accccgcttc atgttatttc tggttactca gaactgttag ctaatcaaat ggttccaaac
     6001 gaagaagtcc cacaatttgc agctaaaatt cataaagaat ctgaacgatt agttaaattg
     6061 gttgaggata ttatcaacct ttctcacctt gatgaacaag aaaaattacc gcaagagaca
     6121 gtaaatctgt atgacttaac acaaaaagtt ttagaaggtt tacaagctaa agcagataag
     6181 aagcacatac aaatcaattt taatggagaa gaagctattt tgcgagggaa tccagtttta
     6241 ttgaattcct tggtgtacaa cttgtgtgac aatgctatca cctataatca tgagaaaggt
     6301 caagtaaatg tgactttgaa aaattcacct gatacaatta ccttagaagt tagtgatacg
     6361 ggactaggca ttgcagagaa ggacaaaaaa cgtattttcg aacgtttcta tcgcgtcgat
     6421 aaaagtcgct caaaaatagt tggagggact ggtttaggtt tgtctattgt aaagagtgct
     6481 ttagattttc acaatggtag tataaaagtg gacagccatt tggggcaggg aacaacaatg
     6541 accgttttac ttcacaaaca ataaaaactc acaaataaaa gtctagatga tattatctag
     6601 acttttttag taattcagaa aaaatatata agtaagggtt tacaaaaaac aaataagtgc
     6661 tataataaaa ccataacgca aacgtttgca ctattaagtg cataaaagga gattgaagat
     6721 gaaaaataat gtcaaacagc tctttagttt tgaattttgg cagaagtttg ggaaggcttt
     6781 gatggttgtt attgctgtta tgccagcagc aggattgatg gttagtattg gtaactccat
     6841 taggctattg gatccatcaa atgttttatt agggcgcata gctaatgtta ttgctcaaat
     6901 tggttgggga gttattggta acttacacat cttatttgca ttggctattg gtggtagctg
     6961 ggctaaagaa cgtgccggtg gtgcttttgc ggctggctta tcctttattc ttattaacct
     7021 tattacaggg aacttttttg gtgtgaaaac agatatgtta gcaaacagta aagcaactgt
     7081 tcaaacggta tttggtgcga ccatcagagt ttcagattac tttgttaacg ttcttggaca
     7141 acctgctctt aatatgggtg tatttgtcgg tattatttca ggtttcgttg gtgcaactgc
     7201 ctttaataaa tattataact atcgtaaatt accagatgct ttaacttttt tcaatggaaa
     7261 acgttttgtt ccatttgttg ttatttatcg ttcagtcatt gttgcattaa ttttatcagt
     7321 attttggccg gttgttcaat caggtatcaa tggctttggt aagtggattg caagttcaca
     7381 agacagtgct ccaatattag caccatttgt ctacggtacg ttagagcgtt tattgctccc
     7441 atttggttta caccatatgt taactattcc aatgaactac acgcaactag gtggcacata
     7501 cactgtcctt acaggtgcaa ctaaaggagc acaggtgttg ggacaagatc cactgtggtt
     7561 agcatgggtt ggtgatttaa tcaacttaaa aggctcaaat tcaagtcaat accatcactt
     7621 attgacaagt gtcactccag cacgttttaa agtggggcaa atgattggtg cttcaggtat
     7681 tttgatggga ttatcttatg ctatgtatcg taacgttgat aaagataaaa aattgaaata
     7741 caagtcaatg tttatttcag cagcagcggc tactttcttg acaggtgtta ctgagccaat
     7801 tgagtacatg ttcatgtttg cagcaatgcc actctacctt gtatatgctg tcgttcaagg
     7861 atgtgcgttt gctatggcag atattgttaa cctccgcgtt cattcatttg gtaatataga
     7921 atttttaaca cgtgttccca tgggaataaa agcgggcctt ggtggtgata ttttcaactt
     7981 tgtttgggtg acacttcttt ttgcagtatt gatgtacttt attgcaaact ttatgattaa
     8041 aaaattcaat ttagcaacag caggacgtaa tggtaattat gataacgaag aggttgataa
     8101 tgctccttca acagcttctg gttctgctga tgcaaattct caagttgtac aagttattaa
     8161 tttacttggt ggtcgtgata acattgaaga tgtagatgca tgtatgactc gtcttcgtgt
     8221 cacagttaaa gatgggaata gcgtgggttc tgaagcagct tggaaaaaag ctggtgcaat
     8281 gggattggtg ttaaaaggta atggcgttca agctatttac ggacctaaag cagatgtcct
     8341 taaatcagac attcaagatt tgctagattc aggtacagtg attccaatag ttgatttaga
     8401 aacaggccag cctgtagcag caacccaagt tacaacttat aaaggaataa cagaagaaat
     8461 tgtttcggtg gctaatggtc aagtagaagc attggatgtt gttaaagacc ctgtcttttc
     8521 tcaaaaaatg atgggtgatg gctttgctgt ggaaccaact gatggcaata tctatgttcc
     8581 agtttcaggt acagttacta gtgtctttcc aacaaaacat gcttttggtt tattaacaga
     8641 aagtggccta gaagttttag tacatatcgg tctagataca gtagctcttg atggtcaacc
     8701 atttgaggtt aaaatttcta gtggtcaaaa agtggtagca ggcgatcttg ctgtagtagc
     8761 agatttggaa gctatcaaag cagctggtaa agagacaagt gtgattatcg tctttacaaa
     8821 tgtttcagat ataaaaactg ttaaacttga aaaatcaggt ccacaaatag ctaaaacagt
     8881 tgtggcaaaa gtcgaactgt aacaaacgat aggagctggg caagtctcag cttctttctg
     8941 atgaaaggat actctatggc aaaatgctta acgttaaata ctcattcatg gatggaagta
     9001 aatgctctaa agaaattatt tgatttagca gagcatatct tccgtgaaaa atatgatatc
     9061 atctgcctac aagaggtgaa ccagagcata tcttcccctc ttgctacgag tagtccaaat
     9121 tatcatccga ttgaaggtac accagctctt catcaagata actttgcctt gcaattagtg
     9181 cattacctaa atcttcaagg tctacactat cattggacat gggcttataa tcatattggt
     9241 tatagtaagt accatgaagg ggtagcgata ctttctctga aaccattaaa gccagatgat
     9301 attttagttt cagctgtaga cgatgagact gattatcata ctcgtcgtgc tctggtagct
     9361 gagaccactc tgaatgacaa agtcgttaca gtagttagcc tccatttatc atggtttgag
     9421 aaaggatttg cggaggagtg gaaacgacta gaaactaccc tacttgaggt agaaactcca
     9481 cttttactca tgggtgactt taataatcct actggaaatc agggttatga acttgttctt
     9541 aatagtccat tagcattaaa ggatagccac caaatagcca atcatgtatt tggagaccat
     9601 actattatgg ctgatattga tggttgggaa gggaataaaa aggctcttaa agttgaccat
     9661 atttttacta gcgaagacct ttcaatcagt tcaagtcaag tagtttttga aggtggtgaa
     9721 gcacctgtcg ttagtgatca ttacggcttg gaaattacgt taaatcttta gattaatcta
     9781 tttaatatat taaaagcatc ctaaatttgt ctataaaaca aaacttaggg tgctttttga
     9841 aaaatatgtc acattattta acttcttacg aagtttggaa tgcatgagaa aatatgataa
     9901 aatgataata actattatag gtgattttaa taaataattt aatcaattgg tttatctatt
     9961 agaacaatta atacgaggag acgagagtga atatttttat tttagaggat aattttattc
    10021 aacaggcacg tttagagaga ataat
//
LOCUS       DATROD010000061         9975 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.061, whole genome shotgun sequence.
ACCESSION   DATROD010000061 DATROD010000000
VERSION     DATROD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9975
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.061"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..70)
                     /locus_tag="VBC74_001365"
     CDS             complement(<1..70)
                     /locus_tag="VBC74_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEO6942309.1"
                     /translation="MKVALIPEKCIACGLCQTYSNIF"
     gene            116..640
                     /locus_tag="VBC74_001366"
     CDS             116..640
                     /locus_tag="VBC74_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEO6942310.1"
                     /translation="MAKKPWEKKVVENNSHRKDKITRTSRGVVSSTPWITAFLSAFFV
                     IVVAILFIVFYTSNRGEDRAKETSGFYGASSQKVNSSKTKKASTSKKTTSSSKTKTSD
                     KSTTSSSTVASSSETSTSSSVNGGTIVVQPGEGAASIAARAGISVARLQELNPGHMTS
                     GYWYANPGDPVVVN"
     gene            651..1334
                     /locus_tag="VBC74_001367"
     CDS             651..1334
                     /locus_tag="VBC74_001367"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEO6942311.1"
                     /translation="MNSINIAIDGPASSGKSTVAKIIAKNLNYTYLDTGAMYRCATYL
                     ALQHGYEAQDVSKILGLLAERPISFGKAEDGSQTVFLGTEEVTLAIRQNDVTNNVSWV
                     SAIPEIREELVNQQRRIAKDGAIIMDGRDIGTVVLPDAELKIFLVASVDERAERRFKE
                     NQEKGIESDFETLKSEIAARDYKDSHREVSPLKAAEDAIEFDTTGVSIEGVVTFIQEK
                     AEKIIDMKN"
     misc_feature    1355..1480
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1495..2025
                     /gene="infC"
                     /locus_tag="VBC74_001368"
     CDS             1495..2025
                     /gene="infC"
                     /locus_tag="VBC74_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011226065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEO6942312.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQAIADD
                     ANVDLVLIQPQATPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPIPDKK"
     gene            2065..2265
                     /gene="rpmI"
                     /locus_tag="VBC74_001369"
     CDS             2065..2265
                     /gene="rpmI"
                     /locus_tag="VBC74_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEO6942313.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFHAFTSHRFHGKTKKQRRHL
                     RKATMVSSGDFKRIKAMLTRLK"
     gene            2323..2682
                     /gene="rplT"
                     /locus_tag="VBC74_001370"
     CDS             2323..2682
                     /gene="rplT"
                     /locus_tag="VBC74_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEO6942314.1"
                     /translation="MARVKGGVVSRKRRKRVLKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AAAFTALADAAKAKLGK"
     gene            complement(2925..5069)
                     /locus_tag="VBC74_001371"
     CDS             complement(2925..5069)
                     /locus_tag="VBC74_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEO6942315.1"
                     /translation="MKKIKDFASRAINTRLGFILLLVVIYWLKTIWAYHTDFNLGLEN
                     SYQLFLTIINPIPLGLLIIGLALYVKRTKAFYITAFITYAIVNILLIANAIYYREFSD
                     FITVSAVLASSKTSAGLGDSALNLLRIWDLVYVFDFIILIFLFATKKIHLDNRPFNKR
                     ASFSITALSGLLFSINLFLAEIDRPELLSRGFSNTYIVKALGLPSFSIYSGNQTYQAQ
                     KERNGATAQELATAKKYVAEHYAKPNPEYYGIGKGRNVIMIHLESFQQFLIDYKLNVD
                     GKEHVVTPFINSLYHSKETISFSNFFHQVKAGKTSDAETLMENSLFGLSSGSFMVNYG
                     GENTQFAAPHILAQNGGYSSAVFHGNVGTFWNRNNAYKQWGYDYFFDSSYFSKQTKDN
                     SFQYGLNDKYMFADSIKYLEHMQQPFYTKFITVSNHYPYTSLKGESDEEGFPLAKTND
                     ETINGYFATANYLDTALKSFFEYLKAAGVYDNSIIVMYGDHYGISNTRNPSLAELLGK
                     DPETWSEYDNAMLQRVPYMIHIPGYSKGFISNTYGGEVDNLPTLLHILGIDTSKYTQL
                     GQDLLSKDNKQMVAMRTTGQYITPKYTNYSGHLYYTDSGQEITNPDETTKAEIKTIRD
                     ATNKQLSTSDSIQTGDLLRFDENNGLKTVEVEKFNYTHSLKALKAKERKLKDRSTSIY
                     SKHNNKSTVDLFHAPSYLELQDPNKTHKTSKK"
     gene            5204..6361
                     /locus_tag="VBC74_001372"
     CDS             5204..6361
                     /locus_tag="VBC74_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEO6942316.1"
                     /translation="MNKLKVNSVVERKIKSGAQLLEKKDFDTSLVNQLVQLFSQSNQF
                     LGMAYLSPQNKGIGWLLSRQIFDFNHVYFVSLFEKSREKRQKFEKSSQTTAYRLFNQD
                     GDNFGGLTIDFYSDYALFSWYNEFVYTNRQMIVAAFKQVYPNIKGAYEKIRFKGLDFE
                     SAHLYGQEAPESFLILENNIKYSVFLNDGLMTGIFLDQHDVRKALATNLSEGKKVLNM
                     FSYTAAFSVAAAVGGALETTSVDLAKRSRELSKAHFDANQIVTDNHRFIVMDVFEYYK
                     YAKRKHLSYDVIVIDPPSFARNKKQTFSVTKDYYKLIEQALDILAPGGTIIASTNAAN
                     LTVSQFKKQLEKGFGKASHNYISLQQLPEDFTVNDKDQQSNYLKVFTIKVK"
     gene            6361..7038
                     /locus_tag="VBC74_001373"
     CDS             6361..7038
                     /locus_tag="VBC74_001373"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEO6942317.1"
                     /translation="MKIVVPVMPRSLEEAQEIDLSKFDSVDIIEWRADALPKDDIINV
                     APAIFEKFAGHEIIFTLRTTREGGNIVLSDAEYVELIQKINSIYNPDYIDFEYFSHKE
                     VFQEMLEFPNLVLSYHNFQETPENIMEIFSELTALAPRVVKIAVMPKNEQDVLDVMNY
                     TRGFKTINPDQVYATVSMSKIGRISRFAGDVTGSSWTFAYLDSSIAPGQITISEMKRV
                     KALLDAD"
     gene            7132..8199
                     /locus_tag="VBC74_001374"
     CDS             7132..8199
                     /locus_tag="VBC74_001374"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEO6942318.1"
                     /translation="MQVEVDLPNHPYHIKIEEGCFSEAGDWVSHLWQKQMITIITDSN
                     VEILYGESLVNQLKKQGFTVHVFSFAAGEASKTLEVANRIYGFLAKHHMTRSDGIIAL
                     GGGVVGDLAAFVASTYMRGIHFLQIPTSLTAQVDSSIGGKTGVNTSFAKNMVGTFAQP
                     DGVLIDPVTLKTLGNRELVEGMGEVIKYGLIDDIKLWHILEEMDGSIDSILDNALAII
                     YHSCQVKRKHVLADQYDKGLRMHLNFGHTIGHAIEVHAGYGEIMHGEAVAIGMIQLSR
                     VAERKNLMPRGISQDIYNMCLKFGLPVHYAEWDKDVLFNILSHDKKASGQFIKIVILP
                     QLGSATVHQIPLEEMRDYLEK"
     gene            8200..9366
                     /gene="aroC"
                     /locus_tag="VBC74_001375"
     CDS             8200..9366
                     /gene="aroC"
                     /locus_tag="VBC74_001375"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEO6942319.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLKLSAKDINEDLKRRQGGYG
                     RGNRMKIETDQVIISSGVRHGKTLGSPITLTVTNKDHSKWLDIMSVEDIEERLKQKRR
                     IKHPRPGHADLVGGIKYRFDDLRNALERSSARETTMRVAIGAIAKRILKEIGIEIANH
                     IVVFGGKEITVPDKLTVQQIKVLSSQSQVAIVNPSFEQEIKDYIDSVKKAGDTIGGVI
                     ETIVGGVPVGLGSYVHWDRKLDAKIAQAVVSINAFKGVEFGLGFKSGFLKGSQVMDSI
                     SWTKDQGYIRQSNNLGGFEGGMTNGEPIVVRGVMKPIPTLYKPLMSVDIDTHEPYRAT
                     VERSDPTALPAAGVVMEAVVATVLVTEVLEKFSSDNMYELKEAVKLYRNYVNHF"
     gene            9450..9785
                     /locus_tag="VBC74_001376"
     CDS             9450..9785
                     /locus_tag="VBC74_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEO6942320.1"
                     /translation="MANVYDLANELERAVRALPEYQAVLTAKAAIENDADAQVLWQDF
                     LATQSKVQEMMQSGQMPSQEEQDEMSKLGEKIESNDLLKVYFDQQQRLSVYMSDIEKI
                     VFAPMQDLM"
BASE COUNT         3293 a         1570 c         1980 g         3132 t
ORIGIN      
        1 caaaaatatt tgaatatgtt tggcaaagcc cgcaggcaat acatttttct ggaattagtg
       61 caactttcat agtattattg taatacttat tgatacgatt aacaaggggg aagtcatggc
      121 gaaaaaacca tgggaaaaaa aagttgtgga aaacaatagt catagaaaag acaaaattac
      181 aagaacgagt agaggtgtgg tgtcaagtac tccttggatt accgctttct tgagtgcatt
      241 ctttgttatt gtcgttgcta ttttatttat agtattttat acttcaaata gaggagaaga
      301 cagagcaaaa gaaacttctg gtttttatgg tgcttcaagt caaaaagtta atagttctaa
      361 aacgaaaaaa gcatctacaa gtaagaaaac aacgtcatca tcaaaaacta aaactagtga
      421 taaatcaaca acatcttcta gtacagtagc aagttcgagt gagacatcga catcatcttc
      481 agtaaacggt ggaacaatcg tagttcaacc aggtgaggga gcagcctcca tagctgctcg
      541 tgcaggtatc tcagttgctc gtttacaaga acttaatcct ggtcatatga cctcagggta
      601 ttggtatgct aatccaggcg atcctgtggt tgtaaattaa ggggaggatt atgaactcaa
      661 ttaatattgc tattgatgga ccagcatcaa gtgggaaaag tactgtcgct aagattattg
      721 ctaaaaattt gaattacact taccttgata caggtgctat gtatcgttgt gctacttatc
      781 tggctttaca gcatggatat gaagcacaag atgtttcgaa aattttagga ctcctagctg
      841 aaagaccgat ttcttttggt aaagcagaag atggcagcca gacagtcttt cttggcacag
      901 aagaagttac tttagctatc cgtcaaaacg atgttactaa caatgtctcg tgggtttctg
      961 ctatcccaga gattcgagaa gagttagtaa accagcagcg tcgtatagcc aaagatggtg
     1021 ctattataat ggatggtcgt gacataggaa cagttgtttt gccagatgca gaattgaaga
     1081 tttttttagt ggcttctgtt gatgaaagag ctgagcgtcg ctttaaagaa aatcaagaaa
     1141 aaggcattga gagtgatttt gaaacactga aatcagaaat tgctgctcgt gattataaag
     1201 atagccatcg agaagtttca ccattaaagg cagcagaaga tgctattgaa tttgatacta
     1261 caggagtatc aattgaaggt gttgtaactt ttattcaaga aaaagcagaa aaaataattg
     1321 acatgaagaa ttagtcatga tatactagtt gcaatgagaa aagcagaagt gagaacttct
     1381 cgccttgcga cttaggttgt ctggcccaac atgtcaagat tccatctagg aagattatat
     1441 gtgcgggctt gatttatcaa gtccgctttc gtttttctct aaaaaaataa agaggtgaag
     1501 atcatagcta aaaaagatct attcattaac gatgaaattc gcgttcgtga agttcgtctg
     1561 gttggtctcg agggtgaaca attaggtatt aaaccattgt ctgaagctca agcaattgca
     1621 gatgatgcta atgttgattt ggttttaatt cagccacaag ctacaccacc tgttgcgaaa
     1681 attatggact atggtaagtt caaatttgag tatcaaaaga aacaaaaaga acaacgtaaa
     1741 aaacaaagcg ttgttactgt taaggaagtt cgtcttagcc cagttattga taaaggggat
     1801 tttgaaacaa aacttcgtaa cggtcgtaag ttccttgaaa aaggtaacaa agttaaagtt
     1861 tctatccgat tcaaaggacg tatgattact cataaagaaa ttggagcaaa ggtattggcg
     1921 gaatttgctg aagcgacaca agatatcgct attattgagc aaagagctaa gatggatggt
     1981 cgccaaatgt ttatgcagct tgcaccaatt ccagacaaga aataattgtc gactttatag
     2041 tattatttaa gaggagaatt taaaatgcca aaacaaaaaa cacaccgcgc atcagctaaa
     2101 cgctttaaac gcacaggttc aggtggattg aaacgcttcc acgcgtttac ttcacaccgt
     2161 ttccacggta aaactaaaaa acaacgtcgt catcttcgta aagctactat ggtttcatca
     2221 ggagatttca aacgtattaa agcaatgctt actcgcctta aataataagg tctctgtaaa
     2281 catttactac atattataaa ggattatttg gaggaaatat aaatggctcg tgttaaaggt
     2341 ggagttgttt cacgtaaacg tcgtaaacgc gtacttaaat tagctaaagg ttactatggt
     2401 gcaaaacata tcttgttccg tactgcaaaa gaacaagtaa tgaattctta ctactatgca
     2461 taccgtgacc gtcgtcagaa aaaacgtgac ttccgtaaat tatggatcac tcgtatcaat
     2521 gcggctgctc gtatgaatgg tttatcatat tcacaattga tgcacggttt gaaattagct
     2581 gaaatcgaag ttaaccgtaa aatgcttgct gatttagcag ttaacgatgc agcagctttc
     2641 acagctcttg cagatgcagc taaagctaaa cttggtaaat aagaaataaa aaagttgggt
     2701 ctgcctaact ttttttaatt actagaaaga aagtacctat attataattt aagtgaaaag
     2761 tttcgttcta tatatgtaga atgaaacttt ttttgtttta ttccgatacc tttattattg
     2821 ttataatttt cctttttgat ttttattgtt ttatagtatt attccgtata aaaagcacct
     2881 caatcgttgg attgaggtgt ggattaaact ttaaatggta aagattattt tttagatgtt
     2941 ttatgtgtct tattaggatc ttgcaattcc aagtaacttg gagcgtggaa gagatcaact
     3001 gttgatttgt tgttatgttt gctgtatatg cttgttgaac gatctttcaa tttacgttct
     3061 ttagctttga gtgctttaag agaatgtgtg taattgaatt tttcaacttc tacagttttc
     3121 aaaccattgt tttcatcaaa tcgtagtaaa tctccagttt gaatcgaatc acttgtagat
     3181 aattgtttat tagtagcatc tcgaatagtt tttatttcag ctttagttgt ttcatcagga
     3241 tttgtgattt cttgtcctga atcagtgtaa tagagatgtc cactatagtt agtgtattta
     3301 ggagtaatat actgaccagt tgttctcata gcgaccattt gtttattatc tttagataac
     3361 aagtcttgtc ctaactgagt atatttagac gtgtctatcc ctagaatatg aagtaaggtt
     3421 ggtaaattat ctacctcacc accgtatgta ttactgataa aaccttttga atagccagga
     3481 atgtggatca tatatggaac acgttgtaac atagcgttgt cgtattcaga ccatgtttca
     3541 gggtctttac ctaataattc agctaagcta ggattgcgtg tattagaaat accgtagtga
     3601 tccccataca ttacaataat agaattatca tacacaccag cagcttttag atattcaaaa
     3661 aatgatttaa gagctgtatc aaggtagttt gctgtggcga aataaccgtt aatcgtttca
     3721 tcgttagtct ttgcaagtgg gaatccctct tcgtcagact ctcccttcaa acttgtgtaa
     3781 gggtaatggt tacttactgt aataaattta gtataaaacg gttgttgcat gtgctctaga
     3841 tatttgatag agtcagcaaa catatattta tcattcaaac cgtattggaa tgaattatct
     3901 ttggtttgtt ttgagaagta gctagagtca aagaagtagt cataacccca ctgtttgtaa
     3961 gcattatttc tattccaaaa tgttccgacg tttccgtgga agaccgcact actgtaacct
     4021 ccattttgag ctaagatatg tggagcagca aattgtgtat tttcaccacc ataattaacc
     4081 atgaatgaac cactacttag accaaaaagt gagttttcca tgagtgtctc agcatcggat
     4141 gtcttaccag ctttgacttg atggaagaag ttagagaagg agatagtctc tttcgaatga
     4201 taaaggctgt taataaatgg agtaaccaca tgttctttac catcaacatt taatttatag
     4261 tcaatcaaga attgttgaaa actctccaaa tgaatcatga taacgtttcg cccttttccg
     4321 ataccataat attctggatt gggtttggca taatgctctg ctacatattt tttggcagtc
     4381 gctaattctt gtgcagtggc accatttctt tctttctgtg cttgataagt ttgattacca
     4441 ctataaatcg aaaatgatgg caagccaaga gctttaacaa tataggtgtt tgaaaaacca
     4501 cgacttagta gttctggacg atcaatttca gctaagaata agttaattga aaagagtaaa
     4561 ccagacaaag ctgtgataga aaaactagca cgtttattaa atggacgatt atctaagtgg
     4621 attttcttgg ttgcaaataa gaaaataagg ataatgaaat cgaaaacgta gacaagatcc
     4681 caaattctca atagattaag agcagagtct ccaaggccag ccgaagtttt actactagcc
     4741 aagacagcgc taactgtaat gaaatcggaa aactctctat agtagattgc gtttgcaatt
     4801 agaaggatat ttacgatagc ataggtgata aaagcggtaa tataaaaagc tttggttcgc
     4861 ttaacatata aggccaaacc tatgattaag agtcctaagg ggatggggtt tataatggtc
     4921 aaaaacaact gataggaatt ttctaaacct aaattaaaat cagtatggta agcccaaatt
     4981 gtctttaacc aatatataac tactaacaat aaaataaagc cgagtcgtgt attgatcgca
     5041 cggctagcaa aatctttaat ttttttcaca gtaggaactt cctttaattt ttttcctaaa
     5101 gataaaatct cagattaaat tataacataa aattttgtaa catacggtaa agaaaggtcg
     5161 aattcagtat gaaaaaagat aaaaatttgt tataatactg tttatgaata aacttaaagt
     5221 gaattctgtc gttgaaagaa aaatcaaatc aggtgctcag ttactggaaa aaaaagattt
     5281 tgataccagt ttagttaacc agttggttca acttttttca cagtcaaatc aattcttagg
     5341 gatggcctat ctttcaccac aaaataaagg gattggttgg ttactatcaa gacaaatttt
     5401 tgattttaac catgtttact ttgtatcgct attcgaaaaa tctagagaga agcgtcaaaa
     5461 atttgaaaaa tctagccaaa caacagccta ccgcttgttt aatcaggatg gagataactt
     5521 tggtggccta actatagatt tttatagtga ctatgctctt ttttcgtggt acaatgaatt
     5581 tgtttatact aatcgacaaa tgattgttgc agcctttaag caggtctatc ctaatattaa
     5641 aggggcatat gaaaaaattc gtttcaaagg tttagacttt gaaagtgctc atttgtacgg
     5701 tcaagaggct cctgaatcat ttttgatttt agaaaataat atcaaatata gtgtcttttt
     5761 gaatgatggg ttaatgacag gtattttcct tgaccaacat gatgtcagaa aagccttagc
     5821 aactaatcta tcagaaggta aaaaagttct aaatatgttt tcatacactg cggctttttc
     5881 tgtagctgca gctgttggag gagcattaga gacaacttct gttgatttag caaaacgctc
     5941 gcgtgaactt tcaaaagcac actttgatgc taatcagatt gtcacagata accatcgatt
     6001 tatcgttatg gatgtttttg aatattataa gtatgccaaa agaaaacatc tatcatatga
     6061 tgtgattgtt attgatccac caagttttgc tcgaaataaa aaacaaactt tttcggttac
     6121 taaagattac tataaattaa ttgaacaagc tttagatatt ttagcccctg gtggaactat
     6181 cattgcatca actaacgcag ctaatctaac cgtatcacaa tttaagaaac aattggaaaa
     6241 ggggtttggt aaagcttcac ataattacat tagcttacag cagttgcctg aagatttcac
     6301 tgtaaatgat aaggaccaac aaagtaatta tttaaaagta tttacaataa aggtaaaata
     6361 atgaaaatag tagtaccagt aatgcctcgc agtcttgaag aggctcaaga aatagattta
     6421 tcaaaatttg atagtgttga tattattgaa tggcgagctg atgccttacc aaaggatgac
     6481 attattaatg tagctccagc tatttttgag aaattcgcag gtcatgaaat tatttttact
     6541 cttcgtacaa cgcgtgaagg tggtaatatt gtcttatctg atgctgagta tgttgagtta
     6601 atccagaaaa ttaattctat ctacaatcca gattatattg attttgagta tttttcacat
     6661 aaagaagttt ttcaagaaat gctagaattt ccaaatttag tcctgtctta tcacaatttt
     6721 caagagacac cggagaatat tatggagata ttttcagaat taacagccct agcaccacga
     6781 gttgtgaaaa tcgcagtaat gccaaagaat gaacaagatg tcttagacgt tatgaattac
     6841 actcgcggtt tcaagactat taatcctgat caagtttatg cgacggtatc tatgagtaaa
     6901 attggacgta tttctcgttt tgctggtgat gtaactggat ctagttggac atttgcatat
     6961 ttagattcat ctatcgcacc cggacaaatt actatttcag agatgaagcg tgtcaaagca
     7021 ttgcttgacg ctgactgata cgagataaca aaatgttaaa tatactgatg agatccctag
     7081 caattgctta gggtttttgt cttattaaaa caaacatgag aggacattag aatgcaggtt
     7141 gaagttgact taccgaatca cccttatcat attaaaattg aagaaggatg tttttctgag
     7201 gctggtgact gggtatcaca tttatggcag aaacaaatga taactattat tacagatagt
     7261 aatgtagaaa tattatacgg ggagtcgctt gtcaaccagt taaaaaaaca aggttttaca
     7321 gtacatgttt ttagttttgc tgcgggtgaa gctagtaaaa ctttggaagt tgctaatcgt
     7381 atttatggtt ttttagcaaa gcatcatatg accagaagtg atgggatcat tgcacttggt
     7441 ggaggcgtcg tcggagattt agcagcgttt gttgcttcta cttatatgcg gggaatccat
     7501 tttcttcaaa ttccaactag tttaacagca caggttgatt cttccattgg cggaaaaaca
     7561 ggagtcaaca cttcattcgc taaaaatatg gtaggtactt ttgcacaacc tgatggtgta
     7621 ctgatagatc ctgttactct aaaaacattg ggcaatcgtg aattagttga aggtatggga
     7681 gaagtcatca aatatggctt gattgatgat ataaaactct ggcatatctt agaagagatg
     7741 gatgggtcta ttgatagtat ccttgataat gcattagcta ttatttatca ctcttgtcaa
     7801 gttaaacgaa agcatgtttt agctgatcaa tatgataaag ggttaaggat gcatttaaat
     7861 tttggccaca ctatcggtca tgctatcgaa gtacatgctg gctatggaga aataatgcat
     7921 ggagaagctg ttgcaattgg aatgatccag ctttctcgtg tggctgagcg taaaaatctt
     7981 atgccaagag gtatcagtca agatatttat aatatgtgtt taaaatttgg ccttccggtc
     8041 cactatgcag aatgggataa ggacgtctta tttaacatat tgagtcatga taaaaaagca
     8101 agtggacaat ttattaaaat tgttatactg cctcaattag gtagtgcaac agtccaccaa
     8161 atacctttag aagaaatgag agattatttg gagaaataga tgagatattt aactgctggt
     8221 gagtcacatg gacctagttt aacagctatt atcgaaggga ttcctgcggg actgaaatta
     8281 tctgctaaag atattaatga agatttaaaa cgccgccaag gtggttatgg tcgtgggaat
     8341 cgaatgaaaa ttgaaactga tcaagtgatc attagttctg gtgtgcgcca tggaaaaacc
     8401 ttgggttcac ctattacact gacagtaact aataaggatc atagcaaatg gctggacatc
     8461 atgtcagtcg aagacattga agagcgtcta aagcaaaaac gtcgtattaa gcatcctaga
     8521 cctgggcacg cagatttagt aggtggaatc aaataccgat ttgatgattt gagaaatgct
     8581 ttagagcgtt cttcggctcg tgagaccaca atgcgagtag ctattggtgc gattgctaaa
     8641 cgtatcttga aagaaattgg tatcgaaatt gctaaccata ttgtagtttt cggtggcaag
     8701 gagattactg tccctgataa gctaacagtt caacaaatca aagtgttatc tagtcaatcg
     8761 caagtagcta tcgtcaatcc atcatttgaa caagaaatta aggattatat tgacagtgtg
     8821 aaaaaggctg gagatactat cggaggagtg attgaaacta tcgttggagg agttcccgtt
     8881 ggtttaggct cttatgtaca ctgggataga aagttagatg ctaagatagc gcaagcagtt
     8941 gtatctatca atgcttttaa aggtgttgaa tttggtctag gttttaagtc agggttttta
     9001 aaaggaagtc aagtaatgga ctccatatca tggacaaagg accaaggtta catacgtcag
     9061 tcaaataatt taggcggttt tgaaggcggt atgactaatg gagagcctat tgtcgttagg
     9121 ggagtaatga agccaattcc tacgctttac aagccactaa tgtcagttga tattgatact
     9181 cacgagcctt atagagctac agtggaacgt tctgatccca cggcacttcc ggctgcaggt
     9241 gtcgttatgg aagctgtagt agccaccgtc ctggtgacag aagtgctaga aaaattttct
     9301 tcagacaaca tgtatgaact aaaagaagct gtaaaactat atcgcaatta tgttaatcat
     9361 ttttaagtga aaactagtag cattcaattg caaaaaaagg tataaaagtc tacaatagtg
     9421 gtaagttcaa gtagaaaaag gagtaactta tggctaatgt atatgattta gcaaatgaat
     9481 tagaacgtgc tgttcgtgct ttaccagaat accaagcagt tttaactgca aaagcagcta
     9541 ttgaaaatga tgcggatgca caagtgcttt ggcaagactt tttggctacc caatcaaaag
     9601 ttcaagaaat gatgcaatct ggccaaatgc caagtcaaga agaacaagat gaaatgtcta
     9661 aacttgggga aaaaattgaa tccaatgacc ttttaaaagt ttattttgac caacaacaac
     9721 ggttgtctgt ctatatgtct gatatcgaaa aaattgtctt tgcacccatg caggacttga
     9781 tgtaatagta taaaagagag agctccttga gttttctctt ttatgctatt tacattcaga
     9841 gcatcctaat tgctgcgctt ttacattatt gcattatcct ataaaaatgt atttttatac
     9901 tttttttgag agcggctatt tatttttaga cttagtcatg tctctaaata cccctagtta
     9961 gtgataaaaa caagg
//
LOCUS       DATROD010000062         9955 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.062, whole genome shotgun sequence.
ACCESSION   DATROD010000062 DATROD010000000
VERSION     DATROD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.062"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            53..1471
                     /locus_tag="VBC74_001377"
     CDS             53..1471
                     /locus_tag="VBC74_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEO6942321.1"
                     /translation="MVRVQEEKILKNTGYALSRNPQVIQTLKDNHYNQSLQKQMLFLS
                     KKSNLDYIVLINLKGIRFTHPDSTKIGKPFQGGDEQAVFKGKAIMSTAEGSLGKSLRY
                     LIPVYDHQKQVGAIAVGLKLTTLGDLSQSSIKEFSKPLLISILISLVVTSIISYGLKK
                     QLHNLHPSDIFQHLEERNATLDQIQAAVFVIDQRHIIKRNNPAASLLFKKEGQRDLFS
                     GKLLESLIPQLKQDHFSKKTEQVLHFQGQDYLLSISPITVKTQNRGYVVFLRNVTETL
                     FTLDQLAHTTAYASALQAQTHQFMNQLHVIYGLADIEYYDELKIYLKELLEPQNEFLA
                     RLSMLVREPRLASFIIGEREKFAEKHINLSTEILVEIPTKSTVEDVNNYLLLHRYINT
                     KILTLLNSTTLVSLRLNYQNNLIETDYQWENEKWLLNDYHQYFNDAYFQQLLVDSRAT
                     YKISLKQNKVIIRTNIIYSEGS"
     gene            1473..2153
                     /locus_tag="VBC74_001378"
     CDS             1473..2153
                     /locus_tag="VBC74_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEO6942322.1"
                     /translation="MDVLIIEDDPMVEFIHRNYLEKLNYFQNIYSTASQTQAIAYLND
                     IKIQLVLLDIHIKEGNGLELLKLLRNQHQNTEVIVISAANEAHTVKEAFHLGIVDYLI
                     KPFTFERFESSIEKFLNHYHTFEADKIYQDNIDHFQKIDSGWLEGEVKLDEKGLSEIT
                     YQHILDAIQELEQPFTIQELAKCSQFSHVSVRKYIAYMEEKGLLTSQQIYTKVGRPYK
                     VYKLIKKT"
     gene            complement(2174..3169)
                     /gene="galE"
                     /locus_tag="VBC74_001379"
     CDS             complement(2174..3169)
                     /gene="galE"
                     /locus_tag="VBC74_001379"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEO6942323.1"
                     /translation="MAVLILGGAGYIGSHMVDQLITQGKEKVIVVDNLVTGHRQAVHS
                     DAIFYEGDLSDKTFMRQVFRENPDVDAVIHFAAFSLVAESMENPLKYFDNNTAGMIKL
                     LEVMNECDIKNIVFSSTAATYGIPEQVPILETAPQNPINPYGESKLMMETIMKWADQA
                     YGIKFVALRYFNVAGDKPDGSIGEDHKPETHLLPIILQVAQGVRDKIMIFGDDYNTPD
                     GTNVRDYVHPFDLADAHILAVDYLRQGNESNVFNLGSSTGFSNLQMLEAARRITGKEI
                     PAQKAARRPGDPDTLIASSEKARQMLGWEPKFDNIDKIISSAWAWHSSHPNGYED"
     gene            complement(3257..4864)
                     /locus_tag="VBC74_001380"
     CDS             complement(3257..4864)
                     /locus_tag="VBC74_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEO6942324.1"
                     /translation="MKKHWWHKATIYQIYPRSFMDSDGDGVGDIKGITSKLDYLEKLG
                     ITAIWLSPVYQSPMDDNGYDISDYQAIADIFGDMNDMDQLLQEANQRGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDKASGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIDKGIGGFRMDVIDLIGKIPDKGITGNGPKLHDYL
                     KEMNRASFGKHDLLTVGETWGATPDIAKQYSNPDNEELSMVFQFEHVGLQHKPDAPKW
                     DYSDGLDVPALKDIFTKWQTQLELGQGWNSLFWNNHDLPRVLSIWGNDSDNRKQSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFECLADVDDIESLNYAKEAMDNGVSEATILDS
                     IRKVGRDNARTPMQWSQGHQAGFTNGTPWLAVNPNYQEINVEAALNDTESIFYTYQKL
                     VALRKEHDWLVDADFKLLETADKVFAYVRQTDKERYLIVANLSDQNQSFEFPEAVKET
                     IISNTEVQEVLSSNTLKPWDAFCIELF"
     gene            complement(4993..6126)
                     /gene="ugpC"
                     /locus_tag="VBC74_001381"
     CDS             complement(4993..6126)
                     /gene="ugpC"
                     /locus_tag="VBC74_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEO6942325.1"
                     /translation="MVELNLNHIYKKYPSASHYSVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIDGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKFSKQEIDKRVREAAANIGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHQRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPDGD
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPSMNFFKVKVENGMIISEDGLRIAIPEG
                     QEKLLESRGYKGKELIFGIRPEDISSNLLVQDTYPNANVEAEVLVSELLGSETMLYVK
                     LGQTEFASRVEARDFHNPGEKVNLTFNVAKGHFFDADTEQAIR"
     gene            complement(6227..7078)
                     /locus_tag="VBC74_001382"
     CDS             complement(6227..7078)
                     /locus_tag="VBC74_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6942326.1"
                     /translation="MDFKELFPEVITKQEVKQSEDYIIVEQDGHVLHFPKSSLTKREL
                     CLLQMTQSLEEASSVDSQNPWYRYLVEGRGRLPQSYSAVQFIFIEHQFTLSEELKDFL
                     SPLVINVETIMTINQTQSVMILNQDNFFNATELLTDILPTIENDFNTRLRCYFGNSWT
                     HLQAVDWKELYEEEYKLFTLFLSHKAEQHYCRFPKMALWALANQSPMPSIKAKCLQHI
                     LDTSDTSAIIKALWQEQGNLAKTAKALFIHRNSLQYKLDKFTQSSGLNLKILDDLAYA
                     YLISMDT"
     gene            complement(7173..8069)
                     /locus_tag="VBC74_001383"
     CDS             complement(7173..8069)
                     /locus_tag="VBC74_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEO6942327.1"
                     /translation="MAITIQNHELQVTLKALGATMTSITDSQGVEYLWQGEPTYWGGQ
                     APILFPICGSVRNDCVIYRPAQAPHFTGIIPRHGFVRHKTFDYDYISDSSVRFTIKSS
                     EETLINYPYRFSLEITYTLRNKSIAITYIVKNLESEKNMPYAIGAHPGFNCPLFEKEV
                     FSDYYLEFEQFETCTIPESFPDTGLLDLQARHPFLENQKQLSLNYALFEKDAITLDQL
                     RSKTVYLKSRNHAKGIQLDFDDFENLILWTSNNGGPFLALEPWSSLSTSIEESDILED
                     KQNIVRLNPKQSKQHSIRITIL"
     gene            complement(8123..9100)
                     /gene="lacD"
                     /locus_tag="VBC74_001384"
     CDS             complement(8123..9100)
                     /gene="lacD"
                     /locus_tag="VBC74_001384"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEO6942328.1"
                     /translation="MGLTEQKQKHMEQLSDKNGIISALAFDQRGALKRLMAKYQSEEP
                     TVSQIEALKVLVAEELTPYASSMLLDPEYGLPATKVLDDNAGLLLAYEKTGYDTSSTK
                     RLPDCLDIWSAKRIKEEGADAVKFLLYYDVDSSDEVNEEKEAYIERIGSECVAEDIPF
                     FLEILSYDEKITDSSGIEYAKIKPRKVIEAMKVFSNPRFNIDVLKVEVPVNMDYVEGF
                     AQGETAYNKATAAAYFREQDQATLLPYIFLSAGVPAQLFQETLVFAKEAGAKFNGVLC
                     GRATWAGSVKEYVEKGEAGARQWLRTIGFQNIDELNKILQKTATSWKER"
     gene            complement(9102..>9955)
                     /locus_tag="VBC74_001385"
     CDS             complement(9102..>9955)
                     /locus_tag="VBC74_001385"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEO6942329.1"
                     /translation="VTDVSKTPGGKGLNVTRVLSQLGDNVVATGLLGGHFGDFIRSGL
                     DALEIRHQFLSIGGETRHCIAVLHEGQQTEILEKGPHITKDEAGAFLNHLKLIFDAAT
                     IITVSGSLPKGLPSDYYARLISLANHFNKKVVLDCSGEALRSVLKSSAKPTVIKPNLE
                     ELTQLIGRPISYSLDELKSTLQQDIFRGIDWVIVSLGARGAFAKHGNHYYQVTIPKIE
                     VINPVGSGDATVAGIASALEHQLDDINLLKRANVLGMLNAQETLTGHINLTYYQELIS
                     QIQVKEV"
BASE COUNT         3160 a         1976 c         1609 g         3210 t
ORIGIN      
        1 catttgtata ttttctattt tttattatgt cactatacat cagtcctatc gtatggtaag
       61 ggtacaggaa gaaaaaatct taaaaaatac tggttatgct ttatcgagaa atccacaagt
      121 tatacaaaca ctcaaagata atcactataa tcaaagtctc caaaaacaaa tgctattcct
      181 tagcaaaaaa tcaaatttag actatattgt ccttataaat ttaaaaggga tccgttttac
      241 ccaccctgac tccactaaga ttggtaaacc atttcaaggc ggtgatgaac aagctgtttt
      301 taagggaaaa gctattatgt ctactgcaga aggttcttta ggaaaatctt tacgatactt
      361 aattccagta tatgatcacc aaaaacaggt tggggcaatt gcagttggct taaaattaac
      421 gacacttgga gacttatctc aaagcagcat aaaagagttt agtaagcctc tcttgattag
      481 tattttgatt agcttagtcg tcacaagtat aatttcttat ggcttaaaaa aacaacttca
      541 caacctccat ccaagtgata tttttcaaca tttagaagag cgcaatgcaa cactggacca
      601 aattcaagcc gctgtatttg ttattgacca gaggcatatc atcaaaagaa ataatcctgc
      661 agctagttta ttattcaaaa aagaggggca acgagaccta ttttcaggta aattattaga
      721 atctttaatt cctcaactca agcaggatca tttttcaaaa aaaactgaac aagtattaca
      781 ttttcaagga caagactatt tactatctat ctcgccaatt acggttaaaa cacaaaatag
      841 aggctatgtt gtcttcttaa ggaatgttac tgaaacactt ttcactttgg atcaattagc
      901 acacactaca gcttatgctt ctgctctaca ggcccaaaca catcaattta tgaatcaact
      961 tcatgttatt tatggacttg cagacatcga atactacgac gagttgaaaa tttatttaaa
     1021 agaattattg gagccacaaa atgaattttt agcacgctta tcaatgttag ttagagagcc
     1081 tcgactggct agtttcatta ttggcgaaag agaaaaattc gcagaaaaac acatcaatct
     1141 atccacagaa attttagtag aaatccctac caagtcaact gttgaggatg ttaataacta
     1201 tctcttgctc cacagatata ttaatacgaa gatactcacg ctattaaatt caacgacttt
     1261 agtttcttta cgtttaaatt atcaaaataa cctcattgaa acagattatc aatgggagaa
     1321 tgagaagtgg ttactcaatg actaccatca gtatttcaac gacgcttatt ttcaacagtt
     1381 acttgtagat tcaagagcta cttataagat ctctttaaaa caaaataagg ttattataag
     1441 aacaaatatt atctactcag aagggtctta gtatggacgt attaattatc gaagatgatc
     1501 ctatggtgga atttatccac cgcaattacc ttgaaaaact caattatttt caaaacatct
     1561 attcaactgc tagtcaaaca caagctattg cttatcttaa tgacattaaa atccaacttg
     1621 ttcttttaga tattcatatt aaagaaggca atggtctaga attattaaag ttattacgca
     1681 atcaacacca aaataccgaa gtcattgtta tttcagcagc caacgaggcg cacactgtca
     1741 aagaagcatt tcatttaggt attgtagatt acctgataaa accatttaca tttgaacgct
     1801 ttgagtctag tattgaaaaa ttcctaaatc attatcatac atttgaagct gataaaatct
     1861 atcaagacaa catcgatcac tttcaaaaaa tagattctgg atggctagag ggagaggtta
     1921 aacttgatga gaaagggctc tctgaaatta cctatcagca tattcttgat gccattcaag
     1981 agttagagca gcctttcact atccaagagt tagcaaaatg cagtcaattt tctcatgtct
     2041 ctgttaggaa atatattgcc tatatggaag aaaaaggcct tctgacaagt cagcaaattt
     2101 atactaaggt cggtcgtcct tacaaagttt ataagcttat aaaaaaaact taggcctgta
     2161 gacctaagtt tttttaatct tcgtagccat ttggatgact agaatgccat gcccatgccg
     2221 atgaaataat tttatcaata ttatcaaatt taggctccca cccaagcatt tgacgagctt
     2281 tctctgagga agcaataagc gtatctggat ctcctggacg acgagctgcc ttttgagcag
     2341 gaatttcttt cccagtaata cgacgagccg cttctaacat ctgaaggtta gaaaaacctg
     2401 tcgaagatcc gagattaaac acgtttgatt cattcccttg gcgaaggtaa tcaactgcta
     2461 atatatgagc atctgccaaa tcaaatggat gcacataatc tcgaacatta gttccatctg
     2521 gagtattgta atcatctcca aaaatcatta ttttgtcacg tactccttga gcaacttgaa
     2581 gaatgattgg taacaaatgt gtttctggtt tatgatcttc cccaattgag ccatccggtt
     2641 tatctccagc aacgttgaag taacgtagag cgacaaactt aataccgtag gcttgatcag
     2701 cccatttcat aattgtttcc atcataagct tactttcgcc ataaggatta ataggatttt
     2761 gaggagctgt ttctaaaata ggaacttgtt ctggaatacc atatgttgca gctgttgatg
     2821 aaaagacaat atttttgata tcacattcat tcattacttc taaaagtttt atcattccag
     2881 cagtgttatt atcaaaatat tttaaaggat tttccattga ctcagcaact agtgaaaatg
     2941 ccgcaaaatg gataacagca tctacatcag gattttccct aaaaacttga cgcataaacg
     3001 ttttatcgga taaatctcct tcataaaaaa tcgcatctga atgcaccgct tgacgatgac
     3061 ctgttaccaa gttatcaaca acaataactt tctcttttcc ttgtgtgatt aactgatcaa
     3121 ccatatgcga gccaatgtaa ccggcgccac ctaaaatcag aactgccata tttttctcct
     3181 atttcaatac tcttttaaca tcatttgtta cctcattata ctgagaataa gctttttttg
     3241 caaatatcta ccgaaattag aatagctcta tgcagaaggc atcccacggc ttgagagtgt
     3301 tacttgatag tacttcttga acctctgtat ttgaaataat agtttcttta acagcttctg
     3361 gaaattcaaa agattggttt tgatcggata agtttgcaac tattaaataa cgttctttat
     3421 ctgtttgacg aacataagca aataccttat ctgctgtttc caaaagttta aaatcagcat
     3481 caactaacca gtcgtgttct ttacgtaaag caactaattt ttgataagta taaaaaatac
     3541 tttccgtatc atttaacgct gcctccacat taatttcctg ataattggga ttgactgcta
     3601 gccatggggt accgttagta aaaccagctt gatgtccttg actccactgc attggagtac
     3661 gagcattatc acgaccgact ttacgaatac tatctaaaat tgttgcttca gataccccat
     3721 tatccatggc ttctttagca tagttaaggg attcaatatc atctacatct gctaaacact
     3781 caaatggata attggtcata ccaatttctt caccttgata gatgtaaggc gttccacgca
     3841 tcaaatgtag taggattgct agagccttgg cagattgttt tctgttatca ctatcatttc
     3901 cccaaatcga gagcacccga ggaaggtcgt gattattcca gaataaagaa ttccaacctt
     3961 ggccaagctc caattgtgtt tgccattttg taaaaatatc tttcaatgct ggaacatcta
     4021 atccgtcact gtaatcccac ttaggagcgt caggtttatg ttgaagacct acatgttcaa
     4081 attgaaagac catagataat tcttcattat cgggatttga gtattgtttt gcaatgtctg
     4141 gcgttgctcc ccaagtttca cccaccgtca ataagtcatg ctttccaaaa ctagctctgt
     4201 tcatttcttt aagataatca tggagttttg gtccattacc agtaatgccc ttatctggaa
     4261 ttttcccaat taaatcaatg acatccatac ggaaaccacc aataccctta tcaatccaga
     4321 aattcatcat atcataaatt ttatgacgta aagcttcatt ctcccagttg agatctggct
     4381 gacgtttgct aaaaaggtgc aaataatatt gacctgatgc tttatcatat tcccaagctg
     4441 agccactaaa aatagaagtc aaatcgttag gttcatctcg ccaaatataa aaatcacgtt
     4501 caggactatt agggttttct cgcgcttcta cgaaccaagc atgttcatca gaggtatgat
     4561 tcactaccaa atccatgatg attttaatcc cgcgttgatt tgcctcttgt aacagttggt
     4621 ccatatcatt catgtcaccg aaaatatctg caatggcttg ataatctgaa atatcataac
     4681 cattgtcatc cattggactt tggtaaacag gtgataacca gatagcagta atccctaatt
     4741 tttcaaggta gtctagtttg ctagtaatcc ctttaatgtc ccccacacca tcaccatctg
     4801 aatccataaa tgaacgtggg taaatttgat aaatcgtagc tttatgccac cagtgtttct
     4861 tcattattct tctcctatca ataattcatt catcttttat tttaacgttt ttcctctttt
     4921 agtgcaatcg tttgcgcaaa taacattata aaaaggctgg aacccaacct ttttatgaat
     4981 atcgcttata ccttaacgaa tagcttgttc agtatctgca tcaaagaaat gccccttagc
     5041 aacattaaac gttaggttaa ctttttctcc tggattatgg aagtcacgcg cttcaacacg
     5101 agaagcaaat tctgtttgac ctaacttaac ataaagcata gtttcgctac cgagtaattc
     5161 agaaactaaa acttctgctt caacattggc atttggataa gtatcttgga caagaagatt
     5221 gcttgaaatg tcttctggac gaataccaaa aatcaattct tttcctttat agcctcgtga
     5281 ttctaaaagt ttttcttgac cttcagggat agcgattcga agaccatctt cactaataat
     5341 cataccattt tcaactttaa ctttgaagaa gttcatagat ggactaccga tgaatcccgc
     5401 aacaaattta ttagcaggaa gattatagag ttcttgtggt gaaccaactt gctcaatttt
     5461 accaattgta ccatcaccat cagggttctt agtagctgac ataatgacaa tacggtctgc
     5521 caaagtcatc gcctctgttt ggtcgtgggt aacataaata gttgttgaac caatacgttg
     5581 atgaattttt gcaatttcag cacgcattga aacacgtaat ttagcatcta agttagataa
     5641 aggctcatcc attaagaaca ctttggcatc acgcacaata gcacgtccca tagcaacacg
     5701 ttggcgttgt ccacctgaca aatccgcagg tttgcgctct aagaattcag taaggccaat
     5761 attagctgct gcttcacgaa cgcgcttatc aatttcttgt tttgagaatt tacgcaattt
     5821 gagcccaaaa gccatattat cgtaaacagt catatgtgga taaagagcat agttttggaa
     5881 aaccatagca atatcacgat ctttaggtga tttgtcattg acaacttcgc catcaatctt
     5941 gagctcacct tcactaatat cttcaagacc tgcgatcata cgaagtgttg ttgatttccc
     6001 acaacctgat ggtccaacaa aaacaataaa ttctttgtct ttaatatcca agtcaaagtc
     6061 ttcaacagaa taatgcgatg cacttggata ttttttgtaa atatgattta aatttaattc
     6121 taccattaga ttgacctctt ttattattgt gattttatta tagcaaagaa agggttttca
     6181 tataatggta accttgccta aatttcttgt gcaaactaac taaattttag gtatccatcg
     6241 aaattaaata ggcataggct aaatcatcta atatttttaa attaagcccc gaagattggg
     6301 taaatttatc taatttatat tgcaatgaat tccgatgaat gaaaagtgcc ttagccgttt
     6361 tagcaaggtt cccttgctct tgccacaagg cttttattat agccgaagta tcactcgtat
     6421 caaggatatg ttgtaaacat tttgctttaa tagaaggcat gggcgattga tttgccaaag
     6481 cccataaagc catctttgga aatcgacagt aatgttgttc ggccttgtga ctaagaaaaa
     6541 gcgtaaaaag tttatattct tcttcatata attctttcca gtccactgct tgtagatgcg
     6601 tccaactatt tccaaaatag catcttaaac gcgtgttaaa gtcattttca atcgttggta
     6661 aaatatctgt tagtaattcc gtagcattaa agaaattatc ctgatttaaa atcatgacac
     6721 tttgggtttg attaattgtc ataattgttt ctacgtttat tactaaaggc gacaagaaat
     6781 ccttaagttc ttctgataag gtaaattgat gctcaataaa aataaattgg actgccgaat
     6841 aagattgggg caaccttcca cgaccttcaa ctagatagcg gtaccaagga ttctgagaat
     6901 ccactgaact cgcctcttcc aggctttgtg tcatttgtaa taagcatagt tcacgcttag
     6961 ttagcgaaga ttttggaaag tggaggacat gaccatcttg ctcaacgatg atataatcct
     7021 cactctgttt aacttcctgt ttagtaataa cttctggaaa cagttcctta aaatccatgg
     7081 catcctccac tacagttttg ttcattatat catatttata acctcttttt gagcacagaa
     7141 aaagaggaga atgtctcctc agaaagtttc tgttataaaa tagtgatccg aatagaatgc
     7201 tgtttcgact gctttggatt aagccgtacg atattttgtt tatcttctaa aatatcagac
     7261 tcttcaatag aagttgacaa ggaactccag ggttccaaag ccaaaaaagg accaccattg
     7321 ttacttgtcc aaagaataag attctcaaaa tcatcaaagt caagttggat tccttttgca
     7381 tgatttctag attttaagta gaccgtctta gatcgtagtt ggtctaaagt aatagcatct
     7441 ttttcaaata aagcataatt aagagataat tgtttttgat tttctaaaaa aggatgtcta
     7501 gcttgtaaat ctaacaagcc tgtatccgga aacgactcag gaattgtgca agtttcaaac
     7561 tgttcaaact ctagatagta atcactaaat acttcctttt caaacaaagg gcaattaaag
     7621 cctgggtgag ctccgatagc atatggcata tttttttctg attctaagtt tttgacaata
     7681 tatgtaatgg caatgctttt atttcttaag gtgtaagtta tttctaaact aaaacgataa
     7741 ggataattta tcaacgtttc ttcactactt ttaattgtaa aacgaacaga agaatccgat
     7801 atgtaatcat agtcgaatgt tttgtgacgt acaaagccat gccttggtat gatacccgta
     7861 aaatgtggag cttgagctgg tctgtaaatg acgcaatcat ttcgaacact tccgcatata
     7921 ggaaataaaa taggagcttg cccaccccaa taagtgggtt ccccttgcca aaggtactca
     7981 acgccttgac tatctgtaat agaagtcatt gttgcaccta acgcttttaa tgtcacttgc
     8041 aactcatgat tttgtatcgt aatagccatt atttttcctt tcataaagag caataattgc
     8101 tttaccaact tttctctagc aattatcttt ccttccaaga agttgctgtt ttttgcaaaa
     8161 ttttatttag ttcatcaata ttttgaaaac ctattgtacg taaccattga cgtgcaccag
     8221 cttctccttt ttcaacatac tctttaacac ttccagccca agttgctcta ccacaaagga
     8281 caccgttaaa tttagcacca gcttctttag caaacacaag agtctcttga aaaagctgag
     8341 caggaactcc tgcactcaaa aaaatgtagg ggagtagtgt cgcctgatct tgttctctaa
     8401 aataagccgc agctgtcgct ttattataag cagtctctcc ttgcgcaaaa ccttctacat
     8461 agtccatatt gacaggcact tccaccttca agacatcaat attaaatcga ggatttgaaa
     8521 aaactttcat agcttcaata accttacgtg gcttaatttt ggcatactca atacctgagc
     8581 tatccgtaat cttttcatca tatgacaaaa tttcaaggaa aaaaggaatg tcttctgcaa
     8641 cacattcaga accaatccgt tcaatataag cttctttttc ttcgtttact tcatccgaac
     8701 tatcaacatc ataataaagt aagaacttaa ctgcgtctgc cccctcttct ttaatgcgtt
     8761 tcgcagacca aatgtctaaa cagtctggga gtcgcttggt actactggta tcataacctg
     8821 ttttttcgta cgcaagtaaa agaccggcat tgtcatctaa aactttcgta gctggcaacc
     8881 catattctgg atctaacaac attgatgaag cgtaaggggt taattcttca gctaccaaaa
     8941 ctttaagtgc ttctatttga gaaacagtgg gctcctcaga ttgatatttt gccattaatc
     9001 gcttcaatgc tccacgttgg tcaaaagcca aggccgaaat aataccattt ttatcactca
     9061 attgttccat atgtttttgt ttttgttccg ttaatcccat cctatacctc tttcacttgg
     9121 atttgactga ttaattcttg atagtaggtc agattgatat gccctgttaa tgtttcttgt
     9181 gcattaagca tacccaagac attagctctc ttaagtaaat ttatatcatc tagttgatgt
     9241 tctaaagcag acgcgattcc tgcaaccgtt gcatcccctg aaccaactgg attgataact
     9301 tctatttttg gaatcgtcac ttgatagtag tgattcccat gtttagcaaa agctccccta
     9361 gcacctaagg aaacaataac ccaatcaatt cctctaaaaa tatcttgctg taaggtagat
     9421 tttaattcgt ctaaactata gctaattggc cttccaatta attgtgttaa ttcttcaagg
     9481 ttaggtttaa tgacggtcgg cttagcacta gattttaaaa ccgaacgtaa tgcttctcct
     9541 gaacagtcta aaacaacttt cttattaaag tgattcgcta gggagattaa cctagcataa
     9601 taatctgagg ggagtccctt tggtaaactt cctgatactg ttatgatagt tgcagcatca
     9661 aagataagtt ttagatgatt caaaaaggcg cctgcttcat ctttagtaat atgtggacct
     9721 ttttctagaa tttcagtctg ctgaccctca tgtaaaactg caatacaatg acgtgtttct
     9781 ccaccaatac ttaaaaattg gtgtctgatt tccaatgcat ctaagccact tcggatgaaa
     9841 tctccaaaat gcccccctaa tagaccagtt gccactacat tatcgccaag ttgagaaaga
     9901 acacgagtca catttaatcc cttaccacca ggtgttttgg agacgtcggt aaccc
//
LOCUS       DATROD010000063         9911 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.063, whole genome shotgun sequence.
ACCESSION   DATROD010000063 DATROD010000000
VERSION     DATROD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9911
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.063"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            149..1894
                     /locus_tag="VBC74_001386"
     CDS             149..1894
                     /locus_tag="VBC74_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6942330.1"
                     /translation="MSIIKNLWWFFKEEKKRYLIGILSLSLVAVLNLIPPKIMGSVID
                     AITTGKLTRPQLLWNLLGLVLSALAMYGLRYIWRMYILGTSYKLGQVVRYRLFEHFTK
                     MSPSFYQKYRTGDLMAHATNDINSLTRLAGGGVMSAVDASITALVTLITMFFTISWQM
                     TLIAVIPLPLMALATSKLGRKTHETFKESQAAFSELNNKVQESVSGVKVTKSFGYQEQ
                     EIASFQEINQMTFVKNMRTMTYDVMFDPLVLLFIGASYVLTLAMGAFMISKGQVTVGD
                     LVTFVTYLDMLVWPLMAIGFLFNMVQRGSVSYNRINSLLEQESDITDPLNPIKPVVNG
                     TLRYDIDFFRYDNEETLADIHFTLEKGQTLGLVGQTGSGKTSLIKLLLREHDVTQGKI
                     TLNKHDIRDYRLSELRQLIGYVPQDQFLFATSILENVRFGNPTLSINAVKEATKLAHV
                     YDDIEQMPAGFETLIGEKGVSLSGGQKQRIAMSRAMILDPDILILDDSLSAVDAKTEH
                     AIVENLKTNRQGKSTIISAHRLSAVVHADLILVMRDGRVIERGQHQELLNKGGWYAET
                     YASQQLEMEEAFDEV"
     gene            1884..3623
                     /locus_tag="VBC74_001387"
     CDS             1884..3623
                     /locus_tag="VBC74_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6942331.1"
                     /translation="MKSNQWQVFKRLISYLRPYKWFTVLALSLLLLTTVVKNIIPLIA
                     SHFIDHYLTNVNQTAVLILVGYYSMYVLQTLIQYFGNLFFARVSYSIVRDIRRDAFAN
                     MERLGMSYFDRTPAGSIVSRITNDTEAISDMFSGILSSFISAIFIFTVTLYTMLMLDI
                     KLTGLVALLLPVIFILVNVYRKKSVTVIAKTRSLLSDINSKLSESIEGIRIVQAFGQE
                     ERLKTEFEEINKEHVVYANRSMALDSLFLRPAMSLLKLLAYAVLMSYFGFTGVKGGLT
                     AGLMYAFIQYVNRLFDPLIEVTQNFSTLQTSMVSAGRVFDLIDETGFEPSQKNTEAFV
                     REGNIEFKNVSFSYDGKKQILDNVSFSVKKGETIAFVGATGSGKSSIINVFMRFYEFQ
                     SGQVLLDGKDIRDYSQEQLRKNIGLVLQDPFLYHGTIKSNIKMYQDITDQEVQDAAEF
                     VDADQFIQKLPDKYDSAVSERGSSFSTGQRQLLAFARTVASKPKILILDEATANIDSE
                     TEQIVQDSLAKMRQGRTTIAIAHRLSTIQDANCIYVLDRGKIIESGNHESLLDQKGTY
                     YRMYQLQAGMMEV"
     gene            3751..4317
                     /locus_tag="VBC74_001388"
     CDS             3751..4317
                     /locus_tag="VBC74_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEO6942332.1"
                     /translation="MLLLVDNYDSFTYNLKQYLSVYKEIFVIKNDDPNLFLLAESAEA
                     IVLSPGPGHPKDAGKILELINQFIGQKPILGICLGHQALAECLGGRLNLANHVMHGKQ
                     SWVTINDHTSLFKGIDSPTQVMRYHSLVVTDLPENIAIIARSNEDNEIMAFHCPSLKV
                     YAMQFHPESIGSIDGMKMIENFLTLIND"
     gene            complement(4385..4923)
                     /locus_tag="VBC74_001389"
                     /pseudo
     CDS             complement(4385..4923)
                     /locus_tag="VBC74_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857893.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
     gene            complement(4924..5916)
                     /gene="bioB"
                     /locus_tag="VBC74_001390"
     CDS             complement(4924..5916)
                     /gene="bioB"
                     /locus_tag="VBC74_001390"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEO6942333.1"
                     /translation="MSFQTNYIHLADEILSGKTSISYEQALEILNSDENWWEIYAAAL
                     YLKNQVSRNNIRLNVLLSAKQGLCAENCGYCSQSKESTADIDKFGLLPQNVILKQAIV
                     AHQNGASVFCIAMSGTKPSKREIEQLCQVIPEIKKSLPLEICLTAGFLDREQLHQLKQ
                     AGIDRINHNLNTPEENYPNIATTHSFKDRCDTLERIHNEDIDVCSGFICGMGESDEGL
                     ITLAFRLKELNPYSIPVNFLLAVEGTPLGKYNYLTPIKCLKIMAMLRFVFPFKELRLS
                     AGREVHFENFESLVTLLVDSTFLGNYLTEGGRNQHTDIEFLEKLQLNHTKKELI"
     gene            6041..6556
                     /locus_tag="VBC74_001391"
     CDS             6041..6556
                     /locus_tag="VBC74_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942334.1"
                     /translation="MKKLFKKAIAMCRDLPLNFVIVELSLYQREDGSSDNIKWISKRI
                     LEVTVTDQQWIYHLRMLNLPKLKPMSLASSDCPAREEKASLISIEECQKFTSLVRDKN
                     PFHQGEAAIVPAALLLEKIMEVRNGKIKEIHLTFLSPVRVNQEFSVQVVDNTVIIFQH
                     QILCVRGTYEE"
     gene            6546..7661
                     /locus_tag="VBC74_001392"
     CDS             6546..7661
                     /locus_tag="VBC74_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEO6942335.1"
                     /translation="MKNRDVYIGFGLRTPIGIKGKQFKHYRPELLGAHLLNQIKKIES
                     ESNIDSIICGNTVGTGGNIGRLMTLFSDYESYIPVQTIDMQCASSSSALFFGYLKISA
                     GINEKVLVGGIESSSLQPMRRYAKEDNRNGEYTVAQFSPDSYAETVMLEGAQRVCQKY
                     GFRREMLDKLAFLSHKRALTAKQGGYLEEVILPMEGMRDQGVRKLKEAFFQKLPRLME
                     NSPLLTIGNVCLMHDAAAFLTLQSQKTEFRIVHIVEVAGDPKLSPELVHTATEKLLTE
                     THTKISDYDAIEWNEPFAAIDALFNHYYPEEREKFNIFGGALAYGHPYACSGIINILH
                     LMQALKYKNKPMGLTAIAGAGGVGMAISIEYLGVKNA"
     gene            7654..8883
                     /locus_tag="VBC74_001393"
     CDS             7654..8883
                     /locus_tag="VBC74_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEO6942336.1"
                     /translation="MLESLKTIVKTNSDKKLFDGDLQVSYGEFYNLVRQDMASQDNRK
                     HVISTHSLLNQLVRFVSKLCQKALPIICKPNLTHNEISRLEKEVQYAPQLADFGVLSS
                     GTTADAKLLWRSFASWSDFFSIQNAYFSVTSNSRLFIQGDFSFTGNLNLALSLLLLGG
                     TLVVTQKNSVKYWQTLWEKTGVTHLYLLPSYLKLVEQYSKETALDNKTIITSSQYVSD
                     SLLEGLYIKHPKVSVKIFYGASELNYVSWYDGRDIRDKPQYVGEIVPNVAVRIKEGRI
                     FVKTPYSICGLSSEYCAGDYGELIDGKLYLFGRGDDWCNQSGIKLYLPRLIEKIKTCP
                     YIKDAVAFTKESQSHGQESHCCIVLIENQMQQECLKWLSEHFEKKYGFKHYHIVSKIP
                     LMPSGKIDYQQLKRQLA"
     gene            8996..9628
                     /gene="nth"
                     /locus_tag="VBC74_001394"
     CDS             8996..9628
                     /gene="nth"
                     /locus_tag="VBC74_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEO6942337.1"
                     /translation="MLSKAKSRYIIREIIKLFPDAKPSLDFTNVFELLVAVMLSAQTT
                     DAAVNKVTPALFERFPNPLVLAQADPKEIEPYISKIGLYRNKARFLNQCAKQLIEHFD
                     GKVPQTRQELESLSGVGRKTANVVMSVGFGIPAFAVDTHVTRICKHHQICKQSASPLE
                     IEKRVMEVLPPEEWLAAHQSMIYFGRAICHPKNPKCDQYPQLYHFPDNLK"
     gene            complement(9629..>9911)
                     /locus_tag="VBC74_001395"
     CDS             complement(9629..>9911)
                     /locus_tag="VBC74_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001884316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEO6942338.1"
                     /translation="TIILTPITKVSIVLCLFGILAEVVDINIGSGDFLYLATIGLSLP
                     LHQMLFIIQIGAWLGIIYCLVMRKMKKTIAFLPFLSIAYIIVTSYSLLF"
BASE COUNT         3225 a         1596 c         1945 g         3145 t
ORIGIN      
        1 aaggatcact ttagttgacg aaagtaagct tatagaggca taatagagag ttgttaaatg
       61 agaattttaa aacacttttt atgtcttctg attgatttgg tcttctgact taattatgat
      121 gtttttcaac ctttgagaaa ggaaaaatat gtctattata aaaaatttgt ggtggttttt
      181 taaggaagaa aagaagcgct atcttattgg gattttgtca ttaagtttgg ttgcagtgtt
      241 aaatcttatc cctcctaaaa tcatgggatc agttattgat gctattacaa ctggaaaatt
      301 aacaagacca caattactat ggaatttatt aggtttggtt ttgtcagctt tagctatgta
      361 tgggctgcgt tatatttggc gtatgtatat tttagggact tcttacaaat taggccaagt
      421 tgtcagatac cgtttatttg aacattttac aaaaatgtct ccttcttttt atcagaaata
      481 tcgtacaggt gatttaatgg cgcacgcgac caacgacatc aattctctaa cacgtcttgc
      541 aggaggagga gttatgtcag cagtggatgc ctctatcaca gcattagtaa cgcttatcac
      601 catgttcttt actatttcgt ggcaaatgac attaattgcg gttatccctt tgcccttaat
      661 ggccttagca actagtaaat tggggcgaaa aacccatgaa accttcaaag aatctcaggc
      721 agccttttca gaattaaata ataaagtgca agaaagtgtc tctggcgtca aagtgactaa
      781 atcatttggt tatcaagaac aagaaattgc ttcttttcaa gagattaatc agatgacttt
      841 cgtgaagaac atgcggacca tgacttatga tgtcatgttt gatcctttag ttcttctttt
      901 tataggtgcc tcctacgtat taacattggc tatgggagct tttatgattt caaaaggtca
      961 agttactgtt ggtgacttgg taacatttgt gacgtattta gatatgttgg tatggccctt
     1021 gatggcgatt ggtttcttgt tcaatatggt acagcgtggt agtgtttctt ataaccgtat
     1081 taatagtcta cttgagcaag aatcggatat aactgatcct ttaaatccta tcaaacctgt
     1141 tgtcaatgga acattaagat atgatattga tttctttaga tacgacaatg aggaaacctt
     1201 agccgatatt catttcacct tagaaaaagg tcaaacctta ggtttggtag gtcaaacggg
     1261 atcagggaag acaagtctta ttaagttatt gctacgtgaa catgatgtga ctcaggggaa
     1321 aattacttta aataaacatg atatacgtga ttatcgattg tctgagttac gtcaactaat
     1381 cggttatgtt cctcaagatc agtttttatt tgctaccagt attttagaaa atgttcgctt
     1441 tggaaatcca actctatcta tcaatgctgt caaagaagca actaaattgg cacatgttta
     1501 cgatgacatt gaacagatgc cagcaggatt tgagactcta attggagaaa aaggagtctc
     1561 attatctggt ggacaaaaac aaaggattgc gatgagtcgt gccatgattt tagatccaga
     1621 tattcttatt ttggatgatt ctctatcagc agtggacgct aaaacggaac atgctattgt
     1681 tgagaatctt aaaacgaatc gtcaagggaa atcgactatt atttcagcac atcgtttatc
     1741 agctgttgtg cacgcagacc ttatcttagt tatgcgagac ggcagagtca ttgagcgagg
     1801 tcaacatcaa gagttgctaa ataaaggtgg ttggtatgct gaaacgtatg cctcacagca
     1861 attagaaatg gaggaagcat ttgatgaagt ctaatcaatg gcaagtcttt aagagattaa
     1921 tctcctattt acgcccttat aaatggttta cagtattagc tctatctctc ttattgttga
     1981 cgactgttgt taaaaatatt attcctttaa ttgcttcaca ttttattgat cactatctga
     2041 caaatgttaa tcaaacagca gttcttattt tagtgggata ttattcaatg tatgtcttgc
     2101 agaccttaat tcaatatttt gggaatctct tttttgcgcg tgtttcttat agtattgtta
     2161 gagatattcg tagagatgct tttgctaata tggaaaggct aggcatgtct tattttgata
     2221 ggacaccggc aggatctatt gtgtcacgta ttactaatga tactgaagca atatctgata
     2281 tgttttcggg tattttatca agttttatct cggcgatatt tatttttaca gttactctgt
     2341 acactatgtt gatgctagac attaaactaa caggactcgt cgctcttttg ttacctgtta
     2401 tctttatatt agtgaatgtc tatcggaaaa aatcagtcac tgtcattgct aaaacgagaa
     2461 gtttacttag tgatatcaac agtaaattat cagaaagtat tgaaggaatt cgcattgtac
     2521 aggcttttgg tcaagaagag cgcttgaaga ctgaatttga ggaaattaac aaagagcatg
     2581 ttgtgtatgc caatcgttct atggctcttg atagtctctt cttaagaccg gcgatgtctc
     2641 ttttaaaact cctagcatat gctgttctta tgtcttattt tggatttaca ggagttaaag
     2701 gaggtcttac ggcaggatta atgtatgctt ttattcagta cgttaatcgt ctatttgacc
     2761 ctttaattga agtaacgcaa aatttttcaa ccttacaaac atcaatggta tcagcagggc
     2821 gtgtgtttga tctgattgat gaaacaggtt ttgaaccaag ccaaaaaaat acagaagctt
     2881 ttgtgagaga aggaaatatc gaatttaaaa atgtatcatt ctcatatgat ggaaaaaaac
     2941 aaatccttga taatgtttct tttagtgtta aaaaaggtga aactattgct tttgtaggag
     3001 cgacaggttc tggaaaatca tctattatta atgtctttat gaggttttac gaatttcaat
     3061 ctggacaagt tttattagat gggaaggata ttagggatta ctcacaagaa caattgcgaa
     3121 agaatattgg actcgtctta caagacccct ttttatatca tggcactatt aaatctaata
     3181 tcaaaatgta ccaagacatc accgatcaag aggtacagga cgcagcagag tttgttgatg
     3241 ctgatcaatt tattcagaag ttgccagata agtacgattc agctgtttca gaacgtgggt
     3301 caagtttttc aactggacaa cgccaactat tagcgtttgc cagaactgtg gctagtaaac
     3361 ctaaaatttt aatcttagat gaggcgacag caaatattga ttcggaaaca gagcagattg
     3421 tacaagattc gttagcaaaa atgcgtcaag gtcgaacaac aattgccatt gctcaccgct
     3481 tatcaaccat tcaagatgct aattgtatct atgttcttga tagaggaaag ataattgaaa
     3541 gtgggaatca cgagtcttta ttagaccaaa aagggactta ttatcgtatg taccaattac
     3601 aagccggcat gatggaggtc tagaaaattt aggctattca aaaggcataa tctgtgaggc
     3661 ttagaggttg gaatagttat tcctctaagc cttttttata ttttacaatt agtgtataat
     3721 gaggtcaaag tacaaggaaa ggtctataaa atgttactat tggtagataa ttacgattct
     3781 tttacctata atttaaagca atatttaagt gtctataaag aaatattcgt cataaaaaat
     3841 gatgatccta accttttttt attagctgag agcgcagaag ctatcgtctt atcacctgga
     3901 ccaggacacc caaaggatgc tgggaaaata ttagaattaa tcaatcagtt tataggacag
     3961 aaacctattc taggtatctg tttggggcat caagcgcttg ctgagtgctt gggtgggcga
     4021 ttaaatttag caaatcacgt tatgcatggt aaacaaagtt gggtaacaat aaacgaccat
     4081 actagtttgt ttaagggaat tgatagtcca acccaagtta tgcgttatca ctcattggta
     4141 gtgactgatt taccagagaa tattgctatt attgcacgat caaatgagga taatgaaatc
     4201 atggcattcc attgccccag cttaaaggta tatgcgatgc agtttcatcc tgagtcaatc
     4261 gggagtattg atggaatgaa aatgattgaa aactttttga cgttaattaa tgactaaaat
     4321 gtgttagtgt aggataatga ctctctataa ataaaagaca gtacctaagg gcactgtctt
     4381 ttatttagtt gtatagatga gttaatctgt ctgcaaattt acgataaata atcaagcaaa
     4441 tggatgcttt taaggtatcc ccaggaataa aaataaggtt agaaaatagt gatttagtta
     4501 aggggagaga agtttgaaag gaaagaacga tgctaccaca aatatcaatc aatagtacgc
     4561 caaatatcca aataataagg aattaaaccc agagttttgt tgttttcact ttctcaagcc
     4621 ctaggccgat aaaaaaagct gcaaatggat aagtcagcag atagcctgct gttggcccaa
     4681 acagcgttac gagacctgac cgccctcctg gtaaaatggt gcaccaatag caactaagag
     4741 caagaaaata gccacagcta ggaaaccttt cctactacca agcagagcac ctgccagcat
     4801 aacaccaaga ttttgtaaga caattgggac tggaatgaaa cctaatggga tccctgggat
     4861 aaaacctaaa acaatgagta gtgcaaccat gagtgcaata taggtagttg ttcttgtttt
     4921 catttaaatt aattcctttt tagtatgatt tagttgtaat ttttccaaga attcaatatc
     4981 ggtatgttga ttgcgacccc cctctgttag gtaatttccc aaaaaagttg agtcaacaag
     5041 taaggtgact aatgattcaa aattctcaaa atggacctcc cgtccagcac ttaatcttaa
     5101 ttccttgaaa ggaaaaacaa aacgcaacat ggccataatt tttaagcatt taatgggagt
     5161 caaatagtta tattttccaa gaggtgttcc ttcaacagca agtaaaaaat tgacagggat
     5221 agaatagggg ttcagttctt ttagtctgaa agctaatgtg atgagcccct catcgctctc
     5281 tcccatacca caaatgaatc cagaacaaac atcaatgtct tcattgtgaa ttctttctaa
     5341 agtatcacaa cgatctttga agctatgcgt cgttgcaatg ttggggtagt tttcttcggg
     5401 ggtgttgagg ttatgattga tacggtcaat tcctgcttgt tttagctgat gaagttgttc
     5461 tctatccaaa aaaccagcgg taaggcatat ttctagagga aggctttttt tgatttcagg
     5521 gatgacttga cataactgct caatttctct cttgctaggc ttagttccac tcatcgcaat
     5581 acaaaagacg ctagcaccat tttgatgagc gacaatagcc tgttttagaa tgacattttg
     5641 aggaagcaga ccaaatttat caatgtcagc agtgctttct ttagattgag aacagtaacc
     5701 acaattttct gcacaaagtc cttgcttagc acttaataaa acgttaaggc gaatattatt
     5761 tcggctaact tgatttttaa ggtataaggc agcagcgtag atttcccacc agttctcatc
     5821 gctatttaga atttccagcg cttgttcata agaaatgcta gtctttccag ataatatttc
     5881 gtcagccaaa tggatataat ttgtttgaaa tgacatgata gcctccttta acgaaattaa
     5941 taatagcata aaaaaactgt attgtaaact tagtctatat aataagttaa caatataaaa
     6001 aattatgata tactggtctt atattttgac gggagataag atgaaaaaac tatttaaaaa
     6061 agcgatagct atgtgccgag atttgccgct taattttgta attgttgaac ttagtttgta
     6121 tcaaagagag gatggctcat ctgataatat taaatggatt tctaagcgaa tccttgaggt
     6181 aacggtaact gatcagcaat ggatttacca tttgaggatg cttaatttac caaaacttaa
     6241 gccaatgtca ctagcatcca gtgactgtcc tgcaagggaa gagaaggcta gtcttatttc
     6301 aatagaagaa tgtcaaaaat tcactagctt agtgcgagat aaaaatcctt ttcaccaagg
     6361 ggaagcagct attgtgcctg cggctctatt attggaaaaa ataatggaag ttagaaatgg
     6421 aaaaataaag gagattcatt tgactttttt atctccagta agggtgaatc aagaattctc
     6481 agtgcaagta gtggataaca cagtgattat ttttcagcat caaatattat gtgtgagggg
     6541 gacatatgaa gaatagagat gtttatattg gttttgggct acgaacacct atcggtataa
     6601 aagggaagca atttaaacat taccgtccag aacttttagg agcacacctc ttaaatcaaa
     6661 taaaaaaaat agaatcagaa tctaatattg atagtattat ttgtgggaac acagttggta
     6721 ctgggggcaa tattggtcgt ttgatgactc ttttttctga ttatgaatcc tatattccag
     6781 tacaaacgat tgatatgcag tgtgcttcat caagttctgc cttgtttttt ggttatctaa
     6841 aaatcagtgc cggtattaat gaaaaagttc ttgttggggg gattgaaagt agttctcttc
     6901 aacctatgag acgttacgct aaagaagata atcgtaacgg agaatatacc gttgctcagt
     6961 tttctcctga ctcttatgct gaaactgtaa tgttagaagg ggcacaaaga gtctgtcaaa
     7021 aatatggttt tagaagagaa atgttagata aattggcatt cttgagccat aaacgcgcct
     7081 taacagctaa acaaggtggc tatttagaag aggtaatctt accaatggaa gggatgcgag
     7141 atcaaggcgt tagaaaacta aaagaagcat tttttcaaaa attaccaaga ttgatggaaa
     7201 attcaccttt gctcactatt ggaaatgttt gtttaatgca cgatgctgct gcatttctaa
     7261 cgcttcagag tcagaaaaca gaattccgga ttgttcacat tgtagaagta gcaggagatc
     7321 ccaagcttag tccagaattg gttcacacgg ctacggaaaa actattaaca gaaactcata
     7381 ctaaaatatc ggattatgat gcaattgaat ggaatgaacc atttgcagcg attgatgctt
     7441 tatttaatca ttattatcct gaagagagag aaaaattcaa tatttttgga ggggcattag
     7501 cttacggaca cccttatgcc tgctcaggaa ttattaatat ccttcatctt atgcaggcat
     7561 taaaatataa aaataaacct atgggtctaa ctgccattgc aggggcagga ggagtcggaa
     7621 tggccatatc aatagagtac ttaggagtta agaatgcttg aatcattgaa aacaatagtt
     7681 aaaaccaata gcgataaaaa gttatttgat ggtgatttac aggtcagtta tggagaattt
     7741 tataacttag tcagacaaga tatggcgtca caagacaata ggaaacatgt gattagcaca
     7801 cactctctgc ttaaccaatt agtaagattt gtttcaaagt tatgtcaaaa agctcttcct
     7861 attatttgta aacctaacct tactcataat gaaataagtc gattagaaaa agaagtgcag
     7921 tatgctcctc aactagctga ttttggtgtt ctttcttctg ggactacagc tgatgctaaa
     7981 ctattgtgga ggagcttcgc ttcgtggtct gattttttta gcattcagaa tgcttatttt
     8041 tcggtcactt ccaatagcag actgtttatt caaggtgatt tttcatttac tggtaactta
     8101 aatttagcgc tatccctttt attactgggg ggaacgcttg ttgtcacaca gaaaaattct
     8161 gtaaaatact ggcagacttt atgggaaaaa actggtgtaa cgcatctata tctgctccct
     8221 agttatctca agttagtgga acaatatagc aaggagactg cgttagacaa taaaactatt
     8281 attacctctt cgcaatatgt ctctgactca ttactggaag gtttatatat aaagcatcct
     8341 aaagtgtcag tgaaaatctt ctatggtgct agtgaactca attatgtgtc atggtacgat
     8401 ggtcgtgata tccgagataa gccacagtat gttggagaaa tcgttccaaa tgtagctgtt
     8461 aggattaaag agggaagaat ttttgtaaaa acaccgtata gtatctgtgg cctttcaagt
     8521 gaatattgtg ctggtgatta tggtgaactg atagatggca aattatatct atttggtcgt
     8581 ggtgacgatt ggtgcaatca atcgggcatt aagctctatt tacctagatt aattgaaaaa
     8641 ataaaaacat gcccttatat caaggatgca gtagcattta caaaagagag tcaaagtcat
     8701 ggacaagagt cacattgctg tattgtttta attgaaaatc agatgcaaca agagtgtttg
     8761 aagtggttat cggaacattt tgaaaagaag tatgggttta agcattatca tattgtatct
     8821 aaaattcctt tgatgccatc tggaaaaatt gattatcaac aactcaaaag acaattagca
     8881 tgagttttta ctaaaaaaat aaaatagggt taatggattg caaacctcag ttctgatatt
     8941 ctttattttg aaagatatcg tgatataatg ataaaaaaga aagataagga ataaaatgtt
     9001 atcaaaagca aaatcacgat atattatccg tgagattatt aagttatttc cagatgctaa
     9061 acctagctta gactttacta atgtctttga gcttttggta gctgtaatgc tatcagctca
     9121 aacaactgat gcagctgtta ataaagtcac tcccgccctt tttgaacgct ttccaaatcc
     9181 tttagttttg gcacaagctg atcccaaaga gattgaaccc tatatttcta aaattgggct
     9241 ttaccgtaat aaagctcgat tcttaaatca atgcgctaaa caattgatag agcattttga
     9301 tgggaaagtc ccccaaactc gtcaagagct agaaagtttg tctggcgttg gaaggaaaac
     9361 ggctaatgtg gtcatgagtg ttggatttgg tattccggct tttgccgtag atactcatgt
     9421 cactcggatt tgtaaacacc accagatttg caaacagtcg gcttctccac tagaaattga
     9481 gaaacgagtc atggaggttt taccgccaga agagtggcta gctgctcatc aatccatgat
     9541 ttattttggt cgtgcaattt gtcatcctaa aaatccaaaa tgcgaccagt acccacaact
     9601 ttatcacttt cctgataatt taaagtgatt agaataaaag agagtaactg gtgacgatta
     9661 tataagcgat acttaaaaac ggtagaaaag ctatcgtttt tttcattttt ctcataacta
     9721 aacaataaat aattcctaac caagcaccta tttgaataat aaaaagcatt tgatgaagag
     9781 gtaaagataa gccaattgtg gctaaataaa ggaaatctcc acttccgata ttaatatcaa
     9841 ccacttcagc taaaatccca aataaacata aaacaataga aacttttgta atcggtgtga
     9901 gaattatcgt t
//
LOCUS       DATROD010000064         9896 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.064, whole genome shotgun sequence.
ACCESSION   DATROD010000064 DATROD010000000
VERSION     DATROD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9896
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.064"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            30..503
                     /locus_tag="VBC74_001396"
     CDS             30..503
                     /locus_tag="VBC74_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEO6942339.1"
                     /translation="MSKKNKIKKTLVDQILDKAKIEHDSLQLDALQGDLPNGIQKQDI
                     FKTLALIGDKTGPIIGILPLTEHLSEKKLAKISGNKKVQMIPQKDLQKITGYIHGANN
                     PIGIRQKHNYPIFIDTIALEKQELIVSAGEIGRSIRINSEVLADFVNAKFADIKE"
     gene            525..1166
                     /locus_tag="VBC74_001397"
     CDS             525..1166
                     /locus_tag="VBC74_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEO6942340.1"
                     /translation="MKFYLVRHGKTQWNLEGRFQGANGDSPLLEEAIEELEELGQYLS
                     SIHFDAVYSSDLGRARDTVNILNDANSCPKEIHYTSQLREWALGTLEGCKIATMQAIY
                     PRQMTAFYQNPLQFKHDMFGAESLYQTTHRVESFLRSLASKNYDKVLIVGHGANLTAS
                     IRSLLGYQYGSLHYKDKLDNASLTIIETHDFKDFNCLTWNDKSYLRQEVKMTH"
     gene            complement(1202..2104)
                     /locus_tag="VBC74_001398"
     CDS             complement(1202..2104)
                     /locus_tag="VBC74_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEO6942341.1"
                     /translation="MITSIVFDVDDTIYDQQAPYRIAVEKCFPDFDMSAINQAYIRFR
                     HYSDIGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEATGIYFQEIYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVKKLGLYDYVDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPSTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRSLKPGIKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDINDKSNPVLEMGLNNGLMMAAERLLESNMS
                     VDKVVILLRLTAKQEKVLRMKYAR"
     gene            2279..3769
                     /gene="lysS"
                     /locus_tag="VBC74_001399"
     CDS             2279..3769
                     /gene="lysS"
                     /locus_tag="VBC74_001399"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEO6942342.1"
                     /translation="MSNQHIEELNDQQIVRREKMAALTEQGIDPFGKRFERTATSGQL
                     NEKYADKSKEDLHDIEETATIAGRLMTKRGKGKVGFAHIQDREGQIQIYVRKDSVGEE
                     NYEIFKKADLGDFLGVEGQVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDIETI
                     YRKRHLDLISNRDSFDRFVTRSKIISEIRRFMDSNGFLEVETPVLHNEAGGASARPFI
                     THHNAQDIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEAYQ
                     AYADYQDIMDLTEGIIQHVTKTVKGDGPINYQGTEIKINEPFKRVHMVDAVKEITDID
                     FWKEMTLEEAQALAQEKNVPLEKHFTTVGHIINAFFEEFVEDTLIQPTFVFGHPVEVS
                     PLAKKNDTDPRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQASAKELGDDEATG
                     VDYDYVEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3844..4314)
                     /locus_tag="VBC74_001400"
     CDS             complement(3844..4314)
                     /locus_tag="VBC74_001400"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEO6942343.1"
                     /translation="MTIIEGQLVANEMKIGIVVSRFNELITSKLLSGAVDGLLRHGVS
                     EEDIDIVWVPGAFEIPYMARKMALYKDYDAIICLGVVIKGSTDHYDYVCNEVTKGIGH
                     LNSQSDIPHIFGVLTTDNIEQAIERAGTKAGNKGYDCALSAIEMVNLDKKLRER"
     gene            complement(4329..5522)
                     /locus_tag="VBC74_001401"
     CDS             complement(4329..5522)
                     /locus_tag="VBC74_001401"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEO6942344.1"
                     /translation="MAFSPIKKLLQDIKSGEMVVLMDDENRENEGDLICAAEMVTKES
                     INFMAKFGKGLICLPLSNYYAEKLELAQMASHNTDNHETAFTISIDHLSTSTGISAED
                     RALTAKMVANDSSKAKDFRRPGHLFPLLAKEGGVLARNGHTEATVDLCRLAGLKECGL
                     CCEIMAEDGSMMRKDELLAFAQKHDLAIATIKQLQDYRRQEEGGVVREIEIQLPTQFG
                     HFKAYGYSEVVANKEHVALVKGDISSGEDVLCRLHSECLTGDVFHSLRCDCGEQLANA
                     LQQIEAEGRGVLLYMRQEGRGIGLINKLKAYHLQEEGLDTLEANLALGFEGDERDYGV
                     SAQLLKDLGINSINLLTNNPDKIQQLEAEGICVKNRVPLQVAATAYDLNYLKTKKEKM
                     GHLLD"
     gene            complement(5540..6190)
                     /locus_tag="VBC74_001402"
     CDS             complement(5540..6190)
                     /locus_tag="VBC74_001402"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEO6942345.1"
                     /translation="MFTGIIEEMGQVSRIRNGIKSQQLSIDVPKLVPLLRKGDSVAVN
                     GVCLTVLDKSEKAFIADVMPESMMRTSLAALRLHSKVNLELALRSDSRLGGHFVLGHV
                     DGVGKIEKIQKDDIAVRFSIDAPPSIMSYIIEKGSVALDGISLTVVSFTEHSFEVSVV
                     PHTMAQTNLSLKKVGDLLNIEVDVLGKYAEKFLAPTNRTNHTSSVMDWSFLSENGY"
     gene            complement(6171..7280)
                     /gene="ribD"
                     /locus_tag="VBC74_001403"
     CDS             complement(6171..7280)
                     /gene="ribD"
                     /locus_tag="VBC74_001403"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEO6942346.1"
                     /translation="MMKKEDYMALALKEAEKGMGFVAPNPLVGAVIVKDDRIISKGYH
                     KRFGDLHAERQAIKNADEDISGSTLYVTLEPCCHVGKQPPCTEALIKSGIKKVVVGSL
                     DPNPLVSGKGIALLRKEGLNVEVGILREECDALNERFIFHMTYKQPFVYLKYAMTLDG
                     KIATKTGDSKWISNEHSRQSVQKLRQKCSAIMVGINTVLADNPRLTCRIPKGEALVRI
                     VCDSQLKIPLDSYLVKSAKTIPTWIATCSDNLAQQQTLKEMGCRLIKVPRKDGKLDLK
                     VLMTILGQEGIDSLLIEGGSSLHFSALEAGIVNRLIVFIAPKIIGGLKAKTAISGEGL
                     DWLNQAFRVKDIELSRMDSDVVIEGKVEHYVYRNY"
     regulatory      complement(7474..7588)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            8008..9348
                     /locus_tag="VBC74_001404"
     CDS             8008..9348
                     /locus_tag="VBC74_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001073016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEO6942347.1"
                     /translation="MNQNHSVKRSLSEVNQSVEVPHNSSFWNTLRAFLGPGALVAVGY
                     MDPGNWITSVIGGATYRYLLLFVVLVSSLMAMQLQQMAGKLGIVTRQDLAQATASRLP
                     KPLRYLLFIIIELALIATDLAEVIGSAIALHLLFGWPLLLSIMITILDVFLLLLLMKL
                     GVQKIEAFVSVLILTILIIFTYLVVLSQPDLDAMFKGFLPHHELFNISHEGKNSPLTL
                     ALGIIGATVMPHNLYLHSSLSQTRRVDYHNKSSIKKAVRFMTLDSNIQLSLAFVVNSL
                     LLVLGASLFYGHANDISAFSQMYLALSDKTITGAVASSFLSTLFAVALLASGQNSTIT
                     GTLTGQIVMEGFLHFKLPQWLIRLCTRLLTLLPIFAIALLVGGEENTLDQLIVYSQVF
                     LSLALPFSIFPLIYFTSQKSIMGEHANTKWNTYLAYLIAIILTLLNLKLIMDLL"
     gene            complement(9450..>9896)
                     /locus_tag="VBC74_001405"
     CDS             complement(9450..>9896)
                     /locus_tag="VBC74_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942348.1"
                     /translation="YCILLVIIIKILSKNKSKLLLRITFGIHAGWLLVASLVNLAVYL
                     VKIDFNYPLPKVYIAIIALIFITVLSLYLARVLQNAYLILSVFWAWLMVFKAHLEGNF
                     QHLYFSFQILSLIAAIILGVSFFIIGYYNVKERYKQKRTSPYSRVL"
BASE COUNT         3166 a         1981 c         1522 g         3227 t
ORIGIN      
        1 tgaaacaaac ttattagtta agggtcatta tgagtaaaaa gaataaaatc aaaaaaactt
       61 tagttgatca aattttagat aaagcaaaaa tcgaacatga tagtctacaa ttggatgctt
      121 tacagggaga tttacccaac ggtattcaaa aacaagatat ctttaagaca ttagcgctaa
      181 tcggtgataa aactggtccc ataataggta tcttacctct caccgagcat ttatccgaaa
      241 aaaagttagc taaaatttca ggaaacaaaa aggtacaaat gatacctcaa aaagatctcc
      301 agaaaataac aggttatatt cacggagcaa ataatccaat tggaattcgc caaaaacata
      361 attaccctat ttttatcgat actatcgctt tagaaaaaca agaactcatt gtctctgcag
      421 gagaaattgg acgttctatt cgtattaata gtgaggttct tgcagatttc gtaaatgcta
      481 aatttgccga tataaaggaa taataattag gattggagaa aaatatgaaa ttttatcttg
      541 ttagacacgg aaaaacacaa tggaatttag aagggcgttt ccaaggtgct aacggagact
      601 cacctctcct tgaagaagct attgaagaat tagaagaact tggacaatac ctatcatcta
      661 ttcattttga tgctgtttat tctagcgact taggtcgtgc tagagatact gttaatatcc
      721 tcaatgatgc taattcatgc ccaaaagaaa tccactatac ttctcagctt cgtgaatggg
      781 cattgggaac tttagagggt tgtaaaattg caactatgca agctatctat cctcgtcaaa
      841 tgaccgcatt ttatcaaaat cccttacaat ttaaacatga catgttcggt gcagaatctc
      901 tttatcagac aacgcatcgt gtagagagtt tcttacgttc tttagcttca aaaaattacg
      961 acaaggtcct tattgtagga catggagcaa atctaactgc gtctattcgt tccttactgg
     1021 gttatcaata tggtagttta cactacaaag ataaacttga taatgccagc ctcacaatca
     1081 tagaaactca tgattttaaa gacttcaact gtctgacttg gaacgataaa tcttatttaa
     1141 ggcaagaggt caaaatgaca cactaaaata atccactaga ttcatctagt ggattattta
     1201 attatctagc atacttcatg cgtaatactt tttcctgttt tgcagttagg cgcagtaaaa
     1261 taacaacttt gtcaacgctc atattactct caagcagacg ctctgctgcc atcattaaac
     1321 cattattaag tcccatttca aggactggat tacttttatc gttgatatca aaaataaatt
     1381 tattatcagg taagtcaaac aacactttaa cagcaccgaa taattgctca aagttatcaa
     1441 tagcaacatc ataaactggt ttaattcccg gttttaaaga acgtcctcta tggttgaacc
     1501 acatagaatg ccaaccacca ttaaatgcac ccataatatc attatcatat gaatcaccca
     1561 cataaagtgt agttgaaggg ttcatatcaa attgctctgc tgctaaatta aaaatctcct
     1621 tttcaggctt ttgaaaacca gttgcttgac taactataac acgttttgga tcaacatagt
     1681 cataaagtcc caatttttta acttttttca attgatgttc cgtagggcca tttgtaataa
     1741 ttcccattgg tacatttttt gatttcaaaa agtcaagtgt catacgcatc tcatcaagca
     1801 ttgtaatatt ttcaagttca tgttcgtata tttcttgaaa ataaatacca gtagcttcat
     1861 caatttcacg ataaccaaac tctaaaagcg tttctttgca tcgccagaaa cgaaaatatt
     1921 ctgttgtcca ttcaccagcc attactcgtg gaaagccaat atctgaataa tgacgaaagc
     1981 gaatataagc ttggttaata gcgctcatat caaaatcagg aaagcatttc tccacagcaa
     2041 tacggtaagg tgcttgttga tcgtaaattg tatcatcaac atcaaaaaca attgaagtaa
     2101 tcatgattat ccttttcttt ctgaattttc aattgttaat tatacctttt tctaccctta
     2161 ttttcaaatg gtgaaaagtc tattaaaaat gagaataaca ccataaactc ataaaataaa
     2221 ttatgaatta tcagtgtttt tgtggtacaa taggatgaat actaacttgg aggaaattat
     2281 gtctaaccaa catattgaag aattaaatga ccaacagatt gttcgtcgtg aaaaaatggc
     2341 tgctttgact gaacaaggca ttgatccttt tggtaaacga tttgaacgta cagcaacatc
     2401 tggtcaatta aatgaaaaat acgctgacaa atcaaaagaa gacttgcacg acattgaaga
     2461 aactgctaca atcgctggtc gtttaatgac taagcgcggt aaggggaaag ttggctttgc
     2521 gcatatccaa gatcgcgaag gtcaaattca aatttatgtc cgtaaggatt ctgttggcga
     2581 agaaaactac gaaatcttta aaaaagctga tttaggtgac ttccttggtg ttgaagggca
     2641 agtaatgcgt acagatatgg gagaactttc tatcaaggct actcatatta ctcacctttc
     2701 aaaagcactc cgtccacttc ctgaaaaatt ccacggccta actgatattg aaacaatcta
     2761 tcgtaaacgt catcttgatt taatctcaaa tcgtgacagc tttgaccgtt ttgtaacacg
     2821 ctcaaaaatc atttcagaaa ttcgtcgctt tatggatagc aatggtttct tagaggttga
     2881 aacacctgtt cttcataatg aagctggtgg tgcttctgct cgcccattta tcacacatca
     2941 caatgcacaa gacattgaca tggttcttcg tatcgcaact gaattgcatc taaaacgtct
     3001 tattgtaggt ggtatggagc gtgtttatga aatcggacgt attttccgta atgaagggat
     3061 ggatgcaacc cacaatccag agttcacatc tattgaagcc taccaagcct atgctgacta
     3121 ccaggatatt atggacttga cagaaggtat tatccagcat gttacaaaaa ctgtaaaagg
     3181 tgatggacca atcaattatc aaggcacaga aattaaaatt aatgaaccat tcaaacgtgt
     3241 tcatatggtt gatgcggtta aagaaatcac tgatattgac ttctggaaag aaatgactct
     3301 tgaagaagca caagctttgg cacaagaaaa aaatgttcct cttgaaaaac atttcacaac
     3361 agttggtcac atcattaacg ccttcttcga agaattcgtt gaagacactt taattcaacc
     3421 aacttttgtc tttggtcatc cagtcgaagt gtctccactt gctaagaaaa atgatacaga
     3481 ccctcgtttt acagaccgtt tcgaactctt tatcatgact aaagaatatg ctaatgcttt
     3541 tacagaatta aatgacccaa ttgatcagct atctcgcttt gaagcacaag catctgctaa
     3601 agaacttggc gatgatgaag caactggagt tgattacgat tatgttgaag cacttgaata
     3661 tggtatgcca cctactggtg gactaggtat tggtatcgac cgtctctgta tgctgttaac
     3721 tgacacaaca actatccgag acgtcctact tttccctaca atgaaataag aatatattaa
     3781 aacccaccct tatttaaggt gggttttaat attacctcta ctgaactttg ttaggtttct
     3841 gatttatcgc tcacgcaatt ttttatcaag gtttaccatt tcaatagccg aaagagcaca
     3901 atcataacct ttatttcctg cttttgttcc tgccctctca atagcttgtt cgatattatc
     3961 cgttgttaga acaccaaaga tatgagggat atctgactgg ctatttaaat gaccaatacc
     4021 ttttgtcacc tcattacaaa cataatcata atgatcagtg ctgcctttaa tgactacccc
     4081 aagacagata atagcatcat aatctttata taatgccatt tttctagcca tataggggat
     4141 ttcaaaagca ccgggtaccc atacaatatc tatatcttct tctgaaacac catgtctcaa
     4201 cagtccatct actgctcctg ataataattt agatgtaatc aattcattga aacgtgagac
     4261 tacgattcct attttcattt cgtttgccac taattgtcct tcaataattg tcattttttc
     4321 ttcctccttt aatctaaaag atgtcccatt ttttcttttt tagtttttaa ataattaagg
     4381 tcataagctg ttgctgccac ctgtaatggc actctgtttt tgacgcaaat tccttctgct
     4441 tcaagttgtt ggattttatc tggattattg gttagtaagt ttatagaatt tatacctaaa
     4501 tcttttaaaa gttgagcact aactccatag tctctctcat ctccttcaaa acctaaagcc
     4561 aaattagctt ctaaagtatc taagccttct tcttgcaaat gataagcttt taacttatta
     4621 atcaaaccaa ttcctcgacc ttcctgtctc atataaagaa ggacgccacg accttcagcc
     4681 tctatttgtt ggagtgcatt agccagttgt tccccacaat cacatctcaa ggaatgaaag
     4741 acatcacctg tcaaacattc tgagtgtaaa cgacataaaa catcttcacc actactaata
     4801 tcccctttaa caagggcaac atgttcctta tttgccacaa cttcactata tccataagcc
     4861 ttaaaatggc cgaattgcgt tggtagctga atctcaattt ctctaaccac ccctccctct
     4921 tcttgacgtc tatagtcttg caattgtttt atggtagcga tagctaaatc gtgcttttga
     4981 gcaaatgcta ataactcatc ctttctcatc atactaccat cttcagccat tatttcacaa
     5041 caaagtccac attcctttaa tccagctaaa cgacatagat caacggttgc ttcagtatgt
     5101 ccatttctag ctaaaacacc tccttcctta gctaacaagg gaaataagtg cccaggacgt
     5161 ctaaagtctt tagctttgct actgtcgtta gcaaccatct tagctgtcaa agctctatct
     5221 tccgcagaaa tcccagtact agttgataga tgatcaatgg atatggtaaa agctgtttca
     5281 tgattatcag tattatggct agccatttga gctaattcta acttttcagc atagtaatta
     5341 gacagaggta agcaaattaa tcctttccca aatttagcca taaaattaat cgactctttt
     5401 gtcaccattt cagccgcaca aataaggtcc ccttcattct ctctattttc gtcatccatc
     5461 aaaactacca tttcccctga tttgatgtct tgtaaaagtt tttttattgg actaaaggcc
     5521 attcgctttc tcctctctct tagtagccat tttctgataa aaaagaccaa tccattactg
     5581 atgaagtgtg attagttcta ttagtcggtg ctaaaaattt ctcagcatat ttgcctaaaa
     5641 catccacttc gatatttaat aaatccccaa ccttttttaa ggataaatta gtttgagcca
     5701 tcgtgtgggg aacgaccgaa acttcaaaag aatgctctgt aaatgatacc accgttaggc
     5761 taatcccatc tagagccacc gaacctttct ctataatata agacataata ctgggcggag
     5821 catcaattga aaatctgaca gcaatatcat ccttttggat tttttctatt tttccaaccc
     5881 catccacatg tccaagaaca aaatgccccc ctaaccttga gtcacttctg agtgctaatt
     5941 ctaagttaac tttagaatga agtcgtaaag cagctaagct tgttcgcatc atactttctg
     6001 gcatgacgtc tgctataaaa gctttttcac ttttgtcaag gactgtcaaa caaacaccat
     6061 taacagctac gctatcccct tttcttaata aagggactag tttgggaaca tcaatactta
     6121 attgttggga ttttatgcca ttccgaatac gactgacttg acccatttcc tcaataattc
     6181 ctgtaaacat aatgttctac ctttccttca ataacaacgt cagaatccat acgactaagt
     6241 tcaatatctt ttactctgaa agcttggtta agccaatcca agccttctcc agaaatagct
     6301 gtttttgctt tcagacctcc tattattttg ggtgcaataa aaacaattag tctgttaaca
     6361 ataccagctt ctaaagctga aaaatgtaaa ctacttcctc cttcaataag aaggctatca
     6421 atcccttctt gtcctaaaat agtcattaat actttcaaat ccagctttcc atcttttctt
     6481 gggaccttaa tcaaacggca acccatttct tttaatgttt gttgctgagc aagattatct
     6541 gaacatgttg caatccatgt tggtattgtt ttagcgctct taactaggta agagtctaaa
     6601 ggtatcttca actgactatc acatacgatc ctcacaagcg cttcaccttt aggaatacgg
     6661 caagttaaac gtggattatc agctaataca gtattgatac cgaccataat agcactacat
     6721 ttttgcctca atttctgaac agattgtctt gagtgctcat tggatatcca cttagaatcg
     6781 cccgtctttg tagctatctt cccatctaaa gtcatagcat atttcaaata aacaaaaggc
     6841 tgcttatagg tcatgtgaaa aataaatctt tcattcagag catcgcattc ttctcttagg
     6901 ataccaactt caacatttaa accttctttt cttaacaagg caatcccttt tcctgaaact
     6961 aaaggatttg gatctaaact tccaactact acttttttga taccagattt aataagagct
     7021 tcagtacaag gtggttgctt acccacatga cagcatggtt ctaaagtcac gtacagagta
     7081 cttccagaga tatcctcatc agcattttta atagcctgtc tttccgcatg taaatctcca
     7141 aatcgtttat gataaccttt ggaaattata cgatcatctt taacaataac agcacctact
     7201 aatgggttgg gagcaacgaa ccccatacct ttctcagcct ctttcaaggc taatgccatg
     7261 tagtcttctt ttttcatcat ttcctttttc cttctaaaat aaaaaacctt agcttctaaa
     7321 atagaaaact aaggggaggg atgagaagtc gttgattata ctaattagca ttatatttta
     7381 ggtagcacaa aaaaactaaa gactccctat agaaaactat agggagtctc tcattaatcc
     7441 agcacaaata aactgcaagt agcagacaat cactgaattc ttctctcatc cagactttac
     7501 tgtcggtact ggaatcacac cagttcatgc tcgaaagctc gcggactata ccgccggtta
     7561 ggattttcac ctgaccccga agaaattcta tttgacagtt attttactaa actcattttt
     7621 atttgtcaat aaaaataatg tgttttttat tataaaataa taattaagta gctcatctat
     7681 tcttatcttt aaaagacagt atatctatac cataaagcaa tctttcgaac taatcaaaca
     7741 agaatcgata gtattattgg attatatttc ttaaaaaatt cactacagat atagtttact
     7801 ccacttaaat aacttaggta aatctacgga actaaaaata atcgatagaa atctgttatt
     7861 ttgttatttc aaatgtaact gttaagaaaa tagttctcta atactaaaca gcacctcaat
     7921 cgattattaa gtagcattaa tttttaattg tttttctatt ttctatttgt tataatggct
     7981 tgagattact agaaagtata gtattacatg aatcaaaatc attccgtcaa aagatcctta
     8041 tccgaagtta atcagagcgt tgaggttcct cacaatagct ctttttggaa cactttaaga
     8101 gcttttcttg gacctggagc tttggttgca gttggttata tggatccagg taactggatt
     8161 actagcgtta ttggtggagc tacctatcgt tacttactat tgtttgttgt tttagtatct
     8221 tctttgatgg cgatgcaatt acaacaaatg gctggcaagc ttggtattgt tacacgacag
     8281 gacttagcac aagcaacagc ttctcgactc ccaaaaccgc ttcgttacct gctatttatt
     8341 atcattgagt tagcccttat cgctactgat ttagcagaag tcattggctc tgccattgct
     8401 cttcacttat tatttggctg gcccctgtta ctgtctatca tgattactat tttggatgta
     8461 ttcttacttt tactcttaat gaagttaggc gtccaaaaaa ttgaagcctt tgtttctgtt
     8521 ctcatattga caattttgat aatctttaca tatttagttg ttttatcaca acctgattta
     8581 gatgctatgt tcaaaggatt tttaccacac catgagcttt ttaacatatc acatgaaggg
     8641 aaaaatagtc ctttaaccct cgctcttggc attatcggcg caacagtcat gccacataat
     8701 ctttatttac attcttcttt atctcagact agacgcgtgg attatcataa taaatcttca
     8761 ataaaaaaag ctgtacgttt catgacctta gattctaaca ttcagctatc acttgcattt
     8821 gtcgtgaatt cgctactatt agttttagga gctagtctat tttatgggca tgctaatgat
     8881 atcagtgcct tttctcaaat gtatcttgcc ttatctgata aaactattac tggtgcagta
     8941 gccagttctt tcctttctac cctttttgca gttgccctac tagcaagtgg tcaaaattca
     9001 acaattacag gtactttaac aggacaaatc gttatggaag gtttccttca cttcaaatta
     9061 cctcaatggt taataaggct ttgtactaga ttattaactc ttcttccaat ttttgctatc
     9121 gccctactag ttggaggcga agaaaatacg ttagatcaac taatcgtcta ttcacaagtt
     9181 tttctttcct tagctttacc attttcaatc tttccattga tttatttcac atcgcaaaaa
     9241 tcaattatgg gagagcatgc taatactaaa tggaatacct atttagcata ccttatcgct
     9301 atcattttaa cattactcaa tttgaaatta atcatggatt tactttaata caatacaaaa
     9361 aagccccact aatgaactga acctctatca tgagacatac atctaatgat agaggaaagt
     9421 acataatgat ggagcttttt gtatttcttt taaagaaccc gtgagtaagg actagtacgt
     9481 ttttgcttgt acctctcctt gacattataa taaccgatga tgaaaaacga tacgccaagg
     9541 ataattgctg caatcaaaga taaaatttgg aaactaaaat ataggtgttg aaaatttcct
     9601 tctaaatgtg ctttaaaaac catcaaccaa gcccaaaaaa cggataagat taagtaagca
     9661 ttttgtaata ccctagccag gtaaagactt aaaacagtta taaaaatcaa ggcaatgata
     9721 gctatatata cctttggtaa tgggtaatta aaatcaattt taactaagta gaccgctaaa
     9781 ttgaccaatg acgcaactag caaccatcct gcatgaattc caaatgttat tctaagcagt
     9841 aattttgatt tatttttact tagtatcttg ataatgataa ccagaagaat acagta
//
LOCUS       DATROD010000065         9883 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.065, whole genome shotgun sequence.
ACCESSION   DATROD010000065 DATROD010000000
VERSION     DATROD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9883
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.065"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..782)
                     /locus_tag="VBC74_001406"
     CDS             complement(<1..782)
                     /locus_tag="VBC74_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEO6942349.1"
                     /translation="MTYTSLKSPTTKDYKYTLNVAHLYGNLLNTYGDNGNILMMKYVG
                     EKLGCQMTFDIVSLEDRFDPNYYQMAFFGGGQDYEQAIVARDLPSKKEDINKFIQNNG
                     VVLAICGGFQLLGQYYIQANGERIEGIGVMGHYTLNQNNNRYIGDIKIHNDEFNETYY
                     GFENHQGRTFLSEDEKPLGTVIYGNGNNKEDGTEGVHYKNVFGSYFHGPILSRNANLA
                     YRLVATALRNKYGKEIVLPSYEEILSLEIPEEYGDVKSKADF"
     gene            complement(782..2125)
                     /locus_tag="VBC74_001407"
     CDS             complement(782..2125)
                     /locus_tag="VBC74_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEO6942350.1"
                     /translation="MKINTALGVAAGKSAHYLLSKMGRGSTYPGSLALKFDKDILDTI
                     AKDYEIVVVTGTNGKTLTTALTVGILKEAFGQVVTNPSGANMITGIVSTFLTAKKSKS
                     GKKIAVLEIDEASLPRITQYIKPSLFVFTNIFRDQMDRYGEIYTTYQMILDGAANAPQ
                     ATILANGDSPLFNSKSVTNPVQFYGFDTDKHEPRLAHYNTEGILCPKCQAILTYRLNT
                     YANLGDYTCPNCDFERPNLDYALTRLIHLTNTSSGFVIDGQQYNINVGGLYNIYNALA
                     AVSVAEYFGVEASQIKDGFDKSRAVFGRQETFTIGNKKCTLVLIKNPVGASQALDMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDANFETILTMNIPEIFAGGVRHSEIARRLRVT
                     GYDEKRIKQADKLQDIMAMIEQQETEHAYILATYTAMLEFREILANHNAIRKEMN"
     gene            2265..3116
                     /locus_tag="VBC74_001408"
     CDS             2265..3116
                     /locus_tag="VBC74_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEO6942351.1"
                     /translation="MDIFSAIDSKFWASIMENPWMILIHLMDIIIVAVLIYKFIKALA
                     GTKIMSLIQGVILFIIIRFVSEWIGLTTITFLMNQIVTYGVIAGVVIFAPEIRTGLEK
                     FGRTPQLFTQRSQLSSDEKLVDALVKAVAYMSPRKIGALISIERTQTLQEYIATGIPL
                     DADISSELLINIFIPNTPLHDGAVIVKDKKIATACSYLPLSESSSISKEFGTRHRAAI
                     GLSENSDALTVIVSEETGGISVALKGEFLHDLSKDSFEAILRTQLIQNQEENSKLAWY
                     NQLLRRK"
     gene            3119..4078
                     /locus_tag="VBC74_001409"
     CDS             3119..4078
                     /locus_tag="VBC74_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEO6942352.1"
                     /translation="MKKFFTNKFWLGVVSLFLAILLFLTATATSMNHQDNSKIAGASE
                     TYTHTLTDVPIDIKYDSDDYFISGYSYGADVYMSSVNRVKLDSEINEDTRKFKVVADL
                     TNMKPGTHKVPLKVVNLPSGVNATVSPTTITVTMGKKKTKEFPVYGHVNDKQIKAGYA
                     VDKMSVDVSKVKVTSDESIIDRIDHVAANIPDDKVLDDDFNKTVTLQAVTADGTVLAS
                     IIHPSKATLSVKVKKLTKTVPINLIPVGQFSDSISKINYKLSQEKAVISGTKEALEAI
                     SVINAEVDISDVTKNTEKKINLSANNVSVDPAQVTVQLTTTKK"
     gene            4132..5484
                     /locus_tag="VBC74_001410"
     CDS             4132..5484
                     /locus_tag="VBC74_001410"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEO6942353.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETDRPRV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGSDGFKLDDDRELEIEALLDAKEDTLPRPSAQGLGTLVDYPEGLRKY
                     EKFMESTGIDLEGMNVALDTANGAATASARNIFLDLNADISVIGDQPDGLNINDGVGS
                     THPEQLQSLVRENGSDIGLAFDGDSDRLIAVDENGEIVDGDKIMFIIGKYLSDKGQLA
                     QNTIVTTVMSNLGFHKALDREGIHKAITAVGDRYVVEEMRKSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLTKVMKETGKKLSELASEVTVYPQKLVNIRVENNMKDKAMEVPAI
                     AEIIAKMEEEMDGNGRILVRPSGTEPLLRVMAEAPTNEAVDYYVDTIADVVRTEIGLD
                     "
     gene            5607..5978
                     /locus_tag="VBC74_001411"
     CDS             5607..5978
                     /locus_tag="VBC74_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEO6942354.1"
                     /translation="MTKEAELLWAKHKYLVLSKSQKIYLDIRQTLKSPNCTVLDVQSL
                     IDQAVLLEESPSQVTNAYMHIWGYFKNKAERQEKEEFLTLLEKYRKTGYQRRKLLAFL
                     KQLLAKYPNSYLQNSSIFEEE"
     gene            6003..6383
                     /locus_tag="VBC74_001412"
     CDS             6003..6383
                     /locus_tag="VBC74_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942355.1"
                     /translation="MRLWHQDIIELLPRQQLLGQHRECCALRGNGWGRKHETVDYVFR
                     YSPYRLFAYHQLVMEEMMERGYRVSKEWLIAEYRGMKCPRYDTLNPVDLETPIYPEHN
                     QDYLQECLWNLKAKGIDLPINKIK"
     gene            6476..7606
                     /locus_tag="VBC74_001413"
     CDS             6476..7606
                     /locus_tag="VBC74_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEO6942356.1"
                     /translation="MLKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDAYLQALIRE
                     FRSYDITELRTLYIGGGTPTSISAVQLDYLLTELSRDLNLNTLEEFTIEANPGDLTVD
                     KIEVLQKSAVNRVSLGVQTFNDKHLKRIGRSHNEVQIYSTIDALKTAGFQNISIDLIY
                     ALPGQTMDDVRSNVAKALSLNIPHLSLYSLILEHHTVFMNKMRRGKLHLPTEDLEAEM
                     FEYIISEMERNGFEHYEISNFTKPGFESRHNLMYWDNVEYYGVGAGASGYLDGIRYRN
                     RGPIQHYLKGVSEGNARLSEEVLSKNEMMEEELFLGLRKKKGVSIGKFEQKFGTSFEK
                     RYGQIVQELQSDGLLKENNGFIQMTKKGLFLGDTVAEKFIVE"
     gene            7610..8347
                     /locus_tag="VBC74_001414"
     CDS             7610..8347
                     /locus_tag="VBC74_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEO6942357.1"
                     /translation="MGLLYRETYEVPFYESDTNHYMKLPQLLALALQISAKQSLKLGI
                     GDDIVFKRYGLVWVVTDYIIDIERLPKHAEKIVIETEAKAHNKLLCYRYFYIYGEDGQ
                     KIITISSAFVLMDFKTRKIHPVLDDITSIYQSQRIKKVIRGPKYHPIGDSKVKQYHVR
                     YFDLDMNGHVNNSKYLEWMYDVLDLDFLSSHIPKKIDLKYIKEIQYGTDIKSHWYQDG
                     LVTRHDIIGGDAIHAQARIEWQEKKED"
     gene            8348..9118
                     /locus_tag="VBC74_001415"
     CDS             8348..9118
                     /locus_tag="VBC74_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEO6942358.1"
                     /translation="MAYKGYLIDLDGTIYKGKSRIPAGERFIERLQEKGIPYMLVTNN
                     TTRTPESVQEMLRGFNVETPLETIYTATMATVDYMNDMNRGKTAYVIGEEGLKKAIAD
                     AGYVEDTKNPAYVVVGLDWNVTYDKLATATLAIQNGALFIGTNPDLNIPTERGLLPGA
                     GSLNALLEAATRIKPVFIGKPNAIIMNKALEILNIPRNQAVMVGDNYLTDIMAGINND
                     IDTLLVTTGFTTVEEVPDLPIQPSYVLASLDEWTFNEG"
     gene            9108..9764
                     /locus_tag="VBC74_001416"
     CDS             9108..9764
                     /locus_tag="VBC74_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEO6942359.1"
                     /translation="MKDKLLVVLTWIWIISLATLATIYIAWLIYPIEIQFLKLEKVVY
                     LKAETIYYNFNKLMIYLTHPFISDLNMPSFPSSEDGLKHFADVKYLFTLAHGLFVILT
                     FPVIYFLRRGWKQKSIFLYEGFFKIAIMLPIFIVVCAFLLGFDQFFTLFHEVLFPGDS
                     TWQFNPLTDPVIWILPETFFLHCFIIFLLIYETITIILLIIGRKHLKLRKYKNKMQAL
                     "
BASE COUNT         3245 a         1527 c         1927 g         3184 t
ORIGIN      
        1 tcaaaatctg cttttgattt aacgtctccg tattcctcag gaatctctaa agataaaatt
       61 tcttcataac tagggaggac aatctcttta ccatatttgt tacggagggc agttgccaca
      121 agacggtaag ctagattagc attacgtgaa aggattggac cgtgaaagta tgacccaaaa
      181 acattcttat aatgaacccc ttcagtacca tcttctttgt tattcccatt tccataaatg
      241 acagttccta aaggtttttc gtcttctgat aaaaaggtac gtccttgatg attctcaaag
      301 ccataatatg tttcattgaa ttcatcgttg tgaatcttga tatcaccaat atagcgatta
      361 ttattttggt ttagcgtata gtgtcccata acgccaatgc cctctattcg ttctccatta
      421 gcttgaatat agtattgtcc taaaagttgg aaacctccac aaatagcaag tactacacca
      481 ttattttgaa tgaatttgtt aatgtcttct ttcttactgg gaagatcgcg tgcaacaata
      541 gcttgttcgt agtcttgtcc accaccaaaa aatgccatct gataatagtt gggatcaaat
      601 ctatcttcta atgaaacgat atcaaatgtc atttgacatc ctagtttttc tcccacatat
      661 ttcatcatta agatattccc attatcgcca taagtattta ataggttacc ataaagatga
      721 gctacattaa gagtgtattt ataatctttg gtagtaggag attttagtga ggtataagtc
      781 attaattcat ctcctttctg atagcgttgt ggttagcaag aatttcacga aactccagca
      841 tagctgtata tgttgctaaa atataagcat gctcagtctc ttgctgctca atcattgcca
      901 taatatcttg cagtttatca gcttgtttta tgcgtttctc atcataacca gttacacgta
      961 agcgacgagc aatttcagag tggcgaacac caccagcaaa tatttctggg atattcatcg
     1021 tgagaatagt ttcaaaatta gcgtcccaaa tccaagaagt atcaataccg tcagcataat
     1081 tggcatttaa tagtactgaa agagagaacg ggtatggagc aagtttaatc atatcaagag
     1141 cttgactagc tcctactgga tttttaatca gaactaaggt acatttttta tttccaattg
     1201 taaaggtttc ttgacgacca aaaacggcac ggcttttatc aaatccatct ttaatttgac
     1261 ttgcttctac accaaaatat tcagcaacag atactgccgc caaagcattg taaatattat
     1321 aaaggccacc cacgttgata ttatattgtt ggccatcaat aacgaaacct gatgaagtat
     1381 ttgtcagatg tattagcctt gttaaggcat aatcaaggtt tggacgttca aaatcacaat
     1441 taggacaagt gtagtcacca aggttggcat atgtgttcaa tcgataagtt aaaattgctt
     1501 gacactttgg acagagaata ccctcagtat tatagtgtgc taatcgtggc tcatgtttgt
     1561 cggtatcaaa tccatagaat tgcacagggt tagtcacgct tttagaatta aaaagtggac
     1621 tgtcaccgtt agctaagata gtagcttgcg gagcgttagc agctccatct aaaatcattt
     1681 gataagttgt gtagatttcg ccataacggt ccatttgatc acggaaaata ttggtaaaga
     1741 cgaacaaact aggctttata tactgtgtaa tacggggtaa actcgcttcg tctatttcaa
     1801 ggacagctat ttttttacca gatttacttt tcttagctgt taggaaggtt gaaacaatac
     1861 ctgtaatcat atttgctcca cttgggttgg ttaccacctg accgaaggct tcctttaaaa
     1921 taccaactgt taaagctgtt gttaaggttt taccgttagt accagtcaca actacaattt
     1981 cataatcttt ggcaattgta tctagtatat ctttatcgaa tttgagtgct aaactacccg
     2041 gataagtaga accacgtccc attttactta aaaggtaatg agcagactta ccggcagcca
     2101 ctcctagtgc tgtatttatt ttcataaaga tattttatca taaaaagatg ctttcttgct
     2161 aaagaaaata ttatgaaaac atggtataat gtagacgatg gaaaataaga aatcaaggag
     2221 ttaagcaaga aactcttcga caggctacca gtagagagga agatatggat attttttcag
     2281 ctattgattc aaagttttgg gcgagcatta tggaaaatcc atggatgatc cttattcatc
     2341 tcatggatat tatcatagta gctgttctga tttataaatt tatcaaagcg ctagcaggca
     2401 caaaaattat gtccttaatt cagggcgtta ttttgtttat catcattaga tttgtgtcag
     2461 aatggatagg gctaacaacg attacatttt tgatgaatca gattgttact tatggtgtaa
     2521 ttgcaggtgt tgtcatcttt gctccagaga ttagaacagg tctcgaaaaa tttgggcgaa
     2581 caccacaact atttacgcaa cgttctcaat taagcagtga tgaaaagcta gtagatgccc
     2641 ttgttaaggc agttgcttat atgagtccgc gtaaaatagg agccttgatt tcaattgaac
     2701 gtacccaaac tttgcaagag tatatagcta caggtatccc attagatgca gatatttcca
     2761 gtgaattgtt aattaatatt ttcattccta atacaccact gcacgatggt gctgttattg
     2821 taaaagataa aaaaattgct acagcatgtt cgtacttacc tctatcagaa tcatcttcga
     2881 tttcaaaaga atttggaaca cgccacagag cagcgattgg tctatcagag aattcagatg
     2941 cactaacggt tattgtttct gaagagactg gtggtatttc tgttgctttg aaaggtgaat
     3001 ttttacatga tctttctaaa gatagctttg aagcaattct gagaactcag ttgattcaaa
     3061 atcaagagga aaactcaaaa ttagcttggt ataatcaact attgaggagg aagtagacat
     3121 gaaaaaattt tttacaaata aattttggtt aggcgttgtc tcactctttc tggctatttt
     3181 attattttta acggcaactg caacaagtat gaaccatcaa gacaatagta agattgcagg
     3241 agcgtctgag acatatacac ataccctaac agatgtgcca atagacatca aatacgatag
     3301 tgatgattac tttattagtg gctattctta tggggcagat gtctatatgt catcggttaa
     3361 tcgtgttaag ctagattctg aaataaatga ggatacacga aaatttaaag tagtcgctga
     3421 tttgacaaat atgaaaccag ggactcacaa agtcccacta aaggtagtaa atttgccatc
     3481 tggtgtaaat gctacggttt ctccaacaac aattacagta actatgggta aaaagaaaac
     3541 aaaagagttt ccggtttatg gacatgttaa tgataagcaa attaaagctg gttatgcagt
     3601 cgataagatg tcagttgatg tgtcaaaagt taaagttact agtgatgaat ctattataga
     3661 tcgtattgac catgtcgctg ccaatatccc tgatgataaa gttcttgatg atgattttaa
     3721 caagacagtt actttacaag ctgtaacagc agatggtaca gtattagcaa gtattattca
     3781 cccttctaaa gcaactttat ctgtcaaggt aaaaaaattg accaagacag taccgattaa
     3841 tttgatacca gtaggacaat tcagcgatag tatttcaaaa attaactaca agttgagtca
     3901 agaaaaagca gtaatttcag gaacaaaaga ggctttagaa gctatttctg ttattaatgc
     3961 tgaggtagat atttcagacg taactaaaaa tacagaaaag aaaattaatc tttctgcaaa
     4021 caatgtttca gttgatcctg ctcaagtaac agttcaattg acaactacta aaaaataata
     4081 gataataaaa acgaaaaaaa tggaggtagc gtttttcgct agaaaaaaat tatgggaaaa
     4141 tattttggta cagatggtgt tcgcggtgaa gctaatgttg aactgacgcc agaattggct
     4201 tttaaattag gacgttttgg aggatatgtt ctaagtcaac acgaaactga tcgtccgaga
     4261 gtttttgtag ctcgtgatac tcgtatttct ggtgaaatgc tggaatctgc tttgattgct
     4321 ggtctacttt cagtaggtat tgaggtttat aaacttggtg ttttagccac tcctggagtg
     4381 tcttaccttg ttcgtacaga aaaagctagt gcaggggtta tgatttcagc aagccacaat
     4441 ccagcacttg ataatggtat taagttcttt ggtagtgatg gttttaaact tgatgatgat
     4501 cgtgaattag aaattgaagc tttacttgat gcgaaggaag acactttacc acgtccatca
     4561 gcacaaggtt taggtacttt agtagattat cctgaaggac ttcggaaata tgagaagttt
     4621 atggaatcaa ctggtattga tttagaaggt atgaatgttg cgttggatac agcaaatggt
     4681 gcagcaacag cctcagctcg gaatatcttt ttagacttga atgctgatat aagtgttatt
     4741 ggtgatcaac cagatggttt gaatatcaat gatggtgttg gttcgacaca tcctgagcaa
     4801 ttacaaagtt tagttagaga aaatggttcc gacatcggtc tagcttttga tggtgatagt
     4861 gatcgtttga tcgctgttga tgaaaatgga gagattgtag atggtgataa aattatgttt
     4921 atcattggga aatatctctc tgataaaggg cagcttgctc agaatacaat cgtaacaacg
     4981 gttatgtcaa atctcggctt tcataaagcg ctcgaccgtg aaggaattca taaagcgatt
     5041 accgctgtag gagaccgcta cgtggttgaa gaaatgcgta aatcaggata taatcttggc
     5101 ggagagcaat caggccacgt tattatcatg gattacaata ctacagggga tggacaatta
     5161 acagccatcc aattgactaa ggtcatgaaa gaaactggta agaaattatc agagttagct
     5221 agtgaagtga cggtttatcc tcaaaaatta gtaaatattc gtgttgaaaa taacatgaaa
     5281 gataaagcga tggaagttcc tgcaattgcg gaaattattg ctaaaatgga agaagagatg
     5341 gatggcaatg gtcgtattct agtacgtcct agtggtactg agcctcttct tagagttatg
     5401 gcagaagcgc caacaaatga agcggttgat tattatgttg atactatcgc agatgtcgtt
     5461 cgtacagaaa ttggtttaga ttaaaacggc gttattctgc tataaagtcg tcaatgttat
     5521 aaaataataa agagagggaa ttggagtgga gcatcaagca tacgcctttc tctctctttt
     5581 tgatttgaga agggagttta gagaaaatga ctaaagaagc agaattatta tgggctaaac
     5641 acaagtattt agtcttaagt aaatctcaga aaatttattt ggatattaga caaactttga
     5701 aatcaccaaa ttgtaccgtt ttagacgttc agtcactcat tgaccaagca gtgcttttag
     5761 aagaatcacc atcacaagtg actaatgctt atatgcatat atggggctat tttaaaaata
     5821 aggcagagcg tcaagaaaaa gaagaatttt taaccttgct ggaaaaatac aggaaaacag
     5881 gttatcaacg aagaaaactg ttagcttttc taaaacagct attagctaaa tacccaaatt
     5941 cttatttaca aaactcttct attttcgaag aagagtaaaa gattgatagt tgaggtatat
     6001 aaatgagatt atggcatcaa gatattattg agttattgcc taggcagcaa cttttaggac
     6061 agcatagaga gtgttgtgca ttgagaggga atgggtgggg gagaaaacat gagactgttg
     6121 attatgtttt tcgatattca ccttataggt tatttgcata tcatcaatta gttatggaag
     6181 aaatgatgga gcgtgggtat cgtgtatcta aagaatggct aattgcagaa tataggggaa
     6241 tgaagtgccc aagatatgat acacttaatc ctgttgactt ggagacacct atctatcctg
     6301 agcacaatca agactatcta caagaatgtc tatggaattt aaaagcaaaa ggaattgacc
     6361 ttcctataaa taagataaaa tagagattta tctctatttt tattttggaa taaaaagcta
     6421 tattatcaag ttttagagga gtgttttttt aagggcaaag tggtaaaata gaaacatgtt
     6481 aaagaaacca acctcagcat atgtacacat tccattttgt acccaaatat gttattattg
     6541 tgatttttca aaagttttta ttaaaaatca gcctgtagac gcttatcttc aggctcttat
     6601 ccgtgaattt cggtcttatg atattacaga attgcgcacc ttatatattg gaggagggac
     6661 acctacatct ataagcgctg tgcaactgga ctatttattg acggagttaa gtagagattt
     6721 aaacttaaat actttagagg agttcactat cgaagctaat ccaggagatt taaccgttga
     6781 taaaatagag gtgcttcaaa agtcagctgt caatagagtt tctctaggag ttcagacctt
     6841 taatgataaa catttaaagc gtattggacg cagtcacaat gaggtacaaa tttattctac
     6901 aattgatgct cttaaaacag ctggttttca aaatatctct attgacctaa tttatgcctt
     6961 acctggacaa acgatggatg atgttagaag taatgtagcc aaagctctct ctctaaatat
     7021 tcctcatttg agtctatata gtttaatttt agaacaccat actgtattta tgaataaaat
     7081 gcgtcgtggg aaattacatt tgccaacaga agatttagaa gccgagatgt ttgaatatat
     7141 catttctgaa atggagagaa atggttttga gcattatgaa atttctaatt ttacaaagcc
     7201 gggttttgaa agtcgtcata atttgatgta ctgggataat gtagaatatt atggtgttgg
     7261 tgcaggtgct tctggttatc ttgatggtat tcgttatcgt aatagagggc caatacaaca
     7321 ttatctaaag ggtgtttcag aaggtaatgc aaggttatct gaagaagtat tatcaaaaaa
     7381 tgaaatgatg gaagaggagc ttttcctcgg tcttcgtaaa aaaaagggtg tttccatcgg
     7441 aaaatttgaa caaaaatttg gaacgtcctt tgagaaacgt tacggtcaga ttgttcaaga
     7501 actccaaagt gatgggcttt tgaaagaaaa taatggtttt atccagatga ctaaaaaagg
     7561 tctcttctta ggagatacgg ttgccgagaa atttattgtg gagtaaaaaa tgggtttatt
     7621 atatcgagaa acttacgaag tgccttttta tgaatcagac actaatcatt atatgaaatt
     7681 accgcagttg ttggctttag cgttacaaat ttcagccaaa caatctttaa aattaggaat
     7741 aggcgatgac atcgttttta aaagatacgg attggtttgg gtcgttacag attatattat
     7801 tgatattgag cgtttaccca agcatgctga aaagatagtc attgagactg aagccaaagc
     7861 tcataataaa ttattgtgtt accgctattt ttatatttat ggtgaagacg ggcaaaaaat
     7921 aataacgatt tcatctgctt ttgtcttaat ggattttaaa acacggaaga ttcatcctgt
     7981 tcttgatgat attacttcaa tttaccaatc ccaaagaatc aaaaaagtta ttcgagggcc
     8041 gaaatatcat cccattggag atagtaaagt taaacaatac catgttcgtt attttgattt
     8101 agatatgaat ggacatgtta ataatagtaa gtatctagaa tggatgtatg atgtgttgga
     8161 cttagatttt ctaagtagcc acattcctaa aaaaattgat ttaaaatata ttaaggaaat
     8221 tcaatatgga acggatatta agtctcattg gtatcaagat ggtcttgtga cacgtcatga
     8281 tattattggt ggcgatgcta ttcatgctca agctaggatt gaatggcagg aaaaaaagga
     8341 ggattaaatg gcttataaag gatatcttat tgatttagat ggtactattt acaaagggaa
     8401 aagtcgtatc ccagcaggag agcgttttat cgaacgtttg caagaaaaag gcattcctta
     8461 tatgttagta actaataata cgactcgcac accagaaagc gttcaggaaa tgcttcgagg
     8521 cttcaatgtt gaaacaccac ttgaaacaat ctataccgca actatggcta cggttgacta
     8581 tatgaatgat atgaaccgtg gaaaaacagc ttacgttatt ggtgaagaag ggcttaaaaa
     8641 agctattgca gatgcaggtt atgtggagga tacaaaaaat ccagcctatg tggttgttgg
     8701 tttagattgg aatgttactt atgataaatt ggcaactgct acgttagcaa ttcaaaatgg
     8761 tgctttattt attggaacca atccagattt gaatattcct actgaacgtg gtttattacc
     8821 cggtgctggc tccttaaatg ctttattaga agccgcaacg cgtataaaac cagtctttat
     8881 tggaaagcca aatgccatca ttatgaataa ggcacttgaa attttaaata ttcctagaaa
     8941 tcaagcagtc atggtcggtg ataattattt gacagatatc atggctggta ttaataatga
     9001 tattgatact ttgttagtaa caacgggttt tacaacagtt gaagaagtac cagatttacc
     9061 aattcaacca agttatgttt tagcaagttt agatgaatgg acatttaatg aaggataagt
     9121 tattagtagt tttaacttgg atttggatta tatcgttagc aactctagct acaatttata
     9181 ttgcttggtt gatctatcct atagaaattc agtttttaaa attagaaaag gttgtgtatt
     9241 taaaggctga aacaatttat tataatttta ataagttgat gatatatctg actcatcctt
     9301 ttatctctga tttaaatatg cctagctttc cttcttcaga ggatggttta aagcattttg
     9361 cggatgtaaa atatcttttt actctagcgc atgggctttt tgttatacta acttttccag
     9421 ttatttattt tttaagacgt ggatggaagc aaaaaagtat ctttctatat gaaggcttct
     9481 tcaagatagc tattatgttg cctattttta ttgtagtatg tgcttttctg ttgggttttg
     9541 atcagttttt cactctattt catgaggtgc tttttccagg agattctact tggcaattta
     9601 atcctctaac ggatccagta atttggatac ttcctgaaac ttttttcctc cattgtttca
     9661 tcattttttt gttaatctat gaaacaataa ctataattct tttgataata ggtagaaaac
     9721 atttaaaatt aagaaaatat aaaaacaaaa tgcaagcctt ataatagcgc ttgcattttg
     9781 tttgctattt ttttataata caaatatgag aaatgtcgga attataacaa tgacataaaa
     9841 aaagcaccga atcggtgctc actttttcaa gctaaatacg gac
//
LOCUS       DATROD010000066         9834 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.066, whole genome shotgun sequence.
ACCESSION   DATROD010000066 DATROD010000000
VERSION     DATROD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9834
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.066"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..814)
                     /locus_tag="VBC74_001417"
     CDS             complement(<1..814)
                     /locus_tag="VBC74_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEO6942360.1"
                     /translation="MNLLFSIDDMYVDHFKVMLYSLVRQTKNRKLEIYVLQKTLLKRH
                     TELIQYTQNLEVGYHPIIVGTEVFAQAPTTDRYPDTIYYRLLAHKFLPETLDRILYLD
                     ADMLCLNDFSSLYDMELGDQLYAAASHNTDGKFLDYVNKLRLKNVELESSYFNTGVLL
                     MNLPAIRKVVHQQTILDYIMQNRGRLILPDQDILNGLYANLVKPIPDEIYNYDARYSL
                     IYQLKSRNEWDLEWVINHTVFLHFAGRDKPWKKDYRGRYSGLYKFMAKEALTK"
     gene            complement(902..1618)
                     /locus_tag="VBC74_001418"
     CDS             complement(902..1618)
                     /locus_tag="VBC74_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEO6942361.1"
                     /translation="MVGLGTVINVILIIVGGFVGLFLKNFLKESLQKSLMQAMGVAVL
                     FISISGVLEKMMLVEKSHLISNHTNMMIITLALGTVLGELLSLDSYIDRFGDYLKQKT
                     GSGNDIKFVEAFVTSTCTVCIGAMAVVGSIQDGIAADHSILFAKGMLDMIIIAIMTVS
                     LGKGALFSALPVALLQGSLTIVAFFMGSLLNPSSLDYLNLVGNMLIFCVGVNLLFNLN
                     IKVINMLPAIILAILWGSFI"
     gene            complement(1700..4318)
                     /gene="alaS"
                     /locus_tag="VBC74_001419"
     CDS             complement(1700..4318)
                     /gene="alaS"
                     /locus_tag="VBC74_001419"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEO6942362.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHSVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPEWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYDPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDATAKVQALS
                     DSLRDLQKENVELKEKAAAAAAGDVFKDIQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKGLAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAENL"
     gene            complement(4334..4819)
                     /locus_tag="VBC74_001420"
     CDS             complement(4334..4819)
                     /locus_tag="VBC74_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEO6942363.1"
                     /translation="MATFQIKEKMFSLGGKFTITDLTGLPCYHVEGSLFPLPKTFKVF
                     DEEEHLISQIEKKVLSFLPKFNVTLANGNHFTIKKDFSFLKPHYTIEDLDMEVKGNFW
                     DMDFQLLKDNQVIANISQQWFRMTSTYQVEVYSETYNDLTISLVIAIDYVKELEKNAS
                     N"
     gene            complement(5061..5990)
                     /gene="prsA"
                     /locus_tag="VBC74_001421"
     CDS             complement(5061..5990)
                     /gene="prsA"
                     /locus_tag="VBC74_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEO6942364.1"
                     /translation="MKTRSKLAAGFLTLMSVATLAACSGKTSNGTNVVTMKGDTITVS
                     DFYDQVKTSKAAQQSMLTLILSRVFDTQYGDKVSDKKVSEAYNKTAKGYGNSFSSALS
                     QAGLTPEGYKQQIRTTMLVEYAVKEAAKKELTEANYKEAYKNYTPETSVQVIKLDAED
                     KAKSVLKDVKADGADFAKIAKEKTTATDKKVEYKFDSAGTSLPKEVMSAAFKLDKNGV
                     SDVVSTVDSTTYKTSYYIIKVTDKTEKKSDWKSYKNRLKEVILKDKTSDRSFQNKVIS
                     KALEKANVKIKDKAFAGILSQYATTSGSSSLKK"
     gene            complement(6051..6758)
                     /locus_tag="VBC74_001422"
     CDS             complement(6051..6758)
                     /locus_tag="VBC74_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEO6942365.1"
                     /translation="MVQSYSKNANHNMRRPVVKEEIVQYMRQHQKQNNGCLAELEAFA
                     KQENIPIIPHETATYFRFLMQTLQPKHILEIGTAIGFSALLMAENAPEAKITTIDRNE
                     EMIALAKENFAKYDNHNQITLLEGDAVDVLQTLDKSYDFVFMDSAKSKYIVFLPQVLK
                     HLDVGGVVVLDDIFQGGDIAKPIDEVRRGQRTIYRGLQRLFDSTLQHPDLTATLVPLG
                     DGLLMIRKNADHIVLED"
     gene            complement(6832..7458)
                     /locus_tag="VBC74_001423"
     CDS             complement(6832..7458)
                     /locus_tag="VBC74_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEO6942366.1"
                     /translation="MKKLTFIWDLDGTLIDSYVPIMEALEETYRHFGLIFDKELIHEY
                     ILQESVGQLLVNLSEEEQIPHEKLKAYFTKEQESRDSKIHLMPYAKEILEWTKEQDIP
                     NFMYTHKGASTHSVLETLQISHYFDEILTGVSGFERKPHPQGINYLVKRYSLDKSMTY
                     YIGDRPLDLEVAQNAGIKSINLRLENSKENYNISSLKDIISLDFTRLD"
     gene            complement(7653..9458)
                     /gene="pepF"
                     /locus_tag="VBC74_001424"
     CDS             complement(7653..9458)
                     /gene="pepF"
                     /locus_tag="VBC74_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO6942367.1"
                     /translation="MSDNRSHIEEKYQWDLTTVFATDELWETEVVELTQAIDDAKGFS
                     GHLLDSSQSLLEITEVELELSRRLEKVYVYASMKNDQDTTVAKYQEFQAKATALYAKF
                     SETFSFYEPELLQLSESDYQSFLLEMPDLQKYDHFFEKIFANKPHVLSQNEEELLAGA
                     SEIFGAAGETFEILDNADMVFPVVKNAKGEEVELTHGNFISLMESSDRTVRKEAYQAM
                     YSTYEQFQHTYAKTLQTNVKSQNFKARVHHYQSARQSALSANFIPEEVYETLIKTVNH
                     HLPLLHRYMKLRQKVLGLDDLKMYDVYTPLSQMDMSFTYDEALKKSEKVLAIFGEAYS
                     ERVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRENQPYVYGDYSIFLAEIASTTNENILTETLLKEVKDDKNRFAILNHYLDGFK
                     GTIFRQTQFAEFEHAIHVADQEGQVLTSEYLNNLYAELNEKYYGLTKEDNHFIQYEWA
                     RIPHFYYNYYVFQYATGFAAANYLAERIVNGNPEDKEAYLNYLKAGNSDYPLNVIAKA
                     GVDMTSADYLDAAFRVFEERLVELENLVAKGVHND"
     gene            complement(9474..>9834)
                     /locus_tag="VBC74_001425"
     CDS             complement(9474..>9834)
                     /locus_tag="VBC74_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEO6942368.1"
                     /translation="GDVLAFLKWPYQSKNLNFYKVKQDRNIRDYVRQQLRYGNQFWLR
                     KQEKAYLSGQNLLTQELMMFFPQIQPPRVDTDFCQITNSLTSFYQNFTNYYQKNKNNL
                     DQTLYPPVFYDKIVTKN"
BASE COUNT         2974 a         1935 c         1630 g         3295 t
ORIGIN      
        1 ttttagttaa agcctcttta gccataaact tataaaggcc cgagtaacgt cctctataat
       61 cttttttcca tggtttatct cgtcctgcaa aatgtaaaaa aacagtatgg ttaataaccc
      121 actctaaatc ccattcattg cgacttttta attgataaat aagactatac ctagcatcat
      181 aattataaat ttcatcaggt attggtttaa ccaaattagc gtataagcca tttaagatat
      241 cctgatctgg taagattaga cgaccacggt tttgcattat gtaatctaag atagtttgtt
      301 gatgaacaac ttttcgtata gcaggcaagt tcatcaagag tacccctgta ttgaaataag
      361 aggattctag ctctacattt tttaaccgta atttattgac ataatctaga aacttaccat
      421 cagtattatg acttgccgct gcatatagtt gatcacctaa ctccatgtca tataaacttg
      481 agaaatcatt taagcaaagc atatctgcat ctaaataaag tatcctatct aaggtctcag
      541 gtaaaaattt atgagccaaa agtcgataat atattgtatc tggatagcga tcagttgtag
      601 gtgcctgagc aaatacttcg gtacctacaa ttattggatg atagccaact tctaaatttt
      661 gagtatattg aataagttct gtatgacgtt tgagtaatgt tttttgtaag acatatatct
      721 ctaacttacg attcttggtt tgtcggacaa gcgaataaag catcacttta aaatgatcta
      781 cgtacatatc atcaatagaa aataatagat tcatgagttg gatcctctcc gtagtatatc
      841 ttctttcatc ctaacatatg tctaaagaaa aaccaagttt gagttaaaac ttggttatct
      901 tttatataaa acttccccat aatatagcta aaataattgc tggcaacata ttgataactt
      961 taatatttag gttaaagagt aaattaacac cgacacagaa tataagcata tttcctacta
     1021 agttaaggta atctaaagat gatggattaa gtaggctccc catgaaaaaa gcaacaatcg
     1081 taaggctacc ttgtagtaaa gcgacaggaa gtgcagaaaa tagagcccct ttccctaaag
     1141 acactgtcat aattgcaata ataatcatgt ctaacattcc cttagcaaat aaaatcgaat
     1201 gatctgctgc tataccatct tgaatgctac caacaacggc cattgctcca atacaaacag
     1261 tacaagttga ggtgacgaat gcttccacaa atttaatatc gttaccactt cctgtttttt
     1321 gcttaagata gtccccaaat ctatcaatat acgaatctag tgatagcaac tctccaagta
     1381 ccgtacctag agcaagagta ataatcatca tatttgtatg attagagatg agatgtgact
     1441 tttcgactaa catcattttc tccaaaacac ctgatattga aataaataaa actgcgactc
     1501 ccatggcttg catgaggctc ttttgaagtg attctttcaa aaaatttttc aaaaataaac
     1561 ctacaaagcc tccaactata ataagaataa cattgataac tgttcctaaa ccaaccatat
     1621 ttctcctata tacacaaagg aacagaacga tgttctgccc tttgcctgtt ttatttacta
     1681 actaaatgag aggtacttct tataaatttt ctgcaactgc tgctagcagc tctgcaattt
     1741 tacttgcatc gctaccacct gccatagcca tgtctggttt accaccacca cgacctgcta
     1801 caattggtgc caatcctttg atcatgttac cagcgtggac atctttagtt ttgcttgcaa
     1861 caaggacatt aaccttctca ccaatagctg ctacgagaac aagcacatca gagtagtctt
     1921 tttgtttcca gttatcagca aatgtacgaa gtgcacctgc gtctgcaaca tcaacttgac
     1981 tagcaatgaa gcgcacgccc ttagcttctt gaatatcttt aaagacatca ccagctgctg
     2041 ctgctgctgc tttttcttta agctctacat tttctttttg aagatcacga agtgaatcgc
     2101 taagagcttg tactttagct gttgcatctt tcaattgcgg agcttttaca gtagcagcga
     2161 tctcttttag tgcatcctct tggttacgat aagcttcaaa agcttgtcta ccagtaactg
     2221 caataatacg acgagtgcct gaaccaatac cttcttcttt gacaatcttg aagagaccga
     2281 tttctgaaga attatttaag tgagttccac cgcaaagttc aacagaataa ttaccaattt
     2341 gaaccacacg aaccacttta ccatatttct caccaaaaag tgccattgct cccatctctt
     2401 ttgcggtttc aacgtcagtt tcagtcgttg tgattgtaag agcgttccaa atttgctcat
     2461 taacttcttg ttcaatgtga cgaagttcct cattgcttac tgcttcaaag tgagtaaaat
     2521 caaagcgcaa gaattcttct tcgttcaatg aaccagcctg agttgcgtgt tcaccgataa
     2581 cattgtgaag ggctgcatgg agcaagtgag ttgctgtgtg gtttttctca acagccaaac
     2641 gacgctcttt gttgatttca agtgtgtagt ttgttccaac tgaaagtgat gccaaaacgt
     2701 ttacagtgtg tagaggttga ccatttggtg ctttttgaac atcaacaacc tcagcaactg
     2761 tgtcaccctt atcatttttg attctacctg tgtcagcaac ctgtccaccc atttcagcat
     2821 agaatggtgt ttgagcaaag acaagaaggg cttgaccttc tgaaacagcc tcagtacgtt
     2881 cattatcagc aatgataact gaaagacttg attcaagact atatgtgtcg tattcgaatc
     2941 gtgattcttc aacaatacca gctagggttt cattttgcat ccccatcgaa ccacccttaa
     3001 caacagctgc acgcgcacgg tcttgttgtt ctttcatggc tgacttaaag ccttcgtggt
     3061 caatcttata gcctgcatct tctgccaatt cctctgtcaa ttcaactggg aatccataag
     3121 tatcataaag tttgaagata tctttacctt caagtgtgtc tttaccttca gccttaagct
     3181 gcgcaagcaa tgaatctaag tgaccgctac cagcatcgat agtacgagca aatgtttctt
     3241 cctcacgttt aacgattttc tcaataaaat cacgtttttc aagcacttct ggatagtagc
     3301 tttccatgat ttgtccaaca gtcggaacca atttgtaaag gaaagtttcg ttgatgccaa
     3361 gacggcgacc gtgcataacc gcacgacgga gaagacgacg aagaacataa ccacgacctt
     3421 catttccagg aagcgcacca tcaccgatgg caaatgaaag cgcacggatg tgatcagcga
     3481 taaccttgaa gctcatgttg tcgccatctg gatcgtaagt tttacctgac aatttctcta
     3541 cttcacggat gataggcatg aaaaggtcag tttcaaagtt tgtttttgcc ccttgcataa
     3601 cagctgcaag acgttcaaga cccgcacccg tatcaatgtt tttgtttggc aattctttgt
     3661 attctgaacg tggtacggct gggtcagcat tgaattgtga gagaacaatg ttccagattt
     3721 cgatgtaacg atcgttttcg atatcttcag ccaaaagacg aagtccaata ttttctggat
     3781 caaaatcttc accacggtcg aagaaaatct cagtatctgg acctgaagga ccagcaccga
     3841 tttcccagaa gttatcctcg attggcacca agtgacttgg ttcaacgcca caagcaatcc
     3901 aacggttata tgaatccttg tcatctgggt aataagtcat gtagagcttg tctttaggga
     3961 agtcaaacca ttcaggactt gtcaagagtt caaatcccca ctcaatagct tcatcacgga
     4021 aatagtctcc aattgagaag ttaccaagca tttcaaacat agtatggtga cgtgctgttt
     4081 taccaacatt ttcaatatca ttagtacgaa ttgatttttg tgcattggta atacgtggat
     4141 tttctggaat cactgaacca tcaaaatatt ttttcaaggt tgcaacacct gagttgatcc
     4201 aaagaagcgt tgggtcatta acaggaacca agttagctga aggctcaacg gaatgtcctt
     4261 tggatttcca gaaatccaac cacatttggc ggatttgtgc agacgataat tctttcatta
     4321 ttctctccta aaattagttt gatgcatttt tttctaactc tttgacatag tcgatcgcta
     4381 tgacaagcga aattgtcaaa tcgttgtaag tttcactgta aacttctacc tgataagttg
     4441 aagtcatacg aaaccactgt tgagatatgt ttgctataac ttgattgtct tttaagagtt
     4501 ggaaatccat atcccaaaaa tttcccttaa cttccatatc aaggtcttca atggtgtaat
     4561 gaggtttcaa aaatgagaaa tctttcttaa tcgtgaaatg attcccattg gctaatgtca
     4621 cattaaactt tggtaaaaaa cttaaaacct tcttctcaat ctgactaatg agatgttcct
     4681 cttcgtcaaa gactttaaaa gtcttaggta gagggaacaa acttccttca acgtgataac
     4741 agggaagccc agtcaggtct gtaatagtaa atttaccgcc tagagaaaac attttttctt
     4801 taatttgaaa agtagccata tctcttcctc ctcgaaagtt gacaaatagc aaaagacaag
     4861 tccattacga agggcgaaaa ccgcggtacc accttcattc aatgaacttg tcattctcat
     4921 ttatttttag ttttacccca ttgagtagca tctttttctg attatccatg actcgcagca
     4981 accgtcactt ttctgtatct aatcgttgaa atttcattct caatagtatt aattatacag
     5041 tctttttaag acttggtcaa ctatttttta agtgatgatg aaccacttgt tgtagcatat
     5101 tgtgataaga tgcctgcaaa tgctttatct ttaatcttaa cgttagcttt ttctaaggct
     5161 tttgaaatca ctttattttg gaaggatcta tcgcttgttt tatccttaag aataacttct
     5221 tttaagcgat ttttgtaaga tttccaatca gattttttct ctgtcttatc agttacttta
     5281 atgatgtagt aacttgtttt ataagttgtt gaatcaaccg ttgaaaccac atctgaaaca
     5341 ccatttttat ctagcttaaa ggctgctgac ataacttctt tagggaggct tgtccctgca
     5401 gaatcaaatt tatactcaac ttttttatca gtagctgttg ttttttcttt tgcaatcttt
     5461 gcaaaatcag ctccatcagc ctttacatct ttaaggacag atttagcttt atcttctgca
     5521 tccaatttga ttacttgtac agaagtttca ggagtatagt ttttatatgc ttctttatag
     5581 tttgcttctg ttaattcttt cttagctgct tcttttacag catattccac aagcatagtt
     5641 gtgcgaattt gttgtttgta accttccgga gtcaaacctg cttgtgaaag tgcgcttgaa
     5701 aatgaattac catagccttt agctgtctta ttataagctt ctgatacttt tttatctgaa
     5761 actttatcac catactgtgt atcaaaaaca cgtgagagga tcaatgtaag cattgattgt
     5821 tgtgcagctt ttgatgtttt tacttgatca taaaaatcag agactgtaat agtgtcgccc
     5881 ttcattgtaa caacatttgt tccatttgat gttttacctg agcaggcagc taatgtggca
     5941 actgacataa gtgtcaaaaa gcctgctgca agttttgatc ttgttttcat gatttataac
     6001 tcctttttgt cattaactcc attattatat cataatttct taaaaatgcc ttaatcttct
     6061 agtacaatat gatctgcatt tttacgaatc atcaataaac catctcccaa aggtaccaaa
     6121 gtagcagtta aatcaggatg ttgcaaagtc gagtcaaata aacgttgcag acctcggtag
     6181 atagttcgct gaccccttct cacttcatcg ataggttttg caatatctcc accttggaaa
     6241 atatcatcta aaacaactac tcctccaaca tctaaatgct ttaatacttg aggtaaaaaa
     6301 acgatatatt tagacttagc agaatccata aatacaaaat cgtaagattt atccaatgtt
     6361 tgtaacacat ccacagcatc gccttccaaa agggttatct gattgtgatt atcatatttt
     6421 gcaaagtttt ccttagccaa ggcaatcatc tcttcattac gatcgattgt tgttatcttt
     6481 gcttcaggag cgttttctgc catcaaaagg gccgaaaaac caatagctgt tccgatttct
     6541 aaaatgtgct taggttgtaa tgtttgcatc aaaaaacgaa aataagtcgc tgtttcatga
     6601 ggtataatgg ggatattttc ctgtttagca aatgcttcta actctgctaa acagccatta
     6661 ttttgttttt ggtgctgacg catatattga acaatttctt ctttaactac tggacggcgc
     6721 atattatgat tagcattttt actgtaagat tgaaccactt ttggctcctc ttttctattt
     6781 taaataataa cttttttatt atagcaaaaa atgtctaact ctgagaatga tttaatccaa
     6841 acgagtgaaa tcaagtgata ttatatcttt gagacttgaa atattatagt tttctttgga
     6901 attctctaac cttaagttta tggattttat accagcattt tgagcaacct ccaaatctag
     6961 tggacgatct cctatgtaat aagtcattga tttatctaaa gaatatcgtt taactaaata
     7021 attaatccct tgtggatgtg gttttcgctc gaatcccgaa acaccagtta aaatttcatc
     7081 aaaataatga gagatctgca aggtttccaa cactgaatgc gtacttgctc ctttatgtgt
     7141 atacataaaa ttgggaatat cttgttcttt ggtccattct aaaatctctt ttgcatatgg
     7201 cattaaatgt attttagaat ctcgactttc ttgttctttt gtaaaatatg ctttcagttt
     7261 ttcatgaggt atttgctctt cctctgaaag gtttaccaat aattgcccca ctgattcctg
     7321 taaaatatat tcatggatta attctttatc aaatattaag ccaaaatgac gataggtttc
     7381 ttcaagagct tccataattg gtacatacga atctattaat gtcccatcta aatcccaaat
     7441 aaaagtaagc tttttcatcc tatttccctt cttttttgac aatagcagtt ttggtctcat
     7501 aaaaatcata gcttgggtag gtatttccac ctattgagtt ttccactaac taactttacc
     7561 aaaaccactc tcaaaacaaa ctataaaata actgatcact atcaaaaacc agtctagttg
     7621 actggtttta cttcaatagc tatattttca acttaatcat tatggacgcc ttttgctaca
     7681 agattttcca gttccactaa acgctcttca aatactctaa aagctgcatc cagatagtct
     7741 gcactagtca tatcaacacc ggctttagca atgacgttta gagggtaatc tgaattccca
     7801 gctttaaggt aatttaggta ggcctcttta tcctccggat taccgtttac aatacgctct
     7861 gctaaataat ttgctgctgc gaagccggtt gcatattgga aaacataata attgtagtaa
     7921 aagtgtggaa ttcttgccca ttcatattga ataaaatgat tatcttcttt agtcaaacca
     7981 taatattttt cattaagctc agcgtaaagg ttgtttaagt attcgctagt tagtacttgc
     8041 ccctcctgat cagctacatg aatggcatgt tcgaattcag cgaactgtgt ttgtctaaaa
     8101 attgttcctt tgaaaccgtc caaataatga ttgagaattg caaagcgatt tttatcatct
     8161 ttaacttctt tcaaaagggt ttctgttaat atattttcat ttgttgttga tgcaatttca
     8221 gctaggaaaa tagaataatc accataaaca taaggttgat tttcacgagt aaacgtcgag
     8281 tgtagactat gtccagtttc atgcaccagc gtataaagat tgtctaaagt atcttgccag
     8341 ttaagaagca tgaaagcatt agtgtcataa gatccacctg aataagcgcc tgacctcttt
     8401 cctttgttaa catggacatc aatccaacgt tcagtaaaag cacgatgaac acgttcagaa
     8461 tatgcttcgc caaaaatagc cagaaccttc tctgattttt ttagagcttc atcataagta
     8521 aaactcatat ccatttgaga aaggggtgta tatacgtcat acatcttcaa atcatctagc
     8581 ccaagaactt tttgacgcaa cttcatatac cgatgtaaga gcggtaaatg atgattaact
     8641 gtcttaatta gagtttcgta gacctcttct ggaataaaat tggcagatag agctgattgg
     8701 cgtgctgatt gataatgatg cacacgagcc ttaaaatttt gagatttcac atttgtttgt
     8761 aatgttttag cgtaagtatg ctggaattgt tcatatgtgc tatacatagc ttgatatgct
     8821 tcttttcgaa ccgtcctatc gctagattcc ataagggaaa tgaaattacc atgtgtaagc
     8881 tcaacttcct caccttttgc attttttaca actgggaaga ccatatctgc attatcaagg
     8941 atttcaaatg tctcaccagc tgctccaaaa atctcactag caccagctaa caattcttct
     9001 tcgttttgag ataagacatg aggtttattt gcaaatattt tctcaaagaa atgatcatat
     9061 ttttgcaagt caggcatttc taaaaggaat gactggtaat cagactctga aagttgcaat
     9121 agttctggct cgtaaaacga aaatgtttca ctaaattttg catagagcgc tgtcgctttc
     9181 gcttgaaatt cttgatattt cgcaacagtg gtgtcttggt catttttcat tgaagcatag
     9241 acataaactt tttctaaacg gcgagataac tctagttcaa cctccgtaat ctccaataaa
     9301 gattggcttg agtccaacag atgacctgaa aaaccctttg cgtcatcgat ggcttgagta
     9361 agctctacca cttcagtttc ccataactca tcagtcgcaa agacagtagt taaatcccat
     9421 tggtattttt cttcaatatg tgagcgattg tctgacattt tgtatcctcc agtttaattc
     9481 ttagtgacta ttttatcata aaatacaggg ggataaagtg tttggtccaa gttattctta
     9541 tttttttgat aataatttgt aaaattctga taaaacgatg tcagagaatt tgttatttga
     9601 caaaaatcag tatctacacg tggtggctgt atttgaggaa aaaacatcat tagctcttgg
     9661 gttaatagat tctgaccaga aaggtaggcc ttttcttgtt ttcgtaacca aaattgattg
     9721 ccatagcgta actgctgacg cacataatct ctgatatttc tgtcttgttt aaccttataa
     9781 aaatttagat tttttgattg gtatggccac ttcaaaaatg ctagaacgtc tcca
//
LOCUS       DATROD010000067         9804 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.067, whole genome shotgun sequence.
ACCESSION   DATROD010000067 DATROD010000000
VERSION     DATROD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9804
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.067"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            88..1050
                     /locus_tag="VBC74_001426"
     CDS             88..1050
                     /locus_tag="VBC74_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO6942369.1"
                     /translation="MKKLLTTPVMGCTLVILSLTSLFVGVKSIPLEQITHLNQSQVDI
                     FLASRLPRTISILISGASLSVCGLLMQQLTQNKFVSPTTSGTMDWAKLGVVVTLIFFK
                     NTSIFIQLCIASGFAILGSLLFVTILKMITFKDNIFIPLIGLMLGQIVAAATVFLGTH
                     FQVLQSVNSWLQGNFSIMTSHRYEILYLALPCLFLVYFFAHQFTIVGLGESFAKNLGI
                     AYEKMIYFGLVLVSIMTSLVIIIVGALPFLGLIVPNLISLTKGDHMSSTILETSLLGA
                     CIVMICDLFGRLVIFPYEVSIGVTLGVLGSAFFLISIIRNEGKS"
     gene            1047..2021
                     /locus_tag="VBC74_001427"
     CDS             1047..2021
                     /locus_tag="VBC74_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEO6942370.1"
                     /translation="MIKDLEIPKLLILLILLIATIILFLIYGIPTDANEFLIIYILKT
                     RYQKLIALILVGICIGSSSLIFQTLTNNRLLTPSIIGLDSLYVLIQTGLMYLIGAQRV
                     IKFSSFSSFLLSLLLMVGFAYLLFTILFRNKKQSLYFVLLAGLIFNTLFSSISSFIQA
                     IMDPNDFMILQNQLFASFNAINTKILWISFIIIVVSFVINWPFIKELDVLLLGKENAI
                     SLGISYQKLTTRIFLWLALMVAIATALVGPITFLGLLVAHITYHSFHTFRHQILVPIA
                     IVICIFTLVLGQHLVQNLLHLTVQLSVLLNLIGGSYFIFTLIKGRKNL"
     gene            2018..2779
                     /locus_tag="VBC74_001428"
     CDS             2018..2779
                     /locus_tag="VBC74_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6942371.1"
                     /translation="MIQINNLHKFYGQKEILKDINISIPKGKVTAILGPNGSGKSTLL
                     SCISRLEPYDNGEIFLDKVPLAHYSSNDLAKTLAILRQSNHLTLKIKVSDLIGFGRFP
                     YSKGRLSQKDKAVIESVISYMDLNDIADEFINNLSGGQIQRAFIAMTMAQDTQYICLD
                     EPLNNLDMKYAVQMMDLIKRYAYEFNKTIVIIIHDINFATHYADNVVALKEGQVVSCG
                     TVEDVMQEKILSHLFDMPIRIETVDGKPIILFCTT"
     gene            2841..3869
                     /locus_tag="VBC74_001429"
     CDS             2841..3869
                     /locus_tag="VBC74_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6942372.1"
                     /translation="MTKKLIIAILALCTILTTSQAVLAKEKSQTVTIKNNYSVYIKKE
                     KRDKPDNKKQISETLKVPLKPKKVVVFDMGALDTITALGAEKSVIGIPKAKNALSLLP
                     NNVKSVYKAKRYQDVGSLFEPNFEAIARMQPDVVFLGARMASVDNIEKLKEAAPKAAL
                     VYAGVDSKKVFDKGVAERVTMLGKIFDQNKKAKTFNKDIAQAVLKLQKTIEKKGKPTA
                     LFVMANSGELLTQSPSGRFGWIFSVGGFKAVNENEKLSSHGTPVSYEYIAEKNPNYLF
                     VLDRGATIGQGASSKELFNNDVIKATDAVKNKRVHEVDGKDWYINSGGSRVTLRMIKD
                     VQNFVDNR"
     gene            4007..4849
                     /gene="dprA"
                     /locus_tag="VBC74_001430"
     CDS             4007..4849
                     /gene="dprA"
                     /locus_tag="VBC74_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEO6942373.1"
                     /translation="MNHFELFKLKKAGLTNLNINNIINYLKKNSLTSLSVRNMAVVSK
                     CKNPTFFIENYKQLDLKKLRQEFKKFPVLSILDSNYPLELKEIYNPPVLLFYQGNIEL
                     LSKPKLAVVGARQASQIGCQSVKKIIKETNNQFVIVSGLARGIDTAAHVSALKNGGSS
                     IAVIGSGLDVYYPTENKKLQEYMSYNHLVLSEYFTGEQPLKFHFPERNRIIAGLCQGI
                     VVAEAKMRSGSLITCERALEEGREVFAIPGNIIDGKSDGCHHLIQEGAKCIISGKDIL
                     SEYQ"
     gene            4944..7064
                     /gene="topA"
                     /locus_tag="VBC74_001431"
     CDS             4944..7064
                     /gene="topA"
                     /locus_tag="VBC74_001431"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEO6942374.1"
                     /translation="MATTTKTSTKKTSKKKSATAKKNLVIVESPAKAKTIEKYLGRNY
                     KVVASVGHIRDLKKSSMSIDFENNYEPQYINIRGKGPLINDLKKEAKKAKKVYLASDP
                     DREGEAISWHLAHILDLDKEDRNRVVFNEITKDAVKNAFVEPRQINMDLVDAQQARRV
                     LDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENEIKAFQPEEYWTIDGSFKK
                     GTRKFNATFYGLDGKKFKLSNNEDVKTVLKRIKTDEFLVEKVEKKERRRNAPLPYTTS
                     SLQQDAANKINFRTRKTMMIAQQLYEGLSLGTAGHQGLITYMRTDSTRISPLAQNEAT
                     EFITNRFGANYSKHGNKVKNASGAQDAHEAIRPSSVNHTPESIAKYLDKDQLKLYTLI
                     WNRFIASQMTAAVFDTMKVNLTQNGVTFIANGSQVKFDGYMAVYNDTDKNKMLPDMEE
                     GESVKKVNTNPEQHFTQPPARFSEASLIKTLEENGVGRPSTYAPTLETIQKRYYVKLA
                     AKRFEPTELGEIVNSLIVEFFPDIVDVTFTAEMEGKLDEVEIGKEQWQKIIDEFYKPF
                     EKELAKAETEMEKIQIKDEPAGFDCELCGSPMVIKLGRYGKFYACSNFPECHNTKAIT
                     KEIGVICPICQKGQVIERKTKRNRIFYGCDRYPECEFTSWDKPIGRTCPKSNDFLVEK
                     KVRGGGKQVVCSNEKCDYQEEKIK"
     gene            7201..7902
                     /locus_tag="VBC74_001432"
     CDS             7201..7902
                     /locus_tag="VBC74_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6942375.1"
                     /translation="MSYIEMKHSYKRYQTGETEIVANNDISFSIERGELVVILGASGA
                     GKSTVLNILGGMDSNSEGEVLIDGKNIANYTIRELTRYRRYDVGFVFQFYNLVPNLTA
                     LENVELASEIVPKALDAQQALENVGLGHRINHFPAQLSGGEQQRVAIARAIAKKPKLL
                     LCDEPTGALDYQTGKQVLAILQKMAQSKETTVIIVTHNTALAPIANRVIHMHDSKISD
                     IVINENPSDIQNIEY"
     gene            7914..>9804
                     /locus_tag="VBC74_001433"
     CDS             7914..>9804
                     /locus_tag="VBC74_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6942376.1"
                     /translation="MGKTFWKDIYRSITTSKGRFSSILLLMMLGSFAFIGLKVSAPNM
                     QRTAQNYLAHHHVMDITVFNSWGLDKHDQTVLESLKGSQVEFSYFVDTTPQQNSKSYR
                     LYSNTKTISTFDLVKGRLPLNKSEIALSFQERKKYAIGDKINFKQDKNKLFSNTGPLT
                     IVGFVNSTEIWSKTNLGSSQTGDGDLDSYGVLDKTAFHSPVYTMARVTFKDLRLINPF
                     SISYKEKVAKYQEKVSRKLNIHNKIRYTKTKKESLRKIDEEEKSLLKAQKQINRLDND
                     SLAMPLSQRQAIQMKIKQDRLSLLKRTKELLKLRHNTQIMESPQIIVYNRTTFPGGQG
                     YNTFDSSTNSTSKISNLFPIILYLVAALVTLTTMTRFVEEERTNAGILKALGYSDRQV
                     IFKFIIYGFIAGTLGTTLGIIGGHYLLPRIISDIISKDLTIPNTQYHLFLNYSLLAFV
                     FSLLSIVLPVFVITRRELKEKAAFLLLPKPPAKGSKIALEYINWIWKKLSFTQKVTAR
                     NIFRYKQRMIMTIFGVAGSVALLFSGLGIQSSLKQTVNEHFGRIMPYDILLTYNTNAS
                     PPKILELLSKDSKIDKYQPIHLENLDESIPGQINKQSISLFITDKKQLLPFIYLQEAT
                     TNKSLH"
BASE COUNT         3347 a         1646 c         1699 g         3112 t
ORIGIN      
        1 gaaaaagtag atatttttcc aaaaaattat gttattatag atttgtactc atttttgaac
       61 actttatctt tcattttaag gagttttatg aaaaagctat taaccactcc tgtaatgggt
      121 tgtactttgg ttatactttc tctaacgtcc ctatttgttg gtgttaaatc tatcccactg
      181 gagcaaataa cacaccttaa tcaatcacag gtagatattt ttttagcgag tcgcttacca
      241 agaaccatta gtatcctaat atctggtgca agtttgtcag tctgtggcct gctaatgcaa
      301 caactcaccc aaaacaagtt cgtttcaccg acaacttcag ggactatgga ttgggcgaaa
      361 ttaggagtag ttgttacgct gattttcttt aaaaatacct ctatttttat tcaactatgt
      421 attgcttcag gttttgccat tttaggatcg ctgctttttg taactatttt aaaaatgatt
      481 acttttaagg ataatatttt tatacctctt atcggactga tgcttggaca aattgtagca
      541 gcagcaacag tatttttagg aacacatttt caagtactac aatctgtcaa cagttggctt
      601 caaggtaatt tttccatcat gactagccat cgctacgaaa ttctttactt agcattgcca
      661 tgtttgtttt tagtgtattt ttttgctcat cagtttacca ttgttgggct tggagaaagc
      721 ttcgctaaaa atttagggat tgcttatgaa aagatgattt actttggttt agttttggtt
      781 tcaattatga catccctagt cattattatc gtaggagctt tacctttctt aggtttaatc
      841 gtacctaatc taattagctt aactaaaggg gatcatatgt cctcaactat cttagagaca
      901 tcgcttttag gggcttgtat cgtcatgata tgtgatcttt ttggtcgctt agttattttt
      961 ccttatgaag tctcaatagg agttacgcta ggagtattgg gtagtgcctt tttcttgatt
     1021 tcaattataa gaaatgaggg aaaatcatga ttaaggattt agagatacca aaattactta
     1081 ttttactgat actactaatt gctaccatta ttttgttttt aatttatggt attccaactg
     1141 acgcaaatga atttttgatt atctatatcc taaaaacacg ttatcaaaaa ttgattgcat
     1201 taattttggt tggtatctgc attggtagtt ctagtcttat ttttcaaact ctaaccaata
     1261 accgactttt aactccgtcc attattggtt tagatagctt atatgttctt atccaaacag
     1321 gattaatgta tttaatcgga gctcagcgtg tcataaaatt ttcatctttt agcagcttcc
     1381 tcctttcatt attattaatg gttggattcg cttacttatt atttacgata ctttttcgaa
     1441 ataaaaaaca gtctttgtat tttgttctat tagctggact catatttaat acattatttt
     1501 cttctatctc atcctttatt caagccatta tggaccccaa cgattttatg attttacaaa
     1561 atcagctctt tgctagtttc aatgctatta ataccaagat cctttggatt tctttcatta
     1621 tcattgtcgt tagctttgtc attaattggc cttttataaa ggaattggat gtcttactat
     1681 tgggtaaaga aaatgcgata tcactcggca tctcatatca aaagcttact actagaattt
     1741 ttttatggtt agcattgatg gtagctatag caacagcatt agttgggccg attacttttt
     1801 taggcttact tgtagcacat attacctatc acagttttca tacctttaga catcaaatat
     1861 tagttcctat agccattgtt atctgtatct ttacacttgt cttagggcag catctcgttc
     1921 aaaatctatt acacctcact gttcaactaa gtgttctcct caaccttatc gggggaagtt
     1981 attttatatt caccttaatc aaaggaagga aaaacttatg attcaaataa acaatttaca
     2041 taagttctat ggccaaaagg aaatattaaa agacattaat atttcgattc ctaaaggaaa
     2101 agtaacagct attttaggtc caaatggatc tggaaagagt acattgctga gttgtatcag
     2161 tcgacttgaa ccttatgata atggtgaaat tttccttgat aaagtaccat tagcccacta
     2221 cagttcaaat gacttagcca aaacattagc catattaaga caatctaatc acttaacact
     2281 caagataaag gtgagtgact tgataggatt cggtagattt ccttatagta aaggacggtt
     2341 aagtcaaaaa gataaagctg tcatagaatc agttatatct tatatggatt taaacgatat
     2401 agctgacgag ttcattaata acctcagcgg aggtcaaatt caaagagctt ttatcgcaat
     2461 gacaatggca caagatactc aatatatttg cttagatgag ccactcaaca atttagatat
     2521 gaaatatgct gttcaaatga tggatcttat caaacgatat gcgtatgagt ttaataaaac
     2581 tatagtgatt attattcacg acatcaattt tgcaactcac tacgctgaca atgttgtcgc
     2641 tttaaaagaa ggacaagtgg tgtcttgtgg gactgtcgaa gatgttatgc aagaaaaaat
     2701 cctttctcat ttatttgata tgcctatccg tatcgaaact gttgatggta aaccaattat
     2761 ccttttttgt acaacgtaaa taaagagatt tatgtaggat aacattttta gtttttatct
     2821 attttaggag attttattta atgacaaaaa aacttattat tgctatatta gcactatgca
     2881 ctatcttaac cacttctcaa gctgttttag ctaaagaaaa atcacaaact gttaccataa
     2941 aaaacaacta ttcggtctat attaaaaaag aaaaaagaga caagccggat aataaaaagc
     3001 aaatcagcga gacacttaaa gttcctttaa aacccaaaaa agtagttgtt tttgatatgg
     3061 gagctttgga tactatcaca gctttaggag ctgaaaaatc tgttattggt atcccgaagg
     3121 ctaaaaatgc tctaagttta ttgcccaata acgtcaaatc tgtttataaa gctaagagat
     3181 accaagacgt aggaagtctc ttcgaaccaa actttgaagc tattgctcgt atgcaacctg
     3241 atgtggtttt cctaggagca cgtatggctt ctgttgataa tattgaaaaa ttaaaggagg
     3301 ctgcacctaa agcagcatta gtatatgctg gagtcgactc aaaaaaagta tttgacaaag
     3361 gagttgctga gcgtgtcaca atgttaggga aaatcttcga ccaaaataaa aaggcaaaaa
     3421 cctttaataa agatatcgca caagctgttc ttaaattgca gaaaactatt gagaaaaaag
     3481 gtaaacctac agctctattt gtaatggcaa acagcggtga acttttaact caatcacctt
     3541 ctggtcgttt tggttggatt ttctctgtag gtggatttaa agcagtcaat gaaaatgaaa
     3601 aactaagttc acatggtact cccgtatctt atgaatacat cgctgaaaaa aatcctaact
     3661 atctctttgt tttagatcgt ggagcgacta ttggacaagg agcttcatca aaagaacttt
     3721 ttaataacga tgttattaaa gcaactgatg ctgtcaaaaa caaacgtgtt catgaggtag
     3781 atggaaaaga ttggtatatc aattcaggcg gaagccgagt aacactccgt atgattaaag
     3841 atgtacagaa ctttgttgat aatcgttaat tttttgtcaa aggctaggaa aaatcctagt
     3901 cttatttttt tgaaaaaata gctattatca atgcttcaga gtatagattg atcttatcac
     3961 tttattttgt ctattatttt cgaatatagt caatggaggt aaccttatga atcattttga
     4021 actatttaag cttaaaaaag ctggactaac aaatcttaat atcaacaata ttatcaacta
     4081 tctcaaaaag aatagtttaa cttctctatc tgttcgcaat atggccgtag tatcaaaatg
     4141 taaaaatcct actttcttta tagaaaatta taaacagcta gaccttaaaa aacttcgaca
     4201 agaattcaaa aaatttccag ttctatcgat tttggattct aactatcctt tagagttaaa
     4261 agaaatatat aatccaccag ttctactttt ttatcagggt aatattgaac ttctatctaa
     4321 acctaaatta gctgtagtgg gggcaagaca ggcatctcag ataggttgtc agtctgttaa
     4381 aaagattatc aaggagacta acaatcaatt tgttatcgta agtggtttag cgcgtggcat
     4441 tgatacagca gcacatgtta gtgctttaaa aaatggcggc agtagtatag ctgttatcgg
     4501 gagtggttta gatgtttatt atccaacgga gaataagaaa cttcaagaat atatgtcata
     4561 taatcatctc gtattatcag aatattttac cggagaacaa cccttgaaat ttcatttccc
     4621 cgaacgtaac cgtattattg cagggctgtg tcaaggtatt gtggttgccg aagctaagat
     4681 gagatctgga agtttaatta cctgtgaaag agcattagaa gagggacgag aagtttttgc
     4741 cattcccgga aatattatcg atggcaaatc agatggatgc caccatctta tccaagaagg
     4801 agctaaatgc attatttcag gaaaagatat cctttctgaa tatcagtagt gttttgcttt
     4861 ctactatagg agaagttttc tattgacagt ttaagaaaag tgagttatta tgctataagt
     4921 ttacgatata gaaagtgata cctatggcaa ctacgacaaa aacgtcgacc aaaaaaacaa
     4981 gtaaaaaaaa atcagctact gctaaaaaaa atctagttat tgtggagtct cctgcaaaag
     5041 caaagactat cgaaaaatac ttaggacgta actataaagt tgtagcctca gtaggtcata
     5101 tccgtgattt aaaaaagtca agtatgtcca ttgattttga gaataattac gaaccacaat
     5161 atattaatat tcgtggtaaa ggacctctca ttaatgattt aaaaaaagaa gctaaaaagg
     5221 ctaaaaaagt ttacctcgcg agtgacccgg accgtgaagg agaagctatt tcctggcatt
     5281 tagcgcatat tttagattta gacaaagaag acagaaaccg tgttgttttc aatgaaatca
     5341 caaaagatgc tgttaaaaat gcttttgtgg aacctcgtca gattaacatg gatctcgttg
     5401 atgctcagca agcacgacgt gttttagatc gcattgttgg ttattctatc tcacctattt
     5461 tatggaaaaa agtaaaaaaa gggctatcag ctggacgtgt acaatcagtt gctcttaaac
     5521 taatcattga tcgtgaaaat gagattaagg ctttccaacc ggaggagtat tggactattg
     5581 acggttcctt taaaaaagga acacgtaaat tcaatgccac tttctacggt ttagacggaa
     5641 agaaattcaa attatcgaat aatgaagacg tcaaaacagt tctaaaacgt attaaaactg
     5701 atgaattctt agttgaaaaa gttgaaaaaa aagagcgtcg tcgtaatgca ccattaccgt
     5761 atacaacttc ttcattgcaa caagatgcag ctaataaaat caattttcga actcgtaaaa
     5821 ccatgatgat tgcgcaacag ctgtacgaag gacttagctt aggcacagca ggtcatcaag
     5881 gtctgattac ctatatgcgt actgattcta cacgtattag tccgttagca caaaatgaag
     5941 caacagaatt tattactaac cgttttggtg caaattattc taaacatggg aataaagtta
     6001 aaaatgcttc tggagcacaa gacgctcacg aagctattcg cccatctagt gttaatcata
     6061 cacccgaaag tattgctaag tatttagaca aagatcaact aaaactttac actcttatct
     6121 ggaaccgttt tattgcaagc caaatgacag cagcagtctt tgacacaatg aaagttaatt
     6181 taacgcaaaa tggtgttact tttattgcta atggtagcca agtcaagttt gatggttaca
     6241 tggctgttta taacgatact gacaaaaata aaatgttacc agatatggag gaaggagaaa
     6301 gtgttaaaaa ggttaatacg aatcctgaac aacacttcac tcaacctcct gcaaggtttt
     6361 cagaagcaag tctcattaaa acacttgaag aaaacggtgt aggtcgtcct tcaacttatg
     6421 ccccaacgct tgagactatt caaaaacgtt attatgttaa gcttgcagct aaacgcttcg
     6481 aaccaactga acttggtgaa attgttaata gccttattgt agaatttttc ccagatattg
     6541 ttgatgttac cttcactgcc gaaatggaag ggaaactaga cgaagttgag attggtaaag
     6601 agcagtggca aaagattatc gatgaatttt ataaaccatt tgaaaaagaa cttgcaaaag
     6661 cagaaactga aatggagaaa attcaaataa aggatgaacc tgctggattt gattgcgagc
     6721 tatgtggatc accaatggta ataaaactag gacgttatgg aaagttttat gcgtgtagca
     6781 atttccctga atgtcataac acaaaagcta tcactaagga aataggtgtt atttgtccta
     6841 tctgtcaaaa aggacaagtt attgagcgaa aaacaaaacg caatcgtatc ttttatggct
     6901 gtgaccgcta cccagaatgt gagtttacat cttgggacaa acctattgga cgaacttgcc
     6961 ccaaatcaaa tgatttctta gtggagaaaa aagtacgcgg tggtggtaaa caagttgttt
     7021 gctcaaatga aaaatgcgac taccaagaag agaaaattaa ataaagtgga atctgagtgt
     7081 ttagctcaga tttttaaaaa aattattatt ttacataact taatttatgt atttattttt
     7141 atgggagaat taataaaaaa gtgctataat ataggtgtat tcatagtgtg aggtgccgtt
     7201 atgtcctata ttgaaatgaa gcattcctat aagcgttatc aaacaggtga aaccgaaatt
     7261 gttgctaaca atgatattag tttcagtatt gaaagaggtg agttggtggt tattcttggt
     7321 gcatccggcg ctggcaaatc tactgttctc aatatactag gagggatgga tagtaatagt
     7381 gaaggtgaag ttttaattga tggtaagaat attgctaatt ataccatcag ggaattgact
     7441 cgttaccgta gatatgatgt cgggtttgtt tttcaattct ataacttagt ccctaatcta
     7501 acagcactag agaatgtaga attggcttca gaaattgttc caaaggcatt agatgcccaa
     7561 caggcattag aaaacgtagg tttgggacac cgtatcaatc attttccagc acagctttcc
     7621 ggtggcgaac agcaacgcgt tgcaatagca cgagctattg ccaaaaaacc taaactgtta
     7681 ctttgtgatg aacctactgg tgctttagat taccaaactg gaaaacaagt actagccatc
     7741 ctgcaaaaga tggcgcaatc aaaagaaaca actgtgatca ttgttactca taatactgcc
     7801 ttagcgccaa ttgcaaatcg cgtgatccat atgcatgatt caaaaatcag tgatattgta
     7861 ataaacgaga atccaagtga tattcagaac atagaatatt agaggttata cagatgggaa
     7921 agacattttg gaaagatatc tatcgatcaa ttacgacttc aaaaggtaga ttttcatcaa
     7981 tcctcttact tatgatgtta ggctcatttg cttttatagg actgaaggtc tcagcaccta
     8041 atatgcaaag aacagcacaa aattaccttg ctcatcatca tgttatggat attaccgttt
     8101 tcaattcttg gggacttgac aaacatgatc aaactgtttt agaaagctta aaaggatcac
     8161 aagttgaatt ttcttacttt gtagatacaa cccctcaaca gaatagtaaa tcttatcgat
     8221 tgtattccaa tactaaaacg atatcaacct ttgatcttgt taagggacgt ctccctttaa
     8281 ataaatcaga aattgctctc tcatttcagg aacgaaaaaa atatgcaata ggtgataaaa
     8341 ttaattttaa acaagataaa aacaaactgt tttcaaatac aggtcctcta actattgtag
     8401 gttttgttaa ttctaccgag atttggtcta agacaaacct tggtagttct caaacagggg
     8461 acggcgactt agatagttat ggcgtattag ataagacagc atttcactct ccagtatata
     8521 caatggcaag ggtaactttt aaagatctta ggttgattaa ccctttctca ataagttaca
     8581 aagaaaaagt agcgaaatac caagaaaagg tatcgcgaaa attaaatatt cacaataaaa
     8641 taagatatac caaaacaaaa aaagagagcc tacgtaaaat cgatgaggag gaaaaaagcc
     8701 tactaaaagc tcaaaaacaa atcaatcggt tagataatga tagcctagcg atgccactat
     8761 ctcaaagaca agctattcaa atgaaaatta aacaagaccg actatctctt ttgaaacgaa
     8821 caaaagagct tctaaaattg agacataaca cccaaataat ggaatcgcct caaattattg
     8881 tctacaaccg tactaccttt cctggaggac aaggatataa cacttttgac tccagtacaa
     8941 atagcacttc taaaatcagt aatcttttcc ccattatttt atatttagtc gcagcattag
     9001 taaccttaac aacaatgact aggttcgtcg aggaagaaag aaccaacgca ggcatattaa
     9061 aagcactagg ctatagtgac cgccaagtta tctttaaatt tattatttat ggttttatag
     9121 caggaacatt agggactact ttgggtatca ttggagggca ttatctttta cctcgtatta
     9181 tttctgatat catttccaaa gacctgacta ttccaaacac acagtatcat cttttcttaa
     9241 attacagttt actagccttt gttttttcat tattaagcat tgttcttcca gtttttgtta
     9301 tcactcgacg tgagttgaaa gaaaaagcag catttttatt attaccaaaa ccaccagcta
     9361 aaggatctaa aatagcttta gagtatatca attggatttg gaaaaaactg tccttcacac
     9421 aaaaagtaac tgctagaaat atttttcgtt acaaacaaag gatgattatg accatatttg
     9481 gagttgctgg atcagtagcg ctcttatttt caggtctagg aatacaatcc tccttaaagc
     9541 aaaccgtcaa tgaacatttc ggtcgtatta tgccctatga tatattgcta acatataaca
     9601 caaatgcttc tcccccaaaa atacttgaac tactttcaaa agattcaaaa atagacaaat
     9661 accaaccaat acaccttgaa aatcttgacg aatccattcc tggacaaatt aataaacagt
     9721 caatttccct attcataaca gacaaaaaac aattactacc ttttatttat ttacaagaag
     9781 caacaactaa taagtcattg cact
//
LOCUS       DATROD010000068         9740 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.068, whole genome shotgun sequence.
ACCESSION   DATROD010000068 DATROD010000000
VERSION     DATROD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9740
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.068"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..408
                     /locus_tag="VBC74_001434"
     CDS             <1..408
                     /locus_tag="VBC74_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HEO6942377.1"
                     /translation="KVDSSNIIIAMHFVPHKQFTMTHPRFSPFNAFLGSQAYHDLFQK
                     YHIKDVVFGHAHRSFGDVKIGETTYHSRPLGYIREWNLTIDFVNQNPNHNPNLTWNLS
                     KRHNAVKHLDSFENYPKKYFEDELRNSMTIFDC"
     gene            complement(453..3137)
                     /locus_tag="VBC74_001435"
     CDS             complement(453..3137)
                     /locus_tag="VBC74_001435"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HEO6942378.1"
                     /translation="MLKEQKKSLFYTQGQEEVLTSLESSREGLSTTEAKNRLEMYGRN
                     ELEEGKKRSLIAKFFDQFKDLMIIILLVAAALSVITEGMHGLTDALIILAVVILNAAF
                     GVYQEGQAEAAIEALKDMSSPIARVRRDGHTIEVDSKELVPGDLVMLEAGDVVPADLR
                     LLEAASLKIEEAALTGESVPVEKDISQVVAEDAGIGDRVNMAYQNSNVTYGRGYGVVT
                     NTGMYTEVGKIADMLANADESETPLKQSLVQLSKLLTYLIVIIAVITFLVGIFVRKEG
                     WIEGLMTSVALAVAAIPEGLPAIVTIVLSMGTKTLAKRNSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGVLQSSSEEISVDNNTLRIMNFSNDTKIDPSGKLIGDP
                     TETALVQFGLDKNFDVREVLKNEPRVAELPFDSDRKLMSTIHKESDGRYFIAVKGAPD
                     QLLKRVTKIEDNGLVRDITAEDKEAILNTNKELAKQALRVLMMAYKYETQIPTLETDI
                     VESDLVFSGLVGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGIIDA
                     NDTEDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNDGKVVAMTGD
                     GVNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKS
                     IQYLLSANMAEVFTIFFATLLGWDVLAPVHLLWINLVTDTLPAIALGVEPAEPGVMTH
                     KPRGRQSNFFDGGVMGAIIYQGILQTILVLGVYGWALMYPEHAGYRMIHADALTMAFA
                     TLGLIQLVHAFNVKSVYQSIFTVGAFKNRTFNWSIPVAFILLMVTIVVPGFNKLFHVT
                     HLSSTQWLTVVIGSLLMVVLTEIVKFIQRKLGQDEKAI"
     gene            complement(3481..3879)
                     /locus_tag="VBC74_001436"
     CDS             complement(3481..3879)
                     /locus_tag="VBC74_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEO6942379.1"
                     /translation="MREVMKLEINNNIQIDNETEMIHEIHDCQFIEKGSYVYLNYINA
                     EGERVVIKANHEELLMTRFSNPKSVMRFHRETPALVNIPTPLGIQHLITETSHYQFDL
                     SQQRLHINYVLKQTETGACFANYELKIQWY"
     gene            3943..5259
                     /locus_tag="VBC74_001437"
     CDS             3943..5259
                     /locus_tag="VBC74_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEO6942380.1"
                     /translation="MNEKVFRDPVHTYIHVNNQVIYDLINTKEFQRLRRIKQTSTTSF
                     TFHGAEHSRFSHCLGVYELARKVTEIFDEHYSDLWNKNESLLTMAAALLHDIGHGAYS
                     HTFERLFNTDHEAYTQEIITNPTTEINAILRKVAPDFPDKVASVINHSYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYYTAASYGQFDLTRILRVIRPTDSGIAFARNGMHAVEDYIVS
                     RFQMYMQVYFHPASRAMELLLQNLLKRARFLFDTHRDFFEQTSPNLIPFFTDQYDLQD
                     YLALDDGVMNTYFQSWMQADDNILADLANRFINRKVFKSITFEEADKENLVKMKELVS
                     QVGFDPDYYTGVHANFDLPYDVYRPEHSNPRTEIQIIQKNGQLAELSSLSPIVKALTG
                     SNYGDQRFYFPKEMLTLDSLFSSTKEEFQSYITNEHLTLTKDNSHS"
     gene            5331..6143
                     /gene="yidA"
                     /locus_tag="VBC74_001438"
     CDS             5331..6143
                     /gene="yidA"
                     /locus_tag="VBC74_001438"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEO6942381.1"
                     /translation="MTSIKLVAVDIDGTLLNSKREITPEVAKAVQEAKSKDVKIVIAT
                     GRPIIGVQDLLEELKLNEEGDYVITFNGGLVQDTATGDDIIKETLTYEDYLDFELLAR
                     KLGVHMHAITKEGIYTANRDIGKYTIHEATLVNMPLFYRTPEEMGDKEIIKLMMIDQP
                     DILDAAIAKIPKKVLDNYTIVKSTPFYLEILPKNVNKGTALLHLAEKMGLTVDQTMAI
                     GDEENDRAMLEVVGNPVVMQNGNPELKKIAKYITKSNEESGVAYALREWVIN"
     gene            6128..7358
                     /locus_tag="VBC74_001439"
                     /pseudo
     CDS             6128..7358
                     /locus_tag="VBC74_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
     gene            7371..8582
                     /locus_tag="VBC74_001440"
     CDS             7371..8582
                     /locus_tag="VBC74_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6942382.1"
                     /translation="MYREITAVEHDRFVSESNQTNLLQSSNWPKVKDNWGSQLLGFFD
                     GETQIASASILIKSLPLGFSMLYIPRGPIMDYSNLDIVTKVLKDLKAFGKKQRALFIK
                     CDPLIYLKMVNAKDFENSPDEKEGLIAIDHLQRAGADWTGRTTDLAHTIQPRFQANLY
                     ANQFGLDKMSKKTRQAIRTSKNKGVDIQFGSHELLEDFAELMKKTEDRKGINLRGIDY
                     YQKLLDTYPNNSYITMASLDVAKRLEKIEKECQIAQSERIKSLELNREKKVKQHQDTI
                     DRLNKEIDFLKEAQKAYDRDIVPLAATLTLEFGNTSENIYAGMDDYFKSYSAPIYTWF
                     ETAQRAFERGNIWQNMGGIENDLSGGLYHFKSKFEPIIEEFIGEFNIPVNRLLYKASN
                     YVYALRKKRNS"
     gene            8601..>9740
                     /locus_tag="VBC74_001441"
     CDS             8601..>9740
                     /locus_tag="VBC74_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEO6942383.1"
                     /translation="MALKELTAKEFESYSGNYDLQSFMQTPEMAKLLKKRGYDITYMG
                     YQIDGKMEIISIVYTIPMTGGLHMEVNSGPAHSNSKYLKHFYKELQNYAKSQGALELL
                     IKPYDTYQEFTGEGEPKGAPNTYLIDGLTSIGYHHDGLHIGYPGGEPDWHYVKNLEGI
                     TPQNLLKSFSKKGRPLVKKAMSFGIKIRVLKREELHIFKDITSSTSDRRDYMDKSLDY
                     YQDFYDSFGDKAEFVIATLNFREYDHNLQLNAKKLEEQITVLDNRHQNNTDSAKYHRQ
                     RTELVNQLASLDKRRKEVEPFIQKFGNQDVVLAGSLFIYSPKETVYLFSGSYTEFNKF
                     YAPAVLQEYVMQEALKRQSTFYNFLGIQGNFDGSDGVLRFKQNFNG"
BASE COUNT         3233 a         1897 c         1637 g         2973 t
ORIGIN      
        1 aaagtcgatt cttcaaatat cattattgct atgcactttg ttccccataa acagttcacg
       61 atgactcatc ctagattctc tccttttaat gcttttttgg ggagtcaggc ttatcatgat
      121 ttattccaaa aatatcatat caaagatgtt gtttttggtc atgcacaccg ctcatttggc
      181 gacgttaaaa ttggagagac tacttatcat tctagaccac ttggatatat tagagaatgg
      241 aatttaacta ttgactttgt caatcagaat cccaatcata accctaattt aacctggaat
      301 cttagcaaaa ggcataatgc cgttaagcat ttggatagtt ttgaaaacta tccaaaaaaa
      361 tattttgaag acgaattacg caactctatg acaatatttg attgctaaaa aactcactta
      421 gatattctaa gtgagttttt ttatcagcta aactaaatag ctttttcgtc ttgtcctaat
      481 ttacgttgga taaatttaac aatttccgtt aacactacca tcaataaact tccgataact
      541 acagttaacc attgtgttga acttaaatga gttacatgga aaagtttatt aaatccagga
      601 acaacaatag tcaccatcaa aagaatgaaa gctactggaa tagaccagtt aaaggttcta
      661 tttttaaaag ctccaactgt aaagattgat tggtaaactg atttaacgtt aaatgcatgt
      721 accaattgga ttaaaccaag cgttgcaaag gccatcgtaa gggcatctgc gtgaatcatg
      781 cgataaccag catgttctgg atacatcaat gcccaaccat agacaccaag tactaagata
      841 gtttgtaaaa taccttgata gataatagct cccataacac caccatcaaa gaagtttgac
      901 tggcgaccac gaggtttatg tgtcataact cctggttcag ctggctcaac tccaagagcg
      961 atagctggaa gagtatctgt taccaagtta atccaaagaa ggtgaaccgg tgcaagtaca
     1021 tcccatccta ataaagttgc aaagaaaatt gtaaatacct ctgccatgtt ggctgaaaga
     1081 agatattgaa tagatttttg gatatttgag aagactttac gtccttcctc aacagccaca
     1141 ataatagttg caaaattatc atcagcaaga accatatcag aagcaccttt ggaaacttct
     1201 gtaccagtta ttcccatacc aataccaata tctgctgttt ttagcgatgg agcatcatta
     1261 acaccatcac cagtcatggc aacgactttg ccatcgtttt gccaagcttt cacgatacgc
     1321 actttatgtt caggagaaac acgtgcatag acagaatatt gtttaaatac tttttgaaac
     1381 tcttcgtcac ttaattcatt aagttcagca cccgtaaaga catgatcttc tgtgtcattt
     1441 gcatcaataa tacctagacg ctttgcaata gcttctgcag tatcttgatg gtccccggta
     1501 atcataatcg gacgaattcc tgcttcttta gcaactcgaa cagcttctgc cgcttctgga
     1561 cgctcagggt caatcattcc cactaaacct gagaatacta aatctgactc aacaatatca
     1621 gtctctaaag ttggaatttg tgtttcatat ttataagcca tcatcaagac acgaagagct
     1681 tgttttgcga gttctttatt ggtatttagg atagcctctt tatcttcagc cgtgatatca
     1741 cgaaccaagc cattatcttc aattttagta actcgtttta ataattgatc aggagcaccc
     1801 ttgactgcga taaagtagcg accatctgat tctttatgaa tcgtagacat taacttacga
     1861 tctgaatcaa atggtaactc agcgacacgt ggttcatttt tcaaaacttc acggacatca
     1921 aaattcttat ctaaaccaaa ttgtaccagt gcagtctctg ttgggtcacc gattaatttt
     1981 cctgaaggat caatcttagt atcgttggag aaattcataa tacgtaaagt gttattatca
     2041 actgaaattt cttccgaaga tgattggaga acgccattag tatacacttt ttcgacagtc
     2101 atttgattca ttgtcagagt accagtctta tccgatgcaa tgatttctgt agaacctaaa
     2161 gtttcaactg cagggagttt acgaacgata gaattacgtt tagcaagagt ttttgttccc
     2221 attgataaga caatcgtaac aattgcagga agcccttctg gaatagctgc aactgctaaa
     2281 gcaactgaag tcatcaatcc ttcaatccaa ccttctttac ggacaaatat ccccactaag
     2341 aaagtaataa ccgcaataat cacgattaag taagtcaata atttagaaag ttgaactaaa
     2401 ctttgtttca atggtgtttc agattcatca gcatttgcca acatatcagc aattttacca
     2461 acttcagtat acatccctgt gttagtaaca acaccatatc ctcgaccata cgtgacattt
     2521 gaattttgat acgccatatt cacacgatca ccaatacctg catcctctgc tactacttga
     2581 gatatgtctt tctcaacagg tactgactca ccagttagag ctgcttcttc aatttttaaa
     2641 gacgcggctt ctaaaagtcg taaatctgcg ggaacaacat ctcctgcttc taacataacg
     2701 aggtcaccag gaactagttc tttagaatcc acctcaatag tatgaccatc acgacgaaca
     2761 cgcgcaattg ggcttgacat atctttgagg gcttcaatag ctgcttcggc ttgcccctct
     2821 tgataaacac caaaagctgc atttaagata acaactgcca agataatcaa tgcatccgtt
     2881 aagccatgca taccttccgt aataacagag agagctgcag caaccagtaa gataataatc
     2941 attaaatctt tgaattggtc aaaaaactta gctattagac tacgtttttt cccttcttca
     3001 agctcattgc gaccatacat ctctaagcga tttttcgctt ctgttgttga taatccttca
     3061 cgagaagatt ctaaagacgt tagaacctct tcttgacctt gtgtgtaaaa caaggatttt
     3121 ttctgttctt ttaacaaaac ctttcctcct ttggtctctc cacaaacaaa aagagacctg
     3181 tttttatgaa aacaagtctc gctgtttaag acaacgccgg aaatttcttt cgtaatgacg
     3241 acattgccac ggtaaaccgt ctgctactcc cttgatatgt taatcattgt aacagaaaaa
     3301 aatccatttt tcaattaatt atccttgcta tcactaataa tctcaaggga aaaaacgaag
     3361 ttcatctagt cgaaataaac catactgcag tttatcaaat aaatggctct cataaagaga
     3421 tcccagcgtc ttcagtagac gaaattcata atgactactc ttttgttaat ggtaattatc
     3481 tcaataccat tgaatcttta attcataatt tgcaaaacag gctcctgtct ccgtttgctt
     3541 aaggacataa ttgatgtgca acctttgctg tgacaaatca aactgatagt gacttgtctc
     3601 tgtaatcaaa tgttgaatac caagtggagt ggggatatta accaaagcag gcgtttcacg
     3661 atgaaaacgc attacagact taggatttga aaacctcgtc ataagcaatt cctcatgatt
     3721 tgccttaata actactctct cgccctcagc attaatataa ttcaaataaa cgtaacttcc
     3781 tttttcaata aattgacaat cgtgaatctc atgaatcatt tcggtttcgt tgtcaatttg
     3841 aatgttattg ttaatttcta gtttcatgac ttctctcact tcttttatcc ttctattcta
     3901 ccaatttctg actcaagttt caatttttgc tataatatat atatgaatga aaaagtattt
     3961 cgtgaccccg ttcacactta tatccatgta aataatcaag tcatatatga cttgattaac
     4021 actaaagaat ttcaacgttt acgacgcatt aaacaaacct caacgacttc ttttaccttc
     4081 catggtgcag agcatagtcg tttttcacat tgtctcggag tttatgaact ggctcgtaaa
     4141 gttacagaaa tctttgatga acattattct gatctttgga ataaaaatga gtccctgcta
     4201 acgatggctg ccgccctact acatgatatc ggacatggcg catattctca tacatttgag
     4261 cgtctcttca atactgatca tgaagcttac actcaggaaa tcatcaccaa tcctaccaca
     4321 gagattaatg ctattttacg aaaggtggct cctgattttc ctgataaagt agctagtgtt
     4381 attaatcata gctaccccaa taaacaagtt gttcaattaa tttctagtca aattgattgt
     4441 gatcgaatgg actatcttct cagagattct tactacactg ctgctagcta tggacaattt
     4501 gatttaacac gaattttacg cgttattaga ccaaccgatt ctggaattgc ttttgctcgt
     4561 aacgggatgc acgctgtgga agattatatt gttagtcgct tccaaatgta tatgcaagtt
     4621 tatttccatc cagccagtcg tgctatggaa ttactcttac aaaatttact aaaacgtgct
     4681 cgcttcttat ttgataccca tcgcgatttt tttgaacaaa cttctccaaa tcttattcct
     4741 ttcttcacgg atcaatatga tttacaagat tatttagccc tagatgatgg ggtaatgaat
     4801 acttacttcc aatcttggat gcaagctgat gataacattt tggcagattt agctaatcgt
     4861 tttatcaatc gaaaagtctt taaatcaatt acctttgaag aggctgataa agaaaatcta
     4921 gttaaaatga aagaactagt ctcacaggtt ggttttgatc ccgattatta cactggtgtc
     4981 catgctaatt ttgacttacc ttacgatgtt tatcggcctg aacattcaaa tccacgaaca
     5041 gaaattcaaa ttatccaaaa gaatggacaa cttgctgaat tatcaagctt atcaccgatt
     5101 gtaaaagcat taactggatc taattatggt gatcaacgtt tttattttcc aaaagaaatg
     5161 ttgacattag atagcctatt ttcaagtaca aaagaagaat ttcaatctta tatcacaaat
     5221 gagcatttga cactcacgaa agataatagc cattcatgac aaacccttta ttttttagta
     5281 agatataagg tgagtatttt aggatattga aataaagagt gggagataaa atgacttcaa
     5341 ttaaacttgt tgccgtagat attgacggca ccctattaaa tagtaagcgt gaaattacac
     5401 ctgaagtggc taaagctgtt caagaagcaa aatcaaaaga cgttaaaatt gttattgcta
     5461 caggacgtcc aatcatcgga gtccaagatc ttttagaaga attgaaactc aatgaagagg
     5521 gagattatgt tatcaccttt aacggcggtc tagttcaaga cacagccaca ggagatgaca
     5581 tcatcaaaga aaccttaacc tatgaagact atctggattt cgaactgtta gcgcgtaaat
     5641 taggcgtcca catgcacgcc attacaaaag aaggaattta cacagcgaat cgtgatattg
     5701 gtaaatatac aatacacgaa gcaactttag ttaatatgcc gctcttctat cgtactcctg
     5761 aagaaatggg agacaaagaa atcattaaac taatgatgat tgatcaacct gacatcttag
     5821 atgcagcaat tgctaaaatt ccaaaaaaag ttctcgataa ctacaccatt gtaaaatcaa
     5881 ctccttttta tttagaaatt ttacccaaaa acgttaataa aggcactgca cttctccacc
     5941 ttgcagagaa aatgggatta actgtagatc aaacaatggc tattggtgat gaagaaaatg
     6001 accgtgctat gcttgaagtt gttggaaatc ctgttgttat gcaaaacggc aatcctgaac
     6061 taaaaaaaat agcaaaatat atcaccaaat caaacgagga aagtggcgta gcctacgcac
     6121 tacgtgaatg ggtaataaac tgatgtatca tgttaccgtt ggaatatccg aaaaagaata
     6181 tgatgccttt gctattgcat cgtctcaaac caatctcctc cactcttcaa agtgggcaca
     6241 agtaaaatca aattggcaaa atgaacgttt aggcttttat aaagatgacc aattagttgc
     6301 tgtagctagc atcttaatta aatcattacc tcttggtttt acaatgcttt atattccacg
     6361 tggacctatt atagactaca gcaataaaga attagttggc tttgtgttaa aaacactaaa
     6421 aaactttgga cgaaagaaac gtgctgtttt tgcaaaattc gatccagcac ttcttctacg
     6481 tcaatatcac cttaaagaag agaatgtcgc cgaagagata gatgaaagta gacaggcaat
     6541 tgataatctt aagtcagcag gtgcccaatg gataggtcca acgaaagcca tttccgaaac
     6601 catccaacca cgttttcaag cgaacatcta taccaaagca aatatagaag aaaactttcc
     6661 aaaacacact aaacgtctga taaaagatgc aaagcatcgc ggtgttcaaa tttatcgtgc
     6721 caacattgat gatttaccta aattcgccac ggttgttaac cgagaatcgt aaaggtgtag
     6781 ctttacgtaa tgaaaactat ttccatcaac taatgacaat ttatggagaa gatgcctatc
     6841 tctatttggc taaagtcaat cttcctaaaa gattagcgca atttaaagaa caacttcttc
     6901 aaatccaaag agatttatca gaaactccat cccatcaaaa aagtcgcctg acaaggctaa
     6961 atcaacaaga agcttctgtt aaacaatata tccttgaatt tcaggagttt agcaagaaat
     7021 atccagatga gcctgtcatt gcaggaattt tgtccattcg ttttggcaat gtcctggaga
     7081 tgttgtatgc tggtatggat gatagcttcc gtaaatttta tccacagtat ctcttgaatg
     7141 ctagggtgtt cgaagatgct ttcaaaaacg gtattgtatc tgctaatcta ggcggtgtcg
     7201 agggaagtct taacgatggt ttaacaaaat ttaaaagtaa cttcaatcca atgtttgaag
     7261 aatatattgg cgagtttaat ctagcaatcg atccattatt atataaattg gccaacctag
     7321 cctatactat tcgaaaaaaa caaagacact ctcactaaga aaggaaactc atgtatagag
     7381 aaattaccgc tgtcgaacac gatcgctttg tgagcgaatc caaccaaaca aacctacttc
     7441 aatcttctaa ttggcccaaa gtaaaagaca actggggtag tcaattactt ggcttttttg
     7501 acggtgaaac ccaaattgcc agcgctagta ttctcatcaa atcacttcct cttggcttct
     7561 ccatgctgta tattccgcgt ggaccaatca tggattactc caatctagat attgtaacta
     7621 aggtccttaa ggaccttaaa gcttttggca aaaaacaaag agctctcttt atcaagtgtg
     7681 atcctctcat ctatttaaaa atggtcaatg ccaaagattt tgaaaattcg cccgatgaaa
     7741 aagaagggtt aatcgccatt gatcatttac agcgtgcagg tgctgattgg actggtcgga
     7801 caacagattt agctcatact atccaaccac gttttcaagc aaacctatat gctaatcaat
     7861 ttggacttga taaaatgtct aaaaaaactc gtcaagctat tcgaacttct aaaaataagg
     7921 gagtagatat acaatttggt agccatgaac tacttgaaga ttttgcagag ttaatgaaaa
     7981 aaacagaaga ccgtaaaggg ataaatctta gaggaattga ctactaccaa aaactccttg
     8041 atacgtatcc taataactca tacatcacaa tggcttcgct agatgttgca aaacgattag
     8101 aaaaaataga aaaagaatgc cagatagctc aatctgaaag aataaaatca cttgaactta
     8161 atcgtgagaa aaaagttaaa caacaccaag atacgattga tcgattaaat aaagaaattg
     8221 attttctcaa agaagctcaa aaagcctatg accgagacat tgttccattg gctgcaaccc
     8281 tcactctaga atttggtaat acatcagaaa atatttatgc tggtatggac gattacttca
     8341 aatcttattc tgctcctatt tacacttggt ttgaaacagc tcaaagggct tttgaacgtg
     8401 gaaatatttg gcaaaatatg ggggggattg agaatgactt atctggtggt ttatatcatt
     8461 ttaagtctaa gttcgaacca atcattgagg aatttattgg agaatttaac attccagtga
     8521 acagattgct ttataaagcc tctaattatg tctatgcctt acgcaaaaaa cgtaatagct
     8581 agttaggaaa ggatactttt atggcgctta aagaattaac agctaaagag ttcgaaagtt
     8641 attcaggaaa ttacgattta caatcgttta tgcaaacccc tgagatggca aagcttctaa
     8701 aaaaacgtgg gtatgacatt acctacatgg gctaccaaat cgatgggaaa atggaaatca
     8761 tcagtattgt ttataccatc ccaatgactg gtggacttca tatggaagtc aattctggtc
     8821 cagcccattc caattctaaa tatctgaaac atttttataa agaactacag aactatgcca
     8881 aatctcaggg cgctttagaa ttattaatta aaccttatga tacctaccaa gagtttactg
     8941 gtgaaggtga acctaaaggt gcaccgaaca cctatcttat tgatggcctg actagtatag
     9001 gttatcacca cgatgggctt catattggtt atcctggcgg tgaacctgat tggcattatg
     9061 ttaaaaatct agaaggtatc actcctcaaa atcttcttaa atctttctct aaaaaaggac
     9121 gccccttggt taagaaagcc atgtcttttg gtataaaaat acgtgtcctc aagcgggaag
     9181 aactacatat ctttaaggat attacttctt ctacttctga tcgccgggac tatatggata
     9241 aatctctaga ttattatcaa gatttttatg atagtttcgg agataaggcg gaatttgtca
     9301 ttgcaactct aaactttagg gaatatgatc ataacctaca attaaatgca aagaaattgg
     9361 aagaacagat tacagtcctt gataatcgcc atcaaaataa tacagattct gccaaatacc
     9421 atcgtcaacg tactgagctt gtcaatcaac tagctagttt agataaacgt cgcaaagaag
     9481 tcgaaccttt tattcaaaaa tttggaaatc aagatgttgt tttagctggt agcctcttta
     9541 tttatagccc taaagaaact gtctatcttt tctctggctc atacacagaa tttaataaat
     9601 tttatgcgcc tgcagttctt caagaatatg ttatgcaaga ggctctaaag cgtcaatcaa
     9661 ctttctataa ttttttaggt attcaaggaa attttgatgg ttcagatggc gtacttcgct
     9721 ttaaacagaa ttttaatggt
//
LOCUS       DATROD010000069         9619 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.069, whole genome shotgun sequence.
ACCESSION   DATROD010000069 DATROD010000000
VERSION     DATROD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9619
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.069"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            79..1296
                     /locus_tag="VBC74_001442"
     CDS             79..1296
                     /locus_tag="VBC74_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6942384.1"
                     /translation="MSEYKTIHPNARLKDQYDNTIGVVSEVYDNTSHSGEQIYAVVDN
                     RHIPKEDVKEVTVLFRGSTSPQEFINNPADVTLDWLENDIPMASNIWVMKDFGNPHNF
                     SAVSPQLKASSEHLKEIMKKYPNAKINIGGHSLGGMDAQYAVVDITDKKDLKRINSVH
                     VYNAPDIYPTLTKGQKKTADSLKSKIVVYVDPNDFIGMVGREGKKGSEDSVGTVYYTE
                     SPDINWIDQHMTYGYRMENGQIKVIETNLPPEVRTTRKAMGSFYKDKKKFKKSGKGLS
                     SHEKIFLDAEQAAVIASGLVSTAETALEDIKTSAKTAVGEAEELWNTTKSIPFGITEL
                     TEAELAEAYAEGGVTYDSIVTKTDTHFDEKVKNAGDLVTTYTTLKSDIQSGVETMLSK
                     DSELAGDFKAWEN"
     gene            1284..1685
                     /locus_tag="VBC74_001443"
     CDS             1284..1685
                     /locus_tag="VBC74_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942385.1"
                     /translation="MGKLDAKDDKLSNSKKDLEKLEDDYHHKTIAISDKFFELEDKRC
                     EFETMLQETYEATSYHLRQDEMINEESFMTMNHIIEAFQSDFDTEYTKEKRRLTALEE
                     ETNQKYSKKRQLLEEKIDHLMSERRDYGNPW"
     gene            1669..1974
                     /locus_tag="VBC74_001444"
     CDS             1669..1974
                     /locus_tag="VBC74_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942386.1"
                     /translation="MGILGDYVLSEASDYISGKKDIKALKKKLDKMLVTGVYAPRIKS
                     KRRSIVSTYDAEITTTATNAKASITAIAGQIDTAIKGQFRTKVETVLDNNSTKYDEI"
     gene            complement(2163..2235)
                     /locus_tag="VBC74_001445"
     tRNA            complement(2163..2235)
                     /locus_tag="VBC74_001445"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2200..2202),aa:Lys,seq:ctt)
     gene            complement(2517..3785)
                     /locus_tag="VBC74_001446"
     CDS             complement(2517..3785)
                     /locus_tag="VBC74_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEO6942387.1"
                     /translation="MADNLALRMRPRNINEVIGQQHLVGNGKIIDRMVAANMLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGNIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKKAIQ
                     LAISDKERGFPFLVTIDDGALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDGSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPEAQIHTVTALEAAQRIGFPEARILIANIVVDLALSPKSNSAYL
                     AMDAALADLRRSGNLPIPRHLRDGHYSGSKTLGNARDYKYPHAYPEKWVKQQYLPDKL
                     VGHNYFEANETGKYERALGSNKERIDKLSD"
     gene            complement(3778..4269)
                     /locus_tag="VBC74_001447"
     CDS             complement(3778..4269)
                     /locus_tag="VBC74_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001879696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6942388.1"
                     /translation="MIIRPMQNCDREAVLTLQNTGWTALTSPVYDHKWTESDLEKNLA
                     NGMSFFVAEIDDKIAGVLDFGPYYPFPAGKHVATFGILIAEPYQGQGLGKALLKALLT
                     EAKAQGYIKIAMHVMGNNSRAISLYQKYGFTEEARITKAFFIENHYVDALIFAKDLEN
                     NHG"
     gene            4596..5066
                     /locus_tag="VBC74_001448"
     CDS             4596..5066
                     /locus_tag="VBC74_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEO6942389.1"
                     /translation="MAKFGFLSVLEEELDKHLQYDFAMDWDKKNHTVEVTFILEAQNS
                     SAIETVDDQGEMSSEDIVFEDYVLFYNPVKSRFDAEDYLVTIPYEPKKGLSREFLAYF
                     AETLNEVATEGLSDLMDFLTDDSIEEFGLSWDTDAFENGRAELKETEFYPYPRY"
     gene            5202..5660
                     /locus_tag="VBC74_001449"
     CDS             5202..5660
                     /locus_tag="VBC74_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEO6942390.1"
                     /translation="MDIFDELFDFSGAKIALFCEGKILTSLRDDFPDLPYAGFWDLPG
                     DGREGDETPLECLFREVSEELNLTLTRNHIDWVKTYRGMLNPDKLSVFMVGHISQKEY
                     DSIVLGDEGQDYKLMSIDEFLSHKKVIPQLQERLRDYLEVEDDYFTKGGS"
     gene            5632..6102
                     /locus_tag="VBC74_001450"
     CDS             5632..6102
                     /locus_tag="VBC74_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6942391.1"
                     /translation="MITLQKAEASDLEKIIAIQRASFKAVYEKYHDQYDPYVEEVEQI
                     RWKLVERPDCFYHFVLVDETIVGFLRLVIKDEEKRAWLGTAAILPQYQGQGYGSAAMA
                     LLEKTYPKLTKWDLCTIAQEKLMVSFYEKCGYHSTHTEPEQEGMDMVYMTKKIE"
     gene            complement(6141..6857)
                     /locus_tag="VBC74_001451"
     CDS             complement(6141..6857)
                     /locus_tag="VBC74_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6942392.1"
                     /translation="MKTVKEVSILSGVSVRTLHHYDKIGLFPPTALSEAGYRLYDDEA
                     LIRLQEILLFRELEFPLKDIKYLLEQAKEERQDLLAQQIKLLEWKRSHLEQVITHAKR
                     LQEKGYAYMNFDVYNKTELEQLQAEAKEKWGQTAAYQEFAQKHASDDFAQISQEMAKI
                     MVQFGQLKTQNVSDESVQMCVKRLQDYISQHFYTCTNEILAGLGQMYQSDDRFSQSID
                     KAGGAGTSEFVSQAIAYYCQ"
     gene            6981..7439
                     /locus_tag="VBC74_001452"
     CDS             6981..7439
                     /locus_tag="VBC74_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011074755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HEO6942393.1"
                     /translation="MEIVELLREWYSSFEVKEEKWNEKYEGILIRIEGDGNYKRCRLA
                     HKTASKMGYFTVFWQKDGNGKNIPFYEQDEVEDLIIVIKDGRRKGLFIIPKEVAVSKG
                     ILSSANSQGKMAMRFYSPWCSDLNRTALVTQRWQLNYFIDLSKNNEGVTT"
     gene            7436..8389
                     /locus_tag="VBC74_001453"
     CDS             7436..8389
                     /locus_tag="VBC74_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEO6942394.1"
                     /translation="MNTWNELTVHVNREAEEAVSNLLIETGSQGVAISDSADYLGQED
                     RFGELYPEVEQSDMIAITAYYPDTLDIEAVKADLADRLANFEGFGLATGSVNLDSQEL
                     VEEDWADNWKKYYEPARITHDLTIVPSWTDYEAKAGEKIIKLDPGMAFGTGTHPTTKM
                     SLFALEQVLRGGETVIDVGTGSGVLSIASSLLGAKDIYAYDLDDVAVRVAQENIDMNP
                     GTDNIHVAAGDLLKGVQQEADVIIANILADILIHLTDDAYRLVKDEGYLIMSGIISEK
                     WDMVRESAEKAGFFLETHMVQGEWNACVFKKTDDISGVIGG"
     gene            8389..9129
                     /locus_tag="VBC74_001454"
     CDS             8389..9129
                     /locus_tag="VBC74_001454"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEO6942395.1"
                     /translation="MQQYFVNGKADDFVTIEDKDTIKHMFNVMRLTEDDQVVLVFDDA
                     IKRLAKVVDSSAHRFQILEELDSNVEMPVQVTIASGFPKGDKLDFVTQKATELGAAAI
                     WGFPADWSVVKWDGKKLAKKEDKLAKIALGAAEQSKRNRLPQVRLFEKKADFQAELAG
                     FDKIFIAYEESAKEGEMSALAQNLQTVKAGDKLLFIFGPEGGISPKEIAAFEEVGAIK
                     VGLGPRIMRTETAPLYALSVISYSAELL"
     gene            9126..9619
                     /locus_tag="VBC74_001455"
                     /pseudo
     CDS             9126..9619
                     /locus_tag="VBC74_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582354.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
BASE COUNT         3039 a         1606 c         2129 g         2845 t
ORIGIN      
        1 agtgcagatt tagataatga tttagaaacc aataaaaaat gaaaaaaata tattgatgaa
       61 gaaaaattac aaatcgcaat gtcagaatat aaaacaatcc atcccaacgc cagattaaag
      121 gatcaatatg ataacacaat tggagttgtt tcggaggttt acgacaatac ttctcattct
      181 ggagaacaga tttatgctgt ggttgataat cgtcatattc caaaagaaga tgtaaaagag
      241 gtgacggtac tttttcgtgg atcaacatct cctcaggaat ttattaataa tcctgcagat
      301 gtgactttgg attggttaga aaatgacatt cctatggcaa gtaatatttg ggtaatgaaa
      361 gattttggga atcctcataa tttcagtgct gtatcaccac agttgaaagc ttcatcagaa
      421 catttaaaag agattatgaa aaaatatcca aatgcaaaaa taaacattgg tggacattca
      481 ttaggaggta tggatgctca atatgctgtt gtagatatta cagataagaa agacttgaaa
      541 cggattaact ctgtacatgt ttataatgct ccagatatat atcctacact tactaaagga
      601 cagaaaaaga cggcagattc actgaaatct aagattgttg tttatgttga tcctaatgat
      661 tttattggca tggtggggcg tgaaggaaaa aaaggaagtg aagactctgt aggaacagtc
      721 tattatacag aaagtccaga tatcaactgg atagatcaac acatgaccta tggctatcgt
      781 atggaaaatg gtcagattaa agttattgaa acgaatttac caccggaagt taggactact
      841 agaaaagcta tgggatcttt ttataaggac aagaaaaaat ttaaaaaatc tggtaagggt
      901 ctaagttcgc atgaaaaaat cttcttggat gctgaacaag ctgctgttat tgcaagtggt
      961 ttagtctcta ctgctgaaac tgctttagag gatatcaaaa catctgcgaa aacagctgtt
     1021 ggagaagcag aagaattgtg gaatacgact aaatcgattc cttttggtat tacggagctt
     1081 acagaagcag agttggcaga agcttatgca gaaggtggtg tcacttatga tagcatagta
     1141 actaaaactg atactcattt tgatgagaaa gtcaaaaatg caggagattt agtaacaaca
     1201 tatacgactt tgaaaagtga tattcaatca ggtgttgaaa ccatgttatc taaggatagt
     1261 gaattagctg gagattttaa agcatgggaa aattagacgc taaagatgat aaattaagta
     1321 acagtaaaaa ggatttagaa aagttggaag atgattacca tcataaaacc atagctattt
     1381 cagataaatt ctttgaactg gaagataaac gatgtgagtt tgagacgatg cttcaggaaa
     1441 cttacgaagc aacaagttat catcttcgtc aggatgagat gattaatgaa gagtccttta
     1501 tgaccatgaa tcacattata gaagcttttc aaagtgattt tgatacagaa tatactaaag
     1561 agaaaagaag attgactgcc ttagaagaag aaactaatca aaagtactct aaaaagagac
     1621 aattgcttga agaaaaaata gatcatttaa tgtcagaaag gagagactat gggaatcctt
     1681 ggtgattatg ttttatcaga agcctctgat tatatcagtg gaaaaaaaga tatcaaggct
     1741 ttaaaaaaga aacttgataa aatgttagtg acaggtgttt atgctccaag aatcaagtct
     1801 aaacgtagaa gtattgtctc tacctatgat gcagagataa cgactactgc aacaaacgca
     1861 aaagctagta taactgccat tgcaggtcaa atagacactg caataaaggg acagtttaga
     1921 actaaagttg aaactgtatt ggataataac agtacaaagt atgatgaaat ttaaaagaca
     1981 tgggctaaat aatttagctc tttttttata ctcaaaaata agagaaatac aaaacaaaat
     2041 acatatttat gcaagtaaag agaaataaaa aaatcaccat tttatctgtc aaaaaaaatt
     2101 ggtgggcaag tgtaaaaaga gcgtcaaaaa agcctatagg tataggcttt gttttataag
     2161 actgccccct acagggctcg aacctgtgac ccatagatta agagtctact gctctaccaa
     2221 ctgagctaag gaggctagga agctgtattg gcaccgatga tccacttttt gtattgatcc
     2281 cgcacaaaat aagcaggtgg gtaactcgca cctctttaag tggcttccgc gtgttaacgg
     2341 cttgcgcact agagatgact tcgtttcccg aaatacaaag aaatagtcgg tcaacactta
     2401 gatgtggatt cgtatgccac agcaatgttt ctatgtcttt atgataccac tttttataaa
     2461 attttcaagc aaaattatcg atttttgaaa tcgattgcga tttttaaaac tggcaatcag
     2521 tctgagagct tatcaatgcg ctctttattt gaaccaagtg cacgttcgta tttaccagtt
     2581 tcattggctt caaaataatt atgtcctact agtttatctg gtaagtattg ttgtttaacc
     2641 catttttcag gataggcgtg agggtacttg taatcccttg cattgcccaa ggttttacta
     2701 ccgctgtaat gtccatctct taggtgacgt ggaataggta aatttcctga acgacgtaaa
     2761 tcagctaagg ctgcatccat agccaaatag gctgaatttg atttaggtga tagagctaaa
     2821 tccacaacaa tgttagcaat aagaatacga gcttctggaa aaccaatgcg ttgggcagct
     2881 tccaatgcag tcacagtatg tatttgtgct tcggggttag ctaagccgat atcttcataa
     2941 gcaataatgg tcaaacgtct tgctaaacta ggtaaatctc cagcttctac taaacgagcg
     3001 gcatagtgca ggctggcatt gacatcagaa cctctaattg atttttgtaa ggctgatagg
     3061 acatcataat gcccatctcc gtttttatcc attgtaatat aactacgttg cagactattt
     3121 tccattgtct ctagactaat atgacggcta ccatcttcgt taggagaagt agacatgact
     3181 gctagatcca aggaattata agcagatcgt aggtctccat ttgtggcggt aacaatgaaa
     3241 tctagcgccc catcatcaat tgtcactaaa aaaggaaatc cccgttcttt atcggaaatt
     3301 gctagttgaa tggctttttt aatatcctcg ttagaaagag gttctagctc aaaaatttgt
     3361 accctacttc taatggcagg agttactgaa aagaaaggat tttcagttgt tgctccaatc
     3421 ataataatat tgccattctc aagtaaaggt aaaagaaaat cttgttttgt cttgtcaagt
     3481 ctatgaatct cgtcaagtaa taaaactaag ccaccagaaa atttagcttc ttctgcaatt
     3541 tcttgaagac gttttttact atcaacagtg gcattaaaag tacgaaaggc atatttagta
     3601 gtaccagcaa tagcagaagc aatagaagtc ttcccaatac ctggtggtcc atataggatc
     3661 atggaagata acatattagc tgcaaccatt cgatcaataa ttttgccatt accaacgaga
     3721 tgttgctgcc caatgacttc attaatattg cgaggacgca tacgtagagc aagattgtca
     3781 gccatgatta ttctccaaat cttttgcgaa aattaaagca tcaacataat gattttcaat
     3841 gaaaaaggct ttagtgattc gagcctcttc ggtaaaaccg tatttttgat agagggaaat
     3901 ggctctacta ttgttgccca tgacgtgcat ggcaatcttg atgtaacctt gagctttagc
     3961 ctcagttagc agagctttca agagagcctt acctagcccc tgaccttggt agggttcagc
     4021 gatgagaatg ccgaaagtgg caacatgctt gccagctggg aaaggatagt agggaccaaa
     4081 gtccaagacg ccagcgatct tatcatcgat ctcagcgaca aagaaggaca tgccatttgc
     4141 cagattcttc tccaagtcac tctcagtcca cttgtggtca tagacaggag aggtaagagc
     4201 agtccagcca gtattttgta aggtcagaac tgcctcacgg tcgcagttct gcataggtct
     4261 gataatcata attgatgata caaagggacg gttcggttta cgaaaatttt cgtaaagaaa
     4321 tagggaacga cgaagtgtcg ttagacacta ggaagttcat cttttctttt agaaaattag
     4381 tcccgtgttc aaatcctttc tattttttat atagagtttt tctctcagct gactctttta
     4441 acatagtatg aagtcagtca aaaacgacta gtctttgaga ctattgtaac ataaaaataa
     4501 gtaggaacta ccttttgatt aacctgagtc tgtaacgttt ttcttctata ttcatgttat
     4561 aataagaaag aataaataga aaataaggac ataagatggc taaatttgga tttttatcgg
     4621 tactagaaga agaattggat aagcatttac agtatgactt tgccatggat tgggataaga
     4681 aaaatcatac tgtagaagtc acttttatat tagaagcaca aaacagctct gctattgaaa
     4741 ccgtggacga ccagggggag atgagtagcg aggacatcgt ttttgaggac tatgtactct
     4801 tttacaaccc tgtcaagtct cgctttgatg ctgaggacta tctggtgact atcccctatg
     4861 agcctaagaa gggcttgtcg cgtgagtttc tggcttattt tgcggagact ctcaacgagg
     4921 ttgccactga agggttgagc gacctcatgg acttcctcac cgatgacagt atcgaagagt
     4981 tcggtctttc ttgggatacg gatgcttttg aaaatggcag agcagaactg aaagagacag
     5041 agttttaccc ttatccgaga tattagactt gatggatttg ctaaaatgtg gatatacatt
     5101 ggatgatata gaaacagcca gaccagagga tatggaacgt tattatcccc ctgaacaaat
     5161 cagaaaatat ggagctttag gaatagaatt gaggttattg catggatatt tttgatgagt
     5221 tatttgattt ctcaggtgca aagatcgctc ttttctgcga gggaaagatt ttgaccagcc
     5281 taagagatga ttttccagat ttgccctatg caggcttttg ggacttacca ggcgatggtc
     5341 gtgagggcga tgaaacacct ttggaatgtc tttttcgaga agttagtgaa gaattaaatc
     5401 tgaccttaac tcgaaaccat attgattggg tcaagactta taggggtatg ctgaaccctg
     5461 ataaattatc ggtttttatg gttggacata tcagtcagaa agagtacgat agcattgttc
     5521 taggcgatga aggacaagac tacaagctca tgtccattga cgaattttta agccataaaa
     5581 aggtcattcc tcagttgcag gagaggttga gagattattt ggaggtggaa gatgattact
     5641 ttacaaaagg cggaagctag tgatttagag aagattattg ccattcagag agctagcttt
     5701 aaggcagtct atgagaaata ccacgatcaa tacgatccct atgtggaaga agttgagcag
     5761 attcgatgga aactagtgga gcgaccagat tgtttctatc attttgtcct tgttgatgag
     5821 acaattgttg gttttcttag attggttatc aaggacgagg agaagcgtgc ttggcttggt
     5881 acggcggcta ttcttcccca atatcaagga cagggctacg gctcagcagc gatggcttta
     5941 ttagagaaaa cctatcctaa gcttacaaag tgggatttat gtacgattgc gcaagaaaaa
     6001 ctaatggtca gtttttatga aaaatgtggt tatcattcaa cccataccga acctgagcaa
     6061 gaaggaatgg atatggtcta tatgacaaag aagatagaat aaaaaaagaa aactagatga
     6121 ttgtctagtt ttctgatagt ttattggcaa tagtaagcaa tagcttggct gacaaattca
     6181 cttgttcctg ctccaccagc tttgtcaatt gattgactaa accggtcatc tgattgatac
     6241 atttgtccta gtccagcaag gatttcattg gtacaggtgt aaaaatgctg agagatatag
     6301 tcttgtagcc gtttcacaca catttgaaca gattcatcag atacattttg tgtctttaat
     6361 tgcccaaatt gtaccattat ttttgccatt tcttggctaa tttgtgcaaa gtcatctgaa
     6421 gcatgttttt gagcaaattc ttggtaggct gctgtttgtc cccatttttc ttttgcttca
     6481 gcttgtaatt gttctaattc tgttttatta taaacatcga aattcatata agcatatcct
     6541 ttttcttgta atcgtttcgc atgggtgata acctgctcaa gatgcgatct tttccattca
     6601 agcaatttga tttgttgggc taataagtct tgcctttcct cttttgcttg ctcaagcagg
     6661 tatttgatat ctttgagagg gaattccaac tcacgaaata gcaaaatttc ttgcaggcga
     6721 atcaatgctt catcgtcata aagtcgatag cctgcttcgg atagggcggt aggtgggaat
     6781 aaaccgattt tatcatagtg atgtaaggtg cgcacactta caccgcttaa gatactgact
     6841 tctttaactg ttttcatggt aataacctcc tctcacttac atagtataaa ggataaccta
     6901 aggtcagggt caagcaaaaa ataaattatt ttgaaaagta tggttaatca tattgatttg
     6961 aagataaagg agggatagtt atagaaatag ttgagctact aagggaatgg tattcttcct
     7021 ttgaagttaa agaagaaaag tggaatgaaa aatatgaagg tatcctcata agaattgaag
     7081 gcgatggaaa ctataaaagg tgtcgtttag ctcataagac agctagcaag atggggtatt
     7141 ttacagtatt ttggcagaaa gatggaaatg gtaaaaatat tcctttttat gaacaagatg
     7201 aagtggaaga tttgattatt gtcattaaag atggtaggag gaaaggtttg ttcatcattc
     7261 ccaaagaggt tgcagtgagt aagggtattc tttcatctgc taactctcaa ggaaagatgg
     7321 ctatgcgatt ttactctcct tggtgtagcg atttaaatag aacagctttg gtgacacagc
     7381 gatggcagtt gaactatttt attgatttaa gtaaaaataa tgaaggagtt actacatgaa
     7441 cacatggaat gaactaacgg ttcacgtgaa tcgtgaggca gaggaagctg tctcgaattt
     7501 attgattgaa actggcagtc agggggttgc cattagcgac tcggctgatt atcttggaca
     7561 ggaggatcgt ttcggcgagc tttatcctga ggtggagcaa tctgatatga tcgctattac
     7621 tgcctattat cctgatacgc ttgatattga ggcggttaag gctgatctgg ctgatcgttt
     7681 ggctaatttt gaaggctttg gtcttgcgac gggtagcgtc aaccttgaca gtcaggaatt
     7741 ggttgaagag gactgggctg ataactggaa gaagtactat gagccagctc gcattaccca
     7801 tgatttgacc atcgtgccgt catggacgga ctacgaggct aaggctggtg agaagattat
     7861 caagctggat cctggtatgg cttttgggac tgggactcac ccaacgacta agatgagtct
     7921 ttttgccttg gagcaagtgc ttcgtggtgg tgagacggtt atcgatgtgg ggactggttc
     7981 tggcgttttg tctattgcta gctctctctt gggtgccaag gacatctatg cttatgattt
     8041 ggatgatgtt gcggtgcgcg tggctcagga aaatatcgat atgaaccctg gtactgataa
     8101 tattcatgtg gctgctggtg acctgctcaa aggtgtccag caagaggcag atgtcattat
     8161 tgccaatatt ttggcggata ttttgatcca tctgacagac gatgcctacc gtttggtcaa
     8221 ggatgagggt tatctcatca tgtctggtat catttctgaa aaatgggaca tggtgcgtga
     8281 gagtgctgaa aaagctggtt tcttccttga gactcacatg gttcaaggtg agtggaatgc
     8341 ctgtgttttc aaaaagacag atgatatttc aggagtgatt ggcggctaat gcaacagtat
     8401 tttgtaaatg gcaaagcaga tgactttgtg accatcgagg acaaggatac catcaagcac
     8461 atgttcaatg tcatgcgatt gacagaagat gaccaagtgg tcttggtttt tgatgatgcc
     8521 atcaagcgtc tggcaaaggt ggtggacagc tctgcccatc gcttccaaat tctagaagag
     8581 ctagacagca atgttgaaat gcctgtccaa gtgaccatcg cctcaggttt tcccaaggga
     8641 gacaagttgg attttgtgac gcaaaaggca acagaattgg gtgctgcggc tatctgggga
     8701 ttcccagctg actggtcagt cgtcaagtgg gacggcaaga aactagctaa aaaagaagat
     8761 aagctggcaa aaattgctct gggcgcagcg gagcagagca agcgtaatcg tcttcctcag
     8821 gttcgtttat ttgagaagaa ggctgatttt caagcggagc ttgcaggctt tgataagatt
     8881 tttatcgcct atgaagagtc tgccaaggag ggtgaaatgt cagcgttagc ccagaatttg
     8941 cagactgtaa aagcaggtga caagctcctc ttcatctttg gaccagaagg gggcatttct
     9001 ccaaaagaaa tcgcagcttt tgaagaggtt ggagctatca aggtgggcct gggtccacgc
     9061 atcatgcgca cagaaacagc accgctctat gctctaagtg ttattagtta ctcagcggag
     9121 ttattatgat tattgctatt caaggtgata tggctgtagg gaaaaccaca gctttgaaaa
     9181 tcattgagaa agtttacccg aaaatcgcta ttagttttga ggatatttca ccagtagttg
     9241 ataagattaa taaacatcag cttgataaca ataaattaga cgattattgt gaaattcaac
     9301 aactatttat tcagcatgag cgagatagat acgaacttgt tagagataat gaaatagtgt
     9361 taatggatta tggagctgag gaaattgctt tctatacaca atactttcca aaggtggcgg
     9421 gttatagttg actttacctt ctaatttgtt actagaggta aataggctaa aggaatgttt
     9481 tgctgattat attatttttc tagatgcaga tgagacggtc ctataccaac gtcaaaaaaa
     9541 tgattttaat agacggcgtc attttttcaa atggtatact catcacctgt tgcctgctaa
     9601 aaaacattat ttagacaca
//
LOCUS       DATROD010000070         9606 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.070, whole genome shotgun sequence.
ACCESSION   DATROD010000070 DATROD010000000
VERSION     DATROD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9606
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.070"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..805)
                     /locus_tag="VBC74_001456"
     CDS             complement(<1..805)
                     /locus_tag="VBC74_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3744 domain-containing protein"
                     /protein_id="HEO6942396.1"
                     /translation="MKDFIEWKDFTFQYDVQSEPTLKGINLSIPKGEKVLILGPSGSG
                     KSTLGHCLNGIIPNTHKGQYSGIFTINHKNAFDLSIYDKSHLVSTVLQDPDGQFIGLT
                     VAEDIAFALENDVVAQEEMASIVEMWAKRLEIAPLLSKRPQDLSGGQKQRVSLAGVLV
                     DDSPILLFDEPLANLDPQSGQDIMALVDRIHQEQDATTIIIEHRLEDVLYERVDRVVL
                     FSDGQIIYNGEPDQLLKTNFLSEYGIREPLYISALKNLGYDFEKQNTMTS"
     gene            complement(822..1370)
                     /locus_tag="VBC74_001457"
     CDS             complement(822..1370)
                     /locus_tag="VBC74_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEO6942397.1"
                     /translation="MNTNTIKKVVATGIGAALFIIIGMLVNIPTPIPNTNIQLQYAVL
                     ALFAVIYGPGVGFFTGFIGHALKDSIQYGSPWWTWVLVSGLLGLMIGFFAKKLAIQLS
                     GMIKKDLLLFNVVQVIANLIGWSVVAPYGDIFFYSEPASKVFAQGFLSSLVNSITIGV
                     GGTLLLLAYAKSRPQKGSLSKD"
     gene            complement(1539..2747)
                     /gene="sstT"
                     /locus_tag="VBC74_001458"
     CDS             complement(1539..2747)
                     /gene="sstT"
                     /locus_tag="VBC74_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEO6942398.1"
                     /translation="MKRFLKAWRKTSLIKKITIGVVIGLFLGILVPKASAIGLLGQLF
                     VGGLKAIAPLLVFTLVISALSQHREGGKTNMSTIIGLYITATFAAALIAVVVNYIFPL
                     TLILKTPAKTDLLPPKGISEVFQSLLLKIVDNPIHAITEANYMSILFWAVIFGLAMRS
                     SNQRTKDLMQTFADATSQVVKWIINLAPIGIMGLVFTSISENGIGILGDYGLLILVLV
                     GTMLFVALVVNPIIAFVMMRKNPYPLVLRCLKDSGITAFFTRSSAANIPVNMRLCEDL
                     GLDKDTYSVSIPLGAAINMAGAAITINILTLAAVNTLGITVDFPTAFLLSVVAAVSAC
                     GASGVTGGSLLLIPVACSLFGISNDVAMQVVGVGFIVGVIQDSCETALNSSTDVLFTA
                     VAEKSVFGKK"
     gene            2923..4290
                     /gene="brnQ"
                     /locus_tag="VBC74_001459"
     CDS             2923..4290
                     /gene="brnQ"
                     /locus_tag="VBC74_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEO6942399.1"
                     /translation="MKLKNTYIIIGLMLFALFFGAGNLIYPAYLGIYSGSNWAWAIIG
                     FCLTGVTLPLLGVAAVTYSGATDVESFTKPVSKTYAIFFAVALYLSIGPFFAIPRTGA
                     TSFSVGIQPILGDSSLVKIIYGLIFFGISYFLAIRPSQIVDRIGKYLTPFLLTVISIL
                     IIASLIHPAAIVGTPHNASTNVSDAFKDLPFLAGLIQGYGTMDALASIAFAIIVIQAS
                     KQYGAITKKEITSMTLKSGAIATFLLAFIYIFVGRIGATSQSLFKFANGSFLLHNTPI
                     DGGHVLSQSANFYLGIVGQAILGTAIFLACLTTATGLITACAEYFHKLLPKISHITWA
                     TIFTLIAITFYFGGLSEIIRWSLPVLYLLYPLTIVLIFLVFFDQKFESSRIVYQTSIA
                     ATAVAALYDALSKLGEMTGLFTIPSALTTFFTKVVPLGEYSMGWISFAICGVLVGLIL
                     KKVKA"
     gene            4404..5459
                     /locus_tag="VBC74_001460"
     CDS             4404..5459
                     /locus_tag="VBC74_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEO6942400.1"
                     /translation="MRAATYLSANELSLTDKAKPQVIKPTDAVVRLVKTTICGTDLHI
                     LGGDVPACKPGTTLGHEGIGIVEEVGSAVNHFKVGDKVIISCVTACNTCYYCKHQLPS
                     HCEDGGWILGHLIEGTQAEYVHIPHADGSLYHAPEGVCDDALVMLSDILPTSYEIGVL
                     PSHIKPGDTVCIVGAGPIGLSALLTAQFYSPAKIIMVDLSQKRLEASKKFGATHTILS
                     TSTQEVKEEIDKITKGRGVDVVLECVGYPATFDICQNVVSIGGHIANVGVHGKPVEFN
                     LQDLWIKNITLNTGLVNANTTEMLLEVLETGKIDATQLVTHHFKLSEIEEAYKVFKAA
                     EENNTLKVIIENDITPA"
     gene            complement(5508..6200)
                     /locus_tag="VBC74_001461"
     CDS             complement(5508..6200)
                     /locus_tag="VBC74_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000574038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6942401.1"
                     /translation="MIEWIQTHLPNVYQMGWEGAYGWQTAIVQTLYMTFWSFLIGGLM
                     GLLGGLFLVLTSPRGVIANKLVFGVLDKVVSVFRALPFIILLALIAPVTRVIVGTTLG
                     SPAALVPLSLAVFPFFARQVQVVLAELDGGVIEAAQASGGTLWDIIVVYLREGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGSVAITKGYNYSRDDITLVATILILLLIFFIQF
                     LGDFLTRRLSHK"
     gene            complement(6197..7267)
                     /locus_tag="VBC74_001462"
     CDS             complement(6197..7267)
                     /locus_tag="VBC74_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEO6942402.1"
                     /translation="MSKEPIIKLDNIDVTFHQKKREINAVKDVTIHINQGDIYGIVGY
                     SGAGKSTLVRVINLLQEPSAGKITIDDQVIYDNKVTLTSTQLREQRREIGMIFQHFNL
                     MSQLTAEQNVAFALKHSGLSKEAKAAKVAKLLELVGLSDRAQNYPSQLSGGQKQRVAI
                     ARALANDPKILISDESTSALDPKTTKQILALLQDLNKKLGLTIVLITHEMQIVKDIAN
                     RVAVMQNGKLIEEGSVLDIFSHPRESLTQDFIKIATGIDEAMLKIEQQEVVKNLPVGS
                     KLVQLKYAGHSTDEPLLNQIYKEFEVTANILYGNIEILDGIPVGEMVVILSGDEEKLR
                     QACQAITDSQVQLTLLKEGGKA"
     gene            complement(7260..8636)
                     /locus_tag="VBC74_001463"
     CDS             complement(7260..8636)
                     /locus_tag="VBC74_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEO6942403.1"
                     /translation="MTLTNEENQIQKFLSDTIVQDDINILRDLIAIKSIFAQKVGLND
                     LSSYLGEVFRKAGAEVIIDDSYSAPFIVANFKSSKVDAKRIIFYNHYDTVPADEVEQW
                     TEDPFTLSLRYGKMYGRGVDDDKGHITARLSAVKKYLSRHKGELPLDITFIVEGAEES
                     ASVGLDYYLEKYQEQLQGADLIVWEDGPKNPKGQLEIAGGNKGIVTFDLSVSSADVDI
                     HSSFGGVVDSSTWYLIQALNTLRDNKGHILVEGIYDKVIPPTKRELELVEKYSYRSAK
                     ALEGAYQLVLPSLADSHKTFLRKLYFEPSIAIEGITSGYQGEGVKTILPAYAKCKAEV
                     RLVPGLTPKGVLDSIQNHLKENGFKDIELTYTLGEMSYRSDMSAPSILKVVDLAEQFY
                     PEGISLLPTSPGTGPMYLVHQALRAPIAAIGIGHANSRDHGVDENVSIADYYTHIELV
                     EALIESYE"
     gene            complement(8770..9594)
                     /locus_tag="VBC74_001464"
     CDS             complement(8770..9594)
                     /locus_tag="VBC74_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO6942404.1"
                     /translation="MKLKHIVLGLALTTLLGVTFSNQEVSASSTSSKVVKVGVMTFSD
                     TEKARWDKIEKLVGDKAKIKFTEFTDYTQPNQATANKDVDINAFQHYNFLENWNKENK
                     KNLIPLEKTYLAPIRIYSEKVKSLKKLKKGATIAIPNDATNGSRALYVLQSAGLIKLN
                     VSGKKVATVANITSNKKDINIQELDASQTPRALKDVDAAIINNTYIEQANLKPSDAIF
                     VEKSDKNSKQWINIIAGRKNWKKQKNAKAIQAILDAYHTDEVKKVIKDTSADIPQW"
BASE COUNT         3056 a         1895 c         1541 g         3114 t
ORIGIN      
        1 tagacgtcat tgtattttgt ttttcaaagt catatcctaa atttttgaga gcagaaatat
       61 acagtggttc tcgaatacca tattcagata aaaaattagt ttttaaaagt tgatccggct
      121 ccccattata aataatttgt ccatcactaa aaagaactac acgatctact cgctcataaa
      181 gaacatcttc caatcgatgt tcaataataa tagtggtcgc atcttgctct tgatgaatac
      241 ggtcaaccaa tgccatgata tcttgtcccg attgtggatc tagattagcc aaaggctcat
      301 caaataatag aattgggcta tcgtctacta aaactccagc taaactaacc cgttgttttt
      361 gtcctcccga caagtcttgt ggacgtttag acaaaagagg agctatttct aaacgttttg
      421 cccacatttc aacgatagag gccatttctt cctgtgctac aacatcattt tctaatgcaa
      481 aagcaatatc ttctgctact gtcaatccta taaattggcc atctggatct tgtaaaaccg
      541 tagaaactaa atgagacttg tcatatattg acaagtcaaa agcattttta tggttaatcg
      601 taaaaatacc actatattgg cctttatgag tatttgggat aataccattt aaacaatgac
      661 caagcgttga cttccccgac cctgaaggtc ctaatatcaa gaccttttca cctttaggaa
      721 tgcttaagtt aatccctttt aaagttggtt cagattgaac gtcatattga aaggtaaaat
      781 ccttccattc aataaaatct ttcatagatt actctcttat cctaatcttt ggataaacta
      841 cctttttgtg gtcgtgattt agcgtatgct agcaacaaaa gcgtaccacc tactccaata
      901 gtaatggaat tcacaaggct tgataagaaa ccttgtgcaa aaactttgct tgcaggttcg
      961 ctataaaaga aaatatcacc atacggtgca acgacagacc aacctataag gttagcaata
     1021 acttgaacta cattaaatag aagcaaatcc ttcttaatca tgccagataa ttgaatagct
     1081 aattttttag caaaaaagcc tatcatcaaa cctaaaagac cactaactaa aacccaagtc
     1141 caccacggag aaccatattg aatcgaatct ttcaatgcgt gtccgataaa accagtaaaa
     1201 aaaccaactc ctggtccata aataactgca aataatgcta aaactgcata ctgcaattga
     1261 atattagtat ttggaattgg tgttggtata ttaactagca tacctataat gataaaaagt
     1321 gcagctccaa ttccagtcgc tacaaccttt tttattgtgt ttgtattcat aaaaacctct
     1381 ctgaaaaaca agcttaaaca gtcacttaca atctgtttta ttttatcata tttcaagctg
     1441 atttgaaacc ttattgacaa gaagaactga aaaacgattg aaaataatat taaataccaa
     1501 aaaagatctc agatagaata cctaagacct tcattagttt atttttttcc aaaaactgat
     1561 ttttcagcta cagccgtgaa cagaacgtct gtcgatgaat ttaaagctgt ctcacacgaa
     1621 tcctgtatca cccctacaat gaaaccaact cctacgactt gcattgcaac gtcatttgaa
     1681 ataccaaata agctacatgc caccggaatc aataaaagtg aaccaccagt tacaccagaa
     1741 gctccacatg ctgatacagc agcaaccaca cttaataaga atgctgttgg aaaatcaaca
     1801 gtaattccta aagtatttac tgctgctaaa gtcaggatat taattgtaat agctgcacca
     1861 gccatattga tagctgcacc aagcgggata gatactgagt aagtgtcttt atctaatcct
     1921 aaatcctcac atagtctcat attaacagga atattcgccg cagaactacg tgtaaagaaa
     1981 gctgtaatac ctgaatcctt taagcatcgt aataccaaag ggtaaggatt tttacgcatc
     2041 ataacaaaag caatgattgg gttaacgaca agagctacaa ataacatcgt acccaccaaa
     2101 actaatatta aaagaccata atcacctagt ataccaatac cgttttctga aatgctagtg
     2161 aaaaccaacc ccataatacc aattggggcc aaattgataa tccatttgac aacttgtgaa
     2221 gtagcatcag caaaagtttg cattaaatct tttgtacgct gattactgct tcgcatcgcc
     2281 aaaccaaaaa taaccgccca aaatagaata ctcatataat tggcctcggt aatggcatga
     2341 atgggattat caacaatttt caacaaaaga gattgaaaga cttctgaaat ccccttggga
     2401 ggtaataagt ctgtctttgc tggcgttttc aatatcagtg tcaaagggaa aatatagttc
     2461 acaacaacag cgattagggc cgctgcaaaa gttgcagtga tatacaaacc aattatcgtt
     2521 gacatattgg tcttaccgcc ctcacggtgt tgcgacaagg cactaataac taaagtgaaa
     2581 actaaaagag gggcaatagc tttcaaccct cctacaaaaa gttgaccaag taaaccaatc
     2641 gcagaggctt tagggactaa aatacccaag aataagccaa taacgactcc aattgttatt
     2701 ttttttatca agcttgtttt tctccaagct tttaaaaatc gtttcatact aaactcctaa
     2761 aaaattctta aagtctatga tatgataaaa aaaaagattc gtcaaatacc ttttttattt
     2821 tttagaggtt gacgaattct aaatttttct gatataatta ctttttgaaa tattcaatca
     2881 atttcgaaaa taaaatattt ttttgtacag aaaggtacat tgatgaaact aaaaaacact
     2941 tatatcatta tcggcttgat gctttttgcg ctgttctttg gagccggtaa tcttatttat
     3001 ccagcctact taggtatcta ctctggttct aattgggcgt gggctatcat tgggttctgc
     3061 ttaacaggcg taactttacc cctacttggt gttgcagctg tgacgtactc aggagcaacc
     3121 gatgttgaaa gcttcaccaa acctgtctct aaaacatatg ctattttctt tgcagtagca
     3181 ctatacctat ctattggacc tttctttgcc attcctcgaa caggggcaac ctcattctca
     3241 gttggtattc aacctatctt aggcgacagc tccttagtca aaattatcta tggtttaatt
     3301 ttctttggta tttcttattt cttagctatt agacctagtc agattgtaga tcgtattggt
     3361 aaatatctaa ctcccttttt actaactgtt attagtattt taattattgc ctcactcatc
     3421 catccagctg ctatcgttgg aacaccacat aacgcttcaa cgaatgtcag cgatgctttc
     3481 aaggatttac cattcctagc aggactgatt caggggtatg gtacaatgga tgcccttgca
     3541 tcaattgctt ttgctattat cgttattcaa gcaagtaaac agtacggagc aattaccaaa
     3601 aaagaaatta cttctatgac acttaaatct ggtgctatcg ctacatttct tttagctttt
     3661 atttatattt ttgtaggacg tattggtgca acttcacaaa gcttgtttaa atttgcaaat
     3721 ggttccttct tacttcacaa tactccaata gacggtgggc atgttcttag tcaatcagct
     3781 aacttctatt taggaattgt gggacaagct attttaggta cggctatttt tcttgcttgc
     3841 ttaacaacag ctactggatt aatcactgct tgtgcggaat acttccacaa attacttcca
     3901 aagattagtc acattacttg ggctacaatc tttactttaa ttgctattac gttctatttt
     3961 ggtggtttat cagagattat ccgttggtca ctaccagttc tctaccttct ctatccatta
     4021 actatagtct taattttctt agtctttttt gatcaaaaat ttgaaagtag ccgcattgtc
     4081 taccaaactt ctattgccgc cactgctgtc gctgctttat acgatgcctt atcaaaactc
     4141 ggcgaaatga ctggtttatt taccattcca tccgctttga ccacattctt tactaaagtt
     4201 gttcctttag gtgaatattc tatgggatgg attagttttg caatttgtgg tgtcttagtt
     4261 ggtcttattc taaaaaaagt aaaagcataa taatttaaag cagtacgaaa attactgctt
     4321 ttattttaaa ataattattg taaccgcttt cttttggtgg tataatagcc ttgaaaataa
     4381 ataatttttg gaggaactct aatatgagag ctgcaactta tctttcagct aatgaattat
     4441 cacttactga taaggctaaa cctcaagtaa taaaacccac agatgcggtt gtaagacttg
     4501 tcaaaacaac catttgtggt actgatctac atatccttgg tggcgatgtt cctgcttgta
     4561 aaccaggaac aactttaggt catgaaggca ttggtatcgt cgaagaagtt ggttctgctg
     4621 tcaatcattt taaagtcgga gataaagtaa ttatttcttg tgtcactgcc tgtaatactt
     4681 gctattactg taagcaccag ttgccttcac attgtgaaga tggcgggtgg attttaggac
     4741 atcttattga aggtactcaa gcagaatatg ttcatattcc acatgcagat ggtagccttt
     4801 atcatgcacc tgaaggtgtt tgtgacgatg cattggttat gttatctgat attttaccaa
     4861 cttcatacga aatcggcgtc ttaccttctc acattaaacc aggtgatact gtttgtattg
     4921 taggcgctgg accaattgga ctatctgcct tactaactgc acaattctat tctcctgcca
     4981 aaattattat ggttgattta tctcaaaaac gacttgaagc ctctaaaaaa tttggtgcaa
     5041 cacacaccat tctatcaacg tctactcaag aagttaaaga agaaattgat aaaattacca
     5101 aaggccgtgg cgttgatgtc gtattagaat gtgttggtta tcctgcaaca tttgatattt
     5161 gtcaaaatgt agtttctatt ggaggacata tcgcaaatgt tggtgttcat ggtaaacctg
     5221 ttgaatttaa cctccaagat ttatggataa aaaatataac ccttaatact ggattagtta
     5281 atgctaatac aactgaaatg ctccttgaag ttcttgagac aggcaaaatc gatgcaacac
     5341 aactagtaac acatcacttc aagctatctg aaatcgaaga agcttataag gtatttaaag
     5401 ctgctgaaga aaacaatact ctcaaagtta tcatcgaaaa cgatattact ccagcctgat
     5461 aatttttaaa caaatgaaaa tgtgagaaga atagccttct cacattttta tttatgactc
     5521 aagcgacgtg ttaaaaaatc acctaaaaat tggataaaga aaattaataa taaaatcaga
     5581 atagtcgcta ctaaagtaat atcatcacga gaatagttat atcctttagt aatagcaaca
     5641 gaacccaatc ctcctgcacc aatagcgcca gccatagctg tttcacctac taaagaaatc
     5701 aaagtaaccg ttgatactcg aattaaatct ggtagacctt cacgaagata aactacaata
     5761 atatcccaaa gtgttccacc tgaggcttgt gcagcctcaa taactccacc atcaagttca
     5821 gctaaaacaa cttgaacttg acgagcaaaa aatgggaaaa ctgccaaaga aagaggtacc
     5881 aaagctgctg gtgaaccaag tgttgttcct acaattacgc gagttactgg cgcaatcaaa
     5941 gcaagaagaa taatgaaggg cagagctcta aaaacagaaa caactttatc taaaactcca
     6001 aatactaatt tattagcaat aactcctcta ggactagtta aaacaaggaa taaacctcct
     6061 aacaatccca ttaaacctcc aataaggaac gaccaaaaag tcatataaag ggtttgtaca
     6121 atagctgtct gccagccgta agcaccttcc caacccattt gatatacatt tggtaaatgt
     6181 gtttgaatcc actctatcat gctttaccac cttctttcaa taacgttaat tgaacttgcg
     6241 aatctgtaat tgcttggcag gcttgtctta atttttcttc gtcacctgat aagataacta
     6301 ccatttctcc aactggaata ccatctaaaa tttcaatatt cccatagagg atgttagcag
     6361 ttacttcaaa ttctttataa atttgattta aaagtggctc atccgtgcta tgcccagcgt
     6421 atttcagttg aactaatttg ctacctactg gtagattttt gacaacttct tgttgctcaa
     6481 tcttaagcat tgcctcatca atcccagtag ctatttttat aaaatcttgt gtaagactct
     6541 ctctaggatg tgaaaaaatg tctaaaactg atccttcttc aatcaattta ccattttgca
     6601 taacagcaac gcgattagca atgtctttga caatttgcat ctcatgtgta atcaaaacaa
     6661 tcgtaagtcc caatttttta ttcagatctt ggagaagggc aagtatttgt tttgttgttt
     6721 tagggtcaag tgcagaggta gactcatctg aaattaagat tttaggatcg ttagccaatg
     6781 cacgcgcaat ggcaacacgc tgtttttgcc ctcctgataa ttgtgaagga tagttttgag
     6841 cacgatcgga taatccaaca agttctaata atttagcaac cttagctgct ttcgcctctt
     6901 ttgataaacc tgaatgttta agagcaaaag ctacattctg ttctgctgtc aactgtgaca
     6961 ttaggttaaa atgttgaaaa atcattccaa tttcacggcg ctgttcgcgt aattgtgtgc
     7021 tagtcagtgt caccttgttg tcgtaaataa cttggtcatc aatagtaatt ttacctgctg
     7081 aaggttcttg tagcaaatta ataacacgaa caagggtaga ttttccggct cctgaataac
     7141 cgacaattcc atatatatca ccttgattaa tatggatagt aacatcttta acagcattaa
     7201 tctcacgttt cttttggtgg aaagtaacat caatgttgtc taatttaata attggttctt
     7261 tactcatatg attctattaa agcctccact aattcaatat gggtgtaata atcagcaata
     7321 ctaacatttt catcaacgcc atggtctcga ctattggcat gtcctatgcc aatagctgca
     7381 attggcgctc ttagagcttg atgaactaaa tacattggtc ctgtacctgg tgaggtaggt
     7441 aacagagata tcccttctgg ataaaattgc tcagctaaat caacaacctt taggatagat
     7501 ggcgctgaca tatcactacg ataagacatt tcaccaaggg tataggttag ctcaatgtct
     7561 ttaaagccat tctctttcaa atgattttgt attgaatcta gtacaccttt cggggttaac
     7621 cctggtacta acctaacctc tgctttacat ttagcataag caggtaaaat tgttttgacg
     7681 ccttcacctt gataacctga ggttatacct tcaatggcta tagaaggttc aaaataaagc
     7741 tttctcaaaa aagttttatg actatctgct aaactgggca aaactaattg gtaagcacct
     7801 tctagcgcct ttgcagatct ataagaatat ttttcaacta attctaactc tctcttagta
     7861 gggggaataa ctttatcata aataccttct accaatatat gacctttgtt atcccttaac
     7921 gtattgaggg cttgaattaa ataccatgtc gaggaatcaa caaccccacc aaaggatgaa
     7981 tgaatatcaa catccgctga acttactgat aaatcaaaag tgacaatgcc tttatttcca
     8041 ccagcaattt ccaactgtcc tttaggattt ttagggccat cttcccacac gatcaaatca
     8101 gccccttgta gctgctcttg atacttttct aaataataat caaggcctac tgaagctgat
     8161 tcctctgctc cttccacaat aaaagtgata tctagaggaa gttccccttt gtgtcttgat
     8221 aaatattttt taactgctga taaacgtgcg gtaatgtgac ctttatcatc atctacacca
     8281 cgtccataca tcttaccata tcgaagtgaa agtgtaaaag ggtcctcagt ccactgttct
     8341 acctcatctg ctggtacagt atcataatga ttgtaaaaaa taatacgctt agcatctact
     8401 tttgatgatt tgaaatttgc cacgataaag ggagcgctat agctgtcatc tataatgact
     8461 tctgcgccag cttttctgaa aacttctcca agatagcttg ataagtcatt taaaccaact
     8521 ttctgagcaa aaattgactt aattgcaatt aaatctctta aaatattgat atcatcctga
     8581 acaatagtat cagatagaaa cttttgaatt tgattttctt cgtttgttaa agtcatcttt
     8641 catccacctt ccttcaaaaa caatccaaga gcctggcaca ttgtattcgt cccaggccct
     8701 ttatggctat atggctgata agttgtatcc tattaggatt ccctgttaaa cttatacagt
     8761 caattcacgt taccattgtg gaatatcagc tgaagtatct ttgataactt ttttcacttc
     8821 atctgtgtga taagcatcca agatagcttg gatagcttta gcgttctttt gctttttcca
     8881 atttttacgt cccgcaatga tattaatcca ttgttttgaa tttttatctg atttctcaac
     8941 aaagatagca tctgaaggtt ttaaattagc ttgctcaatg tatgtattat taataatagc
     9001 tgcatctaca tctttgagtg cacgtggtgt ttgactcgca tctaactcct gaatattaat
     9061 atccttttta ttagatgtga tattagcaac tgttgcaacc ttcttaccag aaacattcaa
     9121 tttgattaaa cctgctgact gaaggacata caatgcacgg ctaccatttg ttgcatcatt
     9181 tggaattgca atagtggctc cttttttcaa ttttttaaga gattttacct tctcagaata
     9241 gatacgaatt ggagctaagt aagtcttttc aagtggaatt aagtttttct tattttcctt
     9301 attccagttt tctaagaaat tgtaatgttg aaaggcatta atatccacat ccttattggc
     9361 tgtcgcttga tttggttgtg tataatctgt aaattctgta aatttgattt tagctttatc
     9421 acctactagc ttttcaattt tatcccaacg tgctttttca gtgtcagaaa aggtcataac
     9481 accaacttta acaactttac ttgaagttga gcttgctgaa acttcttgat tactaaatgt
     9541 gactcctaaa agtgttgtta aggctaatcc taagacaata tgttttaact tcataataaa
     9601 ttccct
//
LOCUS       DATROD010000071         9411 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.071, whole genome shotgun sequence.
ACCESSION   DATROD010000071 DATROD010000000
VERSION     DATROD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9411
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.071"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(46..1365)
                     /locus_tag="VBC74_001465"
     CDS             complement(46..1365)
                     /locus_tag="VBC74_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEO6942405.1"
                     /translation="MKKYDVIVLGFGKAGKTLAAKLATQGKSVAMVEEDDKMYGGTCI
                     NIGCIPTKTLLVSASKNHDFQEAMTTRNEVTSRLRAKNFAMLDNKDTVDVYNAKARFI
                     SNKVVELTGGADKQELTADVIIINTGAKSVQLPIPGLADSQHVYDSTAIQELAHLPKR
                     LGIIGGGNIGLEFATLYSELGSKVTVIDSQSRIFAREEEELSEMAQDYLEEMGISFKL
                     SADIKSVQNEDENVVISFEDEKLSFDAVLYATGRKPNTEGLALENTDIKLTERGAIAV
                     DEYCQTSVENIFAVGDVNGGPQFTYISLDDSRIVLNYLNGDKDYSLKNRGAVPTSTFT
                     NPPLATVGLDEKTAKEKGYQVKSNSLLVSAMPRAHVNNDLRGIFKVVVDTETNLILGA
                     RLFGAESHELINIITMAMDNKIPYTYFQKQIFTHPTMVENFNDLFNF"
     gene            complement(1519..2016)
                     /locus_tag="VBC74_001466"
     CDS             complement(1519..2016)
                     /locus_tag="VBC74_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEO6942406.1"
                     /translation="MTTYFDNFLKTNQAYADLHGTAHLPIKPKTKVAIVTCMDSRLHV
                     AQALGLALGDAHILRNAGGRVTADVLRSLVISQQQLGTREIVVLHHTDCGAQTFTNEA
                     FAAQLQRDLGVDMHGHDFLPFNDIEESVREDVAKLHASPLIPNDVVISGAIYDVDTGR
                     MVEVK"
     gene            complement(2152..3516)
                     /gene="radA"
                     /locus_tag="VBC74_001467"
     CDS             complement(2152..3516)
                     /gene="radA"
                     /locus_tag="VBC74_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEO6942407.1"
                     /translation="MAKKKSVFTCQECGYQSPKYLGRCPNCSAWSSFVEEVEVQEVKN
                     ARVSLNGEKSRPTKLKDVSSINYSRTKTDMDEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYVSGEESAEQIKLRSERLGDIDNEFYLYAETNMQSIRSEIE
                     KIKPDFLIIDSIQTIMSPEVSSVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPTVFGNAKRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQESFIGE
                     IGLTGEIRRVTRIEQRINEASKLGFTKIYAPKNSLAGIEIPKGIDVIGVTTVSQVLKA
                     VFST"
     gene            complement(3678..4124)
                     /locus_tag="VBC74_001468"
     CDS             complement(3678..4124)
                     /locus_tag="VBC74_001468"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEO6942408.1"
                     /translation="MSKVRGFELVSQFSNKELLPKRETAHAAGYDLKVAKKTVIEPGE
                     ITLVPTGIKAHMQPGEVLYLYDRSSNPRKKGIVLINSVGVIDGDYYNNQVNEGHIFAQ
                     MQNITDQAVILEEGERIVQAVFAPFLLADDDQATGMRTGGFGSTGK"
     gene            complement(4187..4272)
                     /locus_tag="VBC74_001469"
     tRNA            complement(4187..4272)
                     /locus_tag="VBC74_001469"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4236..4238),aa:Leu,seq:aag)
     gene            complement(4319..5245)
                     /locus_tag="VBC74_001470"
     CDS             complement(4319..5245)
                     /locus_tag="VBC74_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEO6942409.1"
                     /translation="MKKIRLSKFIKMIVVILFLISVAASFYFFHVAQVRDDKSFISNG
                     QRKPGNSLYAYDKSFDKLLKQKIEMTNQNIKQVAWYVPAAKKTHKTVVVVHGFANSKE
                     NMKAYGWLFHKLGYNVLMPDNIAHGESHGQLIGYGWNDRENIIKWTEMIVDKNPSSQI
                     TLFGVSMGGATVMMASGEKLPSQVVNIIEDCGYSSVWDELKFQAKEMYGLPAFPLLYE
                     VSTISKIRAGFSYGQASSVEQLKKNNLPALFIHGDKDNFVPTSMVYDNYKATAGKKEL
                     YIVKGAKHAKSFETEPEKYEKRISSFLKKYEK"
     gene            complement(5354..6958)
                     /locus_tag="VBC74_001471"
     CDS             complement(5354..6958)
                     /locus_tag="VBC74_001471"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEO6942410.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLKKE
                     RRGEYLGATVQVIPHVTDALKEKIKRAATTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSDNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEQPAG
                     QSIKNKLAQFCDVAPEAVIESLDVDHIYQIPLNMQAQNMDQIVCDHLKLETPAADMTE
                     WSAMVDKVMNLEKKVKIALVGKYVELPDAYLSVVEALKHSGYVNDVAIDLKWVNAAEV
                     TEDNIKELVGDADGIIVPGGFGQRGSEGKIEAIRYARENDVPMLGVCLGMQLTCVEFA
                     RNVLNLHGANSAELDPKTPFPIIDIMRDQIDIEDMGGTLRLGLYPCKLKAGSRAAAAY
                     NNQEVVQRRHRHRYEFNTKFREQFEAAGFVFSGVSPDNRLMEVVELPEKKFFVAAQYH
                     PELQSRPNHAEELYTAFVTAAVENMK"
     gene            complement(7231..7815)
                     /locus_tag="VBC74_001472"
     CDS             complement(7231..7815)
                     /locus_tag="VBC74_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEO6942411.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILEQRGRDNEMYFSDLVNDIQT
                     YLGKSDSAIRESLPFFYSDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     APKRKKKRVNAFMDGDEDAIDYNDDDPEDEDFTEETPSLEYDEENPDDEKSEVESYDS
                     EINEIIPDEDLDEDVEINEEEEEEEEEEEEEEEV"
     gene            complement(8004..9287)
                     /locus_tag="VBC74_001473"
     CDS             complement(8004..9287)
                     /locus_tag="VBC74_001473"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEO6942412.1"
                     /translation="MSTSFENKATNRGIITFTISQDEIKPALDQAFNKVKKDLNVPGF
                     RKGHMPRTVFNQKFGEEALYENALNLVLPKAYEAAVAELGLDVVAQPKIDVVSMEKGQ
                     DWKLTAEVVTKPEVKLGDYKDLSVEVDASKEVSDEEVDAKVERERNNLAELTVKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEEQLVGSKAGQTVDVNVT
                     FPEDYQAEDLAGKDAKFVTTIHEVKTKEVPALDDELAKDIDDEVETLDELKAKYRKEL
                     ESAKEIAFDDAVEGVAIELAVANAEIVELPEEMVHDEVHRAMNEFMGNMQRQGISPEM
                     YFQLTGTTEEDLHKQYQADADKRVKTNLVIEAIAAAEGFEATDEEIEKEITDLASEYN
                     MEADQVRGLLSADMLKHDIAMKKAVDVITSSATVK"
BASE COUNT         2878 a         1993 c         1439 g         3101 t
ORIGIN      
        1 gataaaaaaa gtaaagcaaa attgccttac tttttaaaaa tattcttaaa agttaaacaa
       61 atcattaaaa ttctctacca ttgttgggtg agtgaaaatt tgtttttgga aataagtata
      121 tgggatttta ttatccattg ccattgtgat gatattaatt aattcatggg attctgcacc
      181 aaataagcga gctcccaaaa taagatttgt ctcagtatct accacaactt taaaaatacc
      241 tctaaggtca ttattaacat gtgcacgtgg catggcagaa acaagaaggc tattagattt
      301 aacttgatat cctttttcct tagcggtctt ttcatctaga cctaccgttg ctaaaggagg
      361 atttgtaaag gttgaagtcg gtactgctcc tcgatttttt agagaataat ccttatcacc
      421 attcaaataa tttaagacaa tacgtgagtc atctaaagaa atatatgtga attgagggcc
      481 gccattaaca tcaccaactg caaaaatatt ctcaacagaa gtttggcaat attcatcaac
      541 agcaatagct ccacgttctg tcaatttaat atctgtattt tcaagtgcta aaccttctgt
      601 atttggctta cgtcctgttg catataatac tgcatcaaag cttaattttt catcctcaaa
      661 gctaataaca acgttttcat cttcattttg cacagatttg atatcggctg ataatttaaa
      721 agatatcccc atctcttcta aataatcttg agccatctct gataattcct cttcctcacg
      781 cgcaaagata cgagattgac tatcaataac tgtcactttg ctccctaatt cggaatataa
      841 agtcgcaaat tcaagcccaa tattaccgcc tccaatgata cctaaccgtt taggaagatg
      901 agccaattct tggattgccg ttgaatcata aacatgttga gaatcagcta atcctggaat
      961 tggtaattgt actgacttag caccagtatt gataataata acatcagctg taagttcttg
     1021 tttgtcagct cctcctgtta attcgaccac tttatttgat ataaagcgag ctttagcatt
     1081 ataaacatcc acagtgtctt tattatccaa catagcaaaa tttttagcac gtaaacgact
     1141 tgtaacttca tttctagtag tcatagcttc ttgaaaatcg tgatttttgc tagctgatac
     1201 taaaagcgtt tttgtcggaa tacatccaat gttgatacat gttcccccat acattttatc
     1261 gtcttcttcg accattgcaa cgcttttacc ttgtgttgct aattttgctg ctaatgtttt
     1321 acctgcttta ccaaaaccaa gaacaatgac atcatatttt ttcattttaa atttcctccg
     1381 cttttataat atagtttcag tatagcaaaa gatgtttcct ctgtctgaat tccagcataa
     1441 ggcatgataa gcctaactgt tacctttaaa tctagttttc taaaataaaa aaacccatcg
     1501 ctaaggacag gattttttct atttaacttc caccatacga ccagtatcta catcataaat
     1561 agcaccagaa ataacaacat catttgggat taatggagaa gcatgtaatt ttgccacatc
     1621 ttcacgaacg ctttcttcaa tatcattaaa aggaagaaaa tcatgtccat gcatatcaac
     1681 acccaaatct ctctgtaatt gagcagcaaa agcttcgtta gtaaacgtct gtgctccaca
     1741 atctgtatgg tgtagaacaa cgatttctcg tgttcctaat tgttgttgcg aaataactaa
     1801 agaacgtaga acatcggctg taactcgtcc accagcatta cgtaaaatat gagcatctcc
     1861 taaagctagc cctagagctt gagcaacgtg taaacgagaa tccatacagg ttacaatagc
     1921 aacctttgtc tttggcttga taggtagatg agctgttcca tgtaagtcag cataggcttg
     1981 attggttttt aaaaagttat caaaataagt ggtcatagag atctcctttg ttagtgtaac
     2041 atcattcgat atttactaaa taccaaccct tttatattat cattttgccc attatttttc
     2101 acgaaatgtc tctcgccacc aaaaaagatt cggctgaacc gaatctaatc attatgttga
     2161 aaacactgct tttaagacct gactaacagt tgtcacacca ataacatcaa tgcctttagg
     2221 tatttcaatc ccagctagag aattctttgg tgcgtaaatt ttagtaaagc ccaatttact
     2281 agcctcatta atacgttgct caatacgtgt cacgcgacga atctcaccag tgagtccaat
     2341 ttctccaata aatgattctt gtggatttgt gggtttttct ttgtaactgg aagcaatagc
     2401 tacagcaaca gctaaatcaa ttgctggttc atccaattta acaccacccg ctgattttaa
     2461 ataggcatct tgattttgaa gcaataaacc acaacgtttt tctagaactg ccataataag
     2521 actgacacga ttaaagtcaa gacctgtggt tgtgcgctta gcatttccaa aaacagttgg
     2581 agtcactaaa gcttgaacct ctgctaaaat aggccttgtt ccttccatag taacaacaat
     2641 agcagatcct gttgcaccgt ctaaacgttc ttcaagaaaa acttgacttg gattcaaaac
     2701 ttcaactaga ccacccgact gcatttcaaa gatacctatt tcattggtcg aaccaaaacg
     2761 gtttttgact gcccgtaaaa tacggaacgt gtgatgacgc tccccttcaa aataaagaac
     2821 cgtatccacc atatgttcta gcattcgagg gcctgctaat gttccctcct tagtcacatg
     2881 acctacaata aaagtagcaa tattattagt tttagctaac tgcatcaact cagcggtcac
     2941 ttcgcgaact tggctaacag agccttggac acttgatact tcgggactca taatagtttg
     3001 tatagaatca ataataagaa aatctggttt aatcttctca atctccgacc gaatactttg
     3061 catattagtc tcggcataaa ggtaaaattc attatcgata tcgcctagac gctcacttcg
     3121 taacttaatc tgttctgcag attcctcacc tgagacatat aatactgtac ctttattagc
     3181 tagctgagtt gatacttgta gtaataaagt agatttacct atcccagggt ctcctccaat
     3241 cagaaccaaa cttcctggta caacaccgcc tcctagaaca cggttaaatt catccatatc
     3301 agtcttagtt ctagaatagt taattgagga aacatctttt aacttcgtcg gtcgggactt
     3361 ttcaccgttc aaactaactc gtgcattttt aacttcttga acttcaacct cttctacaaa
     3421 tgaagaccag gctgagcaat ttggacaacg tcccaaatac ttaggtgatt ggtaaccaca
     3481 ctcttgacag gtgaagactg attttttctt agccactcag gcctccttta taacttctaa
     3541 attaatatga tagcttggta ccaaataact agtacactca ttatatcaca ccaagttaga
     3601 gaaaatataa gtttactctc atcaaaaaag gctaagaagt aagtctagcc ttttctaaca
     3661 ttattcgtaa aataatttta ttttccagta ctaccaaaac cacctgtacg cattcctgta
     3721 gcctgatcgt catctgccaa taagaatggg gcaaaaacag cctgaacaat ccgttcccct
     3781 tcttctaaaa taacagcctg atcagtaata ttttgcatct gtgcaaaaat atgtccttca
     3841 ttgacttggt tattatagta gtcaccatca ataacaccaa ctgaatttat taggacaatc
     3901 ccttttttac gtggattcga tgaacgatca taaaggtaca ggacctcccc tggttgcatg
     3961 tgtgctttaa taccggtcgg gaccaatgta atttcaccag gttcaataac cgttttttta
     4021 gctactttta aatcatagcc agctgcgtga gctgtttctc gttttggtaa taactcttta
     4081 ttgctaaatt ggctaactaa ttcaaagcca cgtacttttg acatgtgata ctccatcttt
     4141 atcttaaata tgcaaaaagt cgagttccaa atgaactcga ctagtctgcg gagagaggga
     4201 cttgaaccct cacgaccaaa agcggtcaca agatccttag tcttgcgcgt ctgccaattc
     4261 cgccatcccc gcttaacaac ataataaatt atatcaactt atcatattgt tgtcaatatt
     4321 atttttcata ttttttcaaa aaactagaga tacgtttctc atatttttct ggctctgttt
     4381 caaaagattt cgcatgtttt gcccctttta caatataaag ctctttctta cctgctgtag
     4441 ctttatagtt gtcataaacc atacttgttg gaacaaaatt atccttatca ccatgaataa
     4501 agagggctgg taaattattc tttttcaatt gttcgacact acttgcttgt ccatacgaaa
     4561 aacctgctct gattttagaa attgttgaaa cttcatataa gagtgggaag gctggtaaac
     4621 catacatctc tttagcctga aattttaatt catcccaaac actagaataa ccacaatctt
     4681 caatgatatt aacaacctga ctaggtaatt tttcaccact agccatcatg actgttgctc
     4741 cacccattga aacaccaaat aaagtaattt ggcttgatgg attcttatcc actatcattt
     4801 ctgtccattt gataatgttc tcgcggtcgt tccagccata gcctatcaac tgcccatgac
     4861 tttcaccatg tgcaatgttg tcaggcataa gaacattgta tcctaactta tgaaacagcc
     4921 aaccatatgc cttcatattc tctttgctat tcgcaaaacc atgaacgaca acaactgtct
     4981 tatgagtttt cttagcagca ggaacatacc aagcaacttg ctttatattt tggtttgtca
     5041 tttctatttt ttgctttaat agcttatcaa aggatttatc ataagcatat aaagagtttc
     5101 caggcttacg ttgaccattt gaaataaagg atttatcatc tcgaacttgg gcaacgtgga
     5161 aaaaataaaa actagctgct acactaatta aaaacaaaat aacaacaatc attttaataa
     5221 actttgataa tcgaatcttt ttcatgcaac ctattatacc ttatttttag aaaaagaaaa
     5281 gagctaataa tactttgata gtgaaactat ctaaaaaaat aaaatcgcct cctaatactg
     5341 ggaggcgata aagttatttc atattttcaa ctgctgctgt tacaaaagct gtgtaaagct
     5401 cttcagcatg gttaggacgt gattgtagtt ctggatggta ttgtgctgca acaaagaatt
     5461 ttttctctgg taactcaaca acttccatca aacgattatc cggtgatacc cctgagaaga
     5521 caaatccagc agcctcaaat tgctcacgga attttgtatt aaattcataa cggtgacggt
     5581 gacgacgttg gacaacttct tgattattat aagctgccgc agctcttgaa cctgctttca
     5641 atttacatgg atatagacca aggcgaagtg taccacccat atcttcaata tcgatttgat
     5701 cacgcatgat atcaatgatt gggaacggtg ttttagggtc aagctctgct gaattagctc
     5761 catgaaggtt taaaacattg cgcgcaaatt cgacacaagt tagctgcatt ccgagacaaa
     5821 caccaagcat tggaacatca ttttcacgtg catagcgaat tgcttcaatt ttaccctcac
     5881 tacctctttg gccaaatcca ccaggaacga tgataccatc tgcatctccg acaagttctt
     5941 taatattatc ttctgtcact tcagcagcat ttacccattt taggtcaata gcaacatcgt
     6001 tgacgtaccc tgagtgctta agggcctcca caactgaaag gtaagcatca ggtaattcaa
     6061 catatttacc aaccaatgca attttaactt ttttctcaag gttcattact ttatcaacca
     6121 ttgctgacca ttctgtcata tcagcagcag gtgtctcaag cttcaagtga tcacaaacaa
     6181 tttgatccat attttgagct tgcatgttaa gtggaatttg ataaatatgg tcaacatcaa
     6241 gggactctat aacagcctct ggtgcaacat cacaaaattg tgccaattta ttcttaatac
     6301 tttgacctgc tggttgctct gtacggataa caagcatatt aggttggata cctaggccac
     6361 gtaactcttt aacagaatgt tgagtcggtt ttgttttcat ttcgccggca gcctttaggt
     6421 atggtaataa cgttgtatga atgtacataa cattatcaga accaacatct gccttcattt
     6481 gacgaagtgc ttctaaaaac ggaaggctct caatatctcc aacagtaccg ccaacttcag
     6541 taataataac atctgaatct gtagttgtcg cagcacgttt gattttttct ttcaaagcat
     6601 ccgtaacgtg tgggataact tgaacagttg caccaaggta ttctccacgg cgttcttttt
     6661 taagaacttc actataaatc ttacccgttg taacatttga atacttgttt aagttaatat
     6721 caataaaacg ctcgtagtgt ccaaggtcaa ggtcagtttc tgcaccatca tctgtaacat
     6781 aaacttcccc atgttgataa gggctcattg tccctgggtc aatattaata tatggatcaa
     6841 atttttgaat tgtcaccttt agcccacggt tttttagtaa gcgcccaaga cttgctgcaa
     6901 caatcccctt accaatagaa gagaccacac cacctgtaac aaaaatatac tttgtcatta
     6961 aaaactcctt aaattaaatg ttaatctaac agttagttta cgggtaaaac ccaaaacaca
     7021 tgattgataa ataataccag cttctctagt caaaactagt agaaactatc tgatcaaggg
     7081 cctactcaga aaactatctg gaaccattct gtatctttcc caaaaacaaa atagccccct
     7141 atcactaggg agctttatct gacctcataa gaggtgcccg aataatagtt taaactatat
     7201 ctgactattt gtcaaataca aaatccccat ctaaacctct tcttcctctt cttcctcttc
     7261 ttcctcttct tcctcttctt cgttgatttc tacatcctca tctaaatctt catcaggaat
     7321 gatttcatta atctcagaat catacgattc tacctctgat ttttcatcgt cagggttttc
     7381 ttcatcatat tctaaacttg gtgtttcttc tgtgaaatct tcatcttctg gatcatcatc
     7441 attgtaatca atagcatctt catcaccatc catgaaagca ttgacacgtt tcttcttacg
     7501 ttttggagcg ccatcttcat cttcttcaag agtgatgatt tcctcgtcaa tttcatcgat
     7561 agcataccat gaacgaagtc cccatttgtt ttcgccaagt ggaataaaac ttccgtctgt
     7621 attcaaatct gaatagaaaa atggcaatga ctcacggatt gctgagtcag attttccaag
     7681 gtaggtttga atatcattta ccaaatcgct gaagtacatt tcgttatcac gaccacgttg
     7741 ctctaaaata gcgcgagcaa cttcaatcat cgaaagttca cttttttctt gcccagcaaa
     7801 tacttctaat tccaaagtta ttctccttaa ttgtgtaaga ctatcatttt gtttagcaat
     7861 attctttata gatagaaaat agataagtca tattttcaaa ttttcgctat ttactatttt
     7921 acgctatttt tagctattcg tcaaaagaat aatcacaaat aaaaagactg ggaagcccaa
     7981 tcttttcaaa atttaataag caattatttt acagtagctg agctagtgat aacgtcaacc
     8041 gcttttttca ttgcaatatc atgtttaagc atatctgctg aaagtagacc gcgtacttgg
     8101 tcagcttcca tattatattc tgaagcaaga tctgtgattt ctttttcgat ttcttcatct
     8161 gttgcttcaa agccttctgc agctgcaatt gcttcaataa caaggttagt tttaacgcgt
     8221 ttgtcagcat ctgcttggta ttgcttatga aggtcttctt cagttgtacc tgttaattgg
     8281 aagtacattt ctggtgaaat tccttgacgt tgcatattac ccatgaactc attcatagca
     8341 cgatgtactt catcatggac catttcttct ggtaattcaa caatttctgc attggcaaca
     8401 gctaattcga ttgctacacc ttcaactgca tcatcaaatg caatttcttt tgctgattct
     8461 agctctttgc gatatttcgc tttcaactca tcaagtgttt caacttcatc atcaatatct
     8521 tttgcaagtt catcatcaag tgctggaact tctttagttt ttacttcatg aattgttgta
     8581 acaaatttag catctttacc agcaagatct tctgcttgat aatcttctgg gaatgttaca
     8641 ttaacgtcta cagtttgacc agctttagaa cctactaatt gttcttcaaa tcctgggata
     8701 aattgacctg aaccaagttc aagtgagaag ttatctcctt taccaccatc aaattcaaca
     8761 ccatcaactg aacctacaaa gtcaataaca actgtatcac cttgtgctgc ttcaccatct
     8821 ttaacagtca attctgcaag gttgttacgc tcacgttcaa ctttagcatc aacttcttca
     8881 tctgaaactt cttttgaagc atcaacttct actgaaaggt ctttgtaatc cccaagttta
     8941 acttctggtt ttgtaacaac ttcagctgtc aacttccaat cttgaccttt ttccattgat
     9001 acaacatcaa tttttggttg agcaacaacg tctaaaccaa gttctgcaac agctgcttcg
     9061 taagcctttg gcaacactaa attcaatgca ttttcgtaga gtgcttcttc accaaatttt
     9121 tggttgaaaa ctgtacgagg catatgtcct ttacggaatc ctggaacgtt caaatctttt
     9181 ttaactttgt tgaatgcttg atcaagagct ggcttgatct catcttgact aattgtaaat
     9241 gtgataatac cacggtttgt agctttattt tcaaatgatg tagacattaa gtcatttctc
     9301 cttaaaatta tttagataca gtttagttta ccataattta aaacgtttta caaagttttt
     9361 atgcaaaaaa gaagctttaa actgcttaac gataagcttt taaaatactt g
//
LOCUS       DATROD010000072         9331 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.072, whole genome shotgun sequence.
ACCESSION   DATROD010000072 DATROD010000000
VERSION     DATROD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9331
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.072"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            197..388
                     /locus_tag="VBC74_001474"
     CDS             197..388
                     /locus_tag="VBC74_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO6942413.1"
                     /translation="MPDIANGRKKICDFLKENNIKKAALAAAYGMSRQEVTNILSGST
                     RGLKANQFILRVIEDYKID"
     gene            422..1192
                     /locus_tag="VBC74_001475"
     CDS             422..1192
                     /locus_tag="VBC74_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEO6942414.1"
                     /translation="MNEIFNFHGQEVRTVTINNEAYFVGKDVADILGYSNGSRDVNRH
                     VEEEDRLKYRFGTSGQDREMTIINESGLYSLILSSKLPQAKDFKRWVTSEVLPTIRKH
                     GLYAIDDLLENPDMAIAALQKLKEERQLRLQAQEEVAQKNQIIQELQPKATYYDLILQ
                     SESLVAISVIAKDYGMSAKKLNSLLHELKVQYKQGNTWLLYQKYASKGYTQSKTHPID
                     AERSKMHTYWTQKGRLFIYDLLKNKKGILPLIEQEEVA"
     gene            1270..1581
                     /locus_tag="VBC74_001476"
     CDS             1270..1581
                     /locus_tag="VBC74_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEO6942415.1"
                     /translation="MPKAEIIYRPANQSEKATHGDYAHLCQVWEGLTIGTAKVWAAEM
                     REHPDFRQFIDNPTHRIVFIDYEGFRLFVKWKSRNRYRPKKETLSEMIDNIKREKQLG
                     V"
     gene            1586..1732
                     /locus_tag="VBC74_001477"
     CDS             1586..1732
                     /locus_tag="VBC74_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942416.1"
                     /translation="MKKLLKKLFVKKNRKIDVRPAWKIETHGWEANARRYDNMIEHNR
                     GRTC"
     gene            1861..2272
                     /locus_tag="VBC74_001478"
                     /pseudo
     CDS             1861..2272
                     /locus_tag="VBC74_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2419..2622
                     /locus_tag="VBC74_001479"
     CDS             2419..2622
                     /locus_tag="VBC74_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942417.1"
                     /translation="MNYLEYALAYLERELEIIDNEAIEVELPDGDWEFVLNPYYEEGL
                     HDNPYYRSQVAKDILDIKGLLGR"
     gene            2712..3011
                     /locus_tag="VBC74_001480"
     CDS             2712..3011
                     /locus_tag="VBC74_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942418.1"
                     /translation="MIEVTFKAESMAGVFDAMREALDMPKTVTKDVVEEKPAPKKKEE
                     SVTLTLADIKKMAKAKVEEGKSKDIKEVLREMNVAKVGELEEYQFAEFVEKLEAL"
     gene            3011..4168
                     /locus_tag="VBC74_001481"
     CDS             3011..4168
                     /locus_tag="VBC74_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015986706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HEO6942419.1"
                     /translation="MPVENHALLSASSAHRWLYCPMLPRLEADYPSRDTVYTQEGTSA
                     HELSEIKLMYKSGKITKRKFNTLTKAFKENSDFYNEEMEEMTELYTDIVMEHLNAYEN
                     AEIELEKRVDFSDWVPGGFGTSDVVILADGVIEIIDLKYGKGMPVSANQNPQMGLYAL
                     GAYASYDMVYDFDRIKMTIIQPRLDSVSSVDIYVEELLYWADNVVLPMAAQADAGIGD
                     WNLSEKVLQWSPVAAKLVPRAQENWELIDKYDYQEPVYLSDEAVAEILDKASAIKKWV
                     ESVEAYALKEALSGKEVPGYKIVEGRSNRVITDKDKAIDILQDNGFDDEIFKPKELLS
                     MGALEKLIGKTTFADLLAEVIDKPQGKPVLVPEKDKRPAINSLEQAIKDFE"
     gene            4182..4745
                     /locus_tag="VBC74_001482"
     CDS             4182..4745
                     /locus_tag="VBC74_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HEO6942420.1"
                     /translation="MTTTPNTTKVVTGKVRLSYVALLEPKAFEGQEAKYSTVILIPKT
                     DKATIKKIKDAQKAAYEAAKDNKLKGVKWERVKTTLRDGDEEMDTEEHPEYAGHMFMS
                     ISSKTKPQIIDKYKNSVDSAEEVYSGVYARVSLNAYAYNTAGNKGISCGLNNVQIVAK
                     GDYLGGRSSADADFDEWNEEEDEDDIL"
     gene            4788..6710
                     /locus_tag="VBC74_001483"
     CDS             4788..6710
                     /locus_tag="VBC74_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEO6942421.1"
                     /translation="MRHLNIDIETYSSNDIKNGVYKYADAEDFEILLFAYSIDGGEVE
                     CLDLTRQSLPEDIKDMLFDDKVRKHAFNAQFERVCLSRYLGLPYYLDPCQWQCTMVLA
                     QELGLPSSLEKCALYLKLAQEKDTSGKNLIRYFSLPCKPSKTNGGRTRNLPEHAPEKW
                     QMFIDYCIQDVVVEMAIAEKLESVPVHDREWDYYVCDQRINDRGVALDKELVASALYC
                     KDVKMESLSGELKALTGLDNPNSRAQLLPWLKEHGYSANGLTKADVERELKTAEGELK
                     RVLELKLQTAMSSLKKYEAMERAMCSDGRVHGLLQFYGASRTGRWAGRVVQVQNLARN
                     YIKDLDDAREYVKKRDIDAVEILYDSLNDTLKQLVRTALVAKDCCTFYVSDFSAIEAR
                     VIAWFAGEQWRLDVFSTHGKIYEASASQMFGIPIEEIDKELRQKGKISELALGYQGGP
                     GALKQMGALNMGVKEEELQGLVDDWRRTNKKIVQFWKDVQRAAIKAIKSRAPIKLGKL
                     RFRYRKGFLFITLPSGRNLAYARAKVEPGDFGDKIIYEGQGDKAYFTAQETYGGKLVE
                     NIVQATARDILAEALLRIEAAGHGVVFHVHDEAIIEGSGLTIEEVNELMAQAPDWAEG
                     LPLNSEGYITKYYMKD"
     gene            6715..9099
                     /locus_tag="VBC74_001484"
     CDS             6715..9099
                     /locus_tag="VBC74_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEO6942422.1"
                     /translation="MKQEKLIVKSSPLQELHIATGNSRTAKTWKNITLTWQELVERLE
                     KPTVTQETFAEYQKMSRAEKGQAKDVGGFVGGWLKQGKRKNENVQSRSLVALDADSPS
                     KDFLDRLDLLADYAYVLYSTHSHSKKAAKYRLIIPTDRLMMPDEYEPVARYLANQLGM
                     SNFDDTTYQSVRLMFWPSHSRDADFTFKYNDEAFLSVDEVLDTYPDWHDSSFWPESPT
                     HAVKRQREAKKQGDPLSKKGLIGAFCRNYDIRQAIATFLPEVYEEGTTPDRYTYTEGS
                     TANGLVIYDDVFAYSHHGTDPVGDTLVNAYDLVRIHKFGEQDSGAKDNTPTNKLPSSK
                     AMNAFVCDLPEIKDYLMAEALGDFDEELPVEDDRSWLEIDERGEPEVNSYLLATQIIK
                     EVPIYWDGFEFLRYDAKKGIWLPNAEEYLKSYISTKKLGKITKIRHISETIVAIKAQA
                     FSSEVFTESDLNKIVLANGVYDLRDNSFKTKFDPELHARSSHPVVYDPEATCETFEGF
                     LRETVGAENIDFIFEWFGYNFYREYTIQKMLFIYGSGGTGKSTLINILREMIGADNYS
                     AVTLQYLMQERFAKIGLYRKTANFDTDAKPQYLADGATLKMLTGEDTIHADRKNKEPI
                     NFYNYAKLSFAMNELPPMRDFSGGLKRRMMILEMDKVLTQEVKAKYPLDKIMGEVSGI
                     FNRAMEGLRKALSKRDFSISASMRSSVEKWEKGNDVVAMFLEDECEPGEDFKAPVRDV
                     YPAYKFYCQDSGYKPLAKNAFNHRLRELNYENKNVKSGGKQAKNWVGFRLKSEF"
BASE COUNT         3126 a         1648 c         2161 g         2396 t
ORIGIN      
        1 ttttgtaaat aagatttaac tagaaaaaca cctaaacaaa taaatcaaaa aaaagtgttt
       61 tagcgaacgg tattcattga tttattagct tctttttcta tgctttcatt ttagaatatt
      121 ctctaaaata tgtcaataaa aaacgcaaaa aaatagttgt ttcttattta caattttaaa
      181 taaaggagga agaaagatgc ctgatattgc aaatggacgc aaaaaaattt gtgacttctt
      241 aaaagaaaac aatatcaaaa aagcagcttt agcagctgca tatggcatga gtcgacaaga
      301 agttactaat attttaagtg gctcaactcg tggcctgaaa gctaatcaat ttatcttacg
      361 agttatcgaa gattataaaa ttgattaaat cgcaggaaga gtttgaagaa agggataatg
      421 tatgaacgaa atttttaatt ttcacggaca agaagtccgc acagtaacaa tcaataatga
      481 agcatacttt gttggtaaag atgtggcaga cattcttggt tactcaaacg ggagccgaga
      541 tgtaaataga cacgttgaag aagaagatag gctaaagtac cgttttggta cctcaggtca
      601 agatagagaa atgactatca tcaatgaatc aggtctttac tcacttatct tatctagcaa
      661 gttaccgcaa gcaaaagatt ttaaacgttg ggttacgtca gaagtcttac cgacaatccg
      721 taaacatgga ttgtacgcta ttgatgatct gctggaaaat ccagacatgg caatcgcagc
      781 acttcagaag ctcaaggaag aacgtcagct acgtttacag gcacaagaag aagtagctca
      841 aaagaatcaa attattcagg aattacaacc aaaagcaact tattatgatt tgattttaca
      901 aagtgaatcc ttagtggcta tctctgtcat tgcaaaagat tacggcatga gtgctaagaa
      961 actaaatagt ctgttgcatg aattgaaagt tcaatataag caaggcaata cttggctact
     1021 atatcagaag tatgctagca agggttacac acaatccaag acgcatccga ttgatgcaga
     1081 acgcagtaag atgcacactt actggacaca aaaaggacgc ctgttcatct atgacttgct
     1141 aaaaaacaaa aaggggattt tgccattgat tgagcaggaa gaagtggcgt aataaaaaaa
     1201 gcacctaacg aagtcaggcg ctcaacaaaa ataactaact agattataac acagaaaaga
     1261 gagtttaaca tgccaaaagc agaaattatt tacagaccag ctaatcagtc tgaaaaggca
     1321 acgcatggag actatgccca tctttgccag gtttgggaag gtttgacaat cggaacggcc
     1381 aaggtttggg cggcagaaat gcgagaacat ccagacttta ggcagtttat tgacaatcca
     1441 acgcaccgta ttgtctttat tgattacgag ggtttccgtc tattcgtcaa atggaagagt
     1501 cgcaatcggt acagaccgaa aaaagaaaca ttgtctgaaa tgatagacaa cattaaacgc
     1561 gaaaaacaat taggagtata acatcatgaa gaaattatta aaaaaactat ttgtaaaaaa
     1621 aaatcgaaaa atagatgtgc gtccagcatg gaagattgaa acacatggct gggaagctaa
     1681 tgcacgacgt tacgacaata tgattgaaca taatagaggg cgtacatgtt aaaagaagta
     1741 ttactagaaa atgaactgtt gcgtgatgaa aatagacgac taactaatga aatggcagat
     1801 tattatttta cgaacgtagc aaaagcaaat ttattggata ttatcgtcaa tgaaggctac
     1861 atcttagata gcacactgga aaaatgtatt gctaagctag ataacgttga tagacaaata
     1921 ctagaaaaaa attggagtga tgataatgat tgaagttaaa agacgagata gcttacctat
     1981 cctatctgct cagttgagtg ttatcaagca agatttaaaa ttgatgcttt ggattgagct
     2041 accgacgtta aatgacgtag agaaatatga agctctttat caaggaaatg attttaaatt
     2101 cagtgaaact tttttggagc gtcaagcgat gagaaaagcc ttttggagac gattagctaa
     2161 ggaaactttt aaagaagacc aggataggag gaactttgtt aagttggctg agaaagaatt
     2221 taaaggtgca actttgtcat ggcaagaatt tttgtggggt aaaaagaaat gaatatttca
     2281 ataaaactca taggtgaaac cctggctggt ttaaatgata ttttacgccg ggggggggct
     2341 ctcttgctcc caaaaccaag ccttagcaga cgcagttttt atattaacaa ctttaaaaca
     2401 agtaattgag gagagaaaat gaactactta gaatacgcac tagcttatct tgaacgtgag
     2461 ttagaaatta ttgataacga agctatcgaa gttgagttgc cggacggaga ttgggagttt
     2521 gtgcttaatc cctattacga ggaaggttta catgataacc catactatcg tagtcaggtt
     2581 gctaaagaca ttcttgatat taaaggactt ttggggaggt gaggctattg attgcatcaa
     2641 aaaagacggc ttaggaaacc gtcaacaatt acacacacac ttaaattata acacagaaag
     2701 agagaaacac aatgattgaa gtaactttta aagcagagag catggcaggt gttttcgatg
     2761 ctatgcgtga agctctggat atgccgaaga cagtaaccaa ggatgttgtt gaagaaaaac
     2821 cagctcctaa aaagaaagaa gagtctgtaa ctttaacatt agctgatatt aagaaaatgg
     2881 ctaaagccaa ggttgaagaa gggaagtcaa aggacatcaa ggaagtcttg agagagatga
     2941 acgtcgcaaa agttggtgaa cttgaagaat accagtttgc ggagtttgtt gagaagttgg
     3001 aggctctcta atgccagtag aaaatcacgc actactgtct gcttctagtg ctcacagatg
     3061 gctttattgc cctatgctac cgagattaga agcagactat cctagccgcg acaccgtata
     3121 cactcaagag ggcacaagcg cccacgagct ttctgaaatc aaactgatgt acaagtctgg
     3181 taaaatcacc aagcgtaaat ttaacacgtt aacaaaggct tttaaggaaa actcagactt
     3241 ctacaacgaa gagatggaag agatgacgga gctttacaca gatattgtta tggagcacct
     3301 aaatgcttat gagaatgccg aaattgaact tgaaaaacgg gttgatttta gcgactgggt
     3361 gcctggcggt tttgggactt cggatgtcgt cattttggcg gatggggtca ttgaaattat
     3421 tgatcttaag tatggcaagg gtatgcctgt gtctgctaac caaaacccgc agatggggtt
     3481 gtacgctctg ggagcttatg cttcctacga tatggtttat gactttgacc gtatcaagat
     3541 gaccattatt cagccacgtt tagattcggt tagttctgtt gacatttacg tagaggagct
     3601 tctgtattgg gcggataatg ttgtcttgcc tatggcagct caagcagatg caggcattgg
     3661 tgactggaac ctaagtgaaa aagtattgca gtggtctcct gtcgcagcta aattggtgcc
     3721 aagagcgcaa gaaaactggg aattaattga taaatatgac tatcaggagc ctgtctattt
     3781 atctgatgaa gccgtcgcag agatccttga caaagcctca gctatcaaga agtgggttga
     3841 gtcagttgaa gcctatgccc tgaaagaagc actctcagga aaagaagttc caggctataa
     3901 gattgtcgaa ggtagaagta atcgagttat cactgataaa gacaaagcaa ttgatatttt
     3961 acaagataac ggctttgacg atgagatttt taaaccgaaa gagctgttat caatgggagc
     4021 tcttgaaaag ttaataggta aaaccacttt tgctgattta ttagcagaag taattgataa
     4081 accacagggc aaacctgtac ttgtccctga aaaggataag cgcccagcaa taaacagttt
     4141 agaacaagca attaaagatt ttgaatagga gaaaaataat tatgacaaca acaccaaaca
     4201 caactaaagt agtgaccgga aaagtacgcc taagctatgt agctttacta gagcctaaag
     4261 cctttgaagg ccaagaggct aagtattcaa cagttatctt aattccaaaa acagacaaag
     4321 ccacaatcaa gaaaattaaa gacgcgcaga aagctgctta tgaagctgcc aaggacaata
     4381 aactcaaagg agttaaatgg gagcgcgtta agacaacgct tcgtgatggt gacgaagaaa
     4441 tggataccga agagcaccca gagtacgcag gccatatgtt catgtcaatc tcaagcaaaa
     4501 ctaaaccgca gatcattgac aagtataaaa actctgttga ttccgcagaa gaagtctact
     4561 ctggcgtcta tgctcgtgta tcacttaatg cctatgctta caacacagcg ggaaataaag
     4621 gaatctcttg cggattaaac aacgtccaaa ttgttgctaa aggagactac cttggcggcc
     4681 gttcgtcagc tgatgcagat tttgacgagt ggaacgaaga agaggacgaa gacgacattt
     4741 tatagttgag ggcctcttta gaggctcctc atttttaaag gagaggtatg agacatttaa
     4801 atattgatat tgaaacctat agttcgaatg acatcaaaaa tggggtttac aagtatgctg
     4861 acgcagaaga ttttgagatt ttacttttcg cttactctat agatggcgga gaagtagagt
     4921 gccttgattt gacaaggcag tctctacctg aagacatcaa agatatgtta tttgatgata
     4981 aagtccgaaa gcacgccttt aatgcccaat tcgaaagagt ttgtctcagt cgttacctcg
     5041 gcctacctta ctatctagat ccttgccaat ggcaatgcac catggtgctg gcccaagagt
     5101 tgggtctgcc ttcaagcttg gaaaagtgtg cgctgtattt aaaattagca caggaaaaag
     5161 atacctcggg taaaaactta atcagatact tttccctgcc ttgcaaacca agtaaaacta
     5221 acggtgggag aactagaaat ttaccagaac acgcccccga aaagtggcaa atgtttattg
     5281 actactgcat ccaagacgtt gttgttgaga tggcaattgc cgaaaaacta gagtcagttc
     5341 ctgtgcacga ccgtgagtgg gattactacg tctgcgacca gagaatcaac gacagaggcg
     5401 tggcgcttga taaagagtta gttgcttcgg ctttgtattg taaagatgtt aagatggaaa
     5461 gtttgtctgg tgaactaaaa gctctaacag gacttgataa tcctaatagc agggcgcagc
     5521 tgctaccgtg gctaaaagaa cacggttatt cggctaatgg gctgactaaa gcagatgttg
     5581 aacgggaact taagacggcc gaaggagaac ttaagagagt cttagaactt aaactacaaa
     5641 ccgcgatgtc aagtctaaaa aaatatgaag ctatggaaag agctatgtgc tcagacggac
     5701 gagttcatgg actacttcag ttttacggag ctagcaggac aggaagatgg gcgggcagag
     5761 ttgtccaagt acagaactta gctaggaatt atataaagga tctagatgat gctagagagt
     5821 atgttaaaaa gcgtgatatt gatgctgtgg agattttata cgatagtctt aatgacactt
     5881 taaagcagct agtaagaacg gcactcgtgg ccaaagactg ctgtaccttc tacgtctctg
     5941 acttctcagc gattgaggct agggtgattg cgtggtttgc cggagagcag tggaggcttg
     6001 acgtgttttc gacgcacgga aaaatctatg aggcatccgc tagccagatg ttcggaattc
     6061 caattgagga gattgataag gaactacgcc aaaaaggcaa aatctcagag ttggcacttg
     6121 gctatcaagg aggtcctgga gcgctcaagc agatgggggc tctaaatatg ggggtcaagg
     6181 aagaggagct tcaagggtta gttgatgact ggcgcaggac caataagaaa atcgtccaat
     6241 tttggaaaga cgtacaaaga gccgccatca aagccatcaa atcgagagca ccgataaaac
     6301 ttggaaaact acgatttaga taccgtaaag gtttcctctt tataacattg cctagcggta
     6361 ggaacttagc ttatgcaaga gccaaggttg agcctggtga ctttggagac aaaatcatct
     6421 atgagggcca aggagataag gcatacttca cagcgcaaga gacttatggc ggtaagcttg
     6481 tcgaaaatat cgttcaggcg acggctaggg atattctagc tgaagcgctt ctgaggattg
     6541 aagctgcagg ccatggtgtt gttttccacg ttcatgatga ggctattatc gaaggctcag
     6601 gcctgacaat cgaagaggtt aatgagttga tggctcaagc gcctgactgg gcggagggtc
     6661 ttcctttaaa tagcgaaggc tacataacaa agtattatat gaaggattag atagatgaag
     6721 caagaaaaac taatagtaaa gtcttctcct ctgcaagagc tccatatcgc aacgggtaat
     6781 tcacgaacag ctaagacgtg gaaaaatatt acgctaactt ggcaggagct ggttgagagg
     6841 ttagagaaac ctacagtcac ccaagagacg tttgcggagt accagaagat gtctcgagca
     6901 gaaaaagggc aagcaaaaga cgtagggggc tttgtcggtg gatggctaaa gcaaggtaaa
     6961 cggaaaaatg aaaacgttca aagtaggtcc ttggttgcgc ttgacgcaga tagcccaagt
     7021 aaagatttct tagataggct agacctgctt gcggattatg cctacgtact ctacagtact
     7081 cacagccact caaaaaaagc tgctaagtac cgtcttatta tccctactga ccgtttaatg
     7141 atgcctgatg aatatgagcc agtcgctaga tacttggcta accaactagg catgtcaaac
     7201 tttgatgaca cgacttatca aagtgtacgc ttgatgttct ggccgagtca ctcaagagat
     7261 gcagacttta cgtttaaata taacgacgag gcttttctga gtgttgatga ggtgcttgac
     7321 acatacccgg actggcatga ctcaagcttc tggccagaaa gcccgacgca cgctgttaag
     7381 agacagcgcg aagctaaaaa acaaggtgac ccgcttagta aaaaaggact tattggagcc
     7441 ttttgtcgta actatgacat tagacaggcc attgcaacgt tcttacctga ggtttatgaa
     7501 gaaggaacaa ctcctgatag gtacacctac actgagggct caaccgcaaa cggcttagtt
     7561 atctatgatg atgtcttcgc ttatagtcat cacgggacag atcccgtggg ggatacgctt
     7621 gtaaatgcat acgaccttgt tcgtatccat aaattcggag agcaagatag cggggctaaa
     7681 gataatactc ctactaataa gctaccatca agcaaagcga tgaatgcttt tgtctgtgac
     7741 ttacccgaaa ttaaagacta tctaatggcg gaggctttag gcgatttcga tgaagagtta
     7801 ccagtcgaag atgacagaag ctggcttgaa attgatgaga ggggtgaacc tgaggtcaat
     7861 agttatttgc tagcaacgca gattattaag gaggttccga tttattggga cggctttgag
     7921 ttcttaagat acgacgctaa aaaaggcatc tggttgccaa atgcagagga atatttgaag
     7981 agttatatct caactaagaa actcggtaaa attactaaga ttaggcacat tagcgaaacc
     8041 attgtagcga ttaaagcaca ggctttttca agcgaagtgt ttaccgagag tgatcttaac
     8101 aagatagtgc tagcgaacgg agtctatgac ctgagggata acagctttaa gactaagttt
     8161 gatccagaat tgcatgccag gtcaagccat cccgttgtct atgaccccga ggcgacctgt
     8221 gaaacctttg agggttttct tagggagacc gtcggagctg aaaatataga tttcatcttt
     8281 gagtggtttg gctataactt ttatcgtgaa tacactattc aaaaaatgct attcatctat
     8341 ggcagcggcg gtacgggtaa atcaacactg attaatattt tacgtgaaat gataggtgct
     8401 gataattatt cagccgtgac actgcagtac ctgatgcaag aacgctttgc aaaaatcggc
     8461 ttatatcgta agacagccaa ctttgacact gacgcaaagc cccaatattt agcagatggc
     8521 gcaaccctta aaatgttgac gggagaggat actatccacg cagaccgtaa aaataaagag
     8581 ccgattaact tttacaatta tgctaagctg tcttttgcca tgaatgagct cccgcctatg
     8641 cgagatttca gcggaggact taagcgccgc atgatgatcc ttgagatgga taaagtttta
     8701 acgcaggaag ttaaggcaaa atacccccta gataagatta tgggcgaagt gtctggtatc
     8761 tttaacagag cgatggaggg gcttagaaag gccttaagta agagagattt cagtattagt
     8821 gccagcatga ggtcaagtgt cgagaaatgg gaaaaaggca acgatgtcgt agctatgttc
     8881 cttgaagacg agtgtgaacc tggtgaagac ttcaaagctc ctgttaggga cgtctaccca
     8941 gcctataaat tctattgtca ggattcaggc tacaaacctt tggcaaaaaa tgcatttaac
     9001 caccggctaa gagagctaaa ttatgaaaat aaaaacgtta aatctggtgg aaagcaagcc
     9061 aaaaattggg tcggttttag gctaaaaagt gagttttagg ggttactttt ttgggattag
     9121 agtttggtaa ccctagaaaa aagttaccaa gttaccggtt tttcaaactt taaaaataaa
     9181 aaatttaaaa ataattttta aaaaaccaaa actgaattgg aaaaatcggt aacttagtaa
     9241 ctcgaccgtt agaaatgtgt tcgaaaagta accccggtag ctttttggta gccctaagag
     9301 gcttttccta gcaatagttt agcccctatg g
//
LOCUS       DATROD010000073         9243 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.073, whole genome shotgun sequence.
ACCESSION   DATROD010000073 DATROD010000000
VERSION     DATROD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9243
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.073"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..809)
                     /locus_tag="VBC74_001485"
     CDS             complement(<1..809)
                     /locus_tag="VBC74_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEO6942423.1"
                     /translation="MIRFLILAGYFELSMYLKLSGKLNQYINTHYTYLAYISMVLSFI
                     LAIVQLIIWVKNMKMHSHLHGKIAKSTSPMILVFPVLVGLLVPTVSLDSTTVSAKGYN
                     FPLAAGSTGTVSQDGTRVQYLKPDTSTYFTSSAYEKEMQKELKKYKGSGTLTITTENY
                     MEVMELIYLYPEQFMDRQIQYTGFVYNEPKHEGYQFIFRFGIIHCIADSGVYGLLTTG
                     NQKSYPDNTWVTVRGTIKSEYNQLLQQNLPVLHIEESRQVSKPNNPYVYRV"
     gene            complement(809..1711)
                     /locus_tag="VBC74_001486"
     CDS             complement(809..1711)
                     /locus_tag="VBC74_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEO6942424.1"
                     /translation="MDIFNQLPDSVLQWFAIFISIIIEALPFVLLGTILSGIIEVFIT
                     PDIVNKFLPKNKFLRVLFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSIRFLILRFVGATIVAIALGVMLAFLVDDNILKEDAKPTHFH
                     DYSDKKWYQKIFLALAHAIDEFFDTGRYLVFGTLIASAMQIYLPTRVLTTIGHSPITA
                     ILVMMLLAFILSLCSEADAFIGASLLSTFGIAPVMAFLLIGPMIDIKNLMMMVNSFKT
                     RFIVQFISVSSLIIIIYCLFVGVI"
     gene            complement(1717..1845)
                     /locus_tag="VBC74_001487"
     CDS             complement(1717..1845)
                     /locus_tag="VBC74_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6942425.1"
                     /translation="MAITHKRQDDLESMFASFAKVPKPKKVDSDSKPEQKDDSQKD"
     gene            1978..3021
                     /locus_tag="VBC74_001488"
     CDS             1978..3021
                     /locus_tag="VBC74_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEO6942426.1"
                     /translation="MIELGITTFGETTILEETNQSYSHPERIRQLVAEIELADQVGLD
                     VYGIGEHHREDFAVSAPEIILAAGAVRTNNIRLSSAVTILSSNDPIRVYQQFSTIDAL
                     SNGRAEIMAGRGSFIESFPLFGYDLADYDDLFNEKMDMLLAINSATNLDWKGHLTQTV
                     NERPIYPRALQRQLSIWVATGGNVDSTIRIAEQGLPIVYATIGGNPKAFRQLVHIYKE
                     VGSRNGHKPEQLKVAAHSWGWIEEDNQTAIDRYFFPTKQTVDNIAKGRPHWSEMTKEQ
                     YLRSVGPEGAIFVGSPEVVAHKIIGLVEALELDRFMLHLPVGSMPHKDVLNAIKLYGE
                     EVAPIVRKYFEKN"
     gene            3125..5287
                     /locus_tag="VBC74_001489"
     CDS             3125..5287
                     /locus_tag="VBC74_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEO6942427.1"
                     /translation="MENLNITQIAIDLGIKASQIEKVLELTDEGNTIPFIARYRKEMT
                     GNLDEVQIKSIIDLDKSMTALSDRKTTVLAKIEEQGKLTQELKKAIEEATKLADVEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNKDNLEEEAQNYLTDGFETTTKALSGAVD
                     ILIEAFSEDNKLRSWTYNEIWNYSSITAVVKDESLDEKQVFKIYYDFSEKISKLHGYQ
                     VLALNRGEKMGVLKVNFEHNLEKMFRFFAVRFKETSQYIDDLIVQTVKKKIVPAMERR
                     IRTELSEGAEDGAISLFSENLRNLLLVSPLKGKMVLGFDPAFRTGAKLAVVDQTGKLM
                     TTQVIYPVPPANQAKIEQSKIELAKLIKEFNIEIIAIGNGTASRESEAFVAEVLQDFP
                     DVSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLAENLDFVVETVVNQVGVNVNTASPALLAHVSGLNKTISENIVKYR
                     EENGQIKSRAEIKKVPRLGAKAFEQAAGFLRIPNAKNFLDNTGVHPESYEAVKKLLDQ
                     LTIKELDDLAKEKLQHLDLIATAESIGVGQETLKDIIEDLLKPGRDLRDDFEAPVLRH
                     DVLDVSDLKVGQELQGTVRNVVDFGAFVDIGVHEDGLIHQSRLIKRKRDKKTRKMPPL
                     PHPSKYLSVGDIVTVWVVEVDAERGRIGLSLIKPDGLD"
     gene            5250..5708
                     /locus_tag="VBC74_001490"
     CDS             5250..5708
                     /locus_tag="VBC74_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEO6942428.1"
                     /translation="MDLVLSSQTDLTNYVKKVSIEDFGCQFLHTAHWNNRLRTTGGRF
                     FPNDRHLDFNPKIYREFGIDIFRKIVRHELCHYHLYIQGKGYRHRDKAFKELLEKVDG
                     LRYTPRVTAVKVNYHHYSCQSCGQVYRRRRRVNLRKFACGYCHGRLIESF"
     gene            5789..6052
                     /locus_tag="VBC74_001491"
     CDS             5789..6052
                     /locus_tag="VBC74_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEO6942429.1"
                     /translation="MKSSFYKQRKGKLVCGVVAGLADKYNWDLALSRVLIALILYFTK
                     FGLLLYILLAVFLPYKEDIIETRRQGPRRRKDAEPVDDDGWFW"
     gene            6280..7215
                     /locus_tag="VBC74_001492"
     CDS             6280..7215
                     /locus_tag="VBC74_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEO6942430.1"
                     /translation="MAVTVQMLVDRLKLNVIYGDEHLLSKRITTADISRPGLEMTGYF
                     DYYAPERLQLVGMKEWSYLMAMTGHNRYQVLREMFQKETPAIVVARDLEIPEEMYEAA
                     KDTGIAILQSKAPTSRLSGEVSWYLDSCLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDIMSLYGAS
                     AVKDSSQVQLAIYLENFETGKVFDRLGNGNEEIELSGVKVPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKQMGFDATQTFEDRLTHLISQNEVNDD"
     gene            7208..7981
                     /locus_tag="VBC74_001493"
     CDS             7208..7981
                     /locus_tag="VBC74_001493"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEO6942431.1"
                     /translation="MINPVAIQLGPFSIRWYAICIVSGMLLAVYLAMKEAPRKNIKSD
                     DILDFILMAFPLSIVGARIYYVIFEWAYYSKHPVEIIAIWNGGIAIYGGLITGAILLV
                     IFSYRRLINPIDFLDIAAPGVMIAQAIGRWGNFINQEAYGRAVKNLNYLPNFIKNQMY
                     IDGAYRVPTFLYESLWNFLGFVIIMSIRHRPRTLKQGEVACFYLVWYGCGRFIIEGMR
                     TDSLYLAGLRVSQWLSVILVIIGIVMIIYHRREQHISYY"
     gene            7996..8394
                     /locus_tag="VBC74_001494"
     CDS             7996..8394
                     /locus_tag="VBC74_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEO6942432.1"
                     /translation="MIEIAVLIIAIAFVVLVLGILFVLKKVSETIEETKQTIKVLTSD
                     VNVTLYQTNEILAKANVLVDDVNGKVSTIDPLFVAIADLSESVSDLNLQARHIGQKAS
                     SATSSVTKAGSALAIGKAASKIFRKKGDKK"
     gene            8391..8822
                     /locus_tag="VBC74_001495"
     CDS             8391..8822
                     /locus_tag="VBC74_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEO6942433.1"
                     /translation="MSKLFKTLVISAASGAAAAYFLTTKKGKELRKNAEKFYGEYKEN
                     PEEYHQIAKDKASEYSNLAVDTFKDYKGKFESGELTTEDIVSAVKEKSGEVVDFANDF
                     VNQAKSKFSDEDTAKKEDKAPETKVEDIVIDYKENTEDKEK"
     gene            complement(8863..9138)
                     /locus_tag="VBC74_001496"
     CDS             complement(8863..9138)
                     /locus_tag="VBC74_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEO6942434.1"
                     /translation="MSSPLKQEQTHESKTEQAQPKFQVFQEFDHSAKLRELVFFARIA
                     LFSITTVLISFLLLALSLTPVWSFLLAMLASLAVTSAVSSFIWSLRQ"
BASE COUNT         3085 a         1432 c         1858 g         2868 t
ORIGIN      
        1 aagacacgat aaacataagg attattaggt ttagaaactt gacgagactc ttcaatgtgt
       61 aagactggta agttttgttg taacaattga ttatactcgg atttaattgt acctctaaca
      121 gttacccaag tattgtccgg ataacttttt tgatttccag ttgttagtag accataaact
      181 cctgaatcag caatacaatg aatgattcca aagcggaaaa taaattgata tccttcgtgt
      241 ttgggttcgt tataaacaaa cccagtatat tgtatttggc gatccatgaa ttgctcggga
      301 tagagataaa ttagttccat aacttccatg tagttttcag ttgtgatggt taatgtacca
      361 gagcctttat attttttaag ctctttttgc atttcttttt cataggctga agaagtaaaa
      421 taagtgctgg tatctggttt caaatattga acgcgtgtcc catcttgact gacagtacca
      481 gtagagccgg ctgctagagg gaaattatat cccttggcag agactgtggt cgagtctaaa
      541 ctaactgtag gtactaacaa acctactaaa acgggaaata ctaaaatcat tgggcttgtt
      601 gatttagcta ttttaccatg taggtgacta tgcattttca tatttttaac ccaaataatt
      661 agttgtacaa ttgctaaaat aaaagatagg accattgaaa tataggccaa ataggtataa
      721 tgagtattaa tatactgatt tagtttacct gataacttta aatacatgct taattcaaaa
      781 taaccagcaa gaataagaaa tcgaatcatt atattacccc cacaaataaa caatagataa
      841 taataataag agaagataca ctaatgaatt ggacaataaa ccttgtttta aaagaattta
      901 ccatcatcat taaattctta atatcaatca tgggtccaat taaaaggaaa gccatcactg
      961 gcgcgattcc aaaggttgat aataaagaag cacctataaa agcgtcagct tcactacaca
     1021 atgacaagat gaaagcaagc aacatcataa ctaagatggc tgtgataggg ctgtgtccga
     1081 tagtagtaag aactcgagtt ggtaaataaa tttgcatagc tgaagcaatt aaagtgccaa
     1141 atactagata gcgtcctgtg tcaaaaaatt catcgatggc atgagctaag gctagaaaga
     1201 ttttttgata ccacttctta tcagaataat catgaaaatg agtaggttta gcgtcttctt
     1261 ttagaatatt atcatctact aaaaaggcaa gcatgactcc taaagcaatg gctacaatgg
     1321 tagcacctac aaagcgtaat atcaggaagc gtatagaatt tccaaatgct gagtaggtcg
     1381 caaataaaac aataggatta ataataggag cagtagcaag aaacggaact gccgtgtatg
     1441 aaggcacttt cttttcaagg aatcgattaa taataggaat gataccacat tcgcaagaag
     1501 gaaagacaaa gccaacgaag gttccaaata agactctgag aaatttattt ttcggtaaga
     1561 acttattgac tatatcaggt gttataaaga cttcaataat tcctgataaa atagttccaa
     1621 gcaaaacaaa aggaagtgcc tcaataataa tggaaatgaa aatggcaaac cattgaagga
     1681 cactatccgg taattgatta aagatatcca tagaacttaa tctttctgag aatcatcttt
     1741 ctgttcaggt tttgaatcag aatcaacttt tttaggtttt ggtactttag caaaactagc
     1801 gaacatactt tctaagtcat cttgacgttt gtgcgtaata gccattaatg gcacctcttt
     1861 ctttatataa taacctatta tactatatta gcttattttt aacataaaaa aacaaatcga
     1921 ttggaaatga tactatatag tgataaaatg ttattagtag attgatgagg taagattatg
     1981 attgaattag gtattacaac attcggagag acaacaatcc ttgaagaaac aaaccaaagc
     2041 tattcacatc ctgagaggat tcgccaatta gttgctgaga ttgaactagc tgatcaagtt
     2101 ggtttagatg tatatggtat tggagagcac catcgtgaag attttgcggt ctctgcaccc
     2161 gaaattatcc tagcagcagg agcggttaga actaataata tccgtttatc tagtgcagta
     2221 acgattctct cttccaatga tcctattcgc gtctatcagc aattttcaac gattgacgca
     2281 ctttcaaatg gtagagcaga aattatggca gggcgtggtt cctttattga gtcttttcca
     2341 ttgtttggat acgatttagc ggattatgat gatttattta atgaaaaaat ggatatgttg
     2401 ttagcaatta actcagcgac aaatctcgat tggaaaggtc atttgacaca aacagttaat
     2461 gagcgaccaa tttatccaag agcattacaa agacagttat caatatgggt ggcaacagga
     2521 ggaaatgttg attctacaat tcgtattgca gaacaaggtt tgccaattgt ttatgcaact
     2581 attggtggga atcccaaagc ctttcgtcaa ttggtccata tttataaaga agttggtagt
     2641 cgtaatggac acaaaccaga acaactaaaa gttgctgctc actcttgggg atggattgaa
     2701 gaggataatc aaaccgctat tgaccgttat tttttcccta cgaaacagac cgtcgataat
     2761 attgctaagg gacgccctca ttggtctgaa atgactaaag agcagtattt acgttcagta
     2821 ggtccagaag gtgctatttt tgtaggaagt cctgaagtgg ttgcacataa aattatagga
     2881 cttgttgaag cgcttgagtt agaccgtttt atgttacatc taccagttgg ctctatgcct
     2941 cacaaagacg tcctaaatgc catcaaactt tatggtgagg aagtagcacc aattgttcga
     3001 aaatattttg aaaagaatta aagcagacct ggtctgtttt ttattgttag attataaaaa
     3061 cgctatcttg aaatcgcttt ttaaaactct tcgtaagtca aaaatatgct ataataaaag
     3121 cgttatggaa aatttaaata ttacacaaat tgctattgat ttaggtatta aagcgtcaca
     3181 aatcgaaaag gttttagaat taacagatga aggaaatact attcctttta ttgcccgtta
     3241 tcgtaaagaa atgacaggta atttggatga agttcaaatc aaatcaatta ttgaccttga
     3301 taagagtatg acagctcttt cagatcgtaa aactacggtc ttagccaaaa ttgaagaaca
     3361 aggaaagttg actcaagaac tcaaaaaggc tattgaagaa gcaactaaac tagcagatgt
     3421 tgaggaactc tacctacctt ataaagaaaa acgtcgtacg aaagctacga ttgcacgtga
     3481 agcaggtctt tttcctttag cacgtcttat tttacaaaat aaggataacc ttgaggaaga
     3541 agctcaaaac tatcttacag atggttttga gacaactacg aaggcccttt ctggagcagt
     3601 agatattctt atcgaagctt tttctgagga taataaatta cgttcatgga catataatga
     3661 gatctggaac tacagctcaa tcactgcagt tgttaaagat gagtcacttg atgaaaaaca
     3721 ggtcttcaaa atctactatg acttttctga aaaaataagt aaactccatg gctatcaagt
     3781 tttagcattg aatcgtggtg agaaaatggg tgttcttaag gttaattttg agcataacct
     3841 tgaaaaaatg tttcgctttt ttgctgttcg atttaaagaa actagccaat atatagatga
     3901 ccttattgta cagactgtca agaaaaaaat tgttccagct atggaacgcc gtatccgtac
     3961 agaactaagt gaaggagcag aagatggtgc aatttcactg ttttcagaga atcttcgtaa
     4021 cttattgtta gtctcaccgc ttaaggggaa aatggtgtta ggttttgacc ctgcctttcg
     4081 tacaggagca aaattagcag tagtagacca aactggaaaa ttaatgacaa cacaagtgat
     4141 ttatccagtt cctccagcta atcaagcaaa aatcgaacaa tcaaaaatag agttagcaaa
     4201 acttatcaaa gaattcaata tagaaattat tgctataggg aatggaacag cgagtcgtga
     4261 atcagaagca tttgttgcag aagttttaca agattttcca gacgtatcct atgtgattgt
     4321 taacgaaagt ggtgcctctg tttattcagc ctcagaattg gcacgtcatg aatttccaga
     4381 cttaacagtt gaaaaacgtt cggctatctc aattgcaaga cgtctgcaag acccgttagc
     4441 agaactagtc aaaattgatc caaaatctat tggtgtgggt caatatcaac atgatgttag
     4501 tcaaaaaaaa ttagcagaaa atcttgattt tgttgttgaa actgtagtta accaagtagg
     4561 tgttaatgtt aatacagcaa gtccagcctt gttagctcac gtttcaggtt taaataaaac
     4621 aatatctgaa aatattgtta aatatcgtga agagaatggg caaattaagt cacgtgctga
     4681 aattaagaaa gtacccagat taggagcaaa agcttttgag caagctgctg gattcttacg
     4741 tattcctaat gctaaaaatt tcttggataa tacaggcgtt caccctgagt cttatgaagc
     4801 tgttaaaaag ttgcttgacc aactgactat caaagaatta gatgatcttg caaaagagaa
     4861 gttacaacac cttgacttaa ttgcaacagc agaaagtatt ggggtaggtc aagaaacatt
     4921 aaaagacatt atagaagatc ttttgaaacc aggtcgtgat ttgcgtgatg attttgaagc
     4981 gcctgttttg agacatgatg ttttagatgt cagtgattta aaagttggac aggaattgca
     5041 aggaactgtt cgaaacgtcg ttgattttgg tgcttttgtt gatattggtg ttcatgaaga
     5101 tggtctaatc caccagtctc gccttattaa gcgaaaacgc gataaaaaaa ctagaaaaat
     5161 gcctccttta ccacatccaa gcaaatatct ctctgttggt gatattgtaa ctgtttgggt
     5221 tgtagaggta gatgcggagc gtggcagaat tggacttagt cttatcaagc cagacggact
     5281 tgactaatta tgttaagaag gtgtctatag aagattttgg atgtcagttt ttacatactg
     5341 cacattggaa taatcgctta agaacaaccg gaggacgctt ctttccaaac gatagacacc
     5401 ttgactttaa tcccaaaatt tatcgtgagt ttggtattga tatttttcgt aagattgtaa
     5461 gacatgagct ttgccattat catctctata tacaaggtaa aggatatcga catagggata
     5521 aagcttttaa agagttacta gaaaaagtcg atggcttacg atatacccct agagttacgg
     5581 ctgttaaagt gaattatcat cattactctt gccaatcttg tggtcaagta taccgacgta
     5641 ggagacgtgt taatttacgc aagtttgctt gtggttattg tcatggtcgc ttgatagaga
     5701 gtttttaaaa tcagtcgtaa ggcctattta attttactgt caaagtagtt ataattaaaa
     5761 tatagagtag atagaaagaa ggataggtat gaaatcaagc ttctacaaac aacgaaaagg
     5821 taagttggtc tgcggtgttg tcgccggtct agctgataag tataactggg atttagctct
     5881 ttcacgggtg ttgattgcac tgattttgta ttttaccaaa tttggtcttt tgctatatat
     5941 tttattagct gttttcttac cctataaaga agatattatt gaaacaagaa gacaaggtcc
     6001 cagaagacgt aaagatgctg aacctgttga tgatgacggt tggttctggt aattcatata
     6061 aaggagattt gctagacgaa tctccttttt tcttagcttt atttagacca ttttttcaaa
     6121 atatcttctt tttttcagaa aaacgcgtta gcgcttactt ttagtaaaaa gtatgatata
     6181 atgtgggtgt aaattaatga aagtgtttag tacctttgct caactttaaa ttgtagggta
     6241 tagcaatagg gatattcact tttataagaa ggagtaagta tggcagttac tgttcaaatg
     6301 ttagttgacc gcttaaagtt aaatgtcatc tatggtgatg aacatctatt atctaagcgg
     6361 attacaacag ctgacatttc acgtcctggt ctagaaatga cagggtattt tgattattat
     6421 gcaccagagc gtttgcaact tgttggtatg aaagagtggt catatttaat ggctatgaca
     6481 ggacataatc gttatcaagt gttacgtgag atgttccaaa aagaaacacc agctattgtt
     6541 gtagcacgtg atttagaaat tccagaagag atgtatgagg cggctaaaga taccggtatt
     6601 gctattcttc aaagtaaggc ccctacaagt cgtttatcag gtgaagtttc ttggtattta
     6661 gattcttgct tagctgagag gacaagtgtt catggtgtct tgatggatat ctatggtatg
     6721 ggtgttctga ttcaaggaga ttccggtatt ggtaagagtg agacaggact agagctagtt
     6781 aagcgaggac atcgtttggt agcagatgac cgtgttgatg tttatgctaa agacgaagag
     6841 acgctttggg gagagcctgc tgagatatta cgtcatttac ttgaaattag aggtgtggga
     6901 atcattgata ttatgagcct atatggagct agtgcggtta aagattcatc gcaagtgcaa
     6961 ttagctatat accttgaaaa ctttgaaact ggaaaagttt ttgatagact cggaaatggc
     7021 aatgaagaga tcgaattatc tggagttaag gttcctcgga tacgtattcc tgttaaaaca
     7081 ggccgaaacg tgtcagtagt tattgaagca gcggctatga atcacagagc taaacaaatg
     7141 gggtttgatg ctacccagac ttttgaggat cgtttaacac atttgattag tcagaacgag
     7201 gtcaatgatg attaatccag tagcaataca attaggcccc ttttctattc gctggtatgc
     7261 tatttgcata gtgtctggta tgttgttagc agtttactta gcaatgaaag aggcccctcg
     7321 taagaacatt aagtcggatg atattctaga tttcattctc atggcttttc cactatccat
     7381 tgttggagct cgtatttatt atgttatctt cgaatgggct tattactcta agcacccagt
     7441 agagattatc gccatttgga acggtggtat tgctatttac ggagggttaa ttaccggagc
     7501 cattctcctt gtgatttttt catatcgtcg cttgattaat cccattgatt ttctggatat
     7561 tgctgctcca ggagtgatga ttgcccaagc cataggtaga tgggggaatt ttattaatca
     7621 agaagcatac ggtagggcag taaaaaatct gaattactta ccaaacttta taaaaaatca
     7681 aatgtatatt gatggagctt accgagtacc tacattttta tatgagtctc tttggaactt
     7741 cctaggtttt gtcatcatca tgtctattcg tcatcgaccg cgcactttaa aacaaggtga
     7801 agtggcatgt ttttatttag tttggtatgg atgtggacgg ttcattattg aagggatgag
     7861 gacagatagc ctttatttgg cgggtttgcg cgtgtcacaa tggttatcag ttattttagt
     7921 tattatcgga attgtcatga ttatttatca tcgtcgtgaa caacatattt catattatta
     7981 aacagaggag gcattatgat agaaattgct gtattaatta ttgccattgc gtttgttgtt
     8041 ttagttttag gaatactttt cgttctgaaa aaagtatcag aaacgattga ggaaaccaag
     8101 caaactatta aagtattaac aagcgatgtt aacgtgactc tttaccaaac taatgaaatt
     8161 ttagccaaag ctaatgtttt agttgatgat gttaatggta aagtatctac tattgatcct
     8221 ctttttgtag ctattgctga cttatcagaa tctgtatctg atttaaacct acaggcaaga
     8281 cacattggac aaaaggcaag tagtgcaacc tcaagtgtta ctaaggcagg gtcagcttta
     8341 gctattggaa aagcggcttc aaaaattttt cgtaaaaaag gagacaaaaa atgagtaaat
     8401 tatttaaaac tttagttatc agtgctgcat caggtgcagc agctgcttat tttttaacaa
     8461 caaaaaaagg aaaagagcta aggaaaaatg cagaaaaatt ctatggagaa tataaagaaa
     8521 atccagaaga atatcatcaa atagctaaag ataaagcaag tgaatattca aatttagctg
     8581 ttgatacttt taaagattat aaaggtaaat ttgaatcagg tgaattgaca acagaggata
     8641 tcgtctcagc cgttaaggaa aaaagcggag aagtagttga ctttgctaat gattttgtca
     8701 atcaagctaa atcaaaattc tcagacgagg atactgctaa aaaagaagat aaggctcctg
     8761 aaacaaaagt agaagatatt gtcattgatt ataaagaaaa cacagaagat aaagaaaaat
     8821 aaaaaagaag ctactataag tagcttcttt tttatgagat ttttattgtc tgagtgacca
     8881 aatgaatgaa gagactgcag atgtaactgc tagacttgcc agcatcgcta gtaagaaact
     8941 ccacacaggt gttaaactta gtgctagtaa taagaaagag attagcacag tggttatact
     9001 gaagagagcg atacgagcaa aaaagactag ctcacgcaac ttagcactgt gatcaaattc
     9061 ttgaaatact tgaaattttg gttgagcttg ctctgttttg ctttcgtgag tttgctcttg
     9121 cttaagtggt gatgacatag ctttctcctt tgatactagc atatcatatc acaaataata
     9181 gagtttgtca ctagtttttt gatactatga gttaaaataa aggttttttc tcaaaaaaac
     9241 aaa
//
LOCUS       DATROD010000074         8443 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.074, whole genome shotgun sequence.
ACCESSION   DATROD010000074 DATROD010000000
VERSION     DATROD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8443
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.074"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..746)
                     /locus_tag="VBC74_001497"
     CDS             complement(<1..746)
                     /locus_tag="VBC74_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter (permease)"
                     /protein_id="HEO6942435.1"
                     /translation="MFGKLLKYELKSVGKWYLTLNAAVLLVSIILGLVLKALGGNFST
                     DTNSTSAQIFTIILVLLLAMVISGSLLSTLAIIIKRFYSNIFGRQGYLTLTLPVTTNQ
                     IICSKLLASLLWSIFNIFIVIIGIILVILPLVGIGQFVVAFPEIYKIISSSNAPLFIA
                     YFFLSYVAGTLLIYLSIAVGQLFTNKRVLMGIVSYFGISLLITFLTLIIDSIFHIDLF
                     NSHANATFSQPVLLYNILVSIVEIAIFYMLT"
     gene            complement(758..1456)
                     /locus_tag="VBC74_001498"
     CDS             complement(758..1456)
                     /locus_tag="VBC74_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6942436.1"
                     /translation="MTQLLQLHHVTKKYHKHIAVNDVTVSIPTGKIIGLLGPNGSGKT
                     TIIKMINGLLQPDKGDIVIDGYRPSVETKKIISYLPDTSYLQENMKIKDVVTLFEDFY
                     NDFNSKVAYQLFEDLNLNPKERLKNLSKGNKEKVQLILVMSRKARLYILDEPIGGVDP
                     AARDYILKTIISNYSNDASVLISTHLISDIEPILDEVIFLKEGEIDLQGNADDLREEH
                     NCSIDALFRERFKK"
     gene            complement(1457..1825)
                     /locus_tag="VBC74_001499"
     CDS             complement(1457..1825)
                     /locus_tag="VBC74_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6942437.1"
                     /translation="MAWEFNEKSPIYSQIAEHIKMQIVSQEIKSGDQLPTVRELAQEA
                     GVNPNTMQRAFTELEREGMVFSQRTSGRFVTEDNLLIGKIRQQVAKAELATFVNNMKK
                     IGYKLDEITVALDHFIKEIN"
     gene            1970..5074
                     /locus_tag="VBC74_001500"
     CDS             1970..5074
                     /locus_tag="VBC74_001500"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEO6942438.1"
                     /translation="MFAQLDTRTVYSFMDSLVDLKTYVSKSKSLGYQTIGILDHSNLY
                     AAYHFIQEAQKANLRPIVGFSFDIVVENRSIEVYCIAINTVGYKNLLKLSTAQMSEKM
                     SLNLLTEHLEGVQLILPYQDALDQLNLPFDYVIGVNLTSPQIPYTKPIIAIDTVRYFQ
                     KNDIETLQMLHAIRDNVLLKDAQYASKNQELKPCQEMTLAFKNRFPEALANLESLVEN
                     VVYHFDSDFKLPIFNREIPAGEELRTLTQNNLKSKGLWSDAYQERLEKELTIIHKMGF
                     DDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVSYALNITGIDPVKHNLIFERFLNEE
                     RYSMPDIDIDLPDIYRGEFLRYVRNRYGSMHSAQIVTFSTFGAKQAIRDVFKRFGASE
                     YELTNITKKIHFRDNLTSVYNRNLAFRQIIDSKIEYQKAYDIAKRIEGNPRQTSIHAA
                     GVVMSDDLLTDHIPLKNGEDMMITQYDASSVEANGLLKMDFLGLRNLTFVQKMKEKVD
                     KDYGISIQLEAIDLEDKETLKLFAAGQTKGIFQFEQSGAINLLRRIRPECFEDVVATT
                     SLNRPGASDYTENFINRRFGKEKIDLVDPVIAPILQPTYGIMLYQEQVMQIAQTYAGF
                     TLGKSDLLRRAMSKKNSKEMQKMSQSFLEGAVSKGHRQEDARLIFERMAKFAGYGFNR
                     SHAFAYSALAFQLAYFKAHYSDVFYDIMINYSNSDYLIDAIDFGFVIEKPSINTISYR
                     DRIYKKKIYLGLKNIKAVPNDLAYWISKNQPFQSIEDFLMRLPQQFQKSGFISPLIAI
                     GAFDEFDNNRRKITSNLDSLFTFVNELGSLFADTSYHWLEVEDFSNSEKYEMEQDILG
                     VGISPHPLVGISQKASRPFIPISQVQENSEATILVQLKQVKIIRTKSSGQQMAFLTVM
                     DINSKMDITVFPETFNIVRDDLQEGKYYYLHGKIQKRDERLQMVLNGVQEATEERFWI
                     LLKNHDNDKKISEILSKYKGHIPVYLHYETTKETIQSKVHLVRKDSGLALDLSEFVVK
                     TVYQ"
     gene            5155..6177
                     /gene="pfkA"
                     /locus_tag="VBC74_001501"
     CDS             5155..6177
                     /gene="pfkA"
                     /locus_tag="VBC74_001501"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEO6942439.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINQGYYGM
                     VTGDIFPLDANSVGDTINRGGTFLRSARYPEFAELEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGLPGTIDNDIVGTDYTIGFDTAVATAVENLDRLRDTSASH
                     NRTFVVEVMGRNAGDIALWSGIAAGADQIIVPEEEFNIDEVVSNVRAGYAAGKHHQII
                     VLAEGVMSGDEFAKTMKAAGDDSDLRVTNLGHLLRGGSPTARDRVLASRMGAYAVQLL
                     KEGRGGLAVGVHNEEMVESPILGLAEEGALFSLTDEGKIVVNNPHEADLRLAALNRDL
                     ANQSSK"
     gene            6226..7728
                     /gene="pyk"
                     /locus_tag="VBC74_001502"
     CDS             6226..7728
                     /gene="pyk"
                     /locus_tag="VBC74_001502"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEO6942440.1"
                     /translation="MNKRVKIVATLGPAVEFRGGKKFGESGYWGESLDVEASAEKIAQ
                     LIKEGANVFRFNFSHGDHAEQGARMATVRKAEEIAGQKVGFLLDTKGPEIRTELFEDG
                     ADFHSYTTGTKLRVATKQGIKSTPEVIALNVAGGLDIFDDVEVGKQILVDDGKLGLTV
                     FAKDKDTREFEVVVENDGLIGKQKGVNIPYTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVNEVRAICEETGNGHVKLFAKIENQQGIDNIDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIITKVNAAGKAVITATNMLETMTDKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDKNAQTLLNEYGRLDSSAFPRNNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRSLMINWGVIPVLAD
                     KPASTDDMFEVAERVALEAGLVESGDNIVIVAGVPVGTGGTNTMRVRTVK"
     gene            7899..>8443
                     /gene="lepB"
                     /locus_tag="VBC74_001503"
     CDS             7899..>8443
                     /gene="lepB"
                     /locus_tag="VBC74_001503"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001931829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEO6942441.1"
                     /translation="MVKRDFIRNIILALIAVLILILLRYFVFATFKVHKDATNSYFSN
                     GDVVVVNRNRTPKYKDFIVYKVGKIFYISRVIGEPNQKVRVMDDILYLNDVFKDEPYI
                     EKMKNAYSEKKDGQMPFTSDFSVETLTRNKESRVPKGSYLVLNDNRQNKNDSRKFGLI
                     KEKDIRGVITFKVYPLSEFGFT"
BASE COUNT         2741 a         1316 c         1656 g         2730 t
ORIGIN      
        1 gtcaacatat agaatatagc aatttcaaca atgctaacaa gtatattgta gagcaataca
       61 ggttgagaga atgttgcatt tgcatgagag ttgaataaat caatatgaaa aatactatct
      121 attattaatg ttaagaaagt aattaagagt gatattccaa aataagaaac aattcccatc
      181 aatacacgtt tgtttgtaaa taattgccct acagcgatag agagataaat cagtaatgtc
      241 cctgcaacgt acgatagaaa gaaataggcg ataaaaagtg gagcgttcga agatgaaata
      301 attttataga tttctggaaa agcgacaacg aactgaccaa tacctactaa aggtaatatg
      361 accaatatga ttccaataat gacaataaaa atattaaaaa tactccaaag aagcgaagct
      421 agtagctttg aacaaataat ctgattcgta gttactggga gcgttaaggt tagatatcct
      481 tgacggccga agatattgct ataaaaacgt ttgataataa tagcaagggt ggataacaga
      541 gatcctgaaa taaccatcgc taacaataaa acgagtatta tagtgaatat ttgggcgcta
      601 gtactattag tatcagtaga aaaattgccg ccaagtgcct ttaataccag tcctaaaata
      661 atagatacta ataaaaccgc agcgttgagt gttagatacc acttaccaac tgattttaat
      721 tcatatttta ataattttcc aaacatatca atctccttta tttcttgaat ctttcacgaa
      781 agagagcatc aatcgaacag ttgtgctctt ctcttaagtc atcagcattg ccttgtaggt
      841 caatttctcc ttctttaagg aagataactt catcaaggat aggttcaatg tcagaaatta
      901 agtgagtcga aattaaaaca gaagcatcat tagagtagtt gctaatgatt gtttttaaaa
      961 tgtagtcacg cgctgcaggg tctacaccac caataggttc atcaaggatg tagagcctag
     1021 ctttacgact cataacaagt attagttgaa ctttctcctt gttccctttt gataggtttt
     1081 ttagacgttc cttaggattt aggttaagat cctcaaatag ttgataagct acctttgaat
     1141 taaaatcgtt atagaaatct tcaaataatg taacgacgtc tttgattttc atattttctt
     1201 gtaaatatga agtatcaggg agatatgaaa taattttttt ggtttcaaca gacggtctgt
     1261 aaccatcgat aacaatatct cccttatctg gctgaagtaa tccatttatc attttgatga
     1321 ttgttgtttt tccgctacca tttggaccta ataaacctat aatctttcct gttggaatgc
     1381 ttacagtcac atcattaaca gcaatatgtt tgtgatattt ctttgtaaca tgatgaagtt
     1441 gtaataactg tgtcatttaa ttaatctcct ttataaaatg atcgagagca accgtaattt
     1501 catctaattt atatcctatt tttttcatgt tgttgacaaa agttgctaac tctgcttttg
     1561 caacttgttg acgaatttta ccaattagta agttgtcttc tgtaacaaag cgtccggaag
     1621 tacgttgtga gaataccatt ccttcacgtt ccaattcagt gaaggcacgt tgcatggtat
     1681 ttgggttaac accagcttct tgagctaact ctctcacggt aggcaattgg tcaccacttt
     1741 ttatttcttg actaacaatt tgcatcttta tatgttcagc aatttgtgaa taaataggtg
     1801 atttttcatt gaattcccaa gccatagata actcctttca taatcttgta ctttttgtac
     1861 tataaaaata gtacaaaaaa atatttgaaa tgtcaaggaa ttatcattga gactttaatc
     1921 gtttattgtt tcatagtata atagtaaaaa atcgtaaaaa ggggctacta tgtttgcaca
     1981 acttgataca agaaccgttt attcttttat ggatagcttg gttgatttaa agacatatgt
     2041 ctctaaatcg aaatcattag gatatcaaac tattggtatc ttggaccaca gtaatcttta
     2101 tgcagcatat cattttattc aagaggcgca aaaggcaaac ctaagaccaa tagttggatt
     2161 tagctttgat atagtggttg aaaatagatc aatagaagtt tactgtattg cgataaatac
     2221 agttggttat aagaatctct tgaagttgtc aacggctcaa atgtctgaga agatgtctct
     2281 aaatttattg acagaacatc ttgaaggggt gcaattgatt cttccctatc aagatgctct
     2341 tgatcagctc aatttaccct ttgattatgt tattggtgtt aatttgacct ctccgcagat
     2401 tccatatacc aaacctatta tagccataga tactgttcga tattttcaaa aaaatgatat
     2461 tgagacatta cagatgcttc atgctataag agataatgtc ctgttaaaag atgcacaata
     2521 tgcgagcaaa aatcaagaat taaaaccttg tcaagaaatg actctagcat tcaagaaccg
     2581 ttttcctgaa gctttagcta acctagaatc tttggtagag aatgttgttt atcactttga
     2641 ttctgacttt aagttaccta tttttaatag agaaattcct gctggagaag aattaagaac
     2701 cttaactcaa aataacttaa aaagtaaagg tttatggagc gatgcttatc aagagcgtct
     2761 cgaaaaagaa ttaacaatca ttcacaaaat gggctttgat gactatttcc ttattgtgtg
     2821 ggatttatta agatttggtc gtagtaaggg ctactatatg ggaatgggac gtggttcagc
     2881 agcgggaagt ttagtctcat atgctttaaa tattaccggt atcgatcctg tcaaacataa
     2941 tttaattttt gaacgttttt taaatgaaga gcgttattcg atgccagata ttgatattga
     3001 tcttccggat atttatcgtg gagaattctt gcgctatgta cgtaatcgat atggttcaat
     3061 gcattctgca cagattgtaa ccttctcaac ttttggtgcg aaacaggcta ttcgagatgt
     3121 ttttaaacgc tttggtgcat cagagtatga gttaacaaac ataactaaaa aaattcattt
     3181 cagagataat ttgaccagtg tttataaccg caacttagct ttcagacaaa taatagatag
     3241 taaaattgaa taccaaaaag cctatgatat tgctaagcgt attgaaggaa atcctagaca
     3301 gacttctatt catgccgctg gggtagtaat gagtgacgat ttgttaacag atcatattcc
     3361 tttaaaaaac ggtgaggata tgatgataac gcaatatgat gctagttctg ttgaggctaa
     3421 tggtcttcta aaaatggact ttctaggtct tcgaaatttg acatttgttc agaaaatgaa
     3481 ggagaaagtc gataaggact acggtatctc tatacaatta gaagctattg atttagaaga
     3541 taaagagact ctaaaactat ttgcggctgg tcaaacaaag gggatttttc aatttgagca
     3601 aagtggagct attaatttgt tgcgacgtat taggccagag tgttttgaag atgtggtagc
     3661 tactaccagc ttgaataggc ctggtgcaag tgattatact gagaatttta taaaccgtcg
     3721 ttttggtaaa gaaaaaatcg atttagtaga tcctgtcata gctccaattt tacaacctac
     3781 ctatggcata atgctctatc aagaacaggt tatgcagatt gcccagacct acgcaggttt
     3841 tacgctaggc aagtctgatt tgctcagacg tgctatgtct aagaaaaatt ctaaggagat
     3901 gcaaaagatg tctcaatcct tcttagaggg tgcagtatct aagggacatc gtcaagaaga
     3961 tgctcgactt atatttgaac gcatggctaa atttgcaggt tatgggttta atcgcagcca
     4021 tgcctttgct tattcggctt tagcttttca attagcctat ttcaaagccc attattccga
     4081 tgtcttttat gacatcatga taaattattc aaatagtgat tatctaatcg atgctattga
     4141 ttttggtttt gttattgaaa aaccttcaat taatactatt tcatacagag accgcatcta
     4201 taaaaagaaa atttatttag gtttaaaaaa tattaaggct gttcctaacg acttggctta
     4261 ttggatttct aaaaatcaac catttcagag tattgaagat tttctaatga gactgccaca
     4321 gcagtttcaa aaaagtggtt ttatttctcc gctaattgct attggagcat ttgacgaatt
     4381 tgataacaat agacggaaaa tcacctctaa tcttgattct ttatttacat ttgtaaatga
     4441 actaggtagt ttatttgcag atacctcata tcattggtta gaagtagaag atttctctaa
     4501 ttctgagaaa tatgaaatgg aacaagatat cttaggagta ggaatcagtc cgcacccttt
     4561 agtggggatt tcacaaaaag ctagccgtcc gtttatacca attagtcaag tccaagaaaa
     4621 tagcgaagct actattttag ttcagctaaa gcaggtcaaa atcattagga caaaaagttc
     4681 tggacaacaa atggctttct tgactgttat ggatattaat tcaaaaatgg atattactgt
     4741 ttttcctgaa actttcaata ttgtaagaga tgatttacaa gaagggaaat attattactt
     4801 gcatggaaaa attcagaagc gagatgagcg attacagatg gttttaaacg gtgttcaaga
     4861 agcaacggag gaacgttttt ggatacttct aaaaaatcat gataacgaca aaaaaatatc
     4921 ggaaatattg tccaaatata aagggcatat tcctgtctat ttgcattatg aaacaactaa
     4981 agaaactatc caaagcaaag tacatttggt tagaaaagat agtggcttag cccttgattt
     5041 atctgaattt gttgtgaaaa cggtttatca ataaaattgc atataaagtg cagaaattta
     5101 caaattaata tgctataatt aaatcgttaa attaataaaa ggagtaccaa ttaaatgaaa
     5161 cgtattgctg ttttaactag tggtggtgac gcccctggta tgaacgctgc tatccgtgca
     5221 gttgttcgta aagcaatttc tgaaggtatg gaagtttacg gcatcaacca aggttactat
     5281 ggtatggtga caggggatat tttccctttg gatgctaatt ctgttgggga tactatcaac
     5341 cgtggaggaa cgtttttacg ttcagcccgt tatcctgaat ttgctgaact tgaaggtcag
     5401 cttaaaggga ttgaacagct taaaaaacac ggtattgaag gtgtagtagt tatcggtggt
     5461 gatggttctt atcatggtgc tatgcgtcta actgagcacg gtttcccagc tgttggtttg
     5521 ccgggtacaa ttgataacga tatcgttggc actgactata ctattggttt tgacacagca
     5581 gttgcgacag cagttgagaa tcttgaccgt cttcgtgata catcagcaag tcataaccgt
     5641 acttttgttg ttgaggttat gggaagaaat gcaggagata tcgctctttg gtcaggtatc
     5701 gctgcaggtg cagatcaaat tattgttcct gaagaagagt tcaatattga tgaagttgtc
     5761 tcaaatgtta gagctggcta tgcagctggt aaacatcacc aaatcatcgt tcttgcagaa
     5821 ggtgttatga gtggtgatga gtttgcaaaa acaatgaaag cagcaggaga cgatagcgat
     5881 cttcgtgtga cgaatttagg acatctgctc cgtggtggta gtccgacggc tcgtgatcgt
     5941 gtcttagcat ctcgtatggg agcgtacgct gttcaattgt tgaaagaagg tcgtggtggt
     6001 ttagccgttg gtgtccacaa cgaagaaatg gttgaaagtc caattttagg tttagcagaa
     6061 gaaggtgctt tgttcagctt gactgatgaa ggaaaaatcg ttgttaataa tccgcatgaa
     6121 gcggatcttc gcttggcagc acttaatcgt gaccttgcca accaaagtag taaataagaa
     6181 tttgtcaatt tgacacttta aataaaaagg agctcataaa ttattatgaa taaacgcgta
     6241 aaaatcgttg caacacttgg tcctgcggta gaattccgtg gtggtaagaa gtttggtgag
     6301 tctggatact ggggtgaaag ccttgacgta gaagcttcag cagaaaaaat tgctcaattg
     6361 attaaagaag gtgctaacgt tttccgtttc aacttctcac atggagatca tgctgagcaa
     6421 ggagctcgta tggctactgt tcgtaaagca gaagagattg caggacaaaa agttggcttc
     6481 ctccttgata ctaaaggacc tgaaattcgt acagaacttt ttgaagatgg tgcagatttc
     6541 cattcatata caacaggtac aaaattacgt gttgctacta agcaaggtat caaatcaact
     6601 ccagaagtga ttgcattgaa tgttgctggt ggacttgaca tctttgatga cgttgaagtt
     6661 ggtaagcaaa tccttgttga tgatggtaaa ctaggtctta ctgtgtttgc aaaagataaa
     6721 gacactcgtg aatttgaagt agttgttgag aatgatggcc ttattggtaa acaaaaaggt
     6781 gtaaacatcc cttatactaa aattcctttc ccagcacttg cagaacgcga taatgctgat
     6841 atccgttttg gacttgagca aggacttaac tttattgcta tctcatttgt acgtactgct
     6901 aaagatgtta atgaagttcg tgctatttgt gaagaaactg gcaatggaca cgttaagttg
     6961 tttgctaaaa ttgaaaatca acaaggtatc gataatattg atgagattat cgaagcagca
     7021 gatggtatta tgattgctcg tggtgatatg ggtatcgaag ttccatttga aatggttcca
     7081 gtttaccaaa aaatgatcat tactaaagtt aatgcagctg gtaaagcagt tattacagca
     7141 acaaatatgc ttgaaacaat gactgataaa ccacgtgcga ctcgttcaga agtatctgat
     7201 gtcttcaatg ctgttattga tggtactgat gctacaatgc tttcaggtga gtcagctaat
     7261 ggtaaatacc cagttgagtc agttcgtaca atggctacta ttgataaaaa tgctcaaaca
     7321 ttactcaatg agtatggtcg cttagactca tctgcattcc cacgtaataa caaaactgat
     7381 gttattgcat ctgcggttaa agatgcaaca cactcaatgg atatcaaact tgttgtaaca
     7441 attactgaaa caggtaatac agctcgtgcc atttctaaat tccgtccaga tgcagacatt
     7501 ttggctgtta catttgatga aaaagtacaa cgttcattga tgattaactg gggtgttatc
     7561 cctgtccttg cagacaaacc agcatctaca gatgatatgt ttgaggttgc agaacgtgta
     7621 gcacttgaag caggacttgt tgaatcaggc gataatatcg ttatcgttgc aggtgttcct
     7681 gtaggtacag gtggaactaa cacaatgcgt gttcgtactg ttaaataatt tttgaattca
     7741 aacctattat ttaagaatac cttaaataat gggtttttta tatgttttgc tttttattat
     7801 tcgattattg tgactagaat aggatatgat attaataatt accctccttt ttttatctat
     7861 gaaaacatga tataatggaa aaaagattag gagtagttat ggttaaacgc gattttatta
     7921 gaaatattat tttagcattg attgctgtat tgattttgat tctcttgaga tattttgttt
     7981 ttgcaacctt taaagtacat aaggatgcta caaattctta ttttagcaac ggagatgttg
     8041 ttgttgttaa tcgtaatagg acgcctaagt acaaagactt tattgtctat aaggttggaa
     8101 agattttcta tattagtcgt gttattggtg agccaaatca aaaagtgaga gtaatggatg
     8161 atattttata tttgaatgat gttttcaagg atgagcctta cattgaaaaa atgaaaaatg
     8221 cttattctga aaagaaagac ggtcaaatgc cctttacttc cgacttctca gttgaaacat
     8281 taacacgaaa taaagaaagc agagttccta agggttctta tcttgtgtta aatgataatc
     8341 gacaaaataa aaatgatagt cgaaaatttg gattaatcaa agagaaagat attcgtggtg
     8401 ttattacctt taaagtatac cctctaagtg aatttggttt tac
//
LOCUS       DATROD010000075         8442 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.075, whole genome shotgun sequence.
ACCESSION   DATROD010000075 DATROD010000000
VERSION     DATROD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.075"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..547
                     /locus_tag="VBC74_001504"
     CDS             <1..547
                     /locus_tag="VBC74_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6942442.1"
                     /translation="SHISGRTISSPLTTFALDLIKNKVYKEKDFVTNYKAIVSPLNKE
                     VRGSTFFIKNAQNELEGMLCINLDISAYQNIALDILDLVNLNVNKILSKSPQKISLPQ
                     QEEPVEVLSGNIQDIISEIVDPSLLNQNIHLSQEVKVEIVSKLHEKGVFQLKGAVSKV
                     AEVLNISEPSVYRYLKKIETH"
     gene            717..2969
                     /gene="gshAB"
                     /locus_tag="VBC74_001505"
     CDS             717..2969
                     /gene="gshAB"
                     /locus_tag="VBC74_001505"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEO6942443.1"
                     /translation="MIIDQLLQRSHSHLPILQATFGLERESLRIHQPTQRVAQTPHPK
                     TLGSRNYHPYIQTDYSEPQLELITPIAKDSQEAIRFLKAISDVAGRSINHDEYLWPLS
                     MPPKVREEDIQIAQLEDAFEYDYRKYLEKTYGKLIQSISGIHYNLGLGQELLTSLFEL
                     SQADNAIDFQNQLYMKLSQNFLRYRWLLTYLYGASPVAEEDFLNQKLNNPVRSLRNSH
                     LGYVNHKDIRISYTSLKDYVNDLENAVKSGQLIAEKEFYSPVRLRGSKSCRNYLEKGI
                     TYLEFRTFDLNPFSPIGITQETVDTVHLFLLALLWIDSSSHIDQDIKEANRLNDLIAL
                     SHPLEKLPNQAPVSDLVDAMQSVIQHFNLPPYYQDLLESVKRQIQSPKLTVAGQLLEM
                     IEGLSLETFGQRQGQIYHDYAWEAPYALKGYETMELSTQLLLFDVIQKGVNFEVLDEQ
                     DQFLKLWHNSHIEYVKNGNMTSKDNYIVPLAMANKVVTKKILDEKHFPTPFGDEFTDR
                     KEALNYFSQIQDKPIVVKPKSTNFGLGISIFKTSANLASYEKAIDIAFAEDSAILVEE
                     YIEGTEYRFFVLEGDCIAVLLRVAANVVGDGIHTISQLVKLKNQNPLRGYDHRSPLEV
                     IELGEVEQLMLEQQGYTVNSIPPEGTKIELRRNSNISTGGDSIDVTNTMDPTYKQLAA
                     EMAEAMGAWVCGVDLIIPNATQAYSKDKKNATCIELNFNPLMYMHTYCQEGPGQSITP
                     RILAKLFPEL"
     gene            3093..3911
                     /locus_tag="VBC74_001506"
     CDS             3093..3911
                     /locus_tag="VBC74_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942444.1"
                     /translation="MVKKRYSKNSHNLITLLGIVVLASLISDFWSEVIATLLIIGGGY
                     CAYYVYDKKRLKCFTSNQRIEALKSDIKETDQDIRHLEILKKDNRSKEYIKLAHQILP
                     QLDLIRNEANQLQKAIEPNIYKRITKKANTFSNEINEQLIKLHASPELEPISDQEDEM
                     IRIAPELKPFYHNIQDDHYAILKKIEEADNKAELAAIHQANMKRFTDVLAGYIRIKQS
                     PKNFNNAKERLEQALQAIKKFNLDLDETLRQLNESDMKDFDVSLRMMQDERNNK"
     gene            3908..5164
                     /locus_tag="VBC74_001507"
     CDS             3908..5164
                     /locus_tag="VBC74_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEO6942445.1"
                     /translation="MSDTFNFDIDQIADNAITKTDKTTEIISNQTTSQTGQIAFFEKL
                     TPAQKSAISEKTPALVDTFVGDQNALLDFGQSAVEGVNTTVNHILSEQKKIQIPQVDD
                     LLKNANRELNGFIAKYKDATPAELEKKPNLIQKLFKQSKTSLQEFYFDSQNIEQKMDM
                     MAANVVKQEDTLARNIVSAEMLIEDNTKSIENLVGVIAFIESSQAEAANRASHLQQEI
                     LALDSQTSEYQIKSNQLARMTEVINTLEQQHTEYVSRLYVAWATTPQMRNLVKVSSDM
                     RQKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGVTADAIVNANNAALQMLAETSKEAIP
                     MLEKTAQSPTVSIKSVTALAESLVAQNNGIIAAIDKGRKERAQLESAVIKSAETINDS
                     VKIRDKKIVEALLNEGKSTQEKVDES"
     gene            5304..6179
                     /gene="hslO"
                     /locus_tag="VBC74_001508"
     CDS             5304..6179
                     /gene="hslO"
                     /locus_tag="VBC74_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEO6942446.1"
                     /translation="MDKIIKSISTSGSFRAYVLDCTETVRTAQEKHQTLSSSTVALGR
                     TLIANQILAANQKGNSKVTVKVIGDSSFGHIISVADTKGNVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVVITDYGTGQPYTSTTPLITGEIGEDFAYYLTESEQTPSAVGLNV
                     LLDDEDKVKVAGGFMLQVLPGASDEEISRYEKRIQEMPSISSLLESENHIESLLSAIY
                     GEDDYKRLSEDSLAFYCDCSKERFEAALLTLGTKELQAMKDEDKGVEITCQFCNQTYY
                     FTEEDLEKIINDSIK"
     gene            6163..7140
                     /gene="dusB"
                     /locus_tag="VBC74_001509"
     CDS             6163..7140
                     /gene="dusB"
                     /locus_tag="VBC74_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEO6942447.1"
                     /translation="MTQLNSSFMIGKVEIPHRTVLAPMAGITNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQNNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPEKIYHIVKEVTSVLDIPLTVKMRTGWSDSSNAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLGKVAKAVTSIPFIANGDIRTVHDAK
                     FMIEEIGADAIMVGRGARSNPYIFTQINHFFETGEILPDLPFEKMLDVAEDHLTRLVN
                     LKGETIAVREFRGLAPHYLRGKSGAAKIRGAVSRAETLAEVQELFAGLR"
     gene            7261..7902
                     /locus_tag="VBC74_001510"
     CDS             7261..7902
                     /locus_tag="VBC74_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEO6942448.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGQHLGTDVFYEAVDNNPVLDLYYQ
                     DPKKYAFLLQIFFLNKRFQSIKEAYKANNNVLDRSIFEDELFLTLNFKNGNVTKTELD
                     IYKELLANMLEELEGMPKKRPDLLVYIDVSFDKMLERIDKRGRSFEQVDSNPELYDYY
                     KQVHSEYPEWYENYDVSPKIRIDGNKLDFVKNPEDLQHVLDTIDSELQKLDLL"
     gene            7916..8407
                     /locus_tag="VBC74_001511"
     CDS             7916..8407
                     /locus_tag="VBC74_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEO6942449.1"
                     /translation="MIIRPVLPSDAKELLAIYAPYVTDTTITFEYDIPSISEFTNRIK
                     NISKSFPYLVAEENGKILGYAYASTYYARASYDWTCELSIYLDKDVRSKKIGSQLYDT
                     LEKELVNQGYVNLLACISLPNDISIAFHKKRGFNQVAHFPKIGFKFEQWHDIVWLQKR
                     LKS"
BASE COUNT         2899 a         1528 c         1485 g         2530 t
ORIGIN      
        1 tagtcacatt agtggacgaa ccataagttc acctctaacg actttcgccc ttgatttaat
       61 caagaataaa gtttataagg aaaaagattt tgttactaac tataaggcga ttgtcagccc
      121 tctcaataaa gaagttcgtg gatctacttt cttcatcaag aatgctcaaa atgaacttga
      181 agggatgtta tgtatcaacc ttgatatttc tgcataccaa aatattgctt tggatattct
      241 agacttagtt aatttaaatg ttaacaaaat actatctaag tcccctcaaa aaatttctct
      301 ccctcaacag gaggaacctg tagaagttct atcaggcaat attcaggata ttattagtga
      361 gattgttgat cctagtcttt taaatcagaa tattcattta agtcaagaag ttaaggtaga
      421 aattgtttct aaacttcacg aaaaaggtgt attccaatta aaaggtgccg tttcaaaggt
      481 cgctgaagtt ctcaacatct ccgaaccaag cgtctatcgt tatcttaaaa aaattgaaac
      541 tcattaactc taaagctctt tttaacttat agattaataa gattggactc aaaagttcaa
      601 tcttttattt tttatgacac cagtataaga ttattttagt tttctgtgcc gtaaaactta
      661 gtttttatta tttttttgat aaactagtat catctgttac taggaggaat gttgtcgtga
      721 ttatcgatca actgttacaa agaagccact ctcatctacc gattctacaa gctacatttg
      781 gcttagagag agaaagcctt cgtattcacc aacccactca aagggttgct caaacacccc
      841 atcccaaaac attagggagt cgtaactatc atccttatat ccaaacggat tatagtgaac
      901 ctcaattaga actcattaca cctattgcaa aggatagcca agaagccatt cgatttttga
      961 aagctataag tgatgtcgct ggacgatcta tcaaccacga tgaatactta tggccgcttt
     1021 ctatgccacc caaagtgagg gaagaagaca tacagattgc ccaattagag gatgcttttg
     1081 aatatgatta tcgcaaatat ttggagaaaa cttatggaaa actaatacaa tccatctctg
     1141 gaattcacta taatctcggt ttaggtcaag agttattaac ttctctcttt gaattgagtc
     1201 aagcagataa tgctattgat tttcagaacc agctttacat gaaattgtct caaaattttt
     1261 tacgttaccg ttggttacta acttatctat acggagcgag cccagtagct gaagaagact
     1321 ttttaaatca gaaacttaat aaccctgtcc ggtcacttag aaatagtcac ttaggctatg
     1381 tgaatcataa agatattcgt atttcttata ctagcttgaa ggattatgtc aacgatctag
     1441 aaaatgctgt aaaaagtggg caattgattg ctgagaaaga attttattca cctgttcgtc
     1501 tccgtggtag taagtcctgt cgtaattatt tagaaaaagg aattacatat ttagaatttc
     1561 gcacttttga tctcaaccca tttagcccaa tcggtataac gcaggaaact gttgatactg
     1621 ttcacctttt cttattggcg cttttgtgga tagattctag tagtcatatt gaccaagata
     1681 tcaaggaagc caatagatta aatgacctta tagcactcag tcatccatta gaaaaattac
     1741 ctaatcaagc accagtttct gacttagtgg atgctatgca atctgttatc caacatttta
     1801 acttaccacc ttactatcaa gacctattgg aatctgtaaa aaggcaaatt cagtcaccta
     1861 aattaacggt agctggtcaa cttttagaaa tgattgaagg actttcctta gaaacatttg
     1921 gacaaagaca aggacaaatt tatcacgatt atgcttggga agctccctac gccttaaagg
     1981 gatatgaaac gatggaactt tctacccaat tgctactatt tgacgttatc caaaaaggag
     2041 tcaacttcga agtactggat gaacaagatc aattcctaaa attatggcac aatagtcata
     2101 ttgagtacgt taaaaatggt aatatgacct caaaagataa ctatattgta ccgctcgcta
     2161 tggctaacaa agttgttaca aaaaaaatcc tagatgaaaa gcatttccca actccttttg
     2221 gagatgaatt tactgaccgt aaagaggcac ttaactattt ttcacaaatc caagataaac
     2281 caatcgtcgt taagccaaaa tctacaaact ttggtttagg catttctatt tttaaaacat
     2341 cagctaactt agcttcttat gaaaaagcaa ttgacattgc ttttgccgaa gatagcgcta
     2401 ttcttgtgga agaatatatt gagggtaccg aataccgttt cttcgtcctt gagggggact
     2461 gtattgctgt attgcttagg gtagcagcta atgtcgttgg agatggtatt catactatta
     2521 gtcagctagt taaacttaaa aaccaaaacc ctctcagagg ctatgatcat cgctctcctc
     2581 tagaggttat tgagctcgga gaagtagaac agttaatgtt ggaacaacaa ggatacactg
     2641 ttaacagtat ccctccagaa ggaacaaaaa tagaacttcg ccgtaattct aatatttcca
     2701 ctggcggtga ctctatagat gtaacaaata caatggatcc tacatataaa caactagcag
     2761 ctgagatggc agaggctatg ggggcttggg tttgtggagt tgatttaatt attcctaatg
     2821 ccactcaagc atactcaaaa gataagaaaa atgctacttg cattgaacta aactttaacc
     2881 cattgatgta tatgcacacc tactgtcaag aggggcctgg tcaatctatt acacctcgaa
     2941 ttttggctaa actgttccca gaattataac taaaaagagt tccttaggct aagcttaagg
     3001 aatttttggt taaaataaag ttatcccttg tcatcactag ttcatttaag ataaaatatt
     3061 aacaactaca gaaagttagg ttaaatatta ttatggttaa aaaacgctat tccaaaaatt
     3121 ctcataatct aataacatta cttggtattg ttgttcttgc ttccctgatt tcagactttt
     3181 ggagtgaggt gattgctact ctgttaatta ttggtggtgg ttattgtgcc tattacgttt
     3241 atgataaaaa gcgtcttaaa tgttttacat caaaccaacg tattgaagct ttaaaatccg
     3301 atattaaaga gactgaccaa gacattagac acttggaaat tttaaaaaaa gacaatcgtt
     3361 ctaaggaata tatcaaatta gcacatcaaa tattacctca attggaccta attcgtaatg
     3421 aagcaaacca actacaaaaa gccattgagc ctaatatcta taaacgtatc accaaaaaag
     3481 caaatacatt tagtaatgag attaacgaac aattaataaa gctacatgcc tctccagaac
     3541 tagaacctat ttctgatcag gaagatgaaa tgatacgcat tgctccggaa ttaaaaccgt
     3601 tctatcacaa tattcaagat gatcactatg ctatcttaaa aaagattgaa gaggcagata
     3661 acaaagctga attagcagca attcatcaag ccaatatgaa acgttttaca gatgttctgg
     3721 ctggctacat cagaatcaaa caatctccta aaaattttaa caatgctaag gaacgcctag
     3781 aacaagcatt acaagcaatt aaaaaattta atttagattt agatgaaacg ctgcgccaat
     3841 tgaatgaaag tgatatgaaa gattttgacg ttagcctaag aatgatgcaa gacgaaagga
     3901 acaataaatg agcgatacct ttaattttga tattgaccaa attgcagaca atgctatcac
     3961 taaaacagat aaaacaacag aaattatttc caaccagaca acaagccaaa ctgggcaaat
     4021 tgcctttttt gaaaaactaa caccagcaca aaagtctgct atctctgaaa aaacaccagc
     4081 tttggtagat acttttgtcg gtgaccaaaa tgcgctcctt gattttggac aatccgcagt
     4141 agaaggcgtt aatactactg ttaatcatat cttgtctgag cagaaaaaaa ttcaaattcc
     4201 tcaagttgat gatttactaa aaaatgctaa tcgcgaacta aatggattta ttgccaaata
     4261 taaagatgct actccggcag aattagagaa aaaaccaaac ttgattcaaa aattattcaa
     4321 acaaagcaag acctcgctac aggaatttta ttttgactca caaaacatcg agcaaaaaat
     4381 ggatatgatg gcagcaaatg ttgtcaaaca agaagatact ttggcaagaa atatcgtctc
     4441 tgctgaaatg ctcattgaag ataatactaa atctattgaa aatttggttg gagttattgc
     4501 ttttattgaa tcgagtcaag ccgaggctgc caatcgtgca agccacttac aacaagaaat
     4561 tctagcatta gatagccaaa cgtccgaata tcaaattaaa agtaaccaat tagcccgaat
     4621 gactgaagtt atcaataccc tcgaacagca acatacggaa tatgtcagcc gtctctacgt
     4681 tgcatgggca acaacaccac agatgcgaaa cttggtcaaa gtatcgtcag atatgcgtca
     4741 gaaacttggt atgttacgtc gaaataccat tccaacaatg aaactctcaa tcgctcagtt
     4801 aggcatgatg caacaatctg tcaaatccgg tgtcactgct gatgctattg tcaacgctaa
     4861 taatgcagca ttgcaaatgc tggctgaaac tagtaaagaa gcgattccga tgttagagaa
     4921 gaccgcacaa agccccactg tttctattaa atctgtcact gcattagctg aaagcttagt
     4981 ggctcaaaat aatggtatta tcgctgccat agacaaagga cgtaaggaac gtgcccaatt
     5041 agaatctgct gttattaaat cggctgaaac aatcaatgat tctgtcaaaa ttcgtgataa
     5101 aaaaatagtt gaagccttac tcaacgaagg taaatctacc caagaaaaag ttgatgagtc
     5161 ttaacttact atcatatcca ctatataaaa acgatactgt aaaacacagt atcgttttta
     5221 tatcattaat ttataccgtt ttcatctata ttttcctgac tatctccaat gcaaaataag
     5281 ccttttatgc tataatatga cttatggata aaattataaa atcaatttca acttccggtt
     5341 ctttccgggc ctatgtcctt gattgtacag aaactgtaag aacagctcaa gaaaaacatc
     5401 agacactttc aagttctact gtagctcttg ggagaacatt aattgcaaat caaattttag
     5461 cagcaaatca aaaaggaaat agtaaagtaa ctgtcaaggt tattggagat agctcttttg
     5521 gtcatattat ttctgtagct gatactaaag gaaatgttaa aggttacatc caaaatacgg
     5581 gtgttgatat caagaaaaca gctactggag aggtcttagt tggacctttc atggggaatg
     5641 gacactttgt cgttattacc gattacggaa ctgggcaacc ttacacttca acaactccac
     5701 taattacagg agaaattgga gaagattttg cttattattt aaccgaaagt gagcaaacac
     5761 cttctgccgt cggtttaaat gtcctcttag atgacgagga taaggttaaa gttgcaggtg
     5821 gattcatgct tcaggtgcta ccaggtgctt ctgacgaaga aatttctcgc tatgaaaaac
     5881 gcattcagga gatgccttct atttcaagtc tgctagagtc tgaaaatcat atagaatctc
     5941 tcctttcagc tatttacgga gaagacgact acaagcgctt atcggaagac tctttagcct
     6001 tctattgtga ctgttcaaaa gaacgttttg aagccgctct attgacttta ggaactaaag
     6061 aattgcaagc catgaaggat gaagataaag gtgttgaaat tacttgtcaa ttttgcaacc
     6121 agacttacta tttcacagaa gaagatttgg agaagataat taatgactca attaaatagt
     6181 tcatttatga ttgggaaggt tgaaattcct catcgtaccg tactagcacc gatggcaggt
     6241 attactaatt ctgcttttcg tactatcgct aaagaatttg gtgctggtct cgttgttatg
     6301 gaaatgattt ctgagaaagg gttactttat aacaacgaaa agaccttaca tatgctccat
     6361 atcgatgaaa atgaacatcc tatgtcaatt caactatttg gtggcgatgc agaaggctta
     6421 aaacgtgcag ctgactttat ccaaaataat acgaaagcag atatcgtaga catcaatatg
     6481 ggatgtccag ttaacaaagt tgttaaaaac gaagctggag ctaaatggtt acgtgaccct
     6541 gaaaaaattt atcatattgt taaagaggta acgtctgtac ttgacattcc attaacagta
     6601 aaaatgcgta caggatggtc tgactcatct aatgctgtag aaaatgcact cgctgccgaa
     6661 tctgctggtg tatctgcact tgctatgcac ggtcgaacac gtgaacaaat gtatactggc
     6721 acgtgtgacc atgaaacact tggcaaggtc gctaaagctg ttactagtat tccttttatt
     6781 gccaatggtg acattcgtac tgttcacgat gccaaattta tgattgaaga gattggagct
     6841 gacgctataa tggttggacg tggtgctcgt agcaatcctt acatctttac acaaattaat
     6901 catttctttg aaacaggcga aatccttccc gacctaccat ttgagaagat gttagatgtt
     6961 gccgaggatc atctcactcg actagttaat ttaaaaggtg aaactattgc cgttcgtgaa
     7021 tttcgagggt tagcacctca ttatctacgt ggaaaatcag gcgcagcaaa aattagagga
     7081 gctgtttcac gtgcagaaac actagcagaa gttcaagaac tttttgcagg tttacgctaa
     7141 atcaaaaaca gtcctttgac tgttttttcc tatctctgta caaatttagc ataaatcatt
     7201 gtgtaatgaa aactcaaaag tataacaaat tattttagta taacagaatt aaggagaatt
     7261 atgttgattg ttttagctgg aaccattgga gcaggtaaaa gctcccttgc agctgctcta
     7321 ggacaacacc tagggacaga tgttttctat gaggcggttg ataataaccc agttttagat
     7381 ctttactatc aagatcccaa aaaatatgct tttttactac aaatattctt tttaaataaa
     7441 cgattccaat ctataaaaga agcctataag gctaacaaca atgttttgga tcgttctatt
     7501 tttgaagatg aactattcct cactctaaat tttaagaatg gaaatgtgac taaaacagag
     7561 ctagatatct ataaagaact tcttgcaaat atgttggaag aattagaagg tatgcctaag
     7621 aagagacctg atttgctagt ctacattgat gtttcatttg ataaaatgtt agaacgtatt
     7681 gacaaacgag gacgcagctt tgagcaagta gattctaacc cagaattata cgactactac
     7741 aaacaagtcc actctgaata tccagaatgg tatgaaaatt acgacgtttc acctaaaatt
     7801 cggatcgatg gtaacaaact tgatttcgtc aaaaatccag aggatcttca acacgtcctt
     7861 gacactattg attccgaatt gcaaaaactt gatttactat aattaggtga tatctatgat
     7921 tataagacct gtcctaccct cagatgctaa agaattactt gctatctatg ctccctacgt
     7981 tactgacact acaataacgt ttgaatacga tattcctagt atatcggaat tcactaatcg
     8041 tatcaaaaat atcagcaaat ctttcccata tttagttgct gaagaaaatg ggaaaatatt
     8101 aggttatgca tatgcttcaa cttactacgc tagagcttcc tatgactgga cttgcgagct
     8161 ttctatttac cttgataaag atgtacgtag caaaaaaatt ggtagccaac tatatgatac
     8221 attggagaaa gaattagtta accaagggta tgttaatcta ctcgcttgta tcagtttacc
     8281 aaatgatata agcattgcat tccacaaaaa aagaggattt aatcaagtcg cacatttccc
     8341 aaaaatcggg tttaaatttg aacaatggca tgacattgta tggttacaaa agcgattgaa
     8401 atcttaaggt aatctttaga tttattttaa gttagcaaaa ga
//
LOCUS       DATROD010000076         8296 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.076, whole genome shotgun sequence.
ACCESSION   DATROD010000076 DATROD010000000
VERSION     DATROD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.076"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            135..1418
                     /locus_tag="VBC74_001512"
     CDS             135..1418
                     /locus_tag="VBC74_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HEO6942450.1"
                     /translation="MSLYYTEKAHAIAGPSDRQYVENPNPHIIVNVTGTDQNGNSILP
                     HYIEVNVKMGQTLSKEEILDYIARNLNSSVGGESKNVQYSNIEFKESAYLKRQLDDGK
                     TEEIAIDNDGVTVPKDGPNKFWIDVPVTCTVTPIVTETHEVRWGTPVAISHRIYFVEE
                     SSGKVLDEYTNLHTADSELNGYRVGDYITDYALSKSAYEAFLNSRLDKEGYKLQHRIS
                     TNVRQNLQINKLIFNYDFNEENIYYQIGNIRPLLSRSSAEVESDIITERYYVSKNAKS
                     LARTESTISIKMVDAKTEQPLFNHTLTGYQLVTVSHVYNRLFEENLIPTTKSGERYFI
                     QNMKKTAEQEYTVYLSETPYSKENAPVISYDARPVDWDYHSGASGSLENQPNIYTEED
                     STEFLGNKPQAACYPNKQFACENTDSKYNYSYLEK"
     gene            1573..1812
                     /locus_tag="VBC74_001513"
     CDS             1573..1812
                     /locus_tag="VBC74_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6942451.1"
                     /translation="MTLFLNSLLKELIIVNRLREYRIKASISQLALAKSVGVARQTIN
                     LIENNKYNPSLDLCIRLAECLQTDLNTLFWDSRHL"
     gene            1912..2445
                     /locus_tag="VBC74_001514"
     CDS             1912..2445
                     /locus_tag="VBC74_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEO6942452.1"
                     /translation="MKEKKNLGIKLIKSLYGINGPFDEYREQKINYLGNLLFATTSLY
                     HILSSIIAWFTVSKYPTETACTLLALTFFLYFCIFLIVIPSYLNSHDLITPLEVSKND
                     YPVAIKKNKQKFIKIGIFYTVIQWLILGLTTNNNTPLIVSLFSLKVILSSIMSGVAYN
                     GVMYLIFKNNVDIEKDE"
     gene            complement(2521..3015)
                     /gene="tpx"
                     /locus_tag="VBC74_001515"
     CDS             complement(2521..3015)
                     /gene="tpx"
                     /locus_tag="VBC74_001515"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEO6942453.1"
                     /translation="MTTFLGNPVTFTGKQLQVGDIAKDFLLIATDLSQKSLKDFEGKK
                     KVISVVPSIDTGICSKQTRTFNEELSELDNTVVITVSMDLPFAQKRWCSAKGLDNVIL
                     LSDFYDHSFGQEYALLMNEWHLLTRAVLILDEHNKVTYTEYVDNVNSDVDYEAAINAA
                     KILP"
     gene            complement(3088..3747)
                     /locus_tag="VBC74_001516"
     CDS             complement(3088..3747)
                     /locus_tag="VBC74_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEO6942454.1"
                     /translation="MDLFLSLERKFKAASDKEVSKQQETYLRHHFKCYGIKSPERRML
                     YKELIKAAKRQAKIDWQLLDKCWQSDYREYHHFVLDYLLAMSQFLTYNDCSRLEFYAR
                     HQQWWDSIDVLTKIFGNLSLKDDKVMNLLSEWSLDQDFWMRRLAIEHQLGFKEKTNTD
                     ILSLFILRNTGSQEFFINKAIGWALRDYSKYNKVWVKDFISNHYDELSTLSIREGSKY
                     L"
     gene            complement(3747..4511)
                     /locus_tag="VBC74_001517"
     CDS             complement(3747..4511)
                     /locus_tag="VBC74_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO6942455.1"
                     /translation="MDFVFDIDGTLCFDGMSLSKEIQGILERAQIDYGHRVTFATARS
                     YRDTIGILGDKLSLSKIIGLNGATLHENGHLVDSYYLQSDFFSTIISYCHRYQIPYFV
                     DEVFNYATYQASKIPFIAYVDPQKRGELLEVSKIEKPIKMVLYFGDQLRRADQMLAKL
                     NRFGLSSHFFHEFEKYLYINPIAVDKGKATKKLFGNRFIAFGNDKNDISMFDAAHYSV
                     QVGDFDELTPYANLRVSRESVHEGITMLFEKYKGIT"
     gene            complement(4516..5157)
                     /locus_tag="VBC74_001518"
     CDS             complement(4516..5157)
                     /locus_tag="VBC74_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6942456.1"
                     /translation="MVSEQSEIVTSKYQKIAVAVAQRIANGDYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLQILTLKHGSGAIILSKEKAIAFLNQYETSHSVAILKGKIR
                     DNIKAQQQEMEELATLVDDFLLQTRAVSKQYPLAPYEIIVSEDSEHLGKSIGELNVWH
                     QTGATIVAIEHEGKFIVSPGPFSVIEQGDHIFFVGDEDVYARMKTYFNLRMGL"
     gene            complement(5321..5872)
                     /locus_tag="VBC74_001519"
     CDS             complement(5321..5872)
                     /locus_tag="VBC74_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6942457.1"
                     /translation="MENQKQDIKTSVTYEEKVIAKIVGHALESVDGLLAVSGGFFSNL
                     KNSVVNSDSVTDGVNVEVGTKEVAVDLDIVVEYGKDIPAIVESIKAIVSQNVEVMTHL
                     KVVELNANVVDVKTKAEHEADSVTVQDRVSDAAQATGNFASEQAGKAKAAISSGAEKT
                     KEAVSNGTEAAKEKISEARTSES"
     gene            complement(5894..6091)
                     /locus_tag="VBC74_001520"
     CDS             complement(5894..6091)
                     /locus_tag="VBC74_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEO6942458.1"
                     /translation="MSEEKFDAKVDKVSGSVKESVGKLTGDKEVESEGKVDKLKGHAK
                     EKLADIKDTIKGASESFKKKD"
     gene            complement(6138..6680)
                     /locus_tag="VBC74_001521"
     CDS             complement(6138..6680)
                     /locus_tag="VBC74_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6942459.1"
                     /translation="MTETYIKNTTNNSGTTAVRGELTFEDKVIEKIVGIAIEHVDGLL
                     AVNGGFFSNLKNSVVNSDSVTDGVNVEVGKKQVAVDLDIVAEYQKHVPTIFADIKKVV
                     EAEVKRMTDLEVVEVNVNVVDIKTRAQHEEDSVTLQDRVTSAAQATGEFASNQVSNVK
                     SAVGNGVDKVEDMKSEPRVQ"
     gene            complement(6736..6930)
                     /locus_tag="VBC74_001522"
     CDS             complement(6736..6930)
                     /locus_tag="VBC74_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEO6942460.1"
                     /translation="MSEFVRKYRYPLGGAVIGLVLAAMIVTIGFFKTILALVIIVLGA
                     YAGLYVQRTGMLDQFFNKRK"
     gene            complement(6940..7521)
                     /gene="amaP"
                     /locus_tag="VBC74_001523"
     CDS             complement(6940..7521)
                     /gene="amaP"
                     /locus_tag="VBC74_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEO6942461.1"
                     /translation="MSKGLKSLYTLLGLISLTLLGFVAVISKQHIYLPSFNWLDWDFN
                     LPSPIDVGMYHYFFWGALVLFVIVLLAILVVLFYPRRYTEYKLADKTGKLMLKKSAIE
                     GFVKTEVLKTGLMKSPSVTAHLYKKKVKVDVKGLLTSRTNVPEQLEHIQSDVELGLKE
                     FFGLEKKMNTRVFVKQVEEENVGNAKTNKSRVE"
     gene            complement(7536..7784)
                     /locus_tag="VBC74_001524"
     CDS             complement(7536..7784)
                     /locus_tag="VBC74_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO6942462.1"
                     /translation="MSLIWSLIVGAIIGAIAGAVTNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLAGMALIPSIVGAIIVVIVTSFVLGKMNN"
     gene            complement(7832..8077)
                     /locus_tag="VBC74_001525"
     CDS             complement(7832..8077)
                     /locus_tag="VBC74_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEO6942463.1"
                     /translation="MGLIWSLIVGAIIGAIAGAITNKGGSMGWIANILAGLVGSFVGQ
                     SLLGTWGPKLADMALIPSIVGAIIVIIVTSFVLGKKS"
BASE COUNT         2773 a         1635 c         1160 g         2728 t
ORIGIN      
        1 attaattcct atcattcact gctttatttt aggataacta aacaatttat tgtttaaaat
       61 gaaaggattt atgaatgtct aggaaaacat ttaaacgtat actatcgatt ggagtttgca
      121 cgctcgtact atcgatgagt ctttattaca ctgaaaaagc ccacgctatt gctggtccta
      181 gtgaccgcca atacgtagaa aacccaaatc ctcacattat tgtaaatgtt acaggtactg
      241 atcaaaacgg aaatagcatt ttaccgcatt atatcgaagt caatgtaaag atgggacaaa
      301 ctttaagtaa agaagaaatt ctagattata ttgctcgaaa tttaaactct agtgttggag
      361 gagaaagtaa aaacgttcaa tacagcaaca tcgagtttaa ggaaagtgct tatctgaagc
      421 gtcaattaga tgatggcaag acagaagaaa tagcaattga taacgacggt gttactgtac
      481 ctaaagacgg tccaaacaaa ttttggattg acgttccagt aacttgtact gttactccta
      541 tcgtaacaga aacacatgaa gttcgatggg ggactccagt cgctatatca caccgtattt
      601 actttgttga agaatcttct ggaaaagttt tagatgaata cacaaatcta cacactgctg
      661 attcggaact taacggttat cgtgttggag attatatcac agactatgca ctttctaagt
      721 ctgcttacga agctttttta aactctcgtt tagataaaga aggttacaaa cttcaacatc
      781 gtattagcac gaatgtacga caaaaccttc aaattaataa attgattttc aattatgact
      841 ttaatgagga aaatatttac taccaaatcg ggaatatccg tccactatta agtcgctcat
      901 cagctgaagt agaatctgac atcattacag aacgctacta tgtttctaaa aatgctaaaa
      961 gtttagcacg tacagaatca accatttcga ttaaaatggt tgatgccaaa actgaacaac
     1021 cgctatttaa ccacacatta actggttatc aattggtaac tgtctcccat gtctataaca
     1081 gactctttga agaaaatctt atcccaacta caaaatcagg agaaagatat tttattcaaa
     1141 atatgaaaaa aacagctgaa caagaatata ctgtttatct ttcagaaaca ccttattcta
     1201 aagagaacgc tcccgtaatt tcttatgatg caagacctgt tgattgggat tatcactcag
     1261 gcgcttcagg atcacttgaa aatcagccta acatctatac tgaagaagat tcaactgaat
     1321 ttttgggtaa taaaccacaa gcagcttgtt atccaaacaa acaatttgct tgcgaaaata
     1381 ctgactctaa atacaactat agctatttag aaaaatagtt ataaccgata gggttaatct
     1441 ctcattatct tatactgatt aaaatcatta aacttgtttc cattttgact ggaaacaagt
     1501 tttttatatt tatttgtcac atataattga cactcttcga aaaatgatat atacttaaaa
     1561 tgtcatctat atatgacact tttcttaaat tctttactaa aggagctaat aatcgtgaat
     1621 cgtcttagag aatacagaat taaagctagc atatctcaat tagctttagc aaaatcagtt
     1681 ggtgtcgcca gacaaacaat aaaccttatt gaaaataata aatacaatcc ttcgctagat
     1741 ctttgtataa gactagcgga atgcctacaa acagacctca atactctttt ttgggatagt
     1801 agacatctat aacagattag actctctaaa tctatgaaaa ctcgggaggt taggtcgtat
     1861 tattatttca atctcaaact ttattaaata gttcaaggat aggagaatat tatgaaagaa
     1921 aagaaaaatt tggggataaa attaataaaa tccctatatg gtattaatgg cccattcgat
     1981 gaatacagag aacaaaaaat caattacctt ggaaatctat tatttgcaac cactagtctc
     2041 tatcacattt tatcctctat catcgcctgg ttcacggtaa gtaaatatcc cacagagact
     2101 gcatgtactc tcctagcttt aacattcttt ctatattttt gtatttttct aattgttatt
     2161 ccctcttatc tcaacagtca tgatttaata acgccactag aagtctcaaa aaatgactat
     2221 ccagtagcaa taaagaaaaa caaacaaaaa tttatcaaaa tcgggatatt ttatactgtc
     2281 atccagtggc ttatcttggg tttaacaact aacaataaca ctccgctgat tgtatcacta
     2341 ttttctttaa aagttatttt gtctagtatt atgtccggtg tagcttacaa tggagttatg
     2401 tatctaatat ttaaaaacaa tgttgatatt gaaaaagatg aataatgaca acatttcatc
     2461 tctaattgtt ctcactgtct atataaataa taaggctgga aaattttcca gccttattat
     2521 ttatggtaat atctttgcag cattaatcgc cgcttcgtag tcaacatctg aattaacatt
     2581 gtcaacgtat tcggtgtaag tcaccttatt atgctcatcc aaaataagaa ctgcacgcgt
     2641 taacaaatgc cactcattca tcaaaagagc gtactcttga ccaaacgaat gatcataaaa
     2701 atctgataat aaaatcacgt tatcaagacc tttagcgctg caccaacgct tctgagcaaa
     2761 tggaaggtcc atagaaacag taataaccac tgtgttatct agctctgata attcctcatt
     2821 aaaagtacgt gtttgttttg aacaaatacc tgtatcaata gaaggaacaa cgctaataac
     2881 tttctttttc ccttcaaaat cttttaaaga tttttgagat aagtctgtag ctattagtaa
     2941 aaaatctttt gcaatatccc caacttgtag ctgttttcct gtaaaagtaa caggatttcc
     3001 aagaaatgtc gtcataagtt tatctcctta tcaattacct ttattttact atgaagcttc
     3061 ttctttttcg aataattgaa ctgataatta gaggtactta cttccttcgc gtatgctcaa
     3121 agtcgacaac tcatcataat gattactgat aaagtccttt acccatactt tattatactt
     3181 tgaataatcc ctaagcgccc agccaatcgc cttattgata aaaaattctt gacttcctgt
     3241 atttcttaaa ataaaaagtg acaaaatatc agtatttgtt ttttctttaa atcctagttg
     3301 atgctcaata gctagtcgcc tcatccaaaa atcttgatca agtgaccatt ctgacagtaa
     3361 attcataacc ttgtcatctt ttaaggataa gttcccaaaa atcttagtca gaacatcaat
     3421 actatcccac cactgttgat gacgagcgta aaactctagt ctagagcaat cattataggt
     3481 gagaaattga ctcatagcta atagataatc aagtacaaaa tgatgatatt cacgatagtc
     3541 actttgccaa catttatcaa gcaattgcca atcaattttt gcttgtcttt tagcggcctt
     3601 tataagctct ttgtagagca tacgtcgctc aggtgattta ataccatagc acttaaaatg
     3661 atggcgtaaa taagtctcct gttgcttaga aacttctttg tcacttgcag ctttaaattt
     3721 cctctctaaa cttaggaata aatccattac gtaatcccct tgtatttttc aaaaagcatt
     3781 gtaattcctt catgtacaga ttctcttgat acacgtaaat tagcgtaagg agtcagttca
     3841 tcaaaatccc ctacttgaac actataatgc gcagcatcaa acatagaaat atcattttta
     3901 tcattgccaa aagctataaa acgattccca aataatttct tagtggcttt tcccttgtca
     3961 actgcaatgg gatttatgta caaatatttt tcaaattcgt ggaaaaagtg actactaagt
     4021 ccaaaacgat ttaatttcgc taacatttgg tctgcccttc tcagctgatc accaaaatac
     4081 aacaccattt ttatcggttt ctcaatttta gaaacttcta gaagctctcc ccttttttga
     4141 ggatcaacat aagcaataaa gggaatttta gaagcctgat aagtagcgta attaaagacc
     4201 tcatcaacaa aatatggaat ctgatagcga tgacaatatg aaataatagt actaaaaaag
     4261 tcactttgta aatagtaact atccactaag tgaccatttt catgtaaagt cgcaccattt
     4321 aaaccaataa tttttgataa agatagcttg tcgccaagga tacctatagt gtctctataa
     4381 gatcgcgccg tagcaaaagt gactctatga ccataatcaa tctgggctct ctctagtatt
     4441 ccttgtattt ctttagatag actcatacca tcaaaacaaa gagttccatc tatatcaaag
     4501 acaaaatcca ttctctcaaa gccccatcct taagttaaaa taggttttca tacgtgcata
     4561 aacatcttca tcaccaacaa aaaagatatg atctccttgc tctataactg agaaaggccc
     4621 tggagaaaca ataaatttcc cttcgtgttc aatagccaca atagtcgcac cagtttgatg
     4681 ccatacattt aattctccta ttgacttacc aaggtgttca gaatcttcac tgacaataat
     4741 ttcatatggt gctagtgggt attgtttact aaccgcacgt gtttgaagca aaaaatcatc
     4801 aactaaagta gctaactctt ccatttcttg ttgctgagcc ttaatattat cacggatttt
     4861 acctttcaaa atagcaactg aatgagaggt ttcatattgg tttaagaatg caattgcttt
     4921 ttctttagaa agaataatgg ctccactgcc atgctttaaa gttaaaatct gtaaatctgc
     4981 taaaatatta agacccttac gtgctgtttc tggtgatacg ttgaatgttg aagcaatagt
     5041 tgttctagat ttcaacttct ctccaacttc ataatctcca tttgcgattc tttgtgcaac
     5101 cgctactgct attttttgat atttagatgt tacaatttca gattgttcag aaaccatcca
     5161 accctcctta tcctaataaa aacatacata ttaaaccaca attagcaaaa tattgcaaat
     5221 atatcttttt tgttaatatc taatgtcttt agtaaatatc atataaaagg agctggtctc
     5281 ccagctccga gtgtcttatc ataaaaagta gtttactgaa ttaagattca ctagtacgag
     5341 cttctgagat tttttctttt gcagcctcag taccatttga tactgcttct tttgttttct
     5401 ctgctccaga agaaattgct gctttagctt tgccagcttg ttcagaagca aagtttcctg
     5461 ttgcttgtgc tgcatcagat acacgatctt gtactgtcac tgagtcagct tcatgttcag
     5521 ctttagtctt aacatctacg acatttgcgt tcaattcaac aactttaagg tgggtcatca
     5581 cttcaacgtt ttgactgaca atcgctttaa tgctttcaac gatagctggg atatccttgc
     5641 cgtattcaac aacgatgtct aaatcaactg caacttcttt agtaccaact tcaacattta
     5701 caccatcagt tactgagtca gaatttacaa cgctattttt tagattagag aagaaacctc
     5761 cactcacagc caagagtccg tctactgatt ctaaagcatg tccaacaatt ttagcaatta
     5821 ccttttcttc ataagttact gaagttttaa tatcttgttt ttgattttcc ataaataaat
     5881 gacctcaaac cttctagtcc tttttcttaa agctctctga agcaccctta atggtatctt
     5941 taatgtcagc aagtttttct tttgcatgac ctttcaattt atcgacttta ccttcagatt
     6001 caacttcttt atcgccagta agtttcccaa cagattcttt tactgaacca gaaaccttat
     6061 caacttttgc atcaaatttt tcttcagaca taatagcctc ctactaataa caatacttta
     6121 aactatactt aaatcaatta ttgtacacgt ggttcagact tcatgtcttc cactttatca
     6181 acaccgttac ctacggcact tttaacgttt gaaacttgat tcgaagcaaa ttcacctgtt
     6241 gcttgtgctg cacttgttac acgatcttga agagttactg aatcttcttc atgttgagca
     6301 cgagttttaa tatcaacaac attaacatta acttcaacca cttcaaggtc tgtcatacgt
     6361 ttcacttcag cttcaacaac ttttttgata tcggcaaaga tagttggaac atgtttttgg
     6421 tattcagcaa caatatccaa atcaacagca acttgctttt tacccacttc aacatttacg
     6481 ccatctgtaa ctgaatcaga atttacaacg ctatttttta aatttgagaa aaaaccacca
     6541 tttacagcca aaagaccatc tacatgctca atcgcaattc caacaatttt ttcaatcact
     6601 ttatcttcga aagttaattc tccacgaaca gctgtcgtgc cagaattgtt agttgtgttt
     6661 ttgatgtaag tttcagtcat aatagttcct ctttctttta tttaaataat gtagaataaa
     6721 tataaacgaa aaattttatt ttctcttatt aaagaattgg tcaagcatac ctgttctttg
     6781 cacataaagc ccagcataag cacctagcac aatgataaca agagcaagaa ttgttttaaa
     6841 gaatccaatg gttacaatca ttgcagctaa aacgagccca ataactgcac ctcctagtgg
     6901 atatctgtat ttcctcacga attctgacat attagactcc tattctacac gacttttatt
     6961 cgtcttagca tttccaacat tttcttcttc aacttgctta acaaatactc tagtgttcat
     7021 tttcttttcc aacccaaaga attcttttaa gccaagttca acatcacttt gaatatgttc
     7081 aagttgttcc ggtacatttg tacgagaagt taaaagacct ttaacatcaa ctttcacttt
     7141 tttcttatat aaatgagctg tcacactagg agatttcatc aaaccagttt ttaatacttc
     7201 tgttttaaca aatccctcta ttgcagattt ttttaacatc aatttaccag ttttatcagc
     7261 cagtttatac tctgtgtatc gtcttggata aaacagaact accaatatag ccagtaaaac
     7321 gattacaaac agcactaatg ctccccaaaa gaaataatga tacataccaa cgtcaatcgg
     7381 tgatggcaaa ttaaaatccc aatctaacca attaaaagat ggtagataaa tgtgttgttt
     7441 actgataaca gctacaaatc ctagtagggt aagggagatt agacctagca aagtatataa
     7501 actttttagt ccttttgaca taggtaactc cttttctagt tattcatttt acctagtaca
     7561 aatgaagtaa caatgacaac aataatcgca cctacgattg aaggaatcaa agccatacct
     7621 gctaatttag gcccccatgt tcctagtaaa ctttgaccaa caaatgaacc aactaaacca
     7681 gctaagatgt tagctatcca acccattgaa ccacctttgt tagtaacagc ccctgcaata
     7741 gcaccgataa tagcacctac gattaatgac caaattaaac tcatgataaa taccttctct
     7801 ttcttgacta atcattctac tagttatgta gttatgattt tttgcctaat acaaatgaag
     7861 taacaatgat aacgataatc gcacctacga ttgaaggaat caaagccata tctgctaact
     7921 taggccccca tgttcctagt aaactttgac caacaaatga accaactaaa ccagctaaga
     7981 tgttagctat ccaacccatt gaaccacctt tgttagtgat tgctcctgca atagcaccaa
     8041 taatagcacc aacgattaat gaccaaatta accccatgat aaataccttt cttcctaagt
     8101 acatctcagt gtacaaactt attttgttct taactaaata acaagagttt ctaaagtgac
     8161 cactcggcaa ttaattaagt tgttaactat actaatatta tacaacttta taaagtgact
     8221 gccaagtaaa aagcttggta aatgctttta tttttaagtg accacttgat atttaattga
     8281 tattgttatt atactc
//
LOCUS       DATROD010000077         8220 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.077, whole genome shotgun sequence.
ACCESSION   DATROD010000077 DATROD010000000
VERSION     DATROD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8220
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.077"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..1165
                     /locus_tag="VBC74_001526"
     CDS             <1..1165
                     /locus_tag="VBC74_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HEO6942464.1"
                     /translation="FISCVLALCYRLFRPSFIEKNDLLKEISDITIFWLIIRLIGLAL
                     GLMTVLHIGPEMVCGKETGGLILFDLIGGLFTIFLAAGFILPFLTEFGLLEFVGVFLT
                     PIMRPFFQLPGRSAVNCVASFVGDGTIGIALTDKQYVEGYYTSREAATISTTFSAVSI
                     TFCLVVLQNVGLTDYFGHFYLTVIISSVVCAFIIPYIPPLSRQSNSYYTKPKKETSEV
                     IPDGYNKIEWGLKLAIDKSKANGNMKTFVNNASKTVLSLWFGVMPTIMTVGTIALIIS
                     VSTPIFKILGTPFLPFLELLGIPEADIASQTMIVGFSDMVVPSIMAAEIHSEMTRFIV
                     ATVSIVQLIYMSETGAVILGSKIPINILELFIIFIERTIISLPIIVLMAHLFF"
     gene            1349..1483
                     /gene="rpmH"
                     /locus_tag="VBC74_001527"
     CDS             1349..1483
                     /gene="rpmH"
                     /locus_tag="VBC74_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEO6942465.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            1839..2513
                     /locus_tag="VBC74_001528"
     CDS             1839..2513
                     /locus_tag="VBC74_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6942466.1"
                     /translation="MRLLVVEDEKSIAEAIQALLADKGYSVDLAFDGDDGLEYILTGL
                     YDLVLLDIMLPKRSGLSVLKRVREAGLETPIIFLTAKSQTYDKVNGLDLGADDYITKP
                     FEADELLARIRLRTRQSSLIRANQLRLGNIRLNTDSHELESKESSVKLSNKEFLLMEV
                     FMRNAKQIIPKNQLISKVWGPSDNSEYNQLEVFISFLRKKLRFLKADIEIITTKGFGY
                     SLEERT"
     gene            2513..3700
                     /locus_tag="VBC74_001529"
     CDS             2513..3700
                     /locus_tag="VBC74_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6942467.1"
                     /translation="MFSDLRKKFVFLTMSILIVVVLFLFAVSNRYNQYWDEYDAYRIV
                     KLVAKNDYLGIPGDEPIALVTIDNQKMVKIQSNNTDLTNDVIEKSSLKLLEQGKKSRK
                     WKSFIYSIKEYKDKTYTIAIMDLASYEVPYARRFLILVFTIFGFCLLAAVSLYLSRFI
                     VGPVETEMTREKQFVSDASHELKTPIAAIRANVQVLEQQIPGNRYLDHVVSETKRMEF
                     LIEDLLNLSRLDEKRSKVNFKKLNLSVLCQEVLLTYESLAYEEEKCLNDTIEDDVWIV
                     GEESQIKQILIILLDNAIRHSLSKSAIQFSLKQARRKAILTISNPSAIYSKEVMDNLF
                     ERFYQAKDDHADSLSFGLGLSIAKAIVERHKGRIRAYQEKDQLRLEVQLPIDGFWTNT
                     MIN"
     gene            3711..3833
                     /locus_tag="VBC74_001530"
     CDS             3711..3833
                     /locus_tag="VBC74_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEO6942468.1"
                     /translation="MKPYLSFIGRTLLYFGILLLLIYFFAYLGRGQGSFIYNEF"
     gene            3846..5381
                     /gene="dltA"
                     /locus_tag="VBC74_001531"
     CDS             3846..5381
                     /gene="dltA"
                     /locus_tag="VBC74_001531"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEO6942469.1"
                     /translation="MIHDMIKTIEHFAETQADFPVYDILGEVHTYGQLKVDSDSLAAH
                     IDSLGLVEKSPVLVFGGQEYEMLATFVALTKSGHAYIPVDQHSALDRIQAIMTVAQPS
                     LIISIGEFPLEVDNVPILDVSQVSAIFEEKTPYEVTHSVKGDDNYYIIFTSGTTGLPK
                     GVQISHDNLLSFTNWMISDDEFSVPERPQMLAQPPYSFDLSVMYWAPTLAMGGTLFAL
                     PKTVVNDFKKLFATINELPIQVWTSTPSFADMALLSNDFNSETLPQLTHFYFDGEELT
                     VKTAQKLRQRFPKARIVNAYGPTEATVALSAVAITDEMLETCKRLPIGYTKDDSPTYV
                     IDEEGHKLPNGEQGEIIIAGPAVSKGYLNNPEKTAEAFFQFEGLPAYHTGDLGSMTDE
                     GLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSQYVKSAVAVPRYNKDHKVQNLLAYIV
                     LKEGVRDDFERDLDLTKAIKEDLKDIMMDYMMPSKFIYREDLPLTPNGKIDIKGLMSE
                     VNK"
     gene            5378..6643
                     /gene="dltB"
                     /locus_tag="VBC74_001532"
     CDS             5378..6643
                     /gene="dltB"
                     /locus_tag="VBC74_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEO6942470.1"
                     /translation="MIQAFLEKLPHLDVYGNPQYFFYLILAVLPIYIGLFFKKRFALY
                     EIIFSLSFIVMMLTGSTFNQLKSLLAYVVGQSLLVFIYKAYRKRFNHTLVFYVTVCLS
                     IFPLFLVKLIPAISEDGHQSLFGFLGISYLTFRAVAMIIEMRDGVLKEFTLWEFLRFL
                     LFFPTFSSGPIDRFKRFNEDYINIPDRNELLDMLGQAIHYLMLGFLYKFILAYIFGSL
                     IMPPLKELALEQGGVFNWPTLGVMYAFGFDLFFDFAGYTMFALAISNLMGIKSPINFD
                     KPFKSRDLKEFWNRWHMSLSFWFRDFVFMRLVKLLVKNKVFKNRNVTSSVAYIINMLL
                     MGFWHGLTWYYIAYGLFHGIGLVINDAWVRKKKNINKERRLAKKPLLPENKWTYALGV
                     FITFNVVMFSFLIFSGFLDLLWFPQPHNK"
     gene            6658..6897
                     /gene="dltC"
                     /locus_tag="VBC74_001533"
     CDS             6658..6897
                     /gene="dltC"
                     /locus_tag="VBC74_001533"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEO6942471.1"
                     /translation="MDIKSEVLAIIDDLFMEDVSSMMDEDLFDAGVLDSMGTVELIVE
                     LESHFNIDIPIAEFGRNDWNTANKIVAGVTELCNA"
     gene            6890..8152
                     /gene="dltD"
                     /locus_tag="VBC74_001534"
     CDS             6890..8152
                     /gene="dltD"
                     /locus_tag="VBC74_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEO6942472.1"
                     /translation="MLKRLGKVFGPLVCALLLLVGLYFVFPVSQPHHLGKEKNSAVAL
                     TKAGFKSRVQKVRAFSDPKANFVPFFGSSEWLRFDAMHPSVLAEAYNRSYIPYLLGQK
                     GAASLTQYYGIQQIKGQIKNKKAIYVISPQWFVRKGANKGAFQNYFSNDQTIRFLQNQ
                     TGTTYDRYAARRLLKLYPEASMSDLIEKVADGQKLSNKDKQRLKFNDWVFEKTDAIFS
                     YLPLGKTYNQAIMPHVGKLPKAFSYNHLSRIASQDAKVATRSNQFGIDDRFYQTRIKK
                     HLKKLKGSQRHFNYTKSPEFNDLQLVLNEFSKQNTDVLFVIPPVNKKWTDYTGLDQKM
                     YQKSVEKIKHQLQSQGFNHIADLSRDGGKPYFMQDTIHLGWNGWLELDKHINPFLTEE
                     NSKPNYHINNKFLKKSWAKYTERPSDYK"
BASE COUNT         2592 a         1292 c         1573 g         2763 t
ORIGIN      
        1 ttttatttca tgtgtgttag cactttgtta tcgtctgttt agacctagct ttattgaaaa
       61 aaatgacttg ctaaaagaaa tttctgacat taccattttc tggctgatta ttcgtttgat
      121 cggtttggca cttggtctga tgacggtttt acatattggt cctgaaatgg tatgtgggaa
      181 agagacaggc ggccttatcc tttttgattt gattggtggt ttgtttacca ttttccttgc
      241 tgcgggtttt attcttccat ttttgacaga atttggtcta ctggagtttg ttggtgtctt
      301 cctaactccc attatgcgtc cattctttca gctacctggt cggtcagcag ttaattgtgt
      361 agcctcattt gtaggtgatg gtactattgg aattgctttg accgataagc agtatgttga
      421 gggttattat accagtagag aagctgcaac gatttccact actttttcag cagtatcaat
      481 cactttttgt cttgttgttt tgcagaatgt tggcttaact gattattttg gccattttta
      541 tctaacagtc atcattagca gtgttgtctg tgcattcatt atcccttata tccctccctt
      601 atctcgtcaa tcaaatagtt attacactaa accaaagaaa gaaacatctg aggtaattcc
      661 ggatggttat aacaagatag agtggggact taagcttgcc atcgataaat caaaagcaaa
      721 tggaaacatg aagacttttg tcaataatgc ctctaagaca gtacttagtt tatggtttgg
      781 cgttatgcca acaattatga ctgtcggaac aatagctttg attatttcag tatcaacacc
      841 tatttttaaa attttaggta caccattcct tccgttcctt gaacttttag ggattccaga
      901 agcagatatt gcgagtcaaa ccatgattgt tggtttttca gatatggttg ttccttcgat
      961 aatggcggct gaaattcaca gtgagatgac aaggtttatt gtcgcaacag tttctattgt
     1021 gcaattaatt tatatgtctg agactggtgc ggtgatatta ggttccaaga tccctatcaa
     1081 cattctagaa ttatttatca tttttataga gagaacaatt atttcattac ctattattgt
     1141 tttgatggca catcttttct tttagttgga gaaaatagag aagcatttct tgctaacaaa
     1201 tagaactatt tactgaaaaa agacaaatga acttgaaagt atttcttgac aagaggccta
     1261 aaattgttat aaaatagtat ggtatgttta gtaactgatc aaaatagccg gctaaacgaa
     1321 tacgaaaatc tatcaggagg tattcaacgt gaaacgtact tatcaaccaa gtaaaatccg
     1381 tcgccaacgt aaacacggat tccgtcaccg tatgtcaact aaaaacggac gtcgcgtgct
     1441 tgctagccgc cgtcgtaaag gacgtaaagt gttatcagct taatatagat agcaaaataa
     1501 tatcaagcca acaggtactc gagtctggtg gcttttttgg taccttcata acgataaagg
     1561 tttttgtgag agtagtttga gtatattata gataaagtta atgaaaagtg agtttataat
     1621 gcggaaattc gtagacaaat agcccctatt tttgttagaa tatgaggaga tttcttattg
     1681 agaaatatct ttatagcgag tgatatttta ttttttagac gcttgtatta ttgaagtagt
     1741 ttagtgactt aggaaatact tttattaata tcgttttttc aaggttattt caattttgct
     1801 agtttatact ataatatata aggtaaggtg actagaaaat gagacttctt gtagttgagg
     1861 atgaaaaaag tatagcagag gctattcagg ctttattggc agataaaggt tattctgttg
     1921 atttagcatt tgatggagat gatgggttag aatacatttt gacaggtctc tatgatttag
     1981 tcctgttgga tattatgtta cccaaacgct caggattatc tgttttaaaa agagtgaggg
     2041 aagctggttt agaaacccca ataatctttt taactgcaaa atcacagact tatgataaag
     2101 ttaatggttt agatttaggg gcagatgatt atatcacaaa accctttgaa gcagatgaat
     2161 tgctagctag aattaggctg agaaccagac aatcttctct aatcagagca aatcaattgc
     2221 ggcttggaaa tataaggctt aacacagatt cacatgagct agaatctaaa gaaagtagtg
     2281 taaaattatc caataaagaa tttctcttga tggaagtttt tatgcgaaat gcaaagcaga
     2341 ttattcctaa aaatcagttg atttctaagg tttggggacc ttcagacaat tcagaatata
     2401 accagttaga agtatttatt tcttttttgc gaaaaaaatt gcgcttttta aaagcagaca
     2461 ttgaaataat aaccactaag ggctttggat attccttgga ggagaggaca taatgtttag
     2521 tgatttacgc aaaaaatttg tctttttgac catgtcaatc cttattgtcg ttgttctctt
     2581 tctatttgca gttagtaatc gctacaatca atattgggat gaatatgatg catataggat
     2641 tgttaaatta gttgccaaaa atgattattt aggaatacca ggcgatgaac cgattgcttt
     2701 ggtgactata gataaccaaa agatggttaa gattcaaagc aataatactg atttgacaaa
     2761 tgatgttatc gaaaaatcta gtttgaagct ccttgagcaa ggaaagaagt cacgaaaatg
     2821 gaagtctttt atatattcca taaaagaata taaagataaa acctacacca tagctattat
     2881 ggatttggct agttatgaag tcccttatgc aaggcgtttt ctaattcttg tttttactat
     2941 ttttggattt tgtttattag cagctgtaag tctttatctt tctcgattta ttgtaggacc
     3001 tgtagagaca gaaatgactc gcgaaaaaca gttcgtttca gatgcgagtc atgagctgaa
     3061 gacccctatt gcagctatac gagccaacgt tcaggttctg gaacagcaaa ttccagggaa
     3121 tcgttatctg gatcatgttg tttcggagac taagcgaatg gagtttttaa ttgaagattt
     3181 gcttaatttg tctagacttg atgaaaaacg ctccaaggta aatttcaaaa agttaaactt
     3241 atctgtctta tgtcaagaag tacttctaac atatgaaagt ttggcatatg aagaagaaaa
     3301 gtgtttaaat gatacgattg aagatgatgt ctggattgtt ggagaagaga gtcagattaa
     3361 gcaaatttta ataattcttt tggataatgc tattaggcat tctttgtcaa aatcagcaat
     3421 ccagtttagt cttaaacaag ctcgaagaaa agctattctg acaatatcaa atccaagtgc
     3481 tatctattcc aaagaggtta tggataatct ctttgagcgt ttttatcaag ctaaagatga
     3541 tcatgcagat tctctcagtt ttggtctagg cttatctatt gctaaagcaa tcgtggaacg
     3601 ccataagggg agaattagag catatcaaga aaaagaccaa ttacgtcttg aagtccagtt
     3661 accaattgat ggattttgga ccaataccat gattaattaa aggaagaatg atgaaaccat
     3721 atttatcttt tattggtaga acgttattat acttcggtat tttattgtta ctaatttact
     3781 tttttgcata ccttggtcgc ggacaaggca gttttattta taatgaattc taggagatgg
     3841 tacaaatgat acatgatatg attaaaacaa ttgagcattt tgctgagaca caagctgatt
     3901 ttccagtgta tgatatttta ggggaagtcc atacttatgg acaacttaaa gtagactctg
     3961 actctctagc tgctcatatt gatagcctag gccttgttga aaaatcacct gtcttagtat
     4021 tcggtggtca agaatatgaa atgttggcga catttgttgc tttaacaaag tcagggcatg
     4081 cttatatacc ggttgaccaa cactctgctt tggatagaat acaggctatt atgacagttg
     4141 ctcaaccaag ccttatcatt tcaattggtg aatttcctct tgaagttgat aatgtcccaa
     4201 tcctagacgt ttctcaagtt tcagctattt ttgaagaaaa gactccttat gaggtaacac
     4261 attctgttaa aggtgatgat aattactata ttattttcac ttcagggact actggtttac
     4321 caaaaggtgt gcaaatttca catgacaatt tattgagctt tacaaattgg atgatttctg
     4381 atgatgagtt ttcagttcct gaaagaccgc aaatgttggc tcaaccgcca tattcatttg
     4441 acttatcagt tatgtattgg gcaccaacac tagctatggg aggcaccctg tttgccctac
     4501 caaaaacagt agttaatgat ttcaaaaaac tattcgctac cattaatgaa ttgccaatac
     4561 aggtttggac ttcgacacca tcatttgctg atatggcgct actatctaac gatttcaatt
     4621 cagagacctt gccacagtta acacattttt attttgatgg ggaagagtta actgtcaaga
     4681 ctgcacaaaa acttcgtcaa cgttttccaa aagctcgtat cgttaatgca tatgggccaa
     4741 cagaagcaac agttgctcta tccgcagtag caattactga tgaaatgtta gaaacatgca
     4801 aacgccttcc aattggttac actaaagatg actcaccaac gtatgtgatt gatgaagaag
     4861 gtcataaatt accaaacgga gagcaaggtg aaatcattat tgctggacca gcagtatcaa
     4921 aaggctatct taacaatcca gaaaagacag ctgaggcatt tttccaattc gaaggtctac
     4981 ctgcttacca taccggtgac ttaggaagta tgaccgatga aggtcttctg ctttacggtg
     5041 ggcgtatgga tttccaaatt aaatttaacg gctatcgtat tgaattagaa gatgtttctc
     5101 aaaacttaaa caaatcgcag tatgtaaaat cagcagtagc agtgccacgt tataacaagg
     5161 atcataaagt tcaaaactta ttagcctata ttgtcttaaa agaaggtgta agagatgatt
     5221 ttgaacgtga tttggatttg acaaaagcaa ttaaggaaga cttaaaggac attatgatgg
     5281 attacatgat gccatctaaa tttatctatc gagaggattt acctttgaca ccaaatggga
     5341 aaattgatat caaaggtctt atgagcgagg taaacaagtg atacaggcat ttttagaaaa
     5401 acttcctcac ttggatgttt atgggaatcc acaatatttc ttctacctta tcttagctgt
     5461 cctaccaatt tacatcggct tattctttaa gaagcgtttt gccttatatg agattatttt
     5521 tagtctaagt tttattgtaa tgatgttgac tggtagtact tttaatcaat tgaagtcact
     5581 attggcatac gttgtcggac agtctctgct agtttttatc tataaagctt accggaaacg
     5641 atttaatcat actttggtct tttatgtaac ggtttgttta tctatttttc cgctattttt
     5701 ggtaaaatta attccagcta tatctgagga tgggcatcag tcactttttg ggtttctagg
     5761 aatttcttac cttactttta gagctgttgc tatgattatt gaaatgagag acggtgtctt
     5821 gaaagaattt actttatggg aattcttaag atttttactc ttctttccaa ctttctcaag
     5881 tggaccaatt gatcgtttta aacgattcaa tgaggattac attaatatcc cagatcgaaa
     5941 cgaactccta gatatgttag gtcaagcgat tcattatttg atgttaggtt ttctctataa
     6001 gtttatttta gcctatattt ttggaagtct gattatgcct cctctaaaag aattagcgct
     6061 agaacagggt ggtgtgttta attggccaac acttggggtt atgtatgcct ttggttttga
     6121 tttgttcttt gattttgcag gttacacaat gtttgcgttg gctatttcta acctaatggg
     6181 gattaagtct ccgattaact ttgacaaacc tttcaaatca cgcgacctaa aagaattttg
     6241 gaatagatgg catatgagcc tttctttctg gtttagagac tttgttttca tgaggcttgt
     6301 taagctttta gttaaaaata aagtttttaa aaaccgtaat gttacttcaa gtgtagctta
     6361 tattatcaat atgcttctta tgggattctg gcatgggtta acttggtact atatagccta
     6421 tggtctcttt catgggattg gcctagttat taatgacgct tgggtacgta agaagaaaaa
     6481 tattaataaa gaaagaagat tggctaaaaa accactttta ccagaaaaca aatggactta
     6541 tgctttgggt gtcttcatca cctttaatgt agttatgttt tctttcttga ttttttcagg
     6601 atttttagat cttttgtggt tcccacaacc gcataacaaa taataaggag tattataatg
     6661 gatattaaat cagaagtatt agcaattatt gatgacctat ttatggagga tgtttcttct
     6721 atgatggatg aagacttatt tgacgcagga gttctagaca gcatgggtac tgttgaacta
     6781 attgttgagt tggaaagcca ctttaatatt gatattccga ttgcggaatt tggtcgtaat
     6841 gactggaaca cagctaataa aattgttgct ggagtaacgg agctctgcaa tgcttaaacg
     6901 tctaggtaaa gtatttggac ctctagtttg tgctttacta ttgttggtag gattatattt
     6961 tgtttttcct gtttctcagc ctcatcattt aggtaaggaa aaaaacagtg cagtagcgtt
     7021 gacgaaggca ggttttaaaa gcagagttca aaaagttaga gctttcagtg atcctaaagc
     7081 caattttgtc cctttctttg gttcaagtga gtggctaaga tttgacgcaa tgcatccatc
     7141 agttttagca gaggcttaca ataggtctta tatcccatat cttttaggtc aaaaaggggc
     7201 ggcttctctg acacaatact atggcattca acagattaaa ggacaaatca aaaataaaaa
     7261 agctatctat gttatttctc cgcaatggtt tgttcgcaag ggagccaaca aaggtgcttt
     7321 tcaaaactat ttcagcaacg atcaaaccat tcgatttttg caaaatcaaa cagggacaac
     7381 ctacgatagg tatgctgctc gtcgattgtt aaaattatat cctgaagctt ctatgtcaga
     7441 tttgatagaa aaagttgcag atggccaaaa actatcaaat aaagacaaac aaagactaaa
     7501 gtttaatgat tgggtatttg agaagacaga tgctattttt agctatctac cactaggaaa
     7561 aacttataat caggcaataa tgcctcatgt tggtaaatta ccgaaagcat tctcatataa
     7621 tcatttatcg cgtattgcat cacaagatgc taaagtagca acgagatcaa atcaatttgg
     7681 tattgatgat cgcttttacc aaacaagaat taaaaagcac ttaaaaaaat tgaaagggtc
     7741 acaacgacat ttcaattata ctaagtcacc agaatttaat gatttacagt tggttcttaa
     7801 tgaattctca aaacaaaata cagatgtcct ttttgtcata ccaccagtaa ataaaaagtg
     7861 gacagactac acagggcttg atcaaaaaat gtatcaaaaa tctgtagaaa aaataaaaca
     7921 ccaacttcaa agtcaaggtt tcaatcatat cgctgacctt tctcgagatg gaggtaagcc
     7981 atactttatg caagatacaa tccatttagg ttggaatggt tggttagagc tagataagca
     8041 tatcaatcca tttttaacag aggaaaacag caagccgaat tatcacatta ataataaatt
     8101 tttgaagaaa tcttgggcaa aatatacaga acgtccaagt gattacaagt aaatagtaga
     8161 gtcagatgat ttatagcatt agagctatta atcatctttt ttaatatctt tgtatttggt
//
LOCUS       DATROD010000078         8126 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.078, whole genome shotgun sequence.
ACCESSION   DATROD010000078 DATROD010000000
VERSION     DATROD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.078"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..1059
                     /gene="xerS"
                     /locus_tag="VBC74_001535"
     CDS             <1..1059
                     /gene="xerS"
                     /locus_tag="VBC74_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEO6942473.1"
                     /translation="LLLEKIDELKEIMPWYVLEYYQSKLSVPYSFTTLYEYLKEYRRF
                     LEWLLDSGVANCHHIAEIELSVLENLTKKDMEAFILYLRERPLLNANTRQNGVSQTTI
                     NRTLSALSSLFKYLTEEVENADGEPYFYRNVMKKVSTKKKKETLASRAENIKQKLFLG
                     NETIEFLEYIDCEYQNKLSKRALAFFNKNKERDLAIIALLLASGVRLSEAVNLDLKDI
                     NLNVMVIDVTRKGGKRDSVNVASFAKPYLANYLDIRKNRYKAENQDIALFLSEYRGVP
                     NRIDSSSVEKMVAKYSQDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHASTQVT
                     DLYTHIVNDEQKNALDKL"
     gene            complement(1102..2040)
                     /locus_tag="VBC74_001536"
     CDS             complement(1102..2040)
                     /locus_tag="VBC74_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6942474.1"
                     /translation="MIRQFLREHLIWYILYIMMFVLFFISFYLYHLPMPYLFNSLGLN
                     VIVLLGISIWQYSRYRKKMLHLKYFNSSQDPSFELQPSDYAYFNIITQLEAREAQKVS
                     ETIEQTNHVALMIKMWSHQMKVPLAAISLMAQTNHLDPKEVEQQLLKLQHYLETLLAF
                     LKFRQYRDDFRFEAVSLREVVVEIIKSYKVICLSKSLSIIIEGDNIWKTDKKWLTFAL
                     SQVLDNAIKYSNPESKIIISIGEESIRIQDYGIGILEEDIPRLFEDGFTGYNGHEHQK
                     ATGMGLYMTKEVLSSLNLSISVDSKINYGTAVSIHK"
     gene            complement(2037..2705)
                     /locus_tag="VBC74_001537"
     CDS             complement(2037..2705)
                     /locus_tag="VBC74_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6942475.1"
                     /translation="MSQEQGKIYIVEDDMTIVSLLKDHLSASYHVSSVSNFRDVKQEI
                     IAFQPDLILMDITLPYFNGFYWTAELRKFLTIPIIFISSSNDEMDMVMALNMGGDDFI
                     SKPFSLAVLDAKLTAILRRSQQFIQQELTFGGFTLTREGLLSSQDKEVILSPTENKIL
                     SILLMHPKQVVSKESLLEKLWENDSFIDQNTLNVNMTRLRKKIVPIGFDYIHTVRGVG
                     YLLQ"
     gene            complement(2815..4770)
                     /locus_tag="VBC74_001538"
     CDS             complement(2815..4770)
                     /locus_tag="VBC74_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6942476.1"
                     /translation="MFYLKIAWHNLKHSIDQYIPFLLASLLLYSLTCSTLLILMSAVG
                     RDMGTAATVLFLGVIVLSIFAVVMEHYSYNILMKQRSSEFGLYNILGMNKRQVARVAS
                     LELFIIYIFLISIGSLFSAFFAKFIYLIFVNIINYHALNLSLSLWPFIICIVIFTGIF
                     LTLEVPVIRHVHLSSPLSLFRKKQQGEKEPKGNLILAILALVAIAIAYTMALTSGKAP
                     ALAVIYRFFFAVLLVIAGTYLFYISFMTWYLKRLRQNKHYYYKSEHFVSTSQMIFRMK
                     QNAVGLASITLLAVMALVTIATTVSLYSNTQNVVTGLFPKSVSLSIDNSKGDAKNIFE
                     EKILKKLGKSSKEAITYNQTMISMPVSQSSELNITSKNVKHVDITKTGFMYLITQNDF
                     RRLGHQLPKLKDNQVAYFVQKGDSRLKKINLLGNKFDVVKNLKEAYVPETTNTYNPGL
                     IIFANNKQIDNIRKAYLPYTKNINTFPKTFKAYLDLNSQEINSISKNDIIEVDGKYVG
                     NISTKQSFLKEGYQMFGGLLFTGFLLGISFLLGIALIVYYKQYSEGHEDKRSYRILQE
                     VGMSKKLVKRTINSQIMIFFFQPLVVAVIHFGVAIPMLKQMLLVFGVLNSTIVYVVSG
                     LTVLAISIIYFIIYRITSRTYYHIIER"
     gene            complement(4772..5524)
                     /locus_tag="VBC74_001539"
     CDS             complement(4772..5524)
                     /locus_tag="VBC74_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6942477.1"
                     /translation="MLLEINHLEKVFRTRFSKEETRALQDVDFKVEQGEFIAIMGESG
                     SGKTTLLNILATLEKPTNGQVILNGEDITKIKEAKLASFRLKNLGFVFQDFNLLDTLS
                     VRDNIYLPLVLDRKRYKEMDHRLSELSSHLRIDDLLDKRPFELSGGQKQRVAIARSLI
                     TNPQILLADEPTAALDYRNSEDLLNLFETINLDGQTILMVTHSANAASHAKRVLFIKD
                     GRIFHQLYRGNKNNSEFNKDISLTMSAILGGE"
     gene            complement(5550..6512)
                     /locus_tag="VBC74_001540"
     CDS             complement(5550..6512)
                     /locus_tag="VBC74_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEO6942478.1"
                     /translation="MRRKIVLLFVVPMLIVLGILGVVVHYYGSALNIYLLPPSSERYG
                     RVILDRVEQRGLYSQGRQWQIIRQRSEKKLKTSKSYQESRNIVQEAVRYGGGKHSQIL
                     SKETVRRDTLDSRYPEYRRLNEDILLITIPSISKLDKRSISHYSGKLQNILMEKSYKG
                     LILDLSNNTGGNMIPMIGGLASILPNDTLFHYTDKYGNKKTITMKNIPLEALKISRKT
                     INTKHVPIAIITNHKTASSAEMTFLSFKGLPNVKSFGQATAGYTTVNETFMLYDGARL
                     ALTTGIVSDRQGYKYENTPILPDQVTSLPLQESQSWLKSRINQN"
     gene            6678..7166
                     /locus_tag="VBC74_001541"
     CDS             6678..7166
                     /locus_tag="VBC74_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEO6942479.1"
                     /translation="MAKTSKKLTKSLGPIGKLISIIPDTTELIGKAIDNSRPIIEKEL
                     DRRHEKKQTYTQLDNVVHLDINDAQVYLEQRHFNVSRIVAEPNSKWATLHPNQVVNMS
                     PKAGRVRIGSLVKLYYITEDILEESKTLLLSKSEKSKARQQRLTEVLSTPSQLLKKNR
                     NK"
     gene            complement(7198..8046)
                     /locus_tag="VBC74_001542"
     CDS             complement(7198..8046)
                     /locus_tag="VBC74_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEO6942480.1"
                     /translation="MKIKKLLGLTTTVVISALILGACGQSKNEDAKVVRVGTMVKSKT
                     EKARWDKIEELVKKKGVKLKFTEFTDYTQPNKALESDEIDINAFQHYNYLNNWNKANK
                     TNLVSVAETYFTSFRLYSGTKNGKGKYQTVSEIPNKATITIPNDAVNESRSLYLLQSA
                     GLLKLKVSGDTLATMSDVVSNPKSLDLKEVDAAQTARSLDSTDAAVINNDFVTEAGIN
                     PKSAIFIEPKSKNAKQWYNLLVAQKGWQDKSKAKAIKEVVKAYHTDAVKKVIEKTSQG
                     LDQPVW"
BASE COUNT         2712 a         1414 c         1249 g         2751 t
ORIGIN      
        1 ttactactcg aaaaaatcga tgaactaaaa gaaattatgc cttggtatgt tctagaatat
       61 tatcaatcaa agctttctgt tccttatagt ttcacaacac tctacgaata tttaaaagaa
      121 tatagacgtt ttcttgaatg gttattggat tctggtgttg ctaattgtca tcacattgca
      181 gaaattgagc tttcagtttt agaaaacctt actaaaaaag atatggaagc ttttatacta
      241 tatttacgag aacgcccttt attaaatgct aacacaaggc aaaatggggt gtctcaaacc
      301 accattaaca gaactctatc tgccttatct agtcttttca aataccttac tgaagaggtt
      361 gagaatgctg acggggagcc ttacttttat cgcaatgtca tgaaaaaagt ctcaaccaaa
      421 aagaaaaagg aaactttagc ttctcgggca gaaaatatta aacaaaagtt atttttgggc
      481 aatgaaacga tagaattctt agagtatata gattgtgaat atcaaaacaa actttccaaa
      541 cgtgctttag cttttttcaa caaaaataaa gagagagatt tagctattat tgctttgcta
      601 ctagcttcag gagtgcgtct atctgaggca gtaaacttag atttaaagga tattaacctt
      661 aatgtaatgg ttattgatgt cacacgaaaa ggggggaaaa gggactctgt caatgtcgct
      721 agctttgcga agccctacct tgctaattat ttagacatcc gtaaaaatag atacaaagca
      781 gaaaatcagg acattgctct atttttatca gaataccgtg gggtgccaaa tcgtattgat
      841 tcttcaagtg tagagaaaat ggttgctaag tattcccagg attttaaggt tcgtgtaaca
      901 cctcataaac taaggcatac tcttgctact aggctatatg atgctacaaa atctcaagta
      961 ttagttagcc accaactagg tcatgcttcc actcaagtta ctgacctata tacccatatt
     1021 gttaacgatg aacaaaagaa cgctttagat aaactatagt aacataaata aagttatgta
     1081 aatattttat tttacataac tttatttatg tatagaaaca gcagtcccat aattaatttt
     1141 gctatccacc gaaatggaca aattcagact agataagact tcttttgtca tatataaccc
     1201 catgcctgtt gccttttggt gctcatgacc gttgtaaccc gtaaagccat cttcaaaaag
     1261 tctagggata tcctcttcga gtatgccgat accgtagtct tgtattctaa tactctcttc
     1321 tcctatgctt attattatct ttgactcagg attagaatat tttatggcat tatctagcac
     1381 ctgtgaaaga gcaaaagtta accacttttt gtctgttttc cagatattat cgccttcaat
     1441 tatgatagat aagcttttgg atagacaaat aaccttatac gattttataa tttctactac
     1501 tacttctcta aggctaacag cttcaaaacg aaaatcgtca cgatattgtc taaatttcaa
     1561 aaatgctaac aacgtttcaa gataatgttg caatttcaat aattgttgtt caacttcctt
     1621 aggatcgaga tgatttgtct gggccattaa tgaaatagct gccaatggaa ctttcatttg
     1681 gtgcgaccac atctttatca taagtgcaac atgattggtt tgttcaattg tttcagaaac
     1741 tttttgcgct tctctagctt ctaattgtgt aataatatta aaataagcgt aatcactcgg
     1801 ttgaagttcg aaagaggggt cctgactact attaaaatat ttgagatgta acattttttt
     1861 cctgtaacga ctgtattgcc aaatactaat tcctagtaaa acaataacat ttaaacctaa
     1921 ggaattaaac aaatagggca ttggtaaatg atatagatag aaactaataa aaaataggac
     1981 aaacatcatg atatataaaa tataccaaat caagtgttct cttaaaaact ggcgtatcat
     2041 tgtagtaaat acccaactcc tctcactgta tgaatgtaat caaaacctat tgggacaatt
     2101 tttttacgta agcgtgtcat attaacatta agtgtatttt gatcaataaa actatcattt
     2161 tcccaaagtt tctctaatag agactctttt gagactactt gtttaggatg catgagcaag
     2221 atagatagga ttttattttc tgttggcgat aaaataacct ctttatcttg gctagacaat
     2281 aacccttctc ttgtcaacgt aaatccccca aaagttaatt cctgttggat aaattgttga
     2341 cttctcctta aaatagcagt tagcttagca tctaatacag ctagagagaa tggttttgaa
     2401 ataaagtcat caccccccat atttaatgcc ataaccatat ccatttcatc attactagat
     2461 gaaatgaaaa taataggaat tgttaaaaac ttacgcaatt ctgcagtcca gtaaaaacca
     2521 ttaaaatagg gtaacgtaat atccattagt atcaaatcgg gttgaaatgc gataatttct
     2581 tgtttcacat cacgaaaatt gctgacacta gagacatgat agctagctga taaatgatct
     2641 tttaaaagtg acacaatcgt catatcatct tctacaatat aaatttttcc ttgctcttgt
     2701 gacatatccc ctcctaaaat agtacgaaat ctctttcttt catttagtat aacaaatttt
     2761 ttagtctatc ggtttctatt aatgcaaaag gattggtaaa ataaccaatc ctttttagcg
     2821 ttcaataata tgataataag ttcgactagt tatacggtaa atgataaagt aaattataga
     2881 aatcgcaagt actgtcaaac cacttactac atatacgatt gttgagttaa gcaccccaaa
     2941 taccaaaagc atctgtttta gcattggaat agcaactcca aagtgaataa ctgctaccac
     3001 taaaggttgg aagaagaaaa tcataatttg agaattaata gtccgtttaa ctaatttttt
     3061 gctcatacct acttcttgta agatacgata agatctctta tcttcatgac cttctgagta
     3121 ttgcttatag taaacaatta gagctatacc tagcaaaaag cttataccta acagaaatcc
     3181 agtaaataat aacccaccaa acatttgata accttctttt agaaaactct gtttagtgct
     3241 aatatttcca acatacttac catctacctc tattatgtcg tttttagaaa ttgaatttat
     3301 ctcttgacta ttcaaatcca aatatgcctt aaaggttttt ggaaaagtat tgatgttctt
     3361 agtataaggt aaatatgctt ttctaatatt gtcaatttgt ttgttgtttg caaatataat
     3421 caaacccggg ttgtaagtat ttgtagtctc tgggacataa gcctctttta aattttttac
     3481 aacgtcaaat ttatttccca atagatttat ttttttcaaa cggctatcac ctttttgaac
     3541 aaagtaagcc acttggttat cttttaattt tggtaattgg tgacctaaac gacggaaatc
     3601 attctgagta attaggtaca taaaacccgt ttttgtgata tcaacatgtt tgacgttttt
     3661 agatgtgata ttaagttcac ttgattgact aactggcatc gaaatcattg tctgattata
     3721 agtgatagct tccttagatg acttacctag tttcttcaaa atcttttctt caaatatatt
     3781 tttcgcgtca ccttttgaat tatctattga taaacttact gattttggaa atagtccggt
     3841 aacaacattt tgtgtatttg aatagagtga gactgttgta gcaatagtaa ctagagccat
     3901 aacagctaat aaagtgatac ttgctaaccc tactgcattt tgcttcattc gaaaaatcat
     3961 ttgcgaagtt gatacaaaat gctcagattt ataataataa tgcttgtttt gacgcaacct
     4021 ttttaagtac catgtcataa aactaatata aaaaagataa gtaccagcaa taactaaaag
     4081 tactgcaaag aagaaacgat agataacagc taatgcaggt gctttacctg aagtaagagc
     4141 cattgtataa gcgatggcga tagctactaa cgctaaaatt gcaagtataa gattaccttt
     4201 tggttctttt tctccctgtt gtttctttct aaaaagactt aatggggatg ataaatgaac
     4261 atgtcgaata actggaactt ctaaagtcag aaaaatacct gtaaatataa cgatacaaat
     4321 aataaatggc cataaactta aactaagatt tagtgcatga tagttaataa tgttgacaaa
     4381 aattaaataa ataaatttag caaaaaaagc actaaacaga cttcctatag aaataagaaa
     4441 tatataaata ataaacagct ctagactagc tacacgcgca acttgacgtt tattcatccc
     4501 caaaatgtta tacagtccaa attcactact acgctgtttc atcaagatat tgtagctata
     4561 atgttccatg actaccgcaa agattgacaa aacaatcact ccaagaaaaa gaaccgttgc
     4621 cgctgtcccc atatctcttc caacagcact cattaagatt agtagcgtag aacaagtcaa
     4681 tgaataaagt aataaactgg ctaagaggaa tggtatgtac tggtctatag aatgttttaa
     4741 attatgccag gctattttga gataaaacat attattcacc ccctaatatg gcagacattg
     4801 ttaaagaaat atctttattg aattcgctat tattcttatt accacgataa agttggtgaa
     4861 aaatacgtcc atctttgata aataaaacgc gtttggcatg actagcagca tttgctgaat
     4921 gggttaccat caaaatagtt tgtccatcca agttaatagt ttcaaataaa ttcagcaagt
     4981 cttctgaatt acggtaatct aacgctgctg ttggttcatc tgctaataaa atttgagggt
     5041 tggttatcaa actacgagca atcgctacac gttgcttttg cccaccagag agctcgaaag
     5101 gtctcttatc taataagtca tcaattctca gatgagaaga taattctgac aaacgatgat
     5161 ccatttcttt gtaacgttta cgatcaagaa ctaaaggaag atagatatta tctcttacag
     5221 agagagtatc caaaaggttg aagtcctgaa agacgaaacc aagatttttc aaacgaaaac
     5281 tcgctaattt tgcctcttta attttcgtaa tatcttcccc atttaaaatc acttgtccat
     5341 ttgtcggttt ttctaaagta gctaaaatat ttagcagagt tgtttttccc gaaccagact
     5401 ctcccataat agcaatgaat tccccttgtt caactttaaa atcgacgtct tgtagagccc
     5461 ttgtctcttc ttttgaaaaa cgtgtacgaa aaactttttc taagtgattg atttctaata
     5521 acatataatt ctcctttatt tattataccc taattttgat tgattctact ctttaaccaa
     5581 gactgtgact cttgtaaagg aaggctggtt acttgatctg gtaagatagg ggtgttctcg
     5641 tatttatatc cttgtcgatc actaactatt cctgtagtta aagctaatct agcaccgtcg
     5701 taaagcatga aagtttcatt aacagtcgta tatcctgctg tagcttgacc aaagctttta
     5761 acattaggta atcctttaaa tgataaaaaa gtcatttctg ccgaactagc agttttatga
     5821 ttagttataa tagcgattgg aacatgctta gtattgattg ttttcctaga tatttttaat
     5881 gcttctagcg gtatattttt catcgtgatt gttttcttat ttccatactt atcagtataa
     5941 tgaaataggg tatcatttgg taaaatagag gctaaccccc caatcatagg gatcatattg
     6001 ccgccagtat tattcgataa gtctaaaatg agtcccttat aacttttttc catcaatata
     6061 ttttgtaatt taccactata atggctaata gatcgtttat ctaacttact tatagaagga
     6121 atagtaatta agagaatgtc ttcgtttaat cttcgatatt ctggatatcg actatctagg
     6181 gtatctcttc gtacagtctc tttggataat atttgactat gtttaccgcc tccataccgt
     6241 actgcttcct gtacaatgtt gcgactctct tgataagatt ttgacgtttt caactttttt
     6301 tctgatcgtt gtcttataat ttgccattga cgaccttgcg aatacaaacc acgctgttca
     6361 acacgatcta aaataacacg tccataacgt tcgctactag gaggtaatag ataaatattt
     6421 aatgctgaac cataataatg aactaccact cctaaaattc ctaaaactat aagcattgga
     6481 actacaaaaa gtaaaactat ttttcgccgc atctcttgct ccccctttct ctttaagtct
     6541 attatatggc aaaatggttt actataacct tacatcttaa gacctatttc ttacaaattt
     6601 gaaagtttta aaagctttat aaactataat tttgatataa tatcttacat aacaaatgga
     6661 aagtacggtg aaaacatatg gccaagacaa gtaaaaaatt gacaaaatct ttagggccta
     6721 ttggtaaatt aatttcaata atccctgaca caactgaact tattggaaaa gcaattgata
     6781 attctcgtcc aattattgaa aaagaacttg accgtcgtca tgaaaaaaaa cagacttata
     6841 ctcagcttga taatgtagta caccttgata tcaatgatgc gcaagtttac ttagagcaac
     6901 gccattttaa tgtatcaagg attgttgctg aaccaaattc aaagtgggca accctacatc
     6961 ctaatcaagt tgtgaatatg tcgcctaaag ctggtagggt cagaatcggt tcattagtga
     7021 agttgtacta tattacagag gatatccttg aagaaagtaa gacactactt ttatctaaat
     7081 ccgaaaaaag taaagcacga caacaacggt taacagaagt attgagtacc cctagtcaat
     7141 tactcaaaaa aaatcgaaat aaataagcat gatcgaattg accatgctta tttattttta
     7201 ccaaacaggt tgatctaaac cttgagatgt tttttcgata acttttttaa ccgcatctgt
     7261 atggtaagct ttcacaactt ctttaatggc tttagctttt gatttgtctt gccaaccttt
     7321 ttgagcaact aaaaggttgt accattgttt tgcattttta ctttttggtt caataaaaat
     7381 agctgattta ggatttattc ctgcttctgt cacaaaatca ttattaataa ctgctgcatc
     7441 tgtggaatcc aaagaacgtg cggtctgtgc tgcatctact tcttttaaat ctaaagattt
     7501 aggattggaa acaacatctg acattgttgc taatgtatca cctgatactt tcaattttag
     7561 caagcctgct gattgtaaca agtagagaga gcgactttcg ttaactgcat cgtttgggat
     7621 agtaatagtt gctttatttg gaatttcaga aactgtttgg tatttacctt taccgttctt
     7681 agtaccagag tataatctaa aggaagtaaa gtatgtctca gcaacggaaa caagattggt
     7741 cttatttgct ttattccaat tgtttaagta attataatgc tggaaagcat taatatcaat
     7801 ctcatcactt tctaaagctt tattaggttg tgtataatct gtgaattccg taaattttag
     7861 cttaacgcct tttttcttaa ctaattcttc gattttatcc caacgagctt tttcagtttt
     7921 actctttacc attgttccaa ctctaacgac tttggcgtct tcatttttag actgaccaca
     7981 ggctcctaaa ataagtgctg aaattactac tgttgtcgtt aaccctaata gctttttgat
     8041 tttcataatt tatttctcct ttgatatggt tgtatgatta atcaactgat actagtgtaa
     8101 caaaaagcta atggattaaa aaatac
//
LOCUS       DATROD010000079         7751 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.079, whole genome shotgun sequence.
ACCESSION   DATROD010000079 DATROD010000000
VERSION     DATROD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7751
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.079"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(120..1355)
                     /locus_tag="VBC74_001543"
     CDS             complement(120..1355)
                     /locus_tag="VBC74_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO6942481.1"
                     /translation="MATSTENLQLAKRGPIISIIAYITLAVAKLAAGYWFDATSLVAD
                     GFNNLSDILGNVALLIGLHLASQPADSNHRFGHWKIEDLASLITSFIMFVVGIQVFIQ
                     TVTKIINNTDTNIDPLGAIVGAISALVMLGVCFYNKQLSQRVKSSALVAASKDNLSDA
                     VTSIGTSIAIIAASLNFPIIDRLAAIIITYFILKTAYDIFIESAFSLSDGFDDYQLKQ
                     YEKAILTIPKISAVKSQRGRTYGSNIYLDIVLEMNPDLSVFESHAITEKVEKLLSDKF
                     SVYDIDIHVEPASIPEDEIFDNVYQKLYKNEKIILAKIPGYETFISPDFYMINEKGNI
                     ITSDMLTNATNHSLASNFKYFNVKSISQKTKLVSYELEGKRHTSIWRRNEKWFLIYHQ
                     ITAKSSPYKTRRYQITSLD"
     gene            complement(1419..2765)
                     /locus_tag="VBC74_001544"
     CDS             complement(1419..2765)
                     /locus_tag="VBC74_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEO6942482.1"
                     /translation="MLTLFTHINSFVWGPPLLALLVGTGIYLSFRLGFVQLRQLSRAF
                     KLIFREDNGQGDISSYAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGLLAIKYRTKDTNGEISGGPMYYIVNGMGQKWKPLAVFFSAAGILVALLGIG
                     TFTQVNAIASSLEHTFKISTRFTSLILAVIVLFIIFGGIKSISKVSEKIVPFMAISYI
                     LATLIIIAVNYNKIPHTFQLIFSGAFSGTAAIGGFSGAIVKEAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTKEPVEQGLISMTGTFIDTIVICTLTGIAILVTGKWLEFDLQGAPL
                     TQASFNTVFGSLGSFALTFCLVLFAFTTILGWSYYGERCFEYLLGTKFINAYRIIFVI
                     MVGLGGFLQLDLIWVIADIVNGLMALPNLIALLALSPIIVKETQKYFSETKNITKP"
     regulatory      complement(2922..3054)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3362..4687
                     /locus_tag="VBC74_001545"
     CDS             3362..4687
                     /locus_tag="VBC74_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEO6942483.1"
                     /translation="MTLNAKGESNQILVDKEVHSTNLLLDIDEKPELFQGLLLSFQHV
                     FAMFGATILVPLILGMPVSVALFASGCGTLIYQVATKFKVPVYLGSSFAYITAMALAM
                     KQMHGDISAAQTGILFVGLIYVVVATVIKFVGNSWVDKILPPIIIGPMIIVIGLGLAN
                     SAVTNAGFVAKGDWRKMLVAVVTFLIAAFINTKGKGFIKIIPFLFAIIGGYILSIILG
                     LVDLSPVEKAAWFELPKFYLPFKTGLFHSYKLYFGPEMLAILPISIVTIAENIGDHTV
                     LGQICGRNFLKKPGLNRLLIGDGLATAFSALIGGPAETTYGENTGVIGMTRIASVTVI
                     RNAAFIAIAFSFFGKFTALISTIPSAVLGGMAILLYGVIASNGLKVLIENRVNFAEVR
                     NLIIASSMLVLGLGGAVLDLGALTLSGTALSAIVGIILNLILPKESMKS"
     gene            4820..5206
                     /locus_tag="VBC74_001546"
     CDS             4820..5206
                     /locus_tag="VBC74_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEO6942484.1"
                     /translation="MKFNLEQVKVFENYEIENWEEGQVTLTTKVVDSSLNYYGNAHGG
                     YLFTLCDQVSGLVAISTGYEAVTLQSNINYLRAGRLDDLLTVIGTCVHNGRTTKVVDV
                     HIKNQKDEMVTTAIFTMFVTGERKEK"
     gene            5312..7591
                     /gene="pcrA"
                     /locus_tag="VBC74_001547"
     CDS             5312..7591
                     /gene="pcrA"
                     /locus_tag="VBC74_001547"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEO6942485.1"
                     /translation="MNPLIIGMNDKQAEAVQTTDGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKYVNPWNILAITFTNKAAREMRERAIALNPATQDTLIATFHSMCVRILRREADYI
                     GYNRNFTIVDPGEQRTLMKRIIKQLNLDTKKWNERSILGTISNAKNDLLDEIAYEKQA
                     GDMYTQVIAKCYKAYQEELRRSEAMDFDDLIMMTLRLFDQNKDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPQAKVVL
                     LEENYRSTKKILQAANNVINHNKNRRPKKLWTQNDEGEQIVYHRANNEQEEAVFVAST
                     IDNIVREQGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDVIA
                     YLNILANTSDNISFERIVNEPKRGVGPGTLEKIRSFAYEQNMSLLDSSSNVMISPLKG
                     KAAQAVWDLANLILTLRSKLDSLTVTEITENLLDKTGYLEALQVQNTLESQARIENIE
                     EFLSVTKNFDDNPEITVEGETGLDRLSRFLNDLALIADTDDSATETAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRAIEDADELEEERRLAYVGITRAEQILFLTNANTRTLF
                     GKTSYNRPTRFIREIDDELIQHQGLARPVNSSFGVKYSKEQPTQFGQGMSLQQALQAR
                     KSNSQPQVTAQLQALNANNSHETSWEIGDVATHKKWGDGTVLEVSGSGKTQELKINFP
                     GIGLKKLLASVAPISKKEN"
BASE COUNT         2550 a         1254 c         1466 g         2481 t
ORIGIN      
        1 attttcaagg ctatgaaacg tttacataaa aacaaattaa aaagacggag attcttctca
       61 atctttttaa tttgtttttg taagctattt ttatcaagag taactgatta aatagtatat
      121 taatcaaggg aagtgatttg atatcgtctc gttttatacg gacttgactt ggcagttatt
      181 tgatggtaga ttaagaacca tttttcatta cggcgccaaa tactagtatg tcgtttacct
      241 tccaattcat aagacacaag ttttgttttt tgactaattg attttacatt gaaatattta
      301 aagttgcttg ccaatgaatg atttgtagca ttagtaagca tgtcagaagt gataatattt
      361 cccttttcat taatcatata aaagtcaggg ctaataaaag tctcataacc ggggattttt
      421 gctaggatga ttttttcgtt tttgtataat ttttggtaaa cattatcgaa aatttcgtct
      481 tctgggatag aagcgggttc tacatgaata tctatgtcat atacagaaaa tttatcactg
      541 agtagttttt ccactttttc ggtaatggca tgactttcaa agactgagag atcagggttc
      601 atttcgagaa caatatcaag gtaaatatta ctaccgtaag tacgtccacg ttgagatttt
      661 acagcggata ttttaggaat tgttaatata gctttttcgt attgttttaa ttgataatca
      721 tcgaaaccat ccgataagct gaaagcagat tctataaaaa tatcataagc tgttttaaga
      781 ataaaataag taataatgat agcagctaaa cgatcaataa taggaaaatt tagagaagcc
      841 gcaatgatag caatagatgt gccgatagat gtcacagcat cagaaaggtt atctttagaa
      901 gctgcaacca gagcgctaga ctttactctt tgagataatt gtttattata gaaacaaacg
      961 ccaagcataa caagagcaga tatagcacct actatagcac ctagaggatc aatgttagta
     1021 tcagtattat taataatctt agtgacagtt tgaataaaaa cttgaatacc tacgacaaac
     1081 atgataaaag aggtaatcag gcttgctaaa tcttcaattt tccagtgacc aaaacgatgg
     1141 tttgagtcgg ctggttgact agccaagtgt aaaccaatta agagtgctac atttcctaag
     1201 atatcagata agttattaaa tccatctgct acaagtgaag tagcatcaaa ccaataacct
     1261 gcagctaatt tagcgactgc cagtgttata taggcaatga tactaataat aggccctctt
     1321 tttgcaagtt gtaagttttc agtagaagtt gccattttcc taatttcctt tcatctacac
     1381 attatatcac ttaggagcag actataaaac tagtaagttc aaggcttagt gatattttta
     1441 gtctctgaaa aatatttctg tgtttcttta acaataattg gtgacaaagc taggagagct
     1501 attagatttg gtaaagccat taaaccatta acgatatcag cgataaccca aatgagatcg
     1561 agctgtaaga aaccacctaa gccgaccata atgacaaaaa taatgcgata agcgttaata
     1621 aatttagtcc ccaatagata ttcaaagcaa cgctctccgt agtagctcca tcctaaaata
     1681 gtagtaaaag caaataagac aaggcagaag gttagggcaa aactacctag tgagccaaat
     1741 acggtattaa aagatgcttg tgttaacgga gcaccctgta aatcaaattc tagccatttt
     1801 cctgtaacta aaattgcaat ccctgtcaaa gtacaaataa caatagtgtc aatgaaggtt
     1861 cctgtcattg aaataagccc ttgttcaaca ggttcttttg ttttagctgc tgctgcagct
     1921 attggagcag aacccaatcc agattcattt gagaaaactc ctctagcaat tcccttttga
     1981 attgcttctt taacgatagc tccagaaaaa ccaccaatgg ctgctgtccc agaaaaagca
     2041 ccgctaaaga tgagttggaa agtatgtgga atcttattgt aattaactgc aataataatc
     2101 agagttgcta aaatatagct tatagccata aaaggcacta ttttttctga aacttttgaa
     2161 atagatttta tacctccaaa tataataaat aatactataa cagcaagaat gaggctggta
     2221 aatctagttg atattttgaa agtatgctca agagaagagg caattgcatt gacttgagtg
     2281 aatgtcccta ttcctaaaag agctactaag atacctgctg cagaaaaaaa gactgctaat
     2341 ggtttccatt tttgtcccat tccattgaca atgtagtaca ttggtccccc agaaatttct
     2401 ccattagtat cttttgtgcg atatttgata gctaataatc cttctgcgta ttttgttgcc
     2461 attccaaaaa aggcggctac ccacatccaa aacaaagctc ctggtcctcc agatttaata
     2521 gccgtagcca caccaacgat attacctgtc cctaccgttg cagcaagagc agttgcaaga
     2581 gcagcataac ttgaaatatc tccttgtcca ttatcttctc ggaaaatcaa tttgaaagct
     2641 ctagaaagtt gtctcaattg aacaaaacct aagcgaaatg ataggtaaat acctgttccg
     2701 actaataaag caagtaaagg tggaccccaa acgaagctat tgatatgagt aaaaagtgtt
     2761 agcatatgtt ctcctttgat acatgacata cctatggatt ctgtaatgaa acgaaagaag
     2821 ttatctctaa aagagataac ttctgaaaga tgctagctaa aaagcgcaca aataaaggta
     2881 gtagtaaact taataagctt actactcatt attaatattg gcttttagct tctgtccttt
     2941 tgcctgagag attggaggag ttgttttact cttcttgccc cttcggcgcc tagagcacat
     3001 cttggtgtca atacaaagat gttgctactt ataggtctct ccagaagtcc gtctgtcaat
     3061 cagtcctgtc ttaagacacc tgagagtgta attccttcgg cgaaagtcta actttcttct
     3121 cctgaatgac gtcattcggt atattttatt atgttaacta tcataatgaa aaagcatagt
     3181 tttgtcaata tagtttcaaa aaactcttgt tttctaaaat gtcttatgct ataattcttt
     3241 cagcgtaggt ttttaaactc atcccgtgag gtgagaaaaa catcaggaaa aaaataaagc
     3301 ctatgactat attgctatag aaagtgggct tttttgtatg cccaagagga ctagtttaga
     3361 tatgacttta aatgctaaag gtgaaagtaa tcaaattcta gtagataagg aagtacattc
     3421 tactaactta ttactagata tcgatgaaaa accagaatta tttcaaggtt tgcttttaag
     3481 ttttcaacat gtttttgcca tgtttggagc tacgatatta gttccgctta ttttagggat
     3541 gcctgtatca gtggctttat ttgcttctgg ttgcggaacg ctcatttatc aagttgcgac
     3601 aaaatttaaa gtccctgttt atctaggctc atcatttgct tatattacag ctatggcatt
     3661 agccatgaaa caaatgcatg gcgatatttc ggctgctcaa acaggtattc tttttgtagg
     3721 tctaatctat gtcgttgtgg cgacggttat taagttcgta ggaaattcat gggttgataa
     3781 gattttgcca ccaataatca ttggacctat gattattgtt attggtttag gtttggcaaa
     3841 ctctgctgta actaatgcag gttttgtcgc taaaggtgat tggcgtaaaa tgctagttgc
     3901 tgttgttaca ttcttgattg cagcttttat caatactaaa gggaaaggtt ttatcaagat
     3961 tattccattt ttatttgcaa ttattggagg ttatatttta tcaatcattc ttggcttggt
     4021 agatttatct ccagttgaaa aagctgcctg gtttgaatta ccaaaattct atttaccgtt
     4081 taagacaggt ttatttcaca gttataaact ttactttgga ccagaaatgc ttgcaattct
     4141 tccaatctca attgtaacga ttgctgaaaa tatcggtgat catacagttt taggtcagat
     4201 ttgtgggcgt aactttctaa aaaaaccagg tttaaatcgt cttttaattg gagatggttt
     4261 agcaaccgca ttttctgctc ttataggagg gccagctgaa actacttatg gtgaaaatac
     4321 aggtgttatt ggcatgacgc gtatagcttc tgtaacggtt attcgtaatg cagcttttat
     4381 tgcaatcgct ttttcattct ttggtaaatt tacagctttg atctctacaa ttcctagtgc
     4441 agtacttggt ggaatggcta tattacttta tggtgttatt gcaagtaatg gattaaaagt
     4501 gttgattgaa aatcgtgtca attttgctga ggttcgtaat ctgattattg ccagttccat
     4561 gttagtttta ggcctcggtg gagcagtcct tgatctaggt gctctaactt tatcaggaac
     4621 agcattaagt gctatagtgg gtattatatt aaatttaata ttgccaaaag aaagcatgaa
     4681 atcataacaa aagagctcag agtatctgag ctcttttgtt atgatagatc agttaaaaac
     4741 tttaaaataa aaacgtttat agttatggtt tccttatttt ctgctataat ggtcatagaa
     4801 aaagtgaggt tatagtagaa tgaaattcaa tttagaacaa gtaaaagtat ttgagaatta
     4861 cgaaattgaa aactgggaag aaggtcaagt taccttaaca acgaaggtcg ttgactcttc
     4921 gctcaactat tatggtaatg ctcatggtgg ttatttgttt acactgtgtg atcaagtcag
     4981 tggtttagtt gctatttcaa caggatatga agcggtaact ctacaatcaa atatcaacta
     5041 tttgagggca ggccgattag atgatctgct tactgtcatc ggtacttgtg ttcataatgg
     5101 tcgtactaca aaagtcgttg atgttcacat taaaaatcag aaagacgaaa tggttacaac
     5161 tgctattttt acaatgtttg taactggaga gagaaaagag aagtaaaaaa ggtaggagtt
     5221 agacagcgtc tgtttaactc ctttttctat tgggaaaaag atgaaaaaat gatatactag
     5281 agaggcaatt ttgacaaaat taggagtaga aatgaatcct ttaataattg gaatgaatga
     5341 taaacaagca gaagcggtac aaacgacaga cggaccgctt ttgattatgg caggagctgg
     5401 ctctggaaaa acacgtgttc tgactcatcg tattgcttat ttaatagatg aaaaatatgt
     5461 taatccttgg aatattttag cgattacttt tactaataaa gcagcgcgtg aaatgcgtga
     5521 acgtgctatc gccttgaatc cagctaccca agatactttg attgcaactt ttcatagtat
     5581 gtgtgttcgt attttacgtc gtgaagctga ttatattgga tataatcgta actttacaat
     5641 cgttgatcca ggtgagcagc gtactttaat gaagcgaatc attaagcaat taaatttgga
     5701 cacaaaaaag tggaatgaac gttcaatttt aggtacaatc tctaatgcta aaaatgacct
     5761 tcttgatgaa attgcatatg agaagcaagc cggagatatg tatacacagg taattgctaa
     5821 atgctataaa gcttatcaag aggaattacg tagaagtgag gccatggatt tcgatgactt
     5881 gattatgatg acacttcgat tatttgatca gaataaagat gttttggcct attaccaaca
     5941 gaggtatcaa tatatccatg tagacgagta tcaagatact aaccacgctc aataccaatt
     6001 agttaagtta ttagcttcgc gtttcaaaaa tatttgtgtt gttggtgatg ccgatcaatc
     6061 catttatgga tggcgtggag ctgatatgca aaatattctt gattttgaaa aggactatcc
     6121 gcaagccaaa gttgtattat tagaagaaaa ttatcgatca actaagaaaa tacttcaagc
     6181 tgctaataat gtgatcaatc ataataaaaa tcgccgtcct aaaaaattat ggactcaaaa
     6241 tgatgaaggt gagcaaattg tatatcatag agctaacaac gagcaagaag aagccgtttt
     6301 tgtagcgtca actattgata acatcgttcg agaacaagga aaaaatttca aagattttgc
     6361 cgttctttat cgtacgaatg cgcaatctcg tactattgag gaagcacttt taaaatccaa
     6421 tattccatat acaatggttg gtggaacaaa attctatagt cgtaaagaaa ttcgagatgt
     6481 tattgcttat ctcaatatcc ttgcaaatac ttctgataat atttcttttg agcggattgt
     6541 aaatgaacct aaaagagggg tggggccagg aactttagaa aaaatacggt catttgccta
     6601 tgaacagaac atgtctcttc ttgattcctc ttcaaatgtc atgatatcgc ccttaaaagg
     6661 gaaggctgct caagctgttt gggatttagc taatctgatt ttgactctac gtagtaagct
     6721 agatagttta actgtaacgg agattacaga aaacctactg gataagacag gatatctaga
     6781 agcacttcaa gttcaaaata cattagaaag tcaagcgcgt attgaaaata ttgaagaatt
     6841 cttatcagtg actaaaaatt ttgacgataa cccagagatt acagtagagg gtgaaactgg
     6901 tttagatcgt ctatcgcgtt ttttaaatga tttagcttta atagcagata ctgatgatag
     6961 cgccactgaa acggctgaag tcactttaat gacattacat gctgccaagg gtctagaatt
     7021 tccagttgtt ttcttaatcg gtatggaaga aggagtattt cctttatcgc gtgcaataga
     7081 ggatgctgat gaattagaag aagagcgtcg tttagcttat gtcgggatta cgagagcaga
     7141 acaaatactt ttccttacca atgctaatac ccgtacttta tttggtaaaa ctagttataa
     7201 tagaccaacc cgttttatac gtgagattga tgatgagctg atacaacatc aagggttggc
     7261 tcgaccagtt aattcttcat ttggtgtgaa atattcaaaa gaacagccta cacaatttgg
     7321 tcaaggaatg agtcttcaac aagcgcttca ggcacgtaaa agtaattcac aacctcaagt
     7381 aactgcccaa ctccaggctc ttaacgcgaa caatagccat gagacatcat gggagattgg
     7441 tgatgttgca actcataaaa aatgggggga cggtacggtg cttgaagttt caggtagcgg
     7501 aaagactcaa gagttaaaaa taaattttcc aggtattggg ttaaagaaat tattagcgag
     7561 tgtcgctcct attagtaaaa aggaaaacta actttcttct attatatgct taaataatca
     7621 gagactaaga attgtattct tagtcttttt gcatcatttg aatagacaaa ataagttatc
     7681 atttggtttt tacttactaa aaaggttgat attaaagcgt tttccaaaag tgaccatact
     7741 attttaaaga a
//
LOCUS       DATROD010000080         7651 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.080, whole genome shotgun sequence.
ACCESSION   DATROD010000080 DATROD010000000
VERSION     DATROD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7651
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.080"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(27..2822)
                     /gene="ppc"
                     /locus_tag="VBC74_001548"
     CDS             complement(27..2822)
                     /gene="ppc"
                     /locus_tag="VBC74_001548"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEO6942486.1"
                     /translation="MSHPKLESSSNKEIITEEVGLLKQLLDEATQKLIGSESFDKIEK
                     IVSLSSTDDYTGLKETISALSNEEMVIVSRYFSILPLLINISEDVDLAYEINYKNNLN
                     QDYLGKLSTTIDVVAGHENAKDILEHVNVVPVLTAHPTQVQRKTVLELTSKIHDLLRK
                     YRDVKAGIVNQEKWYADLRRYIGIIMQTDTIREKKLKVKNEITNVMEYYNRSLIKAVT
                     KLTAEYKALAAKKGIHLENPKPLTMGMWIGGDRDGNPFVTAETLRLSAMVQSEVIINH
                     YIEQLNELYRNMSLSISLTEVSPELVTLANQSQDNSVYRENEPYRKAFNFIQDKLVQT
                     LLNLKVGSYPKEKFVSRQESSGIVGRYIKSHIAQVASDIQTEELPAYATAEEFKQDLL
                     LVKQSLVQHGQDSLVDGELACLIQAVDIFGFYLATIDMRQDSSINEACVAELLKSANI
                     VDDYSSLSEEEKCQLLLKELTEDPRTLSSTHTPKSELLQKELAIFQTARELKDQLGED
                     IIKQHIISHTESVSDMFELAIMLKEVGLIDANQARIQIVPLFETIEDLDNSRDIMTQY
                     LHYGLVKKWITTNNNYQEIMLGYSDSNKDGGYLSSGWTLYKAQNELTKIGEENGIKIT
                     FFHGRGGTVGRGGGPSYEAITSQPFGSIKDRIRLTEQGEIIENKYGNQDAAYYNLEML
                     ISASIDRMVTRMITNLNEIDNFRETMDGIVAESNAVYRNLVFDNPYFYDYFFEASPIK
                     EVSSLNIGSRPAARKTITEISGLRAIPWVFSWSQNRIMFPGWYGVGSAFKQFIEQDEA
                     NLAKLQTMYQKWPFFNSLLSNVDMVLSKSNMNIALQYAQLAGSKEVRDVFNIILNEWQ
                     LTKDMILAIEQHDNLLEENPMLHASLDYRLPYFNVLNYVQIELIKRLRSNQLDEDYEK
                     LIHITINGIATGLRNSG"
     gene            3031..4830
                     /gene="pepF"
                     /locus_tag="VBC74_001549"
     CDS             3031..4830
                     /gene="pepF"
                     /locus_tag="VBC74_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEO6942487.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEKALEDIKVFKKN
                     YEGKLNCVEDFTSALMEIEHIYIQMSHIDTYAFMPQTTDFSNEEFAQISQAGSDFATK
                     ANVLLSFFNTALANADIKILDSLENNPHFKATIRQAKIQKQHLLSPEVEKALTNLNEV
                     LNTPYDIYTKMRAGDFDMEDFEVDGKTYKNSFVTYENYFQNHENAEIREKSFRSFSKG
                     LRKHQNAAAAAYLAKVKSEKLIADMRGYDSVFDYLLSEQEVDRSMFDRQIDLIMDEFG
                     PVAQRFLKHIADVNGIEKMTFADWKLDIDNELNPEVSINDAYDLVMKSVAPLGKEYSQ
                     EVERYQKERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSFFNTHMSTYYVEAPSTFNELLLSDYLENQFDTARQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGAEQLNAIMKEVLTQFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLNLKNNPNGAKEWLAFLKSGGSCTPLETALLIG
                     ADISTDKPLRDTINFLSNTVDQIINYSNLIKQG"
     gene            complement(4889..5278)
                     /locus_tag="VBC74_001550"
     CDS             complement(4889..5278)
                     /locus_tag="VBC74_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEO6942488.1"
                     /translation="MKKYIKLFLLTVFATTLVACGQPSTSNKTTTSSTLEVGKVELVV
                     KEDTNVLSEKVVYHKGDTVLDVLKANYKVKEKDGFITSIDGISQDETKGLYWMFKVNN
                     KLAPKAANQIKVKKNDKIEFYQEVYKK"
     gene            complement(5262..5786)
                     /locus_tag="VBC74_001551"
     CDS             complement(5262..5786)
                     /locus_tag="VBC74_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6942489.1"
                     /translation="MIKNSTLTKLTRVAILSALCVVLRYAFAPLPNIQPITAIFLITV
                     VLFDLKEGVATVTVTMLVSSFLMGFGPWVFLQIISFTLILCLWKFLIYPLTKAVCFGK
                     ITEVVLQTFFAGGLGVVYGVIIDTCFAWLYHMPWWTYVLAGLSFNMAHALSTCLFYPL
                     LLPILRRFRNEKIH"
     gene            5943..6791
                     /locus_tag="VBC74_001552"
     CDS             5943..6791
                     /locus_tag="VBC74_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEO6942490.1"
                     /translation="MRRRIKPIVVAVFFSLFGLLLIIGHLHSTNTLKKELVEAKKTIP
                     SVKASKVPQKSTSSKDKEFVLKPIIDVSGWQLPKEIDYDTLSKNISGVVIRIFGGSKI
                     SKTNNAAYTTGIDKSFKTHIKEFQKRNIPVAVYSYALGSSVKEMKEEAQIFYKNAAPY
                     KPTFYWIDVEEETMSNMNKGVQAFRKELKRLGAKNVGIYIGTYFMTEQGISVKGFDAV
                     WIPTYGSDSGYYEAAPQTELKYDLHQYTSQGYLPGFNQPLDLNQIAVNKDKKKTYEKL
                     FGKVKD"
     gene            complement(6824..7441)
                     /locus_tag="VBC74_001553"
     CDS             complement(6824..7441)
                     /locus_tag="VBC74_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000404311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEO6942491.1"
                     /translation="MEILIAGGSGFLGKQIIKAALTKGHKVAYLSRHEGKGDIFKDPR
                     LTYIKGDITEADKIHLEHRNFDILIDCIGAIKPNQLDELNVKATQKAVALCHKNQIPK
                     LVYISANSGYSAYIKSKRKAEQIIKASGLDYLFVRPGLMYGEERPLSIFQAKCIKLFS
                     HLPFLGIVVQKVFPTKVVIVAEAIVTSLRKKPTQKILSIEELNNK"
BASE COUNT         2501 a         1395 c         1208 g         2547 t
ORIGIN      
        1 tagcaatgat cctccctcta tcaatattaa cctgaatttc gtaaacctgt tgcaatacca
       61 ttaatagtaa tatgaatcaa cttctcatag tcttcatcta gttggttact acgaagacgt
      121 ttaatcaatt caatttgaac ataattcaaa acattgaaat atggcaaacg ataatctaaa
      181 cttgcatgta acattgggtt ttcttctaat aaattatcat gctgttcaat agctaaaatc
      241 atatctttag ttaactgcca ttcattaagg atgatattaa aaacatctcg aacttcttta
      301 cttccagcta actgagcata ctgaagcgca atattcatat ttgattttga taaaaccata
      361 tctacattag ataaaagaga attaaagaat ggccattttt gatacatggt ttgtaatttt
      421 gctaagtttg cctcatcttg ttcaataaat tgcttgaagg ctgacccaac accataccaa
      481 cccggaaaca taatacgatt ttgtgaccat gaaaataccc atggaattgc acgtaaacct
      541 gaaatttctg taattgtttt acgtgctgct ggccgagaac caatatttaa acttgatacc
      601 tctttaattg gacttgcttc aaagaaatag tcatagaaat aaggattatc aaaaactaaa
      661 tttctataaa cggcattact ttcagccaca ataccatcca ttgtctcacg gaaattatca
      721 atttcattaa ggttagtaat catacgtgtt accatgcggt ctatggaagc tgaaataagc
      781 atttcaaggt tataataggc tgcatcttga ttaccatatt tattctcaat gatttcacct
      841 tgctctgtta ggcgaattcg atctttgatt gaaccaaaag gttgtgatgt gatagcttca
      901 taagatggac caccaccacg accaacagtt ccaccacgac cgtggaaaaa tgtgatttta
      961 ataccgtttt cttcacctat ttttgttagc tcattttgag ctttatatag agtccaacca
     1021 gatgataaat agccaccatc tttattacta tctgaataac ctagcattat ttcttgataa
     1081 ttgttgttag ttgtaatcca tttttttact aatccataat gtagatactg ggtcataata
     1141 tcacgtgaat tatccaaatc ttcgatggtt tcaaataacg gtacaatctg aatgcgagct
     1201 tgattggcat caattagtcc aacttctttt aacatgatag ctaactcaaa catatccgaa
     1261 acgctctctg tgtgagaaat gatatgctgt tttataatgt cttcacccaa ttgatctttt
     1321 aactcacgtg ctgtttggaa aatagccaat tctttttgaa gtaactcgga tttaggagta
     1381 tgcgtagaag ataatgttct aggatcttct gttaattctt ttaagagaag ttgacatttt
     1441 tcttcttctg aaagagaact gtaatcatct acaatattgg cagacttcaa taattctgct
     1501 acacaagctt cattaatact agaatcttga cgcatatcaa tagtagctaa ataaaatcca
     1561 aagatatcta ctgcttgaat aagacaagct aattcgccat cgacaagaga atcttgtcca
     1621 tgttgaacta aagattgctt cacaagtaac aaatcttgtt taaactcctc agcagtagcg
     1681 tatgctggta gttcttctgt ctgtatgtca gaagctactt gagcgatatg tgattttata
     1741 taccttccta caataccaga agattcttgg cgactaacaa atttctcttt tgggtaagaa
     1801 ccaactttta aatttagtag ggtttgtact agtttatctt ggataaaatt gaaagcttta
     1861 cgataaggtt cattttccct atatactgaa ttatcttgtg actggtttgc caacgtaaca
     1921 agttcgggag agacttcagt tagacttatt gaaagtgaca tattacgata aagttcattt
     1981 agctgctcaa tataatggtt aataatcact tcactttgta ccatagctga taatcttagt
     2041 gtctcagcag ttacaaaggg attgccatct ctatcaccac caatccacat ccccatagtc
     2101 agaggcttag gattctctaa gtgaatacct tttttagctg ccagtgcctt atattctgct
     2161 gttaatttgg taactgcttt aataagagaa cggttgtaat attccattac attggttatc
     2221 tcatttttga ctttcaattt tttctcgcga atagtgtctg tttgcatgat aatgccgatg
     2281 taacgacgca agtcagcata ccatttttct tgattcacga ttcctgcttt gacatctctg
     2341 tatttacgta ataaatcatg aatcttagac gttaattcga gcaccgtttt acgctgcacc
     2401 tgagtaggat gagctgttag cacaggcaca acattgacgt gttcaagaat atctttagca
     2461 ttttcatggc cagctacaac atcaatagtc gttgataact ttcctaggta atcttggtta
     2521 agattatttt tataattaat ttcatatgct aaatcaacgt cttctgaaat attaattaag
     2581 agcggcaaaa tagagaagta acgagaaaca ataaccatct cctcgttgct taaagccgaa
     2641 atagtttcct tcaaaccagt ataatcgtca gttgatgata atgaaactat tttttcaatc
     2701 ttgtcaaatg attcactacc aatcaatttt tgcgttgctt catccaaaag ctgcttcagt
     2761 aaaccgacct cttcggtaat gatttcttta ttgctactac tttctaattt cgggtgggac
     2821 aaaccatcac ttcctctctt aaatctcact actatgatac cattttaaag taaaaagtaa
     2881 accattctag gatttttaga tagaattttc agatttttac ttatttatga cagtaatcag
     2941 tttttcttgt tcgacttttt tgatatactg aggataattg agattaactg attaattgtt
     3001 aattaacaac tattattaaa ggagtatcgc atggaactta aaaaacgctc tgaattccct
     3061 gaaaatgagc tttgggatct gactgctctc tacaaagatc gtcaagattt tttactggct
     3121 attgaaaaag ctcttgagga tatcaaggtt ttcaaaaaaa attacgaggg aaaacttaat
     3181 tgtgttgagg attttactag tgcccttatg gaaatcgagc atatttatat ccagatgagt
     3241 cacattgata cctatgcttt tatgcctcaa actactgact ttagcaacga agagtttgca
     3301 caaatttctc aagcaggatc agacttcgca actaaagcta atgtactact ttctttcttt
     3361 aatactgccc tagcaaatgc agatataaag attttagatt ctttagaaaa taaccctcat
     3421 ttcaaagcaa ctattcgcca agctaaaatt caaaagcaac acttattaag tccagaagtc
     3481 gaaaaagctt tgacaaactt aaatgaagtc ctaaatacac catatgatat ttatactaaa
     3541 atgcgtgctg gcgattttga tatggaagat tttgaagttg acggaaaaac ctacaaaaat
     3601 agttttgtaa cttatgaaaa ttactttcaa aatcacgaaa atgcagagat acgtgaaaaa
     3661 tccttccgtt cattctcgaa aggtctacgt aagcaccaaa atgctgcagc tgcggcttat
     3721 cttgcaaaag tcaaatctga aaagttaatt gctgatatgc gtggttacga tagtgttttt
     3781 gattatcttc tttccgaaca agaagttgac cgttcaatgt ttgacagaca aattgatctt
     3841 atcatggatg aatttggtcc agtagctcaa cgattcctca aacatatcgc cgatgttaat
     3901 ggtattgaaa agatgacttt tgcagattgg aaattagaca ttgataacga acttaatccc
     3961 gaagtaagta tcaatgatgc ttatgactta gtaatgaaat cagttgctcc tttaggaaaa
     4021 gaatattccc aagaggttga gcgctatcaa aaagagcgtt gggttgattt tgcagctaac
     4081 gctaataaag attctggagg atatgctgcg gatccttaca aagttcatcc ctatgtactc
     4141 atgagctgga cggggcgtat gagcgatgtc tacacactta ttcatgagat tggacattct
     4201 ggtcaattta tcttctcaga taatcaccaa agttttttca atactcacat gtccacttac
     4261 tatgttgaag ctccatctac ttttaacgaa cttttactta gtgactactt agaaaaccaa
     4321 tttgatacag cacgtcaaaa acgctttgct cttgcacatc gcttaactga tacttacttc
     4381 cacaatttca ttactcactt acttgaagct gctttccaac gtaaagttta cactctaatt
     4441 gaggagggag aaacttttgg agcagaacaa ttaaatgcta ttatgaagga agtattaaca
     4501 caattctggg gagatgctat tgaaatagat gacgatgctg ctttgacatg gatgagacaa
     4561 gctcattact acatggggct ctatagctat acttactctg ctggtttagt aatctcgaca
     4621 gccggctatc tcaatctcaa aaataatcca aacggagcaa aagagtggtt agcattcttg
     4681 aaatctggtg ggagctgtac ccctttagaa acagccctat taatcggtgc tgatatttct
     4741 actgataagc ctctacgcga tactattaat ttcttatcta acactgtaga tcaaattatt
     4801 aattatagta atcttattaa acaaggctaa actacagtct ttcaaaaaca taaaaacaag
     4861 ttggagatat ctccaacttg tttttatctt attttttgta aacttcctga taaaattcaa
     4921 ttttgtcgtt ctttttaact ttaatttggt ttgctgcctt aggagctaat ttattattga
     4981 ccttgaacat ccaataaagc cctttggttt catcttggct gatgccatca attgaagtaa
     5041 tgaaaccatc tttttcttta actttatagt tagctttaag cacatccaaa acagtgtcac
     5101 ctttatggta aacaaccttt tcacttaaca cgttcgtatc ttctttaaca accaattcaa
     5161 ccttaccaac ttctaacgtt gagctagttg ttgtcttatt cgatgtagat ggttgcccgc
     5221 aagctactaa agtcgtagcg aatactgtta gcaagaataa tttaatgtat tttttcattc
     5281 ctaaatctcc ttaaaatcgg taacaaaaga ggataaaata aacaagttga taaagcgtgt
     5341 gccatattaa aactaagtcc tgctaataca taagtccacc aaggcatatg atataaccaa
     5401 gcaaaacagg tatcaataat gacgccataa acaaccccta accctcctgc aaagaaagtt
     5461 tgaagtacta cctccgttat tttaccaaaa catacagctt ttgtcagcgg atatatcaga
     5521 aatttccaca agcataaaat taaagtgaag gaaatgattt gcaaaaagac ccatggccca
     5581 aagcccatta aaaaagaaga aactagcatt gttacagtta cagtagctac accttctttt
     5641 aaatcgaata agacaactgt aatcaaaaaa atggcagtaa ttggctgaat gttaggcaga
     5701 ggcgcaaaag catatctcaa cacaacacac aaagcagata atatagctac tctagtcaat
     5761 ttagttaaag ttgaattctt tatcatgtct ttaattatac ctcaaaacta tgactaccgc
     5821 ttaaaagtct tttttattac aatttcattt ctaatgttac ataaacgacc tcttttttta
     5881 aaagaacgta aataagcctg taaactttga ttttttaaac cttttaagtt acaatatcaa
     5941 taatgagaag aagaattaaa cctattgttg ttgcggtttt tttcagttta tttggacttt
     6001 tattaattat tggtcattta cattcgacaa atactttgaa aaaagaatta gttgaagcta
     6061 aaaagacaat tccatccgta aaagcttcaa aagtaccgca aaaatcaaca tcatcgaaag
     6121 ataaagagtt tgttcttaaa ccgattatcg atgtctctgg ttggcaactt cctaaggaga
     6181 ttgattacga tacgctttca aaaaatattt caggtgttgt tattcgtatc tttggtggat
     6241 caaagatatc taagactaat aacgctgctt atacaactgg aatcgataaa tcgtttaaga
     6301 cccatatcaa agaatttcaa aagcgaaata tcccagtagc tgtctacagt tatgcacttg
     6361 gttcaagtgt taaagaaatg aaagaagagg ctcagatatt ttataagaat gcagctcctt
     6421 acaaaccaac tttttattgg attgacgtag aagaggagac aatgtctaac atgaataaag
     6481 gtgtccaagc attccgaaaa gagttaaaaa gacttggtgc taaaaatgtt ggtatctaca
     6541 tcggtactta ctttatgact gagcaaggta tctctgtaaa aggatttgac gctgtttgga
     6601 ttccaactta tggtagcgat tctggatact atgaagcagc tccacaaact gaacttaaat
     6661 acgatttaca ccaatacacc tctcaaggtt atctaccagg attcaatcaa ccgcttgatt
     6721 taaatcaaat tgcagttaat aaagacaaga agaaaactta tgagaaactt tttggaaaag
     6781 taaaagatta aaagctactg ttaacagtag cttttttgat aaactattta ttatttaatt
     6841 cttcaataga aaggattttt tgagttggtt ttttcctaag cgaagtaacg attgcttctg
     6901 ccactatcac aaccttagtt ggaaagactt tttgtacaac aatacctaag aaaggcaaat
     6961 gactaaataa ttttatacac ttggcttgga aaatcgagag aggtcgctct tcaccataca
     7021 tcaaacctgg tcttacaaaa agataatcca gaccgcttgc tttgattatc tgctctgcct
     7081 tccttttact tttaatgtaa gctgaatagc cgctattggc tgaaatgtaa actaactttg
     7141 gtatttgatt tttgtgacag agtgctactg ctttttgggt tgctttaacg ttaagctcat
     7201 ctagttgatt gggcttaatc gctccaatac agtcaattaa tatatcaaaa tttctatgtt
     7261 ctaaatgaat cttatcagct tctgtaatat ctcccttaat gtaggttaat ctaggatcct
     7321 taaatatatc acctttacct tcatgccttg ataagtaagc caccttatgc ccttttgtaa
     7381 gcgctgcttt tattatctgc ttccctagaa aaccactacc acctgcaatc agtatttcca
     7441 tagttccctc tccttcattt tgtaaattta gtatagaaat ttttatatgc cttgtcaaag
     7501 taatcgttta atacctgacc agaatatgat ttcaagtcat tacattagga cacttcatat
     7561 ttagctattt ataaaacttc taatttaaca ttccaaatat actgttgcga tatttattat
     7621 cattattccc tgaaatggta ttcaagctta t
//
LOCUS       DATROD010000081         7524 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.081, whole genome shotgun sequence.
ACCESSION   DATROD010000081 DATROD010000000
VERSION     DATROD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7524
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.081"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            287..1297
                     /gene="nrdF"
                     /locus_tag="VBC74_001554"
     CDS             287..1297
                     /gene="nrdF"
                     /locus_tag="VBC74_001554"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO6942492.1"
                     /translation="MQSYYDRSQSPLDYALSEKAFPMRSVNWNKLNDDKDLEVWNRVT
                     QNFWLPEKIPVSNDLNSWRTLDADWQQLITRTFTGLTLLDSVQATVGDIAQIKHSQTD
                     HEQVIYANFAFMVAIHARSYGTIFSTLCTSQQIEEAHEWVVDTESLQARSRILIPFYT
                     GDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGY
                     KYQQKVAKLSVEKQAEMKTFVFDLLYQLIDLEKAYLYELYDGFDLAEDAIRFSIYNAG
                     KFLQNLGYDSPFTEEETRISPEVFAQLSARADENHDFFSGNGSSYIMGITEETLDEDW
                     EF"
     gene            1298..1711
                     /gene="nrdI"
                     /locus_tag="VBC74_001555"
     CDS             1298..1711
                     /gene="nrdI"
                     /locus_tag="VBC74_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEO6942493.1"
                     /translation="MDSTVKVVYFSSKSNNTHRFVQKLACSNQRIPSDGSSILVTEDY
                     ILIVPTYAGGGDDTKGAVPKQVVQFLNVRQNREHCQGVISSGNTNFGDTYAIAGPIIA
                     RKLNVPLLHQFELLGTQEDVTRVKELLCQFTRKDK"
     gene            1713..3878
                     /gene="nrdE"
                     /locus_tag="VBC74_001556"
     CDS             1713..3878
                     /gene="nrdE"
                     /locus_tag="VBC74_001556"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO6942494.1"
                     /translation="MTQSDAYLSLNAKTRFRDRTGNYHFTSDKEAVEQYMIEHVEPNT
                     MVFTSLIEKLDYLVSNNYYESDLLKQYNLEFICQIFEHAYAKKFAFLNFMGALKFYNA
                     YALKTEDNRYYLEHYEDRVVMNALFLAAGDEKAAYDLVDDMLANRFQPATPTFLNAGK
                     KRRGEYISCYLLRIEDNMESISRAISTSLQLSKRGGGVALCLTNLREFGAPIKGIKNQ
                     ATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIKS
                     LSLGLVIPDITFELAKANKDMALFSPYDIERVYGKPMSDISITEEYETLLANADIRKT
                     FISARKLFQTIAELHFESGYPYILFEDTVNAKNPHKKEGRIVMSNLCSEIAQVNTASQ
                     FSEDLTFTKVGHDVCCNLGSINIARAMDQAADFEKLIANSIRALDRVSRTSDLDSAPS
                     IKKGNAANHAVGLGAMNLHGFLATNHIYYDSQEAIDFTDCFFYAMAYYAFKASNHLAK
                     EKGTFEGFSESSYADGSYFYQYTEQNFEPKTQRVKNLLAEYGLTLPSQEDWRKLVQSI
                     KEIGLANAHLLAVAPTGSISYLSSCTPSLQPVVSPVEVRKEGALGRVYVPAYKIDADN
                     YVYYKKGAYEVGSEAIINIAAAAQKHIDQAISLTLFMTDQATTRDLNKAYIQAFKQKC
                     ASIYYVRVRQDILEGSESYDDMLDDFTSSDLEDCQSCMI"
     gene            3941..7108
                     /locus_tag="VBC74_001557"
     CDS             3941..7108
                     /locus_tag="VBC74_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEO6942495.1"
                     /translation="MTKKHLKTLALALTTVSVVTYSQEVYGLEREESVKQEQTQSASE
                     DDWFEEDNERKTNVSKENSTVDETVSDLFSDGNSNNSSSKTESVVSDPKQVPKAKPEV
                     TQEASNSSNDASKVEVPKQDTASKKETLETSTWEAKDFVTRGDTLVGFSKSGINKLSQ
                     TSHLVLPSHAADGTQLTQVASFAFTPDKKTAIAEYTSRLGENGKPSRLDIDQKEIIDE
                     GEIFNAYQLTKLTIPNGYKSIGQDAFVDNKNIAEVNLPESLETISDYAFAHMSLKQVK
                     LPDNLKVIGELAFFDNQIGGKLYLPRHLIKLAERAFKSNRIQTVEFLGSKLKVIGEAS
                     FQDNNLRNVMLPDGLEKIESEAFTGNPGDEHYNNQVVLRTRTGQNPHQLATENTYVNP
                     DKSLWRATPDMDYTKWLEEDFTYQKNSVTGFSNKGLQKVRRNKNLEIPKQHNGITITE
                     IGDNAFRNVDFQSKTLRKYDLEEIKLPSTIRKIGAFAFQSNNLKSFEASEDLEEIKEG
                     AFMNNRIGTLDLKDKLIKIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGALHLMFIG
                     NKVKTIGEMAFLSNKLESVNLSEQKQLKTIEVQAFSDNALSEVVLPPNLQTIREEAFK
                     RNHLKEVKGSSTLSQITFNAFDQNDGDKRFGKKVVVRTHNNSHMLADGERFIIDPGKL
                     SSTMVDLEKVLKIIEGLDYSTLRQTTQTQFREMTTAGKALLSKSNLRQGEKQKFLQEA
                     QFFLGRVDLDKAIAKAEKALVTKKATKNGHLLERSINKAVLAYNNSAIKKANVKRLEK
                     ELDLLTDLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSD
                     TIGEGQKDAYGNPILNVDEDNEGYHTLAVATLADYEGLYIKDILNSSLDKIKAIRQIP
                     LAKYHRLGIFQAIRNAAAEADRLLPKTPKGYLNEVPNYRKKQVEKNLKPVDYKTPIFN
                     KDLPNEKVDGDRAAKGHNINAETNNSVAVTPIRSEQQLHKSQSDVNLPQTSSKNNFIY
                     EILGYVSLCLLFLVTAGKKGKRARK"
     gene            7121..7510
                     /locus_tag="VBC74_001558"
     CDS             7121..7510
                     /locus_tag="VBC74_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEO6942496.1"
                     /translation="MITQEMKEIINSQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGTYYEAAKKWAEGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEIT"
BASE COUNT         2544 a         1204 c         1485 g         2291 t
ORIGIN      
        1 attatgatga aaacacaaga tatagtggtg atatatgttg aaagacacaa aactttgtgt
       61 tagtatactg tttaagcaga tccttaataa aggtaaccga caaaaaagtt taacgtgact
      121 taacaaaaca gtggttttga agcaagagtt agcttcttac cagaataaaa aaacatcaag
      181 aagaacattc caatagttgt gtgattttaa caaaaatcca gtcctcttgg gaggcttttt
      241 tggtgcttaa aactcgctta attttaatat ttaggagact agtttaatgc aatcatacta
      301 tgatcgttct caatcaccac ttgactatgc cttgtcagaa aaagcttttc ctatgcgttc
      361 tgttaattgg aataagctaa acgatgacaa agacttagag gtatggaaca gagtaactca
      421 aaatttctgg ttacctgaaa agatcccagt ttctaatgac cttaattctt ggagaacatt
      481 ggatgctgat tggcagcaat taattactcg gacttttact ggtctaacct tattagattc
      541 tgtgcaggct actgttggtg atattgctca gattaagcat tctcagacag atcatgaaca
      601 agttatttat gctaactttg cctttatggt agctattcat gcgcgatctt atggaacgat
      661 tttttcaacc ttatgcacta gccaacaaat tgaggaagca catgaatggg tagtggatac
      721 tgaaagtcta caagctcgat cacgtatact tattcctttt tatactggtg acgatccttt
      781 gaaatctaag gttgctgcag ccatgatgcc aggatttttg ttatatggtg gcttttacct
      841 tcctttttac ctttcagcca gaggaaaatt accaaatacg tctgatatta ttcgtttaat
      901 tttacgagat aaggtaattc ataactatta cagtggctat aagtatcaac agaaagttgc
      961 taaattatct gtagaaaagc aagccgaaat gaagacattt gtctttgacc tcctctatca
     1021 gttgattgat ttagaaaaag cctatcttta cgaactatat gatggttttg atttggctga
     1081 agatgcaatc agatttagca tttacaatgc tggtaaattt ttacaaaatt taggatatga
     1141 ttctccattt acagaagaag agactcgtat atctcctgag gtttttgcac aactttcggc
     1201 gcgtgcagat gaaaatcatg atttcttctc aggaaacggc tcttcatata ttatggggat
     1261 tacagaagaa acattggatg aagattggga gttttaaatg gatagtacag tgaaggtggt
     1321 ctatttttca tcgaaatcta acaataccca tcgttttgtt caaaagttgg cttgttctaa
     1381 ccagcgtatt ccatcagatg gtagtagtat attggttact gaggattata ttttgattgt
     1441 gcctacttac gctggaggag gagatgatac caaaggcgca gttccaaaac aagttgtaca
     1501 gtttttaaat gttcgacaaa atcgggaaca ttgccaaggg gttatttctt ctggaaatac
     1561 caattttggt gacacttatg ccattgctgg acctattatt gctcgaaaat taaatgttcc
     1621 tttgcttcat caatttgaat tactgggaac acaagaagat gttacaaggg taaaggagtt
     1681 actttgccaa ttcactagaa aggataaata agatgacaca atcagatgca tatctctcgt
     1741 tgaacgcgaa gacacgcttt agagatcgca caggtaatta tcattttact tcggataaag
     1801 aggctgttga acaatatatg atagaacatg ttgaacctaa tacgatggtg ttcacatcac
     1861 taattgaaaa gctagattat ttggtttcta ataactacta tgaatcggac cttctaaaac
     1921 aatataacct tgagtttatt tgccaaattt ttgagcatgc atacgctaag aaatttgctt
     1981 ttctaaattt tatgggggct ttaaaatttt ataatgctta tgctcttaaa acagaagaca
     2041 atcgttatta tttggaacac tatgaagatc gtgttgtaat gaatgctctg tttttagctg
     2101 ctggtgatga aaaagctgcc tatgacctag tagatgacat gctagcaaat cgctttcaac
     2161 cagcaacacc taccttttta aatgcaggga aaaaacgtcg tggtgaatat atctcttgct
     2221 atttgttgcg cattgaagac aatatggaat ctatttctcg tgctattagt acaagccttc
     2281 agttatcaaa acgaggtggt ggggtagcac tttgcttaac caacttacgg gaatttggtg
     2341 ctccaataaa aggtattaaa aatcaagcta ctggtattgt acctgtcatg aaattactag
     2401 aagattcgtt ttcttacgcc aatcaattag gacaaagaca aggagctggt gcagtttact
     2461 tacacgctca ccatcctgaa gtattaacgt ttttagatac taaacgtgag aatgccgatg
     2521 agaaaattcg aattaaatct ctttccctag gacttgttat tcctgatatt acttttgaat
     2581 tggctaaggc aaataaagat atggccttgt tttcaccgta tgacattgag cgagtatatg
     2641 gtaaaccaat gtctgatatc tctatcacag aagaatatga aactttatta gccaatgcgg
     2701 atattcgtaa aacctttatt agtgcacgaa aactatttca aacgatagca gagcttcatt
     2761 ttgaatctgg ttatccttat atcttatttg aagatactgt aaatgctaaa aaccctcaca
     2821 aaaaagaggg acgaattgtt atgtctaatt tatgttctga gattgcacaa gttaatacag
     2881 ctagtcaatt ttcagaagac ctgactttca ctaaggttgg acatgatgtt tgttgtaatt
     2941 taggttctat taatattgcg cgtgctatgg atcaagcggc ggattttgaa aaattaattg
     3001 ctaatagcat tagagccctt gatcgtgtat ctcgaacttc tgatttagat agtgctccat
     3061 ctattaaaaa aggtaatgca gctaatcatg ctgttggttt aggtgctatg aatttgcatg
     3121 gatttttggc gactaaccat atttattacg attctcaaga ggctattgat tttacagatt
     3181 gcttctttta cgctatggct tattatgcct ttaaagcttc taaccattta gcaaaagaaa
     3241 aaggaacctt tgagggcttc tcggaatcaa gttatgctga tggcagttat ttctatcaat
     3301 ataccgaaca aaactttgag ccaaaaacac agcgtgttaa aaatctacta gctgagtatg
     3361 gtctgactct accaagccaa gaggattgga gaaagcttgt gcaaagtatt aaagagatag
     3421 gattagctaa tgcgcattta ttagctgttg ctccgacagg gtcaatcagt tatctttctt
     3481 cttgtactcc gagccttcaa ccggttgtat cacctgtcga agtacgcaag gaaggagcac
     3541 tggggagggt ttatgtacct gcttataaga ttgatgcaga taattatgtc tactacaaaa
     3601 aaggagctta tgaagtggga tctgaggcga ttatcaatat tgcagctgcc gctcaaaaac
     3661 acattgatca agctatttcg ttaacgcttt tcatgacaga tcaagcaact acgcgagatt
     3721 taaataaagc ctatattcaa gcatttaaac aaaaatgtgc ctctatttat tatgtacgag
     3781 tgagacagga catcctagaa ggtagcgaga gttatgatga tatgctggat gatttcactt
     3841 catcggactt agaagactgt caatcctgca tgatttaact aaatatactc aatcagaaga
     3901 ttaagagaaa tatattggct tttagaaagg ctttattgta atgacaaaaa aacatcttaa
     3961 aacgcttgcc ttggcactta ctacagtatc agtagtgaca tacagccagg aggtatatgg
     4021 attagaaaga gaggaatcgg tcaaacaaga acaaacccag tcagcttcag aagatgattg
     4081 gttcgaagaa gataatgaga ggaaaacaaa tgtttctaaa gagaattcta ctgttgatga
     4141 aacagttagt gatttatttt ctgatggaaa tagtaataac tctagttcta aaaccgagtc
     4201 agtggtaagt gaccctaaac aagtccccaa agcaaaacca gaggttacac aagaagcaag
     4261 caattctagt aatgatgcta gcaaagtaga agtaccaaaa caggatacag cttcaaaaaa
     4321 ggaaactcta gaaacatcaa cttgggaggc aaaagatttc gtaactagag gggatacttt
     4381 agtaggtttt tcaaaatctg gaattaataa gttatctcaa acatcacact tggttttacc
     4441 aagtcatgca gcagatggaa ctcaattgac acaagtagct agctttgctt ttactccaga
     4501 taaaaagacg gccattgcag aatatacaag taggctagga gaaaatggga aaccgagtcg
     4561 tttagatatt gatcagaagg aaattattga tgagggagaa atatttaatg cttaccagtt
     4621 gactaagctt actattccaa atggttataa gtctattggt caagatgctt ttgtggacaa
     4681 taagaatatt gctgaggtta accttcctga gagtctcgag actatttcag actatgcttt
     4741 tgctcacatg tctttaaaac aagtaaagtt accagataac ctaaaggtca ttggagaatt
     4801 agcttttttt gataatcaga ttggtggtaa gctttacttg ccacgtcact tgataaaatt
     4861 agcagaacgc gctttcaaat ctaatcgtat tcaaacagtt gaatttttgg gaagtaagct
     4921 taaggttata ggagaagcaa gttttcaaga taataatctg aggaatgtta tgcttccgga
     4981 tggacttgaa aaaatagaat cagaagcttt tacaggaaat ccaggagatg aacattacaa
     5041 caatcaggtt gtattgcgca caaggacagg ccaaaatcca catcaacttg cgactgagaa
     5101 tacttacgtc aatccggaca aatcattgtg gcgtgcaaca cctgatatgg attataccaa
     5161 atggttagag gaagatttta cctatcaaaa aaatagtgtt acaggttttt caaataaagg
     5221 cttacaaaag gtaagacgta ataaaaactt agaaattcca aaacaacaca atggtattac
     5281 tattactgaa attggtgata acgcttttcg caatgttgat tttcaaagta aaactttacg
     5341 taaatatgat ttggaagaaa taaagctccc ctcaactatt cggaaaatag gtgcttttgc
     5401 ttttcaatct aataacttga aatcctttga agcaagtgaa gatttagaag agattaaaga
     5461 gggagccttt atgaataatc gtattggaac tctagacttg aaagacaaac ttatcaaaat
     5521 aggtgatgct gctttccata ttaatcatat ttatgccatt gttcttccag aatctgtaca
     5581 agaaatagga cgttcagctt ttcgacaaaa tggtgcgctt caccttatgt ttatcggaaa
     5641 taaggttaaa acaattggtg aaatggcttt tttatccaat aaactggaaa gtgtaaatct
     5701 ctctgagcaa aaacaattaa agacaattga ggtccaagct ttttcggata atgcccttag
     5761 tgaagtagtc ttaccgccaa atttacagac tattcgtgaa gaggctttca aaaggaatca
     5821 tttgaaagaa gtgaagggtt catctacatt atctcagatt acttttaatg cttttgatca
     5881 aaatgatggg gacaaacgct ttggtaagaa agtggttgtt aggacacata ataattctca
     5941 tatgttagca gatggtgagc gttttatcat tgatccaggt aagctatctt ctacaatggt
     6001 agaccttgaa aaggttttaa aaataatcga aggtttagat tactctacat tacgtcagac
     6061 tactcaaact cagtttagag aaatgactac tgcaggtaaa gcgttgttat caaaatctaa
     6121 cctccgacaa ggagaaaaac aaaaattcct tcaagaagca caatttttcc ttggtcgcgt
     6181 tgatttggat aaagccatag ctaaagctga gaaggcttta gtgaccaaga aggcaacaaa
     6241 gaatggtcat ttgcttgaga ggagtattaa caaagcggta ttagcttata ataatagtgc
     6301 tattaaaaaa gctaatgtta agcgcttgga aaaagagtta gacttgctga cagatttagt
     6361 cgagggaaaa ggaccattag cgcaagctac aatggtacaa ggagtttatt tattaaagac
     6421 gcctttacca ttgccagaat attatatcgg attgaacgtt tattttgaca agtctggaaa
     6481 attgatttat gcacttgata tgagtgatac tattggcgag ggacaaaaag atgcatatgg
     6541 taatcctata ttaaatgttg acgaggataa tgaaggttat cataccttgg cagttgccac
     6601 tttagctgat tatgaaggtc tttatattaa agatatttta aatagttccc ttgataagat
     6661 taaagcaata cgccagattc ctttggcaaa atatcataga ttaggaattt tccaagctat
     6721 ccgaaatgca gcggcagaag cagaccgatt gcttcctaag acacctaagg ggtacctaaa
     6781 tgaagtccca aattatcgta aaaaacaagt ggagaaaaat ttaaaaccag ttgattataa
     6841 aacgccgatt tttaataagg atttacctaa tgaaaaggta gacggtgata gagcggctaa
     6901 aggtcataat ataaatgcgg agactaataa ttctgtagct gtaacaccaa taaggtccga
     6961 gcagcaatta cataagtcac agtctgatgt aaatttacct caaacaagtt ctaaaaataa
     7021 ttttatatac gagattctag gatacgttag tttatgtttg cttttcctag taactgctgg
     7081 gaaaaaagga aaacgagcaa gaaaataagg agaaattaat atgattacac aagaaatgaa
     7141 agagattatt aatagtcagt tagcaatggt cgcgacggtt gatgctaaag gtcaacccaa
     7201 tattggccca aaacgttcta tgcgtctctg ggatgataaa acgtttattt ataatgaaaa
     7261 tacagatggt caaactcgta ttaacattga agataatggg aaaattgaaa ttgcctttgt
     7321 tgatcgtgaa cgtcttttag gttacaggtt tgtgggaact gctgaaattc aaacagaagg
     7381 tacttattac gaagcagcga aaaaatgggc agaaggacgc atgggggttc cgaaagcagt
     7441 tggtattatt catgtcgagc gtatttttaa tttacaatct ggtgctaatg caggtaaaga
     7501 aatcacttaa tataaaaaag cctc
//
LOCUS       DATROD010000082         7464 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.082, whole genome shotgun sequence.
ACCESSION   DATROD010000082 DATROD010000000
VERSION     DATROD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.082"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            114..1487
                     /locus_tag="VBC74_001559"
     CDS             114..1487
                     /locus_tag="VBC74_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6942497.1"
                     /translation="MLKKLFAPPQKRERLSAEEIKHKYPRYRVQVLISIFVGYMGYYF
                     VRNTTSILSGILNMSATEIGIITCASYIAYGLSKFISGLISDESNSKIFLPVGLFLTG
                     LVNVLIGVIPSVITSIWLFTIMYLINGWLQGMGYPPGARTLVYWYDNKERIKYATIWN
                     LSHNFGGAIAPILTGVGLALAGNDSLNQARAAYWFPGVVACLLAVLVYFLQEDTPESI
                     GLPPIEEYHKEQYTNVVDSSDILEEPEVLGMGEIIKKYILPNTKLMWASLYSIFVYIL
                     RYGIVSWTPKFLATSVQDGGKGITATAGMGGFSLFEIGGIIGMLTAGYLSAKVFKNSK
                     PLTNVAFLVVAILLLAAYWFIPAGPQYMALDFIILLGLGASIYGPVMMVGLYAMELVP
                     KAAAGAASGLTGTFSYVGGATIATLAIGIIIDHFGWGVAFIIFGISGFAAIVCTLLSR
                     DKSLEYW"
     gene            complement(1645..1951)
                     /locus_tag="VBC74_001560"
                     /pseudo
     CDS             complement(1645..1951)
                     /locus_tag="VBC74_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2114..3100
                     /locus_tag="VBC74_001561"
     CDS             2114..3100
                     /locus_tag="VBC74_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEO6942498.1"
                     /translation="MSEKIRVLLYYKYVSIENAEEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWIHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENEAKYVRGCSPECRAHERNRYVQENGLSRQEWAERLEAIGESLPQVANI"
     gene            complement(3137..4264)
                     /locus_tag="VBC74_001562"
     CDS             complement(3137..4264)
                     /locus_tag="VBC74_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEO6942499.1"
                     /translation="MKHYQTIIIGAGAAGIGFGSAMQRLGLTNFLIIEKGHIGESFLR
                     WPRTTQFITPSFTTNGFGFPDLNAVIPDTSPAFSFEKEHLSGVEYARYLQLVAAHYNL
                     PIQNETSVLSIDKRDSLFVIKTSKGDFSADYLIMATGEFQNPNTIDIKGADLGMHYGQ
                     VDNFHIKSDNPFIIIGGNESACDALTHLVYLGNQVELYTDTFGRKESNPDPSISLSPL
                     TKERLKHIQDHKKEYYSISEGKKAIEIKQIGKQYQVTFDDGSTAESFHKPILSTGFLN
                     TCHLIDGIALFEYDKNQLPIVTEDDESTIVNNCFLIGPSLRQRDTIFCYIYKFRQRFV
                     PVINHIAQREGIILDEDTLEFFKENQMYLDNLACCDVNCDC"
     gene            4368..5387
                     /locus_tag="VBC74_001563"
     CDS             4368..5387
                     /locus_tag="VBC74_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001932930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEO6942500.1"
                     /translation="MFLLLCILSMWLMIFGVAFYYFGSLHQSLTSRIIYQFVLTVLLT
                     TTAWFMGAYFLAFEGHFKTVFQFQEADGKQIVNCLFQLCFALYAVVMLIGSIIDRVQT
                     KRLLLAVVSWLFLVYTPLAYLIWNSEGVFAKMGVLDFSGGMVVHLSAGLSSYILAHVI
                     GKSEHQHNKVKNDSLFLGMILITFGWFGFNMGPVGEWNSQAIMILLNTIFAIIGGGLA
                     WTLAAKWNGEEEKTGSLLNGIIVGLVTSTAGVGYLLTWQLLAVTFFASLFTYFVTDYV
                     AKAFAIDDVVSSFGMNGIGGLLGSLGVGLFKLSHMPVQLLALATTILLSIIMTYIISK
                     AIFRK"
     gene            5712..6518
                     /locus_tag="VBC74_001564"
     CDS             5712..6518
                     /locus_tag="VBC74_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6942501.1"
                     /translation="MSKKIILGILSLLSVVTLVACGSSDKQLQDKVEKKGKLVLAVSP
                     DYAPFEFKALVNGKDKVVGADIELAQAIADELHVKLEVSPMSFDNVLSSLQTGKADMA
                     ISGLSYTKERAKVYDFSKPYYTTENAVLVRKSDLGKYTSIESLKGKKIAVQKGSIEEG
                     VSKKSFKNSHVVSLTAMGEAISELKSGKVEAVDLEKPVAEGYLAQNPDLVLAKFALKT
                     GEGDAKAVALPKDSGKMLKTVNKVIKRLEKEDKYKVYISDAAKLTGNQVE"
     gene            6601..7104
                     /locus_tag="VBC74_001565"
     CDS             6601..7104
                     /locus_tag="VBC74_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEO6942502.1"
                     /translation="MVQGSINIEKYKDLALQKQGEHRKFLASLKKKAPKNLDKIVQQI
                     HQEVFQEIDCTACANCCKTLGPLFTEADISRISKHFRMKLSAFEDLFLEVDEDGDKVF
                     KAMPCPFLGDDNLCSIYDVRPKACREFPHTDRKKIYQINNLTIKNTLTCPAAYLFVEK
                     LKSRIGK"
     gene            7208..7384
                     /locus_tag="VBC74_001566"
     CDS             7208..7384
                     /locus_tag="VBC74_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEO6942503.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
BASE COUNT         2365 a         1068 c         1493 g         2538 t
ORIGIN      
        1 ccacatttaa aaaaataaga taaaatgata aaaaataaat tattaattaa ttgacagtgt
       61 gaattcatag ttgtataatt aaacacatct aaaaatcgga aaggtaattt gctatgttga
      121 aaaaactatt tgcaccccct cagaaaagag aacgtttatc agctgaggaa attaagcata
      181 aatatcctcg ttatcgtgta caagtcctta tttctatttt tgtaggttat atgggatact
      241 attttgttcg taatacgact tcaattttat ctggtatcct taatatgtct gctactgaga
      301 ttggtattat tacctgtgct agctacattg cttatggtct ctctaaattt atttctggtt
      361 tgatttcaga tgaatcgaat tctaaaattt tccttccagt tggtttgttt cttacaggac
      421 tagttaatgt gttaatcggt gttattccat ctgtaattac tagtatctgg ctctttacta
      481 tcatgtatct tattaatggt tggttacaag ggatgggata ccctcctgga gcgcgtacat
      541 tagtctattg gtatgataat aaagaaagaa ttaaatatgc taccatttgg aatttatctc
      601 acaattttgg tggagcaata gcgccgattt taactggtgt aggtctagct cttgctggta
      661 atgattctct taatcaagcg cgtgctgctt attggtttcc aggggtggtg gcttgtcttt
      721 tggctgttct tgtttacttt ttacaagaag atactccaga atctatcgga cttccgccta
      781 tagaagagta tcataaagag caatatacta atgttgtgga ttcaagtgac attttagaag
      841 agcctgaagt tttgggtatg ggcgaaatta taaaaaaata tattctacca aatactaaac
      901 tgatgtgggc atccttgtat tcaatatttg tatatatttt acgttacggt attgtctcat
      961 ggactcctaa gtttttagcg acgagtgtgc aagatggtgg aaaaggtatt actgcaacag
     1021 ctggtatggg tggtttttca ctttttgaaa taggtggtat tattgggatg ctaacagcag
     1081 gatatctatc tgccaaagtg tttaaaaact ctaaaccttt aaccaatgta gccttcttag
     1141 ttgtagcaat cttattacta gctgcctatt ggtttattcc agcaggacct caatatatgg
     1201 cattagactt cattatttta ctaggtttgg gggcatcaat ctacggtcct gttatgatgg
     1261 ttggcctgta tgctatggag ttagttccaa aagcagcagc tggagcagct tcaggattga
     1321 ctggcacatt tagttatgtc ggaggagcaa cgatcgctac tctagctatt ggtattatta
     1381 ttgaccactt tggttgggga gtagcattta taatatttgg tatttctgga tttgcggcta
     1441 ttgtctgtac cttgctttca agagataaga gtctagagta ttggtgatat tatttggtaa
     1501 aagtatcatt taatatttag tgagataagt tatgaaaaaa ggcctcttga aagagacctt
     1561 ttttgtttta gataatctat ttgttgaatt aattagtgat atcgattgtt tttaatagat
     1621 agtttctacc tagttaaaat aaaactaccc cattaataat gattcaattt taatgtcata
     1681 ttcagggtag tgtatgctta aatgttcgat aatcacatga taaatttgtg tgatttttgc
     1741 tcttttttct gggaagtcat gaccaatata gtggaaggaa cattctatcg ttaaaaagag
     1801 ctcatggaat aaggtattga cttcttctaa aagttctaaa agttttttta tcttcagtaa
     1861 taaacgcagg tgcagtattg atttcatcag cccaagcatc tacaggtcta agtggataag
     1921 taccgtattc tagacataat tcataagtca taaaagtcct cctagtcttt tactatttta
     1981 acatatgtgt gttcttaaga agaataattt gtctttaaag caaagaatat gtcctaagcg
     2041 tattttttgc tatgttgtaa ggagtcgtta tataatgaag tatactaact tattttgcag
     2101 atgaggagaa aaaatgtcag aaaaaatcag agttttactc tattataaat atgtttcaat
     2161 tgaaaatgcg gaagagtacg ctgcgaaaca tctagaattt tgtaaatcta ttgggcttaa
     2221 aggtcgtatt ttgattgcgg atgaggggat taatggaact gtttctggag attatgaaac
     2281 aactcagaag tatatggatt ggatccacag cgatgagcgt tttgctgatc tttggttcaa
     2341 gattgacgag gaaaatcaac aagctttccg taaaatgttt gttcgttata agaaagagat
     2401 tgtgcacctt gggttagaag ataataactt tgatagcgat attaatcctt tggaaacaac
     2461 aggtgagtat ttaaatccta aacaatttaa agaagccttg cttgatgaag atactgttgt
     2521 tttagatacg cgtaatgatt atgagtatga cttaggtcac ttccgtggag caattcgtcc
     2581 tgatattcgc aatttccgtg agttaccaca atgggtacgt gacaacaaag ataagtttat
     2641 ggaaaaacgt gttgttgtgt actgtaccgg tggtgttcgt tgtgagaaat tctcgggttg
     2701 gatggttcga gaaggtttta aagatgttgg acaattacac ggtggtattg caacatatgg
     2761 aaaagatccg gaagttcaag gtgaactttg ggatggtgcg atgtatgttt ttgatgatcg
     2821 tatctcggtg ccaattaatc atgttaatcc aactgttatt agtaaagatt attttgatgg
     2881 aacaccttgt gaacgttatg tcaattgtgc aaatccgttt tgtaacaaac aaatttttgc
     2941 ttcagaagaa aacgaagcga agtatgttcg agggtgctcg cctgaatgtc gtgcacatga
     3001 acgtaatcgc tatgtgcaag aaaatggttt atcacgtcaa gaatgggcag aacgtttaga
     3061 agctattgga gaatccttac ctcaagtagc aaatatttaa tattaaaaaa tcgctagcaa
     3121 taaagctagc gattttttag caatcacaat tgacatcgca acaagctagg ttatcaaggt
     3181 acatttgatt ttctttaaag aattcaaggg tatcttcgtc taatataatc ccttctcgtt
     3241 gtgcgatatg attaataaca gggacaaagc gctgtctgaa tttataaata tagcagaaaa
     3301 tagtatctct ttgtcgtaag cttggaccaa ttaagaaaca gttattaacg atagtagatt
     3361 cgtcatcttc agtaactatt ggcaactgat ttttatcgta ttcaaataaa gcgattccgt
     3421 caatgagatg acaggtattt agaaaacctg tggaaagaat aggtttatgg aaagactccg
     3481 cagtactacc gtcatcaaaa gtaacttgat attgttttcc aatttgttta atctcaatgg
     3541 ctttcttgcc ttcagagata ctataatatt cttttttgtg gtcttgtata tgttttaaac
     3601 gttctttagt taggggggat aaagagatgc ttggatcggg attgctctct ttacgaccaa
     3661 acgtatctgt atataattcg acttgatttc ccaaatatac tagatgtgtt agtgcatcac
     3721 aagcactttc gtttccacca atgatgataa atggattgtc agatttgata tggaaattat
     3781 caacttgacc atagtgcatt cctagatctg cacctttaat atcaatggta tttggatttt
     3841 gaaattctcc tgttgccata ataagataat cagcagaaaa atctcccttt gaagttttaa
     3901 taacaaaaag gctatctcgt ttatcaatag atagaacaga tgtttcgttt tggataggga
     3961 gattataatg agcagcaact aattgtaagt agcgtgcata ttccactcca gaaagatgtt
     4021 ctttttcaaa tgagaaagca ggagaagtat ctggaataac agcgtttaga tctggaaaac
     4081 caaagccatt ggtagtgaag gaaggtgtga taaattgtgt tgttctgggc catcttaaaa
     4141 aagattctcc aatgtgccct ttttcaatga taagaaaatt tgtaagtcct aatcgttgca
     4201 tggcagaacc aaaaccgatt cctgcagcgc ctgcaccgat aattatggtt tgataatgtt
     4261 tcatgtcttc tccttgacaa aaataaatgt gtaagataaa atacttaacc agttaacaac
     4321 aatacagttt aacaaataaa agagtgtttg taaagaaagg actatttgtg tttttactat
     4381 tatgtatttt atcgatgtgg ctcatgattt ttggggttgc attctattac tttgggtcgc
     4441 tacatcaatc attgacttca aggattattt atcagtttgt acttactgtt ttgctaacga
     4501 caacagcttg gttcatgggt gcctattttt tagcatttga aggtcatttt aaaactgtct
     4561 ttcaattcca agaagcagat ggtaaacaaa ttgtaaattg tttatttcaa ctttgtttcg
     4621 ccctttacgc agtagtaatg cttattgggt caattattga tagagttcag acgaagcgac
     4681 ttttattagc tgttgtttct tggcttttct tggtgtatac gcccttagct tatttaattt
     4741 ggaattcaga gggtgttttt gcaaagatgg gagtacttga tttttcaggc ggaatggtcg
     4801 ttcatttatc tgcagggctt tcttcgtata ttttagctca tgttattggc aagagtgaac
     4861 atcagcacaa taaggttaaa aatgatagtc tctttttagg aatgatactt attacatttg
     4921 gttggtttgg atttaatatg ggtccagttg gtgagtggaa ttcacaagcc attatgatac
     4981 ttttaaatac tatatttgct attataggtg gtggtttggc gtggacattg gctgctaagt
     5041 ggaatggtga ggaagagaaa acaggaagct tattgaatgg cattattgtt ggtcttgtga
     5101 cgagtacagc aggagttggg tatctcttaa cttggcaatt attagctgta acattctttg
     5161 catccctctt cacctacttt gtaacggatt acgttgcgaa agcttttgcc attgatgatg
     5221 tcgtttcctc ctttggaatg aatggtattg ggggattgtt gggaagttta ggagttggtc
     5281 tctttaaatt atcacatatg ccagttcaac tgttagcatt agctaccaca attcttttgt
     5341 caattataat gacctatatc atatctaaag cgatttttag aaaataataa ataaggttga
     5401 gatgtacatc tcaaccttat ttattatttt agcaaggaga gtgactaaat cgaattatta
     5461 tcattttctt actagacagc aactgtacaa ggttctgaaa gttagttgat tattaatgat
     5521 agttagcatt ttttaagaat tggaatactt ttaaaaggtg attgttttaa ttagttatag
     5581 ttgttattaa aaatcgtcta acagcaagca tagttatcgg tgtaatttac tgaattttta
     5641 ttcaaaaaaa cttgacttat gtataaagat gatttatact tgttcctatc ataataaaag
     5701 gaagatagtt tatgtcaaaa aaaataatat taggaatttt atctctttta tctgtcgtta
     5761 ctttggtggc gtgtggttca tcagacaaac agctacaaga taaagttgag aaaaaaggga
     5821 agttagtttt agcggtgagt ccagattatg ctccctttga gtttaaagcg cttgttaatg
     5881 ggaaagataa agttgtcggc gcagatattg agttagctca agctattgct gatgagcttc
     5941 atgtcaaact agaggtatct cctatgagtt ttgacaatgt tttatcaagt ttacaaacag
     6001 gtaaggctga tatggctatt tctggattat catatactaa agagcgtgct aaagtctatg
     6061 atttttcaaa accttattac actacagaaa atgcggttct tgttaggaag tctgatttag
     6121 gaaaatatac ttcaatcgaa agccttaaag gtaaaaaaat agcagtacaa aaaggaagta
     6181 tcgaagaggg agtttctaag aaatcattca aaaattctca cgtcgtgtca ctgacagcga
     6241 tgggagaagc gattagtgag ttaaaatcag gcaaggtaga agctgttgat ttagaaaaac
     6301 ccgtagctga aggttacttg gcccagaatc cagaccttgt tcttgctaag tttgccctta
     6361 aaacaggaga aggggatgct aaggctgtag ctttacctaa agacagtgga aaaatgttga
     6421 agactgttaa caaagtgata aagagacttg aaaaagaaga taaatataaa gtttatattt
     6481 cagatgcggc taaattaact ggcaatcaag ttgaataata ttaaaaagcc tagtcatagg
     6541 cttttttgga tgttataata tggcttgact aagaataata gcataaaggc agaggaatat
     6601 atggttcagg gttcaattaa tattgaaaaa tataaagatt tggcgctcca aaaacaaggc
     6661 gagcatcgta aatttttagc aagtttaaaa aagaaagctc ctaaaaactt agataaaata
     6721 gtccaacaga tacatcaaga ggtttttcaa gaaattgatt gtacagcatg tgctaattgt
     6781 tgtaaaactt tgggtcctct atttacagaa gctgatattt ctaggatttc taaacatttc
     6841 cggatgaagt tatcggcttt tgaagattta tttttagaag tagatgaaga tggagataaa
     6901 gtatttaaag cgatgccttg tccgttttta ggcgatgata acttatgtag tatatacgat
     6961 gtcagaccta aagcttgtcg tgaatttcca catactgatc gtaagaaaat ctatcaaatt
     7021 aataacttaa cgataaaaaa tactcttact tgtccagcgg catacctttt tgtggaaaaa
     7081 ttgaaatcac gtatagggaa ataataaccc agagaaagta tcataaaaat attgacaatc
     7141 tgcttttatt tgggtatcct agtaagtgaa cgtgaaaatg ttacaatatc ttgaggaggt
     7201 gaaacatatg tcaaaaacag tagtacgtaa aaacgaatca cttgacgatg cacttcgtcg
     7261 tttcaaacgt tctgtgacta aagctggtac tcttcaagaa tcacgtaaac gtgaattcta
     7321 cgaaaaacct tctgtaaaac gtaaacgtaa atcagaagca gctcgcaaac gtaaaaaatt
     7381 ctaattagat agaatatgaa aaaacagttc agaattgagc tgttttttca tatattataa
     7441 ctataagtta ataaaaaaac tcgg
//
LOCUS       DATROD010000083         7360 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.083, whole genome shotgun sequence.
ACCESSION   DATROD010000083 DATROD010000000
VERSION     DATROD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.083"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            138..1382
                     /locus_tag="VBC74_001567"
     CDS             138..1382
                     /locus_tag="VBC74_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO6942504.1"
                     /translation="MKIVEGVSLHLIKNQQFKTNHLTFRFSGDFNNKTVARRSLVAQM
                     LVTANAKYPKVQEFREKLASLYGASLSTKISTKGLVHIVDIDIVFVKNTFTLEQENIV
                     EQIITFLEDMLFSPLISLEQYQTSIFDTEKKNLIQYLEADIEDNFYSSDLALKSLFYN
                     NKTLRLPKYGTASLVESENSFTAYQEFQKMLKEDQLDIFVVGDFDDYRMIQAFNRMAF
                     EPRHKVLAFDYTQTYENITRSQVEDKDVNQSIMQLAYHLPITYKDEDYFALIVFNGLF
                     GAFAHSLLFTEIREKQGLAYTIGSQFDSFTGLFTIYAGIDKENRERFLKLINKQFNNI
                     KMGRFSSTLLKQTKDILKMNYVLASDNPKVIVDHIYHEHYLDQFHTSALFIDKVDDVT
                     KSDIVSVATKLKLQAFYFLEGN"
     gene            1384..2667
                     /locus_tag="VBC74_001568"
     CDS             1384..2667
                     /locus_tag="VBC74_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEO6942505.1"
                     /translation="MTVNKITYQNLQEQVYKLTLESGLNVYLIPKPSFKETVGVLTAN
                     FGSLHTRYTRNGCVEHYPAGIAHFLEHKLFELDKGQDAATQFTKYGAESNAFTTFDKT
                     SFYFSTISHITNCLDILLDFVLTTNFTEESITKEKDIIKQEIEMYQDDPEYRLYQGVL
                     SNLYPNSPLAFDIAGDYQSISQITLTDLQENHKDFYQLSNMNLVLVGQFSPQEIITYL
                     QKNSHLTNYSQNIDRDSISLEPVIKNNSCHMTVTKPKLAIGYRKSSHMRHGSYLKEKI
                     GLQLFFAMLLGWTSTINQDWYESGQIDDSFDIEIEVHPDFECVIISLDTTEPIAFSTQ
                     LRLLLKNALQSSDLTESHLQNVKRELYGDFLRSLDSIENLAMQFVTYLYDGKTMYLDL
                     PSIVEELDLEDVITIGKDFLDNADTSDFVIFPKSS"
     gene            2790..3332
                     /gene="pgsA"
                     /locus_tag="VBC74_001569"
     CDS             2790..3332
                     /gene="pgsA"
                     /locus_tag="VBC74_001569"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEO6942506.1"
                     /translation="MVKKENIPNLLTVVRILMIPLFIVLTSVTTSTTWHIVAAIVFAI
                     ASLTDYLDGYLARKWQVVTNFGKFADPLADKMLVMSAFIMLVGLDLAPAWVSAIIICR
                     ELAVTGLRLLLVETGGTVLAAAMPGKIKTATQMFAVIFLLVHWMTLGNIMLYIALFFT
                     LYSGYDYFKGAGFLFKDTFK"
     gene            3332..4171
                     /locus_tag="VBC74_001570"
     CDS             3332..4171
                     /locus_tag="VBC74_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO6942507.1"
                     /translation="MTNIITVNNLFFKYDSNQTHYQLENVSFHVKQGEWLSIIGHNGS
                     GKSTTVRLIDGLLEAESGQIIIDGQELTEDNVWELRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIPLKDMKERVDQALDLVGMSEFKMREPARLSGGQKQRVAIAGAVAMRPQ
                     VIILDEATSMLDPEGRLELIRTIRAIRQKYNLTVISITHDLDEVALSDRVIVMKNGKV
                     ESTSTPKALFGRGNRLISLGLDVPFTSRLMAELAANGLDIGTEYLTEKELEEQLWELN
                     LKM"
     gene            4147..4989
                     /locus_tag="VBC74_001571"
     CDS             4147..4989
                     /locus_tag="VBC74_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEO6942508.1"
                     /translation="MGIEFKNVSYTYQAGTPFEGRALFDVNLKIEDVSYTAFIGHTGS
                     GKSTIMQLLNGLHIPTKGEVIVDDFSIKAGDKNKEIKFIRQKVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGISQIEAERLAEEKLRLVGISEDLFDKNPFELSGGQMRRVAIAGIL
                     AMEPKVLVLDEPTAGLDPKGRKELMTLFKNLHKKGMTIVLVTHLMDDVADYADYVYVL
                     EAGKVTLSGQPKQIFQEVELLESKQLGVPKITKFAQRLSHKGLNLPSLPITINEFVEA
                     IKHG"
     gene            4982..5776
                     /locus_tag="VBC74_001572"
     CDS             4982..5776
                     /locus_tag="VBC74_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO6942509.1"
                     /translation="MDKLILGRYIPGNSLIHKLDPRSKLLAMLLFIIIVFWANNVVTN
                     VIVFIFTLVIVGLSQIKFSYFFNGIKPMVGIILFTTLFQMLFAQGGQVIFSFWIFSIT
                     SLGLQQAALIFMRFVLIIFFSTLLTLTTTPLSLADAVESLLKPLEVLRVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIHKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGANRSKYRLLKWTVRDTFSILLMLLLGLSLFLLKN"
     gene            5992..6531
                     /locus_tag="VBC74_001573"
                     /pseudo
     CDS             5992..6531
                     /locus_tag="VBC74_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            6637..7341
                     /locus_tag="VBC74_001574"
     CDS             6637..7341
                     /locus_tag="VBC74_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEO6942510.1"
                     /translation="MNKRRKLSKLNVKKQHLAYGAITLVALFSCILAVTVIFKSSQVT
                     TESLSKADKVRVAKKSKMTKATSKSKVEDVKQAPKPSQASNEAPKSSSQSTEANSQQQ
                     VTASEEAAVEQAVVTENTPATSQAQQTYAVTETTYKPAQHQTSGQVLSNGNTAGAVGS
                     AAAAQMAAATGVPQSTWEHIIARESNGNPNVANASGASGLFQTMPGWGSTATVQDQVN
                     SAIKAYRAQGLSAWGY"
BASE COUNT         2451 a         1138 c         1368 g         2403 t
ORIGIN      
        1 aatattggtt gacgactttg tcaattttga agaagattga aatttagaat taatcatatc
       61 ttggatgttt aatctttttt aacgccttta aaaatgatat aatcttagct aacaatacag
      121 aacacaaagg atattgaatg aaaatagttg aaggcgtttc tctacattta attaaaaacc
      181 aacaatttaa gactaatcat cttacttttc gattttcagg tgattttaat aataaaacag
      241 tagcacgtag atcgctcgtt gctcaaatgt tagttactgc aaatgctaag taccctaagg
      301 tacaagaatt tcgagaaaag ttagcttcct tatatggtgc tagtttatca actaagattt
      361 ctactaaggg tcttgttcac attgttgata ttgatattgt atttgttaaa aataccttta
      421 ctttagaaca agaaaatatc gttgaacaaa ttattacatt cttggaggat atgctgtttt
      481 ctcctttaat ttctttagaa cagtatcaaa catctatttt tgatactgag aagaaaaacc
      541 ttattcaata tttagaggcc gatattgaag ataattttta tagcagtgac ttagcactaa
      601 aatctttatt ctacaataat aagacacttc gtttacctaa gtatggtaca gcatctttag
      661 tggaatcaga aaattcattt acagcttatc aagaatttca gaagatgctt aaggaagatc
      721 aattagatat ttttgtcgta ggagattttg atgactatcg aatgattcag gcatttaatc
      781 gtatggcatt tgagccacgt cacaaagtat tagcttttga ttatacgcag acttatgaaa
      841 atatcacgag aagtcaagta gaagataagg acgttaatca atctattatg caattggctt
      901 atcatcttcc aataacttat aaagatgaag attattttgc tttaatagta tttaacggtt
      961 tatttggtgc ttttgcccat tccttgttgt ttacagaaat tcgagaaaag caaggtttag
     1021 cctatactat aggtagtcaa tttgacagct ttacaggtct ctttacgata tatgcaggta
     1081 ttgataaaga gaatcgtgag cgttttttga agcttattaa taagcaattc aataatatta
     1141 agatgggcag gttctcttct acattattga aacaaacaaa agatatattg aaaatgaact
     1201 atgttttagc ttctgataat cctaaggtca tagttgacca catttatcat gaacattatt
     1261 tagatcagtt tcatacttca gcactattca ttgacaaagt ggacgatgta acaaaatcag
     1321 atattgtcag cgtagcaaca aaattaaaat tacaagcttt ttattttttg gaaggaaact
     1381 aggatgacag ttaataaaat aacttatcag aaccttcagg agcaagtata taagttaaca
     1441 ttagagagcg ggctaaatgt atatcttatt ccaaaaccat catttaagga aactgttgga
     1501 gtattgacag caaattttgg atcactccat acaagatata cgaggaatgg ttgtgtagaa
     1561 cattatccgg caggaattgc tcacttctta gaacataaat tatttgaact ggataaagga
     1621 caagacgctg caactcaatt tactaaatat ggcgccgaga gtaatgcctt tacaactttt
     1681 gataaaacta gtttttattt ttcaactata agtcacatta caaactgctt agatatacta
     1741 cttgactttg tattgacaac aaactttaca gaagagtcaa ttactaaaga aaaagacatt
     1801 atcaaacaag aaattgaaat gtatcaagat gacccagaat ataggcttta tcaaggagtg
     1861 ttatcgaacc tttaccccaa ctctccatta gcatttgata tcgctggaga ttaccaatca
     1921 atctctcaaa taaccttaac cgatttacaa gaaaatcaca aagactttta tcagttgtct
     1981 aatatgaatt tagttcttgt tggacaattt agtccacaag aaattataac ttaccttcaa
     2041 aaaaattctc atttaacaaa ttactcacag aatattgacc gagattctat tagtcttgaa
     2101 cctgttataa aaaacaactc ttgccatatg acagtgacaa aacctaaatt agctattggt
     2161 tatcgaaaat caagtcatat gagacatggt tcttacctaa aagagaaaat cggattacag
     2221 ctattttttg caatgctttt aggatggact tctacaatta atcaagactg gtacgaatct
     2281 ggtcaaattg atgactcttt tgatatagaa attgaagttc atccagattt tgagtgcgtt
     2341 attatttctt tagatacgac agagccaatt gctttttcaa ctcagttgag gttattgcta
     2401 aaaaatgctt tgcaatcatc tgatcttacc gaaagccact tacaaaacgt taagagagag
     2461 ctctatggcg attttttaag gagcttagat tctattgaaa atctcgcaat gcaattcgtc
     2521 acctatttat atgatggaaa aacgatgtat ttagatttgc catctatcgt tgaagaatta
     2581 gacttggaag atgtcattac gattggtaag gatttcttag ataatgctga cacatctgac
     2641 ttcgttattt ttccaaaatc ttcgtaaata atctgaaatt ttgatataat aaggaagctt
     2701 agacatctat aaagttggta tgtcaaaagt cttttactag ttttgaaata acataatata
     2761 ggaatactat gtttccaaaa ggagaattta tggtgaagaa agaaaatatt ccgaatctat
     2821 taactgtagt cagaatttta atgattccgt tatttatagt attaacttca gtaacaacaa
     2881 gtacaacttg gcatattgtt gcagctattg tatttgctat tgcaagcttg accgattacc
     2941 tagacggtta tctagcacgt aaatggcaag tagtaacaaa ttttgggaaa tttgcagatc
     3001 cattagctga taaaatgcta gttatgagtg cttttatcat gttagttgga ctagacttag
     3061 caccggcttg ggtttcagct attatcattt gtagagagct agcagttaca ggtttgcgtt
     3121 tacttttagt tgaaacaggt ggaacagtac ttgctgcagc tatgccaggg aaaataaaga
     3181 cggctactca aatgtttgca gttatttttt tgttagtaca ttggatgaca cttggtaaca
     3241 tcatgttata cattgcttta ttctttacct tgtattcagg ctacgactat tttaaaggtg
     3301 caggcttctt atttaaggat acttttaaat aatgacaaat attattactg ttaataatct
     3361 cttttttaaa tatgatagta atcaaacaca ttatcaattg gagaatgtat cgtttcatgt
     3421 gaaacaagga gaatggctat ctattatcgg tcataatggt tctgggaaat ccactactgt
     3481 tcgtttaata gatgggcttt tagaggcgga gtcaggccag attatcattg atggacaaga
     3541 gttaaccgaa gataatgtct gggagttacg tcacaagatt ggcatggtat ttcaaaatcc
     3601 tgataaccaa tttgtagggg caactgttga agatgacgtc gcctttggtt tggaaaataa
     3661 aggtattcct ttgaaagaca tgaaagaaag agtggaccaa gcacttgatt tggtaggcat
     3721 gtctgaattt aaaatgaggg agcctgcacg tttatctggg ggacaaaagc agcgtgtagc
     3781 cattgcagga gctgtagcta tgcgtccaca agttatcatc ttggatgagg cgactagtat
     3841 gcttgatcct gagggacgat tggaactgat aagaacgata cgagctattc gtcaaaaata
     3901 taatcttact gttatttcaa ttacacacga cttagatgaa gttgcattga gtgatcgtgt
     3961 catcgttatg aaaaatggta aagttgagtc gacatccact ccaaaagcgt tatttggtcg
     4021 tggaaatcgt ttgattagtt taggtttaga tgttcccttt acaagtaggt taatggcaga
     4081 actggctgct aatggtcttg atatagggac agagtatctt acagagaagg aattagaaga
     4141 acaattatgg gaattgaatt taaaaatgta agttatacct atcaagccgg cactcctttt
     4201 gaagggcgtg ccctttttga cgtcaatctg aaaattgaag atgtttccta taccgcgttc
     4261 attgggcaca caggttctgg aaaatcaact attatgcaac ttttgaatgg tttacatatt
     4321 cctacaaaag gtgaggtaat tgtcgatgat ttttctatta aagcagggga caagaacaaa
     4381 gaaatcaaat ttataaggca aaaagttggt ttagtttttc aatttccaga aagtcagctt
     4441 tttgaagaga cagttttaaa ggatgttgct tttggaccac aaaattttgg tatttctcag
     4501 attgaagctg aaaggctggc tgaagaaaaa ttaaggttag ttggtatcag tgaggattta
     4561 ttcgataaaa atccatttga actttctgga gggcagatga ggcgggttgc tatagctggt
     4621 attttagcga tggaacccaa agtactagta ctggatgagc caacagctgg acttgatcct
     4681 aagggaagaa aagaattaat gactcttttt aaaaatcttc ataaaaaagg aatgactatc
     4741 gtcttagtga ctcacttaat ggacgatgta gcggattatg ctgactatgt gtatgtttta
     4801 gaagcaggga aagtaacctt atcaggacaa ccaaaacaga tttttcaaga agtagaactt
     4861 ttagaaagta aacaattagg agttcccaaa atcaccaagt ttgctcaaag actatctcat
     4921 aagggattaa atttacctag tttaccaatt actattaacg aatttgtgga ggctattaag
     4981 catggataaa ttgattttgg gacgttacat accaggtaat tctctcattc ataaattaga
     5041 tcctcgaagt aagttactag ccatgttgct ttttatcatt attgtttttt gggcaaataa
     5101 tgtagtgaca aatgtgatcg tattcatttt tacgttggtt attgtgggtt tatctcagat
     5161 aaaattttcc tattttttca atggtattaa accgatggtt ggtattattt tatttacaac
     5221 cctgttccag atgctgtttg cacaaggcgg gcaggttatc ttctcgtttt ggatttttag
     5281 catcactagc cttgggttac aacaagcagc acttattttt atgagatttg ttttaattat
     5341 cttcttttcg acattactta ccttaactac tactccgtta agtttagcag atgctgttga
     5401 atctttgtta aaaccattgg aagtactgag agtaccagca cacgagattg gtttgatgtt
     5461 atcccttagc ttacgtttcg ttcctacttt aatggatgat acaacacgta tcatgaatgc
     5521 tcaaagagct cgtggagttg attttggaga gggaaaccta attcataagg ttaagtccat
     5581 tatccctatt ttgattccat tatttgcttc tagttttaaa agagcagatg cgttggcaat
     5641 tgctatggaa gcaagaggtt atcaaggtgg agccaatcgt agtaagtacc gtttattgaa
     5701 gtggactgtt cgggatactt ttagtatatt actgatgtta ttattgggtt tgagtttgtt
     5761 tctattaaaa aattaaaagg ttaaggtcag tttatggtct tagccttttc atttatataa
     5821 tgtgaaaagc tcaacaactt tacttaaaga aaactaaaaa ttgttttttg gaggcctttt
     5881 ctttattaat ttacaattta tttgtaataa atttcatcta aggcaaaaca attagtaata
     5941 tttacaaggt atgatagtac gcgtatcaat taatgaaaga gagcatatat tatgtcaata
     6001 acctcggtta aaaaatcaaa accatttaaa ttaggagtgg caggtctttt agtgggtgct
     6061 tcattagctt taccactttc agtaagcgca gcatcttata ccgtgaaatc aggtgatacc
     6121 ttatcagcta ttgctaaaaa tcataaaact acggtacaat agttagtgtc tctcaatagt
     6181 atcagtaacg ctgatgtcat cagtataggt gatgttttaa aattggataa ttctacagct
     6241 agtcaagcag aagcaaaatc tcaaccaaca attgaaaatt caatgaattc ttcatcaaat
     6301 ttgagttcaa gtgattcagc tgcaaaagaa gaaatagctc gtcgtgaatc aaatggtagt
     6361 tatactgcac agaatggaca atattatgga agatatcaac tgtctcaatc ttacctaaat
     6421 ggcgacttat ctcctgaaaa tcaagaaaaa gtagcggaca attatgtggc ttctcgttac
     6481 ggatcttggt cggcagcgct atcattttgg aatagtaacg gttggtatta ataaaagttg
     6541 ataaaacaaa gagatttact taaaattaaa tctctttgta atatttttaa catctattca
     6601 attgcaaggt aacaaataaa gtataatata ggtaacatga ataaaagaag aaaattatca
     6661 aaattgaatg taaaaaaaca acatttagct tatggagcta tcactttagt agcccttttt
     6721 tcatgtattt tggctgtaac ggtcatcttt aaaagttcac aagttactac tgaatctttg
     6781 tcaaaagcag ataaagttcg cgtagccaaa aaatcaaaaa tgactaaggc gacatctaaa
     6841 tcaaaagtag aagatgtaaa acaggctcca aaaccttctc aggcatctaa tgaagcccca
     6901 aaatcaagtt ctcaatctac agaagctaat tctcagcaac aagttactgc gagtgaagag
     6961 gcggctgtag aacaagcagt tgtaacagaa aatacccctg ctaccagtca ggcacaacaa
     7021 acttatgctg ttactgagac aacttacaaa cctgctcaac accagacaag tggccaagta
     7081 ttgagcaatg gaaatactgc aggggcggtc ggatctgctg ctgcagcaca aatggctgct
     7141 gcaacaggag tccctcagtc tacttgggaa catattattg cccgtgaatc aaatggtaat
     7201 cctaatgttg ctaatgcctc aggagcttca ggacttttcc aaacgatgcc aggttggggt
     7261 tcaacagcta cagttcagga tcaagttaat tcagctatta aagcttatcg tgctcaaggt
     7321 ttatcagctt ggggttacta gtgaatagct ataaattaac
//
LOCUS       DATROD010000084         7311 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.084, whole genome shotgun sequence.
ACCESSION   DATROD010000084 DATROD010000000
VERSION     DATROD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7311
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.084"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..146)
                     /locus_tag="VBC74_001575"
     CDS             complement(<1..146)
                     /locus_tag="VBC74_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6942511.1"
                     /translation="MTKHKHLTLSDRNDIQSGLDRGETFKAIGLNLLKHPTTIAKEVK
                     RNKQL"
     gene            413..1747
                     /locus_tag="VBC74_001576"
     CDS             413..1747
                     /locus_tag="VBC74_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEO6942512.1"
                     /translation="MEDPGSQSLLLQFVILLILTLFNAFFSASEMALVSLNRSKVEQK
                     AEEGDKRYRRLLDVLENPNNFLSTIQVGITFISLLQGASLSASLGHVISGWLGNSATA
                     RTAGSIIALIFLTYVSIVLGELYPKRIAMNLKDRLAIVSAPIIIFLGKIVSPFVWLLS
                     ASTNLLSRITPMTFDDADEKMTRDEIEYMLTNSEETLDAEEIEMLQGIFSLDEMMARE
                     VMVPRTDAFMIDINNDAQSNIEGILSQNFSRVPVYDDDKDRVVGVLHTKRLLEAGFKT
                     GFDTIDLRKILQEPLFVPETIFVDDLLKALRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDTAEQFVREIDENIYIVLGTMTLNEFNDYFETELESDDVDTIAGYYLT
                     GVGSIPNQEEKVAYEVDSKDKRITLINDKVKDGRITKLKVLLSDIEQNIEKD"
     gene            1815..2603
                     /gene="pflA"
                     /locus_tag="VBC74_001577"
     CDS             1815..2603
                     /gene="pflA"
                     /locus_tag="VBC74_001577"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEO6942513.1"
                     /translation="MAEIDYKKVTGMIHSTESFGSVDGPGIRFIIFMQGCKMRCQYCH
                     NPDTWEMETNNSKERTVEDVLKEALRYKHFWGKDGGITVSGGEAMLRIDFITALFIEA
                     KKLGIHTTLDTCGFAYRATPEYHAILEKLLDVTDLVLLDLKEIDPEQHKIVTRQSNKN
                     ILQFARYLSDRGTPVWIRHVLVPGLTDIDDHLKRLGEFVQTLDNVDKFEVLPYHTMGE
                     FKWRELGIPYPLAGVKPPTPERVKNAKDIMKTESYTEYLKRIQN"
     gene            2720..3655
                     /locus_tag="VBC74_001578"
     CDS             2720..3655
                     /locus_tag="VBC74_001578"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEO6942514.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAWGLDTEAVALGTPN
                     EETAYVLDYFGVQAPRVVESAKAEGVETVILTDHNEFQQSISDIKDVTVYGVVDHHRV
                     ANFETANPLYMRLEPVGSASSIVYRMFKENGVSVPKELAGLLLSGLISDTLLLKSPTT
                     HASDIPVAKELAELAGVNLEEYGLEMLKAGTNLSSKTAAELIDIDAKTFELNGEAVRV
                     AQVNTVDINDILARQEEIEVAIQEAIVTEGYSDFVLMITDIVNSNSEILALGSNMAKV
                     EAAFEFTLENNHAFLAGAVSRKKQVVPQLTESYNA"
     gene            3715..4368
                     /locus_tag="VBC74_001579"
     CDS             3715..4368
                     /locus_tag="VBC74_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEO6942515.1"
                     /translation="MILTFNPGKLERQEFFKELINYLWIHDDVTLRQIKSHFTDYSKI
                     DRLLEEYINHGYILRQNKRYSLNLPFLSSLDGLALDDLVFIDSASQIYQLLQKRKFVT
                     NLDNQTNHLVFVEETDFERNTLTLSNYFYKLTNCYPLSREQKKLYQLLGDVNSEYALK
                     YMSSFILKFLRKDSVKQKRTDIFIQALELLGYISLNQDTTYRLNAKLDVEALKIYLT"
     gene            complement(4398..5399)
                     /locus_tag="VBC74_001580"
     CDS             complement(4398..5399)
                     /locus_tag="VBC74_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO6942516.1"
                     /translation="MIQKNKAPFVLISSVIILLLLILVSISLGYANTSVIDVLKLISG
                     KSDDAFLFIITNIRLPRIIVCIFGGASLGIAGLLLQTLTKNPLADSGILGINAGAGLV
                     IALTIGTFNVSNPTILYFLPLFAMFGGLVTIFLVYLMSYRRNHNISPTRLIITGIGIS
                     TMISGVMISIISQSNNQKMDMIVEWLSGKITISSWTTIITFIPILILLWGLAYSRSRH
                     LNIMNLNEQTALALGLHLKKERIYTLMLASSLAAISVVLIGNITFIGLLAGHLSRRLL
                     GNNHKIILPSCLLIGAIILLVSDTIGRLLLVGTGIPTGLVVSIIGAPYFLWLMTKVD"
     gene            complement(5396..6421)
                     /locus_tag="VBC74_001581"
     CDS             complement(5396..6421)
                     /locus_tag="VBC74_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEO6942517.1"
                     /translation="MFKASKKLVQKNKSNHFWLVFFITLILFLIGCYASLRFGAINFK
                     TSDLITVLKNPLKNSNAQDVIFDIRLPRIIAAILVGAAMSQAGAIMQGVTRNAIADPG
                     LLGINAGAGLALVVAYAFLGSMHYSTILIVCLLGSVISCLLVFTLSYTKQKGYHQLRL
                     ILAGAMISTLFTSVGQVVTLYFKLNRTVIGWQAGGLSQINWKMLIIIAPIIILGLLIS
                     QLLAHQLTILSLNESVAKALGQKTQLMTAFLLLIVLFLSASSVALIGTVSFIGLIIPH
                     FIKLFIPKDYRLLLPLIGFSGATFMIWVDLSSRIINPPSETPISSIISIVGLPCFLWL
                     IRKGKNL"
     gene            complement(6437..>7311)
                     /locus_tag="VBC74_001582"
     CDS             complement(6437..>7311)
                     /locus_tag="VBC74_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6942518.1"
                     /translation="QQTKEESTKTTISKMPKIEGFTYYGKIPENPKKVINFTYSYTGY
                     LLKLGVNVSSYSLDLEKDSPVFGKQLKEAKKLTADDTEAIAAQKPDLIMVFDQDPNIN
                     TLKKIAPTLVIKYGAQNYLDMMPALGKVFGKEKEANQWVSQWKTKTLAAKKDLHHILK
                     PNTTFTIMDFYDKNIYLYGNNFGRGGELIYDSLGYAAPEKVKKDVFKKGWFTVSQEAI
                     GDYVGDYALVNINKTTKKAASSLKESDVWKNLPAVKKGHIIESNYDVFYFSDPLSLEA
                     QLKSFTKAIKENTN"
BASE COUNT         2418 a         1159 c         1371 g         2363 t
ORIGIN      
        1 agttgcttat ttcgtttgac ttcttttgcg atagtagtag ggtgtttcag tagattaagc
       61 ccgatggctt taaaggtttc tcctctatcc aaaccagatt ggatgtcatt acggtctgag
      121 agagttaggt gtttgtgttt tgtcatgatg gataaactca tttctaaccc aaacgtctca
      181 gacttaattc caccataaaa atccttttca cactaacttc cggttttgga gggcagacgg
      241 aacttacttt gagaaattac taattgctat aaaatgccat aaacaatatt atcgactatg
      301 cgctcagtgc tattatatga tataataata gcattatcta atagaagaac agttggtaga
      361 gttaatcgct atcagctaaa ttcaaataat ttttatttag gagtaatttt ccatggaaga
      421 ccctggcagt cagagtttgt tactacaatt tgttatttta ttaatattaa ccctgtttaa
      481 cgccttcttt tcagcatctg aaatggcttt agtatcgcta aaccgctcta aagtagaaca
      541 gaaagctgaa gaaggggaca aaaggtatcg acgtcttttg gacgttcttg aaaatcccaa
      601 taatttttta tcaacaattc aagttggtat tacttttatt agccttttgc aaggggctag
      661 tttatctgcc tcactaggac atgtgatttc aggatggcta ggaaattcag caacagcccg
      721 tacagcaggg agtattattg cgctaatctt tttaacatat gtctctattg ttctaggaga
      781 attatatcct aagcgtattg ctatgaattt gaaagatcgt ctagcgattg tttcagctcc
      841 cattattatc tttctgggga aaatagtaag cccctttgta tggttgctct cagcttcaac
      901 taacttattg agtagaatca ctccgatgac ttttgatgat gctgacgaga agatgacacg
      961 tgatgaaatt gaatatatgt tgacgaatag tgaagaaact ttggatgctg aagaaattga
     1021 gatgctgcaa gggattttct cgctagatga aatgatggcg cgtgaagtta tggttccgcg
     1081 tactgatgct ttcatgattg acatcaacaa tgatgcacaa tcaaatattg aaggaatttt
     1141 atcacaaaac ttttctcgtg tccctgttta tgatgacgat aaagatagag ttgttggtgt
     1201 tttacatacc aagcgcttat tggaagcggg ctttaaaact ggttttgata ctatcgactt
     1261 gcgaaaaata cttcaggaac ctctttttgt tccagaaacg atttttgtag atgatctttt
     1321 aaaagcatta cgcaacactc aaaatcaaat ggccatttta ctagatgaat atggtggcgt
     1381 tgctggttta gtaactctag aggatttatt ggaagaaatt gtcggcgaaa ttgatgatga
     1441 aacagataca gctgaacaat ttgtcaggga aatcgatgag aatatctata ttgttctagg
     1501 aactatgacg cttaatgaat ttaatgatta ttttgagaca gagttagaaa gtgatgatgt
     1561 tgatacaatt gcgggttatt atttaactgg tgtgggtagt attccaaacc aagaggaaaa
     1621 agtagcttac gaagtagaca gcaaagataa acgcatcact ttgattaatg ataaagttaa
     1681 agatggtcgt ataacaaaat tgaaagtgct attgtctgat atagaacaga atattgaaaa
     1741 agactaagat tacttagtct ttttttcatg aaaatgatat aataaagtca taaaaacggt
     1801 tacaaggtga tgaaatggca gaaatagact ataaaaaagt aacaggaatg atccattcta
     1861 cagaaagttt cggctctgtg gatgggccag gtattcgttt tatcattttt atgcaaggat
     1921 gtaaaatgcg ttgtcaatac tgtcataatc ctgatacttg ggaaatggaa actaacaatt
     1981 caaaggaacg caccgttgag gatgttctaa aagaagctct tcgttataaa catttttggg
     2041 gaaaagatgg aggtatcaca gtttcaggtg gtgaagcaat gctgcgaatt gactttatta
     2101 cagctctctt tattgaggct aagaaactag gtattcatac gacgctagat acttgtggtt
     2161 ttgcttatcg agcaacacca gaataccatg ctatccttga aaaattgtta gatgttactg
     2221 atttggtact ccttgactta aaagaaattg atccagaaca acataaaatt gtaactcgtc
     2281 agtctaacaa aaatatttta cagtttgcta gatatttatc tgatagaggt actccggtat
     2341 ggattcgtca tgttttagtt cctggattga ctgatattga tgaccattta aaacgtttgg
     2401 gtgaatttgt ccaaacactt gataatgtcg ataaatttga agtattgcct tatcatacta
     2461 tgggagagtt caaatggcgt gaattaggaa ttccgtatcc attagctggt gtgaaaccac
     2521 caactccaga acgtgttaaa aacgctaaag atatcatgaa aacagaatcc tatacagaat
     2581 atctaaaaag aattcaaaat tagttttaag ttgaatttta ttcaactttt ttagttgcaa
     2641 agggcttcct agtaagattt tttgctaagt tattcaattt ttgctacaat aaggtgaata
     2701 taaaagaaga ggtaaaaaca tgtctaaaat tttagtcttt ggtcaccaaa atccagattc
     2761 agatgctatt ggatcatcag ttgcttttgc atatctagct aaagaagctt gggggcttga
     2821 cacagaggcc gttgctttag gaactccaaa tgaagaaaca gcatatgttt tagattattt
     2881 tggtgttcaa gcacctcgag tagtagaatc tgcaaaagca gagggtgtag agacagtcat
     2941 tttaactgac cataacgaat tccaacaatc tatttctgat attaaagatg tgacagttta
     3001 tggtgttgtt gatcatcatc gtgttgctaa ctttgagact gccaatccgt tatatatgcg
     3061 tttggaacca gttggctcag catcatcgat tgtttaccgt atgtttaagg aaaatggagt
     3121 atcagtaccc aaagagttgg ctggtttatt attatcaggt ttgatttcag atactcttct
     3181 tttgaaatca ccaacaactc atgcttcaga tattccagtt gcaaaagaac tagcagagct
     3241 tgcaggtgta aacttagaag aatatggttt agaaatgctt aaagcaggta ctaacctctc
     3301 aagtaagact gcagcggagt taattgatat tgatgctaaa acatttgaat taaatggaga
     3361 agctgttcgt gttgcacaag ttaatactgt ggacattaac gatattttag cacgtcaaga
     3421 agagattgaa gttgctattc aagaagcaat tgtcacagaa ggttattcgg attttgtatt
     3481 gatgattact gacattgtta attcaaattc agagatttta gctctcggtt caaatatggc
     3541 taaagttgaa gcagcttttg aatttacact tgaaaataac cacgcttttc ttgctggggc
     3601 agtatcacgt aaaaaacaag ttgttcctca attgacagaa agctacaatg cttaattaat
     3661 catgtaaaac gggctgcgtc ccgttttttt agtcatacat ttaggagcgt ttttatgata
     3721 ttaacgttta atccaggaaa gttagaaaga caagagtttt ttaaggagct tattaactat
     3781 ctttggatac atgatgatgt aacactaaga caaataaagt cccactttac agactattct
     3841 aaaattgacc gtttgttaga agaatatatt aatcatggat atatccttcg ccaaaataaa
     3901 cgctacagcc ttaatcttcc ttttctgagt tcgctggatg gattagcatt agatgatttg
     3961 gttttcatag attctgctag tcaaatctat cagttattac agaagcgaaa atttgtgaca
     4021 aatttagata accaaaccaa tcacttggta tttgtagaag agacagattt tgaaagaaac
     4081 actttgactc tttctaatta tttctataaa ctaactaact gttatccact ttcaagagaa
     4141 caaaaaaagc tttatcaact tttaggagat gttaattcgg aatatgcttt aaaatatatg
     4201 tcaagcttta tattgaaatt tcttcgcaag gatagtgtta agcaaaaacg tacagatatt
     4261 tttatccaag ctttagaatt acttggatat attagcttaa accaagatac tacctatcgt
     4321 ttaaatgcta aacttgatgt tgaagcttta aagatttact taacataaaa aaagactgga
     4381 agtttgtcca gtctttttta gtcaactttt gtcatcaacc agagaaaata gggggcacca
     4441 attattgata caaccaatcc agtgggtata cctgtaccaa ctaagaggag acgacctata
     4501 gtatctgata ccaataggat aatagcacca ataagaagac agctaggtag tataatttta
     4561 tggttatttc ctaatagtcg tctacttaag tgcccagcga gtaagcctat aaaagtaata
     4621 ttaccaatta agacgacgct aatagcagca aggctacttg ccagcatcaa agtatatatt
     4681 ctttcctttt ttaaatgaag ccctagagcg agagcagtct gctcgtttag gttcataata
     4741 ttaaggtggc gactgcgact atatgctaat ccccataaca aaatgagaat cgggataaaa
     4801 gtgataatgg tagtccagga actaattgta atttttccac taagccattc aacgatcata
     4861 tccatttttt gattattaga ctgactaata atagaaatca taacgcctga aatcattgta
     4921 gatattccga taccagtgat gattaatcgt gtagggctaa tattgtgatt tcttctgtat
     4981 gacataagat agacaagaaa aatagttacc aaccctccaa acatagcaaa taggggtaaa
     5041 aagtataata tagttggatt gctaacatta aatgttccta tggttagagc gattactaat
     5101 ccagctccag cattgatacc aagaataccg gaatcagcta gtggattttt ggttaatgtc
     5161 tgtaatagca acccagcaat tcctaaagaa gctcccccaa agatacagac aataatacga
     5221 ggtaaacgaa tgttagtaat gataaaaagg aaggcatcat cagattttcc acttattagt
     5281 tttaaaacgt caataacgga agtattagca tagccaagtg aaattgatac tagtattaat
     5341 aatagtagga taatgacaga tgatattaaa acaaaaggag ctttattttt ttgaatcata
     5401 gatttttccc ttttctaatt aaccatagga aacatggaag ccctacgata ctgattatag
     5461 aactaattgg tgtctctgaa gggggattta taatacgaga tgataagtct acccagatca
     5521 tgaaagtagc ccctgaaaat cctattaaag gtaataacag acgataatct ttgggaataa
     5581 aaagcttaat aaaatgtgga ataatcaatc caatgaaaga aacggttcct atcaacgcga
     5641 ctgatgaagc cgataaaaac aaaacaataa gtaatagaaa cgcagtcatt aattgagttt
     5701 tttgtcccaa tgctttggcc acagattcat taaggctcaa gatagttaat tgatgtgcta
     5761 ggagttgtga aataagtaaa cctaaaataa tgataggtgc tattatgatt agcattttcc
     5821 agttaatttg ggataaacca cctgcttgcc aaccgataac agttcgattg agtttaaagt
     5881 ataatgtaac cacttgtcca acagacgtaa aaagtgtgga gatcatcgct ccagctaaaa
     5941 tgagtcgtag ttggtggtaa cccttttgct ttgtataaga gagtgtgaag actaaaagac
     6001 aagaaatgac agaccctagt aagcagacaa tgagaatcgt actatagtgc atgctcccta
     6061 aaaaagcata tgcaacaaca agggctaacc cagctccggc gttaatacct agtagtccag
     6121 gatcagcaat ggcgtttcga gtgactccct gcataatagc acctgcttgt gacatagctg
     6181 cacctactaa gatagctgca attatgcgtg gtagtctaat gtcaaaaata acatcctgag
     6241 cattagagtt ttttaaggga ttttttaaaa cagttattaa atcactagtc ttgaaattga
     6301 tagcaccaaa tcgtaagctg gcgtaacaac caataagaaa tagtataagt gttatgaaaa
     6361 aaacaagcca aaaatgattt gacttatttt tttgaacgag tttttttgaa gctttaaaca
     6421 taagaggagc tttcttttaa tttgtatttt ctttgatagc ctttgtaaat gattttaatt
     6481 gagcttctaa agatagaggg tcagagaaat aaaacacgtc gtagttactt tctatgatgt
     6541 gccctttttt gacagctggt aaattcttcc agacatcact ttctttaagt gatgaagctg
     6601 cttttttagt cgttttgttt atattaacaa gggcataatc tccaacgtaa tcaccgattg
     6661 cttcttgcga aacggtaaac cacccttttt taaagacatc ttttttgact ttttctgggg
     6721 cagcataacc tagtgaatca tagattagtt ctccaccgcg tccaaaatta ttaccatata
     6781 aatagatatt tttatcataa aaatccataa tagtaaaagt agtgttaggc tttaagatat
     6841 ggtgtaaatc ttttttggca gcgagagttt tagttttcca ttggctaacc cactgattag
     6901 cttctttttc tttaccgaat actttcccca aggctggcat catatctaaa taattttgtg
     6961 caccatattt aataactaaa gttggtgcaa tttttttcag agtattgatg tttggatctt
     7021 gatcgaaaac catgattaaa tcaggttttt gtgcggcaat agcttctgta tcatcagcag
     7081 ttaatttttt agcttctttc agttgcttac caaaaacggg gctatctttt tctaagtcta
     7141 aactgtaact tgaaacatta acacctagtt ttaataaata cccagtgtaa gaatatgtaa
     7201 aattaattac ttttttcgga ttttcaggaa tttttccata ataggtgaag ccttcaattt
     7261 taggcatttt agaaatagtt gttttagtgc tttcttcttt agtttgttgt c
//
LOCUS       DATROD010000085         7310 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.085, whole genome shotgun sequence.
ACCESSION   DATROD010000085 DATROD010000000
VERSION     DATROD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.085"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            18..1388
                     /locus_tag="VBC74_001583"
     CDS             18..1388
                     /locus_tag="VBC74_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEO6942519.1"
                     /translation="MSKIVVVGTNHAGTAAIKTMLSNYGEANEIVTFDQNSNISFLGC
                     GMALWIGEQIDGPEGLFYSDKEQLESMGAKVYMNSPVLNIDYDKKEVTALVDGKEHVE
                     SYEKLILATGSQPIIPPIKGVEIQEGSREFKATLENLQFVKLYQNSEEVIEKLAKPGI
                     NRVAVVGAGYIGVELAEAFQRIGKEVTLVDVADTCMGGYYDRDFTDMMSKNLEDHGIR
                     LAFGQAVQAVEGDGKVERLVTDKETFDVDMVILAVGFRPNTELGAGKLDTFRNGAWVV
                     DKKQETSVKDVYAIGDCATIWDNSRDDINYIALASNAVRTGIVAAHNACGTELEGAGV
                     QGSNGISIYGLNMVSTGLTLEKAKQAGYNAVETGFNDLQKPEFIKHNNHEVAIKIVYD
                     KDSRVILGCQMVSHEDVSMGIHMFSLAIQEKVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(1509..2498)
                     /locus_tag="VBC74_001584"
     CDS             complement(1509..2498)
                     /locus_tag="VBC74_001584"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEO6942520.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPALF
                     DKAVGDAEDLSHALAFTSPKKIYAATYADCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFNGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALADKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVQLEQWLQENRDIDEQGLVDLF
                     ISVRDAAYSIINKKGATYYGIAVALARITKAILDDENAVLPLSVYQEGQYGDVKDVFI
                     GQPAIVGAHGIVRPVNIPLNDAELQKMQASAEQLKDIIDEAWKNPEFQEASKN"
     gene            2737..5196
                     /gene="gyrA"
                     /locus_tag="VBC74_001585"
     CDS             2737..5196
                     /gene="gyrA"
                     /locus_tag="VBC74_001585"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEO6942521.1"
                     /translation="MQDKNLVDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALEMLRDINKNTVDFQDNYDGSEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGESIDAVKLVMDNPDVTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYETGKGSIVLRSRTEIETTSNGKERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRIEGITAVRDESSREGVRFVIEVRRDASANVILNNLFKLTSLQTNFSFNMLAI
                     EKGVPKILSLRQIIDNYIEHQKEVIVRRTQFDKAKAEARAHILEGLLVALDHLDEVIT
                     IIRNSETDTIAQAELMSRFELSERQSQAILDMRLRRLTGLERDKIQSEYNDLLALIAD
                     LADILAKPERVVTIIKEEMDEVKRKYADARRTELMIGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGIQGTGVNNDDFVRELVSTSTHDTVLFFTNLGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLDEGETIQTIINARKEDVANKYFFFTTQQGIVKRTS
                     VSEFSNIRQNGLRAINLKENDELINVLLIDENEDVIIGTRTGYSVRFKVNAVRNMGRT
                     ATGVRGVNLREGDKVVGASRIVNGQEVLIITEKGYGKRTEASEYPTKGRGGKGIKTAN
                     ITAKNGPLARLVTINGNEDIMVITDTGVIIRTNVANISQTGRSTMGVKVMRLDQEAKI
                     VTVALVEQEIEDKSNIEDTKE"
     gene            5203..5946
                     /locus_tag="VBC74_001586"
     CDS             5203..5946
                     /locus_tag="VBC74_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEO6942522.1"
                     /translation="MRNKKKSHGFFNFVRWLLVVLLIIVGLALVFNKPIRNAFIAHQS
                     NHYQISRVSKKTIEKNKKSKTSYDFSSVKSISTESILSAQTKSHNLPVIGGIAIPDVE
                     INLPIFKGLGNTELSYGAGTMKENQIMGGPNNYALASHHVFGLTGSSKMLFSPLEHAK
                     KGMKVYLTDKSKVYTYTITEISKVTPEHVEVIDDTPGKSQLTLVTCTDPEATERIIVH
                     AELEKTGEFSTADESILKAFSKKYNQINL"
     gene            5962..6375
                     /locus_tag="VBC74_001587"
     CDS             5962..6375
                     /locus_tag="VBC74_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEO6942523.1"
                     /translation="MKLKAVHHIAIIVSDYEKSKDFYVNKLGFEIIRENHRPERHDYK
                     LDLRCGDIELEIFGNRLDDPEYETPPQRIGRPNWPREACGLRHLAFYVPDVEAYKVEL
                     ENLGIFVEPIRYDDYTGKKMTFFFDPDGLPLELHE"
     gene            6528..>7310
                     /locus_tag="VBC74_001588"
     CDS             6528..>7310
                     /locus_tag="VBC74_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017766755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEO6942524.1"
                     /translation="MSIKKLLFAGLAFILFTLASPAYAASDVQKVIDETYVQPDYVLG
                     YSLNQEQRAQTLQLLNYDESRDTKVKTLNTSSYAKIMNIADDASIQLYSSVKIKKLGS
                     NDTLAVNIVTPENITKVTEDMYRNAAVTLGIEHATISVAAPIKVTGESALAGIYYSLE
                     KNGASVSSENKQLAQEELSTLSGINAENKGKEGYDADKLNVALTDIKSAVAKGGSDLS
                     KDDIRKIVEETLKNYHLDNAVTENQINLIVNFAVNLSQSNVIK"
BASE COUNT         2417 a         1165 c         1494 g         2234 t
ORIGIN      
        1 aaaggagaac tcttaatatg agtaaaatcg ttgtcgtagg gacaaaccac gctggtacag
       61 cagcaattaa aacaatgctt agtaattatg gtgaagccaa tgaaattgta acatttgatc
      121 aaaattccaa tatctctttc ttaggatgtg gtatggctct ttggattggt gaacagattg
      181 atggtccaga aggattgttc tactctgata aagagcagtt ggaaagtatg ggagctaagg
      241 tttacatgaa ctctccagta cttaatattg actacgataa aaaagaagtt acagctttag
      301 tagatggaaa agaacatgtt gaaagttatg aaaaacttat tttagcaact ggttcacaac
      361 ctatcattcc accaattaaa ggtgttgaaa ttcaagaagg atctcgtgaa tttaaagcta
      421 ctcttgaaaa ccttcaattt gtgaaacttt atcaaaattc agaagaagtt attgaaaagt
      481 tagctaaacc tggtatcaat cgtgttgctg ttgttggggc tgggtatatt ggtgtagaat
      541 tagcagaagc tttccaacgt attggaaaag aagtaacact tgttgatgtt gctgatactt
      601 gtatgggtgg ttactatgat cgtgacttta ctgatatgat gagtaaaaac cttgaagacc
      661 atggtattcg tcttgctttc ggacaagctg ttcaagcagt tgaaggtgat ggtaaagtag
      721 aacgtcttgt tactgataaa gaaacatttg atgttgatat ggtgatctta gctgttggat
      781 tccgtccaaa tacagaactc ggtgcaggta aattagatac attccgtaat ggcgcttggg
      841 tagttgataa gaagcaagaa acaagcgtga aagatgttta tgctataggt gattgtgcga
      901 caatttggga taattctcgt gatgatatca attatattgc tcttgcttca aacgccgttc
      961 gtactggtat tgtagcagct cacaatgcat gtggtacaga gcttgaaggt gctggtgtcc
     1021 aagggtcaaa tggtatctca atctatggcc ttaacatggt gtcaactggt ttgactttag
     1081 aaaaagcaaa acaagctggg tacaatgcag ttgaaacagg ctttaatgac cttcaaaaac
     1141 cagaatttat caaacacaat aatcatgaag tagctattaa gattgtctat gataaagata
     1201 gccgagttat ccttggttgc caaatggtct cacacgaaga tgtatctatg ggaattcata
     1261 tgttctcact tgctattcaa gaaaaagtta ctattgaaaa gcttgctttg accgatatct
     1321 tcttcttacc acattttaat aagccatata actatattac aatggctgca ttaggcgcta
     1381 aagattaatt taaaattaaa aaggttaagc gaaatgctta gccttttatt tcatccttta
     1441 acaaggtcaa aaatgtaacg gcaaagaaaa agctaagata ttcctatcct agcttttttt
     1501 caactatgtt agttttttga tgcttcttgg aattctgggt ttttccaagc ttcgtcaata
     1561 atatctttta attgttcagc tgaggcttgc attttttgaa gttcagcatc atttaatggg
     1621 atattaactg gacgaacgat accatgtgca cctacgattg caggctgacc gataaagaca
     1681 tctttaacat caccgtattg gccttcttga tatacagata atggaagaac tgcattttca
     1741 tcatcaagga tagctttagt aatacgagca agtgcgacag caataccgta atatgtagca
     1801 ccttttttgt tgatgattga gtatgcagcg tcacgaactg aaataaacaa atcaacaagt
     1861 ccttgttcat caatatcgcg attttcttgt aaccattgtt caagttggac accagcaacg
     1921 ttagcatgtg accaaacggc aaattctgag tcaccgtgtt cacccatgat gtatgcgtga
     1981 actgaacgag catcaacacc aattttatct gctaaagctt gacggaaacg tgcagagtca
     2041 agtgaagtac ctgaaccaat aacgcgttcc ttagggaaac ctgagaattt ccaagttgaa
     2101 tatgtaagta catctacagg gttagccgct actaagaaga taccgttgaa acctgattca
     2161 acaacttgag ttacgattga tttgttaatt gctaagtttt taccaacaag gtcaagtcga
     2221 gtttcacctg gtttttgagg tgcgccagca gtaatgacaa caaggtcagc atctgcgcag
     2281 tctgcataag tagctgcgta gattttttta ggtgatgtaa atgcaagggc atgtgaaaga
     2341 tcttcagcat caccaacagc tttatcaaat aaagctggga tttcaatgat acctaactct
     2401 tgcgcaatac cttggttaac aagtgcaaaa gcgtaagaag aacctacggc accatcacca
     2461 acgagaataa cttttttatg ttgtttagtt gcagtcattt ctaaacatct ccttaaatat
     2521 ttttgggaag tttcccatac acctatattc tatcattttc aatgaaagaa gtcactaata
     2581 aaatgtgaaa aaatttgcaa acaaattgaa atatcataga aaactattga aaacgctgag
     2641 tgtcttgata tttttgatac cgatttatca tcgcaatttt acctaaatta tggtacaatg
     2701 taagagactt acagtgaaga aaaggggcat tttttaatgc aagataaaaa tttagtagat
     2761 gttaatctaa ctagtgaaat gaaaacgagt tttatcgatt acgccatgag tgtcattgtt
     2821 gctcgtgcac ttccagatgt tagagatggt ttaaaacctg ttcatcgtcg tattttatat
     2881 ggtatgaatg aactaggtgt gacacctgat aaacctcata agaaatcagc acgtattact
     2941 ggtgatgtta tgggtaaata ccatccacat ggtgattcat ctatttacga agcaatggtg
     3001 cgtatggcac aatggtggtc atatcgtcat atgctcgttg atggtcatgg aaactttggt
     3061 tcaatggatg gggatggtgc tgccgcacag cgttatacag aagcacgtat gagtaaaatt
     3121 gctcttgaga tgcttcgtga tatcaataaa aatacagttg attttcaaga taactatgat
     3181 ggcagtgaac gtgaacctct tgttttacca gcgcgttttc ctaatttatt agtcaatggt
     3241 gcaactggta ttgctgtagg tatggcaaca aatattccac cacataatct aggtgagtct
     3301 atagatgctg tcaaattggt tatggataac cctgatgtta ctacacggga gttaatggaa
     3361 gttattcctg gtccagattt tcctactggt gccttggtga tgggacgttc aggtattcac
     3421 cgtgcctatg aaactgggaa gggatcaatt gtcttacgtt cacggactga aattgaaaca
     3481 acttcaaatg ggaaagagcg tattgttgtc acagagtttc catatggcgt taataaaaca
     3541 aaagtacacg agcatattgt acgtttagca caagaaaagc gcattgaagg tattactgct
     3601 gttcgtgatg aatcaagtcg tgaaggcgtt cgttttgtta ttgaagttcg tcgtgatgct
     3661 tcagcaaatg tgattttaaa taatcttttc aaattaacta gcttgcagac aaattttagc
     3721 ttcaatatgc ttgctattga aaaaggtgtt ccaaaaattt tgtcattacg acaaatcatt
     3781 gataactata ttgaacacca aaaggaagtt attgttcgtc gtactcagtt tgacaaagct
     3841 aaagcagaag cccgtgctca tatcttagaa ggtttacttg tggcgcttga tcacttggat
     3901 gaagttatca caattattcg taatagtgag acagacacca ttgctcaagc agagttgatg
     3961 tcacgttttg aattatcgga gcgtcagagt caagctattt tagatatgcg tcttcgtcgt
     4021 ttaactggat tagaacgcga taaaatccaa tcagaataca atgacttact agctttaatt
     4081 gctgatttag cagatatctt agctaagcct gaacgtgttg ttaccattat aaaagaagag
     4141 atggatgagg ttaagcgtaa gtatgctgat gcccgtcgta cagaattaat gattggtgaa
     4201 gttttatcac ttgaagatga agaccttatt gaggaagaag atgttcttat tactctctca
     4261 aacaagggat atatcaagcg tctcgctcaa gatgagttta gagcacaaaa acgtggagga
     4321 cgcggtattc aagggactgg agttaataac gatgattttg ttcgtgaatt ggtttcaaca
     4381 agtactcacg atactgtgct tttctttaca aatttagggc gtgtgtatcg tttgaaagca
     4441 tatgaaattc cagaatatgg acgtacagct aaaggcctcc ctatcgttaa ccttttaaaa
     4501 cttgacgaag gtgagacaat tcaaacaatt attaacgcta gaaaagaaga tgtcgctaat
     4561 aaatatttct ttttcacaac tcagcaaggt atagtgaaac gtacaagtgt ttctgaattt
     4621 agtaacattc gtcaaaatgg acttcgtgct attaatttga aagagaatga cgagttgatt
     4681 aacgtcctct tgattgacga aaatgaagat gttattatcg ggacacgtac aggctactct
     4741 gttcgcttca aagtgaacgc tgtacgtaat atgggacgca cagcgactgg ggtacgaggc
     4801 gttaatcttc gtgaaggtga taaagttgtg ggagcttctc gtatcgttaa tggacaagaa
     4861 gtactaatca tcactgaaaa aggttatggc aagagaacag aagcttcgga atatcctaca
     4921 aaaggccgtg gtggtaaagg aataaaaacc gctaatatta ctgctaaaaa tgggccatta
     4981 gcacgcttag tgacgattaa tggaaatgag gacatcatgg tcattacaga tacaggtgtt
     5041 attattcgaa ccaatgttgc taatatttct caaactggtc gttctaccat gggtgttaaa
     5101 gtgatgagac ttgatcaaga agctaaaatc gtcactgttg cgctagtgga acaagaaatt
     5161 gaagataagt ctaatataga ggatacaaaa gaataggaag ttatgcgtaa taaaaaaaaa
     5221 tcacatggtt tctttaactt tgttagatgg ttattggttg tattgttaat tattgttggg
     5281 ttggctttgg tgttcaataa accaattcgc aatgctttca tagctcatca atcaaatcat
     5341 tatcaaattt cacgagttag taaaaaaaca attgaaaaaa ataagaaatc taaaacaagt
     5401 tatgactttt catcagtcaa atctattagt acagaatcga ttttatctgc tcaaacgaaa
     5461 tcacataatt tgcctgtaat aggagggatt gctataccag atgtcgaaat caatctccca
     5521 attttcaaag gactgggcaa tacagagttg agttatggtg caggaacgat gaaagaaaac
     5581 cagattatgg gaggcccaaa taactatgcc ttagctagtc accatgtctt tggtttgaca
     5641 ggttcttcta agatgctctt ttcaccttta gaacatgcta agaaaggtat gaaagtttac
     5701 ctcactgata aaagcaaagt atacacttac actattactg aaatttcaaa ggtaacacca
     5761 gaacatgttg aggttattga tgatacacct ggcaagagcc aactcacttt agtgacctgt
     5821 acggatccag aagccacaga acgtattatt gtgcatgctg aattagaaaa aacaggtgaa
     5881 ttttcaacag cagatgagtc gatattaaaa gcattttcga aaaaatataa tcaaattaat
     5941 ctctagaagg tagttagaat tatgaaatta aaggctgttc atcatattgc gataattgtt
     6001 tctgactatg aaaagtcaaa agacttctat gttaataagc taggatttga aattattcgt
     6061 gaaaaccatc gtccggagcg tcatgattat aaattagatt tacggtgcgg tgatattgag
     6121 cttgaaatat ttggaaatcg tcttgatgat ccagaatatg aaactcctcc tcagagaata
     6181 ggtaggccga attggccacg tgaagcatgt ggtttacgac accttgcctt ttacgttcca
     6241 gatgtggaag cgtacaaggt agaactggag aatttaggaa tttttgtaga gcctattcgt
     6301 tacgatgact atactggcaa aaagatgacc tttttctttg atccagatgg tttaccttta
     6361 gaattacatg aataataaac aaagttgcgt ggatacgcaa ctttgtttaa tttactgaat
     6421 aattttatat tcaagaagtt ttttaaagaa ataagtaagt taatcatggt ataatgtaag
     6481 gtatgaaaag aattgaaaag gaattatttt gaaatgagga tactaaaatg agtattaaaa
     6541 agctattgtt tgcaggcctt gcttttattt tatttaccct agcgagtcca gcatatgctg
     6601 catcagatgt tcagaaagtt attgatgaaa cttatgttca acctgattat gtcttaggtt
     6661 attcgctaaa tcaagaacag cgtgcacaga cattacaact tctgaattat gatgaatcta
     6721 gagacacgaa agttaaaacg ctaaatacta gctcatatgc taaaataatg aatatagctg
     6781 atgatgcaag catccaatta tattcatctg ttaaaataaa aaaattaggg tctaacgata
     6841 ccttagcagt aaacattgtt acacctgaga atattactaa agttacagaa gatatgtatc
     6901 gcaatgctgc agttacatta ggtattgagc atgcaacgat atcagtggct gctccaataa
     6961 aagtaactgg ggagagtgct cttgcaggta tctattattc attagagaaa aatggagcaa
     7021 gtgtatcatc agaaaataaa caactagcac aagaagagtt aagtacttta tctggtatta
     7081 atgctgagaa taagggtaag gaaggttatg acgccgacaa gcttaatgta gctttaacgg
     7141 atattaagtc agccgtagct aaaggaggat ctgatctttc aaaagatgat attcgaaaaa
     7201 ttgttgagga aacattgaaa aattatcatc ttgataacgc tgtaacggaa aatcaaataa
     7261 atttaattgt taattttgca gttaatcttt cacaaagtaa tgtcatcaaa
//
LOCUS       DATROD010000086         7162 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.086, whole genome shotgun sequence.
ACCESSION   DATROD010000086 DATROD010000000
VERSION     DATROD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7162
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.086"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(2..631)
                     /gene="udk"
                     /locus_tag="VBC74_001589"
     CDS             complement(2..631)
                     /gene="udk"
                     /locus_tag="VBC74_001589"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEO6942525.1"
                     /translation="MRKKPIIIGVTGGSGGGKTSVSRAILSNFPDQKIIMIEHDSYYK
                     DQSHLTFEERVKTNYDHPLAFDTNLMIEQLNELIEGRPVDIPVYDYTKHTRSDRTIRQ
                     EPQDVIIVEGILVLEDQRLRDLMDIKLFVDTDDDIRIIRRIKRDMEERDRSLDSIIEQ
                     YTEVVKPMYHQFIEPTKRYADIVIPEGVSNIVAIDLINTKVASILNEAK"
     gene            776..1858
                     /locus_tag="VBC74_001590"
     CDS             776..1858
                     /locus_tag="VBC74_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEO6942526.1"
                     /translation="MITKFPDQWQDKLTQRQFDDLTDIQNKLFQPITDGDNILGISPT
                     GTGKTLAYLFPTLLKLQPKKSQQLLILAPNSELAGQIFDVTKEWAEPLGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTAGRVFELVKLKKIKMMNINTIVLDEFDELLGDSQYHFV
                     DNIINRVPRDQQMIYISATNKLDNSKLADNTITIDLSDQKLDTIKHYYITVDKRERTD
                     LLRKFSNIPDFRGLVFFNSLSDLGACEERLQFNRASAVSLASDINIKFRKVILEKFKN
                     HDISLLLGTDLVARGIDIDNLEYVINFDIARDKETYTHRSGRTGRMGKEGCVITFVTH
                     KEELKQLKKYATVAELVLHNQKLHLK"
     gene            complement(1891..3144)
                     /locus_tag="VBC74_001591"
     CDS             complement(1891..3144)
                     /locus_tag="VBC74_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEO6942527.1"
                     /translation="MKKLIIGIFSLIIIAILAWQGFSFLKHKEIKLQQAVVEKEIRIA
                     EKTVEVVKRQKTERVLFLEPKGYDKSLSADILKWNQKSFEHKKFYDNQYIILRPQLAD
                     SNFANVKKLSIYQILYQKEKGSMFQKSSRLLRTYLLDQNKKPFELDELLAHNISGFKA
                     ILENIAPGTQLKEHDSNKEFLKTGRVTDGLDVKDGKLIINDLKLPLDKLYNVIDESYL
                     KSSDLDLVSNLKAKAPRVALTFDDGPNEKTTPKALEILKRYNAKATFFIMGQSAVGHT
                     DILQRMHAEGHEIGNHTWDHPNLTKLPAEKIKEEIHKTNDLIMKATGQKPVYLRPPYG
                     ATNATVKTVTGLKEMLWSVDTEDWKNHNTQAMMTNIKKQLRPGGVILMHDIHQTSIDA
                     LPTIMDYLTTQGYYFVTVGELYSTP"
     gene            complement(3284..4711)
                     /locus_tag="VBC74_001592"
     CDS             complement(3284..4711)
                     /locus_tag="VBC74_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEO6942528.1"
                     /translation="MTKEYQNYVNGEWKSSVNQIEILSPIDDSSLGFVPAMTREEVDH
                     AMKAGREALPAWAALTVYERAQYLHKAADIIERDKEEIATVLAKEISKAYNASVTEVV
                     RTADLIRYAAEEGIRLSTSADEGGKMDASTGHKLAVIRRQPVGIVLAIAPYNYPVNLS
                     GSKIAPALIGGNVVMFKPPTQGSVSGLVLAKAFAEAGLPAGVFNTITGRGSEIGDYIV
                     EHEEVNFINFTGSTPVGQRIGKLAGMRPIMLELGGKDAGIVLADADLDNAAKQIVAGA
                     YDYSGQRCTAIKRVLVVEEVADELAEKISENVAKLSVGDPFDNATVTPVIDDNSADFI
                     ESLVVDARQKGAKELNEFKRDGRLLTPGLFDHVTLDMKLAWEEPFGPILPIIRVKDAE
                     EAVAIANKSDFGLQSSVFTRDFQKAFDIANKLEVGTVHINNKTGRGPDNFPFLGLKGS
                     GAGVQGIRYSIEAMTNVKSIVFDMK"
     gene            complement(4861..6594)
                     /gene="ptsP"
                     /locus_tag="VBC74_001593"
     CDS             complement(4861..6594)
                     /gene="ptsP"
                     /locus_tag="VBC74_001593"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEO6942529.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVEDTNAEEARL
                     DVALQASQDELSVIREKAVESLGEEAAAVFDAHLMVLSDPEMINQIKETIRAKQVNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITERVQDGQ
                     LIAVNGITGEVIIEPTEAQISAFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNENGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAIFEEEKANLLADGVAVAEGIEVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALSECSTMEEV
                     IELQKEYVDFD"
     gene            complement(6599..6862)
                     /locus_tag="VBC74_001594"
     CDS             complement(6599..6862)
                     /locus_tag="VBC74_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEO6942530.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAIEETMTKEGLA"
BASE COUNT         2239 a         1404 c         1133 g         2386 t
ORIGIN      
        1 tttattttgc ttcatttaag atgctagcca ctttggtatt gattaaatca atagcgacaa
       61 tattacttac tccttctgga attacaatat ccgcataacg ctttgttggt tcgataaatt
      121 gatgatacat aggttttaca acttctgtat actgttcaat aatactatct aaacttctat
      181 cgcgctcttc catatcacgc ttaatacgac gaataatacg aatatcatca tctgtatcaa
      241 caaataattt gatatccatt aaatcacgca aacgctgatc ttctaatacc aaaattccct
      301 caacaataat gacatcctga ggttcttggc ggatagttct atcactacgg gtatgttttg
      361 tgtaatcata aaccggaata tcgaccggac gcccctcaat caattcgttc aattgctcaa
      421 tcatcaaatt ggtatcaaaa gcaaggggat gatcataatt ggtttttaca cgttcttcaa
      481 aagtcaaatg actttgatct ttatagtaag aatcatgttc aatcatgata attttttgat
      541 ctgggaaatt tgataaaatc gcccgagaaa cactggtttt accaccacca gatccacctg
      601 taacaccaat aataataggt tttttacgca tttgtcactc caaattaaat ttatcctcta
      661 tctattgtac tataaactta ttttttttaa agtctaaatt ttattgaaaa cgctctaaaa
      721 ataaaaagca tgttataata acaatgacaa catataaaat aacaagagga ttcttatgat
      781 aacaaaattt ccagaccaat ggcaggataa attaacccag cgtcaatttg atgatctaac
      841 agatattcaa aataaacttt ttcaacctat tactgatggt gataatatcc ttggaattag
      901 ccctacaggt actggtaaaa ctctagcata ccttttccca acgcttctta agttgcaacc
      961 taaaaagagc caacaactct taattttagc tccaaattca gaattagctg gacaaatctt
     1021 cgacgttaca aaagaatggg ctgaaccatt aggactgaca gcacaactat tcctttcagg
     1081 gtctagtcaa aaacgccaaa ttgagcgact aaaaaaagga ccagaaatcc ttataggaac
     1141 tgctggacgc gtatttgaac ttgtgaaact aaaaaaaata aaaatgatga acattaatac
     1201 tatcgtcctt gatgagttcg atgaattact cggcgactca caatatcatt ttgtggacaa
     1261 tattattaat cgcgttcctc gtgatcagca aatgatttac attagtgcca ccaacaaact
     1321 ggacaatagt aaacttgccg ataacactat aacaattgac ctatctgatc aaaaacttga
     1381 tactataaaa cattattata ttacagttga taaaagagag cgtactgact tacttcgtaa
     1441 attttcaaat attcctgatt ttagaggtct tgtctttttt aacagcttat cagacttagg
     1501 tgcatgtgaa gaacgtttac aatttaatag ggcctcggca gtttctttag caagcgatat
     1561 taatattaaa ttccgtaaag ttatcttaga gaaatttaaa aaccatgata tttcattact
     1621 cctaggaact gatttggtag ctcgtggcat tgatatcgac aacctagaat atgtgattaa
     1681 ttttgatatc gcacgcgaca aagaaacata tacacatcgt tctggacgaa ctggtcgtat
     1741 ggggaaagag ggatgtgtta ttacatttgt aacacataaa gaagagctca aacaacttaa
     1801 aaaatacgcc accgttgcag aacttgtact tcacaatcaa aaattacact taaaataaaa
     1861 aaagaaacaa gtaacttgtt tcttttttta ttaaggcgtt gaatataatt cacctacagt
     1921 aacaaaataa taaccctggg ttgtcagata atccataatt gtaggcaaag catctatgga
     1981 tgtctggtga atatcatgca tcaaaatcac tcccccagga cgtaattgtt ttttgatatt
     2041 ggtcatcatt gcttgtgtat tatgattttt ccagtcttct gtatcaactg accacaacat
     2101 ttctttcaat cctgtcactg tttttacagt agcatttgtt gctccgtaag ggggacgtaa
     2161 gtacactggt ttttgtcctg ttgctttcat gattagatca ttagtcttat gaatctcttc
     2221 cttgattttc tccgctggta gtttggttaa attaggatga tcccatgtat gattaccaat
     2281 ctcatgacct tcagcatgca tcctttgtaa aatatctgtg tgccctacag cactttgtcc
     2341 cattataaag aaagtcgctt tagcattata tcttttaagg atttctaagg ctttaggagt
     2401 cgttttttca tttggtccat catcaaaggt aagtgcaaca cgtggtgctt tagcttttaa
     2461 gttagaaact aaatctagat cacttgattt taaatatgac tcatcgatca cattatataa
     2521 tttatcaagt ggtaatttta aatcattaat aattaactta ccatctttaa catctagccc
     2581 atctgttact cttccagttt ttaaaaattc tttattgcta tcatgttcct ttagttgagt
     2641 tcctggagct atattttcta aaatagcctt aaatccagat atattatgag ctaaaagctc
     2701 atctaattca aaaggtttct tattttgatc aagtaagtag gttctcaaaa ggcgacttga
     2761 tttttgaaac atacttcctt tttctttttg atataagatt tgataaatgg ataatttttt
     2821 aacatttgca aagttagaat ctgctaattg aggacgtaga ataatgtatt gattgtcata
     2881 aaatttttta tgctcaaaac ttttttgatt ccactttaaa atatcagcgc ttagactttt
     2941 gtcatatcct ttaggttcaa gaaaaagaac acgttcagtt ttctgtcttt tcaccacttc
     3001 taccgttttc tcggctatgc gaatttcttt ttcaacaaca gcttgttgaa gttttatttc
     3061 cttatgtttc aaaaacgaaa aaccttgcca agcaagaatc gctataataa ttagactaaa
     3121 aatacctata attaatttct tcatataatg actcttttgt tattttttat gacaattata
     3181 tcacaaaaat aaaaccactt tagaagatta tataattctt tatttttaat gtaaaagagg
     3241 tttataaaac gactcaactg acaatcgttg agtcgtttta catctatttc atatcaaaaa
     3301 caatcgattt tacatttgtc attgcttcaa ttgaatatct gataccttga acacctgcac
     3361 cagatccttt gagtcctaag aatgggaagt tatcaggacc acgtccagtc ttattgttaa
     3421 tgtgaactgt accaacttca agtttatttg ctatatcaaa tgctttttgg aaatcacgtg
     3481 taaagactga tgattgtaat ccaaaatcag atttgttggc aatagcaaca gcttcttctg
     3541 catccttgac acgaataatt gggagaattg gtccaaaagg ctcttcccaa gctagtttca
     3601 tatctaaagt aacatgatca aacaatcctg gagttaatag acgaccatca cgtttaaatt
     3661 cattcaattc tttcgcacct ttttgacgtg catctactac taagctttca ataaagtcag
     3721 ctgaattatc atcaataacc ggtgtcaccg ttgcattatc aaatggatca cctactgata
     3781 attttgctac attttcagat attttttctg ccaattcatc tgcaacttct tcaacgacaa
     3841 gcacacgctt aattgccgta cagcgttgtc cagagtaatc ataagcacct gcaacgattt
     3901 gtttagcagc gttatcaagg tcagcatcag ctaagacgat acctgcatcc ttaccgccca
     3961 actcaagcat aattggacgc attcctgcca acttaccaat acgttgtcca actggcgttg
     4021 atcctgtaaa gttaataaaa ttaacttctt catgctcaac aatgtaatct ccaatctcag
     4081 aaccgcgtcc tgtaatagta ttaaagacac ctgctggaag acctgcttct gcaaaagctt
     4141 ttgctaaaac aagtcctgag actgaacctt gtgttggtgg tttaaacatc acaacgtttc
     4201 caccaattag agctggcgca atttttgatc ctgagaggtt aacagggtaa ttataaggtg
     4261 cgattgctaa aacgatacct actggttgac gacgaataac agccaactta tgacctgttg
     4321 aagcatccat ttttccacct tcgtcagctg aagttgataa acgaattcct tcttctgctg
     4381 cataacgaat aagatcagct gtccttacaa cctcagttac tgaagcattg taagctttag
     4441 aaatttcttt tgctaaaaca gtagcaattt cttctttatc acgttcaata atgtctgcgg
     4501 ctttatgaag gtattgtgca cgttcatata ctgttaaagc agcccaagct ggtaaagcct
     4561 cacgacccgc tttcatagca tgatcaactt cttctcgagt catcgctggc acgaatccca
     4621 atgaagaatc atcaattggt gacaaaatct caatctgatt aacagatgat ttccattcgc
     4681 cattgacata attttgatat tcttttgtca aagtaaatct ccttttacat tgtaataggt
     4741 ttattctatc actttatcta tttatttcaa ttatctgaca tatttttctt agaaacttaa
     4801 caatatccaa actttcaata aaaaagtaga gattttttaa tccctacttt tttattattc
     4861 tcagtcaaag tcaacgtatt ctttttgaag ttcgataact tcttccatag ttgaacactc
     4921 tgaaagagca cgattagcgt attcttccat tttagctgtg tccaattttt tcattaagct
     4981 acgtgtacga agtactgagg ttgctgacat tgagaattca tccaatccca taccaacaag
     5041 aagtggaacc gcagtttgat caccggccat ctcaccacac atacctgccc atttaccttc
     5101 agcatgagca gctttgataa cgttgttaat caaacgaagg attgatggat tgtatggttg
     5161 atagaggtat gaaacttgct cattcatacg gtccgcagcc attgtgtatt ggataagatc
     5221 attagtacca attgagaaga aatcaacttc ttttgcaaat tggtctgcaa gcatagctgc
     5281 cgctgggatt tcaatcataa taccaacttc aatgccttca gctactgcta caccgtcagc
     5341 taacaagttc gctttctctt cttcaaatat agctttagca gcacggaatt ctttaagcaa
     5401 agcaaccatt gggaacatga tgcgtagctg tccatgaact gaagcacgaa gaagtgctcg
     5461 gatttgtgtg cggaacattg catcaccagt ttcagaaatt gaaatacgca atgcacggaa
     5521 tcctaagaat gggttcatct cttttggaag atcaaagtaa ggaagctctt tatctccacc
     5581 gatatccatt gtacgaacaa caactggttt accattcata ccctcaagaa ctgctttata
     5641 tgcttcatat tgctcatctt ctgttgggaa atcttgtgaa tccatatata agaactctgt
     5701 acggtaaaga ccaactgctt ctgctccatt ttcgttaaca ccctcaacat ctttaggtgt
     5761 accgatatta gcagctaatt caaaatgttt accatcagca gtaactgttt gtgcatcctt
     5821 aagaagtgcc cattcagctt tttgcttggc ataagcttca cctgctgcct tgaaggctga
     5881 aatttgagct tcagttggtt caatgataac ttcaccagta ataccattaa cagcaatcaa
     5941 ctgaccatct tgaacacgtt cagtaatatc atttgtacca agaacagctg cgatttcgag
     6001 tgtacgtgcc ataatagctg agtgacttgt acgccctcca atatttgtta caaaagcttt
     6061 aacaaattgt ttatttaatt gagctgtatc agaaggtgtc aaatcgtgtg caataacgat
     6121 tgattcttca ttgatagttg ccgggtttgg taatttaaca cccaaaagat gtgctaagac
     6181 acgtttggca acatcacgga tatctgctgc acgttcttgc atgtatgggt tatcttccat
     6241 accttcaaag atagtgatga acatatcagt cacttcttta agacctgttt ctgcatttac
     6301 ttgttttgca cgaatagtct ctttaatttg attaatcatt tctgggtcag aaagaaccat
     6361 taaatgagca tcaaatacag ctgcggcttc ttcgcctaag ctttctactg ccttctcacg
     6421 gataacagaa agctcgtctt gtgaagcttg gagtgcaaca tcaaggcgag cttcttctgc
     6481 gtttgtatct tcgactgtta cagtctcaaa tgacaaatcc ggttggacaa gtagatatgc
     6541 tttagcaact gcaacaccat cagaggctgc gattccttta agcatttctg tcattttctt
     6601 atgccaatcc ttcttttgtc attgtttctt cgatagctgc aattgcatca tctgcatctg
     6661 caccttctgc tgaaatagta acatcagcac cttgaccaac accaagactc ataacaccca
     6721 tgattgattt aaggtttact gctttacctt tgtaatcaag cgtgatgtct gaagcaaatt
     6781 tgctagctgt ttggacaagc aaagtagctg gacgtgcgtg aatacctgtt tctgcaacaa
     6841 tgtggaaatc ttttgaagcc atattatggt tctccttttg tcatttgtaa atatttgagt
     6901 cacctattgg taacccttac aagttaaaag tataacatat tgctttttat ttttcaagat
     6961 taaatgtctc aaacagaaaa aaacttgaat gttttccaca gcttttcata ttaaaaatga
     7021 gagtcttaga atttaaaaat agaattctaa aaatgatttt tttatgttct tctgaaagga
     7081 attggaaaac aggctgaaag cttttaaact aaccctaaaa acgaaatata gtgtctcatt
     7141 ttattttaag acactatata tt
//
LOCUS       DATROD010000087         7016 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.087, whole genome shotgun sequence.
ACCESSION   DATROD010000087 DATROD010000000
VERSION     DATROD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.087"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            71..1354
                     /gene="aroA"
                     /locus_tag="VBC74_001595"
     CDS             71..1354
                     /gene="aroA"
                     /locus_tag="VBC74_001595"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEO6942531.1"
                     /translation="MKLLTNANTLKGTIRVPGDKSISHRAIIFGSISQGVTRIVDVLR
                     GEDVLSTIEAFKQMGVLIEDDGEIITIYGKGFAGLTQPNNLLDMGNSGTSMRLIAGVL
                     AGQEFEVTMVGDNSLSKRPMDRIALPLSKMGARISGVTNRDLPPLKLQGTKKLKPIFY
                     HLPVASAQVKSALIFAALQTKGESLIVEKEQTRNHTEDMIRQFGGHLDIKDKEIRLNG
                     GQSLVGQDIRVPGDISSAAFWIVAGLIIPNSHIILENVGINETRTGILDVVSKMGGKI
                     KLSSVDNQVKSATLTVDYSHLQATHISGAMIPRLIDELPIIALLATQAQGTTVIADAQ
                     ELKVKETDRIQVVVESLKQMGADITATADGMIIRGNTPLHAASLDCHGDHRIGMMIAI
                     AALLVKEGEVDLSGEEAINTSYPNFLEHLEGLVNA"
     gene            1347..1859
                     /locus_tag="VBC74_001596"
     CDS             1347..1859
                     /locus_tag="VBC74_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEO6942532.1"
                     /translation="MPKVLLGFMGVGKTSVANCLENEVIDMDSLIEKHIGMSISRFFT
                     EEGEASFRALESQFLNELLKKKNEGLVIASGGGIVLLEENRRLLTLNRHNNILLTGSF
                     EVLYHRIKKDEKNRRPLFLNHSKEEFYDIYQKRMLLYSGLSDMIIDTDYLTPQKIATV
                     IGESLVNLSN"
     gene            1916..3289
                     /locus_tag="VBC74_001597"
     CDS             1916..3289
                     /locus_tag="VBC74_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO6942533.1"
                     /translation="MSRNNYGQLNHHEELRYNYLLKNIHYLNEREKMEFQYLHYKKTA
                     VRPQRRTESPPTNSYYEEPYSDSYYQDDDFYSEPQLTSQGLPIYQEERAPKKKKQRAR
                     KEKQRVKVMAPFPPKAITPPRKKKKFKGFLKFIGIILLIVLSGMVFMFVKGMRDVNNG
                     KSHYSPAIIEDFKGKDAVDGTNILILGSDKRVSERSTDARTDTIMVANVGNKDNKVKM
                     VSFMRDLLVNIPNYSTEGYYDMKLNASFNLGEQDNHKGAEYVRQTLKNHFDIDIKYYV
                     MVDFETFADAIDTLFPNGVKINAKFGLVGGQSADSVKVPDDLRMKNGVVPSQKIKVGI
                     QYMDGRTLLNYARFRKDDDGDFGRTQRQQQVMRAIVSQIKDPRRLFTGSAAIGKAYAL
                     TSSNLSYSFVLTDGIPILSDAKNGIKQMTIPREGDWVDDYDQYGGQGLTIDFAKYKKI
                     LKKMGLR"
     gene            3390..4745
                     /gene="rlmD"
                     /locus_tag="VBC74_001598"
     CDS             3390..4745
                     /gene="rlmD"
                     /locus_tag="VBC74_001598"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO6942534.1"
                     /translation="MLHKNDIIETEISDISHEGMGIAKVDGFVFFVENALPGEIIKMR
                     VLKLRKRIGYGKVEEYLTTSPHRNEGLDYTYLRTGIADLGHLTYEQQLLFKQKQVADN
                     LYKIAHISDVLVEPTLGMTIPLAYRNKAQVPVRRVDGQLETGFFRKNSHTLVSIEDYL
                     IQEKEIDALINFTRDLLRKFDVKPYDEEQQSGLIRNLVVRRGHYTGQLMLVLVTTRPK
                     IFRIDQMIEKLVSAFPSVVSIMQNINDRNSNVIFGKEFRTLYGSDTIEDQMLGNTYAI
                     SAQSFYQVNTEMAEKLYQKAIDFSDLNSEDIVIDAYSGIGTIGLSVAKQVKHVYGVEV
                     VEKAVSDAKENATRNGITNSTYVADSAENAMAKWLKEGIKPTVIMVDPPRKGLTESFV
                     YSAAQTKADKITYISCNSATMARDIKLFEELGYHLVKIQPVDLFPMTHHVECVALLVK
                     A"
     gene            4853..5074
                     /locus_tag="VBC74_001599"
     CDS             4853..5074
                     /locus_tag="VBC74_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942535.1"
                     /translation="MPTMQLLDELVLPKDITIADVSQSIQEFEIERDALQEFFDDMLK
                     TYTTYQEMKDYIKSREANTPKQETEEKSL"
     gene            5192..5929
                     /gene="aphA"
                     /locus_tag="VBC74_001600"
     CDS             5192..5929
                     /gene="aphA"
                     /locus_tag="VBC74_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEO6942536.1"
                     /translation="MKKVLVSSLLVLGITITLQTVVEAKGPKVAYTQEGMTALSDTNK
                     DKVTTISIDEIQKSLEGKKPITVSFDIDDTLLFSSQYFQYGKEYVTPGSFDFLHKQKF
                     WDLVAKRGDQDSIPKEYAKKLIAMHQKRGDKIVFITGRTRGSMYKEGEVDKTAKALAK
                     DFKLDKPIAVNYTGDKPKKPYKYDKSYYIKKYGSDIHYGDSDDDIHAAREAGARPIRI
                     LRAPNSTNLPLPEAGGYGEEVLENSAY"
     gene            6250..6768
                     /locus_tag="VBC74_001601"
     CDS             6250..6768
                     /locus_tag="VBC74_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEO6942537.1"
                     /translation="MRVYENKEELKKEISKTFEKYIMEFNNIPENLKDKRIDEVDRTP
                     AENLSYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSIYAMIDLLSEEELFEAHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(6840..>7016)
                     /locus_tag="VBC74_001602"
     CDS             complement(6840..>7016)
                     /locus_tag="VBC74_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001875973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEO6942538.1"
                     /translation="SDIPDLQKQLETAYQVPYSFALGIYISTIESIILNWIDHDFTEE
                     PEEIARYITSVVRI"
BASE COUNT         2475 a         1015 c         1361 g         2165 t
ORIGIN      
        1 ataaataaaa aattcaaaat atagcatata ttatgataca ataatagcaa aatagatttt
       61 gggagttatc atgaaattac tgactaatgc aaatactctc aagggaacca tccgtgttcc
      121 tggcgataaa tcaataagtc acagggctat tatttttggg agtatatctc agggagttac
      181 ccgaattgtt gatgtcttaa gaggcgagga tgttttatca actatagagg cttttaaaca
      241 aatgggggtt ctaattgaag acgatggcga gataatcact atctatggca aaggatttgc
      301 aggtttaaca caaccgaata atctcttaga catgggaaac tctggtactt caatgcgtct
      361 gatagcaggt gtgttagctg gtcaagagtt tgaagtgacg atggttggag acaatagttt
      421 gtcaaagaga cctatggatc gtatagcatt gcctttaagc aaaatgggag ctagaatttc
      481 gggagtaact aaccgtgatt taccaccatt aaaacttcaa ggtacaaaaa aattaaaacc
      541 tattttttac catttacccg tagcttctgc acaagtaaaa tcagctttaa tttttgcggc
      601 tttacaaact aagggagaat ctctcattgt agaaaaagag caaacaagga atcatactga
      661 ggacatgatt cggcaatttg gtggacattt ggatattaaa gataaagaaa ttcgactcaa
      721 tggcggtcag agcttagtag gacaagatat aagagttcct ggtgatattt cgagtgctgc
      781 cttttggata gtagcgggtc ttattattcc taacagtcat attattttag aaaacgtagg
      841 tataaacgag acacgaactg gaatccttga tgtcgtcagt aaaatgggtg gaaaaattaa
      901 gttatcttcg gttgataatc aagtaaaatc agcaaccctt acagttgatt actctcatct
      961 acaagctact catatttctg gggctatgat tccacgtctg attgatgaat tacctattat
     1021 tgctctttta gcaacacaag ctcaaggcac cacagtcata gcagatgctc aggaattaaa
     1081 ggtgaaagag acagatcgga ttcaggtcgt tgtcgaatcg ctaaaacaga tgggtgctga
     1141 tattactgct acagcagatg gtatgattat acgtggtaat acccctcttc acgctgcttc
     1201 acttgattgt catggagatc atagaatagg aatgatgata gctattgctg ctctcttggt
     1261 caaagaaggc gaagtagatt tatctggtga agaagctatt aatacaagct atcctaactt
     1321 tttagaacat ttggagggat tagttaatgc ctaaggtatt attgggattc atgggagttg
     1381 gcaagactag tgttgccaac tgcttagaaa atgaagtaat tgatatggat agtctgattg
     1441 aaaaacatat tggtatgtca atttcccgat tttttacaga agaaggagaa gcctcctttc
     1501 gtgctttaga atctcaattt ttaaacgaac ttttaaagaa aaaaaatgaa ggacttgtga
     1561 tagctagtgg aggaggtatt gtactccttg aagaaaatcg tcgtctttta actttaaatc
     1621 gtcataataa tattctttta acaggaagtt ttgaagtctt atatcatcgt ataaaaaaag
     1681 atgagaagaa tagaagacca ttatttttaa accattcaaa agaagaattt tatgatattt
     1741 atcaaaaacg catgttactc tatagtggtt tgtctgatat gattattgat accgattatc
     1801 ttacaccaca aaaaatagct actgtaattg gagagtcact ggttaattta tctaactaag
     1861 ttatcacagt ttggtagtat gaactattat tattgaaagt aaatcacctg aaaatatgtc
     1921 aagaaataat tacggtcagc taaatcatca tgaagagcta cgatataact atcttttaaa
     1981 aaacatccat tatcttaatg aacgtgaaaa gatggaattt cagtaccttc attataaaaa
     2041 aacagcagta cgaccacaga gaaggacaga aagtccacca actaattctt attatgagga
     2101 accttattct gattcttatt atcaagatga cgatttttac tcagagcctc agctaacctc
     2161 acaaggttta ccaatttatc aagaagagag agctccgaag aagaaaaaac aaagggccag
     2221 aaaagaaaaa cagagggtga aggttatggc gcctttccct ccaaaggcta taaccccccc
     2281 tagaaagaaa aaaaaattta agggtttcct aaagttcata ggtattatat tactaattgt
     2341 tttatcaggt atggttttca tgtttgttaa aggaatgcgt gatgtgaata atgggaagtc
     2401 acattatagc cctgcaatta ttgaagattt taagggaaaa gatgctgttg atgggactaa
     2461 tatcttaatt ttaggtagtg ataaacgtgt cagcgagaga tctactgatg cgagaacaga
     2521 taccataatg gttgctaatg ttggtaacaa ggataataag gttaagatgg tgagttttat
     2581 gcgagatctc ttagtcaata ttcctaatta tagtacggaa ggttactatg atatgaaatt
     2641 aaatgcgtct tttaatttgg gagaacaaga taatcataaa ggagcagagt atgttcgaca
     2701 aacgttgaaa aatcattttg atattgatat aaaatattat gttatggttg actttgagac
     2761 atttgcagat gctattgata cactgtttcc taatggtgtc aagattaatg ctaaatttgg
     2821 acttgtcggt ggacaatctg ctgattcagt taaagttcct gatgatttaa ggatgaaaaa
     2881 tggcgttgta cctagccaaa aaattaaggt tgggattcag tacatggatg gaaggaccct
     2941 tctaaattat gctcgtttca gaaaagacga tgatggggac tttggtcgaa cacaacgtca
     3001 acaacaagtt atgagagcta ttgtctctca aataaaagac ccacgtcgtc tctttacagg
     3061 ttctgctgct atcggtaaag cctatgcttt aacttcaagt aatttatctt atagctttgt
     3121 cctaacagat ggcattccaa ttttatcaga tgctaaaaat ggtattaagc aaatgacaat
     3181 tccaagagaa ggagattggg tagatgatta tgaccagtat ggtggtcaag ggttaacaat
     3241 tgattttgct aagtacaaaa agatcctaaa gaaaatggga ctgcgttaac atttttaatt
     3301 atgatataat agagtgattt attgtcactc tatttttatc tttagtgtat aaactaaaga
     3361 cttcaaatga tagatataaa aggaaaatta tgttacataa aaatgatatt attgaaactg
     3421 aaattagcga cattagccat gagggaatgg gtattgccaa agtagatggc tttgttttct
     3481 ttgttgaaaa tgctcttcca ggtgagatta ttaagatgcg cgtcttgaaa ttacgtaagc
     3541 gtataggata tggaaaagtt gaggaatatt taacgacttc tcctcaccga aatgaaggat
     3601 tagactatac ctatttacgt acagggattg ctgatttggg acatcttact tatgaacaac
     3661 agctgttatt taaacaaaaa caagtagctg ataaccttta taaaattgct catatatctg
     3721 atgttttagt agaacctact cttggtatga caatcccttt ggcatatcga aataaagctc
     3781 aggtaccagt tcgtcgtgtt gatggacagc tagagacagg ttttttcaga aaaaattcac
     3841 atactctggt atccattgaa gattatttaa ttcaggaaaa agagattgat gctctgatta
     3901 attttacacg agatttatta agaaaatttg atgttaaacc atatgacgaa gagcagcaat
     3961 ctggtttaat tagaaatctc gttgttcgtc gaggacatta tacagggcaa ttaatgttgg
     4021 ttttggtgac aacacgtcct aaaatattcc gcattgacca aatgattgaa aagttggtgt
     4081 ctgcctttcc ttctgttgtt tcaattatgc aaaatattaa tgatcgtaat agcaacgtta
     4141 tttttgggaa agaatttcgt actctatatg gttctgatac tattgaggac caaatgttag
     4201 gaaatactta cgctatatca gcccaatctt tttaccaagt taatactgaa atggctgaaa
     4261 aactgtacca aaaagcaatc gatttttcag atttaaactc agaggatatc gttattgatg
     4321 cctattcagg tataggaaca attggcttat ctgttgctaa acaagtaaaa catgtttatg
     4381 gtgttgaagt tgttgaaaaa gcagtaagtg atgccaaaga aaatgctact cgaaatggga
     4441 taactaacag tacctatgtt gctgattcgg ctgaaaatgc aatggcaaag tggcttaaag
     4501 aaggtataaa accaactgtt ataatggttg atccaccacg aaaaggatta acggagagtt
     4561 ttgtatattc tgctgcccaa acaaaagcag ataaaatcac ttatatttct tgtaattcag
     4621 cgacaatggc gcgtgatatc aaactatttg aagaattagg gtatcattta gtaaaaattc
     4681 aaccagtaga cttattccca atgacacatc atgtggagtg tgtggctctg ctcgtaaaag
     4741 cctagaaact ttttaacgaa aggaatatta aaaagcctta gataatcgac tgacctatta
     4801 ccaaaaaatc gaaggggctc tacttgccat tgcacaaaca ccaggaaata gtttgccaac
     4861 tatgcagctg ttagatgagt tagttcttcc aaaagacatt accatagcag atgtcagtca
     4921 aagtatccaa gaatttgaaa tagaaagaga tgcccttcaa gaattttttg acgatatgct
     4981 gaagacttat acaacctacc aagaaatgaa agattatatt aaatctcgtg aagcgaacac
     5041 tcctaagcaa gagacagaag aaaaatcact ttgaagactg tagacttaac ataagtaaaa
     5101 aataaccata tatgatatcg ctttcattct tatgtttttt gtgataaact aattaaagaa
     5161 ataattttta tactttatta tggaggttct tatgaagaaa gtgttagtga gtagtctttt
     5221 ggttttaggg attacgataa cgttacaaac agtagttgag gctaaggggc caaaagtagc
     5281 ttatacacaa gagggaatga ctgctctttc ggacacaaat aaagataaag tcactactat
     5341 ttctattgac gagattcaaa aaagcttaga aggtaagaag ccgattactg ttagttttga
     5401 tattgatgat acactgcttt tcagtagtca atattttcaa tatggtaaag aatatgtaac
     5461 tcctggatcg tttgattttc ttcataaaca aaaattctgg gatcttgttg caaaacgagg
     5521 agatcaagat tccattccca aagaatatgc taaaaaatta attgctatgc atcaaaaacg
     5581 aggagataaa attgttttta taacaggtag gacaagaggg tcaatgtata aggagggcga
     5641 ggttgataaa acagctaaag ccttagctaa agattttaaa ttagacaaac caattgctgt
     5701 aaattataca ggcgataaac ctaaaaagcc atacaaatat gataaatcat attatattaa
     5761 gaaatatggt tcagacattc attatggaga tagtgatgac gatattcatg cagctaggga
     5821 ggccggtgct agaccaatta gaattttaag agcacctaat tctacaaatc tacctttacc
     5881 agaagctgga ggctacggtg aagaggttct cgaaaattca gcttactagt atctttatta
     5941 gttaacatag tattatctat caatttttag atttgtaata agaagtgcta aaaaagtagc
     6001 aaatcaatga tatgctaata agggaagttg gagtagttta gattgattat taatgactga
     6061 tcttctctcc tctcggtaac aagaagtttt tctaacaaat gcctgtctaa aaaaattgaa
     6121 tcataatttt ttaataactc ttttggcgtg aacaaggaat ttgtaatcag tcatctaatt
     6181 ctataataca atactttggg gttgtttccg tatcattatt aataatatgt atcagaaagg
     6241 agatgttttt tgagagttta tgaaaataaa gaagagttga aaaaagaaat aagtaaaaca
     6301 tttgagaaat acattatgga atttaataat attccagaga atctaaaaga taaaagaatt
     6361 gatgaagttg atagaactcc agcagaaaac ctttcttatc aggttggctg gaccaacttg
     6421 gttcttaaat gggaagaaga tgaaagaaag ggacttcaag taaaaacacc atcggataaa
     6481 tttaaatgga atcaacttgg tgaattatat cagtggttca cagataccta cgctcattta
     6541 tctctgcaag agttgaaagc aaaattaaat gaaaatatta attctatcta tgcaatgatt
     6601 gatttgttga gtgaggaaga attatttgaa gcgcatatga gaaagtgggc tgatgaagcg
     6661 actaaaacag cgacttggga agtgtataag tttattcatg taaatacggt tgcacctttt
     6721 ggaactttca gaactaaaat cagaaaatgg aagaagatag tattataaat tatattttta
     6781 actttaaaaa atttcataaa aatggttacc aaagacgata gttttttcta atacaaatat
     6841 taaattctca cgacagatgt aatatatctt gctatttctt caggctcttc agtaaagtca
     6901 tgatctatcc aatttaaaat aatactttca attgttgaga tataaatacc aagagcgaaa
     6961 gaatatggaa cttgatatgc tgtttccagt tgtttttgta agtctggtat atcgga
//
LOCUS       DATROD010000088         6936 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.088, whole genome shotgun sequence.
ACCESSION   DATROD010000088 DATROD010000000
VERSION     DATROD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.088"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            57..218
                     /locus_tag="VBC74_001603"
     CDS             57..218
                     /locus_tag="VBC74_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEO6942539.1"
                     /translation="MGGIGAHKFYAGKIGQVFLYIIFSITFIPGIIDLIEALMALGKQ
                     TDEYENIIV"
     gene            complement(391..600)
                     /locus_tag="VBC74_001604"
     CDS             complement(391..600)
                     /locus_tag="VBC74_001604"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6942540.1"
                     /translation="MSRIGKCIDNAPTESFFGFFKTESYHLKKYNSYDELVNDVARYI
                     EFYNTQRYQSKLNNLTPLEFRNQVA"
     gene            complement(726..986)
                     /locus_tag="VBC74_001605"
     CDS             complement(726..986)
                     /locus_tag="VBC74_001605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6942541.1"
                     /translation="MMNILGISSVIRRVSHACTKAGDRFYEENILNREFTATAHNQKW
                     CTDVTYLQYGLGAKAYLSAIKDLYNGSIIAYEISHNNEIHLL"
     gene            complement(1008..1208)
                     /locus_tag="VBC74_001606"
     CDS             complement(1008..1208)
                     /locus_tag="VBC74_001606"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEO6942542.1"
                     /translation="MPIQALCQLLKGSRSGYYKWLNRQKTDFETKNTKLMAKIKELHR
                     LYNGILGYRRMTTFINRQLGTT"
     gene            complement(1370..1774)
                     /locus_tag="VBC74_001607"
     CDS             complement(1370..1774)
                     /locus_tag="VBC74_001607"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEO6942543.1"
                     /translation="MIYSTLAKEQGVQGYLDGKGSLRDICKWYDISSRSVLQKWIKRY
                     TSGEDLKATSRGYSRMKQGRQATFEERVEIVNYTIAHGKDYQAAIEKFGVSYQQIYSW
                     VRKLEKNGSQGLVDRRVKGLESRPDLTEIEQL"
     gene            1774..2040
                     /locus_tag="VBC74_001608"
     CDS             1774..2040
                     /locus_tag="VBC74_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001881173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEO6942544.1"
                     /translation="MAYFRKRDNGWEYRISYKHIIGNILLCNITSTKYQQVINHYSQT
                     HVQDTVKRFNIHVKACVSMAVHEGYIEKNFCLFTKVKAKNKGRK"
     gene            2043..2657
                     /locus_tag="VBC74_001609"
     CDS             2043..2657
                     /locus_tag="VBC74_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6942545.1"
                     /translation="MKFLETNEYLRLIKICKEKSQHQSYAALYLIAKTGMRFAECLGL
                     TVNDIDYTNKYLSINKTWDYHFNQRYLPTKNKSSIRNIPIDNDTLFFLHEFTKNKNDR
                     LFDKLSNNAVNKTIRKITGREVRVHSLRHTFASYLISISQVLDHENLNITLEVYAHQL
                     QEQKDRNDKLNQRNLGRIWGKIALNRYLHAMNMSPAGIEPAITP"
     gene            complement(2620..2691)
                     /locus_tag="VBC74_001610"
     tRNA            complement(2620..2691)
                     /locus_tag="VBC74_001610"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2656..2658),aa:Arg,seq:cct)
     gene            complement(2802..3149)
                     /gene="rplS"
                     /locus_tag="VBC74_001611"
     CDS             complement(2802..3149)
                     /gene="rplS"
                     /locus_tag="VBC74_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEO6942546.1"
                     /translation="MNPLIQSLTEGQLRSDIPEFRAGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(3733..4929)
                     /locus_tag="VBC74_001612"
     CDS             complement(3733..4929)
                     /locus_tag="VBC74_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEO6942547.1"
                     /translation="MIKRFDNYAQYVLVLGFSLLIGGVVGAITAVFGRVLLFLTAFRS
                     DYIAYLLPFLSIVGLFIVFVYQKFGGKSVKGMGLVFEVGHGNEETIPKRLVPLVILTT
                     WLTHLFGGSAGREGVAVQIGATVSHYFQKYCRLQNASQLFLVMGMAAGFAGLFQTPLA
                     ATFFAIEVLVVGRLMVSYILPSLIAALTANFVSHSLGLEKFSHSIATSMALTPDIILK
                     LLVLGLCFGLCGNLFAYLLAKAKLIASSRLLNPYKRIFTLGLLATFLLFIFHFGRYSG
                     LGTNLIEASFTNKNLYDYDWLLKLCLTVITLAAGYQGGEVTPLFAIGASLGVIIAPIL
                     GLPVILVAALGYTSVFGSATNTLLGPILIGGEVFGFANTPYFVIVCLVAYSISHAHTI
                     YGAQSR"
     gene            complement(4922..5197)
                     /locus_tag="VBC74_001613"
     CDS             complement(4922..5197)
                     /locus_tag="VBC74_001613"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEO6942548.1"
                     /translation="MELEEIRQEIDEIDQQLVSLLETRMGLILEVIAFKKKHRLPVLD
                     NNRENEVLNNVLKKVQNHQFDDVIRATFKDIMTESRVYQKENIVDGD"
     gene            complement(5274..5717)
                     /locus_tag="VBC74_001614"
     CDS             complement(5274..5717)
                     /locus_tag="VBC74_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEO6942549.1"
                     /translation="MALAKIVYASMTGNTEEIADIVADKLRDLGLDVEVEECTMVDAA
                     DFEDADIAIVATYTYGDGDLPDEIVDFYEDLAEVDLSGKVYGVVGSGDTFYDYFCKSV
                     DEFEAQFALTGAQKGADCVKVDLAAEDEDIENLEAFAEEIASKLN"
     gene            complement(5776..6798)
                     /locus_tag="VBC74_001615"
     CDS             complement(5776..6798)
                     /locus_tag="VBC74_001615"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEO6942550.1"
                     /translation="MTQAVLKELAKAELHCHLDGSLSLPAIRKLANMADIILPNSDKE
                     LRKYVIAPAQTESLVDYLKTFEFIRPLLQTKEALRFAAYDVARQAALENVIYIEIRFA
                     PELSMDKGLTASDTVFAVLEGLADAQKEFNIVARALVCGMRQSSHKTTKEIIKHIVDL
                     APKGLVGFDFAGDEFSYPTDSLVDLIQEVKRSGYPMTLHAGECGCAKHIADSLNLGIK
                     RMGHVTALTGQRALIKRFVEEDAVAEMCLTSNLQTKAASSIQSFPYQELYDAGGKITI
                     NTDNRTVSDTNLTKEYSLFVTYFGTKIEDFLVFNQNAVKASFTSDSEKDTLLHKLQEN
                     YDSYLK"
BASE COUNT         2299 a         1369 c         1086 g         2182 t
ORIGIN      
        1 gttgtaaaaa taaagtctta tgctaacata gtagtttatg ttttattaac gttcttttgg
       61 gcggcattgg agcgcataaa ttttacgcag gaaaaatagg ccaggttttt ttatatatta
      121 tttttagtat tacctttatt cctggaatta tcgaccttat cgaagctttg atggctctcg
      181 gaaaacaaac tgatgaatat gaaaatatca tcgtttaatg aaacaaaaag cctcacgctc
      241 aatttttggt cgaggagagc gtgaggcaag acagtataag aaataagcat taaatggctc
      301 gttttcttgt agctatttta gcaaatttag aaaggttaag caatctgaac ggctccctgt
      361 caagtagaca gtcaaataat aaaagataag ttatgcaacc tgattcctga attctagagg
      421 agtcagattg tttaattttg attgataacg ttgtgtgttg tagaattcga tataacgtgc
      481 cacatcattg accaactcat cataagagtt gtatttctta aggtggtaag actcagtctt
      541 gaaaaaccca aagaaacttt cagttggtgc attatcaata catttgccaa tccgggacat
      601 ggataaggtc agaccagctt gttgtatgat ataacggtat tctttggaag tatattgact
      661 acctcaatcg ctatggatga taggtgtggc tcctggattg agctctagcc cctttttaat
      721 ggtcttcata acaagtggat ttcattgttg tgactaatct cataagcgat aatagaaccg
      781 ttatacaggt ctttaatcgc actgagataa gctttagctc ccagaccgta ttgaagatag
      841 gtgacatctg tgcaccattt ctggttatga gctgtggctg taaattcacg attaagaata
      901 ttttcttcgt aaaatctgtc accagctttt gtacaagcat ggctaacacg acgaatgact
      961 gaactaatcc ccagaatgtt catcaatcaa cgaatccgtt tcttgtttta agttgtccca
     1021 agttgacgat taataaatgt tgtcatacgg cgataaccta agataccatt gtagagtcta
     1081 tgaagttcct tgattttagc cattagcttt gtattttttg tctcaaaatc tgttttttga
     1141 cgattgagcc acttgtaata gcctgaacga gaccccttca agagttggca taaggcctga
     1201 ataggcacat tagattcctc atcgtaataa ttcttgatta cttggaactc cgctaaatgc
     1261 ttacctagtc ttaccgtctg tttcctcgtt tgatgtcttc taactttttt agtaaactaa
     1321 cctcgatttc taagagacga ttacgttcct ccaattgttt aatcttgagt taaagttgct
     1381 caatctcggt taaatcaggc ctactctcca accctttcac acgtctatca accaaacctt
     1441 gtgagccatt cttctcaagc ttacgcaccc aagaataaat ttgttggtag gaaacaccaa
     1501 acttctcaat agctgcttga tagtctttcc catgggcaat ggtgtagtta acaatctcta
     1561 cacgttcttc aaatgtggct tgccttcctt gtttcatacg gctatatcct ctactagtgg
     1621 ctttcaagtc ttcaccacta gtataccgtt ttatccactt ttggagaaca gagcgacttg
     1681 agatatcata ccatttacaa atatctctaa gagagccttt tccatcaagg tagccttgaa
     1741 caccctgttc tttagctaat gtggagtata tcaatggcgt atttccgtaa acgcgacaac
     1801 gggtgggaat atcgaatatc gtataaacat ataataggaa atatattact ttgcaatatt
     1861 acttccacta aatatcaaca agtcataaat cattatagtc aaacgcatgt gcaagataca
     1921 gtaaagcgtt ttaacataca tgtaaaagcc tgcgtatcca tggctgttca cgagggatat
     1981 atagaaaaaa atttttgttt attcactaag gttaaagcaa aaaataaggg gcgtaaataa
     2041 agataaaatt tcttgaaacc aatgaatact tacgcttgat aaaaatttgt aaagaaaaat
     2101 cacaacatca atcgtatgca gcgttatacc tcatcgctaa aactggcatg cgcttcgctg
     2161 aatgtttagg tttaacagtt aatgatattg attacactaa caaatattta tcaattaata
     2221 aaacttggga ttatcatttc aaccaaagat acctgccaac aaaaaacaaa agtagtatca
     2281 gaaatatacc aattgacaat gatacattat tttttttgca tgaatttaca aaaaataaaa
     2341 acgacagatt attcgacaaa ctttcaaata atgcagtcaa taaaactatc cgaaaaataa
     2401 ctggtcgcga ggtacgagtg cactctctca ggcatacatt tgcaagctat ctaatttcca
     2461 tctctcaagt tttagatcac gagaatttaa atatcacttt agaagtttac gcacatcaat
     2521 tacaagaaca aaaagatagg aacgataaac ttaatcagag aaatttgggg cggatttggg
     2581 gcaaaatagc tctaaaccgc tatttacatg caatgaatat gtcccctgcc ggaatcgaac
     2641 cagcaattac tccttaggag ggagttgtta tatccattga actaagggga cctagtaaaa
     2701 aaactgccca caggcagatt ttttacgtct tggttgtcca gttttaaaac atagttacta
     2761 tcctcaaaca accaagcatt tttaaaatct gatcatcaaa attaacgacg gatttcttta
     2821 atacgtgcag ctttaccttg caatgcgcgt aagtagtaaa gtttagcacg acgtacttta
     2881 ccataacgaa caacttcgat tttatcaaca cgaggagtgt gaattgggaa tgtacgctct
     2941 acaccgatac caccagaaat tttacgtact gtgtacattt ctgagattcc ttgaccttta
     3001 cgtgagataa caacaccttc aaagatctga atacgttcgc gagtaccttc aacaacttta
     3061 gcgtgaacac gtacagtatc accagcacgg aactcaggga tatcagaacg aagttgacct
     3121 tctgtcaaac tttgaattaa tggattcatt tttattctcc ttctcttact aatcttaagt
     3181 acttgtctca gcggattagc cgttttttgt gcgtccatta cacactctca caattctacc
     3241 aaattttaac ctgcttgtaa agctatttta tctagaagaa caaggtgtca tcctttagaa
     3301 tagttatttg tatattattg aaatttgatt tactgtttct aggttagctt ttttctgatt
     3361 tttgagagtt taattcattt aatattatat gattttttaa actacgtttt ctatattagc
     3421 tttcgtgtca cctgactctt tcccataaag ttgaaatcca catcaacttt ttatcttcta
     3481 atgcatttca ccatcgcgac ttaaatgctt gtagacatta tcctaaccta agttcttaca
     3541 cattcaattg gattttacag tattttaatg acaatcataa aatcaatttc tttgaagaaa
     3601 attatattag ccacataatt tttgcattca taaaatttac aaacttcatt tttataaact
     3661 ttaagagaat aaaaaagaga acggaatatt ccgttctata aactgataaa ctcttttaaa
     3721 actcatcact aactatctag attgagcacc ataaatcgta tgagcatgtg atatggagta
     3781 agcaacaaga catacaataa caaaataagg agtgttggca aagccaaaga cttcgccacc
     3841 tattaagata ggacctaaca gagtattcgt tgcactccca aaaacgctag tatagcctaa
     3901 agcagcaact aaaataactg gcaaacctaa aatgggcgca ataatcactc ctaaacttgc
     3961 tcctattgca aataagggag ttacttctcc tccctgataa cctgcagcca aagtaataac
     4021 tgtcaaacac aattttaaca accaatcata atcataaaga ttcttattag tgaagctagc
     4081 ctcaattaga tttgttccca gtcctgaata tcgtccaaaa tggaagataa acaataaaaa
     4141 agtagctaac aatccaagtg tgaaaatccg tttatatggg tttagtaacc tactagaagc
     4201 aattaattta gctttagcca ataaataagc aaataaattg ccacacagac caaaacatag
     4261 tcctaaaacc aataatttta gaataatatc tggtgttaac gccattgatg ttgctatact
     4321 atgagaaaat ttttctaaac ctaaagaatg tgagacgaaa tttgctgtca gagcagcaat
     4381 taaacttggt aatatatacg aaaccattaa acgacctact accaaaactt cgatcgcaaa
     4441 aaaagtagca gctaaaggag tctgaaataa accagcaaaa cctgctgcca ttcccattac
     4501 taggaaaagt tgagaagcgt tttgtaaacg gcaatatttt tgaaagtagt gtgaaacggt
     4561 agccccaatc tgtacagcaa ccccctcacg acctgcacta ccaccaaata agtgggttaa
     4621 ccaagttgtt aatatcacga gaggaacaag acgtttcgga atagtctcct cattaccatg
     4681 acctacttca aataccaatc ccatcccttt aacactctta cccccaaatt tttgatagac
     4741 aaaaactata aataaaccaa caatgctaag aaaagggagt aggtaagcta tataatcact
     4801 tctaaaggct gtcaggaata atagaacacg tccaaagact gctgtaatag ccccaaccac
     4861 accaccgatt agtaagctaa accctaaaac aagtacgtac tgagcataat tatcaaatcg
     4921 tttaatcacc gtctacaata ttctcctttt gataaacacg agattctgtc ataatgtctt
     4981 tgaaagttgc tcgtatcaca tcgtcaaatt gatgattttg gaccttcttc aatacattgt
     5041 taagcacttc gttttcacga ttattatcta aaactggaag tcggtgtttc tttttaaatg
     5101 ctatgacctc taaaattaga cccattctag tttctaaaag gcttaccaat tgctgatcta
     5161 tttcatctat ttcttgacga atctcttcta attccatatc tatatctcat attctagtta
     5221 caaaagatgg taaaaaagtt aaaaagacga ctcgtgttcg tctttttggt agcttagtta
     5281 agtttagaag caatttcttc agcaaaagct tctaagtttt caatgtcttc gtcttctgcc
     5341 gctaaatcaa ctttaacaca atcagcacct ttttgtgctc ctgttaatgc gaattgagcc
     5401 tcgaactcat caacagattt gcagaagtaa tcatagaaag tatcacctga acccactacg
     5461 ccgtaaactt taccagataa atcaacttct gctaaatctt cataaaagtc cacgatttca
     5521 tctggtaaat ctccatcacc atatgtataa gtggcaacaa ttgcaatgtc agcatcttca
     5581 aaatctgcag catcaaccat cgtacactct tctacttcaa catctagacc caagtcacgt
     5641 aatttatcag caacaatgtc agcaatttct tcagtatttc cggtcatgct agcataaaca
     5701 atctttgcta aagccatatc ttcctccact tgtaaattac cactattcta tcatattttt
     5761 aaagaatcgt gaaggttatt ttagatagct gtcatagttt tcttgtagct tatgtagtaa
     5821 agtatctttc tctgaatcac ttgtaaagga ggccttcaca gcgttttgat taaaaacaag
     5881 aaagtcttct attttcgttc caaaataagt tacgaaaaga ctatattctt tcgtcaaatt
     5941 tgtatcggaa acagtacgat tatctgtatt aattgtaatc ttacctccag catcataaag
     6001 ttcttgataa ggaaaacttt gaatacttga ggctgctttc gtttgtaaat tgctagttaa
     6061 acacatctct gctacagcat cttcttcgac aaatcgcttt attaaagccc tctgtcctgt
     6121 taaagctgtc acatgtccca tacgcttaat ccctaaattt aaactatccg caatatgctt
     6181 agcacaacca cactcacctg catgtaaagt catcgggtaa cctgagcgtt ttacttcctg
     6241 aataagatct actaaactat ctgttggata ggaaaattca tcacctgcaa aatcaaaacc
     6301 taccaggcct ttgggagcca aatcaacaat gtgtttaata atttccttgg tcgttttatg
     6361 agacgattgg cgcataccgc agacaagggc gcgtgcaacg atattaaatt ctttctgagc
     6421 atctgctaaa ccttctaaga ctgcaaagac tgtatcactt gcggttaaac ctttatccat
     6481 agagagttct ggcgcaaaac gaatttcaat ataaataaca ttttctaaag ctgcctgtct
     6541 agcaacatca taagcagcaa aacgtaatgc ttccttcgtt tgaagaagcg gtctgataaa
     6601 ctcaaacgtt ttaaggtaat ctaccaagct ttctgtttga gctggagcta taacatattt
     6661 tcttaattct ttatcactat ttggcagtat tatatcagcc atattagcca attttctaat
     6721 agctggtaaa gaaagtgagc catctagatg acaatgcaac tccgccttag ccaattcttt
     6781 taatactgct tgtgtcataa tttatctcct attaaaaagc atttttacta tattaacaaa
     6841 agttagctaa aaagcaatac atttacaaaa tattatcata aaaaagattg ttagttagca
     6901 gtatccgaat tttataaaaa aaataacata ccatca
//
LOCUS       DATROD010000089         6912 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.089, whole genome shotgun sequence.
ACCESSION   DATROD010000089 DATROD010000000
VERSION     DATROD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.089"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            282..1397
                     /locus_tag="VBC74_001616"
     CDS             282..1397
                     /locus_tag="VBC74_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEO6942551.1"
                     /translation="MSDDLISIQSSCLSIAKTFANFNIQRKRFMISTETEMDTFHKKD
                     DIDVWVGGKSYNPARSFRTRTINELTVIDFEEMFDILWLMLGDNLIKSFEVNVCGILF
                     ELGGNDIPSTFRQENIDPLINKWWYDNVSTEIIPNLIKKLKENPLFNIGFMVNDILER
                     MYKENIPKSYLTSVPLVISQKGRTTYSFSMTGGQQIDGVKFTQIYEDYMKLLSQGKDI
                     AELYQKYSKEELANLGINIYQSNDIERTEERTFDEIISWVSNPYATRPIQERHTIQLE
                     PTRFSLEDKKRIEEAAAQGLSEIDLIDLVDLYDINLDNTSVNRHIVGLLTNNTQVTYY
                     FQEQLNKELLSMAHALDNVQQAFIKLLSEEEIRKFAL"
     gene            1717..2166
                     /locus_tag="VBC74_001617"
     CDS             1717..2166
                     /locus_tag="VBC74_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6942552.1"
                     /translation="MSNKVQERRERKIKEAIKAKNWDEVTRLLQQEQSNAERRDRYHN
                     RRIKDETIASKNAKKSVRYDVIASSDLNPEEALILEELRQAIREAKASLSEIDSKIVE
                     MIAEQGSSYKETARYITEHYKKMSDVTVKSHYCKALKKLAPLLKAYR"
     gene            2368..2688
                     /locus_tag="VBC74_001618"
     CDS             2368..2688
                     /locus_tag="VBC74_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942553.1"
                     /translation="MAFKMKTTKGGYTTTLADKIISQKQPIYSLSTELEPQQRFEEGK
                     PTGEIVAYKAWFVQEGQDPFQVKFEETIELPAFQSMIQFDTLQACEVKYNIYFKANGI
                     KEVR"
     gene            2688..3788
                     /locus_tag="VBC74_001619"
     CDS             2688..3788
                     /locus_tag="VBC74_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEO6942554.1"
                     /translation="MTSQSRDVLNQSYLQSYLLQSLNMALGALMQGETSYTNSFNIVI
                     QADGFIFVPRLPCAYILDDDLYKKIFLIANASLYPQYTLLKQNATYFVPLETDDLHTQ
                     RGLFFPWKKGISERLVIPDLDKFSASLPQGKIPIMKYFELNLDKVNHWAIAGNSGSGK
                     SYALTYFLSVLKHMSDLIIIDPKFDTPSRWARENHVAVIHPVENRSKSDFVSQVNEQL
                     SQCATLIQKRQAILYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQISLLG
                     RATKIHLFLVSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPIG
                     HGTGIIQVIDNEHPYQVLPLLCPTYYTKRGIL"
     gene            3785..4390
                     /locus_tag="VBC74_001620"
     CDS             3785..4390
                     /locus_tag="VBC74_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942555.1"
                     /translation="MTNKTYFMRSFNLILYLFLFAPICSAFGWLFNYLSVQLTNITFV
                     NLDTVKSITDIISSVCHGFALISLLLALFLVGLEIIQRLKTDSLWNYIQSVYHTFLLR
                     HFLFQRERVQKISNLEHQTVTTINPIHNGFNRAVRKCIVDIRKDTITIFLKVPRDQQG
                     QKILKEMEAQLKEEIVSQHADYYFSSPIRVRNQLWFTGQKR"
     gene            4596..5201
                     /locus_tag="VBC74_001621"
     CDS             4596..5201
                     /locus_tag="VBC74_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEO6942556.1"
                     /translation="MAKEQRSTKWTFLFYEESAPENYLNILEELHIPFILSPWHDKDV
                     NRQTGEFKKSHKHGAFFFDSLKSYSQVSNIISDKLNGPAHVEVVMSPTGLFDYFTHAE
                     NPDKTPYNIEDIEVGCGFNLEKFLMEMNSSDFIHEVVDIIEENDFTEFEELVWYARAN
                     NTNLLGLIIERTYFFAKYLDSRRYNPNRLHNSNTEEKENNE"
     gene            5194..5442
                     /locus_tag="VBC74_001622"
     CDS             5194..5442
                     /locus_tag="VBC74_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6942557.1"
                     /translation="MNNEKLEQIEQLLQTLIKLIQIKEQNIPLKIIQQRELLKQLNIS
                     PNTLKTWEQKGLKRLEPPIEGTRTVFYLLDDIINFLQS"
     gene            5532..6797
                     /locus_tag="VBC74_001623"
     CDS             5532..6797
                     /locus_tag="VBC74_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6942558.1"
                     /translation="METETIIHNGKKVIKKIKKDKSISYTLKGTFLGKDIKTGKQVTT
                     TITAKTLKQLDRAIIQARLEFEKNGSTREKVIVIDTLEDLAEEWFKSYKTWVNSHNTL
                     NRVRGYLDNYIIPKFGDYKPDKIESADIQLWLNDLAKKSKESIESGIKRADKGSAKDF
                     GAVIHKLKDIFDYGITNYGLITNPVSTVKIPPKPKSNKQRIMVLHDDGLVIWLNYLES
                     LDNNRANRRFKLICNTLLASALRINELLALTIDDLDFENSSINVSKTLMWKTANKKDG
                     TKGEVICKATPKTDAGNRSVPVPPPIIEKLRDFHHEMNKYLELHNRHRTKLIFPTIYG
                     NYMCDRNERTTLKKRLVGLGLPNYGFHLFRHTHASMLLNAGTNWKELQVRMGHKSIST
                     TMDTYAELAPQRKLEAVGIYLEKIAELTQ"
BASE COUNT         2448 a         1261 c         1179 g         2024 t
ORIGIN      
        1 attattcaca agaaatacct actcgtatca aaaatttaag aaaaaaatac aatattacac
       61 aaagcgaact aggcaatgca ggtcaagtta gtcaagttga aagtggtaag cgaccaatta
      121 cgagttcgat gttagtttat ttaaatgctc taaccgcttc gagctatacg tatatcgttt
      181 ttggagagtt agatgagttt attgagaatc tgtttcatta ttttttcagt tctattttat
      241 atagagattt agaggctgta gatgaaaaac tatattcttt catgagtgat gatttaattt
      301 caatccaatc aagttgtcta agtattgcca agacatttgc aaatttcaat attcaaagaa
      361 aaagatttat gatttctact gaaactgaaa tggatacttt tcacaagaaa gatgacattg
      421 acgtatgggt tggtggaaaa agttataacc ccgctagaag ttttagaacc cgtaccatca
      481 atgaattgac tgtcattgat tttgaagaaa tgtttgatat tctttggttg atgttagggg
      541 ataatttaat taaatcattt gaagttaatg tctgtggtat cctatttgaa ttaggcggaa
      601 atgacatacc ctcaacattt aggcaagaaa acattgatcc tctcatcaat aaatggtggt
      661 atgataatgt ttcgacagaa attatcccta atctaataaa aaaactcaaa gaaaatccct
      721 tgtttaatat cggatttatg gtaaatgata tattagaaag aatgtataaa gagaatattc
      781 caaaatctta ccttacatcc gtcccattag ttatttctca aaaaggaaga acaacctatt
      841 cgtttagtat gactggtggt caacaaatag atggagtgaa attcacacag atatatgagg
      901 actatatgaa attactcagt caaggtaagg atatcgcaga gttatatcaa aaatattcta
      961 aagaagagtt ggcaaatcta ggcattaata tttatcaatc caatgatata gaaaggactg
     1021 aggaaagaac ttttgatgaa attatcagtt gggtttccaa cccttatgca acaagaccaa
     1081 ttcaagaaag gcacactatt caattagagc caacaagatt ttcactagag gataagaaaa
     1141 gaattgaaga agctgcagct caaggactaa gcgaaatcga ccttattgat ttagttgacc
     1201 tatatgatat taatttagac aatacaagcg tcaatcgcca tattgtgggg ttattgacta
     1261 ataacaccca agtaacatac tatttccaag aacaattaaa taaggagttg ctgtcaatgg
     1321 ctcacgcttt agataacgta caacaggcct ttattaaatt attaagtgaa gaggagatac
     1381 gaaaatttgc tctttaaaga ctaggtgaat cgcctagtct tttttgcgcc tcgttttaca
     1441 aattagtcta ttgaagtaaa aaaagaagta aaaaataaaa gaacattacg ccttatactg
     1501 aatatatcaa gaaaaactct tgatataaat tttaatagtc caaattgatg gccatcaaag
     1561 gacagattga aacaaatatg atgacatgac gataaaggtc atgtcttgca tagaattgtt
     1621 tcgctgtttc tttgttgtgc cttttggtat actcagcgtt caatatttgc tcattttgtt
     1681 tccttctgtc caaaggacag aaaggagctc atcattatga gtaataaagt acaagaacgt
     1741 cgtgagcgta aaatcaaaga agctattaaa gctaaaaatt gggatgaagt aactcgtttg
     1801 cttcaacaag aacaaagtaa cgcagaacgt cgtgaccgat accacaatag acgaatcaaa
     1861 gacgaaacaa tagctagcaa aaatgccaaa aagtctgtac gttatgatgt cattgcaagt
     1921 tctgatttga atcccgaaga agctttaatc ctagaggagt taagacaagc tattcgtgaa
     1981 gctaaagcat ccctatcaga aattgacagt aagattgttg aaatgatagc ggaacaaggt
     2041 tcaagctata aagaaacggc tcgctatatc actgaacatt ataagaaaat gagtgatgta
     2101 actgttaaat cccattattg taaagcgcta aaaaaattag ctcctttatt aaaagcttac
     2161 cgctaatccc tcaaatccag tgtctctgtc actggatttt ttgtaaaaaa aaattaaaaa
     2221 ttcaacaaat aactttgcgt ttagctgtct cattctcctt agtaatgagg aggtggtcag
     2281 ataacctaaa aaacaaagtc cagctcatga cccaaaccga gcctgtcgca ttcacactat
     2341 ctcttattca taaggaggat ttgccctatg gcgtttaaaa tgaaaaccac aaaaggtggt
     2401 tatacaacta cattggctga taaaattatc agtcaaaaac aaccgattta ctcacttagt
     2461 accgaacttg aaccacaaca acggtttgaa gaaggaaaac caaccggaga aatcgtagct
     2521 tataaagcat ggtttgtaca ggaagggcaa gaccctttcc aagtaaaatt tgaagaaacg
     2581 attgagcttc cagcttttca atccatgatt caatttgaca cactacaagc ctgcgaagta
     2641 aaatataaca tctacttcaa agcaaatggt atcaaggagg ttcggtaatg acttcacaat
     2701 ctcgtgatgt gctgaaccaa tcttacctgc aatcttacct gctccagagc ctaaacatgg
     2761 ctctgggggc tttaatgcag ggagaaacca gctacaccaa ttcttttaat attgttattc
     2821 aagcagacgg ctttatcttt gtccctcgtc taccatgtgc ttatatcctt gatgatgact
     2881 tgtacaagaa aatctttctg attgccaatg cttcacttta tccacaatat acgcttctaa
     2941 aacaaaacgc tacctatttt gtccctctag aaacagatga cttacacact cagcgtggat
     3001 tatttttccc ttggaaaaag ggaatttcag aacgtttagt cattccagat ttggataagt
     3061 tttcagctag cttgccacag gggaaaatcc ctatcatgaa gtactttgaa ctcaatttag
     3121 ataaggttaa tcactgggct attgctggaa attcaggttc tgggaaatcg tacgctctca
     3181 cttatttctt gagtgtacta aagcatatgt ctgacttgat tatcattgac cctaaatttg
     3241 ataccccaag tcgatgggca agagaaaatc acgttgctgt tatacaccct gttgaaaacc
     3301 gttcaaaatc agattttgtc tcacaggtca atgaacaatt aagtcagtgt gcaactctca
     3361 ttcagaaacg acaagctatc ttgtatgata atcccaatca tcaatttacc catctaacta
     3421 ttgttattga tgaagtcctc gctctatcag agggagtcaa taagaatatc aaagaagcct
     3481 ttttctccct actctcacag atttccttat taggacgtgc taccaaaatc catctgtttt
     3541 tggtaagcca gcgttttgac cacaatacta ttccaatttc agtgagggaa cagctaaacg
     3601 tattgttgca aattggaaat atcaatcaga aaactactca atttttattt ccagacctag
     3661 acccagaggg tattgttatt ccaataggac atgggacagg tatcattcaa gttatcgata
     3721 atgaacatcc ctaccaagtt ctgcccttac tctgtccaac ctactacact aaacgaggta
     3781 ttctatgacc aataaaacct actttatgag aagttttaat ctcatactat atttatttct
     3841 atttgcacct atctgttctg catttggatg gttattcaat tatctttccg ttcaattaac
     3901 aaatatcacg tttgtcaatt tagatacggt caaaagtatt acggatatca ttagttccgt
     3961 ttgtcatgga tttgcactta tttctcttct acttgcctta tttttggttg gattagaaat
     4021 aatacaacgg ttgaaaacag atagtctttg gaactatatc caatctgtct atcacacttt
     4081 tctgttgcga cacttcctgt ttcagcgtga acgagtacag aagataagca atcttgaaca
     4141 tcagacggtt actactatca acccaatcca taacggattt aaccgagctg ttcgtaagtg
     4201 tatcgtagat atacgaaaag ataccatcac tatttttcta aaagtcccta gagaccaaca
     4261 agggcaaaaa atcctaaaag agatggaagc acaattaaaa gaagaaatcg tcagccaaca
     4321 cgcagactat tatttctcct ctcctattcg agttcgtaac caattgtggt tcactggaca
     4381 aaaacgttaa tcccttgggg ctgtggcttg cgttaagcaa aagccagaca gccccttttt
     4441 atattctttt ctatcatgac ctattggggt gactacgacc cccaataggt caataatcaa
     4501 taatcacaaa aaattcaatt ttttcaaaaa aactttgcat acgacctgtc atttctcctt
     4561 agtaatagaa cccaaaagaa atgaggtaac tgcctatggc aaaagaacag cgctcaacca
     4621 aatggacttt tctcttctac gaggaaagcg ccccagagaa ctacttgaat atattagagg
     4681 aactccatat tccgtttatt cttagtcctt ggcacgataa ggacgttaat agacagacgg
     4741 gagagttcaa gaaatcgcac aaacacggtg ctttcttctt tgattcactg aaaagctatt
     4801 cacaagtatc aaatatcatc agtgacaaac taaacggtcc agcacatgtg gaggtagtta
     4861 tgtctcccac tggtttattt gactacttta cccatgcgga aaatccagat aagaccccct
     4921 acaacattga ggatattgaa gtcggttgcg gctttaattt agaaaaattc ttgatggaga
     4981 tgaactcctc ggattttata catgaagtag ttgatattat cgaagagaac gactttaccg
     5041 aatttgaaga attggtctgg tatgcccgag caaacaatac caatttatta gggcttatca
     5101 ttgaacgtac atatttcttc gctaaatacc tagattcacg aagatacaat cctaatcgac
     5161 tccataattc aaacacagag gagaaagaga ataatgaata acgaaaaatt agaacaaatt
     5221 gaacaattat tgcaaacact tatcaaactc attcaaatca aagaacagaa cataccacta
     5281 aagataatac aacagcgaga attgctaaaa cagctaaata tctccccaaa cacactaaaa
     5341 acttgggaac aaaaaggatt aaaaagatta gaacctccta tagagggaac tcgaaccgtc
     5401 ttctatctac tagatgatat tatcaacttt ttacaatcct aattctgaaa atttgtgcta
     5461 aaatagtttt atctaaaaga aagagtggta actattttag cctacttcaa tatccatgaa
     5521 gaaaggcaaa tatggaaact gaaacaatta ttcataatgg caaaaaagtc attaaaaaaa
     5581 tcaaaaaaga taagtccatc tcatacactt tgaaggggac atttctggga aaagatatca
     5641 aaactggtaa gcaagtcact actaccatta cagccaagac actaaaacag ttagatagag
     5701 caatcattca agcacgacta gaatttgaaa aaaatggctc aacacgagaa aaagttatcg
     5761 tcattgatac actggaagat ttagctgaag aatggtttaa atcatataaa acctgggtta
     5821 actcgcataa tactctgaac agagtaagag gttatcttga taattatatt atccctaaat
     5881 ttggagatta caaaccagat aaaattgaat ctgcggatat ccagctttgg ttaaacgatt
     5941 tggcaaagaa atcaaaagag tccattgaat ctggtataaa gcgtgctgat aaaggtagtg
     6001 ctaaagattt tggtgctgtt atccataagt tgaaagatat ttttgactat ggaattacaa
     6061 actatggact aatcactaat cctgttagca ctgttaagat accacctaag ccaaaatcaa
     6121 ataagcaacg catcatggta ttacatgatg atggtcttgt aatatggctt aattatcttg
     6181 aaagtttaga taacaataga gccaatcgta gatttaagtt aatttgtaat actctattag
     6241 cctccgcttt gcgaataaat gaattacttg cattgacgat tgatgaccta gattttgaaa
     6301 attcttctat taacgtcagt aaaacattga tgtggaaaac cgcaaataaa aaagatggga
     6361 caaaagggga agttatctgt aaagctacac caaaaactga tgcaggaaat cgctctgtcc
     6421 ctgtccctcc ccccataata gaaaaactca gagatttcca ccacgaaatg aacaagtatc
     6481 ttgaattgca caacagacat agaacaaaac tgattttccc tacaatctat gggaactata
     6541 tgtgtgacag aaacgagaga acaactctta agaaaaggtt agtaggtctt ggattaccaa
     6601 actatggttt ccatttattc cgacatactc atgcttcaat gctacttaat gctggaacaa
     6661 attggaaaga actccaagtc agaatgggac ataaatccat ttcaactaca atggatactt
     6721 acgctgaatt agcaccacaa cgaaagttag aagctgtggg gatttatctt gaaaagattg
     6781 ctgagctaac gcaataacta ctctaccatt cactctacct tttattatgt agcattctag
     6841 aaacattgat gtaccaacgt ttctaacagc taaaatagaa attatttaac tcttactgaa
     6901 gttaaataat tg
//
LOCUS       DATROD010000090         6692 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.092, whole genome shotgun sequence.
ACCESSION   DATROD010000090 DATROD010000000
VERSION     DATROD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6692
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.092"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..373
                     /locus_tag="VBC74_001624"
     CDS             <1..373
                     /locus_tag="VBC74_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEO6942559.1"
                     /translation="VGIIGYKMINGIHSPTNKNLEEHKDDPMNVAVSPLAMPLLAGPG
                     TIATAMGLSSGGLSGKLITILAFAILCVIMYVILISANEITKFLGKNAMTIITKMMGL
                     ILMTIGIEMLITGIKIGFHLT"
     gene            543..2444
                     /gene="mnmG"
                     /locus_tag="VBC74_001625"
     CDS             543..2444
                     /gene="mnmG"
                     /locus_tag="VBC74_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEO6942560.1"
                     /translation="MTHNFAENYDIIVVGAGHAGVEASLAASRMGCKTLLATINLEML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KALYAQTMKQTVEKQENLTLRQAMIDEILVEDGKVVGVRTATNQKFSAKSVVITTGTA
                     LRGEIILGDLKYSSGPNNSLASVTLADNLRDLGLEIGRFKTGTPPRVKASSINYEKTE
                     IQPGDEQPNHFSFMSRDEDYITDQVPCWLTYTNTLSHDIINQNLHRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRYTEEVYVQGLSTSLPEDVQVDLLRSIKGL
                     ENAEMMRTGYAIEYDIVLPHQLRATLETKVIAGLFTAGQTNGTSGYEEAAGQGLVAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGYEIGLVDEERYAIFKKRQMQFENELERLDSIKLKPVSETNKRIQELGFKPLTD
                     ALTAKEFMRRPQITYAVATDFVGCADEPLDSKVIELLETEIKYEGYIKKALDQVAKMK
                     RMEEKRIPPHIDWDDIDSIATEARQKFKKINPETLGQASRISGVNPADISILMVYLEG
                     RQKGRKNIN"
     gene            2511..4493
                     /locus_tag="VBC74_001626"
     CDS             2511..4493
                     /locus_tag="VBC74_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEO6942561.1"
                     /translation="MKRFRFATVHLVLIGLILFGLLAICVRLFQSYTALLLAIFVALS
                     FVVALLYYQKITYELSEVEQIELLNDQTEVSLKSLLEQMPVGVIQFDLETNDIEWFNP
                     YAELIFTGDNGHFQSATVKDIITSRRNGTAGQSFEYGDNKYSAYLDTETGVFYFFDNF
                     MGNRRNYDSSMLRPVIGIISIDNYDDIMDTMLEADMSKINAFVTSFISDFTQSKNIFY
                     RRVNMDRYYIFTDYSVLNTLIKDKFDILNEFRKRAQENHLSLTLSMGISYGDGNHNQI
                     GQIALENLNTALVRGGDQIVVRENDSSKKALYFGGGAVSTIKRSRTRTRAMMTAISDR
                     LKVVDSVFIVGHRKLDMDALGASVGMQFFASNIVNASYVVYDPNDMNSDVERAIDYLQ
                     EDGETRLVSVERAFELITQNSLLVMVDHSKTALTLSKEFFNKFADVIVVDHHRRDEDF
                     PKNAVLSFIESGASSASELVTELIQFQQAKDKLSRSQASILMAGIMLDTRNFASNVTS
                     RTFDVASYLRGLGSNSMAIQKISATDFDEYRLINELILKGERIYDNIIVATGEEHKVY
                     SHVIASKAADTMLTMAGIEATFVITKNSSNIGISARSRNNINVQRIMEKLGGGGHFSF
                     AACQIQDKSVKQVRRMLLEIIDEDLRENSTVENRRD"
     gene            4499..4951
                     /gene="rplI"
                     /locus_tag="VBC74_001627"
     CDS             4499..4951
                     /gene="rplI"
                     /locus_tag="VBC74_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEO6942562.1"
                     /translation="MKVIFLQDVKGKGKKGEVKEVPTGYAQNFLLKKNLAKEATTQAI
                     GELKGKQKSEEKAQAEILAQAKELKTQLESETTRVQFIEKVGPDGRTFGSITAKKIAE
                     ELQKQYGIKIDKRHIDLDHTIRAIGKVEVPVKLHKQVSSQIKLDIKEA"
     gene            4994..6349
                     /gene="dnaB"
                     /locus_tag="VBC74_001628"
     CDS             4994..6349
                     /gene="dnaB"
                     /locus_tag="VBC74_001628"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEO6942563.1"
                     /translation="MVEVSELRVQPQDLLAEQAVLGSIFISPEKLIMVREFISPDDFY
                     KYSHKVIFRAMITLADRNDAIDAATVRNILDDQGDLQNIGGLGYIVELVNSVPTSANA
                     EFYAKIVSEKAMLRDIISKLTDTVNMAYEGNDSDEIIATAEKALVDINEHSNRSGFRK
                     ISDVLKVNYENLELRSQQTSDVTGLPTGFRDLDRITTGLHPDQLIILAARPAVGKTAF
                     VLNIAQNVGTKQNRPVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWNNVT
                     IAQGALADAPIYIDDTPGIKITEIRARSRKLSQEVDDGLGLIVIDYLQLISGTRPENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYRREGEEAEEIVEDNTVEVILEKNRAGARGTVKLMFQKEYNKFSSIAQFE
                     E"
     gene            6361..6648
                     /locus_tag="VBC74_001629"
     CDS             6361..6648
                     /locus_tag="VBC74_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEO6942564.1"
                     /translation="MSDAFADVAKMKKIKEDIKSHEGQMVELTLENGRKREKNKIGRL
                     IEVYPSLFIVEYKDTAAVPGAIDNTYVESYTYSDILTEKTLIRYFDDESAE"
BASE COUNT         2232 a          983 c         1405 g         2072 t
ORIGIN      
        1 ggtaggcatt ataggttata aaatgattaa tggtatacat tcaccaacca ataaaaacct
       61 agaggagcat aaggatgatc caatgaatgt ggcagtttca ccattagcaa tgcccctttt
      121 ggcaggtccg ggtactattg caactgctat ggggttatca agtggtggtt tatctggcaa
      181 actcattact atacttgctt ttgccatatt atgtgtgata atgtatgtta ttttaataag
      241 tgcaaacgaa attactaaat ttcttggaaa aaatgcaatg actataatca ccaaaatgat
      301 gggacttatt ttgatgacta tcgggataga gatgctaata acaggcataa aaattggttt
      361 tcatttaaca tagcctatcc cctctggttg acttttaaaa gataaatgct aaaataaatt
      421 taacaataac tatgatggtc aaagctcatg gaatttgtaa gtctttttga cgtacaattt
      481 tcgagagttt tggctatttt tgtgttttaa tgaattagac tttgaaattg gaaaggaata
      541 aaatgacaca taattttgca gaaaactatg atattatcgt tgttggagcg ggtcatgcag
      601 gtgtggaagc tagtctagct gctagtcgta tgggatgtaa gacacttcta gccactatca
      661 acttagaaat gttagcattt atgccttgta atccttctat aggtggctct gctaaaggta
      721 tcgttgtcag agagattgac gctttaggtg gtgaaatggg taaaaacatt gataaaacct
      781 atatacaaat gaaaatgctt aatacaggta aggggcctgc tgtccgtgcc ttaagagcac
      841 aagctgataa agccctctat gcacaaacaa tgaagcaaac tgtagaaaag caagaaaatc
      901 ttaccttaag acaagctatg attgacgaaa tacttgttga agatggaaag gttgtggggg
      961 ttcgtacagc aactaatcag aaattttcag caaaatctgt tgttataaca acaggtactg
     1021 ctcttcgagg agagattata ttaggtgatc ttaagtactc ctcgggccct aataatagtt
     1081 tagcttctgt gacattggca gataatttaa gagacctagg attagaaatt ggtcgtttca
     1141 aaactggaac acctcctcgt gtaaaagcct cctctattaa ttatgaaaag acagagatac
     1201 aacctgggga tgagcaacca aatcattttt catttatgtc tagggatgaa gattatatta
     1261 ctgaccaggt accgtgctgg ttgacctata cgaatacttt gagtcatgac atcatcaatc
     1321 aaaatctaca ccgtgctcct atgttttctg gaatcgttaa gggagttggt cctagatatt
     1381 gtccgtcaat tgaagataaa attgtgcgtt ttgctgataa agagcgccac caactcttct
     1441 tagaaccaga aggtagatac actgaagaag tttatgttca agggctatca acgagtcttc
     1501 ctgaggatgt tcaagttgat ttattacgtt ctataaaagg gcttgaaaat gctgaaatga
     1561 tgcgtacagg ctatgctatt gagtatgata ttgtattgcc tcatcaatta agagcaacac
     1621 tcgaaacaaa agtaattgct ggtttgttta ctgcaggaca gactaatggt acttcaggtt
     1681 atgaagaagc tgctggtcaa gggttggttg ctgggatcaa cgctgctctt aaagttcaag
     1741 ggaagccaga acttatttta aaacgaagtg atgcttacat tggtgtcatg attgatgatt
     1801 tagttaccaa agggactcta gaaccatatc gtctgttgac aagtcgcgca gaatatcgtc
     1861 tgattcttcg tcatgataat gcagatatgc gtttaacaga aatcggttat gagattggcc
     1921 tagtagatga agagcgttat gcgatattta aaaagcgtca aatgcaattt gaaaatgaat
     1981 tagaacgttt agactctata aagttaaagc cagtttctga gacaaataaa cggattcaag
     2041 aattgggctt taaacctttg acagatgctt taactgccaa agaatttatg cgccgcccac
     2101 aaatcacata tgcagtagca acggattttg ttggttgtgc tgatgagcca ttagacagta
     2161 aggttattga acttttggaa actgaaatta aatacgaagg ctatattaaa aaggctttag
     2221 atcaagttgc aaaaatgaaa cgaatggaag aaaaacgtat tccccctcat attgactggg
     2281 acgacattga ttctatcgca acagaagcta gacaaaaatt caaaaaaatt aacccagaaa
     2341 cgctaggtca ggctagtcgt atttcaggag ttaatccagc cgatattagt attttaatgg
     2401 tttaccttga aggaagacaa aaaggtcgta aaaatatcaa ttgataatag tgttcgcccc
     2461 tgtttatcag gggcgttttg tgttataatt aggtcttaag aggttattta atgaaaagat
     2521 ttcgttttgc aaccgtccat ttagttttaa taggcctgat tttatttgga ctattagcta
     2581 tttgtgttag gttatttcaa tcctatacgg ctctattatt ggctattttt gttgctttat
     2641 cttttgttgt tgccttatta tattaccaaa agataactta tgaactatct gaagttgaac
     2701 aaattgaatt acttaatgat caaacagaag tttctcttaa gagtcttctt gaacaaatgc
     2761 cagttggtgt tatacaattt gatcttgaaa caaatgatat tgagtggttt aacccttatg
     2821 cagaattgat ttttactggg gataatggtc attttcagtc tgctactgtt aaagatatca
     2881 ttactagtag acgtaatgga acggctgggc aatcatttga atacggtgat aacaaatatt
     2941 cagcttattt ggatacagag acgggtgttt tctatttctt cgataatttc atggggaata
     3001 gaagaaacta tgatagttca atgctacgtc cagttattgg tattatttcc attgataact
     3061 acgatgatat tatggatact atgttagagg ctgatatgtc aaagattaac gccttcgtga
     3121 ctagttttat ttctgatttt acacagtcta aaaatatttt ttatcgtcgt gtcaatatgg
     3181 atcgatacta tatttttacc gactatagcg ttttaaatac attgataaaa gataaatttg
     3241 atattttaaa tgagtttcga aaacgcgctc aggaaaacca tttatcatta acattaagta
     3301 tgggaatttc ttatggtgat ggtaaccata atcaaattgg gcaaattgct ttagaaaatc
     3361 ttaatacagc actcgttcgc ggaggagatc aaatagtagt acgtgaaaat gatagtagta
     3421 aaaaagcact ttactttggt ggtggtgctg tttcaactat taaacgttca cgaacacgta
     3481 ccagagctat gatgactgct atttctgacc gtttgaaagt agttgatagt gtctttattg
     3541 tgggacacag gaaattggac atggatgcac taggtgcatc tgtagggatg caattttttg
     3601 ctagtaatat tgttaatgcc agttatgtag tttatgaccc aaatgacatg aattctgatg
     3661 ttgaaagagc aattgattac ttgcaagaag acggcgaaac acgtctcgta agtgttgaga
     3721 gagcttttga attaattact caaaattctt tattagtcat ggtagaccat tctaaaacag
     3781 ctttaacctt atctaaggaa ttctttaaca agtttgcaga tgttattgtg gtagatcatc
     3841 atagaagaga tgaagatttt ccaaaaaatg ctgttctttc atttatagaa agtggcgcaa
     3901 gtagtgccag cgaattagtt acagaattga ttcaatttca acaagctaaa gataaattaa
     3961 gccgcagtca agcaagtatt ttaatggctg gtatcatgct tgatacaaga aattttgcat
     4021 ctaatgtaac gagtagaact tttgatgtgg caagctattt acgcggttta ggtagtaata
     4081 gtatggctat tcaaaaaata tcagctacag attttgatga atatcgttta attaatgaac
     4141 tgattttaaa aggtgagcgt atttatgata acatcattgt tgctaccgga gaagaacata
     4201 aagtttactc gcatgttata gctagtaaag cagctgacac catgttgaca atggcaggta
     4261 ttgaggctac ttttgttatt actaaaaaca gttcaaatat tggtatttca gcgcgcagta
     4321 gaaataatat caatgtccag cgtattatgg aaaaacttgg tggtggcgga cattttagtt
     4381 ttgctgcttg tcaaatacaa gacaagtctg taaagcaagt tagacgtatg ttactggaaa
     4441 taattgacga agatttaaga gaaaatagta cagtcgaaaa tagaagggat taattgagat
     4501 gaaagttatt tttttacaag atgttaaggg gaaaggaaaa aaaggtgagg ttaaggaagt
     4561 acctactggt tatgctcaaa acttcttatt aaaaaagaat ctagctaaag aagcaacgac
     4621 acaagctatc ggtgaattaa aaggaaaaca aaagtccgaa gaaaaagcac aagcagaaat
     4681 tttagcacag gcaaaggagc tgaaaacaca attagaatcc gaaactaccc gagtgcaatt
     4741 tattgaaaag gtaggtccag atggtcgtac gtttggttct atcacagcca aaaagattgc
     4801 tgaagagtta caaaaacaat acggcattaa aattgacaaa cgtcatattg atttagatca
     4861 tactattaga gcgattggta aggttgaagt accagtaaaa ttacataaac aggtttcttc
     4921 acagattaag cttgatatca aagaagcgta aactgagaga tgataaaggt tgacaagtca
     4981 ggaggaatga agtttggtag aagtatctga gctaagagta caacctcaag acctgttggc
     5041 agaacaggcc gttctaggat ctatctttat ctcaccagag aaactgatta tggtgagaga
     5101 atttataagt ccagatgatt tttataaata ttcacacaaa gttatcttca gagcgatgat
     5161 taccttagct gatagaaatg atgcaattga tgctgctact gttagaaata ttttagatga
     5221 ccaaggtgat cttcaaaaca ttggtgggtt gggctatatt gttgaattag ttaatagtgt
     5281 tccaacaagt gctaatgcgg agttttatgc caaaatagtt tctgagaaag ccatgttgcg
     5341 agatatcatt tctaagttga cagatactgt caacatggcc tatgaaggaa atgattctga
     5401 tgaaattatt gccaccgctg agaaggcttt agttgatatt aacgaacaca gtaatcgtag
     5461 cgggtttaga aagatatctg atgttttaaa agttaattac gaaaacttag aattacgttc
     5521 acagcaaacc tcagatgtca caggtttacc aactggattt agagatttag acaggattac
     5581 tacaggttta catccagatc aattaatcat cttagcggct cgtcctgcag taggtaaaac
     5641 agcctttgtt ttaaatattg ctcaaaatgt tggaactaaa caaaatagac ctgtagctat
     5701 tttttcactt gaaatggggg ctgagagttt agttgaccgt atgttggcag cagagggaat
     5761 ggttgattct catagtttac gaactggtca gttgacggat caagattgga ataatgtaac
     5821 gattgctcaa ggagctttgg cggatgcgcc aatttatatt gatgatacgc ccgggattaa
     5881 aattacagaa attagagcac gctctaggaa gttatctcaa gaagttgatg atggtttagg
     5941 tttaatcgtc attgactacc tccaattaat ttcaggaact agaccagaaa atcgtcagca
     6001 agaagtttct gaaatttcaa ggcagttgaa gattttagct aaagaattaa aggttcctgt
     6061 tatcgcatta agccagctct ctcgtggggt tgagcaaagg caggataaac gaccggtctt
     6121 atctgatatt cgtgaatctg gatcgattga acaagatgct gacattgttg ctttcttata
     6181 tcgagatgat tattatcgta gagagggcga agaagcagag gaaattgtag aagataatac
     6241 tgttgaggtt attttagaaa agaaccgtgc tggtgcgcgt gggacagtaa aattaatgtt
     6301 tcaaaaggaa tataataaat tttctagtat agctcaattt gaagaatagg agatgttaat
     6361 atgagtgatg catttgcaga tgttgcaaaa atgaaaaaaa taaaagaaga tatcaagtca
     6421 catgagggac agatggtcga acttactctc gaaaatggtc gtaagaggga aaaaaataaa
     6481 attgggcgcc taattgaagt ttacccatct ttgtttatcg tagagtataa ggatactgca
     6541 gctgtaccag gagcaattga taatacttat gtagagtctt acacttattc agatatttta
     6601 actgagaaaa ctttaattcg ttattttgat gacgagtcag cagaatagta tgaaaacctc
     6661 gactgaggtt ttttgtttct ataaaaaaat tt
//
LOCUS       DATROD010000091         6587 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.093, whole genome shotgun sequence.
ACCESSION   DATROD010000091 DATROD010000000
VERSION     DATROD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.093"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..545)
                     /locus_tag="VBC74_001630"
     CDS             complement(<1..545)
                     /locus_tag="VBC74_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO6942565.1"
                     /translation="MTESVTVNKKRIKQITQTQEDILTAFGQLLEEYPYEKIQISQIA
                     KKSGYARRTFYRHFDSRDDLLTLFIERLTLNLFKQLGQLEQPTFSQVFQNFFSYWSDY
                     KSLLLILRKNDLLPQFQQSWFRHIDLIELGRGDLSSNTYAQRFAIGGIFSVLIEWIHQ
                     DCQTSIEELTQVSFDIINHLK"
     gene            637..1286
                     /locus_tag="VBC74_001631"
                     /pseudo
     CDS             637..1286
                     /locus_tag="VBC74_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645479.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
     gene            1298..1948
                     /locus_tag="VBC74_001632"
     CDS             1298..1948
                     /locus_tag="VBC74_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HEO6942566.1"
                     /translation="MSYFRNYWYRFGAILFIILAVILLVFRPDWSMLHYLLYFYFMAL
                     LAHQFEEYQFPGGASPIINYVVYDEEELMDCFPGNTQSIMLVNTIAWLLYIASIAFPQ
                     AYWLGLGVMFFSLTQLLGHGFQMNIKLKTWYNPGLATTVFLLVPIACAYIYQASAEGM
                     LTWGDWLGGFIMLIVCVLTSIIAPVQLLKDKETNYIISPWQMDRFHKVVNFVRIKK"
     gene            2078..3454
                     /locus_tag="VBC74_001633"
     CDS             2078..3454
                     /locus_tag="VBC74_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEO6942567.1"
                     /translation="MENHRYEDEGKFQRKMTSRHLFMLSLGGVIGTGLFLSSGYTIAQ
                     AGPLGAVLSYLIGAVVVYLVMLSLGELAVAMPVTGSFHTYATKFISPGTGFTVAWLYW
                     ICWTVALGTEFLGAAMLMQRWFPNVPAWAFASFFALVIFGLNALSVRFFAEAESFFSS
                     IKVIAIIIFIILGLGAMFGLVSFEGQHKAILFTHLTANGAFPNGIVAVVSVMLAVNYA
                     FSGTELIGIAAGETDNPKEAVPRAIKTTIGRLVVFFVLTIVVLASLLPMKEAGVSTAP
                     FVDVFDKMGIPFTADIMNFVILTAILSAGNSGLYASSRMLWSLANEGMLSKSVVKINK
                     HGVPMRALLLSMAGAVLSLFSSIYAADTVYLALVSIAGFAVVVVWLAIPVAQINFRKE
                     FLKENQLEDLSYKTPFTPVLPYITIILLLISIVGIAWDSSQRAGLYFGVPFIIFCYIY
                     HKLRYKKW"
     gene            3459..4403
                     /gene="mmuM"
                     /locus_tag="VBC74_001634"
     CDS             3459..4403
                     /gene="mmuM"
                     /locus_tag="VBC74_001634"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HEO6942568.1"
                     /translation="MGRFKELLESKKTLILHGALGTELESRGCDVSGKLWSAKYLIED
                     PAAIQTIHEDYIRAGADIVTTSTYQATLQGLAQVGVSESQAEDLIRLTVQLAKAAREQ
                     VWKSLTKEEKSERIYPLISGDVGPYAAFLADGSEYTGLYDIDKQGLKNFHRHRIELLL
                     DEGVDILALETIPNAQEAEALIELLAEDFPQVEAYMSFTSQDGKTISDGSAVADLAKA
                     IDVSPQVVALGINCSSPSLVADFLQAIAEQTNKPLVTYPNSGEVYDGASQSWQSSPDH
                     SHTLLENTSDWQKLGAQVVGGCCRTRPADIADLSAHLK"
     gene            complement(4496..6562)
                     /locus_tag="VBC74_001635"
     CDS             complement(4496..6562)
                     /locus_tag="VBC74_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO6942569.1"
                     /translation="MDDIFNSLMGNMGGYNSENKRYLINGREVTPEEFSQYRQTGKLP
                     GQELNNQNTPTNQVSADSVLTKLGTNLTDQARQHLLDPVIGRNKEIQETAEILARRTK
                     NNPVLVGDAGVGKTAVIEGLAQAIINGDVPAAIKNKEIISIDISSLEAGTQYRGSFEE
                     NIQNIIKEVKETGNIILFFDEIHQILGAGSTGGDSGSKGLADILKPALSRGELTVIGA
                     TTQDEYRNTILKNAALARRFNEVKVNAPSAQDTFNILMGIRNLYEQHHNVVLPDSVLK
                     AAVDLSIQYIPQRSLPDKAIDLIDMTAAHLAAQHPVTDLKSLEKEIAEQRDKQEKAVN
                     TEDFEEALKVKTRIEELQNQIDNHTEGQKVTATINDIAMSIERLTGVPVSNMGASDIE
                     RLKELGNRLKGKVIGQNDAVEAVARAIRRNRAGFDDGNRPIGSFLFVGPTGVGKTELA
                     KQLAFDMFGSKDAIVRLDMSEYNDRTAVSKLIGATAGYVGYDDNSNTLTERIRRNPYS
                     IVLLDEIEKADPQVITLLLQVLDDGRLTDGQGNTINFKNTVIIATSNAGFGNEAFTGD
                     SDKDLKIMERISPYFRPEFLNRFNGVIEFSHLSKDDLSEIVDLMLDEVNQTIGKKGID
                     LVVDENVKSHLIELGYDEAMGVRPLRRVIEQEIRDRITDYYLDHTDVKHLKANLQDGQ
                     IVISER"
BASE COUNT         1739 a         1256 c         1333 g         2259 t
ORIGIN      
        1 ttttttaaat gattgataat atcgaaggaa acttgagtta attcttcgat acttgtttgg
       61 cagtcctgat gaatccactc aattagaaca ctgaaaatac caccaatcgc aaatcgctga
      121 gcataggtat tggaagataa atcaccacga cctaactcta tcaaatcaat atgtcggaac
      181 caggattgtt gaaattgagg gagtaggtca ttctttctaa gaatgagaag aagggattta
      241 taatcagacc aataactaaa aaaattttgg aaaacttggc taaaagtagg ttgctccaat
      301 tgaccaagtt gcttaaagag atttaatgtt agtctttcga tgaaaagggt aagtaggtca
      361 tctcgactgt caaagtgtcg gtaaaaggtc cttctagcat agccagattt cttggcaatt
      421 tggctaatct gaattttctc atagggatac tcttccaaaa gttggccaaa agctgttaag
      481 atatcctctt gtgtttgagt gatttgtttt attctctttt tattaactgt cacactttca
      541 gtcatttgtg cataaaacct ccaaaggtat tgatattata ggttcacttg attatactat
      601 atggtatcaa atgtcacaaa tcttggaggt catcttatga aagcattgat aacaaaatgg
      661 tacctctttt gtccctatct tgcttctctt tttgccttag cattattttt tggaaattgg
      721 gatttacgag tgcagtcact acttatttcg ggacttttta ttcaattaca ctttttcgaa
      781 gaatttggtt tccctggagg ttttccgctt atcactatgc tagttgaact taaatcggtt
      841 gaaactgata ctagtaagtg ggacttaaac catctatcag cctttttcgg gaatcagtgg
      901 tttgcagtga ttgtttatct tttgccgatt ttttgtccca atataccatt tttaacactt
      961 gcagtcatga tttttgcatt tgcagaactt gccatgcatc ttttcttttt taatctttcc
     1021 ttgaagaagt ggtataatcc aggacttctt acgaccttag taggtcttgt ccctgtttct
     1081 gtctattatt tagctcatga ttggaagttg tattctggac ttgattggtt tttaggctga
     1141 tatggattgt tcttaactac tttatagcct ttcgttcccc aatctataag cgtttaggtc
     1201 gacattcaaa ttacgcattt agtgatgttg atctttcacg ctcaaaacca tttttaacac
     1261 attttcgtga aacacagttt aaataggagg tattatcatg tcatatttta gaaattactg
     1321 gtatcgtttt ggagcaattt tatttattat tttagcagta atattgcttg tttttagacc
     1381 tgactggtca atgcttcact atctattgta tttttacttt atggcacttc tagcgcatca
     1441 atttgaagaa tatcagtttc ccggtggggc atcacctatc attaactatg ttgtttatga
     1501 tgaagaagag ctgatggatt gttttccagg caatactcag tctattatgt tggttaatac
     1561 tattgcttgg ttgctttaca ttgctagtat tgcttttcct caagcttatt ggcttggatt
     1621 aggagtcatg ttctttagtc taacgcagct cttgggtcat ggttttcaga tgaatattaa
     1681 acttaaaact tggtataatc ctggtctagc aacgacagta tttctcctag taccaatagc
     1741 ttgcgcatac atctatcaag ctagtgcaga aggaatgctc acttggggag attggctagg
     1801 tggttttatc atgttgattg tctgtgtact aactagcatt attgcacctg tacagctatt
     1861 gaaggataag gagaccaatt atattattag tccttggcaa atggaccgtt ttcataaggt
     1921 cgttaatttt gtaaggataa aaaaataagg ttaccgctat ttatgattcg ctgatttttt
     1981 gtaatagttt ttctttatca tatatgatag ctaataccta ttccccaata cgactagatt
     2041 gtggtaggat aaaagtatta gtttcttgga ggttgttatg gaaaatcatc gttatgaaga
     2101 tgaaggtaaa ttccagcgta agatgaccag tcgtcatctc tttatgttat cgctaggtgg
     2161 tgttatcggg actgggcttt tcttgagttc aggttatacc attgcacagg ctggtccgct
     2221 tggagctgtg ctgtcttatt tgattggtgc cgttgtggtt tatttggtca tgctatcact
     2281 tggggaattg gcggttgcca tgccggtgac ggggtcattc cacacttatg ccactaagtt
     2341 tatcagtcct ggaacaggtt ttactgttgc ttggctatat tggatttgtt ggacggtcgc
     2401 cttggggact gaatttttag gtgctgccat gctgatgcag cgctggttcc caaatgtgcc
     2461 ggcttgggca tttgcttcct tttttgccct tgtgattttt ggtttaaatg ctcttagcgt
     2521 acgctttttt gcagaagcag agtctttctt ctcaagtatt aaggttattg ctatcattat
     2581 ctttattatc ttgggcttag gtgctatgtt tggtctagtt tcctttgaag gtcagcacaa
     2641 ggctattctc ttcactcatc tgactgccaa tggtgccttt ccaaatggta tcgttgcagt
     2701 tgtctcagtc atgttggctg ttaactatgc cttctctggt actgagttaa ttggtattgc
     2761 ggctggtgaa acggataatc ccaaagaagc tgtaccaagg gctattaaaa cgacaatcgg
     2821 tcgcttggtt gttttctttg tactgacaat tgttgtccta gcttcgctat tgccaatgaa
     2881 agaggcaggc gtatccacag caccattcgt tgatgtcttt gacaagatgg gaatcccttt
     2941 tacggcggat atcatgaact tcgttatctt gacagccatc ctgtctgctg gtaactcagg
     3001 tctctacgca tcaagccgta tgctctggtc ccttgccaat gaaggtatgt tgtcaaaatc
     3061 tgttgtgaaa atcaataaac acggtgtccc aatgcgtgct cttctcttgt caatggcagg
     3121 agcagtgctg tcgctctttt caagtattta cgctgcagac acagtttatc tagccttggt
     3181 ttcaatcgcg ggctttgctg ttgttgtcgt atggctagcc attccagtcg cacaaatcaa
     3241 tttccgcaag gaattcttga aagagaatca actagaagat ttgtcttaca aaacaccttt
     3301 tacaccagtt ctcccttata tcacaatcat tttgctcttg atttcgattg tagggattgc
     3361 ttgggatagc tcacaacgtg ctgggcttta cttcggtgtt ccatttatta ttttctgcta
     3421 tatttatcac aaactacgtt acaagaaatg gtgatcttat gggaagattt aaagaacttt
     3481 tagagagtaa aaaaactctc atcctacatg gtgccttggg gaccgagctt gagagtagag
     3541 gttgtgatgt ttctgggaaa ctttggtcag ccaaatatct gattgaggat ccagcagcta
     3601 ttcagaccat tcatgaagat tatattcgtg ctggcgctga tattgtgacg acatcaactt
     3661 atcaggcgac tttacaagga ctagctcaag ttggtgtctc tgaaagtcag gcagaagatt
     3721 tgattcgctt gactgttcag ttggcaaaag ctgcgcgtga gcaagtttgg aaaagtttga
     3781 caaaagaaga aaaatcagag agaatctatc ctttgatttc tggggatgtt ggtccctacg
     3841 ctgccttttt agcggatggc tctgagtaca ctggactcta cgatattgat aagcaaggtc
     3901 ttaaaaactt ccatcgccat cgtattgagc ttcttttaga tgagggtgtt gacattctgg
     3961 cacttgaaac cattccaaac gctcaggagg ctgaggctct tattgagcta cttgccgagg
     4021 atttccctca ggttgaggct tatatgtcat tcacctctca agatggtaag accatctcag
     4081 atggtagtgc tgttgcagac ttggcgaaag ctattgatgt tagcccacag gtcgtggcgc
     4141 tgggcattaa ctgttcaagt ccatcgttag ttgctgattt cttgcaagca atagcagagc
     4201 agactaacaa gcctcttgtg acctatccaa actctggaga ggtttatgat ggggcaagcc
     4261 aatcttggca atctagccct gatcattcac acaccctact tgaaaataca agtgactggc
     4321 aaaaacttgg cgcccaagtt gtcggaggtt gttgccgaac tagaccagct gatattgctg
     4381 acctctctgc acatttaaaa taagtaaagt atcttgaaga aggttagggt aagtttgatt
     4441 tcctttttca atccactaga aaacccgctt ggattcttcc aaacgggttt tgtttttatc
     4501 tttcagaaat gacgatttgg ccatcttgca aattagcttt taggtgttta acgtctgtat
     4561 gatcgagata gtagtctgtg atgcgatctc gaatttcttg ctcgatgaca cggcgcaatg
     4621 gacgtactcc cattgcttcg tcataaccca gttcaattaa gtgtgattta acattttcat
     4681 ctaccacaag gtcaattcct tttttgccaa ttgtttggtt aacttcatca agcatcaaat
     4741 ctacaatttc gcttaagtca tctttgctta ggtgagagaa ttcaataaca ccattgaaac
     4801 gatttagaaa ttctggacgg aaatatggag aaattcgttc cataattttc aagtctttat
     4861 cgctgtcacc tgtaaatgct tcgtttccaa aacctgcatt tgaggttgca atgataacag
     4921 tatttttaaa gttaatagtg tttccttgac catcagttaa acggccatca tctagaactt
     4981 gaaggagaag cgtgataact tgcgggtcag ctttttcgat ttcatcaaga agaacaatag
     5041 aataaggatt tcgacggata cgttctgtta gtgtattact attgtcgtca taaccaacgt
     5101 aaccagcagt cgctccaatt agcttagaaa ctgctgtacg atcattatat tcagacatat
     5161 caagtctaac gatagcatcc tttgatccga acatatcaaa agctaattgc tttgctagct
     5221 cggttttacc aactccagtt ggtccaacaa atagaaagct accaatcgga cgattaccat
     5281 catcgaaacc agcacgattt cggcgaatag ctctcgcaac tgcctcaaca gcatcatttt
     5341 gaccaatgac tttacctttt agacgattgc caagttcttt taagcgttca atatcactag
     5401 cacccatatt tgatacagga actccagtta gacgctcaat tgacatagcg atatcgttga
     5461 tagttgcagt gactttttgt ccttcggtat gattatcaat ctggttttgt aactcttcaa
     5521 tgcgtgtctt gaccttaaga gcttcttcaa aatcttcagt attgacagct ttttcttgct
     5581 tgtctctttg ctcagcgatt tctttttcta aggatttaag gtcagttacg ggatgttgcg
     5641 cagctaggtg tgcagcagtc atatcaataa ggtcaattgc tttatcaggg agacttcttt
     5701 gtgggatgta ttgaatagat aaatctacag ctgctttcaa aacagagtca ggtaaaacaa
     5761 cattatgatg ttgctcatag aggtttctta tccccataag gatattgaaa gtatcttgtg
     5821 cagaaggtgc attgacttta acttcgttaa agcgacgtgc taaggcagca ttcttgagaa
     5881 tggtgttacg atattcatct tgagtagttg cacctataac cgttaactca ccacgtgaaa
     5941 gtgcaggctt aagtatatct gcgagcccct tagagccact atcaccacca gttgaaccgg
     6001 ctcctaatat ttggtgtatt tcatcaaaga agagaataat atttcctgtt tccttaactt
     6061 cttttatgat attttgaata ttttcttcaa aactacccct atattgggtg ccagcttcaa
     6121 ggctagaaat atctatcgaa atgatttctt tatttttgat agcagcaggg acatcaccat
     6181 ttataattgc ttgtgccaaa ccttcaataa cagctgtttt accaacacct gcatcaccaa
     6241 ccagaacagg attattttta gtacgacgag ctagaatttc tgcagtttcc tgaatttctt
     6301 tgtttcgtcc aataactgga tctagcaaat gctgacgtgc ttgatcggtt aaattagttc
     6361 ctaatttagt caaaacacta tctgcagaga cttggtttgt aggagtgttt tggttattta
     6421 gctcttgacc ggggagtttt ccagtttgac gatattgact gaattcttca ggagtaactt
     6481 cccgtccatt gattaagtat cgcttattct cactgttata gccccccata tttcccatta
     6541 gagaattaaa aatatcatcc atatttccaa aagggtcacg attataa
//
LOCUS       DATROD010000092         6547 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.094, whole genome shotgun sequence.
ACCESSION   DATROD010000092 DATROD010000000
VERSION     DATROD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.094"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            56..826
                     /gene="sufC"
                     /locus_tag="VBC74_001636"
     CDS             56..826
                     /gene="sufC"
                     /locus_tag="VBC74_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEO6942570.1"
                     /translation="MSVLEIKNLHVSIEDKEILKGLNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTAGEILFDGEDILELEVDERARLGLFLAMQYPSEVPGITNAEFIR
                     AAMNAGKADDDKISIRQFITKLDEKMELLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGEGFGAMIITHYQRLLNYITPDKVHV
                     MMDGKVVLSGGPELAVRLEKEGYAQIAEELGLEYKEEV"
     gene            863..2125
                     /gene="sufD"
                     /locus_tag="VBC74_001637"
     CDS             863..2125
                     /gene="sufD"
                     /locus_tag="VBC74_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEO6942571.1"
                     /translation="MSKEAILNFLQAKGEPTWLQELRLKAFEKIEELELPVIERVKFH
                     RWNLGDGTILENDYTANIPDFTELGNNPKLVQIGTQTVLEQVPMELIEKGVVFTDFYS
                     ALEEIPEVIERYFGKARPFEEDRLAAYHTAYFNSGAVLYIPDNVEITQPIEGLFYQDS
                     QSKVPFNKHILLIVGKNAKVSYLERFESIGDGTERTSANISVEVIAQAGSQIKFASID
                     RLGENVTTFISRRGRHSSDATIDWALGVMNEGNVVADFDSDLIGDGSHANLKVVAASS
                     GRQVQGIDTRVTNYGCNSVGHILQHGVILERGTLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDKARSDANPILLIDENDVTAGHAASIGQVDPEDLYYLMSRGLNQKTAEQLVIRGF
                     LGTVIAEIPVKEVRDEMIAVIDTKLEKR"
     gene            2127..3359
                     /locus_tag="VBC74_001638"
     CDS             2127..3359
                     /locus_tag="VBC74_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEO6942572.1"
                     /translation="MTLLDSYKLKQDFPILNQLVNDEPLIYLDNAATTQKPNQVLETL
                     RDYYQNDNANVHRGVHTLAERATAQYETAREKARQFLNAKSSKEILFTRGTTTGLNWV
                     AKFAESILERGDEVLISIMEHHSNIIPWQQACERTGAKLVYAYLKDGSLDLEDFYNKL
                     SSKTKFVSLAHISNVLGCITPVKAIAERVHQVGAYMVVDGAQSAPHMAIDVQDLDCDF
                     FALSGHKMLGPTGIGVLYGKESILDKMPPVEFGGEMIDFVYEHSATWKELPWKFEAGT
                     PNIAGAIAFGEALDYLTDVGMDEIHQYEQSLVSYVLPKLQAIDGLTIYGPSDAESHVG
                     VIAFNLEGLHPHDVATAMDYEGVAVRAGHHCAQPLINHLGIHSAVRASFYFYNTKEDC
                     DKLVDAIQKTKEFFNGTL"
     gene            3346..3789
                     /locus_tag="VBC74_001639"
     CDS             3346..3789
                     /locus_tag="VBC74_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEO6942573.1"
                     /translation="MALSKLDNLYMAVVADHSKHPHHHGFLEGVEQVQLNNPTCGDVI
                     SLSVKFDGNIISDIAFAGNGCTISTASSSMMTDAVIGKTKEEALQLADVFSKMVQGNQ
                     NPKQEKLGDAEFLAGVSKFPQRIKCATLSWNALRKAIERDNQAEL"
     gene            3889..5307
                     /gene="sufB"
                     /locus_tag="VBC74_001640"
     CDS             3889..5307
                     /gene="sufB"
                     /locus_tag="VBC74_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEO6942574.1"
                     /translation="MSEINEKVEPQPIDLGEYKFGFHDDVKPIYSTGKGLNEAVIREL
                     SAAKGEPEWMLDFRLKSLETFNKMPMQTWGADLSDIDFDDIIYYQKASDKPARDWDDV
                     PEKIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEYDKLGIVFTDTDSALKEY
                     PELFKKYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRATAKKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANKGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKDSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSEAEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(5378..6535)
                     /locus_tag="VBC74_001641"
     CDS             complement(5378..6535)
                     /locus_tag="VBC74_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO6942575.1"
                     /translation="MLLSLTCVNSVQAEEHKDIMQITREAGYDVKDINKPKASIVIDN
                     KGHILWEDNADLERDPASMSKMFTLYLLFEDLAKGKTNLNTTVTATETDQAISKIYEI
                     SNNNIHAGVAYPIRELITMTAVPSSNVATIMIANHLSQNNPDAFIKRINETAKKLGMT
                     KTHFYNPSGAVASAFNGLYSPKEYDNNATNVTTARDLSILTYHFLKKYPDILNYTKYP
                     EVKAMVGTPYEETFTTYNYSTPGAKFGLEGVDGLKTGSSPSAAFNALVTAKRQNTRLI
                     TVVLGVGDWSDQDGEYYRHPFVNALVEKGFKDAKNISSKTPVLKAVKPKKEVTKTKTK
                     SIQEQPQTKEQWWTKTDQFIQSHFVSILIVLGTIASLCLLAGIVLLIKRSR"
BASE COUNT         2096 a         1049 c         1395 g         2007 t
ORIGIN      
        1 ttaaaccata gagaaatatc acatattctc tgttaaccaa gaaaaggagt gtacaatgtc
       61 tgtacttgaa atcaaaaatc ttcatgtttc tattgaagat aaagaaattt taaaaggtct
      121 taatttaacg ctgaaaacag gagaaattgc tgctatcatg ggtccaaatg gtactgggaa
      181 gtcgacactt tcagcagcta ttatgggaaa tcctaattat gaagttacag caggtgaaat
      241 tctttttgat ggtgaagata ttttagagct agaagtggat gaacgtgctc gtttgggtct
      301 atttcttgcg atgcaatacc cttcagaggt tccagggatt actaatgctg aatttatccg
      361 agcagctatg aatgcgggaa aggctgatga tgataaaatt tccatccgcc aatttattac
      421 taagttagat gaaaagatgg aactacttgg catgaaagaa gaaatggcgg aacgttatct
      481 taacgaaggt ttttcaggtg gtgagaagaa gcgcaatgaa attttacaac tcctaatgtt
      541 agagcctaag tttgccctct tggatgagat tgactctggc ctggatattg atgctcttaa
      601 agtggtatca aagggagtca atgaaatgcg tggtgaaggt tttggtgcta tgattattac
      661 acactatcaa cgccttttga attacattac accagataaa gtacacgtta tgatggacgg
      721 gaaggtagta ttgtcaggag gaccagaatt agcggttcgc cttgagaaag aaggttatgc
      781 tcaaatcgct gaagagcttg gtttagagta taaagaagaa gtctaatttg tcacttcaac
      841 tagaagagaa aaggagttac aaatgtctaa agaagctata ttaaattttt tacaagccaa
      901 aggtgagcct acttggttac aagaattacg actaaaggct tttgaaaaaa tagaagaact
      961 agaattacca gtaattgaac gtgtaaaatt tcatagatgg aatttaggtg atggaactat
     1021 tttagagaat gactatacag ctaacattcc agatttcaca gagttaggaa acaatcccaa
     1081 gttggttcaa attgggaccc aaactgtcct tgaacaagtt cctatggaat taatagagaa
     1141 aggcgttgtc tttacagatt tctattcggc tttggaagaa attccagaag tcatagagcg
     1201 ctactttggg aaagcacgtc cttttgaaga agatagactt gctgcctatc acacagctta
     1261 cttcaacagt ggtgcagtgc tttatattcc agataatgtt gaaattactc aaccaatcga
     1321 aggtcttttt tatcaagata gtcaatctaa agtaccattt aataagcata tccttcttat
     1381 tgttggaaaa aatgcaaaag ttagttactt agagcgattt gagtcaattg gtgatgggac
     1441 agaacgaaca agtgctaata tttctgtaga agtaattgcc caagcaggta gccaaattaa
     1501 atttgcctca attgatcgcc tcggagaaaa tgtgacaacg tttataagtc gtcgtggtcg
     1561 tcacagtagc gatgcaacaa tagattgggc ccttggggtg atgaatgagg gcaatgttgt
     1621 tgctgacttt gattctgatt taattggtga tggaagccac gctaatttaa aagtagttgc
     1681 tgcttcgtca gggcgtcaag ttcaaggaat tgatacgcgt gttacaaact acggctgtaa
     1741 ttcagtagga catattttac agcatggggt tattttagag cgtgggactc ttacttttaa
     1801 cggtatcgga catattataa aaggtgcaaa gggagcagac gcccaacaag aaagccgagt
     1861 tctcatgtta tcagacaaag cacgttcaga tgctaatcct atccttttga ttgatgaaaa
     1921 tgatgttaca gctggtcacg cagcctcaat tggtcaggtt gacccagagg atttatatta
     1981 tttgatgagc cgtggcttga accaaaaaac agcagagcaa ttagttattc gtggattttt
     2041 aggaacagtc attgctgaaa taccagtaaa agaagttcgc gatgaaatga ttgccgttat
     2101 tgatacaaaa ctagaaaaac gttaatatga cattactaga tagttataaa ctaaagcaag
     2161 atttcccaat tttaaatcaa ttagttaatg atgagccgct tatctattta gataatgcag
     2221 caacgacaca aaaaccgaat caagttttag aaactttgcg agattactac caaaatgaca
     2281 atgcaaatgt ccatcgtggt gttcatactt tagcagaacg agcgactgct cagtacgaga
     2341 ctgctcgtga aaaagcaagg cagtttttaa atgctaaatc aagtaaagaa attttattca
     2401 cacgtggcac cacaacagga ttaaattggg tagctaagtt tgccgaaagt attttagaac
     2461 gaggtgatga ggtacttatt tccattatgg agcatcattc taatattatc ccatggcaac
     2521 aagcttgcga gagaactggt gctaaattgg tttatgccta tctaaaagat ggttctttag
     2581 atttagaaga tttctataat aaattatcct ctaaaactaa atttgttagt ttggcacata
     2641 tctcaaatgt acttggctgt attacaccag taaaagcaat agcagaacgt gtgcatcaag
     2701 ttggggcata catggttgtt gatggagctc aatcagcgcc acatatggcg attgatgttc
     2761 aagaccttga ttgtgatttc ttcgccttat ctggccataa gatgcttggt cctactggta
     2821 ttggtgttct ttatgggaaa gaaagcattt tagataagat gccacctgtc gaatttggtg
     2881 gtgaaatgat tgattttgtc tatgagcata gtgcaacatg gaaggaattg ccctggaagt
     2941 ttgaagctgg aacgcctaat attgctggag cgatcgcttt tggagaagct ttagattatt
     3001 taacggatgt tggtatggat gagattcatc aatatgaaca aagtttagtt tcttatgtgt
     3061 tgcctaaatt acaggctatc gatggtttaa ctatttacgg acctagtgat gctgaatctc
     3121 atgtaggcgt tattgccttc aatttagaag gtttacaccc acatgatgtg gcaactgcaa
     3181 tggattacga aggagtggct gttcgtgcag gtcatcattg tgcacaaccc cttattaatc
     3241 atcttggtat ccattcggcg gttcgagcaa gtttctattt ctataatacc aaagaggatt
     3301 gcgacaaatt ggtagacgct atccaaaaaa ctaaggagtt tttcaatggc actctctaaa
     3361 ttagataacc tctacatggc ggtggtagct gatcattcaa aacaccctca tcatcatgga
     3421 tttttagaag gcgttgaaca agtgcagtta aacaatccta catgtggaga tgttatttcc
     3481 ttatctgtta aatttgatgg gaatattatt tcagatattg catttgctgg taatggttgt
     3541 acaatttcta cggcttcatc aagcatgatg acagatgcag ttattgggaa aacaaaagaa
     3601 gaagcattgc agcttgctga tgtattctcc aaaatggttc aaggcaatca aaatccaaaa
     3661 caagagaaac ttggggatgc agagtttcta gctggggtat caaaatttcc acaacgtatc
     3721 aaatgtgcta cgctttcgtg gaatgcttta agaaaggcta ttgagagaga taatcaggca
     3781 gaactctgac aaccatagtt gaaggaatta catttaaaca acttttactg acataaggta
     3841 gtctagtaga agaatgtctt aaatttaaat attaaagaaa ggataagtat gtccgaaata
     3901 aatgaaaaag tagaaccaca gccaatagat cttggggaat ataaatttgg atttcatgac
     3961 gatgtaaagc caatttattc tacgggaaaa ggtctaaatg aggctgttat tcgtgagtta
     4021 tctgcagcta agggtgaacc tgagtggatg ttggactttc gtctaaaatc cttggaaacg
     4081 tttaataaaa tgccgatgca gacctgggga gcagatttat cagatattga ttttgatgat
     4141 attatttatt atcaaaaagc atctgataaa cctgcgcgtg attgggatga tgttccagaa
     4201 aaaatcaaag aaacttttga aagaattggg attccagaag ctgaaagagc ctatcttgca
     4261 ggagcatcag cacaatatga atcagaagta gtttatcaca atatgaaaga agaatatgat
     4321 aagctgggta ttgtttttac ggatactgac tctgcactta aagagtaccc agagctattc
     4381 aaaaaatatt ttgctaaact tgtccctcca acagataata aattagctgc tctgaactct
     4441 gctgtatggt caggtggaac atttatttat gttcctaaag gtgttaaggt ggatattcca
     4501 cttcaaactt acttccgtat taataatgaa aatactggac aatttgaacg tactctcatt
     4561 attgttgatg agggagcaag tgttcactat gttgaaggtt gtaccgcccc aacttattct
     4621 tcaaatagtt tacatgcagc tatagttgaa atttttgcac ttgatggagc ttatatgcgc
     4681 tatacgacta ttcaaaattg gtccgataat gtctataatt tagtgacaaa acgtgctacc
     4741 gctaaaaaag atgcaacagt tgagtggata gatggaaatc taggagctaa aacaacaatg
     4801 aaatacccat cggtttacct tgatggtgaa ggagcacgtg gcacgatgtt gtctattgct
     4861 tttgcaaaca aaggacaaca ccaagatacg ggtgcaaaga tgattcataa tgccccccat
     4921 actagttcat ccattgtctc taaatcaatt gctaagggtg ggggaaaagt tgattatcga
     4981 ggtcaagtga catttaataa agattccaaa aaatcagtgt cacatataga atgtgacacc
     5041 atattgatgg atgatatttc aaaatcagat accataccgt ttaatgagat tcataattca
     5101 caggttgctt tagagcatga agcaaaggtg tctaagattt ctgaagagca actgtactac
     5161 ttgatgagtc gaggtttatc tgaagctgaa gcaacagaaa tgattgttat ggggtttgtt
     5221 gagcccttta cgaaagaatt accaatggaa tatgcggtag agttaaatcg tttaatttcc
     5281 tatgaaatgg aaggttcagt tggttaattg aagatttcaa gagataataa aaagccccta
     5341 tgttattgat gaaggggctt ttatattagc acagttatta tctagagcgc tttataagta
     5401 atactatccc agctaaaaga caaaggctag cgatggtgcc cagaacaatt aaaatagata
     5461 caaaatgtga ttggataaat tgatctgttt ttgtccacca ctgttctttt gtttgaggct
     5521 gttcttgaat agatttagtt ttggttttag taacttcttt tttaggttta acggctttta
     5581 atacaggagt tttagaagaa atatttttag cgtctttaaa acctttttct acaagagcgt
     5641 tgacaaacgg atgacgatag tactctccgt cttggtctga ccaatcgcca actcctaaaa
     5701 ccacagttat caagcgagta ttctggcgtt tagctgtaac taaggcatta aaagcagcgc
     5761 tagggctaga accagttttt aagccatcta ctccttctaa tccaaattta gcgccggggg
     5821 tagagtagtt ataagttgta aatgtttctt cataaggagt tccgaccatg gccttgactt
     5881 caggatattt tgtatagttc agtatatcag ggtatttttt aaggaaatga taggttaaaa
     5941 ttgatagatc acgtgcagtc gtaacgttag tagcattgtt atcgtattct tttggggagt
     6001 aaagtccatt aaaagcactc gctaccgccc cactggggtt ataaaagtga gtttttgtca
     6061 taccgagttt cttggcggtt tcattgattc gtttaataaa ggcgtcagga ttgttttgtg
     6121 ataagtggtt agcaatcata atagttgcta cattagatga cgggacagcc gtcatagtaa
     6181 tcagttcacg aataggataa gcaaccccag catgaatatt gttattacta atttcataaa
     6241 tcttacttat ggcttggtct gtttctgttg cagtcactgt ggtgttgagg tttgtttttc
     6301 ctttagctaa gtcttcaaat agtaaatata aagtaaacat tttagacatg ctagcgggat
     6361 cacgttctaa atcggcgtta tcttcccaca aaatatgacc tttattgtca ataacgatag
     6421 acgctttagg tttattaata tctttaacat catatccggc ttctcgggta atttgcataa
     6481 tatctttatg ttcttcagct tgcacagagt ttacacaagt cagggataat aaaatgcaga
     6541 ggaaaga
//
LOCUS       DATROD010000093         6459 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.095, whole genome shotgun sequence.
ACCESSION   DATROD010000093 DATROD010000000
VERSION     DATROD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.095"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(49..2040)
                     /gene="uvrB"
                     /locus_tag="VBC74_001642"
     CDS             complement(49..2040)
                     /gene="uvrB"
                     /locus_tag="VBC74_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEO6942576.1"
                     /translation="MIDRKDTNRFKLVSKYSPSGDQPQAIETLVDNIEGGEKAQILKG
                     ATGTGKTYTMSQVIAQVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGQEISRDQLLNNLVDIQFERNDIDFQRGKFRVRGDVVEFFPASRDEHAFR
                     IEFFGDEIDRIREIESLTGRVLGEVEHLAIFPATHFMTNDEHMEEAISKIQAEMENQV
                     ELFEKEGKLIEAQRIRQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPFTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYEMEQTDTVVEQIIRPTGLLDPEVEVRPSMGQMDDLLGEINLRTEKG
                     ERTFITTLTKRMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSNGHVIMYADKITDSMQ
                     RAMDETARRRRLQMDYNEKHGIVPQTIKKEIRDLIAITKSNDSDKPEKVVDYSSLSKK
                     ERQAKIKALQKQMQDAAELLDFELAAQIRDVILELKAID"
     gene            complement(2101..3021)
                     /locus_tag="VBC74_001643"
     CDS             complement(2101..3021)
                     /locus_tag="VBC74_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6942577.1"
                     /translation="MNKRILNSQFAVYKNWPSWLLLLFACFMVYLFFFVGLTLGGVGI
                     VLVSSVLYKFSSYTFFEFINLFNNIYGELGTFSFSAFLLLIWVKFVEKRPFSELGFST
                     KFKRTLWSLIKGWSIGFILFSISVITAYILGGLDFHSYDVSKATIFYVVTLLPFWLIQ
                     SGTEELLTRGWLLPLINHRFHLAVAIGVSSTLFGILHLVNAHVTFLSIVSIICSGVLM
                     SLYMIKSGDIWSVAALHGAWNFSQGNLYGIAVSGQKAGASLLHFTVKENAPDWISGGA
                     FGIEGSLISIFVLLAAIIYLLWLIKTEETD"
     gene            3204..5387
                     /locus_tag="VBC74_001644"
     CDS             3204..5387
                     /locus_tag="VBC74_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEO6942578.1"
                     /translation="MKHKLKAFTLALLSIFFVFGGKVSAETVNIVSDTAYAPFEFKDS
                     DQTYKGIDVDIVNEVAKRAGWNVNMTYPGFDAAVNAVQSGQADALMAGTTVTEARKKV
                     FNFSDTYYDTSVILYTKNNNKVTNYKQLKGKVVGVKNGTAAQSFLEENKSKYGYKVKT
                     FDTSDLMNNSLDSGSIYAAMDDQPVVQFAINQGKAYAINMEGEAVGSFAFAVKKGSGH
                     DNLIKEFNTAFAQMKSDGTYNDIMDKWLGKDATKTSGKATGNANEKATPVKPSYKIVS
                     DSSFAPFEYQNGKGKYTGFDMELITKIAKQQGFKLDISNPGFDAALNAVQSGQADGVI
                     AGATITEARQKIFDFSDPYYTSSVILAVKKGSNVKSYQDLKGKTVGAKNGTASYTWLS
                     DHADKYNYHVKAFDEASTMYDSMNSGSIDALMDDEAVLAYAINQGRKFETPIKGEKSG
                     DIGFAVKKGANPELIKMFNNGLASLKKSGEYDKLVKKYLSTASTSSNDKAAKPVDEST
                     ILGLISNNYKQLLSGIGTTLSLTLISFAIAMVIGIIFGMMSVSPSNTLRTISMIFVDI
                     VRGIPLMIVAAFIFWGIPNLIESITGHQSPINDFVAATIALSLNGGAYIAEIVRGGIE
                     AVPSGQMEASRSLGISYGKTMQKVILPQAVRLMLPNFINQFVISLKDTTIVSAIGLVE
                     LFQTGKIIIARNYQSFRMYAILAIMYLVIITLLTRLAKRLEKRLK"
     gene            5387..6127
                     /locus_tag="VBC74_001645"
     CDS             5387..6127
                     /locus_tag="VBC74_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6942579.1"
                     /translation="MAELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLESITSGKVVVDGFELSNPKTDIDKARENIGMVFQHFNLFPHMSVLENI
                     TFAPIELGKESKEAAEKHGMELLEKVGLADKANAKPDSLSGGQKQRVAIARSLAMNPD
                     ILLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGRF
                     LEDGTPEQIFDTPQHPRLQDFLNKVLNV"
     gene            6277..>6459
                     /locus_tag="VBC74_001646"
     CDS             6277..>6459
                     /locus_tag="VBC74_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942580.1"
                     /translation="MKDYINRILHFIKEHMTYHVNFIDDFLDIKWEKVSNIHLRFWTT
                     IIAYLVIFILSISTVIL"
BASE COUNT         2157 a         1219 c         1079 g         2004 t
ORIGIN      
        1 tataaccttc tgctatctcc taaagagaga accagatgag agataagttt aatcgattgc
       61 ttttagttct aagataacat ctcggatttg tgcagctagc tcaaagtcca ataattcagc
      121 tgcatcttgc atttgctttt gaagagcttt aattttagct tgcctttctt ttttagataa
      181 actactgtaa tcgacaactt tttcaggctt atcgctatca ttacttttag taatagcaat
      241 taaatcacgg atttcttttt ttattgtttg tggcacaatt ccatgctttt cattataatc
      301 catttgtaaa cgacgacggc gagcggtctc atccatagct ctttgcatag aatctgttat
      361 tttatcagca tacataataa catgaccatt actgttacgt gctgcacgtc cgattgtttg
      421 gataagacca cgctcattac gaaggaaacc ttccttatca gcatctaaga ttgcaacaag
      481 ggaaacttca ggaacatcta ttccttcacg taagagatta ataccaatta aaacatcaaa
      541 cacaccaagt cgtagatcac gaataatctc tgtacgttct aaggttttaa tatcgctgtg
      601 catatactta actttaactc ccatttcttt gagataatct gttaagtctt ctgccatacg
      661 ctttgtcaat gttgtaataa aggtacgctc acctttttca gtcctcaaat tgatttcacc
      721 aagcaagtca tccatctgcc ccatacttgg tctaacttca acttctggat caagaagtcc
      781 tgttggccgg ataatttgct caacaactgt atctgtttgc tccatttcat agtctcctgg
      841 cgttgcagat acatagacaa tttgatggac atggctttcg aattcttcac gacgaagtgg
      901 acggttatct aaggcagatg gtaaacgaaa accgtaatta accaacattt ctttacgcga
      961 acggtctcca ttatacatcc ctttgatttg ccccattgtc atgtgactct catcaatcat
     1021 aatcaaaaaa tcttctggga agaaatctaa caaagtaaaa ggtggctcac cttcacttct
     1081 accatccatg tgacgtgagt agttctcaac accatttgta tatcccattt cacgaagcat
     1141 ttcaatatca tattctgtcc tttgtcgaat acgttgcgct tcaatcaatt tcccttcttt
     1201 ttcaaaaagt tctacttgat tttccatttc agcttgtatt tttgaaatag cttcttccat
     1261 atgttcatca tttgtcataa agtgtgttgc tgggaaaata gctaaatgtt ccacttctcc
     1321 tagcacacgc cctgttaacg attctatttc tcttatacga tcaatttcat caccaaaaaa
     1381 ttcaattcga aaggcatgct catcacgact cgcaggaaaa aattctacaa catcgccacg
     1441 aacgcgaaat tttccacgtt gaaaatcaat atcgttacgc tcaaattgaa tatcaacgag
     1501 attgttcaat aactgatcac gtgaaatttc ttgacctggc ctaaggctaa caacactgtc
     1561 tgcatactct ttaggagaac ctaaaccgta aatacaagaa acagatgcta caacaataac
     1621 gtcatttctt tctaataacg aagaagtcgc tgagtgacgt aatttgtcaa tttcgtcatt
     1681 gactgagcta tctttttcaa tataagtatc cgaagaaggc acataagctt ctggttgata
     1741 ataatcataa tatgaaacaa agtactctac ggcattgtca ggaaaaaatt ctttaaactc
     1801 tccataaagt tgacctgcta gggttttatt atgtgcaatc accaaggttg gcttgttaac
     1861 ttgggcgata acttggctca ttgtgtaggt tttaccagta ccagtagcac cttttaaaat
     1921 ctgagctttt tctcctcctt caatattgtc aaccaacgtt tcaatagctt gaggttgatc
     1981 tcctgaagga ctatatttgg aaactaattt aaatcggtta gtatcttttc tatctatcat
     2041 ttctttacct cgattacttt attttaacat agaaaaaaga aaaagactga gctgcaacac
     2101 tcaatctgtt tcttcagtct taattagcca tagaaggtat ataattgcag ctaagagaac
     2161 aaaaatagaa atcaaacttc cttctattcc gaatgcacca cctgatatcc agtccggtgc
     2221 gttttcttta acggtaaaat gcaaaagaga tgctcctgct ttctgaccac ttactgcaat
     2281 accatagaga ttaccttggc taaaattcca agctccatgt aaggctgcaa cgctccaaat
     2341 atcaccactt ttaatcatat acaaggacat taaaactccc gaacatataa tacttacaat
     2401 agaaagaaaa gtgacatggg catttaccaa gtgcaaaatg ccaaataaag tggatgagac
     2461 ccctatggca acagctaaat gaaaacgatg attaattaga ggaagaagcc accctcgtgt
     2521 tagcaattct tccgtaccgc tttgaataag ccaaaaagga aggagagtta caacatagaa
     2581 aatcgttgcc ttagaaacat catagctatg aaagtcaagt cctcccaata tataagcagt
     2641 gatgacagaa attgaaaata gaataaaacc tatactccag ccttttataa gtgaccacag
     2701 agttctttta aactttgtag aaaaacccaa ttctgaaaaa ggtctttttt ctacgaattt
     2761 tacccagata agcaaaagga atgctgaaaa actaaaggtt cctaattctc catagatatt
     2821 attaaataaa ttaataaact caaaaaaagt ataggaagaa aacttataga gtacagaaga
     2881 cactagaaca attcccacac cacctagagt tagaccgacg aaaaaaaata ggtataccat
     2941 aaaacaagca aataacaata atagccatga cggccagttt ttatagacag cgaactgact
     3001 atttaaaatc cttttattca taataatcct aaatcctttt tgtgtgatga cactagaaca
     3061 attatatcat aatactaact aaagatatat ataaaattaa cataacatac atgttacgtt
     3121 ttctaacata gaaatgacat tttgttgctc tatcttattt attttgatat aataaaacac
     3181 atatttataa aaaggagatt tacatgaagc acaagttaaa agcttttacg cttgctttac
     3241 tctcaatatt ctttgtgttt ggtggaaagg tcagtgcaga gactgtgaat attgtttctg
     3301 atacagcata cgctccattc gaatttaaag attctgatca aacttataaa ggaatcgatg
     3361 ttgacatcgt taacgaagtc gctaagcgtg ctggctggaa tgttaacatg acgtatccag
     3421 gttttgatgc cgcagttaac gctgttcaat ctggacaggc agatgcgcta atggccggaa
     3481 ctactgttac tgaagcacgt aaaaaagtct ttaatttctc agatacttat tacgatactt
     3541 ccgttattct ttatactaaa aataataata aagtcacaaa ctacaaacaa ctaaaaggaa
     3601 aagtagtcgg tgtaaaaaat ggaacagctg ctcaaagctt cttagaagaa aataaatcta
     3661 aatacggcta taaagttaaa acatttgata caagcgacct aatgaataac agccttgatt
     3721 ctggttctat ttacgccgct atggacgatc aaccagttgt gcaatttgcg ataaatcaag
     3781 gaaaagctta cgccattaac atggaaggcg aagcagttgg tagctttgca tttgctgtca
     3841 aaaaaggtag tggacacgat aatctaatta aagaatttaa cacagctttt gcacaaatga
     3901 aatcagatgg cacttataat gacatcatgg ataaatggct tggaaaagac gctacaaaaa
     3961 caagcggcaa agcaacaggt aatgccaatg aaaaagcaac tcctgtaaag ccaagttata
     4021 aaattgtttc tgattcttca ttcgcaccat tcgaatatca aaacggtaaa gggaaatata
     4081 ctggttttga tatggaatta atcacgaaaa ttgctaaaca gcaaggtttt aaacttgata
     4141 tctcaaatcc aggttttgat gccgctttaa atgctgtcca atctgggcaa gctgacggtg
     4201 ttattgcagg agccacaatc acagaagcac gccaaaaaat ctttgatttt tctgatcctt
     4261 attacacatc tagcgttatc ttagcggtta aaaaaggaag caatgtcaaa tcataccaag
     4321 atttaaaagg aaaaacagtt ggtgctaaaa atggtactgc ctcatatact tggttatcag
     4381 accacgcaga taagtacaac tatcatgtta aagcatttga tgaagcatct acaatgtatg
     4441 atagtatgaa ctcaggttca attgatgctc taatggatga cgaagccgtt cttgcttacg
     4501 ctattaatca aggtcgtaaa tttgaaacac ctatcaaagg tgaaaaatca ggcgatatcg
     4561 gatttgcagt gaaaaaaggg gcaaatccag aattaattaa aatgtttaac aacggtcttg
     4621 cttcactcaa aaaatcgggt gagtacgata aacttgttaa aaaatacctt tccacagcca
     4681 gcacttcttc aaacgataaa gctgctaaac ctgtagatga atcaactatt ttagggttaa
     4741 tttctaataa ctacaaacaa ttgctatctg gtattggaac tactttaagt ttaactctta
     4801 tctcgtttgc gattgctatg gttattggta ttatctttgg tatgatgagc gtatcaccaa
     4861 gtaatactct ccgcacaatt tcaatgattt ttgttgatat tgtccgtggt attccactca
     4921 tgattgtggc cgcttttatt ttctggggta ttcctaattt aatcgaaagc atcacaggtc
     4981 accaaagtcc aattaatgac ttcgttgctg ctactatcgc tctttcttta aatggtggtg
     5041 cgtacattgc tgaaattgta cgtggtggta ttgaagctgt tccttctggt caaatggaag
     5101 caagtcgcag cttaggtatt tcttacggca aaactatgca aaaggttatc ttacctcaag
     5161 cagtacgcct tatgttacca aactttatca accaatttgt catctcatta aaggatacaa
     5221 caattgtatc agcaatcgga cttgtggaac tcttccaaac tggtaaaatc ataattgcac
     5281 gtaactatca atcattccgt atgtatgcta tccttgcaat tatgtatctt gtaatcatta
     5341 cccttctcac ccgtctagca aaacgtttag aaaagaggct taaataatgg cagaattaaa
     5401 aattgatgtc caagatttac acaagtcgta tgggcaaaat gaagttttaa aaggtattga
     5461 tgctaaattt tatgaaggtg atgttgtttg tattattgga ccttctggtt caggaaaatc
     5521 aactttcctt cgcacattga acttacttga atcaatcaca agtggaaaag ttgttgtgga
     5581 cggctttgag ttatcaaatc caaaaacaga catagacaaa gcacgtgaaa atatcggaat
     5641 ggtatttcaa cacttcaatc tttttccaca tatgtcagtt cttgaaaaca tcacttttgc
     5701 tcctatagaa cttggtaaag aatctaaaga agcagctgaa aagcatggga tggaattact
     5761 tgaaaaagtt ggtttagccg ataaagctaa tgccaaacca gatagtttat ctggaggaca
     5821 aaaacaacgt gtagctatcg caagaagctt agcgatgaat ccagatatct tactttttga
     5881 cgaaccaact tctgcacttg atccagaaat ggttggagat gttctcaatg ttatgaaaga
     5941 tttagctgaa caaggtatga ctatgttaat tgtgacacac gaaatgggat ttgctcgtca
     6001 agttgccaat cgtgttatct ttacagatgg tggtcgtttc ctcgaagatg ggacacctga
     6061 gcaaatcttt gatacacctc aacacccacg tctccaagac ttccttaata aagttttaaa
     6121 cgtttaataa aaaaagatga cttcgtgtca tcttttttta tatcctgatc acaaacaaat
     6181 tataatagga taagttttct tctaaaatta gcaaaacatc agctagaatg tttgaataat
     6241 gttgcacaag ggctttcagt atgataatat agcagtatga aagactatat taacagaata
     6301 ttgcacttta ttaaagaaca tatgacttat catgtcaact ttatagatga ctttttagat
     6361 attaaatggg aaaaggtgag taacatccat ttaaggtttt ggacaactat tattgcttat
     6421 ctagttattt ttatcttaag tatctctacc gtaattctt
//
LOCUS       DATROD010000094         6418 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.096, whole genome shotgun sequence.
ACCESSION   DATROD010000094 DATROD010000000
VERSION     DATROD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.096"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(18..947)
                     /locus_tag="VBC74_001647"
     CDS             complement(18..947)
                     /locus_tag="VBC74_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6942581.1"
                     /translation="MVIMINFFRHFWESLKNLKRNFWMTFASVTSVTITLLLVGLFSS
                     VLLNVEKLTTDVSGNFTISAFLNVDSTDAQKQVKDKDGKLKDNPDYHKVYDKIKRISG
                     VEKVTYSSKAEQLKEVQKEYGSDVIDDTYKDALLDVYVVGTSSAKVSKSVSEAIGRIE
                     GVDYTKEPIDSTKLSNLTDNIRIWGFGGVALLIVLAIFLISNTIRMSIMSRRTDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGILGAIVPSLIFYFGYQFVFNKFNPKFETSHVSLYPM
                     DIMVPAIIGGMVIIGIIIGSLGSVLSMRRYLKI"
     gene            complement(931..1623)
                     /gene="ftsE"
                     /locus_tag="VBC74_001648"
     CDS             complement(931..1623)
                     /gene="ftsE"
                     /locus_tag="VBC74_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEO6942582.1"
                     /translation="MALIEMSGVTKKYRRSTTALRNLNLSIQQGEFVYLVGPSGAGKS
                     SLIRLLYREEKLSSGRLKVGEFNLNKLKRRQIPILRRSIGVVFQDYKLLPTKTVYENV
                     AFAMQVIGAKRRHIKKRVPEVLELVGLKHKMRSFPTQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEIAWEIMHLLERINLQGTTVLMATHNSQIVNTLRHRVIEIEAGSVI
                     RDEEKGEYGYHD"
     gene            complement(1642..2740)
                     /gene="prfB"
                     /locus_tag="VBC74_001649"
     CDS             complement(join(1642..2667,2669..2740))
                     /gene="prfB"
                     /locus_tag="VBC74_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEO6942583.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENQMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            complement(2809..3933)
                     /gene="queG"
                     /locus_tag="VBC74_001650"
     CDS             complement(2809..3933)
                     /gene="queG"
                     /locus_tag="VBC74_001650"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEO6942584.1"
                     /translation="MDIKAEIQKLAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVIEDRIYPERLLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPNSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNPLATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGSWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEILEFMSNLTLKDEDSRKELELIRHKWQF"
     gene            complement(4023..5954)
                     /locus_tag="VBC74_001651"
     CDS             complement(4023..5954)
                     /locus_tag="VBC74_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEO6942585.1"
                     /translation="MSNFYKLLKEKFPRKEDIVTEMINLEAICQLPKGTEYFISDLHG
                     EYDAVDYLLRTGAGSIRAKLLDCFDWQKIVAVDLDDFCILLYYPKEKLAFDKMNLSVS
                     AYKTKLWEMIPLQIQVLKYFSSKYTKSKVRKQLPGKFAYIIEELLAEIDRNPEKKSYF
                     DTIIEKLFELDQVEDLIIVLSQTIQVLIIDHLHVVGDIYDRGRYPDRILNRLMAFPNL
                     DIQWGNHDVTWMGAASGSYLCMVNVIRIAARYNNITLIEDRYGINLRRLVDYSRRYYE
                     PLPSFAPILDGEEMTHPDELDLLNMIQQATAILQFKLEAQLIDRRPEFQMHNRQLINQ
                     VNYKDLSISIKGVVHQLKDFNSRCIDSKNPSRLTSEEEELLQQLMIAFQTSESLKKHI
                     DFLFEKGSMYLTYNDNLLFHGCIPMHSNGDFKSFKIAGKTYGGRDLLDLFESQIRLAY
                     ARPEKHDDLATDIVWYLWCGENSSLFGKNAMTTFERYYVSDKVTHQERKNPYFKLRDK
                     DDICTALLQEFDLPKFGHIVNGHTPVKEKNGEQPIKANGKMLVIDGGFAKGYQKNTGL
                     AGYTLIYNSYGIQLISHLPFTSIEEVLSGTNYIIDTKRLVEEAKDRILVKDTTIGQKL
                     TKEIKDLDHLYRHFQEYDD"
     gene            6106..6408
                     /locus_tag="VBC74_001652"
                     /pseudo
     CDS             6106..6408
                     /locus_tag="VBC74_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT         2052 a         1292 c          948 g         2126 t
ORIGIN      
        1 gatttcatct ctttttatta aatcttaagg taacgtctca ttgataggac agatccaagt
       61 gagccaataa taataccaat aatcaccata ccaccaataa ttgctggaac cataatatcc
      121 ataggataca atgaaacatg gcttgtctca aattttggat taaacttgtt aaacacaaat
      181 tgataaccaa aatagaaaat cagcgatgga acgatagccc ctaaaatccc tacccaagca
      241 ccttcaaaaa agaacggtcc tcgaatataa gaatttttag cgccaactaa tctcatgatt
      301 tcaatatctg tacggcgtga cataatcgac atacgaatag tatttgaaat caaaaagata
      361 gcaaggacaa ttaataaggc aacaccacca aatccccata tacggatgtt atctgtgaga
      421 ttagatagtt ttgttgaatc aataggttct ttagtataat caacaccttc aatgcgtcca
      481 attgcttcac tgacactctt agaaactttt gcagaacttg tcccaactac gtaaacatct
      541 aacaaagcat ctttgtaggt atcatcaata acatctgaac catactcttt ttgaacttct
      601 tttaattgtt cagctttact tgaataagtg actttctcta caccagaaat acgtttaatc
      661 ttgtcataaa ctttatgata atcaggatta tcttttagct ttccgtcctt atctttgact
      721 tgtttttgag catccgttga gtcaacattt aaaaaggcac tgatagtaaa attacctgaa
      781 acatcagttg ttaacttttc aacattcagt aaaactgatg aaaacaagcc tacaagaagt
      841 aatgttatgg taactgatgt tacagaagca aaagtcatcc aaaagttacg ttttaagttt
      901 ttaagcgatt cccagaaatg tctaaagaaa ttaatcatga taaccatatt ctcccttctc
      961 ttcatctcgg ataacgctac ctgcttcaat ctcaatgaca cgatgacgaa gtgtgttaac
     1021 aatttgacta ttgtgtgttg ccatcaaaac agtcgttcct tgaaggttaa tacgttccaa
     1081 tagatgcatg atttcccaag cgatttctgg atccaaatta ccagtaggtt catctgcaat
     1141 taataatttt ggattattaa caatcgcacg tgcaattgcc acacgttgtt gttctccacc
     1201 agataactgt gttggaaaag aacgcatctt gtgttttaaa cctactaatt ctaaaacttc
     1261 tggcacgcgt tttttaatat gacggcgctt agctccaata acttgcatcg caaacgcaac
     1321 attctcataa actgtttttg ttggtagtaa tttataatct tggaagacaa ctccaatact
     1381 acgacgtaaa ataggaattt gacgacgttt gagtttattg agattgaatt cccctacctt
     1441 taatctacct gaacttaatt tttcttcacg gtataggagg cgaatcaagc tagatttacc
     1501 agcacctgac ggaccaacta aatacacaaa ctcaccttgc tgaattgata aatttaagtt
     1561 tctcaaggca gttgttgaac ggcgatactt tttagtaaca ccagacattt ctattagtgc
     1621 catgtttctc cttcattatt tttagtcttc aatgcgccac ttaaggtagg catcaataaa
     1681 gccattgatt tctccatcca taaccttatc tacctgagca agctcaaagt tagtacgatg
     1741 atccttcacc atcgtataag gggtgaaaac ataagaacga atctgacttc cccaagtgat
     1801 ttctttttta tcacccttga gagcatctac ttcttgtgct ttcttttctt gttctaactg
     1861 atatagctta gcctgcaaca tcttcatagc gcgatcgcgg ttcccatatt gagtacggtc
     1921 aacggtagac gataccacaa ttccagttgg aatatgcgtc aaacgaacac ctgttgatac
     1981 tttattgacg ttctgaccac cagctcctcc ggaacggaaa gtatccattt taatgtcatc
     2041 atcacgtact tctacttcaa tcgtatcatc taactctggc atcacttcaa ctgaagcaaa
     2101 tgaggtatga cgacgtttag ccgaatcaaa aggtgatata cgaacgaggc gatgaactcc
     2161 catttctgat ttcaacaaac cataagcatt gggaccttca aaagagaggg taaccgattt
     2221 aataccagct tcatcccctg cttgataatc caatacttct actttgaaac cattagcatt
     2281 gccaaaacgg gtatacattc gtaagagtaa atctccccaa tcttgcgctt cagttcctcc
     2341 tgaaccagga tggatttcta agatagcatt attgtggtca taaggctcag ataagagaag
     2401 cgtcatttca taagcaccca ttatcttatc tagctgcatt agattttctt ctaattcttc
     2461 tttcaaagaa tcatcttcgt caagcatctc aagtaagagt tctgtttcgt cagataattc
     2521 ttgcatatta tggaaagtat catacttacc ttttaactca ttcagttctt gagatgtctt
     2581 ttgcgctgca atattgtcat tccaaaagtc tggctcagtc atttgatttt caagaagagc
     2641 aatctcctct tccaaacgat ctaagtcaaa gagacctcct gaagctagtc aacttctctt
     2701 tattttctac tattttttgg cgaatttcag ccacttccat gagatttctc caatctttgt
     2761 taatctgttt tattttatca taaggttctt aaaaatacca taaaaggctt aaaattgcca
     2821 tttatgacgg attaattcca actcttttcg gctatcttca tcttttaagg ttaaattact
     2881 cataaactcc agtatctcat cgtttggttt tttgacaatt tctcccaatg cccatatagc
     2941 tgttgcagta tgaatagggt tattattttt atcaataatc tctattaatt taaccactgc
     3001 ggttttatca tgagcatttg ctagcgcaat aatggcatta cgctgtaaaa tatttttacc
     3061 tcgccaactt ccagcaatca taccaaattt ttctttaaat tgtccatttg ataaactcaa
     3121 aaatggtatt aattctggtt gagccaactc aggatcaatc tctgtcgcca atggattatt
     3181 tatacctttg ttataaggac agcaaatttg gcaaatatcg caaccataga taactgtttt
     3241 aatctttttt cgaaactcta tgtccatcat tcccttatct tgggtttgaa atgatagaca
     3301 acgcttggca ttcattgagc catcaccaat taaacaagaa gttggacagg catctagaca
     3361 acgacgacag tcaccacaat cataatcgac aggcttgtct ggctcgatct ctaaattggt
     3421 aattaattct cccaaaaaca tataagagcc atattcttta gaaataacta gaccattttt
     3481 tcctataaaa ccaatacctg ctctttgggc aactgcagta tcaactaaag ctccagtatc
     3541 gaccattgct ttttgttgca aaggaaaatc tcgacagagc tcttcaatgc cttttgatag
     3601 tctgtctaac ttttcgccga ccacataatg ataatcaagc ccccacgaat taggtgtaat
     3661 ttttcctcgt ttataaggtg ttttttgagg ttgctgagga agtttgtgag gataagcaac
     3721 gccaattgaa ataatcgttt tagccgattc taaaagcctt tcagggtaga tacgatcctc
     3781 tatgacttta tgttcaaatc ctgaattgcg cccctcttcc acagaagcac gcaatgactt
     3841 ttccaaataa tcaaagttat ctgccgtcgt aaaaccaatt ttagagatac caatttcttt
     3901 tgctaatttt tgaatttctg cctttatgtc cataatatta ttttaagtca atttaataaa
     3961 agagacaagg taagaaactg aaaaattaaa tcctacatat caattagtaa attacttaaa
     4021 cactagtcat catattcctg aaaatgacga taaaggtgat ctaagtcttt aatctctttg
     4081 gttaactttt gtccaatagt tgtatctttt acaagaatcc tatccttggc ttcttctact
     4141 aatcgcttcg tatcaatgat atagttggtt ccacttaaaa cttcctcaat ggatgtaaaa
     4201 ggaaggtgtg aaattaattg tatcccatag ctattataaa tcaaagtgta gccagctaaa
     4261 cctgtatttt tctgataacc tttggcaaag ccaccatcaa ttactaacat cttaccattt
     4321 gcttttatcg gttgctcacc atttttttct ttgactggcg tatgaccatt gacaatatgt
     4381 ccaaacttag gcaaatcaaa ctcttgtaaa agggcagtgc aaatatcatc tttatctctc
     4441 agtttaaagt agggattttt tctttcttga tgagttactt tgtcggaaac ataatagcgc
     4501 tcaaaagtag tcatggcatt cttaccaaaa agagaagaat tttcaccaca ccaaagatac
     4561 caaacgatat ctgtcgccaa atcatcatgt ttctctggtc tagcatatgc tagacgtatt
     4621 tgactttcaa ataaatccag taaatcccta ccaccataag tttttcctgc aattttaaag
     4681 cttttaaaat caccattact atgcattggt atgcaaccat ggaataaaag attatcatta
     4741 taggttaaat acatacttcc tttctcaaat aggaaatcaa tatgtttctt taaactttca
     4801 gaagtctgga aggctatcat caattgttgt aaaagctctt cttcctcact tgttaatcga
     4861 ctgggatttt tcgaatcaat gcatctacta ttaaaatctt taagttgatg cacaactccc
     4921 tttatagata tacttaaatc tttataattt acctggttaa taagttgtcg attatgcatt
     4981 tgaaattctg gacgcctgtc aatcaattgg gcttccaatt taaactgtaa aattgctgtt
     5041 gcttgttgta tcatatttaa caaatctaat tcatctggat gagtcatttc ctctccatct
     5101 aatatggggg cgaaagaagg tagaggttca tagtaacggc gtgagtaatc aaccaagcga
     5161 cgcaaattga taccatatcg gtcttcaatt aaagtaatat tattataacg tgcagcaata
     5221 cggataacat ttaccataca aaggtaggaa ccagatgctg ctcccatcca agtgacatca
     5281 tggttccccc attggatatc caaatttgga aaagccatta acctattcaa gatacgatca
     5341 ggatacctac cacgatcgta aatatcccct acaacatgta aatggtcaat gattaaaact
     5401 tgaattgttt gggataatac tataattaaa tcttctactt ggtccaattc aaataatttt
     5461 tcaataatag tatcaaaata gcttttcttc tctgggttac ggtcaatctc agctaaaagt
     5521 tcttcgataa tgtaagcaaa cttacctggc aattgcttac gtactttaga ttttgtatat
     5581 ttactagaaa aatattttaa aacttgtatt tgtaacggaa tcatttccca taacttcgtt
     5641 ttataagcac ttactgacaa gttcatttta tcgaaggcta atttttcctt tgggtaataa
     5701 agcagtatac aaaaatcatc taaatcaaca gcaactattt tctgccaatc aaagcagtct
     5761 aataatttag ctctaataga ccctgcacct gtccgtaaga gataatcaac agcatcgtat
     5821 tccccgtgta aatcactaat aaaatattcc gtccctttgg gtaattgaca aatagcttct
     5881 aaattgatca tttctgttac gatatcttct tttcgaggaa atttttcttt caagagctta
     5941 taaaaattag acatgagacc tccttaaggc accttaaata taaagcttct aaacacaatt
     6001 atgaaagcgc attctacaat gacatatcta ttatagaatg actttaaaac tatttcaaaa
     6061 atttactgtt tatctctttg aactacaata tggtacaata cccttatgac caaacttgct
     6121 atcatgagtg atttacatat tgacctcaat catttttcta cctttgagac gaaaacgtta
     6181 attaatttgc ttaagaaaga aaaaattgat cacctccata tcgctgggga tatatctaat
     6241 cattttacta aagatacact tccctttatc aataacttaa aaaagcatat taaacttagc
     6301 tataatttag gaaaccatga tatgcttgat ttaacagaaa cagaaatcca acgccttgac
     6361 tttcaaacct atcgatttga caaaaaaatg ctactagctt ttcatggcta gtatgatt
//
LOCUS       DATROD010000095         6391 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.097, whole genome shotgun sequence.
ACCESSION   DATROD010000095 DATROD010000000
VERSION     DATROD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.097"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            25..267
                     /locus_tag="VBC74_001653"
     CDS             25..267
                     /locus_tag="VBC74_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HEO6942586.1"
                     /translation="MNTHINITLDMGETSKDLRIPLKISVKQLVMELDSIFSRTERRT
                     KYQLRVINKGILLGENDILAHHPVTTGDRIRIEEFY"
     gene            267..1541
                     /gene="essB"
                     /locus_tag="VBC74_001654"
     CDS             267..1541
                     /gene="essB"
                     /locus_tag="VBC74_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HEO6942587.1"
                     /translation="METDSFVFEEQDFQFEKGPDFWQLSLKRSKVGTQNLQQLQLLEI
                     HHSLLMPMTTAINADTIQFQFQTEAHALSFESFKKETLSEKLRLALNVLDLDKALSLP
                     VNFILHPSNLFLTKNATAKIAYRSLPGIMTPEKFGPEEFLYQFKCFVFALLTQHDYIE
                     LYNGAISVIEVSDFLKSIYHAETIQAVRDIITTDYEQQVEVETHTLAKVSKAKYKLYK
                     YISVWLGALSTILLIPLVYLVFIHNPFKEKMLAADTSFIKVDYNQVINRLEHVKVSKL
                     PYTQKYELAYSYINGMSFSEEQREVILNNVTLKTDELYLDYWINIGRGLDDDAIDAAK
                     RLDDSDLVIYAIVQKMDQVRKDNSLSGKDREQKLSELQTDYDKYWKDRKTALTDEESK
                     SKNSNNHSTNSNKESSESSSTTASTSSKTKSR"
     gene            1548..5977
                     /gene="essC"
                     /locus_tag="VBC74_001655"
                     /pseudo
     CDS             1548..5977
                     /gene="essC"
                     /locus_tag="VBC74_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960154.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
     gene            5982..>6391
                     /locus_tag="VBC74_001656"
     CDS             5982..>6391
                     /locus_tag="VBC74_001656"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942588.1"
                     /translation="MKKQRLLLLFGGLLIMIMMTACKDSKIPENRTKKEYQAEQNFKS
                     YFKYISDKNNYLDNIKVYYFSISISKDVQDKVSETTTCSYRLEKQKNQEFIGNFEHEV
                     SESSQYSTEVKNQIQYPIQYKDNSIRFTEKTPSER"
BASE COUNT         2195 a         1124 c         1153 g         1919 t
ORIGIN      
        1 cttttaaaat aaaggacaaa aaggatgaat acacatatta acattactct agatatgggg
       61 gaaacctcaa aagatttacg tatccctttg aaaattagcg ttaagcaatt agtaatggaa
      121 ttagatagta tttttagccg tactgaaagg cgtactaaat atcaactaag agttataaat
      181 aaaggtattt tattgggaga gaacgatata ttagcgcacc atccagtaac aactggtgat
      241 cgtataagga tagaggagtt ttattaatgg aaacagattc gtttgtattt gaagaacaag
      301 actttcaatt tgaaaaagga ccagattttt ggcagttatc tcttaagcgt tcaaaagtag
      361 gaactcaaaa tctgcaacaa ctgcaattgt tagaaattca ccattctttg ttgatgccta
      421 tgacaactgc aattaatgca gataccattc aatttcaatt ccagacagaa gcgcacgctc
      481 tatcttttga aagctttaaa aaagaaactt tatctgaaaa attacgatta gccttgaatg
      541 tcttggattt agataaggct ttgtctttgc cagtaaattt cattttgcat ccctctaatc
      601 tatttttaac aaaaaatgca actgctaaaa tagcttatcg tagtcttcct gggattatga
      661 cacctgagaa gtttggtcca gaggagtttt tatatcaatt caaatgtttt gtctttgcat
      721 tactgacgca acatgactat atagaattgt ataatggtgc tatttctgtt attgaagtat
      781 cagatttcct aaaaagcatt tatcatgcag aaactattca ggctgttaga gacatcatta
      841 ctactgatta tgagcagcaa gtagaagtag aaactcatac gttagcaaaa gtatcaaagg
      901 caaaatataa gctttataaa tacataagtg tctggctagg agctttatct acaatactct
      961 tgattccgct agtatatctg gtttttattc acaatccatt caaagaaaaa atgttggcag
     1021 cggatacttc atttattaaa gttgattata atcaagtgat taatcgacta gaacatgtaa
     1081 aagtaagtaa gttaccttat acacagaagt acgagttggc ctattcctat attaatggaa
     1141 tgtcattttc tgaagaacag cgtgaagtta ttttaaacaa tgttacgctc aagactgatg
     1201 agctctatct tgattattgg attaatattg gccgtggttt agatgatgat gctattgatg
     1261 ctgccaaacg tttagacgat tctgaccttg ttatttatgc tattgttcag aaaatggatc
     1321 aggttagaaa agacaatagt ttatctggta aagatcgtga acaaaaactt tctgagctac
     1381 aaacagacta tgataaatat tggaaagacc gtaagacagc cttaacagat gaggaatcca
     1441 aatcgaaaaa tagcaataat cattcgacaa attccaacaa agagtcatct gaatcatcaa
     1501 gtactacagc aagtacatct tctaaaacta aaagtaggta ggtggatatg ttagttattt
     1561 gttattatca atcactccgc tacgaattta atcttgaaga ggagaaatcc tttctaatat
     1621 catcaaacgg caagtcgcca ataccagtat cagatttaga aaatgacatc acgctaaaaa
     1681 atatgcaagg tcaattggta tatattattg accaaaaaga aaaagagtta actaatggtg
     1741 tagagatttc aggaattgtc ttctatttag cgaataatca aaaagaaatc tatactccct
     1801 tagattacga agatatatta atcggtgata aagaagggta tcatgtccgt tttaaagaag
     1861 gagcccctaa tttattactg aaaaaaatag aaagcaattg gcagcttaat ttatttgaag
     1921 gtgatattta tctgaataat catcttcaaa aagtagtgca gcagttacct ttaagtttag
     1981 gtgatgagat tagcttccaa ggaacaattg ttaagctttt cccagatgaa attcaaatct
     2041 gggggggggg actgattatg aaacatcatt aactaagaaa gttatgtcag cttatcaatt
     2101 ttacgaaggc tatcctgatt ttcaccgctc acctcgtatt atttatcgtt ccagtgaaga
     2161 taaaattaca gttaatgcgc ctggtaataa acctaataag tctaaagatg aactgttaaa
     2221 attaattgtt cctcctctag tgatgattgg agtcagtatt cttatttcta tttttcgtcc
     2281 tcgtggtatc tatatcattg caacaatgtc aatggctcta gtgacgatga ttttctctat
     2341 cactggttac tttaaaaatc gtaaacagta taagcaggac ttacaagaac gcattgattc
     2401 ctatcatgac tatttatctg ataagtcaat tgaacttcag aagttagcta aagagcaaaa
     2461 gcgtgggcaa cactatcatt acccaacgat tgaaggttta caggaaatgg ctgatactta
     2521 tcaccatcgt atttatgaaa agacaccact tcattttgac ttcttgtatt atcgcctagg
     2581 tttgggagaa gtaccaacga gctataatat tcattattct caaccagaac gttctgggaa
     2641 aaaagatcct ttagagaacg aaggctataa cctctacttt aacaatcgtt atattaaaaa
     2701 tatgccgatt gttgcgaatt tatcacatgg accagttggc tatataggac cacgaggttt
     2761 agtattggag caactccaat tgatggttaa tcaattagct tttttccatt cttaccatga
     2821 tgttcaattt attactattg ttccagagga agagatggat aagtggtctt ggatgcgttg
     2881 gttgccacat gcaaccttac aagatgtcaa tgttcgtggc tttgtttata atcaaagaag
     2941 ccgtgatcaa gtgctcaaca gtcttaacca aattttaaaa ttacgtcgca cacaacgcga
     3001 agataaatct gccaaagaag ggactttatt ttctccgcat tatgtcgtta ttgtaactga
     3061 tgaaaagtta attttagatc acgtcatcat ggaatttttt acggaagatc ctacagaatt
     3121 gggatgttct ctcatctttg ttcaagatgt tatgagttca ctttcagaaa atatcaaaac
     3181 cattattaat attaaagacc gtaatactgg tcagttagtt atagaagaag gagagcttaa
     3241 agaaacagat tttgaattag atcatttctt agaggattat gacaaggaaa atatttcacg
     3301 tcgcctagct ccgttaaatc acctacaaaa tcttaaatct tcaataccag aagcagttac
     3361 ttttatggaa atgtatcaag cagaagaatt tgaagatttg catgttcaag agcgttggat
     3421 aagccatgct ccgtataaaa gcttagctgt tccacttggc ttacgtggtc aggatgatat
     3481 tgtttatcta aacctacatg aaaaagctca tggtcctcat ggattagttg caggaacaac
     3541 gggatcaggg aagtcagaaa ttatccagtc ctatatcctt tctttagctg ttaatttcca
     3601 tccacacgat gtagctttcc ttctcataga ctacaaaggt gggggaatgg caaatctctt
     3661 taaggacctg ccacacttac ttggcaccat caccaactta gatggtgccc aatcgatgcg
     3721 tgctttggtt tctattaatg ctgaattgaa acgtcgtcaa cgtttatttg ctaaagctga
     3781 tgttaatcat attaaccagt atcagaagaa atacaaactt ggagaagttt cagaaccaat
     3841 gcctcatctc ttcttaatct ccgatgagtt tgccgaattg aaatccaacc aacctgagtt
     3901 tatgaaagag cttgtctcaa cagcgcgaat tggtcgttcc ctagggattc acttaatcct
     3961 agcgacccaa aaaccatctg gcgtggtaga tgagcaaatc tggtcaaact cacgcttcaa
     4021 actagctctt aaagtagcag accgcggcga ctccatggaa atgctccata ccccagatgc
     4081 cgccgagatt acacaagcag gacgtgccta cctccaagtt ggtaataatg aagtctatga
     4141 aatcttccaa tcagcatggt caggagcaga ttaccaacct gaaaaagacg accaagggat
     4201 tgaagaccat accatttatt ccatcaacga ccttggccaa tatgaaatcc taaatgatga
     4261 tctttcaggc ttagaccaag ccgaaaatat taaagaagta cctacggaat tagatgccat
     4321 cgttgaaaac attcaaactt taaccaaaga aatgggcatc tcagacttac ctcaaccgtg
     4381 gctaccgcct ctaagcaatc agattgcagt aaccgatctc cgtaaggaag aatcagtaga
     4441 tctctggagt aaagccccat cttacaaagt tgttctgggc tttatggata taccaagcca
     4501 acaagcacaa gaagtagctt accacgattt tgaagatgat gggcatttgt caatttttgc
     4561 aggtccaagt atggggaaat ccacagccct tcaaactgta acattggact tagcacgtca
     4621 caatagtcca gaatttttaa acctttatct ctttgatttt ggtaccaatg gcctcttacc
     4681 attaagacgt ttgccacatg tagcagattt ctttaccatt gatgatgacg aaaaaatagc
     4741 caagtttatc agcagaatca aaacggaaat gtctgaccgt aaaaaagccc tcagtcgcta
     4801 caatgttgca acagcaaaac tttaccgcca ggtatctggg gaaaccatgc ctcaaatcct
     4861 gattgttatt gacagttatg aagggctacg ggaagctcaa acactgacta acttagaagc
     4921 ttgtttccaa aatatttcgc gtgatgggtc gtcacttggc atttccttag ttatctcagc
     4981 tggtcgtatg gcagctttga ggtcgtcctt aatggcaaac cttaaagaac gtatcgctct
     5041 taaattaaca gatgactctg aatcacggac acttgtcggt cgtcaccaac acatcatgga
     5101 agatattcca ggacgcggat taatcaaacg tgatgacatt gaagtgttac aagtggctct
     5161 cccaacagaa ggcactgaaa cctttgacat catcaataac atccaaaacg aatctgacgc
     5221 catgaacagc aaatggactg gaccaagacc aaaagcaata ccaattgttc cagaaacact
     5281 tacctttgag gagtttaagg catatgagga tgttaaaaaa gcaagtgaga ctaatcagat
     5341 ttcacttggt attagcttaa aagaagttga taattgtagc attccacttt cgaaattcaa
     5401 acagttactt tttacttctg acaatccaga taatttgtct aacttgacgc gcttcttagt
     5461 taaatctgtt tcattgatta aagatagtaa ggtttatctt ttagacactg cttctgatta
     5521 tgatgatttg acagaacaac aaattgaata tatcaataat cgaagtgaaa tagcaggaca
     5581 tttagaagct attgtttcag aaatctattt aagagaagaa tctggtaaaa tagaaccatg
     5641 gtttgttatt attcctgata tgaatagttt tgtggcagaa acaaatatgg ataaagaaca
     5701 gattttattc ttacttgact cggctcacaa atatcatatt tatttcttat ttggtggctt
     5761 agcatcatac cttatgacta atatctcaga tgttccaaaa gctattagaa cacaggcaca
     5821 gtatgttctc ttgggaatga gagttatgga tcagtcggta ttaccgaaaa catataattc
     5881 aaaagaacct tatttgaaac cagatatgat ttatattcat gatagaagac aagagacaat
     5941 gcttaaaatc actcaagaaa tagaaatgga gcattgagca tatgaaaaaa caaagactat
     6001 tactgctttt tggaggctta ttaataatga taatgatgac agcatgtaag gattcaaaaa
     6061 tcccagaaaa ccgcacgaaa aaggaatacc aggcagaaca gaattttaag tcatacttta
     6121 aatatatatc agataaaaat aactatttag ataatataaa agtttattac ttttctataa
     6181 gtatttctaa agatgtacaa gataaagtca gtgaaacaac aacttgttca tatagactag
     6241 aaaagcaaaa gaatcaagag ttcattggta attttgaaca tgaagttagt gaatctagtc
     6301 aatattcaac cgaagttaaa aatcaaatac agtatccaat ccagtataaa gataattcaa
     6361 ttcgttttac tgaaaaaaca ccgtcagaac g
//
LOCUS       DATROD010000096         6340 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.098, whole genome shotgun sequence.
ACCESSION   DATROD010000096 DATROD010000000
VERSION     DATROD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.098"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..956
                     /locus_tag="VBC74_001657"
     CDS             <1..956
                     /locus_tag="VBC74_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6942589.1"
                     /translation="PNKKNLGGISELTLTNIFGQDETYHAVTVKVNNPAYPNVTYMTA
                     IVNIDQLVNAKERYEKIIIFVMTTFWIISIGASIYLAKWAQKPIIENYERQKAFVENA
                     SHELRTPLAVLQNRLETLFRKPNATILENSENIASSLDEVRNMRILTTNLLNLARRDD
                     GIKPELAVIKPTLFDSIFENYDLIAQENGKNFTGHNMIQDSFKTDKTLLKQLMTILFD
                     NAIKYTDNDGSIDFTISETDKYLFLEIADNGPGISEEDKVRIFDRFYRVDKARTRQQG
                     GFGLGLSLAQQIVNSLRGNITVIDNKPRGSIFKIKLPKVKG"
     gene            1045..1377
                     /locus_tag="VBC74_001658"
     CDS             1045..1377
                     /locus_tag="VBC74_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEO6942590.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     SGVSRQAVYDNIKRTEKILEAYEMKLHMYSDYIVRSQIFDDILEKYTDDAFLQEKISI
                     LSSIDNRD"
     gene            1395..2960
                     /gene="ffh"
                     /locus_tag="VBC74_001659"
     CDS             1395..2960
                     /gene="ffh"
                     /locus_tag="VBC74_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEO6942591.1"
                     /translation="MAFESLTERLQGVFKNIRGKKKLSEKDVQEVTKEIRLALLEADV
                     ALPVVKTFIKHVRERAVGHEIIDTLDPTQQIVKIVNEELTDLLGAETSEIEKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEDNARPMMIAADIYRPAAIDQLKTLGSQINVPV
                     FDMGTNHSAVEIVTKGLEQARENRNDYVLIDTAGRLQIDATLMQELHDVKAIAQPNEI
                     LLVVDSMIGQEAANVAEEFNRQLSISGVVLTKIDGDTRGGAALSVREITGKPIKFTGT
                     GEKITDIETFHPDRMASRILGMGDLLTLIERASQEYDEKRSMELAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMLPGMANNPAMKNFKVDENEIARKRAIVSSMTPEERENPN
                     LLNPSRRRRIAAGSGNTFVDVNKFIKDFNQAKQMMQGVMSGDMNKMMKKMGIDPNNLP
                     KDMPGMDGMDMSNLEGMMGQNGMPDLSSLGGDMDFSQMFGGGLKGKVGAFAAKQSMKR
                     MANKMKKAKKKRK"
     gene            3027..3713
                     /locus_tag="VBC74_001660"
     CDS             3027..3713
                     /locus_tag="VBC74_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEO6942592.1"
                     /translation="MYLALIGDIINSKQILERETFQQSFQQLMTELSDVYGEELISPF
                     TITAGDEFQALLKQSKKVFQIIDHIQLALKPVNVRFGLGTGNIITSINSNESIGADGP
                     AYWHARSAINHIHDKNDYGTVQVAICLDDEDQNLELTLNSLISAGDFIKSKWTTNHFQ
                     MLEHLILQDNYQEQFQHQKLAQLENIEPSALTKRLKASGLKIYLRTRTQAADLLVKSC
                     TQTKGGSYDF"
     gene            3703..4476
                     /locus_tag="VBC74_001661"
     CDS             3703..4476
                     /locus_tag="VBC74_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEO6942593.1"
                     /translation="MISDFLRDNPILTLLFCAHFLADFQWQSQSLADSKSHSWRGLWR
                     HLLIVFLPLAALMILIPETTLLNLSIWGSHIVIDSIKKLSYPWVEEGHFQKAAFIIDQ
                     LAHYTCIIVFYHALPTYLPPNHWLLPIKHFIVIALVFIIITKPINIVFKIFFNKFQAK
                     ELSSLLTQEKTKIMKEKSEDHEETIEGAGAMIGNLERLIMAILLISGQYAAIGLVFTA
                     KSIARYDKISKSQVFAEYYLIGSLFSIISVLITHWLLLV"
     gene            complement(4509..6104)
                     /locus_tag="VBC74_001662"
     CDS             complement(4509..6104)
                     /locus_tag="VBC74_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6942594.1"
                     /translation="MSSFNRKKLKFLGISLATLTATTVTLVACGNESKNSGDNKVINW
                     YIPTEISTLDISKNTDAYSNLAIGNSGSNLLRIDKEGKPKPDLAKKVSVSSDGLTYTA
                     TLRDNLKWSDGSKLSAEDFVYTWRRIVDPKTASEYAYLATESHLLNADKINSGDIKDL
                     NKLGVTAKGNQVTFKLTSPCPQFKYYLAFSNFMPQKQSYVEKVGKDYGTTSKNQIYSG
                     PYLVKDWNGSNGKFKLVKNKYYWDSKHVKTNSVIVQTIKKPDTAVQMYKQGQIDFAEI
                     SGTSAIYNQTGSVKALTNQKIRQALNLATDRKGVVKAAVDTGSTPAESLVPKKLAKLP
                     NGEDLSKYTAPGYTYNTSKAQKLFKEGLAEVGQSSLKLTITADSDSPAAKNAVDYVKS
                     TWESALPGLTVEEKFVTFKQRLEDAKNENFDVVLFSWGGDYPEGSTFYGLFTTNSAYN
                     YGKFSSKEYDNAYQKAITTDALKPGDAANDYKTAEKALFDQSYYNPVYYLGKKGLQNS
                     KLKGLVRNSTGLNVDFTYAYKTE"
BASE COUNT         2154 a         1083 c         1118 g         1985 t
ORIGIN      
        1 aacctaataa aaaaaatcta ggtggcatta gtgaattaac tttaaccaac atctttggtc
       61 aagatgagac atatcatgct gtcaccgtaa aagttaataa ccctgcttat cctaatgtga
      121 cttacatgac agctattgtt aatattgatc aattagtaaa tgctaaagaa cgctatgaaa
      181 aaattattat atttgtcatg actacttttt ggattatttc aattggagca agtatctact
      241 tagctaaatg ggcacaaaaa ccgattatag aaaactacga aagacagaag gcttttgtcg
      301 aaaatgctag ccacgagctg cgtactcccc tagctgtctt acaaaatcgc ctagaaacgc
      361 tattcagaaa accgaatgcc accattttag aaaatagtga aaatatcgct tcaagtctag
      421 atgaagtacg aaatatgcgt attttaacca ctaatctttt aaacttagca agacgtgatg
      481 atggaatcaa accagaatta gctgttatta agcctacact ttttgatagt atctttgaga
      541 attatgatct aatcgcccaa gagaatggta aaaattttac tggacataat atgatccaag
      601 atagtttcaa aacagataag acactgctaa aacaactcat gactatttta tttgataatg
      661 ctataaaata tactgacaat gatggtagca ttgactttac catatctgaa actgataagt
      721 atctcttttt agaaatcgct gataatggac caggaatttc agaggaggat aaagtcagaa
      781 tttttgatcg tttttaccgt gttgacaaag caagaacacg acaacaagga gggtttggcc
      841 tcggcctttc tttagctcaa caaatcgtta actcactgcg aggtaatatt acagtaattg
      901 ataataaacc tagaggcagt atttttaaga taaaactacc aaaagtaaaa ggttaggcca
      961 tgttagtcct aaccttttaa attttaaagt ggtcttgcat acttgatata gcaagctgat
     1021 aggaaatttg gtataataaa agctatggaa attgaaaaaa caaatcgtat gaatgccctt
     1081 tttgagtttt atgcggcatt attaacagat aaacaaatga attatatcga gttatactac
     1141 gccgatgact acagccttgc tgaaatagct gaagaatccg gtgttagccg acaagctgtc
     1201 tatgataata tcaaacgaac tgaaaaaatt cttgaggcgt acgaaatgaa actacatatg
     1261 tattcagatt atattgtccg tagtcagatt tttgatgaca ttctagaaaa atataccgat
     1321 gatgcctttt tacaagaaaa aatttccatt ctaagtagca ttgataatag agattaaatg
     1381 aggagaaata atttatggct ttcgaaagtt taacagagcg cctgcaagga gtttttaaaa
     1441 atattcgtgg taagaaaaag ttatctgaaa aagatgtgca agaagtcacc aaggagattc
     1501 gtctagctct tttagaagct gacgttgctc ttccagttgt taaaaccttt attaaacatg
     1561 ttcgtgaacg tgctgttgga catgaaatca ttgatacact agatcctacg caacaaattg
     1621 ttaagattgt caatgaagag ctaacagatt tattaggggc tgagacctct gaaattgaaa
     1681 aatcccctaa aattccaacc atcattatga tggttggtct acaaggtgct ggtaaaacaa
     1741 cttttgccgg taagttagca aacaaactaa tcaaagaaga caatgctcgt cctatgatga
     1801 tagcagctga tatttatcga ccagctgcta ttgatcagtt aaaaaccctt ggtagccaga
     1861 ttaatgttcc tgtctttgat atgggaacta accattcagc agttgagatt gtcactaaag
     1921 gtttagaaca ggctcgtgaa aatcgtaatg actatgttct tattgataca gccggacgcc
     1981 tacaaattga tgcaaccctt atgcaggaat tgcatgatgt caaagccata gctcaaccta
     2041 atgaaatact ccttgtcgtt gacagcatga ttggacagga agctgcaaat gtcgctgaag
     2101 aatttaaccg tcagctcagc attagtggtg ttgttctgac aaagattgac ggtgatacac
     2161 gcggtggtgc tgccctttca gtacgcgaaa ttactggaaa accaattaaa tttacaggga
     2221 caggggaaaa aatcactgat attgaaactt tccatcccga tcgtatggca agccgtatat
     2281 tggggatggg agacctgcta acacttattg agcgcgctag tcaagaatac gacgaaaaac
     2341 gttcaatgga attagcggag aaaatgcgtg aaaacacctt tgacttcaac gattttattg
     2401 atcaattaga tcaagttcaa aatatggggc caatggaaga tctccttaaa atgcttccag
     2461 gaatggctaa caatccagca atgaaaaact tcaaagtgga tgaaaacgaa attgctcgca
     2521 aaagagccat tgtgtcatca atgactccag aagagagaga aaatccaaat ctcctcaatc
     2581 caagtcgtag acgtcgtatt gctgctggat caggtaatac tttcgttgat gttaataaat
     2641 tcattaaaga ttttaaccag gcaaaacaaa tgatgcaagg cgtgatgtcc ggtgatatga
     2701 ataaaatgat gaaaaaaatg ggaattgatc ctaataacct ccctaaagat atgcctggaa
     2761 tggacggaat ggatatgtct aatttagaag gtatgatggg gcaaaatggc atgcctgact
     2821 tatctagttt aggtggcgat atggacttca gccaaatgtt tggtggtggc ttaaaaggaa
     2881 aagtaggagc ctttgcagct aaacaatcaa tgaaacgtat ggctaataaa atgaagaaag
     2941 ctaagaagaa acgcaaataa aaatgtctat ttaaatagac atttttattt ttgtctgcta
     3001 taatagacaa aaaggtggtg atatttatgt atttagcatt aatcggtgat atcattaatt
     3061 caaaacagat acttgaacgt gaaactttcc aacagtcttt tcagcaacta atgaccgaac
     3121 tatctgatgt atatggtgaa gagctgattt ctccattcac tattacagct ggtgatgaat
     3181 ttcaagcttt attgaaacaa tcaaaaaagg tatttcaaat tattgaccat attcaactag
     3241 ctctaaaacc tgttaatgta aggttcggcc tcggtacagg aaacattata acatccatca
     3301 attcaaatga aagtatcggt gctgatggtc ctgcctactg gcatgctcgc tcagctatta
     3361 atcatataca tgataaaaat gattatggaa cagttcaagt agctatttgc cttgatgatg
     3421 aagaccaaaa ccttgaatta acactaaata gtctcatttc agctggtgat tttatcaagt
     3481 caaaatggac tacaaaccat tttcaaatgc ttgagcactt aatacttcaa gataattatc
     3541 aagaacaatt tcaacatcaa aagttagccc aactggaaaa tattgaacct agtgcgctga
     3601 ctaaacgcct taaagcaagc ggtctgaaga tttacttaag aacgagaaca caggcagccg
     3661 atctattagt taaaagttgc actcaaacta aagggggaag ctatgatttc tgatttttta
     3721 agggataatc caatcctaac tttattattt tgcgcacatt ttttagctga ctttcagtgg
     3781 caaagtcaat ccttagcaga tagtaaatca catagttgga ggggactctg gagacatctc
     3841 ttgattgttt tcctccccct agcagcttta atgatattga taccagaaac tactttgtta
     3901 aatttaagca tatggggaag tcatattgtt attgatagta taaagaaatt gagttaccct
     3961 tgggtagagg aagggcattt ccaaaaagca gcttttatta tagaccaact agcccactat
     4021 acttgcatta tcgtttttta ccatgcgctt cctacatatt taccgcctaa tcattggctt
     4081 ttgcctatca agcactttat tgtcatagct ctggttttta tcattatcac caaacccatt
     4141 aatattgtgt ttaaaatttt ctttaataaa tttcaagcta aagaattatc atcattatta
     4201 acacaagaaa aaacaaaaat aatgaaagaa aaatctgaag atcatgaaga aacaattgaa
     4261 ggagcaggag ctatgattgg taatttagag cgtctaatta tggctatatt actaatctca
     4321 ggacagtatg ctgctatagg tcttgttttt actgcaaaat ccattgcacg ttacgataaa
     4381 atttctaaaa gtcaggtttt tgccgaatat tatttaattg gatcattgtt ctcaattata
     4441 agcgttttga taactcattg gttacttttg gtatagaaaa gcgctagaaa tatcctaacg
     4501 cttttctttt attctgtctt ataagcataa gtaaaatcaa cattcaaacc agttgaattt
     4561 cttaccaacc cctttaattt agaattttgg agtccttttt tccctaggta ataaactggg
     4621 ttataatatg attgatcaaa taaagctttt tcagcagttt tgtaatcatt agcggcatct
     4681 cctggtttca aggcgtcagt agtaattgcc ttttgatagg cattgtcata ctctttagaa
     4741 gaaaatttac cataattata cgcagaatta gtcgtgaata acccatagaa agttgatcct
     4801 tctggataat ctcctcccca tgagaagagc acgacatcaa aattttcatt ttttgcatct
     4861 tcaaggcgtt gcttgaaagt aacaaatttt tcttcaacag ttagaccagg caaagcactc
     4921 tcccatgtag atttcacata gtcaacagca tttttagctg ctggagagtc cgaatcagct
     4981 gtgattgtta acttgagaga tgactgacca acctcagcaa gaccttcttt aaaaagtttt
     5041 tgagctttac ttgtattata cgtataacct ggggcagtat acttggaaag atcctcacca
     5101 ttaggtaatt tggctaattt tttagggacc agagattcag caggagttga acctgtatca
     5161 actgccgctt taacaacacc tttacgatca gtcgctaaat tcaaagcttg cctaattttc
     5221 tgatttgtca atgctttaac cgaaccagtt tggttataaa tagcagaagt tcctgatatt
     5281 tcagcaaaat caatctgacc ttgcttgtac atttgaactg cagtatctgg ttttttgata
     5341 gtttgaacta tgacactatt tgttttaaca tgtttagaat cccaataata tttatttttg
     5401 actaatttaa atttaccatt tgaaccattc caatccttaa caaggtaagg acctgagtaa
     5461 atttgatttt ttgatgttgt accataatct ttaccaactt tttcaacata cgattgtttt
     5521 tgaggcataa aattagagaa ggctaaataa tatttaaatt gagggcatgg acttgtcaat
     5581 ttaaaggtca cttgattgcc tttagctgtt acaccaagtt tatttaaatc tttaatatct
     5641 ccagaattaa tcttatcagc atttaaaagg tgcgattctg tcgccaagta agcatattca
     5701 gatgcagttt taggatcaac tattctccgc catgtgtaaa caaaatcttc agcgcttagt
     5761 ttgcttccat ctgaccattt taaattatca cgaagtgtag cagtataagt aagaccatca
     5821 gaagaaacgg atactttttt agctaaatcg ggttttggtt tgccctcttt atcaattcta
     5881 agtaaattgc tgcctgaatt acctatagct aagtttgaat aagcatctgt attctttgaa
     5941 atgtccaatg tactgatttc agtaggaata taccaattaa taactttatt atctccagag
     6001 ttctttgact cattaccaca ggcaaccagg gtaactgtgg tggctgttag agtcgccaaa
     6061 gatataccca aaaacttaag tttttttcta ttaaaactag acataatacc agtcctccta
     6121 aacctttttt tataagacat agtagatcta atattgagaa aactagtata ttttaaagta
     6181 gaatttaact ttggttatta acttgcaaat ataattaaaa ataattgagt acactttatt
     6241 accttttcag ataccgataa aatcaatgaa attatgaatc agttttctca aaatactctg
     6301 tctttactat aacaaacatg ttaaacgata ataagtaata
//
LOCUS       DATROD010000097         6118 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.099, whole genome shotgun sequence.
ACCESSION   DATROD010000097 DATROD010000000
VERSION     DATROD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.099"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..96
                     /locus_tag="VBC74_001663"
     CDS             <1..96
                     /locus_tag="VBC74_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEO6942595.1"
                     /translation="DTDNLEKEIPSSDQDTVLAVKSCPTKALTIL"
     gene            complement(83..574)
                     /locus_tag="VBC74_001664"
     CDS             complement(83..574)
                     /locus_tag="VBC74_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEO6942596.1"
                     /translation="MIKLFGKIRYHWQPELSWAIIYWSIAIAPIFIGLSLLYERTEIP
                     SQVFVLFAIFIVLVGIGFHRYFVIEEDGYLRIVSFNFLRRSKFPIEDIAKIEVTKSSV
                     TIKFNNNHERIFYMRKWPKKYFLDALAIEPKFKGEVELLDNLIKMDYFECYRYDKKAL
                     TKS"
     gene            complement(603..1823)
                     /gene="pepT"
                     /locus_tag="VBC74_001665"
     CDS             complement(603..1823)
                     /gene="pepT"
                     /locus_tag="VBC74_001665"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEO6942597.1"
                     /translation="MSYEKLLERFLTYVKINTRSNPNSTQTPTTQSQVDFALTVLKPE
                     MEAIGLKDVHYLPSNGYLVGTLPATSDRLRHKIGFISHMDTADFNAENITPQIVDYKG
                     GDIELGDSGYILSPKDFPNLNNYHGQTLITTDGKTLLGADDKSGIAEIMTAMEYLASH
                     PEIEHCEIRVGFGPDEEIGIGADKFDVKDFDVDFAYTVDGGPLGELQYETFSAAGLEL
                     TFEGRNVHPGTAKNQMINALQLAIDFHSQLPENERPEQTDGYQGFYHLYDLSGTVDQA
                     KSSYIIRDFEEVNFLKRKQLAQAIADNMNEALQSERVKVKLYDQYYNMKKVIEKDMTP
                     INIAKEVMEELDIKPIIEPIRGGTDGSKISFMGIPTPNLFAGGENMHGRFEFVSLQTM
                     EKAVDVILGIVAKD"
     gene            complement(1958..3592)
                     /locus_tag="VBC74_001666"
     CDS             complement(1958..3592)
                     /locus_tag="VBC74_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO6942598.1"
                     /translation="MSQKTTKVSQQEQMVKGTAWLTAGNFISRLLGAIYIIPWYVWMG
                     KHAAEANALFGMGYEIYALFLLISTVGIPVAVAKQVSKYNTLGKEEMSIYLVRKILQF
                     MLILGGVFALIMYIGSPLFASLSKGGQELVPILRSLTLAVLVFPSMSVLRGFFQGFNN
                     LKPYAISQVAEQIIRVIWMLLTAFYIMRLGSGDYIAAVTQSTFAAFVGMFASIAVLLY
                     FLWRYNMLSALISKTPEHIKLDTKEILIETVKEAIPFIITGAAIQIFKLIDQFSFGNT
                     MALFTNYSSEELRVMFAYFSSNPGKVTMILIAVATAIAGVGIPLLTENFVKNDKKAAA
                     RLVVNNLQMLLMFLLPAVAGSVILAKPLYTVFYGLPQGQALGLFVISLIQTIILSIYT
                     VLAPMLQALFENRKAIIYFLYGLVAKVILQVPSIFLFHAYGPLFSTTVALCIPVILMY
                     LKIHEITGFKRQAIRRTSALVLILTLLMSFIISMIIWLMNLVIVPDSRLVSLVYIIVI
                     GAIGLGVYGFMALATHLLDKMIGSRAQDLRRKLHLN"
     gene            3716..5167
                     /locus_tag="VBC74_001667"
     CDS             3716..5167
                     /locus_tag="VBC74_001667"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEO6942599.1"
                     /translation="MITIDKILEILKNDHNFREILFHEHYYYNWTQNVTFNALSYDSR
                     QISSDTLFFAKGATFKKEYLDSAITAGLSFYVSEIDYGADIPVILVNDIKKAMSLISM
                     SFYNNPQNKLKLLAFTGTKGKTTAAYFAYHMLKVNHRPAMLSTMNTTLDGKSFFKSHL
                     TTPESLDLFRMMATAVENQMTHLVMEVSSQAYLTKRVYGLTFDVGVFLNISPDHIGPI
                     EHPTFEDYFFHKRLLMENSNAVVVNSQMDHFNIVKEQVEYIPHDFYGDYSENVITESQ
                     AFSFHVKGKLENTYNINLIGKFNQENALAAGLACLRLGVSIEDIKNGIAQTTVPGRME
                     VLTQTNGAKIFVDYAHNGDSLKKLLAVVEEHQKGDIILVLGAPGNKGQSRRKDFGDVI
                     NQHPNLQVILTADDPNFEDPLVISQEIASHINRPVTIIIDREEAIANASTLTNCKLDA
                     IIIAGKGADAYQIIKGNRDNYSGDLEVAKKYLK"
     gene            5326..>6118
                     /locus_tag="VBC74_001668"
     CDS             5326..>6118
                     /locus_tag="VBC74_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6942600.1"
                     /translation="MSHIKAENIIVSYDQKEIINNLSLSILNQKITTIIGANGCGKST
                     LLKALTRIHKIKDGTITIDGHDIAHLPTKEIAKKIALLPQVLEATEGITVYELISYGR
                     FPHQKYLGNLTNDDRSKIHWAMEMTNVAQFANRDVDDLSGGQRQKVWIAMALAQDTDT
                     IFLDEPTTYLDMNHQLEVLELLKKLNNETQKTIIMVLHDLNLSARYSDYLVAMKTGKI
                     IYEGSPSQIMTKDIIKDIFKIDAHIIQDPISKQPVLLSYQLSTDQN"
BASE COUNT         2105 a         1143 c          963 g         1907 t
ORIGIN      
        1 gatacagata acctagaaaa agaaattcca agttctgatc aagatactgt tttagcagtt
       61 aaatcatgtc ccaccaaagc tcttacgatt ttgtaagagc ttttttatcg tagcgataac
      121 attcaaaata atccatttta atcaaattat cgagaagctc cacttctccc ttaaatttag
      181 gttcaatagc tagtgcatct aaaaaatatt ttttaggcca ttttcgcatg taaaaaatac
      241 gctcatggtt attattaaat ttgatagtga cacttgattt agtgacctca attttagcaa
      301 tatcttcaat tggaaactta gatcgtctta aaaagttaaa agatacaatc ctaagatatc
      361 catcttcttc aataacaaaa tagcgatgaa atccaatgcc tactaaaaca ataaagatgg
      421 caaatagaac aaatacctga ctcggaatct cagttctttc atatagcaga gataaaccaa
      481 taaaaattgg tgcaatggct attgaccaat aaataatagc ccaagaaagt tcaggttgcc
      541 agtgataacg tatcttccca aataatttta tcatttaacc actccaatct gatatttctt
      601 tactaatcct tagcaacgat gcctaaaata acatcaactg ctttttccat tgtttgtaga
      661 ctaacgaatt caaagcgtcc atgcatgttt tcaccacctg caaaaagatt aggagtaggg
      721 attcccataa aggaaatttt agagccatct gtaccaccac gaatcggttc tatgatcggc
      781 ttgatgtcta actcttccat tacttcttta gcaatgttga taggtgtcat gtctttttca
      841 ataactttct tcatgttgta atattgatcg tatagtttaa cctttacacg ttcagattgt
      901 aatgcttcat tcatattatc agcgatagct tgagccaatt gcttacgttt taagaaatta
      961 acttcctcaa aatctcgaat gatatatgaa cttttagctt gatcaactgt tccacttaaa
     1021 tcatataagt gataaaatcc ttgatagcca tctgtttgtt caggacgttc attttctggt
     1081 aattgactat gaaaatctat agcaagctgt aaagcattaa tcatttgatt ttttgcagtt
     1141 ccagggtgaa cgtttcgtcc ttcaaatgta agctccaaac cagctgcact aaaggtttca
     1201 tactgtaatt ctcctagtgg tccaccatcc actgtatagg caaaatcaac atcaaaatct
     1261 ttaacatcaa atttatctgc acctatacca atttcttcat ctggtccaaa gccaactcta
     1321 atttcacagt gctcaatttc tggatgcgaa gccaaatatt ccatagctgt catgatttct
     1381 gctataccag acttatcgtc tgctcccagt aaggttttac catctgttgt aatcaatgtt
     1441 tgcccatggt aattatttaa atttggaaaa tctttcggac ttaaaatgta acctgagtct
     1501 ccaagttcaa tatctccacc tttatagtca acaatttgtg gagtaatatt ttcagcattg
     1561 aaatcagctg tatccatatg ggatataaaa cctattttat ggcgtaagcg gtcgcttgta
     1621 gcaggtaagg ttccaaccaa atacccatta gaaggtaaat aatgaacatc ttttaaacca
     1681 attgcttcca tttctggttt taaaactgtt aaagcaaagt caacttgact ttgagttgtt
     1741 ggcgtttgtg tactattagg attacttctt gtatttattt tgacgtatgt taaaaatcgt
     1801 tctaaaagct tttcgtaaga catctttcac ctcatatttc tccatttaat tataacataa
     1861 aaataagctc actatattgt gactacctta aaatttcaca aataattttg gtcgtcaaaa
     1921 aactaggtta aacaattctg tctaacctat tttttcatta gttaaggtgc aactttcggc
     1981 gtaagtcttg agcacgacta ccaatcatct tatcaagtag atgggtggcc agtgccataa
     2041 agccatatac ccctaaacct attgcaccga taacgataat gtaaactaga cttaccagtc
     2101 tactatcggg tacaataacc aggttcatta accatattat catagaaata ataaagctca
     2161 tcaatagcgt tagaataagg actaaagcac ttgtacgacg aatagcttgg cgtttaaatc
     2221 ctgtaatttc atgtattttc aaatacataa gaattactgg gatacaaaga gctactgtcg
     2281 ttgagaaaag tggtccatag gcatggaaaa ggaaaatgga gggaacttgt aaaataactt
     2341 ttgccaccag accatatagg aagtaaataa tagctttacg attttcaaac aatgcttgta
     2401 gcattggtgc taaaacagta taaatagata aaataattgt ttgaataaga gatatgacaa
     2461 aaagccctaa agcttgtccc tgtggtaacc cataaaagac tgtatacaaa ggttttgcta
     2521 aaataacgga tcctgcaaca gctggcagta aaaacattag taacatttgt aaattattaa
     2581 caaccaaacg agcggcggct tttttatcat tcttaacaaa attctctgtt aacaatggaa
     2641 taccaactcc agcaattgcg gtagcaacag caattagaat catcgtaact ttaccagggt
     2701 tgcttgaaaa gtaagcaaac ataacccgta attcttcact tgaataattc gtaaagagcg
     2761 ccatcgtatt cccaaatgaa aactgatcaa tcaatttgaa aatttgaatt gctgcaccag
     2821 taataataaa agggattgct tccttaacag tctcaattag gatttcttta gtatctaatt
     2881 taatgtgctc tggagtttta ctaatcaagg cagatagcat attataacgc cataagaaat
     2941 aaagcagcac agctatacta gcaaacatac cgacaaaggc ggcgaatgtt gactgtgtca
     3001 cagcagcaat ataatctcct gatcctagac gcataatata gaaagcagtt aataacatcc
     3061 atatgacacg tataatctgc tcagcgactt gactaatggc atacggtttt aaattattaa
     3121 aaccttgaaa aaaaccacgt aaaacactca ttgaaggaaa aactaaaaca gctagagtta
     3181 gactacgcaa tattggaacg agttcttgcc ctcctttact aagtgaagca aatagaggtg
     3241 aaccaatata cataattaat gcaaaaactc cacctaaaat cagcataaac tgcaagattt
     3301 tccgaaccag ataaatgctc atctcttctt tacctaaagt gttatactta gatacttgtt
     3361 ttgcaactgc tactggaatt ccaacagttg atatcaaaag aaataaagca tagatttcat
     3421 atcccatccc aaataaagca tttgcttcgg cagcatgctt tcccatccaa acataccaag
     3481 ggataatata gatggctccc agcaagcgac taatgaaatt ccctgctgtt aaccaagccg
     3541 tccctttgac catttgttct tgctgactaa cttttgttgt tttttgtgac atggtaaact
     3601 ttctattttc aatattattt agcatttaaa agacaaaaat ctcagaaata tcactttatt
     3661 ataaactttt accttagact tgtaaaacga gaacataaag tctaaaataa ggagtatgat
     3721 tacaatagat aaaattttag aaattttaaa aaatgaccat aatttcagag agattttgtt
     3781 tcatgagcat tattattata actggacaca aaatgtaact tttaatgcct tatcatatga
     3841 tagtcgtcaa atttcatctg atactttatt ttttgctaag ggtgccactt ttaaaaaaga
     3901 atatctagat tctgcaatca cagcaggcct ttctttttac gtttctgaga tagattacgg
     3961 cgccgatatt ccagtgatat tagtcaatga tatcaaaaaa gccatgagtt tgatatctat
     4021 gtctttctat aataacccac aaaacaaact taaacttcta gcttttactg gaacaaaagg
     4081 aaaaacaact gcggcttact ttgcttatca tatgctaaaa gtaaaccatc gtcctgctat
     4141 gctctcaact atgaacacaa cattagatgg taaatctttc tttaaatcac atctcacaac
     4201 ccccgagagt cttgatttat ttcggatgat ggctacagcc gttgagaatc aaatgacaca
     4261 cttagtcatg gaagtatcaa gccaagcata cctaaccaaa cgtgtttatg gtttaacctt
     4321 tgacgttggc gttttcttaa atattagtcc cgatcatatt ggtcctatcg agcaccctac
     4381 attcgaagat tattttttcc acaaacggct cttgatggag aatagcaatg ctgtcgttgt
     4441 aaatagtcag atggatcatt ttaatattgt caaagaacag gtagaatata ttcctcatga
     4501 tttctatgga gattattctg aaaatgttat cacagaatca caggcctttt ctttccatgt
     4561 caaaggaaag ttggagaaca cctacaatat caacctgatt ggaaaattca atcaagaaaa
     4621 tgctctcgca gctggacttg cttgtctccg acttggtgtt tcaattgaag atataaaaaa
     4681 cggaattgca caaactaccg tccctggccg catggaagtc ctaacacaaa ccaacggcgc
     4741 aaagattttc gttgactatg cccacaacgg agacagtttg aagaaattat tagctgttgt
     4801 tgaggaacac caaaaagggg atattatatt agtgctcgga gctcctggta ataaaggtca
     4861 aagccgtcgt aaagacttcg gagatgtcat taatcaacat cctaatctac aagtgatact
     4921 cactgcagat gatcctaatt ttgaagatcc cttagttatc tcacaagaaa tcgcaagcca
     4981 tattaatcgt ccggttacta tcatcattga tcgagaagaa gcaattgcta atgccagtac
     5041 tcttactaat tgcaaactag atgcaattat tatcgctggt aaaggggcag atgcttatca
     5101 aattatcaaa ggaaatcgtg acaattactc tggagattta gaagttgcga aaaaatacct
     5161 taaatgataa aaaaagctaa acaataatga ttattgatac tattttgaat cattctaaat
     5221 aattatcatt tcgttcacat ttttcttaat taatgctaaa aaaggctttt gtaagccttt
     5281 tttctatgtt ataatgaact taattttttg ctagaaaggt tctctatgtc tcacataaaa
     5341 gcagaaaaca ttattgtatc ttacgatcag aaagaaatca tcaataatct ttccctcagt
     5401 atacttaacc aaaaaataac aacgattatt ggtgccaatg gatgtggaaa gtctactctt
     5461 ttgaaggctc tcacacggat ccataaaata aaagatggta ctatcactat tgatggtcat
     5521 gatattgcac atttacccac aaaggaaatt gctaagaaga ttgcacttct cccacaggtg
     5581 ttagaagcaa ctgaagggat aacagtttat gaactaatat cttatggacg gttcccacac
     5641 caaaaatacc ttggcaattt gacaaatgat gatcgtagta aaatccattg ggcgatggaa
     5701 atgactaacg tagcgcaatt tgctaataga gatgttgatg acctttcagg aggtcagagg
     5761 caaaaagtat ggattgctat ggctttagca caagatacag atactatctt tttagacgaa
     5821 cctacaactt atcttgacat gaatcatcaa ttagaagtct tagaattgct caaaaaactt
     5881 aataacgaaa cgcaaaaaac aattattatg gtcctacacg acctaaacct gtctgcgcgc
     5941 tattctgatt atctagttgc aatgaaaacc ggtaaaatta tctatgaagg tagtcctagt
     6001 cagatcatga ctaaagatat tatcaaagat atttttaaga ttgatgctca tatcattcag
     6061 gaccctatta gtaaacaacc tgttttgtta tcttatcaat tatcaactga tcaaaatt
//
LOCUS       DATROD010000098         6118 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.100, whole genome shotgun sequence.
ACCESSION   DATROD010000098 DATROD010000000
VERSION     DATROD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.100"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            92..751
                     /locus_tag="VBC74_001669"
     CDS             92..751
                     /locus_tag="VBC74_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HEO6942601.1"
                     /translation="MSLEDYYLLSKKFSQVMTLDSHSSDDGYTIRSLYFDSLEDVDWQ
                     EKEDGVEIRRKIRLRNYGSQSNFAKLEMKQKQGANQKKRSLKLSKTDAQALINGNNSV
                     LLNYPDTFAAECYSRITTQLYQPKTVIEYQRKAFVAKENNIRITFDHHIIGTEASYDI
                     FSEDLNQNYLLDPYLVILEVKYNGFLLSYIKELLDSVDRSELSASKYCLGRAVSKHYR
                     F"
     gene            802..1479
                     /locus_tag="VBC74_001670"
     CDS             802..1479
                     /locus_tag="VBC74_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HEO6942602.1"
                     /translation="MKDLLRNSLEQSGNLSFQDMILHILVAALLSVVIYVSYAYTHSG
                     TAYSKKFNVSLMTLTVLTATVMTVIGNNVALSLGMVGALSVVRFRTAIKDSRDTVYIF
                     WTIVVGICCGVGDYVVAALGSSVIFILLWVMGRVKNENRMLLIVKCDRTLEVDLEGIF
                     FQYFDGKAVQRVKNSTTNTIEMIFEISRKDYDKQLHVDNQLTEKVYQLGNIDYFNIVS
                     QSDEING"
     gene            1472..2917
                     /locus_tag="VBC74_001671"
     CDS             1472..2917
                     /locus_tag="VBC74_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HEO6942603.1"
                     /translation="MGRLMRGKVIYGTTLIGLFLFLFFYFWIPKHHIERIHHHRIKQV
                     DAKSDLTGFKTHLPIISIDTKQQVIPLVTKEGGKYVKARDNINVDIELRDSPSRSHHL
                     SEKPRIRTKGLISYRGNSSRYFDKKSLKVKFVTNKLKEKKHRLAGMPKESEWVLHGPF
                     LDRTLLRNYLSYNIAGEIMSYAPNVRYCELFVNGEYQGVYLAVENIEQGEQRVPIEKS
                     DKKLHKTPYIVAWDREHKAKQKLDNYVHYTHQSGISALDVKYPGKQRLTSKQLEFINK
                     DINHIEKVLYSYDFSQYPKYIDRESFANYFVINEFFRNVDAGKFSTYLYKDLRDRAKL
                     VVWDFNNAFDNQIEGRVDEADFTLTDAPWFNMLIKDKAFIDLVVHRYKELRKGVLATE
                     YLSNYIDETRHFLDPAIDRNYKKWGYVFDLKNTDPRNYLIPTERNVTSYHKSVEQLKD
                     FIKKRGRWMDRNIETLYQYSAPSKNTNTLLE"
     gene            2924..5083
                     /locus_tag="VBC74_001672"
     CDS             2924..5083
                     /locus_tag="VBC74_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942604.1"
                     /translation="MKKYLSIVALLTILFSSIYYLYYFDGSLYLPKGLLKENTRTNFV
                     VKGDTVLHKPTNKPFVVKGVDVESSLAGYHHNDFPITQKTYREWFHLISNMGANTVRV
                     KVPMNVAFYDALYHHNKESKRPLYLLQGIRIDSYRNNASITAFNDNYRGYLKREAKGV
                     VDILHGRKQVWNTDFGSRHYHYDLSPWVLGYVVGDDWNSGTVAYTNHQEKKTQYKGRY
                     FKTSVAANPFEVMLAQVMDELTHYETAKYGWQHLISFSNSPTTDPFHYRKPFEAQAPK
                     YVQLNVENIQANSNVKAGMFAAYKAIDFHPRYKDYLLFDKENISKEDRQKIKELSLSQ
                     GYVKLLNAYHKIPVLVTGYGYSTARGIAQKEIDKRPLPINEKEQGQRLLEDYESFISS
                     GSFGATINAWQDDWNARAWNTSFATNKHSQFLWGDAQVFNQGYGLLGFKNAKHHYQVD
                     GKRGKGEWKHPLMTSATGDDLYASSDESYLYLAIKTKPEKLKEKRLLPIDITPKSGSR
                     KMNGSKVTFSKSSDFVLSIDPNGKSELFVQERYNALKANYLRQLNGKDFYAFPPKKNS
                     SNFEQINMVLRNTKIVEDMEKVKATERFLPTHPTGLLKTGTTDRHQKTFDSQTDISFG
                     KDFIEVRIPWQLLNFSDPSSQKIHDDYFKHYGVKELEIESIALGLGANSKENTLIKMA
                     DYRLKNWERPDTKTFLKDSYYSIKKEWSKERERTYGT"
     gene            5223..>6118
                     /locus_tag="VBC74_001673"
     CDS             5223..>6118
                     /locus_tag="VBC74_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6942605.1"
                     /translation="MALFFTFLKVSQVIFLVLLIAYASFLVISLVIGGVHLYEKNERK
                     KSLTRNKEKIPISIIVPAYNESTTIVSSIDSLLHLDYEAYEIIVVDDGSSDNTSDVLK
                     EEFALMKISNTIDSIIATQTCKDVFQRQVGKVKLTLIVKENGGKGDALNMGINAANYD
                     YFLCLDADSMLQVDSLSQISKSIQVDPTVIAVGGLVQVAQGVKIEQGKVASYRLPWRI
                     IPCAQALEYDSSFLGARIFLDYLRANLIISGAFGLFKKDLVKAVGGYDTQTLGEDMEL
                     VMKLHFFCRNNNIPYRICYETDA"
BASE COUNT         2107 a          882 c         1187 g         1942 t
ORIGIN      
        1 taatttattg ttaatattta tatataaatt aatatttaat taaacagagg tgagtgatga
       61 acgaagttct tagacaggaa aaaaatattt aatatcactt gaagattact atcttttatc
      121 taagaaattt tcccaagtaa tgacgctcga ttcgcatagt tcagacgacg gctatactat
      181 ccgttcgcta tactttgatt ctttagaaga tgttgattgg caagaaaagg aagatggtgt
      241 tgagatacga agaaagattc gtttgcgcaa ttatggtagt caaagcaatt ttgcgaaatt
      301 agaaatgaaa caaaaacaag gtgctaatca aaaaaagcgc tctctaaaat taagcaagac
      361 agatgctcaa gcgctgatta atggtaacaa tagtgtttta ttaaattatc cggatacatt
      421 tgctgcggag tgttattcaa ggataacaac gcaactttat caacctaaaa cggttattga
      481 gtaccaaaga aaagcttttg ttgctaagga aaacaatatc cgaatcacct ttgaccacca
      541 tataataggt acagaggcta gttatgatat tttctcagag gatttaaatc aaaattattt
      601 attagatcct tatcttgtta ttttagaagt taaatataat ggatttttat tgtcttatat
      661 taaagaactc ttagattcgg tagatagaag tgaactttca gcgagtaagt attgtttagg
      721 aagagctgtt agtaaacatt atcgttttta gtgaaaaagt atatacgtta attattatta
      781 aatgtgagag gataacaaaa aatgaaagac ctattacgaa atagtctaga gcaaagtgga
      841 aatttaagtt ttcaagatat gattttacat attcttgtag cagctttatt gagtgtagtt
      901 atttatgttt cctatgctta tacgcatagt ggaactgcct atagtaaaaa gtttaatgtt
      961 tcattaatga cattgacggt cttgactgca acagtaatga ccgttattgg taataatgta
     1021 gccttgtcat tgggtatggt cggtgccttg tcagttgttc gttttaggac agccataaaa
     1081 gattcaagag atacagttta tattttttgg accatagttg ttggtatctg ttgtggtgtc
     1141 ggtgactatg tggtagctgc attaggaagt agcgttatct ttatcttatt atgggttatg
     1201 ggacgtgtta aaaacgagaa tcgtatgtta ttgattgtga agtgcgatag aacactagaa
     1261 gttgatttag aaggaatttt cttccaatat tttgacggaa aagctgttca gcgtgttaaa
     1321 aattcaacaa ctaatactat tgaaatgatt ttcgaaatct ctagaaaaga ttacgataag
     1381 caactccatg tagataatca gttaactgaa aaagtgtacc aattgggaaa tattgattat
     1441 ttcaacattg ttagccaaag cgacgaaatc aatgggtaga cttatgagag ggaaggttat
     1501 ttacggcaca acccttatag gtctttttct attcttattt ttctattttt ggattcctaa
     1561 gcatcacatc gagagaatac atcatcatcg tataaagcag gtagatgcga agagtgattt
     1621 aacaggattt aaaacccatt tgcccattat cagcattgat acaaagcaac aagttattcc
     1681 tcttgttaca aaagaaggcg gaaaatatgt caaagctagg gataatatta atgttgatat
     1741 cgaattacgg gattctccaa gtagatcaca tcatttatca gaaaagccga gaattaggac
     1801 aaaagggtta atatcatata gaggaaattc ctctcgttac tttgataaga agtcattgaa
     1861 agttaagttt gttactaata agttaaagga aaagaagcat cgattagcag gaatgcctaa
     1921 agaatcggag tgggtattgc atggtccctt tctagacaga acattattaa gaaattatct
     1981 gagttataat attgctggtg agattatgtc ctatgcccca aacgttcgct actgtgagtt
     2041 atttgtcaat ggtgagtatc agggagtata tctagcagtt gagaatatcg aacaaggtga
     2101 gcaacgagtt cccattgaaa aaagtgataa aaagttgcat aaaacacctt atatcgttgc
     2161 ttgggatcgt gaacataagg caaagcaaaa gttagataac tatgtacatt atacacacca
     2221 gtctggtatc tcagctcttg atgtgaaata tccaggtaaa caaagattga catcaaaaca
     2281 attagagttt attaataagg acatcaatca tattgaaaaa gtcctttatt cttatgattt
     2341 ttcacaatat cctaaatata ttgatagaga atcatttgca aattattttg tcatcaatga
     2401 attttttaga aatgtggatg cgggtaaatt ttcaacctat ctatataaag atcttcgtga
     2461 tagagcaaaa ctagtagttt gggattttaa taatgctttt gataatcaaa ttgaaggacg
     2521 tgttgatgag gcagatttta ccttgacgga tgcaccgtgg ttcaatatgc tcattaagga
     2581 caaagcattt atagacctgg ttgttcatcg ctataaagaa ttacgaaagg gagttctagc
     2641 gacagaatat ttatctaatt acatagacga gacaaggcat tttttagatc cagctattga
     2701 tcgaaattat aaaaaatggg gctatgtctt tgatttaaag aatactgatc caaggaatta
     2761 tttgattcct actgaacgca atgtgacaag ttatcacaaa tcagtagaac aattaaaaga
     2821 ttttatcaaa aagcgtggca gatggatgga tagaaatatt gagaccttgt atcaatatag
     2881 tgcaccatcg aaaaatacta atactttact ggagtagaac attatgaaaa aatatctaag
     2941 tattgtagcc cttttaacta ttttgtttag ctctatttat tatttgtatt actttgatgg
     3001 tagtttgtat ttaccaaagg gcttattaaa agaaaataca agaactaact ttgttgttaa
     3061 aggtgatact gtacttcaca agcccaccaa taaacctttt gttgttaaag gagtagacgt
     3121 tgagtcttcc ttagcgggtt atcatcacaa cgattttcct attactcaaa aaacgtatcg
     3181 tgaatggttc catttaattt ccaacatggg ggcaaatact gtaagagtca aggtaccgat
     3241 gaatgttgca ttttacgatg ccttatatca ccacaacaaa gaatcaaaga ggccactgta
     3301 tttgttgcaa ggaatacgta tagattctta tcgcaataat gcttctataa cagcttttaa
     3361 tgataattat agggggtatt taaaacgaga agcaaaaggc gttgtggata ttctccatgg
     3421 gcgtaagcaa gtatggaata ctgattttgg tagccgtcat tatcattatg atcttagtcc
     3481 ttgggtactt ggttatgtcg taggggatga ttggaatagt ggtactgtcg cttatactaa
     3541 tcatcaagag aaaaaaacgc aatataaagg acgttatttt aaaacttctg tggcagctaa
     3601 tccatttgag gtcatgctag ctcaagtaat ggatgaattg acacattatg agacagctaa
     3661 atatggttgg caacatttga ttagtttttc aaactcacca acaacagacc cttttcatta
     3721 tcgaaaacca tttgaggcac aggctcctaa atacgtacaa ctaaatgtag aaaatattca
     3781 agctaattca aatgttaaag caggtatgtt tgcagcatat aaagctattg atttccatcc
     3841 tcgatacaag gattatctat tatttgataa agagaatatc agtaaagaag atagacaaaa
     3901 gattaaagaa ctttctttgt cacagggata cgttaaactg ctaaatgctt atcacaaaat
     3961 ccctgttcta gtcacgggtt atggctattc gacagcgaga ggtattgccc aaaaagaaat
     4021 tgataaacgt cctctgccga ttaatgaaaa agaacaaggt cagcgtttac tagaagatta
     4081 tgaatctttt atatcatccg gtagttttgg agcgactatc aatgcatggc aagacgattg
     4141 gaatgcaagg gcgtggaata catctttcgc cacaaataaa catagtcaat tcctatgggg
     4201 ggatgcacaa gtatttaatc aaggttatgg tttattaggc tttaaaaacg caaaacatca
     4261 ttatcaagtt gatggtaaaa gaggcaaagg agagtggaaa catcctctga tgactagtgc
     4321 aacaggagat gacttatatg ctagcagtga tgaaagctat ctctaccttg cgattaaaac
     4381 aaaacctgaa aaactaaaag aaaaacgatt attaccaata gatattacac caaaatctgg
     4441 tagtagaaaa atgaatggta gtaaggtcac attttctaaa tctagtgact ttgtattgtc
     4501 tattgatcca aatggcaagt ctgaattatt tgtccaagag cgctataatg ccttaaaagc
     4561 gaactatctt cgacagctta acggtaaaga tttttatgct ttcccaccaa agaagaacag
     4621 tagtaatttt gagcagataa atatggtatt gagaaataca aagattgttg aagacatgga
     4681 aaaagtaaaa gcaacagaga ggttcttacc aactcatcct actggtcttc tcaaaacagg
     4741 aacaactgat aggcaccaaa aaacatttga ttcacaaaca gatatttcgt ttggaaagga
     4801 ctttatagag gtcagaattc cgtggcagtt gttgaatttt tctgatccat catctcaaaa
     4861 aattcacgat gattacttta aacattatgg tgtgaaggag ttagaaattg agagcattgc
     4921 tttaggatta ggtgctaata gcaaagaaaa cacactgata aagatggcag attatcgttt
     4981 gaaaaattgg gagagacccg ataccaaaac ctttttaaaa gactcctatt atagtattaa
     5041 gaaagaatgg tctaaagaaa gagagagaac atatggtaca taatatttta tatttttaca
     5101 ttttgatttg cttagcaatt attgttttta acattcacta tatcttaaga caacgattag
     5161 ataagcttag ttggcttcgt attcggaaaa aggatataaa tatcaaataa aggggatttt
     5221 ctatggcatt attttttacc ttcctaaagg tatctcaagt cattttctta gtattattga
     5281 ttgcctatgc cagcttttta gtgatttcat tagtcattgg aggtgttcat ctctatgaaa
     5341 aaaatgagcg caaaaaaagt ttaactagaa ataaagaaaa aattccaatt tcaattattg
     5401 taccagccta taatgaatca acaaccattg tgagttcgat tgattcgtta ttgcaccttg
     5461 attatgaagc gtatgaaatt atcgttgtgg atgacggttc cagtgacaat acttcagatg
     5521 ttctcaagga agagtttgca ttaatgaaaa tctcgaatac gattgattca attattgcta
     5581 ctcaaacttg taaagatgtc tttcagagac aggttggtaa ggttaaactg acactcattg
     5641 ttaaagaaaa tggtggcaaa ggggatgcct taaatatggg gatcaatgct gctaattacg
     5701 attattttct atgtctagat gcagattcta tgttgcaggt agattctttg agtcaaatta
     5761 gcaaatccat acaggtagat ccgacggtta tagcagtggg agggcttgtc caagttgccc
     5821 aaggtgttaa gattgagcag gggaaagtag catcttatcg tttgccttgg agaattattc
     5881 cttgtgcaca agcacttgag tatgattcat catttttagg agctcgaatc tttttagact
     5941 atttaagagc taatttaatt atttcggggg ctttcggtct ttttaagaaa gaccttgtta
     6001 aagcggtggg aggatatgac acccagactc taggagaaga tatggagttg gtgatgaaat
     6061 tacatttctt ctgtcgtaat aataatattc cttatcgtat ttgttatgaa acagatgc
//
LOCUS       DATROD010000099         6108 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.101, whole genome shotgun sequence.
ACCESSION   DATROD010000099 DATROD010000000
VERSION     DATROD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.101"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(16..1299)
                     /locus_tag="VBC74_001674"
     CDS             complement(16..1299)
                     /locus_tag="VBC74_001674"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEO6942606.1"
                     /translation="MTKISWTGFSKKSPEERIHYLEEQDFLADSSLEIVTNQDLLSLS
                     LANQMAENVIGRIALPFSLVPDVLVNGKVYQVPYVTEEPSVVAAASFAAKIIKRSGGF
                     LTTVHNRKMIGQVALYDVQDSQHTKESILNQKQQLLEIANAAHPSIVKRGGGACDLTI
                     EIKEDFLIVYLMVDTKEAMGANMVNTMMEALSSPLEDISKGKSLMSILSNYATESLVT
                     ATCRVDLRFLSRQKEEAIKLAQKMTMASQLAQVDPYRASTHNKGIFNGIDAIVLATGN
                     DWRAIEAGAHTYAVKDGQYRGLSRWSYKVDDNCLEGTLTLPMPVATKGGSIGINPSVH
                     LAHDLLGRPNAKELASIILSIGLAQNFAALKALVSTGIQAGHMKLQAKSLALLAGAKE
                     EQISEVVKQLLDSKHMNLETAQKIVNKLTKSKKRI"
     gene            complement(1301..2578)
                     /locus_tag="VBC74_001675"
     CDS             complement(1301..2578)
                     /locus_tag="VBC74_001675"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEO6942607.1"
                     /translation="MRNKTLKVKAFFHIFSFFMVKWNLAKMTFIQKGLPMKIGIDKIG
                     FATSQYVLEMTDLAIARQVDPEKFSKGLLLDSLSITPVTEDIVTLAASAANDILSDED
                     KETIDMVIVATESSIDQSKAASVYVHQLLEIQPFARSFEMKEACYSATAALDYAKLHV
                     EKHPDSKVLVIASDIAKYGIKSTGESTQGAGSIAMLISQNPSILELKEDHLAQTRDIM
                     DFWRPNYSDVPYVNGMFSTKQYLDMLKTTWKVYQKRFNTSLSDYAAFCFHIPFPKLAL
                     KGFNKILDNNLDEQKKAELQENFEHSITYSKKIGNCYTGSLYLGLLSLLENSQNLKAG
                     DQIAFFSYGSGAVAEIFTGQLVDGYQNKLQSDRMDQLNKRQKITVTEYEKLFFEKTIL
                     DENGNANFNTYRTGTFSLDSICEHQRIYKKINN"
     gene            2627..3466
                     /locus_tag="VBC74_001676"
     CDS             2627..3466
                     /locus_tag="VBC74_001676"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEO6942608.1"
                     /translation="MTKADLLFKDNITKIMSEGVFSEQARPRYKNGEMANSKYITGAF
                     AEYDLSKGEFPITTLRPIPIKSAIKEIFWIYQDQTNDLAVLNDKYGVTYWNDWEVGHT
                     GTIGQRYGAVVKKHNIISKLLKQLEDNPWNRRNVISLWDYEAFEETEGLLPCAFQTMF
                     DVRRVNGELYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWRVGKFFYFINNL
                     HIYDNQFEQAQELLKRQPSECNPKLVLNVPDGTDFFDIKPDDFALVDYDPIKPQLRFD
                     LAI"
     gene            3546..4040
                     /locus_tag="VBC74_001677"
     CDS             3546..4040
                     /locus_tag="VBC74_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEO6942609.1"
                     /translation="MTKQIIAIWAEDEDHLIGVNGGLPWRLPKELHHFKETTMGQALL
                     MGRKTFDGMNRRVLPGRETIILTKDEQFQADGVTVLNSVEQVIKWFQEHNKTLFIVGG
                     ASIYKAFLPYCEAIIKTKVHGKFKGDTYFPDVNLSEFKVISRDYFEKDEQNAHAFTVT
                     YFEK"
     gene            4258..5484
                     /gene="clpX"
                     /locus_tag="VBC74_001678"
     CDS             4258..5484
                     /gene="clpX"
                     /locus_tag="VBC74_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEO6942610.1"
                     /translation="MAGNRNNDMNVYCSFCGKSQDEVKKIIAGNGVFICNECVALSQE
                     IIKEELAEEVLADLAEVPKPKELLEILNQYVVGQDRAKRALAVAVYNHYKRVSYTESS
                     DDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENIL
                     LKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTVA
                     SVPPQGGRKHPNQEMIQINTKNILFIVGGAFDGIEDLVKQRLGEKVIGFGQTSRKIDD
                     NASYMQEIISEDIQKFGLIPEFIGRLPVVAALELLTAEDLVRILTEPRNALVKQYQTL
                     LSYDGVELEFDQDALLAIADKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVRI
                     TKAAVEGTDKPVLETA"
     gene            5495..6091
                     /locus_tag="VBC74_001679"
     CDS             5495..6091
                     /locus_tag="VBC74_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEO6942611.1"
                     /translation="MAEEFLNTHNASILLSAANKSHYPQDDLPEVALAGRSNVGKSSF
                     INTLLGRKNLARTSSKPGKTQLLNFYNIDDKLRFVDVPGYGYAKVSKTERAKWGKMIE
                     EYLVTRDNLRVVVSLVDFRHDPSADDIQMYEFLKYYEIPVIIVATKADKIPRGKWNKH
                     ESSIKKKLNFDKKDHFIVFSSVDRTGLDESWDTILSEL"
BASE COUNT         1913 a         1017 c         1202 g         1976 t
ORIGIN      
        1 ttgagatgat ttttctcaaa ttcttttttt acttttagta agtttattga caattttttg
       61 tgctgtctct agattcatgt gtttagagtc tagaagctgt ttaacgactt ctgaaatttg
      121 ttcttcttta gctccagcaa gtagtgccaa cgactttgct tgaagtttca tgtgaccagc
      181 ttgaattcct gtgctaacca atgccttaag agccgcaaaa ttttgagcta gtccaatcga
      241 aagaataata cttgccaact ctttagcatt aggacgacct agtagatcgt gagctaggtg
      301 gacactaggg ttaataccaa tggatccacc ttttgttgca acaggcatcg gtagcgtcag
      361 agtaccttct aggcaattat catcaacctt ataagaccat ctagataatc ctcgatattg
      421 cccatctttg acagcatagg tatgcgctcc agcttcaata gctcgccaat catttccagt
      481 agctaagacg atagcatcaa tgccattaaa aataccttta ttatgtgtgc tagcacgata
      541 agggtctact tgagctaatt gacttgccat cgtcattttt tgagctaact tgatggcctc
      601 ctctttttgg cgacttagaa atcttaaatc aactcggcat gtagcagtca ctaaagattc
      661 ggtagcatag ttagataaaa ttgacattaa gcttttgcct tttgaaatgt cttctaatgg
      721 cgaagataat gcttccatca tggtgttaac catattagca cccatggctt cttttgtatc
      781 aaccataagg taaacaatca aaaagtcttc tttaatctca atagttaggt cgcaagcacc
      841 gcctccacgc ttgactattg atggatgagc agcgttggct atttctaata attgctgttt
      901 ttgatttaat atagattctt tagtgtgttg actatcttgt acatcataaa gggctacttg
      961 tccaatcatt ttacgattat ggacggttgt cagaaaacca ccagatcgtt ttataatttt
     1021 agcagcaaaa cttgcagcag caacaactga cggttcttca gtgacatatg ggacttgata
     1081 gactttaccg ttaaccaaaa catctgggac aagtgaaaat ggtaaggcta ttcgaccgat
     1141 gacattttca gccatttgat tggctagtga taaagagagt aagtcctgat tcgtcactat
     1201 ttctagggaa gaatcagcta aaaaatcttg ttcttcaagg taatgaatac gttcttcagg
     1261 ggattttttt gaaaatcctg tccaggaaat ttttgtcata ttaattatta attttcttgt
     1321 agatacgttg atgttcgcaa atagagtcta gagaaaaagt gccagtccgg taggtgttga
     1381 agttagcatt tccattttcg tctaggattg ttttttcaaa gaagagtttc tcatactcag
     1441 ttactgtaat tttttgacgt ttattgagtt gatccatacg atcgctctga agtttatttt
     1501 ggtagccgtc tactaactga ccggtaaaaa tttcagcaac agctccgcta ccgtatgaaa
     1561 agaaggcaat ttgatcacct gcttttaaat tttgactatt ttctagtaac gatagaagtc
     1621 ctagatacaa tgagccagta tagcaatttc caattttctt actataggta atagagtgct
     1681 caaaattttc ttgtagttca gctttctttt gttcatctaa gttattatct agaattttat
     1741 taaagccctt taaagctaat tttggaaaag gaatatggaa acaaaaagca gcatagtcac
     1801 ttagactagt attaaaacgt ttttgatata ctttccaagt agtttttagc atatctagat
     1861 attgttttgt tgaaaacatg ccattaacat aaggaacatc agaataattt ggtcgccaaa
     1921 agtccataat atcacgggtt tgcgctagat ggtcctcttt tagctctaga atggatggat
     1981 tttgactaat aagcatagcg atactgcctg caccttgagt ggattctcca gtagatttaa
     2041 tgccatattt agcaatatct gaagctatca ctaatacttt agaatctgga tgtttttcta
     2101 catgtaattt agcgtaatca agagcagcag ttgcgctata gcatgcttct ttcatctcga
     2161 atgaccttgc gaaaggttga atctctaaaa gttgatgtac atagacagaa gccgccttac
     2221 tctgatcgat acttgattct gtagcgacaa tcaccatatc aatagtttct ttgtcctcat
     2281 cagataaaat atcattagct gcagaagctg caagtgtaac gatatcttct gtaactggcg
     2341 taatactaag tgaatctaac aataatccct tgctgaattt ttcagggtca acttggcgag
     2401 caatagccaa atcagtcatt tcaaggacat attgactggt tgcaaaacca atcttatcaa
     2461 ttcctatttt cattggtaaa cctttctgaa taaaagtcat ttttgctaaa ttccatttta
     2521 ccataaagaa gctaaaaata tgaaaaaaag ctttaacctt caaagtcttg tttctcacta
     2581 gaatatcttt ttaaaatctg ataaaataag actttggagg tatctcatga caaaagccga
     2641 tttattattt aaagacaata tcacgaaaat tatgtctgaa ggtgtattta gcgagcaagc
     2701 cagacctaga tataaaaatg gggaaatggc taattctaag tatattacag gtgcctttgc
     2761 agagtatgat ttatcaaagg gtgaatttcc tattacaact ctacgtccaa ttccaataaa
     2821 atctgctatt aaagaaattt tctggattta tcaagatcag acaaatgatt tagcagtttt
     2881 gaatgataaa tatggtgtga catactggaa tgattgggaa gttggccata ctggcacgat
     2941 aggtcagcgc tacggtgcag ttgttaaaaa acataatatt atatcaaaac tcttaaaaca
     3001 gcttgaagat aacccttgga atcgtcgtaa tgttatttca ttatgggact atgaagcatt
     3061 tgaagaaaca gaaggcctac taccttgtgc ttttcaaacg atgtttgatg tgagacgcgt
     3121 taatggtgag ctttatttgg atgccacatt aacacaacgt tctaatgaca tgttagttgc
     3181 acatcatatt aatgctatgc agtatgtagc acttcagatg atgattgcaa agcattttgg
     3241 ttggagagtt gggaagtttt tctattttat caataatctt catatttatg ataaccagtt
     3301 tgaacaagct caggaactct taaaacgcca accgtctgag tgtaacccca aactagtatt
     3361 gaatgttcca gatggaacag acttttttga tattaaacca gatgattttg cgttagttga
     3421 ttatgaccca attaaacctc aattacgttt tgatttagca atctaactat ctcatgtttt
     3481 ttaacaagca atttgaggta aaatttgtta gaatatctag agataagtta gtgaggaaaa
     3541 aagtcatgac aaagcaaata attgccattt gggctgaaga tgaagaccat ttgattggag
     3601 ttaatggcgg tttaccatgg aggcttccta aagagttaca tcacttcaaa gaaacgacca
     3661 tggggcaggc tttgcttatg ggacgaaaga cctttgatgg aatgaaccgt cgtgttttac
     3721 ctggtagaga gacaatcatc ttaacaaaag atgaacaatt ccaagcagat ggagtgacag
     3781 tcctaaatag tgttgaacaa gttataaaat ggtttcagga acataataag accttattta
     3841 ttgtaggtgg tgcaagtatt tataaagcat ttctgcctta ttgtgaagca atcataaaaa
     3901 ctaaagttca tggaaaattc aaaggtgata cctattttcc tgatgttaat ctatctgagt
     3961 ttaaagttat ctctagagat tattttgaaa aggatgagca gaatgctcat gcttttactg
     4021 tgacatattt tgaaaaataa ggaaggaata ctattcaatg caacgtagcc tttttggggt
     4081 ctttaccgca tttttagctg tgatttgtgt tttatgtgca attcctgcta taaagaaaaa
     4141 aagatatgga ttaggaacag tttttttgat gaatgctttc actaaccttg tcaacacaat
     4201 ccatgccttt tatggaacat tattttaaat taaatgaata gaaaagttga ggaaaaaatg
     4261 gctgggaatc gtaataacga tatgaatgtc tattgttcat tttgtggcaa aagccaagat
     4321 gaagtaaaaa aaattattgc aggtaatggt gttttcattt gtaatgaatg tgtggcctta
     4381 tcacaagaaa ttattaagga agaattagct gaggaagtac tggctgattt agcagaagta
     4441 ccaaaaccta aggaactatt agaaatatta aatcaatatg ttgtagggca agatcgtgct
     4501 aaacgtgctt tagcagttgc tgtctacaat cattacaagc gtgttagtta taccgagagt
     4561 agtgacgatg atgtagattt gcaaaaatcc aacattttga tgattggtcc aactggctca
     4621 ggaaaaacct tcttagcaca aacactggct aaaagcctta atgtaccgtt tgctattgca
     4681 gatgcgactt cattgaccga agcaggatac gttggagaag atgttgagaa tattcttctt
     4741 aaattgattc aagctgctga ttataatgtc gaacgtgctg agcgtggtat tatctacgtt
     4801 gatgaaatag ataaaattgc taagaaaggc gaaaatgttt ctatcacacg tgatgtgtct
     4861 ggtgaaggtg tacagcaagc ccttcttaaa attattgagg gtacggtagc aagtgttccc
     4921 ccacagggtg ggcgtaaaca tcctaaccaa gaaatgattc aaattaatac caagaacatc
     4981 ctttttattg tcggtggtgc ttttgatggt attgaagacc ttgtgaagca acgtttaggc
     5041 gaaaaagtta ttggttttgg acagacaagc cgtaaaattg atgacaacgc ttcttatatg
     5101 caagagataa tttctgagga tattcaaaag tttggactga ttccagagtt tattggccgt
     5161 ttaccagtag ttgcagcgtt agaacttctt actgcagaag atctggttcg tattctgaca
     5221 gaaccacgca atgctttggt taaacaatac caaaccttat tatcttatga tggtgtagaa
     5281 ttggaatttg accaggatgc tctattggct atcgctgata aggctatcga gcgcaagact
     5341 ggtgcacgtg gtttacgttc tattattgaa gaaacgatgc ttgatatcat gtttgaaatt
     5401 ccaagccaag aagatgtaac aaaagttcgt atcacaaagg ctgctgttga gggtactgac
     5461 aagcctgttt tagagacggc ttagggagat agagatggca gaagaatttt taaatactca
     5521 taatgcgtct attttattga gtgcagctaa caagtcacat tatcctcagg atgatctccc
     5581 agaagttgct ctcgctggtc gttcaaatgt tggtaaatca agctttatca acacgcttct
     5641 tggtcgtaaa aacctagcgc gtacgtcgag taagccaggt aagacacaac tgctcaactt
     5701 ttataacatt gatgacaaac tgcgttttgt cgatgtccct ggatatggct acgctaaggt
     5761 gtctaaaact gagcgtgcta agtggggcaa gatgattgaa gagtaccttg taacacgtga
     5821 taaccttcgt gttgttgtta gtttagttga ttttcgtcat gatccaagtg cggacgatat
     5881 tcaaatgtat gaatttctta agtactatga gattccagta attattgtag cgacaaaggc
     5941 agataaaata cctcgtggca agtggaataa acatgagtcg tctattaaga agaaattaaa
     6001 ctttgataaa aaggaccatt tcatcgtctt ttcatctgtt gaccgcacag gtcttgatga
     6061 atcatgggat actattttat ctgagttata atttgagagt ttatgaca
//
LOCUS       DATROD010000100         6085 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.102, whole genome shotgun sequence.
ACCESSION   DATROD010000100 DATROD010000000
VERSION     DATROD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.102"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..1057)
                     /locus_tag="VBC74_001680"
     CDS             complement(<1..1057)
                     /locus_tag="VBC74_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEO6942612.1"
                     /translation="MHIIIQITIILLASVLATLISKRIGIPAVVGQLLVGIIIGPAML
                     GLVHQNQVLHVLSEIGVILLMFLAGLEANFDLLKKYLKPSLLVAITGVIVPMALFYFL
                     TRLFGFQINTAIFYGLVFAATSISITVEVLQEYNRVKTDTGAIILGAAVADDVLAVLL
                     LSVFIATNGSSSNIGLQIIIQLLFFVFLFICMKYLVPALFKLIEKVHFFEKYTILAIL
                     ICFSLSILADKVGMSSIIGSFFAGLAIGQTSFVDKVEHKISLLSYTFFIPIFFASIAL
                     PLKFDGMMSHLHTILIFTALAVLSKLIPGYFVGRGFNFSKLESLTIGGGMVSRGEMAL
                     IIVQVGLAAKIISSTTYS"
     gene            complement(1327..2889)
                     /gene="guaA"
                     /locus_tag="VBC74_001681"
     CDS             complement(1327..2889)
                     /gene="guaA"
                     /locus_tag="VBC74_001681"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEO6942613.1"
                     /translation="MTDISILNDIQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TADEIRDINPIGIVLSGGPNSVYADGAFGIDEEIFELGIPILGICYGMQLITHKLGGK
                     VLPAGEAGHREYGQSALRLRSESALFAGTPQEQLVLMSHGDAVTEIPEGFHLVGDSVD
                     CPFAAMENTEKQFYGIQFHPEVRHSVYGNDILKNFAVNICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQRAIGDQLTCIFVDHGLLRKNEGDQVMDMLG
                     GKFGLNIIRVDASKRFLDLLSGVEDPERKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGTALGMPDEV
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            3102..3800
                     /locus_tag="VBC74_001682"
     CDS             3102..3800
                     /locus_tag="VBC74_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEO6942614.1"
                     /translation="MLPAYIKIHDAIKKEIDKGTWKIGQRLPSERDLADDYSVSRMTL
                     RQSITLLVEEGILERRVGSGTYVASHRVQEKMRGTTSFTEIVNSQGRKPSSKLISFQR
                     KLANETEIQKLNLSQSDYVVRMERVRYADKVPLVYEVASIPENLIKGFEQSEVTEHFF
                     KTLTENGYEIGKSQQTIYARNASERVASHLEVNAGHAILALTQVSYFTDGKPFEYVHG
                     QYVGDRFEFYLENN"
     gene            3914..5248
                     /gene="trmFO"
                     /locus_tag="VBC74_001683"
     CDS             3914..5248
                     /gene="trmFO"
                     /locus_tag="VBC74_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEO6942615.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEAHRVPAGGAMAVDREGY
                     SEAVTEEIHKHPLIEVIRDEITDIPGDAITVIATGPLTSDSLAAKIHELNGGDGFYFY
                     DAAAPIVDKNTIDMNKVYLKSRYDKGEAAYLNCPMTKEEFMAFHEALTTAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPEDYKGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLNQT
                     FATRKNPNLFFAGQMTGVEGYVESAASGLVAGINAVRRFNGESEVVFPQTTAIGALPH
                     YITHTDSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIATRALKDLEKFLNY"
     gene            5326..6069
                     /locus_tag="VBC74_001684"
     CDS             5326..6069
                     /locus_tag="VBC74_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6942616.1"
                     /translation="MHYKELLESSEKAYLETNRYKHFKNIITSERYFSNYISYKIMPT
                     VSEFLQDMIYLADEQKDYQSDFAFVFFEENAELSSELQRTLSQQGFESEKHIIFTNSL
                     NHLKLFEKEFDDITFTKLNENYLEKYLKFKYKQSIVYGEIYAKQMYNDNRVNLLNNNA
                     KVYLAIKDNQIIGDVTAWDYGEYVEIDDFYVQESYRGQGIGTRLQFDASRGFKKVILI
                     SEEENRKMYEHQGYEEVAYYWTALRSPRK"
BASE COUNT         2126 a         1027 c         1126 g         1806 t
ORIGIN      
        1 cggaataagt agtgcttgaa ataattttag cagctaatcc aacttgaact ataatgagag
       61 ccatctcgcc acgactgacc attccaccac caattgttaa agattctaat tttgaaaaat
      121 taaagcctcg accgacaaag taccctggaa ttaattttga taaaacagcc aaagcagtaa
      181 agattaatat cgtgtgaagg tgagacatca ttccatcgaa tttaagaggt agtgcaattg
      241 aagcgaagaa aattgggata aagaaggtgt aactaagaag tgaaatttta tgttctacct
      301 tatcaacaaa agaagtttgt ccaatagcta atcctgcaaa aaacgaacca atgatagaac
      361 tcattccaac tttatcggct aaaattgata gagaaaaaca gataaggata gcaagaatag
      421 tatatttctc aaagaaatga accttttcga ttaatttgaa aagagcaggt acaaggtatt
      481 tcatacaaat aaataagaaa acaaagaata acaattgaat gataatttgt aatccgatat
      541 tagaagagct tccatttgtt gcaataaaga cactcagaag caaaacagct aagacatcat
      601 cagcaacagc agcacctaaa ataatagcac cagtatctgt ttttacacga ttgtattctt
      661 gtaaaacttc aactgtgata gaaatactag ttgcagcaaa tacgagcccg taaaagatag
      721 cagtattgat ttggaaccca aacaagcgtg tcaagaagta gaagagagcc ataggaacaa
      781 taacaccagt aattgcgacc agtagactgg gcttaagata cttctttaaa agatcaaaat
      841 tagcttcaag acccgctaaa aacatgagta agataacacc aatttctgac aggacatgta
      901 atacctgatt ttgatgaaca agtcctagca ttgcaggtcc gatgatgata cctacaagta
      961 gttgtccaac aacagccggt attccaattc tttttgaaat aagagttgca agaacgcttg
     1021 ccaacaaaat gattgtaatt tgaatgataa tatgcatttt ttcctcctaa tattaataaa
     1081 atgatatcaa tcatattgta ctgaatattt agaataccat tttttaagcc taaaatctag
     1141 gaatatttct tttctgtctt tttcatatta ttatcaagaa aatggaaaaa tatactgaaa
     1201 aatattcaat ttaagaataa tgaacttgta actatcgaac tacataaaaa tgatatttta
     1261 agtgtaagta tcccttattc aagaaataag agtaaaaaaa cttagctact taaagtagct
     1321 aagtttttat tcccactcaa cagttgcagg tggtttactt gtaatatcat aaacaatacg
     1381 gttaacgtga tcaacttcgt taacaatacg tgtcgaaatt ttcttaagta catcccaagg
     1441 aagttgcgca aagtcagctg tcatgccatc aattgaagta attgcacgga tagcaattgt
     1501 atagtcataa gtacgaccat ctcccataac accgacagaa cgtacgccag tattaactgt
     1561 gaaatactgc catacgtcac gatctagacc agctttagcg atttcttcac gaagaatagc
     1621 atctgattcg cggactgttt caagtttttc ttcggtaatt tcacccataa cgcgaatagc
     1681 aagaccaggc ccagggaatg gttgacgcca tacaacttca tcaggcattc caagtgcagt
     1741 accaagagcg cgaacctcat ctttgaataa tgtgttaagt ggctcaatca attcaaattg
     1801 catatcttct ggcaatccac caacattatg gtgtgacttg attgtttggg ctgtttctgt
     1861 cccagattca ataatatctg tgtagagggt accttgagca aggaaatcaa cgcctttaag
     1921 tttacttgcc tcatcatcaa aaacgtacac gaactcattt ccgatgattt ttcgttttct
     1981 ctcgggatct tcaacaccag aaagaagatc taggaagcgt tttgaagcat caacacggat
     2041 aatattaagt ccaaatttac caccaagcat atccataact tggtcacctt catttttacg
     2101 taataaacca tgatctacaa aaatacatgt taattgatca ccgatagcac gttgtaggag
     2161 tactccgaca acagatgaat caacaccgcc agatagtcca agaagtactt tacgatcgcc
     2221 tacagtttca cgtatttttg caatttccat atcaatgaag ttatccattg accaatcacc
     2281 acgtgcacca cagatattaa cagcaaaatt tttaaggata tcattgccat atactgagtg
     2341 acgaacttcg gggtggaatt ggataccata aaattgtttt tcagtatttt ccattgcagc
     2401 aaatggacag tcaacagagt caccaacgag atggaaaccc tcagggattt cagtaacagc
     2461 atcaccatgg ctcatcaaaa caagttgttc ttgaggagtg ccagcaaaga gtgctgattc
     2521 tgagcgtagg cgaagtgctg attgaccata ctcacggtga ccagcttctc ccgcaggaag
     2581 aaccttacca ccgagtttat gggtaatcag ttgcatacca taacaaattc ctaagattgg
     2641 aattcctaat tcaaaaattt cttcatcgat tccaaaagcg ccatcagcgt atacggaatt
     2701 tggaccacct gaaagtacga taccaatagg attaatatcg cgaatttcat cagccgtaat
     2761 tttatgtgat tttaactcag aaaatacacc aaattcacgg atacgacgtg cgataagctg
     2821 gttatattga ctaccgtaat ctagaacgat aattttttgg atatcattca atatagaaat
     2881 atcagtcatt gtttcccttt ctttgtagct aattttaaaa acagctgtac ctataccatt
     2941 ttagcatatt ttatataaat taacatcatt aaaataattg attaaacgct ttcttatcaa
     3001 gaaatattgc ttatgatttt tctgaattat ttcacgattt tagagttaga aatatataga
     3061 agtatggtaa aatgaagtgt agattctagg aggagagaat attgttacca gcatatatta
     3121 aaattcacga cgctattaaa aaggaaatcg ataaagggac ttggaagatt gggcagcggt
     3181 tacctagcga aagagattta gcggatgatt atagtgtaag tcgtatgaca cttcgccaat
     3241 caattacatt gttagttgaa gaaggtattt tggagagacg agttggtagt ggcacctatg
     3301 ttgctagtca tcgtgttcaa gaaaaaatgc ggggaacaac tagttttaca gaaattgtca
     3361 attctcaagg gcgtaagcca tccagtaaac taatctcatt tcaaagaaaa ctagctaatg
     3421 aaaccgaaat tcaaaaatta aatttatcac agtcagatta tgtggttcgt atggaaaggg
     3481 tacgttatgc tgataaggta ccgttggttt atgaggtagc ctctatacca gagaatttaa
     3541 ttaaaggttt tgaacaatca gaggttacag aacatttctt taaaacctta acagagaatg
     3601 gctacgaaat tgggaaaagc caacaaacta tatatgctag aaatgctagt gaacgtgttg
     3661 cttcgcattt agaagttaac gcaggccatg ctattttggc attgacacaa gtttcatact
     3721 ttactgatgg taagcctttt gaatatgttc atgggcaata tgttggagat cgttttgagt
     3781 tttatttaga gaataactag tagtttaatg aataacgttt tttgaagaag tagctatgta
     3841 tgttataatg gaaaaataaa ttatatagaa agatttgagg aagtagttga ctactttcaa
     3901 gaggtattag ttcatgtctc aatcttacat taatgttatc ggcgctggtt tggctggttc
     3961 tgaagcagct tatcaaattg ctaaacgtgg aataccagtt aagctttatg aaatgcgtgg
     4021 ggttaaatca acccctcaac ataaaacaga taattttgca gaattagtat gttccaattc
     4081 attccgcggt gattctctaa caaatgcagt tggtctttta aaagaagaaa tgcgtcgttt
     4141 ggattctatc attatgagaa atggggaggc acatcgagtt ccagcaggtg gagccatggc
     4201 cgttgataga gaaggttatt ctgaagctgt tactgaggaa atccataaac atccattaat
     4261 cgaagtaatt cgtgatgaaa tcacagatat ccctggagat gctattacag ttatcgctac
     4321 aggaccatta acaagtgata gtctggcagc taaaatccat gaacttaatg gtggtgatgg
     4381 tttttatttt tacgatgccg ccgctccaat tgtcgataaa aatacgattg acatgaataa
     4441 agtttacctt aagtctaggt atgacaaagg tgaagcagct tatctaaatt gtccaatgac
     4501 caaagaagaa tttatggctt ttcatgaagc tttaactaca gctgaggaag caccactaaa
     4561 ttcttttgag aaagaaaaat acttcgaagg ttgtatgcct atcgaagtga tggctaagcg
     4621 tggtattaaa acgatgcttt atggtccaat gaaaccagtt ggtttggagt atcctgagga
     4681 ctacaaagga ccgcgagatg gtgaatttaa aacaccatat gctgttgttc aattacgtca
     4741 agataatgct gcaggctctc tttataacat agttggtttc caaactcatc ttaagtgggg
     4801 agaacaaaaa cgtgtatttc aaatgattcc tggtcttgaa aatgctgaat ttgtccggta
     4861 tggtgttatg catcgcaatt cttatatgga ttcaccaaat cttttaaacc aaacctttgc
     4921 tacacgtaag aacccaaatc ttttctttgc tggacaaatg accggtgttg aaggttatgt
     4981 agaatcggca gcaagtggtc ttgtagcggg aatcaatgct gtccgtcgct tcaatgggga
     5041 gagtgaagta gttttcccac aaactactgc tattggtgca ctgccacatt atattacaca
     5101 tacagacagc aaacatttcc aaccaatgaa tgttaacttt ggtatcatca aagaattaga
     5161 aggtccacga attcgtgata agaaagagcg ttatgaagct attgctacac gagctttgaa
     5221 agatttagaa aaatttctca actattgata tattcatttg actttgccaa caagcaaggt
     5281 caaattttct atattgattt ttaaaaatgt atgaatggag taaatatgca ctataaagaa
     5341 ttacttgagt catccgaaaa agcttatttg gaaactaatc ggtataaaca ttttaagaat
     5401 ataatcactt cagagcgtta tttttcaaac tatattagct acaaaataat gcctacagta
     5461 agtgagtttt tacaggatat gatctattta gcagatgaac aaaaagatta tcagtctgat
     5521 tttgcttttg tattttttga ggaaaatgct gaattgagca gtgaattaca gagaacttta
     5581 tcacaacaag gctttgaatc agagaaacat atcatattta caaattcatt aaatcatctt
     5641 aaactttttg aaaaagaatt tgatgacatc acatttacta aacttaatga aaattaccta
     5701 gaaaaatacc taaagttcaa atataaacaa agtatagttt acggagaaat ttacgctaag
     5761 caaatgtata atgataatcg tgttaatctt ttaaataata atgctaaagt ttacctagct
     5821 attaaagata atcaaattat tggagatgta acagcttggg actatggtga atacgtggaa
     5881 atagatgatt tttatgtcca agagtcctac agaggacaag gaattggtac gcgcctacaa
     5941 tttgatgcaa gtagggggtt taagaaagtt atcttaattt cagaagaaga aaacagaaaa
     6001 atgtatgagc accaaggtta tgaggaagtc gcctattatt ggaccgcttt acggagccct
     6061 cgaaagtaaa attataaaag aaatg
//
LOCUS       DATROD010000101         6073 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.103, whole genome shotgun sequence.
ACCESSION   DATROD010000101 DATROD010000000
VERSION     DATROD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.103"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            258..1916
                     /locus_tag="VBC74_001685"
     CDS             258..1916
                     /locus_tag="VBC74_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6942617.1"
                     /translation="MAKQKNNWRRVGVGVLTLASVATLAACGSKSASQDSNGAINWAI
                     PTEINTLDLSKVTDTYSNLAIGNSSSNFLRLDKDGKTRPDLATKVDVSKDGLTYTATL
                     RKGLKWSDGSKLTAKDFVYSWQRLVDPKTASQYAYLAVEGHVLNADKINEGQEKDLNK
                     LGVKAEGDDKVVITLSSPSPQFIYYLAFTNFMPQKQEVVEKYGKDYATTSKNTVYSGP
                     YTVEGWNGSNGTFTLKKNKNYWDAKNVKTKEVRIQTVKKPDTAVQMYKRGELDAANIS
                     NTSAIYQANKNNKDVTDVLEATTAYMEYNTTGSVKGLDNVKIRRALNLATNRKGVVQA
                     AVDTGSKPAIAFAPTGLAKTPDGTDLAKYVAPGYEYNKTEAAKLFKEGLAESGLTKLK
                     LTITADADAPAAKNSVDYIKSTWEAALPGLTVEEKFVTFKQRLEDSRKQNFDIVVSLW
                     GGDYPEGSTFYGLFKSDSQNNDGKFANKDYDAAYNKAISEDAMKPAESAKDYKEAEKI
                     LFEQGAYNPLYFRSGKGLQNPKLKGVIRNTTGLSIDFTHAYKNN"
     gene            2031..2945
                     /locus_tag="VBC74_001686"
     CDS             2031..2945
                     /locus_tag="VBC74_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6942618.1"
                     /translation="MIKYILKRVAILLVTLWVVITLSFFLMQILPGTPYNNPKLTEEM
                     ITLLNKQYGLDKPVWQQYLTYLWNVLHGDFGTSYQSVNQPVSRMISLRLGVSVHLGVQ
                     ALVFGVLGGILVGAISARHKNDKVDGILSVIATLGISMPSFIIGILLLDYFGFKWNLL
                     PLSGWGTFSQTILPSLALGLPTLASVSRFFRSEMIETLNSDYVQLARSKGMTIRQVTR
                     KHAYRNSMIPILTLIGPLAAGLLTGSALIEQIFSIPGIGQQFVTSIPTKDYPVIMGTT
                     IVYAVMLMVAILITDVVISIVDPRVRLQ"
     gene            2955..3986
                     /locus_tag="VBC74_001687"
     CDS             2955..3986
                     /locus_tag="VBC74_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6942619.1"
                     /translation="MADKNRTFKLVGAGSSSTQEKIEKPALSFMQDAWRRLKKNKLAV
                     VSLYLLALLLTFSLASNLFVTQKDANGFDSKKVTTYRNLPPKLSSNLPFWNGSIKYAG
                     NTESTDAYKSQNVPEKVKYALGTDSLGRSVAKRIIVGIRISLLVAIAATFIDLIIGVT
                     YGLVSGFAGGRLDTLMQRVVEVISSIPNLVIVTMLGLVLGNGITAIIISIAFTGWTSM
                     SRQVRNLTLSYREREFVLAARSLGESPIKIAFKHILPNISGIIIVQIMMTIPSAIMYE
                     AVLSAINLGVKPPTASLGSLISDAQENLQYYPYQVILPALALVMISLAFILLGDGLRD
                     AFDPKSSDE"
     gene            3999..5048
                     /locus_tag="VBC74_001688"
     CDS             3999..5048
                     /locus_tag="VBC74_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6942620.1"
                     /translation="MEKETILSVNNLHVDFHTYAGEVKAIRDVNFELKKGETLAIVGE
                     SGSGKSVTTRTLIGLNAKNSEISGNVQFKGRNLVELSEEEWTKVRGNEISMIFQDPMT
                     SLDPTMKIGMQIAEPMMIHQKISKKDALKLALELMKDVGIPNAEEHINDYPHQWSGGM
                     RQRAVIAIALAADPEILIADEPTTALDVTIQAQILNLMKKIQAERDSSIVFITHDLGV
                     VAGMADRVAVMYAGKIVEFGTVDEVFYNPQHPYTWGLLNSMPTTETESGSLESIPGTP
                     PDLLNPPKGDAFAARNEFALDIDHEEEPPYFKVSETHFAATWLLDERSPKVLPPLPIQ
                     KRWEKWNEIEGRKAQ"
     gene            5045..5977
                     /locus_tag="VBC74_001689"
     CDS             5045..5977
                     /locus_tag="VBC74_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6942621.1"
                     /translation="MTENRKKLVEVKNVSLTFNKGKANEVRAIDNVSFDIYEGEVFGL
                     VGESGSGKTTVGRSILKLYDISDGEITFNGEVISHLKGKALHSFRKDAQMIFQDPQAS
                     LNGRMKIRDIVAEGLDIHKLAKSKSDRDSKVQALLDLVGLNKDHLTRYPHEFSGGQRQ
                     RIGIARALAVEPKFIIADEPISALDVSIQAQVVNLMQKLQREQGLTYLFIAHDLSMVK
                     YISDRIGVMHWGKLLEVGTSDDVYNKPIHPYTKSLLSAIPEPDPESERQRVHQPYNPA
                     IEQDGQERQMHEITPGHFVLSTPQEAEEYKKQIL"
BASE COUNT         1987 a         1016 c         1186 g         1884 t
ORIGIN      
        1 taataatttt tttcaaaata acacctttag aaatttttta aactttgtcc ctcctattat
       61 accatatcac tatgtcatac taggaattat tattttcaga taattgaatg aagaaaacgt
      121 ttgttataaa aatgtaaaaa aatgtaaaaa aatgaaagtt aaaaaattat ctgaaaattt
      181 tgcgtttttg tcttttctta agaatttttt gtggtataat gttagtcact agataaatga
      241 attaggagaa caaagctatg gcaaaacaga aaaataactg gcgccgtgtt ggagttggtg
      301 tccttacact tgcttcagtt gcgactcttg ctgcatgtgg aagtaaatca gcttcccagg
      361 attctaatgg agcgattaat tgggctattc caacagaaat caatacacta gatttatcta
      421 aagttacaga cacttactca aatctagcta ttggtaactc tagtagtaat ttccttcgct
      481 tagataaaga tggaaagaca agaccagact tggctactaa agttgatgtt tcaaaagatg
      541 gcttaactta tacagctaca ttacgtaaag gcttgaagtg gtcagatggc agtaaactta
      601 ctgcaaagga ttttgtttat tcatggcaac gtttagttga tcctaaaaca gcttcacaat
      661 atgcttacct tgctgttgaa gggcatgtgc ttaatgccga taaaatcaac gaaggacaag
      721 agaaagactt gaataagcta ggtgttaagg cagaaggcga tgacaaagtt gttattactt
      781 tatctagtcc gtctccgcaa ttcatctact accttgcatt cactaacttc atgccacaaa
      841 aacaagaagt tgttgaaaaa tatggaaaag attacgcaac tacttcaaaa aatacagttt
      901 actcaggacc atatactgtt gaaggttgga atggttcgaa tggtactttc acgctgaaga
      961 aaaacaaaaa ttattgggac gctaaaaatg taaaaacaaa agaagttcgc atccagactg
     1021 ttaaaaaacc agataccgcc gttcaaatgt ataaacgtgg tgagttagat gcagctaata
     1081 tctcaaatac ttctgctatt tatcaagcta ataaaaataa taaagatgtc acagatgttc
     1141 tagaagcgac cactgcctat atggaatata atactactgg ttctgtgaaa gggcttgata
     1201 atgttaagat tcgtcgcgcc ttaaacttag caactaaccg taaaggagtt gttcaagcag
     1261 ccgttgatac aggctcaaaa ccggcaattg cttttgcacc tactggttta gccaaaacac
     1321 cagatggaac tgatttggca aaatatgttg ccccaggtta tgaatataat aaaactgaag
     1381 cagcaaaact ctttaaagaa ggtttggctg aatcaggctt gactaagcta aaattaacaa
     1441 ttacagcaga tgctgatgct cctgctgcca aaaactctgt tgactatatc aagtctactt
     1501 gggaagctgc tcttccagga cttactgttg aagaaaaatt tgtaaccttt aaacaacgct
     1561 tagaagacag tagaaaacaa aactttgaca ttgtagtttc tctttggggt ggggattatc
     1621 cagaaggatc aactttctac ggcctcttta agtcggattc acaaaataac gatggaaaat
     1681 ttgctaacaa ggactatgat gctgcttata acaaggcaat ttctgaagat gcgatgaaac
     1741 cagcagaatc agcgaaggac tataaagaag cagagaaaat tttatttgag caaggtgctt
     1801 ataacccact ctacttccgt agtggtaaag gtttacaaaa tccaaaatta aaaggtgtta
     1861 ttcgtaatac tacaggtttg tcaatagact ttacacatgc ttataaaaat aattaaatta
     1921 ctttacacta aacctttgca agaattggta attgccagtt cttgcttttg gttaagtaaa
     1981 gaaagtgaaa gagtttgcta tttttagcaa ttctaagaaa aggaaatgat atgattaaat
     2041 atattttaaa gcgtgttgct attttgttag taacgctatg ggttgtcata accctatcgt
     2101 tctttctgat gcagatcctt ccaggaacac cctacaataa tccaaaactc actgaagaaa
     2161 tgatcacatt gcttaataaa cagtatggtt tagacaagcc tgtatggcaa caatatttga
     2221 cttacctttg gaatgtactt catggtgatt ttggaacgag ttatcaatca gtgaatcagc
     2281 cagtatcacg tatgattagc ttgagattag gagtttcagt acatctaggt gttcaggctc
     2341 tcgtatttgg tgttttaggc ggtattttgg taggagctat ttcggcacgt cataagaatg
     2401 ataaagtcga tggtatctta agtgttattg caacgctagg gatttctatg ccttcattta
     2461 tcattggtat cttattatta gactattttg gatttaagtg gaacttgttg cctttatcag
     2521 gttgggggac tttctctcaa acaattttac cttctttagc tctaggactc cctacattag
     2581 catctgtttc acgcttcttc cgtagtgaaa tgattgaaac tttaaattct gattatgtgc
     2641 aactagctcg ttcaaaaggt atgacaattc gtcaagtaac tcgaaaacat gcttaccgta
     2701 attctatgat tccaatttta actctaattg gccctttggc ggcgggtctg ttaactggtt
     2761 ctgcactaat tgagcagatt ttctcgattc caggaattgg gcagcagttt gttacttcga
     2821 ttccaacaaa agactatcct gttattatgg ggacaaccat tgtttatgca gtaatgttga
     2881 tggttgctat cttgataaca gacgttgtta ttagtatcgt agacccacgt gttcgcttgc
     2941 agtaaggaga tgttatggca gataaaaaca gaacatttaa acttgtaggt gcaggatctt
     3001 ctagcacaca agaaaaaatt gaaaagcctg ctctttcgtt tatgcaagat gcgtggcgtc
     3061 gcttgaaaaa aaacaaatta gcagtagttt cactctattt attagctctt ttacttactt
     3121 tttcgttagc ctcaaattta tttgtaactc agaaggatgc taatgggttt gattcgaaaa
     3181 aagtaacgac atatcgcaac ttaccaccta aattgagttc aaaccttcct ttttggaatg
     3241 gtagcattaa gtacgctgga aatacagaat ctacagacgc ttataagtct caaaatgtcc
     3301 ctgaaaaagt taagtatgct ttaggtacag attctctagg gagaagtgtt gctaagcgca
     3361 tcatcgtagg tattcgaatt tccttacttg tagctattgc agctacattc attgatttaa
     3421 ttatcggtgt tacatacggt cttgtttcag gttttgctgg aggacgcctt gatacattaa
     3481 tgcaacgtgt cgtagaggta atttcatcaa ttccaaactt agttattgtt acaatgttag
     3541 gtttggtcct tggtaacggt attacggcta ttattatctc tattgctttt accggatgga
     3601 cttctatgtc tcgtcaggta aggaatttaa ccctatcata tcgagaaaga gagtttgtgc
     3661 ttgctgcacg ttcacttggg gaaagtccaa ttaaaatagc cttcaaacat attttaccta
     3721 acatttctgg tattatcatt gtacaaatta tgatgacaat cccaagtgct attatgtatg
     3781 aagcggtact ttcagcaatc aatttaggtg ttaaaccacc gacagcttca ttaggatcat
     3841 tgatttcaga tgcacaagaa aatttacaat attatccata tcaagttatc ttgccagctt
     3901 tagcattggt aatgatttca ttggcattta ttttgcttgg tgatgggttg cgagatgctt
     3961 ttgatccaaa atctagcgat gaataaggag gtataatcat ggaaaaagaa actattttaa
     4021 gtgttaataa tcttcatgtt gacttccaca catatgctgg agaagtaaaa gcaattcgtg
     4081 atgtcaactt tgaattaaaa aaaggtgaga ctcttgcaat cgttggtgaa tctggttcag
     4141 gaaaatctgt aactaccaga actttaattg gtttaaatgc taaaaattca gagatatcag
     4201 ggaatgttca atttaagggg cgtaaccttg ttgaactatc agaagaagag tggactaagg
     4261 tacgcgggaa tgagatttct atgattttcc aagaccctat gactagtttg gatccaacga
     4321 tgaaaattgg tatgcaaata gcggagccaa tgatgattca tcaaaaaatt tcaaaaaagg
     4381 atgctttaaa attagctctt gagctaatga aagatgtagg tatcccaaat gctgaagagc
     4441 atattaacga ttacccacat cagtggtctg gaggaatgcg tcaacgtgcg gttatagcga
     4501 ttgctttagc tgctgatcct gaaattctta ttgctgatga accaacgact gctttagatg
     4561 taacaattca agcacaaatt ttaaacttaa tgaaaaaaat tcaagcagaa cgagactcct
     4621 ccattgtttt cataactcat gacttagggg tcgttgcagg gatggcagac cgtgtagcag
     4681 ttatgtatgc agggaaaatc gttgaatttg gaactgttga tgaagtcttt tataatccac
     4741 aacatccata tacttgggga ttattgaatt caatgccgac aaccgaaaca gaatcaggta
     4801 gtttagagtc aattccggga acaccaccag atttgttgaa cccacctaaa ggagatgcat
     4861 ttgcagctcg taatgaattt gcattagata ttgatcatga agaagaacca ccgtacttca
     4921 aagtttcaga aacacatttc gctgctacat ggcttttaga tgaaagatca cctaaggtgc
     4981 ttcctccatt accgatccaa aaacgttggg aaaaatggaa cgagattgaa gggaggaaag
     5041 cccaatgact gaaaatcgaa aaaaattagt tgaagtcaaa aatgtttctt tgaccttcaa
     5101 taaaggaaaa gctaatgaag tgagagcaat tgataatgtt agttttgaca tttatgaagg
     5161 tgaagtgttt ggattagttg gggaatctgg atcaggaaag acaaccgttg gacgttcaat
     5221 tttaaaactt tatgatattt ctgatggcga aattaccttt aatggagaag tgatctcaca
     5281 tctgaaaggt aaagcattac atagttttcg taaagacgct cagatgattt ttcaggatcc
     5341 tcaggctagc ttaaacggcc gtatgaagat tcgtgatatc gtagcagaag gtttagacat
     5401 acataaatta gctaaatcaa aatcagatcg tgatagtaaa gttcaggccc tattagatct
     5461 tgtagggtta aataaagatc atttaacacg ttatccgcat gaattttcag gtggacaacg
     5521 acaacgtatc gggattgcac gtgctttagc agtagagcct aaatttatca ttgctgatga
     5581 accaatatct gccttagatg tttcaatcca agcacaagtt gttaatttaa tgcaaaaatt
     5641 gcaaagagaa caaggattga cttatttgtt tatcgcacat gatttgtcta tggtaaaata
     5701 catttcagat cgtatcggtg ttatgcattg ggggaaactg ttagaagttg gaacatctga
     5761 tgatgtttat aacaaaccaa tccaccctta tacgaagagt ctattatcag ctattccaga
     5821 accagatccg gagagtgaac gtcaacgtgt tcaccagccg tataatccag ctattgagca
     5881 agacggacaa gaacgtcaaa tgcacgagat tacacctggt cattttgttt tatctacacc
     5941 ccaagaagct gaagaatata aaaaacaaat attataaaag atggctagcc catcttttat
     6001 ttttttacta ataaaataaa tttaggtatg gaattttaaa ctctatgtta ttggtgttag
     6061 ccaacaggaa act
//
LOCUS       DATROD010000102         6046 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.104, whole genome shotgun sequence.
ACCESSION   DATROD010000102 DATROD010000000
VERSION     DATROD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.104"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..306)
                     /locus_tag="VBC74_001690"
     CDS             complement(<1..306)
                     /locus_tag="VBC74_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CylD"
                     /protein_id="HEO6942622.1"
                     /translation="MKTVIIYGGQGNVQSKHLCQLVKTPKGEILFSKLQATDLESYQL
                     MMDVINHKVSLDNVHASMLANFLYNDWLTNDEKIVSGIGFSAHSAGIFNVLYASGSTS
                     "
     gene            complement(306..611)
                     /locus_tag="VBC74_001691"
     CDS             complement(306..611)
                     /locus_tag="VBC74_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cylX protein"
                     /protein_id="HEO6942623.1"
                     /translation="MGRSNILSRCEQCSDNQCGLYKTRLKDKLLLVVQLVGNIRYSEF
                     LTEGGVYHKGETLLEVITLGISYEYKSYGDIKVIKKYCADGSVVDYNMPILLMERIE"
     gene            complement(1236..2021)
                     /locus_tag="VBC74_001692"
     CDS             complement(1236..2021)
                     /locus_tag="VBC74_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6942624.1"
                     /translation="MTKYQRMHFIFIKQYMKQIMEYKIDFFVGVLGVFLTQGLNLLFL
                     NVLFQRIPSLEGWTFQQIAFIYGFSLLPKGIDHLFFDNLWALGQRLIRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGFILLSTTVSSISWIVPKVLLFIFIIPFATLIYTS
                     LKIATSSIAFWTKQSGAVIYIFYMFNDFAKYPVAIYNNLLRWIISFVIPFAFTAYYPA
                     AYFLQDRNVYFNIGGVILISLISFMVSLILWHKGVEVYESAGS"
     gene            complement(2023..2841)
                     /locus_tag="VBC74_001693"
     CDS             complement(2023..2841)
                     /locus_tag="VBC74_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEO6942625.1"
                     /translation="MNRIWRRYKPFISTEIQGLITYRVDFILYRIGDVIGAFVAFYLW
                     KAVFDSSSQSLIQGFQLSDMILYIIMSFVTNLLTRTDSSFMIGDEVKDGSIIMRLLRP
                     VHFAASYLFMEIGSRWLIFLSIGVPFLLVITGVRLFLGTDLIQAIVLVVFYIISIILA
                     FLINFFFNICFGFSAFVFKNLWGSNLLKNSLVAFMSGSLIPLTFFPKIVADILGFLPF
                     SSLIYTPVMIIIGKYDGSQIVQALLLQIFWLIVMVALSQLIWKKVQSHITIQGG"
     gene            complement(2844..3836)
                     /locus_tag="VBC74_001694"
     CDS             complement(2844..3836)
                     /locus_tag="VBC74_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6942626.1"
                     /translation="MSMIEVSHLQKNFIKTVKAPGLKGAFQSFLRPEKHTFEAVKDLT
                     FDVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRIDGKLPQENRQNYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDKEFRKRMAFLNEVLELNDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCHRIFMIDRGQEIFDGTVSQLKETFGKMKTLSFDLRPGQEHISSSLIGKSEIN
                     IKRNDLVLDIQYDSSRYQTADIIQQTLADFSVRDLKMTDADIEDIIRRFYRNEL"
     gene            complement(4036..5187)
                     /locus_tag="VBC74_001695"
     CDS             complement(4036..5187)
                     /locus_tag="VBC74_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6942627.1"
                     /translation="MVRKFLKSLLSLFLIAVIATGISVACFFFIPENKGNITPILLHR
                     FMRKNNVNGMMIVSDNTGKPITISHGINRGEVETDIENNKLFPMASLQKLMTGIIIQR
                     LIDQDVLSEDDRLSQFFPQVKGSNSITIHQLLTHTSGLREKGVKVSPYLKNEREQLQF
                     CLKHYNFVNKKSWYYSNINFSFLTGIATQVTGRTYAELVDDVIKNPLRLDDTQSYQSV
                     VNHDLVSPMRKNGKLNKINIFNQVSTAYGAGDFFTTPLNFWVLMRSFSKGYFFPTDEY
                     TKHQNDAISHYYGGLYMHGRIVNSNGAFFQYSSFGYADLKTHDTMVLFMNNKVYSDAS
                     HVAPKAFEYYQKFMFLNKIFHLVFYFVFSFFLMLLIRHSFRKRRYKKRL"
     gene            complement(5248..5517)
                     /locus_tag="VBC74_001696"
     CDS             complement(5248..5517)
                     /locus_tag="VBC74_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942628.1"
                     /translation="MVSLLLLKVKQEELIEFHNVFYREAISSYLETENFEKAKVTEEN
                     YQVFLGIRTYQKSYNYQDHSENLKGNIEVAMIGVENNISDEHIFC"
BASE COUNT         2123 a         1133 c          855 g         1935 t
ORIGIN      
        1 actagtacta ccactagcat ataacacatt gaaaataccg gcgctatgag ctgaaaatcc
       61 tatcccagat actatttttt catcgtttgt tagccaatca ttatataaaa aatttgctaa
      121 catagacgca tgaacattgt ctaaacttac cttgtggtta atgacatcca tcattaactg
      181 atatgattct aaatcagttg cttgtaactt tgaaaaaaga atttcacctt ttggagtttt
      241 aactagttga caaaggtgtt ttgactgaac attaccctgt cctccatata ttattactgt
      301 tttcattatt caatcctttc cattaatagt attggcatgt tataatcaac gacactgcca
      361 tcagcacagt acttcttgat tactttgata tccccataac ttttatattc ataagaaatt
      421 cctaaggtta taacttccaa caaggtttct cccttatgat atacaccacc ttccgtaaga
      481 aactcagaat acctaatatt tccaaccagt tggactacta atagtaactt gtctttaagc
      541 cgcgttttat acaagccaca ttgattatca gaacattgtt cacacctact caaaatatta
      601 gaacgtccca tattatcacc ttcaactaat catcttcgct ctgatatcag tactgttgga
      661 aaataaaata tttctaaaca tctacttcaa acactactat ggacaagccg tgcattgcct
      721 ttcttatgtt ttcttccttt ctgtgtcgtc tgaaactctt taaaatgagg tcatgactta
      781 agtgttgaat tcacaaaatc atgagtaaaa cactgctata aacattagtt taaatttaaa
      841 ataaacattg taaaaattaa ttataaattc acactctaat taaaatatca ttattatgtt
      901 aaaatagtaa caaagtattt catctttgtc aacactttca taaatgacat ctgctttttg
      961 cttacctttt atagaccaaa aaacgctctt agaaagcaca tctaagagcg tttttaactt
     1021 tattttggta aagatatggt aattattgtt ctatgaggga cattattctc aacactaata
     1081 gtccctcttc acctgatata ctccccttat agtggacagt aaaaaaacaa aaattcacta
     1141 gacactataa ggggagtata tcatcactaa caaagctaag ttagtaagcc taaaacttct
     1201 aaacactcaa aaaaattaat ggcttgcctt actaatcaag aacccgcact ctcatatact
     1261 tctactcctt tatgccaaag tattagtgat accataaaag aaatcaagga aatcaagata
     1321 accccaccaa tattaaaata gacatttcta tcttgtaaga agtaagcagc gggataataa
     1381 gctgtaaaag caaatggaat gacaaaacta atgatccaac gcaatagatt attatatatt
     1441 gcgacaggat acttagcaaa gtcattaaac atataaaaaa tatagataac agcaccagat
     1501 tgtttggtcc aaaaagcgat acttgacgta gcaatcttca aggaggtata aatcaaagtt
     1561 gcaaagggga taataaatat aaataataat acttttggca cgatccagct tatactagat
     1621 actgtggtag ataacagaat aaaaccaacc aataactccc ccaatgcatc tacttgaaag
     1681 gtttcaacta ggacatgaaa taaaggactg atcggcctgg ttaaatactt atcaaattct
     1741 ccttttcgaa taagcctttg accaagagcc cacaagttat caaagaaaag gtgatcaatt
     1801 ccttttggga ggagagaaaa accataaata aaagctattt gctgaaaagt ccatccttct
     1861 aaagaaggga tacgttgaaa aagaacattc agaaataaaa gatttagccc ttgtgttaaa
     1921 aatactccta aaacgcctac aaaaaagtct attttatact ccatgatttg cttcatgtat
     1981 tgtttaataa aaataaagtg cattcgctga tattttgtca tctcaacctc cttggatagt
     2041 gatatgcgac tgtacttttt tccatattaa ttgagataaa gctaccatta ctattagcca
     2101 aaatatttgt aaaagtaagg cttgcacaat ttgactgcca tcatatttac caataataat
     2161 cattactggt gtgtaaatta aagatgaaaa tggtaaaaat cctaaaatat cagcaacgat
     2221 tttcggaaaa aaagtaagag gaatcaagct ccccgacata aatgcgacta acgaattttt
     2281 taacaggttt gacccccata aatttttaaa aacaaatgcc gagaagccaa aacaaatatt
     2341 aaaaaagaag ttaatcaaaa aagctaaaat aatgctaata atataaaata ccactaacac
     2401 tatagcttga atcaaatcag tccccaagaa gaggcgtacc cccgtaataa caagcaaaaa
     2461 cggtacccct atacttaaaa agatgagcca tctagaacca atctccatga aaaggtagga
     2521 agcagcaaag tgaactggac gtaagagtcg cataataatt gaaccgtctt ttacttcgtc
     2581 acctatcata aatgaactat cagttcttgt taataaatta gtcacaaaac tcataataat
     2641 atagagaatc atatctgaca gctgaaaacc ttggattaag gattgacttg aggaatcgaa
     2701 aactgctttc catagataaa atgcaacaaa tgctcctatc acatcaccta tcctataaag
     2761 gatgaaatca acacgatatg tgattaatcc ttggatttct gtactaataa aaggtttata
     2821 acgtctccaa attctgttca tccttatagc tcatttctat agaagcgacg aatgatatct
     2881 tcaatatccg catctgtcat tttaaggtca cgtacagaaa aatctgctaa tgtctgctga
     2941 attatgtcag cagtttggta gcgagaacta tcatactgaa tatctaaaac taagtcatta
     3001 cgtttaatat taatctccga ctttccaatt aaactagagc tgatatgctc ttgtcctggg
     3061 cgcaaatcaa aagaaagagt tttcatcttt ccaaatgtct cttttagctg agaaactgtg
     3121 ccatcaaata tttcttgtcc tctatcaatc ataaaaatac gatgacaaag ttgttcaata
     3181 tcactgaggt catgtgtcgt tagtaaaatc gttgtctctt cttcttgatt aatttgtgtg
     3241 attgcacgtc ggatattatc tttcactgaa acgtccaagc caatggtggg ttcatctaag
     3301 aaaagaacct ttgggttatg taataaagaa gctgcgatat ccgctctcat acgctgtccg
     3361 agagacaaag ttctaacagg atcctttata aagtcattta gttccaaaac ctcatttaaa
     3421 aaagccatgc gtttgcgaaa ttctttatca ggaacgtcat agatttcttt taacactgta
     3481 taggtttctt gtagagctaa atcccaccac aactgcgtac gttgcccaaa aacgactcca
     3541 atatccttaa cataattttg tcggttttct tgtggcaatt tgccatcaat acggcaaaag
     3601 cccgaagttg gttttaaaat cccagttaac attttaattg ttgtagattt tcctgcaccg
     3661 ttcgctccaa taaatcccaa aatttgtcct ttaggcacat caaatgttaa atctttaaca
     3721 gcttcaaagg tgtgtttttc aggacgaaga aatgattgaa atgctccttt taaccctggt
     3781 gctttaacgg tttttataaa atttttctga aggtgagaaa cttcaatcat tgacatatta
     3841 atacctcttt tactcaacag tatttttata gcttaaatca gctcgacagt tagattatta
     3901 aactataata atgattaaca gctatttatt atagcacaat taaaaattca atgcaaacgt
     3961 tttcttagat ggtgttactg cttaatattg aataaaaaag aaggccaact tagccttctt
     4021 ttttattcat gtgatttaga gacgtttctt ataccttctt ttcctaaacg aatgacgtat
     4081 taaaagcatt aaaaagaaag aaaatacaaa gtaaaagact aagtggaaaa tcttattaag
     4141 gaacataaat ttttgataat attcaaatgc ttttggtgcc acgtgactag catcactata
     4201 gactttgtta ttcataaaaa gcaccattgt atcatgcgtc tttagatctg cataaccaaa
     4261 acttgaatac tggaaaaagg ccccattaga attaactata cgaccatgca tgtaaagccc
     4321 accataataa tgtgatatcg catcattttg atgtttggta tattcatccg ttgggaaaaa
     4381 gtacccttta gaaaaagacc gcatcaacac ccaaaagttc agcggagttg tgaaaaaatc
     4441 tccagctcca tatgctgtgg atacttgatt aaagatatta attttattta acttgccgtt
     4501 ctttcgcatt gggctaacta agtcatgatt aacaactgat tggtaacttt gggtatcatc
     4561 taatcgcaaa ggatttttta tcacatcatc aacaagttca gcgtaggtcc ttcccgtaac
     4621 ttgagtggct atacctgtta gaaaactaaa gttgatatta gaataatacc acgatttctt
     4681 attaacaaag ttatagtgtt ttaagcaaaa ttgtaattgt tctctctcat tttttagata
     4741 aggagacacc ttaacacctt tttctcttaa cccactcgta tgggtcaaca attggtggat
     4801 ggtaatacta ttactacctt ttacttgggg aaagaactga gacaatcggt cgtcttcact
     4861 caagacatct tgatcaatta atctttggat aataattcct gtcatgagtt tttggagaga
     4921 agccattgga aataatttat tattttcaat atccgtttca acttccccac ggttaatccc
     4981 atgcgagatg gtaatcggtt taccagtatt atcgctaaca atcatcattc cattaacatt
     5041 attttttcgc ataaagcgat gcaataaaat cggtgtaata ttccctttat tttctggaat
     5101 aaaaaagaaa catgccactg aaataccggt ggcgattact gctataagaa agagagatag
     5161 gagcgatttt aaaaatttac gtaccataaa taacctcctt aatatcataa cacagggcta
     5221 aattatttca aagacatttt atatattcta gcaaaaaata tgttcgtcgc taatattatt
     5281 ttcaactcct atcattgcta cctcaatatt tccttttaga ttctcactat gatcttgata
     5341 gttataactc ttctgataag tacggattcc taaaaaaact tggtaattct cttcagtcac
     5401 tttagccttc tcaaaatttt ctgtttcaag ataagaagat atcgcttctc tataaaaaac
     5461 attatgaaac tctatcaact cctcctgctt tactttcaag agaagcaagc tcaccatttc
     5521 tttaccctgt ttaaaacact ttctctagtg tcactccaat cttttaaact gtttttagga
     5581 cattttttaa agacagagat aaggttatca atgtccttga atcgccttat tcaaacacca
     5641 aacttgagca accaataatc tcatcaaaat catcaagcac aaacatttag ctttagaaac
     5701 tttgaatatt ttaaaaagag aattcatatc actttgaata tcaaaaagga gaaaaccaat
     5761 ttggttttct ccagaatata atttttcatc aacccactac agttgacaaa gagccaaaaa
     5821 acaaggaatc aatcaagaaa ttttgattga ttcctcgttt tatgcttata tgagatactt
     5881 taaacaaatt ttttcaatga gatccaacta tctagtgact cctcgatatc cttttatatc
     5941 tctcacgata gctttgagga gtaagtacta ttccattcgt tttacctata atttcaccaa
     6001 atgcatacat accatatcct atataacggc tatcgtttta agaaca
//
LOCUS       DATROD010000103         5996 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.105, whole genome shotgun sequence.
ACCESSION   DATROD010000103 DATROD010000000
VERSION     DATROD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.105"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..640
                     /locus_tag="VBC74_001697"
     CDS             <1..640
                     /locus_tag="VBC74_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HEO6942629.1"
                     /translation="KNYHLIEDGYNCFQAKLFLGGSVVKRVIKTYLFKKYVPYGFSKY
                     CLSIEVNSLVGLPHDIRSKKYKELPRKKLFDSLNKEQKSLIFKIFKTKPLTITPKSVL
                     LLTQPLAQDKCYKTPTERFQSIQEQYDYFDDIVQEYRTLGYNVYLKVHPRDVVDYSKL
                     PVELLPSNVPMEIIELMSTGRFECGITHSSTALDFLTCVDKKITLVDLKDIK"
     gene            637..2037
                     /locus_tag="VBC74_001698"
     CDS             637..2037
                     /locus_tag="VBC74_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEO6942630.1"
                     /translation="MNTKKLLQSGFIYTLGNLLVQGLAFITLPIYTRVISTEVYGQYS
                     LYVAWMNIIMLFIGLQTSGSLSSARVKYGEEFKSYSGSAFSVGNIWFLIILLIAFLFR
                     SFLAPLVGFSESIFLLMVCQSYASYVVTFFGQYFIQQQRSLANLILSLANAVSSVALS
                     LFLIFHWSDDFLSRVFGAFVPTIITGIVAFAYIYYHSKSFYNPKYFRFIVTVSVPLIF
                     HLLGHQLLGQLDRIMLARLYNTKEVAMYSFGYSLGMIIQIVLNSINMAWIPWFFDARK
                     EKLLQLSTYISRYLYLGVFLTLGYLTVFPELAQIMGGDKYSSSVQFISLIIVSYFLVF
                     LYTFPVNIQFFYANTTWIPIGTLLAAGVNWLLNLVLIPHYAAYGAAMATIISYLALLI
                     FHHIVSKVKYHYSDVSVRQYIILSGIVFSYAMLMNMFLDNIVIRWSLGIIILIVYSIV
                     FQKVILDLLSKKRRRR"
     gene            2037..3062
                     /gene="neuB"
                     /locus_tag="VBC74_001699"
     CDS             2037..3062
                     /gene="neuB"
                     /locus_tag="VBC74_001699"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEO6942631.1"
                     /translation="MVYIIAEIGCNHNGDINLAKKMVDVAVSCGVDAVKFQTFKAEKL
                     ISKFAPKAEYQKATTGTADSQLEMTKRLELSFEEYLEMRDYAISKGVETFSTPFDEES
                     LEFLISTDMPIYKIPSGEITNLPYLEKIGKQQKKVILSTGMAVMEEIHQAVNILRQNG
                     TTDISILHCTTEYPTPYPSLNLNVIHTLKDEFKDLTIGYSDHSIGSEVPIAAAAMGAE
                     VIEKHFTLDTNMEGPDHKASATPDILAALVKGVRIVEQALGRFEKIPDPVEEKNKIVA
                     RKSVVALKPIKKGDIYSIENITVKRPGNGISPMNWYDILGQEAQDDFEEDEVIRDSRF
                     ENQLPEL"
     gene            3139..4293
                     /gene="neuC"
                     /locus_tag="VBC74_001700"
     CDS             3139..4293
                     /gene="neuC"
                     /locus_tag="VBC74_001700"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEO6942632.1"
                     /translation="MKKICLVTGSRAEYGIMKPLIQRLSKDKEVNLQIIATAMHLEEK
                     YGYTYRQIEEDGFDIAYKVPLHLYDTDRRTVSTAMAHLQLGLTKIFDKEDYDLVIILG
                     DRYEMLPVVNVALIYNVPVCHLHGGETSLGNFDEYIRHAITKMSHLHLVSTEDFRQRV
                     IQMGEQPQFVINTGALGVENALSIPSLTKEAIEKQLGIVLEESYFVVLYHPVTFEQGK
                     SAGEQMKAVLSALSKFGVQCLFIGSNSDTGSDDIAKAINTYLINHENSYCFASLSTQL
                     YHSLIRHSLGLIGNSSSGLIEVPSLMKPTLNIGDRQKGRLHGESVVSVPVETPSVLEG
                     LSKLNEVTNFDNPYYKENASSIAYEAIKLYLKDEPSISQPFYDLKENNLK"
     gene            4290..4919
                     /locus_tag="VBC74_001701"
     CDS             4290..4919
                     /locus_tag="VBC74_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEO6942633.1"
                     /translation="MKKKLAFLGAGSHADAIAPVIDPLVYDFVGFFDDKDITEHDGYP
                     VLGKLYDVLPYLEDGSIDAVFITIGDNAKRKELFEYVAKDYYDFIINIISPNALVLTP
                     DSICGRGIFIGFGAFIGSKVKLFDNNVVNTGALIEHHTVVESHCNIAPNATINGLCYI
                     REEVYVGSASVIIQTLDISSCTTIGAGAVVVKDIIEPGTYVGVPAKKIK"
     gene            4930..>5996
                     /gene="neuA"
                     /locus_tag="VBC74_001702"
     CDS             4930..>5996
                     /gene="neuA"
                     /locus_tag="VBC74_001702"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEO6942634.1"
                     /translation="MKPICIIPARSGSKGLPDKNMLFLAGKPMIFHTIDAAIESGMFD
                     KKDIFVSTDSELYREICLERGISVVMRKPELSTDQATSYDMLKDFLSDYEDNQEFVLL
                     QVTSPLRKSWHIKEAMEYYSSHDVDNVVSFSEVEKHPGLFTTLSDKGYAIDMVGADKG
                     YRRQDLQPLYYPNGAIFISNKETYLREKSFFTSRTYAYQMAKEFSLDVDTRDDFIHVI
                     GHLFFDYAIREKENKVFYKEGYSRLFNREASKIILGDSKTISISLENYHNYSQGGVTL
                     ATMLENLPNFLTANVTEAFVSIGVNDLITGHSVEEIFSNFQKLYSLLAENKIKMRLTT
                     IAYTLFRETVNNADIEKINQWL"
BASE COUNT         1936 a          907 c         1125 g         2028 t
ORIGIN      
        1 taaaaattat catctaattg aagatggtta taactgtttt caagccaagt tattcttggg
       61 tggttctgtc gtaaaacgag taattaaaac ttacttattt aaaaagtatg ttccgtatgg
      121 tttttcaaaa tattgtctct caattgaggt taattcttta gtggggttac ctcatgacat
      181 aaggagtaag aaatataaag aactaccgag aaaaaaatta tttgatagtc ttaacaaaga
      241 acaaaaatca ctgattttca aaatatttaa aacaaaacca ttaactataa ctccaaagtc
      301 agtattattg ttgacacagc cacttgcaca agataaatgt tataaaacac ctacagagag
      361 gtttcaaagt attcaagagc aatacgatta ttttgacgat attgtccagg aatatagaac
      421 gttagggtac aatgtttatt taaaagttca tcctagagat gtagtagatt attccaaatt
      481 gccggtagag ctattaccat caaatgttcc tatggaaatt atagagttga tgtcaacagg
      541 tcggttcgaa tgtgggataa cacattcgtc cactgcgctg gattttttaa cttgtgttga
      601 taaaaaaata actttagtag atctgaagga tattaaatga atactaagaa attacttcaa
      661 tctggtttta tttatacact cgggaattta ttggtgcaag gattagcctt tatcacactt
      721 cccatctata ctcgggttat ctcaacagaa gtttatggcc aatatagttt atatgtcgct
      781 tggatgaata ttattatgct ttttattggc cttcagacga gcggttcttt aagttcagcc
      841 cgggtcaaat acggagaaga atttaaaagc tattcaggga gtgccttttc tgtaggtaat
      901 atatggtttc ttattatact tttgatagct tttctattta gaagttttct tgcaccatta
      961 gttggttttt ctgaatctat ttttttatta atggtgtgtc aaagttacgc tagctatgtg
     1021 gtgactttct ttggtcagta ttttatacaa caacagagga gtttggctaa tttaatatta
     1081 tccttagcca atgcagtttc atctgttgca ctatctctat ttttaatttt tcattggtcc
     1141 gatgactttt tatctagggt ttttggagct tttgttccta ctataataac tggaatagtt
     1201 gcctttgctt atatttatta tcatagcaaa tctttttaca atcctaagta ttttcggttc
     1261 attgtcactg tgtctgttcc cttgattttc catttgttag gacaccagtt gttggggcaa
     1321 ttagatagaa ttatgctagc tcgtttatac aatactaaag aagttgcaat gtatagtttc
     1381 ggttattcgc ttggaatgat tattcagatt gttttgaata gtattaatat ggcttggatt
     1441 ccatggtttt ttgatgctag aaaagaaaaa ttactgcaac tttcaactta tattagccgt
     1501 tatctctact taggagtttt cttaacatta ggttatttga ctgtttttcc tgagttagct
     1561 caaataatgg gaggagacaa gtatagtagt agtgttcaat ttatttcttt aattattgtc
     1621 agctactttt tagtttttct ctatacattt cctgtaaata tccaattctt ctatgctaat
     1681 acgacatgga ttcctattgg gacattatta gctgctggtg taaattggtt actaaacctt
     1741 gtattaattc ctcattatgc agcctatggc gctgctatgg ctacaataat atcttattta
     1801 gccttgttga tttttcatca tattgtttcg aaagttaaat accattattc agatgtctca
     1861 gttaggcagt acatcatttt aagtggtata gtatttagtt atgctatgtt aatgaacatg
     1921 ttcctagata atatcgttat tagatggagt ttaggaataa ttattcttat agtatatagc
     1981 attgtttttc aaaaagtcat tttagactta ttgagtaaaa aaagaaggag aagataatgg
     2041 tttatattat tgcagagatt ggttgcaatc ataatggaga tattaatctt gcgaaaaaaa
     2101 tggtagatgt tgccgtgtct tgtggtgttg atgctgttaa atttcagact tttaaagctg
     2161 agaaacttat ttctaaattt gctcccaaag ctgaatatca aaaagcaact acaggaacag
     2221 cagacagtca acttgagatg acgaaacgtt tagagttaag ctttgaagaa tacttagaaa
     2281 tgcgtgatta tgcaatttca aaaggtgtgg agaccttttc aacacctttt gatgaagagt
     2341 cattagagtt cttaatttct acagatatgc caatttacaa aattccatca ggagaaatca
     2401 ctaatttacc ttacttagaa aagattggca agcaacaaaa gaaagttatt ctttcgacgg
     2461 gtatggcggt aatggaagag atccatcaag cggtgaatat tttacgtcag aatggtacaa
     2521 ccgacatttc tattttacat tgtacaacag agtacccaac accttacccc tctctaaatt
     2581 taaacgttat tcatactttg aaagatgaat ttaaagattt aacgataggt tattcggatc
     2641 attcaattgg atcagaagta cctatcgcag cagcagcaat gggtgcagaa gttattgaaa
     2701 aacactttac tttagatact aatatggaag gtccggatca taaagccagt gcaacacctg
     2761 atattttagc tgctttagtt aaaggggttc gcattgttga acaagcctta ggtagatttg
     2821 aaaaaatccc agatccagta gaagagaaaa ataagattgt tgctcgtaaa tcagtcgttg
     2881 ctttaaaacc aattaaaaaa ggcgatattt attcaataga aaatattacg gtgaagcgcc
     2941 caggtaatgg tatttctcct atgaactggt atgacatctt gggacaagaa gcgcaagatg
     3001 atttcgaaga ggatgaagtt attcgtgatt cacgctttga aaatcaattg cccgagttat
     3061 aataacacct aattataatc ggcaacagat gtattacacg gctaagatta tgtaattact
     3121 atatatagaa gggatacgat gaaaaaaatt tgtttagtaa ccggatcgcg tgctgaatac
     3181 ggaataatga agccattgat tcaaagatta tcaaaagata aagaagtcaa cttacaaatt
     3241 attgcaacag caatgcatct ggaagaaaag tacggctata cttatcgtca aattgaagaa
     3301 gacggttttg atattgctta taaagttccc ttacatcttt atgatactga cagaagaact
     3361 gtatctactg caatggcgca tttacaacta ggattgacca aaatttttga caaggaagac
     3421 tatgatctag tcatcatttt aggggatcgt tacgaaatgt taccagttgt gaatgtagcg
     3481 ttgatttata atgtcccagt atgccacctt catggagggg agacctcatt aggcaatttt
     3541 gatgagtata ttcgccatgc aattactaag atgagtcacc tacacttagt ctctacagag
     3601 gactttcgtc aacgtgtgat tcagatggga gaacaacctc aatttgtaat taacacagga
     3661 gctctcggag tggaaaatgc tctatcaatc ccttctctaa ccaaagaagc aatagaaaag
     3721 caattaggta ttgtactaga ggaatcctat tttgttgttc tctatcatcc tgttactttt
     3781 gaacaaggaa aaagtgctgg agaacagatg aaagcggtac tatcagccct ttctaaattt
     3841 ggtgtccagt gtctatttat aggatccaat tcagatactg gttctgatga tattgcaaag
     3901 gctatcaata cttatttgat aaatcatgaa aatagttatt gctttgcttc tttatcaacc
     3961 caactttatc attccttaat tcgtcattca ctaggactga ttggtaattc gtcttctggt
     4021 ttaatagaag ttccttcttt gatgaaacct actcttaata ttggtgatcg ccaaaaaggt
     4081 aggctacatg gagaaagtgt agtaagtgta ccagtcgaaa ccccctctgt attggaggga
     4141 ttatcaaaat tgaatgaagt gaccaacttt gacaatccct actacaaaga gaatgcttca
     4201 tctattgcat atgaagcaat aaagctttat ttgaaagatg aaccttcaat ttcccaacca
     4261 ttttatgatt taaaggagaa taatctaaaa tgaaaaaaaa gttggcattt ttaggcgcag
     4321 gaagtcatgc tgatgcgatt gctccggtta ttgatcctct tgtgtatgat ttcgtaggtt
     4381 tttttgatga taaagatatt acggagcatg atggttatcc tgttcttgga aaactttatg
     4441 atgtgctacc ttaccttgaa gatggctcaa tagatgcagt atttattaca ataggtgata
     4501 atgctaaaag gaaagaacta tttgaatatg tagcaaagga ttattatgac tttattatta
     4561 acatcattag tcccaatgct ttagtattga caccagatag tatttgtggg cgtggtatct
     4621 ttattggttt tggggctttt ataggttcta aagtgaagct gtttgataac aacgttgtta
     4681 atacaggagc gctcattgaa catcatactg ttgtagaatc acactgtaat atagcaccta
     4741 atgctaccat aaatggtctt tgttatatta gagaagaagt ttatgtaggt agtgccagtg
     4801 ttattattca aaccttggat atttcatcgt gtacgacaat tggcgcagga gctgttgttg
     4861 taaaagatat tatagaacct ggaacctatg taggggttcc agccaaaaaa ataaaataaa
     4921 ggatatgtta tgaagccaat ttgtattatt cctgcgcgat cagggtcaaa aggattacca
     4981 gataagaata tgttattcct agcaggtaaa cccatgattt tccatactat agatgctgca
     5041 attgaatctg gaatgtttga taagaaagat atttttgtta gtacagattc agaattgtac
     5101 agagagattt gtttagaacg cggtatttca gtggtgatga gaaaaccgga actttcaact
     5161 gatcaggcaa cttcgtatga tatgttaaaa gattttttat ctgactatga agataatcag
     5221 gagtttgtgt tacttcaagt aacctctcca ctaagaaaat catggcatat aaaggaagca
     5281 atggagtatt attcttcaca tgatgttgac aatgttgtaa gtttttctga agttgagaaa
     5341 caccctggtc tgtttacgac attgtctgat aaaggctatg ctatagatat ggtgggagca
     5401 gataaaggtt atcgtcgcca agatttacaa cctttatact atccgaacgg cgctattttt
     5461 atttctaata aagaaactta cttaagggaa aaaagctttt tcacctctag gacatatgct
     5521 tatcaaatgg caaaggaatt ttcattagat gttgatacga gagatgattt tatccacgtc
     5581 atcggtcacc tctttttcga ttatgccatt agggaaaaag agaataaagt tttttataaa
     5641 gaaggctata gtcgtttgtt caatagagaa gcttcaaaga taattttagg tgattcaaaa
     5701 acgatatcta tctcactaga aaattaccat aattattccc aaggtggtgt aacattagca
     5761 acgatgttgg aaaacttacc taactttttg acagctaatg taacagaggc ttttgtttct
     5821 ataggggtaa atgatctcat tacaggtcat agtgtggaag aaatattcag caatttccaa
     5881 aaactctact cattattagc tgagaataaa ataaagatga gattgacaac tattgcttac
     5941 acccttttta gagaaactgt caataatgca gatattgaga aaatcaatca atggct
//
LOCUS       DATROD010000104         5995 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.106, whole genome shotgun sequence.
ACCESSION   DATROD010000104 DATROD010000000
VERSION     DATROD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.106"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..787
                     /locus_tag="VBC74_001703"
     CDS             <1..787
                     /locus_tag="VBC74_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6942635.1"
                     /translation="ADFLTYIIIELIISLDFWKLEWDSDIVQLIILLATSISTVMLFM
                     WVKFKEKRPISSLGLFKEGALTELSRGWLVGSVLFGACLILTIISGSGVIETVRFTAD
                     KLIWFLIFAVGWQVQSASVEILCRGWLLPVSATKYSKIVSVSISSIFFGLLHSANNHV
                     SLISIFNLCLFGLFLSLYVILKGNIWGACGIHGAWNCVQGSVFGIEVSGEPMLSNSLV
                     HVKTYGADWISGGKFGVEGSMITSIVLIVACYWLYQKSNFPYD"
     gene            894..1484
                     /locus_tag="VBC74_001704"
     CDS             894..1484
                     /locus_tag="VBC74_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEO6942636.1"
                     /translation="MPQNPTSNPEKKTYLLSELVDCFKGKAVPSKAEAGDIRIINLSD
                     MSPLGIDYHNLRTFQDEQRSLLKYLLQEGDVLIASKGTVKKVAIFEEQDYPVVASANI
                     TILRPTQHIRGYYLKLFFDSEEGQQALENANKGKAVMNISTKELLNIAIPSIPLFRQD
                     YLIQRYKQGLNDYKRKIARAEQEWERIQNDIRQQLS"
     gene            1518..2789
                     /locus_tag="VBC74_001705"
     CDS             1518..2789
                     /locus_tag="VBC74_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEO6942637.1"
                     /translation="MNEIKCPHCGTAFAINESEYHQLLEQIRGDAFDKEVSERLEKER
                     LILGEQAKNQLQEVVVEKDKEIAKLQYKVKQFLIEKDNLLKDNEYQLAEQLNQKDMML
                     RDLENQIDRLRLEHENSLQEALTKVERERDAIQNQLHIQEKEKDLALASVKSDYEVQL
                     KAANEQVEFYKNFKAQQSTKAVGESLEHYAETEFNKVRHLAFPNAYFEKDNTLSSRGS
                     KGDFIYREKDENDLEFLSIMFEMKNESDDTIKKHKNEDFFKELDKDRREKSCEYAVLV
                     TMLEADNDYYNTGIVDVSHKYPKMYVIRPQFFIQLIGILRNAALNTLKYKQELALMKE
                     QNIDITHFEEDLDIFKNAFAKNYNSASKNFQKAIDEIDKSIKRMEAVKAALTTSENQL
                     RLANNKLDDVSVKKLTRKNPTMKAKFDALKD"
     gene            2802..3233
                     /locus_tag="VBC74_001706"
     CDS             2802..3233
                     /locus_tag="VBC74_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6942638.1"
                     /translation="MKIRTATLDDSEKLVPLYQELGYAISLSEIQSILKVILTHSDYG
                     FLIAEDNGKLLAFVGYHKLYFFEKSGTYYRILALVVNEKHRRKGIASQLINHVKQLAK
                     TDGSEVLALNSSLKEYRQEAYHFYENLGFKKVSTGFSYYLD"
     gene            3325..4209
                     /gene="truB"
                     /locus_tag="VBC74_001707"
     CDS             3325..4209
                     /gene="truB"
                     /locus_tag="VBC74_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001876923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEO6942639.1"
                     /translation="MITGIINLKKEAGMTSHDAVFKLRKILHTKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTESGKIYEGEITLGYATSTEDSSGEVISRTPLTQSDLSEDVV
                     DHAMKSFTGPITQVPPMYSAVKVNGKKLYEYARSGEEVERPKRQITISEFRRTSPLYF
                     EKGICRFSFYVSCSKGTYVRTLAVDLGIKLGYASHMSFLKRTSSAGLSITQSLTLEEI
                     NEKYKQEDFSFLLPIEYGVLDLPKVNLTEEDKVEISYGRRILLENEADTLAAFYENRV
                     IAILEKRGNEFKPHKVLL"
     gene            4222..5154
                     /locus_tag="VBC74_001708"
     CDS             4222..5154
                     /locus_tag="VBC74_001708"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEO6942640.1"
                     /translation="MKIHYINDYKDIQAKEDCVLVLGYFDGLHLGHKALFDKAKKIAT
                     EKNLKIVVLTFNETPRLTFARFQPELLLHLTSPEKRSEKFQEYGVDELYLMNFTSHFS
                     KVSSDLFIKKYIYGLRAKAAVVGFDYKFGHNRTSGDYLARNFKGPVYIIDEISEGGEK
                     ISSTRIRQLITEGNVEKANQLLGYEFSTCGMVVHGDARGRTIGFPTANLAPINRTYLP
                     ADGVYISNVLINGKYYRAMTSIGKNITFGGTELRLEANIFDFDGDIYGETIEIFWLKR
                     IREMVKFNGIDDLVKQLKKDKEIALNWKKDSQTL"
     gene            5197..5610
                     /locus_tag="VBC74_001709"
     CDS             5197..5610
                     /locus_tag="VBC74_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEO6942641.1"
                     /translation="MITLFLSPSCTSCRKARAWLSKHEVAFEEHNIITSPLNKEELLQ
                     ILSFTENGTEDIISTRSKVFQKLAIDVDELSTSSLMELISENPSLLRRPIILDKKRMQ
                     IGFNEDEIRAFLPRDYRKQELKQATIRAEIEGKHD"
     gene            5603..5884
                     /locus_tag="VBC74_001710"
     CDS             5603..5884
                     /locus_tag="VBC74_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEO6942642.1"
                     /translation="MISSNYSYPLDPSWNTEDITKVLRFLNQVEHAYENSVKVDDLLD
                     SYKEFKKFVKSKAQEKQIDREFQRTSGYSTYQAVKAAQQQAKGFISLGR"
     gene            5874..>5995
                     /locus_tag="VBC74_001711"
     CDS             5874..>5995
                     /locus_tag="VBC74_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEO6942643.1"
                     /translation="MDAKFDFAKQLVYKAGQFIKSEMQNTFDVEEKSRFDDLVT"
BASE COUNT         2107 a          940 c         1120 g         1828 t
ORIGIN      
        1 agcagatttc cttacctata ttattattga attgattatc tcgcttgatt tttggaaact
       61 agagtgggat agcgatatcg tccagttaat cattttacta gcaacttcaa ttagtacggt
      121 aatgttattt atgtgggtga aatttaaaga gaagcgtcca attagttctc tagggctatt
      181 taaagaagga gccctaacag aattatctag aggatggtta gtcgggagcg ttctctttgg
      241 agcctgctta atattaacaa taatttcagg ctctggagtt atcgaaactg ttcgttttac
      301 agctgataaa ttgatttggt ttcttatttt tgctgttgga tggcaggtac aaagcgcatc
      361 tgttgaaata ttgtgtcgtg gatggttact tccggtgagt gccacaaagt atagtaagat
      421 agtttctgtc agtatcagtt caatcttttt cggtcttctg catagcgcta ataatcatgt
      481 aagccttatc tcgattttca atctgtgtct ctttggacta tttctatcgc tctatgttat
      541 tttaaaaggt aacatctggg gggcgtgtgg tattcatggc gcctggaact gtgtacaagg
      601 gagtgtcttt ggaatcgaag ttagtggaga gccaatgttg tctaactccc tagtccatgt
      661 taaaacctat ggtgctgact ggattagcgg tggaaaattt ggtgtggaag gaagcatgat
      721 taccagtatt gttcttatcg tagcatgtta ttggctttat caaaaatcga attttcctta
      781 cgactaatag atttaatcta aataagcttc tcttactgaa agcttatttt ttattttagt
      841 attaaaaaac tatgttttgt gatataatat tttaaagacg aaaggagtct attgtgccac
      901 aaaatccaac aagtaaccca gagaaaaaaa cttatttatt atctgaattg gtagactgct
      961 ttaaaggtaa agctgtgcct tcaaaagcag aagcaggtga tatccgtatt attaatttga
     1021 gtgatatgtc tcctttagga attgactacc ataacttaag aacattccaa gatgaacaac
     1081 gatcgctttt aaaatatcta cttcaggaag gcgatgtttt aatcgcttca aaaggaactg
     1141 tcaagaaagt tgctattttt gaagagcaag attaccctgt cgttgcctct gctaatatta
     1201 caatactaag gcctacacaa cacattagag ggtactactt aaagcttttt tttgattcag
     1261 aagaaggtca acaggcactt gagaatgcta acaaaggaaa agctgtgatg aatattagca
     1321 caaaggaact gttaaatatt gcaataccaa gtatcccgct ttttagacag gattatctta
     1381 tccaacgtta taaacagggg ctcaacgatt acaaacgtaa gatagcacgt gcagagcagg
     1441 agtgggaacg tatccaaaat gacatcagac aacaacttag ctagaaccaa atacaaaaca
     1501 tcataggaag agttatcatg aacgaaatca aatgccctca ttgtggaaca gcttttgcca
     1561 tcaacgagtc tgaataccat caattactag aacaaattcg tggagatgct tttgacaaag
     1621 aagtaagtga acggttggaa aaagaacgtc taatattagg ggagcaagca aaaaatcaat
     1681 tacaggaagt tgttgtagaa aaagacaagg agatagctaa acttcagtac aaagtcaaac
     1741 aatttcttat agaaaaagac aatcttctca aagacaatga gtaccaactc gctgagcaat
     1801 taaatcaaaa agacatgatg cttcgcgacc ttgaaaacca aatcgataga ctacgtttag
     1861 agcatgaaaa tagcttgcaa gaggcgctaa caaaagtcga acgagaaaga gatgcaatac
     1921 aaaatcagtt gcacattcaa gaaaaagaaa aagatttagc tttagcttca gtaaaaagtg
     1981 attatgaagt acaactaaag gcagccaatg aacaagtaga attctataaa aacttcaaag
     2041 ctcaacagtc tactaaagca gtaggagaaa gtttagaaca ttatgctgaa acagaattta
     2101 ataaagtgcg acatttggcc tttcctaatg cttattttga gaaggacaat acattatcaa
     2161 gtcgtggctc aaagggggac tttatctatc gagaaaagga tgaaaatgac cttgagtttt
     2221 taagtatcat gtttgaaatg aaaaatgagt ctgatgatac tatcaagaag cataaaaatg
     2281 aagatttttt caaagaatta gataaagatc gtcgtgaaaa atcttgcgaa tacgcagttt
     2341 tagtaactat gcttgaagca gacaatgact attataatac tggaattgtt gatgttagtc
     2401 acaaataccc taaaatgtac gttatacgtc cacaattttt tatccaatta attggtattc
     2461 taagaaatgc agcactcaat accttaaaat ataaacaaga gcttgctttg atgaaagaac
     2521 aaaatattga catcacacat tttgaagaag atttagatat tttcaaaaat gcatttgcta
     2581 aaaattataa ttctgcaagc aaaaatttcc agaaagcaat cgatgaaata gataaatcta
     2641 ttaaacgtat ggaagctgtt aaggctgctt taacaacgtc tgaaaatcaa ctacgtcttg
     2701 caaataataa attagacgat gtttctgtca agaaattaac aagaaaaaat ccaacaatga
     2761 aagcaaaatt cgatgctcta aaagactaag aggttatgat tatgaagatt agaactgcta
     2821 cactagacga tagtgaaaaa ttagtccctt tgtatcaaga attaggatac gcaatatcat
     2881 tatcagagat tcaatctatt ttaaaggtta ttcttactca ttcagattat ggctttttga
     2941 tagctgagga taatggcaaa ctactcgcct tcgttggata tcataaactt tacttttttg
     3001 aaaaaagtgg gacttactat cgtattttag cactagtagt taatgaaaag caccgtcgta
     3061 aaggcatcgc tagtcaactt attaatcacg ttaaacagct agctaaaaca gatggaagtg
     3121 aagtactggc tttaaatagc agtttaaaag aatatcgtca ggaagcttat catttttatg
     3181 aaaacctagg gttcaagaag gttagtacag gcttttcata ctatttagat taggtataaa
     3241 tccttatagc actataccaa aagagcgtaa gtttttacgc tcctttcttt gtgttaccgc
     3301 ttatttactg atataatata aaacatgatt acaggaataa ttaatttaaa gaaagaagct
     3361 ggaatgacat cacacgatgc tgtattcaag cttcgaaaga tattacatac aaaaaaaata
     3421 ggacacggcg gaactcttga tccagatgtt gttggggttt taccaattgc ggttggaaaa
     3481 gccactcgtg ttattgaata tatgacagaa agtggcaaaa tttatgaagg agaaatcact
     3541 ttaggatatg ccacaagtac agaagattct agtggagagg tcatttcaag aactccacta
     3601 actcaatccg atcttagcga ggatgttgtg gatcatgcga tgaaaagttt taccggtcca
     3661 attacacaag ttcctcctat gtattcagca gttaaagtaa atggtaaaaa actctatgaa
     3721 tatgctcgtt caggagaaga agtagagcga cctaaacgac aaataacaat ttctgaattt
     3781 agacgcacaa gcccattgta cttcgaaaaa ggcatctgcc gttttagttt ctatgtctca
     3841 tgcagtaaag gtacctatgt taggacgtta gctgttgatc taggaataaa actaggatat
     3901 gctagccata tgtctttcct aaaaagaacc tcatctgcag gattatctat tacacaatca
     3961 ttaacgcttg aagaaattaa tgaaaaatat aagcaagaag atttttcatt tttacttcca
     4021 attgaatatg gtgttttaga tctcccaaaa gttaacctaa ctgaggaaga taaagttgaa
     4081 atctcatacg gacgtcgtat cttattagaa aatgaagcag ataccttagc agctttttat
     4141 gaaaatagag ttatcgctat actagaaaaa cgtggcaatg aatttaaacc acacaaagta
     4201 ttgttataaa gaaagtcaat catgaaaatc cattacatta atgattataa agatattcaa
     4261 gctaaagaag attgcgtctt agttttgggc tattttgatg gcttacatct gggacataaa
     4321 gccctctttg ataaagctaa aaaaatcgcg accgaaaaaa atttgaaaat tgtcgtctta
     4381 acgtttaatg agacacctcg tctaaccttc gctcgcttcc aacctgaatt attgctacat
     4441 cttacctctc cagaaaaacg ttctgaaaaa tttcaagagt atggcgtcga tgagttatat
     4501 ttaatgaatt ttaccagtca tttttcaaaa gtatcttctg acctttttat taagaaatat
     4561 atttatggtc tcagagcaaa agctgctgtt gtcggttttg attataagtt cggtcataat
     4621 cgtaccagtg gtgattatct agcgcgtaat ttcaagggac cagtctatat tattgatgaa
     4681 atttctgaag gtggtgaaaa aatctcttct acacgtattc gtcaattgat tacagagggt
     4741 aatgttgaga aagctaacca acttttgggt tatgagtttt caacctgtgg catggtagta
     4801 catggagatg ctagaggacg aactataggg ttcccaactg ctaatctagc tcctattaat
     4861 cgcacttact tacctgcaga cggtgtttat atttccaatg ttctgattaa tggaaaatat
     4921 tatagagcaa tgacgagcat tggaaaaaat atcacttttg gtggtacaga acttcgttta
     4981 gaagctaata tctttgattt tgatggcgac atttatggag aaactattga aattttctgg
     5041 ttaaaaagaa tcagagaaat ggtaaagttt aatggtattg acgatcttgt aaaacaactt
     5101 aaaaaagata aagaaattgc tttaaattgg aaaaaagata gtcaaactct ttaatttttt
     5161 gtataatagt ctctagtaaa tacaagaggc ggttatatga ttaccttatt tttatcaccc
     5221 agctgtacta gttgtcgaaa agcgcgtgct tggctaagta agcatgaggt tgcttttgaa
     5281 gagcataaca ttattacaag tccactcaat aaagaagaac tattacaaat tctatcgttt
     5341 acagaaaatg gtacagaaga cattatttcc acacgttcaa aagtctttca aaaacttgct
     5401 attgatgtag acgaattatc aacgtcttca ttaatggaat taatttcaga aaatccaagt
     5461 cttttacgac gcccaattat tttagacaaa aaacgtatgc agattggttt taatgaggat
     5521 gaaattcgtg ccttcttacc gcgtgactat cgtaaacaag aactgaaaca ggccactatt
     5581 cgtgctgaaa ttgagggaaa acatgattag tagtaattat agttatcctc ttgatccttc
     5641 atggaatacg gaagatatta ctaaggtact tcgcttttta aatcaagttg aacacgccta
     5701 tgaaaatagt gttaaagttg atgatttgct agatagctat aaagaattta aaaaatttgt
     5761 taaatccaag gcgcaggaaa aacaaattga ccgtgaattc cagagaacta gcggttattc
     5821 cacttatcaa gctgttaaag cagcacaaca acaagcgaaa ggatttattt cacttggacg
     5881 ctaaatttga tttcgctaaa caattagtgt ataaagctgg tcaattcatt aaatctgaga
     5941 tgcaaaatac ttttgatgtt gaggaaaagt ctcgatttga tgatttagtg acaag
//
LOCUS       DATROD010000105         5979 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.107, whole genome shotgun sequence.
ACCESSION   DATROD010000105 DATROD010000000
VERSION     DATROD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.107"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            45..398
                     /gene="yaaA"
                     /locus_tag="VBC74_001712"
     CDS             45..398
                     /gene="yaaA"
                     /locus_tag="VBC74_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEO6942644.1"
                     /translation="MDYKLFDEYITLQSLLKEIGIIQSGGAIKKFLADNQVLFNGDLE
                     NRRGKKLRLGDIITIPDQNIEIIIRKPSDQEIEERNVEIAEKQRVSAIVKEMNKNTNK
                     DKSKTSKKPVRFPGT"
     gene            401..1510
                     /gene="recF"
                     /locus_tag="VBC74_001713"
     CDS             401..1510
                     /gene="recF"
                     /locus_tag="VBC74_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEO6942645.1"
                     /translation="MWIKNISLKHYRNYEEAQVDFSPNLNIFIGRNAQGKTNFLEAIY
                     FLALTRSHRTRSDKELVHFKHHDVQITGEVIRKSGHLNLDIQLSEKGRITKVNHLKQA
                     KLSDYIGAMTVVLFAPEDLQLVKGAPSLRRKFLDIDIGQIKSTYLAELSNYNHVLKQR
                     NTYLKTTNNVDKTFLTVLDEQLADYGSRVIEHRFDFIQALNDEADKHHYIISTELEHL
                     SIHYKSSIEFTDKSSIREHFLNQLSKSHSRDIFKKNTSIGPHRDDITFFINDINATFG
                     SQGQQRSLILSLKLAEIELIKTVTNDYPILLLDDVMSELDNHRQLKLLEGIKENVQTF
                     ITTTSLEHLSALPDQLKIFNVSDGTISINEKKATD"
     gene            complement(1522..2388)
                     /locus_tag="VBC74_001714"
     CDS             complement(1522..2388)
                     /locus_tag="VBC74_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEO6942646.1"
                     /translation="MEGLLIALIPMFAWGSIGFVSNKIGGRPNQQTFGMTLGALLFAI
                     IVWLFKQPEMTASLWIFGILGGILWSVGQNGQFQAMKYMGVSVANPLSSGAQLVGGSL
                     VGALVFHEWTKPIQFILGLTALTLLVIGFYFSSKRDVSEQALATHQEFSKGFATIAYS
                     TVGYISYAVLFNNIMKFDAMAVILPMAVGMCLGAICFMKFRVNFEAVVVKNMITGLMW
                     GVGNVFMLLAAAKAGLAIAFSFSQLGVIISIIGGILFLGETKTKKEQKWVVMGILCFV
                     MGAILLGIVKSY"
     gene            complement(2444..3106)
                     /locus_tag="VBC74_001715"
     CDS             complement(2444..3106)
                     /locus_tag="VBC74_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6942647.1"
                     /translation="MKNYGKILKELREDKGISLSSLAKSAQLSKSTLSRFENGETQIG
                     IDKFIKALQTLEVGVTINEVSILDSKVKAGTSNTDLEQLTLLESYRDNEDIMRIFSFQ
                     EQQSCDRIESNVLKILAKLFISNLGLNMRLPQDEINLVVTYLNGVTQYNDFYFKVICY
                     FQDILPEDVILNKISNMTKEQLPYSKSLVNLLIKQVIIALEKDSVDKAIVFADLANKV
                     SQ"
     gene            complement(3182..4663)
                     /gene="guaB"
                     /locus_tag="VBC74_001716"
     CDS             complement(3182..4663)
                     /gene="guaB"
                     /locus_tag="VBC74_001716"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEO6942648.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNEVDMNTKLADNLTL
                     NIPIITAAMDTVTDSKMAIAIARAGGLGIIHKNMSIVDQAEEVRKVKRSENGVIIDPF
                     FLTPDNTVSEAEELMQNYRISGVPIVETLENRKLVGIITNRDMRFISDYKQLISEHMT
                     SQNLVTAPIGTDLETAERILHEHRIEKLPLVDDEGRLSGLITIKDIEKVIEFPKAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQITAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIVFQMLGGIRSGMGY
                     VGAANIKELHDNAQFVEMSGAGLKESHPHDVQITNEAPNYSVH"
     gene            complement(4820..5305)
                     /locus_tag="VBC74_001717"
     CDS             complement(4820..5305)
                     /locus_tag="VBC74_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEO6942649.1"
                     /translation="MNKKETRHQLIRSLVSETKVRTQHELRELLEKNGVSVTQATLSR
                     DMKELNLIKVNESSDNATETYYEIHSISQKRWEERLRFYMEDALVMLFPVQNQIVLKT
                     LPGLAQSFGSILDSILLPEILATVCGDDTCLIICNNSEEAQKCFEKLSQYTPPFFFSK
                     K"
     gene            complement(5315..>5979)
                     /locus_tag="VBC74_001718"
     CDS             complement(5315..>5979)
                     /locus_tag="VBC74_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO6942650.1"
                     /translation="YFYFRQLDDFKHFTIEQFDHIVSHIKHRTALKNHTLFFEGDYRE
                     KLFLIQSGHVKIEQSDASGSFIYTDYVRQDTVFPYGGLFLDDDYHFSAVAITDIEYFS
                     LPMALYEEYSLQNINQMKHLCRKYSKLLRVHEIRLRNMVTSSASMRVIQSLATLLLQV
                     PTERGHLPFPITTIEIANMSGTTRETVSHVLKELRQKDIVEMKGKKLLYNNKNYFKKF
                     IE"
BASE COUNT         2044 a         1155 c          905 g         1875 t
ORIGIN      
        1 tatgctataa tgatgtgata tctaaaattg gagattaaaa agtcatggat tataaattat
       61 ttgatgaata tattacttta caatcacttc taaaagagat tggtatcatt caaagtggcg
      121 gagccattaa aaaattttta gcggataatc aagtattgtt taacggtgac cttgaaaata
      181 ggcgtggaaa gaaactccgc ctgggggata ttattactat tcctgatcaa aatatcgaga
      241 tcattataag gaagccatca gatcaagaaa ttgaagaacg taatgtagaa atagctgaaa
      301 aacagcgtgt ttcagcaatc gttaaagaaa tgaataaaaa caccaacaaa gataaatcaa
      361 aaactagcaa aaaaccagtt cgctttccag ggacataaaa atgtggataa aaaatatttc
      421 tttaaaacat tatcgtaatt atgaagaagc acaagtagat ttttcaccca acctcaatat
      481 tttcattggc cgcaatgctc aaggaaagac aaatttttta gaagcaatct acttcttggc
      541 tttgactcga agtcaccgta cccgttctga taaagaactt gtacatttta aacaccatga
      601 tgtacaaatt acaggtgagg taatccgcaa aagtggccat cttaacttag atattcaatt
      661 atctgaaaaa ggaagaatca ctaaagttaa ccatcttaaa caggcaaaat tatctgatta
      721 tattggggct atgactgtcg tactttttgc ccctgaagat cttcaattag ttaaaggtgc
      781 acctagtcta agacgaaaat ttttagatat agatattgga caaattaagt caacgtactt
      841 agctgaactt tcaaattaca atcatgtatt aaaacaaaga aacacttatc taaaaactac
      901 taataacgtc gacaaaactt ttttaacagt ccttgatgaa caattagctg attatggaag
      961 tcgtgtcatt gaacaccgtt ttgattttat ccaagcccta aatgatgaag cggacaaaca
     1021 tcactacatt atcagtactg agttagaaca tttatctatt cactacaaat cgtcaataga
     1081 gtttacagat aagtcaagta ttagggaaca ttttttaaac caattatcta aaagccactc
     1141 aagagacatc tttaagaaaa acacaagcat cggacctcat cgtgatgata ttactttctt
     1201 tattaatgat atcaatgcta cttttggtag ccaagggcaa caaagaagcc ttattttatc
     1261 attgaaatta gcagaaattg agctaataaa aaccgttacc aacgattatc cgattctctt
     1321 acttgacgac gtcatgagtg aacttgacaa ccatcgtcaa ctaaaactct tagagggaat
     1381 caaagagaat gttcaaacat ttattactac aacaagttta gaacatttaa gtgctttacc
     1441 tgatcaactc aaaatcttta acgttagtga tggtactatt agtattaatg aaaaaaaagc
     1501 aacagattaa tctgttgctt tttaataaga tttaacaata ccaagtaata tagcacccat
     1561 aacaaaacaa aggataccca tgacaaccca tttctgctct ttcttcgttt ttgtctcacc
     1621 taaaaataaa ataccaccaa taatagagat aattactcca agttgagaaa aactaaaagc
     1681 aattgctagc cctgctttag ctgctgccaa taacatgaag acattaccaa cgccccacat
     1741 gagacctgta atcatatttt taacaacaac agcctcaaag ttaacacgaa acttcatgaa
     1801 acaaattgca cctagacaca ttccaacagc catgggtaaa atgacggcca tagcgtcgaa
     1861 cttcataatg ttgttaaata aaactgcgta cgagatgtaa cctacagttg aataagcaat
     1921 tgtagcaaat ccttttgaaa actcttgatg tgttgccaaa gcttgttctg aaacatcacg
     1981 tttacttgag aaatagaagc cgataactaa taatgtcaac gctgtcaatc ctaaaataaa
     2041 ttggattggc ttagtccatt catgaaagac taaagcacca actaggcttc cacctactaa
     2101 ttgtgcacca cttgacagtg gattagcaac agagactccc atatatttca ttgcttgaaa
     2161 ttgaccattt tggccgactg accataggat accacctaag ataccaaaaa tccacaatga
     2221 ggcagtcatc tctggctgtt taaataacca tacgataatc gcaaatagca atgctcctaa
     2281 agtcattcca aatgtttgtt gatttggacg ccctccaatt ttattactaa caaatccaat
     2341 acttccccac gcaaacatgg gaatcaatgc aataagtaaa ccttccaatt ctttctcctt
     2401 acaatggaat attaagtatt acctattttc aatcctagag aatctattga ctaactttat
     2461 tagcaagatc agcaaatact atagctttat caacactatc tttttccaaa gctataatga
     2521 cttgttttat caataaattg accagtgatt tagaataagg taactgttct ttagtcatat
     2581 tactaatctt atttaaaata acatcttcag gtaaaatatc ttgaaaatag cagatgactt
     2641 tgaaataaaa atcattgtac tgtgtcacac cattcagata agtaactacc aggttaatct
     2701 catcttgagg cagcctcata tttaacccta aatttgaaat aaaaagttta gccaatattt
     2761 tgagaacatt gctttctatt ctatcacagg attgttgttc ttggaaactg aaaattctca
     2821 tgatatcttc attatctcga taagattcca gtaaagttaa ttgctctaaa tcagtattag
     2881 aagttccagc cttaactttt gaatctagaa tggatacttc attgatagtt accccaacct
     2941 ccaacgtttg taaagcttta ataaatttat caatacctat ttgtgtctca ccattttcaa
     3001 aacgtgatag agttgatttt gataattgag cacttttggc tagagaactt agggatatac
     3061 ctttgtcttc tctcaactct tttaaaattt ttccataatt tttcatacaa aaactttact
     3121 atatttgttt cttttatgca acaaaaagca tcctcatagg ggatgctttt ttgtccttat
     3181 tttagtgtac tgaataattt ggtgcctcat ttgtaatttg aacatcatgg ggatggcttt
     3241 ctttcaaacc agctcctgac atttcaacaa attgagcatt atcatgaagt tcttttatgt
     3301 tcgctgcacc tacatatccc ataccagaac gaatgccacc aagcatttgg aaaacaatat
     3361 cggctactga acctttgtat gctacacgac cttcaatacc ttctggaacc aatttgttag
     3421 cctcgtttac agaaccttgg aaataacgat cgcttgaacc ttttttcatt gctgcaattg
     3481 agcccatacc acggtatgtc ttgaacttac gcccttggaa aatttctgtt tctcctggcg
     3541 cttcatctgt tcctgcaaac attgaaccta gcataactgc atttccacct gctgcaagtg
     3601 cttttacaat atcacctgaa tatttaatac caccatcagc aatgatagtc ttaccgtact
     3661 cacgcgcaac agccgctgca tcgtaaatag ctgtaatctg aggaacacct accccagcaa
     3721 cgacacgagt cgtacaaatt gaacctggac caataccaac tttaacgaca tccacacctg
     3781 catcataaag tgcacgcgct ccttctgctg tagcaatatt tcccgcaatc aatgtgcgat
     3841 tagggaagtg agcacgaatt tcagcaattt tacggagcac accagctgaa tgtccatgtg
     3901 ctgtatcaat gacaatagca tctgcacctg cttcaaacaa agcttctgca cgttcaaaag
     3961 tatcagaggt tacacctact gcacctgcaa ctaagagacg tccaaattca tcttttgctg
     4021 ccttaggaaa ttcaataact ttttcgatat ccttaatagt aataagtcct gaaagacgtc
     4081 cctcatcatc tactaaaggt agcttttcaa tacgatgctc atgaagaata cgctccgcag
     4141 tttcaagatc tgtaccaata ggagctgtta caagattttg acttgtcatg tgctctgaga
     4201 ttagttgttt ataatcagaa atgaaacgca tatcacggtt agtaataatt ccaactaatt
     4261 tacgattctc gagtgtctct acaataggaa ctccactaat gcgatagttt tgcatcaatt
     4321 cctcagcttc agagacagta ttatctggtg tcaagaagaa tgggtcaata ataacaccat
     4381 tttctgagcg tttaacttta cgaacttctt ctgcttgatc aactattgac atatttttat
     4441 gaatgatacc aagcccacca gcacgtgcaa tagcaatagc catcttacta tctgtaactg
     4501 tatccattgc agctgtaatg attggaatgt ttaatgtcaa attatcagcc aactttgtat
     4561 tcatatcaac ttcatttggt aaaacatggc tttcagcagg aataagcaga acatcatcaa
     4621 aagtaaaacc ttttttcaaa aacttagtat cccaatttga catcaaaaaa tcctcttttc
     4681 ttagatagta gatataaaaa atcccattaa aattatattg ttactatcat atcacgctaa
     4741 aatcatttgt caatataaaa agagcataga attaatagct tctagctctt taaagtaatg
     4801 aaaaaatctt cttaattttc tatttttttg agaagaagaa aggcggcgta tactgactta
     4861 atttctcaaa acacttttga gcttcttcac tattattaca aataatcaaa caagtatcat
     4921 caccacagac agttgctaag atctcaggta ataaaatcga atctaatatg gagccaaaag
     4981 attgagctaa gccaggtagt gtcttcaaaa cgatttggtt ttgaactgga aacagcatga
     5041 ctagggcatc ttccatgtaa aaacgtaacc gttcttccca acgcttctga gatatactat
     5101 gaatctcata ataagtttct gtagcattat cactgctttc gttaacctta attaaattaa
     5161 gttccttcat atctcgagat aatgttgctt gcgtcactga aacgccattt ttttctagta
     5221 attcccttaa ctcatgttgt gtacgcacct tagtctcaga gactaaggaa cgaattaatt
     5281 ggtgtcttgt ttctttttta ttcatataaa ctccttattc aatgaatttc ttgaaataat
     5341 ttttattatt atagagaagc ttctttcctt tcatttcaac gatatctttt tggcgtaatt
     5401 cctttaaaac atgactaact gtctcgcgag tagttccgct catattggca atttcaatcg
     5461 ttgtaatagg aaaaggtaaa tgtccccttt ctgtaggaac ttgcaaaagt agagttgcaa
     5521 gcgattggat aactctcata ctggcacttg aagtcaccat attacgaagt cgtatttcat
     5581 gaactcgtaa tagcttagaa tatttccgac aaagatgctt catttgattg atattttgaa
     5641 gagagtattc ttcgtataaa gccattggca gagaaaaata ctcaatatcc gtaatagcta
     5701 ctgctgaaaa atggtaatca tcatccaaaa agagacctcc ataaggaaaa actgtatctt
     5761 gtctaacata atctgtataa ataaaagaac cagaagcgtc tgattgttca atttttacat
     5821 gtcctgactg gattaaaaac agtttttcac ggtaatcacc ttcaaaaaat aaggtatgat
     5881 tttttagagc tgtacgatgt ttaatgtggc tcacaatatg gtcaaattgt tcaatagtaa
     5941 agtgtttaaa atcatctagt tgtctgaaat aaaaatatt
//
LOCUS       DATROD010000106         5861 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.108, whole genome shotgun sequence.
ACCESSION   DATROD010000106 DATROD010000000
VERSION     DATROD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.108"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            177..1538
                     /gene="dnaA"
                     /locus_tag="VBC74_001719"
     CDS             177..1538
                     /gene="dnaA"
                     /locus_tag="VBC74_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEO6942651.1"
                     /translation="MTENEQLFWNRVLELSRSQIAPAAYEFFVLEARLLKIEHQTAVI
                     TLDNIEMKKLFWEQNLGPVILTAGFEIFNAEITANYVSNDLHLQETSFSNYQQSSNEV
                     NTLPIRKIDSNLKEKYTFANFVQGDENRWAVSASIAVADSPGTTYNPLFIWGGPGLGK
                     THLLNAIGNQVLRDNPNARVLYITAENFINEFVSHIRLDSMEELKEKFRNLDLLLIDD
                     IQSLAKKTLGGTQEEFFNTFNALHTNDKQIVLTSDRNPNQLNDLEERLVTRFSWGLPV
                     NITPPDFETRVAILTNKIQEYPYDFPQDTIEYLAGEFDSNVRELEGALKNISLVADFK
                     HAKTITVDIAAEAIRARKNDGPIVTVIPIEEIQIQVGKFYGVTVKEIKATKRTQDIVL
                     ARQVAMYLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMVAQDDNLRIEIETIKN
                     KIR"
     gene            1693..2829
                     /gene="dnaN"
                     /locus_tag="VBC74_001720"
     CDS             1693..2829
                     /gene="dnaN"
                     /locus_tag="VBC74_001720"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEO6942652.1"
                     /translation="MIHFSINKNFFLHALTVTKRAISHKNAIPILSTVKIEVTRDAII
                     LTGSNGQISIENTIPASNENAGLLVTNPGSILLEAGFFINIISSLPDVTLEFTEIEQH
                     QIVLTSGKSEITLKGKDVDQYPRLQEMTTDTPLTLETKLLKSIINETAFAASQQESRP
                     ILTGVHLVISQNKYFKAVATDSHRMSQRTFQLEKSANNFDLVVPSKSLREFSAVFTDD
                     IETVEVFFSDSQMLFRSENISFYTRLLEGNYPDTDRLLTNQFETEIIFNTNALRHAME
                     RAYLISNATQNGTVRLEIQNETVSAHVNSPEVGKVNEELDTVSLKGDSLNISFNPTYL
                     IESLKAVKSETVTIRFISPVRPFTLTPGEDTEDFIQLITPVRTN"
     gene            2887..3780
                     /locus_tag="VBC74_001721"
     CDS             2887..3780
                     /locus_tag="VBC74_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEO6942653.1"
                     /translation="MMSNMTLYIIANPHAGNKNASTIVGKIQELYHTEDISVFYTEQK
                     DDEKKQVINILRSFKESDHLMIIGGDGTLSKVMTYLPQHIPCAYYPVGSGNDFARALK
                     IPNLKETLTAIQTERLKEINCFIYDKGLILNSLDLGFAAYVVWKASNSKIKNILNRYR
                     LGKITYIVIAIKSLLHSSKVQVLVEGETGQRIKLNDLYFFALANNTYFGGGITIWPKA
                     SALTAELDMVYAKGHTFLKRLSILLSLVFKRHTTSKSIKHQTFKAMTVYFPKNSLIEI
                     DGEIVELDQISLKCQKRYLYM"
     gene            3790..3987
                     /locus_tag="VBC74_001722"
     CDS             3790..3987
                     /locus_tag="VBC74_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEO6942654.1"
                     /translation="MYQVGSLIEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            4190..4306
                     /locus_tag="VBC74_001723"
     CDS             4190..4306
                     /locus_tag="VBC74_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942655.1"
                     /translation="MDNLIRLAEIFDVSLDEHVLAKTNEVKVERIYEKNLDL"
     gene            4550..5665
                     /gene="ychF"
                     /locus_tag="VBC74_001724"
     CDS             4550..5665
                     /gene="ychF"
                     /locus_tag="VBC74_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEO6942656.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEEEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ADPDDIDYVNQIRAFAETESAEVVVISARAEEEISELDDEDKLEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LIQYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            5749..>5861
                     /locus_tag="VBC74_001725"
     CDS             5749..>5861
                     /locus_tag="VBC74_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEO6942657.1"
                     /translation="MVKMIVGLGNPGSKYNDTKHNIGFMAVDRIVKKLDVN"
BASE COUNT         2038 a          887 c         1056 g         1880 t
ORIGIN      
        1 tatccacaag gtattttgct atttttctgt tgattctcta aacttttcta atttccacag
       61 tctgtggaaa actttaatta acattgttga ttttattctt caacatctgt ggaaaacttt
      121 atttttttat ggtacaatat aacaataatt atccacaaga caataaggaa gaagctatga
      181 cggaaaacga acaactattt tggaatagag tactagagct atctcgttct caaatagcac
      241 cagcagctta tgaatttttt gttctagagg ctagactcct caaaattgaa catcaaactg
      301 cagttattac tttagataac attgaaatga aaaagctatt ctgggaacaa aatttggggc
      361 ctgttatcct aacagctggt tttgaaattt tcaatgctga aattacagct aactatgtct
      421 caaacgattt acatttacaa gaaactagtt tttctaacta ccagcaatct agcaacgaag
      481 taaatacttt accaattaga aaaatcgact ctaaccttaa agagaaatat acttttgcta
      541 attttgttca aggagatgaa aatagatggg ctgtttcagc atcaattgct gtagctgata
      601 gtcctggcac gacttataat cctctattta tctggggggg acctggtcta ggaaagacgc
      661 atctactaaa tgctattgga aatcaagttt taagagataa tccaaacgcg agggttttat
      721 acatcactgc tgagaatttt attaatgaat ttgtcagtca tattcgttta gattcgatgg
      781 aagaattaaa agaaaagttt cgcaacttgg acttactcct gattgatgat attcagtcgc
      841 ttgctaagaa aaccttaggg gggacccaag aggagttctt caatactttc aatgctttac
      901 atacaaacga taaacaaatc gtattgacca gtgaccgaaa tccaaatcaa ttaaatgatc
      961 tagaagaacg tctagtcacg cgctttagtt ggggactccc agtaaatatc acaccacctg
     1021 attttgaaac acgagttgct attttaacca ataaaattca agaatatcct tatgattttc
     1081 ctcaagatac cattgaatac ttagcaggag aatttgattc caacgtacgt gaattagaag
     1141 gagccttgaa aaatattagt ctagttgctg actttaagca tgctaaaact attacagtag
     1201 atatagctgc agaagctatc agagcacgta aaaatgacgg tcctattgtt actgttattc
     1261 ctatagaaga aattcaaata caagttggta aattctatgg cgtaactgta aaagagataa
     1321 aagcaactaa aagaacacaa gatattgtcc ttgcaagaca ggtagccatg tacttagctc
     1381 gtgagatgac agataacagt ctccccaaaa taggtaaaga atttggggga cgagatcact
     1441 caactgttct ccacgcttat aataaaataa aaaatatggt tgctcaagat gacaacttac
     1501 gaattgagat agaaactatc aaaaataaaa tcaggtaggc tttgtggata agtatcagaa
     1561 aaacacgaga cttttccaca ggttgtgaac aagttatttt ccttatgaat actaaagtta
     1621 taatacttat tcacaacttt aacaggacct actattacta ctaaatttat actaatatat
     1681 aaaggatata ctatgattca tttttcaata aataaaaatt tcttcttgca tgctctaaca
     1741 gtaaccaaac gagctattag tcataaaaat gcgattccaa tcctttcaac tgttaaaata
     1801 gaagtgacta gagatgctat cattttaacg gggtcaaatg gacaaatttc aattgaaaat
     1861 actattcctg cttcaaatga aaatgcaggt ttactagtaa cgaatccagg ctctattttg
     1921 ttagaagctg gtttctttat taatattatt tcaagtttac cagatgtaac tttagaattt
     1981 acagagattg aacaacatca aattgttctt actagtggaa aatcagagat tactttgaaa
     2041 ggtaaggatg tcgatcaata ccctcgtctg caggaaatga caacagatac tccattaaca
     2101 ttagaaacta aactgttaaa atcaattatt aatgaaactg cttttgctgc tagccaacaa
     2161 gaaagccgtc caatcttaac aggtgttcat ttggttatca gtcaaaataa atactttaag
     2221 gctgttgcga cagattcaca ccgtatgagt caacgcactt tccaattaga gaaatcggct
     2281 aataattttg atttggttgt tccaagtaaa tcccttcgag aattttcggc tgtttttaca
     2341 gatgatattg aaactgtaga ggttttcttc tcagatagtc aaatgttatt tagaagtgaa
     2401 aatatcagct tctatacacg tttgcttgaa ggaaactacc ctgatactga tcgcctccta
     2461 actaatcagt ttgaaaccga aattatcttt aatacaaatg ctttacgcca tgctatggaa
     2521 cgtgcttatt taatttcgaa tgcaactcag aacggtactg ttcgtttaga aattcaaaat
     2581 gaaacagtct cagctcatgt aaactctcca gaagttggta aagttaatga ggaattggat
     2641 actgttagcc ttaaaggtga tagtttaaat attagtttta atccaactta cctaattgaa
     2701 tctttaaaag cagtaaaaag cgaaacagtt acgattcgat ttatttctcc agtacgtcca
     2761 tttactttga cccctggtga agatactgaa gatttcatcc aattaataac tcctgttcgt
     2821 actaactaaa taagaaaact acccaagtta ttcttgggta gttttcttat tctaagatat
     2881 gctataatga tgagtaatat gactctatat ataatagcta acccccatgc tggtaataaa
     2941 aatgcctcca ctattgttgg taaaattcag gagctttatc atactgaaga tatttctgtg
     3001 ttctatacag aacagaaaga tgatgaaaaa aaacaagtca ttaatatact aaggtctttt
     3061 aaagaaagtg atcatctaat gattatagga ggagatggta ccttatcaaa agtaatgact
     3121 tatctccccc aacatattcc gtgcgcttat tatcctgttg gttcaggaaa tgattttgcc
     3181 agggctttga aaattcctaa cctcaaagaa acccttaccg ctattcagac agaaagatta
     3241 aaggaaatta attgctttat ttatgataaa gggctgattt tgaatagttt agatttaggt
     3301 tttgcagcct atgtcgtttg gaaagcatct aactctaaaa taaaaaatat tttgaataga
     3361 tatcgtctag gaaaaataac ttacattgtt atagctatca aatcattatt acattcttct
     3421 aaagtacaag tattagtgga aggagagaca ggacagcgga taaaactaaa tgacctttat
     3481 tttttcgcat tagcaaataa cacttatttt ggaggtggca ttactatttg gcctaaagca
     3541 agtgctttaa ctgcagagtt agatatggtt tacgctaaag ggcatacttt tctaaaacga
     3601 ttaagtattt tattatcttt ggtttttaaa aggcatacta cctctaaaag tataaaacac
     3661 cagactttca aagctatgac cgtatatttt cctaaaaata gtttgattga aatagatggc
     3721 gaaatagttg agttagatca gattagttta aaatgtcaaa aaaggtattt atatatgtaa
     3781 ggagaaaaga tgtatcaagt tggatcactt attgaaatga aaaaacctca tgcttgtgta
     3841 attaaagaaa ctggtaaaaa ggctaatcaa tggaaggtac tgagagtagg agctgatatt
     3901 aaaattcagt gtactaactg tcagcatgtg attatgatga gtcgttatga ttttgagcga
     3961 aaattaaaaa aagtactgca accatgaaag tccttttaaa caaataatta tagctatatt
     4021 tgcaaccaat actttcctaa ataattgcta gtatgccgtt ggaaaattag ctattctaat
     4081 gttatcgaaa gaagaaaggt ggccattgac aatgacgaaa gttgcagaac agttgtttat
     4141 ttttcggtaa gtcatatcaa aatgggaaaa tggggatacc acaccagatt tggataattt
     4201 aatcaggtta gctgagattt ttgacgtgag cttagatgaa catgttttag ctaaaacaaa
     4261 tgaagttaaa gttgaacgta tttatgaaaa aaatcttgat ctgtaaaaat ataataaaat
     4321 ttattggttt atttttcgaa atattattcg atctctacta attatcttag ctatattaac
     4381 tttcttataa gctctaggtc ttccctttgt ttcggagtgg ataatttaaa tgaaggtgaa
     4441 agagtggcat aatgataagc aaatctgaaa ttttcttctt atcataccgc tttttcttct
     4501 attttttgat ataatggtct tgattgaaat ttgaatggag actcactaaa tggctttaac
     4561 agcaggtatt gtaggcttac caaatgttgg taaatcaact ttatttaacg caattacaaa
     4621 agcaggagca gaagctgcta actatccttt tgcgactatt gatcccaatg ttggtatggt
     4681 agaggtacca gatgagcgtc tgcaaaaatt gacagaattg attacgccta aaaaaacagt
     4741 tccgacaacc tttgaattta ctgatattgc aggtattgtt aaaggtgctt cgaaggggga
     4801 aggtctagga aataaatttt tagctaatat tcgtgaagtg gatgctattg ttcatgtggt
     4861 acgtgctttt gatgatgaaa atgttatgcg tgaacaaggt cgtgaggatg ccttcgttga
     4921 tcctatagca gatattgata ctattaatct tgaattaatc ttagctgatt tagagtcaat
     4981 caataaacgt tatgcgcgtg ttgaaaaaat ggcacgaact caaaaagata aagaatcagt
     5041 agcagagttc aatgttttac aaaagattaa acctgttttg gaagatggga aatcagctcg
     5101 taccattgag tttacagaag aggaagcaaa agttgttaaa ggtctctttt tattaacaac
     5161 taaacctgtt ttatatgttg ctaatgttga tgaggacaaa gtagctgatc ctgatgatat
     5221 cgactatgtt aatcaaattc gtgcatttgc agaaacagag agtgcagaag tagttgtgat
     5281 ttctgctcga gctgaagaag aaatatctga acttgatgat gaagataagt tagaattttt
     5341 agaagctatt ggattaacag aatctggtgt tgataaatta acacgagctg cttatcatct
     5401 tttaggcctt ggaacttact ttactgctgg tgaaaaagag gttcgcgctt ggacatttaa
     5461 gcgtggtata aaagctccac aagctgcggg tattatccat tcagattttg aaagaggttt
     5521 tattcgtgca gtaacgatgt catatgatga ccttattcaa tatggatctg aaaaagcagt
     5581 aaaagaagct ggccggttac gtgaagaagg aaaagagtat attgttcaag atggcgatat
     5641 catggaattc cgtttcaatg tctagtaata aataatcaat ttgttaactt ttaggttaag
     5701 gatattgtta ttatccttaa ccatttttgc gttttaatgg agaagataat ggtaaaaatg
     5761 attgttggat taggaaatcc aggaagtaaa tataatgata caaaacataa tattggattt
     5821 atggctgttg acagaattgt aaaaaaactt gacgtcaact t
//
LOCUS       DATROD010000107         5703 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.109, whole genome shotgun sequence.
ACCESSION   DATROD010000107 DATROD010000000
VERSION     DATROD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5703
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.109"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            103..1248
                     /locus_tag="VBC74_001726"
     CDS             103..1248
                     /locus_tag="VBC74_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6942658.1"
                     /translation="MGQEPIIEYQNINKVYGENVAVEDINLKIYPGDFVCFIGTSGSG
                     KTTLMRMVNHMLKPTNGTLLFKGKDISTINPIELRRRIGYVIQNIGLMPHMTIYENIV
                     LVPKLLKWSEEAKRAKARELIKLVELPEEYLDRYPSELSGGQQQRIGVIRALAADQDI
                     ILMDEPFGALDPITREGIQDLVKSLQEEMGKTIILVTHDMDEALKLATKIIVMDNGKM
                     VQEGTPNDLLHHPATSFVEQMIGEERLLHAQADITPVKQIMLNNPVSITTEKTLTEAI
                     TLMRQKRVDSLLVTDNGKLIGFIDLESLSSKYKKDRLVSDILKHTDFYVMEDDLLRNT
                     AERILKRGLKYAPVVDHENNLKGIVTRASLVDMLYDIIWGDTETEDQ"
     gene            1248..1883
                     /locus_tag="VBC74_001727"
     CDS             1248..1883
                     /locus_tag="VBC74_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6942659.1"
                     /translation="MVNFLSQYGMQILVKTWEQVYISFFAIALGIAIAVPLGVVLTRF
                     PKVAKIIIAIASMLQTIPSLALLALMIPLFGIGKVPAIVALFIYSLLPILRNTYIGMN
                     NVNPTLKDCAKGMGMKPIQSIFQVELPLATPIIMAGIRLSTIYVIAWATLASYIGAGG
                     LGDLIFSGLNLFQPKLILGGTIPVIILSLIIDYLLGLLETALTPRTTRREA"
     gene            1886..2812
                     /locus_tag="VBC74_001728"
     CDS             1886..2812
                     /locus_tag="VBC74_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEO6942660.1"
                     /translation="MLKKSHFLQIFTLCLALLTISGCQLTDTKKPGHTTIKVAAQSST
                     ESSIMANIVTELIHHELGYNTTLISNLGSSTVTHQALLRGDADIAATRYTGTDITGTL
                     GLKAVKDPKEASKIVKTEFQKRYNQTWYPTYGFSDTYAFMVTKEFARQNKITKISDLK
                     KLSTTMKAGVDSSWMNREGDGYTDFAKTYGFEFSHIYPMQIGLVYDAVESNKMQSVLG
                     YSTDGRISSYDLEILRDDKKFFPPYEASMVVNNSIIKKDPKLKKLLHRLDGKINLKTM
                     QNLNYMVDDKLLEPSVVAKQFLEKNHYFRGDK"
     gene            2813..3463
                     /locus_tag="VBC74_001729"
     CDS             2813..3463
                     /locus_tag="VBC74_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6942661.1"
                     /translation="MKQMNTFQQFIYYFQHNGSYILEQFIHHFLISIYGVILAAIVGI
                     PLGIFIARRGKIKTFVMGIANVIQTIPSLAMISIIMLGLGLGIKTVVATVFLYSLLPI
                     ITNTYTGIRNVDSDLLDAAKGMGMTKRQRLFMVELPLSISVIMAGLRNALVVAIGITA
                     IGAFVGGGGLGDIIIRGTNATNGGAIILAGSLPTALMAIFSDLILGGIQRMLEPRK"
     gene            complement(3525..4706)
                     /locus_tag="VBC74_001730"
     CDS             complement(3525..4706)
                     /locus_tag="VBC74_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6942662.1"
                     /translation="MNINGIKLLSSRAVSKLGDVLYDYGNSTWIASIGGLGQKILGIY
                     QIVELLVSIVLNPFGGALADRFQRRKILLITDAICAIMCFLLSFIGDDKVMVYGLIVA
                     NAILAVSNAFSSPAYKSYIPEIVDKADIITYNANLETIVQIISVSSPVLGFLIFNNFG
                     IRITLIVDAITFLISFLFLYAIKVERVQLSKQEKVAIKNILADIADGFTYIKKEKEIM
                     FFLIIAALLNTFLAMFNYLLPFTNSLLKTSGAYATILSISAIGSIIGALIARKIKSSI
                     NSMLSMLVFSSLGVIVMGFPSLFELPIWISYSGSFLFNSLLTMFNIHFFSQVQIRVDE
                     AYMGRVMSTIFTIAIMFMPIGTLFMTIFSFALSNVSFIVIGCAIAILGGLGFSYSKKQ
                     F"
     gene            complement(4779..5632)
                     /locus_tag="VBC74_001731"
                     /pseudo
     CDS             complement(4779..5632)
                     /locus_tag="VBC74_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285452.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MutR family transcriptional regulator"
BASE COUNT         1931 a         1020 c          922 g         1830 t
ORIGIN      
        1 ttaacgattc tttttaaaat cattaaattt taaaacaaat ttcagacata ttgccaaagt
       61 tttgatatta ttactataat atagtttgta gaggagaata atatgggcca agaacctatc
      121 atcgaatatc aaaatatcaa taaagtgtat ggggaaaatg ttgcggttga agatattaac
      181 cttaaaattt accctggtga tttcgtttgt ttcatcggta cgagtggatc aggtaaaaca
      241 acattaatgc gtatggttaa ccatatgtta aaaccaacaa atggtactct attatttaag
      301 ggaaaagata tctctactat taaccccatt gaattaagac gcagaattgg atatgttatc
      361 caaaacattg gtttaatgcc tcatatgacc atttacgaaa atatagttct tgtaccaaaa
      421 ttattgaaat ggtcagaaga agctaaaaga gctaaagcaa gggaacttat taaattagtt
      481 gaattacccg aagaatattt ggatcgctac cctagtgagt tgtctggcgg tcagcaacaa
      541 cgtatcggtg tcattcgcgc tcttgcagca gaccaagata ttattttaat ggatgagcct
      601 tttggagctc tggatcctat tactagagaa ggtattcaag acttagtcaa gtctcttcag
      661 gaagaaatgg ggaaaactat catcttagtt actcatgata tggatgaagc cctcaagtta
      721 gcaacaaaaa ttattgttat ggacaatggt aaaatggtcc aagaagggac acccaatgat
      781 ctcttacatc atcctgctac tagtttcgtt gaacaaatga ttggagaaga gcgtctcctt
      841 catgcgcagg ctgatattac tcctgttaaa cagataatgt taaataatcc tgtttcaata
      901 actactgaaa aaacactaac tgaagctatt acactaatgc gccaaaaacg cgttgactca
      961 cttctagtaa ccgataacgg taaattaatt ggttttattg acttagaatc tctaagcagt
     1021 aaatataaga aagatcgact tgtttctgat atcttaaaac atactgattt ttatgttatg
     1081 gaagacgact tacttagaaa tactgctgag cgtattttaa aacgtggttt aaaatacgct
     1141 ccagttgttg accatgagaa taacttaaag ggcattgtta ctcgtgcatc cctagttgat
     1201 atgttatacg atattatttg gggcgatact gaaacggagg atcaataatg gtcaatttct
     1261 tatcacagta tggcatgcaa atattagtaa aaacatggga acaagtctat atctcatttt
     1321 ttgctatagc acttgggatt gcaattgctg ttccattggg tgtggtctta acacgttttc
     1381 ccaaagtagc caaaattatc attgctatag ctagcatgct acaaactata ccaagtttag
     1441 ccttattagc tttaatgatt ccattgtttg gaattggaaa agtcccagct atcgttgctt
     1501 tatttattta ttccttgctt cctatactaa ggaatactta tataggtatg aataatgtca
     1561 atccaacact taaagattgt gctaaaggga tgggaatgaa acctattcaa tctatatttc
     1621 aagttgagtt acctttagct actccaatta ttatggctgg tattcgcttg tctactattt
     1681 atgttattgc ttgggctaca ttagcttctt atataggtgc aggtggactt ggtgatctca
     1741 tttttagcgg gttaaatctt tttcagccca aattaattct agggggcaca attcctgtta
     1801 ttatcctgtc acttattata gattatcttt taggattact ggaaacagct ctaacgccca
     1861 gaacaacaag aagggaggct tagatatgct taaaaaatcg cactttttac agatatttac
     1921 actttgctta gccctcttaa cgatttctgg ttgtcaatta accgatacta aaaaacctgg
     1981 tcataccaca attaaggttg ctgcccaaag ttctacagag tctagtatca tggcaaatat
     2041 tgtcaccgaa ttaattcatc acgaattagg atacaacaca actttaataa gcaatcttgg
     2101 ttcctctacg gttactcacc aagctttgct ccgtggtgat gctgacattg ctgccacacg
     2161 ttatacagga acagacatca caggaactct tggcttaaaa gctgttaaag accctaaaga
     2221 agcttctaag attgtaaaaa ctgaattcca aaaacgctac aatcaaactt ggtatcctac
     2281 ttatggtttt tctgatactt atgcattcat ggttactaaa gagtttgcca gacagaataa
     2341 aatcaccaag atctctgatc tcaaaaagtt atcaacaact atgaaggcag gggttgatag
     2401 ttcatggatg aatcgcgagg gagatggata cactgatttc gctaaaacat acggttttga
     2461 attttcacat atttacccta tgcaaattgg cttagtctat gatgcggttg aaagtaacaa
     2521 aatgcaatct gtattaggct actccactga cggtcgtatt tcgagctatg atttagaaat
     2581 tttaagggat gataaaaaat tctttcctcc ttatgaagcc tctatggttg tcaacaattc
     2641 tatcatcaaa aaagatccta aactaaaaaa attactccat cgactcgatg gtaaaatcaa
     2701 tttaaaaacg atgcaaaacc ttaattatat ggtagatgat aaacttttag aaccttcagt
     2761 tgttgccaaa caatttttag aaaaaaacca ttattttaga ggagataaat aaatgaaaca
     2821 aatgaatact ttccaacaat ttatctacta cttccaacat aatggtagtt atatcttaga
     2881 acaatttatc caccactttt taatttcaat ttacggcgtt attttagctg caattgttgg
     2941 tatccctcta ggtatcttca tcgctagacg tggtaaaatt aaaacctttg ttatgggaat
     3001 cgcaaatgtc atccaaacca ttccttcttt agctatgatc tctatcatta tgttaggtct
     3061 agggctaggt attaaaacag tagttgcaac tgtctttctt tattctcttc tgccaatcat
     3121 cacaaatact tacactggta ttagaaatgt tgactccgac ttactagatg ctgctaaagg
     3181 aatgggcatg actaagcgtc aacgtttatt tatggttgag ttaccactat ctatttctgt
     3241 tatcatggct ggacttagaa atgcccttgt tgtagcaatt ggtattacag ccattggtgc
     3301 tttcgtcggt ggaggcggac ttggagatat catcataaga ggaactaatg caacaaatgg
     3361 tggtgctatt atcttagcag gatctcttcc aacagccctt atggcaattt tctctgattt
     3421 aatcctaggc ggtatccaac gcatgcttga accaagaaaa taactattaa aaacttgtca
     3481 gtagagacaa gtttttttga tgtttcagaa taatttctta agttctaaaa ttgtttttta
     3541 ctatagctaa atcctaatcc tcccaatata gcaatggcac atcctataac aataaagcta
     3601 acatttgata aggcaaatga aaagatagtc ataaatagtg ttcctatagg cataaacata
     3661 atagcaatag taaatatagt cgacatcaca cgccccatat acgcttcatc gaccctgatt
     3721 tgtacctgac tgaaaaaatg aatattaaac attgtcagaa gactattaaa aagaaaacta
     3781 ccactgtaag atatccatat cggtaattca aatagagatg ggaatcccat aactatgact
     3841 cccaaactac tgaatactag catagatagc atagaattaa tactagattt aatttttcta
     3901 gcaataagcg ctccaataat tgatccgata gctgatatac taagtattgt tgcataagca
     3961 cctgaagttt tcaataggga gttagtaaaa ggtaaaagat aattgaacat tgctaggaaa
     4021 gtgtttaata aagcagcaat aattagaaaa aacataattt ctttttcttt tttgatgtaa
     4081 gtaaatccat ctgcaatatc agctaaaata ttttttatcg ctaccttttc ttgttttgaa
     4141 agctgtaccc tttccacttt aatagcataa agaaacagga aagagatcaa aaaagtaatc
     4201 gcatcaacaa ttaaagtaat cctaatacca aaattattaa aaataaggaa acctaaaaca
     4261 ggggaactta cactaataat ttgcacaatg gtttccagat tagcattata ggtaatgata
     4321 tcagctttat ctactatttc aggaatataa gatttataag ccggagaaga aaaagcattg
     4381 gaaaccgcta agatagcatt agcaactatt agaccataca ccattacttt atcatctcca
     4441 ataaaagaaa gtaagaaaca catgatagca cagatagcat ctgttattag taaaattttt
     4501 cttcgttgaa agcgatcagc taaggcgcct ccaaaagggt tgaggacaat agaaaccaac
     4561 aactctacta tctgataaat acctaaaatc ttttgtccta gaccacctat cgaagcaatc
     4621 caagtactat ttccataatc ataaagcaca tcaccaagtt tactgactgc tcgactagat
     4681 agtaatttta taccattaat attcataatt ccctccttag ttcgacatta tagcaaacca
     4741 aaaggagaaa tattaaaaaa tcccataaat gggatttttt ataatcttgt taccctatta
     4801 aagtgttcct gaaaattttc aacatgatgt ttagaaccaa ttgcttggaa gcaaaaaata
     4861 gcttgcttca tgctttgtat tcctcgacta tcccctttag cataggcaag aaatcctttt
     4921 tcatagagta aaataatacg gtgataagca ttccgctcat tatttaaaag tgcttcaact
     4981 ttctctagaa aaaacgttgc ttcttctaga gcgcgccgtt ctattagtgt ctctaccaca
     5041 tttaggaggg ttgcttcgac aacattgcgg tttgtataaa tatctcgata atattccttt
     5101 tgattgataa cctctttaac aagttggcaa atatgccttg tgttgtaata ccggtaaaga
     5161 tttccaatca aaatatagtc actaatttcc caactatcct tttgaaagag gtaatccgac
     5221 aaaaagcaaa ggtcttcttc cgacattttt cttgaggagt catattcaca tatcattcct
     5281 ctaaaaagaa ctatgtttaa acgacaacgt atatctgaac tatccttttt tagtttacct
     5341 tcttccacag aaatcatctt ttctaaccca gctacgtctc tttgataatg atattgagcc
     5401 atctgagaca ttactgatca gatttttgat atttatttac tctatccata aattcgctaa
     5461 ttgataaatc aattgcctct aaagccccta aaaattttgt taatgataaa tctgattcac
     5521 ccctctcaaa tcgtgataac tgagaaacag aaagttcatt tgaagcaacc tgtttaagag
     5581 aaaactgtcg attaagtcga aattccctaa aaatagggcc atatataaac attagtattc
     5641 ctttctaacc gtaatctcat tggtaaactt atgaggtaat ttcaataatt tatatttacc
     5701 aac
//
LOCUS       DATROD010000108         5690 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.110, whole genome shotgun sequence.
ACCESSION   DATROD010000108 DATROD010000000
VERSION     DATROD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.110"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            44..277
                     /locus_tag="VBC74_001732"
     CDS             44..277
                     /locus_tag="VBC74_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6942663.1"
                     /translation="MSESKLRKLFKQEKHITIQQYFLNLKIEAAKQLLDENKKVEEVS
                     NLLGFSTSSNFSRTFKKIVGISPLEYKQKPKTI"
     gene            complement(472..996)
                     /locus_tag="VBC74_001733"
     CDS             complement(472..996)
                     /locus_tag="VBC74_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO6942664.1"
                     /translation="MVKDRQIQKTKVAIYNAFISLLQENDYSKITVQDVIGLANVGRS
                     TFYSHYESKEVLLKELCEDLFHHLFKQGRDVTFEEYLVHILKHFEQNQDSIATLLLSD
                     DPYFLLRFRSELEHDVYPRLREEYITKVDIPEDFLKQFLLSSFIETLKWWLHQRQKMT
                     VEDLLKYYLTMVER"
     gene            1128..1991
                     /locus_tag="VBC74_001734"
     CDS             1128..1991
                     /locus_tag="VBC74_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEO6942665.1"
                     /translation="MTSNKKVAIAFILNISFSVLEFIFGSLFFSGAILADAVHDFGDA
                     IAIGISATLEKKSKKDEDTIFSLGYKRFSLLGALITSLILISGSILVMIENIPKLWHP
                     TPVNYHGMFILAVIAIIINGLASFILHSGQSKHEEILSLHFLEDILGWLAIIVISLIL
                     NWKPLYILDPLLSVAISTFILSKALPKLLSTLKLFLDGVPDSIDYAALHDELKGLSQV
                     RSINQLNIWSMDGIDNRAIIHCCLNQLISEKDCKRAIRTICQHYKINDVTVEIDYSLR
                     EHQNHCKPLKN"
     gene            2076..2930
                     /locus_tag="VBC74_001735"
     CDS             2076..2930
                     /locus_tag="VBC74_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEO6942666.1"
                     /translation="MTDTMILNDNHKIPTLGLGTWMIDNDRVAQVVKDAIELGYRHID
                     TAQAYNNEKGVGVGIKESGITRESLFVTTKIAAEHKDYESATKSIDRSLEALGLDYID
                     LMIIHSPQPWKEWRETDKHFDQGNLEAWRALEDAQKSGKVKSIGVSNFLEKDLENILK
                     NGYVKPAINQILAHIGNTPFDLIDYCQSKGIQVEAYSPIAHGQALKSDGIQKMAEKYG
                     VSVAQLCIQYLLQLNLIVLPKASSKEHLQSNLDFDFVISDEDMSILKSLMFDDYGEFS
                     NFPVFSGK"
     gene            3040..4067
                     /locus_tag="VBC74_001736"
                     /pseudo
     CDS             3040..4067
                     /locus_tag="VBC74_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861552.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            4083..4472
                     /locus_tag="VBC74_001737"
     CDS             4083..4472
                     /locus_tag="VBC74_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEO6942667.1"
                     /translation="MHIKEVSKKTGITSDTIRYYERINLIPPIHRSESGIREFTQNDI
                     DILEFVRYFRGAGVSVESLIDYIHLVEKGDSTIPARLEILKDKRNNLEDRLQGLQEAL
                     NRLNHKIDNYQKVLVPREQELFDPTTK"
     gene            4482..4877
                     /locus_tag="VBC74_001738"
     CDS             4482..4877
                     /locus_tag="VBC74_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001158469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEO6942668.1"
                     /translation="MQETTKSIFPKGEKNPYGEFFIRQSYLAALAKSPDGNVSVGNVT
                     FEAGCRNNWHVHLDGYQILLVTEGSGWYQEENKKAVSLEPGNVIVTDKGVKHWHGAKK
                     DSEFAHIAITAGKSEFYEAVSLEEYSRLG"
     gene            4905..5222
                     /locus_tag="VBC74_001739"
                     /pseudo
     CDS             4905..5222
                     /locus_tag="VBC74_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660666.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            5309..5593
                     /locus_tag="VBC74_001740"
     CDS             5309..5593
                     /locus_tag="VBC74_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HEO6942669.1"
                     /translation="MLTIQEMKDTTFELQENFRRLNYPIKQVAKDLQLNISEVESLLS
                     LDVTYPGDVCMLRDYLEDMLKKEGKEVYPFSRLASHSANRWYPYETPWRY"
BASE COUNT         1955 a          908 c         1014 g         1813 t
ORIGIN      
        1 aaatcgcttt attaaaacag aagaaatagc taaagaatgt cacatgagtg aatcgaagtt
       61 acgaaaacta tttaagcaag aaaaacacat cactattcaa caatattttc taaacttaaa
      121 aatcgaagct gctaagcaat tactagatga aaataagaaa gtagaagaag tttccaattt
      181 acttggattt tccacttctt ctaatttttc aaggacattt aaaaaaatag tgggaattag
      241 cccactagaa tataagcaaa agcctaagac aatatagtaa tcgaaaaata ttcattttgc
      301 ttatggtgac atcaacaatc ccaaagaaac tctttcatca aggggacttt tttatctccc
      361 gaattatact catttaatct aataatttca actcgctata ttagacaatc aatccattca
      421 tcacctctga taacgctaat gaaataagag aatctcccaa ctctctataa tttacctctc
      481 taccatagtt aaataatatt ttagtaaatc ttcaacagtc attttctgtc tttgatgaag
      541 ccaccacttt aaggtctcga taaaactgga taaaagaaat tgttttaaga aatcttcagg
      601 aatgtccacc tttgtaatat actcctcacg caatctaggg taaacatcgt gttctaattc
      661 acttctaaag cgtaataaaa aatagggatc gtcagataat aataacgtag cgatactatc
      721 ttgattttgt tcaaaatgtt ttaagatatg aactaaatac tcttcaaacg taacatctct
      781 accctgctta aataagtggt gaaaaaggtc ttcacagagc tccttgagta acacttcttt
      841 gctttcatag tgagagtaaa aagttgatct tcctacattt gcaagtccga ttacatcttg
      901 aaccgttatt ttagaataat cattttcctg tagcaagctt ataaaagcat tataaatcgc
      961 tacttttgtt ttctgaattt gtctatcttt taccatatag gacactttct aagttttgtt
     1021 cagtattgaa caagtgttct tttttgtttc tagcaattct cttttatctc ctagataata
     1081 gaagaaagaa tcacactaca ataccagtat aacaaaagga gaaaaatatg acatcaaaca
     1141 aaaaagtggc tatcgctttt atattaaata tcagcttttc cgtccttgaa ttcatctttg
     1201 gaagtctatt cttctcagga gctatcttag ccgatgctgt tcatgatttt ggtgatgcaa
     1261 tagctattgg catctcagct actttagaaa aaaaatctaa gaaagatgaa gatacgatat
     1321 tttcattagg ttataaacgt tttagccttt taggagctct tatcacaagt ctaattctta
     1381 taagtggatc tatcttagtc atgatagaaa atattccaaa attgtggcat cctactcctg
     1441 ttaattatca cggcatgttt attctagctg tcatcgctat tatcattaat ggtctagcta
     1501 gttttattct ccattctggt caaagtaaac atgaggaaat attaagcctt cattttttgg
     1561 aagatattct agggtggtta gctattattg ttatatctct tatcttaaat tggaaaccgt
     1621 tgtatatcct tgatccatta ttatcagttg ccatatctac tttcatctta tctaaagcac
     1681 ttcctaaatt actatcaacc ctgaaacttt ttttagatgg tgttccagat tccatagatt
     1741 acgctgcatt acatgatgaa cttaagggac tttcgcaagt aagatctatc aatcaactca
     1801 atatctggtc aatggatggt attgataatc gagcaatcat tcactgttgc ttaaatcaat
     1861 tgatatctga aaaagattgt aaaagagcta ttcgaacgat ttgccaacat tataagataa
     1921 acgatgttac tgttgaaatc gattattctt taagagaaca tcaaaatcat tgcaaaccat
     1981 taaaaaacta acctgagtgt tagttttttc cttgacttgg agttaactct aagtgttaag
     2041 attaggtcaa tactaatgaa agaaggaaaa taaagatgac agatacaatg atattaaatg
     2101 acaatcataa aataccaact ttaggtttag gtacgtggat gatagataat gatagggtag
     2161 ctcaagttgt taaagatgct atcgaattag gctaccgcca tattgatact gcacaagcat
     2221 acaacaatga aaaaggggtc ggtgtaggga ttaaagaatc tggtattaca cgtgaatctt
     2281 tatttgttac gactaaaatt gccgctgaac ataaagatta tgaaagtgct acaaaatcaa
     2341 ttgatcgatc actagaagct ttagggcttg attatattga tttaatgatt attcatagtc
     2401 cacagccttg gaaggaatgg cgtgaaacag ataaacattt tgaccaagga aatttagaag
     2461 cttggagagc tttagaggat gctcaaaaat ctggtaaggt aaaatccatc ggtgtctcaa
     2521 actttttaga aaaagactta gaaaatatct taaaaaatgg ttatgttaaa cctgctatca
     2581 atcaaatctt agctcacatc ggaaatacac ctttcgactt gattgattac tgtcaaagta
     2641 aaggtattca agttgaagct tattcaccaa ttgcacatgg acaagcattg aaatctgatg
     2701 gtattcaaaa aatggctgaa aaatatgggg ttagtgttgc tcaattatgt attcaatatt
     2761 tactacaatt aaacctgatc gttttaccaa aggcttctag taaagaacat ttacagtcta
     2821 atctagactt tgactttgta attagtgacg aagatatgtc gatattaaaa tcacttatgt
     2881 ttgacgatta tggggaattt tctaacttcc ctgtttttag tggaaaatga aaccatatgc
     2941 ggatttttac agatgtttaa ttttcaaaat ttttaagttt ttcttgactt gaagttaact
     3001 ctaagagtta taatgaagtt atagaaaaga ggtaaggtta tgaaagtagc tacatttatt
     3061 gaacctggca aaatggttat aactgataca ccaaaaccag tcattgaaca agagacagat
     3121 gctgttatca aaattgttag agcctgtgtt tgtggttcag acttgtggtg gtatcgtggt
     3181 atttcaaaac gtgaaagtgg ttcttttgca ggtcatgagg ctattggtat cgttgaggaa
     3241 gttggtacta aagtaactga cgtgtcaaaa ggtgattttg ttattgttcc ctttacacat
     3301 ggctgtggcc aatgtccgtc ttgtaaggct ggatttgatg gaaattgtac aaatcatcaa
     3361 gctgaaaaaa atgtagatac caaggtcaat atctacgtta tactaatgca aactgggcat
     3421 tggttaaaat tcctggtcaa ccttctgact atgataatga gacacttaac tctcttctca
     3481 ccttatcaga tgtaatggca actggttatc atgctgcaga agttaaagaa ggtgatacgg
     3541 tagttgtcat gggagatggt gctgttggac tctgtggtgt tattgctgct aaaatgttag
     3601 gtgctaaccg tatcattgca atgagtcgtc acaaagatag gcaggaacta gcattgactt
     3661 ttggtgcaac ggatattgtc gaagaacggg gtgacgaagc cgttaaacgt gttttagatt
     3721 taaccaatca agcaggtgcc gatgctattt tagaatgcgt tggtacagag cagtctgttg
     3781 atacagctac ccaaattgct aggcctggtg ctgtcatagg acgtgttggt atcccacaaa
     3841 atccagacat gaataccaat aatttattct ggaaaaatat cggccttaga ggtggcattg
     3901 cttctgtaac aacatttgat aagtctgtcc ttttagatgc tgttctaact cataaaatta
     3961 acccaggctt agtttttaca aaatcctttg tattagatga tattcaaaag gcttatgaag
     4021 caatggataa acgtgacgct atcaaatcct tagtgattgt tgactgaata tcgatgttaa
     4081 aaatgcatat caaagaagtt agtaaaaaga ctggcattac gtcagatacc attaggtatt
     4141 atgagagaat taatcttatt ccccctattc atcggagtga atcaggaatt cgtgagttca
     4201 cacaaaacga tattgatatt ttagaatttg tccgctattt tagaggcgca ggtgtttccg
     4261 ttgaaagtct cattgattat attcatttgg ttgaaaaagg cgattccaca atcccagcta
     4321 ggctagaaat tttaaaagat aaacgcaaca accttgaaga taggcttcaa ggcctccaag
     4381 aagcacttaa tcgtctaaat cacaaaatag acaattatca aaaagttctt gttcctagag
     4441 aacaagaact ttttgatcca acaacgaaat gaggaaatct tatgcaagaa acaactaagt
     4501 ctatctttcc taagggtgaa aaaaatccat atggtgaatt ctttattagg caatcctatc
     4561 tcgcagcatt agcaaaatct ccagatggga atgtttcagt tggaaatgta acctttgaag
     4621 ctggctgtcg taataattgg catgttcatt tggacggtta tcaaatcctc ctcgttacag
     4681 aaggttcagg ttggtaccaa gaagagaata aaaaggcagt ttcattagaa ccaggtaacg
     4741 tgattgtaac tgataaaggc gttaaacatt ggcatggtgc taaaaaagat tctgaatttg
     4801 cacatattgc gattacagct ggtaagagtg agttttatga agctgttagt cttgaagaat
     4861 attcaagact aggttaaatt caagtaagga ggaatcaatc cattatgaaa gaaaaacaaa
     4921 cagccggacg tcgtcaatta gaagaatttg cacctgaatt tgccagatat aatgatgata
     4981 ttttatttgg tgaggtttgg gcaaaagagg atcatttaac agataaaaca cgttctatta
     5041 ttactatatc tgccttaatt agctgaggaa atcttgaaca gttagagcat catcttcaat
     5101 ttgccaaaca aaacggagtt actaaagaag aaattgctga cattatcact cacctcgctt
     5161 tttatgttgg atggccaaaa gcttggtcag cctttaataa agctaaggaa atttggatat
     5221 aaaataacag acatagcaag ggagatactt tcccaaaagc caaaaagaaa gatcaaccaa
     5281 ttattaaacc aactattagg agaatgatat gcttactatt caagaaatga aagatacaac
     5341 ctttgaatta caagaaaatt ttagaaggtt aaattatcct attaaacaag ttgctaaaga
     5401 cttgcaactt aatatcagtg aagttgaatc tcttttaagt cttgatgtta cttatcctgg
     5461 agatgtttgt atgctgagag attatctaga agatatgttg aaaaaagagg gtaaagaagt
     5521 ttatccattt tctagattag catctcattc agcaaatcgt tggtaccctt atgagacacc
     5581 atggcgttat taaagactaa agattaatcc atcatcttta gagaaataaa caaaaatagt
     5641 caatagattc tttttagaaa aaagctataa aataagtgcc tcttagagaa
//
LOCUS       DATROD010000109         5574 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.111, whole genome shotgun sequence.
ACCESSION   DATROD010000109 DATROD010000000
VERSION     DATROD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.111"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            246..635
                     /locus_tag="VBC74_001741"
     CDS             246..635
                     /locus_tag="VBC74_001741"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEO6942670.1"
                     /translation="MGNIELKKLAVKASENAYVPYSKFPVGAALRTAEGKIFTGCNVE
                     NISYGLANCAERTAIFKAVSEGYKDFSEIAIYGNTEEPISPCGACRQVMVEFFNKNAK
                     VTLIAKNGKTVETTVGELLPYSFVDLK"
     gene            700..1749
                     /locus_tag="VBC74_001742"
     CDS             700..1749
                     /locus_tag="VBC74_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019315854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEO6942671.1"
                     /translation="MNKKIAGIGLASIAVLSLAACGHRGASKSGGKSDSLKVAMVTDT
                     GGVDDKSFNQSGWEGMQAWGKKNGLKKGAGFDYFQSASESDYATNLDTAVSSGYKLIF
                     GIGFSLHDAIDKAADNNKDVNYVIVDDVIKGKDNVASVVFADNESAYLAGIAAAKTTK
                     TKTVGFVGGMESEVITRFEKGFEAGVKSVDKSIKIKVDYAGSFGDAAKGKTIAAAQYA
                     SGADIVYQVAGGTGAGVFSEAKSRNESLKEADKVWVLGVDRDQAAEGKYTSKDGKASN
                     FVLASSIKEVGKSVELIATKTSKGKFPGGNVTTYGLKDGGVDIATTNLSDDAVKAIKE
                     AKAKIISGDIKVPSK"
     gene            1894..3429
                     /locus_tag="VBC74_001743"
     CDS             1894..3429
                     /locus_tag="VBC74_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6942672.1"
                     /translation="MTQNVIEMKEITKKFGDFVANDHINLTVEKGEIHALLGENGAGK
                     STLMNMLAGLLEPTDGQIFINGQPVTIDSPSKSSQLGIGMVHQHFMLVEAFTVAENIV
                     LGNETTQNGVLDIKTAAKEIKELSEKYGLSVNPNAKISDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPSEIKELMTIMKSLVKEGKSIILITHKLDEIRAVADKVTVIRRGK
                     SIETVPVAGASSQQLAEMMVGRSVSFRTEKKEANPTDIILSVKDLVVEENRGGVLAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVTSGQIVIKGKDVTKFSSRQITEL
                     SVGHVPEDRHRDGLVLDMTMAENLALQTYYKEPLSHKGILNFAKIKEYARQLMTEFDV
                     RGAGEHVLARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DKGKAVLVVSFELDEILNLSDRIAVIHDGKIQGIVKPDQTNKQELGILMAGGKIEKEE
                     RDV"
     gene            3422..4483
                     /locus_tag="VBC74_001744"
     CDS             3422..4483
                     /locus_tag="VBC74_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6942673.1"
                     /translation="MSKKAQKIAVPLISVVLGIILGAIIMLIFGYDPLWGYEGLFQTA
                     FGSIKNIGEIFRAMGPLILIALGFSVASRAGFFNIGLPGQALSGWIAAGWFALSHPDM
                     PRPAMILCTIIIGIVAGGITGAIPGILRAYLGTSEVIVTIMMNYIVLYSGNAIVQRVF
                     PKSIMRTSDSSVYVSANASYQTDWLSSLTNNSRINIGIFIAIIAVVLVWFLLNKTTLG
                     FEIRSVGLNPNASEYAGMSAKRTIILSMIISGAFAGLGGVVEGLGTFENVFVQPSSLA
                     IGFDGMAVSLLAANSPIGILFAAFLFGVLSVGAPGMNIAGIPPELIKVVTASIIFFVG
                     VHYIIEYVIKPKKQMKGGK"
     gene            4485..5441
                     /locus_tag="VBC74_001745"
     CDS             4485..5441
                     /locus_tag="VBC74_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6942674.1"
                     /translation="MSLTTIFALLFSSMLIYATPLIFTSIGGTFSERGGIVNVGLEGI
                     MVIGAFSGVVFNLEFASVFGDATPWISVLVGGLVGLIFSVIHAVATVNFRADHIISGT
                     VLNLMAPSLAVFLIKVLYNKGQTDNIQESFGKFNFPILSDIPFVGDIFFKGTSLVGYI
                     AILFSFLAWFILYKTRFGLRLRSVGEHPQAADTLGINVYLMRYSGVLISGFLGGIGGA
                     VYAQSISVNFAATTILGPGFISLAAMIFGKWNPIGAMLASLFFGLSQSLAVIGSHLPL
                     LSNIPTVYLQIAPYVLTIIVLAAFFGQAVAPKADGINYIKTK"
BASE COUNT         1753 a          824 c         1175 g         1822 t
ORIGIN      
        1 aatgttttct ttaaattccg tataagataa aaagaattct gctttttatt ctgaaaatta
       61 taaaaaacta cttacattat ctaaaaaaaa tggtaaaata gattcagcta aaataccaag
      121 actaatgaaa ctacaagact tggttactca ataataaata cttctgtgtt tgataagaag
      181 ataataaacg tgttctaaaa attttgccag taaacattat gaagcgaaag gtgcagctac
      241 taaaaatggg aaatatagag ttgaaaaaat tggctgtaaa agcgagtgaa aatgcatacg
      301 ttccttactc gaaatttcca gtaggcgcag cattgcgtac agcagaagga aaaatattta
      361 caggatgtaa cgttgaaaat atcagttatg gattagcaaa ctgtgctgag cgtactgcaa
      421 tttttaaagc tgtttcagaa ggttataaag atttttcgga aattgctatt tatggaaata
      481 ctgaagaacc aatttctcct tgcggtgcat gccgtcaagt gatggtggaa ttttttaata
      541 agaatgctaa agttacattg attgctaaaa atggtaagac ggtcgagacg acggtcggtg
      601 agttgttacc atactcgttt gttgatttaa aataatttaa atcagttgct cttttaagca
      661 tttagcttaa atattaagtt ttttaggagg gttcatttca tgaacaaaaa aattgccggg
      721 atcggcttgg cttcgattgc agtacttagt ttagctgcat gtggacatcg tggtgcttct
      781 aaatctggtg gtaaatcaga tagcttgaag gttgcaatgg taacagatac cggtggtgtt
      841 gatgataaat catttaacca atctggttgg gaaggtatgc aagcttgggg caagaagaat
      901 ggccttaaaa aaggagctgg ttttgactat ttccaatcgg caagtgaatc tgattatgca
      961 actaacttag atacagctgt gtctagtggt tataaattga ttttcggtat tggattttct
     1021 cttcatgatg ctattgataa agcagcagac aataacaaag atgttaatta cgtcatcgtt
     1081 gatgatgtta ttaaagggaa agataatgtt gcaagtgttg tctttgcgga taatgaatca
     1141 gcttacttag caggtattgc agccgctaaa actaccaaaa caaaaacagt tggctttgta
     1201 ggtggtatgg aatctgaggt tattacccgt tttgaaaaag gttttgaagc aggtgtcaaa
     1261 tcagttgata aatcaattaa aattaaagtt gactatgctg gttcattcgg tgatgctgct
     1321 aagggtaaga caattgcagc cgcacaatat gcttctggcg cagatattgt ttatcaagta
     1381 gctggtggaa ctggtgcagg tgtattcagt gaagcaaaat cacgcaatga atctttaaaa
     1441 gaagctgaca aagtttgggt attaggtgta gaccgtgacc aagcagcaga aggtaagtat
     1501 acttctaaag atggtaaggc ttctaacttt gttttggctt catcaattaa agaagttgga
     1561 aaatcagttg aattgattgc tactaaaaca agtaaaggta aattcccagg tggtaatgta
     1621 acaacttatg gtttgaagga tggtggagtt gatatagcaa ctacaaacct ttcagatgac
     1681 gccgttaaag cgattaaaga ggctaaagct aaaattattt caggagatat caaagttcct
     1741 agcaaataat atctttaaga actttcaaaa aagcgattag aaatcgcttt tttgaagctg
     1801 agataaactt atatctcagt aaaatccatc agtcgttaac gattggtgga ttttactgaa
     1861 ctataaatga atttttctga aagggagaac cacatgacac agaatgtcat tgaaatgaaa
     1921 gaaatcacta agaaattcgg tgattttgtg gcaaatgatc atatcaattt gacggttgaa
     1981 aaaggtgaga tacatgctct gcttggcgaa aacggtgcag ggaaatcaac tttaatgaac
     2041 atgttagcag gtttactaga accaacagac ggtcaaattt ttattaatgg tcagcctgta
     2101 actattgatt caccttccaa atcgtcacaa ttggggattg gtatggtaca tcagcatttc
     2161 atgcttgttg aagcatttac tgtagctgaa aatattgttc taggaaatga aaccacccaa
     2221 aatggtgttt tagacattaa gacagcagct aaagaaataa aggaactatc agagaaatat
     2281 ggtttgtcag taaatccaaa tgctaaaata tctgatattt ctgtaggagc acagcagcgt
     2341 gttgaaattt tgaagacact ttatcgcggt gcagatattt taatttttga tgagccgacg
     2401 gcggtgctta caccatcaga aataaaagaa ttgatgacaa ttatgaagag ccttgttaaa
     2461 gaaggcaaat ccattatact aattacccat aaattggatg aaattcgggc agttgctgat
     2521 aaagttactg tgattcgccg tggtaaaagt attgaaacag tccctgttgc aggcgcaagt
     2581 tctcaacaac tggcggaaat gatggttgga cgatctgttt ccttcagaac tgaaaaaaag
     2641 gaagctaatc cgactgacat aatcctttct gtaaaggatt tagttgtaga agaaaataga
     2701 ggcggcgttc tagctgttaa aaatctgtcg ttagatgttc gagcaggtga aattgtaggt
     2761 atagctggta ttgatggtaa tggacaaagt gaattaattc aagctattac aggtttaaga
     2821 aaggtaactt ctggtcaaat tgtaattaaa ggtaaagatg tcacaaaatt ttcaagtcga
     2881 caaattacag aattaagtgt aggtcatgtt ccagaagatc gtcatagaga tggtttggtt
     2941 ctagatatga cgatggcaga aaatttagct ttacaaactt attataaaga gccattgagt
     3001 cataaaggta tccttaattt tgctaaaatt aaagagtatg ctcgtcaatt aatgacagaa
     3061 tttgatgtgc gtggtgctgg tgaacatgtt ttagcaagag gattttctgg tggtaaccaa
     3121 caaaaagcta ttattgctcg tgaagtggat cgcgatccag acctactcat tgttagccaa
     3181 ccgactcgtg gtctagatgt tggtgctatc gagtatattc ataaacgttt gattgaagag
     3241 cgcgataaag ggaaagcggt cttagtagtt agctttgagt tagatgaaat cttaaactta
     3301 tcagatcgta ttgcagttat tcatgatggt aaaattcaag gaattgttaa acccgaccaa
     3361 accaacaagc aagaattggg tattttgatg gctggtggaa aaattgaaaa ggaggagaga
     3421 gatgtctaag aaggcgcaaa aaattgcagt tcctctgatt tcagttgtac taggaatcat
     3481 tctaggagct attattatgc tgatatttgg ttatgatcca ctttggggtt atgagggatt
     3541 gttccaaaca gcattcggta gtataaaaaa tattggtgaa attttccgtg caatgggacc
     3601 attaatttta attgctctag gtttctcagt tgccagtcgt gcaggtttct ttaatattgg
     3661 acttccaggg caagccttat caggatggat tgccgcaggt tggttcgctc tttcacaccc
     3721 agatatgcca cgtccagcaa tgattctttg tactattatt attggtatcg ttgctggggg
     3781 aatcactgga gctattccag gtattctcag agcatacctt ggaacaagtg aagttattgt
     3841 aactattatg atgaactata ttgttcttta ttcaggaaat gctattgtac aacgtgtttt
     3901 cccaaaaagc attatgcgta caagtgactc gagtgtatat gtttctgcca atgcatcata
     3961 ccagacagat tggttatcta gtttaacgaa taattcacgt atcaatattg gtatcttcat
     4021 tgctattatt gcagttgtgt tagtttggtt cctattaaat aaaacaacgc ttggatttga
     4081 aattcgttca gttggtttaa atccaaatgc tagtgagtac gctggtatgt ctgcaaagcg
     4141 tacaattatt ctatcaatga ttatttctgg agcttttgca ggacttggtg gtgttgttga
     4201 aggactaggt acatttgaga atgtatttgt acagccatcc tcgttagcta ttggctttga
     4261 cggtatggca gtaagtctac tcgcagcgaa tagtccaata ggtattttat ttgcagcatt
     4321 tctatttggt gttctaagtg ttggtgcgcc tggtatgaac attgcaggta ttccacctga
     4381 gttaattaaa gttgtaacag cttcaatcat cttcttcgta ggtgtccatt acattattga
     4441 gtatgttatt aaacctaaaa aacaaatgaa aggtggtaag taaattgagt ttaacaacga
     4501 tttttgcatt gctattttca tcaatgctaa tttacgcaac acctcttatc tttacaagta
     4561 ttgggggaac cttctctgaa cgtggtggta tcgtcaacgt tggtttagaa ggaattatgg
     4621 taattggagc tttctcaggc gttgtattta atttagagtt tgcttctgtt tttggtgatg
     4681 cgactccatg gatttctgta ctagttggtg gtttagttgg tttaattttt tctgttatcc
     4741 atgcagttgc gacagttaat tttcgtgcag atcatattat cagcggaacc gttcttaatt
     4801 taatggcacc ttcattagct gttttcttga ttaaggttct ttataataaa ggtcaaactg
     4861 ataatattca agaatcattt ggaaaattta atttccctat actatcagat attccttttg
     4921 ttggtgatat cttctttaaa ggaacaagct tagtcggtta tattgctatt cttttctcat
     4981 tccttgcttg gtttattcta tataaaactc gctttggatt aagattacgc tcagttggag
     5041 aacatcctca agcagcagac accttaggta ttaatgttta tcttatgcgt tactctggtg
     5101 tcttgatttc aggtttcctt ggtggaatag gaggagctgt atatgctcaa tctatttctg
     5161 ttaactttgc agcgacaact atattaggac cgggctttat ctccttagcg gcgatgattt
     5221 ttggtaagtg gaatccaatt ggtgcaatgc ttgctagtct attctttggt ttatcgcaaa
     5281 gtttagcagt tatcggtagc catttaccac ttttgagcaa tattccaaca gtttatctac
     5341 aaattgctcc atatgtcctc actatcattg tattagcagc tttctttgga caagcagtag
     5401 ctcctaaggc ggatggtatt aattatatta aaacgaaata aataaaaaga aactagtata
     5461 agctagtttc tttttattgt ttgtgaattt gtgaaaaaaa tactttgtga aaatttcaca
     5521 taaattataa tacaataaca ataaaatata aaaaaataga aaattgagtc atta
//
LOCUS       DATROD010000110         5511 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.112, whole genome shotgun sequence.
ACCESSION   DATROD010000110 DATROD010000000
VERSION     DATROD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.112"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..1448
                     /gene="fbsB"
                     /locus_tag="VBC74_001746"
     CDS             <1..1448
                     /gene="fbsB"
                     /locus_tag="VBC74_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibrinogen-binding surface protein FbsB"
                     /protein_id="HEO6942675.1"
                     /translation="SYRTVGTNEIAASVGIQGGFYQNFHDYYNYSYLLNSLWYEGNVK
                     EVVKDYENTIRQILSKKHEIEKILNQSTSDISIDDDDYEKGNKELLREKLNIILNLSK
                     RDYRVTPYYEVNKLHTGLILLEDVPNLKIAKDKLFSLENSLKEYKGEKVNYEELRFNT
                     EPLTSYLENKEKFLVPNIPYKNKLILREEDKYSFEDDEEEFGNELLSYNKLKNEVLPV
                     NITTSTILKPFEQKKIVEDFNPYSNLDNLEIKKIRLNGSQKQKVEQEKTKSPTPQKEA
                     VKEQTEQKISGKTQNVEKKSETVATPQQSSTAQTSVQQPAPVQPVVQESKASQEEINA
                     AHDAISAYKSTVNIANTAGVTTAEMTSLINTQTSNLSDVEKALGNNKVNNGAVNVLRE
                     DTSRLENMIWNRAYQAIEEFNVARNTYNNQMKSETVPVDNDIEAILAGSQAKISHLDN
                     RIGARHMDQAFVASLLEVTEMSKSISSRIKE"
     gene            1515..2060
                     /locus_tag="VBC74_001747"
     CDS             1515..2060
                     /locus_tag="VBC74_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942676.1"
                     /translation="MKKITTLILASSLLLVATTSVKADDNFEVPTRYVKMSEKSKAFY
                     QRLQEKQRKTHTTVKTFNNSEIRHQLPLKQEKARNDIYNLGILISQESKGFIQRIDNA
                     YSLENVSDIVNEAQALYKRNYDLFEKIKSTRDKVQVLLASHQGNTDLKNFYAELDDMY
                     EHVYLNESRVEAINRNIQKYN"
     regulatory      2211..2300
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2456..3022
                     /gene="thiW"
                     /locus_tag="VBC74_001748"
     CDS             2456..3022
                     /gene="thiW"
                     /locus_tag="VBC74_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEO6942677.1"
                     /translation="MTNHKTKKLTVLAIMIALDVFLTPIFRIEGMAFMSSVVNIIAGV
                     IMGPIYALVMALVTAFIRILTQAGVASVAPLAILGAGPGAFLAGWFYRFYAKDWMSWV
                     GEFIGTGIIGALLSAPIMVWFWTWTADGNHEVLAKASAAQSIFLFMPRFIGATIIGGV
                     IALLLLQGLKRVPVFMTIQALFKGEKVK"
     gene            3019..3573
                     /locus_tag="VBC74_001749"
     CDS             3019..3573
                     /locus_tag="VBC74_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEO6942678.1"
                     /translation="MKLGLKDVLLIALLAVVLGVVYFGAGYISNAFVPFVGPIAHEVI
                     YGIWFVAGPMALYILRKPGTAIVAELLAALIEVLIGSIYGPSVLVIGTLQGLGSELGF
                     TLFRYHNYKLPAFILSAILTSIFSFAWSFYANGLSAFSFSYNILMLIVRTVSSIIFFL
                     LTKNICDQLHRSGVLNAYGIHEGK"
     gene            3577..4863
                     /locus_tag="VBC74_001750"
     CDS             3577..4863
                     /locus_tag="VBC74_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6942679.1"
                     /translation="MLSVEKLAYTHGDSHYLFDEVTFSLNPGERILISGYSGCGKSTL
                     ALLLSGLKESGKGQVLLNGSLIEPSDVGFLFQNPDLQFCMDTVAHELYFILENLQIEP
                     EQMQDRSEFVLAQVGLKGFQNRLIYTLSQGEKQRLALATIFLKSPKLIILDEAFANLD
                     QESASQLLQLVLNYQANNQSMLIVIDHLITYYQDIMDHYFWLEKWLIRVDFDYMLNRL
                     NVFELEKKSHNTGDKLLSIKDFQVKLSKNKFISYLDFDLASGERLCLDGPSGVGKSSF
                     FMGLLGLYRTKGKKQFTHRKQIPISFLFQNPLDQFIFSTVYDEIFQVCKDSNKARDIL
                     ETINLWDKKQFSPFQLSQGQQRRLAIGSILASDSKLLLLDEPTYGQDAYHANMITTLL
                     LSYCHKNHCGVIFTSHDPHLKECFATRFLEVSSCSI"
     gene            4851..>5511
                     /locus_tag="VBC74_001751"
     CDS             4851..>5511
                     /locus_tag="VBC74_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEO6942680.1"
                     /translation="MFNLKNYHPAITFLCLLIPTFSFSFTLRLRTSLLFLIIVVTLQC
                     FVKVSLKTWAKVNLISFVMGLSLFLGTYFWGKLPHQFVLASLVACRPLIFMNVGLLFH
                     ASHSNYDFIESLYQTFKVPSHFAYGIFAVFNLLPLIKLQYQRNRLAFRLKNQVTWALS
                     PRLILSVLLKTIYWVEQLELAMLSKGFEVGKERTHASTYPVRFRDYSLLGMSILLAIG
                     MI"
BASE COUNT         1817 a          803 c         1051 g         1840 t
ORIGIN      
        1 aaagttacag aactgttgga acgaatgaaa tcgccgcttc tgtaggcatt caaggaggat
       61 tttatcaaaa cttccatgat tattataatt attcatatct attaaattct ttatggtatg
      121 aaggaaatgt aaaagaagta gttaaggatt atgaaaacac tattcgtcaa atactatcta
      181 aaaagcatga gattgaaaaa attcttaatc agagcacttc tgatatctct atagatgatg
      241 atgattacga aaaaggaaat aaagaattgc taagggaaaa attaaatatt attctaaatc
      301 tttcaaagag agattacaga gtaactccat actatgaagt gaataaacta catacagggc
      361 ttattttatt ggaggatgtc cctaatttaa agattgctaa ggataagttg ttttcattag
      421 agaattcttt aaaggaatac aaaggagaga aagttaatta tgaggaacta agattcaata
      481 cggaaccttt aactagttac ttagaaaata aagaaaaatt tttagtcccc aatattccat
      541 ataaaaataa attaatttta agggaagaag ataaatatag ttttgaagat gatgaagaag
      601 agtttggaaa tgaacttcta agttacaata agcttaagaa tgaagtttta cctgttaata
      661 ttacaacttc tactatatta aaaccgtttg aacagaagaa aattgtggaa gattttaatc
      721 cttattctaa tttagacaat ttagaaataa aaaaaataag gttgaatggc tcccaaaaac
      781 aaaaagtaga acaggaaaaa actaaatcgc caactcctca aaaagaggct gtaaaagaac
      841 aaactgagca aaaaatatct gggaagactc aaaacgtaga aaagaaatct gaaactgtgg
      901 caactccaca acaaagttca actgcgcaaa cttctgtcca acagccagct ccggttcaac
      961 cagttgttca agaatctaaa gcttctcaag aggagattaa tgcagcacat gatgccattt
     1021 cagcgtataa atcaacagtc aatattgcta atacagccgg tgtaacaact gcggaaatga
     1081 cctcgctcat taatactcaa acttctaatc tttctgatgt tgagaaagct ttagggaata
     1141 ataaggttaa taatggtgca gtcaatgtat tgagagaaga tacatctcgt cttgagaata
     1201 tgatttggaa tcgtgcttac caagctattg aagaattcaa cgtcgctcgt aatacttata
     1261 ataaccaaat gaagtcagaa acagttccag ttgataatga tattgaagct attttagcag
     1321 gttctcaagc taaaattagc catttggaca atcgtatcgg agcgcgccac atggatcaag
     1381 cttttgtagc tagtttatta gaagttactg agatgagtaa atcaatctca tcgcgtataa
     1441 aagaatagac actgctatca aggcgatctt aaacttttgt attgagctaa cctaaaagat
     1501 agaaagagac taatatgaaa aaaataacaa ctttaatctt agctagtagc ttattactag
     1561 ttgcaacgac atcggttaaa gctgatgata actttgaagt accaacgcgt tatgttaaaa
     1621 tgagtgaaaa atcaaaagca ttttatcaaa gattacaaga aaaacaacgt aaaacacata
     1681 ctactgtgaa gacttttaat aattcagaaa taaggcatca actacctctt aaacaagaaa
     1741 aggctaggaa tgatatctac aatttaggca ttcttatttc tcaggagtct aaagggttca
     1801 tccaacgtat tgataatgcc tattctttgg aaaatgtctc agatattgtt aatgaagctc
     1861 aggctttgta taaacgcaac tatgatttat ttgaaaaaat caaatctaca cgtgataagg
     1921 ttcaagtctt acttgcatcg catcaaggta atacagattt aaaaaacttt tatgctgagt
     1981 tggacgatat gtatgaacat gtttatctca acgaaagtag agtggaggcg ataaacagaa
     2041 atatccaaaa atataattag tttctaaact aacaaacatt cctaaatata agacattaaa
     2101 ccctacttat tgattagtga gtagggtttt actgttttaa atagctttct gctcagaatg
     2161 taagccttgt catttcaaag gaactatgtt attattctta agtaaattaa ataggacatt
     2221 tggggtgcgt aacagctgag attataccca ttgaacttga tacagttagt actgtcgaag
     2281 ggaaatgtgt gccattacaa gttttttgta atggaaaagg gcgtagtgtc ttcttaggag
     2341 acagctagct ctagttaatc agataataaa ctccgtttgc ttgcagatgg agttttttgt
     2401 cgtcaaaatt ttttataggt tgttgcccct tatctgtcat agagaaggag ttatgatgac
     2461 taatcataaa acaaaaaaat taacggtttt agcaattatg attgctttag atgtttttct
     2521 aacgcccatt tttagaatag aaggcatggc ctttatgtct agtgtggtta atattatcgc
     2581 tggagttata atgggaccaa tctatgcatt agttatggca cttgtaacgg cttttattcg
     2641 tattttaact caagcaggag tagcatctgt tgcaccttta gctattctag gagctggtcc
     2701 aggagctttc ttagcgggtt ggttttatcg gttttatgcg aaagactgga tgtcttgggt
     2761 gggtgagttt ataggaacag ggataattgg agcactttta tcagctccaa ttatggtctg
     2821 gttttggact tggacagcgg atggaaatca cgaagtgtta gctaaagcat ctgcagctca
     2881 atccattttt ctttttatgc caaggtttat aggggcgact ataattggcg gtgttattgc
     2941 tttgttgcta cttcagggat taaagcgggt tcctgtattt atgacaatac aagcattatt
     3001 taaaggagaa aaagtaaaat gaaattgggt ttaaaagatg tattattgat tgctttatta
     3061 gcggttgttc tgggcgttgt ttactttgga gcaggctata tttcaaatgc ttttgtacct
     3121 tttgtaggtc ctatagcgca tgaagttatt tatggtattt ggtttgtggc aggtccaatg
     3181 gctctttata tattgaggaa accaggaacc gctattgtag cagaattgtt agctgctttg
     3241 atagaggttt taattggatc aatttatgga ccaagtgtac tcgttattgg tacccttcaa
     3301 ggtttagggt cagaattagg ctttacgctg tttaggtatc ataattacaa gttgccagct
     3361 tttatattgt ctgctatttt gacatcaatt tttagttttg catggagttt ttatgccaat
     3421 ggacttagtg cattttcatt ttcatataac attctgatgc ttatagtaag aactgtttca
     3481 tctattattt tcttcttatt aacaaagaat atatgtgacc aattgcatag aagtggtgta
     3541 ctgaatgctt atgggattca tgaagggaag tagcctatgc tatctgttga aaaattagct
     3601 tacacccatg gcgatagtca ttatttattt gatgaagtga cttttagtct taacccagga
     3661 gaacgaattt taattagtgg ctattcaggg tgtggtaaga gtacattggc tcttttacta
     3721 tcagggttaa aagaaagtgg gaaaggtcaa gttttattaa acggctcatt aatagaacca
     3781 agcgatgtgg gttttctttt tcaaaatcct gatttgcaat tttgtatgga tactgtggcg
     3841 catgagttgt attttatttt agaaaattta caaatagaac cggagcaaat gcaagataga
     3901 agtgagtttg tattggcaca agttggttta aaaggatttc agaatcgttt gatttatact
     3961 ttatctcaag gtgaaaaaca gcgattagca ttggcgacta tttttttaaa atctcctaaa
     4021 cttattattt tagatgaagc ttttgcgaat ttggatcaag agagtgccag tcaactatta
     4081 caattagttc ttaattatca agcaaacaat caatcaatgc tgattgtaat cgatcatttg
     4141 attacttatt atcaagatat aatggatcac tatttttggt tggaaaaatg gttgataagg
     4201 gtcgattttg attatatgct caatcgtttg aatgtcttcg aattagaaaa gaaaagtcat
     4261 aatacaggtg acaaactgtt atcaattaaa gatttccaag ttaaactttc aaaaaacaaa
     4321 ttcatctctt accttgattt tgatttagca agtggggaga ggttgtgctt agatggacca
     4381 agtggcgtgg ggaagtctag tttctttatg ggattgttag ggctctatag gactaaagga
     4441 aagaaacaat ttactcatag aaagcaaatt cctataagct ttttatttca aaatccctta
     4501 gatcaattta ttttttcaac agtttatgac gaaatttttc aggtttgcaa ggatagtaac
     4561 aaagcgaggg atattttgga aaccataaat ctttgggata aaaaacagtt ttcaccattt
     4621 caactaagtc aaggccaaca gaggcgatta gccattggca gtattttagc tagtgatagt
     4681 aaacttctat tattagatga accaacttat ggacaagatg cctatcacgc taatatgatt
     4741 acgactcttc ttttgagtta ttgtcacaaa aatcattgtg gggttatttt tactagccat
     4801 gatcctcact taaaagagtg ttttgcaaca cgtttcttgg aggtgtcatc ttgttcaatt
     4861 tgaaaaacta tcatccagct atcaccttcc tatgtttatt aataccaacc ttttcgttta
     4921 gtttcacatt gaggctaaga acgtctttgt tatttctaat tatagtagta acactgcaat
     4981 gcttcgtaaa ggtttcttta aaaacttggg cgaaggtcaa tttgatctca tttgtgatgg
     5041 gactctcttt atttttaggg acgtattttt gggggaaatt accccatcaa ttcgtcttag
     5101 ctagtctagt ggcctgtcgt ccgcttattt ttatgaacgt gggtttgtta tttcatgcat
     5161 cacattctaa ttatgatttt atagaatctc tttatcaaac tttcaaagtg cctagtcatt
     5221 ttgcatatgg tatttttgcg gtctttaatc ttttaccatt aattaaatta caataccaac
     5281 gtaatcgact cgcattccga ctgaagaatc aggtaacttg ggcattatcg cctagactaa
     5341 tattgtcagt tttattaaag acgatatatt gggtagaaca gttagagtta gcaatgcttt
     5401 ctaaaggttt tgaagtgggt aaagagcgaa cacatgcttc aacttatccc gttcgttttc
     5461 gagattatag tttacttggt atgagtattt tattggctat aggaatgatt t
//
LOCUS       DATROD010000111         5501 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.113, whole genome shotgun sequence.
ACCESSION   DATROD010000111 DATROD010000000
VERSION     DATROD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.113"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            133..5458
                     /locus_tag="VBC74_001752"
                     /pseudo
     CDS             133..5458
                     /locus_tag="VBC74_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822652.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
BASE COUNT         2019 a          882 c         1044 g         1556 t
ORIGIN      
        1 aatctaaata agataaaaat tcttaaaaaa tactcattaa ttagcaaata gatattgaca
       61 actctataca aaagtgctta aattaagctt gcttaacaaa taaaataatc taacttaaaa
      121 atgagtggaa ttatgaaaag aatttcttta aaaacaaccg gtgtactgat tgcgttttta
      181 gctgtaaatg cggtagcgac ctctgtagtg gtagatgcag acacagtagt ccctcatact
      241 agtgcgaata ctagtcagct tttagaaaaa aacacaacta tcaagccttc cgaagttaaa
      301 cagtcacgta ctgaaagtaa tgcagtaacc cctccaacaa tagcctcgag taactcacaa
      361 aagcttgaga ataaatcatc tgtagattca aatatactaa agagtgaaca acctgcacat
      421 aaagttgctg gtgttaaaca agtcgttcaa gaacaaacga aaaatcaaaa agatgaaacc
      481 tctttacagg ctaaaaagca agtacaatca agtgaaaagt tattaaatac ttcatcacaa
      541 ccttcaagtg aggaaaacaa aaacactaat aagcctaata aggctaatga agtggatatg
      601 aaaaatgtcg tgaataatgc ttcgacgaag ccaactcccc catctgattc taaagtgtat
      661 aagcgcgtta ttgtaacatt tgatcaagta aataaggaaa aagtagatga agcccttaaa
      721 gcaattccgg gcactcgtat taagttaact tatagtagcc tttttaatgg ctattcatta
      781 gcagtagatg aaaaagaaat tgaaaaaatt ggtaagatta aagagataaa acatattgaa
      841 gcagttcaag tattaaaacc tcaaatgttt actgctaacc aatggacaca tgtcttagct
      901 gcacgtttaa aagctaaaca aaatagtaaa gttgacggta gagggctact tattgcgact
      961 attgatagtg gtgtagatat ccatcatcct gccatgcgat tggatgagaa tgatccagat
     1021 gtgatggcaa agcgtcgtat taaagatatt aaggctcctt atacaaataa agttcctttt
     1081 gcatacaatt atcaacgagg cgataattta gaattgaaag acgacgttca gcgccctcat
     1141 ggtatgcatt tagcaggtat cttagcggga aatgcaaagg atggttttaa aggagttgcc
     1201 cctaatgcac agttattagt gtacaaaatt tcaacaaata gagaagatcc atctgcagag
     1261 tggaccggct ctgatgcagt cttccatgca atggaagatg ccgctaaaag aggggcagac
     1321 gttattagtt taagctttgg atatttggga acaggacgtc caggtgaact ttggtatgag
     1381 acaattaaga aggtgactga caagggtgtt atcgtagtag cagcaatggg aaactatggt
     1441 aattcggctt catctactac ctacgataat gtcgttgaca actctttggg aacaaaagat
     1501 actgctgcta tggtgtctgt tgcagctgtt gataaggtta ttggagttgg ttcagttcaa
     1561 aatagtgctc ttcttttaga tgttttgaaa gttggaaatc ttaaattagg atatgcagaa
     1621 ctgggacata aaagtcaaac aatattaaaa atgctcaaaa ataaaacatt tgatctagtc
     1681 tatgttggta aaggccagtt gaaaaaagat atctcagaag agtaccttaa aagttatact
     1741 ggaaaagtta tcgtcgttga gcgtggagga gagggcgtta aagataagct aaaacgtttt
     1801 atcggaattg ctaaaggggt tattatgata aatgaacctg cttattatag ttctgggaat
     1861 tttgatactc accccatgtt tgattatcac tatgatatac cagaaggtaa atgggctatt
     1921 agtctatcaa aaaaagatgg tgatcagttg attgactaca tcaagaaaaa caaaactgct
     1981 aaattagaat ttttacctaa agaaaccttt gttcgccgtc caaatcctac aatttcaggc
     2041 ttttctagtt ggggagtgac accagatttg catttgaagc cagatgtagt tgctccagga
     2101 gaagatattt attctacttt taacgacggt acctatgggt acatgtctgg aacttcaatg
     2161 gctacccctc aagtagctgc cattagtgca ttagtaaaaa gtaaagtagc tgaaataatg
     2221 gcattaaaac ttccaatgtt agctggtttg acaaacgttg atttgacaaa gattgtcttg
     2281 atgaatacaa gtcaaccttt gaaagatact ctaaataaag ctttagaaat ttcaccacga
     2341 cgtcaaggtg caggtatggt taacttagaa aaagctttag ctaacgatgt tcttgttttt
     2401 ggtgataaaa caggatcatt ctctataaaa gagattaaga accataaaag atttaccttg
     2461 aaattggtca atacaggaca aaaacgtcgt aactttagta ttgaaccagg agcgatttta
     2521 acaactaaaa ttgttgataa agtgactaat caggggtatg aaaatgtcgt tttaaaggca
     2581 gttcacgcta aagtaataga gggagcaact ttacaaatgg gtaaagttat ctcactagca
     2641 ccgcaatcag ccatagagct tgatatagat cttgagataa aaaaacctat acaagatttt
     2701 gctgaagggt atttgtattt caaaacttta gataaagatg gacaagatat ctcagctcct
     2761 tacatgggct tctcaggaac atgggattaa gaaccaattt ttgacgcacc tatgtgggat
     2821 agtcgttcta aaaataaatt aacgaccttg atgcaagcaa ctttggttgg acacggaact
     2881 aacaaatttt atgaaatcgg acaagaaata ggtagtaaaa aggtcaatcc atccctattt
     2941 actatgtcta atgtgcgtgt agctcccgat aaagttgtgc gtgtagcacc tcgattttct
     3001 ttattacgct cagcatcaga ttttgacctc tctattgtat cagaagcaaa agacgattca
     3061 ccaatacttc gtcgtataac agtgaaacat ttcgtggaga aatattacaa atcaagaaat
     3121 gaggaagaag actcatatta tgatgctttg agaacgacag aatcatcgct agtttgggat
     3181 ggaaagtttt atgatcctaa gactggtgat tttaaagctg caaagccagg cttatattac
     3241 tttaagttac gtgcacgttc aaatcctaca gatccttatc aagtccaata ccttccaatt
     3301 ttaatagata atgaaagacc aactttttct cttaaaaaag ttaataacgg ctttgatatt
     3361 ttaacccatg ataatcatcg tgttaaagaa gtaattgttc gtaataaaga gacacaaaag
     3421 cctatcacag ttaaaaaaat tgccgaaaat cactatcata ttgatgagtt aacgttaaga
     3481 gaatctgatc tggatataaa agttgcagac tatgctggga attcaaacaa ggaaattgct
     3541 ttatcagaat cagaagaagt atcagagaaa tcttactcta gtgaggaaga taatacatta
     3601 caaccacagt cactgtctca tcgcaatatg aaagctcaag caaccagttc agatgatgac
     3661 gatatctggg atgataacga tgatgacctt aacgaagaaa aagattttga ttttgaagaa
     3721 aatatttctg atatcgagga tggtttatta ttaactcgag aaacggtata ttcaattcga
     3781 actgtaaata aaattcgaaa tgctgaaaat gaaagcttgg agcatagtgt ttctctctta
     3841 ttaaaagatg gctatcgtgc agaaataaga gttgttaacc ttcatgagaa cgctaaagat
     3901 ccaaaacatt ccgacccata tcgtacagta tccaacccta tttatatgta tggtgatgac
     3961 tctgacacat ttaaaaccac atgggtcaaa tttatgtctg ggaataacat catgaatttg
     4021 aaggtttata ataagaatga taaattggta tttagtcgtg gctatcatat taccgttgat
     4081 gtacaacctc caaaattaga actggacaag actaacatag aagaagctgc tgacaaagat
     4141 aactttatca ctggtcaaat ctatgctcaa aatggagaaa ttgaaatcaa agggcacgtt
     4201 tctgataatg taaaaggttg gggtctttct attaatggtg atagcgtcga tgttaaatat
     4261 tcaagtggtg aatttatcag taatagatga gcatttactc ataagataaa agcagcagca
     4321 ggagatattg ttacgatttc agcggtagat acatcaggta ataccgtttc tgaacattat
     4381 aaggtagtta atgatgtgga tacaccgaag ttaactatta atgaagcaga tttattatcc
     4441 ggaaaagtca aagtggttat agctaaagca gaagataatc accttaaaaa tttaacgata
     4501 actgttaatg gcaagcccta cacagaagga caaagtattg actcttacca attaaaaggt
     4561 ttcgaagggc gttacgtcat caaagtatca gcagttgata aatcaggtaa agtcactgaa
     4621 aaaattataa caatcggcaa cttccctgaa ttatcagcaa gtttacgcaa aacctctttc
     4681 caaatgcaag atcttacctc tatcgataat ttattatccc tagcccacaa tcagactgcg
     4741 gaattgatta agccagtcca actcatggac aaaaaagtga gctttacggc aaaaatatct
     4801 gacccccttg gtcgttctgt ggttagagag tttgaagcat tattatcaac ttcaaacctt
     4861 aaagtagatt caaaactaaa accacatttg aaaaaacaaa ctctcctttt aacagatcca
     4921 aaaggaatta cggacctttt ggatctgtta aaaggagagc tctattttat tgacagaaat
     4981 agtcgataat aaagtgggaa atcatactat aaagggtttg atatttaaaa atggtgcata
     5041 taaccatttc gaaacaagta tacaaattgt aaaaacaatg gaagaaatca actctgacag
     5101 acgtttgaaa aatgatcata aaagctctga cgacacaaaa ctaaaaaaag aaacagagtc
     5161 gttaaaaggg cataaggttg atccagcagt tcatggtgaa tttaaagaaa tatctaaaca
     5221 tcaaatggat aaaccacaag tgatagctgt aggcatgaaa gggagtacaa agacaccccc
     5281 atttgttcat agtcaaatgg aagcgaagaa aacaactaag attccaatgt ctagagcggc
     5341 aaaacatgaa ttgccaagga caagtgatga caataataat ttactggcta aaggaattgc
     5401 actcatagta attgtacttg gtttactagg ccttaaaaaa gcttcaaaga aatattgatt
     5461 ttgattaaat tagttttcta atttattaag ctaaaattcc t
//
LOCUS       DATROD010000112         5450 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.114, whole genome shotgun sequence.
ACCESSION   DATROD010000112 DATROD010000000
VERSION     DATROD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.114"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            142..561
                     /locus_tag="VBC74_001753"
     CDS             142..561
                     /locus_tag="VBC74_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEO6942681.1"
                     /translation="MDNCIFCKIISGEISSSKVYEDDEVLAFLDITQTTTGHTLLIPK
                     KHVRNVLEMDEKTAQITFERLPKVARAVQAATKAKGMNIINNNEEIAGQTVFHAHVHL
                     VPRFDESDGIKIHYTTHEPDFEALAKLAKEIRKEIQL"
     gene            558..854
                     /locus_tag="VBC74_001754"
     CDS             558..854
                     /locus_tag="VBC74_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942682.1"
                     /translation="MKIGRLIALGLVSLGAFELYKNRKTIKDSYQNTKNETDSAKLKL
                     ERIKNDLAIISQEKEKIRLISQELNHKFQVFNKDIQPRLEEINQRMAKYQEKDE"
     gene            complement(918..2225)
                     /locus_tag="VBC74_001755"
     CDS             complement(918..2225)
                     /locus_tag="VBC74_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO6942683.1"
                     /translation="MKIWKKITLMFSAIILTTVIALGVYVASAYNFSTNELSKTFKDF
                     KLAKSKSHAIEETKPFSILLMGVDTGSEHRKSKWSGNSDSMILVTINPKTNKTTMTSL
                     ERDVLIKLSGPKNNGQTGVEAKLNAAYASGGAEMALMTVQDLLDINVDYFMQINMQGL
                     VDLVNAVGGITVTNKFDFPISIAANEPEYKAVVEPGTHKINGEQALVYSRMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALNSISSYKKILSAVSNNMQTNIEISSKTIPNLLAYKD
                     SLEHIKSYQLKGEDATLSDGGSYQILTKKHLLAVQNRIKKELDKKRSKTLKTSAILYE
                     DYYGTTASNDSSTYSSTQENNYNTTPYSEAPPSYSGNTTYSSETNQTTHQSYYNSSTP
                     ASNYSSNTNTGQADSSGSVNNYNGAATPNPNTGTQPVPSQTNP"
     gene            complement(2234..2794)
                     /locus_tag="VBC74_001756"
     CDS             complement(2234..2794)
                     /locus_tag="VBC74_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001890427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6942684.1"
                     /translation="MGIAQEKLLRRFKMSKQFAVDFIEASREEASALLEFLNTVTEET
                     DFILHTVSNQLSLPEMETFIENTLITKNCICLIAKLKNKVIGLITIISQSDIEIEHIG
                     DLFIAVQKDYWGYGIGHILMEEAIEWASDNDITRRLELSVQGRNERAIHLYQKFSFEI
                     DGLQTRGIKRENGEFLDIYRMSKLID"
     gene            complement(2748..3257)
                     /gene="tsaE"
                     /locus_tag="VBC74_001757"
     CDS             complement(2748..3257)
                     /gene="tsaE"
                     /locus_tag="VBC74_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEO6942685.1"
                     /translation="MLWGCQNQQTVGRQRYFWYNISMFYTQNEEELIALGQKLGTVLK
                     SGDIVLLTGNLGAGKTTLTKGIAKGLDIKQMIKSPTYTIVREYEGRVPLYHLDVYRIG
                     DDPDSIDLDDFLFGQGVTVIEWGELLSDNLINNYLEIVITRSNQGRQVQLEAYGHRAR
                     EIIEAIQDV"
     gene            complement(3340..4761)
                     /locus_tag="VBC74_001758"
     CDS             complement(3340..4761)
                     /locus_tag="VBC74_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEO6942686.1"
                     /translation="MEKFFKLKEHGTTIRTEITAGLTTFFAMSYILFVNPAILSQTGM
                     PAQGVFLATIIGAVVATSVMAFYANLPYAQAPGMGLNAFFTYTVVFALGYTWQEALAM
                     VFICGLISLIITLTKVRKMIIESIPTTLKSAITAGIGTFLAYVGIKNAGFLKFSIDPG
                     TYDVVGKGAAKGLATITANSSATPGLVSFDNPAILLSLIGLSITIFFIVKGIRGGIIL
                     SILTTTLLGILMGVVKLDAINWEATNLSASFRDLKQVFGVALGEKGLISLFSNPSRLP
                     SVLMAILAFSLTDIFDTIGTLIGTGEKVGILATTGDNHESKSLDKALYSDLIGTTFGA
                     ICGTSNVTTYVESAAGIGAGGRTGLTALVVAGLFAISSFFSPLVSIVPSQATAPILVI
                     VGIMMLSNLKDIKWDDMSEAIPAFFTSLFMGFTYSITYGIAAGFLTYTLAKVIKGQAK
                     DIHVVLWILDILFILNFISLAIL"
     gene            complement(4864..>5450)
                     /locus_tag="VBC74_001759"
     CDS             complement(4864..>5450)
                     /locus_tag="VBC74_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HEO6942687.1"
                     /translation="LFFIVISFGIKYYHLQGPNLIWNMTLALIALDFAYLTSLFKKKI
                     LIGLFALAWFFFYPNTFYMLTDIIHMHFVGDVLYNKTNLILYILYVSSILFGFLSGIE
                     SFSVIMRKFRISNIFLRWGIIGIVSFVSSFGIHIGRYARLNSWDILTKPQVVINELLA
                     VPSRDSFHFILGFTFLQVLCLIFMDYSAGKKLEK"
BASE COUNT         1883 a         1029 c          811 g         1727 t
ORIGIN      
        1 aagtatgatg gtaaatatag atatcacaat caatacaggc tacttttatc cacctgtacg
       61 acttatgacc tatcaaacga taaaaattta ccattcctta atagctaccc aataaatttt
      121 tcaacatgaa aggaattagt tatggataat tgtatttttt gtaaaatcat cagtggagaa
      181 atctcttcat caaaagttta tgaagatgat gaagtattag cttttctcga cattacacaa
      241 actacgactg ggcacaccct tcttattcct aaaaaacatg ttcgaaatgt gttagagatg
      301 gatgaaaaaa ctgcacagat aacctttgaa cgcttgccta aagtggcgcg tgctgtccaa
      361 gctgctacaa aagccaaagg aatgaatatt attaacaaca atgaggaaat tgctggacaa
      421 actgtttttc atgcacacgt ccaccttgtt ccccgctttg atgaatcaga cggtatcaaa
      481 attcactaca ctactcatga acccgacttt gaggcacttg caaaattagc caaagaaatt
      541 agaaaagaga ttcaattatg aaaataggtc gtttaattgc actaggttta gttagtttag
      601 gagcgtttga actttacaaa aatagaaaaa ctatcaagga ttcctatcaa aatacaaaaa
      661 atgagacaga ttcagctaaa ttaaaattag aacgtatcaa aaatgatctt gctattatta
      721 gtcaggaaaa agaaaaaatc agattaattt ctcaagaact caatcataaa ttccaagtgt
      781 ttaataaaga tattcaacct cgtttagaag aaattaatca acgcatggct aaatatcaag
      841 agaaagacga ataagccgac ggtataaaaa tatctactct aaatataaaa agagcaaata
      901 atgctctttt ttatatttta tggattagtt tgacttggta ctggttgcgt tcctgtgttt
      961 ggattaggcg ttgcagcccc gttataatta ttaacacttc cacttgaatc agcctgacct
     1021 gtgttagtgt tactgctata gttactagca ggagtgctac tattatagta actttgatga
     1081 gttgtttgat tagtctcaga actataagta gtattaccac tgtaacttgg tggtgcttct
     1141 gaataaggtg ttgtattata attattctct tgtgttgatg aataagtaga agaatcatta
     1201 ctagcagtag taccatagta atcttcatat agaatcgcgc ttgtcttcag agttttacta
     1261 cgctttttat ccagctcttt cttaattcta ttttgaactg caagtagatg tttcttagtt
     1321 aaaatttgat aagagccacc atctgataga gtagcgtctt cacccttcaa ctgataagat
     1381 ttaatatgtt ccaatgaatc tttataagct aacaaattag gaatcgtttt tgatgatatc
     1441 tcaatattag tttgcatgtt attacttact gcggaaagaa tttttttgta tgaactaata
     1501 ctatttaacg ccaatatttt tttaaggact ttttgaatta cttcacgttg tcttttttga
     1561 cgcccataat ctccctctgg atcatcatag cgcatacgag aataaacaag tgcttgttct
     1621 ccatttattt tatgtgtccc tggttcaaca acagccttgt actctggttc attggcagca
     1681 attgatattg gaaagtcaaa tttattagtt actgttatac caccaacagc attgaccaaa
     1741 tcaactaatc cttgcatatt aatttgcata aagtaatcaa cattaatatc taataagtct
     1801 tgaacagtca tcaatgccat ttccgcacca ccagaagcat aggctgcatt tagctttgct
     1861 tctacgccag tctgtccatt atttttggga ccactcaatt taatcaatac gtcacgttct
     1921 aagcttgtca tcgttgtttt attagtttta ggatttatag tgactaagat catagaatcg
     1981 ctatttcctg accacttaga ttttcgatgc tctgaacctg tgtccacacc cattaatagt
     2041 attgaaaacg gctttgtttc ttcaatagca tgactttttg atttagctag cttaaaatct
     2101 ttaaaagtct tagacaattc attagtcgaa aaattataag ctgaggcaac atagactccc
     2161 aatgcaatta ctgttgttaa aataattgca gaaaacatta aggttatttt tttccaaatt
     2221 ttcatgtata ttcctaatct atcaacttac tcattctgta aatgtctaga aattctccat
     2281 tttctctttt aatacctctt gtttgaagcc catctatttc aaaactgaat ttttggtaga
     2341 ggtgaatagc acgttcatta cgtccttgga ctgatagttc taagcggcgt gtaatatcgt
     2401 tatctgacgc ccattctatt gcttcttcca tgagaatatg accaatacca tacccccaat
     2461 aatccttctg aacagcaata aaaagatccc caatgtgttc aatttcaata tcagactgac
     2521 taatgattgt tatcaagccg atcactttat tttttaattt agcaatcaaa caaatgcaat
     2581 ttttcgttat taaggtattt tcaataaacg tttccatttc tggcaaagat agttgattgc
     2641 tcaccgtatg caaaataaaa tctgtttcct cagtaacagt atttaaaaat tcaagaagcg
     2701 cagatgcttc ctctctcgaa gcttctataa aatcaacagc aaattgttta gacatcttga
     2761 atcgcctcaa taatttctct tgcgcgatgc ccataagctt ctaactgaac ttgacgtcct
     2821 tgattgctcc tcgtaataac aatttctaag tagttattga taaggttatc actaagcaac
     2881 tctccccatt caataactgt tactccttga ccaaacaaga agtcatctaa gtcaatagaa
     2941 tcaggatcat ctccaatacg ataaacatcc aaatggtaaa gtggtacacg cccttcatat
     3001 tctctaacaa tagtgtaagt tgggctctta atcatttgtt ttatgtctaa tccctttgca
     3061 ataccttttg taagagttgt tttgcctgca cctaaattac cagtcaataa tacgatatct
     3121 cctgacttta aaacagtacc cagtttttgc cctagggcaa taagctcttc ttcattttga
     3181 gtataaaaca tagagatatt ataccagaaa tatctctgtc ttccaacagt ttgctgattc
     3241 tgacagcccc atagcatata tttcttcact tttcttaaga aaaaaggaaa ccaggctaca
     3301 aatctgattt ccttttatct tatttatcaa actataatat tacaaaatag ccaagctgat
     3361 aaagtttaga ataaataata tatctaaaat ccacagtacc acatgaatat cttttgcttg
     3421 tcctttgatg actttagcta gtgtataggt caagaaacca gctgcaatac cataagtaat
     3481 gctataagta aaccccataa aaagagaagt aaagaaagct gggatagctt ctgacatatc
     3541 atcccatttg atatctttta aatttgaaag catcataata ccaacaataa ccaatattgg
     3601 tgcagtagct tgactcggaa cgatagatac tagtggacta aaaaaactag atatagcaaa
     3661 caaaccagca actactaagg ctgtcaatcc tgtacgacca ccagctccga ttcctgctgc
     3721 tgactcaaca taggttgtaa cgttagatgt tccacaaata gcaccaaaag ttgtcccaat
     3781 taagtctgaa tagagtgctt tgtctaaact cttagattca tgattatcac cagtcgttgc
     3841 taagatacca actttttctc cggtaccaat gagcgttcca atggtatcaa aaatatcagt
     3901 cagtgaaaaa gcaagaatgg ccattaatac acttggtaga cgtgaaggat tagaaaataa
     3961 agaaattaat cctttctctc ctaatgcaac tccaaatact tgcttcaaat ctctaaaaga
     4021 ggccgagagg ttagttgctt cccaattaat agcatctaac ttaaccactc ccattaaaat
     4081 acctaagagg gtcgttgtta atatcgatag aataatccct ccacggatac cttttacaat
     4141 aaagaagatg gtaatgctta aaccaatcaa agataataaa attgctggat tatcaaacga
     4201 cactagccct ggcgttgctg aagaatttgc tgtaatagta gccaatcctt tcgcagcacc
     4261 ctttccaaca acatcatacg tcccaggatc aattgaaaat ttcaaaaaac cagcattttt
     4321 aatcccaacg tatgctaaaa aagttccgat tcctgctgta atggctgatt ttaaagttgt
     4381 tgggatagac tcaataatca tctttctaac tttcgttaaa gtaataatca gagaaatcaa
     4441 accacaaata aataccatag ctaaggcttc ttgccatgta tatcccaatg caaagacaac
     4501 tgtatatgta aagaaagcat ttaatcccat cccaggagct tgtgcatagg gaaggttagc
     4561 atagaaagcc ataacagatg tagctacaac ggcaccaata atcgtcgcta agaaaactcc
     4621 ttgtgctggc atcccagtct gagataaaat agctggattt acaaacaaaa tgtacgacat
     4681 tgcaaagaaa gtcgttagac cagcagtgat ttcagtccga attgtcgtcc cgtgttcctt
     4741 tagcttaaaa aacttttcca ttttaaaatc ggattctcct ttattttttg ttattttaca
     4801 cttttaaaaa aattaatcgt tcgtatattt acgaactatt aatatatatt aacaaaaaat
     4861 aattcacttt tcaagctttt tccctgcaga ataatccata aaaataagac acaatacctg
     4921 taaaaatgta aatcctaaaa taaagtggaa tgaatcccta ctcggaaccg ctaaaagttc
     4981 gttaattacc acttgtggct tcgttaagat atcccatgaa tttagacggg cataacgacc
     5041 aatatgaata ccaaaacttg aaacaaatga tacaattcca ataattcccc atcttaaaaa
     5101 tatattgcta attctaaatt tacgcataat cacagaaaaa ctttcgatac ctgataaaaa
     5161 accaaacaag atactagata catacaagat ataaagtatt aaatttgtct tattataaag
     5221 aacgtctccg acaaaatgca tatgaatgat atccgtcaac atataaaagg tattaggata
     5281 gaaaaaaaac caagcaagtg caaaaagacc gataagaatt tttttcttaa ataaactagt
     5341 aaggtatgca aaatctaacg ctattagggc tagagtcata ttccaaatca aatttggtcc
     5401 ctgcaaatga taatatttaa tcccaaaaga aataacaata aaaaataaat
//
LOCUS       DATROD010000113         5393 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.115, whole genome shotgun sequence.
ACCESSION   DATROD010000113 DATROD010000000
VERSION     DATROD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.115"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            137..1303
                     /locus_tag="VBC74_001760"
     CDS             137..1303
                     /locus_tag="VBC74_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEO6942688.1"
                     /translation="MEKRLSLGALVLASTVLLAACGNVGGGASSTGTKIGKDIKVGYN
                     WELSGNVSSYGNSMKNGADLAVKEINAAGGVGGKKLKVLSQDNKSENAEAATVATNLV
                     TKGANVIIGPATSGAAASSTPKVNAAAVPMIAPAATQDNLVYGSDGKTLNQYFFRATF
                     VDNYQGKLLSQYATDNLKAKKVVLFYDNSSDYSKGVAKSFKESYSGKIVDSMTFSAGD
                     TDFQASLTKLKGKEYDAIVMPGYYTETGLIVKQARDLGISKPVLGPDGFDSPKFVQSA
                     TPVGASNVYYLTGFTTQGSTKAKAFHDHYVKAYGEEPSMFSALSYDAVYMAAKSAKGA
                     KTSIDIKKALAKLKDFKGVTGKMSIDKNHNVVKSAYVVKLEDGKTSSVNIISAK"
     gene            1409..2278
                     /locus_tag="VBC74_001761"
     CDS             1409..2278
                     /locus_tag="VBC74_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO6942689.1"
                     /translation="MLQQLVNGLILGSIYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FMGYYLINHLHLNFFLALLIAMLGSAFLGVVIEYLAYRPLRKSTRIAALITAIGVSFL
                     LEYGMVYLVGADTRAFPQAIHTVKYNLGPITITNVQLIILGIALLLMLTLQFIVQKTK
                     MGKAMRALSVDSDAAQLMGINVNRTISFTFALGSALAGAGGVLIGLYYNSVQPLMGVT
                     PGLKAFVAAVLGGIGIIPGAAIGGFVIGILETLATALGVSDFRDGIVYAILILIFLIR
                     PAGILGKNIKEKV"
     gene            2281..3234
                     /locus_tag="VBC74_001762"
     CDS             2281..3234
                     /locus_tag="VBC74_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEO6942690.1"
                     /translation="MKENMKSILSWLAIVVLGYLLISVLISMGIFNLYHIQIIETIGI
                     NVILAVGLNLIVGCSGQFSLGHAGFMAIGAYAVAIIGVKMPTYVGFLIAILVGTLVAG
                     GIALGVGIPTLRLKGDYLAIATLGVAEIIRILLVNGGDITNGAAGIMGIPPFTTWSLV
                     YGVAVVSLILAMNFLRSPLGRNTIAIREDEIAAESMGVDTTKVKVIVFVFGAILASIA
                     GSLQAGYVGTVMPKDFSFMMSVNVLIIVVLGGLGSMTGTVLAAILLGLLNMLLQDYAS
                     VRMIIYALALILIMIFRPSGLLGTKELTLSHLFRKTSKGGN"
     gene            3235..3999
                     /locus_tag="VBC74_001763"
     CDS             3235..3999
                     /locus_tag="VBC74_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6942691.1"
                     /translation="MALLEVKNLSKHFGGLTAVGDVSMKLHKGELIGLIGPNGAGKTT
                     LFNLLTGVYLPSKGTISLDGKILNGRKPAKIASLGLGRTFQNIRLFKNMTVLDNVLVG
                     LSNHHLSHPIASFLRLPKYYHSEKALRKKALELLEIFGLKAYQDALAKNLPYGKQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTQLISQIKSDFDITIMLIEHDMNLVMQV
                     TERIYVLEYGRLIAHGTPEEIKNNKRVIEAYLGGEL"
     gene            3999..4709
                     /locus_tag="VBC74_001764"
     CDS             3999..4709
                     /locus_tag="VBC74_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6942692.1"
                     /translation="MTMLKVENLSIHYGVIQAVNDVSFEVNQGEVVTLIGANGAGKTS
                     ILRTISGLVRPSQGSISFMGKPIHKLAARKIVGNGLAQVPEGRHVFSSLSVMENLEMG
                     AFLQKDREQNQKMLKKVFDRFPRLEERKNQDAATLSGGEQQMLAMGRALMSRPKLLLL
                     DEQSMGLAPIFIQEIFNIIEDIKKQGTTVLLVEQNANKALTIADKAYVLETGKVVLSG
                     TGKELLVSDQVRKAYLGG"
     gene            4728..5387
                     /locus_tag="VBC74_001765"
     CDS             4728..5387
                     /locus_tag="VBC74_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001934124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEO6942693.1"
                     /translation="MPVKDFMTKKLVYVSPDTTVAEAADLLREHHLRRLPVVENDQLV
                     GLVTEGTMAEAQPSKATSLSIYEMNYLLNKTKIRDIMIKDIVTVSQYASLEDAIYLMM
                     SRKIGVLPVVDNGQLYGIVTDRDVFKAFLEIAGYGQEGYRLVILADEGIGVLSKVLNR
                     LSSANLSVKRLVIIERKAGKKAVEIQLEGYADKDVLKQELVFDDVIVETLEKTMKKPL
                     S"
BASE COUNT         1677 a          798 c         1167 g         1751 t
ORIGIN      
        1 ttagaaagaa ggagttcaga tgtccttctt ttttatttag tttctataat tttcaaaaaa
       61 acaatttaaa aactattgac aatattctga taattctgta ttattttagt taccattttc
      121 aaaaaaggag aaaaatatgg aaaagagact tagtttagga gcgcttgttt tagctagtac
      181 agttctttta gcagcatgtg gaaatgtcgg aggaggagca tcttcgacag gaacaaaaat
      241 agggaaagat attaaggtag ggtataactg ggaattatca ggaaacgttt cttcatatgg
      301 aaattcaatg aagaatggag ctgacttagc agtaaaagaa attaatgctg ctggaggggt
      361 tggcggcaag aagttaaaag tcctatcaca ggataataaa tcagaaaatg cagaggcagc
      421 aacggttgcc acaaacttgg ttaccaaagg agctaatgtc attatcggac cagcaacatc
      481 gggtgcagct gcatcttcaa ctccaaaagt aaatgcagca gcagttccaa tgattgcacc
      541 tgctgcgaca caagacaatt tagtctatgg ttctgatgga aaaaccttaa atcagtattt
      601 cttccgagct acttttgtcg ataattatca aggaaagcta ttgtctcagt atgctacaga
      661 caaccttaaa gctaaaaaag ttgttctatt ttatgataat tcatcagatt actcaaaggg
      721 ggtagcaaaa tcatttaagg aaagttatag tggaaaaatt gttgatagta tgacattctc
      781 cgctggtgat actgatttcc aagcgtcatt gactaagttg aaagggaaag aatatgatgc
      841 tattgtgatg ccaggttact ataccgagac aggattaata gttaagcaag cgcgtgattt
      901 aggtatctct aaaccggttc ttgggcctga tggttttgat agtccgaaat ttgtgcaatc
      961 ggcaacacct gtaggagctt caaacgttta ttatttgaca ggtttcacta cacaaggatc
     1021 aaccaaagct aaagctttcc atgatcatta cgttaaggca tatggtgaag aaccatccat
     1081 gttctcggct ttatcatacg acgctgtgta tatggcagct aaatctgcta aaggagctaa
     1141 aacttcaatt gatattaaaa aagctttagc taagctgaaa gattttaaag gtgtgacagg
     1201 gaaaatgtct attgataaaa atcacaatgt tgttaaatca gcttacgttg tcaaattaga
     1261 ggatggaaaa acaagtagcg ttaacattat ttcagcaaaa taagtattct ctagaaaaat
     1321 gggagtttaa ttttcagctc ccctttttta aaaagtaatt tttcagaata attcgacaat
     1381 aatatataat agaaagtatg gttataatat gcttcaacag ctcgttaatg gacttatctt
     1441 aggatccata tatgctttac ttgccttagg atatactatg gtatatggca ttattaagct
     1501 gattaatttt gctcatgggg acatctatat gatgggcgct ttcatgggct attatttgat
     1561 taatcatctt catcttaatt ttttcctagc tttattaatt gcaatgctgg ggtctgcttt
     1621 cctaggtgtt gtaattgaat atttagctta tcgtccactg agaaaatcaa ctagaattgc
     1681 cgcgttaatt acagctattg gtgtttcttt tcttttggag tacgggatgg tataccttgt
     1741 tggagcggat actagggcat ttccgcaagc cattcatact gttaaatata atttaggacc
     1801 gataactata accaatgttc agctcatcat tttaggtatt gcgctattac taatgcttac
     1861 tttacagttc atcgttcaaa aaactaaaat ggggaaagct atgagagctt tatcagtgga
     1921 tagcgatgcc gcacaattga tgggaattaa tgttaaccgt accattagtt ttacatttgc
     1981 gctggggtct gctttagctg gagcaggtgg agtactgatt gggctttatt ataattctgt
     2041 tcaacctctt atgggggtaa ctccagggtt gaaagcattt gttgcagctg tcttaggtgg
     2101 tattggtatc attccgggag cggctatcgg tggttttgtt attggcattt tagaaacttt
     2161 agcgactgca cttggtgtgt cagattttcg agatggtatc gtttatgcta ttttaattct
     2221 cattttcttg atccgtccag caggaattct tggaaaaaat attaaggaga aggtctaggc
     2281 atgaaagaaa atatgaaatc aattttatct tggttagcta tagtagtgtt aggttatctc
     2341 ttgattagcg tgctaatatc tatgggtata tttaacctct atcatattca aattattgaa
     2401 acaattggga ttaatgttat tttagcagtt ggtttaaacc taattgtagg ttgttcggga
     2461 cagttttcac taggacatgc aggtttcatg gctattggcg cttatgcagt tgctattatc
     2521 ggcgttaaaa tgccaactta tgtaggtttt ttgatagcta tacttgttgg aactcttgtt
     2581 gcaggaggta ttgccttagg tgtaggaatt cctactttga gactaaaagg tgattattta
     2641 gctatagcta ctttaggtgt tgctgagatt atccgtattt tgttggttaa tggaggtgat
     2701 attacgaatg gtgctgcggg tatcatgggg attccgcctt ttacaacctg gtctttagtt
     2761 tatggggtgg cagttgtgag ccttatcctt gcaatgaatt ttttgcgtag tcccttaggg
     2821 cgtaatacaa ttgctatacg agaagatgaa attgcagcag agtctatggg tgtggataca
     2881 acaaaagtga aagtaattgt ttttgttttt ggagctattt tagcttctat cgctggtagt
     2941 ttacaggctg gctacgttgg aactgttatg ccaaaagact tttcatttat gatgtctgtc
     3001 aatgttttaa ttattgttgt tttaggtggt ttaggttcaa tgacagggac agttttagca
     3061 gcaattttat taggattatt gaatatgtta ctacaagact acgcttctgt tcgaatgatt
     3121 atctatgctt tagccctgat tttaattatg atttttagac ctagcggttt attaggaacg
     3181 aaagagttaa ctctatctca tctatttcgt aagacgagta agggaggcaa ttagatggca
     3241 cttcttgaag taaaaaattt aagtaaacat ttcggtggcc taactgctgt aggtgatgtt
     3301 tcgatgaaat tacataaagg tgaattaatt ggcttaatcg gccccaacgg tgcgggtaag
     3361 acaacgcttt ttaatctctt aacaggagta taccttccta gtaaagggac aatatcactt
     3421 gatggtaaga tattaaatgg gaggaaacca gctaaaatag cttctttagg tttaggtaga
     3481 acttttcaaa atatccgact ttttaaaaat atgactgttt tagacaatgt actagtagga
     3541 ctcagtaatc atcacttatc acatcctatt gctagttttt tacgattacc caaatactat
     3601 catagtgaaa aagccttaag gaaaaaggca ctggagctat tagagatttt tggtttgaaa
     3661 gcatatcaag atgctcttgc taagaacctc ccttatggaa aacaacggcg tttagaaatt
     3721 gttcgtgctt tagcaacaga accaaaaata ttgtttttgg atgagcccgc ggcaggaatg
     3781 aatcctcaag aaactgcaga attgacgcag ttaattagtc aaataaagag tgattttgat
     3841 attactatta tgctgattga acatgatatg aacttggtta tgcaagtaac agagagaatt
     3901 tatgtattgg aatatggtcg tctgattgct cacgggacac ctgaagaaat taaaaataat
     3961 aaacgtgtta tagaagcata cttggggggg gagctataat gaccatgttg aaagtagaaa
     4021 atctatctat tcactatggt gttattcaag cggtgaatga tgtctcgttt gaagttaacc
     4081 aaggtgaggt tgtaacctta attggagcca atggtgcagg aaagacatca attttgagga
     4141 caatttctgg tttagtaaga ccaagtcagg gttctatttc ttttatgggg aaacctatac
     4201 ataagttagc agctagaaaa attgttggca atggtttagc tcaggttcca gaagggcgcc
     4261 acgttttctc aagtttgtct gttatggaaa atttagaaat gggagccttt cttcaaaagg
     4321 atcgtgaaca aaatcaaaaa atgttaaaaa aagtgtttga tcgttttcct cgcttagaag
     4381 aacgtaaaaa ccaagatgca gcaaccttat caggtggtga gcagcaaatg ctcgctatgg
     4441 ggcgtgcatt gatgagtcgt cctaaattat tacttttaga tgaacagtca atgggcctag
     4501 ctccaatttt tattcaagaa atttttaata tcattgaaga catcaaaaaa caagggacta
     4561 cagttctttt agttgaacaa aatgcaaata aagctctgac gattgctgat aaagcatatg
     4621 ttctagagac tgggaaggtt gttttatcag ggacaggtaa ggaattacta gtttcagatc
     4681 aagttcgtaa agcttactta ggtggataat atttagagga gtcaggtatg ccagtgaaag
     4741 attttatgac aaaaaaatta gtttatgtct ctccagatac tacagttgca gaagctgctg
     4801 atttattgag agaacaccac ttaaggcgtt tgccagttgt tgaaaatgat cagctagtgg
     4861 ggttagtgac agaagggaca atggcagaag cacaaccttc taaagcaacc agtctttcta
     4921 tatatgagat gaactatctt cttaataaga caaaaattcg agatattatg attaaagata
     4981 ttgtgactgt ttcacagtat gctagccttg aggacgctat ttatttgatg atgtctcgaa
     5041 agatcggtgt tttaccagta gttgataatg gacaacttta tgggattgtc acggataggg
     5101 atgtttttaa agccttcctt gaaattgcgg ggtatggtca agaaggttat cgcttggtta
     5161 tattagctga tgaggggata ggggtactat caaaagtcct aaatcgtctt tctagtgcaa
     5221 atttaagtgt taaacgttta gtcattattg aacgaaaagc aggtaagaaa gcggtcgaga
     5281 tacaactaga aggatacgct gacaaggatg ttttaaaaca agaactagta tttgatgatg
     5341 ttattgtgga aactttagaa aagaccatga aaaaaccact ttcttaaaaa tat
//
LOCUS       DATROD010000114         5369 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.116, whole genome shotgun sequence.
ACCESSION   DATROD010000114 DATROD010000000
VERSION     DATROD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.116"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..327)
                     /locus_tag="VBC74_001766"
     CDS             complement(<1..327)
                     /locus_tag="VBC74_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942694.1"
                     /translation="MELIFRIFEMKKTLYITNKLFFANWFVPVIGAVLIQVIGIIVSR
                     RLVVKLLPDDYKFNLYIALLLFIPILASILLFLIYFQKRDFTSLGFTKDNNVLTTISG
                     FITGSLL"
     gene            complement(390..1151)
                     /gene="recO"
                     /locus_tag="VBC74_001767"
     CDS             complement(390..1151)
                     /gene="recO"
                     /locus_tag="VBC74_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEO6942695.1"
                     /translation="MRVSQTYGLVLYNRNYREDDKLVKIFTETEGKRMFFVKHASKSK
                     FNAVLQPLTIAHFILKINDNGLSYIDDYKEVLAFQETNSDLFKLSYASYITSLADVAI
                     SDNVADAQLFIFLKKTLELIEDGLDYEILTNIFEVQLLERFGVALNFHDCVFCHRAGL
                     PFDFSHKYSGLLCPNHYYKDERRNHLDPNMLYLINRFQSIQFDDLQTISVKPEMKLKI
                     RQFLDMIYDEYVGIHLKSKKFIDDLSSWGSIMKSD"
     gene            complement(1141..2316)
                     /locus_tag="VBC74_001768"
     CDS             complement(1141..2316)
                     /locus_tag="VBC74_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEO6942696.1"
                     /translation="MTLEKRFNKYLDRIEVSLIRQFDQSISDIPGMVKLTLGEPDFTT
                     PDHVKEAAKSAIDANQSYYTGMSGLLALRQAAADFAKDKYNLTYNPDSEILVTIGATE
                     ALSASLIAILEAGDVVLLPAPAYPGYEPIVNLVGADIVEIDTRENDFRLTPEMLETAI
                     IQQGEKLKAVLLNYPTNPTGITYSRQEIAALAEVLKKYDIFVISDEVYSELTYTGQQH
                     VSIAEYLPNQTILINGLSKSHAMTGWRVGLVYAPEAFTAQIIKSHQYMVTAASTISQF
                     AGVEALSVGKNDTLPMRQGYIKRRDYIIDKMSKLGFKIIKPSGAFYIFAKIPDSYPQD
                     SFKFCQDFAYQQAVAIIPGVAFGKYGEGYIRLSYAASMEVIETAMARLKVFMESYEG"
     gene            complement(2424..3392)
                     /locus_tag="VBC74_001769"
     CDS             complement(2424..3392)
                     /locus_tag="VBC74_001769"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEO6942697.1"
                     /translation="MSYSNLKLFALSSNKELAKKVSQTIGIPLGQSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAESVSVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRQ
                     GLVGDDVVVVSPDHGGVTRARKLAQCLKTPIAIIDKRRSVTKMNTSEVMNIIGNIKGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQNSAIEKLIVL
                     DTIYLPEERLIDKIEQISIAELIGEAIIRIHEKRPLSPLFEMNKLK"
     gene            complement(3516..4841)
                     /locus_tag="VBC74_001770"
     CDS             complement(3516..4841)
                     /locus_tag="VBC74_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEO6942698.1"
                     /translation="MKKRILSAVLVSGVTLGTAAVTVNADDFDSKIAATDSVINTLSG
                     QQAAAQNQVTAIKGQVGALESQQSELEAQNAQLEAVSQQLGQEIQTLSNKIVARNESL
                     KKQVRSAQKGNLTNYINTILNSKSVSDAVNRVVAIREVVSANEKMLAQQEADKAALEA
                     KQIENQNAINTVAANKQAIENNKAALATQRAQLEAAQLELSAQLTTVQNEKASLIQAK
                     AQAEEAARKAAEAQAVAEAKAQAESVAKAQAAAQVESATAPTETVQTQPRTEIKPSNL
                     TATSSATTVAKTTATATNEPKVTQPSVVTKAVEAPKAVVSSTPRAVSKPVVRSYDSSN
                     TYPMGQCTWGAKSMASWVGNYWGNANQWGASARAAGYSVGTTPRVGAVAVWPYDGGGY
                     GHVAVVTSVANNSSIQVMESNYAGNMSIGNYRGSFNPSASGSVYYIYPN"
     gene            complement(5077..5164)
                     /locus_tag="VBC74_001771"
     tRNA            complement(5077..5164)
                     /locus_tag="VBC74_001771"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5128..5130),aa:Ser,seq:gct)
     gene            complement(5178..5251)
                     /locus_tag="VBC74_001772"
     tRNA            complement(5178..5251)
                     /locus_tag="VBC74_001772"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5215..5217),aa:Ile,seq:gat)
     gene            complement(5286..5356)
                     /locus_tag="VBC74_001773"
     tRNA            complement(5286..5356)
                     /locus_tag="VBC74_001773"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5322..5324),aa:Gly,seq:tcc)
BASE COUNT         1775 a         1060 c          844 g         1690 t
ORIGIN      
        1 cagtaaacta ccagtaataa atcctgagat cgttgttaaa acattattat ctttagtaaa
       61 tcccaaggaa gtaaaatccc ttttttgaaa atagattaaa aatagtaaaa tacttgccaa
      121 aataggaata aatagtaaaa gcgcaatgta taaattgaat ttataatcat caggtagcaa
      181 ttttactaca aggcgtctac ttacaatgat gccgataact tgaattagaa ctgcaccaat
      241 aactggcaca aaccagttag caaaaaagag tttattggta atgtataatg tttttttcat
      301 ctcaaatatc ctaaaaatta attccatatt agtttatcct aatattaaaa aaaagaacat
      361 gacactaatg tcatgttcct ctttacaact taatctgatt tcataatact tccccaacta
      421 gacaaatcat caataaattt tttactttta agatgaatcc ctacatattc atcgtaaatc
      481 atgtccaaaa attgacgaat tttaagtttc atctcaggtt tcacagaaat tgtttgtaaa
      541 tcatcaaatt gaattgactg aaaacgattg attaagtaca gcatattagg atctaggtgg
      601 tttcttctct cgtctttata ataatggttt ggacataata atccagaata tttgtgtgaa
      661 aaatcaaaag gtaatcctgc cctatgacaa aaaacacaat catgaaaatt taaagcaaca
      721 ccgaacctct ctagtagttg tacttcaaaa atattcgtta gaatctcata atctaaaccg
      781 tcctcaatca attctaacgt tttctttagg aaaataaata attgagcatc cgctacatta
      841 tcactaatag ccacatcagc caaagaagta atataactcg catatgacaa cttaaacaag
      901 tctgaattgg tttcttgaaa tgctaaaact tctttataat catcaatata agaaagacca
      961 ttatcattaa tttttaaaat aaaatgagca atagtcaaag gttggagcac agcattgaat
     1021 tttgactttg aagcatgctt tacaaaaaac attcttttcc cctcagtctc cgtaaagatt
     1081 ttaactaatt tatcgtcttc acgataatta cgattataca aaacgagacc gtatgtttga
     1141 ctaaccctca tatgattcca taaaaacttt caatctagcc attgctgttt caataacttc
     1201 catactagca gcataagaaa gacgtatata cccttcacca tatttaccaa aagccacccc
     1261 tggaataata gctacagctt gttgataagc aaaatcctga caaaacttga aagaatcctg
     1321 tggataacta tcaggtattt tagcaaagat ataaaaagcg ccgctcggtt taattatttt
     1381 gaaaccaagt ttagacattt tatcaatgat ataatctcta cgtttaatat atccttgacg
     1441 cataggtaac gtatcatttt tacccacaga taaggcttct accccagcaa attgcgaaat
     1501 agtactagca gctgtcacca tgtattgatg acttttaatg atttgagcag taaatgcttc
     1561 aggagcatac accaatccaa ctctccaacc agtcatagca tgtgattttg ataagccatt
     1621 aattaagata gtttggttag gtaaatattc tgcaattgaa acatgttgct gaccagtgta
     1681 agttaactca gagtaaactt catcactaat aacaaaaata tcatattttt taaggacttc
     1741 agctaatgca gcaatttctt gccttgaata ggtaatacct gtaggattag taggataatt
     1801 aagtaaaaca gcctttaatt tttcaccttg ttggataata gccgtttcta acatttcagg
     1861 ggttagtcta aagtcatttt ctctagtatc aatttctacg atatcagctc ctacaaggtt
     1921 aactattggc tcatagcctg gataagcggg ggcaggtaat agaacaacat caccagcttc
     1981 taaaatagct ataagagatg cagacagagc ttctgtggca cctatcgtaa ccaagatctc
     2041 actatcaggg ttataagtaa gattatattt atcctttgca aaatctgcag cagcttgcct
     2101 tagagctaat aaaccgctca taccagtata atacgattga ttcgcatcaa tagctgattt
     2161 agctgcttct ttaacatgat caggagtggt aaaatctggt tctccaagag ttaattttac
     2221 cattccaggt atatctgaaa tagattgatc aaactgacga attaaggaga cctctattct
     2281 atctaagtac ttattaaatc gtttctctaa tgtcatcata acctcctgat aagaaataat
     2341 atatttaatt atacaagaaa aataatttgt gtccagtttt ccgtcattaa atgataaaaa
     2401 acctgttaat aacaggtttt ttatcatttt agtttattca tttcaaataa tggtgataaa
     2461 ggacgtttct cgtgaatacg gataatagct tcaccaatca attccgcaat tgaaatttgt
     2521 tcaatcttat caatcaaacg ttcttcaggg aggtaaatcg tatctaacac aattaatttt
     2581 tcaatagctg aattttgaat attatccaaa gccggccctg ataaaacagg gtgagtacat
     2641 gaagcataaa cagctgttgc ccctgcttca gctaaagcgt ctgcagcatg gcaaatagta
     2701 cctgcagtat caatcatatc atcaattaaa atacattttt tacctttgat attaccaata
     2761 atattcatta cttcagatgt attcatttta gtaacactac ggcgcttatc aatgatagca
     2821 atcggtgtct ttaaacactg tgccagctta cgtgcacgtg ttacgccacc atgatcagga
     2881 ctaacgacaa caacatcatc accaactaat ccctgacgat caaagtaatc tgcaataagg
     2941 ggagccccca taaggtgatc aacgggaata tcaaagaatc cctgaatttg agctgcatgt
     3001 aaatcaactg ttaaaagacg atcaacacca gcaacttcta gcatgttcgc aacgagtttc
     3061 gaagtaatag gttcacgaga acgtgccttg cgatcttgac gagcataccc atagtaaggc
     3121 attaccacac taacagactc cgcactagca cgctttaaag catcaaccat aattaaaatt
     3181 tccatcaaat tatcattaac tggtgagctt gttgattgta aaataaaaac atgatggcca
     3241 cgaatagact cttcaatatt aacttgaatc tcaccatcag aaaattggcg aactgtcgat
     3301 tgacctagcg gaattccaat cgtctgagac acttttttcg ctagttcttt gtttgatgac
     3361 agtgcaaaca attttaaatt agaataagac atgatttcct ccaaaacttt agtcctatac
     3421 cattttaacg ctttttacac aatttttcaa tccaaaataa accataccta attaataaac
     3481 aaaaaggaga ccacgaaaga tctccttagt attcattaat ttgggtaaat atagtaaaca
     3541 gagccagaag cactagggtt aaatgaacca cggtaattgc caatgctcat atttccagca
     3601 tagtttgatt ccataacttg gatagatgaa ttgttagcca ctgacgttac tactgcaacg
     3661 tgcccgtaac caccaccatc atatggccaa acagctactg caccaacacg aggggtagtt
     3721 cctacagaat aaccagctgc acgtgcacta gcaccccact gattagcatt tccccaatag
     3781 ttaccaaccc aagaagccat tgacttagct ccccaagtac attgtcccat tgggtatgtg
     3841 ttacttgaat cataacttct gacaacaggt tttgataccg ctctaggtgt tgaagaaaca
     3901 actgctttag gtgcttcaac tgctttagtt actacagaag gttgagtaac ttttggctca
     3961 ttagtagctg tagctgtagt tttagcaaca gtagttgccg aagaagtagc cgtaagatta
     4021 gaaggcttta tttcagttct cggttgtgtt tgaactgttt cagtaggagc tgtggcagac
     4081 tcaacttgtg ctgctgcctg tgcttttgct acagactcag cttgtgcttt cgcttcagct
     4141 actgcttgtg cttcagctgc cttacgcgca gcttcctcag cttgtgcttt tgcctgaatt
     4201 aaagatgctt tttcattttg gactgtagtc aattgagcag ataactctaa ttgagctgct
     4261 tctaattgtg ctctttgtgt tgctaaggct gctttattat tttcgatagc ttgcttgtta
     4321 gctgctacag tatttatagc attttgattt tcaatttgtt tagcttctaa ggctgctttg
     4381 tcagcctctt gttgtgctaa catcttttca ttagctgaaa caacctcacg aatagcaaca
     4441 acacgattaa cagcatcaga tactgatttc gaatttaaaa tagtattaat atagttagtc
     4501 aagttaccct tttgagcact acgaacttgc tttttcaaag attcgttacg tgcaacaatt
     4561 ttattagaaa gagtttgaat ctcttgacct aactgctgag atactgcctc aagttgagca
     4621 ttttgcgctt ctaattctga ttgttgagac tctaaagcac caacttgtcc tttaatagca
     4681 gtcacctggt tttgtgctgc tgcttgttgc cctgataaag tattgattac tgaatcagta
     4741 gcagcaattt tcgagtcaaa gtcatcagcg tttactgtta cggcagcagt tccaagagtt
     4801 actccactca caaggactgc tgataatatt ctttttttca tttttattaa actactcctt
     4861 tacgataaga cattaaatat tttaccaaaa aattcacgaa attatattac gtcattgtta
     4921 catttatatt tgaaatcaac tatttctaaa tgaaccataa tcaaatctag aaaacgataa
     4981 ccttcttcta ttcactctat caatataatt actccatagt gaaactaaaa gagaaataaa
     5041 aaaagagtat aattactctt aaaattataa tatttacgga gaataaggga ttcgaaccct
     5101 tgcgccagtt acccgaccta acgatttagc aaaccgtcct cttcagcctc ttgagtaatt
     5161 ctccaaatta atattaatgg gcacgagtgg actcgaacca ccgacctcac gcttatcagg
     5221 cgtgcgctct aaccacctga gctacgcgcc caagcaaatg cttggtttta cttttatgta
     5281 aagtaagcgg gtgacgagaa tcgaactcgc gacaacagct tggaaggctg tagttttacc
     5341 actaaactac acccgctaaa aacttatat
//
LOCUS       DATROD010000115         5326 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.117, whole genome shotgun sequence.
ACCESSION   DATROD010000115 DATROD010000000
VERSION     DATROD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.117"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            101..1333
                     /gene="arcA"
                     /locus_tag="VBC74_001774"
     CDS             101..1333
                     /gene="arcA"
                     /locus_tag="VBC74_001774"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEO6942699.1"
                     /translation="MTQTHPIHVFSEIGKLKKVMLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRNEGVEVLYLENLAAESLTNQEIREQFIDEYIGEANVRGRA
                     TKKAIRELLLNIKDNKELIEKTMAGIQKSELPEIPSSEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGNGVSLNHMFSETRNRETLYGKYIFTHHPEYGGKVPMVYDREETTRI
                     EGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHLD
                     TVFTMVDYDKFTIHPEIEGDLRVYSVTYENQDLHIEEEKGDLADLLAKNLGVEKVELI
                     RCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKINGSELV
                     RGRGGPRCMSMPFEREDL"
     gene            1339..1769
                     /locus_tag="VBC74_001775"
                     /pseudo
     CDS             1339..1769
                     /locus_tag="VBC74_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772116.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            1785..2798
                     /gene="argF"
                     /locus_tag="VBC74_001776"
     CDS             1785..2798
                     /gene="argF"
                     /locus_tag="VBC74_001776"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEO6942700.1"
                     /translation="MTQVFQGRSFLAEKDFSREEFEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTIKENFGKLEGITLVY
                     CGDGRNNVANSLLVAGTLMGVNVHIFSPKELFPAEEIVKLAEGYAKESGAHVLVTDNV
                     DEAVKGADVFYTDVWVSMGEEDKFKERVELLQPYQVNMELIKKANNDNLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            2861..4288
                     /gene="arcD"
                     /locus_tag="VBC74_001777"
     CDS             2861..4288
                     /gene="arcD"
                     /locus_tag="VBC74_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEO6942701.1"
                     /translation="MEKEKKLGLLPLTMLVIGSLIGGGIFDLMQNMSSRAGLVPMLIA
                     WVITAIGMGTFVLSFQNLSEKRPDLTAGIFSYAKEGFGNFMGFNSAWGYWLSAWLGNV
                     AYAALLFSSLGYFFKFFGNGNNIISIIGASIVIWVVHFLILRGVNTAAFINTVVTFAK
                     LVPVIIFLISALLAFKFNIFSLDIWGNGLHQSIFNQVNSTMKTAVWVFIGIEGAVVFS
                     GRAKKHSDIGKASILALFTMISLYVLISVLSLGIMSRPELANLKTPAMAYVLEKAVGH
                     WGAILVNLGVIISVFGAILAWTLFAAELPYQAAKEGAFPKFFAKENKNKAPINSLLVT
                     NLCVQAFLITFLFTQSAYRFGFALASSAILIPYAFTALYQLQFTLREDKSTPGHQKNL
                     IIGILATIYAIYLIYAGGFDYLLLTMIAYTLGMILYIKMRKDDKLPIFVRYEKISAIV
                     ILALCLLCIIEIMTGQIDIAAILAG"
     gene            4309..5265
                     /gene="arcC"
                     /locus_tag="VBC74_001778"
     CDS             4309..5265
                     /gene="arcC"
                     /locus_tag="VBC74_001778"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEO6942702.1"
                     /translation="MVKEHQKIVVALGGNAILSTDASAKAQQEALINTSKSLVKLIKE
                     GHDVIVTHGNGPQVGNLLLQQADSDSEKNPAMPLDTCVAMTEGSIGFWLQNALNNELQ
                     EQGIDKEVATVVTQVIVDEKDQAFTNPTKPIGPFLSEEDAKKQAQETGSKFKEDAGRG
                     WRKVVPSPKPVGIKEASVIRRLVDSGVVVISAGGGGVPVIEDANTKALKGVEAVIDKD
                     FASQTLSELVDADLFIVLTGVDNVFVNFNKPNQEKLEEVTVSQMKQYITENQFAPGSM
                     LPKVEAAIAFVENKPEARAIITSLENIDNVLAENAGTQIVVN"
BASE COUNT         1734 a          924 c         1014 g         1654 t
ORIGIN      
        1 aacttcccta ttttatattc aaaagaaagt agaatgtaag tgtagatata gagataaatc
       61 ctgtttcaat gcaaatctct aactaagaaa ggagtaaaat atgactcaaa cacatccaat
      121 tcatgttttc tcagaaattg gaaaacttaa aaaagttatg ctacaccgtc caggtaaaga
      181 aatagaaaat ctgatgcctg attatttaga gagactcttg tttgatgata tcccattttt
      241 ggaagatgca caaaaagaac acgatgcgtt tgcacaagca cttcgcaacg aaggagtcga
      301 agtactttac cttgaaaatc ttgctgccga gtcgctaaca aaccaagaaa ttcgtgaaca
      361 gtttatcgat gagtacattg gcgaagcaaa tgttcgcggt cgtgctacta aaaaagctat
      421 tcgagaatta ttactcaata tcaaagataa taaagaactt atcgaaaaaa ctatggcagg
      481 tatccaaaag tctgagctac ctgaaatccc atcttctgaa aaaggtctaa ctgatcttgt
      541 cgaatctaat tatccatttg ccatagatcc gatgccaaat ctttacttta cacgtgatcc
      601 atttgcaact attgggaatg gagtttcatt aaaccatatg ttctctgaaa cacgtaatcg
      661 cgaaacttta tacggtaaat atatctttac acatcaccca gagtacggtg gaaaagtccc
      721 tatggtctat gatcgtgaag aaacaactcg tattgaaggt ggagatgagc tcgtcctttc
      781 taaagatgtc cttgccgtgg gtatctctca acgtactgat gctgcctcta ttgaaaaact
      841 cttagtcaat atttttaaac aaaatttagg ttttaaaaag gttttagcat ttgaatttgc
      901 aaataatcgt aaatttatgc accttgacac agtctttaca atggttgact atgacaagtt
      961 tacaattcac ccagaaattg aaggcgatct tcgcgtttac tctgtaactt acgaaaacca
     1021 agatcttcat attgaagaag aaaaaggtga tttggcagat cttctagcga aaaacctagg
     1081 agttgagaaa gtggaattga ttcgttgcgg gggtgacaac ttagtcgctg ctggacgtga
     1141 acaatggaat gatggttcaa atacattaac tatagcacca ggtgtcgttg tcgtttacaa
     1201 cagaaataca atcacaaatg caatcttaga gtccaaagga ttgaaattaa ttaagattaa
     1261 tggaagtgaa ttagttcgtg ggcgtggtgg accacgctgc atgtcaatgc catttgaacg
     1321 tgaagacctt taaaatttat gaagctatta gtcaatcact tgccttacca aagagctgca
     1381 agcctatata ttcgcttttc tgttttcgtt atcgaaagaa atataaaaat gaaagaagaa
     1441 ttcgatgata atgacgagca agatactatt tatgctgttc tttatgatgg aaacagccag
     1501 tttcaacagg acgctttctt cctgaaactc aaacagaagc tcgtttgact cgaattgcga
     1561 ctttaaaagg ttatcgtggt aacggctatg gtaccaaaat tattatagca ctagaaaatt
     1621 atgcaaaaga aaatggttat cactatctaa ctatacatgc tgaattgaca gcaaaagatt
     1681 tctaccagac acttgggtat caagctactg ggaatattta tatggaagac ggggaagcct
     1741 gtcaaacact cgaaaaatat ttaatttaaa ggagacatta acatatgaca caagtatttc
     1801 aaggacgtag tttcttagca gaaaaagatt tttctcgtga ggaatttgaa tatcttattg
     1861 atttttcagc tcatttaaaa gaccttaaaa aacgtggtgt tcctcatcat tatcttgaag
     1921 gtaaaaatat tgctctctta tttgaaaaaa catctactcg tactcgcgca gcctttacaa
     1981 ctgcagcaat tgacctaggc gctcatccgg aataccttgg tgcaaatgat attcaacttg
     2041 gtaaaaaaga atcaacagaa gatactgcta aggttttagg acgtatgttt gatggtattg
     2101 aattccgtgg ttttagccaa agaatggttg aagagcttgc tgaattttct ggagtacctg
     2161 tctggaatgg tttaacagat gaatggcatc caacacaaat gctagctgac taccttacta
     2221 tcaaagaaaa cttcggtaaa cttgaaggta ttactcttgt ttactgtggt gacggacgta
     2281 acaatgttgc caactcgctt ttagtggctg ggactttgat gggggtcaat gtacacatct
     2341 tttctccaaa agaacttttc cctgctgaag agattgttaa attggctgaa ggatatgcca
     2401 aagaatctgg ggctcacgtt ctcgttactg ataatgtaga cgaagctgta aagggagcag
     2461 acgtctttta cactgatgtc tgggtatcga tgggagaaga agataagttc aaagaacgcg
     2521 ttgaacttct tcaaccatat caagtaaaca tggaactgat taaaaaagct aataatgata
     2581 atcttatctt cttacactgc ttacctgcat tccatgatac aaataccgtt tatggcaaag
     2641 acgtcgctga aaaatttggg gtcaaggaaa tggaagttac tgatgaagtc ttccgtagca
     2701 aatatgctcg tcatttcgac caagctgaaa atcgtatgca cactattaaa gctgtaatgg
     2761 ctgcaaccct tggaaatctt ttcattccaa aagtttaaat taaacgacta tagggagttg
     2821 acatatagtc aattccctat tttaggaaat gaggtataaa atggaaaaag aaaaaaaatt
     2881 aggtctttta ccactaacaa tgcttgtcat tggctctctt atcggtggcg gaatctttga
     2941 tttaatgcaa aatatgagtt ccagagccgg tttggtacca atgcttattg cttgggtaat
     3001 tactgctatc gggatgggaa ctttcgtttt aagttttcaa aatttatctg aaaaaaggcc
     3061 ggatctaaca gctggaatct ttagttacgc taaagagggg tttggaaact ttatgggatt
     3121 taactctgca tggggttatt ggttatcagc ttggcttgga aatgttgcct acgctgcact
     3181 cttattcagt tcactcggtt atttctttaa attctttggt aatggaaata atatcatctc
     3241 aattattgga gcaagtatag ttatttgggt tgtccatttc ttaattttaa gaggtgttaa
     3301 tacagctgca tttattaata ccgtagttac ctttgcaaaa ttagtacctg ttattatttt
     3361 cttaatttca gcgttattag ctttcaaatt taacattttt agtcttgata tctggggaaa
     3421 tggattacat caatcaattt tcaaccaagt caattcaact atgaaaaccg ctgtttgggt
     3481 atttattggt attgagggcg ccgttgtctt ctcaggtcgt gctaaaaaac actctgatat
     3541 tggtaaagca agtatcctag cattattcac tatgatttca ctttatgtat tgatttctgt
     3601 tttatcactt ggtatcatgt cacgtccaga acttgcaaac ttaaaaacac cagctatggc
     3661 ttacgttcta gaaaaagctg ttggtcactg gggtgctatc ttagttaacc ttggtgttat
     3721 catttcagta tttggcgcta ttcttgcttg gactttattt gcagcagaat taccatatca
     3781 agctgctaaa gaaggtgctt ttcctaaatt ttttgcaaaa gaaaataaaa acaaagctcc
     3841 aatcaactca ctcttagtca ctaatctttg tgtacaagca ttcttaatca cgttcttatt
     3901 cacacaaagt gcttatcgtt ttggtttcgc attagcatca tctgctatct taattcctta
     3961 tgcttttaca gcactatatc aattacaatt cacactccgt gaggataagt caactccagg
     4021 acatcaaaag aatttaatta tcggtatcct cgctacaatc tatgctattt accttatcta
     4081 cgctggtggt tttgattact tacttttgac aatgattgct tatactctag gtatgattct
     4141 ctatattaaa atgagaaaag atgacaagct tcctatcttt gtgagatatg aaaaaattag
     4201 tgccattgtc attctagcgc tttgcttact atgtattatt gaaattatga ctggacaaat
     4261 tgacattgct gcaatcctag caggttagtc attagaaaga gggataaatt ggtaaaagaa
     4321 catcaaaaaa tcgtagttgc cttaggtggt aacgctattc tatcaacaga tgcttcagca
     4381 aaagcacaac aagaagcatt aatcaacaca tcaaaatcac tggtcaaact gattaaagaa
     4441 ggccatgatg tcattgttac tcatggaaat ggtccccaag ttggtaactt attgctacag
     4501 caagcagatt cagattctga aaagaatcca gcaatgcctt tggatacttg tgttgccatg
     4561 acagaaggaa gtattggttt ttggttacaa aatgctctta acaatgaatt acaagaacaa
     4621 ggcattgaca aagaggttgc aacagtggta actcaagtta ttgtagatga aaaagatcaa
     4681 gctttcacta atccaacaaa acctattggc ccattcttat ctgaagaaga tgccaagaaa
     4741 caagcccaag aaacaggttc aaaatttaaa gaagatgctg gtcgtggatg gcgtaaagtc
     4801 gttccttcac caaaaccggt tggtatcaaa gaagcttctg ttattagacg gttagtggac
     4861 tctggtgtgg ttgttattag cgcaggtggc ggaggtgtac cagttattga agatgctaat
     4921 acaaaagcat taaaaggggt tgaagctgtt attgacaaag actttgccag tcaaacttta
     4981 tcagaacttg ttgatgccga cttattcatc gtcttaactg gcgttgacaa cgtttttgtt
     5041 aattttaata aacctaatca agaaaaatta gaggaagtta ctgtttcaca aatgaaacaa
     5101 tatatcactg aaaatcaatt tgcccctggc agtatgctac caaaggtaga agctgcgatt
     5161 gcttttgttg aaaataaacc agaagcgcgt gctatcatta cttcacttga aaatattgat
     5221 aatgtcttag cagaaaatgc agggacacaa attgttgtaa attaaacaat gtagattacc
     5281 attacataca ctccttatta ataacaaagc aatgtcactc cttagg
//
LOCUS       DATROD010000116         5301 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.118, whole genome shotgun sequence.
ACCESSION   DATROD010000116 DATROD010000000
VERSION     DATROD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.118"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            21..1268
                     /locus_tag="VBC74_001779"
     CDS             21..1268
                     /locus_tag="VBC74_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEO6942703.1"
                     /translation="MEKNTWKKLLVSTAALSVVAGGAIAATHSNSVDAASKTTIKLWV
                     PTDSKASYKAIVKKFEKENKGVTVKMIESNDSKAQENVKKDPSKAADVFSLPHDQLGQ
                     LVESGVIQEIPEQYSKEIAKNDTKQSLTGAQYKGKTYAFPFGIESQVLYYNKTKLTAD
                     DVKSYETITSKGKFGQQLKAANSYVTGPLFLSVGDTLFGKSGEDAKGTNWGNEAGVSV
                     LKWIADQKKNDGFVNLTAENTMSKFGDGSVHAFESGPWDYDAAKKAVGEDKIGVAVYP
                     TMKIGDKEVQQKAFLGVKLYAVNQAPAGSNTKRISASYKLAAYLTNAESQKIQFEKRH
                     IVPANSSIQSSDSVQKDELAKAVIEMGSSDKYTTVMPKLSQMSTFWTESAAILSDTYS
                     GKIKSSDYLKRLKQFDKDIAKTK"
     gene            1366..2736
                     /locus_tag="VBC74_001780"
     CDS             1366..2736
                     /locus_tag="VBC74_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO6942704.1"
                     /translation="MNKKLISLDGMSLKEVFQKGDLATKLSFLIMGLANLKNKQIVKG
                     LLFLISEILFLITFVYQVIPAVKGLISLGTQEQGMTTKTVDGIKIQVATQGDNSMLML
                     IFGLASLIFCCVFAYIYWSNIKSAAHLLTLKEEGREIPSFKKDIKSLTDGRFHMTLMS
                     IPLIGVLLFTILPLVYMICLAFTNYDHNHLPPKSLFDWVGFANFGNIFSGRMASTFFP
                     VLSWTLIWAVFATVTNFFFGIILALLINTKGLKFKKMWRTIFVITMAVPQFISLLIMR
                     NLLSDAGPVNALLIKWGLISSAHPLPFLSDPVWAKFSIIFVNMWVGIPVTMLVATGII
                     MNLPAEQIEAAEIDGANKFQVFQSITFPQILLIMTPTLIQQFIGNINNFNVIYLLTQG
                     GPTNSTYYQAGSTDLLVTWLYNLTVTAADYNLASVVGILIFILSAVFSLLAYTRTNSY
                     KEGAAK"
     gene            2736..3572
                     /locus_tag="VBC74_001781"
     CDS             2736..3572
                     /locus_tag="VBC74_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEO6942705.1"
                     /translation="MNKKKRLNLTFVYILLIVLSIMWLFPIVWVVLTSFRGEGSAFVN
                     YFIPKTWTLDNYAKLFTQNTFPFGQWFLNTLFVATCTCILSTLITVAMAYSLSRIKFK
                     HRNGFLKLALVLNMFPGFMSMIAVYYILKALNLDQTLTALIFVYSAGAALTFYIAKGF
                     FDTIPYSLDESAMIDGATRLDIFLKITLPLSKPIIVYTALIAFMGPWMDFIFAKVILG
                     DATSKYTVAIGLFSMLQQDTINQWFMSFTAGSVIIAIPITILFMFMQKYYVEGITGGS
                     VK"
     gene            3803..5275
                     /locus_tag="VBC74_001782"
     CDS             3803..5275
                     /locus_tag="VBC74_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6942706.1"
                     /translation="MEKTKSFFGHPRGLSTLFFTEMWERFSYYGMRAILLYYMYYSVS
                     QGGLGMDKTVAASIMAVYGSLVYLSSVIGGFVSDRILGSRKTVLYGGILIMLGHIALA
                     TPFGQTALFISIALIILGTGLLKPNVSEMVGNLYGENDSRRDAGFSIFVFGINLGAFI
                     SPIVVGYLGQEVNFHLGFSLAAIGMFFGLLQYTLDGKKYLTEESLRPNDPLSPEEKSS
                     LYKKVGLILIGIVIVLILLHLMHMLTIEVIIDIFSIIAIAIPIIYFIKILSSKKISSV
                     ERSRVWAYIPLFIASILFWSIEEQGSVVLALFADEQTKLYLNFFGHHINFPSSYFQSM
                     NPLFIMLYVPFFAWLWAKWGSKQPSSPKKFAYGLFFAGASFLWMMLPGLLFGVNAKVS
                     PLWLTMSWAIVIVGEMLISPVGLSATSKLAPKAFQAQMMSIWFLSNAAAQAINAQIVK
                     LYTPDTQILYYGVVGGITVVFGFILLFYVPRIEKLMSGVK"
BASE COUNT         1615 a          982 c          921 g         1783 t
ORIGIN      
        1 tctctaggag gagaaccaaa atggaaaaaa atacttggaa aaaattactt gttagtactg
       61 ctgctctttc agtagttgca ggaggagcaa ttgctgctac tcactctaac tcagttgatg
      121 ctgcttcaaa aacaactatc aaactttggg tcccaacaga ttcaaaagcg tcttataaag
      181 caattgttaa aaaattcgag aaggaaaaca aaggcgttac tgtaaaaatg attgagtcta
      241 atgactccaa agctcaagaa aacgtaaaaa aagacccaag caaggcagcc gatgtattct
      301 cacttccaca tgaccaactt ggtcaattag tagaatctgg tgttatccaa gaaattccag
      361 agcaatactc aaaagaaatt gctaaaaacg acactaaaca atcacttact ggtgcacaat
      421 ataaagggaa aacttatgca ttcccatttg gtattgaatc tcaagttctt tattataata
      481 aaacaaagtt aactgctgac gacgttaaat catacgaaac aattacaagc aaagggaaat
      541 tcggtcaaca gcttaaagca gctaactcat atgtaacagg tcctcttttc ctttctgtag
      601 gcgacacttt atttggtaaa tctggtgaag atgctaaagg cactaactgg ggtaatgaag
      661 caggtgtttc tgtccttaaa tggattgcag atcaaaagaa aaatgatggt tttgtcaact
      721 tgacagctga aaatacaatg tctaaatttg gcgatggttc tgttcatgct tttgaaagtg
      781 gaccatggga ttacgacgct gctaaaaaag ctgtcggtga agataaaatc ggtgttgctg
      841 tttacccaac aatgaaaatc ggtgacaaag aagttcaaca aaaagcattc ttgggcgtta
      901 aactttatgc cgttaaccaa gcacctgctg gttcaaacac taaacgaatc tcagctagct
      961 acaaactcgc tgcatatcta actaatgctg aaagtcaaaa aattcaattc gaaaaacgtc
     1021 atatcgttcc tgctaactca tcaattcaat cttctgatag cgtccaaaaa gatgaacttg
     1081 caaaagcagt tatcgaaatg ggtagctcag ataaatatac aacggttatg cctaagttga
     1141 gtcaaatgtc aacattctgg acagaaagtg ctgctattct tagcgatact tacagtggta
     1201 aaatcaaatc tagcgattac cttaaacgtc taaaacaatt cgataaagac atcgctaaaa
     1261 caaaatagta cgtctagagt aatgagatga ggtgagtact cttcctttca attgagagtg
     1321 cttcccatct cttattttgt aaaataacaa ttaaaggaga ccattatgaa taaaaaactg
     1381 atctcacttg atggtatgtc cctaaaagag gtctttcaaa aaggtgattt agcaactaaa
     1441 ctatcattct taattatggg acttgctaac ctcaaaaata aacaaattgt caagggactt
     1501 ttatttttaa tcagtgaaat tctgttttta attacctttg tttaccaagt tatcccagct
     1561 gtaaaaggtc ttatttcctt agggacccaa gaacaaggta tgacaactaa aactgtggat
     1621 ggtattaaaa tccaagttgc gacacaaggt gataactcta tgttaatgct aatttttgga
     1681 ttagcttcgc ttatcttctg ttgcgtattt gcctatattt actggtcaaa cattaaaagc
     1741 gcagcacatt tattgacctt gaaagaagaa ggaagagaaa ttccatcctt caaaaaagat
     1801 attaaaagct tgacagatgg acgcttccat atgactttaa tgtctattcc tttaataggg
     1861 gtgcttctat ttacaatcct tccgttagtt tatatgattt gtttggcatt tactaactat
     1921 gatcataacc acttaccgcc aaaaagttta tttgactggg ttggttttgc taacttcggt
     1981 aatatcttta gcgggcgtat ggccagtact ttcttccctg ttctatcttg gacattaatt
     2041 tgggctgttt tcgctactgt aacgaatttc ttctttggta ttattcttgc cttactaatt
     2101 aatacaaaag gtttgaaatt caaaaagatg tggcgtacta tttttgttat taccatggct
     2161 gtccctcaat ttatctctct cttaatcatg agaaacctct taagtgacgc tggaccggtt
     2221 aacgccttgt taataaaatg gggacttatt tcttcagcac atccccttcc attcctttct
     2281 gatccggtct gggcgaaatt ctcaatcatc tttgttaata tgtgggttgg tattcctgta
     2341 acaatgcttg ttgctactgg tatcatcatg aatttacctg cagaacaaat tgaagctgct
     2401 gaaattgatg gggcgaataa gttccaagtc tttcaatcaa ttactttccc acaaattttg
     2461 ttgattatga ctcctacttt aattcaacaa tttatcggaa atattaacaa cttcaacgtt
     2521 atctacctac tcactcaagg tggaccaaca aactcaacat actaccaagc aggtagcacc
     2581 gatttacttg tcacttggct atacaatttg acagtaactg ctgcagatta taacttagca
     2641 tctgttgttg gtatcttaat ctttattctt tcagcagtat ttagtttact agcttacaca
     2701 agaacaaatt cttacaagga aggggcagct aaataatgaa taagaaaaaa cgtcttaacc
     2761 ttacttttgt ttatatttta ttgatagtac tatcaattat gtggctcttt cccattgttt
     2821 gggtcgttct cacaagcttc cgtggtgaag gaagtgcctt tgtcaactac tttattccta
     2881 aaacttggac attagacaac tacgctaaac tcttcaccca aaacacattc ccatttggtc
     2941 aatggttctt aaatacatta tttgtcgcta catgtacttg tatactatca actttaatca
     3001 ctgtagcaat ggcttattct ctcagcagaa tcaaattcaa acaccgtaat ggtttcctaa
     3061 agctagcttt agtcttgaat atgtttcctg ggttcatgtc aatgattgca gtttactaca
     3121 tcctaaaagc tctaaatcta gaccaaacat taaccgcttt aatatttgtc tattctgcag
     3181 gagcagcatt aaccttctac atcgcaaaag gtttctttga tactattcca tactctttgg
     3241 atgagtctgc tatgattgat ggggcgaccc gtttggatat tttccttaaa atcaccctac
     3301 cattgtcaaa acccattatc gtttatactg cattaatcgc ttttatgggc ccttggatgg
     3361 actttatctt tgcaaaagtt attttaggtg atgctacgag caagtacaca gtcgctattg
     3421 gtctcttttc aatgttgcaa caagatacca tcaaccagtg gttcatgtca tttacagcag
     3481 gatctgttat tattgcaatt ccaatcacaa tacttttcat gttcatgcaa aaatactatg
     3541 ttgaaggtat tacaggtgga tctgtaaaat aatcttcaga taatttaaag acacgacatt
     3601 tttatttcac tgtggtttct tttctaaaaa aggcttgggt tctattgaat tcaagccttt
     3661 ttctaagcta gatagttaaa aaacttggtt taccaagggt gttaacctct acaaaaaaat
     3721 aacttgatag tgctaagtaa tttgacagtt gcttctattt actatattat taatagtgtt
     3781 atctacaaaa ggagcgtttt tcatggaaaa aacaaaatca ttctttggac atcctcgtgg
     3841 tttgtccact cttttcttta ctgaaatgtg ggaaagattc tcatactatg gtatgcgtgc
     3901 tattttgctg tactatatgt actatagtgt ttctcaaggt ggccttggta tggacaagac
     3961 tgtcgctgca tcaatcatgg ctgtctatgg ttcactggtt tatctctcat cagtaattgg
     4021 tggttttgtc agcgaccgta ttctaggtag tcgtaaaact gttctgtatg gtggtattct
     4081 aatcatgcta ggtcatattg ctctagctac accatttggt caaacagctc tattcatttc
     4141 gattgctcta attatccttg gtactggact attaaaacca aatgtatcag agatggttgg
     4201 taatttatat ggagaaaatg attctcgtcg tgatgctggt tttagtatct ttgtttttgg
     4261 tattaacctt ggtgctttta tttcacccat tgtagtgggg tacctcggac aagaagtaaa
     4321 tttccatctt ggtttctcac ttgctgctat tggtatgttc tttggtctcc tccaatatac
     4381 cttagatgga aaaaaatatt tgactgaaga gagtctcaga ccaaacgatc ctttaagtcc
     4441 tgaagaaaag tcctctctat ataaaaaagt tgggcttatc cttattggta ttgttattgt
     4501 acttattcta cttcacttga tgcatatgct aacaattgaa gtaattatcg atatttttag
     4561 tattattgca atcgccatcc caattattta ttttatcaag attttaagta gtaaaaagat
     4621 ttcttctgtt gagcgttctc gagtgtgggc atatatccct ctctttatcg cctcaattct
     4681 attttggtca attgaagaac aaggttcagt tgtcttagcc ttatttgcag atgaacaaac
     4741 aaaactttac cttaacttct ttgggcatca tattaatttc ccatcaagtt atttccaaag
     4801 tatgaaccct ctcttcatta tgctttatgt accattcttt gcttggttat gggctaaatg
     4861 gggaagtaag caaccttcat cacctaaaaa atttgcgtat ggacttttct ttgctggagc
     4921 ttcattctta tggatgatgc taccaggttt actctttgga gttaacgcta aagtaagccc
     4981 tctttggtta acaatgagtt gggctattgt catcgttggg gaaatgctaa tctcaccagt
     5041 tggattatca gcaactagta agctcgcacc taaagcattc caagctcaaa tgatgagcat
     5101 ctggttctta agtaatgctg cagcacaagc tattaacgct caaatcgtta aattgtacac
     5161 acctgatact caaattcttt attatggtgt tgttggtggt ataacagttg tatttggatt
     5221 tatcctctta ttttatgttc cacgcattga aaaactaatg tctggagtta aataaagaca
     5281 tctaaaaaga actaggttac a
//
LOCUS       DATROD010000117         5245 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.119, whole genome shotgun sequence.
ACCESSION   DATROD010000117 DATROD010000000
VERSION     DATROD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.119"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(233..2497)
                     /gene="glgP"
                     /locus_tag="VBC74_001783"
     CDS             complement(233..2497)
                     /gene="glgP"
                     /locus_tag="VBC74_001783"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEO6942707.1"
                     /translation="MTRNFTTYVGQQGKVLSELTNEEIYVELLNFVKEEAAAKSKNSS
                     QRKVYYISAEFLIGKLLSNNLINLGIYKDVKKELELVGKSIAEIEDVEPEPSLGNGGL
                     GRLASCFIDSISSLGINGEGVGLNYHCGLFKQVFRNNQQEAEANYWIENNSWLVPTDI
                     SYDVPFRDFTLKSRLDRIDVLGYKKDTKNYLNLFDIDGLDYNLIEKGITFDKTEIKKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLLIDEAIERGSNLHDLAEYAYVQINDT
                     HPSMVIPELIRLLTEKHGFEFDEAVSVVRNMVGYTNHTILAEALEKWPLEYLNEVVPH
                     LVTIIKKLDQMIREEQTNPEVQIIDEAGRVHMAHMDIHFSTSVNGVAALHTEILKNSE
                     LKVFYDIYPDKFNNKTNGITFRRWLEFANQDLADYLKELIGDSYLTDATQLEKLLTYA
                     DSNEVHDKLAAVKFKNKLALKRYLKENKGIELDEYSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKRGHFPSRKLTVIFGGKAAPAYTIAQDIIHLILCLSELINNDPEVNKYLNV
                     HLVENYNVTVAEKLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGKENIYTFGKDSDTIINLYETSGYRSKDYYDKDKVIREAVDFIISDDIVSLGNAE
                     RLKRLHDELVGKDWFMTLIDLKEYIAVKEQVLADYEDYESWNKKVIHNIAKAGFFSSD
                     RTIEQYNQDIWHSN"
     gene            complement(2509..4005)
                     /gene="malQ"
                     /locus_tag="VBC74_001784"
     CDS             complement(2509..4005)
                     /gene="malQ"
                     /locus_tag="VBC74_001784"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEO6942708.1"
                     /translation="MKKRASGVLMHITSLPGDLGIGTFGREAYAFVDFLVETDQKFWQ
                     ILPLTTTSFGDSPYQSFSAVAGNTHLIDFDLLTLEGFISKDDYQNISFGQDPEVVDYA
                     GLFEKRRPVLEKAVKNFLQEERATRMLSDFLQEEKWVTDFAEFMAIKEHFGNKALQEW
                     DDKAIIRREEEALAGYRQKLSEVIKYHEVTQYFFYKQWFELKEYANDKGIQIIGDMPI
                     YVSADSVEVWTMPELFKLDCDKQPLAIAGVPADDFSDDGQLWGNPIYNWDYHKESDFD
                     WWIYRIQSGVKMYDYLRIDHFKGFSDYWEIRGDYQTANDGSWQPAPGPELFATIKEKL
                     GDLPIIAENLGYIDERAERLLAGTGFPGMKIMEFGFYDTTGNSIDIPHNYTENTIAYA
                     GTHDNEVINGWFENLTVEQKAYAENYMRRLPNEPITETVLRTLYATVSQTTITCMQDL
                     LDKPADSRMNMPNTVGGNWQWRMRKEDLTENRKAFLKEITTIYNRGNK"
     gene            complement(4126..5154)
                     /locus_tag="VBC74_001785"
     CDS             complement(4126..5154)
                     /locus_tag="VBC74_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEO6942709.1"
                     /translation="MVTIKDVAAKAGVNPSTVSRVLKDNESISSKTKERVKKAMEELG
                     YVPNVAAQMLASGLTQNIGVIFPPLVLPDRLSEPFFMEILSTLTNEAKEHGFTVSIAT
                     SMSSEELLEQVKLMHLQKRVDGFIVLYSDQKDPIREYLLKYKLPFVVVGAANGYENEI
                     TYIDNDNQLMGKSAIDYLYKKGHKSIQFVTDDLNSEVSEERYLGYFKGARKLGLNQKP
                     ALLFDRGNPQVLEEFINRVKEEETTALIVIGDTVSVRVMQFLSFYKLKVPDDISIMTF
                     NNSLFSHLIHPYLSTFDINVNNLGRTSVRRLIDIIKSPDKVFSETIIVPFTLEERESV
                     RDISKNNN"
BASE COUNT         1673 a          994 c          771 g         1807 t
ORIGIN      
        1 tcaaattata ttttttaatt ttagtttata tttcaaaaat taaaacaccc tataaccaag
       61 tatcacagag tgcatatttt tatattatat agaatataaa tacacatgca attaaatatt
      121 tagaataagt tctgctcagt tctattttta aagtctacgg acaaaaataa atacttatag
      181 ttttattaga caagaaaaaa gtaaggttga aaaccttact tttagagact ttttaatttg
      241 aatgccaaat atcttgatta tattgttcga ttgtacgatc tgatgagaag aaccctgctt
      301 tagcaatatt gtgtataact ttcttattcc atgattcata atcttcataa tcagcaagca
      361 cttgttcttt aacagcaatg tattctttca aatcaatcaa tgtcatgaac caatctttac
      421 ctactaactc atcatgcaat ctctttagtc gttctgcatt accgagtgac actatatcat
      481 cactaatgat aaaatcaaca gcttcacgaa ttactttgtc tttatcatag taatcttttg
      541 aacgataacc agatgtttca taaagattaa tgatagtatc agagtcttta ccaaatgtgt
      601 agatgttctc tttgccagct aattcagcta tttcaacatt tgccccatcc atagtaccaa
      661 gagtaagagc accattcaac ataaatttca tgttacctgt accagatgct tctttggaag
      721 ccaatgaaat ttgttccgaa atatctgttg cagggattaa cttttccgct actgtcacat
      781 tgtaattttc tactaaatgg acattaaggt atttattaac ttctggatca ttattaatta
      841 attctgataa gcagagaatt aagtgaatga tatcctgtgc aatagtatag gctggagcag
      901 ctttaccacc aaaaataact gttaatttac gtgatgggaa atgcccacgt ttaatttcta
      961 agtatttatg aataacatat aaagcattca tttgttggcg cttatattcg tggaatcgtt
     1021 tgatttgtgt atcaataatt gagtattcat ccaattcaat acctttattt tccttaaggt
     1081 aacgtttaag agccagtttg tttttaaatt taacagcagc taatttatca tgaacttcat
     1141 tgctatcagc ataagttaac aatttttcga gctgagtcgc atcagtaaga tagctatccc
     1201 caattagttc tttcaagtaa tctgctaaat cttggttagc aaactccaac cagcgacgga
     1261 atgtaatacc gtttgtctta ttgttaaact tatctgggta aatatcatag aaaaccttta
     1321 attcactatt tttaaggatt tctgtatgta atgctgctac accattaacg cttgttgaga
     1381 agtggatatc catatgcgcc atgtgaacac gtccagcttc atcaataatt tgaacctctg
     1441 gatttgtttg ctcttcacga atcatttgat ctaatttttt aatgattgtt actaggtggg
     1501 gtactacctc attaagatac tctaaaggcc atttttcaag agcttctgca aggatggtat
     1561 ggtttgtata gccaaccata ttgcgaacaa ctgacacagc ttcgtcaaat tcaaaaccat
     1621 gtttttctgt caataaacga atcaactcag ggatgaccat agatgggtga gtgtcattaa
     1681 tttgaacata agcatattca gctaaatcat gaaggtttga accacgctcg attgcttcat
     1741 caattaataa ttgagcagca tttgagacca tgaaatattg ctgatagata cgtaataatt
     1801 ctccattttt atctgaatca tctggataca agaacaaggt taaattcttc ttgatttctg
     1861 tcttatcaaa agtgatacct ttttcaataa ggttataatc aagtccatca atatcaaaaa
     1921 ggttcaaata atttttagta tctttcttat aacctaatac atcaattcta tctaggcgtg
     1981 attttaaagt aaagtcacgg aaaggaacat cataagaaat atctgttggt actaaccaag
     2041 aattattctc aatccagtag ttagcttctg cttcttgttg attgttgcga aatacttgtt
     2101 tgaaaagccc acagtgatag tttaaaccaa caccttcacc attaatacct aaagaagaaa
     2161 tcgagtcaat aaaacatgat gctaaacgcc ctaaaccacc attaccaagt gatggttctg
     2221 gctctacatc ttctatttca gcaattgatt tacctacaag ttctaactct tttttgacat
     2281 ctttataaat ccctaagtta atcaaattat ttgaaagtaa tttaccaatt agaaactcag
     2341 ctgaaatata gtatacctta cgttgagagc tattttttga tttagcggca gcttcttctt
     2401 ttacaaaatt taacaattca acgtaaatct cttcatttgt taattctgat aatacttttc
     2461 cttgttgtcc aacgtaagtt gtaaaatttc ttgtcatgat aatattcctt atttattccc
     2521 tctattataa atagttgtaa tctcttttaa gaatgctttg cgattttctg ttaagtcttc
     2581 ttttctcata cgccattgcc agttacctcc aacggtattt ggcatgttca tacggctatc
     2641 tgcaggttta tctagtagat cttgcataca tgtaatcgtt gtttgactaa cggttgcata
     2701 caaggtacgc agtacagtct cagtaatggg ctcatttggc aaacgacgca tgtaattttc
     2761 tgcataagct ttctgttcga ctgtaagatt ttcaaaccag ccattaataa cttcgttatc
     2821 atgagttcca gcatatgcaa ttgtattttc tgtgtagtta tgaggaatat caatactatt
     2881 tcctgttgta tcataaaaac cgaattccat gattttcata cctgggaacc ctgttccagc
     2941 cagtaatctc tctgctctct catcaatata gcctaaattc tcagcaataa taggtaaatc
     3001 acctaatttt tctttgatag ttgcaaataa ttctggaccg ggagctggtt gccatgagcc
     3061 atcattagct gtttgataat ctcctcgaat ttcccaataa tctgaaaaac ctttaaaatg
     3121 atcaatacgc aaatagtcat acatcttaac accagattga atacgataaa tccaccagtc
     3181 aaaatctgac tctttgtggt aatcccaatt gtaaatagga ttgccccaaa gctgaccatc
     3241 gtcagaaaaa tcatccgctg gtacaccagc aatagcaagt ggttgtttgt cgcaatctaa
     3301 tttaaacagt tcaggcattg tccaaacttc tacactatcg gcagaaacgt agattggcat
     3361 atcaccgata atttgaatcc ctttatcatt agcatattct tttaactcaa accattgttt
     3421 gtaaaagaaa tattgcgtta cttcatgata ttttatcact tcactaagct tttgacgata
     3481 tcctgctaag gcttcttctt cgcggcgtat aatagccttg tcatcccatt cttgaagcgc
     3541 cttattacca aaatgttctt tgatcgccat aaattcagca aaatcagtta cccatttttc
     3601 ttcttgcaag aaatcagata gcattctcgt agctctctct tcttgcaaga aatttttaac
     3661 tgctttttct aaaactggac ggcgtttttc aaacagacca gcataatcaa caacttctgg
     3721 atcctgtcca aaactaatat tttggtagtc atctttcgaa atgaaacctt caagcgttag
     3781 caaatcaaaa tcaattaaat gtgtattacc agctactgca gaaaaagact ggtaaggaga
     3841 gtccccgaag cttgttgttg ttaagggaag aatttgccaa aatttttgat cagtttcgac
     3901 caaaaaatca acaaaagcgt aggcttctcg tccaaatgtt ccaataccta aatcacctgg
     3961 tagagaagtg atgtgcatta agacaccact tgcacgtttt ttcatattga ttctcctttt
     4021 cgattactta ctttactcct ccatcttaac gcaagcgatt gcataagtca agcataattt
     4081 aaaaaaactt ctatcgaaaa ttgatagaag tctttatatc atagcttaat tattattttt
     4141 agaaatatct cggacacttt cacgctcttc taaagtaaaa ggcacaataa tagtctcact
     4201 aaagactttg tctggagatt taatgatgtc aatcagacga cgaacgcttg tccttcctag
     4261 attattaaca ttaatatcaa atgtgcttag gtaaggatga atcagatgtg agaagagaga
     4321 attattaaag gtcatgatag aaatgtcatc tggaactttt aatttataga aagaaagaaa
     4381 ttgcattact ctaacagaga cagtatcacc aataacaatt aaagcagttg tttcttcttc
     4441 tttgactcta tttataaatt cctctaaaac ttgcggattg cctctgtcaa ataaaagagc
     4501 tggcttttgg tttaaaccta actttctagc acctttaaaa taccctaaat agcgctcttc
     4561 tgatacttct gaatttaaat catctgtaac aaactggatg cttttatgtc cttttttata
     4621 gagataatca atggcagatt tccccatcag ctgattatca ttatcaatat aagtaatctc
     4681 attttcatag ccattagcag caccaacaac aacaaagggc agtttatatt ttaataaata
     4741 ctctctgata ggatcttttt gatccgaata gagaacaata aaaccatcca ctcgtttttg
     4801 tagatgcatt aattttactt gttctaacaa ctcctcagaa gacatacttg ttgcaataga
     4861 aactgtaaac ccatgctctt ttgcttcatt agttaatgta gaaagaatct ccatgaaaaa
     4921 gggttcactt aagcggtctg gtaaaaccag tggaggaaaa ataactccaa tattttgcgt
     4981 taaaccacta gccaacattt gtgctgcaac attaggaaca tagcccagct cttccatagc
     5041 ttttttcacg cgctctttcg tcttacttga aatagattca ttatctttta gtacacgact
     5101 tactgtcgat ggattaacac cagctttggc agcaacatct tttatagtta ccatcctacg
     5161 acctcttcat tctaatcatt ttataccttt tggtatagtc ctttgtctat tatactctaa
     5221 tctaagaaaa aaacaaacgt ttgcc
//
LOCUS       DATROD010000118         5040 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.120, whole genome shotgun sequence.
ACCESSION   DATROD010000118 DATROD010000000
VERSION     DATROD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.120"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            139..1687
                     /locus_tag="VBC74_001786"
     rRNA            139..1687
                     /locus_tag="VBC74_001786"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1742..1814
                     /locus_tag="VBC74_001787"
     tRNA            1742..1814
                     /locus_tag="VBC74_001787"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1775..1777,aa:Ala,seq:tgc)
     gene            1969..4871
                     /locus_tag="VBC74_001788"
     rRNA            1969..4871
                     /locus_tag="VBC74_001788"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            4946..>5040
                     /gene="rrf"
                     /locus_tag="VBC74_001789"
     rRNA            4946..>5040
                     /gene="rrf"
                     /locus_tag="VBC74_001789"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1427 a         1022 c         1453 g         1138 t
ORIGIN      
        1 agttgtctca agagaggcaa agacctttga aaactgaaca agacgaacga atgtgcaggg
       61 tggatttaca ttgtaaatcc gtcaatgaca aaaaacaata aatctgtcag cgagacagaa
      121 aagagcaaag ctcaaacttt taatgagagt ttgatcctgg ctcaggacga acgctggcgg
      181 cgtgcctaat acatgcaagt agaacgctga ggtttggtgt ttacactaga ctgatgagtt
      241 gcgaacgggt gagtaacgcg taggtaacct gcctcatagc gggggataac tattggaaac
      301 gatagctaat accgcataag agtaattaac acatgttagt tatttaaaag gagcaattgc
      361 ttcactgtga gatggacctg cgttgtatta gctagttggt gaggtaaagg ctcaccaagg
      421 cgacgataca tagccgacct gagagggtga tcggccacac tgggactgag acacggccca
      481 gactcctacg ggaggcagca gtagggaatc ttcggcaatg gacggaagtc tgaccgagca
      541 acgccgcgtg agtgaagaag gttttcggat cgtaaagctc tgttgttaga gaagaacgtt
      601 ggtaggagtg gaaaatctac caagtgacgg taactaacca gaaagggacg gctaactacg
      661 tgccagcagc cgcggtaata cgtaggtccc gagcgttgtc cggatttatt gggcgtaaag
      721 cgagcgcagg cggttcttta agtctgaagt taaaggcagt ggcttaacca ttgtacgctt
      781 tggaaactgg aggacttgag tgcagaaggg gagagtggaa ttccatgtgt agcggtgaaa
      841 tgcgtagata tatggaggaa caccggtggc gaaagcggct ctctggtctg taactgacgc
      901 tgaggctcga aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccgtaaa
      961 cgatgagtgc taggtgttag gccctttccg gggcttagtg ccgcagctaa cgcattaagc
     1021 actccgcctg gggagtacga ccgcaaggtt gaaactcaaa ggaattgacg ggggcccgca
     1081 caagcggtgg agcatgtggt ttaattcgaa gcaacgcgaa gaaccttacc aggtcttgac
     1141 atccttctga ccggcctaga gataggcttt ctcttcggag cagaagtgac aggtggtgca
     1201 tggttgtcgt cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga gcgcaacccc
     1261 tattgttagt tgccatcatt aagttgggca ctctagcgag actgccggta ataaaccgga
     1321 ggaaggtggg gatgacgtca aatcatcatg ccccttatga cctgggctac acacgtgcta
     1381 caatggttgg tacaacgagt cgcaagccgg tgacggcaag ctaatctctt aaatccaatc
     1441 tcagttcgga ttgtaggctg caactcgcct acatgaagtc ggaatcgcta gtaatcgcgg
     1501 atcagcacgc cgcggtgaat acgttcccgg gccttgtaca caccgcccgt cacaccacga
     1561 gagtttgtaa cacccgaagt cggtgaggta accttttagg agccagccgc ctaaggtggg
     1621 atagatgatt ggggtgaagt cgtaacaagg tagccgtatc ggaaggtgcg gctggatcac
     1681 ctcctttcta aggataagga aacctgccat ttgcgtcttg tttagttttg agaggtcttg
     1741 tggggcctta gctcagctgg gagagcgcct gctttgcacg caggaggtca gcggttcgat
     1801 cccgctaggc tccattgaat cgaaaggttc aaattgttca ttgaaaattg aatatctata
     1861 tcaaattcca cgatctagaa atagattgta gaaagtaaca agaaaataaa ccgaaaacgc
     1921 tgtgaatatt taatgagttt tctagtttta aagaaactag tttaataagg ttaagttaat
     1981 aagggcgcac ggtggatgcc ttggcactag aagccgaaga aggacgtgac taacgacgaa
     2041 atgctttggg gagctgtaag taagcgctga tccagagatg tccgaatggg ggaacccact
     2101 agttaatgac tagtatccat atctgttaag gatatgagaa ggaagacgca gtgaactgaa
     2161 acatctaagt agctgcagga agagaaagca aaagcgattg ccttagtagc ggcgagcgaa
     2221 acggcaggag ggcaaaccga agtgtttaca cttcggggtt gtaggactgc gatataggat
     2281 taatcattat agaagaatta cctgggaagg taagccaaag agagtaacag cctcgtattt
     2341 aaaatgatag attaacctta gcagtatcct gagtacggcg agacacgcga aatctcgtcg
     2401 gaatctggga ggaccatctc ccaaccctaa atactctcta gtgaccgata gtgaaccagt
     2461 accgtgaggg aaaggtgaaa agcaccccgg gaggggagtg aaatagaacc tgaaaccgtg
     2521 tgcctacaac aagttcgagc ccgttaatgg gtgagagcgt gccttttgta gaatgaaccg
     2581 gcgagttacg atatgatgcg aggttaagtt gaagagacgg agccgtaggg aaaccgagtc
     2641 ttaatagggc gccatagtat catgttgtag acccgaaacc atgtgaccta cccatgagca
     2701 ggttgaaggt gaggtaaaac tcactggagg accgaaccag ggcacgttga aaagtgcttg
     2761 gatgacttgt gggtagcgga gaaattccaa acgaacttgg agatagctgg ttctctccga
     2821 aatagcttta gggctagcgt cgatgttaag tctcttggag gtagagcact gtttgggtga
     2881 ggggtccatc ccggattacc aatctcagat aaactccgaa tgccaacgag atataatcgg
     2941 cagtcagact gcgagtgcta agatccgtag tcgaaaggga aacagcccag accaccagct
     3001 aaggtcccaa aatatatgtt aagtggaaaa ggatgtgggg ttgcacagac aactaggatg
     3061 ttagcttaga agcagctatt cattcaaaga gtgcgtaata gctcactagt cgagtgaccc
     3121 tgcgccgaaa atgtaccggg gctaaaacat attaccgaag ctgtggatga cacaaaagtg
     3181 tcatggtagg agagcgttct atgtgtgaag aaggtgtacc gtgaggagcg ctggaacgca
     3241 tagaagtgag aatgccggta tgagtagcgc aagacaggtg agaatcctgt ccaccgtaag
     3301 actaaggttt ccaggggaag gctcgtccgc cctgggttag tcgggaccta aggagagacc
     3361 gaaaggtgta tccgatggac aacaggttga tattcctgta ctagagtata tagtgatgga
     3421 gggacgcagt aggctaacta aaccagacga ttggaagtgt ctggtcaaac agtgaggtgt
     3481 gatatgagtc aaatgcttat atctttaaca ttgagctgtg acgaggagcg aagtttagta
     3541 gcgaagttag tgatgtcaca ctgccaagaa aagcttctag cgatacatat actctacccg
     3601 taccgcaaac cgacacaggt agtcgaggcg agtagcctca ggtgagcgag agaactctcg
     3661 ttaaggaact cggcaaaatg gccccgtaac ttcgggagaa ggggcgctga ttttagatca
     3721 gccgcagtga ataggcccaa gcaactgttt atcaaaaaca cagctctctg ctaaatcgta
     3781 agatgatgta tagggggtga cgcctgcccg gtgctggaag gttaagagga gtgcttagcg
     3841 taagcgaagg tatgaattga agccccagta aacggcggcc gtaactataa cggtcctaag
     3901 gtagcgaaat tccttgtcgg gtaagttccg acccgcacga aaggcgtaat gatttgggca
     3961 ctgtctcaac gagagactcg gtgaaatttt agtacctgtg aagatgcagg ttacccgcga
     4021 caggacggaa agaccccatg gagctttact gcagtttgat attgagtatc tgtaccacat
     4081 gtacaggata ggtaggagcc tatgataacg ggacgccagt ttcgtttgag gcgttgttgg
     4141 gatactaccc ttgtgttatg gctactctaa cccagatagg caatccctat cggagacagt
     4201 gtctgacggg cagtttgact ggggcggtcg cctcctaaaa ggtaacggag gcgcccaaag
     4261 gttccctcag actggttgga aatcagtcgt agagtgtaaa ggtataaggg agcttgactg
     4321 cgagagctac aactcgagca gggacgaaag tcgggcttag tgatccggtg gttccgtatg
     4381 gaagggccat cgctcaacgg ataaaagcta ccctggggat aacaggctta tctcccccaa
     4441 gagttcacat cgacggggag gtttggcacc tcgatgtcgg ctcgtcgcat cctggggctg
     4501 tagtcggtcc caagggttgg gctgttcgcc cattaaagcg gcacgcgagc tgggttcaga
     4561 acgtcgtgag acagttcggt ccctatccgt cgcgggcgta ggaaatttga gaggatctgc
     4621 tcctagtacg agaggaccag agtggactta ccgctggtgt accagttgtc ttgccaaagg
     4681 catcgctggg tagctatgta gggaagggat aagcgctgaa agcatctaag tgcgaagccc
     4741 acctcaagat gagatttccc atgattttat atcagtaaga gccctgagag atgatcaggt
     4801 agataggtta ggagtgtaat tgtggtgaca catttagcgg actaatacta atagctcgag
     4861 gacttatcca aaaagagata ttgacaacgt tacggtttct tgttagacta tagatattca
     4921 attttgagtg aataagactc aatagttaag tgataattgc ctaggagata cacctgttct
     4981 catgccgaac acagaagtta agccctagaa cgccggaagt agttgggggt tgccccctgt
//
LOCUS       DATROD010000119         5015 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.121, whole genome shotgun sequence.
ACCESSION   DATROD010000119 DATROD010000000
VERSION     DATROD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.121"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..578)
                     /locus_tag="VBC74_001790"
     CDS             complement(<1..578)
                     /locus_tag="VBC74_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942710.1"
                     /translation="MKKLIWSHLFAIVSILGIWYYHVKIDHLMVILLASVLSLEMGFI
                     AYYSRNIFIRLLASLGLLFFYPQSLGIFLSLVSLEWSTKDIWQSNHFMQYMAYLLAIL
                     FIISSALLSLKLLVNAYDISKRYQLATVIPVMLFSSFAFCLEKSGSLGLEGIILNPSA
                     FFNGIQTNMANLDFKALSCLFLVQLLLWGILM"
     gene            667..1479
                     /locus_tag="VBC74_001791"
     CDS             667..1479
                     /locus_tag="VBC74_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEO6942711.1"
                     /translation="MTKKIIAVDLDGTLLHNNNTISDYTADTLRKVQAQGHKVIITTG
                     RPYRMALAHYLRLDLKTPMINFNGALTHIPEKKWAFEHSATIDKKLLLETLNLSDAIQ
                     ADFIASEYRKNFYITMDNRDKINPQLFGVNEITDKMALDVTKITRNPNALLMQTRHKD
                     KYELAKELRQHFNHELEVDSWGGPLNILEFSPKGVNKAYALKHLLKSLNLSQENLIAF
                     GDEHNDTEMLAFAHTGYAMKNASPTLLPYADQQIQWTNEEDGVAKTLEKLLL"
     gene            1781..2791
                     /gene="manX"
                     /locus_tag="VBC74_001792"
     CDS             1781..2791
                     /gene="manX"
                     /locus_tag="VBC74_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEO6942712.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIAQFDADDEVLVLADLWSGSPFNQASRVMGENPERKMAIITGLNLPMLIQ
                     AYTERMMDANAGVEQVAANIIKESKEGIKALPEELNPVVEATPVAGVPADVPAEVKQS
                     GSIPEGTVIGDGKLKINLARIDTRLLHGQVATAWTPASKANRIIVASDEVSKDELRKQ
                     LIKQAAPGGVKANVVPISKLIEVAKDPRFGNTRALILFETVQDALRAVEGGVEIPELN
                     VGSMAHSTGKTMVNNVLSMDKDDVAAFEKLRDLGVSFDVRKVPNDAKKNLFDLINKAN
                     VK"
     gene            2824..3636
                     /locus_tag="VBC74_001793"
     CDS             2824..3636
                     /locus_tag="VBC74_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEO6942713.1"
                     /translation="MADISIISMILVVIVAFFAGLEGILDQFQIHQPLVACTLIGLVT
                     GHLEAGIILGGSLQMIALAWANIGAAVAPDAALASVAAAIILVKSGDFTQKGINFAFS
                     TAVPLAIAGLFLTMIVRTISTALVHAGDKAASEGNFAAIERFHFIALLLQGLRIAFPA
                     ALLLAIPSSSVQSILEAMPDWLNGGMQVGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAALNQLTLIAMGTIGVAIALIYISLSKMGGSKGTSNAGSNDPIGDILEDY"
     gene            3651..4562
                     /locus_tag="VBC74_001794"
     CDS             3651..4562
                     /locus_tag="VBC74_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEO6942714.1"
                     /translation="MTEQIKLSKSDRQKVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTTKEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEEKASGTPVEDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASAGNILGPIIFFVGWNLIRMSFLWYTQE
                     LGYKSGKEITKDMSGGILQDITKGASILGMFILAVLVKRWVAINFTVDLPKKTLSEGA
                     YINFPKDHVSGQQLHDILGQVQSGLSLDKMQPQTLQGQLDSLIPGLAGLLLTFFCMWL
                     LKKKVSPITIIIGLFIVGILARLAGVM"
     gene            4681..>5015
                     /locus_tag="VBC74_001795"
     CDS             4681..>5015
                     /locus_tag="VBC74_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEO6942715.1"
                     /translation="MAQSLNKTVEFQTTGVSYLGMGNKVGKFLVGDQALEFYNDKNVN
                     DYIQIPWTSINQIGANVSRKKISRHFEVFTDQGKFLFASKDSGTILKHARRHIGDDKV
                     VKLPTLIQT"
BASE COUNT         1618 a          936 c          902 g         1559 t
ORIGIN      
        1 tccattaata ttccccaaag taataattgt actaaaaaaa ggcaagacaa ggctttaaaa
       61 tcaaggttag ccatattagt ctgtattcca ttaaaaaatg ctgacggatt taaaatgatt
      121 ccttctaaac ctaatgatcc tgatttttct aagcaaaagg caaaactact aaaaagcatt
      181 acaggtataa cagttgctaa ttgatacctt ttactaatat catatgcatt taccaatagc
      241 ttcaaactta gtaaagctga agagataata aaaagaattg caagaaggta tgccatatat
      301 tgcataaagt gattagactg ccaaatgtct tttgttgacc actctaagct cactaaacta
      361 agaaaaatcc ctaaactctg tgggtaaaaa aataaaaggc ctaaacttgc taaaagacga
      421 ataaaaatat tacgactata ataagcaatg aatcccatct ctaacgacaa gactgatgcc
      481 aatagaatta ccatcaaatg atcaattttt acatgataat accatatccc taaaatactg
      541 actatggcaa aaagatgact ccaaattaac tttttcataa tccaccaact tccatatttc
      601 cacagttatt atatcatatt tctactgtct ctttttattg aaatctattt ttgatataat
      661 gtcagcatga caaagaaaat tatcgccgta gatttagacg gtactctatt acataataat
      721 aacactattt ctgactacac cgctgataca cttcgaaaag ttcaagctca aggacacaaa
      781 gtaattatta caaccggacg cccataccgt atggcactcg ctcattattt acgcctagat
      841 ttaaaaacac caatgataaa ttttaacggt gctttaactc atatccccga aaaaaagtgg
      901 gcgtttgagc atagtgcaac gattgataaa aaattactcc ttgaaacctt aaatttatca
      961 gatgctatcc aagccgattt tattgctagc gagtaccgta aaaattttta cattactatg
     1021 gataatcgtg ataaaattaa cccacaatta ttcggtgtca atgaaattac tgataaaatg
     1081 gcattagatg ttactaaaat cacacgcaat ccaaatgctc tattgatgca aacacgtcat
     1141 aaagataagt atgagttagc taaagagtta cgacaacatt ttaatcacga actagaagta
     1201 gattcttggg gtggtccatt gaatatactc gaattttcac ctaaaggtgt taacaaagct
     1261 tatgctctca aacatttatt aaaatcactc aatctttcac aagaaaattt gattgccttt
     1321 ggagatgaac acaatgacac cgagatgctg gcctttgcac atacaggata tgctatgaaa
     1381 aatgctagtc caactctact tccatatgcc gaccaacaaa tccaatggac aaacgaagaa
     1441 gatggtgttg ctaaaactct agaaaaatta ttattgtagt attttcaaaa acgatctcta
     1501 ttgaaacaga attctaatag aaatcgtttt ttaggttttc tactatatta ttaacaaaaa
     1561 aagttaccac tctcttcccc cattataaaa ggtatttgtg aaaaaaatat ctttttttta
     1621 atttttatta gaaatagtat tgattttttt ctgaaaacgt tttaaaatat atctgttcta
     1681 taatttcgtt gaaatgaaaa agaatggcca attttacgat tggaaggaga ttattcttct
     1741 ttatgtcaac gaaaaaattt tttaaggagg aagaacaaga atgggtatcg gtattattat
     1801 tgccagccat ggtaaatttg ctgaaggtat tcatcaatca ggttcaatga tctttggtga
     1861 acaagaaaaa gttcaagttg taactttcat gccaaatgaa ggaccagatg atttgtatgg
     1921 acacttcaac aatgctatcg cacaatttga tgctgatgat gaggtgcttg tgttagctga
     1981 cctttggagt gggtcaccat ttaaccaagc aagccgtgtt atgggagaaa atcctgaacg
     2041 taaaatggct atcatcactg gtctcaattt gccaatgctt atccaagcct atactgagcg
     2101 tatgatggac gctaatgcag gtgttgaaca agttgccgca aatattatta aagagtctaa
     2161 ggaaggtatc aaagcccttc cagaagaact taatcctgtt gtagaagcta ctcctgtagc
     2221 aggtgttcct gcagatgttc ctgcggaagt taaacaaagt ggatctatcc ctgaaggaac
     2281 tgttattgga gatggcaagc ttaaaattaa tcttgctcgt attgacacac gtcttttaca
     2341 tggtcaagtt gcaactgcat ggactccagc atcaaaagca aaccgtatta tcgttgcttc
     2401 tgatgaagtt tctaaagatg aacttcgtaa acaattgatt aagcaagctg ctcctggagg
     2461 agtcaaagca aacgttgtcc caattagtaa attaattgaa gtagctaaag acccacgttt
     2521 cggaaacaca cgtgccttga tcttatttga aactgtacaa gatgcactcc gtgcagttga
     2581 aggtggtgtg gaaatccctg aattgaacgt tggttcaatg gctcactcaa ctggtaaaac
     2641 aatggtaaac aacgttcttt caatggataa agacgatgtt gctgcctttg aaaaattacg
     2701 tgacttaggt gtatcatttg atgtacgtaa agtaccaaat gacgctaaaa agaatttgtt
     2761 tgatttaatc aacaaagcaa acgtaaaata acttattatc tctgaaaacg aaaggatatc
     2821 aaaatggcgg atatttcaat tatctcaatg attttggtcg ttatcgttgc cttctttgct
     2881 ggtcttgaag gtatcctcga ccaattccaa atccatcaac ctcttgttgc ttgtacttta
     2941 atcggtcttg taactggtca cctcgaagcg ggtattattc ttggtggtag tctccaaatg
     3001 attgctcttg cttgggcaaa tatcggagct gccgtagcac cagacgctgc acttgcctct
     3061 gttgctgctg ctattatttt ggttaaaagt ggcgacttca ctcaaaaagg tatcaacttt
     3121 gccttctcta ctgctgtacc tctagctatt gctggacttt tccttacaat gattgttcgt
     3181 acaatctcaa ctgcacttgt tcacgctggt gacaaagctg cttctgaagg aaacttcgca
     3241 gcaattgaac gttttcactt tattgccctc cttctccaag gattacgtat tgcattccca
     3301 gctgcgcttc ttttagctat tccatcttct tcagtacaaa gtattcttga agcaatgcct
     3361 gattggttaa atggtggtat gcaagttggt ggtgccatgg ttgttgccgt tggttacgct
     3421 atggttatca atatgatggc tactcgcgaa gtttggcctt tctttgctct tggatttgct
     3481 ctagcagctt tgaaccagtt aactcttatt gcaatgggta ctattggtgt tgctatcgcc
     3541 ctcatctaca tcagtttatc aaaaatgggt ggaagcaaag gtacttctaa cgcaggttct
     3601 aacgatccta tcggcgatat cttagaggac tactagaaag gagctaaaca atgactgaac
     3661 aaattaaatt atcaaaatca gatcgtcaaa aagtttggtg gcgttcacaa ttccttcaag
     3721 gttcttggaa ctatgagcgt atgcaaaata tggggtgggc ctacgctctt atcccagcat
     3781 tgaaaaaact ttacactact aaagaagatc gtgctgctgc gcttgagcgc catatggaat
     3841 tctttaacac acatccatac gttgctgctc caattatcgg tgtaaccctt gctcttgagg
     3901 aagaaaaagc aagcggtaca cctgtcgaag acaaagcaat tcaaggggtt aaaatcggta
     3961 tgatgggacc tcttgctggt attggtgatc cagtattctg gtttactgta cgtcctatct
     4021 taggtgctct tggtgcttca cttgcctctg ctggtaatat cctcggacca atcatcttct
     4081 ttgttggttg gaacctcatt cgtatgtcat tcttatggta tacacaagag ttaggttaca
     4141 aatcaggtaa agaaattact aaagatatgt ctggcggtat cctccaagat attactaaag
     4201 gtgcttcaat ccttggtatg ttcattcttg ctgtccttgt taagcgttgg gtagcaatta
     4261 actttactgt agaccttcct aaaaagacat tgtcagaagg ggcatacatc aatttcccta
     4321 aagaccatgt tagtggacaa caattgcatg atatcctagg acaagttcag agtggcttaa
     4381 gccttgataa aatgcaacca caaacacttc aaggacaact tgactcactt attcctggtt
     4441 tagctggatt acttcttaca ttcttctgta tgtggttact taagaagaaa gtatcaccaa
     4501 ttacaattat catcggtttg tttatcgttg gtattctagc tcgccttgca ggtgtgatgt
     4561 aataaaacta agagatggtt acaatagtat tccatctctt atattttatt accaaaaatc
     4621 cacttagagt cccactatta tgatataatc aaactactat aaattaaaaa gggattagtc
     4681 atggcacaat cattaaacaa aactgttgaa tttcaaacaa caggagtttc gtatttaggt
     4741 atggggaata aagttggaaa atttttagtt ggtgaccagg cattagaatt ctataacgat
     4801 aaaaatgtca acgactatat tcaaattcca tggacatcaa tcaatcaaat tggagctaat
     4861 gttagtcgaa aaaaaattag tcgtcacttt gaggtcttta cagaccaagg aaaattttta
     4921 tttgcctcta aagactctgg aacgatatta aagcatgcga gacgacatat tggagacgat
     4981 aaagttgtta agcttcctac cctcatccag accat
//
LOCUS       DATROD010000120         4935 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.122, whole genome shotgun sequence.
ACCESSION   DATROD010000120 DATROD010000000
VERSION     DATROD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.122"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(70..978)
                     /locus_tag="VBC74_001796"
     CDS             complement(70..978)
                     /locus_tag="VBC74_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6942716.1"
                     /translation="MKTIKDWQINICLATYNGQKYLRQQLDSIIQQGYTDWICLIRDD
                     GSTDDTVAIIKEYVNRDSRFIFINSNDDRKLGSHRSFYELVNYKKADFYVFSDQDDVW
                     KENRLERYLEEAEKFNQELPLLVYSNWTSVDEKLTVLKEHNPATVIQEQIAFNQINGM
                     VIMMNHELAKLWEYRQIGAHDSYVGTLAYAVGNVAYISDSTVLWRRQVGAESLNNYGR
                     QYGVATFWQMINTSFDRASLIFAQVSDKMSLERKLFFSRFIELKNANLIRRIYLLSKL
                     KLRRKSLKETVAMTILLLTGYGKPKA"
     gene            complement(1188..4888)
                     /locus_tag="VBC74_001797"
                     /pseudo
     CDS             complement(1188..4888)
                     /locus_tag="VBC74_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646417.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
BASE COUNT         1476 a          966 c          734 g         1759 t
ORIGIN      
        1 ctttttgaaa aataaatatt ttctatattt ttaactacaa ctcaacttga tttccttcaa
       61 ccaaaaaaat tatgcttttg gtttaccgta acctgttaaa agtaggatag tcatagcaac
      121 tgtttctttc aatgatttcc gacgaagttt taattttgat aaaagataaa tccttcttat
      181 tagattggca ttttttaatt ctataaaacg actaaagaag agtttacgct ccagggacat
      241 tttatcggat acttgagcaa aaataagcga agcacgatca aaagaagtat ttatcatctg
      301 ccaaaacgta gccaccccat attgtctccc gtaattattt agcgactctg cccctacttg
      361 tctacgccaa aggactgtac tgtcagaaat ataagccaca tttccaacgg cgtaagccag
      421 agttccaaca taagaatcat gggcgccaat ttgacggtat tcccaaagct ttgctaactc
      481 atgattcatc atgataacca taccatttat ttggttaaag gcaatttgtt cttgaataac
      541 tgttgctgga ttatgttctt tcaatactgt taatttttca tctacagaag tccagttcga
      601 ataaacaagc aatggtaatt cttggttaaa tttctctgct tcttcaaggt aacgttctaa
      661 acgattttct ttccaaacat catcctggtc tgagaaaaca taaaaatctg cttttttata
      721 gttaaccaac tcgtaaaaac tgcggtgact tcctaacttt ctatcatcgt tagaattaat
      781 aaatataaaa cgtgaatctc tattaacata ttctttaata atagctactg tatcatcagt
      841 agatccatcg tcccttatta aacaaatcca gtcagtataa ccttgttgta ttatgctatc
      901 taattgctgg cgaaggtatt tttgaccatt ataggtcgct aagcaaatat ttatttgcca
      961 atcttttatc gttttcattg ctatcaaaac tccttaaggc tagttcatct tatgaaagct
     1021 tacaaagcga acagtatcac atactaatcg taattataat attataatat tatatgccgt
     1081 taatcaatat aaaattaatc aaaaataaga gtcaataccc taaaagtcca gcttaacata
     1141 atgttaaact gaacttttca tcatattacc gagtagttta cttagtttta tttactatta
     1201 tttttagtct ttttgggttt caagtaaaga agtaaactag ataacaatgc caaagtgcca
     1261 acaaatagac cattcgttgc tctatctgat gtactaggta aggtatggtt aacagaatcc
     1321 ccgttatgtt taggtgatat gatcttagct actctgctac tattattgtt tgtagaagtg
     1381 aggactttct catttcctga tttagttatt tctttagaca atgattgagt agtaactgta
     1441 gtttgaggct taactaatgt tatttcttct agattcttgt ttactacttt tccttcttta
     1501 gaaataaaca taggttcttt cttcaagtta tcaaaatatt tgaaactgtt agaaattact
     1561 atagcgtttt ctttctctga tactactgcc ttattcaaga ggtcagacaa ttttaccgtt
     1621 gcaaaattac cagctttatc ttccacaaca agtgtcaagg ccttagggtc taccgcaacc
     1681 tcactctctc ctatcttaac cgttttagga agtgtcactt taccttcttg atctatatgg
     1741 aaataatggt aagaagtctc atccccatat tcttcatctt ttacaacatg agataaaact
     1801 aattgtaaac gatatgtagg aacataacta ctttccttag gcatggctaa gcttaatgtt
     1861 cgattagttt catcaaactg agctcgtgaa ggaagatttg gtgacttagt acttacttga
     1921 actttaaagt ctgactcctg actatttgct ccttctgcta ctggtgtgta acgtaagcga
     1981 taagtataaa aaccatctgc tacaactttg ccatccttat ctaaaccact ccactgaaga
     2041 gcatccatac gataatgacc atcactttgc tttggattat tatggaaatt tttacgataa
     2101 gatggtaaaa ccttactttg ccaaataaca tttccatttt gatctagaac ttgagcagaa
     2161 atatccttaa catttcttaa gaaagttgcc tggggagtga tttcgtccct atttccatct
     2221 ttatttggag aaatggcaaa atatggatta ttcgctgcat ctctttccaa aagatgaatt
     2281 gttttatcct caaccttatt ctcaaaagtt cctaaaataa ttctttttgg actctccggt
     2341 gctaattcta actccccacc atttttgaca taatcaacat agccccaaga cgctgattgt
     2401 gttaacaagg cagtatagtt gttgctttca aaaggagctg attcattgta ctccaattgg
     2461 tctttatgag ttgtatcatt tggtttatag tagaaactac ctttagaaag cgtcttataa
     2521 atcggtgttt caagtgcttg taagttcgca aaatcaccat taaatcctac aaaaggaata
     2581 ctcattaact cctgattact atccttggct tctttaaaac gtacaaaacc ttctaagaaa
     2641 taaccatttg ccatctgttc ttttaatttc tgactaaatt gactagcatc aatagtaaat
     2701 cgaacttgtg tttctttatc acgaagaatt actttctgcc aattagtatc tagcaaggct
     2761 tgtggtttaa gggcaaattt acctttattt acttgttctg ttgctacatt agcttgataa
     2821 tacaattctt tgacaccttc tacaagttta tgaattgtaa ctgtgatatc aaatttatct
     2881 ccctctcgtt tgagattaat tttaactttg ccatcgtttc cagtaacata atattgagct
     2941 tggatagctt tttcagcatc aactacacct gcaccttgct gacgtggtga ataaaacgcc
     3001 ttatcctctt cactatataa tgctgttgct gagctcatga ggatgttttt agacaattct
     3061 agcaattttt tagaatctaa attcatccct ttatatttct cagccaaatg actttgaagc
     3121 attgtcatta atcctgcaac atgtggtgaa gccatacttg taccagacat tgtttagtat
     3181 tgattattat aggttgaaga ataaatttca aagccagaag ctgttacatc aggcttgatt
     3241 gctccttcag ctgtcacgcc ccaacttgat tgttccagca tacgattgcc accttggcta
     3301 tcaactactt caaaactctg gttaaatgtt aactgacttg aagtattttt tatacgctcg
     3361 ccatctactt tactaataac ccccacaggt aattcacggt aaggaattag aaaatttcca
     3421 cgtttttctt gatcgttaaa aataacgata ccaacaacac ctgcatttgt agcatgagtg
     3481 attttagtca taaaatcaag tccaccacca cgctcaatta atgcaatctt acctttaaag
     3541 tccttacctt caaaatcttt tttgcaccat aattggcata aaccacatcg taggccttac
     3601 ctttgtcaaa aggtttagaa gtcacaatcg gcaacttaac taacttacct tcaatagttg
     3661 tttcaacgac ctcactgata gttttaagtg attcatagct agcaacactc aaagtatctt
     3721 cagaaatagc tggactatta accgtaccgt agtcaggatt agttgataat ggtttgctat
     3781 aatccatacc aaatgcacct tcatttccgg cagccacaac aactgcaacg cccttctcag
     3841 aagctaattt aagtgctaat ttaactttat cattgagagc aattaaagaa tcagccgttt
     3901 ttcccaggct catattaatc gtttttgctc ctagattaac agcgtctatg attgctttag
     3961 catatgcttc tccaaatttg tccgaatcaa ttttatctgg aatacgcatt aataagactt
     4021 gagcatttgg cgctgcacct tctaaaagaa gactattgat tgctggacgt ttactattac
     4081 ctacaaaaat accagcaacg tgtgtaccat gcaaaatatt ctttgcttct gacccataac
     4141 catctttcat agctgctgca atatcagcca ccgtttctgt attgttggcg tagttatgtg
     4201 caaaaacaat cttatcgtta acccatttcc cataagtgat attatgtttt gcttttaatt
     4261 cctcaaattc tgccttagtt ttaaagctgt gcttatcatc ttttgggcta tctaaacgaa
     4321 aaatatcatg gttaatatca aagccagtat caataatagc tactacattt ccctttcctc
     4381 tttgggttgt ggcttgtaat tttgttattt tagatgtatc aagaacagca agcgatgact
     4441 tgctttctga cttcacctct tcatatgctg agggaacttt ctgtgctatt gcagttgaag
     4501 catttgttac tacattgctt tctttattgt tggttgtctc tggtttagag ggatattctt
     4561 cttcaagatc agcccccaaa ttagacgtat caaggttttt agataactct tctaataatt
     4621 catcttctgt tttgtcattt tttacagatg tatcacccat ttctttcact gtattactag
     4681 cagaagcagc tgttacagat gttttttcaa caatattagt agttaccgat ggcgatggct
     4741 gttgagcaac attagcaatt acaggtgatt gctcttggtt ttttaattct tgctcctctg
     4801 cattcacttg attgctatgc attaataaaa tactagttgt tataagtgtt aactttaaaa
     4861 tagccttttt tgagtgatgt ttatccactt ttcctatttc ctccaaaaat cagtatgcat
     4921 actatattac aacat
//
LOCUS       DATROD010000121         4818 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.123, whole genome shotgun sequence.
ACCESSION   DATROD010000121 DATROD010000000
VERSION     DATROD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.123"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(20..1216)
                     /locus_tag="VBC74_001798"
     CDS             complement(20..1216)
                     /locus_tag="VBC74_001798"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEO6942717.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILEQDPDAHVAAE
                     TAVYTGSVHVFGEISTTAYVDINRVVRNTIAEIGYDKAEYGFSAESVGVHPSLVEQSP
                     DIAQGVNEALEVRGSLEQNPLDLIGAGDQGLMFGFAVDETPELMPLPISLAHQLVKKL
                     TDLRKSGELTYLRPDAKSQVTVEYDDNDQPIRVDAVVISTQHDPNVTNDQLHKDVIEK
                     VINEVIPSHYLDDQTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKVEVQLAYAIGVAQPVSVRVDTFGTGVIA
                     EADLEAAVRQIFDLRPAGIINMLDLKRPIYRQTAAYGHMGRTDIDLPWERVDKVQALK
                     DFIASK"
     gene            1401..2336
                     /locus_tag="VBC74_001799"
     CDS             1401..2336
                     /locus_tag="VBC74_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEO6942718.1"
                     /translation="MKTYEKIYQILSKNNNFISGETMANQLNISRTAIWKGIKTLEEL
                     GLEIESVTNKGYRLVSGDILLPEQLEQEIGIKVSLNNNSASTQLDAKMGIESKLKTPH
                     LFLAPNQKKAKGRFDRPFFTSNQGGIYMSLLLQPNVPIEDIKPYTVMVASSAVKAISR
                     LTGITPEIKWVNDIYLDNKKIAGILTEAIASVESGLVTNVIIGLGINFYIKEFPRALT
                     KRAGSLFTEQPTITRNQLITEIWNLFFNIPLEDHLKVYREKSLVLDKTVSFMDGQTMY
                     SGKAIDITDKGYLVVELDDGQLKTLRSGEISLSSW"
     gene            complement(2317..2511)
                     /locus_tag="VBC74_001800"
     CDS             complement(2317..2511)
                     /locus_tag="VBC74_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEO6942719.1"
                     /translation="MTKRQFIFMALLCSFETYFFNQSVMDGSWIFAIFWGVLLLRDLQ
                     KVYAISKFTKELIKSTKKKD"
     gene            complement(2600..4264)
                     /gene="dnaX"
                     /locus_tag="VBC74_001801"
     CDS             complement(2600..4264)
                     /gene="dnaX"
                     /locus_tag="VBC74_001801"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEO6942720.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVSSKKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVNGEPCNHCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKLLAIRDHLAQILDKEAISYDLDALTLVARRAEGGMRDALS
                     ILDQALSLAKDNHISLDVAEEITGSISLSAIDDYVSNILAHDTTEALAKLEVIFDSGK
                     SMSRFATDLLMYLRDLLVVQAGGENSHSSDTFMANLDVKQDILFEMIDKVTSVLPEIK
                     NGSHPKVYAEMMTIQLSEMVEKNSSNIPADVTAELDSLRRELKSLKNEMSQLSRADQS
                     SSTQKVKVNNKTFTFKVDRTKILTIMEETVVDSQRSREYLEALKSAWNEILDNITAQD
                     RALLMGSEPVLANSENAILAFDAAFNAEQAMKRTDLNDIFGNIMSKAAGFSPNILAVP
                     RNDFNQIRSDFAKKMKAQKTETEPEVNHQIPEDFSYLAERIAIVED"
     gene            complement(4264..4761)
                     /locus_tag="VBC74_001802"
     CDS             complement(4264..4761)
                     /locus_tag="VBC74_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEO6942721.1"
                     /translation="MNKSKKIENYQLLLLQAQALFSDETNALANLSNASAMLNAMLPN
                     SVFTGFYLFDGEELILGPFQGGVSCVHITLGKGVCGESAQTAKTLIVDDVTKHANYIS
                     CDSKAMSEIVVPMFKNGKLLGVLDLDSSLVADYDEIDQEYLEKFVGILVEHTIWNLDM
                     FGVEK"
BASE COUNT         1551 a          934 c          789 g         1544 t
ORIGIN      
        1 ctttcgttta cgctttttat tatttgctag caataaaatc ctttaaggcc tggactttat
       61 caactcgctc ccatggcaaa tcgatatcag tacgtcccat atgcccatag gctgctgttt
      121 gtcgataaat aggtcgtttg aggtccaaca tattaataat gcctgctggt cgcaaatcaa
      181 atatttggcg tacagctgcc tctaggtctg cttctgctat aacacctgtt ccaaatgtat
      241 ctacacgcac agagacaggc tgagcaacac caattgcata agcaagttgt acttctactt
      301 ttttagctaa atcagctgca acaatatttt ttgcaatata acgagctgca taagatgctg
      361 aacgatctac tttagtagca tctttacctg aaaatgcacc accaccatga cgagaataac
      421 caccataagt atctacaata atcttacgac ctgttagtcc tgaatctcct tgagggcctc
      481 caataacaaa gcgccctgta ggattgataa agaattttgt ttggtcatct aaataatggc
      541 ttggaataac ttcattgata actttctcaa tcacgtcctt atggagttgg tcattagtta
      601 cattaggatc atgctgtgtc gaaataacta ctgcatccac acgaattggt tgatcattat
      661 catcatattc aaccgttact tgagattttg catctggtcg aagataggtg agttccccag
      721 acttacgtag atctgtcaat ttcttaacta actgatgagc taaagaaatt ggcaatggca
      781 ttaactcagg tgtttcatca acagcaaacc caaacatcaa accttggtca cctgcaccaa
      841 ttaaatctaa aggattttgt tcaagagaac cacgtacctc aagagcttca ttgactccct
      901 gtgcaatgtc tggcgactgt tcaacgagtg acggatgcac tccgacagat tcagctgaga
      961 agccatattc agctttatca tagccaattt cagcgattgt gttacggaca actcggttaa
     1021 tatcaacata ggctgtcgtt gatatctcgc caaatacatg aaccgaacct gtataaacag
     1081 ctgtttcggc tgctacgtgg gcatctggat cttgctctaa aattgcatct aaaatcgcat
     1141 cggaaatttg gtcagcaatc ttatcgggat gtccctcaga tactgactca gacgtgaaaa
     1201 gtttacgttc tgacataaaa atgtcccccc taaaatgtaa aatgtgtcga gttacaaaga
     1261 actagcctct aggctacctt ttcgggactc atatagctat tgtaccacta tttcatcttt
     1321 tgtgaaataa gttaccaata aataaaaaat tatctctatt ttctttagtc ttttttctcg
     1381 atttatacta tactagattt atgaaaacct acgaaaaaat ttatcaaata ctatcaaaaa
     1441 ataacaactt tattagcgga gaaactatgg caaatcaact taatatttcc cgcacagcta
     1501 tttggaaagg tataaaaacg ttagaagaat taggcctaga gattgagtct gtcactaaca
     1561 agggctaccg attggtatca ggtgacatat tattaccaga acaactcgaa caagaaattg
     1621 gtattaaggt tagtcttaat aataatagtg cttcaaccca actagatgct aaaatgggga
     1681 tagaaagcaa attaaagact ccacaccttt ttttagctcc taatcaaaaa aaagctaaag
     1741 gacgttttga tagacctttc tttacatcaa atcaaggcgg catctatatg tctcttttat
     1801 tacaacctaa tgtaccgatt gaagatataa aaccttatac tgtcatggta gcctctagtg
     1861 ctgtaaaagc tatctcacgg ttaacaggaa ttactcctga aattaagtgg gtcaatgata
     1921 tttatttaga taataaaaaa atagctggta ttttaaccga ggctatcgct tctgttgaga
     1981 gtggattggt aacgaatgtt attataggat taggtatcaa tttctatatc aaagagttcc
     2041 ctagggcgtt aactaagcgc gcaggtagtc tctttacaga gcaaccgacc ataactagaa
     2101 atcaattgat tacagaaatc tggaatctct ttttcaatat tccactagag gatcacttaa
     2161 aggtatatag agaaaaatcc ctagtcttag ataaaaccgt tagctttatg gatggtcaaa
     2221 caatgtattc tgggaaagct attgatatta ctgataaagg atatttagtg gttgagttag
     2281 atgatggtca gttgaaaaca cttcgcagtg gcgaaatcag tctttcttct tggtagattt
     2341 gataagctct tttgtgaatt ttgaaatcgc atacaccttt tgaagatctc gaagtaataa
     2401 aactccccaa aaaatagcaa aaatccagga gccatccata acggattgat tgaaaaaata
     2461 agtttcaaaa gagcataaaa gcgccataaa aataaattgt cgttttgtca tatcaccatt
     2521 ataacagaat ctactaaata attaaaaaac ttatcaaaaa aatagttgcc tgttacgggc
     2581 aactattttt agttacgttt taatcttcaa cgattgctat tcgttctgct agataagaaa
     2641 agtcttctgg aatctgatga ttaacttcag gttctgtttc cgtcttttga gctttcattt
     2701 tctttgcaaa atcgctccga atttgattaa aatcatttct aggtacagct aaaatattag
     2761 gtgaaaaacc tgctgcttta ctcataatat taccaaatat atcattgaga tcggtacgtt
     2821 tcatagcttg ttcagcatta aaggccgcat caaatgccaa aatagcattc tcgctattag
     2881 ctaagacagg ttcagatccc attaataagg ctctatcttg agccgtaata ttatctaata
     2941 tctcgttcca agcactcttt aaagcttcta aatattcacg tgaacgttgg ctatctacaa
     3001 ccgtttcttc cataatggtc aaaatctttg tacggtcaac tttgaatgtg aatgtcttat
     3061 tattaacctt tactttttga gttgatgaac tttgatctgc tcgagagagt tggctcattt
     3121 cgttttttaa cgattttaac tctcgacgaa gactatctaa ttctgctgtc acatctgcgg
     3181 gtatgtttga cgaatttttt tctaccattt cagacaattg aatggtcatc atctctgcat
     3241 aaactttagg atggctacca ttttttatct caggaagaac agatgtcact ttatcaatca
     3301 tttcaaataa aatatcttgt ttaacatcaa gattggccat aaaagtatca cttgaatgac
     3361 tattttcacc acctgcttga acaacaagta aatctcttaa gtacatcaat aaatctgttg
     3421 caaaacgact catacttttg ccactatcaa agattacttc aagtttagcg agtgcttcag
     3481 ttgtatcgtg agccaatata ttcgatacat aatcatctat agcagataag gaaatgctcc
     3541 ctgtaatttc ttctgctaca tctaaagaaa tgtggttatc tttcgctaaa ctcaaggctt
     3601 gatcgagaat tgataaagca tcacgcatac caccttcggc tcgtctagcg actagtgtca
     3661 gagcatccaa atcatatgaa atagcttctt tatctaaaat ctgtgctaaa tgatctcgaa
     3721 tagctaataa tttaatcgct ttaaattcaa agcgctggac acgagataag attgttgcag
     3781 ggattttatg gagttctgtt gttgctaata taaaaacaac attttctgtt ggctcttcaa
     3841 gtgttttcaa tagtgcattg aaggcacctg tagacaacat atgaacttca tcaataatat
     3901 aaactttata ggttgcccta cttggagcat aagttgattt gtccctgata tcacgaattt
     3961 catcgacacc attatttgac gcagcatcaa tttcaatgac atcctctaaa ctaccatttg
     4021 taatatctcg acaaatatca caatgattac aaggttcgcc attaacttga tttgggcaat
     4081 tcatcgcttt tgcaaatatt ttagcagcac ttgtttttcc agttccccta ggtcctgaaa
     4141 aaaggtaagc atggctaatt tttttgctgg acacagcttg ctttaaagta gttgaaataa
     4201 cactttgtcc aaccatttca tcaaatgttt ggctacgata tttccgataa agggcctgat
     4261 acattacttt tcaactccaa acatatccaa attccaaatc gtatgttcta ctagaatacc
     4321 tacaaatttt tctaagtatt cttgatcaat ctcatcataa tctgctacta aagaagaatc
     4381 taaatctaga actcctagaa gtttgccatt tttaaacatg ggtactacga tttcactcat
     4441 agcttttgaa tcacaggaga tatagttagc atgctttgta acatcatcaa caatcagcgt
     4501 cttagcagtt tgtgcagatt caccacaaac accttttcct aaagtaatat gcacacatga
     4561 tacaccaccc tgaaaagggc caagaattaa ctcctctcca tcaaataaat aaaagcctgt
     4621 aaatacagaa tttggaagca tagcatttag catagctgaa gcatttgata agttggcaag
     4681 agcatttgtt tcatctgaga atagagcttg cgcttgtagt aataataatt gataattttc
     4741 gattttctta gacttgttca tagcacttat tatagcataa tttgaaagtt actaaaacta
     4801 aataaccatc ctatataa
//
LOCUS       DATROD010000122         4664 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.124, whole genome shotgun sequence.
ACCESSION   DATROD010000122 DATROD010000000
VERSION     DATROD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.124"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(22..1047)
                     /gene="trpS"
                     /locus_tag="VBC74_001803"
     CDS             complement(22..1047)
                     /gene="trpS"
                     /locus_tag="VBC74_001803"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEO6942722.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQNEGSYTLFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTLFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPAGFLVYPVAQAADITAFKANLVPVGTDQKPMIE
                     QTREIVRSFNHAYNCQVLVEPEGIYPENDAAGRLPGLDGNAKMSKSLNNGIFLADDMD
                     TVKKKVMSMYTDPNHIKVEEPGQIEGNMVFHYLDVFGRDEDQKEITAMKEHYQKGGLG
                     DVKTKRYLLDILERELSPIRERRLEYAKDMGQVYQMLQKGSEKAQAVAASTLDEVKSA
                     MGLNYFK"
     gene            complement(1155..1847)
                     /locus_tag="VBC74_001804"
     CDS             complement(1155..1847)
                     /locus_tag="VBC74_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEO6942723.1"
                     /translation="MRRDDIIMPQDGRKMAVLLGFIGGFIDVYSHMQFHSLVATQTGN
                     IILMVANLGQNDFHFFITKLFSIFFFSIGFLVGIVIKERAVTAFWRLYALCPLLLSTM
                     FIPFVENVNHNIWVALLAFSTGILMLTFSGSRIESEPYTILMTSGNYRKMLQFWYEYI
                     VNRQRTAMQKRRAINYSLVVLAFIIGALVAAIVYDIFAYRAILGVTITLLIIMIHYTI
                     EIIKNDLTLHNV"
     gene            2091..2963
                     /locus_tag="VBC74_001805"
     CDS             2091..2963
                     /locus_tag="VBC74_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEO6942724.1"
                     /translation="MSKKIKNFILLNIGSLIVAFGFNAFFLHNHIVTGGISGLAVSFH
                     EVFGWDSAFFIMIINIPLLLLCYFGLGKQTFLKTVYGSWIFPVFIKLTQSVPTLTHNP
                     LLAALFGGVIVGCGLGIVFWSDSSTGGTGIIIQFLGKYTPISLGQGVILIDGLVTIVG
                     FLAFDSDTVMFSIIGLITISYIINAIQTGFTTLSTVLIVSQEHQKIKTYINTVADRGV
                     TEIPVKGGYSGTNQIMLMTTIAGYEFAKLQEAIAEIDETAFITVTPTSQASGRGFSLQ
                     KNHGRLDEDILMPM"
     gene            3029..4648
                     /locus_tag="VBC74_001806"
     CDS             3029..4648
                     /locus_tag="VBC74_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEO6942725.1"
                     /translation="MLTVSDVSLRFSDRKLFDEVNINFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHIALGPDERLSVLRQNHFDYEDERVIDVVIMGNETLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNISEELHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKESSELAARLQADRNAKAEEKIKQLQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFVNFKAEREMGNDLLTVENLSVTIDGEKI
                     LDNISFILRPGDKTALIGQNDIQTTALIRALMGDIEYEGTIKWGVTTSRSYLPKDNSR
                     DFASGESILEWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKSVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKDFKESIIFASHDHEFIQTLANHIIIL
                     SKNGVIDRIDETYDEFLENTEVQAKVAQLWK"
BASE COUNT         1510 a          821 c          782 g         1551 t
ORIGIN      
        1 ctcttttatt ataggaatat attatttaaa atagttcaaa cccatggctg atttaacctc
       61 atccaaagta gaggctgcaa cagcttgagc tttctcacta cctttttgta acatttgata
      121 aacttgtccc atatctttag catattcaag gcgacgttct ctaataggtg ataattcacg
      181 ctctaagata tcaagtagat aacgttttgt tttaacatct ccaagtccac ctttttgata
      241 gtgttctttc atagcagtaa tctctttttg gtcttcatct cttccaaaaa catcaaggta
      301 atggaatacc atattacctt cgatttgtcc tggttcttct actttaatat ggtttggatc
      361 agtatacatg ctcataactt ttttcttaac tgtatccata tcatctgcta aaaagattcc
      421 attatttaat gacttagaca tttttgcatt accatctaac cctggcagac gacctgctgc
      481 atcattttct ggatagattc cctctggttc aactaaaacc tgacaattat aagcatgatt
      541 gaaagatcta actatttcac gtgtttgttc aatcattggt ttttggtcag tccctacagg
      601 aacaaggtta gctttaaaag cggttatatc agctgcttgt gcaacaggat aaactaagaa
      661 tcctgctgga atagactcac caaaaccttt ttgagcaatt tctgttttta cagttggatt
      721 acgttccaaa cgtgcaagtg aaaccaagtt catataatac atacttaatt ctgctaactc
      781 aggaatttga ctttgaatga ataaagtcga cttgtttgga tccagcccaa cagctaaata
      841 atcaagtgca acattaccaa ttgattcaac aatagtttgc ggatctttag catggtctgt
      901 cagtgcttgt tggtctgcta aaaagacaaa taaagtatag ctaccctcat tttgaagtag
      961 aactctattt tttaagctac ctacataatg tccaatatgc aactttcctg tcggtctatc
     1021 acctgttaag ataattggtt ttgtcatgtt tactccttaa tataccaaag atatcatttc
     1081 atatagttat cttatcatgt cacaagccct attgcaattc atccctacaa gaaacattca
     1141 ccttattaag atgattaaac attatgtaac gttaaatcgt tcttaataat ttcaatcgta
     1201 taatgaatca taataattaa aagcgttatc gtcacaccta aaatagctct atatgcaaag
     1261 atatcgtaaa ctattgcagc taccaatgct ccaataataa atgctagaac taccagtgaa
     1321 taattgatag ctcgtctttt ttgcatagcc gttctctgac gattaacaat atactcatac
     1381 caaaattgta gcattttccg ataattacca gaagtcatta agatagtgta tggttcagat
     1441 tcaatgcgac ttcctgaaaa tgtcaacatt aaaatacctg tgctaaaggc taataaagct
     1501 acccagatat tgtggtttac attttcaaca aaaggaataa acatcgtaga aagtaaaagg
     1561 ggacataatg catatagacg ccaaaaagct gttactgcac gttctttaat cacaatacca
     1621 accaaaaaac caattgaaaa aaagaaaata gaaaataatt tagtgataaa aaagtgaaaa
     1681 tcattttgtc cgagatttgc caccatcaag ataatattac ctgtctgtgt tgcaacaagg
     1741 gagtgaaact gcatgtgact ataaacgtca ataaaaccac caataaaacc caataataca
     1801 gccatttttc ttccatcttg tggcattata atatcatctc tgcgcattat agcaaattct
     1861 ccttcttgta ttatcatgtt ttctattata taccaataaa ataggcattc aactcaaaaa
     1921 tatcgagaaa tttcttttct aaatttactt gactaccaag tctcgctgtt agataatagg
     1981 taataataag ttttgataaa ataaaaatgt tatgactgag ttgtcaatat tatttattga
     2041 cagctttctc tttttatcat gttattatta ctaaagttaa ggagcttatc atgtctaaaa
     2101 aaattaagaa ttttatacta ttaaatattg gatctctaat tgttgcattt ggtttcaacg
     2161 ctttctttct tcataatcat attgtcacgg gggggattag tggtttggca gttagttttc
     2221 atgaagtatt tggttgggat tctgcttttt ttattatgat tatcaatatt ccattgctcc
     2281 ttctttgcta ctttggctta ggtaaacaaa cctttttaaa aactgtctat ggttcttgga
     2341 tttttcctgt ttttattaag ttaacacaaa gtgtaccaac tttgacccac aacccactcc
     2401 tcgcagcact ttttggaggt gttattgtag gatgtggttt ggggattgtt ttttggagcg
     2461 actcttcaac tggtggaacg gggattatca ttcaattctt aggaaaatat actcctataa
     2521 gccttggaca aggggttata ttgattgatg gacttgttac aattgttggt ttcctagctt
     2581 ttgacagtga tacggttatg ttttctatta ttgggttgat aactattagt tatattatta
     2641 atgctatcca aactggattt acaaccttaa gcactgtctt aatcgtttct caagagcacc
     2701 aaaaaattaa gacatatatc aatactgtcg cagatagagg agtaacagaa attcccgtta
     2761 aagggggata ttctggaact aatcaaatca tgcttatgac aactattgct ggttatgagt
     2821 ttgctaaatt acaagaggca atagcagaaa ttgacgaaac agccttcata acagtaactc
     2881 caacatcaca agcttctgga cgtggattta gtcttcaaaa aaatcatgga cgtcttgatg
     2941 aagacattct tatgccaatg taatcgattg ataattaaag ctttttctag tagaatagca
     3001 gatagaatat acacaagagg taacaaaatt gcttacagtt tcagatgttt cactacgttt
     3061 tagtgatcgc aaattatttg atgaagttaa cattaacttt acagcaggaa atacctatgg
     3121 tcttatcgga gcaaatggcg ctggtaaatc aactttcctt aaaattttag ctggtgatat
     3181 tgaaccaaca acgggacata ttgcacttgg cccagatgaa cgcctttctg tacttcgcca
     3241 aaatcatttt gattatgaag atgagcgcgt cattgatgtc gttatcatgg gtaatgaaac
     3301 actttatagt atcatgaaag aaaaggatgc tatctacatg aaagaagatt tttctgatga
     3361 agatggtgtt cgcgctgcgg aattagaagg agaatttgct gaactcggtg gatgggaggc
     3421 tgaaagtgag gcttctcaat tactccaaaa tctaaatatt tcagaagagc ttcattatca
     3481 aaatatgagt gaattagcta atggagacaa agttaaagtt ctccttgcaa aagctctctt
     3541 tggtaaacca gatgtacttc tccttgacga gcctaccaat ggacttgata tccagtctat
     3601 tacttggtta gaagatttct tgattgactt tgaaaataca gttattgtag tttcccacga
     3661 ccgtcatttc ttgaataaag tatgtacaca tatggctgac cttgattttg gaaaaattaa
     3721 actgttcgtc ggcaactatg atttctggaa agaatcatca gaattagcag ctcgtttaca
     3781 agctgatcgt aatgctaaag cagaagaaaa aattaaacaa cttcaagaat tcgttgcacg
     3841 attttcagcg aatgcttcaa aatcaaaaca agcaacttct cgtaaaaaaa tgcttgataa
     3901 aattgaactt gaagagattg taccatcaag ccgtaaatac ccattcgtta actttaaagc
     3961 cgaacgcgaa atgggaaatg atttattgac cgttgaaaat ctctctgtca ctattgatgg
     4021 ggaaaaaatc cttgataata tcagtttcat tcttcgtcct ggtgataaaa ctgcacttat
     4081 tggacaaaat gatatccaaa ctactgcact tattcgagca ttgatggggg atatcgaata
     4141 tgaaggaaca atcaaatggg gagtaacaac tagtcgttct taccttccaa aagacaattc
     4201 acgagacttt gcttctggcg agtcaatctt agaatggctt cgtcaatttg ctagtaaaga
     4261 agaagacgat aataccttct tacgtggctt cctaggtcgt atgcttttct ctggagatga
     4321 ggttaacaaa tctgttaatg tcttatctgg gggtgaaaaa gtacgcgtta tgctttcgaa
     4381 attaatgcta ttaaaatcta atgttcttgt tcttgatgat ccaacaaacc acttagattt
     4441 ggaatctatt tcaagtctca atgatggact aaaagatttc aaagaatcta ttatttttgc
     4501 aagtcatgac cacgagttta ttcaaacttt agcgaaccat attattattc tttctaaaaa
     4561 cggtgttatc gatcgtatcg atgaaactta cgatgaattt cttgaaaata ccgaagtaca
     4621 agctaaggtc gctcaactct ggaaataatc ctttggcata tctc
//
LOCUS       DATROD010000123         4652 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.125, whole genome shotgun sequence.
ACCESSION   DATROD010000123 DATROD010000000
VERSION     DATROD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.125"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..485
                     /locus_tag="VBC74_001807"
     CDS             <1..485
                     /locus_tag="VBC74_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942726.1"
                     /translation="ELTDFALDAVKKYNSDKLELVFDTVESLICDELEDQEFQKFKKK
                     VLNNFKYIKPAHLRNLSNRGIGIMESQHRKITYRMKRRGMYWSKWGISTMANMIILER
                     ANGLRELFFGSWRKVYSEYKEGSFSAGRLFKKTDELDKFSKPLLKNGRKWSITGIKTK
                     "
     gene            1139..4591
                     /gene="scpA/B"
                     /locus_tag="VBC74_001808"
     CDS             1139..4591
                     /gene="scpA/B"
                     /locus_tag="VBC74_001808"
                     /EC_number="3.4.21.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C5a peptidase ScpA/B"
                     /protein_id="HEO6942727.1"
                     /translation="MRKKQKLPFDKLAIALISTSILLNAQSDIKANTVTEDTPATEQA
                     VEPPQPIAVSEESRSSKETKTSQTPSDVGETVADDANDLAPQAPAKTADTPATSKATI
                     RDLNDPSHVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKENLEKAK
                     KEHGITYGEWVNDKVAYYHDYSKDGKNAVDQEHGTHVSGILSGNAPSEMKEPYRLEGA
                     MPEAQLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDETK
                     KAFDYAKSKGVSIVTSAGNDSSFGGKPRLPLADHPDYGVVGTPAAADSTLTVASYSPD
                     KQLTETATVKTDDHQDKEMPVISTNRFEPNKAYDYAYANRGTKEDDFKDVEGKIALIE
                     RGDIDFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRRDGLLLKDNP
                     PKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAKL
                     SGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYFS
                     PRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELYY
                     QVTVQTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMKN
                     GYFLEGFVRFKQDPTKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSDA
                     KDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGTF
                     AKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGNV
                     VWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPISS
                     GAKEQHTDFDVIVDNTTPEVATSATFSTEDSRLTLASKPKTSQPVYRERIAYTYMDED
                     LPTTEYISPNEDGTFTLPEEAETMEGATVPLKMSDFTYVVEDMAGNITYTPVTKLLEG
                     HSNKPEQDGSDQAPDKKPEAKPEQDGSGQTPDKKKETKPEKDSSGQTPGKTPQKGQSS
                     RTLEKRSSKRALATKASTRDQLPTTNDKDTNRLHLLKLVMTTFFLGLAAHIFKTKRQK
                     ETKK"
BASE COUNT         1636 a          903 c          911 g         1202 t
ORIGIN      
        1 ttgaattaac cgattttgct ttagatgcgg taaaaaaata taattctgat aagcttgaat
       61 tagtttttga tactgttgaa tcactgattt gtgatgaact tgaagatcaa gaatttcaga
      121 agtttaagaa aaaagtatta aataatttca aatatataaa accagctcat cttagaaatc
      181 tttcaaatcg tggtattggt atcatggaat cacaacacag aaagataacg tatagaatga
      241 agcgacgtgg catgtattgg tcaaagtggg gaatctccac aatggcaaat atgattatac
      301 ttgaaagagc taacggttta cgagaattat ttttcggttc ttggagaaag gtatacagtg
      361 agtataaaga aggttcattt agtgcagggc gactttttaa aaagacagat gaattagata
      421 aattttctaa gccccttcta aaaaatggca gaaaatggag tataacagga atcaaaacaa
      481 aatagaaaaa gtctaaagat acctttattt ctagcgtttt tttgcatgtt ttaacttgca
      541 ttaaatcttc taaagtagta taattatatt aaagaaaagc aaaaaaggtg aaggagcgcg
      601 aactcctaca ccaaaccgat taagacaaat accacttgct taatcagtcg aaccttattg
      661 tagcataact aaaacaatac cgtcaagact gaacaagcaa aaaatacttg ttttggtctt
      721 tttaagaata attcaagttt tcttaaattt atgaggtatc gactaatctc aaaatttcag
      781 acacatacct gagtgaacat caagagagaa ccgatcggtt ctctcttgat gtctaaaaga
      841 atgaggttaa ggaggtcaca aactaaacaa ctcttaaaaa gatgaccttt actaataatc
      901 gtctttgttt tataataaag atgttagtaa tataattgat aaatgagata catttaatca
      961 ttatggcaaa agcaagaaaa atagctgtgt catatgcaaa taacccctgt ttgctcttta
     1021 aaaaagacgt tatccttatt tctctacaca cagacggata gctaggagag aatcgtttga
     1081 ttctctcttt tcttaatggt cataaagaca aagtctctca tcatgaaagg acgacacatt
     1141 gcgtaaaaaa caaaaactac catttgataa acttgccatt gcgcttatat ctacgagcat
     1201 cttgctcaat gcacaatcag acattaaagc aaatactgtg acagaagaca ctcctgctac
     1261 cgaacaagcc gtagaacccc cacaaccaat agcagtttct gaggaatcac gatcatcaaa
     1321 ggaaactaaa acctcacaaa ctcctagtga tgtaggagaa acagtagcag atgacgctaa
     1381 tgatctagcc cctcaagctc ctgctaaaac tgctgataca ccagcaacct caaaagcgac
     1441 tattagggat ttgaacgacc cttctcatgt caaaaccctg caggaaaaag caggcaaggg
     1501 agctgggacc gttgttgcag tgattgatgc tggttttgat aaaaatcatg aagcgtggcg
     1561 cttaacagac aaaactaaag cacgttacca atcaaaagaa aatcttgaaa aagctaaaaa
     1621 agagcacggt attacctatg gcgagtgggt caatgataag gttgcttatt accacgacta
     1681 tagtaaagat ggtaaaaacg ctgttgatca agaacacggc acacacgtgt cagggatctt
     1741 gtcaggaaat gctccatctg aaatgaaaga accttaccgc ctagaaggtg cgatgcctga
     1801 ggctcaattg cttttgatgc gtgtcgaaat tgtaaatgga ctagcagact atgctcgtaa
     1861 ctacgctcaa gctatcagag atgctgtcaa cttgggagct aaggtgatta atatgagctt
     1921 tggtaatgct gcactagctt acgccaacct tccagacgaa accaaaaaag cctttgacta
     1981 tgccaaatca aaaggtgtta gcattgtgac ctcagctggt aatgatagta gctttggggg
     2041 caagccccgt ctacctctag cagatcatcc tgattatggg gtggttggga cacctgcagc
     2101 ggcagattca acattgacag ttgcttctta cagcccagat aaacagctca ctgaaactgc
     2161 tacggtcaaa acagacgatc atcaagataa agaaatgcct gttatttcaa caaaccgttt
     2221 tgagccaaac aaggcttacg actatgctta tgctaatcgt ggtacgaaag aggatgattt
     2281 taaggatgtc gaaggtaaga ttgcccttat tgaacgtggc gatattgatt tcaaagataa
     2341 gattgcaaac gctaaaaaag ctggtgctgt aggggtcttg atctatgaca atcaagacaa
     2401 gggcttcccg attgaattgc caaatgttga ccagatgcct gcggccttta tcagtcgaag
     2461 agacggtctc ttattaaaag acaatccccc aaaaaccatt accttcaatg cgacacctaa
     2521 ggtattgcca acagcaagtg gcaccaaact aagccgcttc tcaagctggg gtctgacagc
     2581 tgacggcaat attaagccgg atattgcagc acccggccaa gatattttgt catcagtggc
     2641 taacaacaag tatgccaaac tttctggaac tagtatgtct gcaccattgg tagcgggtat
     2701 catgggactg ttgcaaaagc aatatgagac acagtatcct gatatgacac catcagagcg
     2761 tcttgattta gctaagaaag tattgatgag ctcagcaact gccctatatg atgaagatga
     2821 aaaagcttat ttttctcctc gccaacaggg agcaggagca gtcgatgcta aaaaagcttc
     2881 agcagcaacg atgtatgtaa cagataagga caatacctca agcaaggttc acctgaacaa
     2941 tgtttctgat aaatttgaag taacagtaac agttcacaac aaatctgata aacctcaaga
     3001 gttgtattac caagtaactg ttcaaacaga taaagtagat ggaaaacact ttgccttggc
     3061 tcctaaagca ttgtatgaga catcatggca aaaaatcaca attccagcca atagcagcaa
     3121 acaagtcacc gttccaatcg atgctagtcg atttagcaag gacttgcttg cccaaatgaa
     3181 aaatggctat ttcttagaag gttttgttcg tttcaaacaa gatcctacaa aagaagagct
     3241 tatgagcatt ccatatattg gtttccgagg tgattttggc aatctgtcag ccttagaaaa
     3301 accaatctat gatagcaaag acggtagcag ctactatcat gaagcaaata gtgatgccaa
     3361 agaccaatta gatggtgatg gattacagtt ttacgctctg aaaaataact ttacagcact
     3421 taccacagag tctaacccat ggacgattat taaagctgtc aaagaagggg ttgaaaacat
     3481 agaggatatc gaatcttcag agatcacaga aaccattttt gcaggtactt ttgcaaaaca
     3541 agacgatgat agccactact atatccaccg tcacgctaat ggcaaaccat atgctgcgat
     3601 ctctccaaat ggggacggta acagagatta tgtccaattc caaggtactt tcttgcgtaa
     3661 tgctaaaaac cttgtggctg aagtcttgga caaagaagga aatgttgttt ggacaagtga
     3721 ggtaaccgag caagttgtta aaaactacaa caatgacttg gcaagcacac ttggttcaac
     3781 ccgttttgaa aaaacgcgtt gggacggtaa agataaagac ggcaaagttg ttgctaacgg
     3841 aacctacacc tatcgtgttc gctacacgcc gattagctca ggtgcaaaag aacaacacac
     3901 tgattttgat gtgattgtag acaatacgac acctgaagtc gcaacatcgg caacattctc
     3961 aacagaagat agtcgtttga cacttgcatc taaaccaaaa accagccaac cggtttaccg
     4021 tgagcgtatt gcttacactt atatggatga ggatctgcca acaacagagt atatttctcc
     4081 aaatgaagat ggtaccttta ctcttcctga agaggctgaa acaatggaag gcgctactgt
     4141 tccattgaaa atgtcagact ttacttatgt tgttgaagat atggctggta acatcactta
     4201 tacaccagtg actaagctat tggagggcca ctctaataag ccagaacaag acggttcaga
     4261 tcaagcacca gacaaaaaac cagaagctaa accagaacaa gacggttcag gtcaaacacc
     4321 agataaaaaa aaagaaacta aaccagaaaa agatagttca ggtcaaacac caggtaaaac
     4381 tcctcaaaaa ggtcaatctt ctcgtactct agagaaacga tcttctaagc gtgctttagc
     4441 tacaaaagca tcaacaagag atcagttacc aacgactaat gacaaggata caaatcgttt
     4501 acatctcctt aagttagtta tgaccacttt cttcttggga ttagcagctc atatatttaa
     4561 aacaaaacgc caaaaagaaa ctaaaaaata gtattactta aactgataag agcttattta
     4621 atagagagaa ccaatttggt tctctctatt tt
//
LOCUS       DATROD010000124         4562 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.126, whole genome shotgun sequence.
ACCESSION   DATROD010000124 DATROD010000000
VERSION     DATROD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4562
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.126"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            5..78
                     /locus_tag="VBC74_001809"
     tRNA            5..78
                     /locus_tag="VBC74_001809"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Asn,seq:gtt)
     gene            218..1068
                     /locus_tag="VBC74_001810"
                     /pseudo
     CDS             218..1068
                     /locus_tag="VBC74_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688173.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
     gene            1155..1598
                     /locus_tag="VBC74_001811"
     CDS             1155..1598
                     /locus_tag="VBC74_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEO6942728.1"
                     /translation="MTVLEQKLDHLVSQILLKAENQHELLFGTCQSDVKLTNTQEHIL
                     MLLSQEQLTNSDLAKKLNISQAAVTKAVKSLISQDMLKANKDSKDARITYFELSELAK
                     PIADEHTHHHDNTLGVYGRLVNHFSKDEKVVLERFLDLFSRELEG"
     gene            1601..2311
                     /locus_tag="VBC74_001812"
     CDS             1601..2311
                     /locus_tag="VBC74_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEO6942729.1"
                     /translation="MRYITVSGLTFQYDSDPVLEGVNYHLDSGEFVTLTGENGAAKST
                     LIKATLGILTPKVGTVNISKENKEGKKLRIAYLPQQIASFNAGFPSSVYEFVKSGRYP
                     RNGWFRRLTKHDEEHIRVSLEAVGMWDNRHKKIGSLSGGQKQRAVIARMFASDPDIFV
                     LDEPTTGMDAGTTEKFYELMHHNAHKHGKSVLMITHDPDEVKGYADRNIHLVRNQSLP
                     WRCFNVHTNEMEVESDAT"
     gene            2301..3113
                     /locus_tag="VBC74_001813"
     CDS             2301..3113
                     /locus_tag="VBC74_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEO6942730.1"
                     /translation="MLLDMLSYDFMQRALLAVVAISIFAPILGIFLILRRQSLMSDTL
                     SHVSLAGVALGVVLGISPTWSTIFVVTLAAVVLEYLRTVYKHYMEISTAILMSMGLAI
                     SLIVMSKAHNVGNVSLEQYLFGSIITIGKEQVIALFVIALITFILTILFIRPMYILMF
                     DEDTAFVDGLPVRTMSILFNVVTGIAIALTIPAAGALLVSTIMVLPASIAMRLGRNFK
                     TVIFLGMLIGFVGMVAGIFLSYYWETPASATITMIFIGIFLLVSLVGLLRKR"
     gene            complement(3324..4354)
                     /locus_tag="VBC74_001814"
                     /pseudo
     CDS             complement(3324..4354)
                     /locus_tag="VBC74_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767962.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
BASE COUNT         1326 a          659 c          892 g         1685 t
ORIGIN      
        1 ataatccgcc atagctcagt tggtagtagc gcatgactgt taatcatgat gtcgtaggtt
       61 cgagtcctac tggcggagta gataaaggag actaactttg gttagccttt ttttattgga
      121 attattattc cttaaggtcg gctagcatct taattgtccg aatttgtcat ttgttatgtt
      181 ataatatgga tattctatat tttattaggg aggttttatg aaaatttttg agaaagctcc
      241 tgccaaactt aatttaggat tagatattaa aggacgatgt gacgatggtt atcatgaatt
      301 agctatgatt atggttagta ttgatcttaa tgactacgtt accatttctg agctaaagga
      361 agattgtatt gttatcgatt ctgatagtag caaaatgcct ttaaataatg ataatgatgt
      421 gtttaaagct gcagatataa tcaaaaatca atatggtatt aataaaggtg ttcatattag
      481 attagaaaaa tccattccta tctgtgcagg actggggggg ggtctactga tgctgctgct
      541 acaataagag ctcttaatcg attatggaat cttcaaatgg gccatgacga aatggttgct
      601 attggtttta aaattggtag tgatgttcct tattgccttg gtgggggatg ttcgttagta
      661 ttaggtaaag gtgaaattgt taaaccctta ccgacattga gaccatgttg gatagttctg
      721 gttaagccgg attttggtat ttcaactaaa tccattttta gagatattga ttgtaagtct
      781 ataagcagag ttgatattga tttattaaag tctgctattt tgtcatcaga ttatcagtta
      841 atggtcaaat ctatgggaaa ttcactcgag gatattacaa ttactaaaaa tccagttata
      901 agtacaataa aagaacgtat gttaaattct ggagccgatg ttgcattaat gacaggaagt
      961 ggaccaactg ttttttcaat gtgttctaca gagaaaaaag ctgatcgtgt ttttaatagt
     1021 atgaaaggtt tttgcaaaga agtttataaa gttaggttgt tgagataaaa ggttttatct
     1081 tcttcaccta tctatgattg attcttctat atcttcgtga tataatttac tggttaacaa
     1141 aaaaagaggt atatatgaca gttttagaac aaaaattaga ccatttagtg agtcaaatct
     1201 tgcttaaggc agaaaatcaa catgaattat tgtttggtac ttgtcaaagt gatgtaaaat
     1261 tgactaatac gcaagaacac attctgatgc ttttatcaca agagcagttg acaaattctg
     1321 atttagctaa gaagttaaat attagtcagg cagctgtaac taaagcagta aaaagtttaa
     1381 tttctcagga tatgttgaaa gcgaataaag attctaaaga tgctcgtatt acttattttg
     1441 agttatctga acttgctaaa cccattgcag atgaacacac acatcatcat gacaatacgt
     1501 taggggttta cggaagatta gtaaatcatt tttcaaagga cgaaaaggtc gttttagaac
     1561 gttttcttga tcttttctct agagaattag aagggtaatc atgcgatata ttacagttag
     1621 tggtcttact tttcagtatg atagcgatcc tgtgctggaa ggggttaatt accatttaga
     1681 cagtggggag tttgttacct taacaggtga aaatggggct gctaaatcaa ctttaataaa
     1741 ggctacttta ggtattttaa cgcccaaagt gggtacagtt aatatctcta aagaaaacaa
     1801 agaaggaaaa aaattacgta ttgcctattt acctcaacag attgcaagtt ttaacgctgg
     1861 ctttccatcg tcagtttatg aatttgtgaa atctggacga taccctagaa atggttggtt
     1921 tagaagactg actaaacatg atgaagaaca tattagggtt agtttagaag ctgttggtat
     1981 gtgggataat cgccataaaa agataggaag tttatctggc ggtcaaaagc agagggctgt
     2041 cattgctagg atgtttgctt ctgatccaga tatttttgta ttggatgagc caacaactgg
     2101 tatggacgca gggactactg agaaatttta tgagttaatg catcataatg ctcataagca
     2161 tggtaagtct gttttgatga ttactcacga tcctgatgaa gtaaaggggt atgctgaccg
     2221 aaatattcat cttgttagga accaatcttt accatggcgt tgtttcaatg tccatacaaa
     2281 cgaaatggag gttgaatcag atgctacttg atatgctttc ctatgatttt atgcaacgag
     2341 cgctcttagc agttgtggct attagtattt ttgctccaat tttaggcatc ttcctaattt
     2401 tacgtcgtca gagtttgatg agtgatactt tgagccacgt ttccttagcc ggtgttgcat
     2461 taggcgttgt tttaggtatc tcgcctacgt ggtcaactat tttcgttgtt actttggcgg
     2521 cagttgtatt agagtatttg cgtactgtat acaaacatta tatggaaata tcaactgcta
     2581 tcctgatgtc gatggggttg gctatatctt taatcgttat gagtaaagct cataatgttg
     2641 gtaatgtcag tcttgaacag tatttatttg gttctattat cactattggg aaggaacaag
     2701 tgattgcttt gtttgttatt gcattaatca cttttatttt aaccatccta tttattagac
     2761 caatgtatat attaatgttt gatgaggata cagcttttgt agatggattg cctgtgcgta
     2821 caatgtctat tttgttcaat gttgttacag gtattgctat tgcgttaaca attcctgccg
     2881 caggtgctct attggtgtca actattatgg ttttaccagc tagtattgct atgcggttag
     2941 gtcgaaattt taaaactgtt atttttcttg ggatgttaat tggatttgtg ggaatggtag
     3001 ctggtatttt cctttcttat tattgggaga cacctgctag tgcaacaata acgatgattt
     3061 ttattggcat tttcctatta gttagcttag taggcttgct tcgcaaacgt taaataaaaa
     3121 aatctatctt attctaggat agatttttta tttatctccc tgtttttttc gaatatattt
     3181 tatgagggat cagttgagaa attatctcga tgaggtatct actaagtttt ctggcttaag
     3241 ttgatagaag gggcaatgtg aatctatttt tgcgttttag gataaaaaat actaccggca
     3301 attttgccgg cagtaaattg taattattgt gcttcagtag tgctgtgata tttgtgttga
     3361 tttaacgctt cttgttcagt catttgaacg atattagcgg tatttgcatt ttttatgttt
     3421 tctaaactat accaataagt atttgaacga cccttatttg caacgtagac gattctattt
     3481 ttgggagcta cattatttgt atctgattca gaagtattag tgttagcatt ttcttctata
     3541 tcagatgcac cttcaatagc tgcttcgttt tcatcagcat cgctatttgg atcttcacct
     3601 tcttctgcct gtttcccatc ttctttgatt agcataccag ttttgtaatc aatttttgcg
     3661 agtggagacg tattttctaa tgtaacagtt gtgacgccgt attcatcaac ttgttctttg
     3721 ccgccgccta atttgatttc tagaagtttt ccttgtttat caattccaac atactgaagt
     3781 tcaactcggc taggtactaa attatttcct tcgtatattg gagtcacttt gtaatcaagc
     3841 tagaaatttt tatgatttgc caaccaacta tctaatctat tttcgtagta taacatacca
     3901 tcaggattat tttcgctaaa tcctgtattt agatatttgg tcatagtaac taggttctca
     3961 ggaacattgt tcattcctga aaattgataa ccaacgaggt gacctcggtc tataagccaa
     4021 gttgttttac ccttttcagt agtaaatttg tagttgtgcc aaccggttgg attatacgtc
     4081 agtctttctc tttttatctt aggctcatct gcatccctta attgtatgtg agaatatgtt
     4141 gctctttcta agttatctaa tgaacctacc ttgatttgct tattatgagt aaacggtaag
     4201 atttttttga agaagtactt acatactgtg tctcaacatg ggatgtcgtc ttataatgtt
     4261 tagccttagc ttcaacattc tgttgtaatg ccccaacgca tagtacgaca gttaatagtg
     4321 ggagtgattt gtataattgt ttagataatt tcataaacct aaacccttct aaatgatatt
     4381 tgtgaaggcc tttcacaagt ataattttat cattttattg ggaaaaagta ataaaaaaac
     4441 ataactagtt aatttttttg gagattatgt tatttaacta gatagttatt aaagttaaat
     4501 gttatagata tactgttatt acataataat ttttatatga tttgttattt tattagattt
     4561 tt
//
LOCUS       DATROD010000125         4451 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.127, whole genome shotgun sequence.
ACCESSION   DATROD010000125 DATROD010000000
VERSION     DATROD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.127"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..2158
                     /gene="nrdE"
                     /locus_tag="VBC74_001815"
     CDS             <1..2158
                     /gene="nrdE"
                     /locus_tag="VBC74_001815"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEO6942731.1"
                     /translation="SLKNIGDVSYFRLNNEINRPVNGQIPLHKDKEALTAFFKENVQP
                     NSKAFDSITDKIAYLLKYDYLEEAFLNKYRPEFIEELSTKLFDKKFRFKSFMAAYKFY
                     QQYALKTNDGEYYLESIEDRVLFNALYFADGDEELATDLALEMISQRYQPATPSFLNA
                     GRSRRGELVSCFLIQVTDDMNAIGRSINSALQLSRIGGGVGISLSNLREAGAPIKGFA
                     GAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLDVFHPDIISFLSTKKENADEKVRV
                     KTLSLGITVPDKFYELARNNQEMYLFSPYSIEREYGVPFSYIDITEKYDELVANPNIT
                     KTKINARDLETEISKLQQESGYPYIINIDTANRTNPVDGKIIMSNLCSEILQVQKPSL
                     INDAQEYLEMGTDISCNLGSTNVLNMMTSPDFGKSIKTMTRALTFVTDSSNIEAVPTI
                     KNGNAQAHTFGLGAMGLHSYLAKNHIEYGSPESIEFTDIYFMLMNYWTLVESNNIARE
                     RQTTFVGFEKSKYADGTYFDKYVSGKFVPQSDKVKSLFTNHFIPEAKDWENLRYAVMK
                     DGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLATDTIP
                     YYTSAYDMDMRKVIDVYAAATEHVDQGLSMTLFLRSELPKELYEWKTESKQTTRDLSI
                     LRNYAFNKGVKSIYYIRTFTDDSSEVGANQCESCVI"
     gene            2361..3320
                     /gene="nrdF"
                     /locus_tag="VBC74_001816"
     CDS             2361..3320
                     /gene="nrdF"
                     /locus_tag="VBC74_001816"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEO6942732.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSAQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKSEIEEIFEWTNNNEFLQEKARIINDIYANGNALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMHDDDYNYGL"
     regulatory      3405..3503
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3560..4123
                     /gene="thiT"
                     /locus_tag="VBC74_001817"
     CDS             3560..4123
                     /gene="thiT"
                     /locus_tag="VBC74_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEO6942733.1"
                     /translation="MSKNNNTTCLIETAIFAALAMALSMIPDFASWFTPSFGAIPLIL
                     FALRRGTKYGLFAGLIWGLLHFVLSKVYYLSLSQVFIEYILAFISMGLAGVFSAKFKD
                     ALSSSSKTKALSLALSGAILATFVRYVWHYIAGVIFWASYAPKGMSATLYSLSVNGTA
                     GLLTLFFVVISIIILVISYPSFFLPKK"
     gene            complement(4208..>4451)
                     /locus_tag="VBC74_001818"
     CDS             complement(4208..>4451)
                     /locus_tag="VBC74_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEO6942734.1"
                     /translation="LKELKNNVLQLDEAELESNYKMCFDIVMNLSGFIFPTIYFVLFF
                     ISFVFQKVEIVAIIITTSIHIYILIKSLKAARHFYR"
BASE COUNT         1472 a          797 c          731 g         1451 t
ORIGIN      
        1 gagtttaaaa aatattggcg atgtatctta ctttcgccta aataatgaaa ttaaccgtcc
       61 tgttaatgga caaatcccac tccataaaga taaagaagcc ttaactgctt ttttcaaaga
      121 aaacgtacag cctaattcaa aagcttttga ctcaatcact gataaaattg cttacctcct
      181 taagtatgat tatcttgaag aagctttcct caataaatat cgtcctgaat ttattgaaga
      241 attatctacc aaactcttcg ataaaaagtt tcgttttaaa tccttcatgg cagcctataa
      301 gttttatcag cagtatgcat taaaaacaaa tgatggagaa tactatttag aaagtattga
      361 agatcgcgtt ctctttaacg ctctttattt tgcagacggt gatgaagaac tagcaactga
      421 cttagctcta gaaatgattt cacaacgcta tcaacctgca acaccaagtt ttttaaatgc
      481 tggtcgaagt cgtcgcggag aattagtctc atgtttctta atccaagtaa ctgatgatat
      541 gaatgctatt ggtcgttcga ttaattcagc tcttcaatta tctcgtattg gtggtggggt
      601 tggaatatca cttagtaacc ttcgtgaggc tggagctcct atcaaaggtt ttgctggtgc
      661 tgcttctgga gtcgttccag tcatgaaatt atttgaagac agtttttctt actccaacca
      721 acttggacaa cgccaaggtg ctggagttgt ttaccttgac gttttccatc cagatattat
      781 ttcattccta tcaactaaaa aagaaaatgc tgatgaaaaa gtacgtgtta aaacattgtc
      841 attagggatt actgtacccg ataaattcta tgaactagca cgtaacaatc aagaaatgta
      901 cttgtttagt ccttatagta tcgaacgtga atatggtgtt cctttcagct atattgatat
      961 taccgaaaaa tatgatgaat tagtcgctaa ccctaatatc actaaaacaa aaatcaatgc
     1021 tcgtgattta gaaacagaga tttctaaact tcaacaagaa tctggttatc cttacatcat
     1081 caatattgac actgctaatc gtactaatcc tgttgacgga aaaattatta tgtcaaatct
     1141 ttgctctgag attttgcaag tacaaaaacc aagtcttatc aatgatgctc aagaatacct
     1201 tgaaatggga actgatattt cttgtaacct tggctcaact aatgttttaa atatgatgac
     1261 atctcctgat tttggaaaat caattaaaac aatgactcgt gctctaactt tcgttactga
     1321 ctcatcaaat attgaagctg ttccaactat taagaacgga aatgctcaag cacatacatt
     1381 tggacttgga gcaatgggac tccactccta tttggcaaaa aatcatatcg aatatggtag
     1441 tccagaatca attgaattta ctgatattta tttcatgcta atgaactact ggaccttagt
     1501 agaatcaaac aatattgccc gcgaacgtca aacaacattt gtaggttttg aaaaatcaaa
     1561 atacgctgac ggtacatact ttgataaata tgtatctgga aaatttgttc cacaatctga
     1621 caaagtaaaa tccctattta ctaatcattt tatccccgaa gctaaagatt gggaaaacct
     1681 tagatacgca gttatgaaag acggactata tcatcaaaat cggcttgcag ttgctccgaa
     1741 tggttctatt tcgtatatca acgattgttc agcatctatc caccctatta cacaacgtat
     1801 tgaagagcgt caagagaaaa aaatcgggaa aatttattat ccagctaatg gcttagctac
     1861 agatactatc ccttattaca cttctgccta tgatatggat atgcgtaaag taatagacgt
     1921 ctatgccgca gcaactgagc acgtagacca aggtctctca atgacactct ttttacgaag
     1981 cgaattacca aaggagttgt acgagtggaa aacagaaagt aagcagacta ctcgcgacct
     2041 ttcaatccta cgtaactatg ctttcaacaa aggtgtgaaa tcaatctact atatccgtac
     2101 ttttacagat gatagtagcg aagtcggagc taatcaatgc gaaagttgtg tcatctaacg
     2161 aagaaactct acttaaaaca tcagaaacta gactgagata aactatctca gtttcatgat
     2221 gttatcttat tttctaaggt attttcatct ttatttttct aaaaatcgat aatacttgaa
     2281 atattgctag atcagaattc tatattctac tcttcttcca tcatcattat ataaaaacct
     2341 atttaccatt ggagaaaacc atgacaactt actacgaagc tataaactgg aacgaaattg
     2401 aagatgttat tgataaatca acttgggaaa aactaaccga acaattttgg ctcgatacac
     2461 gtatcccttt atcaaatgac ttagacgatt ggcgcaaact ttccgctcaa gaaaaagatc
     2521 ttgttggcaa ggtttttgga ggcttaaccc tacttgatac catgcaatca gaaactggtg
     2581 ttgaagctat tcgtgccgat gttcgcacgc ctcacgaaga agctgtctta aacaatattc
     2641 aattcatgga atctgttcac gctaaatctt attcttcaat tttctcaact ttaaatacta
     2701 aatcagaaat tgaagaaatt ttcgagtgga ctaataataa tgagttcctt caagaaaaag
     2761 cacgtattat caatgacatt tatgctaatg gaaatgccct tcaaaaaaag gtggcttcca
     2821 cctacctcga aactttcctt ttttattctg gctttttcac acctctttac tatttgggaa
     2881 ataataagtt agcaaatgtt gctgaaatca ttaaattaat tattcgtgat gaatctgtac
     2941 atggtactta tatcggttac aaattccagc ttggttttaa cgaattacca gaagatgagc
     3001 aagagaattt tcgtgattgg atgtatgacc tcctttatca gctgtatgaa aacgaagaaa
     3061 aatacaccaa gacactttat gatggcgtag gatggactga agaagttatg acctttttac
     3121 gctacaatgc taataaagct cttatgaatt taggacaaga tcctttattc ccagatacag
     3181 caaatgatgt caacccaatt gttatgaatg gtatttcaac aggaacatca aaccatgact
     3241 tcttctctca agtaggtaat ggttacctac ttggtagcgt tgaagctatg catgatgatg
     3301 actataacta tggattataa gaaaaagaca ctgatttagt gtctttttct agttaaatac
     3361 aaatttcttg acaagcaaac cctttcaaga tagaatgtta gagtaaatac acaagggagt
     3421 gccttgagct gagattgcag atatgcaaaa tcctctaacc tgatctcgtt aggacgagcg
     3481 taggaattgt ggtttgaatt ggttaaacat gacaaatgac aaaagaatct cctttgctgt
     3541 ataaaaaagg agatttttta tgtcaaaaaa taacaacact acctgtctta ttgaaactgc
     3601 tatttttgca gcgcttgcta tggctttatc aatgattcct gattttgcgt catggttcac
     3661 tccctctttt ggagcaattc ctctgatact ttttgcatta cgtcgtggta caaaatacgg
     3721 tctatttgct ggcttaattt ggggcttatt gcactttgtt ctttccaaag tatattacct
     3781 tagtttatcc caagttttta tagaatacat cttagcattt ataagtatgg gacttgcagg
     3841 cgtattttct gctaaattta aagatgcact tagcagcagc tctaaaacta aagctttatc
     3901 attagcatta tctggagcaa tattagcaac atttgttcgt tatgtttggc attatatcgc
     3961 tggtgttatt ttttgggcta gttatgcacc aaaaggtatg tctgctacat tatactcatt
     4021 aagcgtcaat ggtactgctg gtttgttaac tctatttttt gtagtcatta gtattattat
     4081 tttagttatc agctacccta gcttcttttt acctaagaaa tagaaaaaat aaaaggactg
     4141 attttcagag aatcaatcct tttattttac actaatataa gttttttgat aaaaaatcta
     4201 gctactacta tctataaaaa tgacgtgctg cttttaaaga cttaatgaga atgtagatat
     4261 gaatagatgt tgttataata attgctacaa tctccacttt ttgaaaaaca aatgagataa
     4321 agaataatac aaaatagatc gtagggaaaa taaaaccact taaattcatg acaatatcaa
     4381 aacacatctt ataatttgat tccaactctg cttcgtccaa ttgtaagaca ttgtttttca
     4441 actcttttaa a
//
LOCUS       DATROD010000126         4430 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.128, whole genome shotgun sequence.
ACCESSION   DATROD010000126 DATROD010000000
VERSION     DATROD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.128"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            125..892
                     /gene="cfb"
                     /locus_tag="VBC74_001819"
     CDS             125..892
                     /gene="cfb"
                     /locus_tag="VBC74_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfb"
                     /protein_id="HEO6942735.1"
                     /translation="MNVKHMMYLSGTLVAGALLFSPAVLEVHADQVTTPQVVNHVNSN
                     NQAQQMAQKLDQDSIQLRNIKDNVQGTDYEKPVNEAITSVEKLKTSLRANPETVYDLN
                     SIGSRVEALTDVIEAITFSTQHLTNKVSQANIDMGFGITKLVIRILDPFASVDSIKAQ
                     VNDVKALEQKVLTYPDLKPTDRATIYTKSKLDKEIWNTRFTRDKKVLNVKEFKVYNTL
                     NKAITHAVGVQLNPNVTVQQVDQEIVTLQAALQTALK"
     gene            1065..1367
                     /locus_tag="VBC74_001820"
     CDS             1065..1367
                     /locus_tag="VBC74_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEO6942736.1"
                     /translation="MTKKITVVALETLIAQHNNIHLIDVREEHEYRGGHIPGAINLPL
                     SQLSHKFEQLDKNKEYYLVCQRGGRSIRACQFLELKGYKVINVDGGTEAWRGNLEV"
     gene            complement(1524..2387)
                     /locus_tag="VBC74_001821"
     CDS             complement(1524..2387)
                     /locus_tag="VBC74_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6942737.1"
                     /translation="MNQEKKRLNLIGLILLAQAIVLSLATTLFAEILQNDVWIGIAST
                     LIALLIPCFFMGFKNLKKHLTATHHTITFTTILFLLSIIYTTNNIVSTIWDAVLNHLN
                     IMESESQNYDATFSILMISYSVVVGPFFEEVLYRGIVLNLLSKYGKWFAIITSGILFG
                     LMHQDISQLLTTSIAGIIMGFIAYHYSFKVALLLHICNNFIVEIFTQLSTVNELYGTY
                     FENILLILAILFILYYLFTHRNTARRRISLHSNVREADSINSKQHTKLLLTSWPFILL
                     VIYDIVLTTIN"
     gene            complement(2692..3252)
                     /locus_tag="VBC74_001822"
     CDS             complement(2692..3252)
                     /locus_tag="VBC74_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEO6942738.1"
                     /translation="MRITIFCGASTGENPVYSEKTVALAQWMAQNKHALVYGGGKVGL
                     MGVMADTVIANGGYTTGVIPTFLRDREIAHENLSELIIVNNMPERKAKMMLLGDAFIA
                     LPGGPGTLEEISEVISWSRIGQNDNPCILYNVNGYFNDLKNMFDHMVSEGFLSLEDRE
                     NVLFSDDIAEIEDFITNYKVPSTRQY"
     gene            complement(3249..3566)
                     /locus_tag="VBC74_001823"
     CDS             complement(3249..3566)
                     /locus_tag="VBC74_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEO6942739.1"
                     /translation="MDITDYQKWVSEFYKKRNWYQYNSFIRSNFLSEEVGELAQAIRK
                     YEIGRDRPDETEQTDLENLNDIKEELGDVLDNIFILADQYNISLEEIISAHRTKLEKR
                     FET"
     gene            3780..4394
                     /locus_tag="VBC74_001824"
     CDS             3780..4394
                     /locus_tag="VBC74_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEO6942740.1"
                     /translation="MNKKSFFQCVILLVSFLVLTLAVKSQSDMISYLDNITSAFFQSI
                     RNSDLTNLMTIISTVVSPLTTSLIALVILGYQYFLNQRIAVWLFMLFFGTNALALLLK
                     DIIARHRPMNQLIFDSGYSFPSGHTISTFLLMILVLVVARQRLRRVLSQVVFVIFALV
                     ILASVIFSRLYLENHFLTDILGSLLLGASSYCGLSAIVSLKELQ"
BASE COUNT         1510 a          679 c          776 g         1465 t
ORIGIN      
        1 taaaatatag gggaaaagaa agcgcttgac gaccttttgg acaagtagta agatacaaac
       61 atgggccctg taaattaaaa atactgcagt aaaagtgatt ttagtttaaa ggaggaaatt
      121 tattatgaac gttaaacata tgatgtatct atctggaact ctagtggctg gtgcattgtt
      181 attttcacca gctgtattag aagtacatgc tgatcaagtg acaactccac aagtggtaaa
      241 tcatgtaaat agtaataatc aagcccagca aatggctcaa aagcttgatc aagatagcat
      301 tcagttgaga aatatcaaag ataatgttca gggaacagat tatgaaaaac cggttaatga
      361 ggctattact agcgttgaaa aattaaagac ttcattgcgt gccaaccctg agacagttta
      421 tgatttgaat tctattggta gtcgtgtaga agccttaaca gatgtgattg aagcaatcac
      481 tttttcaact caacatttaa caaataaggt tagtcaagca aatattgata tgggatttgg
      541 gataactaag ctagttattc gcattttaga tccatttgct tcagttgatt caattaaagc
      601 tcaagttaac gatgtaaagg cattagaaca aaaagtttta acttatcctg atttaaaacc
      661 aactgataga gctaccatct atacaaaatc aaaacttgat aaggaaatct ggaatacacg
      721 ctttactaga gataaaaaag tacttaacgt caaagaattt aaagtttaca atactttaaa
      781 taaagcaatc acacatgctg ttggagttca gttgaatcca aatgttacgg tacaacaagt
      841 tgatcaagag attgtaacat tacaagcagc acttcaaaca gcattaaaat aatatttgta
      901 tttttcgtgt gatgctgtcg acttcgtgat tttgtactac catgattgtt atgattaaaa
      961 gatttacgac aatagtcata atagtagaac gatgtcacca ttttaaataa taaagtgatt
     1021 agtcatttga ctaaatttgc caagtatcaa aggaaataaa gattatgact aaaaagataa
     1081 ctgttgtagc attagaaaca ttgattgccc agcataataa tatccatttg atagacgttc
     1141 gtgaagagca tgagtatcgt ggagggcata ttccaggtgc gataaatctt cctttgagtc
     1201 aactcagtca taagtttgaa cagttagata aaaataagga atattatctt gtttgtcaac
     1261 gagggggaag atctattaga gcatgccaat ttctcgagtt gaaaggctac aaagtaatca
     1321 atgtagatgg tggcacagaa gcgtggagag gaaatctaga agtgtaaata tgtcctgtgt
     1381 agtctaccat atttgataat aaaaaaattg acattatctc actattaatg accttttagt
     1441 aaacataaag aaaagtatgt gttttgaaaa ggaggatgat tatccttttc aaaaccatac
     1501 tttttgtgtc ttgtggcttg gtcttaatta atagttgtta ggacaatgtc gtaaatgaca
     1561 aggagaataa aaggccaact agtaagtaat aattttgtgt gttgtttaga gtttattgag
     1621 tcagcctctc gaacgttaga gtgcagactt atacgacggc gagcagtatt cctatgcgta
     1681 aaaaggtaat ataatataaa taagattgct aaaattagca atatattttc aaaataagtc
     1741 ccgtataact cgtttactgt tgatagctgg gtgaaaattt ctacgataaa gttattgcag
     1801 atatgaagga gtagggctac cttaaaggaa taatggtaag cgataaaacc cattatgata
     1861 cccgcaatag atgtagtcaa tagctgagaa atgtcttggt gcattaaacc aaataggatg
     1921 ccagaagtga taatggcaaa ccatttacca tacttggata ataaattcaa tactattcct
     1981 cggtataaaa cttcttcaaa aaaaggacct acaacaacag aataactaat cataagtata
     2041 ctgaatgtgg catcataatt ttgactttcc gattccataa tattgagatg atttagcaca
     2101 gcatcccata ttgtggagac tatattatta gtagtatata taatgcttaa caagaataaa
     2161 atagtggtga aagtgattgt atgatgagtt gccgttagat gtttttttaa atttttaaaa
     2221 cccataaaaa aacaaggaat gagtaaggcg attaaagtac ttgctatacc tatccagaca
     2281 tcattttgta agatttcagc aaatagtgta gtcgccaaac ttaaaacaat agcctgagct
     2341 aaaagtatca gcccgattag gttaagtctc tttttttctt gattcaattg attacctcct
     2401 tattaataac ggtaacgaca aagaaatatt ataatgtata atgtataggt ttctaagtca
     2461 ttagtcaata gggacgagca cttggatact tctaataaga taaaatacat agacaagact
     2521 atggttttgg cttctctaaa gattacctaa aaatttataa gttctcgagc agaaaggatg
     2581 agtttggata ttgagaggat gggcttgacc agctgaaaga aaaatgaacc aagggtaatc
     2641 gcttcgaaaa aagaaggaaa tttcaattag aaacttcctt tttttatcaa tctagtattg
     2701 gcgcgtgcta gggactttat aattcgttat aaaatcttcg atttcagcaa tatcatctga
     2761 gaataggaca ttttctctat cttccagact aagaaaaccc tcactaacca tatgatcaaa
     2821 catatttttt aaatcattaa agtagccatt aacattatat aaaatgcaag gattatcatt
     2881 ttgaccgatg cgagaccaag agataacttc ggaaatttct tcaagggtac caggtccacc
     2941 aggtagagcg ataaaggcat ctccaagtag catcattttt gccttacgtt caggcatatt
     3001 atttactatg ataagctctg ataaattttc atgtgcaatt tcacgatctc ttaaaaatgt
     3061 cggaataaca ccagtagtat agccaccatt tgcaatcaca gtatcagcca tgactcccat
     3121 taagccaact tttcctccac catacaccag agcatgtttg ttttgtgcca tccattgtgc
     3181 caaggcaact gttttttcac tataaacagg attttcccca gtacttgctc cacaaaaaat
     3241 agttattctc atgtttcaaa cctcttttct aatttagtgc gatgtgcgct gataatctct
     3301 tctagtgaga tattatactg atcagcaagt atgaagatat tatctaagac atctcctagt
     3361 tcttctttta tatcatttaa gttttccaaa tcagtttgtt cggtttcgtc aggtctgtca
     3421 cgtccaattt catatttgcg aatggcttga gccaattcgc cgacttcttc tgaaagaaag
     3481 ttacttctaa taaaagaatt gtattgatac cagtttcttt ttttatagaa ttcgctcacc
     3541 catttttgat aatctgtaat atccattgtt ccctccgttt caagtatatt gtagcaatat
     3601 tgctttatta atgaaaattt gaacgctaat taataggaaa agttcgtatt tggtatttct
     3661 tctagtttgt gaatagtcat atttaaactt aagaggttag cgcgtgcttt aagcattttt
     3721 taaatatttt aaaccataat gtatgaagat ttatctcttt attaaagtga ggttatgtag
     3781 tgaacaagaa atcctttttc cagtgtgtta tactacttgt ttctttttta gtgttaacat
     3841 tagcagttaa atctcaaagt gatatgataa gttatttgga taatatcact tcagcttttt
     3901 ttcaaagcat acgaaactcc gatttaacta acttaatgac gataatatca acagttgtca
     3961 gccctttgac aacgtcatta atagcacttg ttatccttgg gtatcaatat tttttaaacc
     4021 aaaggatagc tgtgtggcta tttatgttat tttttggtac caatgctttg gcattgcttt
     4081 taaaagatat catcgcacgt catagaccta tgaatcaact tatttttgac tcaggttata
     4141 gttttcccag tggacatacg atatcaacgt ttttattaat gattcttgtt ttagtggttg
     4201 caagacagag attgagaaga gtattgtcac aagtagtttt cgtaatattt gcattagtaa
     4261 tccttgcatc agttattttt tcaagacttt atttagaaaa tcatttttta acggatattt
     4321 tagggtcgtt gttactagga gcatcgtcat attgtggttt gtctgctatt gtatctttga
     4381 aagagttaca ataaaaatag tagttaccaa tgaattataa aagagccttt
//
LOCUS       DATROD010000127         4296 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.129, whole genome shotgun sequence.
ACCESSION   DATROD010000127 DATROD010000000
VERSION     DATROD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.129"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            50..688
                     /locus_tag="VBC74_001825"
     CDS             50..688
                     /locus_tag="VBC74_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEO6942741.1"
                     /translation="MIMDKSIPKATAKRLSLYYRIFKRFNTDGIEKASSKQIADALGI
                     DSATVRRDFSYFGELGRRGFGYDVKKLMNFFAEILNDHSTTNVMLVGCGNIGRALLHY
                     RFHDRNKMQISMAFDLDSNDLVGKTTEDGIPVYGISTINDHLIDSDIETAILTVPSTE
                     AQEVADILVKAGIKGILSFSPVHLTLPKDIIVQYVDLTSELQTLLYFMNQQR"
     gene            complement(701..1381)
                     /gene="radC"
                     /locus_tag="VBC74_001826"
     CDS             complement(701..1381)
                     /gene="radC"
                     /locus_tag="VBC74_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEO6942742.1"
                     /translation="MYHIELKKEALLPRERLVDLGADRLSNQELLAILLRTGIKEKPV
                     LEISTQILENISSLADFGQLSLQELQSIKGIGQVKSVEIKAMLELAKRIHKAEYDRKE
                     QILSSEQLARKMMLELGDKKQEHLVAIYMDTQNRIIEQRTIFIGTVRRSVAEPREILH
                     YACKNMATSLIIIHNHPSGSPNPSESDLSFTKKIKRSCDHLGIVCLDHIIVGKNKYYS
                     FREEADIL"
     gene            complement(1477..2610)
                     /locus_tag="VBC74_001827"
     CDS             complement(1477..2610)
                     /locus_tag="VBC74_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEO6942743.1"
                     /translation="MKFEKRQVYYVVITFAICYAIQAYWGAVSNILTTLHKAIFPFLM
                     GAGIAYIINIVMSVYERLYIKLFKGSRLLMAIKRSVSMILSYATFIGLIVWLFSIVIP
                     DLISSLSSLLVIDTGALAKLVNNLNENKQISEALNYMGTDKDLVSTLSGYSQQILKQV
                     LSVLTNLLTSVSSIAATLLNVFVSFIFSIYVLANKEQLGRQFNLLIDTYLGSTGKTFH
                     YVRHILHQRFHGFFVSQTLEAMILGSLTVIGMLIFQFPYALTVGVLVAFTALIPVVGA
                     YIGVTIGFILIATESLTEAFLFVLFLILLQQFEGNVIYPKVVGGSIGLPSMWVLMAIT
                     IGGALWGILGMLLAVPVAATIYQIVKDHIIKRQTLRNRARTYR"
     gene            complement(2701..4140)
                     /locus_tag="VBC74_001828"
     CDS             complement(2701..4140)
                     /locus_tag="VBC74_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEO6942744.1"
                     /translation="MMVKQVFPKGFLWGGATAANQCEGAYNVDGRGLANVDVVPTGED
                     RFAIISGQKKMFDFEEGYFYPAKESIDFYHHYKEDLALLAEMGFKTYRMSIAWTRIFP
                     KGDELYPNEAGLRFYENIFKECRKYGIEPLVTITHFDCPIYLIKHYGGWRSRKMIGFY
                     ERLVRALFTRFKGLVKYWLTFNEINMILHAPFMGAGLYFEDGENQEQIKYQAAHHELV
                     ASAIAVKIAHEVDPNSQIGCMLAAGQYYPNTCHPQDYWASMQKNRENYFFIDVQARGK
                     YPNYAKKYFEHLGISIQMTAEDLALLRDYTVDFISFSYYSSRVASGNPTVSEQVQENI
                     FASLKNPYLKSSEWGWQIDPLGLRITLNAIWDRYQKPMFIVENGLGAVDIPDENGYVE
                     DDYRIDYLRQHIAAMRDAIYVDGVNLIGYTTWGCIDLVSAGTGEMEKRYGLIYVDRNN
                     KGEGTLKRYKKKSFYWYKKVIASNGSQIE"
BASE COUNT         1461 a          812 c          647 g         1376 t
ORIGIN      
        1 taactgattc taaatcgaat aataaaattt ttaacaagga gataattttg tgattatgga
       61 taagtctatt cctaaagcaa ctgctaaacg tttatcactg tactaccgta tttttaaacg
      121 ttttaatact gatggcatcg aaaaagctag ttccaaacaa attgcagatg ccctaggtat
      181 cgattctgct actgttcgac gtgatttttc ttattttggt gaactaggac gccgtggttt
      241 tggttatgat gtcaaaaaac ttatgaactt ctttgcagaa atattgaacg atcattctac
      301 aacaaatgtt atgctggtgg ggtgtggaaa tatcggtaga gctctcttgc attatcgttt
      361 ccacgatcgc aataaaatgc aaatttcaat ggcttttgat ttagatagca atgatttagt
      421 tggtaaaaca accgaggatg gaattcctgt ctacggtatt tcgactatca atgaccattt
      481 aatagatagt gatattgaaa ctgctatcct aacagtacct agtacagaag cccaagaagt
      541 tgctgacatc ttagtcaaag caggtataaa aggcatcttg agtttttctc cagttcattt
      601 aacattacca aaagatatca ttgttcagta tgtagattta acaagcgaat tacaaacttt
      661 actttatttc atgaaccagc agcgataaat agttcattat ttataaaata tctgcttctt
      721 ctcgaaaact ataatattta ttttttccaa cgatgatgtg atctaggcag acaattccca
      781 gatgatcaca tgatcgtttt atttttttag tgaaacttaa atcactttca ctgggatttg
      841 gagaacctga gggatgatta tgtataataa tcaaagaagt tgccatgttt ttacaagcat
      901 aatgtagaat ttctcttggc tctgctactg aacgacgtac agtaccaata aaaatagttc
      961 tctgttcgat aatacgattt tgtgtatcca tataaatagc tactaaatgt tcttgttttt
     1021 tatcccctaa ttcgagcatc attttcctcg ctaattgttc actacttaaa atttgctctt
     1081 tacgatcata ttcagctttg tgaatccgtt ttgctagttc tagcatagct tttatttcga
     1141 cggatttaac ctgaccgatt cctttaatgg attgcaactc ctgtaaggat aattgaccaa
     1201 aatctgctaa actgcttatg ttttctaaaa tttgcgttga aatttcaaga acaggttttt
     1261 ctttaatacc tgtacgtaag agaatggcta ataactcctg attactcaat ctatctgcgc
     1321 ctaaatcaac taggcgttct cttggtagta aagcttcctt ttttaattca atatggtaca
     1381 taaaacacct ccagtcataa tattcgtaaa ctaatcaaaa actcatgttt taaatcaata
     1441 aaaaatactt agatgaaaaa tctaagtatt ttttatttaa cgataggttc gtgcacgatt
     1501 tctaagcgtt tgtcgcttga taatatgatc ttttacaatc tgatagatag tagctgcaac
     1561 aggaacagca agtaacatgc ctaagatccc ccataaagca cctccgatag taatagccat
     1621 taaaacccac atagaaggca gtccaatcga tccaccgaca actttcggat aaatgacatt
     1681 tccctcaaat tgttgtaaaa ggatcaagaa aagaacaaac aagaatgctt cagtaagcga
     1741 ttcagtagca attaagatga aaccgattgt aacaccaatg taggctccca caaccggtat
     1801 tagagctgta aaagcaacta aaaccccaac tgttaaagca taaggaaatt ggaagatcaa
     1861 cataccaata accgtcaaac ttcctaaaat catagcttct aaagtttggc ttacaaaaaa
     1921 accatggaaa cgttgatgaa ggatatgacg aacgtaatgg aatgttttgc ctgttgaacc
     1981 taaataggta tcaattaaca aattaaattg acgtcccaac tgctccttgt ttgccaaaac
     2041 gtaaattgaa aaaataaaac taacaaaaac attcagaagt gttgccgcaa tagaggaaac
     2101 tgaggttagt aaatttgtta aaacagataa aacttgcttc aaaatctgct ggctataacc
     2161 acttaaagta gaaactaagt ctttatctgt tcccatataa tttaaagcct cagaaatttg
     2221 tttattttca ttgagattat taaccaattt agcaagtgct ccggtatcaa taaccaataa
     2281 agaactcaaa ctagaaatca aatctggaat gacaattgaa aatagccaga caattaaacc
     2341 aataaaagtt gcataggata aaatcataga aacactacgc ttgattgcca ttaatagtct
     2401 agatccttta aaaagcttta tatataatcg ctcatagact gacattacaa tattaataat
     2461 ataggcaatt ccagctccca tcaaaaaagg aaatattgcc ttatgaagcg tagttaaaat
     2521 attagaaaca gctccccaat aagcctgtat agcatagcaa atagcaaatg ttatgacaac
     2581 ataatagacc tgccgttttt caaatttcat gaaatctcct tattaatctt aatttctttc
     2641 aattattata cactatttta tgaatgagaa aaagagagtt aacagttatt agtctgctct
     2701 ttattctatt tgactgccat tacttgcaat aaccttcttg taccaataaa acgatttttt
     2761 cttgtaacgc ttgagtgttc cctctccttt attattgcga tcaacataaa ttaaaccata
     2821 gcgtttttcc atttcaccag ttcctgcaga tactaaatca atacatcccc atgtagtata
     2881 gccaattaga ttaacaccat ctacatatat agcatctctc attgcagcaa tgtgctgacg
     2941 gaggtaatca atacgataat catcttctac gtaaccattc tcatcaggaa tatctactgc
     3001 tccaagtcca ttttcaacaa taaacatcgg tttttgataa cgatcccaga tagcatttaa
     3061 ggtaatgcgt aatccaagcg gatcaatttg ccatccccat tcagaagatt tcaagtaagg
     3121 attcttaaga gatgcgaaaa tattttcttg aacctgttca ctgacagttg gattacctga
     3181 agctactcgg cttgaatagt aagaaaatga aataaaatca accgtatagt ctcttaacaa
     3241 agcaagatct tctgctgtca tttgaattga aatacctaaa tgctcaaaat attttttagc
     3301 ataattaggg tatttgccac gcgcttgcac atcaataaaa aaataatttt ctctattttt
     3361 ttgcatcgaa gcccaataat cttgtggatg acacgtattt ggatagtatt gacctgcagc
     3421 taacatacat ccgatttgac tatttggatc aacttcatga gcaattttta ctgcaatagc
     3481 cgaagccact aactcatggt gagctgcttg atatttaatt tgctcttgat tttcaccatc
     3541 ttcaaaatat aagccagcgc ccataaaagg agcgtgtaaa atcatattga tttcgttaaa
     3601 ggtcaaccaa tatttaacta accccttaaa acgagtaaat aaagctcgta caagacgctc
     3661 ataaaaacca atcattttac ggctccgcca cccaccgtaa tgtttaataa ggtagatagg
     3721 acagtcaaaa tgtgtgatgg ttaccaaagg ttcaatacca tacttacgac actctttaaa
     3781 aatattttca taaaaccgaa gaccagcttc atttggatat agctcatcac cctttggaaa
     3841 aatacgcgtc catgctattg acatacgata ggttttgaaa cccatttctg caagtaatgc
     3901 aaggtcttct ttataatggt gataaaaatc tattgattct tttgctggat aaaagtatcc
     3961 ttcctcaaaa tcaaacattt tcttttgtcc tgaaataatt gcaaatcgat cttctccagt
     4021 aggaacaaca tctacatttg ctaggccacg tccatcaaca ttgtaagctc cttcacattg
     4081 attggcagca gttgccccgc cccataaaaa accttttgga aatacttgtt ttaccatcat
     4141 ttacccctct tgttttttct tatcataata ttaaaaaaga ccagttactg acattgattc
     4201 gccaaatact ggtactatcc gtgacatgaa ctttctactt agaaggtctt ttcactaatt
     4261 ctgtgtattt aaatacttaa aaatagtatc taaaca
//
LOCUS       DATROD010000128         4231 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.130, whole genome shotgun sequence.
ACCESSION   DATROD010000128 DATROD010000000
VERSION     DATROD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.130"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            155..1894
                     /locus_tag="VBC74_001829"
     CDS             155..1894
                     /locus_tag="VBC74_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEO6942745.1"
                     /translation="MTLFLIMMERAGLIILLAYAFVHIPFIKQTLKQPELKKHQYILL
                     ILFSLFAIISNFTGVEIQSDLSIIPQTLNHIADQSSVANTRVLTIGVSGLIGGPIVGI
                     IVGLLSVFVRYLQGGLAPHIYVISSLLIGLCSGLSGNYLRKNYNKIRVLDAMVVGFGM
                     EILQMICILIFSVDFNQALRLVSFISMPMILSNTLGLGIFISIISSTQKLEEHAKAFQ
                     THQVLELANLTLPYLRKGLTTESCQPVAEIIHKHMDVSAVSLTSQSAILAYVGDGADH
                     HLPNTQILTKLAKRAIDTGKVSVATDKSEIECDHRNCPLSSAIVIPLHIHDVIVGTLK
                     LYFSDAQHMTYVDRQLAEGLGNIFSTQLALGQAEEATRLLQDAEMKSLQAQVNPHFLF
                     NALNTIYGLIRMDSEKARKLVQDFSKVIRANLQRAKQNLIPLHDELEQVNAYLALEEA
                     RFPNMVAFNLDNQTNSDDNLMIPPFTLQVLIENSYKHAFKHINKNNQLKVTIARNNDR
                     LHIIVQDNGIGIPKEKLITLGKKTQISKKGSGTAIENLVRRLNIIYDGQASLKFESND
                     SGTCAIVNIPIKN"
     gene            1906..2640
                     /gene="ltdR"
                     /locus_tag="VBC74_001830"
     CDS             1906..2640
                     /gene="ltdR"
                     /locus_tag="VBC74_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LtdR"
                     /protein_id="HEO6942746.1"
                     /translation="MKVLVVDDEPVARNELIYLLNKYDSNLVIAEAHDMATALAILLR
                     ETFDVALLDIHLRDDSGLQLAEYINKMPKPPLLIFATAYDQYAIQAFEQDARDYLLKP
                     YEFDRLKQAMDRVKGALSTSTIIESVASGPLFKQQYPLTVEDRIYLVSADDILLIEAM
                     QGKLIIQTPDKNYEIDGSLQQWQDKLPSSQFVRVHRSYIVNINAIKTIEPWFNQTLQL
                     HLCNKITVPVSRANVKPLKQMLGIST"
     gene            2796..>4231
                     /locus_tag="VBC74_001831"
     CDS             2796..>4231
                     /locus_tag="VBC74_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEO6942747.1"
                     /translation="MVTFLGGVALLIVGYFTYGRYIEKNFQIDENRQTPAEALRDGYD
                     FVPMPKWKNGMIELLNIAGTGPIFGPILGALYGPVAYIWIVLGCIFAGAVHDYMIGMI
                     SLRNNGAYLPELASRYLGKSMKHVINIFSMLLLILVATVFVVTPANLILSILPAGTLS
                     LPWIIGLIFVYYLISTVLPIDKALGKVYPVFCVILMVSTAAVGFRLLTGGFDMPNLTF
                     ETFKNMHPAGLGIFPALFFTISCGAISGFHATQAPMVSRTTVNEREGRFTFYGMMIAE
                     GVIAMIWAGASMSLFKGQNLYEMIAAGTPSAVVNQVMLMLLGSVIGTIAIIGVIVLPV
                     SSGLSAFRSLRTIVADYIHVKQDTLPKIFAVTIPLYVISFVLTHVDFNLLWRYFNWAN
                     QVTAVIGLLVATRYLILKRRNYWVTFVPAMFMLYAVVVYILSQPIGFNMGLGILTYSL
                     ALVLTGILVGLFWKSGQKQLKTVHPEAFL"
BASE COUNT         1317 a          733 c          752 g         1429 t
ORIGIN      
        1 tcatccttaa gtagttgttt tcataatagt tgttgatgga tatcccctta attgtcttga
       61 gaatacttga tagagagaca ttttaaatga gtaatgaaat gaatcatttt atacctatgt
      121 tataacggac taattaatac tttggagaga tttaatgaca ctctttttaa ttatgatgga
      181 gcgagcgggg ttaatcatat tattagccta tgcctttgtg catattccct ttataaaaca
      241 aactttgaag caacccgaat taaaaaaaca ccagtatatt ctgcttatct tatttagctt
      301 atttgctatt atttctaatt ttacaggggt agaaattcag tcagatttat ctatcattcc
      361 acaaactctt aatcatatag cagatcaatc atcagttgcc aacacacgtg ttttaaccat
      421 tggtgtatca ggtctaatcg gtggccccat agtcggtatt atagttggtc tcttatctgt
      481 attcgttagg tacttgcaag gaggattagc ccctcatatt tatgtcattt cttccttact
      541 aattggttta tgctctgggt tatcaggcaa ttatcttcgt aaaaattata ataaaattcg
      601 tgttttagat gcaatggttg ttggtttcgg gatggaaatt ctacaaatga tctgtatcct
      661 catttttagt gttgacttta atcaggcgct tcgattagta tcttttatca gcatgccaat
      721 gattttaagt aatactttag gtttaggtat atttatatcc attatttcat ctactcaaaa
      781 attagaagaa catgccaaag catttcaaac ccatcaagta cttgaattag caaatttaac
      841 ccttccttac cttagaaagg gactgacaac agaatcttgt caaccagtag ctgaaattat
      901 tcataaacat atggatgtga gtgctgttag cctgactagt caatcagcca ttttagccta
      961 tgtaggagat ggagctgacc accatcttcc taatactcaa atattaacaa agcttgctaa
     1021 aagagcaata gatactggta aagttagtgt cgcaactgat aaaagtgaaa tcgaatgcga
     1081 ccataggaat tgcccactta gttcagcaat tgttattcca ttacacatcc acgatgttat
     1141 agtgggtact ttgaaattat atttttcaga cgcacaacat atgacttacg ttgaccgtca
     1201 attagctgaa ggattaggca atatcttttc cactcagtta gctttgggac aagctgagga
     1261 agcaacaagg ttactacagg atgcggaaat gaagtctctc caagcgcagg ttaatccgca
     1321 ttttcttttt aatgcactca atactattta tggtttaatt cgcatggata gcgaaaaagc
     1381 aagaaagctc gtccaagatt ttagcaaggt tattcgtgct aatcttcaac gggcaaagca
     1441 aaatttaatt cctctccatg atgaattgga acaggttaac gcctatttag cccttgaaga
     1501 agcaagattt cctaacatgg tggcctttaa tttagacaac caaacaaatt cagatgacaa
     1561 cttaatgatt cctcccttta cattgcaggt cttgattgaa aactcttata aacacgcttt
     1621 taagcatatc aataaaaata accagctaaa ggttactatt gcaagaaata atgataggct
     1681 tcatatcatt gtccaggaca atggtattgg gattccaaaa gaaaaattaa taacgcttgg
     1741 aaaaaaaact caaatttcaa aaaaaggttc tggtacagct attgagaatc tcgttcgtcg
     1801 cttgaatatt atttatgatg gacaagcaag tttaaaattt gaatcaaatg attcaggaac
     1861 ttgtgctatt gttaacatcc ccattaaaaa ttagaaaggt attgtatgaa agttttagta
     1921 gttgatgatg aaccagttgc acgtaacgaa ttaatttacc ttcttaataa gtatgattct
     1981 aacctcgtta tagcagaggc gcatgatatg gctactgcat tagctatttt acttagagaa
     2041 acttttgatg tagcactgtt agatatccat ctcagagatg attctgggtt gcaattagca
     2101 gagtatatca ataaaatgcc caaaccacca ttattgatat tcgcgactgc ttatgatcaa
     2161 tatgctattc aggcttttga gcaggatgcg cgtgattatt tgttaaaacc ctatgagttt
     2221 gataggttaa agcaagctat ggatagagta aaaggagcgc taagtacatc tacaattata
     2281 gagagcgtag cttccggtcc tctcttcaag caacagtatc cattgacagt agaagatcga
     2341 atctatctgg tgtcggcgga tgatatcctt ttgattgaag ctatgcaagg aaaactgatt
     2401 atacaaacac ctgataaaaa ttatgaaatt gatggctctc tacaacaatg gcaagataaa
     2461 ctaccatcat ctcaatttgt acgggtacat cgctcttaca ttgtgaatat taatgctatt
     2521 aaaacgattg aaccttggtt taaccaaaca cttcagttac acctttgtaa taaaataaca
     2581 gttcctgtta gcagagcaaa tgtaaaaccc ctaaaacaaa tgttaggcat atctacctaa
     2641 ctaatgaaaa gttattcatc ataaaactta gactattcat ttctaaaaaa caaccattca
     2701 tcttgaaaat aggaaatttc aatttttcaa tgctatacta aagtcatgaa aacgcttaat
     2761 aaaattaagc aaaaaaattt ttaggaggca gtgatatggt aacattttta ggaggggtag
     2821 cccttttaat tgttggttat ttcacgtacg gtagatacat tgaaaagaat tttcaaattg
     2881 atgaaaatcg ccaaacacct gcagaagcat taagggatgg atatgatttt gttcctatgc
     2941 caaaatggaa gaatgggatg atcgagttat tgaacatcgc aggaactgga cctatctttg
     3001 gacctatttt aggagcactc tatggtccag ttgcttatat ctggatagtc ttaggatgta
     3061 tttttgcagg ggctgttcat gattatatga ttggaatgat ttctcttaga aataatggtg
     3121 catatcttcc tgaattagct agtcgctatc ttggaaaatc aatgaagcat gtgatcaaca
     3181 ttttttcaat gctattactt attttagttg caaccgtatt tgtggtaact ccagctaatc
     3241 ttatcctgag tattttacca gctgggacac tatctttacc ttggataatc ggtcttattt
     3301 tcgtttatta ccttatttct acagtattac ctattgataa agcacttggt aaagtttatc
     3361 ctgtattctg cgtcatccta atggttagta cagcagcagt tggcttccgt ttattaactg
     3421 gtggctttga tatgccaaac ttaacttttg aaacctttaa aaatatgcat ccagctggac
     3481 ttgggatatt ccctgcttta ttctttacca tttcatgtgg agccatctct ggtttccatg
     3541 ctacccaagc acctatggtc tcaagaacaa ctgttaatga gagagaaggg cgtttcactt
     3601 tctatggtat gatgattgca gaaggagtaa ttgcgatgat ttgggcaggt gcttcaatga
     3661 gcttgttcaa aggtcaaaat ctctatgaaa tgattgctgc tggaacacct tctgcagttg
     3721 tcaatcaagt aatgttaatg ttactcggta gtgtaattgg aactatcgct atcattggtg
     3781 ttattgttct ccctgtttca tctggtttat cagctttccg tagcttacgt actatcgttg
     3841 ctgattatat ccatgtcaaa caagatactc ttccaaaaat ctttgctgta actatcccat
     3901 tatatgttat ttcatttgtc cttacacacg ttgattttaa tctcctatgg cgttacttta
     3961 actgggcaaa tcaagtaact gctgttattg gtttattagt tgctacaaga tatctcatcc
     4021 taaaaagacg aaattattgg gtaacatttg taccagctat gttcatgttg tatgcagtag
     4081 ttgtttatat tttatctcaa ccaattggat ttaatatggg cttaggcatc ttaacttatt
     4141 cacttgcttt agtgttaaca ggtattcttg ttggactttt ctggaaatct ggtcaaaaac
     4201 aattaaaaac tgttcatcct gaagcattcc t
//
LOCUS       DATROD010000129         4185 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.131, whole genome shotgun sequence.
ACCESSION   DATROD010000129 DATROD010000000
VERSION     DATROD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.131"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..429
                     /locus_tag="VBC74_001832"
     CDS             <1..429
                     /locus_tag="VBC74_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEO6942748.1"
                     /translation="DITIIDSLNKRINFLNILANELELSGVHFFHGRAEDFGQDKVFR
                     AKFDIVTARAVARMQVLAELTIPFLKVNGRLIALKAAAAEEELISAEKALKTLFSQVT
                     VNKNYKLPNGDDRNITIVSKKKETPNKYPRKAGTPNKKPL"
     gene            444..1823
                     /locus_tag="VBC74_001833"
     CDS             444..1823
                     /locus_tag="VBC74_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEO6942749.1"
                     /translation="MKHFFDYKTMSVARKLSISFIAVILLGSILLSLPIFQYANAPKT
                     HYIDHLFTTVSMVCVTGLSVFPISKVYNGWGQIVAILLMQTGGLGLVTLMSLSYYTLR
                     RKMSLNDQTLLQSAITYNSSTDLKKYLYMIFKVTLTLEVLAASILAIDFIPRFGLGHG
                     IFNSIFLAVSAFCNAGFDNLGATSLAQFKLNPLVNIIVCFLIISGGLGFAVWKDLIEA
                     TIQTSHKEPKLIKTFPKRLSNHSKLVLKTTTIILLTGTLLSWLLEFGNFRTIANLSLP
                     KQLMVSFFQTVTMRTAGFSTIDYTQTDFATNLVYIIQMLIGGAPGGTAGGFKVTVIAI
                     LLLLFKAELSGQSQVTFHYRTIPSSIIKQTLSILTFFFIILISGYLLLLELNPHIDPF
                     SLFFEASSALATVGVTMNTTNQLTLGGRIVIMFLMFIGRVGPITVLLSILQKKEKEIH
                     YAETEIILG"
     gene            1836..2507
                     /locus_tag="VBC74_001834"
     CDS             1836..2507
                     /locus_tag="VBC74_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEO6942750.1"
                     /translation="MKTKIIGVLGLGIFGQTLAQELSNFEQDVIAIDSNPENVQAVAE
                     VVTKAAIGDITDLAFLKHIGISDCDTVIIATGNSLESSVLAVMHCKKLGVPQVIAKAR
                     NLVYEEVLYEIGADLVISPERESGQNVAANLMRNKITDVFQIESDISVIEFKIPKSWV
                     GKTVEQLNIRHKFDLNLIGIRKAKNKPVDTEVPINTPLEEGIILVAIANSDAFQRYDY
                     LGYFN"
     gene            complement(2542..3372)
                     /locus_tag="VBC74_001835"
     CDS             complement(2542..3372)
                     /locus_tag="VBC74_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEO6942751.1"
                     /translation="MAKQLIGYQEGYGFLYRLSGASKLLFFILVSVACMTTYDTRFLI
                     VVSLGSLVLFRLAKIKWQQVSFVMTLVVVFAVLNIIMVYLFAPHYGDKIYGSSSLLLK
                     GIGPYDVTSQELFYLFNLILKYFCTVPLALLFLMTTNPSQFASSLNQLGLSYKIAYAV
                     SLTLRYIPDVQEEFYTIRRAQEARGIELSKKSNLVARIKGNLQIVTPLIFSSLERIDT
                     VATAMELRRFGKNKRRTWYSKQSLEKSDIVLIILALASLFVSLYLIHLNHGRFYNPWQ
                     "
     gene            complement(3365..>4185)
                     /locus_tag="VBC74_001836"
     CDS             complement(3365..>4185)
                     /locus_tag="VBC74_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6942752.1"
                     /translation="LDKHTDKLLSVQHLSVSYDLENNTLDDVSFDLYKGQRLAIVGKN
                     GAGKSTLAKALCQFIPNNATLIYNNEDVSQDSIKERAERIGYVLQNPNQMISQAMVFD
                     EVALGLRLRGFSDNDIESRVYDILKVCGLYQFRNWPISALSFGQKKRVTIASILILNP
                     EVIILDEPTAGQDMKHYTEMMSFLDKLSCDGHTIVMITHDMQLMLEYTDRAIVIDNGL
                     IVADDHPINILSNTELLKKTHLKKTSLFALADRLGISPQKLTQWYIDNQGGKNG"
BASE COUNT         1385 a          771 c          685 g         1344 t
ORIGIN      
        1 gatattacta ttattgattc actgaataaa cgcattaatt ttctaaacat attagctaat
       61 gaacttgaac tttcaggagt tcacttcttc catgggcgcg ctgaggactt tggtcaagat
      121 aaagtatttc gagctaaatt tgatatcgta acagctcgtg cagtagctag aatgcaggtt
      181 ctagcagaat tgacaatacc ttttcttaaa gttaatggtc gcttgattgc actgaaagct
      241 gcagctgctg aagaggaatt aatttctgca gaaaaagctt tgaaaacact attttcacaa
      301 gtaactgtaa ataaaaatta taagttacct aatggtgatg accgaaatat cacaatcgtt
      361 agcaagaaaa aagaaacgcc taataaatac cctcgtaaag ctggtactcc aaacaagaag
      421 cctttgtaaa gttgggagct agtatgaaac atttctttga ttacaagaca atgtctgtcg
      481 cacgtaaact cagcattagt tttatcgctg ttatcctatt gggaagtatt ttactttcac
      541 ttccaatttt tcaatatgct aacgccccta aaacacatta cattgatcac cttttcacga
      601 ctgtttcaat ggtatgtgta actggtttat ctgtttttcc tatcagtaag gtttataacg
      661 gttgggggca gattgtagct attttactca tgcaaacagg gggacttggt ttagtaacac
      721 taatgtcgtt gtcttactat accctaagac gtaaaatgtc gttaaatgat cagacccttt
      781 tgcagtcagc gataacttat aacagttcga cagatttaaa aaaatatctc tatatgattt
      841 ttaaagtaac tttaacttta gaagttttag cagccagcat tttagccata gattttattc
      901 ctcgatttgg tttgggtcat ggtatcttta atagtatatt cttagctgtc tcagcctttt
      961 gtaatgccgg ttttgataat ctaggggcaa cgagcttagc tcaatttaaa ctaaatcctt
     1021 tggttaacat tatcgtttgt ttcttgatta tttcaggagg acttggattt gccgtatgga
     1081 aagatttaat tgaagctact atacaaacat ctcacaagga accaaaatta atcaagacat
     1141 ttccaaaacg cctcagcaac cattctaaac tggttttaaa aacaacaacc attattttac
     1201 taacaggaac tttgctttct tggttgttag agtttggaaa ttttagaaca attgctaatt
     1261 tatctctacc taaacaacta atggtcagct ttttccaaac agttacgatg cgtaccgctg
     1321 gattttcaac tattgattat acccaaactg atttcgcaac caaccttgtt tatattatcc
     1381 aaatgttaat tggaggagct cctggtggaa cggcaggagg atttaaagtc actgttatcg
     1441 ccattttact cttactattt aaagccgaac tgagtggcca aagccaagtg acttttcatt
     1501 atcgaacaat tccttccagt attattaaac aaacactatc gattttaact ttctttttta
     1561 tcatacttat tagcggctat ctccttcttc tggaactcaa tccccatata gatccttttt
     1621 ctcttttctt tgaggcatct agtgccttag caaccgtggg tgtaacaatg aacactacca
     1681 atcaattaac tctgggcggt cgaattgtaa ttatgttcct catgtttatt ggtcgagtcg
     1741 gtccaattac cgtactgcta tcaattcttc agaaaaaaga gaaggaaatt cactatgctg
     1801 aaacagaaat tattctaggt tagaaaaggt cttttatgaa aacaaaaatt attggtgtac
     1861 ttggtctagg aatatttgga caaacactcg cacaagaact aagtaacttt gaacaagatg
     1921 ttattgctat tgacagcaat cctgaaaatg tacaagctgt cgccgaagtt gttacaaaag
     1981 cagctatcgg agacattact gatttagctt tcctaaaaca catcgggatc agtgactgtg
     2041 atactgttat tattgctaca ggaaacagtt tagagagctc agtattggcc gtaatgcact
     2101 gtaaaaagtt aggcgtccca caagttattg ctaaagctcg aaaccttgta tacgaagaag
     2161 tactttatga aattggtgct gatttggtta tctctccgga gcgagaatct gggcaaaatg
     2221 ttgctgcaaa cctcatgaga aataaaatta cagatgtctt ccagattgaa tctgatattt
     2281 ctgtcattga atttaaaatt cccaaatcat gggtagggaa aactgtcgaa caattaaata
     2341 tccgtcacaa gtttgatctt aacctcatag gtatcagaaa agcaaaaaac aaacctgtcg
     2401 ataccgaagt tccaatcaat actccacttg aagaaggtat tattctagtg gctatcgcta
     2461 actcagatgc cttccaacgc tatgactatt taggatactt taactaaaga aaataaaaaa
     2521 ctgactatta aagtcagttt tttattgcca tgggttatag aaacgtccat gattaaggtg
     2581 aattaaataa aggcttacaa acagactagc tagagcaagt atgattaaga caatgtcact
     2641 tttttccaaa gattgcttag agtaccaagt acgtctttta tttttaccaa atcgtcgaag
     2701 ttccatagca gttgctactg tatcaatacg ttctagagag ctaaaaatta atggtgtgac
     2761 aatctgcaaa ttccccttaa tgcgtgctac taagttcgat ttttttgata attctattcc
     2821 tcttgcctct tgtgctcgac gtattgtata aaattcttct tgaacatcag gtatatatct
     2881 aagggtcaaa ctaacagcat aagctatttt atatgataaa cctaattgat ttaaacttga
     2941 tgcgaactga ctaggattag ttgtcatcaa aaacaaaaga gctaaaggca ctgtacaaaa
     3001 gtattttaaa atcagattaa agaggtaaaa caactcttgg ctagtcacat catatgggcc
     3061 aattcctttt aacaataaag aagaactacc ataaatctta tcaccataat ggggagcaaa
     3121 aagatagacc attataatat ttaaaacagc gaatacaaca actaaagtca taacaaagga
     3181 cacctgttgc catttgattt ttgctaatct aaataagact agcgatccca atgaaacaac
     3241 aattaaaaat cttgtatcat aagtggtcat gcaagcaaca gaaactaaaa taaaaaatag
     3301 taacttactg gcacctgata gcctgtatag aaagccataa ccctcttgat aaccaattaa
     3361 ttgtttagcc attcttacct ccttgattat caatgtacca ttgtgttagt ttctgtggag
     3421 atatccctaa tctgtctgct aaagcaaata aactcgtttt ctttaaatga gtctttttca
     3481 aaagctctgt atttgaaagg atgttaatag gatgatcatc tgcaacaatc agaccattgt
     3541 cgattacaat tgcacgatct gtatactcta acataagctg catatcatgt gtaatcataa
     3601 caatggtatg tccatcacaa cttagtttat caaggaaaga catcatttca gtataatgct
     3661 tcatatcttg accagctgtt ggttcatcca aaataataac ttctggattt aaaatcagta
     3721 tcgaagcaat cgtaacacgt tttttctgac cgaatgatag agccgatata ggccaattcc
     3781 taaattggta gagcccacaa acctttaata tatcgtagac acgactttct atatcattat
     3841 cactaaaacc tcttaaacgg agacccagag caacttcatc aaaaaccatc gcttgactta
     3901 tcatctgatt gggattttgc aaaacatagc caattcgttc tgctctttct tttatagagt
     3961 cttgactgac atcttcatta ttataaatta gagtagcatt attagggata aattgacata
     4021 acgctttagc taaagtagat ttacctgcac catttttccc aacgatagct aaccgctgac
     4081 ctttatataa atcaaacgaa acatcatcta aagtattatt ctctaaatca tatgacaccg
     4141 acaaatgctg tactgataat aatttatctg tatgtttatc taaat
//
LOCUS       DATROD010000130         4121 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.132, whole genome shotgun sequence.
ACCESSION   DATROD010000130 DATROD010000000
VERSION     DATROD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.132"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            55..1869
                     /gene="glmS"
                     /locus_tag="VBC74_001837"
     CDS             55..1869
                     /gene="glmS"
                     /locus_tag="VBC74_001837"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEO6942753.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVVGDNKSQL
                     VKSVGRIAELQAKVGDSVSGTTGIGHTRWATHGKPTEGNAHPHTSGSGRFVLVHNGVI
                     ENYLQIKETYLTKHNLKGETDTEIAIHLVEHFVEEDNLSVLEAFKKALHIIEGSYAFA
                     LIDSQDADTIYVAKNKSPLLIGLGNGYNMVCSDAMAMIRETSEYMEIHDKELVIVKKD
                     SVEVQDYDGNVIERGSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRKLISTYANESG
                     DMNVDSDIIKSVQEADRLYILAAGTSYHAGFAAKTMIEKLTDTPVELGVSSEWGYNMP
                     LLSKKPMFILLSQSGETADSRQVLVKANEMGIPSLTITNVPGSTLSREATYTMLIHAG
                     PEIAVASTKAYTAQVATLAFLAKAVGEANGKAEAKDFDLVHELSIVAQSIEATLSEKD
                     VISEKVEQLLISTRNAFYIGRGNDYYVTMEAALKLKEISYIQTEGFAAGELKHGTISL
                     IEDNTPVIALISADSTIAAHTRGNIQEVVSRGANALIIVEEGLEREGDDIIVNKVHPF
                     LSAISMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
     gene            2032..2361
                     /locus_tag="VBC74_001838"
     CDS             2032..2361
                     /locus_tag="VBC74_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEO6942754.1"
                     /translation="MSLPNCPKCNSEYVYEDGILLVCPECAYEWNPEEIEEEVGLIVL
                     DSNGTRLSDGDTVTVIKDLKVKGAPKDIKQGTRVKNIRLVDGDHNIDCKIDGFGAMKL
                     KSEFIKK"
     gene            2496..3137
                     /locus_tag="VBC74_001839"
     CDS             2496..3137
                     /locus_tag="VBC74_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEO6942755.1"
                     /translation="MSYILEILPSLLVGAQVTLQVFFIVICLSIPLGIVVAFLMKVHF
                     KPLQWLLTTYIWIMRGTPLLLQLIFFYYFLPSVGISFDRMPAAIIAFTMNYAAYFAEI
                     FRGGIESIPKGQYEAAKVLKFSKFQTVWYIVLPQVFKIVLPSVFNETITLVKDSSLVY
                     ILGVGDLLLESKTAANRDATLAPMFIAGGIYLLLIGLLTILSKQVEKRFNYYK"
     gene            3147..3776
                     /locus_tag="VBC74_001840"
     CDS             3147..3776
                     /locus_tag="VBC74_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEO6942756.1"
                     /translation="MLELKNISKCYGQKEIFKDFNLTVEEGKILSLVGPSGGGKTTLL
                     RMLAGLEKIDSGTIVHDGKEVSVDHLETLNLLGFVFQDFQLFPHLTVLDNLILSPVKT
                     MGLSKELAKEKALVLLERLGLKDHALVYPFSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQEVEKLILQNRETGMTQIVVTHDLQFAESISDTILKINPK"
     gene            3790..>4121
                     /locus_tag="VBC74_001841"
     CDS             3790..>4121
                     /locus_tag="VBC74_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6942757.1"
                     /translation="MLTHKNILLTIIFGLFMIILSACGMSNKEMAGIDNWEHYQKEKK
                     ITIGFDNTFVPMGFESRSGDYTGFDIDLANAVFKEYGISVKWQPINWDMKETELNNGN
                     IDLIWNGYS"
BASE COUNT         1348 a          609 c          829 g         1335 t
ORIGIN      
        1 aaaagaaagt ttagatagaa agtataagag aaagctctta ttggtaataa taatatgtgt
       61 ggtatcgtag gtgtagttgg aaatacaaat gcaacggata ttttaattca aggcttagaa
      121 aagctagagt accgaggata tgactcagca ggtatttttg tagtaggtga taacaaaagt
      181 caacttgtga agtcagtcgg tcgaatcgcg gagcttcagg caaaagtagg agattctgtt
      241 agtggtacga caggtatcgg tcatacacgt tgggcaaccc atggtaaacc tactgaagga
      301 aatgcacatc ctcacacttc aggatctggt cgttttgtat tggtacacaa tggtgttatt
      361 gaaaattacc ttcaaattaa agaaacttac ttaacaaaac ataacctcaa gggtgaaact
      421 gatacagaaa tagctattca tttagttgaa cactttgttg aagaagataa cttatcagtg
      481 cttgaagcct ttaaaaaagc cttacatatt atagaaggat catacgcatt tgctcttatt
      541 gattctcaag atgctgatac gatttacgta gctaaaaata aatcacctct tctaataggt
      601 ttgggaaatg gttataatat ggtgtgttca gatgccatgg ctatgatacg tgagacaagt
      661 gaatatatgg aaatccacga taaagaactc gttattgtta aaaaagatag cgttgaagtt
      721 caagattatg atggtaatgt cattgaacgt ggtagctaca cagcagagct tgacttatca
      781 gatattggta aggggactta tccattctac atgcttaaag aaattgatga gcaaccaact
      841 gtgatgcgta agttgatttc aacttatgcg aacgaatctg gagatatgaa tgtggattct
      901 gatattataa aatcagtcca agaagcagat cgtctttaca tattggctgc aggaacgtca
      961 tatcatgctg gttttgctgc taagaccatg atcgaaaaat taactgatac acctgttgaa
     1021 cttggagttt cttcagaatg gggttacaac atgccacttt tgagtaagaa gccaatgttt
     1081 attttactta gtcaatcggg agaaacagca gatagtagac aagttttagt taaagcaaac
     1141 gaaatgggaa tccctagttt aactataaca aatgtgcctg gctcaacact ttctcgtgaa
     1201 gcaacctata cgatgctcat tcatgcagga cctgaaatcg ctgttgcttc tacaaaagca
     1261 tatactgctc aagttgcaac gttagctttc ttagcaaaag cggtaggaga agcaaatggt
     1321 aaggcagaag caaaagattt tgatttggtt catgaattgt caattgtagc acaatcaatt
     1381 gaagcgacct tatcagaaaa agatgttatc tctgagaagg tagagcagtt gttaatctcg
     1441 acacgtaatg ccttctacat tggtcgtggt aacgactatt atgttacaat ggaggctgca
     1501 ctgaagctaa aagagatttc ttatattcag acagaaggtt ttgctgcagg tgaattaaaa
     1561 catggtacaa tttcgttgat tgaagacaat actcctgtta ttgctttaat atctgctgat
     1621 tcaactattg cagcgcatac tcgtggaaat atccaagaag ttgtttctcg cggagcaaat
     1681 gctttgatta ttgtagaaga agggttggaa cgtgaaggtg atgatattat tgttaataaa
     1741 gttcacccat tcttatcagc catttcaatg gttattccga ctcaattaat tgcttactat
     1801 gcttcattac aacgtggctt agatgttgat aaaccacgta atttggcaaa agctgtaact
     1861 gttgaataga ctcgttacac taaaatacgc taatattaac tacaaaatat taaaaattgt
     1921 aaaaaagcct atttttgaag gaaaataggc tttttcctat tgtcaagaag cctataatca
     1981 gttagaatgt tacttgctga ctattttatt aattaattta aggagagaaa aatgtcatta
     2041 ccaaattgcc ctaagtgtaa ctctgaatat gtctatgaag acggtatttt actggtttgt
     2101 ccagaatgtg cttacgagtg gaatccagaa gaaatcgaag aagaagtagg tttaattgta
     2161 ttggatagca atggaacgcg cttatcagat ggagatactg tgacagttat taaggattta
     2221 aaagtaaaag gagcgcctaa ggatattaag caaggcactc gtgttaaaaa tattcgttta
     2281 gttgatggcg atcataatat cgattgtaaa attgatggtt ttggagctat gaaactaaaa
     2341 tctgagttta taaaaaaata gctttcaggc aattggcgac ggtcaattgc tttttgaata
     2401 tgacttgatt tcatgcattt ttattaaaaa agctctatga cattggtttg aattttgata
     2461 taatgagaac atctattttt tatttagggg ggaccatgtc ttatatttta gaaatcttac
     2521 ctagcttatt agtaggtgcg caagtgacat tgcaagtttt ctttattgtt atttgtttat
     2581 caattccact tggtattgtt gtagccttcc taatgaaagt tcattttaag ccgcttcaat
     2641 ggctactgac aacttatatt tggattatgc gaggcacgcc tctgttatta cagcttatct
     2701 ttttttatta ctttttacca agtgtaggaa tttcttttga tcgtatgcca gctgcgatta
     2761 ttgcttttac gatgaattat gcagcttatt ttgcagagat ttttcgagga ggtattgagt
     2821 ctattcctaa agggcaatat gaagctgcaa aggtattaaa gttttctaag tttcaaacgg
     2881 tttggtacat agtattacct caagtcttca aaatagtctt accgagtgtt ttcaatgaaa
     2941 cgattactct agtgaaagat tcctcattgg tttacatact tggtgtgggc gacttacttc
     3001 tagaaagtaa gacagccgca aatagggatg caacacttgc accaatgttt atagcaggtg
     3061 gtatttacct attattaatt ggtcttttaa ctattttatc taaacaagtt gaaaagcgtt
     3121 ttaactacta taaataagga gggacgatgt tagaattaaa aaatatttct aaatgctatg
     3181 ggcaaaaaga aatctttaaa gatttcaatc tgactgtaga agaaggtaaa attctctctt
     3241 tagtaggacc ttctggaggt ggaaaaacaa cactattacg tatgttagct ggattggaaa
     3301 agattgattc agggactatt gttcacgatg gtaaagaagt atctgttgat catttggaaa
     3361 cactaaatct tttaggattc gtcttccaag actttcaatt attcccacat ttaacagtat
     3421 tagataattt gatcctctct cctgttaaaa cgatgggatt atcaaaagaa cttgctaaag
     3481 aaaaagcctt agtattactt gaacgcttgg gacttaaaga ccatgcttta gtctatccat
     3541 tttcattatc tggggggcaa aagcaacgtg tcgcactagc tcgtgcgatg atgattgatc
     3601 cacagattat tggttatgat gagccaacta gcgctcttga tccagagttg cgtcaagaag
     3661 tagaaaaact aattttacaa aatagagaaa caggtatgac acaaattgta gtaacacatg
     3721 atcttcaatt tgctgaaagt atatctgata cgattctcaa aattaatcct aagtagactt
     3781 gggggtagat tgttgactca caaaaatata ttattaacca ttatatttgg attatttatg
     3841 attatattat cagcatgtgg tatgtctaat aaggaaatgg ctggtattga taattgggaa
     3901 cattatcaaa aggaaaagaa aattactatt ggatttgata atacttttgt tcctatggga
     3961 tttgaaagtc gttctggtga ctataccggc tttgatattg atttagctaa tgctgttttt
     4021 aaagaatacg gtatttcagt gaaatggcag cctattaact gggatatgaa agaaactgaa
     4081 cttaataatg gtaatataga ccttatttgg aatggttatt c
//
LOCUS       DATROD010000131         3891 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.133, whole genome shotgun sequence.
ACCESSION   DATROD010000131 DATROD010000000
VERSION     DATROD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.133"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            1..639
                     /locus_tag="VBC74_001842"
                     /pseudo
     CDS             1..639
                     /locus_tag="VBC74_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645527.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            636..1970
                     /locus_tag="VBC74_001843"
     CDS             636..1970
                     /locus_tag="VBC74_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEO6942758.1"
                     /translation="MNSTLSILEIALTIILTIFLFSKISGRQLTLIEIGICIALRLGL
                     VILFIAANTIINLSFLKYWLLPIYMLSLAFLLLRPISKTLVVFYGLFPIVLRNLCSRS
                     LAFFVFPLFNIDFHTTNQGFWGYFVDVLSTLCVLLFLRWMQYDFKTLRSSEVSPIDKR
                     ILQFTNGCMVSYYLLMQFSTYLEYELAINTVIYRQFLLVVYWIIFTGAIASLDRRLRN
                     SLQERLAVQRELQLANMIDYSHHIEGLYKEVRSFRHDYSNMLTTLKLGIDNEDISIVK
                     SVYQSIFKESNTHFRHPKYNIDRLVNVDNPALKSLLAAKLSQAIDNHIMVSIEVPDSI
                     KPQGMELIDFITIVSVLWDNAIEASLETDDPKIAMSYFTLKHKQIFIIENSTKEKAID
                     TSTIFNYEISSKGRNRGIGLYNVRGVINRYPNISIATSSQDFSFCQTFEIIL"
     gene            complement(2059..2223)
                     /locus_tag="VBC74_001844"
                     /pseudo
     CDS             complement(2059..2223)
                     /locus_tag="VBC74_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632309.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2512..2685
                     /locus_tag="VBC74_001845"
     CDS             2512..2685
                     /locus_tag="VBC74_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEO6942759.1"
                     /translation="MKHYHMLKVALVIDGYTVRTGKHTYTSAWHLSENTAAANARNRM
                     GLYPGSVCRVYLV"
     gene            2754..3602
                     /locus_tag="VBC74_001846"
     CDS             2754..3602
                     /locus_tag="VBC74_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6942760.1"
                     /translation="MLLQLDNITKSYGKKIVLNQISYQFTPGLYGLLGANGTGKTTLL
                     NLMSHFTFADSGNIYWNGQEQSEEFYRHIGFLPQHFRYYDQFTGIAFLNYIATLKGVD
                     KKKAKPEIPRLLELVGLGDVGKKKISSYSGGMKQRLGIAQALINDPEILILDEPTVGL
                     DPKERVKFRHILSQLSTNKIIILSTHIVSDVEAVAKEIIVLKNGKFIEQGNTAQLLKT
                     IEGKVWEITTEPDLSQIPNIAIVNEKVISDSLVFRVVSDICPSDSAQLVVPTLEDFYI
                     YHFRGE"
     gene            3608..>3891
                     /locus_tag="VBC74_001847"
     CDS             3608..>3891
                     /locus_tag="VBC74_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942761.1"
                     /translation="MISIFPFELKKLLQRKAALGAFIIFLLAIFGLFYQHFFVGQISG
                     YSADKIHGREAVAINRRIAEKYSGDLSEERVTNIIKNYVNPPAGRPPIFDV"
BASE COUNT         1256 a          599 c          702 g         1334 t
ORIGIN      
        1 aaaccagttc aattattgga atcaatctgt gaaaaaggta tgcataattt atatttctta
       61 gacattgaaa taaaaaatga tgagcagagg ggcttagagg tcgcaaaaca aattagacaa
      121 attgatcctt atgctcagat tgtttttgtt acaactcatt cggaattaat gcctttaacc
      181 tttcgttatc aggtatcagc tttagactat atcgataaag gactttcaca ggaagcattt
      241 agcagaagga ttgaagacgt tttactatat gctaataatg cttgcagtaa aaacttacta
      301 gaagattctt tttattttaa atcacgctat agtcaagtac aagtaccttt taacgatatc
      361 ctttatatag aaacgtcatt aaggtcacat agagttgtcc tttatactgc aaaagacaga
      421 atggaattca cagccacttt gggggatatt ttaaaacaag aacctaggct ctttcaatgt
      481 catcgctctt ttttagtaaa tcccttgaat atttttaaag tggataggtc aaataaacta
      541 atctattaaa aaaatggttc gaattgtttg atttctcgta ataaaataaa agatattatg
      601 gtagtaatag aggaaaatca aagaggtaga ggaatatgaa ttctactcta tccatcttag
      661 agatagcttt aacgataata ttaacaatat ttctcttttc taaaatcagt ggacgtcaat
      721 tgactttaat agagattgga atttgtattg ctctgagatt aggacttgta attttattta
      781 tagcagctaa tactattatt aatctttcat ttctaaagta ctggcttctc cccatctata
      841 tgttaagtct ggctttcttg ttgctaagac ctatatctaa aactttagta gtcttttatg
      901 gcttgtttcc aattgttctc agaaatcttt gttcacgttc tttagctttt tttgtctttc
      961 ctttgtttaa tattgatttt catacaacca accaggggtt ttggggatat tttgttgatg
     1021 tcttgtctac tctttgtgtt ctcctatttt taagatggat gcaatacgac tttaaaactt
     1081 tgcgaagtag tgaagtttcg cccatagata agagaattct ccaatttacg aatggttgta
     1141 tggtcagtta ttacttactg atgcagtttt caacttattt ggaatatgaa ttagccatca
     1201 atactgttat ttaccggcaa tttttgcttg ttgtttattg gattatattt acgggagcaa
     1261 tagccagttt agaccggaga cttcgaaatt ctttacagga gaggttagct gttcaaagag
     1321 aactccaatt ggctaatatg atagattata gccaccatat tgaggggttg tataaagaag
     1381 ttaggagctt ccgacatgat tatagtaaca tgttaacgac cttgaaacta gggattgata
     1441 atgaagatat atctattgtc aaatcagttt atcagtctat ctttaaagaa tctaatacac
     1501 atttccgaca tcctaagtat aatatagacc ggttggttaa tgtagataac ccagccttaa
     1561 aaagtctttt agcagccaag ctttcacaag cgatagacaa tcatattatg gtatctatag
     1621 aagtaccaga cagtattaaa cctcaaggga tggaactgat tgattttatt accattgtat
     1681 ctgttttatg ggataacgct attgaggcat ctttagagac agacgatcct aagatagcca
     1741 tgtcttattt tacattaaaa cataaacaaa tttttatcat agaaaattca actaaggaaa
     1801 aggcaataga tacgtcaacg atattcaatt atgagataag tagtaagggg cggaatagag
     1861 gaataggtct ttacaatgtc aggggagtta tcaatcgtta tccaaatatt tctattgcaa
     1921 caagtagcca agattttagt ttctgccaaa ctttcgaaat catattatag ttttaaaaag
     1981 ttcaatttat ctaagtttat tattttctta ataccatcaa tccaaccacc accagtgata
     2041 ttatttagtt gtttcggcga taattcctga agctgtagtt ccatctcgat tagttttggc
     2101 tagttctcgt aagtggttta ataaatattt tgaaccatag aactttgcga ttgaagcaag
     2161 tgcagttact ccacaatcct tagtatcaac ctgacgcacc aatgcttttt tgtacgtcaa
     2221 cattgtgtaa ttcctttctt cgttatcgta ttaatatttt gccataaaaa tatatactta
     2281 ttaaaaatag cttaaacagc tattgcaacg cgtccaatgg tttctataag gatataaagg
     2341 tagatagtac ttttctgttt tgaggcgatt caacgacatt tcagggaata aacgtttcta
     2401 tttttatttg aatgctatat taaatgtgtc aagttgcaac agacaaaaat tttatatagg
     2461 agatattcaa aataaatgta aaatcaattg aacaattaga agttatggat aatgaagcat
     2521 tatcacatgt tgaaggtggc cttggtcata gatgggtata cagtaagaac cggtaaacat
     2581 acttatacaa gtgcatggca tctttctgaa aatactgctg cagcaaatgc aagaaatcgt
     2641 atgggccttt accctggaag cgtttgtcgt gtctatcttg tataattgat tttttattta
     2701 agcgataggg aatcttcctt gccgcttaag tttgtatagg gagagacaat actatgttat
     2761 tacagctaga taatatcact aaaagctacg gaaaaaaaat agtacttaac cagatttcct
     2821 accagtttac tcctggtttg tatggactct taggagcaaa tggtactggc aaaacaacct
     2881 tattaaattt gatgagccac tttacattcg cagatagtgg gaatatttat tggaatggac
     2941 aggagcagtc ggaagaattt tatcgtcaca ttggtttttt accgcaacat tttcgctatt
     3001 atgatcaatt tactggcatt gctttcttaa attatatcgc taccttaaaa ggtgttgata
     3061 aaaagaaagc taaaccagaa attcctagat tactagagct tgttggttta ggtgatgtag
     3121 gtaaaaagaa gatttcatca tattccggtg gtatgaagca acgtttaggc attgctcaag
     3181 cacttatcaa tgaccctgaa atacttattc tggatgaacc aactgttgga cttgatccta
     3241 aggaacgggt taaatttcgc catattttga gtcagttatc caccaataaa attatcattt
     3301 tatcaacgca tattgtgtcg gatgtggaag ctgttgccaa agaaattata gtattaaaaa
     3361 atggtaaatt tatcgaacag ggcaacaccg cacagttgtt gaaaactatt gagggaaaag
     3421 tgtgggaaat aacgactgaa ccagatttat ctcagatacc caatattgct attgttaatg
     3481 aaaaagtcat ttcagatagt ctggtatttc gtgtagtgag tgatatttgt ccgagtgatt
     3541 cggcacagtt ggttgttccg actctagaag atttctacat ctatcatttt agaggggagt
     3601 aagtaagatg atatcaattt ttccttttga attaaaaaaa cttctacaac gtaaggcagc
     3661 attgggggct tttattatct tccttcttgc tatttttggt ctattttacc agcatttttt
     3721 tgttgggcaa attagtggct attctgcaga taaaatacat ggtcgagaag cggttgcaat
     3781 taatcgtaga atagctgaaa aatattcagg ggatttatct gaagaacgag taacaaatat
     3841 tattaaaaac tatgttaatc cgccagcagg acgcccccct atatttgatg t
//
LOCUS       DATROD010000132         3823 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.134, whole genome shotgun sequence.
ACCESSION   DATROD010000132 DATROD010000000
VERSION     DATROD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.134"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(86..601)
                     /gene="lacB"
                     /locus_tag="VBC74_001848"
     CDS             complement(86..601)
                     /gene="lacB"
                     /locus_tag="VBC74_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEO6942762.1"
                     /translation="MKIAVGCDHIVTYDKIAVVDYLKTKGYEVIDCGTYDNIRTHYPI
                     YGKKVGEAVASGKADLGVCICGTGVGINNAVNKVPGIRSALVRDLTSAIYAKEELNAN
                     VIGFGGKITGGLLMTDIIEAFIRAKYKPTKENKVLIEKIAEVETHNAHQEENDFFTEF
                     LDKWNRGEYHD"
     gene            complement(622..1046)
                     /gene="lacA"
                     /locus_tag="VBC74_001849"
                     /pseudo
     CDS             complement(622..1046)
                     /gene="lacA"
                     /locus_tag="VBC74_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
     gene            complement(1296..3746)
                     /locus_tag="VBC74_001850"
     CDS             complement(1296..3746)
                     /locus_tag="VBC74_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEO6942763.1"
                     /translation="MTLTFGKPSITIRRLGVITSTYMMLGFILLCGLLHPSTIYSEEI
                     KSDINSFISIDDEKTTAPIFDSTLDKLTNSTEERQLELDSDTSDGLIVDDQKDKDMVV
                     VLGDSDSQEKLSPPLKSEIATEENRKQANLAELNATQPNNRTTYIIPESSHSIAEQQR
                     FLIESKGSSVALLNSDEFRKTAGEDRGFERDKLRSLDIIPKGDLSTSNVIGNTDIASQ
                     ISLGFKKNAMQEHHLTKTFSQKDGKLSSVIEGMLAIGKEKVEKEIKYSGNLWQKLKAK
                     AHCLVCCVDNLNFEDIKSYFQYYCHLNHQLKLPKGAILSAKTEVYRGGDFGRKNKDNV
                     FGYRIPSLLKTQKGTLLAGADERIEQACDWGNIGMVIRRSEDDGVTWGKRETIVNLRN
                     NPRVPLVTSGDYSGSPINMDMALVQDTSSKTKRIFSIYDMFPEGRGVISIANTPEKEY
                     TQIGGQSYLNLYNNGKKSKVFTIRDKGIVYNFKGKKTDYHVITETTKSDHSNLGDIYK
                     GKQLLGNIYFTKHKTSPFRLAKSSYVWMSYSDDDGRTWSSPRDITASLRQKGMKFLGI
                     GPGKGIVLKWGPHAGRIIIPAYSTNWKSHLRGSQSSRLIYSDDHGKTWHTGKAVNDNR
                     ILSNGEKIHSLTMDNKKEQNTESVPVQLKNGDIKLFMRNLTGNLEVATSKDGGETWQN
                     HVKRYKEIHDAYVQLSAIRFEHDKKEYILLVNANGPGKKCQDGYARLAQVNRNGSFKW
                     LYHHHIQDGSFAYNSVQQLNNDQFGVLYEHREKHQNSFTLNYKVFNWSFLSQNTEKQG
                     TLWEKMAANWHVLFKFYL"
BASE COUNT         1128 a          787 c          550 g         1358 t
ORIGIN      
        1 tacagtatcc atgttaaagt tttctaagca gtacgaaata tcaatagaag ggttcaaagt
       61 tactgttaaa atcatagata tctccttagt cgtgatattc accacgattc catttatcta
      121 agaactctgt gaaaaagtca ttctcttctt gatgggcatt atgagtctca acctcggcta
      181 tcttttcaat taatactttg ttttcttttg ttggtttata tttagctctt ataaacgcct
      241 cgatgatatc tgtcattaac agcccaccag taattttccc accaaatccg ataacgttag
      301 catttaattc ttctttagca tagatagctg atgtcaaatc acgtaccaga gctgagcgaa
      361 ttcctggaac tttattaaca gcattattaa taccaactcc agttccacaa atacatacac
      421 ctaagtcggc ttttccacta gcgacagctt ctcctacttt cttaccataa ataggatagt
      481 gagtacggat gttatcataa gttccacaat caataacctc ataccctttt gtttttaaat
      541 aatccacaac tgcaattttg tcataagtta caatatgatc acaaccgacc gcaattttca
      601 tctgctttac ctctcttacg cttagcacat tttattcaac atatcgaccc gaatttgatg
      661 acgacctgca tcgtaagtcc catcaacaaa accctctact atatgtttag caaccccgga
      721 ccaacaatct ctgaacctaa agtaatcatc ctagcgttat tatgagctct agtcatataa
      781 gctgagcgtt catctgaaac ttctgctgca atcatcccct taaccttagt ggctaccata
      841 aaaggaccag caccgtaagc atcaaccatt attcctaagt tatcttcttt ttgattcact
      901 ttagcaacga ttgctaaggt attatcaacg aaatctttgc tagtatccgt taaatcaata
      961 atttccttac ctaaacgtgt aaggtgttgt ctaatcactt ccttaagttc aacaccatgc
     1021 gcatccgccc caataataat tgtcatttct ttctccttat ttcactttta atacaattgt
     1081 atgtagttgg aagatattgt ttgttttttg tttgtaatat catcatatat cttatttttg
     1141 tttttgtcaa tgttttgttt ttgtttttgt ttgaaataaa caaaaaaaac tccgtgataa
     1201 ctatggcaac tagaattcac tactagctag ctatcacgaa gtttgatact tctcattatt
     1261 tttatttgta ggaacggtta ttccgcaagc cataatcata aataaaattt aaacaaaaca
     1321 tgccaatttg ctgccatttt ctcccataaa gtgccttgct tctctgtatt ttgactaaga
     1381 aaactccaat taaaaacttt gtaatttaaa gtaaaactat tttgatgttt ttctctatgt
     1441 tcataaagga caccaaattg atcattatta agttgttgaa cagagttgta agcaaacgaa
     1501 ccatcttgaa tgtgatggtg atataaccac ttaaaactac catttcgatt aacttgcgct
     1561 agacgtgcat atccatcttg gcacttcttc cctggcccat tagcattcac taataaaata
     1621 tactcttttt tgtcatgctc aaagcgaata gctgatagtt ggacgtaagc atcatgaatt
     1681 tccttatatc gtttaacatg gttttgccaa gtctccccgc cgtctttact tgtggctact
     1741 tctaggttac cagttagatt cctcataaat aacttaatgt ccccattttt caattgaacg
     1801 ggtacggatt ctgtattttg ttctttttta ttatccattg ttaaggagtg aattttttca
     1861 ccattagaaa gtatacggtt atcattgact gcttttccag tatgccacgt ttttccatgg
     1921 tcgtctgaat aaattaggcg tgaagattgt gaacctctta gatgagattt ccaattcgta
     1981 gaataggcag gaataataat acgaccagcg tgtggccccc attttaaaac tatacctttt
     2041 ccaggtccta ttcccaaaaa tttcatgcct ttctgacgaa gacttgctgt tatatctcta
     2101 ggtgatgacc atgtcctacc atcatcatcg ctatatgaca tccacacata gcttgatttt
     2161 gctaaacgaa atggtgacgt tttatgtttt gtaaaatata tatttccaag tagctgtttt
     2221 cccttataaa tatcccctag atttgaatgg tcacttttag tagtttctgt tataacatga
     2281 taatcagtct ttttcccttt aaaattatat acaatacctt tgtcacggat agtaaaaacc
     2341 ttcgatttct ttccattatt atataaatta agataagact gtcctccgat ttgggtatat
     2401 tctttttcag gtgtgttagc aatactaata acgcctcttc cttctggaaa catatcatat
     2461 attgaaaaaa tacgtttcgt cttggagcta gtatcttgaa ctaatgccat atccatatta
     2521 ataggtgagc cactatagtc accactagta actagcggaa ctctagggtt attacggaga
     2581 ttgacaatag tttctctttt tccccaagtg acaccatcat cctcactacg gcgaataacc
     2641 attcctatgt ttccccaatc acaagcttgc tcaattcttt catcagctcc cgcaagtaaa
     2701 gttccttttt gggttttcaa taatgagggg atacggtaac caaacacatt atctttattt
     2761 tttctcccaa aatctcctcc cctatatact tctgttttag cagaaagtat agcaccttta
     2821 ggtaatttga gctgatggtt tagatgacaa taatattgaa aataagattt tatatcttca
     2881 aaattcaaat tatcaacaca gcaaacaagg cagtgtgcct tagcttttaa tttttgccat
     2941 aaattaccac tatattttat ttctttctct actttctctt tgccaatagc aagcatcccc
     3001 tctataacag acgataactt tccatccttt tgagagaatg ttttagtaag atggtgttcc
     3061 tgcatcgcat tctttttaaa gcccaacgat atctgactag caatgtccgt attacctatg
     3121 acattacttg tcgataaatc tcccttagga atgatatcca aagacctcaa cttatccctt
     3181 tcaaaacctc tatcctctcc cgctgtcttt ctaaattcat cgctattaag taatgcaacc
     3241 gaagaaccct ttgattctat caggaatctc tgttgttctg caatggaatg actgctttcg
     3301 ggtataatat aggtagttct attattaggt tgagttgcat ttagttctgc taaatttgcc
     3361 tgtttacgat tttcttctgt cgctatttcc gattttagag gaggtgacaa tttttcttga
     3421 ctgtcagaat cacctaagac tactaccata tctttatctt tttgatcatc tactattaaa
     3481 ccgtccgatg tatcagaatc taattctaac tgcctctcct ctgttgaatt agtaagctta
     3541 tctaaagtag agtcaaatat gggagcagta gttttttcat catctattga tataaaagag
     3601 tttatatcag attttatttc ctcactataa attgtactag gatgcaaaag tccacataat
     3661 aagataaaac ctagcatcat atatgtacta gttatcactc ctagacggcg tatcgttata
     3721 gaaggtttcc caaaggttaa tgtcatagaa actccaagtc ttaaaatatt ggttgcatac
     3781 atcaactcta gaaaaatggg agttcagtaa tgtttccaaa aag
//
LOCUS       DATROD010000133         3805 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.135, whole genome shotgun sequence.
ACCESSION   DATROD010000133 DATROD010000000
VERSION     DATROD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.135"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..570
                     /locus_tag="VBC74_001851"
     CDS             <1..570
                     /locus_tag="VBC74_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6942764.1"
                     /translation="KNKILTMVALTVLTCATYSSIGYADTSDKNTDTSVVTTTLSEEK
                     RSDELDQSSTGSSSENESSSSSEPETNPSTNPPTTEPSQPSPSEENKPDGSTKTEIGN
                     NKDISSGTKVLISEDSIKNFSKASSDQEEVDRDESSSSKANDEKKGHSKPKKELPKTG
                     DSHSDTVIASTGGIILLSLSLYNKKMKLY"
     gene            complement(604..1845)
                     /locus_tag="VBC74_001852"
     CDS             complement(604..1845)
                     /locus_tag="VBC74_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEO6942765.1"
                     /translation="MVLQDFDNLLKKYAQLIISKGLNVQKGHTLALTIDVEQVHLARL
                     LTEVAYEKGASEVIVDYTDDFITRQRLLHASDEVLTNVPQYTVDKSLALLNKKASRLV
                     VKSSNPNAFATVDPKRLSKTTRATAIALEEQSRAIQANKVSWNVAAAAGREWAALVFP
                     ELKTSDQQVDALWDTIFKLNRIYEDNPIAAWDAHEAKLLEKATRLNQEQFDALHYTAP
                     GTDLTLGMPKNHIWEAAGSLNAQGETFIANMPTEEIFSAPDYRRADGYVTSTKPLSYA
                     GVIIENMTFTFKDGKIINVTAEKGQETIQRLIEENDGARSLGEVALVPHKTPISLSGL
                     IFFNTLFDENASNHLAIGSAYAFNVEGGTEMTSQELDEAGLNRSSTHVDFMIGSEQMD
                     IDGIRADGTAVPIFRNGEWAI"
     gene            2023..2151
                     /locus_tag="VBC74_001853"
     CDS             2023..2151
                     /locus_tag="VBC74_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEO6942766.1"
                     /translation="MAFGDNGQRKKTGFEKLTLFVVILMVLVTVGGLVFGAISAIM"
     gene            2212..3525
                     /gene="obgE"
                     /locus_tag="VBC74_001854"
     CDS             2212..3525
                     /gene="obgE"
                     /locus_tag="VBC74_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEO6942767.1"
                     /translation="MSMFLDTAKISVKAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFKVNEGLRTLMDFRYNRNFKAKAGEKGMTKGMHGRGAEDLIVSLPPGTTVRDANT
                     GKVITDLVEHDQEFVVARGGRGGRGNIRFATPRNPAPEIAENGEPGEERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVVSAAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYDDYISINNELETYNLRLME
                     RPQIIVANKMDMPDSEENLAAFKEKLAANYDEFDDMPMIFPISSLAHQGLENLMDATA
                     KLLANTEEFLLYDETDMQEDEAYYGFNEDERPFEITRDDDATWVLYGDKLEKLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDIVRIGNFEFEFVD"
     gene            3551..3709
                     /locus_tag="VBC74_001855"
     CDS             3551..3709
                     /locus_tag="VBC74_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942768.1"
                     /translation="MGDKPISFRDKDGNFVSAADVWNAEKLEELFNTLNPNRKLRLER
                     EKLAKEKG"
BASE COUNT         1213 a          631 c          798 g         1163 t
ORIGIN      
        1 aaaaataaga ttttaacgat ggtagcactt actgtcttaa catgtgctac ttattcatca
       61 atcggttatg ctgatacaag tgataagaat actgacacga gtgtcgtgac tacgacctta
      121 tctgaggaga aaagatcaga tgaactagac cagtctagta ctggttcttc ttctgaaaat
      181 gaatcaagtt catcaagtga accagaaaca aatccctcaa ctaatccacc tacaacagaa
      241 ccatcgcaac cctcacctag tgaagagaac aagcctgatg ggagcacgaa gacagaaatt
      301 ggcaataata aggatatttc tagtggaaca aaagtattaa tttcagaaga tagtattaag
      361 aattttagta aagcaagtag tgatcaagaa gaagtggatc gcgatgaatc atcatcttca
      421 aaagcaaatg atgagaaaaa aggccacagt aagcctaaaa aggaacttcc taaaacagga
      481 gatagccact cagatactgt aatagcatct acgggaggga ttattctgtt atcattaagt
      541 ctttacaata agaaaatgaa actttattaa atataaaaag gaggactaac ctccttttta
      601 tatttaaata gcccattcgc catttctaaa gattgggaca gcagttccat ctgcacgaat
      661 accatcaata tccatttgtt ctgaaccaat cataaaatca acatgtgttg aagaacgatt
      721 taaaccagct tcatccaatt cttgacttgt catttctgtt cctccttcta cattgaaggc
      781 atatgcagat ccaatagcga ggtgattaga ggcattttca tcgaataaag tattaaagaa
      841 aattagtcca gacagtgaaa ttggtgtttt atgtgggaca agtgcaactt ccccaagcga
      901 tcttgcccca tcattttcct cgatcaagcg ttggattgtt tcttgccctt tttctgcagt
      961 gacattgata attttaccgt ctttaaaagt aaacgtcata ttttcgataa taacgccagc
     1021 ataactgaga ggttttgtac ttgtcacata cccatctgca cgacggtaat caggtgctga
     1081 aaagatttct tcagtaggca tattagcgat aaaagtctct ccctgagcgt tgagactacc
     1141 ggctgcctcc caaatgtgat ttttaggcat accaagcgtt aaatctgtac ctggtgcggt
     1201 ataatgaaga gcatcaaatt gttcttgatt tagtctagta gctttttcta ataatttagc
     1261 ttcatgtgca tcccaagcag caataggatt atcttcataa atacgattta atttgaaaat
     1321 ggtatcccaa agagcatcaa cttgttggtc gcttgttttt aattctggga agacaagtgc
     1381 agcccattct ctaccagcag ctgcagccac gttccaagat actttattag cttgtattgc
     1441 tctactttgt tcctctaagg caatagcggt tgctctagtt gttttagata aacgtttagg
     1501 atcaacagta gcgaaagcgt tagggttaga agatttcaca actaatcgac tagccttctt
     1561 atttaatagc gctaaagatt tatcaacggt atactgtgga acattcgtga gaacttcgtc
     1621 tgaagcatga agtagtcgct ggcgcgtgat aaaatcatct gtataatcaa caataacttc
     1681 acttgctccc ttttcataag cgacttcagt taaaagcctt gctaagtgga cttgttccac
     1741 atcgattgtt aaagcgagag tgtgcccttt ttggacattt aaacctttag aaataattaa
     1801 ttgggcatat ttttttaaaa ggttgtcgaa atcttgtaaa accatactag ctcctttaaa
     1861 tttactattt tttataattt atcataaact cttataaagc acaaggaaat atggaagcga
     1921 tttaataaag atatatttaa gctaacggtt tgtagtttgc gaaataagac gtttcgtgtt
     1981 aatatagact taactatata ttcagtaaat gaggtgacat gagtggcttt tggagataac
     2041 ggacaacgaa aaaaaactgg gtttgaaaaa ctaactcttt ttgttgttat tttgatggta
     2101 ctagtaacag ttggtggttt ggtatttggt gctattagtg caatcatgta atgagaaacg
     2161 ctgtaaagcg tttttttata ctgaatagaa taaagaatag gaattttaga aatgagtatg
     2221 tttttagata ctgccaaaat tagtgttaag gcaggtcgcg gtggtgatgg catggttgct
     2281 tttcgccgtg agaaatatgt ccccaatgga ggcccgtggg gcggtgatgg cggtaaaggc
     2341 ggatcggtca tttttaaagt aaacgaaggt ttacgaactt tgatggattt tcgatataac
     2401 cgtaatttta aagcaaaagc tggtgaaaaa gggatgacta aaggtatgca cggtcgaggt
     2461 gcagaagacc ttattgtgtc attaccacct ggaacaactg ttcgtgatgc taatacaggt
     2521 aaggttatta ccgaccttgt ggagcatgat caggaattcg ttgtagccag aggtggccgc
     2581 ggtggtcgtg gaaatattcg ctttgcaacc ccacgtaatc cagctcctga aattgctgaa
     2641 aatggggagc ctggcgaaga acgtgaatta caattagaat taaaaatttt ggctgatgtt
     2701 ggtctcgttg gtttcccatc agttgggaag tcgacgctac ttagtgttgt ttcagcagca
     2761 aagcctaaaa ttggtgccta tcattttacg acaattgtcc ctaatttagg gatggttcgt
     2821 acaaaatcag gtgatagctt tgcaatggct gatctccctg gtttgattga aggggctagt
     2881 caaggtgtcg gattaggaac tcagttcctt agacatattg agcgtacacg tgttattcta
     2941 catgttattg atatgtcggc aagtgaagga cgagaccctt acgatgacta tatttcaatt
     3001 aataatgagt tggaaactta caatcttcga ttgatggaac gtccgcaaat tattgttgcc
     3061 aataaaatgg atatgcctga ttcagaagag aatttggcag cattcaaaga aaaattagct
     3121 gcaaactatg atgagtttga tgacatgcct atgatttttc caatttcaag tttagcacat
     3181 caaggactag agaaccttat ggatgcgacg gctaaattat tagccaatac agaagagttt
     3241 cttctttatg acgaaactga catgcaagaa gatgaagcat actatggatt taacgaagat
     3301 gaacgtccat ttgagattac acgtgatgat gatgcaacgt gggtattgta tggtgataag
     3361 ctagaaaaac tctttgttat gacaaatatg gaacgcgatg aatcaataat gaaatttgct
     3421 cgtcagttac gtggtatggg cgttgatgaa gcacttcgag agcgcggagc taaggatgga
     3481 gatattgttc gtataggtaa cttcgaattt gaatttgtag attaatgcag tacttgattg
     3541 gaggtttttt atgggagata aaccgatatc atttcgtgat aaagatggga actttgtttc
     3601 agcggccgat gtctggaatg ctgaaaaatt agaagaactc tttaacacat taaatcctaa
     3661 tcgtaaactt cgtttagaac gcgaaaaatt agcaaaagaa aaaggatagt taagtaacta
     3721 tcctttttag ttattttggg tatgaaaaaa gactctaaaa cataattgat agagtgttaa
     3781 ggtacagatt actttattat tttaa
//
LOCUS       DATROD010000134         3626 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.136, whole genome shotgun sequence.
ACCESSION   DATROD010000134 DATROD010000000
VERSION     DATROD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.136"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            289..1536
                     /locus_tag="VBC74_001856"
     CDS             289..1536
                     /locus_tag="VBC74_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6942769.1"
                     /translation="MSPLNINKNNWRALIAAIVASGTDDLNIMFLAFSMSTIITDLHL
                     SAAQAGWIGTITNLGMLVGGLIFGLLADRYNKFKVFKWTILIFSIATGLVFFTTNLSY
                     LYIMRFIAGIGVGGEYGIAIAIMAGIVPANKMGRISSLNGIAGQVGSISSALLAGWLA
                     PALGWRGLFLFGLLPIVLVLWMQFAIDDKDILDQYNTDADDEPLDISIKALFDTPVLA
                     TQSLALMVMTTVQIAGYFGMMNWLPTIIQTNLNVSVKNSSLWMIATIFGMCLGMLVFG
                     QLLDKFGPRLVYGCFLLSSAICVYLFQFATTMPSMIIGGAVVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNLILNVGRAIGGFSSVIIGMILDVSNVSMVMLFLASLYIVSFLSML
                     SIKQLKRQKYHTNLTQLDVKPTD"
     gene            complement(1579..2424)
                     /locus_tag="VBC74_001857"
     CDS             complement(1579..2424)
                     /locus_tag="VBC74_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HEO6942770.1"
                     /translation="MLSVGLVLEGGGMRGLYTAGVLDAFLDAGIKIDGIVSVSAGALF
                     GVNFVSRQRERALRYNKKYLSHPEYMSLRSWLRTGNFVNKDFTYYEVPMKLDVFDDEA
                     FKKSSIDFYVVATEMTSGKPEYFKIDSVFEQMEILRASSALPVVSKMVDWQGKKYLDG
                     GLSDSIPVDFARGLGFDKLIVVMTRPLNYQKKPSSGRLYKTLYRKYPNFVKTASNRYQ
                     QYNNSLEKVMSLEKTGDLFAIRPSKSLVIGRLEKNPDKLDSIYQLGMKYAKSVMPELN
                     SYLMK"
     gene            2629..>3626
                     /locus_tag="VBC74_001858"
     CDS             2629..>3626
                     /locus_tag="VBC74_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEO6942771.1"
                     /translation="MTYQKTVVLAGDYSYIRQIETTLKSLCVYHENLSIFIFNQDIPQ
                     EWFLAMKDRVGQTGNQIQDVKLFHDHLSPKWENKKLNHINYMTYARYFIPQYISADTV
                     LYLDSDLVVTTNLDNLFQISLDNAYLAAVPALFGLGYGFNAGVMVINNQRWRQENMTI
                     KLIEKNQKEIENANEGDQTILNRMFENQVIYLDDTYNFQIGFDMGAAIDGHKFIFDIP
                     ITPLPKIIHYISGIKPWQTLSNMRLREVWWHYNLLEWSSIISSKKVFGLDHPIKTQNY
                     RLNFLIVTTSDCIPSISELVTALPDCLFHIAAPTVMSDILISLLAFENIRLHKTYTP"
BASE COUNT         1131 a          704 c          564 g         1227 t
ORIGIN      
        1 ttctctcccc acttatcctt gacaataaac taaacttact ctatactatt tccaaatgac
       61 ataatgcttt aaccgtgtcc tgtgagacat ataagcgacc aaaataggat tctatacaga
      121 aatcctacta tttttgcgag cgtttataga actcaagggt agcagcttga gttttttctt
      181 ttacattata atgagttgaa gcagctatga catctctgct ttagaatttt ttctattgac
      241 agtatttaat aacggtggtc tgtgagccat cattaaagga ggagacttat gtcaccatta
      301 aatatcaata agaataattg gcgcgcccta atcgctgcca ttgtcgcatc cggaaccgat
      361 gatttaaata tcatgttcct tgcattttct atgtcaacta tcattactga tcttcattta
      421 tctgcggcac aggctggttg gattgggaca attaccaact tgggaatgct cgtgggagga
      481 ctaatctttg gtttattagc tgatcgctac aataaattca aagttttcaa atggacgatt
      541 ctaatctttt caatcgcaac tggtttagtt ttcttcacta ctaatttatc atatctctat
      601 atcatgcgct tcattgcagg aattggcgtt ggtggcgaat atggtatcgc tattgccatt
      661 atggcaggaa ttgtcccagc taataaaatg ggccggattt cttcactaaa tggtatcgct
      721 ggtcaagtcg gatctattag ctcagcactc ttagcaggat ggctagcacc agcactcggt
      781 tggcggggac tttttctctt tggccttctt cctatagttc tcgtcctatg gatgcaattt
      841 gctattgatg ataaagatat cttagatcaa tataatacag acgcagatga tgaacctcta
      901 gatatcagta taaaagcttt atttgatact cctgtattag ccacacagag cctagcacta
      961 atggttatga caactgttca gattgccgga tactttggca tgatgaactg gttaccaact
     1021 attatccaaa ccaacttaaa cgtttctgtc aagaattcat cattatggat gattgcaacc
     1081 atttttggaa tgtgtcttgg catgctagta tttggtcaat tgttagataa atttggaccg
     1141 cgtttagtat acgggtgttt ccttctatca tctgcaattt gtgtctatct ctttcaattt
     1201 gcaacaacaa tgccttctat gattataggc ggggcagttg tcggcttctt tgttaatggt
     1261 atgtttgcag gctatggtgc catgattaca cgtctctatc cacatcatat tcgttcaaca
     1321 gctaacaatc ttatcttaaa tgttggtcgt gcaataggtg gcttttcgtc tgttatcatc
     1381 ggaatgattc tagacgtttc aaatgtctct atggtcatgc ttttcttagc aagtctctat
     1441 atcgttagtt tcttatcaat gctaagcatt aagcaattaa aacgtcaaaa atatcacact
     1501 aatttaacac aattagatgt caaaccaact gactgatatt acaaaacagt attcctcaat
     1561 tgagaaatac tgttttcttt atttcattag ataactattc agctcaggca tcacactttt
     1621 agcatatttc ataccaagct gataaatact atcaagttta tccggattct tctctaagcg
     1681 gccaataacc aagctcttac tcggtctaat tgcaaataga tcgcctgttt tttcaaggct
     1741 catgaccttt tcaagactat tattatactg ttggtaccga ttcgaggctg tctttacaaa
     1801 attaggatat ttcctataca gagttttata caatcgtcca cttgaaggct ttttctgata
     1861 attgagcggc ctagtcatca caacaatcaa cttgtcaaat cctaaaccac gggcaaaatc
     1921 aacgggaata ctatcagata aaccaccatc taagtacttt ttcccctgcc aatcaaccat
     1981 ctttgagact actggtaatg ctgaactagc acgtaaaatt tccatttgtt caaaaacact
     2041 atcaatttta aaatattcag gtttaccaga tgtcatctct gtagcaacta cgtaaaaatc
     2101 aatacttgat tttttaaatg cttcatcgtc aaatacatcc aatttcatag gaacttcata
     2161 ataggtgaaa tctttattaa caaaattccc tgttcgaagc catgatctta gactcatata
     2221 ttcagggtgg gataaatact ttttattgta tcgcaaagcc ctctctcgtt gtctagatac
     2281 aaaattaaca ccaaacaatg cacccgcaga gacagatacg ataccatcta tttttattcc
     2341 tgcatctaga aaagcatcta aaactccagc agtataaaga cctctcattc cgccaccctc
     2401 taaaactaaa ccaacagaca acataggcaa tcactttcta atattgatat ttgattaaag
     2461 tatagcaaaa tatagctgtt ttttctgaaa aagaatatgt tgtactatat aaactccata
     2521 attaatttaa taataatttc tgaatcacac tcacctatga tagaataata ctaatagttt
     2581 ttaaatttat atttactata tctaactaat ataaataaag gattttttat gacttatcaa
     2641 aaaacagttg ttttggctgg tgattattcc tacattagac aaattgaaac cacattaaaa
     2701 tctctctgtg tctatcatga gaatctctca atttttattt ttaatcaaga tattcctcaa
     2761 gaatggtttt tagctatgaa agatagggtt ggacaaactg gaaatcaaat tcaggatgta
     2821 aagctcttcc atgatcactt atccccaaaa tgggaaaata aaaagcttaa tcatattaat
     2881 tatatgacct atgctcgtta tttcatacct cagtacatct cagctgatac agttttatat
     2941 cttgactctg acttagttgt tactactaat ttagataacc tctttcaaat ttcactagac
     3001 aatgcatatt tagctgcagt tccagctctt tttgggcttg gatatgggtt taatgctgga
     3061 gtaatggtaa ttaacaacca acgttggcga caagaaaata tgactattaa attaattgaa
     3121 aaaaatcaaa aggaaattga gaatgccaac gaaggggatc aaacaattct taatcgcatg
     3181 tttgaaaatc aggtaattta tttagatgat acctacaatt ttcaaattgg ttttgatatg
     3241 ggagctgcta tcgatgggca taaatttatt tttgacatcc caattacccc actcccaaaa
     3301 attattcact acatttcggg aatcaaacct tggcaaacat tatcaaatat gagactccgt
     3361 gaggtatggt ggcactataa tttacttgaa tggtcaagta tcatatctag taaaaaagta
     3421 tttggtttag accacccaat taaaacacaa aattatcgtc tcaatttcct tattgttaca
     3481 acttctgatt gtataccatc tatctcagaa ttagtcactg cccttccaga ttgtctattt
     3541 cacattgcag caccaacagt tatgtctgac attcttataa gcctattagc ttttgaaaat
     3601 atccgccttc ataaaactta cactcc
//
LOCUS       DATROD010000135         3611 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.137, whole genome shotgun sequence.
ACCESSION   DATROD010000135 DATROD010000000
VERSION     DATROD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.137"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..2522
                     /locus_tag="VBC74_001859"
     CDS             <1..2522
                     /locus_tag="VBC74_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEO6942772.1"
                     /translation="FIIIVCILFTKNIYWGSPTTILASDSFHQYVIFNQALRNILHGS
                     NSLFYTFTSGLGLNFYALSSYYLGSFLSPIVYFFNLKNMPDAIYLLTICKVGLIGLSM
                     FVTLCKRHCKVNRVLLLVISTCYSLMSFSISQIEINMWLDVFILIPLVVLGVDQLLWE
                     RKPILHFLSLTALFIQNYYFGFMTTIFTSLYFIVQITRNTDSKVAFKQFLHFTFLSLL
                     AGMTSSIMILPTYFDLTTHGEKLTKVSKMFTENSWYMDLFAKNMIGAYDTTKFGSIPM
                     IYVGLLPLLLSLLYFTIKEIPRRTRLAYGFSLIFVIASFYITPLDLFWQGMHAPNMFL
                     HRYSWVLSVLICLLAAECLEYLDNISWKKILGVNLILISGFIITFLFKKHYHYLNLEL
                     LLLTLTFLSAYIILTISFVSKQIPKLVFYPFLIGFVVLEMTLNTFYQLNSLNDEWVFP
                     SRQGYAKYNHSISKLVRKTERNNSTFFRTERWLGQTGNDSMKYNYNGISQFSSIRNRS
                     SSQVLDRLGFKSDGTNLNLRYQNNTLIADSLFGVKYNLTEYPFDKFGFIRKAQDKQTI
                     LYENQFASQLAILTNQVYQDKPFTVNTLDNQTTLLNQLSGLKETYFEHLIPNSVSGQT
                     TLNKQVFVRKNKQGNTEITYNITIPKNSQLYVSMPFINFNNEENKIVQISVNNGPFVP
                     NTLDNAYSFFNIGSFAENSRIKVKFQFPHNDQVSFPIPHFYGLKLEAYQKAMTVINKR
                     KVKVRTDHNKVIANYTSPNRSSLFFTIPYDRGWKAYQNNKGIKIFKAQKGFMKINIPK
                     GKGKVTLIFIPYGFKFGVGLSITGIVLFTVYYLKFGKNKIG"
     gene            complement(2590..2663)
                     /locus_tag="VBC74_001860"
     tRNA            complement(2590..2663)
                     /locus_tag="VBC74_001860"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2627..2629),aa:Asn,seq:gtt)
     gene            complement(2693..2764)
                     /locus_tag="VBC74_001861"
     tRNA            complement(2693..2764)
                     /locus_tag="VBC74_001861"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2729..2731),aa:Glu,seq:ttc)
     gene            2956..3029
                     /locus_tag="VBC74_001862"
     tRNA            2956..3029
                     /locus_tag="VBC74_001862"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2990..2992,aa:Arg,seq:tct)
     gene            complement(3102..3581)
                     /gene="rlmH"
                     /locus_tag="VBC74_001863"
     CDS             complement(3102..3581)
                     /gene="rlmH"
                     /locus_tag="VBC74_001863"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEO6942773.1"
                     /translation="MKLKIITVGKLKEKYLKEGVAEYQKRLNRFSKIETIELADEKTP
                     DKASISENQRILDIEGERILSKIGERDYVIGLAIEGKQLPSESFSHLIDQKMISGYST
                     ITFVIGGSLGLSQKVKKRADYLMSFGLLTLPHQLMKLVLMEQIYRAFMIRQGTPYHK"
BASE COUNT         1180 a          613 c          535 g         1283 t
ORIGIN      
        1 ccttcataat catagtctgt atcctcttta ctaagaatat ttattgggga agtccaacaa
       61 ccatattagc aagcgatagc tttcatcaat atgttatctt taatcaagca ttaagaaata
      121 ttcttcatgg ttcaaacagc ttattctata cctttacaag cggtttaggt ttaaattttt
      181 atgctctgag tagttactat ttgggaagtt ttttatcccc aatagtttat ttctttaacc
      241 taaaaaatat gcctgatgct atctaccttt taacaatatg taaagtaggg ttgataggtt
      301 tgtcaatgtt tgtcactctt tgtaaacgtc attgtaaagt taatcgtgtc ttacttttag
      361 taatttctac ttgctatagc ttaatgagtt tctcaattag ccaaattgaa attaatatgt
      421 ggctagatgt atttattcta attccccttg ttgttttagg agttgatcag ctactatggg
      481 aaagaaaacc tatacttcat tttctctctc tgacagcctt attcatccaa aactattatt
      541 ttggttttat gactaccatc tttactagcc tttacttcat cgtacaaata acccgtaata
      601 ctgatagtaa ggttgcattt aaacaatttc ttcattttac ctttttatct cttttagctg
      661 gtatgactag tagcattatg attttgccaa cttattttga tttaactact catggtgaaa
      721 agttgacaaa ggtatcgaag atgtttacag aaaattcttg gtacatggat ttgtttgcta
      781 aaaatatgat tggagcctat gatacaacaa aatttggctc tattcctatg atttatgtag
      841 gtttacttcc tttactcctt tctttacttt actttacaat aaaggaaatt cctcgaagga
      901 ctagattagc atatggattt tcactaatat ttgttattgc tagtttttat ataacacctc
      961 tagatttatt ttggcaaggg atgcatgctc caaatatgtt tcttcatcgc tactcttggg
     1021 tactttcagt acttatctgt ctactagctg ctgaatgcct tgagtattta gacaacattt
     1081 cttggaaaaa gattttaggg gtcaatttaa ttttaataag tggtttcata atcacgtttt
     1141 tatttaaaaa gcactatcat taccttaatc ttgaattact tcttttaacc ttaacattcc
     1201 tctcagctta tattattcta actattagtt ttgtcagtaa gcaaatccct aaattagttt
     1261 tttatccctt tcttattggt tttgttgtgt tagagatgac attaaacacc ttttaccaac
     1321 taaatagtct taacgatgag tgggtatttc catctaggca aggatatgct aaatataatc
     1381 acagcattag caaacttgtc agaaagactg agagaaataa ctctacattc ttccgtacag
     1441 agcgctggtt aggacaaacc ggcaacgact ctatgaaata taattataat ggtatttcac
     1501 aattttcatc tattagaaat cgttcttcaa gtcaagtgct tgatagactt ggctttaaat
     1561 cagatggtac aaatttaaat ttacgttatc aaaataatac tttgatcgca gatagtctat
     1621 ttggtgtaaa gtacaacctt acagaatatc cttttgataa atttggtttt atcagaaaag
     1681 ctcaagataa acaaaccatt ctttacgaaa atcagtttgc cagtcaatta gctatactaa
     1741 cgaatcaagt atatcaagat aaaccattta ctgtgaatac tttggataat cagacaactc
     1801 ttttaaatca attaagtggt ttgaaagaga cgtattttga gcatcttatt ccaaatagcg
     1861 tttctgggca aactactctt aataaacaag tatttgttag aaagaataaa caagggaata
     1921 ctgaaattac ctataacata actatcccta aaaacagtca gttatatgtt agtatgccgt
     1981 ttataaattt taataatgag gaaaataaaa ttgttcaaat atccgttaat aatggaccat
     2041 ttgtacctaa tactcttgat aatgcttatt cattttttaa tattggttcc tttgctgaaa
     2101 acagtcgtat caaagtaaaa tttcaatttc cacacaatga tcaagtttct ttcccaattc
     2161 cccactttta tgggctcaag ttagaagctt atcaaaaagc tatgactgtt attaataaac
     2221 gaaaagtaaa agtaagaact gatcacaata aagttattgc taattataca agtcctaata
     2281 gaagttctct tttcttcact attccctatg atagaggttg gaaagcttat caaaataata
     2341 aaggaataaa aattttcaaa gctcagaaag gttttatgaa aataaatatt cctaaaggaa
     2401 aaggcaaagt aacattaatt tttatccctt acggctttaa atttggggta ggcctatcta
     2461 ttactggaat agtattattt actgtctatt atttaaaatt tggaaaaaat aaaataggat
     2521 gagagtactc tcatcctatt ttattaacaa aaaaacgccg acaaatcaac gtttagatgt
     2581 gtcttatcac tccggcagca ggactcgaac ctgcgacatc atgattaaca gtcatgcgct
     2641 actaccaact gagctatgcc ggataaataa aaaactgcaa agcagttatc aatagtccgt
     2701 acgggattcg aacccgtgtt accgccgtga aaaggcggtg tcttaacccc ttgaccaacg
     2761 gaccataggt taggtgtttc cttagtactc ttaatattat atcagaaaaa aaaggtttgt
     2821 ctatactttt gttcaataat ttttacaaaa aaattgaacc tacttatgag caaaaaaata
     2881 tatttccaac tcataaatag tacatatgaa aacttgacag cctgcctaat attttggtag
     2941 aataacagag ttgatggttc catagctcag ctggatagag cattcgcctt ctaagcgaac
     3001 ggtcgcaggt tcgaatcctg ctggaatcag ataatgaaag cctagataga ttatctaggc
     3061 ttttttattt gtttttagcg gttgtttcat aaatggaacg attatttgtg gtaaggagtg
     3121 ccttgacgaa tcataaaagc acgataaatt tgttccatca agactaattt cataagttga
     3181 tgaggtaagg ttaacagtcc aaaactcatt aaataatcag cacgtttttt cactttttga
     3241 gataaaccca aactaccacc aataacaaaa gtaatagtag agtagccgga tatcattttt
     3301 tgatcgatta gatggctaaa tgattcagat ggcaattgtt tcccttctat agccaaacca
     3361 atgacataat ccctctcacc tattttactc aaaatacgtt ccccttctat atctaaaatt
     3421 ctttgatttt cagatatact agctttatca ggtgtctttt catcagctag ctcaattgtc
     3481 tcaattttag aaaagcgatt cagacgtttc tgatactctg caacaccttc tttaaggtat
     3541 ttctctttca attttccaac tgtaattatt tttaatttca tagctacatt ataacataat
     3601 ccacaatatt t
//
LOCUS       DATROD010000136         3584 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.138, whole genome shotgun sequence.
ACCESSION   DATROD010000136 DATROD010000000
VERSION     DATROD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.138"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            1..1142
                     /locus_tag="VBC74_001864"
                     /pseudo
     CDS             1..1142
                     /locus_tag="VBC74_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
     gene            1173..1352
                     /locus_tag="VBC74_001865"
                     /pseudo
     CDS             1173..1352
                     /locus_tag="VBC74_001865"
                     /inference="COORDINATES: protein motif:HMM:NF018196.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
     gene            1356..1694
                     /locus_tag="VBC74_001866"
                     /pseudo
     CDS             1356..1694
                     /locus_tag="VBC74_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            1900..2439
                     /gene="nusG"
                     /locus_tag="VBC74_001867"
     CDS             1900..2439
                     /gene="nusG"
                     /locus_tag="VBC74_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEO6942774.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGKTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRSILISMGQTVDVFDTNIKEGDVVQIIDGAFIGQEGRVVEIENNKVKLMI
                     NMFGSETQAELELYQVAEL"
     gene            complement(2486..>3584)
                     /locus_tag="VBC74_001868"
     CDS             complement(2486..>3584)
                     /locus_tag="VBC74_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 8 glycosyl transferase"
                     /protein_id="HEO6942775.1"
                     /translation="NQDTPVEWVRNINRKLEPLGSKLINVKIYNYDIAHLTTFLTVST
                     WFRLFLADYIPSSRVLYLDSDIIVNTNLDYLFELDFKGHYLAAVKDPHKNEEGGFNAG
                     MLLANLELWREDGLTKTLLKTAEELHRVVKTGDQSILNIVCHNRWLSLNKTWNFQTYD
                     VVSRYNHRSYLYLNIENRTPNIIHFLTSDKPWNENSVARFRELWWYYFQLDFCQLTGK
                     QRKVISYEKSMELLSVSDIHLFTLTSSDNLEHIESLICRCPTVQFHIGAYTTVSNKLS
                     KLEQYPNVLVYPELIEARIEKLITLATAYLDINHGPEVGNILQRVHLKQKPIYSFNNT
                     SHQENITKHIVNHNSIDNMVVLINELNLNQL"
BASE COUNT         1283 a          564 c          672 g         1065 t
ORIGIN      
        1 ttttaagaaa aacagcttat ggtttggctt caatgtcagc ggcgtttata gtatgtagtg
       61 gtattgtaaa tactcctaca gtgtctgctg atagtcctga tacattaaaa gtcgaaaaat
      121 taggcaaatt gaaagatgtg aaatcagttc atgaactcac acccatatca ataccgaacg
      181 aattaaaagg tgctaaagag caagcacttt cttcaataat ttcacatcct aatataacta
      241 attcggaagt agacaaacta gctagtgact atagttttag aattaataca tctaatgatg
      301 tgaacgacgt taaacgtcta ttaaatgaat tttataacgc agttgcaagg aaacagttag
      361 atacaaattc tgctgactac cgtagtaaaa ttgataatat cagtactaca ggtcttgcga
      421 tagctcttga ggctaaagaa atttatgaag caaataaatc tatattacct catcgttaca
      481 aagattctgt tggaacttat gtgaacagtt ttgaggaaag acgaagtcca ggaaaattta
      541 atatttggaa tggtcaggaa ggatttaatg cagctcaaaa attgttagaa gatgttaaaa
      601 aattattact tgagctacaa aatttaacaa aaaataacaa accaaatatt caagtaccta
      661 aacaagcacc tacagaagct gcaaaaccag ctttgtcacc agaagccttg acaagattga
      721 ctacatggta taatcaagct aaagatctgc ttaaagatga tcaagtaaag gacaaatacg
      781 tagatatact ttcagttcaa aaagctgttg accaagctta tgatcatgtg gaagagggaa
      841 aatttattac cactgatcaa gcaaatcaat tagctaacaa gctacgtgat gctttacaaa
      901 gtttagaatt aaaagataaa aaagtagcca aaccagtagc taaaggtaca tacgatgtta
      961 agtatgtaga cacagaagga aaagaagtag ctaagtcacg tcacttcgaa ggagaagaag
     1021 gcgcagcttt tgtcacttca gcgaaagaag tagcgggtta caaacttgtt agaacggaag
     1081 gcgctgtttc aaatgtcttc acagcaggag cacaagtacg tacatatgtt tacgaaaaag
     1141 tagccaaacc agtagctaaa ggtacatacg atgttaagta tgtagacaca gaaggaaaag
     1201 aagtagctaa gtcgcgtcac ttcgaaggag aagaaggcgc agcttttgtc acttcagcga
     1261 aagaagtagc gggttacaaa cttgttagaa cggaaggtgc tgtttcaaat gtcttcacag
     1321 caggagcaca agtacgtaca tatgtttacg aaaaagttaa accagaagtt aaaccagacg
     1381 ttaagccaga ggccaaacca gaggctaagc cagaagttaa accagacgtt aagccagagg
     1441 ccaaaccaga ggctaagcca gaagttaaat cagacgttaa gccagaggct aagccagaag
     1501 ccaagccagc aaccaaaaaa tcggttaata ctagcggaaa cttggtggct aaaaaagcta
     1561 ttgaaaacaa aaagtatagt aaaaaattac catcaacggg tgaagccgca agtccactct
     1621 tagcaattgt atcactaatt gttatgttaa gtgcaggtct tattacgata gttttaaagc
     1681 ataaaaaaaa ttaaatttat ttttaaacat aaatcgaaag gtctatcctt tcaaaatctt
     1741 aaggcaaacc tcatcagaac tttgcctttt ttatttgaat tttagaatga tttagtcggt
     1801 actttaattg acagcttatt ttattttctt tataatatat attgaaaata gcagtccaat
     1861 aagccgtaaa aggctttttt attaaggaaa ggaaaactta tgttagattc ttttgataag
     1921 ggttggtttg tactccaaac ctactcaggt tatgaaaata aagtaaagga aaatctcttg
     1981 caacgtgcgc aaacttataa tatgttagac aacattttgc gtgtggaaat ccctactcaa
     2041 actgtaaatg ttgagaaaaa tgggaaaaca aaagaaattg aagaaaatcg cttcccaggt
     2101 tatgtacttg tggaaatggt aatgactgat gaagcctggt ttgttgttcg taatactcca
     2161 aacgttactg gatttgttgg atcacacggc aatcgctcta aaccaacacc gttgttagaa
     2221 gaagagattc gttcaatact catctcaatg ggacaaaccg ttgatgtctt tgatactaat
     2281 atcaaggaag gtgatgttgt tcaaatcatt gatggtgctt tcatcggaca agaaggacgt
     2341 gtagttgaga ttgagaataa caaagttaaa ttaatgatca acatgttcgg ctcagaaaca
     2401 caagcagaac ttgagttgta ccaagttgca gaattataaa acttctgaaa aatccatctt
     2461 aaaataaaga tggatttttg ttaatttata attgatttag gtttaattcg ttaattaaaa
     2521 caaccatatt atcaatagaa ttatgattaa cgatatgttt ggttatattc tcttgatgag
     2581 acgtgttgtt gaaagaataa attggctttt gtttcaaatg aactctttgt aaaatattac
     2641 ctacttctgg accatgatta atatcaaggt atgcagtggc taatgttatt aatttttcaa
     2701 ttcttgcctc aattaattca gggtaaacta ggacatttgg atattgttct agtttgctaa
     2761 gtttatttga cactgttgtg taggcaccaa tatggaattg aacagtagga catctacaaa
     2821 ttagcgattc aatgtgttcc aaattatcgg aagatgtaag ggtgaaaaga tgaatatctg
     2881 aaacagaaag caattccatg gacttttcgt aagaaatcac ttttctttgc ttgccggtta
     2941 attggcaaaa atcaagttgg aagtaatacc accatagttc tctaaacctt gcaacgctat
     3001 tttcattcca aggtttgtca ctagttaaaa aatgtataat attaggagtt ctattttcta
     3061 tgtttagata taaataagat cgatgattat agcggctaac gacatcataa gtttgaaagt
     3121 tccatgtttt gttcagagat aaccaacgat tatggcaaac aatgttcaag atactttgat
     3181 cccctgtttt gacaactcgg tggagttctt cagctgtttt tagtaatgtt ttagtgaggc
     3241 catcttcccg ccatagttct agattagcta aaagcatgcc agcattaaac cctccttctt
     3301 catttttatg gggatctttg acggctgcta agtaatgacc tttaaaatct agttcaaata
     3361 agtaatcaag attagtgtta acgataatat ctgaatctaa ataaagtaca cgtgaactag
     3421 gtatataatc tgctaaaaat aatctaaacc atgtactaac agttagaaaa gtcgttagat
     3481 gagcaatatc atagttatat attttaacat taatcagttt tgatcctaga ggctctagtt
     3541 tcctgtttat attacgtacc cattcaactg gggtatcttg attg
//
LOCUS       DATROD010000137         3516 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.139, whole genome shotgun sequence.
ACCESSION   DATROD010000137 DATROD010000000
VERSION     DATROD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.139"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            106..636
                     /locus_tag="VBC74_001869"
     CDS             106..636
                     /locus_tag="VBC74_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942776.1"
                     /translation="MSPSEKLDRRIDSSSDLSQYVYDFEKQYTEYMKKGYSEKNASIQ
                     ARRTLNEILQENNPGLRSSDAMYDKETGIAAIAVKDKNTGETYIAYAGTNKDADGATD
                     FISDANIGANNSAYLKRLGDKATSFYDRVQSNGDNITVTTGHSYGDFLASRVAIELIP
                     FFINFRKVNDSFHFCY"
     gene            728..951
                     /locus_tag="VBC74_001870"
                     /pseudo
     CDS             728..951
                     /locus_tag="VBC74_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1283..1759
                     /locus_tag="VBC74_001871"
     CDS             1283..1759
                     /locus_tag="VBC74_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942777.1"
                     /translation="MYIIPAGYSPERLDSEVLTAKIEDDEALLRKIQDLKDHLDKNTK
                     ILAYAFDGLEVRVKARLRKNRKKLKNLIEFNAKSTSVFNELKDYSNAVSSGLTFVSKG
                     FSSFNGQFSSASNLDWQKTIKTGMRKREEDAKQLRKKALEDSNKLRKIASKRGYFE"
     gene            2015..2352
                     /locus_tag="VBC74_001872"
                     /pseudo
     CDS             2015..2352
                     /locus_tag="VBC74_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285002.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2746..3126
                     /locus_tag="VBC74_001873"
     CDS             2746..3126
                     /locus_tag="VBC74_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942778.1"
                     /translation="MVFISDGSPVDANTLYFGKGTPIFMIMNVTIFIISTLIYSYYYL
                     PKNQGKVWKFNQWNNEKSREQQYISNFIVTFGLILLVTPFLKYGIYNSFGVLISLLFT
                     ATLPSLIVDSLYAAFYVHKHPEYE"
BASE COUNT         1281 a          461 c          638 g         1136 t
ORIGIN      
        1 aacggaaagt aaagaatatt attatacaat cagtattgat actgattctg agatatattc
       61 tatattcgaa ggtattacat tagaggatgt aaaggagttt tctaaatgag tccaagtgag
      121 aaattagata gaagaattga ttcttcatca gacctctccc agtatgtata tgattttgaa
      181 aagcaatata cagaatatat gaagaagggg tattctgaaa aaaatgcgag tatacaagca
      241 cgacgaaccc tgaatgaaat actacaagaa aataatccag gattgagatc tagcgacgcc
      301 atgtatgata aagaaacagg tattgcagct attgctgtaa aagataaaaa tactggtgag
      361 acttatatag cttatgcggg tactaataag gatgctgatg gggcaaccga ctttatttca
      421 gatgcaaata tcggtgctaa taactctgct tatttaaaac gattaggaga caaagctact
      481 agcttttatg atcgtgttca aagtaatggc gataatatta ctgtcacaac aggtcattct
      541 tatggtgact ttctcgcttc tagagtggca attgagctaa ttccattctt tattaacttt
      601 agaaaagtta atgattcatt tcacttttgt tattagcttg tgatcactta taaagtgaca
      661 gctagttcat gaaatttctt aggagttttc tggctttaaa ggtaacctat gatttaaaaa
      721 acatctcatg gaattcaatg cggaatcctc aacagtcttt tgtggactaa cagaagttca
      781 ggatgtcgtt tcggcaggcc ttaactattc tcagaaggtt ttggaaattt caatggtaca
      841 tttgatacct caggtaaggg attagaatgg acaaaaacaa ttcaaacagg tatgaaaaaa
      901 cgagaagaga aaatcaaata ccctcatttt acaattgttg acataaagta attaaacaaa
      961 atccggaaaa tttatattaa gtaatttttc taaaaagtca tataggcata cttttaaagt
     1021 tcctctaaac ttaataagga agaaacgttt tgaaaaaaga taaatatatt gcattgaatt
     1081 ggttgtgtgc gatagccacg ctatactatt aagttattac aaactatgaa ttacacctca
     1141 aatgttggag ataaaaccta tcgtttgggg tgttttttgg taaaatagaa tgaaaagagt
     1201 tactctgtgt cattactaag ttaaaaaatt atactaactt aattattaac gttttattga
     1261 ctgagttact cttttttaaa aaatgtatat cattccagct ggttactcac ctgaacgttt
     1321 agactcagaa gtccttacag caaagattga agatgatgaa gccttattgc ggaaaattca
     1381 agaccttaaa gatcatttag ataaaaatac caaaatcttg gcttacgctt ttgatggctt
     1441 ggaagtcaga gtgaaagcta gactaagaaa aaatcgtaag aaactcaaaa accttataga
     1501 attcaatgcc aaatctacct cagtctttaa tgaattgaag gattattcaa atgctgtatc
     1561 gtctggttta acctttgtat ctaaaggctt tagtagtttc aatggccagt tctcttcagc
     1621 ctcaaatctt gattggcaaa agactattaa gacaggaatg agaaaacgag aagaagatgc
     1681 taaacagttg agaaaaaaag ctttagaaga ttcaaataag ctcagaaaaa ttgcatcaaa
     1741 aaggggttac tttgaataat gcagttgaat ttgctaaaga acagaacaaa cccctaaaaa
     1801 taaaaagata accatagata aatatggtcg tgtaaaaatg gataaaactt ttctttatcg
     1861 aaaagctaaa gatggaaaat ctgcatatat ttattctgaa gggagtgatc aatataaaga
     1921 actaactggg aaagaatttg atcctaaaac tacaaatatt atgaaaggta gaaaattagg
     1981 gacagatggt aagatatcaa ttgattttgg tgagattagt aactcaggaa aaaatgcagc
     2041 tattaagtcc cttaaaaatg aaagtccttg aattaaatcc caaaaatgct tttaaaggat
     2101 ggaatgagtt aagcaaaata gctaaaactg ggaaagtact aggatggaca ggtaaaattg
     2161 ctaatattta catgaatgga aaaaaagatc tttttgatga taaaacatct tcaatagatc
     2221 aaaaaatcaa aaactttgct ataggttcag gtgtagatgc attgtccggt gcagcaatag
     2281 ggactttggt aggaggacct gtgggaaccg ttgttggagc aggtgcaggg ctggcatttg
     2341 attttgcttt gaattttaaa gttaaggagt tgggtaatga atcaattaca ggttttgcta
     2401 aaaaaggttt aaaaaatctt gcaggaggat taggattttg aaaaaaagta taaatgtaag
     2461 taggtatcat ggacctttaa ttgaatttga ggttgatgca agttatttaa gaattttttc
     2521 atatatcggt ttacctttat cattgttttg gtattttgtt actcataaca gtgatgactt
     2581 tggaatatta aaatcgatat ttttaatttg tattatagtc aatttaatcg ggctcatatt
     2641 aggattaata caaaagttta ctaaaaaact tatcaaatct ctatcatttt ttgtactttc
     2701 ttagcgtttc tttcgatttt cttagggtta gatacaatat gtttaatggt atttatttcg
     2761 gatgggtccc ctgttgatgc gaatacgttg tattttggta agggtactcc aatttttatg
     2821 ataatgaacg ttacgatatt tatcatttct actttgatct attcctacta ttatttacca
     2881 aaaaatcaag ggaaagtttg gaaatttaat caatggaata acgaaaagag tcgagaacaa
     2941 caatacataa gtaattttat tgttacattt ggtctgatat tgttagttac tccattttta
     3001 aagtatggta tctataacag ttttggagtt ttaatcagtt tactatttac agcaacctta
     3061 ccatcattaa ttgttgattc actttatgcg gcattttatg tccataagca tcctgagtat
     3121 gaataatcaa cttataaaga acatcgaaaa cacatgatac cttttataag acagaaacta
     3181 taaattcaga taaaacatct tcgcttgata agtcagagac tttaaagata gaggataaca
     3241 agctatttta gacaaaaaat tagaaatttc acaatagatt ctagagtaga tactttatta
     3301 ggtgctggaa ggttattggg cacttgattg tgattggact ggacaaaaca aaccagatca
     3361 atcagtaact ggttttgcaa aaaagaatta aaagatttat ttggagggta aaatttttga
     3421 aacagagcaa tcatagatac catggaccgt taattgagtt tgaaatagaa tattcatata
     3481 ttaaaatttt tccatggatt ggactattta tatctt
//
LOCUS       DATROD010000138         3494 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.140, whole genome shotgun sequence.
ACCESSION   DATROD010000138 DATROD010000000
VERSION     DATROD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.140"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..132
                     /locus_tag="VBC74_001874"
     CDS             <1..132
                     /locus_tag="VBC74_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HEO6942779.1"
                     /translation="DTSSGKEDMRKALQIFKYLGEDSFYNTYNEHYCQKVLGNNKEC"
     gene            281..1102
                     /gene="mutM"
                     /locus_tag="VBC74_001875"
     CDS             281..1102
                     /gene="mutM"
                     /locus_tag="VBC74_001875"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEO6942780.1"
                     /translation="MPELPEVETVRKGLERLVVNQEIASITIKVPKMVKTDLNDFMIS
                     LPGKTIQQVLRRGKYLLFDFGEMVMVSHLRMEGKYLLFPNKVPDNKHFHLYFKLTNGS
                     TLVYQDVRKFGTFELVRKSSLKDYFTQKKLGPEPTADTFQFEPFSKGLANSKKPIKPL
                     LLDQRLVAGLGNIYVDEVLWAAKIHPQRLANQLTESETSLLHKEIIRILTLGIEKGGS
                     TIRTYKNALGEDGTMQKYLQVYGKTGQPCPRCGCLIKKIKVGGRGTHYCPRCQCL"
     gene            1093..1686
                     /locus_tag="VBC74_001876"
     CDS             1093..1686
                     /locus_tag="VBC74_001876"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEO6942781.1"
                     /translation="MLMTKIIGLTGGIASGKSTVTKIIRESGFKVIDADQVVHKLQAK
                     GGKLYQALLEWLGPEILDADGELDRPKLSQMIFANPDNMKTSARLQNSIIRQELACQR
                     DQLKQTEEIFFMDIPLLIEEKYIKWFDEIWLVFVDKEKQLQRLMARNNYSREEAELRL
                     SHQMPLTDKKSFASLIIDNNGDLITLKEQILDALQRL"
     gene            1810..2511
                     /locus_tag="VBC74_001877"
     CDS             1810..2511
                     /locus_tag="VBC74_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6942782.1"
                     /translation="MELVIRDIRKRFQETEVLRGASYRFYSGKITGVLGRNGAGKTTL
                     FNILYGDLAADNGTICLLKDNHEYPLTDKDIGIVYSENYLPEFLTGYEFVKFYMDLHP
                     SDDLMTIDDYLDFMEIGQTERHRIIKGYSDGMKSKLSLICLMISKPKVILLDEPLTAV
                     DVVSSIAIKRLLLELSEDHIIILSTHIMALAEDLCDIVAVLDKGKLQTLDIDRKHEQF
                     EERLLQVLKGDEYDK"
     gene            2736..>3494
                     /locus_tag="VBC74_001878"
     CDS             2736..>3494
                     /locus_tag="VBC74_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001882372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942783.1"
                     /translation="MKQLNILPQEGFTLTLTLMVITSLLIVINFYIKLIEPNDMQQTH
                     MIRLFRIEPSRYFQSAELLEASSELFFRSLSFGIFFHLNHFAFWHGLTFALFLAGSRL
                     GIKVLCLPLYGRGKDQENPESLLNILFYLGIGIGVFLSLFFLLLGKQFSPYPFFSWYT
                     GIAGLIIWLVSYYRLKTYSKLNHLTYLTLTHETMKEKIAKIDNIELLGIEMKDRDINV
                     SELSPLLFENLSGISYLNAIFLKRMSRYLQRKILV"
BASE COUNT         1186 a          506 c          666 g         1136 t
ORIGIN      
        1 gatacttctt caggaaaaga agatatgagg aaagctttac agatttttaa atatttaggt
       61 gaagattcat tttacaatac ttataatgaa cattattgtc aaaaagtact tggcaataat
      121 aaagaatgct aatgagtgca agtttatgag taatttttaa agcttttgtc tcaaaatgat
      181 aataagaaag gaaaatagaa atcgaatggt caggtgacat caagaacgtt tggaaattat
      241 tgctctaagc aatgactatt gatttttaga ctatatattt atgccagaat taccagaagt
      301 agaaaccgtt agaaaaggac tagaaagact tgtagttaat caagaaatag cttctataac
      361 gattaaagtc cctaaaatgg tgaaaactga tttgaatgac tttatgataa gtctaccagg
      421 aaaaacaatt caacaggtat tgagacgagg gaagtatctt ctttttgatt ttggtgaaat
      481 ggttatggta tcacatttac gcatggaagg aaaatatcta ctcttcccaa ataaagtacc
      541 agataacaaa cattttcacc tttatttcaa attgactaat ggcagcacat tggtttacca
      601 agatgtccgt aaatttggga catttgagtt ggttcgtaaa tcatctctca aagattattt
      661 tactcaaaaa aaattaggtc ctgagccaac tgctgacaca tttcaatttg agcctttttc
      721 aaaaggctta gcaaactcca aaaagccaat aaaaccttta ctattagatc agagattagt
      781 agcaggactt ggaaatattt acgtagatga agtcttgtgg gcagcaaaaa ttcacccaca
      841 gcgtttggct aatcaattga ccgaatctga aacaagctta ttacacaagg aaattattcg
      901 cattttaact ttaggcatcg aaaaaggtgg ctccacgatt agaacatata agaatgctct
      961 tggagaggac ggtacgatgc aaaagtattt acaggtctat ggtaaaactg ggcaaccgtg
     1021 tccaaggtgt ggttgtttaa ttaagaagat aaaagtagga gggagaggga cgcattattg
     1081 ccctcggtgt caatgcttat gacaaaaata ataggactga caggagggat agcttctgga
     1141 aagtcaacgg taacaaaaat aatacgagaa tcaggtttta aagtcataga tgcggatcaa
     1201 gtggttcata aattgcaagc taagggtggg aaactttacc aagctttatt agaatggttg
     1261 ggtcccgaga tacttgatgc tgatggtgag ttggatagac caaagctttc tcaaatgatt
     1321 tttgctaatc cagacaatat gaagacatca gctaggctac aaaatagtat cattcgtcaa
     1381 gagttagcat gtcagcgcga ccaattaaaa caaacagaag agatattttt catggatatt
     1441 cctttattga ttgaagaaaa gtatataaaa tggtttgatg agatttggtt ggtatttgtt
     1501 gataaagaaa aacaattaca acgattaatg gcccgtaaca actacagtcg agaagaagca
     1561 gaattacgac tttcacacca aatgccttta acagataaaa aaagtttcgc tagtcttatt
     1621 attgacaata atggtgattt aataacttta aaagagcaaa tattggatgc tcttcaacgt
     1681 ttatagaact actaaatgga taatgtattt atccattttt tgtctttatt acactagttt
     1741 taaaaaactt gcgattaaac cacagtaata gtacaatgat ataaaaggtt tactttcaaa
     1801 ggagttttta tggagttagt aattagagat attcgtaagc ggtttcagga aacagaggtc
     1861 ttgagaggag caagttaccg attttattca ggtaaaataa caggggtctt aggtaggaat
     1921 ggtgctggga aaacaacttt atttaatata ctttatgggg atcttgcagc tgacaacggg
     1981 accatttgtt tattgaagga taatcacgag tatcctctta ccgataagga tattggtatt
     2041 gtttattccg aaaactacct tccagaattt ttaacagggt atgaatttgt aaaattttac
     2101 atggatttac atccttcaga tgatttaatg acaatagatg attatttaga ttttatggaa
     2161 ataggacaaa cagagcgtca tagaattatc aaaggatatt ctgatggaat gaagagtaag
     2221 ctctcattaa tttgcctgat gatttctaag ccaaaagtaa ttttactaga tgagccactg
     2281 actgcagttg atgttgtatc aagtattgca ataaaacgcc ttttgttgga attaagtgag
     2341 gatcatatta ttatattatc aactcatata atggccttag cagaagatct atgtgatatt
     2401 gtggctgtat tagacaaagg aaaactccaa acattagata ttgatcgtaa acatgaacaa
     2461 ttcgaagagc gtcttcttca agtgttgaag ggagatgaat atgacaagta aagcattaat
     2521 atggtctata ttacagattg atagtgctag agcttataat agttttttat attaccttca
     2581 gaaaatcccc tggttgggaa gaaaaatccc taaccgatgg tataaaaggt gtagtactat
     2641 ttattcgtat cattttcttt ttcttaagag gttttggtcg ttccataggt tacgtactat
     2701 attgttatgt tttatcattt tttgttagaa gtggcatgaa gcagttaaat attttaccgc
     2761 aagaaggttt taccctaact ttaacattga tggtgattac ctctctttta attgtcataa
     2821 atttctatat aaaacttata gaacctaatg atatgcaaca aactcatatg attcgccttt
     2881 tcagaattga gccatcgagg tatttccaat ccgctgaact tttagaagcc agtagtgaac
     2941 tattttttag aagtttgagc tttggcatat ttttccattt aaatcacttt gctttttggc
     3001 atggtttgac ttttgcttta tttttagcag gtagtcgcct tggaatcaag gtgctctgtt
     3061 tacctttata tggtagaggt aaagaccaag agaatccaga gtctttacta aatattttat
     3121 tttacttagg gattggtata ggagtcttcc tttctctctt tttccttctt cttggcaaac
     3181 agttctctcc atatccattt ttttcatggt acaccggtat tgcaggactt atcatatggt
     3241 tagttagtta ctatcgatta aaaacttatt ctaaacttaa ccacttgacc taccttaccc
     3301 taacgcatga gacgatgaag gagaaaattg caaagattga taatatagag ctgcttggga
     3361 ttgaaatgaa agatagggat ataaatgtca gtgaactttc tcccctgcta tttgaaaatt
     3421 tatcaggtat ttcatattta aacgctatct ttttaaaacg gatgagtaga tacttgcaaa
     3481 ggaaaatttt ggta
//
LOCUS       DATROD010000139         3447 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.141, whole genome shotgun sequence.
ACCESSION   DATROD010000139 DATROD010000000
VERSION     DATROD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.141"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..72
                     /locus_tag="VBC74_001879"
     CDS             <1..72
                     /locus_tag="VBC74_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEO6942784.1"
                     /translation="NAVNYYLQTNDFQKTMQKYNGLK"
     gene            69..>3447
                     /gene="mfd"
                     /locus_tag="VBC74_001880"
     CDS             69..>3447
                     /gene="mfd"
                     /locus_tag="VBC74_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEO6942785.1"
                     /translation="MNIIELFSQNKVVRTWHSGLVTNSRQLVMGFSGASKAIAIASAY
                     EKLSKKIMVVTATQNDSDKLSSDISSLIGEDNVYQFFADDVPAAEFIFSSLDKSISRL
                     SALRFLKDPEKNGVLITSISGLRLLLPNPEVFSKSQYKFEIGQECYLDKLSKNLVNLG
                     YQKVSQVFSPGEFSQRGDILDIFEMTQEYPYRLEFFGDEIDGIRQFDIDTQKSLKQLE
                     SVQISPADDIILQDADFERAKKKLEGYLVTASEEQRAYLSEVLSTTENHFKHSNIRRF
                     LSIFYEKEWGILDYIPEGTPLFVDDFQKIVDRNAKLDLEIASLLTEDLQQGKSHSSLN
                     YFSDPYKQLRQYQPATFFSNFHKGLGNLKFDKLHHFTQYGMQEFFNQFPLLVDEINRY
                     KKSGATVLLQVDSQKGLNLLQENLKEYGLDLIISDKNDIVPKESQLIVGHLSNGFYFA
                     DEKIVLITEREIYHRRVKRKIRRSNISNAERLKDYNELSVGDYVVHNVHGVGKFLGIE
                     TIEIQGIHRDYLTIQYQNADRISIPVEQIELLTKYVSADGKEPKINTLNDGRFKKAKQ
                     RVAKQVEDIADDLLKLYAERSQLQGFAFSPDDNMQNDFDNDFAYVETEDQLRSIKEIK
                     QDMEGNRPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHFENFKE
                     RFSNYPVTVDVLSRFRSKKEQTDTLKRLSKGQVDIIIGTHRLLSQDVVFSDLGLIVID
                     EEQRFGVKHKEKLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLETNPGLVREAIIREIDRGGQVFYVYNKVDTIEQKVSELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFVENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEISEKRLDAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIATKQGKSLIRQKGNVELALQIDAYLPAEYISDERQK
                     IEIYKRIRELETRADYEALQDELIDRFGEYPDQVAYLLEIGLLKAYLDLAFTELVERK
                     GNEISIIFEKASLKYFLTQDYFEALSKTQLKARISETN"
BASE COUNT         1200 a          499 c          663 g         1085 t
ORIGIN      
        1 aatgctgtca attattattt acagacgaat gattttcaaa aaacaatgca gaaatacaac
       61 ggtttaaaat gaatattata gaattattta gccaaaataa agtggtgcgg acatggcact
      121 ctggattagt aactaatagt cgccaattag ttatggggtt ttcaggagca agtaaagcta
      181 ttgctatagc atcagcctac gagaagttat ctaaaaaaat tatggtagtc actgccactc
      241 aaaacgattc tgataaatta tcaagtgata tatcaagttt aattggagaa gataacgttt
      301 atcaattttt tgctgatgac gttcctgcag cagagtttat tttctcttca ttagacaaat
      361 caatttcacg actcagtgcc ttgaggtttc taaaagatcc agaaaaaaac ggtgttttga
      421 ttactagtat cagtggttta aggttactat taccaaatcc ggaagttttc tcgaaaagtc
      481 aatataagtt tgaaatcggt caagaatgtt atttagataa attgagtaag aacttagtca
      541 atttaggtta tcagaaagtt tctcaagtat ttagtcctgg tgaatttagc caaagaggag
      601 atattttaga tatttttgag atgacacaag aatatcctta tcgcttggaa ttcttcggag
      661 atgaaataga tggcatcagg cagtttgata ttgacactca aaagtctctt aaacaattag
      721 agtcagtaca gatttcgcca gcagatgata ttattttaca agatgcagat ttcgagcgtg
      781 caaagaaaaa acttgagggc tatctagtaa cagctagtga ggaacaacgc gcctatttga
      841 gcgaggtttt atctacaacc gagaatcatt ttaagcactc aaatattcgt cgttttctaa
      901 gtatctttta tgaaaaagaa tggggaatat tagactatat tcctgaggga actccacttt
      961 ttgttgatga ttttcaaaaa attgtagatc gaaatgctaa gttagattta gaaatagcta
     1021 gccttctgac ggaagattta caacaaggca agtctcattc tagtttgaac tatttttcag
     1081 atccttataa gcaattaaga caataccaac cagcaacctt cttttcaaat ttccacaaag
     1141 gattaggaaa tctgaaattt gataaacttc atcattttac tcaatatgga atgcaagaat
     1201 tttttaatca atttccattg ttggtcgatg aaatcaatcg ttataagaaa tctggagcga
     1261 cagtactgtt gcaagtagat tctcaaaaag gtttaaatct ccttcaagaa aacttaaaag
     1321 aatatgggtt agatttaatc atttcagata aaaatgatat cgttccaaag gagtcacaac
     1381 ttattgttgg gcatctttca aatggttttt attttgcaga tgagaaaatt gttctgatta
     1441 ctgaacgtga gatttatcat aggcgtgtta aaaggaaaat tagacgcagt aatattagta
     1501 atgcagaacg tctaaaagat tataatgaac tttcagttgg cgattatgtt gttcataatg
     1561 ttcatggcgt aggaaaattt ttaggtattg aaacaattga gatacaaggt attcatcgtg
     1621 attatctgac tattcaatat caaaatgctg atcggatctc tatccccgta gaacaaattg
     1681 aacttctaac aaaatatgtc tctgcagatg gcaaagagcc taaaattaat acgttaaatg
     1741 atggtcgctt caaaaaagca aaacagagag ttgcaaagca agtagaagat attgctgatg
     1801 atttgttgaa actttatgca gagcgtagtc aattacaagg atttgcattt tctcctgacg
     1861 ataatatgca aaatgacttt gataatgatt ttgcatatgt agagacagag gatcaattgc
     1921 ggtcgataaa agaaattaaa caggatatgg aaggtaatcg tccgatggat cgattattgg
     1981 ttggagacgt tggttttggt aagacagagg ttgctatgcg tgcggctttc aaagctgtca
     2041 atgaccataa acaagttgct gttttagtcc caacaacagt tctagctcag cagcattttg
     2101 aaaattttaa agaacgtttt agtaattatc ctgttacagt tgatgtactt agtcgttttc
     2161 gtagcaaaaa agaacaaaca gatacactaa aaagattatc taagggccaa gttgatatta
     2221 ttattggaac acatcggctt ctctctcaag atgttgtatt ttcggattta gggttaatag
     2281 ttattgatga ggaacagcga tttggtgtaa agcataaaga aaagttaaaa gaattaaaaa
     2341 caaaagttga tgtcttaact ttgacagcga ccccaatacc tagaacatta catatgtcaa
     2401 tgcttggcat tagagactta tcagtcattg aaacacctcc aactaatcgt tatcctgtgc
     2461 agacttacgt tttggagaca aatcctggtt tagttagaga agctattatt cgtgagatag
     2521 acagaggagg acaggttttt tatgtttaca ataaagttga cactattgaa cagaaagttt
     2581 cagaacttca ggagttagta ccagaagcta gtattggttt tgttcatggt caaatgtctg
     2641 aaattcaatt agaaaataca ttaatagact ttatcaatgg agattacgat gttttagttg
     2701 caacgacaat aattgagaca ggagtcgata tttcaaatgt taataccctc tttgttgaaa
     2761 atgcagatca tatgggcttg tcaacccttt atcaattaag aggccgtgtg ggtagaagta
     2821 atcgcattgc ttatgcttac cttatgtaca gacctgataa agttttaaca gaaatttctg
     2881 agaaaagact agatgcaatc aaagggttta cagaattggg atctggtttt aaaatagcga
     2941 tgcgtgattt atctatacgt ggtgcaggaa atattttagg agcttcacaa agtggtttta
     3001 ttgattcggt tggattcgag atgtattcac agcttctaga gcaagcgatt gccaccaaac
     3061 aaggaaaatc attaattcgt caaaaaggta atgttgaatt agccttacaa attgacgcct
     3121 atttaccagc agaatatata tcagatgagc gccaaaaaat agaaatatat aagcgtattc
     3181 gtgaattaga aactagggct gattatgaag ccttacaaga tgagttaata gatcgttttg
     3241 gagaatatcc tgatcaagtt gcataccttt tagaaatagg acttttaaaa gcttatctgg
     3301 atcttgcctt tactgaatta gtagaaagaa aaggtaatga aataagcatc atatttgaaa
     3361 aagcatcttt gaaatatttt ttaactcaag attattttga agcactatca aaaactcaac
     3421 ttaaggctag aattagcgaa acaaatg
//
LOCUS       DATROD010000140         3314 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.142, whole genome shotgun sequence.
ACCESSION   DATROD010000140 DATROD010000000
VERSION     DATROD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.142"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(54..1202)
                     /gene="nagA"
                     /locus_tag="VBC74_001881"
     CDS             complement(54..1202)
                     /gene="nagA"
                     /locus_tag="VBC74_001881"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEO6942786.1"
                     /translation="MTKYIKADRFFYADHVKENGYLEIKDNHFGKWIENISGQEEILD
                     YSGYQIAPGLVDTHIHGFAGADVMDCDSEGILRMSAGLLSTGVTSFLPTTLTSDTKRL
                     EEASKAVAAVAGKEQGAKIQGIYFEGPYFTEEYKGAQNPIYMRNPNLEEFAQWQKAAK
                     GLITKIALAPEREGVEEFVSAITKQGVTVALGHSNGTYKEAKKAVKAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVDPVACDILMTQKGHNHVALITDCMAA
                     GGAPDGDYMLGELPVVVSNGTARLKDGGNLAGSILKLKDGLKNVVEWGIATPEEAVRM
                     ASYVPAVSVGIDNVCGQIKEGYAADFIVLDKHLDLVATYLDGEKRWQS"
     gene            complement(1480..2187)
                     /locus_tag="VBC74_001882"
     CDS             complement(1480..2187)
                     /locus_tag="VBC74_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEO6942787.1"
                     /translation="MKIDKKELLALIASIILLIFASVTFFLFKDHGTTQMDTVESSVN
                     HVSDSQLTEAQDMLDKFEKKPSEKLLKDVELALNKLSNSSKKEALQKRFKKAKDKYLK
                     DEADKKATKDATDLVEILEQAPSEENVLKAEAAVKKLTVKESKEALQKRIDTVKTQYG
                     LIGNQTPSSSVAETTEQGTANPASQDTSSYVNQDVAPTYEQPQTNNTPVTPGVNNTVP
                     TPGTGTVPATNGTGVAQ"
     gene            2378..2689
                     /locus_tag="VBC74_001883"
     CDS             2378..2689
                     /locus_tag="VBC74_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEO6942788.1"
                     /translation="MIPSQMLKGLLEGAILKIIQQEETYAYEISKKLNSYGFGEISEG
                     TIYPIILRLQSNKYIKGQQKPSPQGPKRKYYTLTNEGVEQLAQFKQNWESLSKALNKL
                     L"
     gene            2710..>3314
                     /locus_tag="VBC74_001884"
     CDS             2710..>3314
                     /locus_tag="VBC74_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942789.1"
                     /translation="MDKLKKLQKENTLLEGRIDNSNNQTYTDMIVYLRGASISPYHQE
                     LIRNDIVNMLLEAQERQASLVSVFGEDRHDFINQVIKSTPKISKKEETLQRWDLAILL
                     LTIQMIIFLGGYLITEALQQSVPDLIPITLLDVLFAIFISIIAVKITDTIIYATYNFD
                     KSKEKKYFFRYMFLILSLIIAYILIGKYCHLPFINIPLWIY"
BASE COUNT         1061 a          669 c          487 g         1097 t
ORIGIN      
        1 tatatttcat taaaaattaa taaattaaaa agaactagta aaaactagtt ctttcaagat
       61 tgccaacgtt tttctccatc aaggtatgtt gcaactaaat ctagatgttt atctaataca
      121 ataaaatccg ctgcatagcc ttcttttatc tgtccacaga cattatcaat gcctacagac
      181 actgctggaa cataactcgc catacgcact gcctcttccg gagtagcaat tccccattct
      241 acaacatttt ttaatccatc tttgagctta agaatagaac cagctaaatt ccctccatct
      301 ttcaaacgcg cagttccatt agacacaacc acgggcaatt cccctaacat ataatctcca
      361 tcaggggcac ctccagctgc catacaatct gttattaatg ccacatgatt atggcctttt
      421 tgtgtcatta aaatgtcaca agcaacagga tcaacgtgat gaccatcgca aatgagttca
      481 gcataagtat taggtaaatt ataaaccgca ccaaccatac ctggttctcg gtgtgtcaaa
      541 ccacgcatac cattatacgc atgtacccaa actgaggcac ccgccttcac tgccttttta
      601 gcttctttat atgtcccatt agaatgacct aaagctaccg taacaccttg ctttgtgatt
      661 gctgagacaa attcctcaac tccctcacgt tctggtgcca aagcaatttt tgtaatcaag
      721 ccttttgcag ccttctgcca ttgagcaaac tcttccaagt ttggatttct catataaatc
      781 ggattttgag ctcctttata ttcctcagta aaatatggtc cctcaaaata gatgccttga
      841 atttttgctc cttgttcttt gcccgctact gccgcaactg ctttagacgc ctcttctagt
      901 cgctttgtat ctgaagttag ggttgtcggc aagaaagatg tgactccagt agatagtaag
      961 ccagcactca ttcgcaaaat tccttctgaa tcacaatcca taacatcagc gccagcaaaa
     1021 ccatgaatat gtgtatcaac taaaccaggt gctatttggt aacctgaata atctaaaatc
     1081 tcctcttgtc cagaaatatt ttctatccat ttaccaaagt gattatcttt tatttctaaa
     1141 taaccgtttt cctttacatg atcagcataa aagaatcggt cagccttgat gtacttcgtc
     1201 atcttcttcc ccccattcac tctcattata acgctttaag gtatttttgt aaatggtata
     1261 tacctatttt ttaaaaagtc atggtttatt ctaagaaaga actatacact tgttataaac
     1321 cacttcactt ggttgtactt ttaaggtttt aattgctatt gctcataatt cttttccttc
     1381 tccaaaacta tcaattgcta attctcttat agtttttgat gagaatggct aattaaaaag
     1441 catagcaaaa aagtctagta aaactagact ttcaggattt tactgagcaa cacccgtccc
     1501 attagtagca ggtacagtac cagttcctgg agtcggaaca gtattgttaa cccctggagt
     1561 aactggtgta ttattcgttt gcggttgctc ataagttggt gctacatcct gattaacgta
     1621 actagaagta tcctgactag caggattagc tgtcccttgt tctgtagttt cagctactga
     1681 actagaggga gtttgattcc caatcaaacc atattgtgtc ttaacagtat caatacgttt
     1741 ttgtaaagct tctttagatt ctttgacagt taactttttg actgctgctt cagcttttaa
     1801 cacattttct tctgaagggg cttgttctag aatttctact aaatctgtag catctttggt
     1861 agctttctta tctgcttcgt ctttaagata cttatctttt gctttcttaa aacgtttttg
     1921 caaagcttct tttttagaag aattggacaa tttatttaaa gcaagttcga catccttcaa
     1981 taatttttct gaaggtttct tctcaaattt atctaacata tcttgtgcct cagttaattg
     2041 agaatcactg acatggttaa cactgctttc tacagtatcc atttgagtag tgccatgatc
     2101 tttaaataag aaaaacgtaa cagaagcaaa aattaataaa ataatactag caataagtgc
     2161 taaaagttct tttttatcta ttttcatact cactcctaaa gtttcttcac attattataa
     2221 cataaactaa aggacataaa caaacctatt ttagaagaat catcgtctat cactcttatt
     2281 ttctaccggt aacttagtta tatttgacat ctcaactact ttgtattaca atgtagttaa
     2341 ccagtaactt gtattacaat atagataagg atataatatg attccatcgc aaatgctaaa
     2401 aggattactt gaaggagcta tcctaaaaat catacagcaa gaggagactt atgcttatga
     2461 aatctcaaaa aaattaaatt cttatggttt tggggaaatt tccgaaggga caatctaccc
     2521 cattatctta aggctacaaa gcaataaata tataaaagga caacaaaagc cttcccctca
     2581 aggacctaaa cgtaaatatt atacgttaac caatgaaggc gtggagcaat tagcccaatt
     2641 taaacagaat tgggagtcac ttagtaaagc attaaacaaa ctactctaaa aattggagag
     2701 gaaactagca tggataaact gaaaaaatta caaaaagaaa atacgctatt agaaggaaga
     2761 attgataact ctaataacca aacctataca gatatgattg tctatttgcg aggtgctagc
     2821 attagcccct accaccagga gctcattcga aatgatattg tcaatatgtt attagaagca
     2881 caagagcgac aagcaagttt agttagtgta tttggagaag ataggcacga ctttataaac
     2941 caagttatta aatctacacc taaaatctct aagaaagaag aaacattaca acgttgggat
     3001 ttagccattt tactactaac tatccaaatg attatctttt taggaggata tctcattact
     3061 gaagcacttc aacagtcagt accagatcta atccctatta ctctattaga tgttcttttt
     3121 gcaatcttta tcagtattat tgctgtcaag ataactgata ccataatata tgctacatat
     3181 aacttcgata aatcgaaaga aaagaaatat ttctttcgat atatgtttct aattctttcg
     3241 cttatcatag cttatatcct tataggaaaa tattgtcatc ttcctttcat taatatcccc
     3301 ctttggattt attt
//
LOCUS       DATROD010000141         3197 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.143, whole genome shotgun sequence.
ACCESSION   DATROD010000141 DATROD010000000
VERSION     DATROD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.143"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..956)
                     /gene="tilS"
                     /locus_tag="VBC74_001885"
     CDS             complement(<1..956)
                     /gene="tilS"
                     /locus_tag="VBC74_001885"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEO6942790.1"
                     /translation="MYNTILKDTLSKGLFTAHQKVLIAVSGGIDSINLLQFLYQYQKE
                     LSISIGIAHINHGQRKESEKEEEYIRQWGQIHDVPVFISYFQGIFSEDRARNHRYNFF
                     SKVMREEGYTALVTAHHADDQAETVFMRILRGSRLRYLSGIKQVSAFANGQLIRPFLP
                     YKKELLPNIFHFEDASNASSDYLRNRIRNIYFPTLERENNQLKDSLITLSEETECLFT
                     ALTDLTRSIEVTNCYDFLRQTHSVQEFLLQDYISKFPDLQVSKEQFRVILKLIRTKAN
                     IDYTIKSGYFLHKDYESFHITKIHPKTDSFKVEKRLELHNIQ"
     gene            complement(958..2244)
                     /locus_tag="VBC74_001886"
     CDS             complement(958..2244)
                     /locus_tag="VBC74_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEO6942791.1"
                     /translation="MKKVLTFLLCSLYFVSIPAISTEEPLTLSQNRRYALTQTVVDKE
                     MYFDAIPERPTTKIEISSFQDEALTITGETLVPNTLLSIISLTINSNGIPVFTLSNGQ
                     FIKASREAIFNDLVSKQQSVSLDYWLKPSFVTYEAPYTNGVSEVKNNLKPYSRVHLVE
                     QAETEHGIYYKTDSGFWISVEDLSVADNRMAKVQEVLLEKYNKDKYGIYIKQLNTQTV
                     AGINIDRSMYSASIAKLATLYASQEQVKLGKLSLDSKFEYKDNVNQFPNSYDPSGSGK
                     LEKKADHKLYTVKELLEATAKESDNVATNMLGYYVNNQYDSMFQTQVDTISGMHWDMK
                     KRQISPQAAGKMMEAIYYQNGDIVNYLSKTDFDNTRIPKNIPVKVAHKIGDAYDYKHD
                     AAIVYAEQPFIMIIFTDKSSYDDITKIADDVYQVLK"
     gene            complement(2244..2378)
                     /locus_tag="VBC74_001887"
     CDS             complement(2244..2378)
                     /locus_tag="VBC74_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HEO6942792.1"
                     /translation="MNNLIETIEHFLAYSDDKLEELADKNYQLRQTIVEKEKIESREK
                     "
     gene            complement(2381..2752)
                     /locus_tag="VBC74_001888"
     CDS             complement(2381..2752)
                     /locus_tag="VBC74_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEO6942793.1"
                     /translation="MSKPNVVQLNNQYINDENLKKRYEAEELRRKNRLMGWVLIFVML
                     LFILPTYNLVKSYRTLQERRQEVVKLTKDYQTLTNRTENQKLLAKQLKNPDYVQKYAR
                     AKYYFSKTGEMIYPLPDLLPK"
     gene            complement(2739..3011)
                     /locus_tag="VBC74_001889"
     CDS             complement(2739..3011)
                     /locus_tag="VBC74_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEO6942794.1"
                     /translation="MRLDKYLKVSRIIKRRPVAKEVADKGRVKVNGVLAKSSTDLKLN
                     DQVEIRFGNKLLTVKVLEMKDSTKKEDAIKMYEIINETRIETDEQA"
BASE COUNT          984 a          558 c          457 g         1198 t
ORIGIN      
        1 atttgtatat tatgcaattc taatcttttt tcaaccttaa aactatccgt cttaggatgg
       61 atttttgtaa tatgaaaact ctcgtaatcc ttatgaagaa aatagcccga tttaatagta
      121 tagtcaatat tggctttagt tctaatcaat tttaatatta ctctgaactg ttctttactc
      181 acttgtaaat ctggaaattt gctaatgtaa tcttgcaata aaaattcttg gacggaatga
      241 gtttgcctta aaaaatcata gcagtttgtt acttcgatgc tccttgttag gtctgttaaa
      301 gccgtgaata aacactctgt ttcctcagag agagttatga gagaatcttt caattgatta
      361 ttttctcttt ccaaagtagg aaagtagatg tttcttattc gatttctcaa ataatcagac
      421 gaagcattgc tagcatcttc aaaatgaaaa atatttggta ataactcctt tttatatgga
      481 agaaatggtc taatcaattg accatttgca aaagcactca cttgcttaat ccctgataaa
      541 tagcgtagcc tgcttcctct taaaattctc ataaatacag tttcagcttg atcatcagca
      601 tggtgagctg ttactaaagc cgtgtaacct tcttcacgca taacttttga aaaaaaattg
      661 tagcgatggt tacgtgctct atcttctgaa aatattcctt gaaaataaga gataaagaca
      721 gggacatcat gtatctgtcc ccactgtctg atatattctt cttctttttc tgattctttt
      781 cgctgtccat gatttatgtg tgcaatacca atactaattg ataactcttt ttgatactgg
      841 tataaaaatt gcaacaaatt tattgaatca atgccaccag aaacagcaat tagaactttc
      901 tgatgagcgg taaataaccc tttggataag gtatctttca aaatagtgtt atacatatta
      961 ctttaaaacc tggtaaacgt cgtcagcaat cttagtgata tcatcgtaag atgatttatc
     1021 tgtaaaaata atcataataa aaggttgttc tgcataaaca atagccgcat catgtttgta
     1081 gtcataagca tctccaattt tatgtgctac tttaactggt atatttttag ggattcttgt
     1141 attatcaaaa tcagtctttg ataggtaatt aacaatatct ccattttgat aataaattgc
     1201 ttccatcatt ttacccgcag cttgtggaga aatctggcgt ttcttcatat cccagtgcat
     1261 accagaaatt gtgtctactt gtgtttgaaa cattgaatcg tattgattat tgacataata
     1321 acctagcata tttgttgcaa cattatctga ttccttggca gttgcttcaa gtaactcctt
     1381 aactgtatat aatttatgat cagctttttt ctctaatttc ccactaccac ttggatcata
     1441 gctattagga aattgattaa cattatcttt gtattcaaat ttgctgtcca atgacagttt
     1501 tcctaatttt acttgctctt ggctagcata aagtgttgct aattttgcaa tacttgcaga
     1561 gtacattgac ctatcaatat taataccagc tactgtctgt gtatttaatt gtttgatgta
     1621 tataccatat ttatctttat tatatttttc caataatact tcttgaacct tagccatacg
     1681 attatcagca accgatagat cttctactga tatccaaaaa ccactgtccg ttttataata
     1741 aataccatgt tctgtttctg cttgttctac taaatgcact ctggaataag gttttaaatt
     1801 atttttaact tcactaacac catttgtgta aggtgcttca taagtcacaa aagaaggttt
     1861 taaccaataa tccaatgaga cagattgctg cttagaaact aaatcattaa aaatagcttc
     1921 acgactagcc tttatgaact gtccattgga caaagtaaat actggaattc catttgaatt
     1981 aattgtaagg gaaataatcg acaataaagt atttggaact aaagtctctc ctgttatggt
     2041 taaagcttca tcttgaaaac tgcttatttc aatttttgta gttggtctct ctggaatagc
     2101 atcaaaatac atttccttat ccacaactgt ctgagttaag gcgtatcgtc tattttgaga
     2161 aagcgttaat ggctcctcgg tacttatagc tgggattgat acaaaatata agctacataa
     2221 taagaaggtt aaaactttct tcattacttt tctctgctct caatcttttc tttttctaca
     2281 attgtttgcc taagttgata gttcttatca gctaactctt ctagtttgtc atccgagtag
     2341 gctagaaaat gttctattgt ttcaattaaa ttattcataa ttattttggt aaaaggtctg
     2401 gtaatgggta aatcatttcg ccggtcttag agaaataata cttcgctcga gcatattttt
     2461 gaacgtaatc tggatttttt agttgttttg ctagtaactt ctggttctca gttctattag
     2521 ttaatgtctg atagtctttc gttaatttta caacttcttg acgacgttct tgtaaagttc
     2581 tgtaactctt aactaaatta taagtgggta aaataaataa aagcatgaca aaaataagaa
     2641 cccaacccat taaacgattt tttcggcgta actcctcagc ttcgtaacgt ttttttagat
     2701 tctcatcgtt aatatattga ttatttaact gaacaacatt aggcttgctc atctgtttct
     2761 atccttgttt cattaataat ttcatacatt ttgatagcat cttctttctt agtgctatct
     2821 ttcatttcta atactttaac ggtcaacaat ttattaccaa aacgaatttc aacttggtca
     2881 ttaagcttta ggtcagttga tgactttgct aatacgccat tcaccttgac acgtcctttg
     2941 tctgcaactt ctttagcaac gggacgacgc ttaatgatac gcgacacttt tagatacttg
     3001 tctaatctca ctgatgatca catccttcta tctaaattaa ctctactatt ttagcatttt
     3061 ttttgaaaaa tatctgaact gataatattt gtcaatataa tgtaacaaaa agagagctat
     3121 tagctctctt aattctctca aatatcaagt aaaaaacttc tattttaata acctctaaaa
     3181 atatttatgt ctttcaa
//
LOCUS       DATROD010000142         3191 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.144, whole genome shotgun sequence.
ACCESSION   DATROD010000142 DATROD010000000
VERSION     DATROD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.144"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(30..902)
                     /gene="sdaAA"
                     /locus_tag="VBC74_001890"
     CDS             complement(30..902)
                     /gene="sdaAA"
                     /locus_tag="VBC74_001890"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEO6942795.1"
                     /translation="MFYTIEELVEQANSQHKGNIAELMIQTEIEMTGRSREEIRYIMS
                     RNLEVMKASVIDGLTPSKSISGLTGGDAVKMDQYLQSGKTISDTTILAAVRNAMAVNE
                     LNAKMGLVCATPTAGSAGCLPAVISTAIEKLNLTEEEQLNFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSASAMAAAALVMAAGGTPFQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNALGSSFALVAADMALAGIESQIPVDEVIDAMYQVGSSLPTAFRETAEGGLAA
                     TPTGRRYSKEIFGE"
     gene            complement(917..1585)
                     /gene="sdaAB"
                     /locus_tag="VBC74_001891"
     CDS             complement(917..1585)
                     /gene="sdaAB"
                     /locus_tag="VBC74_001891"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEO6942796.1"
                     /translation="MKHLKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGEPSE
                     VTFHLYNSFAKTYQGHGTDKALVAGILGMDTDNPDIKNSLEIAHQKGIKIYWDILKDS
                     NSPHPNTAKITVKNGDRSMSITGVSIGGGNIQVTELNGFSVSLTMNTPTLIIVHQDIP
                     GMIAKVTDILSDFNINIAQMNVTRESAGEKAIMIIEVDSRDCQQAVKKIEAIPHLHNV
                     NFFD"
     gene            1831..2952
                     /gene="mnmA"
                     /locus_tag="VBC74_001892"
     CDS             1831..2952
                     /gene="mnmA"
                     /locus_tag="VBC74_001892"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEO6942797.1"
                     /translation="MTDNSNIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVTRDENGIVHMLRGADNNKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKPEVRRIAEEAGLATAKKKDSTGICFIGEKNFKDFLGQYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNKPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLTASEIHFTRDMPNEFKLECTAKFRYRQPDSKVTVYVKGNQARVVFD
                     DLQRAITPGQAVVFYNEQECLGGGMIDQAYRDDKICQYI"
     gene            2984..>3191
                     /locus_tag="VBC74_001893"
     CDS             2984..>3191
                     /locus_tag="VBC74_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEO6942798.1"
                     /translation="MISKFILAFMAFFAIMNPISNLPAFMALVADDDQKISRRIAAKG
                     VLLAFVIIVIFVLSGHLLFNLFGIT"
BASE COUNT          996 a          575 c          591 g         1029 t
ORIGIN      
        1 tcagggaaag agtagaaaat tgataaactt tattccccaa aaatttcttt gctataacgt
       61 cttcctgtcg gcgtggcagc aagtcctcct tctgcagttt cacgaaaagc agtcggtaaa
      121 cttgatccaa cttgatacat tgcatcaata acttcatcta ctggaatttg cgattcaata
      181 ccagccaagg ccatatcagc agcaacaagt gcaaaacttg atccaagagc attccgcttc
      241 acacaaggga cttcaactaa acctgcaaca gggtcacaga taagtccaag catattttta
      301 ataacaaatg ctatagcttg gctagcttgg aaaggagtac ctccagcagc cataactaaa
      361 gcagccgcag ccatagcact agctgaccca acttcagctt ggcaacctcc ttctgcacct
      421 gagatagagg cattattacc aatgacaaga ccaaatgcgc cggctgtaaa tagaaaatta
      481 agttgctctt cttctgttaa attaagcttt tcaatggctg tagaaatcac agctggtaaa
      541 catcctgcac tacctgcagt tggtgttgca cagaccagtc ccatcttagc atttaactca
      601 ttaacagcca tagcattcct aacggcagct aggattgtgg tatctgaaat agtttttcct
      661 gattgtaaat attgatccat cttgacagca tcaccgcctg ttaaaccact gattgattta
      721 ctaggggtca atccatcaat aacagaagct ttcataacct caaggtttcg ggacataata
      781 taacgaattt cttcacgact tctaccagtc atttcaattt ccgtttggat catgagctct
      841 gctatgttac ccttatgttg gctattagct tgctctacca gctcttcaat tgtataaaac
      901 atagttaccc tttctcttaa tcaaagaagt tgacattgtg taaatgagga atagcttcta
      961 tctttttgac agcttgttga caatctcgtg aatctacttc tataatcatg atagcttttt
     1021 ctcctgcact ttcgcgtgtt acattcattt gagcaatatt aatgttaaag tcagataaaa
     1081 tatcagttac tttagcaatc atgccaggta tatcttgatg aacgataatt aaagtaggag
     1141 tattcattgt taatgaaact gagaatccat tcaactcagt aacttggatg tttccgccac
     1201 caattgacac tcctgtaata ctcatgctcc tatccccatt tttaacagtt attttcgcag
     1261 tatttggatg tggtgaatta ctatctttta ggatatccca gtaaatctta attccttttt
     1321 gatgagcaat ttcaagagag tttttaatat ctggattatc tgtatccatt cctagaatcc
     1381 ctgcaaccaa tgctttatca gtaccgtgtc cttggtaagt tttagcaaaa gaattgtata
     1441 aatgaaaggt tacttcacta ggttcaccaa aaatagaatg gacaacttta ccaatgcgga
     1501 cagctcctgc agtatgacta cttgatggtc caatcataac aggaccaata atgtcgaaga
     1561 ccgattgaaa ttttaaatgt ttcatagttt ttcctcgtga tttttgtaca ttctcattat
     1621 aacagattat ttctaaaaaa tatcataaaa agataaaaaa actgcaatgt aataaatatt
     1681 tatcacaaca aaaacataca acttgacaac aaatagtgct ataatatata agcactttta
     1741 tagtgttttt tagtcaatat gattgatcta agaacaactt ggcccttcaa gcctaagtta
     1801 aaaataaaac tttttgatgg gagaattctt atgactgata attcaaacat tcgtgtcgtt
     1861 gtaggcatga gtggaggtgt tgattcatca gtaactgccc tgcttttaaa agagcaaggt
     1921 tatgatgtta ttggtgtttt tatgaaaaat tgggatgaca cagatgaatt tggtgtttgt
     1981 acggctactg aagattataa agatgttgct gctgttgctg atcagatcgg cataccatac
     2041 tactctgtca actttgaaaa agagtattgg gacagggttt tcgaatattt tttagcagaa
     2101 tatcgagcag gtcgtacgcc aaatccagat gttatgtgta ataaggaaat taaatttaaa
     2161 gcatttctag attatgcgat gacattaggt gctgactatg tagcaacagg tcattatgca
     2221 caagtaacac gtgatgagaa cggaatagtt catatgctta gaggagcaga taataataag
     2281 gatcaaactt attttctaag tcaattatct caggagcaat tgcaaaaaac gttatttcca
     2341 cttggtcatt tacaaaaacc agaagttcgc cgtatagctg aagaagcagg acttgcgacc
     2401 gctaagaaaa aagactctac tggtatttgt tttattggag aaaaaaattt caaggatttc
     2461 ctgggtcaat atttaccggc tcaaccaggt cgtatgatga ctgttgatgg tcgtgacatg
     2521 ggtgaacatg ctggtttaat gtattatact attggtcaaa gagggggcct tggtattggt
     2581 gggcaacatg gtggagataa taaaccttgg tttgtagttg gaaaggattt aagtaaaaac
     2641 attctttatg taggtcaagg tttctatcat gactcattga tgtcaactag cttgacagct
     2701 tctgaaatcc actttacacg tgatatgcct aatgaattta agctagaatg tacagctaag
     2761 ttcagatatc gtcaacctga cagtaaagtt acagtttacg taaaaggtaa tcaagcaagg
     2821 gttgtgtttg atgatttaca acgagcaatc acaccaggtc aagctgttgt tttttataat
     2881 gaacaagaat gtttaggcgg tgggatgatt gatcaggctt atcgtgatga taaaatctgt
     2941 caatacattt aattgacact taattattaa gtgaggtaat cgtatgattt caaaatttat
     3001 tttagctttc atggcttttt tcgccattat gaatcccatt tctaatttgc cagcttttat
     3061 ggctttagta gctgatgatg atcagaagat ttcacgtcgg attgcagcaa aaggagtatt
     3121 acttgctttt gtcattattg taatctttgt attaagtggc cacttacttt ttaacttatt
     3181 tgggattacc c
//
LOCUS       DATROD010000143         3177 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.145, whole genome shotgun sequence.
ACCESSION   DATROD010000143 DATROD010000000
VERSION     DATROD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.145"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            212..1288
                     /locus_tag="VBC74_001894"
     CDS             212..1288
                     /locus_tag="VBC74_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6942799.1"
                     /translation="MLGENIYLQRTQIGMTQENLSDYLHLTKTTISKWENNQAKPDID
                     YLILMANLFDISLDDLVGYQKTLSDDQRNQLIKDLKIKANVLSERDFFQEVKELSKQF
                     PNDFKTLLIMINMVLSNLTNLNDSEMKEWSLSTLNKIISKTVVESDLQTAIMFKTVVL
                     FHNLEYDEVINLYQNSPYKLGEELLLANSFFAKGDVYRAKQVLQVEIYQQILLVCEYL
                     LSLSTFEVEENKGDIFERLEHMIEAFQLNSLHPNTVIKCYYSLACHYSKSDDDKALGY
                     LKQCFKSLKQLLQRFELHGDTFFYTIDDWLETIPTGIAPPTSQLQVIERVEDLFQNSQ
                     FKELSGRKEFQQIIQKLNDLKKDY"
     gene            1290..1496
                     /locus_tag="VBC74_001895"
     CDS             1290..1496
                     /locus_tag="VBC74_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEO6942800.1"
                     /translation="MNTEIPNQLLPFLVPLIMLQGALIIISLVKLSKLKMTKHLSKPV
                     WFLVIIFLNIIGPIAFLILEGNNE"
     gene            1493..2395
                     /locus_tag="VBC74_001896"
     CDS             1493..2395
                     /locus_tag="VBC74_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6942801.1"
                     /translation="MKAISFQNVTKSFGPKKILNNVSFDLEENMIYGFVGPNGAGKTT
                     TIKMILGLLKFDTGFITIFGKKVNFGRTDTNQLIGYLPDVPEYYDYMTALEYLDLCSG
                     LARSKHKLSNKELLRSVGLDDNHQKIATYSRGMKQRLGLAQALVHDPKIIICDEPTSA
                     LDPKGRQDILDIISNLRGEKTVIFSTHILSDVEKICDHVLVLTKCGIYSLEELKGKKS
                     EENYSVRILIKVTKSEAKVLSHNYQIEKKDNEYALTLKGSKMDNKADLLAGFYQDLVS
                     LKISPSAIEVIDNSLEELYLEVTK"
     gene            2392..3153
                     /locus_tag="VBC74_001897"
     CDS             2392..3153
                     /locus_tag="VBC74_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6942802.1"
                     /translation="MKSLAVMLKKEWMENVRTYKVISILITCSIFGILGPLTALMMPD
                     IMAGILPKKLQGAIPETTYIDSYIQYFKNMNQLGLVILVFLFSSTLTQEFSKGTLINL
                     VTKGLAKKVIILAKFIVITLLWTVSYLLSVVIHFSYTLYYFSNEGSHKLMVYGATWFI
                     GILFISLILFFSVLFRKTLGILLGCLFMVVLCVVSGFFKISKSYNPMLLIQKQGEMLS
                     GKFKVQDLMEPSILVFLLIIMTLLLSMYIFEKSEV"
BASE COUNT         1102 a          441 c          543 g         1091 t
ORIGIN      
        1 ttaagggctc ttttataatt cattattttt caatgacaaa tattagtgaa gttaattttt
       61 atttaaccat tgttattatt aatgctgttt tgccacttat taatcaacga aaaagtttct
      121 tctatggaaa cttttttata ttatatttcc tgataggaac ttttattttt gatttagaaa
      181 ctctataatg gacgtgaaag gagattgacc gatgcttgga gagaatatct atttacaacg
      241 tactcagatt ggtatgacgc aagagaattt gagtgattat ttgcatttaa caaaaactac
      301 aatttcaaag tgggaaaata atcaagctaa accggatatt gactatctta ttttgatggc
      361 taatcttttt gatattagct tggatgactt agtcggttat caaaaaacat tgtcagatga
      421 tcaaagaaat cagctcatta aggatctaaa aataaaagca aatgtattgt ctgagagaga
      481 tttctttcag gaagttaagg aattatcaaa acagttccct aacgatttta agactctgtt
      541 gataatgatt aatatggtct tgagcaacct aaccaacctt aatgattcag aaatgaaaga
      601 gtggtcacta agtacactaa ataaaattat tagtaaaaca gtagttgagt ctgacctcca
      661 gacggcgata atgttcaaaa cggtcgttct ttttcacaac cttgaatatg atgaagtgat
      721 aaacctttac cagaatagtc cctataaatt aggtgaagaa ttacttttag ctaatagctt
      781 ttttgcaaaa ggcgatgttt accgagctaa gcaagtatta caggttgaaa tttatcagca
      841 gattctactt gtatgcgagt atctattgag tttatcaact tttgaagttg aagaaaacaa
      901 aggcgatatt tttgaaaggc ttgaacatat gatagaagcg tttcaattaa actctttgca
      961 tcccaacaca gtcatcaagt gttattatag tttagcttgt cattatagta aaagtgatga
     1021 tgataaggca ttaggatatt taaaacaatg ttttaaatct ttaaaacagt tgctccaaag
     1081 atttgaatta cacggtgata ctttctttta taccatcgat gattggttag agacaatacc
     1141 tactggtatt gcaccaccga cgagtcaatt acaggtaata gaacgagttg aggacttatt
     1201 tcaaaattca caatttaaag aactatcagg gcgtaaagaa tttcaacaaa ttattcaaaa
     1261 acttaacgat ttaaaaaagg attactaata tgaatacaga aataccaaat caattattac
     1321 cattcctagt tccgttgatt atgttgcaag gagctttaat tatcatttct ctagtcaaac
     1381 taagtaaact taagatgact aaacaccttt caaagccagt ttggtttttg gtcattatat
     1441 ttcttaatat aattgggcca attgcatttc ttattttgga agggaataat gaatgaaagc
     1501 tatctcattt caaaacgtta caaaatcttt tggtcctaaa aaaatattaa ataatgtttc
     1561 ttttgattta gaagaaaata tgatttatgg attcgtagga ccaaatggag caggtaaaac
     1621 aacgacaata aaaatgattt taggattgtt aaaatttgat acgggtttca taacaatttt
     1681 tggtaaaaaa gttaattttg gaagaacaga taccaatcaa ctgattggct atctcccaga
     1741 tgttcccgag tactacgatt atatgacagc tctagaatat cttgatttat gttctggttt
     1801 agcgagatca aagcataagc tttctaacaa agaactactt aggtcagttg gacttgacga
     1861 taatcaccaa aagattgcta cttattctcg aggaatgaaa caacgtcttg gattagccca
     1921 agcactggtc catgatccta aaattattat ttgcgatgaa ccaacctctg cattagaccc
     1981 gaaaggtcgc caagatattt tagatattat ttcaaatctt agaggagaaa aaactgtgat
     2041 tttttctacc catattctct ctgatgtcga aaaaatatgt gatcacgttc tggtgttaac
     2101 taagtgtggg atttattctc ttgaagagtt aaaaggaaaa aaatcagagg aaaattattc
     2161 ggttcgtatt ttaataaaag tgacaaagtc tgaagctaag gtactatctc ataactatca
     2221 gatagaaaag aaagataatg agtatgcact gactctcaaa ggctctaaaa tggataacaa
     2281 agcagattta ctagctggtt tttaccaaga tttagtttct ttaaagatta gcccatcagc
     2341 tattgaagtc attgacaata gtttagagga gctttatttg gaggttacca aatgaaatct
     2401 ttagcagtaa tgttaaaaaa agaatggatg gagaatgtta ggacatacaa agttatatct
     2461 attctgatta cttgttcaat atttggtata ttaggtccct taactgcact tatgatgcct
     2521 gatattatgg caggaatttt acctaaaaaa ctacagggag cgataccaga gactacctat
     2581 attgattctt atattcagta ttttaaaaat atgaatcagc ttggtttagt tattttagta
     2641 tttttattta gtagtacact gacgcaagaa ttttcaaaag gaaccttaat taatctagtt
     2701 acgaagggat tagccaaaaa agttataatc cttgctaaat ttatagtaat tacccttcta
     2761 tggacagtgt catacttatt aagtgttgtc atccatttta gttatacgtt atattacttt
     2821 agtaacgaag gaagtcataa attaatggtt tacggtgcaa catggtttat cggtattctt
     2881 ttcataagct taatactctt tttttcagtt ctttttcgta aaactttagg gattttgtta
     2941 ggttgtcttt ttatggtggt gttatgtgta gtatctggat tttttaaaat cagtaaaagc
     3001 tataatccta tgcttcttat ccaaaaacaa ggagaaatgt taagtggaaa gtttaaagtt
     3061 caagacttaa tggaaccgag catattagtg tttttgctta ttataatgac gttattactt
     3121 agcatgtaca tttttgaaaa aagtgaagtt taattttgat aagatatcaa taaggcg
//
LOCUS       DATROD010000144         3135 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.146, whole genome shotgun sequence.
ACCESSION   DATROD010000144 DATROD010000000
VERSION     DATROD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.146"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..777)
                     /locus_tag="VBC74_001898"
     CDS             complement(<1..777)
                     /locus_tag="VBC74_001898"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEO6942803.1"
                     /translation="MKFENIIYGIDGSVATIMLNRPDISNGFNIPMCQEIIDAIRLMS
                     ENKDVMFLVIEAQGPIFSIGGDLKVMKDAVESDDISSLTKIAELVNQISYDLLQLEKP
                     VVMCVDGAVAGAAANIALAADFVIASKKSKFIQAFVGVGLAPDAGGLLLLSKSIGITR
                     AVQLALTGESLSAEKAEALGIVYKLCESDKIGKIKDQLLKRLSRHSINSYQAIKSLAW
                     EAAFKDWEQYKKLELQLQESLAFKQDFKEGVRAHADRRRPN"
     gene            complement(916..1566)
                     /locus_tag="VBC74_001899"
     CDS             complement(916..1566)
                     /locus_tag="VBC74_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001900118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEO6942804.1"
                     /translation="MCKYKAIIFDMDGVLFDTELFYYKRRERFLKQHGITINHLPMNF
                     FIGGNMKQVWKSVLGDQYDTWDIDKLQQDYSRYKEDNPLPYKDLIFQDCKRVIEKLHH
                     KGYLLGLASSSTRHDIMLALESFNLDTYFKVILSGEEFSESKPNPAIYNRAAELLDIP
                     KQQILIVEDSEKGIAAGIAAGIDVWAIEDKYFGMNQSQANVLVSDLSQFFVKLDNY"
     gene            1660..3012
                     /locus_tag="VBC74_001900"
     CDS             1660..3012
                     /locus_tag="VBC74_001900"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEO6942805.1"
                     /translation="MKVVKFGGSSLASSQQLYKVLNIIKSDYTRRFIVVSAPGKRYEE
                     DLKMTDALIQYYQNYINGKDIVKDQTWIINRYQEIISDLSLGSTIAEEITRSIEQLAS
                     LPIENNQFLYDCFLAAGEDNNAKLVATFFNQNDIPARYVHPNEAGIIVTKEPCNARII
                     PGSYDKIENLCLYNEVLVIPGFFGVTEDNQICTFSRGGSDITGSLIAAGIKADLYENF
                     TDVDGIFAAHPGVVKNPHAIPELTYKEMRELAYAGFSVLHDEALLPAYRGRIPLVIKN
                     TNNPQQPGTKIVLKHTRSNIAVTGIASDSRFASINVSKYLMNREVGFGRKVLQILEDL
                     NISFEHMPTGIDDLSIVLREKELTPIKEQEILNYLTRKLEVDYVDIQHNLSTIVIVGE
                     NMKSQIGVTATATQALSREKINITMISQGSSEVSIMFVIDSKDEKRAIKALYETFFQK
                     "
BASE COUNT         1075 a          584 c          468 g         1008 t
ORIGIN      
        1 attaggacgc cttctatcag cgtgtgcccg aacaccctct ttaaaatctt gcttaaaggc
       61 taatgattct tgtaactgta attcaagctt tttatattgt tcccaatctt taaaagcagc
      121 ttcccaagct aatgatttta tggcttgata tgagtttata gaatgtctac ttaaacgctt
      181 taatagttga tccttaattt tacctatttt atctgattca caaagtttat aaacaattcc
      241 caaagcttca gctttttcag cagataagct ttctccagtt aaagctagtt gcactgcacg
      301 agttataccg attgatttac taagtaacaa taaacctcct gcatctggag ctaaaccgac
      361 acccacaaaa gcctggataa atttactctt cttgcttgcg ataacaaaat cagctgctaa
      421 tgcaatatta gctgcggcac cagctacagc cccatctaca cacataacaa caggtttctc
      481 tagctgtagt agatcatacg atatctgatt aactaactca gcaattttag ttaatgagct
      541 aatatcatcg ctctcaacag catctttcat cacttttaaa tctcctccga ttgaaaagat
      601 tggtccttgt gcttcaatca ctaaaaacat gacatcttta ttttcagaca taagccttat
      661 tgcatcaata atttcttgac acattgggat gttaaaacca tttgaaatat ctggtcgatt
      721 aagcataata gtggccacgc tgccatcaat accatatatt atattttcga acttcaaaac
      781 gtctcctctc tttaagagtg aaagaaaaat aaaaaataat ttgattatca aattatcatg
      841 ctttgtattt tatcataaac ctagctttca tggcaagata aaaaataaaa aaaaacggaa
      901 ttaaacctcc cgtttttaat agttatctaa tttcacaaaa aattgactca gatcgctcac
      961 taatacatta gcctgcgact gattcattcc aaagtatttg tcttcaatag cccacacatc
     1021 aataccagct gcaatgccag ctgcaatccc cttctcactg tcttctacaa taagaatttg
     1081 ctgtttagga atatctaaaa gttctgctgc tctattataa atagctggat taggtttact
     1141 ttctgaaaat tcctcaccag acagaattac cttaaaatat gtatccagat taaaactttc
     1201 taatgctaac atgatatcat gtctagtaga acttgaagct agacccaaaa gatagccttt
     1261 gtgatgaagc ttttcaatga ctcttttaca atcctgaaaa attaaatctt tgtatggcaa
     1321 gggattatcc tctttatagc gactataatc ttgttggagc ttatcaatat cccaggtatc
     1381 atattgatca cctaaaacag atttccaaac ctgcttcata tttcctccaa taaaaaaatt
     1441 cattggtaaa tggttaattg taattccatg ttgttttaaa aaacgctctc gccttttata
     1501 ataaaataat tcagtgtcaa atagaacacc atccatatca aatattattg ctttatattt
     1561 acacatgtaa ctattatatc attcttttaa atttagaggt aatgacaaat tttctgactt
     1621 ttgttataat ttatctaaaa ttcattcttg aggtaaccta tgaaagtagt caaatttgga
     1681 ggaagctctt tagcatcttc ccaacaatta tacaaagtac taaatattat caaatcagac
     1741 tacactagac ggtttattgt cgtttcagct ccagggaaac gctacgaaga agacttaaaa
     1801 atgacagatg ctttgataca atattatcaa aattatatta acggtaaaga tattgttaaa
     1861 gaccaaactt ggattatcaa tcgttatcaa gaaattatta gtgatttgtc tttaggaagc
     1921 actattgcag aagaaattac tcgctctata gagcagctag cttcacttcc catagaaaat
     1981 aatcagtttt tatatgattg ttttttagca gccggtgaag ataacaacgc aaagttagtt
     2041 gcaacgtttt ttaatcaaaa tgacattcct gcaagatatg ttcatccaaa cgaagcagga
     2101 attattgtaa ctaaagaacc atgtaatgca cgaattattc caggaagtta tgataagatt
     2161 gagaacttat gtctatacaa tgaggttctt gttatccctg gattttttgg agtcacagaa
     2221 gataaccaaa tttgtacctt ttcaagaggg ggatctgaca ttactggatc cctaattgca
     2281 gcaggcataa aagcagacct ttatgagaac ttcacagatg ttgatggtat atttgcagcg
     2341 catccaggtg tagttaagaa ccctcacgct atccctgagc ttacttataa agaaatgcgt
     2401 gaattagcct atgcgggttt ttcggtttta catgatgaag ctttacttcc tgcctatcgt
     2461 ggcagaatcc ctcttgttat taaaaataca aataatcccc aacagcctgg tacaaaaata
     2521 gttttaaagc atactcgtag taacatagca gtaactggga tcgcttctga tagccgtttt
     2581 gctagcataa acgtatctaa atacttaatg aatagagaag taggtttcgg ccgaaaagta
     2641 ctacaaattt tagaggattt aaatattagt tttgaacata tgccaactgg catagatgat
     2701 ctatccattg tcttacgtga aaaagaattg acaccaatca aagaacaaga aatcttaaat
     2761 tacctaactc gtaaactaga agtagattac gttgacatcc aacacaattt atctacaatc
     2821 gtaattgtag gtgaaaatat gaaaagtcag attggagtca ctgcaacagc gacacaagcc
     2881 ttatcaagag aaaaaatcaa tatcaccatg atatcacaag gttcaagcga agtctccatt
     2941 atgttcgtta tagacagtaa ggatgaaaaa agagctatta aagcactata tgaaacattc
     3001 ttccaaaaat agtacctatt acactactta cactattaga tagataacaa atcgtcctaa
     3061 gtaagtttac ttaggacgat tttatttaga acataggata gttttttcaa ttttaatcgt
     3121 aactacttgg tatca
//
LOCUS       DATROD010000145         3133 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.147, whole genome shotgun sequence.
ACCESSION   DATROD010000145 DATROD010000000
VERSION     DATROD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.147"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..502)
                     /locus_tag="VBC74_001901"
     CDS             complement(<1..502)
                     /locus_tag="VBC74_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEO6942806.1"
                     /translation="MNMKLSKRYRFWQKVIKALGVLALIATLVLVVYLYKLGILNDSN
                     ELKDLVHKYEFWGPMIFIVAQIVQIVFPVIPGGVTTVAGFLIFGPTLGFIYNYIGIII
                     GSVILFWLVKFYGRKFVLLFMDQKTFDKYESKLETSGYEKFFIFCMASPISPADIMVM
                     ITGLSNM"
     gene            595..944
                     /gene="ssrA"
                     /locus_tag="VBC74_001902"
     tmRNA           595..944
                     /gene="ssrA"
                     /locus_tag="VBC74_001902"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1213..1851
                     /locus_tag="VBC74_001903"
     CDS             1213..1851
                     /locus_tag="VBC74_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HEO6942807.1"
                     /translation="MASILKKHLEKRLELKNDPMRTDMSTGASYPKYGFSVGKYTYGY
                     QQFFYEGVNLKEIGAFCSIAQNVTITGMNHPTDHITTNPFIYYKSRGFINEDRADLID
                     EKKNGKVIIGNDVWIGTNVTILPSVTIGNGAIIEAGSVITKDIPDYAVVAGTPAKIIK
                     YRFSEEEITLLNASQWWNWSDEAIKEHISEFSDKREFFNTLKSISENKNHKL"
     gene            complement(1969..3072)
                     /locus_tag="VBC74_001904"
     CDS             complement(1969..3072)
                     /locus_tag="VBC74_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6942808.1"
                     /translation="MSNIITYLKNNSNLTFDELALNDVDILCLNEFGYISFEKLINTT
                     EMKSVLVCELYHEYLQTMAKSYSFMFTSQRHDLCQLMMTSKRFKNLTLSYYQAEISLE
                     FEKQFAAMVFTIPNIDYHQVVFRGTDANLIGWKEDFKLTYMREISAHRSAIKYLNTIL
                     PYFDKVVLSGHSKGGNLALYAAMFTKPDLKAKIDLIWLIDSPGLQKTLLPTTEYKTTK
                     QKCIRLLPEESIVGMMLYSDIEPLIISSNARGILQHDVTTWEIQEPAILKTGAGLSLK
                     SICFEKTFQQWMAELKSQERKLFFDLLFDSFLSSGVSSLDDFNLASRAKMMKAFHSFR
                     ELDDDKKRLFNKSLKLLVTIFWGAYHDNSRETK"
BASE COUNT         1079 a          534 c          487 g         1033 t
ORIGIN      
        1 acatattaga tagaccggta atcatcacca tgatgtccgc tggtgaaatg ggagatgcca
       61 tacaaaagat aaaaaacttt tcatatccgg atgtttctag cttagactca tatttatcaa
      121 atgtcttctg atccataaat agcaaaacga attttcgtcc ataaaatttg actaaccaaa
      181 aaaggatgac acttccgatg ataatcccaa tatagttata aataaagcct aaggtaggac
      241 caaaaattaa gaaacctgct actgttgtaa cacctccagg aattactgga aaaacaatct
      301 gtacaatttg agccacaata aaaatcattg gaccccaaaa ttcatacttg tgtactaggt
      361 cttttaattc attgctatca ttcaaaatac caagtttgta caaataaaca actaagacaa
      421 gggtcgctat taacgctaag acacccaaag ccttaatgac cttctgccaa aagcggtaac
      481 gttttgataa tttcatattc atgctttaat cataacacaa ctgttaaatt tttgctattt
      541 taaatagtat ttgttatact atattggtaa gtttttatgc tttattctac ttttggggtc
      601 gttacggatt cgacaggcat tatgaggtat attttgcgac tcatcggcag atgtaaaatg
      661 ccagttaaat ataactgcaa aaaatacaaa ttcttacgca ttagctgcct aaaaaacagc
      721 ctgcgtgatc ttcacaagat tgtttgcgtt ttgctagaag gtcttattta tcagcaaact
      781 acgtttggct actgtctagt tagttaaaaa gagatttata gactcgctat gtgagggctt
      841 gagttatgtg tcatcaccta gttaaatcaa tacataacct atagttgtag acaaatatat
      901 tagcagatgt ttggacgtgg gttcgactcc caccggctcc atatcatttt atcataactt
      961 actatacata cttaaaacct tgttattaca aggtttttat tttgtttctt tgctcataca
     1021 ttgcccctat cttaggaaaa aaatacttgg aacagactct aacattgaat taggccacct
     1081 cattagctca ctctttgtca atatccgaga ataagttctt tatcattcta atttatagaa
     1141 tttattaaaa aagataaggt ttttagagga aaaaatgtta taatctattt agcctacgat
     1201 tggaggctag ttatggctag tattttaaaa aaacatttag agaaacgttt agagcttaaa
     1261 aatgatccta tgaggacaga tatgagtact ggtgccagtt accctaaata tggattctcg
     1321 gtaggaaagt acacatatgg atatcaacaa ttcttttacg aaggcgttaa cttaaaagaa
     1381 attggagcat tttgctcaat agcccagaac gttactatta ctggtatgaa tcatccaacc
     1441 gatcacatta ccactaaccc ttttatttac tataagtcaa ggggatttat aaatgaggat
     1501 agggcggact taatagatga aaagaaaaat ggaaaagtta ttattggcaa cgatgtctgg
     1561 ataggaacta atgtcactat acttccatct gttacaatag gtaatggagc aattattgaa
     1621 gcaggttcag ttattaccaa agacattcct gactatgctg ttgtcgctgg aacacctgct
     1681 aaaattatca aatatcgctt ttcagaggaa gaaataacat tattaaatgc atctcagtgg
     1741 tggaattggt cagatgaagc tattaaggaa catattagcg aattttcaga taagagggaa
     1801 ttcttcaata ctctaaaaag tatttctgaa aacaaaaatc ataaactcta gaatgacttc
     1861 atcaaatagc acactaaaat actactcagc ataatatgag tagtatttta gtatgctatt
     1921 ttgctagttt atttagcaca tgtttagact aatgcaattc taggaaagct acttagtctc
     1981 tctgctatta tcatgataag ctccccaaaa tatggttact aataatttta aagatttgtt
     2041 aaataacctt tttttatcat catctagttc tctaaagcta tgaaaagctt tcatcatttt
     2101 tgctctggaa gccaagttga agtcatccaa actagatacg ccacttgata ggaagctatc
     2161 aaataataaa tcaaaaaata attttctttc ttgacttttt aattcagcca tccactgttg
     2221 aaatgtcttt tcaaaacaaa tactttttag cgatagtcct gctccagtct ttaaaatagc
     2281 tggttcttga atttcccaag ttgtaacatc gtgttgcaga attccacgcg cattgctact
     2341 aataattagc ggctctatat cactataaag catcatacca acaatagatt cttctggtag
     2401 taagcgaatg cacttttgtt tagtagtttt atactctgta gtaggtaaca aagttttttg
     2461 tagtcctggg gagtcaataa gccaaatcaa atcaattttt gcttttaaat ctggctttgt
     2521 aaacattgca gcgtacaagg ctaaattacc acccttagaa tgacctgaga gaactacttt
     2581 atcaaaataa ggcagtattg tatttaagta tttaatcgct gaacgatgag ccgatatttc
     2641 tctcatatac gtcaatttaa aatcttcttt ccaaccaata aggttggcat cagtaccacg
     2701 aaatacaacc tgatgataat caatattagg aatagtaaaa accatagctg caaattgttt
     2761 ttcaaattct aaactaatct cagcttgata ataagataaa gttaagtttt taaaacgttt
     2821 cgaggtcatc attaactgac ataaatcatg tcgttgtgac gtgaacataa agctatatga
     2881 tttagccata gtttgaaggt actcatgata taactcacag actaaaactg acttcatttc
     2941 tgtagtgttt ataagttttt caaaagaaat atagccaaat tcatttaaac aaagaatgtc
     3001 aacatcgttt aatgctaatt catcaaaagt taagtttgaa ttatttttta aataagttat
     3061 tatatttgac atcaccgttt attctttcta ctgctgaata atttccgaac ttcatctaaa
     3121 cctatcacga taa
//
LOCUS       DATROD010000146         3118 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.148, whole genome shotgun sequence.
ACCESSION   DATROD010000146 DATROD010000000
VERSION     DATROD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.148"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            54..626
                     /locus_tag="VBC74_001905"
     CDS             54..626
                     /locus_tag="VBC74_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEO6942809.1"
                     /translation="MRKRSILPILAVLFVFMSIFSVYSIFHGDKAKEKASVIKQASQT
                     SQTSKKEVLQKKTYPNLNKYSNLEIHVSSTRQTMTITSSDKVIFKTIVSTGAKESPTP
                     KGTFVIEPERGDFFYNASSKEGAYYWVSFKEHGIYLFHSVPTDQQGNEIPEEAKQLGK
                     AASHGCVRMSRADAKWFYENIPQGTTVTIK"
     gene            902..1753
                     /gene="ylqF"
                     /locus_tag="VBC74_001906"
     CDS             902..1753
                     /gene="ylqF"
                     /locus_tag="VBC74_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEO6942810.1"
                     /translation="MATIQWFPGHMSKARRQVQENIKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADPIRTKEWRDFYESQGLKTLAINSKEQSTVKKVTDIAK
                     ILMSDKIAKLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFKTYY
                     PDRLKERFKSINLEDESPEIIMTLTQKLGYRDDYDRFYNLFVKEVRDGKLGRYTLDIV
                     GEHDGNN"
     gene            1740..2501
                     /locus_tag="VBC74_001907"
     CDS             1740..2501
                     /locus_tag="VBC74_001907"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEO6942811.1"
                     /translation="MATIKEIKAILETIVDLKDKRWQEYQTDSRAGVQKAILQRKKNI
                     QSHLDEEARLEQMLVYEKKLYIEHINLIAGIDEVGRGPLAGPVVAAAVILPPNCKIKH
                     LNDSKKIPKKKHQEIYQNILDQALAVGIGIQDSQCIDDINIYEATKHAMIDAVSHLSV
                     VPEHLLIDAMVLDLPIPQTKIIKGDANSLSIAAASIVAKVTRDKIMSDYDSKYPGFAF
                     SKNAGYGTKEHLEGLQKYGITPIHRKSFEPIKSML"
     gene            2522..3070
                     /locus_tag="VBC74_001908"
     CDS             2522..3070
                     /locus_tag="VBC74_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEO6942812.1"
                     /translation="MTEKEKMLAGQYYRPSAPELRKDREVALKNMQAFNNEDDSSKRN
                     VILQKWFGATGKSIHMEQRFVCDYGCNIYVGENFYANFNQTFLDVCEIRIGDNCMFGP
                     NCQLLTPLHPLDPIERNSGLEYGAPIQIGNNVWLGGGVTILPGVVLGDNVVVGAGSVV
                     TKSFENNVVIAGNPAKIIKKLA"
BASE COUNT         1144 a          513 c          570 g          891 t
ORIGIN      
        1 tgaaacttat aatagatttg tccataacag agcaaaagag agagggatag tacatgagaa
       61 aacgttcaat tttgccaata ttagctgtct tatttgtttt catgagtatc tttagcgtat
      121 actctatttt tcatggtgat aaagctaaag aaaaagcaag tgtcataaaa caagcatctc
      181 aaacaagtca aacttcaaaa aaagaggtct tacaaaagaa gacttatcca aatctaaata
      241 aatattcaaa tctagaaatt catgtttcat ctacgaggca aacaatgaca ataacctcga
      301 gtgacaaggt catctttaaa acgattgttt caacaggagc aaaagagagc cctaccccaa
      361 aaggaacttt tgtcatagaa ccggaacgag gagatttttt ctataacgcc tcatcaaaag
      421 aaggagccta ttattgggtc tcctttaaag agcatggtat ttatctcttt catagcgttc
      481 cgactgatca acaaggaaat gagatcccag aagaagcaaa gcaattagga aaagccgcct
      541 cacatggctg tgtgcgtatg tcaagggcgg atgcaaaatg gttctatgaa aatattcctc
      601 aaggaacgac tgttaccatc aaatagtctt aatgtggtaa aaatcatgtt attaaaagct
      661 tggagtaaac ttccagagct ttttcgtata tcccttaaac tcaattagta aacgaaacct
      721 atcagcggaa taaatgtata aagtatttcg atatcattaa aaaaatgata gaataatagt
      781 gttaatcgaa tattttatcg acctttatat gcggttgaag aagaataaaa ctaaaagatt
      841 agacctctaa caaatagatg tagactttta aggtagtcta tacataagga gaatttattt
      901 catggctact attcaatggt tcccaggaca catgtccaaa gctcgccgtc aagttcaaga
      961 aaatattaag catgttgatt ttgttacaat tttagtggat gcaaggttac ctctttctag
     1021 tcaaaatcca atgctaacca aaatcgtcgg agacaaaccc aaactaatga tcctcaataa
     1081 agctgatcta gcagatccca ttcgaactaa agaatggaga gatttttatg aatctcaagg
     1141 tttaaagaca ttagctatta attctaaaga acaatcaaca gttaaaaaag taaccgacat
     1201 cgctaaaata ttaatgtccg ataaaatcgc taaactacgg gaacgtggta ttcaaaaaga
     1261 gacacttcgt acgatgatta ttggaattcc gaatgcaggc aaatcgactc taatgaatcg
     1321 cctagcaggt aaaaaaattg cagtggttgg taataaacca ggtgtcacaa aaggtcagca
     1381 atggctcaaa tctaataagg aactggaaat tttggacact cctggtatct tgtggcccaa
     1441 atttgaagat gaattagttg gattaaagct agcattaaca ggggcaatca aagatcaatt
     1501 attgcctatg gatgaggtaa caatatttgg attgaattac tttaaaacct attatcctga
     1561 tcgtctcaaa gagcgtttta aaagtattaa tttagaggat gaatctcctg aaatcatcat
     1621 gaccttaaca caaaaattag gttatcgtga cgattacgat cgcttttata atttatttgt
     1681 caaagaagtt cgcgatggta aattaggacg ctatacgtta gatatcgtcg gagaacatga
     1741 tggcaacaat taaagaaatc aaagcaattt tagagactat tgttgattta aaagacaaac
     1801 gttggcaaga atatcaaaca gattcacgcg caggtgttca aaaagcaatc cttcaacgaa
     1861 aaaaaaatat tcagtcacat ttagacgaag aagcgcgctt agaacaaatg ttggtctacg
     1921 aaaaaaaact ttacattgaa catataaacc ttattgcagg aatcgatgaa gtaggtagag
     1981 gtcctttagc aggaccagta gtagctgctg ctgttattct tccaccaaac tgtaaaataa
     2041 aacatctcaa tgactccaaa aaaattccaa agaaaaagca tcaagaaatc tatcaaaata
     2101 ttttagatca agcgttagca gttggtattg gtattcaaga cagccaatgt attgatgata
     2161 tcaacattta tgaagctaca aaacatgcta tgattgatgc agtttctcat ttatctgtag
     2221 ttcctgaaca tcttttaatt gatgctatgg ttttggatct accaattcca caaactaaaa
     2281 ttatcaaagg tgatgctaat tcactctcta ttgcagcagc ttcaattgta gctaaagtga
     2341 ccagagataa aataatgtct gattatgata gcaaatatcc tggatttgca ttttctaaaa
     2401 atgctggcta tggtactaaa gagcatttag agggtttgca aaaatacggt attactccga
     2461 tacatcgtaa aagttttgaa cctataaagt caatgttgta aataatacgg aagaggaaaa
     2521 gatgaccgaa aaagaaaaaa tgttagcagg acaatattat cgtccatcag cacctgaact
     2581 acgcaaggat agagaagttg cactaaaaaa tatgcaagcc tttaacaatg aagatgatag
     2641 tagcaaacgt aacgtcattt tacaaaaatg gtttggtgct actgggaaga gtatccatat
     2701 ggaacaacgt tttgtctgcg attatggttg taatatttat gttggtgaaa acttctacgc
     2761 caattttaac caaactttct tagatgtttg tgaaattcgt attggcgata actgtatgtt
     2821 tggcccaaac tgtcaactcc tcaccccact ccatcctcta gatccaatag aaagaaattc
     2881 aggattagaa tacggtgccc caatccaaat cgggaataat gtctggctag gtggaggagt
     2941 gactatttta ccaggtgttg tattaggaga taatgtcgtc gttggcgctg gaagtgtcgt
     3001 tacgaaatca tttgaaaata atgttgttat agcaggaaac cctgctaaga taatcaaaaa
     3061 attagcttaa tgtaagaaaa aacctttaat tataacaaag gttttttcta atttaaaa
//
LOCUS       DATROD010000147         3042 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.149, whole genome shotgun sequence.
ACCESSION   DATROD010000147 DATROD010000000
VERSION     DATROD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.149"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..240
                     /gene="tilS"
                     /locus_tag="VBC74_001909"
     CDS             <1..240
                     /gene="tilS"
                     /locus_tag="VBC74_001909"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEO6942813.1"
                     /translation="IILRRRKEGDRIFLGNHTKKIRRLFIDEKITLKDREEAVIGEQN
                     KELIFVIVAGRTYLRKPSEHDIMKGKLYIENLEKR"
     gene            245..787
                     /gene="hpt"
                     /locus_tag="VBC74_001910"
     CDS             245..787
                     /gene="hpt"
                     /locus_tag="VBC74_001910"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEO6942814.1"
                     /translation="MLENDIKKVLYSEEDIILKTKELGAKLTADYAGKNPLLVGVLKG
                     SVPFMAELLKHIDTHVEIDFMVVSSYHGGTTSSGEVKILKDVDTNIEGRDVIFIEDII
                     DTGRTLKYLRDMFKYRQANSVKVATLFDKPEGRLVDIDADYVCYDIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEIYSK"
     gene            810..2786
                     /gene="ftsH"
                     /locus_tag="VBC74_001911"
     CDS             810..2786
                     /gene="ftsH"
                     /locus_tag="VBC74_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEO6942815.1"
                     /translation="MKNNKNNGFLKNSFIYILLIIAVITTFQYYLKGTSSQNQQISYT
                     KLVKQLKAGEIKSISYQPSGGVVEVSGTYKKAKTIKSANSFTFLGGSVATKVTGFNSV
                     ILPNDSSIKSLVSAAEENNTNIQVKHESSSGTWISYIASFLPLVIMIGFFMMMMNQGG
                     GGGARGAMSFGKNKARSSSKDEVKVRFSDVAGAEEEKQELIEVVDFLKDPKRYKSLGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDA
                     KKAERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNESIIVIAATN
                     RSDVLDPALLRPGRFDRKVLVGQPDVKGREAILRVHAKNKPLADNVDLKVVAQQTPGF
                     VGADLENVLNEAALVAARRNKKVIDASDIDEAEDRVIAGPSKKDRTISERERAMVAYH
                     EAGHTIVGLILSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKDDMKEQLAGLMGG
                     RVAEEIIFNAQTTGASNDFEQATAMARAMVTEYGMSEKLGPVQYEGNHAMMAGQMSPE
                     KSYSAQTAQLIDDEVRHLLNEARNKAADIINENRDTHKLIAEALLKYETLDAAQIKSI
                     FETGKMPETENDEDKARALSYDEIKEKMQEEDKK"
BASE COUNT         1071 a          429 c          631 g          911 t
ORIGIN      
        1 attattttaa gaagaagaaa agaaggggat aggatatttt taggaaatca cactaaaaaa
       61 attcgccgtc tatttattga tgagaaaata actctaaaag acagagaaga agctgttatt
      121 ggggagcaaa ataaagagtt gatatttgtg attgttgcag gtagaacata tttgagaaaa
      181 ccttctgaac atgatataat gaagggcaaa ctttatattg aaaatttaga aaaaaggtga
      241 catcatgctt gaaaatgata taaaaaaagt tttatattca gaagaagata ttattctgaa
      301 aacgaaagaa ttaggagcta aattaactgc agattatgca ggtaagaatc cattattagt
      361 aggtgtatta aaggggtcag tgccttttat ggctgaacta ttaaagcata ttgatacaca
      421 tgttgaaatt gattttatgg ttgtttcaag ctatcatggt ggtacaacta gtagtggaga
      481 agttaaaatt ttaaaagatg ttgatactaa cattgaagga agagatgtaa tctttattga
      541 agacatcatt gatactggac gtacattaaa ataccttcga gatatgttta agtatcgtca
      601 agctaactct gtcaaagttg caaccctttt tgataagcca gaagggcgat tggtagatat
      661 tgatgctgat tacgtttgtt acgatatccc aaatgaattt attgtgggat tcggtttaga
      721 ctatgctgaa aactatcgta acctacctta tgtcggtgtg ttaaaagaag aaatttactc
      781 aaaatagaaa aaaggtactc tcttaaacta tgaaaaataa taaaaataat ggttttctga
      841 aaaattcctt tatttacata ttattgatta ttgcggttat tacaaccttt caatactatt
      901 taaaaggaac tagctctcaa aaccagcaaa tttcttatac aaaacttgtt aaacaattaa
      961 aagctggaga aattaagtct atttcatacc agccaagtgg tggtgttgtt gaagttagtg
     1021 ggacatataa gaaagccaaa acaattaaat cagctaatag ttttactttt ttaggtggta
     1081 gtgttgctac taaagttaca ggatttaatt ctgttatact accaaatgac tcttcaataa
     1141 aatctcttgt tagcgctgct gaagaaaata acactaatat tcaggtcaaa catgaaagtt
     1201 caagtgggac atggatttct tatattgcca gttttttacc tttagttatc atgattggtt
     1261 tctttatgat gatgatgaac caaggcggtg gcggtggcgc tcgtggtgcc atgagctttg
     1321 gtaaaaataa agcaagatca tctagtaaag atgaggttaa agttcgattt tcggatgttg
     1381 ctggagcaga ggaagaaaag caagaattaa tagaagtcgt tgacttcctt aaagatccta
     1441 aacgatataa atctctaggt gcccgaattc cagcaggggt tctattagaa ggacctccag
     1501 ggacaggtaa aaccctatta gcaaaagctg ttgccggtga agcaggggtt ccattcttta
     1561 gtatttccgg ttcagatttt gttgaaatgt ttgtcggtgt tggagctagt cgagtacgct
     1621 ctttgtttga agatgctaaa aaagctgaac gtgctattat tttcattgat gaaattgatg
     1681 ctgttggacg tcgacgtgga gctggtatgg gtggcggaaa cgatgaacgt gaacaaacgc
     1741 tcaaccaact tttaattgaa atggatggtt ttgaaggaaa cgaaagtatt atcgttatcg
     1801 ctgctaccaa ccgtagtgac gtgttagatc ccgcattact gcgtccagga cgttttgata
     1861 gaaaagttct agttggacaa cctgatgtaa aaggtcgtga agcaattctg agagtacatg
     1921 ctaaaaataa accattagca gataatgttg acttaaaagt agtagctcaa caaaccccag
     1981 gatttgttgg tgctgatttg gagaatgttc tcaatgaagc tgctttagtt gctgcgcgac
     2041 gaaataaaaa agttattgat gcttcagata ttgatgaagc agaagaccgt gtaattgctg
     2101 gtccttctaa aaaagatcgt actatttctg aaagagaaag agctatggtt gcttaccatg
     2161 aagctgggca cacaattgtc ggtttgattc tttcaaatgc gcgagttgtt cataaagtta
     2221 ctatcgtacc acgtggtcgt gcaggtggat atatgattgc tttacctaaa gaagatcaaa
     2281 tgcttctttc aaaagatgat atgaaagagc aattagcagg tcttatggga ggacgtgtag
     2341 cggaagaaat tattttcaac gctcagacaa caggtgcctc aaatgacttt gaacaagcca
     2401 ctgccatggc ccgcgctatg gtaactgaat atggtatgag tgagaaactt ggcccagttc
     2461 aatacgaagg taaccatgca atgatggctg gccaaatgtc acctgaaaaa tcatattctg
     2521 ctcaaactgc acaacttatt gatgatgaag ttcgtcacct tcttaatgaa gcacgtaata
     2581 aggcagctga tattatcaac gaaaaccgtg atactcataa attaattgca gaagccctct
     2641 taaaatatga gactcttgat gctgctcaga ttaaatcgat tttcgagaca ggtaaaatgc
     2701 ctgagacaga aaatgatgaa gataaagcga gagcgttatc atacgatgaa atcaaagaaa
     2761 aaatgcaaga agaagataaa aaataagtca tcatatattt ttaaagaact ttataaattt
     2821 caaacaccct tcaaatttag ataaaatttg aggggtgttt tttatgaaga tgaagtttaa
     2881 atttgtgaat taagaagatg aaatatgaac atgacaacgg taaaaatgct atccattaaa
     2941 caagtataga aggttagtta ctataaataa tattaacttt ttaaaaataa aagaaaaaat
     3001 gcaaaaatag tattgacagg aagtagaaat atttagtata at
//
LOCUS       DATROD010000148         3007 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.150, whole genome shotgun sequence.
ACCESSION   DATROD010000148 DATROD010000000
VERSION     DATROD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.150"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            7..471
                     /locus_tag="VBC74_001912"
     CDS             7..471
                     /locus_tag="VBC74_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEO6942816.1"
                     /translation="MAIKNTSDNIEEYIKSLLEQSGIAEIKRSNLADTFQVVPSQINY
                     VIKTRFTESRGYVVESKRGGGGYIRIAKVHFSDQHQLFGNMLSTIGERISEQVFDDLI
                     QLLFDEEIITEREGNLILATSGDDVLGEQASVIRARMLRKLLQRLDRKGFHL"
     gene            468..2915
                     /locus_tag="VBC74_001913"
     CDS             468..2915
                     /locus_tag="VBC74_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEO6942817.1"
                     /translation="MSHYSIKLQEVFRLAQFQAARYESHYLESWHLLLAMVLVHDSVA
                     GLTFAEYESEVAIEEYEAATILALGRAPKEEITNYQFLEQSPALKKILKLAENISIVV
                     GAEDVGTEHVLLAMLVNKDLLATRILELVGFRGQDDGESVRMVDLRKALERHAGFTKD
                     DIKAIYELRNPKKAKSGASFSDMMKPPSTAGDLADFTRDLSQMAVDGEIEPVIGRDKE
                     ISRMVQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGNIPYELRDMRVLELDMMS
                     VVAGTRFRGDFEERMNQIIADIEEDGHIILFIDELHTIMGSGSGIDSTLDAANILKPA
                     LARGTLRTIGATTQEEYQKHIEKDAALSRRFAKVLVEEPNLEDAYEILLGLKPAYEAF
                     HNVTISDEAVMTAVKVAHRYLTSKNLPDSAIDLLDEASATVQMMIKKNAPSLLTEVDQ
                     AILDDDMKSASKALKASHKDKKHKPIAVTEDHIMATLSRLSGIPVEKLTQADSKKYLN
                     LEKELHKRVIGQDDAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKALA
                     EVLFDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTEKVRNKPYSVLLFD
                     EVEKAHPDIFNVLLQVLDDGVLTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFGA
                     KDISHDYTAMQKRIMEELKKAYRPEFINRIDEKVVFHSLSQDNMREVVKIMVKPLILA
                     LKDKGMDLKFQPSALKHLAEDGYDIEMGARPLRRTIQTQVEDHLSELLLANQVKEGQV
                     IKIGVSKGKLKFDIAKS"
BASE COUNT          975 a          462 c          640 g          930 t
ORIGIN      
        1 gaatttgtgg ccataaaaaa tacttccgat aatattgaag aatatataaa atcattatta
       61 gaacaatcag gaattgctga aataaagcgt tccaatttag ctgatacttt tcaagttgtt
      121 cctagtcaga ttaattatgt cattaagaca cggtttactg aaagccgtgg ttatgtggtt
      181 gagagtaaac gaggtggtgg aggttacatc cgcattgcta aggttcattt ttctgaccaa
      241 caccaattat ttggaaatat gttatcaacg attggcgagc gtatcagtga acaagtcttt
      301 gatgatttga ttcaattatt attcgatgaa gagataatta cagaacgtga aggaaatttg
      361 attctggcga catcaggtga tgacgtctta ggagagcaag cctctgttat tagagctcgt
      421 atgctccgta aattattaca acgattagat agaaaaggat tccatttatg agtcattatt
      481 ccataaaatt acaagaagta ttcagattgg cgcaatttca agcagcacgc tatgaaagtc
      541 actatttaga aagctggcat cttcttttag caatggtttt agttcatgat tcagtagctg
      601 gtttgacatt tgctgaatat gagtctgaag ttgcaattga agagtacgaa gcagcaacaa
      661 ttttagcttt aggtagagca ccgaaagaag agattaccaa ttatcaattt ttagagcaat
      721 caccagcttt gaaaaagatc ttaaagttag cagaaaatat tagtatcgta gtaggagctg
      781 aagatgtggg tactgaacat gtcttgcttg ctatgttggt taataaagat ttattagcca
      841 cacgtatctt agaattagtt ggctttagag gtcaagacga tggtgaatca gttcgtatgg
      901 ttgatttacg taaggctcta gaacgtcatg ccggttttac aaaagatgat atcaaagcaa
      961 tttatgaatt acgtaatcct aaaaaagcaa agtcgggtgc ttctttttct gatatgatga
     1021 aaccaccaag tactgctggt gatttagctg atttcacacg tgatttatca cagatggcgg
     1081 ttgatgggga aattgaacct gttattgggc gtgataaaga aatttcacgt atggtccaag
     1141 tcttaagtcg taaaacaaag aataatcctg tcttagttgg agatgcgggt gtcggtaaaa
     1201 cagctttggc gtacggtcta gcacaacgta ttgctaatgg aaatattcct tatgagctta
     1261 gagatatgcg tgttctagag ttagatatga tgagcgttgt tgctggaact cgttttcgtg
     1321 gtgattttga ggaacgtatg aatcaaatca ttgcagatat tgaagaagac ggtcatatta
     1381 ttcttttcat tgatgaactg catactatta tgggttctgg ctctggtatt gatagcacac
     1441 ttgatgctgc taatatttta aaaccagctt tggctcgagg cactttacgc actattggag
     1501 caacaactca agaggaatat caaaagcata ttgaaaaaga tgctgcttta tctcgtcgct
     1561 ttgcaaaagt tttagtagaa gaaccaaatc ttgaagacgc atatgaaatt ctacttggat
     1621 tgaagccagc gtatgaagcc tttcataatg ttaccatttc agatgaagca gttatgactg
     1681 ctgtaaaagt ggctcatcgt tatttgacaa gtaagaattt accagactca gctatagact
     1741 tacttgatga agcttctgcc actgtgcaaa tgatgattaa gaaaaatgct ccatcgcttt
     1801 taacagaagt tgaccaagct attttagatg atgatatgaa atctgcttct aaagctctaa
     1861 aagcaagtca taaggataaa aagcataagc ctattgccgt aaccgaagat catattatgg
     1921 caactctcag ccgtctatct ggtattcctg ttgaaaaatt gacacaagct gatagtaaaa
     1981 aatacctcaa tttagaaaaa gaattgcata aacgtgttat cggtcaagat gatgcagtta
     2041 ctgccattag tcgtgctatc cgtcgcaatc aatctggcat tcgtactggt aagcgtccta
     2101 ttggttcctt tatgttcttg ggaccaactg gtgttggtaa gacagagtta gcaaaagcct
     2161 tggcagaagt gttgtttgat gatgaatcag ccctaattcg ttttgatatg tcggaatata
     2221 tggaaaaatt cgcagcttct cgcctgaatg gggcacctcc aggttatgtt ggttatgatg
     2281 aaggaggaga gctgactgaa aaagtacgca ataaaccata ttcagtttta ctttttgatg
     2341 aagttgaaaa agctcatcca gatattttta atgtattatt acaagtgctt gatgatggcg
     2401 ttttaacaga tagtcgagga cgaaaagttg acttttcaaa tactataatc attatgactt
     2461 ctaatttagg agcaactgca ttacgtgacg ataaaacagt tggttttgga gctaaggata
     2521 tcagccatga ttatactgct atgcaaaaac gtattatgga ggagcttaaa aaagcttatc
     2581 gaccagagtt tatcaatcgt attgatgaga aagttgtatt ccatagtctc agtcaagata
     2641 atatgcgtga agttgtgaaa attatggtta aacctttaat tttagcgctt aaagataaag
     2701 gtatggatct gaaattccag ccttcagcct tgaaacactt ggcagaggat ggctatgata
     2761 tagaaatggg tgctcgtcca ctgcgtagaa ctattcaaac gcaagtagag gatcatttat
     2821 cagaactact attggcaaat caagtgaaag aggggcaagt aattaagatt ggcgtatcca
     2881 aaggcaaatt gaaatttgat attgcaaaat cataaaacat tccagtaccg atgggtactg
     2941 gaattttaat ataaaaagaa aatgtccaaa atattttgga cattttctta tagatttatg
     3001 aaaagta
//
LOCUS       DATROD010000149         2942 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.151, whole genome shotgun sequence.
ACCESSION   DATROD010000149 DATROD010000000
VERSION     DATROD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.151"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..539
                     /locus_tag="VBC74_001914"
     CDS             <1..539
                     /locus_tag="VBC74_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942818.1"
                     /translation="LGSSRDYFLYFKDSNFRDKVFDVITKSFNNKNQNIKIDIKNQNI
                     KGDNYESGSSRNDVSGSGNIVGTNIDNGSQVSLSGDISNGSTIIKDSNINNADIHKSV
                     EIPWTDMVEQLNLIINTQLSTADKAIYSELLEISEAEDERKLKNFVNKMSSFFKNDYV
                     QNTITGVLGNVISSILMK"
     gene            557..1855
                     /gene="purB"
                     /locus_tag="VBC74_001915"
     CDS             557..1855
                     /gene="purB"
                     /locus_tag="VBC74_001915"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEO6942819.1"
                     /translation="MISRYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VAKIREKADFDIDRILEIEQDTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEKYVCDKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDTKVTSCLTQEEIDELFNPIYYTKRVDDIFERLGLEK"
     gene            2008..2919
                     /locus_tag="VBC74_001916"
     CDS             2008..2919
                     /locus_tag="VBC74_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6942820.1"
                     /translation="MLKHFGSKVRNLRVTRNITREDFCGDETELSVRQLARIESGQSI
                     PNLTKAHYIAKQLNVKLDILTGGESLELPKRYKELKYLILRIPTYADAERLKLRECQF
                     DHIFEEFYDNLPEDECLAIDSLQAKFEVYQTGDINFGVEVLCECFDKVKYKEKYTLND
                     LIIIDLFLTCAVVSKFNNRAFTKEVFQTICKTLISQNHKLTAEDLFWFNHVLLNCVFV
                     GLCLNSEECLAEMLEVSRQTMVSTHDFHKMPLYFMYQWKYFITIDNDIKSAENAYQQS
                     IMFSKMIDDKHLIKKLELEWQEDITGH"
BASE COUNT         1001 a          447 c          613 g          881 t
ORIGIN      
        1 acttgggctc tagcagggac tattttcttt attttaaaga ttcgaatttt agagataaag
       61 ttttcgatgt aattacaaaa tcgttcaata acaaaaatca aaatataaaa attgatataa
      121 aaaaccaaaa tataaagggc gataattatg aatctggatc tagtcgaaat gatgtctcag
      181 gtagtggtaa tatagttgga acaaatattg ataacggtag tcaagttagt ttatctgggg
      241 atatatcaaa tggttctact atcataaaag attctaatat taataatgcc gatatacata
      301 aaagtgtgga aattccatgg actgatatgg ttgagcagtt aaatttaatt attaacactc
      361 agctatctac tgcggataaa gcgatatatt cagaattgtt ggaaattagt gaggcagagg
      421 atgaaagaaa attgaaaaat tttgtcaata aaatgtctag tttcttcaaa aatgattatg
      481 ttcagaatac cataactggg gtgttaggca atgtcatttc tagtatttta atgaaataag
      541 ttaaaggaga caaacaatga tttcacgtta ttcacgccct gagatggcgg caatttggac
      601 agaggaaaat aaataccgtg cttggttgga ggtcgagatt ttggctgacg aggcatgggc
      661 tgagttgggt gagattccta aagaggatgt ggctaagatt cgcgagaagg cggattttga
      721 cattgaccgc attcttgaga ttgagcagga cacgcgtcac gatgtggtgg ctttcactcg
      781 tgcggtttct gagacgcttg gtgaggagcg caagtgggtg cactacggtt tgacgtcgac
      841 tgacgtggtg gacactgcct acggttacct ctacaagcag gctaacgaca ttatccgtcg
      901 tgatcttgag aatttcacaa acatcgtagc tgacaaggct aaggagcaca agttcaccat
      961 catgatgggt cgtacccacg gtgttcacgc cgagccaacg actttcggtc tcaagttggc
     1021 gacttggtac agcgagatga aacgtaatat tgagcgtttt gaacatgctg ccgcaggtgt
     1081 ggaagctggt aagatttcag gtgccgttgg taactttgcc aacattcctc catttgtgga
     1141 aaaatatgtc tgtgacaaat tgggcatccg tccgcaagaa atttcaacac aggttcttcc
     1201 acgtgacctc cacgcagaat attttgcagt acttgcaagc atcgcaacat ctatcgaacg
     1261 tatggcgaca gagattcgtg gtctgcaaaa atcagaacaa cgtgaagttg aagaattctt
     1321 tgccaaaggt cagaaaggta gctctgctat gcctcacaaa cgcaacccaa tcggttcaga
     1381 gaacatgaca gggctagcgc gcgtgattcg tggtcacatg gtgacagctt atgagaacgt
     1441 gtcactttgg catgagcgtg acatttcgca ctcatcagct gagcgtatca tcacaccaga
     1501 cacaactatc ttgattgact acatgctcaa ccgttttggc aatatcgtta agaacttgac
     1561 tgtcttcccg gaaaatatga tgcgcaatat ggaatcaact tttggtttga tttatagtca
     1621 acgtgttatg ctcaaattga ttgaaaaagg aatgacacga gaagaagctt atgacttagt
     1681 tcaacctaag acagcttatt cctgggacaa tcaagtggat ttcaaaccac ttttagaaga
     1741 agacaccaaa gttacctctt gtcttacaca ggaagaaatt gatgaactat ttaatccgat
     1801 ttattacaca aaacgtgttg atgatatttt tgaaagacta ggattagaaa aataagaaaa
     1861 ttccattact ggttaacgtt tttttaacca gtaatggaat ttttattgat ttaaaaggaa
     1921 ttaatattaa aaaaacatac ttaaaatgag tttattatga taatatataa taagaagaaa
     1981 aatttaggag ttagttttta aaggaaaatg ttaaaacatt ttggtagtaa agtaagaaat
     2041 ttgagagtaa ctagaaatat tactcgcgaa gatttttgtg gtgatgaaac tgagttatct
     2101 gtaagacaat tagctaggat tgaaagtgga cagtcaatcc caaacttgac taaagctcac
     2161 tacatagcta aacaattgaa tgtaaaatta gatattttaa caggaggaga gagcttagaa
     2221 ttacctaaac ggtataaaga gttgaagtat ttgattttaa gaattccaac ttatgcagat
     2281 gctgaacgat taaaattaag agaatgtcag tttgatcata ttttcgaaga attctatgat
     2341 aatcttccag aagacgagtg cttagcaatt gacagtttac aagctaaatt tgaagtctat
     2401 caaacaggag acattaattt tggtgtggag gttttatgcg aatgctttga taaagtaaaa
     2461 tataaagaaa aatatactct taatgatttg attatcatag atttgttttt aacttgtgca
     2521 gttgtatcta aatttaacaa tagagcattt actaaagaag tatttcaaac tatatgtaaa
     2581 actttgataa gtcagaacca caaacttaca gcagaagatt tgttttggtt taatcatgta
     2641 ctcctgaatt gtgtatttgt tggactttgt ttaaatagtg aagagtgtct tgcagaaatg
     2701 ttagaagtta gccgacaaac gatggtgtca acacatgatt ttcataaaat gcctttatat
     2761 tttatgtacc aatggaaata cttcattaca attgacaatg atataaagtc tgcagaaaat
     2821 gcttaccaac aatcaattat gttttcaaaa atgattgatg acaagcatct aattaaaaaa
     2881 ttagaattgg agtggcaaga agatataaca ggacattgat gtcctgtgac tctgaggtct
     2941 aa
//
LOCUS       DATROD010000150         2899 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.152, whole genome shotgun sequence.
ACCESSION   DATROD010000150 DATROD010000000
VERSION     DATROD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.152"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..893
                     /locus_tag="VBC74_001917"
     CDS             <1..893
                     /locus_tag="VBC74_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEO6942821.1"
                     /translation="NLTVWHHLKHEIIKIPFSRLDILIHLRPTLMLFLPQITMQIYLS
                     LNKSMLGAMDSVVSAGYFDQSDKIIRILFTIVSAIGGVFLPRLSSLFSSGKEKQAKAL
                     LLKLVDLSNAISMLMIAGVVGVSSTFAVFFFGKGYEAVGPLMAVESLMIICISYGNAL
                     GTQYLLASRRTKAYTMSAVIGLVANVVLNILLIPILGAMGAIISTVITEFIVSLYQAI
                     SLRDVFTFKELTRGMLRYLIAATLSGAVLYYINTQMSVSLVNYVIQSLVAVTIYVGIV
                     FITKAPVIQLFRDFRKEHRT"
     gene            890..1738
                     /locus_tag="VBC74_001918"
     CDS             890..1738
                     /locus_tag="VBC74_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEO6942822.1"
                     /translation="MKYLAGIVTFNPNIERLDQNIRAIYPQVSHIYIVDNGSKNKEEI
                     SQLVADYNEEGHLTVDYLTENKGIAYALNCIGQFAVAQEFDWFLTLDQDSVVLGDLID
                     NYENYLLLPKVGMLSCLYQDMNREDLVMQEFDYKEIEECITSAALMKTSVFEETSGFA
                     EEMFIDFVDSEMNYRLSEMGYKTYQVNFIGLLHEIGHSSRVKKFGHVFHVLNHSPFRK
                     YYMIRNAIYIIKKYGKKKRHKYLVFMRNEFVRVLVAEEQKSKKLVAMIKGLKDGLLMK
                     VDKYGK"
     gene            1728..2876
                     /locus_tag="VBC74_001919"
     CDS             1728..2876
                     /locus_tag="VBC74_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEO6942823.1"
                     /translation="MENKVKTVAVFSGYYLPFLGGIERYTDKMTADLVKRGYRVVIVT
                     TNHDDLPIIDEDKGRKIYRLPTKNIVKQRYPIINKNREYNTLMKHVSDENIDFVICNT
                     RFQLTTLEGLSFAKKHRLPSIVLDHGSSHFSVNNRFLDFFGAIYEHLLTARVKHYRPD
                     FYAVSKRSVEWLKHFNIEAKGVIYNSVSESLGSDFADTAYLEKSADDIFITYAGRIIK
                     EKGIELLLEAFSMSQYSENVYLQIAGDGPELAHLKEKYQSKQINFLGKLNFEQTMSLM
                     AQTDIFVYPSMYPEGLPTSILEAGLLSSAIIATDRGGTVEVIDSPELGIIMEENTQSL
                     HESLDLLVKDKALREKLQQNIAKRIKEHFTWEKTVEKLDYIIQKELNL"
BASE COUNT          944 a          432 c          553 g          970 t
ORIGIN      
        1 gaaatttaac agtctggcat catttaaagc atgaaatcat caagatacca tttagtagat
       61 tagatatctt aatacatcta agaccaactt taatgttatt tttaccccaa atcaccatgc
      121 agatttatct ctctcttaac aagagtatgc tgggagcaat ggatagtgtg gtcagtgctg
      181 gatattttga tcaatctgat aaaattattc gtattttatt taccattgtt tcagcaatcg
      241 gaggagtttt cttaccaagg ctctccagtc tattttcttc aggaaaagaa aagcaagcta
      301 aggctttact tttgaaactt gttgatttaa gcaatgctat ctcaatgtta atgattgcgg
      361 gagtggtagg agtttcgtcg acatttgccg tcttcttttt tgggaaaggg tatgaagctg
      421 tcggtccgct aatggctgtt gagtctttga tgatcatttg catttcttat ggtaacgctt
      481 tagggacaca gtatctactg gcttcaagac gtacaaaagc ttacacaatg tctgcagtta
      541 ttggacttgt ggcaaatgtt gtgcttaata tacttttaat accaatttta ggtgctatgg
      601 gagctattat ctctacagtt attactgaat ttattgtctc cctctatcaa gctatctctt
      661 taagagatgt atttactttt aaagaattaa caagaggaat gctgcgctat ttaattgctg
      721 ctactcttag tggagctgta ctttattata tcaatactca aatgtcagta tcattggtaa
      781 attatgtcat tcaaagttta gtagcagtaa ctatctatgt cggtatcgtt tttataacca
      841 aagcaccagt cattcagtta tttagagatt ttagaaaaga gcatagaaca tgaaatattt
      901 agcgggtatt gtcaccttta accccaatat tgaacgctta gatcagaaca ttcgagcaat
      961 ttatcctcaa gtaagccata tctacatcgt agataatggc tcgaaaaata aggaagaaat
     1021 ttctcaatta gtggccgatt ataatgaaga ggggcaccta actgttgatt atttaacaga
     1081 aaataaagga attgcatatg ctttgaactg tatcggtcaa tttgcagtag cacaagaatt
     1141 tgattggttc ttgactttag atcaagattc ggttgtccta ggtgatttga tagataatta
     1201 tgaaaattac cttcttttac ctaaggtcgg tatgttatct tgtttgtatc aagatatgaa
     1261 ccgtgaagat ttagttatgc aagaatttga ctataaagaa attgaagagt gtattacttc
     1321 ggctgcttta atgaaaacga gcgtttttga agaaacatct ggctttgcag aagagatgtt
     1381 tattgatttt gttgactctg aaatgaatta tcgtctgtca gagatgggat ataagactta
     1441 tcaggttaat tttattggtc ttttacacga aattggacac tcttcacgag ttaaaaaatt
     1501 tggccatgtt tttcatgtat tgaatcattc accatttcga aaatattaca tgatacgcaa
     1561 tgcgatttat attataaaaa aatatggtaa gaagaagcgg cacaaatatt tagtatttat
     1621 gcgaaatgaa tttgttcgag tgttggtagc agaagagcaa aagagcaaaa aattagttgc
     1681 tatgattaaa ggcttaaaag atggcttatt gatgaaagtg gataaatatg gaaaataagg
     1741 taaaaacagt tgctgttttc tcaggatatt atctaccctt cctcggaggt attgaacgtt
     1801 atacagataa aatgacagca gatttggtta agcgtggcta ccgtgttgtt attgtaacaa
     1861 ctaatcatga tgatttaccg attatagatg aagacaaggg tagaaaaatt taccgtttac
     1921 cgaccaaaaa tattgtcaag cagcgctatc ctattattaa caaaaatcga gaatacaaca
     1981 cgctgatgaa acatgtttca gatgaaaata ttgattttgt gatttgtaat acacgttttc
     2041 agttaacaac gctagaaggg ctatcttttg caaagaagca ccgtttacct agtattgttt
     2101 tagaccatgg gtcaagtcat ttttctgtca ataatcgttt tttagatttc tttggagcta
     2161 tctatgaaca tcttctcaca gcgcgtgtta agcactatcg tccagatttt tatgcggtat
     2221 ctaagagaag cgttgagtgg ttaaaacatt ttaatataga agctaaagga gttatttata
     2281 attcggtatc tgaaagtcta ggttctgatt ttgctgacac ggcttacctt gaaaaatctg
     2341 ctgatgatat tttcatcact tatgctggta gaattattaa agaaaaaggc attgaattgc
     2401 tcttagaagc attttcgatg tcacagtact ctgaaaatgt ttatttacag attgcaggag
     2461 acggacctga attagcacat ttgaaagaga aatatcagtc caaacagatt aactttttgg
     2521 ggaaattaaa ctttgaacag actatgtctt taatggcaca gacagatatc tttgtttacc
     2581 catcaatgta tccagaggga ttaccaacct ctattttaga agctggtcta ctttcgagtg
     2641 ctattattgc aacagatcgc ggtggaacag ttgaagttat tgatagccct gagttaggaa
     2701 tcatcatgga agaaaatact cagtcactcc atgaatctct tgatttatta gtaaaagata
     2761 aggcattaag agagaaacta caacagaata ttgctaaaag aattaaagaa catttcacat
     2821 gggaaaaaac agttgaaaag ttggattata ttattcaaaa agaacttaat ctgtaataga
     2881 ttatgtcact aaattgacg
//
LOCUS       DATROD010000151         2855 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.153, whole genome shotgun sequence.
ACCESSION   DATROD010000151 DATROD010000000
VERSION     DATROD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.153"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            95..168
                     /locus_tag="VBC74_001920"
     tRNA            95..168
                     /locus_tag="VBC74_001920"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:129..131,aa:Met,seq:cat)
     gene            171..243
                     /locus_tag="VBC74_001921"
     tRNA            171..243
                     /locus_tag="VBC74_001921"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:204..206,aa:Phe,seq:gaa)
     gene            255..335
                     /locus_tag="VBC74_001922"
     tRNA            255..335
                     /locus_tag="VBC74_001922"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:289..291,aa:Tyr,seq:gta)
     gene            342..412
                     /locus_tag="VBC74_001923"
     tRNA            342..412
                     /locus_tag="VBC74_001923"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:374..376,aa:Trp,seq:cca)
     gene            427..499
                     /locus_tag="VBC74_001924"
     tRNA            427..499
                     /locus_tag="VBC74_001924"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:460..462,aa:His,seq:gtg)
     gene            506..577
                     /locus_tag="VBC74_001925"
     tRNA            506..577
                     /locus_tag="VBC74_001925"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:538..540,aa:Gln,seq:ttg)
     gene            588..671
                     /locus_tag="VBC74_001926"
     tRNA            588..671
                     /locus_tag="VBC74_001926"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Leu,seq:caa)
     gene            1047..1347
                     /locus_tag="VBC74_001927"
                     /pseudo
     CDS             1047..1347
                     /locus_tag="VBC74_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865753.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1587..1790
                     /locus_tag="VBC74_001928"
     CDS             1587..1790
                     /locus_tag="VBC74_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942824.1"
                     /translation="MAESANTVRSNSFVLEELTEFQREKLKNGESVKFNFSKDVKKGN
                     QITQGGSNLPNDGSVYLQPNKSL"
     gene            1941..2381
                     /locus_tag="VBC74_001929"
     CDS             1941..2381
                     /locus_tag="VBC74_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein BUG3"
                     /protein_id="HEO6942825.1"
                     /translation="MKKHIKSIIPIVLIGMILGGCQMNSEHKSQYNETKSSKQSEVKK
                     DKKMTKKEQLAYLKEHEQEIIDFVKSQNKKIESVQIDWNDVRWSKGGNGTPQGGGEGI
                     LLFGEINNDSESSWRVDIDIEKGRLDLKNMYLGQPIRIGGKLFE"
     gene            2374..2718
                     /locus_tag="VBC74_001930"
     CDS             2374..2718
                     /locus_tag="VBC74_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942826.1"
                     /translation="MSNWNLESFDNLFATLAESTYTKRPSNFTYNELNPKLLRQLNSG
                     SSVELDFSKSVKKGKKVIEGGQNLPNDGIVYLQPDKSLKSIDEKVEVRVPDVNGGYHS
                     EHCVTNSYQKGY"
BASE COUNT          999 a          418 c          589 g          849 t
ORIGIN      
        1 ttacccaagt ccggctgaag ggaacggtct tgaaaaccgt caggcgtgta aaagcgtgcg
       61 tgggttcgaa tcccacatcc tcctttaaag atatcgcggg atggagcagc taggtagctc
      121 gtcgggctca taacccgaag gtcgtaggtt caaatcctgc tcccgcaata ggctcggtag
      181 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cggttcgatt ccgtctcgcg
      241 ccatttatac cttaggagag atagcgaaga ggctaaacgc ggcggactgt aaatccgctc
      301 cttcgggttc gggggttcga atccctctct ctccatagta aacgggcata gtttaaaggt
      361 agaactaagg tctccaaaac cttcagtgtg ggttcgattc ctactgcccg tgttttttct
      421 aatatggcgg gtgtggtgaa gtggttaaca catcagattg tggctctgac attcgtgggt
      481 tcgattccca tcactcgcct tttcatgggg tatagccaag cggtaaggca agggactttg
      541 actccctcat gcgttggttc gaatccagct accccagttt taagatagcc ggcgtggcgg
      601 aattggcaga cgcgctggac tcaaaatcca gtgtcctcac ggacgtgccg gttcgacccc
      661 ggccgccggt atactttaaa agacaaggtt tctaaacctt gtcttttttt gttttgtact
      721 ggtaatttta agaaaaagtt actgaaaatt acttatattt gtttttcatt attgcaattg
      781 ttattgaagt tttaaaacaa gcagaaataa taaggaaatt tttcaggagt tagtggagac
      841 tcaacattta aatgaaattc aaacaggact cctaaatgtc ataggttctg aagtaataaa
      901 agtctttgtg aatgatagat aggtagtata aaataataag atttttcagt gtatatgtta
      961 cgactaaaaa attaaactcg tgaaaatttg ttatactaat attagtttta aaatactggt
     1021 aaataataat tatttttatt agatacttga catgggaagt ttattttcta agaaaagaga
     1081 gcatgatgaa aaaatatgta aaatggttaa ttcccatatc tattattggg atgttactag
     1141 caggttgtca aatgaatagt gaatctaaaa gtcagtcaaa cgaagtaaaa aatagcaaac
     1201 agtcagcagt aaagaaaggt aaaaaagtaa taaaaaaaga acaagtagct tatctcaaaa
     1261 agtatgaaaa agaattaact gaatatgtca aaacccaaaa tccagatatt agcgaaatta
     1321 agtatgattg gaatagtgtg cgtatagtgg aagctagtaa tggaacccct caaggcggag
     1381 agcagctatt attaatttgg ggttatgcaa atggcagtga attaaccaat tttactctaa
     1441 atttcacatt gggtgataat caaatgccta atattgactc agttggctca gataatttat
     1501 caactattga agattaataa taacagtaag aaaggtatat aatgggatgt tggaatctta
     1561 aggaatttga agatatattt tgaagtatgg cagaaagtgc taatacagtt cgatcgaatt
     1621 cttttgtttt agaagaatta actgaatttc aaagagaaaa gttaaaaaat ggggagtcag
     1681 ttaagtttaa tttttctaaa gatgttaaga aaggaaatca aatcacccaa ggtggctcaa
     1741 atctcccaaa tgatgggagt gtctatcttc agcctaataa gtccttataa actgtttatt
     1801 aaactgaaaa aattgaagtt ttcatagata aaattcttcg agtaaaatat tagagatgct
     1861 atttatattt cttaagctag taatttttta ttgtttattg gtttaatgat aatacaaata
     1921 taaaataggt ggtgcaaata atgaaaaaac atataaaaag tatcatacca atagttctta
     1981 ttggtatgat actaggaggc tgtcaaatga atagtgaaca taaaagtcag tataatgaaa
     2041 caaaaagtag caagcaatca gaagtgaaga aagataaaaa aatgacaaaa aaagaacaat
     2101 tagcttatct caaagagcat gaacaagaaa taattgattt tgtaaaatct cagaataaaa
     2161 agatagaatc tgtacaaatt gattggaatg atgttcgatg gagtaaaggg ggaaatggta
     2221 cacctcaagg aggaggagag gggattttac tttttgggga gattaataat gattctgaat
     2281 caagttggag agttgatatt gatatagaaa aaggacggct agacctaaaa aatatgtatt
     2341 taggacaacc tatacgaatt ggaggtaaat tatttgagta attggaactt agaaagtttt
     2401 gataacctat ttgctacttt agcagaatca acatacacaa aaagaccaag taactttaca
     2461 tataatgaat tgaatcctaa gttgctacga caactgaact caggtagttc tgttgaattg
     2521 gatttctcaa aatctgttaa gaaaggaaaa aaagttatcg aaggcggtca gaacctcccc
     2581 aatgatggta ttgtctacct gcaaccagat aagtccttaa aatctattga tgaaaaagta
     2641 gaggttcgcg ttcctgatgt caatggcggt tatcactctg agcattgtgt tactaattcc
     2701 tatcagaaag gttactgaaa gatgatgaag cagtggctct ataatcacta aaagctcatc
     2761 attgctcagt gtttaagtta acttctaaag ctcgaacctt gttaaatcaa ggtttgttta
     2821 cttttaaaag tgacatttta tttttctaaa aataa
//
LOCUS       DATROD010000152         2838 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.154, whole genome shotgun sequence.
ACCESSION   DATROD010000152 DATROD010000000
VERSION     DATROD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.154"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            63..932
                     /locus_tag="VBC74_001931"
     CDS             63..932
                     /locus_tag="VBC74_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEO6942827.1"
                     /translation="MKTTKVFSVLSLSVAVFMMTACQSNHSTKKADMSPDKVEQKTKF
                     KNISLSSNKLLAKENTMSVLWYQNSAEAKALYLQGYNVAKMKLDDWLQKPSEKPYSII
                     LDLDETVLDNSPYQAKNIKDGSSFTPESWDKWVQKKSAKAVAGAKEFLKYANEKGIKI
                     YYVSDRTDAQVDATKENLEKEGIPVQGKDHLLFLKKGMKSKESRRQAVQKDTNLIMLF
                     GDNLVDFADFSKSSSTDREQLLTKLQSEFGSKFIVFPNPMYGSWESAIYQGKHLDVQK
                     QLKERQKMLHSYD"
     gene            1067..2263
                     /locus_tag="VBC74_001932"
     CDS             1067..2263
                     /locus_tag="VBC74_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEO6942828.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGAKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            2526..>2838
                     /locus_tag="VBC74_001933"
     CDS             2526..>2838
                     /locus_tag="VBC74_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEO6942829.1"
                     /translation="MKTLKKLLSNYKFDIKKFKFGMRTFKTGLSVFLVLLVFHLFGWN
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VP"
BASE COUNT          949 a          415 c          572 g          902 t
ORIGIN      
        1 ggtttgattt tgaaagtaag cagtggtatg atttaattga aaaaatatag aggagaagtt
       61 ttatgaagac tacaaaagtt ttttcagttt tatcgttatc tgttgctgta tttatgatga
      121 cagcatgtca gtcaaaccac agtactaaaa aagctgatat gtctccagat aaggtggagc
      181 aaaagacgaa atttaaaaat atttctctaa gttctaataa actgttggct aaggaaaaca
      241 ctatgtctgt cctttggtat caaaattctg cagaagccaa ggctttatat ttacaaggtt
      301 ataatgttgc taaaatgaag ttagatgatt ggttacaaaa gcccagtgaa aaaccatatt
      361 caattatctt agatttagat gaaacagttt tagataatag cccatatcaa gcaaagaata
      421 ttaaagatgg ctctagtttc acgccagaga gttgggataa atgggtgcaa aagaaatcag
      481 ctaaggctgt tgcgggtgcc aaagaatttt tgaagtatgc taatgaaaag ggaataaaaa
      541 tttattatgt ctcagatcgt acagatgctc aagttgatgc gactaaagaa aatttagaga
      601 aggaaggtat acctgttcaa gggaaagacc acttgctttt ccttaaaaaa ggaatgaaat
      661 ctaaagagag tcgccgtcag gcagttcaaa aagataccaa tttaattatg ctttttggag
      721 ataatttagt tgattttgct gatttttcta aatcatctag tacagataga gaacaactac
      781 taactaaact tcaaagtgag tttggtagta aatttattgt tttcccaaat cctatgtacg
      841 gttcttggga aagtgctatt tatcaaggaa aacatctgga tgttcaaaaa caattgaaag
      901 aacgacaaaa aatgttgcat tcgtatgatt aataagtata cgaattctct taaaaacaac
      961 taggaaaatc tatgaaatct atttgtgaaa aagtaatcaa taattacaac tgttcacaaa
     1021 aatatgttat aataaataat atcaaattat ataaggagtt ttactaatgg ctaaattgac
     1081 tgttaaagac gttgatttga aaggtaaaaa agtcctcgtt cgtgttgact ttaatgtgcc
     1141 tttgaaagac ggcgttatca ctaacgacaa ccgtatcact gcggctcttc caacaatcaa
     1201 gtatatcatc gaacaaggtg gtcgtgctat cctcttctct caccttggac gtgttaaaga
     1261 agaagctgac aaagaaggaa aatcacttgc accggtagct gctgatttag ctgctaaact
     1321 tggtcaagat gttgtattcc caggtgttac tcgtggtgca aaattagaag aagcaatcaa
     1381 tgctttggaa gatggacaag ttcttttggt tgaaaacact cgttttgaag atgttgacgg
     1441 taagaaagaa tctaagaatg acgaagaact tggtaaatac tgggcttcac ttggagatgg
     1501 aatcttcgtt aacgatgcat ttggtacagc acaccgtgct catgcatcaa acgtaggtat
     1561 ttcagcaaac gttgaaaaag ctgtagctgg tttccttctt gaaaacgaaa ttgcttacat
     1621 ccaagaagca gttgaaactc cagaacgccc attcgtagct attcttggtg gctcaaaagt
     1681 ttctgataag attggtgtta tcgaaaacct tcttgaaaaa gctgataaag ttcttatcgg
     1741 tggtggtatg acttacacat tctacaaagc tcaaggtatc gaaatcggta actcacttgt
     1801 agaagaagac aaattggatg ttgctaaaga cctccttgaa aaatcaaacg gtaaattgat
     1861 cttgccagtt gactcaaaag aagcaaacgc atttgctggt tatactgaag ttcgcgacac
     1921 tgaaggtgaa gcagtttcag aagggttcct tggtcttgac atcggtccta aatcaatcgc
     1981 taaatttgat gaagcactta ctggtgctaa aacagttgta tggaacggac ctatgggtgt
     2041 ctttgaaaac cctgacttcc aagctggtac aatcggtgta atggacgcta tcgttaaaca
     2101 accaggcgtt aaatcaatca tcggtggtgg tgattcagca gcagctgcta tcaaccttgg
     2161 ccgtgctgac aaattctcat ggatctctac tggtggtgga gcaagcatgg aattgctcga
     2221 aggtaaagta ttaccaggtt tggcagcatt gactgaaaaa taatagtcat tataatttat
     2281 attaaaggac atgctgatgc atgtcttttt ttagttgata atgtagagtt attgaacttt
     2341 ccttgacttc atttatcatt tactagtttt tagatatagt ggtatttggt tgacactgca
     2401 attttcaaaa gtttatgacg atttgatata aattggttta taaaagatat actagtaata
     2461 aataaaacgt ttacaaaaag ggttttatgg attgatatgt tagaatatat agtgaaggga
     2521 taggtatgaa aacgttaaaa aaattattat ctaattacaa gtttgatatt aaaaagttta
     2581 aatttggaat gagaacattt aaaacaggct tatcggtctt tttagtttta ttggtttttc
     2641 atttatttgg ttggaacggg cttcaaattg gtgctctaac tgctgtattt agtttaagag
     2701 aagactttga taaaagcgtg cactttggtt tttctagaat tattggtaat agtataggtg
     2761 gcttactctc cttagtattt tttgcattca atgagatatt tcatcaagct ttttgggtca
     2821 ctttattaat tgttccta
//
LOCUS       DATROD010000153         2788 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.155, whole genome shotgun sequence.
ACCESSION   DATROD010000153 DATROD010000000
VERSION     DATROD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.155"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..376
                     /locus_tag="VBC74_001934"
     CDS             <1..376
                     /locus_tag="VBC74_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942830.1"
                     /translation="SQSNEVKNSKQSEVKKDKKMTKKEQLAYLKEHEQEIIDYVKLHN
                     NQIESVQFDWSSVKVEQSGNGTPQGGDYNLSLRGKFNHLQNSKLIVDFYLAHKNDIPN
                     IKSMGMLNKPYIHKNGIWHIYE"
     gene            440..2074
                     /locus_tag="VBC74_001935"
     CDS             440..2074
                     /locus_tag="VBC74_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEO6942831.1"
                     /translation="MSNWNLDNFDNLHSSLAESAYNSRPNSFPELFETDSVTEVKFSQ
                     PSEDNKGQITQGGTNLPNDGIVYLQPDKSLKSIDENVKVLIPDVNGGYHTEHYVTHSY
                     QKGVLTDDKAGFNAYYLSDTEKIDSTTKHTYLAIRGSDGIGLDTLNDWVSNNAMFAVS
                     NKYIPQAKLANKAMKEKIAELKGKAPGAVMDITGHSLGTIVSSQAVVNLSYAELENVG
                     QVVLFDGPDVSRSLEKMEGISAKKIQEAGKHVTYYVNPFDIVSMLNREKPWKDQFGKV
                     NVIVPSKYTGTFDEQSSHDFGAFQIDSSGNPLVASESYHPELLTSGQDLARLEKKFIK
                     NLLGEHYDSDKLGPVLKGALTGDLTRLIATLKACGISVTVNEAKKLYDNFQKDYKAII
                     SKAKKDGLEWDRKNIASFHSRIKSASGSQRILLRTQMLYMATQLAEADVSEKVSATKK
                     HISEAKDTIVEISTSTISSAEIMAMHLGQSEVDALVSDIKMSTVWNDSVETSDYEALD
                     HYKTKMTTFTTNLVTVAQNLTAQDEQLAGDIVTNLS"
     gene            2093..2365
                     /locus_tag="VBC74_001936"
     CDS             2093..2365
                     /locus_tag="VBC74_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942832.1"
                     /translation="MSKELKLPLNKMLKEQERIAIKAIVATAKSTITDTGTNLKATIE
                     TDAQDLKNYASTYNANITDGLEGQSAQAASDFLTQLPKPELDNPVN"
     gene            2375..2725
                     /locus_tag="VBC74_001937"
     CDS             2375..2725
                     /locus_tag="VBC74_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942833.1"
                     /translation="MVDTKKLQELDQEYDQNLRNIYRNREQLEDDFHLFMARTDSLKE
                     SVYQATLGQGWELPQEAHAHLYNMDDNKDTFISEFNEYMEKLEEKEIELRRVYNDRVD
                     ELYQKAKQNEAKKG"
BASE COUNT         1070 a          467 c          521 g          730 t
ORIGIN      
        1 aagtcagtca aacgaagtaa aaaatagcaa gcaatcagaa gtgaagaaag ataaaaaaat
       61 gacaaaaaaa gaacaattag cctatctcaa agagcatgag caagaaatca tagattatgt
      121 aaaattacat aacaaccaaa ttgagtccgt tcaattcgat tggtcaagtg taaaagtaga
      181 acaaagcggg aatggaactc cacaaggggg tgattataat ctttcactga gaggaaagtt
      241 taatcatcta caaaattcaa aattaatagt tgatttttat ttagctcata aaaatgatat
      301 cccaaatatc aaatcaatgg gaatgctaaa taagccatat atacataaaa atggtatttg
      361 gcacatttat gaatagcttg aatcatccta tataaagtaa tagaattttt accacaagca
      421 aggtagagga gaagaataat tgagtaattg gaacttagat aattttgata acttacatag
      481 ctctttagca gaaagtgcat ataatagtcg acctaattca tttcctgaat tatttgagac
      541 tgactcagta acagaagtaa aattttctca accctcagaa gataacaaag gtcaaatcac
      601 acagggtggc actaacctcc ccaatgatgg tattgtctac ctgcaaccag ataagtcctt
      661 aaaatctatt gatgaaaatg taaaggtcct cattccagat gtcaatggag gctatcacac
      721 tgagcattat gttactcatt cttatcaaaa aggtgtgctt actgacgata aggcaggttt
      781 taatgcttac tacctgtcag atactgaaaa aatagatagc actacaaagc acacttatct
      841 agccattcgt ggtagtgatg ggattggtct agatacacta aacgattggg ttagtaacaa
      901 tgcaatgttt gctgtctcta acaagtatat tccacaagcg aaattggcta ataaggcaat
      961 gaaagaaaaa attgccgagc taaaaggaaa agcacctgga gctgttatgg atattactgg
     1021 tcactcactc ggtacaattg tatcatctca agcagtagtt aatcttagct atgcagaatt
     1081 ggagaatgtt ggtcaagtag tccttttcga tggccctgat gtatcaagaa gcttggaaaa
     1141 aatggaaggc atttctgcta aaaaaatcca agaagctggg aagcatgtta cctactatgt
     1201 caatcccttt gatatagtta gtatgcttaa tcgtgaaaag ccatggaaag accaattcgg
     1261 gaaagtaaat gtcattgttc ctagtaaata tacgggtact tttgatgagc aatcttctca
     1321 cgattttggt gccttccaaa ttgatagtag cggaaatcct ttagttgctt ctgaaagcta
     1381 tcatccagaa ttattaacat ctggtcaaga cttagctaga ctcgaaaaaa aattcataaa
     1441 gaatctttta ggagagcatt atgattcgga taagctagga ccagtactca aaggcgctct
     1501 gactggggac ctgactcgac taattgcaac attaaaagct tgtggtatca gtgtcactgt
     1561 aaacgaagct aaaaaactgt acgataactt ccaaaaagac tacaaagcca taatttccaa
     1621 agctaaaaaa gatggcctcg agtgggatcg caaaaacatc gcaagcttcc atagtaggat
     1681 caaatcagca tcaggcagtc aacggatttt acttagaact caaatgctct atatggcaac
     1741 gcaattagca gaagcagatg tttcagaaaa agtctctgca acaaaaaaac atattagtga
     1801 agctaaggat acgattgtag aaatatcgac atccactatt tcatcagctg aaataatggc
     1861 tatgcacctt ggccaatcag aagttgatgc tctggtttca gatataaaaa tgtcaacagt
     1921 ttggaatgat agcgtagaga cttctgatta tgaagcactt gatcactata aaacaaaaat
     1981 gaccacattt acgaccaatt tagtaacagt tgcacaaaat ttaacagctc aagatgagca
     2041 actagctggt gatattgtca caaacttatc ctaaaataga gagattaaat gaatgtccaa
     2101 agaattgaaa ttgcccttga ataaaatgtt gaaagaacaa gaaagaatag ctattaaggc
     2161 aatagttgct acagcaaaaa gcactattac ggatactgga acaaatttaa aagcaaccat
     2221 agagacagat gcccaagacc taaaaaacta tgcatccacc tataacgcca atatcacgga
     2281 tggtcttgaa ggccagtcag ctcaagcagc atctgatttc ttgacacaac ttccaaaacc
     2341 agagttagat aatccagtca actaggaggc tagtatggta gatacaaaaa aactgcaaga
     2401 actcgatcaa gagtatgatc aaaatctcag aaacatttat cgtaacagag agcaactaga
     2461 ggatgacttt cacttgttta tggcaagaac agatagccta aaagagtctg tctatcaagc
     2521 aactttaggt caaggttggg aattacccca agaagctcat gcacacctat ataatatgga
     2581 tgataataag gatacattca tatcagaatt caatgaatat atggaaaagc ttgaagaaaa
     2641 ggaaatagag cttcgaagag tttataatga tagagtagat gaattgtatc agaaggctaa
     2701 gcaaaacgaa gcaaaaaaag gataaaaatt ttttcaagta atatatataa atatgaaaaa
     2761 taaagagtct tgcagtaaaa tgagtatt
//
LOCUS       DATROD010000154         2787 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.156, whole genome shotgun sequence.
ACCESSION   DATROD010000154 DATROD010000000
VERSION     DATROD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.156"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            93..728
                     /locus_tag="VBC74_001938"
     CDS             93..728
                     /locus_tag="VBC74_001938"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEO6942834.1"
                     /translation="MKKGLMISFEGPDGAGKTTVLEAVLPLLREKLSQDILTTREPGG
                     VTISEEIRHIILDVKHTQMDKKTELLLYMAARRQHLVEKVLPALEEGKIVLMDRFIDS
                     SVAYQGSGRGLDKSHIKWLNDYATDSHKPDLTLYFDVPSEVGLERIQKSVQREVNRLD
                     LEQLDMHQRVRQGYLELADSEPNRIVTIDASQQLDEVIAETFSIILDRINQ"
     gene            748..1611
                     /locus_tag="VBC74_001939"
     CDS             748..1611
                     /locus_tag="VBC74_001939"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEO6942835.1"
                     /translation="MDLKRTQPKLLEKFNTILQSDRMSHAYLFSGNFASLDMALYLAQ
                     SQFCEKRQSGLPCQECRACRLIANGEFSDVKIIEPQGQLIKIETIKELTKDFSRSGFE
                     GKSQVFIIKYCEKMHVNAANSLLKFIEEPQSSSYVILLTNDENNVLPTIKSRTQIFRF
                     PKQLDMLVHQAEQAGLLKSQASLLAQVADDPKHLEILLTNKKLLDYLNLSQQFVTTLA
                     KDRQTAYLEVSRLTSQVVDKNDQAFVFQWLTIMLAKEGQLYDLENTYRAQQMWKSNVS
                     FQNSLEYMVLS"
     gene            1641..1967
                     /gene="yabA"
                     /locus_tag="VBC74_001940"
     CDS             1641..1967
                     /gene="yabA"
                     /locus_tag="VBC74_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEO6942836.1"
                     /translation="MDKKDLFDAFDDFSQNLLVGLSEIETMKKQIQKLLEENTVLRIE
                     NGKLRERLSVIEAETETAVKNSKQGRELLEGIYNDGFHICNTFYGQRRENDEECAFCI
                     ELLYRD"
     gene            1967..>2787
                     /gene="rsmI"
                     /locus_tag="VBC74_001941"
     CDS             1967..>2787
                     /gene="rsmI"
                     /locus_tag="VBC74_001941"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEO6942837.1"
                     /translation="MEMQVQKSFKSNIHYGTLYLVPTPIGNLDDMTFRAIRILREVDF
                     ICAEDTRNTGLLLKHFDITTKQISFHEHNAYDKISGLIDLLKEGKSLAQVSDAGMPSI
                     SDPGHDLVKAAIEGDIPVVSIPGASAGITALIASGLAPQPHIFYGFLPRKKGQQITFF
                     ETKQDYPETQIFYESPFRVSDTLKHMKEIYGDRQVVLVRELTKLYEEYQRGTISQLLE
                     HIEKVPLKGECLIIVDGKRDTERVKDSSQQDPLVLVKEYIANGDKTNQAIKKVAK"
BASE COUNT          967 a          444 c          552 g          824 t
ORIGIN      
        1 ttaaaaatat cagacatgca caatcaagtt gtgggtgtct ttttttgata aattttgata
       61 gaatagtagt aattataaat aaaggatgta ccatgaaaaa aggactaatg atttcttttg
      121 agggcccaga tggtgcgggt aaaacaaccg ttttagaagc tgttttaccc ttgctcagag
      181 aaaaattatc acaagatatt ctcaccacca gagagcctgg tggcgtaact atttcagaag
      241 aaattcgtca tattatttta gatgttaaac acacgcaaat ggataaaaaa acagagctat
      301 tgctttacat ggctgctcga cgccaacatc tagtggaaaa ggtactccca gctctagaag
      361 aagggaaaat agtcctaatg gatcgtttta ttgatagttc tgttgcctat cagggatcag
      421 gaagaggatt agataagtca catataaaat ggcttaatga ctatgcgact gatagtcata
      481 aacctgattt aactttatat tttgatgttc cttctgaagt aggtttagag cgtattcaaa
      541 aaagtgttca aagggaggta aatcgtctag atctcgagca attagatatg catcaacgcg
      601 tccgtcaagg ttaccttgaa ttggcggatt cggagccaaa ccgcattgtg acgattgatg
      661 cctcccaaca gctagatgaa gtaatagctg agacattctc tattatttta gatcgtatca
      721 accaatgaat caggaagtac aaaggtcatg gatttaaagc gaacacaacc caaattgctg
      781 gaaaagttta atactatttt acagtcagat aggatgagtc atgcctatct tttttcggga
      841 aattttgcaa gtctagatat ggcactttat ttagcacaaa gtcagttctg tgaaaagcgc
      901 cagagtggtt taccatgtca agagtgtaga gcctgtcgtt taatcgctaa tggggaattt
      961 tcagatgtta aaatcataga accacagggg caacttatta agatagagac tataaaagag
     1021 ctgactaaag atttttcaag atcaggtttc gaagggaaat cacaggtctt tatcatcaaa
     1081 tattgcgaaa aaatgcatgt caatgcagcc aacagtcttt tgaaatttat tgaagaacct
     1141 caaagttcat cttatgtcat cttattgact aatgatgaaa acaatgtttt gccaacaatt
     1201 aagagtagaa cgcaaatttt tcgttttcca aaacaattgg acatgttggt tcatcaagct
     1261 gaacaagcag gattgctaaa atcacaagct agcttattag ctcaagtagc agacgatcct
     1321 aaacatttag aaatattgtt gactaacaaa aagttattag attacttgaa tttaagtcaa
     1381 cagtttgtga cgactttggc aaaagatagg caaactgctt atttagaagt atctcgcttg
     1441 acatcgcaag ttgtagataa aaatgatcag gcatttgttt ttcaatggct gacaataatg
     1501 ctggctaaag aaggccaact ttatgattta gaaaatactt atagagctca gcaaatgtgg
     1561 aagagcaatg ttagttttca aaatagttta gagtatatgg tgctttctta gaaaataata
     1621 cgcgcagaga ggaagagtga atggataaaa aagacttgtt tgatgccttt gatgattttt
     1681 cacaaaattt attagttggg ttgtctgaaa ttgagactat gaaaaaacaa atccaaaaat
     1741 tattggagga gaatactgtt ctacgcattg aaaatggtaa acttcgtgag cgtctgagcg
     1801 ttattgaagc agaaacagaa actgcagtta aaaattctaa acaaggaaga gaattactcg
     1861 aaggcattta caatgatggc tttcacattt gtaatacttt ctatggtcaa cgtcgtgaaa
     1921 atgacgaaga atgtgctttt tgtattgaat tattatatag agattgatgg aaatgcaagt
     1981 tcaaaaaagt tttaaatcaa atatacatta cggaacactc tatctagtcc caactccaat
     2041 tggtaatcta gatgatatga cttttcgtgc cattaggatt ttaagagaag ttgattttat
     2101 ttgtgcagag gatacacgaa atacgggact tttactcaag cactttgata ttactactaa
     2161 acaaattagt tttcacgaac acaatgctta cgataaaatc tctgggttaa ttgatttgtt
     2221 aaaagaaggg aaatctttag cccaagtatc tgatgcagga atgccctcta tttctgaccc
     2281 aggacatgac cttgtcaagg ctgctattga aggggatatc ccagttgtat ctataccagg
     2341 agctagcgct ggtattactg ctctcatcgc ttcaggttta gctccacaac ctcatatttt
     2401 ttatggcttc ttaccacgta agaaaggtca acaaataact ttctttgaaa caaagcaaga
     2461 ttaccctgaa acacaaatct tttatgagtc accgtttcga gtctctgata cgctaaaaca
     2521 catgaaagag atttacggag atcgccaagt tgttttagta cgcgaattga cgaaactcta
     2581 tgaagagtat caaagaggaa ccattagtca acttttagag catattgaaa aggtccctct
     2641 caaaggtgaa tgcttaatta ttgttgatgg taagagagat accgagcgag tgaaagacag
     2701 tagccaacaa gatccactag tattagtaaa agaatatatc gctaatggtg ataaaactaa
     2761 tcaagcgata aaaaaagtag caaaaga
//
LOCUS       DATROD010000155         2728 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.157, whole genome shotgun sequence.
ACCESSION   DATROD010000155 DATROD010000000
VERSION     DATROD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.157"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(77..1459)
                     /locus_tag="VBC74_001942"
     CDS             complement(77..1459)
                     /locus_tag="VBC74_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEO6942838.1"
                     /translation="MLTTKENFKGNNKLLVGIVLAVLSFWLFAQSILNMGPDVQSSLG
                     ISSGAMDIGVSSTALFSGLFIVVTGGLADKLGRVKFTFIGLCLNIIGSLLIVLANGAV
                     LFIMGRIFQGLAAAFIMPSTMALVKTYYDGKDRQRAVSFWSIGSWGGSGLCSYFGGAV
                     ASTLGWRYVFIFSIIASVVSFLLILGTPESKNVGQKTHFDYLGLIIFIISMLSLNIGI
                     SMAQEHGLMNVIPLSLFTVMLIGFVLFYYVETRKSNSFIDFHLFENRFYLGATISNFL
                     LNAVAGTLIVINTYMQQGRQLTPKVAGEMSLGYLVCVLIAIRVGEKILQRFGARKPML
                     LGAMSTFVGIFLMTLVNIQGPLYLVLVFVGYALFGTGLGIYATPSTDTAISSIPNEKV
                     GSASGIYKMASSLGGAIGVATSIAIYHAFSGNADFHKAALCGLILNLVFCSLSILSIL
                     FVIPKKIEDK"
     gene            complement(1467..2681)
                     /locus_tag="VBC74_001943"
     CDS             complement(1467..2681)
                     /locus_tag="VBC74_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEO6942839.1"
                     /translation="MANASLRHQLFEKLDQKCDQMVAIRRYLHENPELSFKETKTAAY
                     ISDFYKGKDCHVQTQFGGMNGVVVDIYGDKATDKPIKHIALRADFDALPIQEETGLSF
                     ASKTAGVMHACGHDAHTAYLLILAESLIELKSEFSGHIRILHQPAEEVPPGGAKAMIE
                     AGCLDGIDAVLGIHVMSTMEEGTVQYHAGPIQTGRATFKVILQGKGGHGSMPHRANDT
                     IVAASSFVMAAQTIVSRRVNPFDTAVVTIGSFDGKGSANVIKDSVTLEGDVRVMSEET
                     RGVVEEEFKRILDGIAQTYGVSYQLDYQNDYPVLVNNSEVTQKVANSLKSVAIKEILD
                     VIDCDPQTPSEDFAYYAQTIPACFFYVGAHEEGQPYYPHHHPKFQIAESSLMVSAKSM
                     ATAALAMLVEGE"
BASE COUNT          929 a          576 c          421 g          802 t
ORIGIN      
        1 tattttaaat ttctttccca attcctttta cattattaca acaatacttg agattttgaa
       61 tctcaagtat tgttatttat ttatcctcaa ttttcttagg gataacaaaa agaattgata
      121 agatcgatag actacaaaaa actaggttta aaattaatcc acacaaagct gctttgtgaa
      181 aatctgcatt accagaaaaa gcatgataaa tcgcaattga agttgctaca ccaattgcac
      241 cacctagtga acttgccatt ttgtaaattc cagatgcaga acctactttt tcattaggaa
      301 tactagaaat agctgtatct gttgatggtg tcgcatagat ccccaagcct gttccaaata
      361 aagcataacc aacaaagact aacacaagat acaaaggtcc ttgaatattg accaaagtca
      421 ttaaaaatat cccaacaaat gtactcatag ctcccaaaag cattggctta cgggcaccaa
      481 aacgttgtaa aattttctca ccaactcgga tagcaattag tacacaaacc aaatagccta
      541 aagacatttc acctgcaacc tttggtgtta gctggcgacc ttgttgcatg taagtattaa
      601 taacaatcag agttcctgca actgcattca gtaagaaatt tgatattgtc gcacctaagt
      661 aaaatcgatt ttcaaacaga tgaaagtcaa taaaactatt agatttacgt gtttcaacat
      721 aataaaacag aacaaatcca ataagcatca ctgtaaaaag actcaatgga ataacgttca
      781 tcaatccatg ttcctgagcc atagatatcc caatatttaa cgataacatt gaaataataa
      841 aaataattaa accaaggtaa tcaaagtgag ttttttgtcc aacattttta ctttctgggg
      901 ttcctaaaat taatagaaaa ctaactactg atgcgatgat tgaaaagata aagacataac
      961 gccaacctag agtacttgcc accgctccac caaaataaga acatagacct gagccacccc
     1021 acgatccgat tgaccagaaa ctaactgcac gttgtctatc tttaccatca tagtacgttt
     1081 taaccaaggc cattgttgat ggcataataa aagctgctgc caatccttga aaaatacgtc
     1141 ccataataaa tagcactgct ccattagcta aaacaattag tagagaacca ataatattta
     1201 agcaaagacc aataaaagta aatttaactc gaccaagttt atctgctaaa ccaccagtta
     1261 caacaataaa taaacctgaa aacaatgccg tacttgagac accaatatcc attgcaccag
     1321 atgaaatacc taatgatgac tgaacatctg gtcccatgtt taaaatagac tgtgcaaata
     1381 accaaaatga tagtacagcg agtacaattc ccaccaataa tttgttattt cctttgaaat
     1441 tttcctttgt cgttaacata tgttatctat tctccttcta caagcatagc aagcgctgct
     1501 gttgccattg atttagcaga taccatcaat gatgattctg caatttggaa ttttggatga
     1561 tgatgtgggt aatatggttg cccctcttca tgtgctccaa catagaagaa gcaagctggg
     1621 attgtttgag cataataggc aaaatcttca gacggcgttt gagggtcgca atcaatgaca
     1681 tctaaaattt cttttatagc aacagatttt aaactatttg caactttctg tgtcacttca
     1741 ctgttattaa ctaaaacagg ataatcattc tgatagtcta actgataact aacaccataa
     1801 gtttgtgcta ttccatctag aatacgctta aattcctcct caacaacacc tcgtgtctct
     1861 tcagacatca cgcgcacatc accttctaag gttacactat ctttgatcac attagcagaa
     1921 cctttaccat caaaagatcc gatagtaact actgctgtgt caaatggatt aactcgacga
     1981 ctgacaatcg tttgcgctgc cataacaaaa ctagaagctg caacaattgt atcatttgca
     2041 cgatgtggca tggaaccatg tccaccctta ccttgcaaaa taactttaaa agtggcacgc
     2101 cctgtttgaa tgggacctgc gtgatattgt accgttccct cttccatcgt tgacataaca
     2161 tggataccaa ggacagcatc aatgccatct aaacatcctg cttcaatcat tgcttttgct
     2221 ccgcctggtg gcacctcttc agccggttga tgtaagatac ggatatgacc tgaaaattct
     2281 gattttaatt caatcaagct ctcagccaaa atcaataaat aagctgtatg ggcatcatgt
     2341 ccacaagcat gcatgacgcc cgctgttttt gaagcaaaag aaagccctgt ctcttcctga
     2401 attggaagag catcaaagtc ggctctcaaa gcaatgtgtt tgatgggctt atctgtcgct
     2461 ttatcaccat agatatcaac tacgacgcca ttcataccac caaattgtgt ctgaacatga
     2521 cagtccttac ctttataaaa atctgaaata taagctgctg tttttgtctc tttaaatgat
     2581 aattcgggat tttcatgtaa atagcgacga atagcaacca tttgatcaca tttttggtca
     2641 agtttttcaa ataattgatg tcgcaaagat gcattggcca tttgctttcc tccttaaaat
     2701 atgaacagtt aaaataaggc tattattc
//
LOCUS       DATROD010000156         2661 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.158, whole genome shotgun sequence.
ACCESSION   DATROD010000156 DATROD010000000
VERSION     DATROD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.158"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            129..197
                     /gene="shp2"
                     /locus_tag="VBC74_001944"
     CDS             129..197
                     /gene="shp2"
                     /locus_tag="VBC74_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEO6942840.1"
                     /translation="MKKINKALLFTLIMDILIIVGG"
     gene            258..1847
                     /locus_tag="VBC74_001945"
     CDS             258..1847
                     /locus_tag="VBC74_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase"
                     /protein_id="HEO6942841.1"
                     /translation="MKIKKIISGFAAALIISSLSTINYEVKADDTTSEYHYISKQNNE
                     KQLISYIKEQHRLLNQFVVDNVNSFTQLNANPTIEQLNRAITLFKQKDEQLFNQVKAG
                     HLSPSNYNAIVNQRNVINQTVQNLIDQNHNKIQTSQNKAAQLVGQRNQVVNKIQAILA
                     TVNYNSVNSIQEAENLFHSLRNQIEPLVAEVNNYKAAMAILQQEVDALSTAAIETETS
                     KLATLKVSENTSVPANKVEEKTTQSEASGNKQEVTKSEEKQATSDAKASQPESANIAD
                     YDSLKEVLRNNISNQVPHISVQMEFKTQEQVDEYQKNLGSIIREIGDTLGTATEFNAK
                     SNISTYTLGGQIQRIIVKSDITITYTLKGDMVGLHKEYKQFVDSFVKENITNKNITSD
                     YEKAKVIHDHLVNNYTYATEELATTRETASGISIHAPEALYKDKRGVCQAFAVMFKDM
                     AAAGLSVWYVTGQAGGGNHAWNIVTINGVKYYVDTTWDNNIKSNKYFLVGKTIMDADH
                     LLDSQYNALAKDIPADRHQGA"
     gene            1883..2059
                     /locus_tag="VBC74_001946"
     CDS             1883..2059
                     /locus_tag="VBC74_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942842.1"
                     /translation="MSKKTDSNYLHSNCSDKKIWSDEAPLQQETIPKDRDCVIKPVIT
                     MTVEHVEIPLDDQS"
     gene            complement(2110..2304)
                     /locus_tag="VBC74_001947"
     CDS             complement(2110..2304)
                     /locus_tag="VBC74_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942843.1"
                     /translation="MLFLMIKPDYIRYFKVIDDMHDTMYQQTVFHVQLQAWKYTMKSI
                     DQLVVDDLNHISNEKTTLVK"
     gene            2303..2497
                     /locus_tag="VBC74_001948"
     CDS             2303..2497
                     /locus_tag="VBC74_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942844.1"
                     /translation="MIMSGQRATSTIQSDINIFHHIEAICISKSNSIGLPAIQNHLGQ
                     LIQRERLGLFFSITYSKAPT"
BASE COUNT          961 a          488 c          423 g          789 t
ORIGIN      
        1 ttacgtaatc ttcttagtgt ttttcctaat tctttttcca tttttcccac tttcacaaca
       61 atttaaaaga ccctttaaat ccattatata atacaagtac aaaatagaaa atcagaaagg
      121 agatagtcat gaaaaaaatt aacaaagctt tgttgttcac attaatcatg gatatcctaa
      181 ttatcgttgg tggataaaac tccccacaaa tggttgttat ggaatcccat tttatttagt
      241 ttataacagg agaactaatg aagattaaaa aaattattag tggctttgcc gcagctttaa
      301 ttatcagttc actatcaact attaactatg aggttaaagc tgatgacacc accagtgagt
      361 atcactatat cagtaagcaa aataatgaaa agcagcttat tagttacatc aaggaacaac
      421 atcgtttgct caatcaattt gttgttgata atgtcaactc attcactcaa ctaaatgcta
      481 atccaactat tgaacagtta aatagagcta taacattatt taaacaaaaa gatgagcaat
      541 tatttaacca ggtgaaagct ggtcatctct ctcccagtaa ctataatgct atcgttaatc
      601 aacgtaatgt cattaaccaa actgttcaaa atctgattga ccaaaatcat aataagattc
      661 agacaagtca aaataaagca gctcagctcg tggggcaacg taatcaggtt gttaacaaaa
      721 ttcaagctat tttagcaact gtaaactaca actctgtgaa ttctatacaa gaagctgaaa
      781 atttatttca ttcactcaga aatcaaattg aacctcttgt agctgaagtt aataattaca
      841 aagctgctat ggcaatcctt caacaagaag tagatgccct atcaacagcg gctattgaaa
      901 ctgagacttc taaacttgct actctcaaag ttagcgaaaa tacttctgtt cctgcaaaca
      961 aagtagaaga aaaaactact caatcagaag cgtcaggcaa taaacaagaa gtaactaaga
     1021 gtgaggaaaa acaggctacc tctgatgcaa aggcatcaca gcctgagtca gctaatattg
     1081 ccgattacga tagtttaaaa gaagttttac gaaataatat tagcaaccaa gtaccacaca
     1141 tcagtgttca aatggagttt aaaactcaag aacaagttga cgaataccaa aaaaatctcg
     1201 gaagcatcat ccgggaaatt ggagatacac ttggaacagc aactgaattc aatgccaaaa
     1261 gtaacattag cacttatact cttggtggac aaatccaacg cattattgta aaaagcgaca
     1321 tcacaatcac ctatactctt aaaggtgaca tggtaggatt acataaagaa tataaacagt
     1381 ttgtagattc ttttgtcaaa gaaaatatta ctaacaaaaa tatcacaagt gattatgaaa
     1441 aagctaaagt aattcatgac cacttggtta ataattacac ttacgcgact gaagaactgg
     1501 caaccactcg tgaaactgct agtggtatca gtatccatgc tcctgaagca ctctacaaag
     1561 ataaacgtgg tgtttgtcaa gcctttgcag taatgtttaa agatatggct gctgctggct
     1621 tatcagtatg gtatgtaact ggtcaagctg gaggtggaaa tcacgcttgg aacattgtta
     1681 ctattaatgg cgttaaatat tatgttgata caacatggga taataatata aaaagcaata
     1741 aatatttcct tgttggtaaa acaataatgg atgctgatca tcttttggat agtcaataca
     1801 atgcattagc taaagatatt ccagctgatc gccatcaagg tgcataactc aaaaattgta
     1861 ttgagcctgg aaaggatatg aaatgtctaa aaagactgat tcaaattatc tacattccaa
     1921 ttgttctgac aagaaaattt ggagcgatga ggcgcctctg caacaggaga ctatccctaa
     1981 agacagagat tgtgttatca aacctgtcat tactatgaca gttgaacatg tggaaatccc
     2041 attagatgac caatcttagc tctctcctaa aataaaaagg aacactgcca tttttgacag
     2101 tgttcctttt tatttaacca aagtggtttt ctcattactg atatgattca aatcatctac
     2161 aactaattga tcaatcgatt tcatggtgta tttccaagct tgcagttgaa catgaaaaac
     2221 agtctgttgg tacatggtat catgcatatc gtcaatgact ttgaaatacc ttatataatc
     2281 tggtttaatc attaggaaca gcatgatcat gagtggtcaa agagctacca gcaccatcca
     2341 gagcgatata aatatctttc atcatataga agctatttgt ataagtaaat ctaacagtat
     2401 tgggctcccc gcaatacaaa atcacttggg acagctgatc caaagagaac gtctgggtct
     2461 tttcttctcg ataacctatt ccaaagctcc cacttgaagt acttactgaa tctgttgcaa
     2521 cagaaaaaca atccctacta ctagaataat agcacctcta actaataaat gactcattcc
     2581 aacattcaca tcttttaaca tcatgttcct ttcattattt tttatttagg aattcgtcat
     2641 actatatttt ctcatattta t
//
LOCUS       DATROD010000157         2595 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.159, whole genome shotgun sequence.
ACCESSION   DATROD010000157 DATROD010000000
VERSION     DATROD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.159"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            3..860
                     /locus_tag="VBC74_001949"
     CDS             3..860
                     /locus_tag="VBC74_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEO6942845.1"
                     /translation="MKNISPLRYPGGKSQVYDFVKELVNLNETNTYIEPYMGGMGIAL
                     KLLVNNDVKQIMVNDYDRSIYAFWYSVLNHTNQLIDKIESTPITIEEWKKQRKIQQGK
                     ETCHDLLTLGFSTLFLNRTNRSGIIKAGVIGGLKQDGNYKLDCRFNKEKTIKKIRLIA
                     SYKKQIKLYNIDAEKFIRLNITKTKKSFTFFDPPYYSKGPGLYTNFYNHENHLSLSNT
                     IKNYMSNKNWILTYDLSEEIFQMYKEFKYEKYYLNYSVTKPSKGIEYIFYSNGLTVPE
                     NTINIKKAN"
     gene            980..2122
                     /locus_tag="VBC74_001950"
     CDS             980..2122
                     /locus_tag="VBC74_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6942846.1"
                     /translation="MWSEKHKSGKVNFIERYKDPYTSRWKRTSVLMEKDTPRIRKEAQ
                     RILDAKIADIVRKLQTSDMLFTDLLDQWWGFYQQEIKRSSIASLRGNIRDIRESFGID
                     IKVVNIDPKYVQTYLNNLDCSRNKKERNKSMLNLIFDYAVSLDIIKDNPARRAKLPRI
                     KKTLEDWKKIEEKYLEEEEIKRLLKELFRRPSTYRLGLLSEFMSLNGCRIGEAISIEP
                     DNIDFKNKTLQLHGTYDRTNGYMNGEKTSPKTLASYRETIMTKREMEILQEMEFINEL
                     EKNTNKRYRDMGYIFTTKNGIPVQINSFNLALKKANKRLKHPINKNITSHIFRHTLVS
                     RLAENNVSLKAIMDRVGHADAKTTVQIYTHITKKMKSNVADIMENY"
     gene            complement(2211..2486)
                     /locus_tag="VBC74_001951"
     CDS             complement(2211..2486)
                     /locus_tag="VBC74_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEO6942847.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFAAVADYLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
BASE COUNT         1015 a          426 c          413 g          741 t
ORIGIN      
        1 gattgaaaaa tataagcccg ttgagatacc ctggaggtaa gtctcaagtt tatgattttg
       61 ttaaagaact tgttaacctt aatgaaacaa atacatatat agaaccgtat atgggaggta
      121 tgggaattgc attaaagcta cttgttaata atgatgtaaa acaaatcatg gtaaatgact
      181 atgatcgttc aatctatgct ttctggtact ctgtcttaaa tcatacaaat cagttaattg
      241 ataaaataga gtccacacct atcacaattg aagaatggaa aaaacaaaga aaaattcaac
      301 agggtaaaga gacatgtcat gatttattaa ctcttggatt ttcaactcta tttcttaatc
      361 gaacaaatcg ttctggaata ataaaagcag gtgtcatcgg tggactaaaa caagatggaa
      421 attataaatt agattgtcgt ttcaacaaag aaaaaactat aaaaaaaata agattgattg
      481 catcttataa aaaacaaatt aaactttata atatagatgc tgaaaagttc attcgtttaa
      541 atatcaccaa aacaaaaaaa tctttcactt tttttgaccc tccatactac tcaaaaggac
      601 ctggattata tacaaacttt tacaatcatg aaaatcatct aagtttatcc aacacaatta
      661 agaattatat gtcaaataaa aattggattc tcacgtatga tttatctgaa gaaatttttc
      721 aaatgtataa agaatttaag tatgaaaaat attacctgaa ctattcagta acaaaaccaa
      781 gtaaaggaat agagtatatt ttctactcta atggattaac agtcccagaa aatacaataa
      841 atataaaaaa agcaaattaa aaaaagacct acacagcgcc ggcaagcaaa cgtgtaggtc
      901 aagttggtaa tagtaaaaac ctgctttaag taggctcttt actgtaccca ttttaacaaa
      961 aaaaatgagg taaaaaacaa tgtggtcaga aaaacataaa agcggaaaag tgaattttat
     1021 cgaaagatat aaagatccgt atacaagcag atggaaacgt acatcagtac tgatggaaaa
     1081 agatactcct cgaattagaa aagaagctca aagaatatta gatgctaaaa tagctgacat
     1141 cgtaagaaag ttacaaacct ccgacatgct atttacagac cttcttgacc aatggtgggg
     1201 attttaccaa caagagataa aacgctcctc tatagcctct ctgagaggca atataagaga
     1261 tattagagaa agctttggaa ttgatattaa ggttgtcaat atcgacccta aatatgtcca
     1321 aacctacttg aataacttag attgctccag gaacaaaaaa gagcgaaaca agtccatgct
     1381 taatcttatc tttgattatg ctgttagtct agatattatc aaagataatc ctgcaaggcg
     1441 tgctaaactt ccgcggataa agaagacttt ggaagattgg aaaaagattg aagaaaaata
     1501 tcttgaagaa gaagaaatca aacgactatt aaaagaatta tttagaagac ctagcactta
     1561 ccgtctggga ttactatcag aatttatgag tcttaacgga tgccgtatcg gtgaggctat
     1621 tagcatagag ccggataata ttgattttaa aaacaaaact ttacagttgc atggtactta
     1681 tgatcgtacc aatggttaca tgaatggaga aaagacctct ccaaaaacgc ttgcgtccta
     1741 tcgcgaaacc attatgacta agcgagaaat ggaaatttta caagagatgg aatttattaa
     1801 tgagctcgaa aaaaatacaa ataaacgata tagagacatg ggatatatct ttacaaccaa
     1861 gaatggtatt cctgtacaaa taaattcctt taatttagca ttgaaaaaag caaacaaaag
     1921 acttaagcat ccaatcaata aaaatatcac tagtcacatc ttccgccaca cattagtcag
     1981 tcgtcttgct gaaaataacg tatctttaaa ggcaatcatg gatcgtgtag gacacgctga
     2041 cgcaaaaact acagtacaaa tttatacaca cataaccaag aaaatgaaat caaatgtagc
     2101 tgatatcatg gaaaactact aatttcatgc cccaaatatg ccccaaaaat aaaaaaaaga
     2161 accttgtcgc tatcaataaa cattgatatg acaaggtttt tctagacgaa ttatttaaca
     2221 gcgtctttaa gagctttacc agctttaaat gctggaactt ttgatgctgc aatttcgatt
     2281 tctgcaccag tttgtgggtt acgaccttta cgagctgcac gttcacgaac ttcaaagtta
     2341 ccaaaaccga ttaattgtac tttttcacct tcagcaaggt aatctgctac agctgcaaaa
     2401 actgcatcta ctgctgctgc tgaatctttt ttagttaatt ctgtagcttc tgctactttt
     2461 gcaattaaat cttgtttgtt agccatgtta acaaatcctc caaataaatt ctaaacttat
     2521 acaacataag ttttaacaga ttatatgata tctaaaaaaa ggcttaaggt caagtattta
     2581 acgtcatttt agggc
//
LOCUS       DATROD010000158         2547 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.160, whole genome shotgun sequence.
ACCESSION   DATROD010000158 DATROD010000000
VERSION     DATROD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.160"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            114..701
                     /locus_tag="VBC74_001952"
     CDS             114..701
                     /locus_tag="VBC74_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942848.1"
                     /translation="MTYSYSEYDLNRLNQGENVYSVNPNYVKQNGGKIITANPKKSNE
                     TNLIVTKTGKAFKVLKTSPDDMSGYQGMAVAPIIKGKVDYNSVAVISAATDSSNYKDL
                     IGAVSSAQPHQSSTQLKSADKFLKDVQSHDKWTVTQLSGYSQSAYMLKLGAKYHIPTT
                     VFNGWFRYSTLNEDEKNSWLSILNILLIFDIKRIM"
     gene            647..1327
                     /locus_tag="VBC74_001953"
     CDS             647..1327
                     /locus_tag="VBC74_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942849.1"
                     /translation="MAKHPEYFANFRHKEDNVTWWNDFNKLDDKGYGTVKWVNGKSHK
                     IESWKFTDDGQLKDEKGNIVNPKSPAVQSVLYEEVHFQKAKAKLKKSGGKLSHSEKVY
                     LDSEQAIFIANGLTTASQTASDDIKKNAELAKEKASELFAKTKVMPPGITDLSPEELA
                     DAYSAGGVREDTIVTPIETFFDEKVTNAQEITTSYTNLQKQIESGVQKLLEEDSKLAG
                     EFKEWSQY"
     gene            1312..1698
                     /locus_tag="VBC74_001954"
     CDS             1312..1698
                     /locus_tag="VBC74_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942850.1"
                     /translation="MVTILDQNENKINSLKINLGKLEDNYHRENMEIEDKLYSLEDKK
                     NELETYLQEVYEETSHFLRQNDSDGSTFMSLNRIIESYQMELIDEYENYKQTIIVQEE
                     NSRTQFLKDRVKLENDISSLEGRKYY"
     gene            1732..2013
                     /locus_tag="VBC74_001955"
     CDS             1732..2013
                     /locus_tag="VBC74_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942851.1"
                     /translation="MSLIGAVLKGTKEDKEFKKQIQKALVRFVHAPEIVEEADSIKTT
                     YSEKIDDISETAKSSIKTIKNQIDTGLVGQFWEKVKAQVDLNKGSYDDL"
BASE COUNT         1025 a          336 c          451 g          735 t
ORIGIN      
        1 aatcgcagtg gatcaataaa tgaaaaagaa catttatttt taagtaaaaa atatggaaga
       61 acaaaaaatc atattttagt tgagttttct acaggagaaa aggaaaaaag ataatgacat
      121 attcttatag cgagtatgat ttaaatagat tgaatcaagg tgagaacgta tatagtgtaa
      181 atccaaacta tgtgaaacaa aatggcggaa aaataataac agctaatcca aaaaaatcta
      241 atgaaacaaa tttaatagta acaaagacgg gtaaagcgtt taaggtatta aaaacttctc
      301 ctgacgatat gtctggctat cagggtatgg cagttgcacc aattatcaag ggtaaagtag
      361 attataacag tgttgcagtt atttccgccg caacggattc aagtaattat aaagatttaa
      421 ttggcgcagt ttcatctgct caaccacatc aaagttcgac tcagttaaaa tcagctgata
      481 aatttttaaa agacgtacag tcacatgaca aatggactgt gactcagctt tcaggctatt
      541 ctcaatctgc ttatatgcta aaattaggtg ctaaatatca tatcccgact acagtattta
      601 atggatggtt taggtatagc actttaaatg aagatgaaaa aaattcatgg ctaagcatcc
      661 tgaatatttt gctaattttc gacataaaga ggataatgta acttggtgga acgattttaa
      721 taaactagat gataaaggtt atggtaccgt gaaatgggta aatggtaaaa gtcataaaat
      781 agagagttgg aaatttactg atgatggtca gttaaaagat gaaaaaggta acatcgtaaa
      841 tcctaagtca ccagctgttc agtctgttct ttatgaagaa gttcattttc aaaaagctaa
      901 agctaaatta aaaaaatctg gaggcaaact tagtcatagt gaaaaagtat atttagattc
      961 tgaacaggct atatttattg ctaacggttt aaccacggca tctcaaacag cttctgatga
     1021 tatcaagaag aatgctgaat tagctaaaga aaaagcatca gaactatttg caaaaactaa
     1081 agtgatgcct cctggtataa cagatttaag tccagaagaa ttagctgatg catatagtgc
     1141 gggtggagtt agagaggaca ccatagtaac accaattgaa actttctttg atgaaaaagt
     1201 tactaatgca caagagatca caacttcata tactaatctt caaaaacaaa ttgaatctgg
     1261 tgtccaaaaa ttgctagaag aggactccaa attagcagga gaatttaagg aatggtcaca
     1321 atactagatc aaaatgaaaa caaaattaat agtctaaaaa taaacctagg gaaattggaa
     1381 gataattatc atcgcgaaaa tatggagata gaagataaat tatatagcct tgaggataaa
     1441 aagaacgagt tagagactta tttacaagaa gtctatgaag agacaagtca ctttttgaga
     1501 caaaatgatt cagatggttc gacttttatg agtttaaatc gaataattga aagttatcaa
     1561 atggaattaa ttgatgaata tgaaaactat aaacagacta tcattgtcca agaggaaaat
     1621 agtcggacac agtttctaaa agaccgagta aaattagaaa atgacatttc tagtttagaa
     1681 ggaagaaaat actactaatt tagatggact ataaagtgaa agaggcaata aatgagttta
     1741 ataggcgctg tattgaaagg aaccaaagaa gacaaagaat tcaaaaagca aattcaaaaa
     1801 gctttagtaa ggtttgtcca tgccccggaa atagtagaag aagctgattc tattaagacg
     1861 acttatagtg aaaagattga tgatatttct gagactgcta aatcatcaat aaaaacgata
     1921 aaaaaccaaa ttgacactgg acttgtagga caattttggg aaaaagtaaa ggcacaggta
     1981 gatttaaata aaggtagtta tgatgattta taatcgagtg aaaatgttga ggaagcttac
     2041 tatcctgata gttattatag atatgaaaat aatcaagttg gattagaaga cataacattt
     2101 tacatgaagg ataacaaagt ttattattta cattttggta aagcaataga taaaaatgat
     2161 aagtatctca atcaaattat aaataattaa tttctttatt ttataagccc cgaaaacaaa
     2221 ttttttgata accttttgat aacccctccg cgaacatatt gtaatacata gtgcactttt
     2281 gggatataaa ttgttgattt ttagctagtt tataccatat aacaccatat attacagttg
     2341 tcagagaaaa ccaggtttaa aaaaagcccg taaagcatca caatcctcaa aacgttaata
     2401 tatacctata tatcagcgat tggaagcact tcatggttca caccatgggg tgttttttgt
     2461 gtaatattca aaaattgtcc taaagtcatt tttaaaactt ctaaatactt gagaaaccat
     2521 tgtaaattta aataactact ttaaata
//
LOCUS       DATROD010000159         2536 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.161, whole genome shotgun sequence.
ACCESSION   DATROD010000159 DATROD010000000
VERSION     DATROD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.161"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            105..1031
                     /locus_tag="VBC74_001956"
     CDS             105..1031
                     /locus_tag="VBC74_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO6942852.1"
                     /translation="MEKIILTATAESIEQVKQLLAIGIDRIYVGEENYGLRLPHSFSD
                     DELREIAKLVHDAGKELTVACNALMHQEMMDNIKPFLELMKEINVDYLVVGDAGVFYI
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVETVLAREIPSEELFKMSENLEFPAE
                     ILVYGASVIHHSKRPLLQNYYNFTHITDEKTRERGLFLAEPGDPESHYSIYEDKHGTH
                     IFINNDINMMTKVTELVEHHFTHWKLDGIYCPGDNFVAIAEIFVETARLIENGTFTQD
                     QAFLFDERIRKLHPKGRGLDTGFYDFDPSTVK"
     gene            1161..2447
                     /locus_tag="VBC74_001957"
     CDS             1161..2447
                     /locus_tag="VBC74_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEO6942853.1"
                     /translation="MSNVKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGINYAHARDAKVYVAANMVTHEGNELGAGPWFRELRDMGLDAVIVSDP
                     ALIVICATEAPGLEIHLSTQASSTNYETFEFWKEMGLTRVVLAREVTMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGQERQSL
                     KGEIPEPFSMSAVDMCMIEHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFEAIKEDLIDELWKVAQRELATGFYYHTPTENEQLFGARRKIPQYKFVGEVV
                     SFDNAKMEATIRQRNVIMEGDRIEFYGPGFRHFECFIEGLRDAEGNKIDRAPNPMELL
                     TITLPNPVKKGDMIRACKEGLVNLYQNDGTSKTIRA"
BASE COUNT          825 a          379 c          528 g          804 t
ORIGIN      
        1 gtgcttaacc tctaaagtga tataattttc gaaaagaata ttttttattt attcttgtca
       61 agaaatatgc ttaaaaatgc tgaaagtttg ttataatact aattatggaa aaaataattt
      121 tgacagcgac agctgaatct attgaacaag taaagcaatt gctagcaata ggaattgacc
      181 gcatctacgt aggggaagaa aattacggtc ttcgtttacc acattcattt tcagacgatg
      241 aattgcgtga aatcgctaaa ttggtacatg atgctggtaa agaattgaca gtagcttgta
      301 atgcactcat gcatcaagaa atgatggata atatcaaacc atttttggag ttgatgaaag
      361 aaattaatgt ggattatttg gtagttggtg atgcaggagt attttatatc aacaagcgtg
      421 atggttataa cttcaaactt atctacgaca cttctgtatt cgttacatca agtcgtcaag
      481 ttaatttttg gggacaacat ggagcagtag aaacggtttt agcacgtgaa ataccttctg
      541 aagaactttt taagatgtct gagaaccttg aatttcctgc tgagatttta gtttatgggg
      601 cttctgtaat tcatcactct aagcgtccgt tattgcaaaa ctattataat tttactcata
      661 tcactgatga aaaaacacgt gagcgtggtc tttttttagc agaaccaggt gatccagaaa
      721 gtcactattc tatttacgaa gataagcatg ggactcatat ttttattaac aatgatatca
      781 atatgatgac taaagtgact gaactagttg aacatcactt tactcactgg aaattagatg
      841 gtatttattg tccaggagat aattttgttg ctattgctga aatttttgtc gaaacagctc
      901 gtttgattga aaatggaaca ttcactcagg atcaggcttt cttgtttgat gaacgaatta
      961 gaaaactgca ccctaaagga cgtggtttag atactggatt ttatgatttt gacccatcaa
     1021 cagtaaaata agatttattt gctacgtgaa agttttttat ctcaaagcgt agccaattgc
     1081 actaagaact gcatatcgat tggtatgtta attaagatag aaaataattc ttttatcttg
     1141 aaaatagaag gaaatataag atgtctaatg taaaaaaacg ccctgaggtt ttatcacctg
     1201 caggaacact agaaaaatta aaagttgcta ttgattatgg agcagatgct gtatttgttg
     1261 gaggtcaagc gtatggtctt cgaagtagag ctggtaactt ttctatggaa gagttacaag
     1321 agggaataaa ctatgctcat gcgagagatg ctaaagttta tgtagcggct aatatggtta
     1381 ctcatgaagg taatgagctt ggggcaggtc cgtggtttcg tgaattacgt gatatgggac
     1441 tagatgcagt cattgtttca gatcctgctc ttattgttat ttgtgctaca gaagcaccag
     1501 gtttagaaat tcatttgtca actcaagcct cttccacgaa ctatgaaact tttgaatttt
     1561 ggaaagagat ggggcttact cgtgtcgtat tagcacgtga ggttactatg gcagagttgg
     1621 ctgaaatcag gaagaggaca gatgttgaga tagaagcatt tgttcatggc gcgatgtgta
     1681 tttcatactc aggacgatgt gttctatcaa accatatgag ccatcgtgat gctaatcgtg
     1741 gcggttgctc tcagtcatgt cgttggaaat atgacctcta cgatatgcca tttggacaag
     1801 aacgtcaatc gttaaaaggc gagattccag aacctttctc aatgtcagct gtggatatgt
     1861 gtatgattga gcatattcca gatatgattg aaaatggcgt agatagttta aaaatagaag
     1921 gacgtatgaa atccattcat tatgtttcta cagtaactaa ttgctataaa gctgctgtag
     1981 atgcctatat ggaaagtcca gaagcttttg aagctattaa agaagacttg attgatgaac
     2041 tttggaaggt tgcacaacgc gaattagcaa caggttttta ctaccataca ccaactgaaa
     2101 acgaacaact ctttggagct cgtcgtaaaa ttcctcaata caaatttgtt ggggaagtgg
     2161 tttcatttga caatgctaaa atggaggcta caattcgtca gcgtaatgtt attatggaag
     2221 gagatcgcat agaattctat ggtcctggct tccgtcactt tgaatgtttt attgaaggtc
     2281 tgcgcgatgc tgaaggaaat aaaatagacc gtgctccaaa tccgatggaa ttattaacca
     2341 taacattacc aaatccagta aaaaaagggg atatgattcg tgcttgtaaa gaaggattag
     2401 tgaaccttta tcaaaatgat ggtactagca agactataag agcttagata cttttataac
     2461 tattttgtca ttttacatgg tcaatattac taaaaattgt taaaatagaa gaagttcatt
     2521 taaatagaac aaaaag
//
LOCUS       DATROD010000160         2514 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.162, whole genome shotgun sequence.
ACCESSION   DATROD010000160 DATROD010000000
VERSION     DATROD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.162"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..752
                     /locus_tag="VBC74_001958"
     CDS             <1..752
                     /locus_tag="VBC74_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEO6942854.1"
                     /translation="NNQYFEIDFKQLEKDIIENNVKLYIFCSPHNPGGRVWTKGEIQK
                     IGDICKRYNVILVSDEIHQDLVLFDNVHHSFNTVDSSFKELSVILSSATKTFNIAGTK
                     NSFAIIENEKLRSDFKKRQIANNQQEISSLGLLATEVAFTKGKQWLKALKMELEGSIE
                     YLYEQLTQKTHIKVMKPEGTYLVWLDFSAYNLTHLEIQEKLRYDAKLILNDGLTFGKE
                     GKKHARINVAAPRSVIEEAVLRLVTNFRKND"
     gene            862..1491
                     /gene="upp"
                     /locus_tag="VBC74_001959"
     CDS             862..1491
                     /gene="upp"
                     /locus_tag="VBC74_001959"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEO6942855.1"
                     /translation="MGKFQVISHPLIQHKLSILRRTTTSTKDFRELVDEIAMLMGYEV
                     SRDLPLEDVEIQTPVATTVQKQLAGKKLAIVPILRAGIGMVDGFLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGAASIKFVCL
                     VAAPEGVAALQEAHPDVDIYTAALDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            1616..2206
                     /locus_tag="VBC74_001960"
     CDS             1616..2206
                     /locus_tag="VBC74_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEO6942856.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGQVEDNMANSIIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKSDVQTIVMGMAASMGTIIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQSDMAIAAEHLLKTRHTLEKILADNSGQ
                     SIEKVHDDAERDRWMSAQETLDYGFIDAIMENNNLQ"
     gene            2306..>2514
                     /locus_tag="VBC74_001961"
     CDS             2306..>2514
                     /locus_tag="VBC74_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2129 domain-containing protein"
                     /protein_id="HEO6942857.1"
                     /translation="MNKQERLGMSVYLYYNRDARKLYKYGDVHYHSRRLRYLVIYVNK
                     EDIVSVSKEIKHLKFVKDVRLSAID"
BASE COUNT          864 a          351 c          501 g          798 t
ORIGIN      
        1 ataataatca gtattttgag attgacttta aacaattaga aaaagatatc attgaaaata
       61 atgtaaagct ttatattttt tgtagtccac acaatcccgg tgggcgtgtc tggacaaaag
      121 gagaaattca aaaaatagga gatatctgta aacgatataa tgttatcctc gtttctgacg
      181 aaatacatca agatttggta ttatttgata atgtacacca tagctttaat actgttgatt
      241 ctagttttaa agaattgagt gttattttat ctagtgcaac taaaacgttt aatattgcag
      301 ggactaaaaa tagttttgca ataattgaaa atgaaaagtt acgctcggat tttaagaaga
      361 ggcaaatagc taacaatcaa caagaaattt cttcattggg gttattagct acagaagttg
      421 cctttacaaa agggaaacaa tggttaaagg ccctaaaaat ggagttagaa ggatcaatag
      481 aataccttta tgagcaatta acgcaaaaaa ctcatatcaa agtgatgaaa ccagagggaa
      541 cttatcttgt ttggttagat ttttctgctt ataatttaac gcatttagaa attcaagaaa
      601 aacttcgata tgatgccaaa ttaatattaa atgatggttt aacttttgga aaagaaggga
      661 aaaaacatgc tagaatcaat gtcgcagctc ctaggtctgt tattgaggag gcagttttgc
      721 gattagtgac aaactttaga aaaaatgact agactataaa aaaagtcgca tggaaagggt
      781 ttttatgtta taatactttg aatgtaaaca agcattagaa tgtttttaat gctcaaaatt
      841 aaatagaaag cggtcattat aatgggaaaa tttcaagtca tttcacatcc acttattcaa
      901 cataaactgt caatcttacg tcgcactaca acatctacta aagattttcg tgagttagtt
      961 gatgagattg cgatgttaat gggctatgaa gtgtctcgtg atttgccttt agaagatgtt
     1021 gaaatccaaa caccagtagc gacaactgta caaaaacaat tagctgggaa aaagttagca
     1081 attgttccaa ttttacgtgc aggtattggt atggttgatg gtttcttaag ccttgtgcca
     1141 gctgctaaag ttggtcatat cggtatgtat cgtgatgaag agactttcca accagtcgaa
     1201 tatttagtta agttacctga agatattgat caacgtcaga tttttgtagt tgatccaatg
     1261 cttgctacag gtgggtctgc tattttagca gtagattctc taaaaaaacg tggcgcagct
     1321 agtatcaaat tcgtttgttt agtagcagct ccagaaggag tggcagcact tcaagaagca
     1381 catccagatg tagatattta taccgctgct ttggatgaaa aactcaatga acatggctac
     1441 attgttcctg gactcggaga tgctggggat cgcttatttg gtacaaaata agatttgaga
     1501 tgaataaggc ttttctgaag aactcagaaa gccttttctt aaaaaagtta ttatttgacc
     1561 tttgttgacc aaatgattat aatagtgaca gtgataaaaa gaaatggaga aagatatgat
     1621 tccagtagtt attgaacaaa caagtcgtgg tgaacgttct tatgatattt actcacgtct
     1681 tttaaaagat cgtattatta tgttgacagg ccaagttgag gataatatgg ccaatagtat
     1741 cattgcacag ttattgtttc tcgatgcaca agataataca aaggatattt acctttatgt
     1801 caatacacca ggtggttcag tatcggctgg acttgctatt gtggacacca tgaacttcat
     1861 taaatcggac gtacagacga ttgttatggg gatggctgct tcgatgggaa ccattattgc
     1921 ttcaagtggt gctaaaggaa aacgttttat gttaccgaat gcagaatata tgatccacca
     1981 accaatgggc ggaacaggcg gaggtacaca gcaatctgat atggctatcg ctgctgagca
     2041 tcttttaaaa acgcgtcata ctttagaaaa aatcttagct gataattctg gtcaatctat
     2101 tgaaaaagtc catgatgatg cagagcgtga tcgttggatg agtgctcaag aaacacttga
     2161 ttatggcttt attgatgcta ttatggaaaa taataattta caataataga tttaaaagag
     2221 ttgagtttac caactctttt tttatttgtt ggaattatgt tataatctta gtaattacag
     2281 atatgacgca gaaaggaaaa aattattgaa taaacaagag cgtttaggaa tgagcgttta
     2341 tctttactat aatcgtgacg ctcgtaaatt atataaatac ggagatgttc attatcattc
     2401 tcgtagatta agatatctag tcatttatgt taataaagag gatattgtta gcgttagtaa
     2461 agagataaaa catttaaaat ttgttaagga tgttcggtta tcagcaattg atga
//
LOCUS       DATROD010000161         2465 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.163, whole genome shotgun sequence.
ACCESSION   DATROD010000161 DATROD010000000
VERSION     DATROD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.163"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            85..225
                     /locus_tag="VBC74_001962"
     CDS             85..225
                     /locus_tag="VBC74_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942858.1"
                     /translation="MLLIDVMIHLSEENEKGFTKLQFFTRQIVVGVNWYVLPKIDNYF
                     NP"
     gene            954..1514
                     /gene="efp"
                     /locus_tag="VBC74_001963"
     CDS             954..1514
                     /gene="efp"
                     /locus_tag="VBC74_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEO6942859.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNNETYDQYEIPTVNIENE
                     LLYILENSEVKIQFYGTEVIGVQIPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            1603..1992
                     /locus_tag="VBC74_001964"
     CDS             1603..1992
                     /locus_tag="VBC74_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEO6942860.1"
                     /translation="MTTENLGEIVISPRVLEVITGIAATKVDGVHSLRNKAVTDSLSK
                     KSLGRGVYLKNEEDDTVAADIYVYLQYGVNVPAVSIAIQQAVKTAVYDMAEVKISSVN
                     IHVEGIVPEKTPKPDLKSLFDEDFLDD"
     gene            1985..2419
                     /gene="nusB"
                     /locus_tag="VBC74_001965"
     CDS             1985..2419
                     /gene="nusB"
                     /locus_tag="VBC74_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEO6942861.1"
                     /translation="MTSVFKDSRRDLRERAFQTLFSLETGGEFIDAAHFAYGYDKTVS
                     EDKVLEVPIFLLNLVNGVVDHKDELDTLISSHLKSGWSLERLTLVDKSLLRLGLYEIK
                     YFDETPDRVALNEIIEIAKKYSDETSAKFVNGLLSQFITNEN"
BASE COUNT          892 a          396 c          427 g          750 t
ORIGIN      
        1 caaagggaaa gactagtgta gaatagaaat aatctaaaaa gtaaattcta ctcaaaaaca
       61 aatcaatatg ctactagtgt gaatatgcta ttgatagatg tcatgataca cttatcagaa
      121 gaaaacgaaa aaggctttac taaattacaa ttctttactc gacaaattgt ggttggtgta
      181 aactggtatg tacttcccaa aattgataac tatttcaacc cctaaaagat aagaaaaaat
      241 acttttgaaa atagactgta ctttaggaaa atcccgtata aaagaatagc taataaagcc
      301 agcgcaggta tctgtacgat tagaaaacga catagttgta actttctata atgagacata
      361 ttaaaattca tcattccacc tcctaaatta gtaattcaat gatcagtgga ctagggctcc
      421 ttcaatacca atgacagctc ttattccaat tgcatatggc actaaaacag agagctacct
      481 gatgtagtaa gcaagattgg tatcaccaag ctcagtacca atgcttagcg cacgtccact
      541 aacaccaccc cgatttcctt taccagcttc aatagagcaa attaagttcg tatctaaaac
      601 ttatatcgag aataactaca ttttcatacg aatagctccg taggtgattt attttatctg
      661 tttcaataag acaactatag tttactacct ttattaaaat ttactaaaaa gtcactaaaa
      721 acacctaaac agtatttcaa tcttagtcaa gatatcggta taatattaaa actgttatca
      781 aaactgaagt aagaggaaag tgtcaagtgc ttcagtctta tttacgccct cttggtgatt
      841 ttatttattt atcaagaaat cagacacata ccaaatcaat aaactatttg agaaagcagg
      901 ttatttgtga taaaatgagt tgataaataa tttttacaaa gaggtattaa taaatgattg
      961 aagcaagtaa gcttaaagca ggcatgactt ttgaaacggc tgatgggaaa ttaatccgtg
     1021 ttcttgaagc tagccaccac aaaccaggta aaggtaacac aatcatgcgt atgaaattgc
     1081 gtgatgttcg tactggttca acttttgata caagttaccg tcctgaagaa aaatttgagc
     1141 aagctattat tgaaactgta cctgctcaat acctttataa gatggatgat acagcttact
     1201 tcatgaataa tgaaacttac gatcaatatg aaattccaac tgttaacatt gaaaatgaat
     1261 tactctacat tcttgaaaac tcagaagtaa aaattcaatt ctatggaaca gaagttattg
     1321 gtgttcaaat tccaacaact gttgaattga cagttgctga aactcaacca tcaatcaaag
     1381 gtgctactgt aactggttca ggaaaacctg caactatgga aacaggactt gttgttaacg
     1441 ttccagactt tattgaagct ggacaaaagt tagtaatcaa tacagcagaa ggaacttacg
     1501 tttcacgcgc ctaatgactc tcaaaaaagt gttaagtaga ttcatgatga atttactcta
     1561 ctaaggaaaa accttagtaa acactaaaag aaaggataag ctatgacaac tgaaaatctt
     1621 ggagaaattg tcatttcacc acgtgtactt gaggtaatta caggtattgc tgcaactaaa
     1681 gttgatggtg ttcattcgtt acgtaacaaa gcggtaacgg atagccttag taagaaatct
     1741 ctaggtagag gtgtctacct taaaaatgaa gaagatgata cagtagctgc agatatttat
     1801 gtttacttgc agtatggtgt aaatgtacca gcagtttcaa ttgctattca acaagctgtc
     1861 aaaacagctg tatatgacat ggcagaagta aaaatttctt cggtcaatat ccatgttgaa
     1921 ggtattgttc cagagaaaac accaaaacca gacttgaagt cattgtttga tgaggatttc
     1981 ttagatgact agtgtattta aagattcaag acgtgattta cgtgaacgtg cttttcaaac
     2041 actatttagc ttagaaactg gtggagagtt tattgatgcg gctcactttg cttatggtta
     2101 tgataaaaca gtatctgaag acaaagtttt agaggttcca atctttttac ttaatcttgt
     2161 taatggtgtt gtagatcaca aggatgaatt agatactttg atttctagtc acttaaaatc
     2221 aggatggagc ttagaacgtc taacattggt tgataaatct ctcttacgct taggcctcta
     2281 tgaaattaaa tattttgatg aaacgcctga tcgtgtagct ctaaatgaga ttatcgagat
     2341 tgctaaaaaa tattcagatg agacgtcagc aaaatttgtc aatggtttat taagccaatt
     2401 tataacaaat gaaaattaaa aatgtggaag aagattcttc cacattttta atttgctttg
     2461 aaaaa
//
LOCUS       DATROD010000162         2455 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.164, whole genome shotgun sequence.
ACCESSION   DATROD010000162 DATROD010000000
VERSION     DATROD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.164"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..372
                     /locus_tag="VBC74_001966"
     CDS             <1..372
                     /locus_tag="VBC74_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6942862.1"
                     /translation="KVDMNTILGQKTVMIHINVAKKDMNSTSMMMKKDKMTMPMKKEM
                     TSSKINTGMMMSNNKMSANMQMSSQAKSNDKAGKKMSMMSKNLPNTGETKQQNVGVLG
                     MLSLAFATGLTALGLKKSKQR"
     gene            485..1171
                     /locus_tag="VBC74_001967"
     CDS             485..1171
                     /locus_tag="VBC74_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEO6942863.1"
                     /translation="MTQKLLLVDDEFEIIDINRRYLEQAGYEVSVAADGIEALKEVDE
                     NRFDLIISDIMMPKMDGYDFISEVLVREPNQPFLFITAKVSEPDKIYSLSMGADDFIS
                     KPFSPRELVLRVKNILRRIYGNHQQSEVLTIGDLVIDQKQRLVMVDCNTISLTNKSFD
                     LLWILANHLNRVFSKTELYERVWGEEFLDDTNTLNVHIHALRNDLAKFSTDNTPTIKT
                     VWGLGYKLEE"
     gene            1173..2210
                     /locus_tag="VBC74_001968"
     CDS             1173..2210
                     /locus_tag="VBC74_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEO6942864.1"
                     /translation="MKLKYYIVIGYLISMLITVAGVFFGLNHMLIETRGVYYILSVTI
                     IACIVGGIVNLFLLSSVFTSLKKLKQKMKDISQRRFDTKAQICSPQEFKDLETAFNQM
                     SSELESTFKSLNESEREKTMMIAQLSHDIKTPITSIQSTVEGILDGIISEEEVNYYLN
                     TISRQTNRLNHLVEELSFITLETMSDTAEPHKEETIYLDKLLIDILSEFQLVFEKENR
                     QVMIDVAPDVSKLSSQYDKLSRILLNLISNAVKYSDPGSPLTIKAYSNRQDIVIDIID
                     QGYGIKDEDLASIFNRFYRVESSRNMKTGGHGLGLYIARQLAHQLNGDILVESQYQKG
                     SKFSLVLKLQK"
     gene            complement(2224..>2455)
                     /locus_tag="VBC74_001969"
     CDS             complement(2224..>2455)
                     /locus_tag="VBC74_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEO6942865.1"
                     /translation="VFYTQLESGNYEGPSKVLVASRELMNGNKLRLFLLDLSFIGWHF
                     LTIFSFGLVYIYLLPYQTTARLIFYRNITKNS"
BASE COUNT          871 a          360 c          442 g          782 t
ORIGIN      
        1 aaagttgata tgaatactat tttaggacaa aagacagtga tgattcatat taatgtcgct
       61 aaaaaagata tgaatagcac atcaatgatg atgaaaaaag ataagatgac aatgcctatg
      121 aagaaagaaa tgacttcatc taaaataaat accggtatga tgatgtctaa caacaagatg
      181 tcagctaata tgcaaatgtc atcacaagcc aaatcaaatg ataaagctgg taaaaaaatg
      241 tctatgatgt ctaaaaattt acctaataca ggtgaaacaa agcaacaaaa tgtaggtgtg
      301 ctaggtatgc ttagtctagc ctttgcgaca ggtctaactg cgctaggcct taaaaaatct
      361 aaacaaagat agggatagta tttaattcag aacaccaaat ttgtttaaaa ttattgaggg
      421 ttatcgtggt aaaatagtgt tatactagag gtatagcact attttgactc attaggagag
      481 tagaatgaca caaaaattat tattagttga cgatgaattt gaaattattg atattaatag
      541 acgttatcta gagcaagcag gatatgaagt gtcagtagct gcggatggca tagaagcact
      601 caaagaggta gatgagaatc gttttgacct aattattagt gatattatga tgcctaaaat
      661 ggatggctat gattttatta gtgaagttct agttcgagaa cccaaccaac cttttctctt
      721 tataaccgct aaagtatctg aaccagataa aatttactct ttgagtatgg gtgcagatga
      781 ctttatttcc aagcctttca gtcctaggga attagtttta cgtgttaaaa atattctaag
      841 acgtatctat gggaatcatc aacaatctga agtattgacg attggtgatt tggttatcga
      901 ccaaaaacaa cgtttggtaa tggtagactg caatacaatt tccttaacca ataaatcatt
      961 tgacttgctt tggatattag caaatcattt gaatcgtgtt ttttcaaaaa cagagcttta
     1021 tgaacgtgtc tggggcgaag aatttttaga tgacactaat actttgaatg ttcatatcca
     1081 tgccttacgc aatgaccttg ctaaatttag tacagacaat accccaacta tcaaaacagt
     1141 ttggggatta gggtataaat tagaggaata aaatgaaatt aaaatattat attgttattg
     1201 gttaccttat ttctatgtta ataactgtag caggtgtttt ctttgggctt aatcatatgc
     1261 tgattgagac tcgaggtgtt tactacatcc tttcagttac aattattgcc tgtatcgtag
     1321 gtggtatagt taatttgttc ttattatcaa gtgtatttac atctttgaaa aagttaaaac
     1381 aaaaaatgaa ggatatttct caaagacgtt ttgatacaaa agcacaaatt tgttcaccac
     1441 aagaattcaa ggatttagag acagccttta accaaatgtc ttcagagtta gagtcaacgt
     1501 ttaaatctct aaacgagagt gaacgcgaaa aaacaatgat gattgcacaa ctttcacatg
     1561 atatcaagac tccaattacc tctatacaat caacagtaga aggtatttta gatggtatta
     1621 tatctgaaga agaagttaac tattatctca ataccatttc tcgtcaaaca aaccgtctta
     1681 accatctagt tgaagaatta agttttatta cattagagac tatgtcagac acggctgaac
     1741 ctcataaaga agaaacaatt tatctggata aactactgat tgatatttta tctgaatttc
     1801 aattggtgtt tgaaaaagaa aatcggcaag taatgattga tgttgcgcca gatgtttcga
     1861 aattatctag tcaatatgat aaattatcac gaattttatt gaatttgatt agcaatgcag
     1921 tcaaatattc tgatcctgga agtcccttga ctatcaaggc ctattcaaat cgtcaagata
     1981 tcgttatcga tattattgac caaggctatg ggataaaaga tgaagatctt gcttctattt
     2041 ttaatcgttt ttatcgtgta gaatcttcac gaaatatgaa gacaggagga catggcctag
     2101 gtctttatat tgcgcgacaa cttgcacatc aactgaatgg tgatattttg gtcgaaagtc
     2161 agtatcaaaa aggcagtaag ttctctttag tacttaaatt acaaaaatag ctaggaatta
     2221 ttcctagcta ttttttgtaa tgtttctata aaagattaag cgcgcagtag tttggtaagg
     2281 aaggagatag atatagacta atccgaaact gaagattgta aggaaatgcc aaccaataaa
     2341 acttaagtct aaaaggaaga gtctaagttt attaccattc attagttctc gactagcaac
     2401 tagtacttta gatggtccct catagtttcc actttctaat tgagtgtaaa atact
//
LOCUS       DATROD010000163         2370 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.165, whole genome shotgun sequence.
ACCESSION   DATROD010000163 DATROD010000000
VERSION     DATROD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.165"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            414..623
                     /locus_tag="VBC74_001970"
     CDS             414..623
                     /locus_tag="VBC74_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000965644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEO6942866.1"
                     /translation="MLYIDEFKEAIDKGYISGDTVAIVRKNGKIFDYVLPHEKVRDDE
                     VVAVESVEDVLAELGNVRCDISSCQ"
     gene            999..1196
                     /locus_tag="VBC74_001971"
     CDS             999..1196
                     /locus_tag="VBC74_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942867.1"
                     /translation="MSEKAILLIILIAFVIIAIIKIALLKLYMNTIKPKVDNEGNIIT
                     KPVKDDLTPEEREEREFDKDW"
     gene            complement(1240..2172)
                     /locus_tag="VBC74_001972"
     CDS             complement(1240..2172)
                     /locus_tag="VBC74_001972"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEO6942868.1"
                     /translation="MVSLKTEIAGFSFDNCLMNAAGIYCMTKEELLAIENSEAGSFVT
                     KTGTLKAREGNPQPRYADTDWGSINSMGLPNKGIDYYLDFVTELQDQDNSKNHVLSLV
                     GLSPEETHIILKKVENSSYNGLIELNLSCPNVPGKPQIAYDFEMTDLILSEIFSYYQK
                     PLGIKLPPYFDIVHFDQAATIFNKYPLAFINCVNSIGNGLVIDDETVVIKPKNGFGGI
                     GGDFIKPTALANVHAFYKRLNPSIKIIGTGGVKNGRDAFEHILCGASMVQIGTALQKE
                     GPEIFQRVSRELKEIMADKGYQSLEDFRGQLNYL"
BASE COUNT          829 a          366 c          420 g          755 t
ORIGIN      
        1 gatttaacta aaaatacaat gaaattgagt gttgtttttt ataaagacaa caagaagaaa
       61 ttagttgttg taggattaaa aagagatgag acaggggtct ttaggccagc tacgttgcat
      121 gagagccgaa acaaccccta caagcgaatt cggcatactg ctataaaatc aataacttgg
      181 atttagaaca taccttttgt gctgattacc tctattgaca cactcgcata atcatgagat
      241 aatcacagta gcaagaatcg cctgaaagta gcagtagatg ctgtcaaaga aaaactgaaa
      301 taaatattaa ccgctcattt aatgagcggt tattttttgt ctatcagaac agaaaaattt
      361 aaaattgtct atttttagga ttttttatcg aatagataag tggaggataa aatatgttat
      421 atatagatga gtttaaagaa gcgattgata aaggctatat ttcaggggac acagtagcga
      481 tagtgcgtaa aaacggaaag atatttgatt atgtgttacc acacgaaaaa gtgagagatg
      541 atgaagttgt ggcagtagag agtgtggaag acgtactcgc agaactgggt aatgttcgtt
      601 gtgatataag tagctgtcaa tagtttgccc caactaacaa tatacttttt tgtggggcga
      661 atttggggca taagtttaaa actaatctat gttaacaatg atacgacttt tatgtttact
      721 actcataacg acttatatag atatgtttta gaccttataa tgccaaccga aaaacctaac
      781 tcttaaagac gctgttaaat aattcgtcta gaaaaacctt gtcatatcaa tgtttattga
      841 tagcgacaag gttctttttt atatccaata ctcaataaac gactagagag ctcaaaggaa
      901 tttagagttt caaatgtgtt agagcatacc cttgaattat cttaactgtg atgttataat
      961 tcctaaaaga taagttaaaa gagaggttgc gaaagagaat gtcagaaaaa gctatactat
     1021 taattatcct tattgctttt gttattatcg ctattataaa gatagctctg ttaaagttat
     1081 atatgaatac gataaaacct aaagtagata atgaaggaaa catcataaca aagcctgtaa
     1141 aggatgattt aacacccgag gaacgtgaag aacgtgaatt tgataaagat tggtaaaatc
     1201 aatataaaag agcaaacaag gtttgctctt ttattatttt taaaggtaat taagttgtcc
     1261 tcgaaaatcc tccaatgatt gatagccttt atcagccatt atttctttta actctctgct
     1321 tacacgctga aaaatttctg gtccttcttt ttgtaaagcg gttccgattt ggaccatact
     1381 agctccacat agaatgtgtt caaaggcatc acggccgttt ttaacaccac ctgttccgat
     1441 aattttaatt gaaggattta aacgtttgta gaaagcatgg acgttagcta aggcggtagg
     1501 cttgataaaa tcaccgccga tacctccaaa gccattttta ggctttataa cgacagtttc
     1561 atcatcaata acaagtccat ttccgataga attaacacag ttaataaaag ctaaaggata
     1621 tttgttaaaa attgtagctg cttgatcaaa gtgaacgata tcaaagtaag gtggtaattt
     1681 gatgcccaat ggcttttgat aatagctaaa gatttctgaa aggatgaggt cagtcatttc
     1741 aaaatcataa gcaatttgag gttttccagg aacatttggg cacgataaat ttaactctat
     1801 caggccattg taactactgt tttcaacctt tttaagaata atatgtgtct cttctggtga
     1861 taaacctact aatgataaaa cgtgattttt gctattgtct tggtcttgta attcagtcac
     1921 gaaatcaaga taatagtcaa tacctttatt agggagtccc atagaattaa ttgatcccca
     1981 gtcagtatct gcataacgtg gttgagggtt tccctctcta gctttaaggg tacctgtttt
     2041 tgtaacaaag gaaccagctt cagaattttc aatagctaat aactcttcct tagtcataca
     2101 ataaataccc gctgcattca tcaaacaatt gtcaaaacta aaaccagcaa tttccgtttt
     2161 tagcgatacc ataatatcct cctgaagata aatttttact attttatcac ttgattaaca
     2221 gagcggcaag aaaataatga tattggtaat aaaatttcgc taaataacga acgttattat
     2281 ttgtgtaaac aataatagta aagaaataaa tgttgctttc tataacgcat aaaaaaataa
     2341 gcttatgtaa gcttattttt tatgttcggc
//
LOCUS       DATROD010000164         2220 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.166, whole genome shotgun sequence.
ACCESSION   DATROD010000164 DATROD010000000
VERSION     DATROD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.166"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(21..797)
                     /locus_tag="VBC74_001973"
     CDS             complement(21..797)
                     /locus_tag="VBC74_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEO6942869.1"
                     /translation="MLKRERLQKIIEKVNINGIVTVNEIMEELDVSDMTVRRDLDELD
                     KAGLLIRIHGGAQKVNAAPIPQNYEKSNTEKYDIQTNEKLEIAQFAKQFINDGETIFI
                     GPGTTLEKLATQLLDFKIRVVTNSLPVFNILNQSSTLDLILVGGEYREITGAFVGSVT
                     INSIKSLNFSKAFVSSNGVFEKSIATYDEGEGEIQRIALNNSFEKFLLVDSQKFGKYD
                     FYTFYQLDDIDFVLTDHNIDNVVKEQYSSFTKILTNNNYM"
     gene            1007..1480
                     /locus_tag="VBC74_001974"
     CDS             1007..1480
                     /locus_tag="VBC74_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEO6942870.1"
                     /translation="MFAVTQDILFIDAHSQEELFDLVSKALIKQHYVSPDYRQAVKER
                     EREFPTGLKIDLKDGTPIQYVAIPHTETQYCLVDRIFYVKNSQPITFKHMINPEEECR
                     VQDFFFIINSRNSNQSDILSNLITFFITKGNLDRLHELGDNKEKINHYLIEKGVF"
     gene            1482..1787
                     /locus_tag="VBC74_001975"
     CDS             1482..1787
                     /locus_tag="VBC74_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEO6942871.1"
                     /translation="MIKILAACGAGINSSHQIKDAIETQLGDRGYNVHCDAVMVKDIT
                     EEMVNKYDIFTPIAKTDLGFNVPIPVVEAGPILYRIPVMSEPVFTALEQVIKEHNLS"
     gene            1827..2204
                     /locus_tag="VBC74_001976"
     CDS             1827..2204
                     /locus_tag="VBC74_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HEO6942872.1"
                     /translation="MQVFLNIVNKFFDPVIHMGSGVVMLIVMTGLAMIFGVKFSKALE
                     GGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGINLSIIDVGWAPLATITWGSPYT
                     LYFLLIMLIVNIVMIVMKKKRIP"
BASE COUNT          744 a          332 c          369 g          775 t
ORIGIN      
        1 gtagtggctc acttgtttta ttacatgtaa ttgttattag ttaaaatttt ggtgaagcta
       61 ctgtattgct ccttaacaac attatcaata ttatgatctg ttaatacaaa atcaatatcg
      121 tctaattgat aaaaagtata aaaatcatat ttaccaaatt tttggctatc aactaaaagg
      181 aatttttcaa aggaattatt aagagcaatg cgctggattt caccttctcc ctcgtcataa
      241 gtagcgatag acttttcaaa aacaccatta ctactaacaa aagcttttga aaaattaagt
      301 gatttaatag agttaatagt aacagaaccg acaaaagcac cagtaatttc tctgtactct
      361 cctcctacaa ggataaggtc aagtgttgaa ctttgattca gaatattgaa aacaggaagg
      421 ctattggtta ctaccctaat tttaaaatca agtaattgcg tagctaattt ctctaaggtt
      481 gttccaggcc caataaagat tgtttcacca tcattgatga attgttttgc aaattgggca
      541 atttctaatt tttcatttgt ttgaatatcg tatttttcag tgtttgattt ttcataattt
      601 tgtggaattg gtgctgcgtt tactttttgt gcaccaccat ggatgcgtat taacagtcct
      661 gctttatcta attcatccaa gtcacgccta acagtcatgt ctgatacatc aagttcttcc
      721 atgatttcat taacagtgac aataccattt atattgactt tttctattat tttttgtaat
      781 ctttctcgct ttaacataat aatctctcct acctcttcat tgtatatatc ataccataaa
      841 tagagctaaa ataataataa ttttgaaaaa aacaaacaat aataaacaaa aataaataaa
      901 aaacgtcttg tgatgtttta ttttgttttg tataatattg atgtagataa gttgataagg
      961 cttgtttgcc ttacaacaaa atgtaaccgc ttaactaaaa agaaaggtgt ttgctgtgac
     1021 tcaagatatt ttatttattg atgcccattc tcaggaagaa ttatttgact tagtatcaaa
     1081 agcattgata aaacaacact atgttagtcc tgactaccga caggcagtaa aagaaaggga
     1141 gagagagttt ccaacggggt taaaaattga tttaaaagat gggactccta ttcaatatgt
     1201 tgctattcca cacactgaaa cgcagtattg tctagtagat agaatctttt atgtcaaaaa
     1261 tagtcagcca attactttca aacatatgat taatccagaa gaggagtgta gggttcagga
     1321 ttttttcttc atcattaata gtaggaatag taaccaaagt gatattttat ctaatttgat
     1381 taccttcttt attacaaaag gaaacctaga tcggctacat gagcttggcg acaataagga
     1441 aaaaattaat cattatttaa tagaaaaagg agtgttttaa catgatcaaa atattagcag
     1501 cttgtggtgc aggcataaat tcaagtcatc aaattaaaga tgctatcgag acacaattag
     1561 gagatagagg ttacaatgta cattgtgatg ctgttatggt taaggatatc actgaggaaa
     1621 tggtcaacaa atatgatatc tttactccta ttgctaaaac tgatttaggt tttaatgtac
     1681 caattccagt tgtagaagca ggacctattc tttatcgcat accagttatg agcgaaccgg
     1741 tctttactgc tttagaacaa gttatcaaag aacacaacct atcataacat atttaaaatt
     1801 ggaattagtc ttaaaggaga aggaacatgc aggtattttt aaatattgtc aataaattct
     1861 ttgatccagt tattcatatg ggttcgggag ttgtgatgct aattgtcatg acaggtttag
     1921 ccatgatatt tggagtgaag ttttctaaag cacttgaagg tggtattaag ttagctattg
     1981 ctcttacggg tattggtgct attattggta ttttaactgg tgctttttcc gaatcacttc
     2041 aagcttttgt taaaaataca ggaatcaatc taagcattat tgacgttggt tgggctccat
     2101 tagcaactat tacatgggga tcaccatata cgctttactt cttattaatc atgcttattg
     2161 tcaatattgt tatgattgtt atgaaaaaaa aacggatacc ttagatgttg atatttttga
//
LOCUS       DATROD010000165         2191 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.167, whole genome shotgun sequence.
ACCESSION   DATROD010000165 DATROD010000000
VERSION     DATROD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.167"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            87..2126
                     /locus_tag="VBC74_001977"
     CDS             87..2126
                     /locus_tag="VBC74_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6942873.1"
                     /translation="MKRKYFVLNTVTVLTLATAMNTSSIYANSTETSASVAPTTNTIV
                     QTNDSNPTAKFASESGQSVIGQVKPANSAALTTVDTPHISAPDALKTTQSSPVVESPS
                     TKLTEETYKQKDGQDLANMVRSGQVTSEELVNMAYDIIAKENPSLNAVITTRRQEAIE
                     EARKLKDTNQPFLGVPLLVKGLGHSIKGGETNNGLIYADGKISTFDSSYVKKYKDLGF
                     IILGQTNFPEYGWRNITDSKLYGPTHNPWNLAHNAGGSSGGSAAAIASGMTPIASGSD
                     AGGSIRIPSSWTGLVGLKPTRGLVSNEKPDSYSTAVHFPLTKSSRDAETLLTYLKKSD
                     QTLVSVNDLKSLPIAYTLKSPMGTEVSQDAKNAIMDNVTFLRKQGFKVTEIDLPIDGR
                     ALMRDYSTLAIGMGGAFSTIEKDLKKHGFTKEDVDPITWAVHVIYQNSDKAELKKSIV
                     EAQKHMDDYRKAMEKLHKQFPIFLSPTTASLAPLNTDPYVTEKDKRAIYNMENLSQEE
                     RIALFNRQWEPMLRRTPFTPIANMTGLPAISIPTYLSESGLPIGMMLMAGANYDMVLI
                     KFATFFEKHHGFNVKWQRIIDKEVKPSADLIQPTNSLFKAHSSLVNLEENSQVTQVSI
                     SKKWMKSSVKNKPSVMAYQKALPKTGDTESSLSPVLVVTLLLACFSFVTKKNQKS"
BASE COUNT          767 a          375 c          400 g          649 t
ORIGIN      
        1 aatataaaat aacagttaat attttagcaa tttgataaca cttagattat aatgtaaacg
       61 tatacttttt tagattggaa gatgaaatga aacgtaaata ctttgttctt aatacggtga
      121 cggttttaac gttagctact gcaatgaata ctagcagtat ctatgctaat agtactgaga
      181 caagtgcttc agtagctcct actacaaata ctatcgttca aactaatgac agtaatccta
      241 ccgcaaaatt tgcatcagaa tcaggacaat ctgtaatagg tcaagtaaaa ccagctaatt
      301 ctgcggcgct tacaacagtt gacacgcctc atatttcagc tccagatgct ttaaaaacaa
      361 ctcaatcaag tcctgtcgtt gagagtcctt ctactaagtt aactgaagag acatacaaac
      421 aaaaagatgg tcaagattta gccaacatgg tgagaagtgg tcaagttact agtgaggaac
      481 tcgtcaatat ggcatacgat attatcgcta aagaaaaccc atctttaaat gcagtcatta
      541 ctactagacg ccaagaagcc attgaagagg ctagaaaact taaagatact aatcagccgt
      601 ttttaggtgt tcccttgtta gtcaaggggt tagggcacag tattaaaggt ggtgaaacca
      661 ataatggctt gatctatgca gatggaaaaa ttagcacatt tgacagtagc tatgtcaaaa
      721 aatataaaga tttaggattt attattttag gacaaacgaa ttttccagag tatgggtggc
      781 gtaatataac agactctaaa ttatacggtc caacgcataa tccttggaat cttgctcata
      841 acgctggtgg ctcttctggt ggaagtgcag cagctattgc tagcggaatg acgccaattg
      901 ctagcggcag tgatgctggt ggttctatcc gtattccatc ttcttggacg ggcttagtag
      961 gtttaaaacc aacaagagga ttggtgagta atgaaaagcc agattcgtat agtacagcag
     1021 ttcattttcc attaactaag tcatctagag acgcagaaac attgttaact tacctaaaga
     1081 aaagcgatca aacgctagta tcagttaatg atttaaaatc tttaccaatt gcttatactt
     1141 tgaaatcacc aatgggaaca gaagttagtc aagatgctaa aaatgctatt atggacaacg
     1201 tcacattctt aagaaaacaa ggattcaaag tgacagagat agatttacca attgatggta
     1261 gagcattaat gcgtgattat tcaaccttgg ctattggcat gggaggagct ttttcaacaa
     1321 ttgaaaaaga cttaaaaaaa catggtttta ctaaagaaga cgttgatccc attacttggg
     1381 cagttcatgt tatttatcaa aattcagata aggctgaact taagaaatct attgtggaag
     1441 cccaaaaaca tatggatgat tatcgtaagg caatggagaa gcttcacaag caatttccta
     1501 ttttcttatc gccaacgacc gcaagtttag cccctctaaa tacagatcca tatgtaacag
     1561 agaaagataa aagagcgatt tataatatgg aaaacttgag ccaagaagaa agaattgctc
     1621 tctttaatcg ccagtgggag cctatgttgc gtagaacacc ttttacacca attgctaata
     1681 tgacaggact cccagctatc agtatcccga cttacttatc tgagtctggt ttacccatag
     1741 ggatgatgtt aatggcaggt gcaaactatg atatggtatt aattaaattt gcaactttct
     1801 ttgaaaaaca tcatggtttt aatgttaaat ggcaaagaat aatagataaa gaagtgaaac
     1861 catctgctga cctaatacag cctactaact ccctctttaa agctcattca tcattagtaa
     1921 atttagaaga aaattcacaa gttactcaag tatctatctc taaaaaatgg atgaaatcgt
     1981 ctgttaaaaa taaaccatcc gtaatggcat atcaaaaagc acttcctaaa acaggtgata
     2041 cagaatcaag tctatctcca gttttagtag taaccctttt attagcttgt tttagctttg
     2101 taacaaaaaa gaatcagaaa agttgattgt aatgtaaaat aattcctatt tttcactttt
     2161 tagtatcata ttttcgattt ttatgttgta a
//
LOCUS       DATROD010000166         2173 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.168, whole genome shotgun sequence.
ACCESSION   DATROD010000166 DATROD010000000
VERSION     DATROD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.168"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            118..1347
                     /locus_tag="VBC74_001978"
     CDS             118..1347
                     /locus_tag="VBC74_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEO6942874.1"
                     /translation="MKKKLVSSLLKCSLIIIVSFAGGAFASFVMNHNDNIPNGGVTKT
                     SKVNYNNITPTTKAVKKVQNSVVSVINYKQQESRSDLSDFYSHFFGNQGGNTDKGLQV
                     YGEGSGVIYKKDGKNAYVVTNNHVIDGAKQIEIQLADGSKAVGKLVGSDTYSDLAVVK
                     IPSDKVSNIAEFADSSKLNIGETAIAIGSPLGTEYANSVTQGIVSSLKRTVTMTNEEG
                     QTVSTNAIQTDAAINPGNSGGALINIEGQVIGINSSKISSTSNQTSGQSSGNSVEGMG
                     FAIPSNDVVKIINQLESNGQVERPALGISMAGLSNLPSDVISKLKIPSNVTNGIVVAS
                     IQSGMPAQGKLKKYDVITKVDDKEVASPSDLQSLLYGHQVGDSITVTFYRGENKQTVT
                     IKLTKTSKDLAKQRANN"
     gene            1445..>2173
                     /locus_tag="VBC74_001979"
     CDS             1445..>2173
                     /locus_tag="VBC74_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEO6942875.1"
                     /translation="MEYLETININHIAPNPYQPRLEFNTKELEELADSIKINGLIQPI
                     IVRPSAVFGYELVAGERRLRAAKLAKLESIPAIIKSYNNDDSMQLAIVENLQRSNLSP
                     IEEAKAYSQLLQKKSMTHEELAKYMGKSRPYISNTIRLLNLPPLITSAIEEGKLSSGH
                     ARALLSLPDASQQKDWYQRILTEDISVRRLEKLLKQEKKTNHKSLQNKDVFLKHQENE
                     LAQFLGSKVKLTINKDGAGNIKIAF"
BASE COUNT          790 a          364 c          385 g          634 t
ORIGIN      
        1 ctataactat aatcaaggtt atccacaact tgtggatatt tccttttttt agcttttaag
       61 gtataattaa gaagcttgtc ataagattag tataaaaatt tacggaggta gctatttgtg
      121 aaaaaaaaat tagtctcatc acttctaaag tgttctctaa tcattattgt tagctttgct
      181 ggtggagcat ttgctagttt tgtcatgaat cataatgaca atattccaaa tggtggtgtc
      241 actaaaacta gtaaagtaaa ttataataac ataacgccta caacaaaagc tgttaaaaag
      301 gtacaaaata gtgttgtttc tgttatcaat tataaacaac aagagagtcg ttctgaccta
      361 tcagacttct atagtcattt ttttggtaat caggggggca acactgataa gggcttacaa
      421 gtttacggtg aaggctctgg agtcatctat aaaaaagatg gtaaaaatgc ctatgttgtc
      481 actaataacc acgtcattga tggggctaaa caaattgaaa ttcaactagc tgatggctca
      541 aaagcagttg ggaaacttgt tgggtcagat acctactctg atttagccgt cgtcaaaatt
      601 ccatcagata aagtttcaaa tattgcagaa tttgctgatt catcaaaact caacattggt
      661 gaaactgcta tagcgatcgg aagccctctt ggaactgagt atgcaaattc tgtaactcaa
      721 ggtattgtat ctagtttaaa aagaactgta acaatgacta atgaagaagg acaaacagtt
      781 tctacaaatg ctatccagac ggatgctgct atcaatcctg gtaattcagg tggagcactt
      841 atcaatattg aaggacaggt tattggaatt aattctagta aaatttcttc tacatcaaat
      901 caaacctcag gacaatcgtc aggaaatagc gttgaaggta tgggatttgc cattccttca
      961 aatgatgttg ttaagattat caatcaactt gagagtaacg gacaagtaga gagacctgct
     1021 ctaggtattt ctatggctgg attaagtaat ttaccatccg atgttattag taaactgaaa
     1081 atcccaagta atgttactaa tggtattgta gtagcatcta tccaatctgg catgccagct
     1141 caaggcaaac taaagaaata cgatgtcatt actaaagttg acgataaaga agtagcatct
     1201 ccaagtgatt tacaaagttt actctatggc caccaggtag gggattccat aacagtaacc
     1261 ttttatcgtg gtgaaaataa acaaacagtc actataaaac ttactaaaac tagtaaagat
     1321 ttagctaaac aacgagcaaa taactaaatc aattctagtt attttaacta agatatagta
     1381 aaattaaagg ggttggtcag atagaccaat ccctttagta agtctataga atctgaggtc
     1441 taaaatggaa tatttagaaa cgatcaacat caatcatatt gctccaaatc cgtatcaacc
     1501 tcgcctagaa tttaatacaa aagaactaga agaattagcc gactctatta aaattaatgg
     1561 ccttattcaa cccattattg taagaccgtc agcagtcttt ggttacgaac tggtagccgg
     1621 agaacgacgg ttaagagccg caaagttagc taaattggaa agtatacctg caattataaa
     1681 atcctataat aatgatgata gtatgcaatt agctattgtc gaaaatttac aacgatctaa
     1741 tttgtcaccc attgaagagg ctaaagccta ctctcaactt ctacaaaaga aaagtatgac
     1801 acatgaagag ttagcaaaat atatgggaaa atctcgtccc tatatatcaa atacgatacg
     1861 actactaaat ttaccgccac taattacttc agctattgaa gaaggaaaac tttcctctgg
     1921 tcatgcgcgt gcactactca gtctaccaga tgctagtcaa caaaaagatt ggtatcagag
     1981 aatcctcact gaagatatta gtgtaagacg actagagaag ttattaaaac aagaaaagaa
     2041 aacaaatcat aagtcacttc aaaataaaga tgtctttcta aaacatcaag aaaatgagtt
     2101 agctcaattt ctaggtagca aagtgaaact tactattaat aaagatggtg ctggcaatat
     2161 aaagattgct ttc
//
LOCUS       DATROD010000167         2155 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.169, whole genome shotgun sequence.
ACCESSION   DATROD010000167 DATROD010000000
VERSION     DATROD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.169"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..220)
                     /locus_tag="VBC74_001980"
     CDS             complement(<1..220)
                     /locus_tag="VBC74_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEO6942876.1"
                     /translation="MKNNIIFSIEQYINDQEMPKGKQKVILSAIELFASQGFHGTSTA
                     QLAKNAEVSQATIYKYFETKDKLLVFILE"
     gene            complement(244..1341)
                     /locus_tag="VBC74_001981"
     CDS             complement(244..1341)
                     /locus_tag="VBC74_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6942877.1"
                     /translation="MRIIAITEKVIKELFRDKRTLAMMFLAPILIMFLMNVMFSANSN
                     TKVKIGTINVNTKVVSNLDNIKHIQVRSFKFNSSAKKALKSNKIDALISEDNKSYTVF
                     YANTDSSKTTLTRQAFKTAVNTMNSKELISQVKILANKNPKLAQSLQTRSKYIKEKYN
                     YGNKNTGFFAKMIPILMGFMVFFFVFLISGMALLKERTSGTLDRLLATPVKRSDIVFG
                     YMLSYGILAIIQTIVIVLSTIWLLDIQVVGSIFSVIIVNFILALVALSLGILMSTLAK
                     SEFQMMQFIPLIIMPQLFFSGIIPLENMASWAQTVGKILPLSYSGDALTKIIMYGQGL
                     PNVSSNLLVLLLFLIILTIANIFGLKRYRKV"
     gene            complement(1344..2060)
                     /locus_tag="VBC74_001982"
     CDS             complement(1344..2060)
                     /locus_tag="VBC74_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEO6942878.1"
                     /translation="MKKVIDLKKLQKAYASETVLNNINLEVFKGEIIGLIGPSGAGKS
                     TLIKTMLGMEKADKGTALVLDTQMPDRNILNQIGYMAQSDALHESLTGLENLLFFGKM
                     KGIQKTELKQQITHISKVVDLENQLDKFVSGYSGGMKRRLSLAIALLGNPTVLILDEP
                     TVGIDPSLRRKIWQELINIKDEGRSIFITTHVMDEAELTSKVALLLRGNIIAFDTPLH
                     LKKQFNVSTIEEVFLKAEGE"
BASE COUNT          702 a          342 c          346 g          765 t
ORIGIN      
        1 actcgagaat aaagaccaaa agcttgtctt tagtttcaaa atatttatat atagttgctt
       61 ggctaacttc tgcattttta gctagttgag cagtggaagt tccatggaag ccttgtgagg
      121 caaagagctc tatagcggat aaaataactt tttgttttcc ttttggcatc tcttggtcat
      181 taatatactg ttcaatagaa aaaataatat tatttttcat acttagttcc ttttcaaatg
      241 gtattatact ttacggtatc gttttagtcc aaagatattt gcgatggtta aaataattaa
      301 aaatagaaga agtaccagaa gatttgaaga aacgttaggt aatccttgac cgtacataat
      361 tatttttgta agtgcatcac cagagtagga tagcggtaga attttaccga ctgtctgagc
      421 ccaacttgcc atattttcta gagggattat tcctgaaaag aataattgag gcattataat
      481 taagggaata aattgcatca tttgaaattc tgattttgct agagtagaca ttaagattcc
      541 taatgataag gcaaccagag ccaaaataaa attgacaatg atgacactaa aaatactacc
      601 tacaacttga atatctagta gccaaatagt tgataaaacg ataactatcg tttgaataat
      661 tgctagaatg ccgtatgata acatatatcc aaatacaata tcactacgtt taacaggagt
      721 tgcaaggagt cgatcaagtg taccagaagt acgctctttt aataaagcca ttccagaaat
      781 taaaaaaaca aagaagaaga ccataaatcc cattagtatt ggtatcattt ttgcaaaaaa
      841 gcctgtattt ttatttccgt aattatattt ttctttgata tatttggagc gagtttgtaa
      901 ggattgtgct agtttcggat tcttattagc taaaatttta acttgcgaaa tcagttcctt
      961 actgttcatt gtattaacag cggttttaaa agcttgtctt gttaaagtcg tctttgaaga
     1021 atctgtattc gcatagaaga cagtataaga tttattgtcc tccgaaataa gagcatcaat
     1081 tttatttgat ttgagtgctt ttttagcaga tgagttaaat ttaaatgatc tcacttgaat
     1141 atgcttaata ttatctaaat ttgaaacgac cttcgtgtta acgttaatag ttccaatctt
     1201 aacttttgta ttactattcg cagaaaacat aacattcatc aaaaacataa ttaaaatagg
     1261 tgctaaaaac atcatagcaa gtgttctttt atcacgaaac agttctttta taaccttttc
     1321 agttattgca ataattctca tgattattct ccttcagctt ttaagaaaac ttcctcaata
     1381 gtactcacat taaattgttt ttttaaatgt aatggagtat caaaggcaat aatgtttcca
     1441 cgtaatagta gtgcaacctt acttgttaat tctgcttcat ccataacgtg ggttgtaata
     1501 aagatagaac gtccttcatc cttaatatta attagctctt gccagatttt tctcctcaag
     1561 gatggatcaa ttccaacggt aggttcatct aggattaaaa ctgtggggtt tccaagtagg
     1621 gcgatggcta gagaaagccg tcttttcata cctcctgagt aacctgagac aaatttatca
     1681 agttggtttt ctagatctac tactttagaa atatgagtta tctgctgttt taattcagtt
     1741 ttttgaatac ctttcatttt tccaaagaat aataaatttt ctaagccagt taaagactcg
     1801 tgtaaggcat cagattgagc catatagcca atttgattta aaatattacg atctggcatt
     1861 tgagtatcaa gaacaagagc tgttccctta tctgcttttt ccatgccaag catagtttta
     1921 atcaaggtag atttccctgc tccagagggt cctattaatc caattatttc tcctttaaac
     1981 acctccaaat taatattatt taaaacagtt tctgaggcgt atgctttttg tagttttttt
     2041 aaatcgatga cttttttcat gataactttt tgctcctctc tcagtgagtg agtgtttact
     2101 caccaatttg atatcatata ttatagtgtg taaagacagg cttgtcaata aaaga
//
LOCUS       DATROD010000168         2117 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.170, whole genome shotgun sequence.
ACCESSION   DATROD010000168 DATROD010000000
VERSION     DATROD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.170"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..1280
                     /gene="rlmD"
                     /locus_tag="VBC74_001983"
     CDS             <1..1280
                     /gene="rlmD"
                     /locus_tag="VBC74_001983"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEO6942879.1"
                     /translation="KKTLIFVPGALKGEEVFCQISSVRRNFAEAKLLKINKKSKNRVD
                     PACSIYKECGGCQIMHLQYDKQLEFKTDIIRQALMKFKPEGYENYEIRKTIGMSEPEH
                     YRAKLQFQVRSFGGNVKAGLYAQGTHRLIDIKDCLVQDRLTQEMINRVAELLGKYKLP
                     IYNERKIAGVRTVMIRRAQASGEVQLIFITSKRLDFDDVVIELVREFPELKTVAVNIN
                     ASKTSDIYGQITEVIWGQESINEEVLDYGFSLSPRAFYQLNPKQTQILYSEAVKALDV
                     KEDDDLIDAYCGVGTIGLAFAGKVKSVRGMDIIPEAIQDAKENALHMGFTNTHYEAGK
                     AEDIIPRWYSEGFRANALIVDPPRTGLDDKLLNTILKMPPEKMVYVSCNTSTLARDLV
                     TLTKVYHVHYIQSVDMFPHTARTEAVVKLQRKV"
     gene            1548..1799
                     /locus_tag="VBC74_001984"
     CDS             1548..1799
                     /locus_tag="VBC74_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942880.1"
                     /translation="MIINSFDNSRKATINPEDINSPIKGFPKTVITCFARETFNRILE
                     ELPHREIARTSVANLEIPIYELEFKGQKIVLIKRLVMKI"
     gene            1796..2101
                     /locus_tag="VBC74_001985"
     CDS             1796..2101
                     /locus_tag="VBC74_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6942881.1"
                     /translation="MICLHGMNWVDGQLAAYYRLIPEDDKVHLGRVIVNPDFRKKGLG
                     NQLVEYEIKFSEANYPNKPIYAQAQAYLQDFYQSFGFQPISDIYLEDNIPHLDMVLS"
BASE COUNT          731 a          291 c          421 g          674 t
ORIGIN      
        1 ataaaaaaac actaatcttt gttccagggg ctctcaaggg agaagaggtg ttttgtcaga
       61 tttcttctgt tagacgtaat tttgcagaag ctaaactgtt aaagataaat aaaaagtcta
      121 aaaatagggt agatcccgct tgttctattt ataaagaatg tgggggatgc caaataatgc
      181 accttcaata tgataagcag ctagagttta aaacggatat tattcgacag gctctaatga
      241 aatttaaacc agagggatat gagaattatg aaatccgtaa aacgataggt atgtcggaac
      301 ctgaacacta tcgtgctaaa ttacaatttc aagtgcgttc gtttggaggg aatgtaaagg
      361 caggtttata tgcacaaggg acacatcgat tgattgatat caaagattgt ttagtgcaag
      421 ataggcttac tcaggaaatg ataaatcgag ttgcagaact tttaggaaaa tataaattgc
      481 ctatttataa tgaacgtaaa atagctggtg ttcgtactgt tatgattcga cgagcacaag
      541 cgagtggaga ggttcaactg atttttatca caagtaaaag acttgatttt gatgatgttg
      601 ttattgaatt agttcgagag tttcctgagt taaagacagt tgctgtaaat atcaacgcta
      661 gtaaaacaag tgatatctat ggtcaaataa cagaagttat ttggggtcaa gaatccatca
      721 atgaagaagt attagattat ggtttttcgt tatcaccacg tgctttttat caacttaatc
      781 ctaaacaaac acaaatttta tatagtgagg cagtcaaggc cttagatgtg aaggaagatg
      841 atgatttaat tgacgcttat tgtggagtag gaacgattgg acttgctttt gctggaaaag
      901 ttaaaagtgt tcgtggaatg gatattatcc ctgaggctat tcaggatgct aaagagaacg
      961 cacttcatat gggttttact aatacacatt acgaagctgg aaaggcagag gatattatcc
     1021 ctaggtggta tagtgaggga tttcgcgcta atgcgttaat cgtggatcct cctcgcacag
     1081 gcttagatga taaattgcta aatactatac taaaaatgcc tccagaaaaa atggtttacg
     1141 tttcttgtaa tacatctacg cttgctagag acttagtaac attaacaaaa gtataccatg
     1201 ttcactacat ccaatctgtt gacatgtttc cacatacagc aaggaccgag gctgtagtga
     1261 aactacaacg taaggtataa taaagttttc taatctaaaa ataagagtat ttttgggtat
     1321 tcttattttt agatagtaat ggtgagatat taatatagca tagtataaaa cacatggttt
     1381 aagctatttg atacggaaat ttaccttaga aatggtaaat aattccatta agtatattca
     1441 tctttgcaaa tttgaccttg ccttttaagt ttcattgggt taaaatagaa aaaatatttt
     1501 taacaagatg atagaacatc gtaaattaag gtttaaagga aatgcttatg ataataaatt
     1561 cattcgataa tagtagaaaa gcgactatta atcctgaaga tattaattca ccaataaaag
     1621 gttttccaaa gactgttatt acctgttttg cgcgggagac tttcaatcgc attttagaag
     1681 aattgcctca tcgtgaaatt gctagaacta gtgtagcaaa cttagaaatt cccatttatg
     1741 aattagaatt taaaggacaa aagattgtcc ttatcaagag gttggtgatg aagatttgat
     1801 ttgtttgcat gggatgaatt gggtagatgg tcaattggca gcttattata gattgatacc
     1861 agaagatgat aaggtgcatt tgggacgtgt gattgttaat ccagacttta gaaaaaaggg
     1921 attaggaaat caattagtgg aatatgagat taaatttagc gaggcaaatt accctaataa
     1981 acctatttat gctcaggccc aagcttattt acaagatttt tatcaatctt tcggatttca
     2041 acctatctca gatatctatc tagaagataa tattcctcat cttgatatgg ttttatcata
     2101 aatacatact atttaaa
//
LOCUS       DATROD010000169         2023 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.171, whole genome shotgun sequence.
ACCESSION   DATROD010000169 DATROD010000000
VERSION     DATROD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.171"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            1..1083
                     /locus_tag="VBC74_001986"
     CDS             1..1083
                     /locus_tag="VBC74_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001868100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEO6942882.1"
                     /translation="MERFIIVKINEVKKKDGTVIYRASVYLGTDKVTGKKVTTKITGR
                     TKKEVREKAKQGAVEFIKNGSTRFKATSVTSYQELATLWWDSYKHTVKYNTQLADKTN
                     KGERKAYLHYDRIHALNKRILQYGVIMQAIPFNPAREVILPRNTKKANTKRVKHFEND
                     ELRTFFNYLNNLDKSKYRNFYEVTLYKFLLATGCRINEALALNWSDIDLDNAVVHITK
                     TLNYKQEINSPKSKSSYRDIDIDSQTITMLKQYRRRQIQEAWKLGRSETVVFSDFIHK
                     YPNNRTLQTRLRTHFKRANVPNIGFHGFRHTHASLLLNTGIPYKELQYRLGHSTLSMT
                     MDIYSHLSKENAKKAVSFFETAINSI"
     gene            complement(1191..1802)
                     /gene="rpsD"
                     /locus_tag="VBC74_001987"
     CDS             complement(1191..1802)
                     /gene="rpsD"
                     /locus_tag="VBC74_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEO6942883.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKAKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
BASE COUNT          729 a          360 c          362 g          572 t
ORIGIN      
        1 atggaaaggt ttataatcgt gaaaataaat gaagtaaaaa agaaagatgg tacggtaatt
       61 tatcgtgcca gtgtttatct tggtactgat aaagtaacag gtaagaaagt aaccactaaa
      121 ataacaggac gaactaagaa agaagttaga gaaaaagcta agcaaggagc tgttgagttt
      181 ataaaaaatg gttctactcg cttcaaagct acttccgtaa caagctatca ggaacttgca
      241 accttatggt gggatagtta caaacatacc gtaaaataca atactcagct agctgataaa
      301 actaataaag gagaaagaaa agcttatctc cattatgaca gaatacacgc gctaaataaa
      361 cgtatactac aatatggtgt tatcatgcaa gctataccat tcaaccctgc tcgtgaggtc
      421 attctccctc gcaacactaa gaaagctaac actaaaagag taaagcattt tgaaaatgat
      481 gagctaagaa catttttcaa ctacttaaac aatctagata aaagtaaata tagaaacttc
      541 tacgaagtca cactttataa gtttttgtta gctacaggtt gtcgcattaa tgaagcgtta
      601 gctctaaact ggtcagatat cgacttggat aatgccgttg ttcatatcac aaaaacgcta
      661 aattacaagc aagaaattaa tagtccaaag tcaaagtcaa gctatcgtga tattgacata
      721 gatagtcaaa caattacaat gcttaagcag tatagacgaa gacagattca agaagcatgg
      781 aagttaggtc gttcagaaac agtggtattt tctgatttta tccataagta ccccaacaat
      841 agaaccttac aaactcgatt aagaacacat tttaaaagag cgaatgtacc gaatataggc
      901 tttcatggtt ttcgtcacac tcacgctagt ttattgctca acacaggtat cccctacaaa
      961 gaactccaat ataggttagg gcactctact ctatctatga ctatggatat ttatagccat
     1021 ttatcaaaag agaatgcaaa aaaagctgtc tcattctttg aaacagcaat taactcaata
     1081 tagcaaaaaa atcaaaagtt agcaaaaaag taagcaaacc cagaaatcaa tgttttaaga
     1141 taagagaaaa agcccactgt tgtaggcttt cagtaagttt aatcttaaaa ttaaagcatt
     1201 ttgttgtaga attcaacgac aagtgcttca ttgatttctg ggttaatttc atcgcgttct
     1261 ggaaggcgag tcaatgaacc ttcaagtttt tcagcatcaa atgatacgaa agctggacgt
     1321 cctaaagtag cttcaacagc ttcaaggatt gcaggtactt tcattgattt ttcacgaact
     1381 gaaataactt gtcctggagt aacgcggtat gatgggatat caacacgttt accatcaaca
     1441 aggatatgac catggttaac aaattgacga gcttggcgac gagttgttgc aagtcctaaa
     1501 cgataaacaa cgttatcaag acgacgctct aaaagaacca taaagttgaa accaagagtt
     1561 ccttcttttg ctttagtagc ttgtacgaac aagttacgga attgtttttc accaagtccg
     1621 tatgaaaaac gaagtttttg tttctcagct aactgcaagc catattctga aagcttgcta
     1681 cggttattag gaccgtgttg accaggtacg tagttacgac gtgccaattc tttacctgtg
     1741 cctgtaagtg ataaaccaag gcgacgtgat tgtttccatg atggaccagt ataacgtgac
     1801 atctaaatgt cctcctctaa aataattgag gaaataacga tttaaaaaat cctgattcgt
     1861 gcaggggatt ttcgcctaaa cagcaaaggg tactagatta gttaaccacc tgttgacgag
     1921 cttcatattt ttctgctgtt atttcacaca agctatatta tatcatgaaa ataatattga
     1981 atcaagtttt tatgtctctt aaaccttaaa tacaaataga cat
//
LOCUS       DATROD010000170         1788 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.172, whole genome shotgun sequence.
ACCESSION   DATROD010000170 DATROD010000000
VERSION     DATROD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.172"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            51..251
                     /locus_tag="VBC74_001988"
     CDS             51..251
                     /locus_tag="VBC74_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942884.1"
                     /translation="MNSEIRNRKHVYLNDDIIFQLAEISEFLKSSQSTFKQERSPYSQ
                     IIDLRKEEAQAKSVRNSIEKLL"
     gene            382..1335
                     /locus_tag="VBC74_001989"
     CDS             382..1335
                     /locus_tag="VBC74_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HEO6942885.1"
                     /translation="MKICILGLGVIGTTYGYIFQKAGHQVEHLVRESKKQDVPLKLDI
                     TMLDGRYNNKGEEKTDIYTVNLAKPNTNYDFILLSVAGGRIKDAIATLSQNNITGSLL
                     LFCNFWNSREEIEKMVGDFKYIIGFPTAGGNLKQKKLNCVLFDHIMLESEEKATITNY
                     KELLEFLESADIKVENPYDMIEWIWIHMAINAGVTSTAARAGKIDDPKQLAVNLMRDN
                     HGLSIAVKSIRETIKVVKNRGVDLRLYKNELFPYKIPSTIAGIIMKRMFARNELTSRI
                     MTLHNDVKDIMYGCKCIYDAGKQEKLNLPLFYHNMDKIFNS"
BASE COUNT          711 a          193 c          327 g          557 t
ORIGIN      
        1 acattaatta tttaacaaga tattccgtta aggaaaatta aaggagaaaa atgaattcag
       61 aaattcgaaa tagaaaacat gtttatttga atgatgatat tatctttcag ttagctgaaa
      121 tctcagaatt tttaaagtca agtcagtcaa catttaaaca ggagcgtagt ccatattctc
      181 aaataataga cctacgaaaa gaagaagcac aagctaaaag tgttaggaat agcatagaaa
      241 aattattgta agaaaacagt gatctaatac aggtaaggaa gtatgagtta ggctataaaa
      301 ctaagtaaaa aaatatgata taatataaaa aattatcgag taaaataaac tggaagcgaa
      361 acttaaggag atgattatta aatgaaaata tgtattttag gattaggcgt aataggcaca
      421 acttatggat atatctttca aaaagcagga catcaggttg aacatttagt tagagaaagt
      481 aaaaagcaag atgttccatt aaagctggat ataaccatgt tagatgggcg atataacaat
      541 aagggagaag aaaaaacaga tatatatact gtgaatcttg caaaaccaaa tacaaattat
      601 gactttatcc ttttaagtgt tgcaggcggg aggataaaag atgccatagc aacactttca
      661 caaaataata ttacaggaag tttgttatta ttttgtaact tttggaacag ccgtgaagaa
      721 atcgaaaaaa tggttggtga ttttaaatat atcattggat ttccgacagc aggtgggaat
      781 ttgaagcaga aaaagttgaa ttgtgttctg tttgaccata taatgcttga aagtgaagaa
      841 aaagcaacta tcacaaacta taaagagttg ttagaatttc tcgaatctgc cgacataaaa
      901 gtagaaaatc cttatgatat gatagagtgg atatggattc atatggcaat aaatgcaggt
      961 gttacttcta ctgcagcaag ggcaggaaaa attgatgatc caaaacaact tgcggttaat
     1021 cttatgagag ataatcatgg attgtctatc gcggtaaaat caataagaga aacaattaaa
     1081 gtagtaaaaa atagaggggt cgatttaaga ttatataaaa atgaactttt tccttataaa
     1141 ataccttcta ctattgcagg aataattatg aaacgaatgt tcgcaagaaa tgaacttacg
     1201 agtagaatta tgacgttgca caatgatgta aaagatatta tgtatggttg taaatgtatc
     1261 tatgatgctg gaaaacagga aaaattgaac ttaccattgt tttatcataa tatggataaa
     1321 atatttaata gttaaatcca tattcataaa tcagtgtttg gagggagaat tttttaaatg
     1381 gttttagtga tttatatttt attatgattt actagtttca gttacaatga ttattgacgg
     1441 acgaatgatg tggaatcatc tacctgaaaa aattaataga ttaatgggat atcgtactac
     1501 tttatcaatg aaaaaatagg tacatggagt tttatacatg attactgtgg ctattaatta
     1561 ttattcatat tctattttat agaagcacag atgatgaaat aggtattatt agcttaattg
     1621 tagtttctat acgaataact attttaattg tgttaatact tacgactgaa cgggagttga
     1681 agaaaacttt cacagaaaat tgcattagaa agtagaacga tagcttctag cttgattaat
     1741 tgaatagaat atttttagaa atagtaaatc acaaaggttt actatttt
//
LOCUS       DATROD010000171         1758 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.173, whole genome shotgun sequence.
ACCESSION   DATROD010000171 DATROD010000000
VERSION     DATROD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.173"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..1740
                     /gene="uvrC"
                     /locus_tag="VBC74_001990"
     CDS             <1..1740
                     /gene="uvrC"
                     /locus_tag="VBC74_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEO6942886.1"
                     /translation="SPGCYLHKDKNGTIIYVGKAKNLKNRVKSYFHGSHNTKTELLVS
                     EIEDFEYIVTTSNTEALLLEINLIQENMPKYNIRLKDDKSYPYIKITNERYPRLMITR
                     QVKKSDGTYFGPYPDSGAATEIKRLLDRLFPFKKCTNPANKVCFYYHLGQCNAHTVCQ
                     TNKAYWDSLREDVKQFLNGKDNKIVNGLTEKMKSAAMTMEFERAAEYRDLIEAISLLR
                     TKQRVIHQDMKDRDVFGYFVDKGWMCVQVFFVRNGKLIQRDVNMFPYYNEPEEDFLTY
                     IGQFYQDTKHFLPKEVFIPQDIDAKSVETIVGCKIVKPQRGEKKQLVNLAIKNARVSL
                     QQKFDLLEKDIRKTHGAIENLGNLLNIPKPVRIEAFDNSNIQGTSPVAAMVVFVNGNP
                     SKKDYRKFKIKTVIGPDDYASMREVIHRRYSRVLKDGLTPPDLIVIDGGQGQVNIARD
                     VIENQLGLAIPIAGLQKNDKHQTHELLFGDPLEVVELPRNSEEFFLLHRIQDEVHRFA
                     ITFHRQLRSKNSFSSKLDGITGLGPKRKQLLMKHFKSLPNIQKADIEDIIMCGIPRTV
                     AESLRDSLNDPPK"
BASE COUNT          605 a          257 c          347 g          549 t
ORIGIN      
        1 agtcctggtt gttaccttca taaagataag aatgggacaa ttatttatgt aggtaaggct
       61 aaaaatctta agaatcgtgt gaaaagttat tttcatggga gtcataatac taagacagaa
      121 cttttggtct ctgagattga agactttgaa tacattgtga caacatctaa taccgaagct
      181 ctattattgg aaattaattt aatccaagaa aatatgccga agtataatat tcgattgaaa
      241 gatgataagt cttacccata tatcaaaata acaaatgaac gttatcctcg tcttatgatt
      301 acacgtcaag taaaaaaatc agatggcact tattttggac cttatccgga ttctggggca
      361 gcaactgaga ttaagcggtt actagatagg ctctttcctt ttaagaaatg tactaatcca
      421 gcaaataaag tatgctttta ttatcattta gggcaatgca atgcacatac agtttgtcaa
      481 actaataaag cttattggga tagtctgaga gaagatgtaa aacagtttct taatggtaaa
      541 gataataaaa ttgttaacgg attaactgaa aaaatgaaga gcgctgctat gactatggaa
      601 tttgagcgtg cggctgaata tcgagatttg atcgaagcaa ttagtttgtt gcgtactaag
      661 cagcgtgtta ttcatcaaga tatgaaggat agagatgttt ttggttattt cgttgataag
      721 ggttggatgt gtgtacaggt cttttttgtt cgaaatggaa aattgattca gcgtgacgtg
      781 aatatgtttc cttattataa tgaaccagag gaagattttc taacttacat agggcaattt
      841 tatcaagata caaaacattt tctacccaaa gaagtcttta ttccacagga tattgatgct
      901 aagtcggttg aaacaattgt aggctgtaag attgttaaac cacaacgtgg tgaaaagaaa
      961 cagttagtaa atctggcaat aaaaaatgct cgtgtcagtt tacaacagaa gtttgactta
     1021 ctggaaaaag atattcgtaa aactcatggg gcaattgaaa atttaggtaa tcttcttaat
     1081 atccctaaac cagtacgcat agaagcattt gataattcca atattcaagg gacaagtcca
     1141 gtagctgcta tggtggtatt tgtgaatgga aatccaagta aaaaagacta tcgaaagttt
     1201 aaaattaaga ctgttatcgg accagatgat tatgctagta tgagagaagt cattcatcgg
     1261 cgttatagta gggttttaaa agatggcttg acacctccgg acttgattgt aattgatggt
     1321 ggtcaggggc aagtaaatat agcgcgtgat gttattgaga accaattagg tttagccata
     1381 cctattgcag gtttgcaaaa gaatgataaa caccaaacac atgaattact ttttggagat
     1441 cctttagaag tagtagaatt accgcgtaat tcagaagaat ttttcttact tcaccgtatt
     1501 caagatgagg tacatcgttt tgctattact tttcatcgtc aattacgctc caaaaattcc
     1561 ttttcttcaa aattagatgg tatcactggg ttgggaccaa aacgtaaaca attgctaatg
     1621 aaacatttta aaagcttacc taatatccaa aaagctgata ttgaggacat tattatgtgt
     1681 ggtattccta ggacagttgc agaaagtctc cgagattctc ttaatgatcc tcctaaataa
     1741 aaaacttagt agaacagt
//
LOCUS       DATROD010000172         1665 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.174, whole genome shotgun sequence.
ACCESSION   DATROD010000172 DATROD010000000
VERSION     DATROD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.174"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(435..653)
                     /locus_tag="VBC74_001991"
                     /pseudo
     CDS             complement(435..653)
                     /locus_tag="VBC74_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001889697.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          536 a          310 c          201 g          618 t
ORIGIN      
        1 ttttacatat ttattaatta atatggtaac acccattatc tgaagtgtgt aggcgaggtc
       61 taagtaaaga atttaagcgt tttgataata aaaaagtgag aacaatagat tctcactcac
      121 aattagcctg gtaatttctc acacgatttg gtactgcttt ttttaagttg ggcgatggtg
      181 ttatttttaa tatgagtagc tgcactcgaa tccgattgct caaaagcact tgtcgctcca
      241 ttgagatagc tattcagatc agaaataact ggttgaatgt tttggctgac ttctttgata
      301 acttttgatc cagcttctaa gtattttcaa tgttcttgaa cccattggtg atcttttttt
      361 actaagtcac atgctgttaa aatcatcatt aagcttatcc cttccacaat aataatcaat
      421 tttttaaatc ctttttatct tattgatttt ttcaatttta ttgctgtctt gacgttgcta
      481 tcgcaaacaa ccaactcaat gcaatatagt tacggtatac atatcaatgt aagactcaag
      541 atttttaaat tcatattatt tgtaggttct ttatcagacc aagctaatac tctttcattt
      601 gaagtaatat tattatttgt atgatattca agattagcta tttttgattt cacaaaaaaa
      661 ccttcttcta cttttttaga ctgattcttt cttcccactt cttcgtatca atatcatata
      721 tttgaagaac taaactctca gagtttccag tcaattttag taataatccc aaaactaatc
      781 cttcctacaa accaacggat aattttattt caactggtcc tggttttaat tttacttgtg
      841 caatttcgct tgttatacca actattgata tcgccgatac tcctattcct aatgatgcgc
      901 acgcatcatt agttaaacaa agtactttaa atcctacaga gggtcattcc atcaattaat
      961 ggcataggtt ttggcactag aaactgaata tgaattctat tatctattaa aaaaatcaat
     1021 atcaccagag tatttataaa acataacgat aaaaaatcaa aaaatatttt tactatttcc
     1081 cttaatttca tgagatttaa tttccctttt tgtgtattat ttctattcta caatagtctt
     1141 acccttagaa atatttttcc atttttcaat gatacgagct actctttctt actcctcgtc
     1201 atggtacctc ccaaaattac ttcatcaata tcttctcatt gaaataatat acttgttcta
     1261 aattgacctc tctggaaaaa tcctgccaga taatcaaaat ataatcttga tcaataatat
     1321 ttgctcatga aacggctcca caaaatttct tacatcttta acattttcat agagtgcttt
     1381 tactccacaa tggccaaagt acctataaca actgctacta ctggtacaaa ttggagttgt
     1441 tatgattact acaaaaacac tctcagcaag cgtagagact attctaccca cttcattttc
     1501 tctagatatt tattgctcct accttaaatt aattaattta tcactcatat tcgggatatt
     1561 tatgaatata atatgctgca taaagggaat cgacaatcag agctgtaaaa gttgccgtaa
     1621 ataatacacc tccaatcaaa ccaaaaatat tttcaatact accta
//
LOCUS       DATROD010000173         1664 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.175, whole genome shotgun sequence.
ACCESSION   DATROD010000173 DATROD010000000
VERSION     DATROD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.175"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..588
                     /locus_tag="VBC74_001992"
     CDS             <1..588
                     /locus_tag="VBC74_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEO6942887.1"
                     /translation="AKRFLDITGAIIGLLICGIVAIFLVPQIRKDGGPAIFSQNRVGR
                     NGRIFRFYKFRSMRVDAEQIKKDLLVHNQMTGLMFKLEDDPRITKIGKFIRKTSIDEL
                     PQFYNVLKGDMSLVGTRPPTVDEYEKYNSTQKRRLSFKPGITGLWQISGRNNITDFDE
                     IVKLDVQYINEWSIWSDIKIILLTLKVVLLGTGAK"
     gene            612..1061
                     /locus_tag="VBC74_001993"
     CDS             612..1061
                     /locus_tag="VBC74_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HEO6942888.1"
                     /translation="MKICLVGSSGGHLAHLNLLKPIWEKEDRFWVTFDKEDARSILRE
                     EIVYHCFFPTNRNVKNLVKNTILAFKVLRKERPDVIISSGAAVAVPFFYIGKLFGCKT
                     VYIEVFDRIDKPTLTGKLVYPVTDKFIVQWEEMKKVYPKAINLGGIF"
     gene            1061..1534
                     /locus_tag="VBC74_001994"
     CDS             1061..1534
                     /locus_tag="VBC74_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug MFS transporter"
                     /protein_id="HEO6942889.1"
                     /translation="MIFVTVGTHEQQFNRLIKEVDRLKGTGAIDQEVFIQTGYSDFEP
                     QNCQWSKFLSYDDMNSYMKEAEIVITHGGPATFMSVISLGKLPVVVPRRKQFGEHIND
                     HQIQFLKKIAHLYPLAWIEDVDGLAEALKRNIATEKYQGNNDMFCHKLEKIIGEI"
BASE COUNT          566 a          220 c          341 g          537 t
ORIGIN      
        1 gcaaaacgat ttttggatat cacgggtgct attataggtt tgctcatatg tggcattgtg
       61 gcaatttttc tagttccgca aatcagaaaa gatggtggac cggctatctt ttctcaaaat
      121 agagtaggtc gtaatggtag gatttttaga ttctataaat tcagatcaat gcgagtagat
      181 gcagaacaaa ttaagaaaga tttattagtt cacaatcaaa tgacagggct aatgtttaag
      241 ttagaagatg atcctagaat tactaaaata ggaaaattta ttcgaaaaac aagcatagat
      301 gagttgcctc aattctataa tgttttaaaa ggtgatatga gtttagtagg aacacgccct
      361 cccacagttg atgaatatga aaagtataat tcaacgcaga agcgacgcct tagttttaag
      421 ccaggaatca ctggtttgtg gcaaatatct ggtagaaata atattactga ttttgatgaa
      481 atcgtaaagt tagatgttca atatatcaat gaatggtcta tttggtcaga tattaagatt
      541 attctcctaa cactaaaggt agttttactc gggacaggag ctaagtaaag gtaaggtttg
      601 aaaggaatat aatgaaaatt tgtctggttg gttcaagtgg tggtcatcta gcacacttga
      661 accttttgaa acccatttgg gaaaaagaag ataggttttg ggtaaccttt gataaagaag
      721 atgctaggag tattctaaga gaagagattg tatatcattg cttctttcca acaaaccgta
      781 atgtcaaaaa cttggtaaaa aatactattc tagcttttaa ggtccttaga aaagaaagac
      841 cagatgttat catatcatct ggtgccgctg tagcagtacc attcttttat attggtaagt
      901 tatttggttg taagaccgtt tatatagagg ttttcgacag gatagataaa ccaactttga
      961 caggaaaatt agtgtatcct gtaacagata aatttattgt tcagtgggaa gaaatgaaaa
     1021 aagtttatcc taaggcaatt aatttaggag gaatttttta atgatttttg tcacagtggg
     1081 gacacatgaa cagcagttca accgtcttat taaagaagtt gatagattaa aagggacagg
     1141 tgctattgat caagaagtgt tcattcaaac gggttactca gacttcgaac ctcagaattg
     1201 tcagtggtca aaatttctct catatgatga tatgaactct tacatgaaag aagctgagat
     1261 tgttatcaca catggcggcc cagcgacgtt tatgtcagtt atttctttag ggaaattacc
     1321 agttgttgtt cctaggagaa agcagtttgg tgaacatatc aatgatcatc aaatacaatt
     1381 tttaaaaaaa attgcccacc tgtatccctt ggcttggatt gaagatgtag atggacttgc
     1441 ggaagcgttg aaaaggaata tagctacaga aaaatatcag ggaaataatg atatgttttg
     1501 tcataaatta gaaaaaatta taggtgaaat atgaggaaat atctagattt agattattct
     1561 ttattttatg ctctttgggt acttatttta gtaccaaacc aatggtatca gtttttaatt
     1621 attaccatta tagttctatt attactttgg aagagtgagt ttag
//
LOCUS       DATROD010000174         1607 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.176, whole genome shotgun sequence.
ACCESSION   DATROD010000174 DATROD010000000
VERSION     DATROD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.176"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            146..580
                     /gene="rplK"
                     /locus_tag="VBC74_001995"
     CDS             146..580
                     /gene="rplK"
                     /locus_tag="VBC74_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEO6942890.1"
                     /translation="MAKKVEKLVKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKE
                     FNARTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGEPNKTKVAT
                     ITRAQVQEIAETKMPDLNAANLESAMRMIEGTARSMGFTVTD"
     gene            684..1373
                     /gene="rplA"
                     /locus_tag="VBC74_001996"
     CDS             684..1373
                     /gene="rplA"
                     /locus_tag="VBC74_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEO6942891.1"
                     /translation="MAKKSKNLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     SYNLNIDVKKADQQIRGAMVLPAGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KIQGGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDDAKLVDNFKAFNDVIVKAKPATAKGTYITNLSITTTQ
                     GVGIKVDPNSL"
BASE COUNT          551 a          268 c          303 g          485 t
ORIGIN      
        1 aatttaaaat ataatgaaat taatggctta aaactattgc ttttatttat gattatagta
       61 taatggtaag gtatgttata aacatgcctg tgggaggtaa aaatttctaa ttaccgccaa
      121 aaccacaaca ggaggatttt taaaaatggc taaaaaagtt gaaaaacttg taaaacttgt
      181 aaaacttcaa attcctgctg gtaaagcaac tccagctcca ccagttggac cagcacttgg
      241 tcaagcagga atcaacatca tgggattcac taaagagttt aacgctcgta cagctgatca
      301 agctggtatg attattccag ttgttatctc agtatatgaa gacaaatcat ttgatttcat
      361 cactaaaaca ccaccagctg ctgttctttt gaaaaaagct gcaggtgttg aaaaaggatc
      421 aggtgaacct aacaaaacta aagttgcaac aattactcgt gcgcaagtac aagaaattgc
      481 tgaaactaaa atgccagatt taaacgctgc aaaccttgag tctgcaatgc gtatgatcga
      541 aggtactgct cgttctatgg gattcactgt tactgactaa tcagaaacac ccacataacc
      601 cgcaagactt catcgtagtt tgatgagtga cgtgggagat tttaaatcaa atcgattgac
      661 cacattacaa ggagaatttt aaaatggcta aaaaaagtaa aaacttacgt gctgctcttg
      721 agaaaattga cagcactaaa gcatacagcg tagaagaagc tgtagctctt gcaaaagaaa
      781 ctaactttgc taaatttgat gcaactgttg aagtttctta taaccttaac attgacgtta
      841 aaaaagctga ccaacaaatc cgtggtgcta tggttttacc agcaggtact ggtaaaactt
      901 cacgtgttct tgtttttgct cgtggtgcta aagctgaaga agctaaagca gcaggtgcag
      961 actttgtcgg tgaagatgac ttagttgcta aaatccaagg tggatggctt gactttgatg
     1021 ttgttatcgc aacaccagat atgatggcac ttgttggacg tcttggacgt gtccttggac
     1081 ctcgtaactt gatgccaaac cctaaaactg gtacagtaac tatggacgtt gctaaagcag
     1141 ttgaagagtc taaaggtggt aaaattactt accgtgctga taaagcaggt aacgtccaag
     1201 cccttattgg taaagtttca tttgatgatg ctaaattagt tgataacttc aaagcattta
     1261 atgatgttat tgttaaagct aaaccagcta ctgcaaaagg aacttacatc acaaaccttt
     1321 caattacaac aacacaaggt gttggtatca aagttgatcc taactcactt taatcaataa
     1381 aaaactagga attccttcct agttttattt attctatata cttaactagt taagtatata
     1441 ttttaataat caaaggatat tatatgtgtt tcttttgaaa aatagctttt tatctgtatt
     1501 aaccttctta aagactaatg aatgtgcgat gtgttttaag taacagatga gaataaggaa
     1561 aaatgaaatt ggcgatatgg tatgtgacta ttatgattat tctttag
//
LOCUS       DATROD010000175         1446 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.178, whole genome shotgun sequence.
ACCESSION   DATROD010000175 DATROD010000000
VERSION     DATROD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.178"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..121)
                     /locus_tag="VBC74_001997"
     CDS             complement(<1..121)
                     /locus_tag="VBC74_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6942892.1"
                     /translation="MRDFEELFDKVKPIVMKLRRNYFVQLWEYDDWIQEGRIVL"
     gene            complement(321..740)
                     /locus_tag="VBC74_001998"
     CDS             complement(321..740)
                     /locus_tag="VBC74_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HEO6942893.1"
                     /translation="MKMTVYFDGNFWLGLIEYDDDGDYKVFRYFFGKEPKDDDVFNFI
                     NHKLNDLIKKYEFVKTDISLKRTNEHKKSPKRMQREINREKRKPVVSTKAQLAMKTIH
                     MSIKNERQLSQKCKKNELRKHRYQLKQEKRYQKKKGH"
     gene            complement(960..1237)
                     /locus_tag="VBC74_001999"
                     /pseudo
     CDS             complement(960..1237)
                     /locus_tag="VBC74_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019769613.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
BASE COUNT          467 a          274 c          182 g          523 t
ORIGIN      
        1 aaagtacaat acgtccttct tgaatccaat catcatattc ccaaagttga acaaaataat
       61 ttcttctcag cttcattacg attggcttta ctttatcaaa caactcctca aaatctctca
      121 aaacaactta ttcctttctt aaacatatac taagtataaa aaaagtaagg tgataacaac
      181 atgacaatgg tgtcctttaa ggaaatgatt ttttcccaaa acacattaat tgagcattat
      241 cagactattt agtaactcat attagttaac atataaaaag cttgagatga tactctcaag
      301 ctttttatat gatgacacaa tcaatgccct tttttctttt ggtaacgttt ttcttgtttt
      361 aattggtacc gatgctttct taactcattt ttcttacatt tctgacttaa ttgacgttcg
      421 ttttttatac tcatatgaat agttttcatc gccaattgcg cttttgtaga tactactggc
      481 ttacgttttt ctctattaat ttctctttgc attcttttag gacttttttt gtgttcattc
      541 gtacgcttta gagaaatatc cgttttaaca aactcgtatt tttttatcaa atcattaagt
      601 ttatgattta taaaattaaa aacatcatca tctttaggtt cttttccaaa aaaataccga
      661 aaaactttgt aatcaccatc atcatcgtac tcaatcaaac ccaaccaaaa attaccatca
      721 aaatagactg tcattttcat acagccccct cctttaaata ttaaattgca atggacgacc
      781 tggagggaag gttactgacg ttcactgaac gcttgcagac taccaactgc aacgtgtttt
      841 tatgctataa cgattataac acagtacact atgattatcg ataaacgacc ttattaaagg
      901 tctatactat ttgtcagtct aaaaatttaa aagctgttaa gccaactcca gtaacgttat
      961 cattttttta tgattgttaa acgaggccaa atattattcc atttcttctt atccactcgc
     1021 ttcaaataaa cattataacg taatagatcc tcttcaaaat atggcgtagg actaacaatg
     1081 aaatttaata tttcctcttc accataagag ggaggtaaag ttcgagttga tttctatcat
     1141 ctaacctttc tcccactgca gtacactttt caggaaattt cgaaatagca tctttagagc
     1201 tagtatactt tggggtatta ggagaatgag tgttcatgta aaattcattt ttaagttccc
     1261 aatcgtactg tggatagtta tcctttaatt gttgttctaa taccaccgtt tcttcataag
     1321 aaatattttt atcaaaaaat acaacatcaa tgtctgatgt caatgtctca tttattccag
     1381 aaagtttatt ccatataaag tttctcaatg tgccagcaca cagccaacaa tcattcagtg
     1441 gaagag
//
LOCUS       DATROD010000176         1437 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.179, whole genome shotgun sequence.
ACCESSION   DATROD010000176 DATROD010000000
VERSION     DATROD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.179"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..662
                     /locus_tag="VBC74_002000"
     CDS             <1..662
                     /locus_tag="VBC74_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEO6942894.1"
                     /translation="NIFNLLKKLWKKKFLITFVAIAFATAGLFYSLFIVTPQYTSSTR
                     IYVINPNTPNNSITAQDLQAGSFLANDYKEIITSTDVLEKVISSEKLNYPSSQLLQKI
                     TVSILKDTRVISISVEDANPKMSQKLANSVREAAVSKIKAVTQVEDITTLEKGNLPKA
                     PSSPNIKKNVLIGFIVGAGLSTIVLVIMGILDDRVNTEEDIEKVLGLTSLGIVPDLNK
                     L"
     gene            673..1362
                     /locus_tag="VBC74_002001"
     CDS             673..1362
                     /locus_tag="VBC74_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEO6942895.1"
                     /translation="MTRLEIVDSKLRQAKKTEEYFNAIRTNIQFSGKENKILAIASVR
                     EGEGKSTTSTSLALSLAQAGFKTLLIDADTRNSVMSGTFKATGTIKGLTNYLSGNADL
                     GDIICETNVPRLMVVPSGKVPPNPTALLQNAYFNKMIEAIKNIFDYIIIDTPPIGLVV
                     DAAIIANACDGFILVTQAGRIKRNYVEKAKEQMEQSGSKFLGIILNKVNESVATYGDY
                     GNYGKRDRKRK"
BASE COUNT          519 a          208 c          257 g          453 t
ORIGIN      
        1 taaatatatt caacctttta aaaaaattgt ggaaaaagaa atttttaatc acatttgttg
       61 ccattgcgtt tgcaacagct ggacttttct atagtctttt tattgttacg ccacaatata
      121 cttcttcaac aaggatatat gtcattaacc ctaatacccc taataatagt attactgcac
      181 aagatctaca agcggggagt tttcttgcaa atgactataa ggagattatt acgtctactg
      241 acgttctaga aaaagttatt tcttctgaaa aattgaatta tccttcgtct cagttgctac
      301 aaaaaataac agtttctatt ttaaaagata cacgtgttat ttcaatatcg gtcgaagatg
      361 ctaatccaaa aatgtctcaa aaattagcaa attcagttag agaagcagca gtttcaaaaa
      421 tcaaggcagt tactcaagta gaggatatca ctactcttga gaagggaaat ttacctaaag
      481 caccatcttc tcctaatatt aaaaagaatg tactaatcgg gtttattgtt ggtgcaggat
      541 tatcaactat tgttttagtt attatgggga ttttggatga tcgtgttaat actgaagaag
      601 atattgaaaa ggtcttagga cttacttctt taggtatagt accagatttg aataaacttt
      661 aaggagaata taatgactcg tttagaaata gttgatagca agttaagaca agcaaaaaaa
      721 acagaagaat acttcaatgc gatccgtaca aacatacagt ttagtggaaa ggaaaataaa
      781 attcttgcaa ttgcctctgt tagggaaggg gaaggaaaat ccactacttc aacaagttta
      841 gctttatctt tagctcaagc aggatttaaa acattattaa ttgatgcgga tactaggaac
      901 tctgttatgt ctggaacctt taaagcaact ggaactatta aaggcttgac gaattattta
      961 tcaggtaatg cagatcttgg agatattatc tgtgaaacca atgttcctag actgatggtc
     1021 gttccttcag gaaaagtacc accaaatcca acagcattac ttcagaacgc ttattttaat
     1081 aagatgattg aagctattaa aaatatattt gattatatta tcatcgatac tccacctatt
     1141 ggtttagttg ttgatgccgc aataatcgct aatgcttgcg atggttttat tttagtaacc
     1201 caagcaggca gaataaaacg taattatgtt gaaaaagcaa aagaacagat ggaacaaagt
     1261 ggttcaaagt tcttaggtat tattcttaat aaagttaatg aatctgttgc tacttacggc
     1321 gattatggaa attacggaaa aagggataga aaaaggaagt aagggactct ggtattgaaa
     1381 gaaaaagaaa atatacaaaa gattattata gcgatgattc aaacagttgt agtttat
//
LOCUS       DATROD010000177         1429 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.180, whole genome shotgun sequence.
ACCESSION   DATROD010000177 DATROD010000000
VERSION     DATROD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.180"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..701
                     /gene="recX"
                     /locus_tag="VBC74_002002"
     CDS             <1..701
                     /gene="recX"
                     /locus_tag="VBC74_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEO6942896.1"
                     /translation="EDTIVHFMLSKGMIINAEKLENIKKFAQLSYGKNLGLYYISFKQ
                     RTEKEVIKYLQEHDIDSKIIPQIIDNLKSENWINDKNYVQSFIQQNLNNGDKGPYVIK
                     QKLLQKGIKSKIIESELKAINFQDLASKISQKLYKKYQNKLPLKALKDKLMQSLTTKG
                     FDYQIGHTVIQNLEIEKDQELEDDLIYKELDKQYQKLSKKHDQYELKQRIINVLMRKG
                     YQYEDIKSALREYL"
     gene            785..1318
                     /locus_tag="VBC74_002003"
     CDS             785..1318
                     /locus_tag="VBC74_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEO6942897.1"
                     /translation="MRLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTENDGRRWVTREPAIVYFHKKYWFNIIAMIRETGVSYYCNLASPYILDPEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEQHKAQMNYPTDIDYILKENVKILVEWINENKGPFS
                     SSYINIWYKRYLELKKR"
BASE COUNT          595 a          207 c          221 g          406 t
ORIGIN      
        1 cagaagatac tatcgttcat tttatgttaa gtaaagggat gataattaat gccgagaaac
       61 ttgaaaatat taaaaaattt gcccaactat catacggtaa aaatctaggt ctttattata
      121 tttctttcaa gcagagaacc gagaaagaag ttattaaata tcttcaagaa catgatattg
      181 attctaaaat tattccacaa attattgata atctaaaaag tgagaattgg ataaacgata
      241 aaaactacgt gcaatctttc atacagcaaa atctaaataa tggcgataag gggccatacg
      301 tgataaaaca aaaattattg caaaaaggta tcaaaagtaa aataattgaa tctgaactta
      361 aagctataaa ctttcaagac ttagcatcca aaataagcca aaaactctat aaaaaatatc
      421 aaaacaaact tccactaaaa gcattaaaag ataaattgat gcagagctta actacaaaag
      481 gatttgatta tcagataggg cataccgtaa ttcaaaacct tgaaattgaa aaagatcaag
      541 aacttgaaga tgacctcatt tataaagaat tagataaaca ataccaaaaa ttatcaaaaa
      601 aacatgacca gtatgaatta aagcaacgta taatcaatgt cttaatgcgt aaaggatacc
      661 agtacgaaga tatcaaaagt gctttaagag aatatttata agataataac tctcaaacga
      721 tgtattttac agaaaattat gataaactat aacagacgta taaattgtag aaagttggta
      781 ggctatgaga ttacctaaag aaggcgactt tattacaatt caaagttaca aacatgatgg
      841 tagtttacac cgaacttggc gtgacaccat ggtattaaaa acaaccgaaa atgccctcat
      901 tggtgttaat gatcatactt tagtaacaga aaatgatggt cgacgctggg tgacacgaga
      961 gcctgcaata gtatactttc ataaaaaata ttggtttaac attatcgcta tgatacgtga
     1021 aactggtgtc tcctactatt gtaatctagc aagtccgtat atcttggatc ctgaagcact
     1081 caagtacatc gactacgacc ttgatgtcaa agtatttgca gatggtgaaa aaagactact
     1141 ggatgtggac gaatatgaac agcataaagc tcagatgaac tatcctaccg atattgatta
     1201 tatattaaag gaaaatgtaa aaatattggt agaatggata aatgagaata aaggcccctt
     1261 ttcatcatca tatatcaata tctggtataa acggtacctt gaattgaaaa agcgctaact
     1321 aacacactaa atagtgtgtt tttattaata tcaaatttaa ttacaatact attgcaaaaa
     1381 tatatactta aaataaaaaa agtagaaagt gatctttcta cttttttaa
//
LOCUS       DATROD010000178         1414 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.181, whole genome shotgun sequence.
ACCESSION   DATROD010000178 DATROD010000000
VERSION     DATROD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.181"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..704)
                     /locus_tag="VBC74_002004"
     CDS             complement(<1..704)
                     /locus_tag="VBC74_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEO6942898.1"
                     /translation="MIDIHSHIVFDVDDGPKTLEESLSLIEESYRQGVRIIVSTSHRR
                     KGMFETPEDIIFKNFSIVKHEAEKRFEHLQILYGGELYYTSDMLEKLKLKQIPTLNNT
                     KFALIEFSMQTSWKDIHTALSNVLMLGITPVVAHIERYNALENQKERVKEIINMGCYT
                     QINSFHILKQKLFNDKHKRFKKRARYFLEENLVHFVASDMHNLDVRPPFLAEAYKIIC
                     RDFGKERANQLFIENA"
     gene            complement(710..>1414)
                     /locus_tag="VBC74_002005"
     CDS             complement(710..>1414)
                     /locus_tag="VBC74_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEO6942899.1"
                     /translation="NIIMTVNTNTHKVLLTTTPRDAYVKIPDGGGNQYDKLTHAGLYG
                     VETSMKTLENLYDINLDYYARINFSSFLKLIDLLGGVTVYNDQAFTSKHGNFDFPVGQ
                     VTLNSEQALGFVRERYSLQGGDNDRGRNQEKVIAAIINKLASSQSVTKLNSITSQLQT
                     SVQTNMTIDNINDLINNQLSTGQRFTVESQALTGHGSTGELPSYAMPGAQLYMMSIDQ
                     SSLSNAKSKIKNTMEE"
BASE COUNT          438 a          251 c          213 g          512 t
ORIGIN      
        1 tgagcatttt caataaaaag ttggttagca cgttctttac cgaaatctct acagataatc
       61 ttataagctt ctgctaaaaa tggcggtcta acatcaaggt tatgcatatc actcgctaca
      121 aaatgcacta aattttcctc taaaaaataa cgggctcttt tcttaaagcg tttatgctta
      181 tcattaaaaa gtttttgttt caaaatatgg aaactattta tttgtgtgta acaccccata
      241 ttaataattt ccttcacccg ttctttttga ttctctaaag cgttatacct ctctatatgc
      301 gcaacgactg gtgtaatacc aagcattaaa acatttgaca aagctgtatg aatatctttc
      361 caagaagttt gcatagaaaa ttcaattaaa gcaaatttcg tattgtttaa agttggaatt
      421 tgctttaact tcagtttttc taacatatca cttgtatagt ataattctcc accatataag
      481 atttgaagat gttcaaatct tttttccgct tcgtgtttaa ctatagaaaa gtttttgaag
      541 ataatatctt caggtgtttc aaacatacct tttcttctat gtgatgtcga aacaattatt
      601 ctaacacctt gtcgataact ttcttctatt aaagataaac tctcttcaag cgttttgggc
      661 ccatcatcta catcaaaaac gatatgagaa tgaatatcaa tcattcttgt tattcctcca
      721 ttgtgttctt aatttttgat tttgcattag ataagctaga ttgatcaatt gacatcatat
      781 aaagttgagc tcctggcatt gcatatgaag ggagttcacc cgttgaacca tgaccagtta
      841 atgcttgtga ctcgacagtg aagcgttgtc cagttgacaa ttgattgtta atcaaatcat
      901 taatattatc aatagtcata ttagtttgaa cggacgtttg gagctgtgag gtaatgctat
      961 ttaattttgt tactgactga ctagaagcta acttatttat aatagctgca atcacttttt
     1021 cttgatttct acctctatcg ttatcgcctc cttgtagaga ataacgttct ctaacaaagc
     1081 ccaaagcctg ctcagaattc aatgttactt gaccaacagg aaagtcaaaa ttaccatgtt
     1141 tacttgtaaa agcttgatcg ttataaactg tcactcctcc caagaggtct attaatttta
     1201 aaaatgatga aaaattaatt ctagcataat aatcaaggtt gatgtcgtaa aggttttcaa
     1261 gtgttttcat tgatgtctca acaccataca aacctgcatg ggttaattta tcatattgat
     1321 tgcccccacc atctggaatt tttacatagg catctcgtgg tgttgtcgtt aacaatactt
     1381 tgtgggtatt ggtattaacc gtcataataa tatt
//
LOCUS       DATROD010000179         1402 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.182, whole genome shotgun sequence.
ACCESSION   DATROD010000179 DATROD010000000
VERSION     DATROD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.182"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            98..507
                     /locus_tag="VBC74_002006"
                     /pseudo
     CDS             98..507
                     /locus_tag="VBC74_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            582..1262
                     /locus_tag="VBC74_002007"
     CDS             582..1262
                     /locus_tag="VBC74_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6942900.1"
                     /translation="MGDVVENFTEGKNPKIDTLNGKTVRIEKINPDHFEDLFQVYGEL
                     STEDSLTYMSFSKFNSKNEFDVFFQTLLKSEDPYYLAIVDNNTGKALGTFSLMRIDTK
                     NRVVEMGWVVYSSKLKQTRIATEAQYLVMKYVFEELCYRRYEWKCDSLNAPSNNSAKR
                     LGFTFEGTFRQAVVYKGRNRDTNWYSILDKEWPEKKTRFERWLDDSNFDVNEYQIRSL
                     SSIEQKTK"
BASE COUNT          537 a          160 c          257 g          448 t
ORIGIN      
        1 catagtacta atggaaaggt agcgacttca gcttgttgat ttatctaatg taaatgagtt
       61 tttagaattt tcatctgatt ttgaaactgt atttcatatg caacgatata aagccaatac
      121 cacagaagaa gcacattgat tttggcgaat gtctgtatga aatcattatt aggaatctat
      181 gcgatgattg aaacagaatc aaataaaatg attggtatta tagaattaga aattagagac
      241 gagtttccag cagaattcgg ttatatatta aataaaaatt atagtggaaa aggttatatg
      301 ataaaagctt gttcaaaatt gatgtcaatt ggatttgaac aactagattt ggaaagaatt
      361 tatgctcgat ttgaactaat aataaaaaat ctgaaaatgt tatggaatgc ctaagaatgc
      421 aaaaagaagg tgaacgtcgt catattgtaa aaaaatccaa aaggtgagtg gaaaaccaga
      481 gcatattatt ctattttaaa ggaataatat tttaaataga cgacaataaa aatttgtgaa
      541 agcagaaaaa ttaaatgaga tacaataggt ttaatcaact agtgggtgat gtcgttgaaa
      601 attttacgga aggtaaaaat ccgaagattg atacacttaa tgggaaaaca gttagaatag
      661 aaaaaataaa tccagatcat tttgaagact tatttcaagt ttatggagaa ttgtctactg
      721 aagatagttt aacttatatg tcttttagca aatttaatag taagaatgaa tttgatgtct
      781 ttttccaaac tctattaaaa tcagaagatc cttattattt agctattgtt gataataata
      841 ctggtaaagc attagggact ttttcattaa tgcgtattga tacaaaaaat agagttgttg
      901 aaatgggatg ggtggtttat tcatcaaaac taaaacaaac aaggatagca acagaagctc
      961 aatatttggt tatgaaatat gtttttgaag aactctgtta ccgccgttat gagtggaaat
     1021 gtgatagttt gaatgcacca tctaataatt ctgcaaaacg acttggtttt acttttgaag
     1081 gaacttttag acaagctgtt gtatataagg gtcgtaacag agatacaaat tggtattcta
     1141 ttttagataa agaatggcca gagaaaaaaa cacggtttga aagatggcta gatgattcaa
     1201 actttgatgt aaatgagtat caaatcagat cactatcaag cattgagcag aagacaaaat
     1261 agagtcattc attaatcact aaaatttcaa aattacatgt ttttttagct aacatcttaa
     1321 actttgttaa ataaatgttt attaactttt aaaggtgcat ggtaggactc aaaatcgggt
     1381 gtcctcacgg acgtgctagc tc
//
LOCUS       DATROD010000180         1398 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.183, whole genome shotgun sequence.
ACCESSION   DATROD010000180 DATROD010000000
VERSION     DATROD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.183"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(131..496)
                     /gene="rplL"
                     /locus_tag="VBC74_002008"
     CDS             complement(131..496)
                     /gene="rplL"
                     /locus_tag="VBC74_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEO6942901.1"
                     /translation="MALNIENIIAEIKEATILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGEAAAAKDSFDVELTAAGDKKVGVIKVVREITGEGLKEAKAIVDNAPSVIKEGASEA
                     EANEIKEKLEAAGASVTLK"
     gene            complement(560..1060)
                     /locus_tag="VBC74_002009"
     CDS             complement(560..1060)
                     /locus_tag="VBC74_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEO6942902.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVEQDTNLRR
                     SLRESDVEFKVIKNSILTRAAEKAGLEDLKELFVGPSAVAFSNEDVIAPAKVISDFAK
                     DAEALEIKGGSVDGKFTSVEEINALAKLPNKEGMLSMLLSVLQAPVRNIAYAVKAVAE
                     KDEEVA"
     misc_feature    complement(1090..1223)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
BASE COUNT          422 a          268 c          223 g          485 t
ORIGIN      
        1 aacaccccca caaaatattg tgggggtgtt tgtggggaag attcaattga atctttcctt
       61 ttttaatcgc cttaacttta acgaaaagac tttaaaacta ggctttcacc tttaaacaac
      121 caatagatga ttatttaaga gtaactgaag cacctgcagc ttcaagtttt tctttgattt
      181 cgttagcttc tgcttctgaa gcaccttctt tgataactga tggtgcgtta tcaacgatag
      241 ctttagcttc tttaagacct tcgcctgtga tttcacgaac aactttgata acgccaactt
      301 ttttgtctcc agcagctgtt aattcaacgt caaatgagtc tttagcagca gcagcttcac
      361 caccagcagc ggcagcagct acaggagcag cagcagttac accaaattct tcttcgatag
      421 cttttacaag atcgttaagt tcaagaattg tagcttcttt aatttcagca ataatatttt
      481 caatgtttaa tgccattgtt atttcctcca aaaatatgtt ttaatataaa attatgtagc
      541 taggctactt gaagagtgct tatgccactt cttcgtcttt ttctgcaact gctttgacag
      601 catatgcaat gttgcgtact ggcgcttgaa gtactgaaag gagcattgaa agcatacctt
      661 ctttgtttgg taatttagca agagcgttaa tttcttcaac tgaagtgaat ttaccgtcta
      721 ctgaaccacc tttgatttca agagcttctg catctttagc gaaatcgcta ataactttag
      781 ctggtgcaat aacatcttcg ttagaaaacg caactgctga tggaccaaca aaaagttctt
      841 taaggtcttc aagacctgct ttttcagctg cacgagttaa aattgagttt ttgataactt
      901 taaactcaac atcgctttca cgaagtgaac gacgtaagtt agtatcttgc tcaacagtaa
      961 gaccacgtga atcaacaaca acgatacttg ctgctgcttt cattttctca gcaataagtt
     1021 caacttgttc agcttttttt gcaataattg cttcactcat tagttttacc tccgttttta
     1081 ttttttggga taggcacaaa aaaatcgcac ctaaacctag acacgaaagt acaatacgtt
     1141 attttataat gacgttttgt gcctcggtag gaatattatg agcactgctc ccctactgtc
     1201 ttaggtcagt ttcatcagaa actttaaata gtataactta taattctagc attgtcaata
     1261 atttttatta tttttctgaa ataattttat agtaccaaaa aatactaaaa tagttatctt
     1321 tatatcaaaa aaataatatt ttagtattta tttattgacc tttactgact aaaggtgtat
     1381 tatagtacca gaaagata
//
LOCUS       DATROD010000181         1393 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.184, whole genome shotgun sequence.
ACCESSION   DATROD010000181 DATROD010000000
VERSION     DATROD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.184"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(170..1192)
                     /locus_tag="VBC74_002010"
     CDS             complement(170..1192)
                     /locus_tag="VBC74_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEO6942903.1"
                     /translation="MNIIIGTSLLILVLAIFTLFNYKAPYGTKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVTIAIGVTPGYAVLIGLSVSGTG
                     IIPGFLAGYLVGFLVKWMERNIPGGLDLISIIIIGAPLTRLVAKLLTPLINSTLLTIG
                     DILTSGAHSNPILMGVILGGTIVVVATAPLSSMALTAMLGLTGMPMAIGALSVFGSSF
                     MNGVLFHKLKLGSRKDNIAFAVEPLTQADVTSANPIPIYVTNFVGGAACGILIALMKL
                     VNDTPGTATPIAGFAVMFAYNPMIKVLITALGCIILSLLAGYFGGIVFKDYKLVTKEE
                     LQARDN"
BASE COUNT          500 a          271 c          221 g          401 t
ORIGIN      
        1 agttgataaa gaaatcttga aatttactga taccagcatt cttaacaata aaaaaattca
       61 acaatctttt tattagagtc gttgaatttt aaattatcaa taacagttat cacaagtcag
      121 atatcatatc tatattaatg atatttacta ttagcttttc atgaagtaac tagttatctc
      181 tagcctgtag ctcttctttt gtaactaatt tataatcttt aaaaacaatg cctccaaaat
      241 agcctgctag taaagatagg ataatacaac ctagagcggt tattagtact tttatcattg
      301 ggttataggc aaacatgaca gcaaatcctg caattggtgt cgctgttcca ggagtatcat
      361 taactaattt catcaaggca attaaaatac cacaagctgc accaccaaca aaattagtga
      421 catagattgg aatagggtta gctgaagtca cgtcagcttg agttagaggc tcaacagcaa
      481 aagctatatt atctttacga cttccaagtt ttaatttatg gaaaagtaca ccattcataa
      541 atgacgaacc aaagacagac aaggctccta tagccatagg cattccggtt aatcctagca
      601 tagctgtcaa tgccatagaa gaaagtggtg ctgtcgccac tactacaatt gtcccgccaa
      661 gtataacccc cattaaaata gggttgctat gagcacctga tgttaagata tctccaatgg
      721 tgagcaaagt gctattaata agcggcgtca acagtttggc cacaagacgt gttaatggtg
      781 caccaataat aataattgaa ataagatcta aaccaccagg aatatttctt tccatccact
      841 taacaagaaa tcccactaaa tatcctgcaa ggaaaccagg aataatacct gttccagaaa
      901 cagataaacc aattaaaact gcgtatcctg gggttacacc aatagcaata gtaaccaata
      961 tagctgcagc tacaccagaa agtgaaccat tagcaccacc cacttcactt aaaaattgaa
     1021 atcccaaaac tttaccaaag aaagaatctt gaaaagcttc tacaaggaag gatgcacaag
     1081 ccgcagatgc caatgctccc attgcttttg ttccgtaggg tgctttataa ttaaataaag
     1141 tgaaaatcgc taatacaagt attaaaagac tggtcccaat aatgatattc ataaatatac
     1201 tcccgctaaa aatagttaag ttttttattc tcagtgtctg tgtactactg cttcaatttc
     1261 aataaaggcg cctttaggta atactgaaac ttaattgtac tacgaactgg ataaggtgca
     1321 acaaagaagt ccgacataat tattaggctt atctcatgga ttactcctta gttgctaata
     1381 taatcacatt tta
//
LOCUS       DATROD010000182         1325 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.185, whole genome shotgun sequence.
ACCESSION   DATROD010000182 DATROD010000000
VERSION     DATROD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.185"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            11..1198
                     /locus_tag="VBC74_002011"
     CDS             11..1198
                     /locus_tag="VBC74_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEO6942904.1"
                     /translation="MPTISAESFNASAKHALAVDLDSGKILYEKDANKPAAIASLTKI
                     MTVYMVYKEIDNGNLKWNTKVNISDYPYQLTRESDASNVPLEKRRYTVKQLVDAAMIS
                     SANSAAIALAEHISGTESKFVDKMTAQLEKWGIHDSHLVNASGLNNSMLGNHIYPKSS
                     QNDENKMSARDIAIAAYHLVNEYPSILKITSKSVAKFDKDIMHSYNYMLPDMPVFRPG
                     ITGLKTGTTELAGQSFIATSTESGMRLLTVIMHADKADKDKYARFTATNSLLNYITNT
                     YEPNLVLAKGAAYKGKEASVRDGKEQSVIAVAKNDLKVVQKKNITKQNQLKINFKKEL
                     TAPITKKENLGKAYYVDLNKVGKGYLIKEPSVHLVAKDSIERSFFLKVWWNHFVRYVN
                     EKL"
BASE COUNT          459 a          234 c          233 g          399 t
ORIGIN      
        1 catttttttt atgccaacca tctctgctga atcttttaat gcttccgcta aacatgcctt
       61 agcagttgat ttagattcag gaaaaatctt gtatgaaaaa gatgctaaca aacccgctgc
      121 tattgcttcc ttgactaaaa taatgaccgt ttatatggtc tataaagaaa ttgataacgg
      181 taacctcaag tggaatacca aagtaaatat atctgactac ccttatcaac taacacgcga
      241 atctgatgct agtaatgttc ctttagaaaa aaggcgctat actgttaaac aactcgtgga
      301 cgctgccatg atttctagtg ctaacagtgc agccattgct ttagctgaac atatttcagg
      361 aactgaaagt aaatttgttg ataaaatgac tgctcaattg gaaaagtggg gaattcatga
      421 tagccaccta gtcaatgctt ctggcttaaa taatagtatg ttaggcaatc acatttatcc
      481 aaaatcgtca caaaacgacg aaaataaaat gagtgcacgt gatattgcta ttgctgccta
      541 ccatttggtc aacgaatatc cttccattct taagattact agtaagtccg ttgctaaatt
      601 tgataaagat attatgcatt cttataacta catgctacca gatatgcctg tctttagacc
      661 aggtattaca ggtttgaaaa ctgggacaac ggaattagct ggccaatctt ttattgctac
      721 atctactgaa agtggaatga gactactcac tgttattatg catgctgata aggccgataa
      781 agacaaatat gctcgcttta cagcaactaa ctctctcttg aactatatca caaacaccta
      841 cgaacctaac cttgtattag ctaaaggagc tgcatataaa ggtaaagaag caagtgtgag
      901 agacggaaaa gaacaatcgg tcatcgctgt tgctaaaaac gatttgaaag tagtacagaa
      961 gaaaaatatc actaaacaaa atcagttaaa aattaacttt aaaaaagagc ttactgctcc
     1021 tattacaaaa aaagagaacc tagggaaagc ttattacgtt gaccttaata aggttggaaa
     1081 aggctatctc ataaaggaac ctagcgttca tttagtggca aaagatagta ttgagcgcag
     1141 tttcttcctc aaagtgtggt ggaatcattt tgtgcgctac gttaacgaaa aactttaata
     1201 gtaatctccc tttgacacaa ggagttactg cacagcagtt tctttagcta ggtttaggag
     1261 aatatataaa attaaaaatc actaatgaaa tgtctttttt attagtgatt ttttgttata
     1321 atatt
//
LOCUS       DATROD010000183         1286 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.186, whole genome shotgun sequence.
ACCESSION   DATROD010000183 DATROD010000000
VERSION     DATROD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.186"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..452)
                     /locus_tag="VBC74_002012"
     CDS             complement(<1..452)
                     /locus_tag="VBC74_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEO6942905.1"
                     /translation="MKNFIKQSDTTFVRIIKSLLIGGFIGAILGSVGALFNIFGQDKY
                     LSEINIVQYFLWVSRIVVIITALFSLIYLYQIQKYQKVFFNVDESQSEEIYRQINLRH
                     SYGMTFVSISIVLSIFNTLFNYKLNIFDDSVTLVIPIYDLSLLFVLLG"
     gene            complement(455..670)
                     /locus_tag="VBC74_002013"
     CDS             complement(455..670)
                     /locus_tag="VBC74_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6942906.1"
                     /translation="MGLILKNRLKELRARDGINQTELAKLTGVSRQTISLIERGEYTP
                     SVIIAMKISQVFKESLENVFSLVSTEE"
     gene            complement(715..1008)
                     /locus_tag="VBC74_002014"
     CDS             complement(715..1008)
                     /locus_tag="VBC74_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942907.1"
                     /translation="MVMTYIGVPKEQKALSRRGKQLEKINQGGKTLMSQMIQDLVGST
                     CMIYLEDGFQTHPYEILKVDEDWVQIRRNLKRSKEEVRILRIHDIKELRPVNK"
     gene            complement(1040..1228)
                     /locus_tag="VBC74_002015"
     CDS             complement(1040..1228)
                     /locus_tag="VBC74_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942908.1"
                     /translation="MKKEFIITGRMVARVMETLVCLCMVSNLVLDMLDIIEINEIVFC
                     VLVASIAGLEFCLQKERE"
BASE COUNT          443 a          248 c          156 g          439 t
ORIGIN      
        1 aaacccaata aaacaaataa cagtgataaa tcatatattg gaataactaa agtaactgaa
       61 tcatcaaaaa tatttagttt atagttaaat aaagtattaa atatactgag tactattgaa
      121 attgacacaa aagtcatccc ataagaatgt cttaaattaa tctggcgata aatttcttca
      181 gattgacttt catcaacatt gaaaaaaact ttctgatatt tttgaatctg atagaggtag
      241 atgagactaa agagagctgt tataattaca acaatacgac taacccatag gaaatattga
      301 acaatattaa tttcagataa gtacttatct tgaccaaata tgttaaaaag agctcctacg
      361 gaaccaagta tggcccctat aaaaccacca attaaaagac ttttgataat tcgaacaaat
      421 gtagtatcac tttgttttat aaaatttttc atcgttattc ctccgttgac actaaactaa
      481 aaacattctc tagggattct ttaaatactt gagaaatctt catagcaata ataacagatg
      541 gcgtatattc tccgcgttca attaggctaa ttgtttgtct tgagactccc gttaatttgg
      601 cgagttctgt ttgattgata ccatctcgtg cacgcaattc tttgagcctg ttttttaaaa
      661 ttaatcccat catttttctc ctttataaaa tgatatatag atttattcct cactctattt
      721 attgactgga cgcaattcct tgatatcatg aatacggaga atacgtacct cctctttact
      781 tcgtttcaag ttgcgacgaa tctgtaccca atcctcatct acctttaaaa tctcataagg
      841 atgcgtttga aaaccatcct ctaaataaat catacatgtg cttcctacta aatcttgaat
      901 catttgtgac ataagtgttt ttcctccctg atttattttt tctaattgtt tacccctacg
      961 actgagtgcc ttttgctctt taggtacacc aatatacgtc atgaccataa aaagccatta
     1021 agccaaaaat tccatctatc tactcccttt ctttttgcaa acaaaactca agccctgcaa
     1081 ttgatgctac caacacacaa aatacaattt catttatttc tattatatca agcatatcaa
     1141 gaacaagatt acttaccata cataagcaaa ctagggtttc cataactctt gcaaccatcc
     1201 gacctgtaat aataaattcc tttttcattt gagtaactcc taaaaccatt atgcttttga
     1261 aacaatgatt aatttgattg ttcaat
//
LOCUS       DATROD010000184         1246 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.187, whole genome shotgun sequence.
ACCESSION   DATROD010000184 DATROD010000000
VERSION     DATROD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.187"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..100
                     /locus_tag="VBC74_002016"
     CDS             <1..100
                     /locus_tag="VBC74_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEO6942909.1"
                     /translation="KVASVIGYKNKKMVWGHITREKQMKDFETSVT"
     gene            340..840
                     /locus_tag="VBC74_002017"
     CDS             340..840
                     /locus_tag="VBC74_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEO6942910.1"
                     /translation="MACIIRRARLGDEVNLAYIQTESWKAALGKILPEDIIQKTTEIE
                     PAITMYQQLLHKEVGKGYILEVDSNPHCMAWWDKSREDGMLDYAELICIHSLKEGWGK
                     GYGSQMMNHVLSEIQQAGYNKVILWVFTENTRARKFYDRFGFSFKGKSKTYFEKEEMC
                     YEKILH"
     gene            1038..>1246
                     /locus_tag="VBC74_002018"
     CDS             1038..>1246
                     /locus_tag="VBC74_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942911.1"
                     /translation="MEKITNDFEKVIAVLNQLSISYELVENGPVLLTEQADNFIVGIE
                     GVRTKIMFLTNKKKTKYYLLIMDDQ"
BASE COUNT          484 a          133 c          255 g          374 t
ORIGIN      
        1 taaggttgca agtgttattg gctataaaaa taaaaaaatg gtttgggggc atattacgag
       61 agagaaacaa atgaaggatt ttgaaacaag tgttacttag aagttatagc gattcatata
      121 tataaaataa tcactaaaag agcgtaatta tctacgctct tttagtgatt atcaataatt
      181 ggcctaatct gaggttaatg tttaaaacca tctaaaattt tagaaatata atgataaatg
      241 agaaccagaa ctgttcaaaa taaaaattaa tatcacgaaa atggtataat tggagtagtt
      301 atcaggcagt taataataat ttttgaaagg attttagtta tggcttgcat aatcaggaga
      361 gcaagattag gagatgaagt caatttagct tatattcaaa cggagagctg gaaagctgct
      421 ttgggtaaga tactacctga ggatatcatt caaaaaacta cggaaataga acctgccata
      481 acaatgtatc aacagctact acacaaggag gttggtaagg ggtatatatt ggaagtagat
      541 agcaatcctc attgtatggc ttggtgggat aaaagtagag aagatggtat gcttgattat
      601 gcagaactca tttgtataca tagtcttaaa gaaggttggg gaaaaggtta tgggtctcaa
      661 atgatgaatc atgtactatc agagatacaa caagcaggtt ataataaagt gatactttgg
      721 gtatttacag aaaatacacg cgcaaggaaa ttttacgata ggtttggttt ttcttttaag
      781 ggtaaatcaa aaacttattt tgaaaaggaa gaaatgtgtt atgagaagat tttgcactag
      841 cgagataaag catccgataa tacaatagta taaacatcct attttgaaaa acaatttcct
      901 ttaaggggaa caaatttgta aaagaaaatt gttataggta taatgaagat tatgaaggta
      961 aaattttttg agtgaaaaac agtgtatcaa ttttaggttc tctagtctac aaatagttat
     1021 gctaactcta tggaagaatg gagaaaatta cgaatgattt tgaaaaagtt atagctgtgc
     1081 taaatcaact aagtatttct tatgaattag tggaaaatgg acctgttttg ttgacggagc
     1141 aagcagataa ctttattgtg ggaattgaag gagtaagaac aaaaataatg ttcttgacca
     1201 acaagaaaaa gacgaaatat tatttactaa taatggatga ccagaa
//
LOCUS       DATROD010000185         1201 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.188, whole genome shotgun sequence.
ACCESSION   DATROD010000185 DATROD010000000
VERSION     DATROD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.188"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            145..1201
                     /locus_tag="VBC74_002019"
                     /pseudo
     CDS             145..1201
                     /locus_tag="VBC74_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS861 family transposase"
BASE COUNT          389 a          218 c          277 g          317 t
ORIGIN      
        1 gacaaaaaat ctaacgattg gggtgctttt ctgatgaaat taagttatga agataaacta
       61 gaaatatacg agttaagaaa gatcgggatg tcctggtctc agattagtca acgatatgat
      121 gttcgtatct cgaatcttaa atacatgata aaactcatgg atcggtatgg tgtagaaatc
      181 gtcgaaaaag gtagaaatga gtattatcca cctgaattaa agcaggaaat gatagataaa
      241 gtcttgattc atggttgttc tcaactctct gtttctcttg attatgctct ctctaattgt
      301 tctatactca caaattggct ttcccaattt aagaaaaatg gttatactat tgttgagaaa
      361 acaagaggga gaccgagtaa aatgggacgt aagcgtaaga aaacttggga agaaatgaca
      421 gagctcgagc gacttcagga agaaaatgag cgcttgagaa cggagaatgc ctttctaaaa
      481 aagttgaggg atttgcgctt gagggacgaa gccttacaga gcgaacggca gaaacaatta
      541 gagaaatggt cacaggagga ttccgactag acctcttact tgagataact aagatagctc
      601 gtgcaactta ctattatcaa ctaaagaaac tgaataaacc agataaagac aaagcaatca
      661 aatctgacat tcaatccatt tatgatgaac atagaggaaa ctatggctat cgtcggattt
      721 atttagaact gcgaaatcgt ggctttgtca tcaaccacaa aagggtgcaa ggcttgatga
      781 aaagtatggg cttgactgct cgtattcgtc gtaagcgcaa gtatgcctct tacaaaggtg
      841 aggttggtaa gaaggctgat aacctgattc aacgccagtt tgaggggtcg aagccctatg
      901 agaagtgcta taccgatgtg acggagtttg ctttacctga agggaaactt tatctatcgc
      961 ctgttcttga tggctataac agtgagataa ttgattttac cctgtctcgg tctcctgcct
     1021 tgaaacaggt tcaaaccatg cttgagaggg cttttcctgc ggcttcatac agcgaaacca
     1081 ttctccacag cgaccaaggc tggcagtacc agcacaagtc ctaccatcaa tttctggagg
     1141 acaaaggcat tcgtccttct atgtcacgga aggggaacag tcctgacaac ggtatgatgg
     1201 a
//
LOCUS       DATROD010000186         1183 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.189, whole genome shotgun sequence.
ACCESSION   DATROD010000186 DATROD010000000
VERSION     DATROD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.189"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..77
                     /locus_tag="VBC74_002020"
     CDS             <1..77
                     /locus_tag="VBC74_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HEO6942912.1"
                     /translation="NNSKEELFNRKVIEKIIRTSIENY"
     gene            87..1073
                     /gene="dhaQ"
                     /locus_tag="VBC74_002021"
     CDS             87..1073
                     /gene="dhaQ"
                     /locus_tag="VBC74_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DhaKLM operon coactivator DhaQ"
                     /protein_id="HEO6942913.1"
                     /translation="MRKTEHHKMEYVFETIKAITKTNPKVEQIKDLPAIHYLGQSEQV
                     IPILSGGGSGHEPAHFGYVGEGMLSAAISGPIFVPPCASDILETIRFINRGKGVFVII
                     KNFEADLEEFSQAIEQARQEGIPIKYIVSHDDISVETSNFKIRHRGVAGTVLLHKIIG
                     QAALEGASLDELEQLGLSLTTSMATLGVASKSATILGQHQPVFDLEEGYISFGIGIHG
                     EPGYRTMPFVSMEHLANELVNKLKMKLRWQDGEAFILLINNLGGSSKMEELLFTNAVM
                     EFLALDDLQLPFIKTGHLITSLDMAGLSVTLCRVKDSRWIDYLKHKTDARAW"
BASE COUNT          409 a          172 c          246 g          356 t
ORIGIN      
        1 taaataactc aaaagaagaa ttatttaata ggaaagtcat tgaaaaaatt atcagaacat
       61 caatagaaaa ttattaggac aaggatatga gaaaaacaga acatcataaa atggaatatg
      121 tttttgaaac aataaaagca atcactaaaa ctaatcctaa agtagagcaa ataaaagatt
      181 tgcccgctat tcattattta gggcaatccg agcaagtaat tccgatttta tcaggaggag
      241 gcagcggaca tgagccggct cactttggat atgttggtga gggaatgcta tcggcagcga
      301 ttagtggtcc tatttttgta ccaccatgtg ctagtgatat tctagagact attagattta
      361 tcaatcgagg caaaggtgtc tttgttatta tcaaaaattt tgaagctgat ttggaagaat
      421 tttcccaagc gattgaacag gctcgtcaag aaggcattcc aatcaaatac atcgtttctc
      481 atgatgacat ctctgttgaa acatcaaatt ttaaaattcg tcatcgaggc gttgctggta
      541 ctgtgttgct acataaaatt attggacaag ctgctttaga aggagcaagc ttagatgaat
      601 tagaacagct aggactatct ttgacaactt caatggctac tttaggtgta gcaagtaagt
      661 cagccactat tttaggtcag catcaacctg tttttgattt agaagaaggt tacatctcat
      721 ttggaatcgg tattcatggt gagccaggat accggacaat gcctttcgtg tccatggaac
      781 atttggcaaa cgaactagtt aacaaattga aaatgaaatt acgttggcaa gatggagaag
      841 cattcattct attgataaat aatcttggag gaagttctaa aatggaggaa ttacttttta
      901 caaatgctgt catggaattt ctggctttag acgatttaca attacctttt attaaaacag
      961 gccacctaat tacaagttta gatatggctg gtttatcagt taccttgtgt cgtgtaaagg
     1021 atagtagatg gattgattac ttaaaacata agacagatgc tagagcttgg tgatagaagt
     1081 aagtggtaag agtttttaga atagaaaata ctttaaggag aacagagaaa tctgttctct
     1141 ttttgttcgc ttaaagatca tagaaaggtg aagagaacat gga
//
LOCUS       DATROD010000187         1181 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.190, whole genome shotgun sequence.
ACCESSION   DATROD010000187 DATROD010000000
VERSION     DATROD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.190"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..196)
                     /locus_tag="VBC74_002022"
     CDS             complement(<1..196)
                     /locus_tag="VBC74_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 family protein"
                     /protein_id="HEO6942914.1"
                     /translation="MIYKIIASLFLVLIPIFSQVLVKIFKLKKFNIMFPDVAFPIFVF
                     LIPLISSSLLKQNLLPYYLIL"
     gene            complement(257..1162)
                     /locus_tag="VBC74_002023"
     CDS             complement(257..1162)
                     /locus_tag="VBC74_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6942915.1"
                     /translation="MKNQQKLMYLESIELYSNITKAAAHLFISQPYLSKVIKQLENEL
                     EIKLIQSQGHQTFLTYAGQRYLFYLKEIDMIERQMAKELYLIRSDKKGEITLGINSGL
                     ASSILANVLPKFNLEHPEISVKLLENNQNISEQLVASGDIDLAVGMAPILYKDGIAST
                     TIYRDELFLMIPTTSQLYNAEKRGQIIPFEYPISVLDNEPLILTPLEYGIGKTIAQFY
                     ELHHMSLNQMITTSTVPTAASLSLSGMGATFVPQTLIHRYLDKECNVYHFHKNKLFSE
                     YIMIYKKDVELSGIALLLYKAFLTK"
BASE COUNT          393 a          164 c          226 g          398 t
ORIGIN      
        1 tgagaatcag atagtaaggt aaaagatttt gttttaacag actagatgat attaatggaa
       61 ttaaaaaaac aaaaatagga aaagcaacat ctggaaacat aatgttaaat tttttaagtt
      121 taaatatttt gacaagaacc tgtgaaaata tagggattaa aacaagaaat aaagaagcaa
      181 taattttgta tatcataggt ctattttata ccgattttgg taagaagtaa actgattttc
      241 aatagaatta gcgaaactac ttggttaaaa aagctttata aagtaggaga gcaattccag
      301 ataattcaac atctttttta taaatcataa tgtactctga aaataactta ttcttatgaa
      361 aatggtagac attgcactct ttatccaaat agcgatgaat gagagtttgt ggaacaaatg
      421 ttgctcccat ccctgacaaa gataggctag ctgctgtagg aacggtacta gttgtaatca
      481 tctgatttaa tgacatgtga tgaagctcat agaattgtgc aattgttttg ccaattccgt
      541 actctaatgg tgttaggata agaggttcat tgtctagaac tgaaatagga tattcaaaag
      601 gaataatctg tccacgtttt tcagcgttgt agagttggct ggtggtaggt atcattaaaa
      661 acaattcgtc gcggtaaata gtggttgatg caattccatc tttataaagg ataggagcca
      721 tcccaacagc taggtcaata tcaccactcg ctaccaattg ttcagaaata ttttgattgt
      781 tttcgagtaa tttgacagaa atttctgggt gctccaagtt aaatttggga agtacatttg
      841 ccagaataga acttgctaag ccagagttaa tcccaagtgt gatttctcct tttttatcag
      901 aacggatgag atatagttct ttggccattt gacgctcaat catatcaatt tcttttaggt
      961 agaatagata tctctgtcca gcataagtta aaaaggtttg gtgtccttgg ctctggatga
     1021 gttttatttc taactcattt tctaattgct tgatcacttt gcttaggtaa ggttgagaaa
     1081 taaaaaggtg tgcagcggct ttagtgatgt ttgagtatag ctcgatactc tctagataca
     1141 ttaatttttg ttgatttttc aagactttat aaccagtttg a
//
LOCUS       DATROD010000188         1172 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.192, whole genome shotgun sequence.
ACCESSION   DATROD010000188 DATROD010000000
VERSION     DATROD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.192"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            245..1168
                     /locus_tag="VBC74_002024"
     CDS             245..1168
                     /locus_tag="VBC74_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEO6942916.1"
                     /translation="MRIQQLQYVIKIVETGSMNEAAKQLYITQPSLSNAVRNLETEMG
                     IQIFIRNPKGITLTKDGMEFLSYARQILEQTALLEERYKGDNTSRELFSVSSQHYAFV
                     VNAFVALFNGTDMTQYELFLRETRTWEIIDDVKNFRSEIGVLFLNSYNRDVLTKLFDD
                     NSLIATTLFTTTPHIFVSKSNPLANRKKLSMKDLEDYPYLSYDQGLHNSFYFSEEMMS
                     QIPHPKSIVVSDRATLFNLMIGLDGYTVATGILNSKLNGDEIVAIPLDVDDVIDIVYI
                     RHDKANLSKMGQKFIDYLLEEVSFNHKNKQS"
BASE COUNT          394 a          191 c          192 g          395 t
ORIGIN      
        1 cccgtagagg tgtatgtgag tgtttctttt ggtgacggcg cgaatgatta gacattgtaa
       61 ttctccaata cttaaaaaaa ccttaatttt acttggatat tatatcattt ttcaatacaa
      121 tattcaacga aaataatatt ggaataatct gattatatta gctaaataac attttcaatt
      181 gatctatatt gataatttag ctgatctaat tgaatttatt tacattctag tttaaactag
      241 aattatgaga attcaacaat tacaatatgt tattaagatt gtagaaactg gtagcatgaa
      301 tgaagccgct aaacaactct acattaccca accttcactt tcaaacgctg ttaggaacct
      361 tgagactgag atgggtatcc aaatttttat tcgaaatcca aaaggaataa cgttaacaaa
      421 agatggtatg gaatttctgt catatgcgcg acaaattcta gaacaaacag cacttcttga
      481 agagcgttat aagggtgata atacaagtcg tgagctgttc agcgtttctt cacaacacta
      541 tgcatttgtg gttaacgctt ttgtagctct ctttaatggc acagacatga cccaatacga
      601 acttttttta cgtgaaacta ggacttggga aattattgat gatgttaaaa atttccgttc
      661 agagattggt gttctgtttc ttaatagcta taatagagat gtattaacca aattattcga
      721 cgataattcc ttaattgcca caactctttt tactactaca cctcatattt tcgtgagcaa
      781 atccaatccc ttagctaata gaaaaaaatt aagtatgaaa gatttagaag attatcctta
      841 tctgagttat gatcaaggcc tgcataattc tttctacttt tctgaggaaa tgatgtctca
      901 aattcctcac ccaaaatcca ttgttgtcag tgatagagca actctgttta atttaatgat
      961 tggattagat ggttatacag tggcaactgg tatcctcaac tcaaaattga acggtgacga
     1021 aattgtcgca atccctcttg acgtggatga tgttattgat attgtctata tccgacacga
     1081 caaagcaaac ctttcaaaaa tgggacaaaa atttattgac tatttattag aggaagtttc
     1141 gtttaatcat aaaaataaac agtcctgaga tt
//
LOCUS       DATROD010000189         1169 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.193, whole genome shotgun sequence.
ACCESSION   DATROD010000189 DATROD010000000
VERSION     DATROD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.193"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(115..369)
                     /locus_tag="VBC74_002025"
     CDS             complement(115..369)
                     /locus_tag="VBC74_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001923489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEO6942917.1"
                     /translation="MFVMRTFGNSLSGPLVVILSSILFSWSHLHGLSVVDFVVYFGMG
                     LIFASLHHYTKSIHYSIGEHIVWNSLSYIFYFLAFLLDLL"
     gene            complement(388..639)
                     /locus_tag="VBC74_002026"
     CDS             complement(388..639)
                     /locus_tag="VBC74_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942918.1"
                     /translation="MMILLSYVLTKTEPISNYQESSSNLKKWLFAIGAFSVLFCWALL
                     VKTFFPIRQNQEGLIADMNQVNHYFRVVLLLTICLIAPI"
BASE COUNT          450 a          201 c          177 g          341 t
ORIGIN      
        1 aacaataaaa taataacatt ttattcatta aaaaaacgaa taacaaaaga gccaaaaagc
       61 tcttttaaac tgacaaaaat gtcttagtaa tgattagtct ctcaatctta ttacttagag
      121 taaatcaagc aaaaatgcta gaaaataaaa aatatatgac aaactattcc aaacaatatg
      181 ctcgccaatc gaataatgaa tggattttgt atagtggtgc aaactagcga aaatcaaacc
      241 cataccaaaa tagacaacaa agtcgacgac agatagacca tgtaagtgag accagctaaa
      301 tagtatagaa gataagatca caaccaaggg accactcagg gagttcccaa atgttctcat
      361 gacaaacaac ctgaacgtta tctcttctta aattggggca ataagacaga tagtcaataa
      421 taagacaact cgaaagtagt gattgacctg attcatatca gctattaatc cctcctggtt
      481 ttgccttatt gggaaaaatg tttttactag tagtgcccag caaaataaaa cgctaaaagc
      541 acctattgca aatagccatt ttttgaggtt agatgatgac tcttggtagt tactgattgg
      601 ctcagttttt gttagcacat aactcaacag aatcatcatt ataagattaa tgccaatcca
      661 tatactccaa ctccactcta accctattga tagtaaacta atcacccaat gcccaagcaa
      721 ttgttattat tgtgttgttg caactttggc aaagatgaat atagcaggat ggaaaacaga
      781 ttgtttctga aacttcaaag gtatcttctt atcacaagct attatttggt tatctccata
      841 attaatctaa atggagcaat atatcgtttt tctactaact gttgacaaat aaaaaactcc
      901 cctttaaagt ctggtaactt ttcgattttt accagaaact taaggagagt gaggcaagaa
      961 ctaatagaat cttatttaaa gttttatgat tgggagaaca gaacaactat cataattact
     1021 gaaaaattat gaaaggcatg gcaaacaaca gatggtcaaa tccgctctgt tgaaaacaaa
     1081 ttttaaatat aattcatttc actttctaag tttattaaac atagagtgtt caggagaaat
     1141 gatacgacct aatgataaaa tcattccta
//
LOCUS       DATROD010000190         1120 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.194, whole genome shotgun sequence.
ACCESSION   DATROD010000190 DATROD010000000
VERSION     DATROD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.194"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..514
                     /locus_tag="VBC74_002027"
     CDS             <1..514
                     /locus_tag="VBC74_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEO6942919.1"
                     /translation="LISLSGEKRSYLERRTPINRRTELEYYMPNFIYKDHLLPLDDDR
                     YFQMTLWVNENLVSEMLLDISEHFKNHHIRLTSSGFGCIDVLPADVNKADGIAILLEK
                     WGLKQDQVMVFGDGGNDVEMLRAANISYAMSNAPEEIKAIAKYQTVSNDQDGVLETIE
                     NFLHKMSDNE"
     gene            615..>1120
                     /locus_tag="VBC74_002028"
     CDS             615..>1120
                     /locus_tag="VBC74_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEO6942920.1"
                     /translation="MLEVIDKALRDYQMKREQFFEINNQTVQEGAIVFTGDSIVEFFP
                     LKKHLGRDYPLVNRGVAGSDSYWLLENLRTQVWELLPSKVFILIGTNDIGLGHSQSEI
                     IANITDIIAEIRAESYMTEINILSVLPVSEEDDYIERVKVRNNQAIKALNKTLSVISG
                     INYIELYD"
BASE COUNT          390 a          154 c          224 g          352 t
ORIGIN      
        1 actcatcagc ttatctggtg aaaaacgttc ataccttgag cgaagaactc ctattaatag
       61 gcggactgaa ttagaatact atatgcctaa ttttatctat aaagaccatt tgctgccctt
      121 agatgatgac cgatattttc aaatgacact ttgggtaaat gaaaatcttg tttctgagat
      181 gttgttagat attagtgaac actttaaaaa tcatcatatc agattgacct ctagtggttt
      241 tggttgtatc gatgtcttac cagctgatgt caataaagcg gatggtatcg ctattctcct
      301 tgaaaagtgg ggattgaaac aggatcaagt gatggttttt ggtgatgggg gcaatgatgt
      361 ggaaatgttg agagcagcaa atatttctta cgctatgtca aatgcgccag aagaaattaa
      421 agctattgct aaatatcaaa cagttagtaa cgatcaagat ggtgtgttag aaacgataga
      481 gaactttctt cacaagatga gtgataatga atagaaccac gtagcttttc acgaagcata
      541 ctattcaatt ctcaggtgaa attttgttat aatacaagaa agcctagaga aatatttaag
      601 aatgaaggag aacgatgtta gaagttattg acaaagcttt aagagattat caaatgaaac
      661 gagaacagtt ttttgagatt aacaatcaga cagtacaaga aggtgccatt gttttcacag
      721 gagattctat cgttgagttt tttcctttaa aaaaacattt aggacgtgac tatcctttag
      781 ttaatagagg agtagcagga tcagattctt attggctttt agaaaacttg agaacccaag
      841 tatgggaact attaccatcc aaagtattta ttttaattgg aacaaatgat attggtttag
      901 ggcattcaca gtcagaaatc atagctaata tcacggatat aattgcagaa attcgtgcgg
      961 aatcttatat gacagagatt aatatactga gtgttttacc tgttagtgaa gaagatgact
     1021 atatagagcg tgtcaaggta agaaataatc aagcaataaa agctttaaac aaaaccttat
     1081 cagtaatttc tggcattaat tatattgaat tgtatgattt
//
LOCUS       DATROD010000191         1108 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.195, whole genome shotgun sequence.
ACCESSION   DATROD010000191 DATROD010000000
VERSION     DATROD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.195"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            8..496
                     /locus_tag="VBC74_002029"
     CDS             8..496
                     /locus_tag="VBC74_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEO6942921.1"
                     /translation="MITLLKDQSFDEISTINLTKTAGISRSSFYTHYKDKYEMIDQYQ
                     QSLFNKVEYIFDRNQFKKEDALLEIFQFLDRESLFAALLTQNGTKEIQTYILNKLQLM
                     LSKELPVVNPDATKSDINRLYYSVYLSHAIFGVYQMWITRGKKESPQQITQVLLSLLP
                     QT"
     gene            complement(582..878)
                     /locus_tag="VBC74_002030"
     CDS             complement(582..878)
                     /locus_tag="VBC74_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEO6942922.1"
                     /translation="MNLNDRLKIEEMEEKYDSFKPRINALVEAIDDFQKHYEDYVKLR
                     EFYGSEDWFRLSEQTENNLKCGVLSEDQLFDFIGEHNELVGQFLDMSSQMYRHL"
BASE COUNT          389 a          192 c          141 g          386 t
ORIGIN      
        1 agcagctatg attacgttat taaaagatca gagcttcgat gaaatttcaa cgattaatct
       61 aactaaaaca gctggaatca gtcgttctag tttttatact cattataaag ataaatatga
      121 aatgattgat caataccaac agtcactctt caataaagtc gaatatattt ttgatcgcaa
      181 tcaatttaaa aaagaagatg ccttgctcga aatttttcaa ttcttagata gagaatcatt
      241 atttgctgct ctcttaaccc aaaatggaac aaaagaaatt cagacctaca tccttaataa
      301 attacaatta atgctttcta aagaattacc tgtggtcaat ccagatgcaa caaagtctga
      361 tatcaaccgt ttgtattata gtgtctatct cagtcatgct atttttggag tctatcaaat
      421 gtggataaca cgaggaaaaa aagaaagccc tcaacaaatt acacaagtgc tactcagttt
      481 attacctcaa acttaaaacc agccttcctt ataggctggt catttttagt taggtttatt
      541 ttatcctcat tataggataa caacactaat aaggactgac attataaatg acgatacatt
      601 tgagaagaca tatctaaaaa ttggcctaca agctcgttat gttctcctat aaaatcaaag
      661 agctgatcct cactcagtac accgcatttg agattatttt ctgtttgttc tgatagtcta
      721 aaccagtctt cgctaccata aaattctctt aatttcacgt aatcttcata gtgcttttga
      781 aaatcatcta tagcttctac taatgcattg attctaggtt tgaagctatc atacttctct
      841 tccatttctt ctatttttaa tctatcattt agattcataa taaaatctcc ttagttatat
      901 aagattcgac gagataagtt atcattctca agtatttgta acgttaactc tactatatcc
      961 tttttacaaa gtaaaaaatt actcattaca aactgacagt gttgattttt attctaatgt
     1021 tagtttaaaa tctttttggt tcattaaata taatgactca ctaaaaagta tatagcaaaa
     1081 aaagcctata aaataggctt ttaaagta
//
LOCUS       DATROD010000192         1070 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.196, whole genome shotgun sequence.
ACCESSION   DATROD010000192 DATROD010000000
VERSION     DATROD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.196"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..296
                     /locus_tag="VBC74_002031"
     CDS             <1..296
                     /locus_tag="VBC74_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942923.1"
                     /translation="KQEQDRIVNYIKQHVELTNGNQIKKIEFIDFQKNEMTGTWGIST
                     KINEQFSISFSEDRIGGKLRALGYQPNEIGFSKDINSNNQNVNDIEVIYMKKE"
     gene            298..807
                     /locus_tag="VBC74_002032"
     CDS             298..807
                     /locus_tag="VBC74_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001869186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942924.1"
                     /translation="MISEESYRYLVEDAYRVDSKKVKIPLKRSDIVGNSDYVIIEPPI
                     DNTSNGMQAMVVAPIKEGMTAEPDTSEIVIVYAGTNLGERLDIATDVEMVAGGDTYLL
                     ADPKTKTFRKSQGKSALEYAEKISSKYPNSEITTTGHSLGESEALYVALKMGWMNVGY
                     NGPDIHNMI"
BASE COUNT          426 a          115 c          209 g          320 t
ORIGIN      
        1 ggaaacagga acaagataga attgtcaatt acataaaaca acatgttgag ttaacaaatg
       61 gtaatcaaat aaaaaaaatt gagtttatcg actttcaaaa aaatgagatg acaggtacat
      121 ggggaatttc tactaaaatt aatgaacaat tttcgattag tttttctgaa gatagaattg
      181 gtggtaaact tagagcatta ggatatcaac cgaatgaaat aggtttttca aaggacatca
      241 atagtaataa tcaaaatgtt aatgatattg aagtgattta tatgaagaaa gaatagaatg
      301 atttcagaag aaagttacag atatttagtt gaggatgctt atcgagttga ttcaaaaaaa
      361 gtaaaaatac ctctgaaaag aagtgatatt gtagggaaca gtgactatgt tattattgaa
      421 ccacctatag ataatacttc aaatggtatg caagctatgg tagttgctcc gataaaagaa
      481 ggaatgacag ctgaaccaga tactagtgaa atagtgattg tttatgcagg tactaattta
      541 ggtgagcgtt tagacatagc tacagatgtt gaaatggttg ctggagggga tacatattta
      601 cttgctgatc ctaaaaccaa gacttttagg aaatctcagg gaaaatcagc actagagtac
      661 gcagaaaaaa tatcgagtaa atatcctaat tcagaaatta ctacaaccgg tcactcctta
      721 ggggaatcag aggcactata tgtcgcttta aaaatgggtt ggatgaatgt tggttataat
      781 ggtccagata ttcataacat gatataaaat gacgaaatag attatatgaa atctcatcca
      841 gaataattta gaaaaaaatc gaaaaatatg agttgtttga gttaaaattt taactatgga
      901 gatagagtcc tgtattattt gattttgtag taaactagtt aggaatttat tcataggata
      961 ttataaagga gttaatgatg aagaaaaaat attggatatt actaatatta ctattaacag
     1021 ttttatcagg aggcggatat atggttttta aacaacatac agaaaaagaa
//
LOCUS       DATROD010000193         1050 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.197, whole genome shotgun sequence.
ACCESSION   DATROD010000193 DATROD010000000
VERSION     DATROD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.197"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            48..395
                     /locus_tag="VBC74_002033"
     CDS             48..395
                     /locus_tag="VBC74_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEO6942925.1"
                     /translation="MFTTFDRVKELSQKRGISLSKLEETLGLGKNSIYALKRNQPSAE
                     RLQEIADYFNVSTDYLLGRTDNPNIAKDNQEYTSDDLRKMAEKAKTFDGKPLTDSDID
                     AIQNIIEIYLKGR"
     gene            399..785
                     /locus_tag="VBC74_002034"
     CDS             399..785
                     /locus_tag="VBC74_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEO6942926.1"
                     /translation="MTIEEILDFHNVELAYFDNDLWPRPGIYVDEIKVVFVNKALSDE
                     SKKKVIFHELGHIDHDSNQYGRRHEEFELEANRFMIRCLLEDEFDEVEDKHEFNYLSF
                     MKRHNLKTTTDEVMVIDEYYNLLDAV"
     gene            910..>1050
                     /locus_tag="VBC74_002035"
     CDS             910..>1050
                     /locus_tag="VBC74_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEO6942927.1"
                     /translation="MAISIKSINLQNFRGFEDLHLNLNKYVTCISGHNGTGKSTILAA
                     LSN"
BASE COUNT          404 a          149 c          180 g          317 t
ORIGIN      
        1 attctctaaa tattttttgt cataccctag aaaggttgat aaaatcaatg tttacgactt
       61 ttgatagagt taaagaatta tcccaaaagc gaggaatttc cctaagtaaa ttagaggaaa
      121 ctctaggatt gggcaaaaat tctatctatg ctcttaaaag aaaccaacca tctgctgaaa
      181 ggttacaaga aatagcagac tacttcaacg tatctacaga ttacttatta ggtagaacag
      241 ataaccccaa tatagctaaa gataaccaag aatacacttc tgacgatctt agaaaaatgg
      301 cagaaaaggc taaaacattt gatggcaagc cacttactga ttcagatatt gatgcaatac
      361 aaaatattat tgaaatttat ttgaaaggaa gatagtctat gactattgaa gagattttag
      421 acttccataa tgtagaatta gcttatttcg acaatgattt gtggccacgt ccagggatat
      481 atgttgacga aataaaagta gtctttgtaa ataaagcact atcagatgaa tcaaaaaaga
      541 aagttatctt ccatgaactc ggacatattg accacgactc taaccaatat gggcgcagac
      601 acgaggaatt tgaacttgaa gctaatagat ttatgatcag gtgcttactt gaggatgaat
      661 ttgacgaagt agaagataaa catgagttta attacttatc ttttatgaaa aggcacaact
      721 taaaaaccac gactgatgaa gtcatggtta tcgacgagta ttacaatctg ctggatgcag
      781 tataatcaat ggtattgcgc tgattgtgga acaagttttg aatcttaata attcaattac
      841 attattaaat tagaaatata aaaattctaa ataaagtaag ctaattaggt tataaaaagg
      901 aaaagttata tggcgatttc aataaaaagt ataaatcttc aaaatttcag gggatttgaa
      961 gacttacatc ttaatttaaa taaatatgta acttgtattt ctggtcataa cggaactgga
     1021 aaatctacta tacttgcagc tcttagtaat
//
LOCUS       DATROD010000194          999 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.198, whole genome shotgun sequence.
ACCESSION   DATROD010000194 DATROD010000000
VERSION     DATROD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.198"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..>999
                     /locus_tag="VBC74_002036"
     CDS             <1..>999
                     /locus_tag="VBC74_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942928.1"
                     /translation="DSSYSSNINISEDKIKENDQKILDLIVKSGANNQNLTDEEKVIA
                     FTKYIGEITNYDNEAYRARNVDTEYYRASDLFSVTERKLAMCVGYSVTAARAFNIMGI
                     PSYVVSGKSPQGISHAAVRAYYNRSWHIIDITASTYWKNGNYKTTYSDFIKEYCIDGY
                     DVYDPAKTNNRFKVKYMESNEAFENWIHNNGSKSMLFINESAALKDKKPKDDFVPVPV
                     TEKEKNELIDKYKKLLSQIPENTQNPGEKNIRDYLKNEYEEILKKDNLFEHEHAEFKE
                     SLNLNESFYLQLKKEEKKPSDNLKKEEKPRENSVKERETPAENNDFVSVTEKNNLI"
BASE COUNT          443 a          122 c          165 g          269 t
ORIGIN      
        1 aagattcttc atattcttca aacattaata ttagtgaaga taaaataaaa gaaaatgacc
       61 aaaaaattct agatttaata gttaaatctg gagctaataa tcaaaactta acagatgaag
      121 aaaaagtaat tgctttcact aaatatatag gtgaaataac taattatgac aatgaagctt
      181 acagagctag aaatgtcgac acagaatatt acagagcttc agatttattt tctgtaacag
      241 aaagaaaact tgctatgtgc gttggctata gtgtaacagc cgctagagca tttaatatta
      301 tggggatacc tagttatgtc gtttctggta aaagtcctca aggaatatct catgcagctg
      361 tgagagcgta ttataataga agttggcata tcatagatat cacagccagt acatattgga
      421 aaaatggtaa ttacaaaaca acgtattccg attttattaa agaatactgt atagatggtt
      481 atgacgttta tgatcccgca aaaacaaata atagatttaa agttaagtat atggaatcaa
      541 atgaagcgtt tgaaaattgg atacataata atggaagcaa atctatgtta tttattaatg
      601 aatctgcagc tctaaaagat aaaaaaccaa aagatgattt tgtaccagta ccagtaacag
      661 aaaaagaaaa aaacgagctg atagataagt ataaaaaatt attgtcacag attcctgaaa
      721 acacacaaaa tccaggagaa aaaaacataa gagattattt gaaaaatgaa tatgaagaaa
      781 tattaaagaa agataattta tttgagcatg aacatgcaga atttaaagaa agtctaaatt
      841 taaatgaatc attctaccta caattaaaga aagaagaaaa gaaaccgagt gataatctca
      901 aaaaagaaga aaaaccaaga gaaaattcag taaaagagag agagactcca gctgaaaaca
      961 atgattttgt ttcagtaaca gaaaaaaaca acttgattg
//
LOCUS       DATROD010000195          997 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.199, whole genome shotgun sequence.
ACCESSION   DATROD010000195 DATROD010000000
VERSION     DATROD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.199"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..228
                     /locus_tag="VBC74_002037"
     CDS             <1..228
                     /locus_tag="VBC74_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEO6942929.1"
                     /translation="QRFNSLLQLAEADPSNYYHKFTQIPPHKAMELAKDIWKTINLVN
                     LEKYIEPTRNRADFIIHKGKHHKIDEIYLKK"
     gene            282..530
                     /locus_tag="VBC74_002038"
     CDS             282..530
                     /locus_tag="VBC74_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEO6942930.1"
                     /translation="MEVKTLANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANP
                     SEELYRAASSSIDKAASKGLIHTNKASRDKARLATKLG"
     gene            complement(614..>997)
                     /locus_tag="VBC74_002039"
     CDS             complement(614..>997)
                     /locus_tag="VBC74_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEO6942931.1"
                     /translation="KLGAQSGSSGFDAFNAKPDILKKFVKGKEAVQYDTFTQALIDLK
                     NNRIDGLLIDEVYANYYLKQEGNIKAYYFVKTAYQGENFVVGARKVDRRLIEKINKAF
                     KQLHNKGRFQKISYKWFGEDVYSKE"
BASE COUNT          370 a          181 c          133 g          313 t
ORIGIN      
        1 caacgattta acagtcttct acaattagcc gaagcagacc ctagtaatta ctatcataaa
       61 ttcacacaaa taccgccaca taaagctatg gaactagcta aagatatttg gaaaactatt
      121 aatttagtca accttgaaaa atatatagaa ccaaccagaa atagggctga ttttattatt
      181 cataaaggga aacatcataa aattgatgaa atttatctca aaaagtgata aagggttgcc
      241 aaaaatcact taattagata tacttatata gttagattaa tatggaggtg aaaacattgg
      301 caaatattaa atcagctatc aaacgcgctg aacttaacgt taaacaaaac gaaaaaaact
      361 cagcacaaaa atcagctatg cgtactgcta ttaaagcatt tgaagctaac ccttcagaag
      421 aactttaccg cgctgcttct tcaagcatcg acaaagctgc ttctaaaggt ttaattcaca
      481 caaacaaagc aagccgcgat aaagcacgtc ttgcaactaa acttggttaa ttaacctaga
      541 aaacatcgga tatatcgatg ttttttattt aattttttcg tagaataaaa gactaggaaa
      601 aattcctagt cttttattct ttactataaa catcttcacc aaaccatttg taagagattt
      661 tttgaaatct ccccttatta tgaagctgtt tgaaagcttt gttaatcttt tcaattagtc
      721 tacgatcaac tttacgagct cctactacaa aattttctcc ttgataagca gttttaacaa
      781 aataataagc ttttatattt ccttcttgct ttaaataata gttagcataa acttcatcaa
      841 tcaaaagacc atcaatacgg ttatttttta aatcaatcaa agcctgagtg aaagtatcgt
      901 attgaactgc ttcttttcct tttacaaact tttttaaaat atcaggttta gcgttaaaag
      961 catcaaaacc agatgaaccc gactgggctc ctagttt
//
LOCUS       DATROD010000196          947 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.201, whole genome shotgun sequence.
ACCESSION   DATROD010000196 DATROD010000000
VERSION     DATROD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.201"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(210..398)
                     /locus_tag="VBC74_002040"
     CDS             complement(210..398)
                     /locus_tag="VBC74_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, toxin component, MazF
                     family protein"
                     /protein_id="HEO6942932.1"
                     /translation="MSDIEVYSVLVSRIEYSDGTGSKVRPAVVVKFNDEVIKTLRFTT
                     KYENKSDNIRSQYLEVID"
     gene            complement(395..688)
                     /locus_tag="VBC74_002041"
     CDS             complement(395..688)
                     /locus_tag="VBC74_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEO6942933.1"
                     /translation="MSTVAVRVDDQLKDDATELFQSLGLDMSTAVKMFLIQSVKTQSI
                     PFEIKNKSSVSDEEFQNLVETKLKGIRVKASDPESVNAFFGDEDFSEYEEYFK"
     gene            745..894
                     /locus_tag="VBC74_002042"
                     /pseudo
     CDS             745..894
                     /locus_tag="VBC74_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          331 a          191 c           93 g          332 t
ORIGIN      
        1 aactaaaaat actgtagata tagctaaaat catcaatcct aataatttct ttttcataac
       61 taaacttcct ttgctatgag aaaatctttt aacctctcaa catctctatc gcttaactta
      121 cctataattt taatattgaa ccctttattc tcaacatcat aatattgaat agtatctatc
      181 caagaacgcc tatttaaatt tgcttttgcc taatcaataa cttctaaata ctgacttctt
      241 atattgtcag acttattttc atatttagta gtaaatctta aagtttttat tacttcatca
      301 ttaaatttga ctactacagc aggacgaacc tttgaacctg taccatcaga atattcaata
      361 cgagaaacta atacagaata tacctcaata tcactcattt aaaatattcc tcatattctg
      421 aaaaatcttc atctccaaaa aacgcattta cactttctgg atcactagcc ttaactcgta
      481 ttccctttaa ttttgtttct accaaatttt gaaattcttc atcagaaaca gatgatttat
      541 tttttatttc aaaaggaata ctttgagttt taacagattg aatcaaaaac atttttactg
      601 ctgtgctcat atccaaccct aaggattgaa aaagttctgt agcatcatct tttaattgat
      661 catcaactct tactgctact gttgacataa tatcacctca aatcattttt gtataactat
      721 tgtatcacta aatgcaatac aacgcaaaca aaaaaggaaa tcgtctttcc caatgaagag
      781 gctctagaat actatctagt cacactattt aatgattaca acttcaagca agaacaacga
      841 atccacaaag gttttggcca atgtacagac acacttgaaa gcctatttga ttaacaatcc
      901 cagctttgcc agtgtgttta cacaacatta ttgacacact cctttta
//
LOCUS       DATROD010000197          845 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.202, whole genome shotgun sequence.
ACCESSION   DATROD010000197 DATROD010000000
VERSION     DATROD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.202"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(149..703)
                     /gene="raiA"
                     /locus_tag="VBC74_002043"
     CDS             complement(149..703)
                     /gene="raiA"
                     /locus_tag="VBC74_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEO6942934.1"
                     /translation="MIKYSIRGENIEVTEAIREYVETKLSKVEKYFNEEQELDTRVNL
                     KVYREKTAKVEVTILIDSITLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKYRE
                     KIPASQVFTTEFEAEPDEEAVSQRIVRTKNVNLKPMDVEEALLQMELLGHDFFIYTDA
                     EDNTTNVLYKREDGELGLIEAKGN"
     gene            complement(780..>845)
                     /locus_tag="VBC74_002044"
     CDS             complement(780..>845)
                     /locus_tag="VBC74_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEO6942935.1"
                     /translation="QIRKLLEEKGIKNIKSFSLAR"
BASE COUNT          239 a          145 c          108 g          353 t
ORIGIN      
        1 acttttttta agttgtttat aaaatgataa tacaatatta ggttcgctta agaactcatt
       61 tagtatacta taatttttta tttgttgtca ataggtttta aaaaaatctc agaaaaaact
      121 ctgagatttt ttaaattatg ttacaaagtt aatttccttt agcttcaatt aaacctagtt
      181 cgccatcttc acgcttgtaa aggacatttg tcgtattatc ttctgcatct gtatagataa
      241 agaaatcatg acctaaaagt tccatttgca acaatgcttc ctcaacatcc attggtttta
      301 gattaacatt cttagtacgt acaattcttt ggcttactgc ttcttcatct ggctcagcct
      361 caaattctgt tgtgaaaact tgacttgctg gaattttttc acgatatttt ttcgcaattt
      421 tagttttatt tttacgaatt tgacgctcaa ttttatcaac aactaagtca attgacccat
      481 acatatcttg tgaaacatct tctgctcgta aagtaataga atctattaag attgttactt
      541 caacttttgc ggtcttctct ctgtatactt tgaggttgac tctagtatct aattcttgtt
      601 cttcattaaa gtatttttca actttagaga gtttggtctc aacatactca cgaatagctt
      661 ctgttacttc gatgttttca ccacgaatac tgtatttaat catatgagta cctctttctt
      721 gcgttgttaa cgctttctat attcttatta taaccgcttt catgaaaatt tgcaagattt
      781 tagcgtgcta atgaaaaact ttttatattt tttatacctt tttcttctaa tagtttcctg
      841 atttg
//
LOCUS       DATROD010000198          845 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.203, whole genome shotgun sequence.
ACCESSION   DATROD010000198 DATROD010000000
VERSION     DATROD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.203"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            280..>845
                     /locus_tag="VBC74_002045"
     CDS             280..>845
                     /locus_tag="VBC74_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEO6942936.1"
                     /translation="MKEKVTVIIPIYNSEAYLKECVQSVLQQTHPLIEVILIDDGSTD
                     NSGEICDNLSQEDNRILVFHKKNGGVSSARNLGLDKSTGEFITFVDSDDFVAPNMIEI
                     MLKNLITENADIAEVDFDISNERDYRKKKRRNFYKVFKNNNSLKEFLSGNRVENIVCT
                     KLYKKSIIGNLRFDENLKIGEDLLFNCKL"
BASE COUNT          311 a           96 c          151 g          287 t
ORIGIN      
        1 atttggacag ggtataagga ttccatcaag tgaaggaata ttcctaggat cgcattctac
       61 ttatattagt gtcttttaca ggacttcttt attaggaatt gttctttatt tttctgcctt
      121 tatactttta tataaagaag cgatttcaaa aaattataaa atctacagat tattttttta
      181 tacgttatta tgttacacgc tctttgagga aatagatcct aatcattgga gtattgtatt
      241 attattctca acttttggta tagtgggaag ggctaaaaaa tgaaagaaaa agtaacagtc
      301 attataccta tatacaactc agaagcatac cttaaagaat gtgtgcaatc cgtactacaa
      361 cagactcatc cattgataga agttatacta attgatgatg gatccactga taatagtgga
      421 gaaatttgtg ataatttatc tcaggaagat aatcgcatac ttgtatttca taaaaaaaat
      481 ggaggggtct cttcggcaag gaacctaggt ctagataaat ccacaggaga attcataaca
      541 tttgtggata gtgatgattt tgtagcaccg aatatgattg aaataatgtt aaaaaattta
      601 atcactgaga atgctgatat agcagaagta gattttgata tttcgaatga gagagattat
      661 agaaagaaga aaagacgaaa cttttataaa gtttttaaga ataataactc tttgaaagaa
      721 tttttatcag gtaatagagt ggaaaatatt gtttgtacaa aattatataa aaaaagtata
      781 attggtaact tgaggtttga tgagaactta aaaattggtg aggatttact ttttaattgc
      841 aaact
//
LOCUS       DATROD010000199          829 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.205, whole genome shotgun sequence.
ACCESSION   DATROD010000199 DATROD010000000
VERSION     DATROD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.205"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..>829
                     /locus_tag="VBC74_002046"
     CDS             <1..>829
                     /locus_tag="VBC74_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEO6942937.1"
                     /translation="SIISLSIKDATDRASKETFANITNSFSMEINRQVNPGTPRGGGN
                     VKGEDIKKISQTNSIDSYVKRINSVADLVDHDIIETQDTLANQSPERAKNFKRTVMLT
                     GVNDSAKETKFVSEAYKLVEGKHLENKDKNKILMHKDLAKKNNLKVGDKIKIKSNLFD
                     ADNEKVANETVEVEIKGLFDGHNSGGVSAAQELYENTLITDVHSAAKVYGNTEDTAVY
                     QDATFFVKGDKNLDSVIKDLGKLDINWREYNLIKSSSNYPALQQSISGIYSISNKLFV
                     "
BASE COUNT          354 a          105 c          146 g          224 t
ORIGIN      
        1 aagcatcatt agtttatcta ttaaagatgc cacagataga gcttctaagg aaacatttgc
       61 taatataaca aacagttttt ctatggaaat aaatagacaa gtaaatccag gaacacctag
      121 gggtggagga aatgtaaaag gtgaagatat taaaaagata tctcaaacaa acagtataga
      181 ctcctatgta aaaagaataa acagcgtcgc agacctagtt gatcatgata taattgaaac
      241 tcaagataca cttgcaaatc aatcacctga aagagcaaag aattttaaga gaacagttat
      301 gttaacaggg gttaatgact cagcaaaaga aacaaaattt gtatcagaag catataagtt
      361 agtagaagga aagcatttag aaaataaaga taaaaataaa attttaatgc ataaagattt
      421 ggcaaaaaaa aacaatctca aagttggaga taaaataaaa ataaaatcta atttgtttga
      481 tgctgacaat gaaaaagttg caaatgaaac tgtagaagta gaaattaaag gtttatttga
      541 tggtcataat agcggtgggg taagtgcagc acaagaacta tacgaaaata cactaatcac
      601 tgacgttcat tcagctgcta aagtttatgg taatacagaa gatacagccg tataccaaga
      661 tgcaacattc tttgtaaaag gtgataagaa tcttgatagt gtaataaaag atcttgggaa
      721 actagatata aattggagag aatataattt gattaaaagt tcatcaaatt atcctgcgct
      781 gcaacaatct atatccggta tctattctat ttcaaacaaa ttatttgtt
//
LOCUS       DATROD010000200          813 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.206, whole genome shotgun sequence.
ACCESSION   DATROD010000200 DATROD010000000
VERSION     DATROD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.206"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            301..813
                     /locus_tag="VBC74_002047"
                     /pseudo
     CDS             301..813
                     /locus_tag="VBC74_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          294 a          146 c          128 g          245 t
ORIGIN      
        1 atagagactt tttgtccaat gttgagcggt taaataactc tttatgagga atcattgcaa
       61 gaactgattt atcattcttt agtgatctta ttaattcttt tgaagagttg gtattatgct
      121 ttatcatatt acctaaaaag aataattttg ctgtgacata acttgctcga gttttaaagt
      181 caaatgcctc tgttttaaat tcttcaagtc tttcagaaag atggatgtat actataaaag
      241 tagacaaaac tttgtgtgtt acaatctgtt ttaaaagaca caaaaacgaa aggacaacct
      301 atgtcaacat ttaaacgcta tgatgaagaa ttcaaacaat ctcttgttaa cctttatcaa
      361 actggaaaaa ctcagtctga actctgtaaa gactatgggg tatccacttc tacgcttgca
      421 aaatggatca aacagtattc ccaagtcaaa ctcgaagata attctgtact tactgccaag
      481 caaatacaag agttacaaaa acgtaatgcg cagcttgaag aggagaacct tatcctaaaa
      541 aaagcaagtg ccatattcat gcaaaactta aagtaagact cctcgctgtc tatcggttac
      601 gctttgaaca cgccacaaca accttgtgtc gtgttttacg tgtcaatcgc tccacttact
      661 ataaatttct aaaacataag ccctcaaaaa gagaattgga taatcaaatt tatagaaaac
      721 aaatacttga gatttatacc aaagcgaaca aaagacttgg tgtgaagtct atcaaggtca
      781 ttcttcaaag agactatgac acaaaaatct ctg
//
LOCUS       DATROD010000201          789 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.208, whole genome shotgun sequence.
ACCESSION   DATROD010000201 DATROD010000000
VERSION     DATROD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.208"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            107..>789
                     /locus_tag="VBC74_002048"
     CDS             107..>789
                     /locus_tag="VBC74_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEO6942938.1"
                     /translation="MTKFIVDSSYWNLFPTSKIGVILIKDYHMDRNLETELKQLLSDS
                     HSLAKKYLQEKEFSQNKVIQTYRKAYQTFKTKKGARSSIEALLKRINSGNEITSINPL
                     VDIYNAASLRFGLPIGAEDSDTFRGDLKLTITNGGDEFYLIGEDFNIPTLSGELAYVD
                     DVGAVCRCFNWRDGKRTMITDNTQNAFLVIELIDNRREIIFKEALDFIATNTNRFLKA
                     KTQTIILDK"
BASE COUNT          282 a          116 c          137 g          254 t
ORIGIN      
        1 ttatagcaaa aaaattcgta gaaagaacgt ttttcgttga attttgaaaa cgcttttgag
       61 aaaatgtaat tgttctgata tcttttaaaa aaattaagag gccttcatga ctaaatttat
      121 tgttgattca tcgtactgga atttgtttcc aacttctaaa attggagtaa tcttaatcaa
      181 agattatcac atggacagga accttgaaac agaacttaaa cagttactgt ctgatagtca
      241 ttctttagca aaaaaatatt tgcaagagaa agaattcagt caaaacaaag ttatccaaac
      301 ttatcgcaaa gcatatcaaa cctttaaaac taaaaaaggt gcacgttcaa gtatcgaagc
      361 attattgaag cgaattaatt caggaaacga aattactagt ataaatcctc tagttgatat
      421 ctataatgcc gctagcctta ggtttggatt acctattggt gctgaggata gtgatacatt
      481 tagaggtgat ttaaaactaa ctattaccaa tggtggtgat gaattttact tgattggcga
      541 agattttaac ataccaactc tttcaggtga gcttgcctat gttgatgatg ttggagcagt
      601 ttgccgctgt tttaattggc gtgatggaaa acgtactatg attaccgata atactcagaa
      661 cgctttttta gttatcgaat taattgataa tagaagagaa attattttta aagaagcgtt
      721 ggactttatc gccaccaata caaatcgctt tctaaaagct aaaacacaga ccatcatatt
      781 agataaaga
//
LOCUS       DATROD010000202          778 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.209, whole genome shotgun sequence.
ACCESSION   DATROD010000202 DATROD010000000
VERSION     DATROD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.209"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..>778
                     /locus_tag="VBC74_002049"
     CDS             <1..>778
                     /locus_tag="VBC74_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEO6942939.1"
                     /translation="VIREYKSEKTISLFYNHYLILSNFSKTQHDFLFHYGSLGELFLV
                     HHIQQYYIIIGPWRSNVIDPLLLKKKLTETQINASEQDYFIDRLSQLPFFSLSQIREL
                     LIVTNYCLTGVVKDKLSEPLHYYTKGWSNSFDLDKIKQFSKQNMSSYKYQYHFENNIL
                     KAVKSGSEFLLKETVEQFSNSIVPIISGDELRSEKNYSIMIYDRLSQATIQAGLDIET
                     AYRARDRFIKENESTISLNEVLKLRDTAILFYTQQVHSLKK"
BASE COUNT          276 a          137 c          109 g          256 t
ORIGIN      
        1 gttatcagag agtataaatc cgagaaaact atttcacttt tttacaatca ttaccttatt
       61 ttaagtaact ttagtaaaac tcaacatgat tttttatttc attatggctc tttaggagaa
      121 ctttttcttg tccaccatat tcaacaatat tatattatca tcggtccctg gcgtagcaat
      181 gttattgacc ctttacttct taagaaaaag ctgactgaaa cacaaatcaa tgctagtgaa
      241 caagactact ttattgatag actatcccaa ttacctttct tctcacttag tcaaatccga
      301 gaattactaa tagtaaccaa ttattgtcta actggggttg tgaaagataa attatcagag
      361 cccttacatt actacacgaa agggtggagc aactcctttg atctagataa aattaagcaa
      421 ttttctaaac agaatatgag ttcttacaaa tatcagtatc attttgaaaa taatattcta
      481 aaagctgtta aatcaggtag tgaatttctt ttgaaagaaa cagtggaaca atttagcaat
      541 tctatcgtac ctataatcag tggagatgaa ttgcgatctg aaaaaaacta ctccatcatg
      601 atttatgatc gtctctcaca ggctaccatc caagcaggac ttgatatcga aacagcttat
      661 cgagcacgag atcgttttat aaaagaaaac gagtcaacta taagtctaaa tgaagtttta
      721 aaattacgtg atactgctat cttattctat actcaacaag ttcattcttt aaaaaaac
//
LOCUS       DATROD010000203          744 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.211, whole genome shotgun sequence.
ACCESSION   DATROD010000203 DATROD010000000
VERSION     DATROD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.211"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
BASE COUNT          269 a          104 c          135 g          236 t
ORIGIN      
        1 aagtcagcta accgatcttt ctattctaaa acttatattg ttgctttaga aattttaaag
       61 agcgtggatg gcttcataat aaacagaaat cttaccaatt tgcgaagcca aaaaatatga
      121 tatttgaaga gtccaaagat ctaatagata gtccagttag agaagcgttg attataagtg
      181 ataaggattt tcaaaaatta aaacaagagc tattatttta accgacttat tttaaagact
      241 tatcatatct aggcttgctt gatgattcgg aaaaatacgg agactatact atttcaagga
      301 aaagatacaa aagtttcgaa tcaagtcttc aactatacat ccttcaaagt catcggctag
      361 agatttggaa ttatgaacca atccctttga ttactagaaa aataaatagc ttggagaggc
      421 taacgtgaca ctggttgatc caatctcgct ttatttaaca ctaagaatga tgaagaccct
      481 cgtattgaag aagaagttga gcagctagaa gataagattt tgagaatata atcgtttttt
      541 cgtgctacac taacttgtca gggttgcaac agacaaaaaa ataatttagg agggattcgt
      601 tatgcgtaca aagtatatag gatatgaaat tgttttaagg ggtggttatt aatatggata
      661 agaaaaaggt tgtttcaact atcgtatcta cagggtttct tatagtttca atatcaaatt
      721 tttttagaga gctatcacct atac
//
LOCUS       DATROD010000204          734 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.213, whole genome shotgun sequence.
ACCESSION   DATROD010000204 DATROD010000000
VERSION     DATROD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.213"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..545)
                     /locus_tag="VBC74_002050"
     CDS             complement(<1..545)
                     /locus_tag="VBC74_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEO6942940.1"
                     /translation="MNSLDNLLSHNLEDNRHLLPYEHMHTEVRNGYPDILFHWHPELE
                     ISYVHEGTARYHIDYDFFNSQSGDIILIRPNGMHSIHPIENKEHITDSIKFHLDLIGY
                     SIVDQVSLRYLQPLQTSSFKFIQCIKPSMTGYNDIKNCLFDIFNISKEENRHFELLLK
                     AKLNELLYLLYYHQYVIKKHT"
BASE COUNT          259 a           95 c          124 g          256 t
ORIGIN      
        1 tcagtatgtt ttttaatgac atattgatga taatagagta aatacaagag ttcgtttaat
       61 ttagctttta ggagtagctc aaaatgtcga ttttcttctt ttgaaatatt aaaaatatca
      121 aacaaacaat ttttgatatc attatatcct gtcatactag gtttgataca ttgaataaac
      181 ttaaaagagc tagtctgcaa cggttgtaaa taacgaaggc taacttggtc tacaatagaa
      241 tatcctatta aatctaagtg aaatttaatg ctatctgtga tatgctcttt attttcaatc
      301 ggatgaatag aatgcatacc atttggacgg atcaagataa tatcaccaga ttggctatta
      361 aaaaaatcat aatcgatgtg ataacgtgcg gtcccctcgt ggacatatga aatttcaagt
      421 tccggatgcc agtgaaataa aatgtcaggg taaccatttc taacctcagt atgcatgtgt
      481 tcatagggta ataaatgccg gttatcttct aaattatgtg atagtaaatt atcaagtgag
      541 ttcatcgttt ttctttctaa aatcgtagga tagtattatt ataacagaaa actatcaaaa
      601 aatgggtttc gtaggatagt attatttttt gaaaggatag tgtaagtttt gtataggaac
      661 atgacatata atataagtca tagagaaaac gctttaaaat ttttgcttag agtgtttcta
      721 ataacttcaa aatc
//
LOCUS       DATROD010000205          727 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.214, whole genome shotgun sequence.
ACCESSION   DATROD010000205 DATROD010000000
VERSION     DATROD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.214"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            132..>727
                     /locus_tag="VBC74_002051"
     CDS             132..>727
                     /locus_tag="VBC74_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEO6942941.1"
                     /translation="MNHQILQNYINSHNLPIIEKDYHKYLTFESLEEDYTYILKDGIV
                     KQSVLSKYGTEFNLRYVTGLEITSILNTDYSQHMGEPYNVRIESETAHFYKVRRSTFL
                     KDINNDIELQGYVKDFYHNRLEKSMKKMQCMLTNGRIGAISTQLYDLSKMFGEERDNG
                     DIYINFVITNEELGKFCGISTGSSVSRILKQLKDDHIIR"
BASE COUNT          270 a          124 c          114 g          219 t
ORIGIN      
        1 tcattttcat aaacatttta caaaaatatt agaagatacg atatttcaat ggtataatat
       61 aagtgctagt atcagactgc attaacaaat gttaaggtgg tctatcaaac ctaaaacatt
      121 tggaggaagt catgaatcat caaattttac aaaattacat taatagtcat aatcttccaa
      181 ttatcgaaaa agactaccat aaatacctta catttgaaag tttagaagaa gattatactt
      241 atatcctaaa agatggtatt gttaaacaaa gtgttctctc taaatatggt actgagttca
      301 atctgcgtta tgtgacaggc ttagaaatca cttctatcct aaatacagac tactcccaac
      361 acatgggaga accttacaac gttcgaatcg aatccgaaac tgcccacttt tacaaagtac
      421 gtcgctctac cttcctaaaa gatatcaata atgatattga actgcaagga tatgtgaaag
      481 atttctatca taaccgcctt gaaaaatcta tgaaaaaaat gcaatgtatg ctcactaatg
      541 gtcgaattgg tgctatctcc acacaattat atgacctcag taagatgttt ggtgaagaac
      601 gagataacgg tgatatctat attaattttg ttatcacgaa cgaagaactt ggtaaatttt
      661 gcggaatatc cacaggaagt agcgtgagcc gcattttgaa acaactaaaa gacgatcata
      721 ttattcg
//
LOCUS       DATROD010000206          685 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.216, whole genome shotgun sequence.
ACCESSION   DATROD010000206 DATROD010000000
VERSION     DATROD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.216"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..684)
                     /locus_tag="VBC74_002052"
     CDS             complement(<1..684)
                     /locus_tag="VBC74_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-like surface protein"
                     /protein_id="HEO6942942.1"
                     /translation="MFRRSKNNSYDTLQTKQRFSIKKFKFGAASVLIGISFLGGFTQG
                     QFNISTDTVFAAEVISGSAVTLNTNMTKNVQNGRAYIDLYDVKNGKIDPLQLITLNSP
                     DLKAQYVIRQGGNYFTQPSELTTVGAASINYTVLKTDGSPHTKPDGQVDIINVSLTIY
                     NSSALRDKIDEVKKKAEDPKWDEGSRDKVLISLDDIKTDIDNNPKTQSDIANKITEVT
                     NLEKILVPRI"
BASE COUNT          211 a          130 c           91 g          253 t
ORIGIN      
        1 gattcgaggt actagtattt tttctaaatt agtaacttca gttattttat tggcaatgtc
       61 tgattgcgtc ttaggattat tatcaatatc tgttttgata tcatctaaac ttatcaaaac
      121 tttatcgcga cttccctcgt cccatttagg gtcttccgct ttctttttaa cttcatctat
      181 tttatctctc aaagctgaag aattgtaaat agtcaatgaa acgtttataa tatccacttg
      241 tccatcaggc ttcgtatgag gacttccatc tgtcttcaat actgtataat taatactagc
      301 tgcaccaaca gtagtcaatt cagaaggttg tgtgaaataa ttgccgcctt gcctaatgac
      361 atactgagct tttaaatcag gtgaatttaa cgtaattaat tgtaatggat ctattttccc
      421 atttttcaca tcatataaat ctatatatgc tctaccattc tgaacatttt tagtcatatt
      481 tgtgtttaac gtaacagcac ttcctgaaat tacttcagct gcaaacacag tatctgtaga
      541 aatattaaat tgcccttgag taaaacctcc taaaaaacta ataccaatta gtacagaagc
      601 tgcaccaaac ttaaacttct taattgaaaa ccgttgtttc gtctgtaaag tatcataact
      661 gttattttta gaccttctaa acata
//
LOCUS       DATROD010000207          680 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.217, whole genome shotgun sequence.
ACCESSION   DATROD010000207 DATROD010000000
VERSION     DATROD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.217"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(78..151)
                     /locus_tag="VBC74_002053"
     tRNA            complement(78..151)
                     /locus_tag="VBC74_002053"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(115..117),aa:Arg,seq:acg)
     gene            complement(163..247)
                     /locus_tag="VBC74_002054"
     tRNA            complement(163..247)
                     /locus_tag="VBC74_002054"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(211..213),aa:Leu,seq:taa)
     gene            complement(256..327)
                     /locus_tag="VBC74_002055"
     tRNA            complement(256..327)
                     /locus_tag="VBC74_002055"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(293..295),aa:Gly,seq:gcc)
     gene            complement(361..433)
                     /locus_tag="VBC74_002056"
     tRNA            complement(361..433)
                     /locus_tag="VBC74_002056"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(398..400),aa:Thr,seq:tgt)
     gene            complement(444..525)
                     /locus_tag="VBC74_002057"
     tRNA            complement(444..525)
                     /locus_tag="VBC74_002057"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(489..491),aa:Leu,seq:tag)
     gene            complement(535..607)
                     /locus_tag="VBC74_002058"
     tRNA            complement(535..607)
                     /locus_tag="VBC74_002058"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(572..574),aa:Lys,seq:ttt)
     gene            complement(610..680)
                     /locus_tag="VBC74_002059"
     tRNA            complement(610..680)
                     /locus_tag="VBC74_002059"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(647..649),aa:Asp,seq:gtc)
BASE COUNT          183 a          199 c          150 g          148 t
ORIGIN      
        1 gaagacagga ttcgaacctg cgacaccttg gtcccaaacc aagtactcta ccaagctgag
       61 ctacttcccg aaaaatatgc accctagagg agtcgaacct ctaaccgcct gattcgtagt
      121 caggtactct atccagttga gctaagggtg ctaaatatta tatgccgagg accggaatcg
      181 aaccggtacg atgtttacca tcgcaggatt ttaagtcctg tgcgtctgcc agttccgcca
      241 ccccggcctc taacaagcga acgacggggt tcgaacccgc gaccctcacc ttggcaaggt
      301 gatgttctac cactgaacta cgttcgcact aaagacacta tttatcctat aaaattgtaa
      361 tgccggctac atgacttgaa cacgcgaccc tctgattaca aatcagatgc tctaccaact
      421 gagctaagcc ggcaatctac taatgcgggt gaagggactt gaacccccac gccgttaagc
      481 gccagatcct aaatctggtg cgtctgccaa ttccgccaca cccgcatttc taaatgaccc
      541 gtactgggct cgaaccagtg acccattgat taaaagtcaa ttgctctacc aactgagcta
      601 acgagtctac ggtcccgacg ggaatcgaac ccgcgatctt cgccgtgaca gggcgacgtg
      661 ataaccgcta cactacggga
//
LOCUS       DATROD010000208          555 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.223, whole genome shotgun sequence.
ACCESSION   DATROD010000208 DATROD010000000
VERSION     DATROD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.223"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..>555
                     /locus_tag="VBC74_002060"
     CDS             <1..>555
                     /locus_tag="VBC74_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSag9 family transposase"
                     /protein_id="HEO6942943.1"
                     /translation="KTFYLAKQAQRNYEKLLVESREGIPLNKETFWKIDRVLSNGVKK
                     GQRIYHILKTNDLEVSSSTVYRHIKKGYLSITPIDLPRAVKFKKRRKSTLPPIPKAIK
                     EGRRYEDFIEHMNQSELNSWLEMDTVIGRIGGKVLLTFNVAFCNFIFAKLMDSKTAIE
                     TAKHIQVIKRTLYDNKRDFFELFPV"
BASE COUNT          187 a          124 c          107 g          137 t
ORIGIN      
        1 aagaccttct atcttgctaa gcaagctcaa agaaactatg aaaaactttt agttgaatct
       61 agagaaggaa tccctttgaa caaagagacc ttctggaaaa tagatagagt gctttctaat
      121 ggggtcaaga agggccaacg tatctatcat atcctcaaaa ccaacgatct agaagtgagt
      181 tcttcaaccg tttatcgaca catcaagaaa ggctacctat ccatcacacc aatcgaccta
      241 cccagagctg tgaagttcaa aaaaaggcga aagagcacac tccctcctat tccaaaagcg
      301 attaaagaag ggcgacggta cgaggatttt atagaacaca tgaaccaatc agagctgaac
      361 tcctggcttg aaatggacac ggttattgga cggattggtg gaaaggtcct tctcaccttc
      421 aatgtcgcct tctgtaactt catctttgcc aaactgatgg attctaagac agctatcgaa
      481 actgctaaac acatacaggt cattaagagg acactctatg ataacaagag agacttcttc
      541 gaactctttc ctgtt
//
LOCUS       DATROD010000209          504 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.226, whole genome shotgun sequence.
ACCESSION   DATROD010000209 DATROD010000000
VERSION     DATROD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.226"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..>504
                     /locus_tag="VBC74_002061"
     CDS             <1..>504
                     /locus_tag="VBC74_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001901474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEO6942944.1"
                     /translation="ETAIVQLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNIIQ
                     DGDTESLIRKYFHSGVVINGQRHKTLVGTPQGGNLSPLLSNIMLNELDKGLEKRGLRF
                     VRYADDCVITVGSEAAAKRVMHSVSSYIEKRLGLKVNMTKTKIVRPNKLKYLGFGFWK
                     SPKGWKCR"
BASE COUNT          143 a           99 c          124 g          138 t
ORIGIN      
        1 gaaacagcca ttgttcagct acttgagtat ttaaacgatg gctacgagtg gattgtggac
       61 attgacttgg aaaagttctt cgatactgtt ccgcaagaca gattgatgtc cctggttcat
      121 aatatcattc aagatggcga tacggagtca ctgattcgta agtacttcca ttcgggagtt
      181 gttattaacg gacagcgaca taagacttta gtcgggacac ctcaaggcgg gaatctatca
      241 cccctcctat ctaatattat gcttaatgag ttagacaaag ggttggaaaa gcgaggtctt
      301 cgctttgtcc gttacgccga tgactgtgtc atcactgtcg gaagcgaagc agctgctaag
      361 cgggtcatgc attcggtcag tagctatatt gagaagcgat tagggttgaa agtcaacatg
      421 actaagacca agattgtcag accgaacaaa ctcaaatacc tcggatttgg tttctggaaa
      481 tctccaaaag gttggaagtg tcgt
//
LOCUS       DATROD010000210          489 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.229, whole genome shotgun sequence.
ACCESSION   DATROD010000210 DATROD010000000
VERSION     DATROD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.229"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..484)
                     /locus_tag="VBC74_002062"
     CDS             complement(<1..484)
                     /locus_tag="VBC74_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017649420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEO6942945.1"
                     /translation="MSRSQKVILTNMCLIEDNKGRIVMQIRDPKRYSWSGAALPGGHI
                     EEGESLYDALVREVREETGLTISNPKLVGMKHWYTDEGIRYLVFLYKTYDFSGELQSS
                     DEGDVIWIERSQLDKIELAYDMLNLLRVFDEDDISEMFYSKRLEDGFIRKILLNKKEL
                     G"
BASE COUNT          151 a          105 c           65 g          168 t
ORIGIN      
        1 ttcctagctc ttttttgttt aataaaattt ttcttataaa accatcttca agtcgcttac
       61 tataaaacat ctctgagatg tcatcttcgt caaatactct caaaaggtta agcatatcat
      121 atgctagttc aatcttatca agttgtgaac gttcaatcca tataacatca ccttcatctg
      181 acgattgtaa ttcacctgaa aaatcatatg ttttataaag gaataccaaa taacggatgc
      241 cttcatctgt gtaccaatgc ttcataccaa ctaatttggg attacttatt gttaaacctg
      301 tctcctcgcg aacttctcga acgagagcat catacaagct ttctccttct tcaatatggc
      361 cgcctggtag agcagctcct gaccaagaat aacgtttagg atcacggatt tgcatcacaa
      421 tacgtccttt attatcttca ataagacaca tattagttaa aataactttt tgactgcgtg
      481 acattattt
//
LOCUS       DATROD010000211          460 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.231, whole genome shotgun sequence.
ACCESSION   DATROD010000211 DATROD010000000
VERSION     DATROD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.231"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(27..>460)
                     /locus_tag="VBC74_002063"
     CDS             complement(27..>460)
                     /locus_tag="VBC74_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEO6942946.1"
                     /translation="NVKSNQECPKLWEKLHKQYNPSKLKEKGIQQAIGICKTQPDFRF
                     DYSATYQVETSVQAPKGLEIIRIPSATYAVISVKGPMPSSLQETWRKIIQGFFQENNL
                     KPANSPNLEIYSSQHPQDTDYQMEIWLAIADITNNSKTNLI"
BASE COUNT          122 a           66 c           89 g          183 t
ORIGIN      
        1 aaggggcttt tttatattaa gcaatactag attaagtttg ttttcgagtt attagtaata
       61 tctgcgatag caagccaaat ttccatttga taatcagtat cttgtggatg ttgagatgaa
      121 taaatttcta agtttggcga gttagctggt tttagattat tctcttgaaa aaatccttgt
      181 attatttttc gccatgtttc ttgaagactc gatggcattg gacctttaac agaaatgact
      241 gcataggtag cgcttgggat cctaataatt tctaaccctt ttggggcttg gacacttgtc
      301 tctacttgat aagtggcact gtaatcgaaa cgaaaatcag gttgtgtttt acaaatgcca
      361 atggcttgct ggataccttt ttcttttagt tttgatggat tatattgttt atgtaatttt
      421 tcccaaagtt taggacattc ttgatttgac tttacatttt
//
LOCUS       DATROD010000212          450 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.232, whole genome shotgun sequence.
ACCESSION   DATROD010000212 DATROD010000000
VERSION     DATROD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.232"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..>450
                     /locus_tag="VBC74_002064"
     CDS             <1..>450
                     /locus_tag="VBC74_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEO6942947.1"
                     /translation="KNMISQKMVTINIPHITTDMEVKNSLKMAFKEKVDVPAKYVSAA
                     KAKGPFLAGVNETIPYEAFGGDGMLTRLILKASEGAKWSDNGVDKNSPLLPLKGLTKG
                     KYFYQVSLNGNTTGKEGQALLDQIKANDKHSYQATIRVYGAKDGKVD"
BASE COUNT          171 a           67 c           88 g          124 t
ORIGIN      
        1 aaaaaacatg attagccaaa aaatggtaac cattaacatt cctcacatta caactgatat
       61 ggaagtaaaa aattctctaa agatggcctt taaagaaaaa gtagacgtcc cagcaaaata
      121 tgttagtgct gcaaaagcta aaggtccatt tttagcaggt gttaacgaaa cgattccata
      181 tgaagcgttt ggtggagatg gcatgttaac gcgtttaatc cttaaagctt cagagggagc
      241 aaaatggtca gacaatggtg ttgataaaaa tagtccatta ttaccattga aaggcttaac
      301 taaaggtaaa tatttctatc aagtttcttt aaatggtaat acgacaggta aagaggggca
      361 agctctctta gatcaaatta aagctaatga taaacatagc taccaagcca caattagagt
      421 ttatggtgct aaagatggta aagttgattt
//
LOCUS       DATROD010000213          431 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.233, whole genome shotgun sequence.
ACCESSION   DATROD010000213 DATROD010000000
VERSION     DATROD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.233"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..118
                     /locus_tag="VBC74_002065"
     CDS             <1..118
                     /locus_tag="VBC74_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001920755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEO6942948.1"
                     /translation="KGNKLPATGENATPFFNVVALTIMSSVGLLSVSKKKED"
BASE COUNT          141 a           67 c           64 g          159 t
ORIGIN      
        1 taaaggaaat aaactaccag caacaggtga gaatgcaact ccattcttta atgttgtagc
       61 tttgacaatt atgtcatcag ttggtttatt atctgtttct aagaaaaaag aggattaatc
      121 ttttgaccta aaatgtcact aaacttttca ccatttattg gtgtgaacac attaataaag
      181 ttatgtatct ctcttcaaca aacttaatta aagtgtttca atttttcgag tttaattctt
      241 gaaaaatcct atcgagatta ttaatttcga taggattttg attttgtgta agcgtccaat
      301 aacgaggtat cttccatttt cttccctatt ccgtaagcat ccaatatacc ttgttattgg
      361 acgcttacta taaaactgaa gaaatgatcc tatttagttg aaatttgtaa atgaagtgta
      421 tcattagagc c
//
LOCUS       DATROD010000214          425 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.234, whole genome shotgun sequence.
ACCESSION   DATROD010000214 DATROD010000000
VERSION     DATROD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.234"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..380
                     /locus_tag="VBC74_002066"
     CDS             <1..380
                     /locus_tag="VBC74_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001901474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEO6942949.1"
                     /translation="LTMRKWSIDLITRIERLNWVIRGWINYFSLGNMKSIMTQIDERL
                     RTRIRVIIWKQWKKKAKRLWGLLKLGVARWIADKVSGWGDHYQLVAQKSVLKRAISKP
                     ALAKRGLVSCLDYYLERHALKVS"
BASE COUNT          129 a           79 c          116 g          101 t
ORIGIN      
        1 aactgacgat gaggaaatgg agcattgacc tgataactcg cattgaacga ttgaactggg
       61 tcattcgagg atggataaac tatttctcgc ttggcaatat gaagagtatc atgacacaaa
      121 tagatgagcg tctgcgaacc cgtattcgag tgattatctg gaagcaatgg aagaagaaag
      181 caaagcgcct atggggactc ttaaaactag gagttgctag atggatagcc gataaagttt
      241 ctggatgggg tgaccactat cagttggtag ctcagaagtc ggtactcaaa cgtgctatat
      301 caaaaccagc cctcgcaaag cgaggactgg tcagttgctt agattactat cttgaacgac
      361 atgcgttaaa agttagttga accgccgtat gccgaacggc acgtacggtg gtgtgagagg
      421 ggcta
//
LOCUS       DATROD010000215          397 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.238, whole genome shotgun sequence.
ACCESSION   DATROD010000215 DATROD010000000
VERSION     DATROD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.238"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            90..>397
                     /locus_tag="VBC74_002067"
     CDS             90..>397
                     /locus_tag="VBC74_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001901474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942950.1"
                     /translation="MSELLDKISSRNNMLEAYKQVKSNKGSAGIDGVTIEQMDDYLHQ
                     NWRETKKLIKERSYKPQPVLRVEIPKPNGGVRNLGIPTAMDRMIQQAIVQVLSPLCEK
                     "
BASE COUNT          143 a           79 c           90 g           85 t
ORIGIN      
        1 tagaactaga tgcggttacg tattgactag aggaaggttc accaatgtaa gatatcatgc
       61 agacaacgaa aaaagaaagg aatacgcaca tgtcagaatt gctagataag atatcatctc
      121 ggaacaatat gctcgaagct tacaagcaag tgaaatcaaa caaaggttct gctggtatcg
      181 atggggtcac tatcgagcag atggatgact atcttcacca aaattggcga gaaaccaaga
      241 aactcatcaa agagaggagc tataaacctc aaccggttct cagggttgaa atcccaaaac
      301 caaacggagg agttcgtaac ctaggtatcc cgacggctat ggatagaatg attcagcagg
      361 ccatcgttca agttttgagt ccactctgcg aaaaaca
//
LOCUS       DATROD010000216          377 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.239, whole genome shotgun sequence.
ACCESSION   DATROD010000216 DATROD010000000
VERSION     DATROD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.239"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            complement(<1..>377)
                     /locus_tag="VBC74_002068"
     CDS             complement(<1..>377)
                     /locus_tag="VBC74_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HEO6942951.1"
                     /translation="NHKDYEKIMIKALIFSIDQSIEPNQEELARLTDYLFTVEQWGYY
                     EIILLGNCSRLINYNTLFLLTKEMVNSFAYSEQNKTNKILVTHLAINCLIISIDHSYF
                     EHSHYLIDKVRSLLQDEVNFYEKT"
BASE COUNT          127 a           54 c           59 g          137 t
ORIGIN      
        1 gttttttcat agaaattaac ttcatcctgt aacaatgatc taaccttatc aattaagtag
       61 tggctatgct caaagtaaga gtgatcaatg ctgatgatta gacaattaat tgctaagtgt
      121 gtaactagga ttttattggt tttattttgt tctgaataag caaacgaatt aaccatttct
      181 ttggtcaata aaaataaagt attgtaatta atgagacgtg agcaatttcc taagagaata
      241 atttcataat atccccactg ctccaccgta aaaagatagt ctgttaagcg tgctaattct
      301 tcttgattag gctctattga ttgatcaatt gaaaaaataa gagccttaat cataatcttt
      361 tcatagtctt tatggtt
//
LOCUS       DATROD010000217          371 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.denovo.240, whole genome shotgun sequence.
ACCESSION   DATROD010000217 DATROD010000000
VERSION     DATROD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.denovo.240"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            <1..>371
                     /locus_tag="VBC74_002069"
     CDS             <1..>371
                     /locus_tag="VBC74_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEO6942952.1"
                     /translation="DFKKYMSKDDYTNYITLFEDSGWKHISGSRWGGLHYFQKIKPET
                     SSEIFSDQESKSAMAMSYYKYTCEHAFFSLFYFFLLIRTTSITIFDPKSWYLTQGLWE
                     MQGKIFWLGFLFETPFALLRI"
BASE COUNT          120 a           52 c           71 g          128 t
ORIGIN      
        1 ggattttaaa aaatacatgt cgaaagatga ctatactaac tatatcactt tatttgagga
       61 cagtggatgg aagcatatta gcggtagtcg ttggggaggg ctgcattatt ttcaaaaaat
      121 aaagcctgaa accagttcag agattttttc tgatcaagaa tcaaaatcag cgatggctat
      181 gagttattat aaatatactt gtgagcatgc gtttttctct ttattttatt tctttctctt
      241 gataagaaca acttctatca ccatatttga tccaaaaagt tggtatctga cgcaaggtct
      301 atgggaaatg caagggaaaa tattttggtt agggttctta tttgaaacac catttgcctt
      361 attaagaata t
//
LOCUS       DATROD010000218         6785 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.guided.090, whole genome shotgun sequence.
ACCESSION   DATROD010000218 DATROD010000000
VERSION     DATROD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.guided.090"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            39..1958
                     /gene="tet(M)"
                     /locus_tag="VBC74_002070"
     CDS             39..1958
                     /gene="tet(M)"
                     /locus_tag="VBC74_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO6942953.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2077..2244
                     /locus_tag="VBC74_002071"
     CDS             2077..2244
                     /locus_tag="VBC74_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO6942954.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2304..2657)
                     /locus_tag="VBC74_002072"
     CDS             complement(2304..2657)
                     /locus_tag="VBC74_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6942955.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            3162..3485
                     /locus_tag="VBC74_002073"
     CDS             3162..3485
                     /locus_tag="VBC74_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO6942956.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYLW
                     EFRYF"
     gene            3464..3793
                     /locus_tag="VBC74_002074"
     CDS             3464..3793
                     /locus_tag="VBC74_002074"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942957.1"
                     /translation="MGISLFLELPAIVSYWVVTVMCFVAMGVSTMFVVQIYTLVQTQT
                     PPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVASIASIMISIYSKR
                     IFQKLEI"
     gene            4020..4439
                     /locus_tag="VBC74_002075"
     CDS             4020..4439
                     /locus_tag="VBC74_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6942958.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDRKISELYHVSRSAICSRRTRGLRKLKMLLSEGK"
     gene            4444..4683
                     /locus_tag="VBC74_002076"
     CDS             4444..4683
                     /locus_tag="VBC74_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6942959.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(4868..5434)
                     /locus_tag="VBC74_002077"
     CDS             complement(4868..5434)
                     /locus_tag="VBC74_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO6942960.1"
                     /translation="MIKKNKVFLGVLLVLVVILGGGVLFYQSQFQKTTNQALAIAYKD
                     AKVAKKDVIHQKIDKEFENFRGSYEIEFNTKSAEYSYHVDAKTGQILERDMDNNGFSK
                     STSQSSSSSSQKSHKISQEEAKKIAFKDANIEESEVSNLKIKEEIENGKSVYDIDFVD
                     LKNKNEVDYQIDAETGKIIERSRDHMND"
     gene            complement(5601..6620)
                     /locus_tag="VBC74_002078"
     CDS             complement(5601..6620)
                     /locus_tag="VBC74_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEO6942961.1"
                     /translation="MAKTALNIPFTVEGIENYYQNIKMILSLADKWSIPKDDLLLFLK
                     GLKAIDDIKVGSPIFKYFWTSLSLQAPLKALEFVLEQAKMPTELSGELSETQYLVAQF
                     SDELAPHDDFWIALSQVIYDSFPGNSLAEDTVLNRKLHQFRYLISSQQAQYVRRYFKD
                     VGMTDRDALVNYLSCLREPDSIAYYESARLHNKRRRNGEIFGFPDDEPVINSKLLISF
                     HTEFIIDDKGNFLNEIDAEVITRNGIINGASFNYAFKNNTRHKELDVDPVKLDPKFRN
                     DMTRGYRSPNLSRRKWFFFKEEDYDCSYFNKKGYYAFGRRSAKQSVDKQVKYLKKAVQ
                     KMRIN"
BASE COUNT         2229 a         1089 c         1233 g         2234 t
ORIGIN      
        1 tttatgccct tttgggcttt tgaatggagg aaaatcacat aaaaattatt aatattggag
       61 ttttagctca tgttgatgcg ggaaaaacta ccttaacaga aagcttatta tataacagtg
      121 gagcgattac agaattagga agcgtggaca aaggtacaac gaggacggat aatacgcttt
      181 tagaacgtca gagaggaatt acaattcaga caggaataac ctcttttcag tgggaaaata
      241 cgaaggtgaa catcatagac acgccaggac atatggattt cttagcagaa gtatatcgtt
      301 cattatcagt tttagatggg gcaattctac tgatttctgc aaaagatggc gtacaagcac
      361 aaactcgtat attatttcat gcacttagga aaatggggat tcccacaatc ttttttatca
      421 ataagattga ccaaaatgga attgatttat caacggttta tcaggatatt aaagagaaac
      481 tttctgccga aattgtaatc aaacagaagg tagaactgta tcctaatatg tgtgtgacga
      541 actttaccga atctgaacaa tgggatacgg taatagaggg aaacgatgac cttttagaga
      601 aatatacgtc tgggaaatta ttggaagcat tagaactcga acaagaggaa agcataagat
      661 ttcataattg ttccctgttc cctgtttatc acggaagtgc aaaaaacaat atagggattg
      721 ataaccttat agaagtgatt acgaataaat tttattcatc aacacatcga ggtcagtctg
      781 aactttgcgg aaaagttttc aaaattgagt attcggaaaa aagacagcgt cttgcatata
      841 tacgtcttta tagtggcgta ctgcatttgc gagattcggt tagaatatcg gaaaaggaaa
      901 aaataaaaat tacagaaatg tatacttcaa taaatggtga attatgtaaa atcgataagg
      961 cttattccgg ggaaattgtt attttgcaaa atgagttttt gaagttaaat agtgttcttg
     1021 gagatacaaa actattgcca cagagaaaaa agattgaaaa tccgcaccct ctactacaaa
     1081 caactgttga accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc cttttggaaa
     1141 tctcagatag tgatccgctt ctacgatatt acgtggattc tacgacacat gaaattatac
     1201 tttctttctt agggaaagta caaatggaag tgattagtgc actgttgcaa gaaaagtatc
     1261 atgtggagat agaaataaaa gagcctacag tcatttatat ggaaagaccg ttaaaaaaag
     1321 cagagtatac cattcacatc gaagttccac cgaatccttt ctgggcttcc attggtctat
     1381 ctgtagcaca gcttccatta gggagcggag tacagtatga gagctcggtt tctcttggat
     1441 acttaaatca atcgtttcaa aatgcagtta tggaggggat acgctatggc tgtgaacaag
     1501 gattgtatgg ttggaatgtg acggactgta aaatctgttt taagtatggc ttatactata
     1561 gccctgttag taccccagca gattttcgga tgcttgctcc tattgtattg gaacaagtct
     1621 taaaaaaagc tggaacagaa ttgttagagc catatcttag ttttaaaatt tatgcgccac
     1681 aggaatatct ttcacgagca tacaacgatg ctcctaaata ttgtgcgaac atcgtagaca
     1741 ctcaattgaa aaataatgag gtcattctta gtggagaaat ccctgctcgg tgtattcaag
     1801 aatatcgtag tgatttaact ttctttacaa atggacgtag tgtttgttta acagagttaa
     1861 aagggtacca tgttactacc ggtgaacctg tttgccagcc ccgtcgtcca aatagtcgga
     1921 tagataaagt acgatatatg ttcaataaaa taacttagtg tattttatgt tgttatataa
     1981 atatggtttc ttgttaaata agatgaaata ttttttaata aagatttgaa ttaaagtgta
     2041 aaggaggaga tagttattat aaactacaag tggatattgt gtcctgtatg tggaaataaa
     2101 acacgattaa agataaggga agatactgaa ttaaaaaaat tccccctcta ttgtccgaaa
     2161 tgcagacaag aaaatttaat tgaaataaag cagttcaaag taactgtgat tacagagcca
     2221 gacgcaaaga cgcagagccg ataaaatgag attaatacaa tctcatttta tcggctcttt
     2281 ccgttatgta tggattcttt taattagtct tcgatgtttc ttgcttcgtt gataccgctg
     2341 gctaaagatt ccattaagga tagttctttg tctgtaaagc tatccatgta tttctctatc
     2401 tgtaatcgtc gggtgctttt taccaagtta ttagcaggta agaaaaattc atcaacggaa
     2461 acatgaagta acgatacaag gtcataaaga acttgtatgc tggggtgttg ccctttattt
     2521 tcaatattag ttaagtaccg tgggtcaatt tcaatcaatg ctcccacttg ttcacgagtt
     2581 aaacctcgtt tcaatcgagc ttctttaatg gctaaaccaa aggctctaaa atcatattta
     2641 tcttcttttt tacgcatagt agaccacctc tatacatttt attgttccta ctgaattaaa
     2701 aacaggtata gaaaaacgtg ttatatggtt tataggttta tatttaataa aaagcactac
     2761 taaacgccaa taaaaaaaac cgttatatgg tagtgctatt tacgctgtta aaatattgta
     2821 tattacttcc aaatggcggt ttgttggagg tcaacgtcgc catgaagtac atcatataca
     2881 ataaatttcc ttacattggg ttcttgtcaa aaaaagtcgt ctatctgcaa tagataagta
     2941 cgtccaccaa tgtggtttta taaatcatat agatagaata acagaagcat gtaaacagag
     3001 aaataaatct gtttatatgc ttttttggct attcagaact tttttacaaa gtttatttat
     3061 cagtaatgca acaaatcccc ctttcacatt gggactaaga gtgaaaggag ataaacgagc
     3121 aaggctcact tcctttccta gacagaaagg gggtgagaaa catgaaacca tcttcttttc
     3181 agaccacaat agaaaatcag tttgactata tctgtaaacg tgctatggaa gacgagcgaa
     3241 agaattatat gctttatctt tcaaggattg caaagcgtga ggtgtccttt tcggatgttg
     3301 gcgattatct tgttagccag tttgcgacaa cagataacta ttcaactgac tttcagattt
     3361 ttacactcaa tgggttatca gtaggcgttg aaaatgattt gttgagtgaa gcattacgtg
     3421 agttgccaga caagaaacgt gaaattctac tgctgtttta cttatgggaa tttcgctatt
     3481 tttagaattg cctgcaatag tttcctactg ggtagttaca gtgatgtgct ttgtggctat
     3541 gggtgtatct actatgtttg ttgttcaaat ttacactctt gttcagacac aaacaccacc
     3601 gcaattagta ggaaagataa tggctacgct ggtagcagtt gctatgtgcg gtcaacctat
     3661 tggacaaatg gtttacggta ttttattcga tgtatttata caagaaacat gggttgttat
     3721 gtttgtagct tctattgctt caatcatgat ttcaatatat tcaaaaagaa tttttcaaaa
     3781 gttagagata taatacaagg ggttaaatag tatgttaggc taaaaacaat atataaaaga
     3841 aaatttgaaa tctgctaaaa ggatagtgtt ataaaagaaa gagtaatatt gcagtattta
     3901 aggctcgcac aaatatatgt tttgtgtggg cttttttctt accatttttc tgtatttcta
     3961 aagggtataa acggtattcc gtttatatat ttcagcatta gcagaaaggg ggtgagatta
     4021 tgaggacatc ttctttcgag catatcgtca gattacagtt caattcttta atgctgattg
     4081 tcattaagaa caaagtgaaa agtcgaaaca gacagcttgc aagacattct aaacgtgaag
     4141 tattgttttg tgaaatagcc gaaacgaaac aagttgaacg tggaacgatt gatacctgtt
     4201 tttatgattg tgtatcattt aaagtcttgc actttacaat ttatgtatct gatgaaacac
     4261 ttggtacagt tttacataga ctgtcagaaa aacagcgtag tgcgatttta ttacgttatt
     4321 ttcaaggaat gaacgacaga aaaatttcag aactgtacca tgtatcacgt tctgctattt
     4381 gcagtagaag aactagagga ctaaggaagt taaaaatgct tttgagcgaa gggaagtaaa
     4441 gaaatgagta aagattatgg ctatccctct tttgaattga tttgcaaagc gtcaagcgga
     4501 gatgaaatgg cagttaaaga gattttgaag ttttatgacg gttatatttc taaattatgc
     4561 ttgcgtcctt tttaccactc tgaaagtggt aaaatcatta tgcaggttga tgaagaacta
     4621 aaaggagaaa ttcatacaga catgatgaaa gcaatattga aatttgaaat cggagtgaag
     4681 taatcaactg tataaaaaaa tcgggagata cacagtttcg tgctatctcc cttagtagtt
     4741 ttacggtcat atcaaggctc attcaatcgt ggtaaattcg tggtaagatg agtgtttttc
     4801 tatacttcaa aatgcttgaa tgtatagtgt ttatgcggat aattgaaaat tagatataaa
     4861 atttctatta atcattcata tgatctctag aacgttcaat gatttttcca gtttctgcat
     4921 caatttgata atcaacttca tttttatttt tcaggtccac aaagtcaata tcataaactg
     4981 attttccatt ttcaatttct tctttgatct taagattact gacttctgat tcctctatat
     5041 tagcatcttt aaatgcaatc tttttggctt cttcttgact tattttatga gatttttgac
     5101 ttgacgatga acttgactga gaagtcgatt ttgaaaagcc attattatcc atgtcccgct
     5161 caaggatttg tccagtctta gcatctacat gatagctgta ttctgcgctt ttggtattga
     5221 attcaatttc gtagctccct ctaaagtttt caaattcttt gtcaattttt tgatgtatga
     5281 catccttctt agccacttta gcatctttat aagcaatagc taacgcttga ttagttgtct
     5341 tttggaattg tgattggtaa aataatacac cacctccaag gataacaaca aggactaaaa
     5401 gaactcctaa aaaaacttta tttttcttaa tcatacagca ccttctttct attattcaat
     5461 tgtattttaa cataagctag acactttttc aattttgacc actcgtttat aaaaatatgt
     5521 ggtcaataaa aaccatttga cattaatacc gcaaattaaa agataccata gtatcatggt
     5581 atcttttaat agtatatgta tcagttgatg cgcattttct gcacagcctt cttcaagtat
     5641 ttgacttgtt tatcaaccga ttgcttagca ctacgccttc caaatgcgta atatcctttt
     5701 ttattgaaat aactacaatc gtaatcctct tctttaaaga aaaaccattt ccttctagat
     5761 agatttggcg atctataacc tctagtcata tcgtttcgaa attttgggtc taactttaca
     5821 ggatcaacat ctaattcttt atgacgtgta ttatttttaa aagcataatt aaagctagca
     5881 ccgttgataa tgccattacg tgtgattact tctgcgtcaa tctcgtttaa aaagtttcct
     5941 ttgtcatcaa taataaattc cgtatggaat gaaattaaaa gcttactatt aataaccggc
     6001 tcatcatctg ggaaaccaaa gatttcacca tttctccgac gtttattatg caagcgagca
     6061 gattcatagt aggcgatact atctggttct cttaaacatg ataggtagtt tactaaagca
     6121 tctctatcag tcataccaac atctttaaag tatcgtcgaa catactgtgc ttgctgacta
     6181 gaaatcaaat agcggaattg atggagtttc ctatttaata cagtatcttc ggctagacta
     6241 ttcccaggaa aagaatcata aataacttgt gataaggcta tccagaaatc atcatgcgga
     6301 gctaactcat ctgaaaactg tgcgaccaag tattgtgttt ctgataattc tcctgacaat
     6361 tcagttggca ttttagcctg ctctaacacg aattctaaag ctttgagggg tgcttgtaaa
     6421 cttaaagaag tccaaaaata cttaaaaata ggagagccaa cttttatatc atcaatagcc
     6481 ttgagtccct ttaaaaagag taatagatca tctttaggta tgctccattt atctgctaaa
     6541 gacagtatca tttttatatt ttgataataa ttttctatcc cttcaactgt aaaaggaata
     6601 ttaagagcgg tctttgccat atctctcccc tccaatttta cttattatag caaaaaggaa
     6661 agcttaaaac caagaaatat cttcactaaa aaatggctct cactagccat tatgttttta
     6721 aacaagatac tgcggttttt cgattttata ccctactcct ctaagtgttt ttatatattt
     6781 aggat
//
LOCUS       DATROD010000219         6785 bp    DNA     linear   BCT 26-DEC-2023
DEFINITION  TPA_asm: Streptococcus agalactiae isolate Infant
            SAMN09699814-rid18550813.guided.091, whole genome shotgun sequence.
ACCESSION   DATROD010000219 DATROD010000000
VERSION     DATROD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN09699814
            Sequence Read Archive: SRR7789199
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus agalactiae
  ORGANISM  Streptococcus agalactiae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-JUN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001790070.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001790070.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 06/25/2023 21:18:02
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,087
            CDSs (total)                      :: 2,048
            Genes (coding)                    :: 1,981
            CDSs (with protein)               :: 1,981
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 41 of 67
            Pseudo Genes (incomplete)         :: 16 of 67
            Pseudo Genes (internal stop)      :: 21 of 67
            Pseudo Genes (multiple problems)  :: 10 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Streptococcus agalactiae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN09699814-rid18550813.guided.091"
                     /serotype="III"
                     /isolate="Infant"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1311"
                     /country="South Africa: Johannesburg"
                     /lat_lon="26.2612 S 27.9426 E"
                     /collection_date="2015-02-05"
                     /collected_by="RMPRU"
     gene            39..1958
                     /gene="tet(M)"
                     /locus_tag="VBC74_002079"
     CDS             39..1958
                     /gene="tet(M)"
                     /locus_tag="VBC74_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEO6942962.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYTSGKLLEALELEQEESIRFHNCSLFPVYH
                     GSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIEIKEPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVA
                     QLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2077..2244
                     /locus_tag="VBC74_002080"
     CDS             2077..2244
                     /locus_tag="VBC74_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEO6942963.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2304..2657)
                     /locus_tag="VBC74_002081"
     CDS             complement(2304..2657)
                     /locus_tag="VBC74_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6942964.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            3162..3485
                     /locus_tag="VBC74_002082"
     CDS             3162..3485
                     /locus_tag="VBC74_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEO6942965.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYLW
                     EFRYF"
     gene            3464..3793
                     /locus_tag="VBC74_002083"
     CDS             3464..3793
                     /locus_tag="VBC74_002083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEO6942966.1"
                     /translation="MGISLFLELPAIVSYWVVTVMCFVAMGVSTMFVVQIYTLVQTQT
                     PPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVASIASIMISIYSKR
                     IFQKLEI"
     gene            4020..4439
                     /locus_tag="VBC74_002084"
     CDS             4020..4439
                     /locus_tag="VBC74_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEO6942967.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDRKISELYHVSRSAICSRRTRGLRKLKMLLSEGK"
     gene            4444..4683
                     /locus_tag="VBC74_002085"
     CDS             4444..4683
                     /locus_tag="VBC74_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEO6942968.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(4868..5434)
                     /locus_tag="VBC74_002086"
     CDS             complement(4868..5434)
                     /locus_tag="VBC74_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEO6942969.1"
                     /translation="MIKKNKVFLGVLLVLVVILGGGVLFYQSQFQKTTNQALAIAYKD
                     AKVAKKDVIHQKIDKEFENFRGSYEIEFNTKSAEYSYHVDAKTGQILERDMDNNGFSK
                     STSQSSSSSSQKSHKISQEEAKKIAFKDANIEESEVSNLKIKEEIENGKSVYDIDFVD
                     LKNKNEVDYQIDAETGKIIERSRDHMND"
     gene            complement(5601..6620)
                     /locus_tag="VBC74_002087"
     CDS             complement(5601..6620)
                     /locus_tag="VBC74_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEO6942970.1"
                     /translation="MAKTALNIPFTVEGIENYYQNIKMILSLADKWSIPKDDLLLFLK
                     GLKAIDDIKVGSPIFKYFWTSLSLQAPLKALEFVLEQAKMPTELSGELSETQYLVAQF
                     SDELAPHDDFWIALSQVIYDSFPGNSLAEDTVLNRKLHQFRYLISSQQAQYVRRYFKD
                     VGMTDRDALVNYLSCLREPDSIAYYESARLHNKRRRNGEIFGFPDDEPVINSKLLISF
                     HTEFIIDDKGNFLNEIDAEVITRNGIINGASFNYAFKNNTRHKELDVDPVKLDPKFRN
                     DMTRGYRSPNLSRRKWFFFKEEDYDCSYFNKKGYYAFGRRSAKQSVDKQVKYLKKAVQ
                     KMRIN"
BASE COUNT         2228 a         1089 c         1234 g         2234 t
ORIGIN      
        1 tttatgccct tttgggcttt tgaatggagg aaaatcacat gaaaattatt aatattggag
       61 ttttagctca tgttgatgcg ggaaaaacta ccttaacaga aagcttatta tataacagtg
      121 gagcgattac agaattagga agcgtggaca aaggtacaac gaggacggat aatacgcttt
      181 tagaacgtca gagaggaatt acaattcaga caggaataac ctcttttcag tgggaaaata
      241 cgaaggtgaa catcatagac acgccaggac atatggattt cttagcagaa gtatatcgtt
      301 cattatcagt tttagatggg gcaattctac tgatttctgc aaaagatggc gtacaagcac
      361 aaactcgtat attatttcat gcacttagga aaatggggat tcccacaatc ttttttatca
      421 ataagattga ccaaaatgga attgatttat caacggttta tcaggatatt aaagagaaac
      481 tttctgccga aattgtaatc aaacagaagg tagaactgta tcctaatatg tgtgtgacga
      541 actttaccga atctgaacaa tgggatacgg taatagaggg aaacgatgac cttttagaga
      601 aatatacgtc tgggaaatta ttggaagcat tagaactcga acaagaggaa agcataagat
      661 ttcataattg ttccctgttc cctgtttatc acggaagtgc aaaaaacaat atagggattg
      721 ataaccttat agaagtgatt acgaataaat tttattcatc aacacatcga ggtcagtctg
      781 aactttgcgg aaaagttttc aaaattgagt attcggaaaa aagacagcgt cttgcatata
      841 tacgtcttta tagtggcgta ctgcatttgc gagattcggt tagaatatcg gaaaaggaaa
      901 aaataaaaat tacagaaatg tatacttcaa taaatggtga attatgtaaa atcgataagg
      961 cttattccgg ggaaattgtt attttgcaaa atgagttttt gaagttaaat agtgttcttg
     1021 gagatacaaa actattgcca cagagaaaaa agattgaaaa tccgcaccct ctactacaaa
     1081 caactgttga accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc cttttggaaa
     1141 tctcagatag tgatccgctt ctacgatatt acgtggattc tacgacacat gaaattatac
     1201 tttctttctt agggaaagta caaatggaag tgattagtgc actgttgcaa gaaaagtatc
     1261 atgtggagat agaaataaaa gagcctacag tcatttatat ggaaagaccg ttaaaaaaag
     1321 cagagtatac cattcacatc gaagttccac cgaatccttt ctgggcttcc attggtctat
     1381 ctgtagcaca gcttccatta gggagcggag tacagtatga gagctcggtt tctcttggat
     1441 acttaaatca atcgtttcaa aatgcagtta tggaggggat acgctatggc tgtgaacaag
     1501 gattgtatgg ttggaatgtg acggactgta aaatctgttt taagtatggc ttatactata
     1561 gccctgttag taccccagca gattttcgga tgcttgctcc tattgtattg gaacaagtct
     1621 taaaaaaagc tggaacagaa ttgttagagc catatcttag ttttaaaatt tatgcgccac
     1681 aggaatatct ttcacgagca tacaacgatg ctcctaaata ttgtgcgaac atcgtagaca
     1741 ctcaattgaa aaataatgag gtcattctta gtggagaaat ccctgctcgg tgtattcaag
     1801 aatatcgtag tgatttaact ttctttacaa atggacgtag tgtttgttta acagagttaa
     1861 aagggtacca tgttactacc ggtgaacctg tttgccagcc ccgtcgtcca aatagtcgga
     1921 tagataaagt acgatatatg ttcaataaaa taacttagtg tattttatgt tgttatataa
     1981 atatggtttc ttgttaaata agatgaaata ttttttaata aagatttgaa ttaaagtgta
     2041 aaggaggaga tagttattat aaactacaag tggatattgt gtcctgtatg tggaaataaa
     2101 acacgattaa agataaggga agatactgaa ttaaaaaaat tccccctcta ttgtccgaaa
     2161 tgcagacaag aaaatttaat tgaaataaag cagttcaaag taactgtgat tacagagcca
     2221 gacgcaaaga cgcagagccg ataaaatgag attaatacaa tctcatttta tcggctcttt
     2281 ccgttatgta tggattcttt taattagtct tcgatgtttc ttgcttcgtt gataccgctg
     2341 gctaaagatt ccattaagga tagttctttg tctgtaaagc tatccatgta tttctctatc
     2401 tgtaatcgtc gggtgctttt taccaagtta ttagcaggta agaaaaattc atcaacggaa
     2461 acatgaagta acgatacaag gtcataaaga acttgtatgc tggggtgttg ccctttattt
     2521 tcaatattag ttaagtaccg tgggtcaatt tcaatcaatg ctcccacttg ttcacgagtt
     2581 aaacctcgtt tcaatcgagc ttctttaatg gctaaaccaa aggctctaaa atcatattta
     2641 tcttcttttt tacgcatagt agaccacctc tatacatttt attgttccta ctgaattaaa
     2701 aacaggtata gaaaaacgtg ttatatggtt tataggttta tatttaataa aaagcactac
     2761 taaacgccaa taaaaaaaac cgttatatgg tagtgctatt tacgctgtta aaatattgta
     2821 tattacttcc aaatggcggt ttgttggagg tcaacgtcgc catgaagtac atcatataca
     2881 ataaatttcc ttacattggg ttcttgtcaa aaaaagtcgt ctatctgcaa tagataagta
     2941 cgtccaccaa tgtggtttta taaatcatat agatagaata acagaagcat gtaaacagag
     3001 aaataaatct gtttatatgc ttttttggct attcagaact tttttacaaa gtttatttat
     3061 cagtaatgca acaaatcccc ctttcacatt gggactaaga gtgaaaggag ataaacgagc
     3121 aaggctcact tcctttccta gacagaaagg gggtgagaaa catgaaacca tcttcttttc
     3181 agaccacaat agaaaatcag tttgactata tctgtaaacg tgctatggaa gacgagcgaa
     3241 agaattatat gctttatctt tcaaggattg caaagcgtga ggtgtccttt tcggatgttg
     3301 gcgattatct tgttagccag tttgcgacaa cagataacta ttcaactgac tttcagattt
     3361 ttacactcaa tgggttatca gtaggcgttg aaaatgattt gttgagtgaa gcattacgtg
     3421 agttgccaga caagaaacgt gaaattctac tgctgtttta cttatgggaa tttcgctatt
     3481 tttagaattg cctgcaatag tttcctactg ggtagttaca gtgatgtgct ttgtggctat
     3541 gggtgtatct actatgtttg ttgttcaaat ttacactctt gttcagacac aaacaccacc
     3601 gcaattagta ggaaagataa tggctacgct ggtagcagtt gctatgtgcg gtcaacctat
     3661 tggacaaatg gtttacggta ttttattcga tgtatttata caagaaacat gggttgttat
     3721 gtttgtagct tctattgctt caatcatgat ttcaatatat tcaaaaagaa tttttcaaaa
     3781 gttagagata taatacaagg ggttaaatag tatgttaggc taaaaacaat atataaaaga
     3841 aaatttgaaa tctgctaaaa ggatagtgtt ataaaagaaa gagtaatatt gcagtattta
     3901 aggctcgcac aaatatatgt tttgtgtggg cttttttctt accatttttc tgtatttcta
     3961 aagggtataa acggtattcc gtttatatat ttcagcatta gcagaaaggg ggtgagatta
     4021 tgaggacatc ttctttcgag catatcgtca gattacagtt caattcttta atgctgattg
     4081 tcattaagaa caaagtgaaa agtcgaaaca gacagcttgc aagacattct aaacgtgaag
     4141 tattgttttg tgaaatagcc gaaacgaaac aagttgaacg tggaacgatt gatacctgtt
     4201 tttatgattg tgtatcattt aaagtcttgc actttacaat ttatgtatct gatgaaacac
     4261 ttggtacagt tttacataga ctgtcagaaa aacagcgtag tgcgatttta ttacgttatt
     4321 ttcaaggaat gaacgacaga aaaatttcag aactgtacca tgtatcacgt tctgctattt
     4381 gcagtagaag aactagagga ctaaggaagt taaaaatgct tttgagcgaa gggaagtaaa
     4441 gaaatgagta aagattatgg ctatccctct tttgaattga tttgcaaagc gtcaagcgga
     4501 gatgaaatgg cagttaaaga gattttgaag ttttatgacg gttatatttc taaattatgc
     4561 ttgcgtcctt tttaccactc tgaaagtggt aaaatcatta tgcaggttga tgaagaacta
     4621 aaaggagaaa ttcatacaga catgatgaaa gcaatattga aatttgaaat cggagtgaag
     4681 taatcaactg tataaaaaaa tcgggagata cacagtttcg tgctatctcc cttagtagtt
     4741 ttacggtcat atcaaggctc attcaatcgt ggtaaattcg tggtaagatg agtgtttttc
     4801 tatacttcaa aatgcttgaa tgtatagtgt ttatgcggat aattgaaaat tagatataaa
     4861 atttctatta atcattcata tgatctctag aacgttcaat gatttttcca gtttctgcat
     4921 caatttgata atcaacttca tttttatttt tcaggtccac aaagtcaata tcataaactg
     4981 attttccatt ttcaatttct tctttgatct taagattact gacttctgat tcctctatat
     5041 tagcatcttt aaatgcaatc tttttggctt cttcttgact tattttatga gatttttgac
     5101 ttgacgatga acttgactga gaagtcgatt ttgaaaagcc attattatcc atgtcccgct
     5161 caaggatttg tccagtctta gcatctacat gatagctgta ttctgcgctt ttggtattga
     5221 attcaatttc gtagctccct ctaaagtttt caaattcttt gtcaattttt tgatgtatga
     5281 catccttctt agccacttta gcatctttat aagcaatagc taacgcttga ttagttgtct
     5341 tttggaattg tgattggtaa aataatacac cacctccaag gataacaaca aggactaaaa
     5401 gaactcctaa aaaaacttta tttttcttaa tcatacagca ccttctttct attattcaat
     5461 tgtattttaa cataagctag acactttttc aattttgacc actcgtttat aaaaatatgt
     5521 ggtcaataaa aaccatttga cattaatacc gcaaattaaa agataccata gtatcatggt
     5581 atcttttaat agtatatgta tcagttgatg cgcattttct gcacagcctt cttcaagtat
     5641 ttgacttgtt tatcaaccga ttgcttagca ctacgccttc caaatgcgta atatcctttt
     5701 ttattgaaat aactacaatc gtaatcctct tctttaaaga aaaaccattt ccttctagat
     5761 agatttggcg atctataacc tctagtcata tcgtttcgaa attttgggtc taactttaca
     5821 ggatcaacat ctaattcttt atgacgtgta ttatttttaa aagcataatt aaagctagca
     5881 ccgttgataa tgccattacg tgtgattact tctgcgtcaa tctcgtttaa aaagtttcct
     5941 ttgtcatcaa taataaattc cgtatggaat gaaattaaaa gcttactatt aataaccggc
     6001 tcatcatctg ggaaaccaaa gatttcacca tttctccgac gtttattatg caagcgagca
     6061 gattcatagt aggcgatact atctggttct cttaaacatg ataggtagtt tactaaagca
     6121 tctctatcag tcataccaac atctttaaag tatcgtcgaa catactgtgc ttgctgacta
     6181 gaaatcaaat agcggaattg atggagtttc ctatttaata cagtatcttc ggctagacta
     6241 ttcccaggaa aagaatcata aataacttgt gataaggcta tccagaaatc atcatgcgga
     6301 gctaactcat ctgaaaactg tgcgaccaag tattgtgttt ctgataattc tcctgacaat
     6361 tcagttggca ttttagcctg ctctaacacg aattctaaag ctttgagggg tgcttgtaaa
     6421 cttaaagaag tccaaaaata cttaaaaata ggagagccaa cttttatatc atcaatagcc
     6481 ttgagtccct ttaaaaagag taatagatca tctttaggta tgctccattt atctgctaaa
     6541 gacagtatca tttttatatt ttgataataa ttttctatcc cttcaactgt aaaaggaata
     6601 ttaagagcgg tctttgccat atctctcccc tccaatttta cttattatag caaaaaggaa
     6661 agcttaaaac caagaaatat cttcactaaa aaatggctct cactagccat tatgttttta
     6721 aacaagatac tgcggttttt cgattttata ccctactcct ctaagtgttt ttatatattt
     6781 aggat
//
